LOCUS       DAKBVG010000001        80611 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000001 DAKBVG010000000
VERSION     DAKBVG010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 80611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80611
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.001"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            <1..411
                     /locus_tag="OUD09_000001"
     CDS             <1..411
                     /locus_tag="OUD09_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9691317.1"
                     /translation="GLYYSPVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAP
                     QEYLSRAYNDAPKYCANIVDTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLT
                     ELKGYHVTTGEPVCQPRRPNSRIDKVRYMFNKIT"
     gene            509..697
                     /locus_tag="OUD09_000002"
     CDS             509..697
                     /locus_tag="OUD09_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HCT9691318.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(755..1108)
                     /locus_tag="OUD09_000003"
     CDS             complement(755..1108)
                     /locus_tag="OUD09_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCT9691319.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            1639..2061
                     /locus_tag="OUD09_000004"
     CDS             1639..2061
                     /locus_tag="OUD09_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCT9691320.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            2058..2288
                     /locus_tag="OUD09_000005"
     CDS             2058..2288
                     /locus_tag="OUD09_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCT9691321.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            2786..2986
                     /locus_tag="OUD09_000006"
     CDS             2786..2986
                     /locus_tag="OUD09_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HCT9691322.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            3013..4206
                     /locus_tag="OUD09_000007"
     CDS             3013..4206
                     /locus_tag="OUD09_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCT9691323.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(4274..5839)
                     /gene="guaA"
                     /locus_tag="OUD09_000008"
     CDS             complement(4274..5839)
                     /gene="guaA"
                     /locus_tag="OUD09_000008"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCT9691324.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(5898..6821)
                     /locus_tag="OUD09_000009"
     CDS             complement(5898..6821)
                     /locus_tag="OUD09_000009"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCT9691325.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            7017..7622
                     /locus_tag="OUD09_000010"
     CDS             7017..7622
                     /locus_tag="OUD09_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCT9691326.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            7930..8946
                     /locus_tag="OUD09_000011"
     CDS             7930..8946
                     /locus_tag="OUD09_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCT9691327.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            9087..10388
                     /locus_tag="OUD09_000012"
     CDS             9087..10388
                     /locus_tag="OUD09_000012"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HCT9691328.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            10434..11096
                     /gene="deoC"
                     /locus_tag="OUD09_000013"
     CDS             10434..11096
                     /gene="deoC"
                     /locus_tag="OUD09_000013"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCT9691329.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            11117..11515
                     /locus_tag="OUD09_000014"
     CDS             11117..11515
                     /locus_tag="OUD09_000014"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCT9691330.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            11581..12663
                     /locus_tag="OUD09_000015"
     CDS             11581..12663
                     /locus_tag="OUD09_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HCT9691331.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            12875..14437
                     /locus_tag="OUD09_000016"
     CDS             12875..14437
                     /locus_tag="OUD09_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT9691332.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            14430..15563
                     /locus_tag="OUD09_000017"
     CDS             14430..15563
                     /locus_tag="OUD09_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT9691333.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            15563..16519
                     /locus_tag="OUD09_000018"
     CDS             15563..16519
                     /locus_tag="OUD09_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT9691334.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            16618..17784
                     /gene="deoB"
                     /locus_tag="OUD09_000019"
     CDS             16618..17784
                     /gene="deoB"
                     /locus_tag="OUD09_000019"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCT9691335.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            17808..18626
                     /locus_tag="OUD09_000020"
     CDS             17808..18626
                     /locus_tag="OUD09_000020"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCT9691336.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            18643..19350
                     /gene="deoD"
                     /locus_tag="OUD09_000021"
     CDS             18643..19350
                     /gene="deoD"
                     /locus_tag="OUD09_000021"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCT9691337.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(19648..20334)
                     /gene="gpmA"
                     /locus_tag="OUD09_000022"
     CDS             complement(19648..20334)
                     /gene="gpmA"
                     /locus_tag="OUD09_000022"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCT9691338.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(20506..21087)
                     /locus_tag="OUD09_000023"
     CDS             complement(20506..21087)
                     /locus_tag="OUD09_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCT9691339.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(21220..21903)
                     /gene="rpiA"
                     /locus_tag="OUD09_000024"
     CDS             complement(21220..21903)
                     /gene="rpiA"
                     /locus_tag="OUD09_000024"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HCT9691340.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            22222..22635
                     /gene="rpsL"
                     /locus_tag="OUD09_000025"
     CDS             22222..22635
                     /gene="rpsL"
                     /locus_tag="OUD09_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCT9691341.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            22719..23189
                     /gene="rpsG"
                     /locus_tag="OUD09_000026"
     CDS             22719..23189
                     /gene="rpsG"
                     /locus_tag="OUD09_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCT9691342.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            23283..25370
                     /gene="fusA"
                     /locus_tag="OUD09_000027"
     CDS             23283..25370
                     /gene="fusA"
                     /locus_tag="OUD09_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCT9691343.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            25542..26729
                     /gene="tuf"
                     /locus_tag="OUD09_000028"
     CDS             25542..26729
                     /gene="tuf"
                     /locus_tag="OUD09_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCT9691344.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            27136..27444
                     /gene="rpsJ"
                     /locus_tag="OUD09_000029"
     CDS             27136..27444
                     /gene="rpsJ"
                     /locus_tag="OUD09_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCT9691345.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            27475..28104
                     /gene="rplC"
                     /locus_tag="OUD09_000030"
     CDS             27475..28104
                     /gene="rplC"
                     /locus_tag="OUD09_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCT9691346.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            28132..28755
                     /gene="rplD"
                     /locus_tag="OUD09_000031"
     CDS             28132..28755
                     /gene="rplD"
                     /locus_tag="OUD09_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCT9691347.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            28755..29045
                     /gene="rplW"
                     /locus_tag="OUD09_000032"
     CDS             28755..29045
                     /gene="rplW"
                     /locus_tag="OUD09_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCT9691348.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            29084..29917
                     /gene="rplB"
                     /locus_tag="OUD09_000033"
     CDS             29084..29917
                     /gene="rplB"
                     /locus_tag="OUD09_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCT9691349.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            29960..30238
                     /gene="rpsS"
                     /locus_tag="OUD09_000034"
     CDS             29960..30238
                     /gene="rpsS"
                     /locus_tag="OUD09_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCT9691350.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            30261..30608
                     /gene="rplV"
                     /locus_tag="OUD09_000035"
     CDS             30261..30608
                     /gene="rplV"
                     /locus_tag="OUD09_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCT9691351.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            30622..31278
                     /gene="rpsC"
                     /locus_tag="OUD09_000036"
     CDS             30622..31278
                     /gene="rpsC"
                     /locus_tag="OUD09_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCT9691352.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            31281..31715
                     /gene="rplP"
                     /locus_tag="OUD09_000037"
     CDS             31281..31715
                     /gene="rplP"
                     /locus_tag="OUD09_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCT9691353.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            31705..31893
                     /gene="rpmC"
                     /locus_tag="OUD09_000038"
     CDS             31705..31893
                     /gene="rpmC"
                     /locus_tag="OUD09_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCT9691354.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            31918..32184
                     /gene="rpsQ"
                     /locus_tag="OUD09_000039"
     CDS             31918..32184
                     /gene="rpsQ"
                     /locus_tag="OUD09_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCT9691355.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            32242..32610
                     /gene="rplN"
                     /locus_tag="OUD09_000040"
     CDS             32242..32610
                     /gene="rplN"
                     /locus_tag="OUD09_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCT9691356.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            32647..32955
                     /gene="rplX"
                     /locus_tag="OUD09_000041"
     CDS             32647..32955
                     /gene="rplX"
                     /locus_tag="OUD09_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCT9691357.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            32983..33522
                     /gene="rplE"
                     /locus_tag="OUD09_000042"
     CDS             32983..33522
                     /gene="rplE"
                     /locus_tag="OUD09_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCT9691358.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            33541..33726
                     /locus_tag="OUD09_000043"
     CDS             33541..33726
                     /locus_tag="OUD09_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HCT9691359.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            33762..34160
                     /gene="rpsH"
                     /locus_tag="OUD09_000044"
     CDS             33762..34160
                     /gene="rpsH"
                     /locus_tag="OUD09_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCT9691360.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            34192..34728
                     /gene="rplF"
                     /locus_tag="OUD09_000045"
     CDS             34192..34728
                     /gene="rplF"
                     /locus_tag="OUD09_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCT9691361.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            34889..35245
                     /gene="rplR"
                     /locus_tag="OUD09_000046"
     CDS             34889..35245
                     /gene="rplR"
                     /locus_tag="OUD09_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCT9691362.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            35266..35766
                     /gene="rpsE"
                     /locus_tag="OUD09_000047"
     CDS             35266..35766
                     /gene="rpsE"
                     /locus_tag="OUD09_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCT9691363.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            35781..35960
                     /gene="rpmD"
                     /locus_tag="OUD09_000048"
     CDS             35781..35960
                     /gene="rpmD"
                     /locus_tag="OUD09_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCT9691364.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            36004..36444
                     /gene="rplO"
                     /locus_tag="OUD09_000049"
     CDS             36004..36444
                     /gene="rplO"
                     /locus_tag="OUD09_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCT9691365.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            36444..37739
                     /gene="secY"
                     /locus_tag="OUD09_000050"
     CDS             36444..37739
                     /gene="secY"
                     /locus_tag="OUD09_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCT9691366.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            37801..38448
                     /locus_tag="OUD09_000051"
     CDS             37801..38448
                     /locus_tag="OUD09_000051"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCT9691367.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            38639..38857
                     /gene="infA"
                     /locus_tag="OUD09_000052"
     CDS             38639..38857
                     /gene="infA"
                     /locus_tag="OUD09_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCT9691368.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            38890..39006
                     /gene="rpmJ"
                     /locus_tag="OUD09_000053"
     CDS             38890..39006
                     /gene="rpmJ"
                     /locus_tag="OUD09_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCT9691369.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            39024..39389
                     /gene="rpsM"
                     /locus_tag="OUD09_000054"
     CDS             39024..39389
                     /gene="rpsM"
                     /locus_tag="OUD09_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCT9691370.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            39417..39806
                     /gene="rpsK"
                     /locus_tag="OUD09_000055"
     CDS             39417..39806
                     /gene="rpsK"
                     /locus_tag="OUD09_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCT9691371.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            39886..40824
                     /locus_tag="OUD09_000056"
     CDS             39886..40824
                     /locus_tag="OUD09_000056"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCT9691372.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            40852..41232
                     /gene="rplQ"
                     /locus_tag="OUD09_000057"
     CDS             40852..41232
                     /gene="rplQ"
                     /locus_tag="OUD09_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCT9691373.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            41504..42343
                     /locus_tag="OUD09_000058"
     CDS             41504..42343
                     /locus_tag="OUD09_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCT9691374.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            42319..43188
                     /locus_tag="OUD09_000059"
     CDS             42319..43188
                     /locus_tag="OUD09_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCT9691375.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            43178..43975
                     /locus_tag="OUD09_000060"
     CDS             43178..43975
                     /locus_tag="OUD09_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCT9691376.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            44178..44924
                     /gene="truA"
                     /locus_tag="OUD09_000061"
     CDS             44178..44924
                     /gene="truA"
                     /locus_tag="OUD09_000061"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCT9691377.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(45257..47926)
                     /locus_tag="OUD09_000062"
     CDS             complement(45257..47926)
                     /locus_tag="OUD09_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HCT9691378.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(48164..48352)
                     /locus_tag="OUD09_000063"
     CDS             complement(48164..48352)
                     /locus_tag="OUD09_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCT9691379.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            48622..48984
                     /locus_tag="OUD09_000064"
     CDS             48622..48984
                     /locus_tag="OUD09_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCT9691380.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            49036..50718
                     /locus_tag="OUD09_000065"
     CDS             49036..50718
                     /locus_tag="OUD09_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HCT9691381.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            50835..52871
                     /gene="recG"
                     /locus_tag="OUD09_000066"
     CDS             50835..52871
                     /gene="recG"
                     /locus_tag="OUD09_000066"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCT9691382.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            52920..53921
                     /gene="plsX"
                     /locus_tag="OUD09_000067"
     CDS             52920..53921
                     /gene="plsX"
                     /locus_tag="OUD09_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCT9691383.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            54043..54285
                     /gene="acpP"
                     /locus_tag="OUD09_000068"
     CDS             54043..54285
                     /gene="acpP"
                     /locus_tag="OUD09_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCT9691384.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            54553..55557
                     /locus_tag="OUD09_000069"
     CDS             54553..55557
                     /locus_tag="OUD09_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT9691385.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            55558..56502
                     /locus_tag="OUD09_000070"
     CDS             55558..56502
                     /locus_tag="OUD09_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT9691386.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            56502..57464
                     /locus_tag="OUD09_000071"
     CDS             56502..57464
                     /locus_tag="OUD09_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT9691387.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            57491..58405
                     /locus_tag="OUD09_000072"
     CDS             57491..58405
                     /locus_tag="OUD09_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT9691388.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            58431..60212
                     /locus_tag="OUD09_000073"
     CDS             58431..60212
                     /locus_tag="OUD09_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HCT9691389.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            60560..61246
                     /locus_tag="OUD09_000074"
     CDS             60560..61246
                     /locus_tag="OUD09_000074"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCT9691390.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            61266..64847
                     /gene="smc"
                     /locus_tag="OUD09_000075"
     CDS             61266..64847
                     /gene="smc"
                     /locus_tag="OUD09_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HCT9691391.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSMKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            64856..65665
                     /locus_tag="OUD09_000076"
     CDS             64856..65665
                     /locus_tag="OUD09_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCT9691392.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            65676..66674
                     /gene="ftsY"
                     /locus_tag="OUD09_000077"
     CDS             65676..66674
                     /gene="ftsY"
                     /locus_tag="OUD09_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCT9691393.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            66861..69128
                     /gene="gshAB"
                     /locus_tag="OUD09_000078"
     CDS             66861..69128
                     /gene="gshAB"
                     /locus_tag="OUD09_000078"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HCT9691394.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(69217..69669)
                     /locus_tag="OUD09_000079"
     CDS             complement(69217..69669)
                     /locus_tag="OUD09_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HCT9691395.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            69819..70769
                     /locus_tag="OUD09_000080"
     CDS             69819..70769
                     /locus_tag="OUD09_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HCT9691396.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            70762..71721
                     /locus_tag="OUD09_000081"
     CDS             70762..71721
                     /locus_tag="OUD09_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HCT9691397.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            71718..72473
                     /locus_tag="OUD09_000082"
     CDS             71718..72473
                     /locus_tag="OUD09_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCT9691398.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            72496..73449
                     /locus_tag="OUD09_000083"
     CDS             72496..73449
                     /locus_tag="OUD09_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HCT9691399.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(74093..74389)
                     /locus_tag="OUD09_000084"
     CDS             complement(74093..74389)
                     /locus_tag="OUD09_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HCT9691400.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            74744..75100
                     /locus_tag="OUD09_000085"
     CDS             74744..75100
                     /locus_tag="OUD09_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HCT9691401.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            75110..76009
                     /locus_tag="OUD09_000086"
     CDS             75110..76009
                     /locus_tag="OUD09_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HCT9691402.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            76526..77257
                     /locus_tag="OUD09_000087"
     CDS             76526..77257
                     /locus_tag="OUD09_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HCT9691403.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            77402..79429
                     /locus_tag="OUD09_000088"
     CDS             77402..79429
                     /locus_tag="OUD09_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HCT9691404.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            79537..>80611
                     /locus_tag="OUD09_000089"
     CDS             79537..>80611
                     /locus_tag="OUD09_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HCT9691405.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLR"
BASE COUNT        26364 a        14500 c        17252 g        22495 t
ORIGIN      
        1 ggcttatact atagccctgt tagtacccca gcagattttc ggatgcttgc tcctattgta
       61 ttggaacaag tcttaaaaaa agctggaaca gaattgttag agccatatct tagttttaaa
      121 atttatgcgc cacaggaata tctttcacga gcatacaacg atgctcctaa atattgtgcg
      181 aacatcgtag acactcaatt gaaaaataat gaggtcattc ttagtggaga aatccctgct
      241 cggtgtattc aagaatatcg tagtgattta actttcttta caaatggacg tagtgtttgt
      301 ttaacagagt taaaagggta ccatgttact accggtgaac ctgtttgcca gccccgtcgt
      361 ccaaatagtc ggatagataa agtacgatat atgttcaata aaataactta gtgtatttta
      421 tgttgttata taaatatggt ttcttattaa ataagatgaa atattcttta atatagattt
      481 gaattaaagt ggaaaggagg agattgttat tataaactac aagtggatat tgtgtcctat
      541 ttgtggaaat aaaacaagac tacgaatacg agtggatact atacttaaaa atttcccttt
      601 atactgcccc aaatgtaaga acgaaacttt aattaatgtt caaaaaatga atataataac
      661 aatcaaagag ccagacgcca agacgcagag ccgataattt gagaaatgaa actctcatct
      721 tatcggctct ttttgtttat ctgaatttta ctgactagcc ttcaatattt cttgcttcat
      781 tgataccttt tgctagtgct tccattaagg atagttcttt gtctgtaaag ctatccatgt
      841 atttttctac ctgtaacctt cgagtgcttt taatcaaatt atctgtaggt aagaaaaatt
      901 catcaataga tacatggagt aatgatacaa gatcatacag cacttgtgta cttgggtgtt
      961 gtcctttgtt ttctatattt gttaaataac gtgggtcaat ttcaatcatt gttcccactt
     1021 gttcacgagt gagaccccgt ttcattcgag cctctttaat cgctaaacca acggctctaa
     1081 aatcgtattt atcttctttt ttacgcatac taaatcacct ctatacattt tactgttcct
     1141 atataaccat aaacaggtat agaaaaacat gttatatagt ttatgagttc ctatagaaca
     1201 ctgtggaatt tattaatgcg atgaatccaa tttcaaatag aaaggtcgct gattttattt
     1261 acctatacaa ctttataaaa tttagtcata ctcctagaaa gtagataaaa ttggcgactt
     1321 aatggaggga aaagtcgcca tgagaaagat aatctgtcat ctagttctct taattaagtt
     1381 cttatcaaaa aaacttatcc acctgtactg gatagatatg accatcaatg tggttttgca
     1441 gattacatag atagaaaaat caaagcatat gaacagagaa aacaatctgt ttatgtgctt
     1501 ttttggtcac ttaataaata tcttttagga tttctcttca tttatgcaac aaatcccccg
     1561 ttcgtgtcgg ggtttagtat gaagggagat aaagaagcac atgccactcc ctttcacgac
     1621 agaaaggggg tgaaaagcat gaaaccatct tcttttcaag aaaccataga aaatcagttt
     1681 gactatatct gcaaacaagt tattgaagat gaacgaaaag attacttcaa acatctagac
     1741 agactatcag caaaagaagt ctcgttttct gatatagata actaccttgt caactctttt
     1801 tctactgttg atacgtacgt cactgatttc caactattta aattatttgg attaagaatc
     1861 aatgttgaaa gtgatttatt aagtgaagcc ctgcgtcatc tttcagagaa aaaacggaac
     1921 attattttac ttcattactt tatggatatg agtgatgtgg agattgcgga gttactaaat
     1981 ttgaatcgtt cgactgttta tcgtcatcga ataagtggac tagccatgat taaagaattc
     2041 atgaaggagt gtaaagaatg aaaacagctt atccaagggt tcccttcccc ctcattgtaa
     2101 aggcgacaga tggcgacgtg gaagcaatta atcagattgt taaacattat cgagggtata
     2161 cctcaaaacg ttcattacgt cgcatgacag acgaatatgg aaattctcat atggttattg
     2221 atgaaaccct acgtggcaga atggaaacga aactcatctc taagatttta tcgttcgaaa
     2281 ttaaatagtc ccattttttc gtggaagcgt gtcgaatcgt atccacgctt cccgatttgg
     2341 gaggtttgtt attccatcaa agcatattgt gtgttcagta ggttttgata ggctaacaag
     2401 ccaaacgttc tttgacaacc aaataaaggc aatcgaatac gtttcggtaa gaaaagagct
     2461 gatgaactaa cacgccatga cctacaaaat tagtgagcga ttcatgttag taattcgaaa
     2521 gtaatcttta gcaggattgc tgccatgact ttcttatcgt gataatgata ctcccgtata
     2581 gccatagtcc gagcgttaga agcgtcacag gcaatgggta cggctacatg agaaccatgc
     2641 agaagtgaaa ttcctgtgag ctttgctaga gctgttcgat tgctaagata ttcttctctt
     2701 gtcagtgtat tattataaaa ctatttgata taattatagg tgaatattgg agttttccta
     2761 gcctataatt gggaaaggag taataatgat aacggtcaca aaaaaggatt tagttgagct
     2821 aggatatgga acttcttttt cagcagacat cataaaaaaa gcaaaagaat tgatgattac
     2881 aaaagggcat atctactatc aatctcgaaa gctagataga gtccctcgtg aagctgttga
     2941 agaaattcta ggaattcggt tttcaaatga aaacaggtct gagtagtttt tgcacagtaa
     3001 ggagtaaaaa ctatggttaa agacccaata aaaaaagcaa agaatggaac ctattatttt
     3061 cgtgcaaatc taggttatga ttctaaaggg aaaaaaattc aaaaatatcg tagtggattc
     3121 actactaaaa aagaagcaag agaagaatat tcgaaactgc tattgatgaa accagaagaa
     3181 ttaagtgaga ataaggataa aatgacgttt aaacattata tctttgaaat tttccttcca
     3241 tggtataaga cacaggtaaa gctcagaaca tatgaaaatc gcttaccaac tattaagaag
     3301 catttctctt attttgataa aatggctgtt tccgatattg agcctattga tgtgcaaaat
     3361 tggcaactaa aattatcaaa gaagtgtaag tcttcttatg taagagctgt tcaaggacta
     3421 ttttcagttg ctatggatcg agcaattgtc cttgggataa ccaccacaaa tccatctaag
     3481 attattggca atgtgaaaaa gcaaaagtct aaaattgaat tttggacaaa agaagaattt
     3541 gaaaaagtaa tctctctaat ttacaaagaa gattactatc aacacttttt atttatctct
     3601 ctgtggtttt tatttatgac tggaatgcga attggtgaag ctactgccat tcaatgggaa
     3661 gatattgatt ttgactcagg agtactaacc atcaacaaaa cactctatta caagaatcaa
     3721 tcgaattata gatttgttga accaaaaaca aaggcgagcg ttcgtcatat tgtattagat
     3781 aaatgcacat taacttattt atccgaatgg aaggaagtac aacaaaatct aattcagaca
     3841 gactttgtaa tgagctataa cggtattcct acccaaaaac atacactatc atatgctatt
     3901 gaacgctatg ctaagctggc tggtatccat cgaatcagaa tccatgggtt acggcattct
     3961 catgcttcct tgttaatcag tatgggagaa aatccactta tcattaaaga tcgtctagga
     4021 cacgaagata ttgagacgac tttaggaacg tatggacatc tttatcctaa cagtaatttc
     4081 gaggtagcta acaaattaaa tggagccatt tcctttaaag aagcaaaaca aaattatgat
     4141 acttcaccaa agaatcaatt tactgtagac tacttgcgaa acaaagaaat gaaaaaagtg
     4201 caataaaagt gcagtaaaaa gataagaaga gccagaaacc cttggtataa gaggatttct
     4261 agctctccta caactattcc cactccactg ttgctggtgg tttagaagtg atgtcataca
     4321 cgatacggtt gacgtgggct acttcgttta cgatacgtac agagattttt tgcaagactt
     4381 cccatgggat acgggcaaag tcagcggtca ttccgtcgat tgaagttaca gcacggatac
     4441 cgactgtgta gtcgtaagta cgtccgtcac ccataacacc gacagagcgg atccctggaa
     4501 gaacagtgaa atattgccag atatcacggt caagtccagc agctgcgatt tcttcacgca
     4561 agatagcatc agattcgcgt acgatttcta gcttgtcttc agtgatttcg ccaagaacac
     4621 gaataccaag accaggacca gggaatggct gacgccaaac gattgaatca ggcatgccta
     4681 actgagtccc taattcacgg acttcgtctt tgaataatgt gtttaatggt tcgatcaatt
     4741 caaattgcat atcttctggc agtccaccta cattatggtg agatttgatc gtctgtgctg
     4801 tttctgtacc agattcgata acgtcagtat acaacgttcc ttgtgctagg aaagagacgc
     4861 cttcttcacc agcaagtttt gttgcttcgt catcaaaaac atatacgaat tcattcccaa
     4921 tgattttacg tttttgttct gggtcagata cacctttcaa cttgtctaag aagcgatctc
     4981 ttgcatcgac tttgatgatg ttcaagccga attttccgcc tagactttcc attacttgtt
     5041 ccgcttcgcc tttgcgtagc agaccgtggt caacaaagat agatgtcaat tggtcaccga
     5101 tcgctttttg taaaagaacc ccaacgacac tagaatcaac gccacctgaa agaccaagca
     5161 agactttttt gtcaccaact tgttcacgga ttttttctac ttgcatatcg ataaagttat
     5221 ccattgtcca gttaccttca cagtgacaaa catcaaaggc aaagtggcgc agcaattcga
     5281 ttccgtattc ggaatgacgt acttctgcat ggaattggat accataaaaa ttacgtgttg
     5341 tgtcttggat agaagcaatc ggacaatctt tgcttgttcc gactgtttca aagccttcag
     5401 gaactttagt taccaagtca ccatggctca tccatactgt ttgttgtttt ggtgtttctt
     5461 taaacaagac agcttgatcg cttgttactt ctaacaaggc tttcccatat tcgcggtttt
     5521 tagcaggctc tacggaaccg ccaagtttgt acgtgatcaa ttgcatacca tagcaaatcc
     5581 ctaatacagg aatacctaaa ttgaagatct caggatcgat cccaaatgca ttctcatcat
     5641 atacactgtt tggtccgcca gaaagaatga ttccctttgg attcatcgct tttacttctt
     5701 ctgcagtaat gcggtggctc aataattcgg aaaagactcc aagatcacga atacgtcttg
     5761 tgatcaattg gttatactga ctaccgtagt ctaatacgat aatcttttca acgttcgtca
     5821 tatcggcaac gtttgtcaaa tttattaccc ctatcccttt aaattttatc tcaaaatagt
     5881 caaactattt taaaatgcta atattttcgc atgaaaatcg aatcgatgat atgattttca
     5941 gttttatgga gaattatatc ggctcttcct cgtgtaggca agatatattc ctgtaaattt
     6001 ttcaaattca catttttcca tacatctttt gccattttaa aggcttcttc acgatctccg
     6061 atagcaaaac gataataata attctctgga ttttggaaag ccgtatccag taacgcacca
     6121 aaacgatcca aataccattt ttcgatcagc acgggatcgg catcgacata gacagaaaaa
     6181 tcaaaaaagt cgctgacgta gatttgctga ttcgcaggta attgaagcgt attgatcccc
     6241 tcaacgatca agatatcagg ctgctggatc aattcgtact tgtctggaat gatatcatac
     6301 acatcatgtg agtaaacagg tacttttatc tcttcctgac cagattttac acggttcaag
     6361 aaatcgatca gcatctccat atcataactt tcaggaaatc ctttacggtc catgattcca
     6421 cgctctttta atatacgatt tggataaaga aatccgtcag tcgtgatcag ctgaacgttt
     6481 cttcgtttga aggtacgaga caaaatcgtc tgcagcagtc tggctgttgt actttttcca
     6541 acggctacac ttccggcgat cccgataata aaaggcggta cttctacgta tttatgaaga
     6601 aataaacctt tactcaagaa caaagattca aattctttca tataaagata aatcagatga
     6661 gcaagaggta catagatatc tcgaacatcc tgcatggaaa tgcggtcatt caaacttttg
     6721 atattatcta gttcatcttg tgtcaaaggt gcttgcccat tacgataaaa ttctcgccat
     6781 tcttctcttg ccacacggta gtagttcatt ggatcattca tattagcctc ctgcgttctc
     6841 ccatcaatgg aactatttta acacaaaata aacgcaaacc ccaccactta agagagattg
     6901 ccgatctttt tgtataatct ttataataga aagaaacgga gtcttattct tgacttgcgc
     6961 acggatacat aaaaggatag actgttgtca tgaaagaaat gagaaggatg gaagacatga
     7021 caaatcatta ctattctgaa cgcccagata cggcgcacga ttttgaacaa tgggcttttg
     7081 agttaaaagg cagaaccttt caatttgtaa cagattctgg tgtattttca cgtgaaacag
     7141 tggattatgg ttcacgtgtc ctgattgatg ctttcgattg ggaagagtta cctgatgaag
     7201 gaaagatctt agatgtggga tgcgggtatg gaccgattgg gttagctttg gcatttgcga
     7261 cacagcggtt tgttgaaatg gtcgacatta atgaacgagc ggtaagtcta gcgcaaggaa
     7321 acgcaaagcg taaccaaatc gaacatgttg atatccatca atcgaatatc tatgaagcag
     7381 ttcatgaaga aacctatgca gctatcgtaa gcaatccgcc gatccgtgct ggaaaaaaag
     7441 ttgtacatga aattttgaca ggcgcatatc ctcggttgaa gaagggcggt acactgacaa
     7501 ttgtgatcca gaagaaacaa ggagcgccga gtgcgcaaaa gaaaatggaa gaaacatttg
     7561 gtaatgcaga aatcgtcaca aaagataaag gatattatat catcaaaagt gtgaaagaat
     7621 aactagctga aaaatagtgg agcgacgaaa gagtggaact gggcgtgcat gtacgggtac
     7681 gtatcagttc tttttttgtt gagaagtttt taaaaacaaa gaactatttt ttcttcaaaa
     7741 agaaatagta ggaacagagt cgttgttttt tacgataaaa taaataagat acagaacttg
     7801 tatagaaggc tgccaagtcg gtagataaag gaggttttta tcaaaaaaca tactttcctt
     7861 ttttctctat tcttttcttg ggggttttgg tacactgata gggaatttag atagaaggga
     7921 gccaatagga tgaagctgac catcagagat atcgcagaaa tggcaggcgt atcagtaaca
     7981 actgtctcac agattttgaa taacaaagga agcagattta gtgataagac aagaaaaaaa
     8041 gtgttgtcga tcgtgaatga acatcattat aagcccgatt atttcgcttc aaatgtgatc
     8101 aatcgacatt caaagaccat cggaatgatc gtccctgatg tcactgattt cttcttctcc
     8161 aaactgatcg aaggtgtaga gagctatttg aatcctttgg gctatgtgat catgttatgc
     8221 aattctcgtc atagccaaga aaatgagatc aagtatttac aagagctttc ccaccgctca
     8281 gtggacggaa tcttgctggc cacaccaaat attttacctg agaaatatgc attagacagc
     8341 ggtttttatc aaaaaatgcc aatcatttta atcgatcgcg gattgaatcg tcgggataac
     8401 ggacggctta tcgtaaagga atatgagggc gcctatcaag ctgtttcgct gtttattgaa
     8461 aatggccata cgaagatcgg catgctgaag gaaaccactg gatactatca attagaagaa
     8521 cgtttcaacg gatatcgtca tgcgctgaaa gatcatggcc tgccttttaa tgggaaattt
     8581 gttgaacatg gagagttgac tgtccaaggt ggatatactg catcaaaaaa attactcaaa
     8641 cataaagata tcacggctat tttttgcggg aatgatgcca tggcgatcgg atgctatcag
     8701 gcgatcgatg aattgggcaa aaaaatccca gaagatatct cagtcatagg attcgatgga
     8761 ttaaagctgt cagaatacat gattccgcaa ttgacgacag tgcagcagcc aagttttgat
     8821 attggatttt atgctgcacg tttcctgatc gatacgatcg aatttcctca aagaaaagtt
     8881 ccgaacaaag tgttcgaaac aaaattaatt attcgtgaga gcgtaaaagt attgacaaag
     8941 ggctaactcg gcggtatatt gcttatgtaa gcatttacaa aggctagtta gtgctttttt
     9001 tatttttagt aaaccagttt actaaagtgg tttacctttc ctaaaaataa tgtataatac
     9061 cgaatgaaga caaggaggat caggcaatgc gaatggtgga tctgattgaa aaaaagaggg
     9121 atggaaacga gctgtcgaaa gaggaaatcg agtacatcgt cacgaactat acgaatggaa
     9181 agattccaga ttatcaagtc agtgcattat tgatggcaat tttttatcaa gacatgacaa
     9241 atgaagaaat cacgaactta acattggcga ttgctaattc tggtgatgtc atcgatttga
     9301 gttctcttga aggaataaag gtagataagc attcgacagg tggcgttggt gacacgacca
     9361 cattgatttt agccccccta gtagctagtg tcggagtgac agtggctaaa atgtctggac
     9421 gcggtctagg ttacacaggt ggtacactag acaagctaga agcaattccc ggatttcaga
     9481 tcgagctttc ggatgaagaa tttgtacgca ttgttaatga aagtaaagta gcggtaattg
     9541 gtcaatcagg aaacttggca ccggcggaca aaaaattgta tgccttgcgt gacgtcacag
     9601 cgaccgttga ttctcttccg ctgattgcca gttcgattat gagtaaaaaa atcgcggcag
     9661 gagcagatgc aatcgttttg gatgtgacca ctggagacgg tgctttcatg aagaacatcg
     9721 aagatgcaag acgtctggca aagaccatga cgagcattgg gaaactggct aaccgagaaa
     9781 cagtggcggt tatctcagat atgtcagaac ctcttggcga agctatcgga aatagtctag
     9841 aagttgtcga ggcaatcgaa acattacaag gaaatggtcc agaagatcta gtagaaatgt
     9901 gctacgcctt aggaagccaa atggttgttc ttgctggcaa agctaagaca accgatgaag
     9961 caagaacgtt gcttcaagaa gctctggaat caggtaaagc gttagcaaaa ttcaaagaaa
    10021 tgatccaaaa tcaaggggga gacccagcga tcgtggaaca tccagaacga attttgactg
    10081 cacgctacac aatggagctt cctgctaaac aaagcggagt tgtgtcaaaa atcgtggcaa
    10141 atgaattagg aattgctgca atgatgctag gtgctgggcg taaaaccaaa gaagaagata
    10201 ttgatcacgc tgttggattg aaactgcata aaaaaattgg tgacactgtg acaaaaggtg
    10261 agtcactatt gacgatctat agtaacgacg aagaaatcac ttctgtcatt gaattattat
    10321 ataaaaatat tgaaattggt gaatcggcaa tgaagccaac gttgattcat gacatcatca
    10381 cagaataaag gaaaaaagcg gactttaccg ttttaaagaa aaggggaaaa gaaatggaac
    10441 taaaccgaat gatcgaccat actattttga aagcagacgc atctaaagaa gctgtgatgc
    10501 aaattatcga agaagcaaaa aaataccatt tttattctgt ctgcatcaat ccagcatggg
    10561 ttgcgctagc gaaagaacaa ttacaaggtg agccagtagc cgtatgtaca gtaatcgggt
    10621 ttcctctagg agcaaacaca cccgagacaa aagcatttga aacaacagat gccatcaata
    10681 acggtgcaga tgaagtcgat atggtcatca atatcggtgc tttgaaatca aaggatgatc
    10741 aacaagtcca aaaagatatc gaagcagttg ttgaagcagc aaaagatcgt gcgcttgtca
    10801 aagttattat tgaaacatcg ttattagata gagaagaaat catccgagct tgcgaaatag
    10861 caaaagctgc aggcgctgat tttgtcaaaa catcaactgg tttctcaaca ggcggagcaa
    10921 aggtagagga tgtccgtttg atgcgtgaaa ctgtaggacc agaaatgggt gtcaaagctt
    10981 ccggcggtat ccataacgaa gaagaagcaa tggcaatgat cgaagctggt gctacaagaa
    11041 tcggaacaag cgcaggagta gcaatcgtat ctggttcaac tggtgaaggc tattaatttt
    11101 ttcaaaggag aagctcatgg cagaactcaa acaagaatgg atcaacacag ctattgaagc
    11161 gttagataaa gcctatgttc cttattccca ttttccggta ggcgcttgtc tcgtaacgga
    11221 atcaggcaag atttatcaag gcatcaacat tgagaatgca tcatttggat taacgaattg
    11281 tgccgagcgc acagcttttt tcaaagcagt atctgaagga gaaaaaacat ttacgcatct
    11341 agtcgtagcg gggcacaccc ccgatcctat ctctccttgt ggtgcatgtc gacaagtaat
    11401 ggcagaattc tgttcccctg atatgccggt tacgctagta ggagacaatg gtgtgacgaa
    11461 ggcaacgacc gttcgtgcac tgctgcctta tgcatttact gaaaaagatc tataactcat
    11521 gttcattcag tcagctgact gttgaaaaat aaaacaatta ttttaggagg ctttacagaa
    11581 atgaaaaaag caaaattatt tggtttaggt gctgtcgcac tagcagcagg gttattttta
    11641 ggtgcatgcg gcaacaacgg ctctactgat tcatccggtg gcaaaagctc atcagacaca
    11701 acaactgcag cattgatcac agacacaggt ggtgtagacg accgttcatt caaccagtca
    11761 gcttgggaag gactagaaaa atgggggaaa gatcaaggac tttcacgagg aaatgatggc
    11821 ttccaatatt tccaatcttc aaatgaatca gattacatcc caaatattga ccaagcattg
    11881 aatgctggat tcaaaacgat ttttggtatt ggttataaac taaaaccagc gatccaagaa
    11941 caagcaacaa acaatacagg tacgaacttt gttattatcg atgatgtcat cgatggatta
    12001 gataacgttg tatctgcaac tttcaaagac aacgaagctt cttatcttgc aggtgttgca
    12061 gcagcttata cgactgaaac aaacgtagtc ggctttatcg gcggtgtaaa aggtgaagta
    12121 atcgatcgtt ttgatgctgg attcaaagcc ggtgttgatg caggtgcgaa agaattagga
    12181 aaagaaatca aagtattgaa tcaatatgct ggtgacttct ctgctccaga taaaggacgt
    12241 tcaattgctc aaggtatgta tgcacaaaac gcagatatca tcttccatgc ttcaggcggt
    12301 acaggtaacg gtgtattcca agaagcaaaa tcattgaacg aatcaggcga caaaaaagtt
    12361 tgggttatcg gtgttgaccg cgaccaatca gacgaaggcg aatacacatt aaacggcgaa
    12421 aagaaaaact tcacattgac atctacactg aaagcagttg gaacagtagt agaagatttg
    12481 gcacaaaaat cagcggacgg taaattccct ggcggcgaac acactgttta tggattgaaa
    12541 gaagacggtg ttggtctaac agaaggacaa ctttctgacg aagcgaaaaa agcagttgat
    12601 gaagcaaaag aaaaaatcat ttcaggtgac gtaaaagttc cagaaactcc tgaagaaaac
    12661 taaaaccatt tctatagcct tgttagacac atgtttgaca aggctatggt ttgattagaa
    12721 caagaattgt tcatttatcc aagctgcctt gtgacagttt ggataaggga acaactccct
    12781 gaagtaatgt ttgtttcaaa agaagaacgc tcactaagcg tgaatcttag tcaatcgttg
    12841 ccctagatag aaatcgttag gatgtgaaaa aaaggtggtt caagaaaata ttgtcataga
    12901 aatgcagaat attaccaaag aatttggaac attcaaggca aatgacaaca tcaatttaca
    12961 agtaaaggca ggagaaattc atgcgttgct tggagaaaac ggtgctggca aatctacatt
    13021 gatgaacgtg ctttccggat tattagagcc gacatcaggg aaaattttga tgcgtgggaa
    13081 agaagtaaag atcacaagtc cgaccaaagc caaccaatta gggattggga tggtccatca
    13141 gcactttatg cttgttgatg cctttactgt aacagaaaac atcgtgttgg gaagcgagcc
    13201 tagtcgcgca gggatgcttg accataaaaa agcacgaaaa gagatccaaa aagtttctga
    13261 acagtatgga ttgtcagtca acccagatgc ttacgttcgt gatatttcag ttggaatgga
    13321 acaacgggta gaaattttaa aaacacttta ccgaggagca gatgtactga tttttgatga
    13381 gccgacagct gtattgaccc ctcaagaaat tgatgaatta atcgtgatca tgaaggaatt
    13441 agtcaaagaa ggcaagtcga tcattttgat tacgcataag ttagatgaaa tcaaagcagt
    13501 agctgaccgc tgtacagtta tccgccgtgg aaaaggaatc ggtacagtca acgttaaaga
    13561 cgtaacctca caacaattag ccgatatgat ggttggaaga acggtttcat tcaaaacgat
    13621 gaaaaaagaa gcgaagcctc aagaagtcgt tttgtctatt gaaaatctag tggtgaaaga
    13681 aaatcgtgga ttagaagccg tgaaaaactt gaacttagag gttcgtgctg gcgaagtact
    13741 tggtatcgct ggaatcgatg gaaacgggca gtcggagttg atccaagctt tgactggttt
    13801 gcgaaaggca gaaagcggac atatcaagct aaaaggggaa gacatcacta ataaaaaacc
    13861 ccgaaaaatc actgaacatg gtgtaggaca cgtgccagaa gaccgtcata aatacgggtt
    13921 agtcctagat atgacattat ctgaaaatat cgcactacaa acctatcatc aaaaacctta
    13981 cagtaaaaac ggcatgctga attattcagt aatgaatgaa catgccagag aattgatcga
    14041 agaatatgat gttcgaacaa cgaatgaact tgttcccgca aaagctttat caggcggaaa
    14101 tcagcaaaaa gcaattatcg ctcgtgaagt cgaccgagat cctgatctgt tgattgttgc
    14161 aaatccaact cgtggactgg atgtaggtgc gatcgaattt attcataaac gtctgatcga
    14221 acaaagggac aaatacaaag cagtgttact gatcagtttc gaattagaag aaattttaaa
    14281 tgtttcggat cgtattgctg ttatccatga aggagaaatt gtcggtatcg ttgatccgaa
    14341 agaaacatct gaaaatgaat taggtctatt gatggcgggc tataccttag aagaagcacg
    14401 aaaagagtta gaaaaggggg ctggcgcaca tgaatgatcg cagcgaaaaa ttccgtaatt
    14461 tattagtgcc tgtactttca gttgttttag gattgatctt aggcgcgata ttgatggctg
    14521 cttttggttt tcagcccatc caaggttatt cttctatgct agatgcagcc cttggttcgc
    14581 aaagagggat aggtgaaacg ttaaggcaag caacgccgtt gatttttaca gcattaggtt
    14641 tttctgtagc taactcagct gggtttttca atattgggct ttctggacaa gcactttgcg
    14701 ggtggatcgt cagtgtctgg gtggcacttg cgtttccaga tctgccaaaa gttgttttgc
    14761 ttccgttatg cgtgatctta ggtgccttag ctggtgcgat ggcagcggct gttccaggtt
    14821 tacttcgagc attttttgga acaagtgaag tgatcgtaac aatcatgatg aactatatct
    14881 tattatacgt aagcacattt gctttacatg aagtgatgcc tgaaagctat cgctcaagtt
    14941 tggactcttc caatatgatc agtcaaaatg cctcactgcg gttaccatgg ttaacacaag
    15001 cttttggcgg ctctcgcgta aactcaggat tatttttagc aatcatcgcg cttattttcg
    15061 tttggctgct gatgaagaaa acgactttag gatttgaaat ccgttctgtt ggattgaatc
    15121 cttttgcttc tgagtatgca ggaatgagta gtaaacgtac gatcgttttg tcgatggtca
    15181 tttctggcgc tttggcaggg cttggcggtg tggttgaagg attaggaacg tatcaaaact
    15241 tctttgttca aacgacttcg ctatctattg gatttgacgg aatggctgtt tccttgcttg
    15301 gttctggatc agcaatcggt attcttttat cagcgatctt gttcagtatt ttgaagatcg
    15361 gtggacttgg catgcagaca ggagcaggtg ttccgtttga gatcgtcaat gtatcgattg
    15421 ctttgattat cttctttgtc gggatcaact tcctgattcg tttcgtcatg gctaaagtct
    15481 tccaagggaa aaaacaagaa gagatcgttg cgacaataga atcgaaacca acgaatcaaa
    15541 accatgaagg gggcgaacta taatgtcgac tatcgaatta attgccttga tccttacttc
    15601 tacactggtt tattcaacac cgctaattct aacgtcacta ggcggaactt tttctgaacg
    15661 aagcgggatc gtaaacgtag ggctagaggg aatcatggtc atgggtgctt ttagttccat
    15721 cgttttcaac ttgacgatga gtccatcact tggttctatg acaccatgga tcgctatttt
    15781 agtgggtggc gtcgtaggta tcctattttc tctgattcac gcagtagcaa cagtcagttt
    15841 gcgtgctgac catattatca gcgggacagt aattaatctg atggcacctg ctttaggtgt
    15901 tttcttgatc aaagcatggt atggtaaagg tcaaactgat aacatcacgc agaatctggg
    15961 gtatttttct ttcccaggat tagcagatat tccggtcatt ggaccaattt tctttaaaaa
    16021 tacattctta cctgcttatt gtgctatttt gattgctgtc ctagcttggg tgatcatctt
    16081 caagacgaaa ttcggcttgc gcctccgttc agttggtgaa aatcctcaag ctgccgacac
    16141 attaggtatc aatgtatatg ctatgcgtta ttcaggcgta ctgatttctg gcttgttagg
    16201 cggtatgggc ggtgctgttt ttgctcagtc gatttccggt aacttttcag ctggtacgat
    16261 cgttggacaa gggttcatct ccatggctgc tatgatcttt ggtaagtgga atcctttagg
    16321 ggcaatgggt gcctcattgt tctttggttt tgctcaaagc ttgagtatca ttggcgcaca
    16381 attaccagtt atttcttcga ttccgccagt attgctgcaa attgctccat atttgctgac
    16441 aatcattgtg ctggttgttt tcttagggaa agcttctggt ccaaaagcaa acgggaagaa
    16501 ctatatcaaa tcaaaataat cttttagcga ataaaaaggg tgggttacgg gaagtgtttt
    16561 tcgtctcgcc ctttttgttt taaaggcaat aaaaataaaa agaaagaagg aataatcatg
    16621 tttaaacgcg tacatctgat cgtcatggat tcagtaggaa ttggagaagc accagatgcc
    16681 gataagttcg gtgatgttgg ttctgataca ttaggacaca ttgcgaaaga agcaggattg
    16741 aacgttccac atttagagca gcttggttta ggaacgatcc gtcctcttga aggtgtggaa
    16801 tcagtgaaag atcatcaagg ctatgcgacg aaattagaag aagtttctgt agggaaagat
    16861 acgatgacag gccactggga aatcatggga ttgaatatca aaactccatt tcgtgtgttt
    16921 cctaatggat tccctgatga tctgatcaaa caaatcgaag aattttctgg ccgtaaagtc
    16981 gtatgtaaca aaccttatag cggaacagct gttattgacg attacggtga acaccaaatg
    17041 aaaacaggcg atttaattgt atatacttct gccgatcctg tccttcagat cgcggcacat
    17101 gaagaaatca ttcctttaga agaattatat cggatttgcc aattcgttcg tgatataaca
    17161 aaagatgatc cgtatatgat tggccgaatc attgcacgtc cgtacgtggg tgaaccagga
    17221 gacttcacac gaacaagcaa tcgccatgat tatgcgttga atccattcgg acacacagtc
    17281 cttgattctt tgaaagaagc aggaaaggac gtgattgctg ttggaaagat caacgatatt
    17341 ttcaacggac aaggaatcac tgaatctgtc cgcacgaaaa gcaatatgga tggcgttgac
    17401 cagctgctga aagttatgaa aaacgatttt tcaggaatca gctttaccaa tttagtagat
    17461 tttgatgcat tatacggtca tcgtcgggat gtagtcggct atgcgcatgc gattgaagaa
    17521 tttgacttac gaattccaga attgcttgaa gcgatggaag aagacgactt gctaatgatt
    17581 acagccgatc acggaaacga tccgacgttt actggtaccg atcatacgcg agaatatgta
    17641 ccgttattgg tttacagtaa aaaaatgaaa gaacaagggg cattgcctca aggatattat
    17701 tcagatattt cagcaacgat tgctgagaac tttagtgttt cttcgactga aaatggcgaa
    17761 agcttcttga aattattaaa ataatctttt taaataggag aaagcaaatg accaagttaa
    17821 gcgagatgtt acaagaaacg acccagttta tccttgataa aggagtaaaa gaaattgatt
    17881 ttggtttgat tctaggttca ggattaggtg aattagcaga tgaaatcgaa gatgctgtaa
    17941 tgatttctta tgatgagatc ccgttctttc ctgtttcgac agtagttggg catgcaggaa
    18001 agctagttta tgggtctctg gcaggcaaga aagtgttagc catgcaaggg agattccact
    18061 actatgaagg tcattccatg caaacagtga cctatccagt acgtgtgatg gccggactag
    18121 gggcccattc agtcattgtg actaatgcat gtggtggtgt caacgaatca tttgtaccag
    18181 gagatttgat gctgattacc gatcatatca attttactgg tgaaaatcct ttgatcggtc
    18241 cgaatgaaga tgcaatgggc cctagatttc cagatatgag tcaagcttat acgcctgact
    18301 atcaagcaac agcaaaagca gcagcagaaa aactaggttt ggatcttaaa gaaggcgtat
    18361 atatgggcta ttctggacca acttatgaaa cgcctgcgga gatccgaatg tctcggacaa
    18421 taggagccga tgcagtaggg atgtcgactg ttccagaagt catcgtagca gctcacagcg
    18481 gattaaaagt gttaggaatc tcttgtatta ccaacttagc agcaggtatg caagcgaatc
    18541 tgagccatga agaagttgta gaaacaactc agagagtcaa acaatctttt aaagcattgg
    18601 ttaaagaaac attgacatta ttgtaatagg aggaacaata aaatgagtgt gcatattgaa
    18661 gcgaaagaag gcgagatcgc cgataagatt ttacttccgg gagatccttt gcgagctaaa
    18721 tatattgcgg agactttttt ggaagatcca gtttgttaca atcaggtacg cggtatgtta
    18781 ggctttaccg gcaaatataa aggagaacgt atttctgttc aaggaacagg aatgggaatg
    18841 ccatcagcca ccatctatgc ccatgaattg atccagtcgt atggtgtaaa gaaattgatt
    18901 cgagtaggta catgcggtgc attgtcaaaa gacgtccatg ttcgtgattt agtactagca
    18961 caaggtgcag cgaccagttc ttcgatgatc gaaaaaaatt tccaagcctt tcattttcct
    19021 ccaatcagtg atttcaatct attactgaaa gcatatgaga tagctaaaga aaagggctat
    19081 acggttcatg taggaaatgt tctttcggaa gattctttct acaaagatga tttgacagag
    19141 acgttccaat tagctgaact tggcgtttta ggagtggaaa tggaagcagc ggctctttat
    19201 tacttaggcg cgaaatacca tgtgcagaca ttaagtttga tgaccgttag cgaccatttg
    19261 atcaccggtg aagaaacaac agctgcagaa cgtcaatcta cgttcaatga aatgatcgaa
    19321 gtaggattgg aaacagcgat agcagaataa gaaggaaatg aaaaagctgc cctgcattaa
    19381 gtaataagaa catccaagca aaaatagctc ctcatgtttt tgacttggct ggacttatta
    19441 ttgcaaatgt aagcttttga acaccgttta ccaagaggtt gtgaatctgc cgtttaactc
    19501 tgagtcacaa ggaacaattt caaaaaacag cttttcatgt ttgatgaata ttgtgacttg
    19561 tgaccgagaa gttggcagga gaaaaccgtt tatacgaaaa aagggactgt gacaaatcgt
    19621 gtcacagtcc cttttttata gtgtttttta taggtagtat tttttagcaa ctgttaagtc
    19681 atcatttaat tcataaacaa gtggttgacc tgttggaatt tcaagatcca tgatgtcttc
    19741 gtcagagata ccttcgatat gtttagctag ggcgcgtaat gagttaccat gtgcagctac
    19801 taaaacagtt ttgttgtcta gtaatgctgg agcaatttca tcttgccaga atggcaaagc
    19861 gcgttctaat gtcactttca agttttctcc accaggaata tcgcgtttgt ctaacattgc
    19921 gtaacgacgg tcatttgctg cagaaccttc gtcagtttct tccattaatg gaggaagagt
    19981 atcgtaagaa cgacgccaga tgtgcacttg ttcgtcgccg tatttttcag ctgtttcttt
    20041 tttgttcaaa ccttgtaatt taccatagtg acgttcgttt aagcgccatg atttgatttg
    20101 tggtacccaa agttgatctg aatattctaa gatcaagtta catgttttga ttgcacgagt
    20161 taaaacagaa gtgtaagcaa catcaaattc gattccagct tctttgattt tgcgtccgcc
    20221 ttcttttgct tcttcgattc cttctggtgc tagatttaca tcagcccaac cagtaaattg
    20281 gttcaatgca ttccattcac taagaccgtg acgagaaaaa actaattttg gcatgtttag
    20341 ctctcctttt aagataggtt tatatttctg aaattttcag ttctgtttta ttgtacaatt
    20401 ttttggaaga gatttccatc gaaaaattag ataagattct atgagaaaac aagtagagaa
    20461 tataaaaaaa cgaacagtgc ccacgcaact gttcgttttt aggtattaga tgcataattg
    20521 ctcgatttct ttttcaggta aattgatata tttataacat gttccaactg aaacaccgca
    20581 ttttgaagaa ataaattgga tcgtttcttt tttttcataa tagagacgtc ggattttttt
    20641 gatcgttttt gcatcgattt ttggtcttcc gccgattttt ccttttttac gagcttcatc
    20701 taatccaact aatgtacgtt ctttgatcaa tgcgcactcc atgtttgcta ggttttccat
    20761 taattggaaa tagatttttc ccatttcgcg cgtatcgatt ccttcatcaa gactaatcaa
    20821 gtgcagcccg cgatctttga ataattgcgt gagttctgtt aattgccgcg tagatttgcc
    20881 taatcgatga agttggcaaa tgactagtgc atcgccttgt tgcatgtcag cgatcactga
    20941 ctccaaatga acagcagtag aatcattttg ttccacttca tatgtctcat ggaaaatttc
    21001 ttgacaacca aagtcagata attgatcaac ctgagttgtt aaatcaacgt catttatggt
    21061 tgtgcgtgca taaccaataa ttttcatggt gattcctttc tagcagtgat tttctaactg
    21121 gatataacag taaaaatgta gcataaagta cgtcaaaaag atagtttttt cgcaaatttt
    21181 ccgctgtctt tttttattgg atgatcaagc agtttttctt tatttacgac ggattgcgtc
    21241 aagaatctca acgccttttt ctgagccgac aactacttta gaaaccatgt ttaggaaaag
    21301 cccatgttct actaccccaa caagttcatc taaataggta gctaagcttt ctggctgttc
    21361 gatgacttct agatgcagat caatgatgta atgaccacca tctgtggtta agacttctcc
    21421 gttttcgtct gtacgcaaga cgggttggaa tcttttttct tcaaaaatat gaagtagctg
    21481 ttgggagcca taagggatca cttcaactgg gagtgggaac ttcccaagtt tatcgactaa
    21541 tttagaagaa tcaacgatcc aaatcgtttc ttttgagtac gtagcgacta ttttctcgaa
    21601 aagcaaagca gcgccgccgc ctttgatccc ttgaaaatca gcactgattt catcagcacc
    21661 gtctattgtc aaatcgacta cggggacttc atcaatactc tttaacggta ttcctaattt
    21721 ttccgcttgt tcttttgtcg catttgaagt agtaactcct gtgatacgta aaccttcttc
    21781 acgcatacgg cgaccaatct cttctaccat ataataggcg gttgaaccgg tcccgagacc
    21841 tacgatcatc ccgtccttta cgaatttcgc ggcttcaatg ccagctaatt gttttaaatt
    21901 catctttgcg tccacctttt tttagtttga gcatcgtgct ttcctcacta ttttagcgtg
    21961 atttacggta aaaagatacc attctttttt attccaataa agctttttca aaaaaaattg
    22021 catctcttgt tgacatgttt tttcattcgt gatattctaa caaaggtgct ttatctacag
    22081 gtttctgtga agaaacgtgc tgactgttta tcaaagcata tttgataagg atgcagacat
    22141 tgcatttatt ttttatcaca aaaaaagaac cacctggatg tgtggaacta gacgcaaatc
    22201 gtgggaagga ggaaacaagg aatgcctaca attaaccaat tagtacgtaa acctcgtaaa
    22261 tcaaaggtgg aaaaatctaa ttcacctgct ttgaacaaag gatataacag ctttaagaaa
    22321 actcaaacta acgtaaactc acctcaaaaa cgtggggtag caactcgtgt gggtacaatg
    22381 acacctaaaa aaccgaactc agctttacgt aaatatgcac gtgttcgttt gtcaaactta
    22441 attgaagtta ctgcttatat cccagggatt ggtcataact tacaagaaca cagtgttgta
    22501 ttattacgcg gtggtcgtgt aaaagactta ccaggggtac gttaccatat cgttcgcggt
    22561 gctttagata ctgctggtgt taacgaccgt aaacaaagcc gttctaaata cggaactaaa
    22621 aaacctaaag cttaatttta attattaaac aaatgaaact tgcttctact atatagaagt
    22681 aattactatg aacaatattt cggaaggagg agttacggat gccacgtaaa ggtcctgttg
    22741 caaaacgtga tgttttacca gatcctattt ataactcaaa attagtaact cgcttgatca
    22801 accgtgtaat ggttgacgga aaacgcggga ttgcagctaa cattatctat aatgcctttg
    22861 acttgatcaa agaatctaca ggtaacgatc cattagaagt gtttgaacaa gcaatgaaaa
    22921 acgttcaacc tgttcttgaa gttaaagcac gtcgtgttgg gggttcaaac taccaagtac
    22981 cagttgaagt tcgccctgaa cgccgcgtta ctctaggtct tcgttgggta gtaaactacg
    23041 ctcgcttgcg cggtgaacac acaatggaag aacgcttagc aaaagaaatc atggatgctg
    23101 caaacaacac aggtgcttct gttaaaaaac gtgaagacac tcataaaatg gctgatgcca
    23161 accgtgcgtt tgctcactat cgctggtaag attctctctg tttggtcggc ttttataagt
    23221 cgacctcaga tagtgtcctt atttttagca actagatagt aaagagagga gaaacaagga
    23281 gaatgaccag agaattttct ttacaaaaca ctcgtaatat cggtatcatg gcccacatcg
    23341 atgctggtaa aacgactgct actgaacgta tcttgtacta tactggtaaa atccataaaa
    23401 ttggtgaaac tcatgaagga gcttcacaaa tggactggat ggaacaagaa caagaacgtg
    23461 gtattacgat cacttctgct gctactacag cacaatggaa gggtcaccga atcaacatca
    23521 tcgacacacc aggacacgta gacttcacag ttgaagttga acgttcattg cgtgtacttg
    23581 atggtgctgt aactgttctt gatgcacaat caggtgttga acctcaaact gaaacagttt
    23641 ggcgtcaagc aacaacttat ggtgtacctc gtatcgtatt tgcaaacaaa atggacaaga
    23701 tcggtgctga cttcttatat tcagtatcaa ctttacatga ccgtttgcaa gcaaacgctc
    23761 acccaatcca attaccaatt ggtgcggaag acgacttcac aggaattatc gaccttgtaa
    23821 caatgaaggc tgaaatgtat acaaacgatc ttggaacaga gatcgaagaa acagaaattc
    23881 ctgaagaata ccgcgaatta gctgaagaat ggcgcgaaaa attggttgaa gctgtagctg
    23941 aaactgatga agaacttact ttgaaatact tagaaggtga agaaatcact gaagctgagt
    24001 taaaagaagg tatccgccgc gcaacagtta acgtagaatt ctacccagta ctatgtggtt
    24061 cagcatttaa gaacaaaggg gttcaattac tattggacgc tgttcttgat tacttgccat
    24121 caccgttaga tatcccagct atcaaaggga ttgaccctaa aactgatgaa gaagtagaac
    24181 gtcctgcaga cgacagcgct cctttctcag ctttagcatt taaagttatg actgacccat
    24241 tcgtaggtcg tttaactttc ttccgtgtgt actctggtgt tttacaatca ggttcatacg
    24301 tacaaaacgc aacaaaaggc aaacgtgaac gtgttggtcg tatcctacag atgcatgcca
    24361 actctcgttc agaaatctct gaagtttacg ctggagatat cgctgctgct gttggtttga
    24421 aagacacaac tactggggac acactatgtg atgaaaagag tctagttatt cttgaatcca
    24481 tggaattccc agaaccagtt atccaagtcg ctattgaacc taaatcaaaa gctgaccaag
    24541 ataaaatggg tgtggcttta caaaaactat ctgaagaaga tcctactttc cgtgctgaaa
    24601 caaacgtgga aacaggcgaa acaatcatcg ctggtatggg tgaacttcac ttagatatca
    24661 tcgttgaccg tatgcgtcgt gaattcaaag ttgaagctaa cgtaggtgcg cctcaagtat
    24721 cttaccgtga aactttccgt gctggcactc aagccgaagg taagtttgta cgtcagtctg
    24781 gtggtaaagg tcaatacggt cacgtatgga ttgaatttac accaaatgaa gaaggtgctg
    24841 gtttcgaatt tgaaaatgcg atcgtcggtg gggttgttcc tcgtgaatac attccagctg
    24901 ttgaaactgg tttgaaagat gctatggaaa atggtgtctt agctggctat ccattagtag
    24961 atatcaaagc taaactttac gatggttcat accatgatgt cgattcaaat gaaactgcct
    25021 tccgtgttgc tgcttctatg gcattacgtg cagcagctaa gaaagctaat ccagtaatcc
    25081 ttgaaccaat catggctgta gaagttgtta tccctgaaga ttatttaggt gatgttatgg
    25141 gtcacgtaac tgctcgtcgt ggacgtgttg aaggtatgga agctcgcgct ggcggccaac
    25201 aagttgttcg tgcgatggtt ccacttgctg aaatgtttgg ttatgctaca actcttcgtt
    25261 ctgctactca aggtcgtggt acatttacaa tgacatttga ccactacgaa gatgtaccaa
    25321 aatcagtaca agaagaaatc attaagaaaa acggcggaaa agctgaataa tttcttaaaa
    25381 ccggatagtc ctgcaaagag attatccaaa gagactatat ttttaatata gtttcgtgta
    25441 aaataattat cgatgatatg ggcatgtggt gtaccttgtg tacatcatat acctatcaga
    25501 caaataaaaa atatattgta tttttaggag gacattttaa aatggcaaaa gaaaaatttg
    25561 accgttctaa accacacgtt aacattggta ctatcggaca cgttgaccat ggtaaaacta
    25621 cattaacagc tgcaatcaca actgtactat ctaagaaaaa cggcggccaa gctatggctt
    25681 acgatcaaat cgatggtgct ccagaagaac gcgaacgcgg aatcacaatc tcaacagctc
    25741 acgttgagta tgaaacagat acacgccact acgctcacgt tgactgccca ggacacgcgg
    25801 attacgttaa aaacatgatc actggtgctg ctcaaatgga cggagctatc ttggtagttt
    25861 ctgctgctga cggcccaatg cctcaaactc gtgaacacat cctattgtct cgtcaagttg
    25921 gtgttcctta catcgttgta ttcttgaaca aagtagacat ggttgatgac gaagaattac
    25981 tagaattagt tgaaatggaa gttcgtgacc tattaacaga atacgaattc cctggtgacg
    26041 atgttcctgt agttgctgga tcagctttga aagctctaga aggcgacgct tcatacgaag
    26101 aaaaaattct tgaattaatg gctgcagttg acgaatacat cccaactcca gaacgtgaca
    26161 acgacaaacc attcatgatg ccagttgaag acgtgttctc aattactgga cgtggtactg
    26221 ttgctacagg tcgtgttgaa cgtggacaag ttcgcgttgg tgacgaagtt gaagttgttg
    26281 gtattgctga agaaacttca aaaacaacag ttactggtgt tgaaatgttc cgtaaattgt
    26341 tagactacgc tgaagctgga gacaacattg gtgctttact acgtggtgtt gcacgtgaag
    26401 acatccaacg tggacaagtt ttagctaaac caggtacaat cacacctcat acaaaattct
    26461 ctgcagaagt atacgtgttg acaaaagaag aaggtggacg tcatactcca ttcttcacta
    26521 actaccgtcc acaattctac ttccgtacaa ctgacgtaac aggtgttgtt gaattaccag
    26581 aaggaactga aatggttatg cctggcgaca acgtaacaat ggaagttgaa ttaatccacc
    26641 caatcgctat cgaaaacggt actaaattct caatccgtga aggcggacgt acagttggtg
    26701 ccggtgtcgt tacagaaatc aaagcttaat tcacttaagt tttaaagtaa agcaacattt
    26761 attcggacgt aatcgctcgg aacgaaggtc ttaggatctt tgttccgagc ttttttattt
    26821 attgcaggat caggaaaaaa gacaggagta ttataaatag aagaaacacg aaataaaaga
    26881 gtcagtattt tctaaatgaa actttatgaa aacaaaatgt tttttacaag aaaaagcctt
    26941 gctttgtgag tttatatata ttataatgac aaaggtgcgt ttactttagt gaattgcaca
    27001 gacgtaatca gaatgatttc gtcggctaag aaacgagagg ttgcgacacg cccggacgct
    27061 ttgccacgaa cgagcgtgac ggaaattttc gtggagctat gtctactttt caaaataggc
    27121 gaaggaggga acaagatggc aaaacaaaaa attcgtatcc gtttaaaagc gtatgaacac
    27181 cgtattttag atcaatcagc ggataaaatc gtggaaactg caaaaagaac tggagctagc
    27241 gtgtctggtc caattccatt accaactgaa cgcagtcttt acacaatcat ccgtgcgact
    27301 cataaataca aagattcacg cgaacaattc gaaatgcgta cacacaaacg tcttatcgac
    27361 attgtgaacc caacaccaaa aactgttgat gctttgatga agctagactt accatctggt
    27421 gtaaacatcg aaatcaaatt ataaaactaa aacaaaaata atggaggtgt actcatgacc
    27481 aaaggaatct tagggaaaaa agtgggaatg acacaaatct tcactgaatc tggtgaatta
    27541 attccagtaa ctgttgtaga agctactcca aacgtagtac tacaagtgaa aacaatggaa
    27601 acggatggct acgaagctat tcaagttggt taccaagata tgcgtgaagt tttatcaaac
    27661 aaacctgcga aaggtcatgt tgcaaaagca aacacggctc ctaagcgctt cattcgtgaa
    27721 ttcaaaaatg ttgagctagg agaatatgaa gtaggaaaag aaattacagt agatgttttc
    27781 caagcaggag acattattga tgtaacaggt actacgaaag gtaaaggatt ccaaggggtt
    27841 atcaaacgcc acggacaaag ccgcggacca atggctcacg gttctcgtta ccatcgtcgt
    27901 cctgggtcaa tgggtccagt tgcacctaac cgtgtattta aaaacaaacg tcttgctggc
    27961 cgtatgggtg gcaaccgcgt aactatccaa aacttggaag ttgtacgtgt cgatgcagaa
    28021 agaaatgtta tcttgatcaa aggtaatgtt cctggagcta aaaaatcttt aattactatc
    28081 aaatcagctg tgaaagctaa ataattgtag gaagaaagga ggaactaagg aatgccgaat
    28141 gtagcattat tcaaacaaga tggaagccaa aacggtgaaa tcacacttaa cgaagaaatc
    28201 ttcggaattg aacctaatga aagtgttgtt tacgatgcaa tcgtaatgca acgtgcgtca
    28261 ttaagacaag gaacacacgc ggttaaaaac cgtagcgctg ttcgcggcgg cggacgtaaa
    28321 ccatggcgtc aaaaaggaac aggtcgtgca cgtcaaggat ctatccgctc accacaatgg
    28381 cgtggaggtg gcgtagtctt tggaccaaca ccacgttctt acagctacaa acttcctaaa
    28441 aaagttcgtc gtttagcaat gaaatctgta ttgtcagaaa aagttgctga aaacaacttg
    28501 gtagcaatcg aaggcttgaa cttcgacgca ccaaaaacaa aagaattcaa acaagttctt
    28561 gctaacttat ctatcgactc taaagtattg gttgttctag aaaaaggtaa cgactttgct
    28621 gctttatcag cacgtaactt accaaacgtt tctgtagtaa cttctgataa tgtgagtgtg
    28681 ctagacgttg tatcaaatac aaaagtgtta gcaacacaaa ctgctcttac tcaaattgag
    28741 gaggtgcttg cataatgaac ttactagacg taatcaaacg cccagtgatc actgaaaaat
    28801 caatgcttgc catggatgac aagaaataca cttttgaagt ggacactcgc gcaaacaaaa
    28861 ctttagtaaa acaagcggta gaagctgctt ttgacgttaa agtcaaaaac gtgaacatcg
    28921 tgaacgtacg tgcgaaattc aaacgtatgg gcaaatatgc aggatataca aaaaaacgtc
    28981 gcaaagcgat cgttacattg actgaagatt caaaagaaat ccaattattc gaagctgctg
    29041 aataagcact aatagataac tcattaaatt aggagggaaa aacgtggcga ttaaaaagta
    29101 caaacctacc tcaaatggtc gtcgtaacat gacaggttct gattttgctg aaatcacaac
    29161 atcaacacct gaaaaatcat tgttacaacc attgaaaaac catgctggac gtaacaacaa
    29221 cggccgcatc actgtacgtc atcaaggtgg cggacacaaa cgccaatatc gcgtgatcga
    29281 cttcaaacgt aacaaagata acgttgttgc aactgttcaa acgatcgaat atgatccaaa
    29341 ccgttcagct aacatcgcgt tagtacacta tgaagatgga gtgaaagctt acatcttagc
    29401 acctaaagga ctagaagtgg gtatgaaact tgtttcaggt ccagaagcag atatcaaagt
    29461 cggaaatgct cttccattag aaaacattcc agttggtact gttatccaca acattgaaat
    29521 gaaacctggt aaaggtggac aattgatccg ttctgctggt acaagtgctc aagtacttgg
    29581 taaagaaggc aaatacgtat tgatccgctt gaactctggt gaagttcgta tgatcttagc
    29641 tgcatgccgt gctacaatcg gttctgtagg taacgaacaa cacgaattaa tcaacatcgg
    29701 taaagctgga cgctctcgtt ggatgcgtaa acgcccaact gtacgtggta gcgtaatgaa
    29761 ccctaatgat cacccacacg gtggtggtga aggtaaagct cctatcggac gtaaagcacc
    29821 agtttctcct tggggtcaac cagctcttgg ttacaaaact cgtaacaaaa aagctaaatc
    29881 agacaaactt attgttcgtc gtcgcaaaaa taaataatta ctttgaccat gtgtcgcaca
    29941 ttttgagagg aggttcacca tgggtcgtag tttaaagaaa ggaccttttg tcgatgagca
    30001 tttaatgaaa aaagttgaag ctcaagcagg ggttgaaaag aaaaaagtta tcaaaacttg
    30061 gtcacgccgt tcaacagttt tcccaagttt tgttggatat accatcgcag tttatgatgg
    30121 acgtaaacat gtaccagttt acatccaaga agacatggta ggacacaaat taggtgaatt
    30181 tgcacctaca agaacttatc gtggacatgc tgctgacgat aagaaaacga aacgctaatt
    30241 tgaggggagg ataaatccaa atggcagaac aaatcacatc agctaaagca actgcaaaaa
    30301 cagttcgcac ttcacctcga aaagcccgtt tagtaattga ccttatcaga ggtaaaagtg
    30361 tcgcagatgc aatttcaatc ttgaaattca caccaaacaa atctgctgga atcattgaaa
    30421 aagtattgat gtcagcagtt gcgaatgcag aaaataactt tgacttagat gttgaaaact
    30481 tggtagtaag cgaagctttt gttaacgaag gacctacaat gaaacgtttc cgtcctcgtg
    30541 caaaaggatc agcttctcca atcaacaaac gtacaagtca tatcacggta gtcgtatcag
    30601 aaaaataagg agggataatc agtgggtcaa aaagtacatc caattggaat gcgtgtaggc
    30661 atcatccgcg actgggatgc taaatggtat gctgaaaaag agtatgctga gttcttacac
    30721 gaagatttaa gaatccgtaa atttatttca actaaacttg ctgatgctgc tgtatcaaca
    30781 gttgaaatcg aacgcgcaac aggtcgcgta aacatttcaa ttcacacagc taaaccaggt
    30841 atggttattg gtaaaggcgg ttctgaagtc gaaagcctaa gaaaagaatt aaacaaatta
    30901 actggtaaaa gaatccatat caatatcgtg gaaatcaaaa aaccagatct agatgcaaaa
    30961 ttagtaggtg aaggaatcgc acgtcaatta gaaaaccgtg ttgctttccg tcgcgctcaa
    31021 aaacaagcca tccaacgctc aatgcgtgct ggcgctcaag ggatcaaaac tcaagtatct
    31081 ggtcgtctaa acggagcaga tatcgctcgt tcagaaggat actcagaagg aactgttcct
    31141 cttcatactt tacgtgcgga cattgattac gcatgggaag aagcagatac aacatacgga
    31201 aaactaggag ttaaagtgtg gatctatcgt ggagaaattc ttccaactaa aaaaaacact
    31261 gagaaaggag ggaaataatc atgttagtac ctaaacgtgt aaaacaccgt cgtgaattcc
    31321 gcggaaaaat gcgcggggaa gctaaaggcg gaaaagaagt agcattcggt gaatacggtt
    31381 tgcaagctgt tgattcacat tggatcacaa accgccaaat cgaagctgct cgtatcgcaa
    31441 tgactcgtta catgaaacgt ggtgggaaag tatggattaa aattttccct cacaaatctt
    31501 atactgccaa agcaattggg gtacgtatgg gttctggtaa aggggcacct gaaggatggg
    31561 ttgcaccagt aaaacgtggt aaaatcatgt ttgaaatcgc aggcgttcct gaagaagtag
    31621 ctcgtgaagc gttacgtcta gcttctcaca aattaccaat gaaaactaag atcgtaaaac
    31681 gtgaggaaat gggtggtgaa tcgaatgaag gttaaagaaa tcagagaatt aaccactgcc
    31741 gaaatgcttg atcaagaaaa acaattaaaa gaagaattgt ttaatcttag attccaatta
    31801 gcaacaggtc aattagaaaa cactgcacgt attaaagaag tacgtaaatc gattgcacgc
    31861 atcaaaactg tgttgcgtga acaagctaag taattatgga aggaggccat taagcgtatg
    31921 actgaaggaa gaaatcaacg caaagtttat caaggtcgcg tggtttcaga caaaatggat
    31981 aaaacaatta ccgttgctgt cgaaacaaag aaaaaccacc ctatctatgg caaacgcatg
    32041 aactattcta agaaatacaa agtgcatgat gaaaacaaca cagcaaaagt tggcgacatc
    32101 gtgagaatta tggaaactcg tccattatca gctacaaaac gtttccgttt gttggaagta
    32161 gtcgaagaag cagttattat ctaataacac gagattttcc tatatagatt gaatgcaaaa
    32221 tctgaaagga ggatacacaa cgtgatccaa gcagaaagtc gtttaaaagt cgctgataac
    32281 tcaggcgcac gtgaaatttt gacgatcaaa gtccttggtg gatctggtcg taaaactgct
    32341 aacatcggtg atgtgatcgt tgctacggtt aaacaagcaa cgccaggtgg ggttgtcaaa
    32401 aaaggtgacg tcgttaaagc cgttatcgtt cgaacaaaat caggagctcg tcgtacagac
    32461 ggttcatata ttaaatttga tgaaaatgct gcagtgatta ttcgtgatga taaaagccct
    32521 cgtggaacac gtatctttgg gccagttgca cgtgaactac gtgaaaacaa cttcatgaag
    32581 atcgtttcac tagcaccaga agttttataa tcttgagaca cttatcaaag gaggtgcgaa
    32641 acagttatgt ttgttaaaaa aggcgataaa gttaaagtta tcactggaaa agacaaaaac
    32701 aaagaaggcg ttgtactagc agcgttcccg aaaaaagata aagttattgt cgaaggtgta
    32761 aatgtcgtta aaaaacacca aaaaccttct caagctgctc ctcaaggtgg aattgttgag
    32821 atggaagcgc caattcatgt ttctaacgtg atggtggttg actctactgg tgtagctggc
    32881 cgagttggct acaaagaagt agacggcaaa aaagtccgtg tttctaaaaa aaccggtgaa
    32941 gttttagata aataaaagtt taaggaagga gggtcttact gaatgaaccg cctaaaagaa
    33001 aaatatctta aagaagtaac tccatcattg atggaaaaat ttaactatag ctctgttatg
    33061 caaacaccta aagttgaaaa aatcgtcatc aacatgggtg ttggtgatgc ggtatcaaac
    33121 gctaaaaact tagataaagc agttgaagaa ttaactttga tcactggtca aaaaccaatg
    33181 attacaaaag ctaaaaaatc aatcgctgga ttccgtttac gtgaaggaat gccaattggt
    33241 gcaaaagtta cattgcgcgg agaaagaatg tacgaattct tagacaaatt ggtttctgtt
    33301 tcactaccac gtgtacgtga cttccatggt gtaagtaaaa aagcctttga cggtcgtgga
    33361 aactatactt taggtattaa agaacaattg atcttcccag aagtagatta tgatttagta
    33421 gataaagtac gtggtatgga catcgttatt gtaacaacag caaactctga tgaagaatct
    33481 cgtgagttgt tgacacaatt aggcatgcca ttccaaaaat aattaaagga ggcgaactac
    33541 gtggctaaaa aatcaatgat tgctaaaaac aaacgtcctg caaaattttc aacacaagct
    33601 tatactcgtt gcgaacgttg cggacgtcca cattcagttt atcgtaagtt ccatctttgc
    33661 cgtatttgct tccgcgaact tgcctataaa ggtcaaattc ccggcgtgaa gaaagctagc
    33721 tggtaaaaag taaattcgca taagaaggag gtaaaagatc aatggtcatg acagatccaa
    33781 ttgcagattt tctaactcgt atccgtaacg caaacatggt taaacatgag tctttagaag
    33841 tacctgcatc aaaaattaaa cgtgacatcg ctgaaatctt gaaacgtgaa ggtttcgttc
    33901 gtgatgttga atacatcgaa gatgacaaac aaggcgtgat ccgtgttttc ctaaaatttg
    33961 gtaaaaacgg cgaacgtgtt atcacaaact taaaacgtat ctctaaacca ggtttgcgtg
    34021 cttatgtcaa agctgacgaa gtaccaaaag tattgaacgg tttaggtatc gcaatcattt
    34081 cgacttctga aggtgttgtc actgataaag aagctcgtga aaaaaatatc ggcggcgaag
    34141 ttatcgctta cgtatggtaa tcaagtagaa tctacaagga ggtgtcttta agtgagccgt
    34201 atcggaaata aagtcgttgt tatccctgaa ggagtaacag ttactcaaga cggaaacaac
    34261 gttacagtta aaggaccaaa aggtgaattg actcgtactt tttcagcaga tatcaaaatg
    34321 aatatcgaag gaaacgaagt aactttcact cgtccaaacg acagtaaaga aatgaaaaca
    34381 attcatggaa caactcgtgc gaacttcaac aacatggttg taggtgtcag cgaaggtttc
    34441 caaaaagcgt tagaactaat cggggttggg taccgtgcac aattacaagg atcaaaactt
    34501 gtattgaacg ttggttactc acatccagta gaaatcacac caccagctgg agtaactgtt
    34561 gaagtaccag ctaacacaca agtaattgtt aaaggtgcga acaaagaaga agttggcgaa
    34621 ttagctgcaa acattcgtgg aactcgtcct ccagaacctt ataaaggtaa aggtattcgt
    34681 tatgttggcg aatatgtacg ccgtaaagaa ggtaaaactg gtaaataatc gtttagaaaa
    34741 attgcataga agccagcagc aggaaaaagg catttggctg aagtttacct tcaaaaaaat
    34801 acatttttac ttgtgccgca gcttctattt ttcgaaacgt atttactgct tgaaatagta
    34861 gtacaataaa aataaagagg tgacaattgt gattacaaaa ccagataaga acaaaacacg
    34921 tcaaaagaga catcgtcgtg tacgtaacaa tatctctggt actgctgagc gcccacgctt
    34981 gaacgttttt cgttctaaca aaaacatcta cgctcaaatt attgatgacg tagcgggtgt
    35041 aacgctagca agtgcctcta ccttggataa agaaatttca ggtggaacaa aaacagaagc
    35101 agcacaagct gtcggtaaac tagttgctga acgtgcagct gaaaaaggca ttaaagtagt
    35161 agtctttgac cgtggtggat acctttacca tggccgtgtg caagctttag ctgaagctgc
    35221 tcgcgaaaat ggactagaat tttagaagaa ggaggaatac cattcatggt ttatatcgat
    35281 ccaaaacatt tggaattaga agaccgcgtt gttgcaatta accgtgtaac aaaagttgtt
    35341 aaaggtggac gtcgtctacg ttttgctgct ttagttgttg tcggtgacaa aaacggacac
    35401 gtagggtttg gtacaggtaa agctcaagaa gttcctgaag caatccgtaa agcaattgaa
    35461 gatgcgaaga aaaacttagt tgaagtacct atggttggtt caacaatccc acacgaagta
    35521 atcggtgctt ttagtggtgg tagaatccta atgaaacctg ctgtagaagg ttctggggtt
    35581 gctgctggtg gaccagttcg tgccgtatta gaattagctg gggtagcaga tatcacttct
    35641 aaatctttag gctcaaatac accaatcaac gttgttcgcg caacagttga aggtttgaaa
    35701 caattgaaac gtgcagaaga agttgcagaa cttcgcggca aatcagttga agaattaatc
    35761 ggataaggag gacaacaata atggctgaat taaaagttac tttaaaacgc agtattatcg
    35821 gacgtcctca aaaccaacgc gatacagtta aagcgttagg tcttacaaag attaacagtt
    35881 cagtggttaa gccttctaat gaagcaatta aaggcatgat caatactgtt tctcacctag
    35941 tggacgttga agaaatttaa tttagaaaaa tcataactag aacattaagg aggtgccaaa
    36001 cctatgaaac ttcatgaatt aaaacctgct gaaggttcac gccaagtacg taaccgtgtt
    36061 ggtcgtggta cttcatctgg taacggaaaa actgccggac gcggacaaaa aggacaaaaa
    36121 gctcgttcag gcggcggtgt gcgtttagga tttgaagggg gacaaacacc attgttccgt
    36181 cgtttaccaa aacgaggatt cacaaacgtg aaccgtaaag actatgcagt cattaatctt
    36241 gatgtattga accgctttga agacggagct gaagtaactc ctgttgcttt agttgaagct
    36301 ggaatcgtga aaaacgaaaa agctggaatc aaagtattag ctaatgggga actaaacaag
    36361 aaattaactg tgaaagcagc aaaattctct aaagcagctc aagaagcaat tgaagcggct
    36421 ggtggatcaa tcgaggtgat ctaatgttta gactattaaa agatgctttt aaagtaaaag
    36481 acattagatc gaaaatccta tttactgtat ttgtgttgtt tgtatttcgc ctaggtgcac
    36541 atattactgt accaggcgta aatgctgctt cattgcaaaa cctttcttct ttgccatttt
    36601 tcaacatgtt gaacttggta agtggtagtg cgatgcaaaa cttttccatt ttctcaatgg
    36661 gggtttcacc atacattact gcttctatcg tcattcagct tcttcaaatg gacatcgtgc
    36721 ctcgatttgt tgaatggtcg aaacaagggg aagttggtcg taaaaaactg aatcaagcga
    36781 ctcgatatct gacattagta ttggcattta tccaatctgt cggtatcact gctggattca
    36841 atcaatacgc tcaattaggt ttcgtgaata atcctaacgt agcaacttac gtaacgatcg
    36901 gtttgattct aacagccggt acgatgtttg ttacttggct aggagaacag atcacagaaa
    36961 aaggtatcgg aaatggcgta tcaatgatca tttttgcagg tatcatttct agattgcctg
    37021 aaagtatcag agaaatttat gaagattact ttataaatat tgatccttca gacatatgga
    37081 aatctgctat ttttgtggcc attttactag tagcggtctt gttgatcgtg acattcgtaa
    37141 catttttcca acaagctgaa cgcaagatcc cgatccaata tacgaaacgc gtagctggcg
    37201 cgcctacaag cagctatctg ccgttgaaag taaatgcagc tggagttatt ccagttatct
    37261 ttgctagttc gtttattgct acgccaaatg ctgttttaca agcattgagc ggtagattcg
    37321 gttctgaaaa ctggtacaaa gtgatgatgg agatctttaa ctataataca gcacctggtg
    37381 ctacgattta tacgattctg atcgttgcct tcactttctt ctacgccttt gttcaagtaa
    37441 accctgagaa attagcggaa aacttgcaaa agcaaggaag ttatatacca agcgtacgcc
    37501 ctggtaaggg gactgaagag tatgtctcaa gattactgat gcgtttaagt acagttggtg
    37561 cagtcttcct tggtttagtg gcattgcttc caatcattgc tcaaatggtt tgggatcttc
    37621 cacaatcaat cggattaggc ggtacaagct tactgatcgt tatcggtgta gctttagaaa
    37681 ctgcgaaaca gttagaaggc ttaatgctga aacgtaaata tgttggattc attgagtaag
    37741 aaagcgtgtt gaggtttccc ctcaacacac gttctcacac atttattagg agggaaaaca
    37801 atgaacctca ttttaatggg gctgccaggc gcaggtaaag gaacacaagc tgaaaaaatc
    37861 attgatgctt atggtattcc gcacatctca acaggagata tgttccgcgc agcgatgcaa
    37921 aacgaaacag ctcttggcct ggaagcaaag tcttatatgg ataaaggtgc attagttcct
    37981 gatgaagtaa caaatggaat cgtgaaagag cgactagctg aaccagatac agagaaaggc
    38041 ttcttattag atggctttcc acgtacgcta gatcaagcaa aagctctgga cgcaatgcta
    38101 aaagatttga ataaaaaaat tgatgctgtc atcgatatcc atgttggtga agaaatcttg
    38161 gtcgaacgtc tagccggccg atttatctgc agcaattgtg gagcaactta ccataaaatt
    38221 ttcaacccta caaaagttga agacacatgt gatcgttgcg gtgggcatga attctatcaa
    38281 agagaagatg ataaacctga gacggttaaa aatcgtctgg ctatcaacat caaaaatagt
    38341 gaaccaatct tggcttatta taaagaacaa ggtttgctaa acactattga tggtgctcgt
    38401 gaaatcgatg ctgtatttgc agatgtacaa aaaatcattg aaaaatagca cgattgcccg
    38461 tagccgactt gcattatgca gttactcttg tcaagttcaa taaattatga tagaatatgt
    38521 cagtccgact tctagagaat cctctagatg atccattttg agaattttga ggtgggaact
    38581 gcagtttccg ccattttatc gtgtgaaaat gcgaaacaag tacaaggagg tactgtgcgt
    38641 ggcaaaagaa gatatgattg aagtcgaagg tacagtcgtc gaaactttgc cgaatgcaat
    38701 gtttaaagtt gaactagaaa acggacacca agttcttgct actgtttcag gtaaaattcg
    38761 tatgcattac atccgtatct tacctggtga caaagtaacg gttgaattgt ctccatacga
    38821 tcttaaccgt ggccgtatta cttatcgctt taaataattg tactccgtaa aactacagga
    38881 ggtattatca tgaaagtaag accatcagta aaaccaatgt gcgaacattg taaagtaatt
    38941 cgtcgtaaag gacgcgttat ggtgatttgc ccagcaaatc caaaacataa acaacgtcaa
    39001 ggataattgg aggtgctata aatatggctc gtattgcagg agtagatatt cctcgtgata
    39061 aacgtgtagt agtttcactt acttatatct atggtattgg taacactact gctaaaaaga
    39121 tcttagctga tgctggtgta tctgaagatg ttcgcgttcg tgaattaacg aatgaacaaa
    39181 cagatgctat ccgtgcagaa gtagataaat taaaagttga aggggatctt cgtcgtgaag
    39241 tgaacttgaa catcaaacgt ttgatggaaa tcggttctta ccgtggaatc cgtcatcgcc
    39301 gtggattgcc tgttcgtgga caaaacacga aaaacaatgc acgtactcgt aaaggcccag
    39361 ctcgcgctat cgctggcaag aaaaaataat tcttaagtga aggaggttaa aacttcatgg
    39421 cagcaaaaaa agtgaatcgt aaacgccgtg tgaagaagaa tatagaagca ggtattgcac
    39481 atatccattc tacattcaat aacactatca tcatgatcac tgatactcac ggaaatgcat
    39541 tagcgtggtc atcagctggt tcattaggtt tcaaaggaag caaaaaatca actccttttg
    39601 ccgctcaaat ggcagcagaa actgctacaa aagcagcaat ggaacacgga ttaaagactg
    39661 ttgaagtaac agttaaagga cctggttctg gacgtgaagc agcaattcgt tcattacaag
    39721 ctacaggttt agaagtgact gcaattcgtg acgtgactcc agttcctcat aatggtgccc
    39781 gccctccaaa acgccgtcgt gtttaatgag tgccgctcat tttgagtttt atcagtaacg
    39841 tcattgaaca agacactttt cgttttgaaa ggggtaaaga taagaatgat tgagttcgaa
    39901 aaaccaagaa tcgcaaaaat tgatgaagaa aaagattatg gcaagttcat cgttgaacct
    39961 cttgaaagag ggtatgggac tactttaggg aattccctac gtcgtatttt attatcttct
    40021 ttgccaggtg ctgccatcac taatattcaa atcgatggtg tactgcatga attctctacc
    40081 atcaaaggtg ttagagaaga cgtcactcaa atcatcttga acattaaagg cttagcgctg
    40141 aaaatgtatg gccaagaaga aaaaaccctt gaaatcgata ttaccggacc tgctacagta
    40201 actgctggcg atattatcgt tgatagtgat gtagaaattc ttaacaaaga tatgtacatc
    40261 tgtagtgtct ctgaaggagc tacgttccat gctcgcttaa cagtgaaacc tggacgtggt
    40321 tatgttcaag cagatgaaaa taaaaaagaa gatatgccaa ttggcgttct tccagtcgat
    40381 tcaatctaca cacctgttcg tcgtgtgaac tatcaagttg agaacacacg tgttggacac
    40441 cgtgaagatt ttgataaatt gacgatggag atttggacag atggttcaat tgaaccgttg
    40501 gaagcaatga gtttgtctgc gaaaatcatg actgaacatt tggatatttt tgtgaactta
    40561 actgatgaag caaaaaatgc tgaaatcatg gtcgaaaaag aagaaacaca aaaagaaaaa
    40621 atgttggaaa tgacgatcga agagttagac ttgtctgttc gctcttacaa ttgtttgaaa
    40681 cgtgcaggaa tcaatactgt acaagaactt acaaataaat ctgagcctga aatgatcaaa
    40741 gtccgtaact taggacgtaa atcattagaa gaagttaaag caaaattgca cgacttaggt
    40801 ttaggtttac gtaaagaaga ttaaaacctt ttacgaagga gggaaaccaa cgtgagttat
    40861 cgtaaactag gacgcacatc tagccaacgt aaagcgatgt tgcgtgattt aacaactgat
    40921 ttattaatca acgaacgtat tgtgactact gaagctcgtg cgaaagaagt tcgctcaact
    40981 gcagaaaaaa tgatcacttt aggtaaacgc ggagatttgc atgcacgtcg tcaagctgcg
    41041 gctttcgtac gtaatgaagt tgccagcgtg cgtgaagaaa atgaggacat cgtcattgaa
    41101 tcagctttac aaaaactgtt caacgatatt gcacctcgtt acgcagaacg tcaaggcgga
    41161 tacacacgca ttttgaagac agaaccaaga cgcggtgatg gtgcaccaat ggttgtctta
    41221 gaacttgtct aaaatcacac taataacatc actttataga tgattctttt agagtgttat
    41281 gatgttggaa gatatttttc cgagtctagc tcagcgctgc tcctcaagcg aatatcgctt
    41341 ggggagtaag cactcatcat aaagtgtttt tttatacctt caattacctg tcatgacaga
    41401 tatgtttgaa ggcataaaaa aacattttaa gcgagaaata attgaaaatt ttgtcttttt
    41461 ttgatagtgt tagtaggaac tttaggatga agaaggagta atcatggagc caattattga
    41521 attagaaaag ataaattata aataccagcc agatgatctt cgccccgcat tgaaagatgt
    41581 ctcctttaca attgataaag gtgagtggat cgctattatt ggacacaatg gttcaggaaa
    41641 atcgacactt gccaaaacga tcaatggatt gcttctgcca gaatcaggga tcgtgaaagt
    41701 gggaaatcaa atattggatg aagaaaatat ttggacgatc cgacagatgg ttggaatgat
    41761 ttttcaaaat ccagacaatc agtttgttgg ttcgacagta gaagatgacg ttgcttttgg
    41821 tcttgaaaat caagggattc ccagagaaga aatgctagtg cgtgtgaaag atgcacttga
    41881 gaaagttaga atggctgagt ttgcttcacg cgagcctgct cgtttatcag gagggcaaaa
    41941 acaacgtgtt gccattgccg gcgtggttgc cttacgacct gatatcatta ttttagatga
    42001 agcaaccagt atgctagatc cagaaggtcg tgaagaagtg atctctacga tcaagaagat
    42061 caaagaagaa agtcaattga ccgttatatc tatcactcat gatattgatg aagcagctaa
    42121 tgccaatcgg atactagtga tgagacaagg cgaacttgtc cgtgaaggta caccaaaaga
    42181 aattttttct gctggtcctg aactgatcga cttaggtcta gatctaccat ttcctgaaaa
    42241 actgaagagc gcactgaaag aacgtggggt tgatgtgccc agtgaatata tgacagaaga
    42301 aaggatggtg gattggttat ggacatccgt tttgaacaag tagacttcac ttatcagccg
    42361 aatacaccat ttgaacaaag agccttattt gacatcaata tgacgatcaa agaaaacagt
    42421 tatactgcat tagtagggca taccggaagc gggaaatcaa ccttacttca gcatttgaat
    42481 gcgcttgtta agccaacaag cggtacagtc catattggag aacgagatat tcagccggat
    42541 acggataata agaatttaaa gcccatccga aaaaaagttg gcatcgtctt tcagtttcca
    42601 gaagcacagc tattcgaaga aacggtagca aaagacattg ctttcggtcc taaaaacttt
    42661 ggtgtcagcg aagaagaagc actagtccta gcaaaagaaa cattagaaca agttgggctg
    42721 gatgaaagct atttggaacg ttcgccattt gaactttcag gagggcaaat gcgtagagta
    42781 gcaatcgctg gtgtgcttgc catgaggccg gaagtacttg tattggatga accaacggca
    42841 ggacttgatc cccagggcag aaaagaaatg atggagatgt tttggcgtct tcataaggaa
    42901 cagcagatca ctattgtatt agttacgcat ttaatggatg atgtagccaa ttttgcagat
    42961 tatgtctatg tgttggaaaa gggacgaatc gttaatagcg gggagccgca agaggtcttt
    43021 caaaacattg aatggctaaa agaaaaacaa ctaggcgtac ctactgccac cgagtttgca
    43081 gaagaattaa tggctaaagg gatgaacttt gctaccttgc ctttgacagc tgaagaatta
    43141 gcagacgcta tcgtccatta tgctggagga aatgcccatg atgaataaat tgatatttgg
    43201 tcgttatatg cctggagatt ccttgatcca tcgattggat ccccgagcta aattattagc
    43261 cagtttttat tttatcggaa tcattttttt agcaaataat tgggaaacgt accttttgtt
    43321 ggcatttttc acattacttt ctgtcttctt gtcaaaagta gatttgaaat tttttatccg
    43381 aggcattcgc ccattgatct ggctaatcct cttcactgta gctttgcagg tcttgtttac
    43441 aagtggcgga gaaacttatt ggagttgggg tgttttccat atcactgaat ttggtgtgat
    43501 gaacggcctc tttattttct gtcgattcgt gttgatcatt tttatgtcga ctctactcac
    43561 gcttactaca ccgcctttgg aattatcgga tgcgattgag tacttgcttc gtccattgaa
    43621 agccgtacgg ttcccagtcc acgaaatttc attgatgctg tcgattgctc ttcgttttgt
    43681 gcctacattg atggacgaaa cagaaaagat catgaacgct cagcgcgctc gcggtgttga
    43741 ttttggtgaa ggaaacctag ttcagaaaat gaaagcagtc attcctttgc tgattccttt
    43801 atttgtcagc agcttcaacc gtgcggaaga tcttgcgact gctatggaag caagaggcta
    43861 caaaggtggg gaaggaagaa cgaaatatcg tgtattgcat tggcataata gagacacgat
    43921 ggttatcatt gcttttgtat tattaactgt gctactgatc tttttaagag gataaacgtt
    43981 cagtgggccg ctatctattc ttttcttgct gatctgtttg caagaaagga tgcaagcagt
    44041 gtatgacaag aaatattagg gtctgtggca gactaaggtc acaggcctct ttcttttatc
    44101 tgaaaagaaa gaacgaaaaa attttataga aaaatcatta ttccagataa ccttttagga
    44161 acaaaggaga gaaaaacatg gtacgttaca aagcaatcat tgcgtacgac ggaacaaatt
    44221 ttaatggatt ccaaaaacag ccaaatgggc gtactgttca agaagaagta gaaaaaacat
    44281 tacaaaaaat ggcaaatgga aaagaaatca cggtctttgg ttcgggaagg acagatgccg
    44341 gagtacatgc gatcgggcaa gtaatccatt ttgattatcc agaggagcgt ccgttagaac
    44401 gaatgcgttt cgcgctagat acccagtcac cagaggatat tgctgtaaga caagtagaaa
    44461 ttgtttcaga agacttccac gcaagatatt tggtcaaaga aaagacgtat caatttcgag
    44521 tggatatagg gaaaccacgc agtccttttc gacgccatta tgcaagttat tttccttacc
    44581 cacttgattt ggaaaaaatt cagcgggcgc ttcctgattt attagggact cacgatttca
    44641 cctctttttg tgcatcagga agctcgatag aggataaaat acgtacgatc tacgaggcta
    44701 agatggaggt aaatgaaaca aaagacgaat tgttgtttac atttagggga aatggcttct
    44761 tgtataagat gatacggatc ttagtaggaa cgcttttgaa aattggcaat ggccgattgc
    44821 ctgaagatag tatcccagat attattgcga aaaaagatcg taatgcagca ggacctacag
    44881 cacaccctga aggactttat ttgtatgaag tgaaatatga ataagaggtt atgatagaag
    44941 tggtcagctt caagaaataa gaaggtattt acgaaaatta cttctcaaaa ttttgcaaaa
    45001 tttcggctta ttttcgaaga agctacttct ggaacaccgt ttaagaaaga ggtcgtgagc
    45061 ctgtcattta actctgagtc acaaagagca atttcaaaaa acagcttctc atctttgatg
    45121 aatatcgtga cttgtggacg aggcgttgat aagtgaatca tctttttgtt acctattttt
    45181 tctgtaacca aaaaacgatg gaacgttgtt ttgattgccc ttggtgcgga cagtctgaac
    45241 aggttccatc gtttctttat tttctacgtc ttcttctgtg tgtgtttcgt ggttgttctt
    45301 ctattgctgg atcaatcgtg ccataaaagg ggcgattgcg gtcaaggtac cagacataaa
    45361 gaataaacaa tgcaatacat aggacaaaaa ggattgcacc tatcgtaaat ccttcaggca
    45421 agtaactgaa agtgacagta tgtttacctg ctggaacttt tatcatcaag aatgcgtctt
    45481 tgaaattgct gaccttgact ggtttcccat caattttagc tttccatcct ttgtcataag
    45541 gaatagttgt caccaacatg gaatcttcag cggtatttac agtcccagaa gcttttctgc
    45601 cagtggcttc catttcgaca cctttttctt tgatctgatt cattgcggct tgataggcta
    45661 caacatctag accaacgact ttaggctcca tgaaacttat ttctttcgtc ccataaaaac
    45721 ttgcagtaaa tgtcactgtt gtctcttgag gataatagcc aagatcatag tattgaccag
    45781 aaatgttgat ttgcgttttt cgcgatgtac cattgacagt gatcgtcgca ctagaacttt
    45841 ctaattgcgc aaaattggtt gggaacaaac ttaagtaagc ttgtgtattt gcaggcacat
    45901 ggacttccca agtgagctct tttggctggt tatctgctgt ttctgagtaa gtgacgccag
    45961 tcgttgtctt tgtgatcgtc gtattcaact gattcttcaa agtaggtgta tagaatgtaa
    46021 aatactgttc atttagacca gacaatgtat tgatcaagtt tgtctgactg gtcaaattat
    46081 ctgtcactgg ctgctcaaca tcattaacgg acaacggtgc agtaaatgct agtggcaatg
    46141 cattgacatt ctgatacagt gtatagtcgc cgctttgacc agcttggcgg aagccatatt
    46201 tactgaacgt gctttcgttt ttagaaatgt tatatttgat tcctgtaaac ccatccatca
    46261 gcaaagtatt attgttataa cggatattca aatttgtccc tcttgaacgg aaacctaatt
    46321 tatccaggta agaggaagaa ttacgatttc ttatagaaga aaacaaactg ataccactgt
    46381 aaccatagtt gatcgaatcg tttgcagata ctggatccag attttccaag cggtaaaacg
    46441 agtcggaatc tttttttgtt tcatttacaa gagctttcaa gtcaggataa ggatcagtat
    46501 ataaactgcg ggaagcataa ttccaatcat caagaatgcc atggatcata ctgttcgtat
    46561 tgaccagtgc ttcagcagac atgatcaata ataacaatat actcagatga ttgaatgaaa
    46621 gttttttatg ctgataaaat cccacagcaa aagcataaat ccctaagaaa accactgtca
    46681 atacaaagga agtcatcgtc acataggtgt agcttccttc aggtttgatg ccccatgcca
    46741 ccgcaaagag agcagctacg accaaaatga ttcctaacaa cagcccacca tcgtcttgtt
    46801 ctatcttttc ccatcctttt cctgcaagca ggagcactaa gaaagagaac agataggcat
    46861 aacggaataa aaacatattc ggtgcatgca taccatgcca gaacaaattc aatggaacaa
    46921 gataaaaact tgcgataagt atggcgaaaa gactgccgta caaaaattta tttttcaacg
    46981 gtactttccg actggcaaaa taaaacagac agaggactaa tggtagcaga cctgcgtaaa
    47041 cgaacggaat cgatccgtat tttgtcgtgt cataaacacc aatcatattt ttcatgatta
    47101 gatccagata agaagtcgct tctgttttca acgtagtgat ctgactcagt gcctcgccat
    47161 ttgaccgaag atccagcact gctggcaata caatgatcat tgaagcgccg cctgccagta
    47221 aagaagtgat accataagga acaatcgttt ttttatattc tgaccagttt gtcaatagac
    47281 gggcgataaa atataagaaa gaaaatacgc caatcataaa acccatataa aaactggata
    47341 aaaacaagag taaataactg ataaataaaa gtttcggccg tttaagatcc ataacacgat
    47401 gaatccccaa aatcactaat ggaagataaa caaatgcatc cagccacatg atgatctccg
    47461 aatgtgcagt gataaatgag atcaatgcgt aactgacgct aagtgcaaca tgcccccagc
    47521 gaggaatctt gaacgtacgt gcaagaaacc aaaaagccaa tccggcacag ccgaccttaa
    47581 gcaaagtcag aacatacagt gcatctggca tcatctggtt tggaaaaaag aacaccagtg
    47641 gcgtgaagat cccgcctaga tagtaagaaa ttaaagataa ataattcaat cctagagaag
    47701 cattccatgt ataaaaaata ctttgctttc caagaagaac atttcggaag ctggcatgaa
    47761 aattagaaaa ctgtgaaaag gcatcacttg ccaaaatact tctgctgctt ccaggataga
    47821 ttccgatact tagataaaca agtgctaaaa tcaaaaacgg aatcaggaaa ctagctgctg
    47881 cgtagcgtcc gttttctcta aagaatgact ttaaattttt tttcatattt attttccacc
    47941 aaatttctat gaagttgtat tcagtttatc atatccttgt ttttctctgc ttaaaaagag
    48001 tctagttttc acaaaaaaat aaaatttaga ataaataact tcataatttg ttcgtataac
    48061 aaaaataaac gcaaaaaaag ggtaggaatc aaaagtccca cccttaaaaa tttttattaa
    48121 ataaaaaacc gttcactatc tgaacggtcc aagtatcatt caattaaacg cgttcaactt
    48181 ttcctgattt caaagcacga gttgacaccc aaactttttt aggttttccg tcgaccataa
    48241 cgcgaacttt ttgtaagtta ggtttaacag tacgttttgt tgagttcata gcgtgtgaac
    48301 ggttgtttcc actttttgtc ttgcgacctg taaagtaaca aacttttgcc atggtaattt
    48361 cctcctttgc cttgatttgg agcaatagaa taattcagct cataccagac taacatatca
    48421 tatagaatgg gaatttgcaa gtacattttc atagatacaa ttgacaattg agaagaataa
    48481 ttgtaaatta aacaaggtta tacctgaaat tttgcaaggt ttttgagtac tttgggtaac
    48541 tgccttttat ttcaagttgc gctatgataa aatatgatag aagaaaaatg ggagtttttt
    48601 acccgttaag gaggctcatt catggctgta aaaattcaaa cgtcagctgg aaccattgaa
    48661 attaccaatg atgtaattgc aacagttgtt ggtggcgccg cgacagatgt gttcggtgtt
    48721 gtaggaatgg ccagcaaaaa tcaaattaaa gataatatta ctgagatttt gcgtaaagaa
    48781 aattactcaa gaggagtagt tgtccgccaa gaagataatg gtattgctgt agatgtttat
    48841 acgattatca gctatggtac gaaaatctct gaagtttccc gcaacgtcca agaaaaagtg
    48901 aaatacaatc tagaggcatt attaggcgta actgccaatt cagttaatgt ctttgtacaa
    48961 ggtgttcgtg tactgccaga ctaggcagga gactggctgg taactcagca gtcgatctga
    49021 aggaggatta tctaggtgag tgtaacagaa ataagtgcaa gccagttcca ggaaatggtc
    49081 caggcaggtg cgaatcgtct gcaagtaaat gcagaatacg tcaattcatt aaatgtattc
    49141 cctgtgccag atggcgatac gggaacaaat atgaatctat ctatgactag tggagctaag
    49201 gcagtagtag attcaacctc agaaaaagta ggagaattag cagcaatgct ttcaaaagga
    49261 ttgctgatgg gtgcgcgcgg aaattccggc gttattttgt ctcaattgtt tcgtggattt
    49321 tctaagcaaa ttccagatgt tgtctcatta aatgcttctg atcttgctgc agccttcact
    49381 catggagttg aaactgcgta taaagcagta atgaaaccag tagaaggaac gattttgaca
    49441 gtagctagag aagcagcgaa agctggagaa aagaaagcaa aaagtactga tgatgtcgtt
    49501 gaagtaatga cagctgttgt aaaaggagca aagcgtgctt tagctaaaac tccagacttg
    49561 cttccagtcc taaaagaagt cggtgtcgta gacagtggag ggcaaggact gctgtttgtt
    49621 tatgagggct ttttaagtgc attgaatgga gaattccaag cggatgaaac atatgaacct
    49681 tctccagcag aaatggatga tatggtcaat gcagaacatc atcgcagtgt ccaaggtcaa
    49741 atggcgacag aagatatcca ttttggttac tgtacagaaa tcatggtaaa aatcggagaa
    49801 ggacctacag tcgatagtac attcgattac gaagaattcc gtaactattt agatggtata
    49861 ggagattctt tacttgtggt caatgatgac gagatcatca aagtccacgt gcatacagaa
    49921 aacccaggag aagtcttgaa ttatggacaa aaattcggtt cattagtcaa aatcaaagtc
    49981 gacaacatgc gcttgcagca tgagacgatt ttggaacacg atgaagaagt gaacgagttt
    50041 gcgaaaaaag aaataccacg tatcccgtat ggtatcgtag cgatcgctgc tggtaaaggc
    50101 gtccaagagc tgtttgaaag tttaggcgct aattatgtta tcagtggtgg acaaacaatg
    50161 aatccaagta cggaagacat tgttaaagcg attcaagaag taaacgcaga taaagtgatt
    50221 gtcttgccaa acaataagaa catcttcatg gcagctgatc aagcagcaga agtagcagat
    50281 atcccagtag cagttgttcc ttcaagaacg atttcccaag gattgacagc tatgctttca
    50341 ttcaatgatc aagcttcttt agaagaaaac aaagaaacga tgacagaaat gttgtctagc
    50401 gttgtgagcg gtcaagtcac tcacgcgatc agagatacct caattgacgg tgtagagatc
    50461 aaagaaggag actttttggg catgatcgat ggaaaaatcc tgatctctaa cccagatatc
    50521 acagcaacag cgttaagtac actagaacaa atgatcaatg aggacacaga gatcgtaacg
    50581 atcttggcag gtgatgaagg aacagttgaa caagcaaatg aatttgctga gaaactaaca
    50641 gaaaaacatc ctgatttgga aatcgagatc caccaaggag atcagcctgt ctatccttat
    50701 ctattttctg ctgaataatt tctgtgtcag aatatctttc taacacagta ttcattcata
    50761 aaaggccttc ggttagtgat caggtatgat cgctgaccaa gtcctttttt tgttaaagga
    50821 gggaggaaaa atttatgcgt tctttagatg atccaatcac tatgctggca ggagtcggag
    50881 aaaaaagagc agctagtctt gccagcttgg gcatccaaac gattgaagac ttactgactt
    50941 attatccttt tcgctacgaa gatattcagg aaagaaatct aaacgagatc caggatcaag
    51001 aaaaggttac tttgaaagga atcgtagtct cgcctcctgt gatgaatcga tttggttata
    51061 aaaaaagccg attgcaattt cgtatgatgc aagaacatga tgtgttcaac gtttcttttt
    51121 ttaatcaacc ttatctaaaa gaaaaagtca ttttatcgga ggaaatcgcc gtatacggta
    51181 aatgggatgc aaagcgtaaa gccttgaacg ggatgaagat tttaggttct caaacaatgg
    51241 atgacttttc gccgatatat cacgtcaaca aatcaattcg tcaaacgaca ttagttgatc
    51301 taatcaaaaa agggtttcaa gaatacggca cattgattga agaaaatctg ccaaacaacc
    51361 ttgtggagaa atatcgactg cttgaccgag cttctgctgt aagaagtatg cattttccaa
    51421 aagaccacga agaaaaccat caagccaaac gacgtgtcgt ttttgaagag ttctttttat
    51481 ttcaaatgcg tcttcaagga ttgaaaaaag cagaaaaagc agaaacgaat ggtttggaga
    51541 tcctttatga tgtccaaaaa ttgaaacaat ttactcaaaa gctacctttt gaactgacaa
    51601 atgcacaaaa acgtgtaacg aatgaaatct gtcgggatct tcgcagtccg cagcatatgc
    51661 agcgactttt gcagggagat gtaggtagcg gaaaaacagt ggttgccgcg attgcgttat
    51721 acgctaccgt tacagcaggt ttccaaggtg cgttgatggt gcctacagaa atcttggcac
    51781 aacaacatat ggaaagcttg caacagctgt ttgatcccaa tgaagtaacc atcgcattga
    51841 ttacggggtc aacgaaagca aaagaacgac gtgagctcct tgaaaaacta gagcaagggg
    51901 aaattaacgt cgtgatcggc acacatgcat tgatccaaga tggagtcgaa tttcagaatt
    51961 taggtctagt tattacggat gagcagcacc gttttggcgt gaaccagcgg aaagtacttc
    52021 gtgaaaaagg ctggcgacca gatgtcttat ttatgacagc gactccgatt cctagaacat
    52081 tagctattac agcatatggt gagatggatg tatctatcat tgatgaactt ccagctggac
    52141 gtatcccgat cgaaacacgc tgggtaagaa cacctcaatt ggacagtgta ctggattgga
    52201 cgaaaaaaga actggcaaga ggacatcaaa tgtatgtcat ctgtccgctg attgaagaat
    52261 cagaagcatt agatgtaaaa aatgcagtgg aaatctatga aaaacttcgt gaactatttg
    52321 caccaaaata tgaagttggc ttgttacatg gtaaaatgaa gaatcaggaa aaagaagtaa
    52381 tcatggaagc cttcaaagac aaccagatgc agatattagt ttcgactact gtaattgaag
    52441 taggggtaaa cgtgccgaac gcgaccgtca tgctgatcat ggatgcggat cgatttggat
    52501 tggcacagct gcaccaactt cgtggacgtg ttggacgcgg aagtgatgct tcctactgta
    52561 tcttagttgc taatcctaaa aatgaactag gtgtggaacg gatgaaaatc atgacagaga
    52621 ccaccaatgg ttttgtacta agcgaaaaag atctggaatt acgtggtcct ggtgaagtat
    52681 ttggcttccg tcaatctggt ctgcctcaat ttgcgattgc tgacttggtg acggatggaa
    52741 atgttttgga ggtagccaga gaagaggctg cagccctttg gcaaataaag gattggcaga
    52801 tgcttcctga gtatcagcct ttagcgcaaa gcctccaaat agaaaatcag gataatcgtt
    52861 atttcgatta gcagactgtg ctatacttta tactgatgac ggaaaaagga gtggaaacaa
    52921 tgaaaattgc cgtagatgca atgggcggag atcacgcacc tcaagcaatt gtagaaggcg
    52981 tggctttagc gcagcaagat tttccagaag tagaattctt actttacgga aaagaagcag
    53041 aaatcaagaa atacttaaca aatgaaaaaa acatcacgat tatccataca gacgaaaaaa
    53101 tcaatagtga tgatgagcca gtaaaagcaa ttcgacgaaa aaagactgca tcaatggtcc
    53161 ttgctgcaca agcagtgaaa aacggtgaag cagatgctgt attttcagca gggaatacag
    53221 gcgcgctact agcagcaggg ttgttcatcg taggacgtat caaaaatatc gaacgccctg
    53281 gcttgatgtc taccttgcca gtagtaggga aagaaggtgc cggtttcgat atgctggatc
    53341 ttggcgctaa cgcagaaaac aaaccagaac atttgttgca atacggtatt ttaggttctt
    53401 tttatgcacg taaagtccga ggtatcggac gtcctcgtgt cgcactctta aacaacggaa
    53461 cagaagcaac aaaaggtagt gaagtgacga aaaaagccta tgaactcctt cagaatgaaa
    53521 caagcttgaa ttttattgga aacgtagaag ctcgtgagct actcaatggt gtggctgatg
    53581 tagtggtcac agatggattt acaggcaacg ctgttttgaa atctattgaa ggaacagcaa
    53641 tgaatatgat gaatctatta aaatctgcta ttctaaatga agggatcaaa ggaaaaatgg
    53701 gcgctatgct tttaaaagac ggcttacgtt ctttgaaagc agaaatggat tattccaaac
    53761 atggcggtgc cgttttgttt ggcttgaaag caccagtcat taaaactcat ggagcaacag
    53821 gccccgatgc tgttcgctat acgatccgcc aaatccacac aatgcttgag acagatgtag
    53881 ttggacaatt agtcgaacaa tttgaaaaaa gcgaagaata gtatttggat atctgaagcg
    53941 atccgttcca ttgaaaaaaa ggtggacatt cctgaaaaat gccagtaaaa ttagccatgg
    54001 tgtaggtaat tgacaagtgt atgattcttg gaggtgaaaa agttgactcg cgaagaagta
    54061 tttaacaaag tagcgaaaat tatttcaaat cattttgaga tagataccga taaagtgacg
    54121 gatgaattga acatcaaaga tgatttaaaa gctgattcaa ttagtattat ggaattcgtt
    54181 ttagagcttg aagatgaatt cggtacggaa atttcagatg aagacgctga acaaattgaa
    54241 acagtcggtg gcgcagtaga ttatatcagc agccatctta aataatttca aataaaagca
    54301 aagaaggata aaaagctgca tgcagctttt tatccttctt tttttaatct ttttgacacg
    54361 taaaagtcat ttttctgata attctggcga taaacagaaa ttatgttgca aagtaaagcg
    54421 gttttctata taattattaa taattcatta cacaaagata agaataacct ttgttataaa
    54481 acgatttatt ctgtgtaatg tcctttttta ttaagaaaaa cttccattta ggaagcagag
    54541 aggggtattc gattgtccga taaccaatta ttagaagttc agaacctaca tacaggattc
    54601 cgactcaaag atgactatta cgatgcagta gatgacgttt catttacttt agacaaaaac
    54661 gagattttag caatcgttgg ggaatctggg tgcgggaaaa gtaccttggc tactacgatt
    54721 atgggattac ttgatcctaa taacacaaaa attacaggtg agattttata taacgatctg
    54781 aatttaatcg acctgaacga aacgctctat aataaaatcc gcggaaatga tatcggaatg
    54841 attttccaag atccgttgtc tgcattaaat cctctcatga gaatcgaaga ccagatcaaa
    54901 gaaggacttt cctatcacac aaagatgaat gcagaacagc gtcaagctcg tgcactagaa
    54961 ttgctagaac aagtagggat tcctaatcct gcgagagttg gacgccaata tccccatgaa
    55021 ctatcaggcg gtatgagaca acgtgtcatc atcgcaatcg caatcgcctg caaacctcca
    55081 atcgtcattg cagatgaacc gacgacagcc ctagatgtaa ccatccaagc acagattctt
    55141 gatttactga aagaccttca ggaagaaaca aaaacaggga tcatcttgat cactcatgac
    55201 ttaggagtcg tagcagaaat ggctgatcga gtggcagtga tgtatggcgg acagttcgtt
    55261 gaagtagcag gtgtaaaaga actgtttgat catcctaagc atccttatac acgctcttta
    55321 ctgaactcta taccacaaga aggaaatcat gatgcagaac tccatgtgat cgaaggtgtc
    55381 gttccttctc tgaaaaacat gcctcgtaaa ggttgtcggt ttgcaccacg tattccatgg
    55441 ataccagcaa gcgatcatga agaagagcct atccttcatg aagtcgcacc gaaccacttg
    55501 gtacgttgct cctgctataa gcatttccac tttagagacg aaaaaggaga ggtgtagatg
    55561 acagaattaa tcacaatcaa agacctgaaa gttcattacc ctattcgcag tggattcttc
    55621 aatcgagtaa cggatcatgt atatgctgta gatggtgtgg attttattat cgaaaaagga
    55681 aagacatatg gtttagtggg agagtctggc tctggaaaat ctactacggg aaaagccgtt
    55741 gtgggattag aaaaggtaac agccggacaa atcatgtatg aaggaaaaga tgtaacgaag
    55801 cgaagcaacc gcaaaaaaat gggttataac aaggatgtgc agatgatctt ccaagattca
    55861 atgtccagtt tgaatcctaa aaagcgagtg ttggatatca ttgctgaacc catccgaaat
    55921 tttgaacggt taagcgatca agaagaaaag aaaaaggtaa aaggactgct agatatcgtt
    55981 gggatgccgg aagatgcgct atataaatat cctcatgaat tttcaggcgg acaacgtcaa
    56041 cgtctaggag tagcccgtgc tgttgctacc aatccaaaac tgatcgtagc cgatgaacct
    56101 gtatctgctc tggatctatc tgttcaagcg caagtattga atttcatgaa acgaatccag
    56161 caagaatacg gcctcagcta tttattcatt tcacatgatt taggtgtagt aaaacacatg
    56221 tgcgacaata tcgcaattat gtacaaaggt cgatttgtag aaatcggtac aagagaagac
    56281 atttataatg acccgcgaca tatctatacg aaacgtctgc tttcagctat tccgcaaatc
    56341 gatgtagaaa atagggaaag ccacaaagaa aatcgacgaa gagtagaaca agagtatatc
    56401 caaaaccaaa aagaatacta tgatgcgact ggtcgagtct acgatttgcg tacgatcaca
    56461 actacacaca aagtggcgtt gaaagatgga ggtgcaagct aatgtggaaa acgattctgc
    56521 gacgtgtttt attgatgatc ccgcaagtta ttattttaag tgttctgatc ttcttattag
    56581 cccaagcgat gccgggggat ccatttaccg gtttgatcaa tccaaatcag gatccggcag
    56641 tgatcgaaca gatgcgacaa gcagctggct tgaatgaccc atggtatgtc cagtacttcc
    56701 gttggatcgg aaatgctttg caaggcgatt ttggtcaaag tttcttatac aaacgctctg
    56761 ttgctgactt gatcggcgaa cggatcatca acactttata tctatcgatc ttgacagttg
    56821 tccttactta tttgattgct gtgcctctgg ggatgcttgc tggccggtat caaaattcaa
    56881 tcttggataa gattgtcgta atctacaact tctttagttt tgctgttcct ttatttatct
    56941 tcggcttgat catgctattt gtttttggtt atcgtttagg ctggttccca acaagtggat
    57001 cacaaacact aggtttgcaa ggtggattta tcactcaatg gatagaccgt ttgaaatata
    57061 ttctgttacc atcaattacc caagcatttt tggcgactgc cgtcacaatc caatatctgc
    57121 gaagtgaagt aatcgattcg aaatcgctcg attttgtcag aacagcccgt tccaaagggg
    57181 taccgacgaa taaagtattc agtcgacata ttttccgaaa tgcagcgttg ccgatggctt
    57241 ctcaaatggg gtatgaaatc acttcgttga ttgctggttc tgtagttatc gaaaagattt
    57301 ttggttatcc gggagtcgga aaactattta ttgattccat cggccaacgg gactataccg
    57361 tgatcacatc cttagtattg attttaggaa tcgcgacgct agtggggaca ttactttcag
    57421 atatcatcat gagtatcgtt gacccgcgta ttcggattca ataaaaccaa tcagaaagga
    57481 gaatggatcg atgagtgaaa aaaacaatac agcagttcaa gaaagtattc ctcctatggg
    57541 gatacgaatg attgctcgag aatttaaaaa agataaaatt gctatttttt cattaggttt
    57601 gctagtggtt ttattattag tgatctttat cggtgcgatg ctgaccgatc aagacaaagt
    57661 gatgtatgtt agtatcttcg acaaatacgc tgcaccagga acagtttcga cacaaggaca
    57721 aaagttcatt ctgggagcag atgaaggcgg acgtgatgta ttaggacaat tgatcatagg
    57781 tgctagaaac tctgttacga tcggatttgc gatcacgatc cttacttcga tcatcggggt
    57841 tggattaggg atcatctctg gctactacgg agggatcatt gacaacatct tgatgcggat
    57901 cgttgatttt atcatgattc taccgatcat gctgatcatt atcgtcttcg tatcgattat
    57961 ttccagatac aatgtctggt cctttatatt catcatgagt gcattttatt gggtagctaa
    58021 agcccgatta ttccgaagca agacattatc tgaagcaaga agagattatg tcagtgcctc
    58081 aaaaacaatg ggaaccaatg attttaaaat catgttccgc gaaatcatgc cgaatctaag
    58141 ttcattaatc attactaatt tgacgatgaa ctttgcggca aacatcggta tcgagacaac
    58201 tttgactttc ttaggtttcg gcttgccggc aaatgtacct agtttaggta cattgatcgg
    58261 ttatgcaagt aacggagaag tcttagtgaa tcggcaatgg atctggttac ccgcatcaat
    58321 ccttatttta gttttgatgt tgagtatcaa ttatgttgga caagcattca agcgttctgc
    58381 agatgcgcgc caacggttag gctagtatag aagcgaaggg ggaaatagga atgaagaaaa
    58441 cgatttttgg atttgcagcg ctgatgtctg ctgtcgttct tgcagcatgc gggggcggag
    58501 gtacagatac ctctggaaac aactctggag gcaacggcaa taacaaagag gaattaactt
    58561 ttccaattgc aacaaaaaat aatgatgatg caatcgaaga cggcgaatta gaagctgcgg
    58621 tcgcaacgga cagtcagttt aaaggattgt tccagtggga gttttaccaa gattcatatg
    58681 atgcagcttt tatggctcct tctcatgaac cgctatttgc aaacgatgac gatttcagaa
    58741 tcacagacga cggagctgca acgcttgatc tagatgaaga tacgaaaaaa gcgacaatca
    58801 aagtaaaaga caacgtcaaa tggtcagatg gtgaagatgt aacggcggac gatgtgattt
    58861 tcccttacga aatcattggg aataaagatt acactggtat ccgttatgac gacactttcc
    58921 gtaacatcgt aggtatggaa gaatatcatg atggaagtgc cgacacaatc tccggaatca
    58981 aaaaaataga cgacaaaaca gttgaaatcg aatataaaga agtaaaccca agcatgcttc
    59041 aatcgggtgg tggtatctgg tcatatgcag caccaaaaca tacgttagaa ggtgtagcaa
    59101 tcaaagatat ggaatcaagc gatcaagtac gtaaaaatcc agtgactttt ggcccttatt
    59161 acatgagtaa tatcgttgca ggagaatcag tagaatactt gccaaacgag cactactgga
    59221 atggtacgcc taaattgaaa aagatcacga tgaaatctgt accgatggca agtgcaactg
    59281 aagcattgaa ctctaaatta tatgatatga ttttccaaat gccaacagat acttacgata
    59341 cctataaaga aattgctggc tatacaaact taggccgtga acaaacttct tatacttatc
    59401 taggattcaa gttaggtaaa tggaatgcag atacaaagag tgtggaatac aatccagatt
    59461 caaaaatggc tgataaatca ttacgtcaag caatgggcta tgcattagat aataatgcag
    59521 taggtgaacg tttctatgca ggtttgagaa gcaatgcgac ttcattgatt cctccagtat
    59581 tccagtcata ccatgatgat gagatgaaag gattcacaca agatctagac aaagcgaaaa
    59641 aactattaga tgatgctggc tacaaagatg tggatgacga tggtttaaga gaagacaaag
    59701 atggaaataa actaacaatc aactttgctt cgatgtccgg tggggaaaca gcgcaaccat
    59761 tggcagatta ctatatccaa caatggaaag aaattggttt ggatgtccaa tacacaacag
    59821 gtcgtctgat cgaattcaat tctttctatg ataaattaga aaacgatgat cctgaaattg
    59881 atgtttacca agctgcttgg aatacaggaa ctgatccaaa tccaagtgga ctatggggac
    59941 caaatgctgc gttcaactac actcgttatg aatcagaaga aaatacaaaa ttgatcaacg
    60001 atatcagttc aagcaaagca tttgatgaag agtaccaaaa agaagcatat caaaaatggc
    60061 aacaatatgc atttgaagaa gcatttgcga ttccaacgtt gttccgtaat gaagtattgc
    60121 cagtcaacga ccgtgtcaaa gactgggatt ggtcttatga tgtaccaaac ccatgggcaa
    60181 atgtttctgt tacagcagat tcacgttcat aatcactaaa aatgaccgaa aaagggcgat
    60241 gtcctttttc ggttttttga tatttttctg tttatttctc taatattcag gtcaaggcgc
    60301 tgatttcatg acttctccaa taaacggtgt tcaaagtcca gtttctcggt attaagtcaa
    60361 atttatgcaa aaatatggaa agccattttc acaaaatttt ttcttcatac tcgaaactaa
    60421 acgaattttt cacaacttct cgtcaatcac taggattatt aaagctttac ttaccttttg
    60481 accgaaccat ttcaaatttg acaaaaaacc agtataataa ggaagtatcg aaaaaatatc
    60541 cataaaagga atgtggaaaa tggacaacca gctgacaaaa gaactaaaag aaaactataa
    60601 catcgttttt cacgatctaa atttgcttga acaagctttt acccattcat cctatgtgaa
    60661 tgagcatcgc aatctgcaat tatcggataa tgagcgactt gaatttttag gagatgccgt
    60721 tttagaactg atggtttctc agtatttatt ccgtttatat cctcatttgc cagaaggaaa
    60781 attgacaaaa acacgagctg cgatcgttcg cgaagacagc ttgtcgaaat ttgccaaaga
    60841 atgtcacttt gatcaatatg ttttgttggg caagggagaa gagaattcag gaggacgtac
    60901 gcgaccagca ttgctttgcg acctattcga agctttttta ggcgcgttat atcttgacca
    60961 gggattcgac acgacccatg cattcatcga aaaagtgatt tttcctaaag tcaaagcagg
    61021 tgctttttca catgagatgg atcataaaac aaaactacaa gaagtcttgc aaaaatcagg
    61081 tgatgtatca atcgaatacc gattgatcaa tgaagaaggt ccagctcatg aacgagtatt
    61141 ttggatcgaa gtttatgtag atgatcaatt gattggcact ggacaaggga aatcgaaaaa
    61201 attagcagaa caagctgcgg cggaaaatgc attggcagca ctgtaaaagt aagtgaggga
    61261 aagctttgta tttgaaacga attgaaatag ccggttttaa atcatttgca gacaagacag
    61321 tcattgattt tgaaaatagc gtcacagcag tggttggacc aaatggcagc ggaaaaagca
    61381 atattacaga ggctatccgc tgggtattag gagaacaatc tgctaaaagt ctgcgaggcg
    61441 gaaaaatgcc ggatatcatc tttgccggtt ccgatacccg taagcagctg aatattgcag
    61501 aagtcacagt gattttagac aatagtgatc attatctgcc attggagtac aatgagatca
    61561 gtgtaacccg ccgttatcga cggactgggg aaagcgagtt tttcatcaat aaacaaagct
    61621 gcagattaaa agatatccaa gaattgttct tagattctgg attaggaaaa gaatcgttct
    61681 caatcatttc tcaaggaaaa gtagaagcga ttttcagtag taaaccggaa gaccgtcgtg
    61741 ggatctttga agaagcagct ggtgtcttga aatacaagca gcggaaaaag aaagcagaac
    61801 agaagctttt tgaaacagaa gacaacctca gccgcgtcca agacatcatc cacgaattgg
    61861 aagaacagtt aactcctttg gccgcccaga gtgaggcagc aaaggagttt ttacggctaa
    61921 aagaaacact gactcaaacc gatgtctctt tgatggttgc tgaaatcaag acagcaaaaa
    61981 aagattggga caacaaacaa gcacagcttg cgaaattcaa tctagaatta ggcaaactat
    62041 ctgagtcgat ccaagaacaa gaatccattt tggcgaagca gcgtaaagaa aatgcgcaag
    62101 ccgatcgtct gatcgaaaaa aatcagcaag tattattaga tctttcggaa aaactgaagc
    62161 agacagaagg acaaaaagat gttttgcagg agcggacaaa gcatacacaa aaaagctcgc
    62221 aagaatatca aacttcatta gcagaagcac aaaaaaaggt taaacatttt gaaaagcttc
    62281 aagaaagctt gatgaaagca gcagcagaaa aagaaacaga gatccaaaaa gctgaagcaa
    62341 atctcataaa aacacagcaa gaattagaaa aataccaaaa atcaatgaaa gaattattgg
    62401 cagaattaag agatcaatat gttgatttga tgcaagaaca agcagctgta ggaaacgaac
    62461 tgaagtatct agaacgtcaa tatatacaag aaacagcaaa aagcaagcag acgttagcca
    62521 agcaatcgga agtagaagca tcagtagacc gattgatctt acaaaaagaa gaattgacac
    62581 aaaagcaggc gcagctaaaa tcatctttaa ctgaaacaaa agagaagtta gaaatgatcc
    62641 aacaaaacgg gaaaaagttc caagaagcat tagcaaaaga acagcctaaa atgtatcaat
    62701 taatgaatca agtccagcag ctacgtgctc gtaagaaaag ccttcaagaa atccaagaaa
    62761 actattttgg tttttaccaa ggggttcgat tggtcttgca gcataaacag cagctttcgg
    62821 gtattgtcgg agcagtggct gaactgatcg atgttcctgc agatttcacg ttagctatcg
    62881 aaacagcttt aggtggggca gcccagcatg tcatcgttga aaatgaaaaa gatgctcggc
    62941 aagccatcac gtatttgaaa cagcaaagag gaggaagagc aacattcctt ccactgacga
    63001 caatcaaacc aagacaatta ccggctcata tattgacaca agcagcagct gtagaagggt
    63061 ttatagggat tgccagtgaa caagtcagtt atcccgatca aatccaaact gtggttcaaa
    63121 acctgctagg aacgatcctt ttagccaaag acttaactag tgccaatgca atcgcgcaaa
    63181 cgatccgcta tcaatatcga gtggtttcct tagaaggtga tgtgatgaat gccggaggat
    63241 ccatgacagg tggagcgaat aaacgaggaa accaaggcag tttgtttgta caaaatcaag
    63301 aattgaaaca gttgactagt gaatttgaag aagcagataa acaactccaa gcacaagaaa
    63361 aaaaagttca ggagctgcaa caagaaacag ctcgtttagc cgaagaacaa gaagttttgc
    63421 gtacacgagg cgaacaatta cgttttgaag aacaagaagc gacaaatcag ctacaaaaca
    63481 tcataaatga acttgaacgt tttgaaaaag aaaagcaaat ctcaactttt gagacacgtg
    63541 aactgcagca gtttatcgaa gattacgaaa agcaaacgaa cgaattgaaa gacaaacaaa
    63601 cagatcttga atcgcaacga caaaaaatcg acgaagaaat caaatcgtta agtcaagaaa
    63661 gcgaccagat ggaagccaga agagcacaag tacaaagtca aaaagcacaa gaacaagcag
    63721 atcttgctgt cttgaaagag cagtttaatc atctgcagat ccagctccgc ggtgcccgag
    63781 tccaaaaagc agaggcaacc gaacgtcaag aagcgataga aaagcagttg gcgacattga
    63841 cagcagactt ttctgatcat gaagtcacag aagaaagttt ggaaaaacaa attaacgaat
    63901 tatctgctca gcgagaaaca ttaaaagccg agttggcaaa agccaaagag cagcgagata
    63961 gaaggcaaaa ggagatcgat aagcttgaag cagtcttggc agagcgtaat cgtgagcaaa
    64021 aagcacgact gtcggaacaa tccaaattag aagtccaaaa agaccgagct gaaatgattt
    64081 tagataatca tttgacctat ctgcagtcag aatatcagct gacatttgag aaagcttccc
    64141 aagactatca agaaacaacc gatatcgaag acagtagaac aaaggtttca tccttaaaag
    64201 aacagattga aaaactggga ccagtaaact taaattcaat cgaacaatac gaacaagtca
    64261 gtgaaagaca tacattctta gcaacacagc gagatgatct gttagcagcg aaaaaccaat
    64321 tgtttgaaac gatggatgag atggatgatg aagtgcggac aagattcaaa gaagtatttg
    64381 aagctatccg tcaagaattc aaaattgtct ttccaaatat gtttggcggc ggtaaagctg
    64441 aattagtact gacggaacca actgatttgc tgaaaacagg tattgagatt gaagtacaac
    64501 ctcctggcaa aaaactgcaa agtctaagtt tgctttctgg aggagaacga gcacttacag
    64561 ccatcgcgtt gctcttttcc attatccgtg tctgtcctgt tccgttttgt atcttagatg
    64621 aggtagaagc agcgttagat gaagccaacg tgaaacgttt tggtcgttac ttgagcgatt
    64681 tccaagatga cacacaattt atcgtagtca cacaccgcaa aggaacgatg gtcgcagcag
    64741 atgtactgta cggagtgacg atgcaggaat ccggtgtatc aaaaatcgtt tctgtacgga
    64801 tggaagatat caatgaagag gcgaaaatca acgaaacgaa agtgtaggaa cgaaaatgat
    64861 caaattagta gcaatcgatt tggacggtac attattaaat gaacaaaagc tgatttcaga
    64921 tgagaataaa caagcattag cacaagcaaa acagcaaggg gtcaagatcg ttctatgtac
    64981 aggacgcccc ttagctgcga tggcgcatta tctgcaagaa ttaggactgg tagatgaagg
    65041 tgactacagc atcactttca acggcggttt ggtacaaaaa aatgatactg gtgaaatcat
    65101 ggaaaagaaa gtgatggaag tatcagatat ccaacgctta tacaaactgg ctcagcaatt
    65161 ggatctgcca ctagatgttt tgtctgacaa tgtggtttta cagctgccta gtgccccaag
    65221 gaagcaatcc ttgtacaatg tgttgaataa gctgctgcaa ttccaaccag cggatataag
    65281 cgatattaca gatgaatttg tgcttaataa ggcagtaatc gcttatgacc aagaagaatt
    65341 agatcctaaa atcaaagaaa taccagcaga attccatgaa atgtatgaga tcatcaaaac
    65401 aagaagtatg cttttggaat ttatgccaaa aggtgttaca aaagcttatg gtatttcttt
    65461 attggcaaaa gatcttggtt tgaaacaaga agaaatcatg gcgatcggtg atgaggaaaa
    65521 tgatttaccg atgatccaat atgctggact aggcgtagcg atggagaatg ctgtgccttt
    65581 cgtcaaagaa gcagcagatt tcgtgacagc cagcaatgaa gaaaatggcg tagccaaagt
    65641 gatccaaaaa tttgtattag agtaggggga gtcaaatggg attttttgat aaaatcaagc
    65701 gtgcttttac tggcgaagag aaaacagaag aagttgaagt acaggagaag tacgacaagg
    65761 gtttagaaaa gacgcgtaaa acatttggtg aacggatgaa tgagttgttt gccaattttc
    65821 gaacagtcga tgaagacttt tttgaagaat tagaagaaac acttatcggt gcagatgtag
    65881 gttttgaaac agcgatacga attaccgaag cattacgtca agaagtgaaa ctcagaaatg
    65941 cgaaaaaatc ggcagaagtc caaaatgcga tcatcgagaa acttgtggat ctctatgaag
    66001 cggaaggaat caatgaagta aatgaactaa atatccaaga agacggacta acagttattt
    66061 tgtttgtcgg tgttaatggt gtagggaaaa caacaagtat tggtaaactt gcccatcagt
    66121 tcaaaaacga aggtaaaaaa gtattgatgg cagcagcaga taccttccgc gctggagcaa
    66181 tcgaccagtt agttgtctgg ggcgaacgcg ctggtgttga agttgttcgt ggcaatgcag
    66241 gaggcgatcc agcagctgtc gtattcgatg ctttggaaag agcaaagaca gagcaggcag
    66301 atgttttact agttgatact gcaggccgac tgcaaaataa agtgaacttg atgaacgaat
    66361 tagaaaaaat aaaacgagta atccagcggg aattaccaga agcaccccaa gaaacacttc
    66421 ttgtattaga tgcaaccact ggtcaaaacg cgatggttca agcaaaacaa ttcaaagaaa
    66481 caacagatgt gaccggtctt gttttgacaa aattagatgg tacagcaaag ggcggtatcg
    66541 tacttgctat ccgtaatgaa ttgcatttac cagtgaaatt ggtcggtctt ggtgaaggaa
    66601 tcgatgactt ggagccgttt gatccaaatg attttgtcgt tggattgttc aaaggattac
    66661 taaaagaaga ataacccatg tctaaaaagc aggagctgtg acaaaagttg cagctccttg
    66721 tttgaatgat tgtttttaac atagctgtgt ttttgatgcc ttcattttta tacttatttg
    66781 taagcggttc tcaaacaatt tcgttctttt tcttagactt tttgattata attagattca
    66841 tatgaattgg aggtcactta atgatgaatt ttaagcaatt attattgcat gtcaatgcgc
    66901 gcccttttat cgaccaagct cgatttggta tagaacggga aggacagcgg gttgatcttg
    66961 caggaaatct agcaaaaacc gatcatccag caatctttgg cgatcgatcc tatcacccct
    67021 atatccaaac agattttagc gaaacacaaa cagagatgat cacccctgtt accgattcta
    67081 ttcccgaatt atttcagtat ctcgctgctg tttatgatgt gactgctcgt tctataccga
    67141 aagaagagat gatctggcca ttaagtatgc cacctgcctt accggaaaaa gacgaagaga
    67201 tcattattgc aaaattaaaa aatttcgaag atgtcttgta tcgacgttat ttagcaaaag
    67261 aatacgggaa gcgaaaacag atggtaagcg gtattcattt caattttgaa tttggcgatg
    67321 agttgctaag aacattgttc agccatcagg aagaatttca agatttttcc gaattcaaaa
    67381 cagaacttta tttgaaaaca gcgagaaact ttatgcgcta tcgctggatg atcacttatt
    67441 tattcggtgc ttcaccaatg agtgagaaga actacttttt agatgaatca catccgcaag
    67501 aacctgttcg cagtattcgc aacagtgcgc tgggctatac gaatcatcca aatgtgaaag
    67561 tttcctatgc ttctatgaaa cagtatttag cagatatcga gcgaatgatc gaagaaggga
    67621 aactttcgga ggagaaagaa ttttatacac cgcttcgttt ccgaggcgga aagaaagtcg
    67681 cagatttagc aacaacaggt gttcgttata ttgaattgcg gaatatcgat ttgaatcctt
    67741 atgcaagatt gggtatcaat ccagagcaag ttcggttctt acaattattc ctgatgtaca
    67801 tgttatggac agaagaaaaa gaagactgtg accaatgggt agcagaaggt acgactcgaa
    67861 ataacaaagt agcactagaa caaccatcag atcaaacaga atttcatcaa gaaggtagag
    67921 aaattcttga aggaatgaag caaatgctgg ttgaattgga ttggttggat tctctttatt
    67981 tagtagagga agcattgact caaatggatc atcctgaaca aacgttagca gcgaaactct
    68041 atcaagaagc acagctatcg agtcagcaag aagttgctgt ggcattggga catcaatatt
    68101 ataaagaaag ccatgaacgt ccctatcagt tggccggttt tcgcgaaatg gaactttcta
    68161 ctcagatatt tatgttcgat gccatccaaa aaggtgtcca agtcaaagta ttagatgagt
    68221 cggatcaatt tttgcgtctg caattccaag accatgtaga atatgtgaaa aatgcaaaca
    68281 tgacaagtaa ggacagctat atcgtcccac ttatcatgga aaacaaaaca gtaacgaaaa
    68341 aagtcttaaa agaagctggg ttccgggtac caggaggcgc agaattttca tccatggaag
    68401 aagcggtaaa agcttatccg agatttgccg agcaggcatt tgtgatcaaa cctaaatcaa
    68461 ccaattacgg gttaggtatc acgattttca aggaaggagc tagtctggaa gattatcaag
    68521 caggtttagc cattgctttt cgtgaagaca gttcggtttt agtagaagaa tttatgccgg
    68581 gaacggaata tcgtttcttt gtgatcgacg gagaagtcca agccatcatg ttgcgagtcc
    68641 ctgctaatgt tattggtgac agtatccgaa cagtgaaaga acttgttgaa gaaaaaaata
    68701 gtgacccttt gcgtggaacg aatcatcgtg cacctttaga attgatccaa ttaggtgaat
    68761 tagaacaatt gatgttgaaa gaacaagggt tgacgatcga atcagttccc caagccaatc
    68821 aaattgttta tctaagggag aactcaaata tcagtacagg tggtgattcg atcgatatga
    68881 ctgatgaatt ttctgaagct tacaaaaaaa tcgccgtttc tgcggtagaa gcactaggag
    68941 ctaagattag tggtatcgat ttgattatac cagataaaga aatcgatcct acaactgata
    69001 aaaaagcata tgggatcatc gaagcaaatt tcaatccagc catgcatatg catgtttatc
    69061 cttttgcagg aaaaggcaga agattgacga tgaatgtatt gaaattgtta tatcctgaag
    69121 tattttaaaa gaaaaagctt ttgacagatt gtcctatttg atgtcaatct gatcaaaagc
    69181 tttttttgca tgtaaaaatt tatgtgtttt ttcgttttaa aataaacgat cccagaagct
    69241 ttttttcttt tttggttttt ctttttcagc tggcgaagag ttttcttctg ggtatttttt
    69301 ttcgatatca atcacaggtt catttactgc ttcttttgct gttaagagca atccgaattc
    69361 ctcaggattt gcagtgacgt aatcgttgac tacagtgaaa cggctattgt ttttcgttgc
    69421 tagctggata tattggtttt ggacagaaag gggtagagcc ccattcaaga gtatggaagc
    69481 atctggatgt tttttgagtt cttttaaaaa gttttctttg ttttttggtt ggatcatttc
    69541 agcaacagtc atactaagat agcagcgttc cctgaatgtc cctaaatatt tatgctgttc
    69601 ttcagggttc actaaaggtg taccataaag tcccttatcc aaatggcttt ttacatcatc
    69661 ttgtgccata aaaaataccc tccttcttct ctttgtcttt cttacgatta tagcatattc
    69721 ccaataatac ctaaaacata tgaaaaagcg gttgcttttg ttcaacgatt cacttaaaat
    69781 aagaatgaaa atcattctca attagaaagg tggattggat gcgctacgga cgactagtag
    69841 cattattagt tattttaagt agtatttcat tgttcatcgg agtatcgaat gtacaattgt
    69901 cagaactgct ccagttagat gggcaaagct tacaaatctt gctagtgact cgtttgccac
    69961 gaacgatttg tttgatcctt gtgggagcga ccagtagtat ttgtgggttg atcatgcaac
    70021 atttgaccca aaataaattt gtttcaccaa cgactgcggg cacgatggat agtgcgcgtt
    70081 taggtatttt agtggctatg attttcctac ctggtgcatc cttgattgtc cgatccttga
    70141 cagcattttg ctttgctttt gcaggaacat tgcttttttt atcccttacg cgattgttcc
    70201 tccagaaaaa tcaattgatg cttccgttga ttggagtcat gttagggaat atcattggtt
    70261 cagttgctac tttttttgct tatcaattcc agttggtaca aaatatgact tcatggcttc
    70321 aagggaattt tgcgactgtg atacgaggaa attatgagtt attgtatgct gttgttccat
    70381 tgttgctctt ggtttggcta tttgcttacc gatttacgat cattggtctg ggcgaatcgt
    70441 ttgcagtaaa tctgggaata gattaccaga agatgcagtt tttaggcatt ggcttagtat
    70501 cattggctag tgcagtcatg ttgatcatgg taggaagcat tccttttatc ggagtgatca
    70561 ttccgaatct tgtctctaga atatatggag atcaagtgca caaaacaatt ggcgtaactg
    70621 ctttagcagg tagtttgttc ttagtatttt gcgatcttgt cgctcgcgtg ttgatttttc
    70681 cttatgaaat accagtcagt gtgatcgttg gaatcatcgg tggggctatc ttcctttatc
    70741 ttctgataag gggacgacga tatgcttaaa aaaaagccat ctatttttat tggtatgctt
    70801 gttcttgttt tatttgttac aggaatgtac ttgacgatcc atacatatgg aaactggcat
    70861 tttgcatgtg cattgaggag taagaaaatt tttgcattta tccttgtctc gctagctgtc
    70921 agcagtgcca caatcagctt tcagacaatt acgcataacc agtttttgac tccttcagta
    70981 ttgggacttg agtcgttata tgtccttgtc caaacggtcc ttttcttctt tatcggagga
    71041 gtgacgatgc ttcagcagac aggtacagtt tttttcttat tgaacgttgg gctgatggtc
    71101 gggctgagtc tattgttatt tttctttctt ttgaaaaaaa tgagagcgaa tcttttttta
    71161 ttactgatgt cagggatgat tttaggtacg ctttttggta gtgtcagtac ctttttacaa
    71221 gtcatcatgg atccgaatga atatgatttg cttcaaggca gattatttgc aagctttggc
    71281 aatatccaga gtcagtatct tttgcctgct tctttgatca ttatcggctg ctggtacctg
    71341 ttatttcgat atcgacatgt gctagacgtt ttccatctcg gaaatgaaca agcagtaaac
    71401 ctcgggatag acacagatcg ttttgaattt ttgattttat gttgtgtcag tcttttgaca
    71461 gggacatcaa cggctcttgt gggtccagtt acttttttag ggtttattgt cgcaaatatc
    71521 agttatcgtg tcttttctac ttatcgtcat ggtcagctat ttatcggaag ctttctggta
    71581 gctatgctgt ttctgacagg aggacagctc gcagtagagc agattttcgg ctggaataca
    71641 acagtgagtg tcgtcgttga atttgtcgga ggaatttatt ttgtcacaaa attgattcgc
    71701 gaaaggaaga gtatcctatg attgatttac aaaaggtcaa taaactgtac catggaaaat
    71761 cagtggttaa agatgtacag ttaacgattc ctgaaaagaa attgactgct tttatcggtc
    71821 caaatggtgc tggaaagtca acggtgttgt cgatgatcag tcgtttgata ccaaaagata
    71881 caggtgaaat ctatttagac cataacgaag taaaggcttg gaaatccaat gaattagcca
    71941 aaaaattatc gatcctacga caatcgaatg aaataaatgt ccaaatcacc gtgaaagaat
    72001 tagtagaatt tggacgattt ccttatacga aaggaaaatt gacgacaaaa gacgaggaaa
    72061 tcgttcagga atcactggaa caccttgggt tagaagaaat agcggatcaa tcgattgaca
    72121 ctctttcagg cggtcaactc caacgtgcgt atattgcaat ggtgctagcg caagacacgg
    72181 attatatttt actggatgaa cctttgaaca atctagatat gaattttgcg gttcaaatca
    72241 tgcagatttt aaaaaattta gtacaagaaa tcgggaaaac aattgttatt gttcttcatg
    72301 atatcaactt tgctgcaagt tatgcagacg aaattgttgc aatgaaagat ggacagttat
    72361 ttacgcaagg aacgacccaa gaaatgataa aaaaagatgt attggatgaa ttatacgaaa
    72421 tggacatacg gatttgtgag attgaaggcc gcagattctg tctttatttc taaaactaac
    72481 attaggagag aagagatgaa aaaattagta gtaggtatct tgattctagc atcatttggt
    72541 ttagcagcat gtggaaatag caacaacacc agtcaggcag acacaaaaaa aagcagcaca
    72601 caaactgaaa ctacattgac gataacagat agtaatggag accaaatcga ggtgccaaat
    72661 aatccagaaa aagtcgtcgt attcgacaat ggttctttag atacgatgga tgctcttggt
    72721 gttggagata aggtagttgg agcagctaca agcagccttc ctgagtatct ttcatcctat
    72781 aaaaaagtag aatcagcagg tggaataaaa gaaccagact tagaaaaaat taatcagctt
    72841 cagccagatc taatcatcat ttctggccga caaagagatt ttcaaaaaga tctaagtcaa
    72901 attgctccga ccattttcct ttctttagat gcgaaaaatc catgggaatc tttccagcaa
    72961 aatgtcactg ctttagggga gatctttggt aagcaagaag aagctaaaac tcaattagag
    73021 gaattatcta gtgcaatcga tcagacgaaa aagaaagcag aagctactga caaaaaagca
    73081 ctagtcacac ttgtgaatga agggcaatta tcagcctacg gatctggttc tagattcggt
    73141 ttcatccatg atttatttgg ctttgaacaa gcagatgatc aaatcgaagc atccactcac
    73201 ggtcaaagtg tttcttatga atatgtgctg gaaaaaaatc cggatatctt atttgtagta
    73261 gacagaacaa aagctattgg tggagacgac agcaaagatg atatttctgc caatgaactg
    73321 gtagcacaaa cgaatgctgg taaaaatcag cagatcatct cccttgaacc agatgtctgg
    73381 tatttaagcg ggggcggttt agaatcaatg aaactgatga tcgaagatgt gaatcaagca
    73441 tttaaatagc ataaataaca gaggattaaa ctctcatact atgtaacaat aatgtttctt
    73501 acattcttaa atagcaagag cctatcctca aagaagttat caaaaaccat ccgtaaaatc
    73561 gtattgatga ttttacggat ggtttttatt tatttgagaa atcttttttc aacgtgaatt
    73621 cattatctac atcatacggg ttgattctag aatggggagt ggcaaaaaat agtgtgatcc
    73681 attttttcag catcaagatc gcctcctttg atttgaatca aaggtacaaa gcgaaactta
    73741 gataaaactt aaatttagat atttttattt tgtaccgatc tcttctttaa acgatgttcc
    73801 atctgctgct tctcggcaat aagtccacta gactagaaaa tatgagaagc tattttctgt
    73861 ccagcgttct tattactcaa agcaaatcgc agttgtcaca atctcttcta aacgatgttc
    73921 catctgctgc ttctcggcaa taagcccact agactagaaa atatgagaag ctattttctg
    73981 tccagcgttc ttattactca aagcaaatcg cagttgtcac aatctcttct aaacaatgtt
    74041 caatcagctg accttcggta ttagctcaaa gcactgattt cacaacctct atttaggcat
    74101 gtgtctcagc aagcggttga tagttgatga tctgcatttt tcgataaaaa tcggcgggaa
    74161 gcttactcca ccgttttaat aattgataga aataactctc ttctgctttg attttatact
    74221 cgcttaatat caatccaaaa ctattcgttt tttttgaagc taatttttta taaatatcca
    74281 atgctttggt tttatcttca ttgacaaaga gtgtttttac ttcaatataa gaaccctcga
    74341 tattttgtgt ttcttcgata aaatagatgt tttttatata ttccttcata gtgattcctc
    74401 caaagaagtc ttttatttga taagtttact ctaccgaaga gttgttaata atccgtgaac
    74461 aaaaagtaat tctttttcca atagttgtta aaggtttttt attttttata gtattttttg
    74521 ttatttaaga aaggaataag gttaaacaaa taacaggttg ctttttgggt ctttttccgt
    74581 tcaagaaact gaacgaagaa aagggaaagt tgtctctttt gtgataagag aaatcataat
    74641 aaacgaatga cttactttcc aataaattga gaacagtcag aaccagacaa attagttgca
    74701 tctgatgaat aaagtgatta ttctatgaca tgaggtgata cagatgaaat tagaagtgac
    74761 aaaagaagca caagaagtat tgaagaataa attagaagca gacgatcaac ttttattaga
    74821 tatcgaaaac ggtgacggtc catttgcaga aagccaagtc agttgccaat tggatacggc
    74881 atttcgattg atcattgtca aaaaagatac acaaacagat ctaagtcttt attctgtagt
    74941 ggctgataca ccagttggac caatcaaaat caaagacagt gcaatacttt acatggatga
    75001 tccatccaca cttgctttag aaccaactta taatagttta caattaaaag gaccaagcgg
    75061 cttgagaaaa ggcaacttgc aagttgttcg aaaagactag agggataaaa tgggaaaatt
    75121 ggcgatcatc agcgacctac atgtggacat caataaattc ggcgaatcag agctgatgca
    75181 gctttggcaa gttttgcaaa agcacaagat cactcggctt catctagccg gagatacggc
    75241 taataaagta gaccgttgcc tagctgttgt cgaatttttc gtcaataaag gattatctac
    75301 gacatttcat ttcggcaatc acgagttggc agatgtgccc aatgaacaga tgatggacca
    75361 ttttccagac ccacattttt taaatgaacg gtatattgct ttaaatgagc agaccgttct
    75421 tttagggatg aatggctggt acgactatca gttttccgaa ttgaaagatt acgaggcaat
    75481 cgtacgaatg aagcgtttgt attggtatga tcggttcatc cagcgtggga aaacagatct
    75541 cgaggtcaat gcagaaatgc ttgcagctgc aaaaaatttg ctagaccagc tggaagcaaa
    75601 aaaacttgag gtgattttat ccactcattt cgttcctaaa cgggcattta tcatgtatca
    75661 atctgctcct tatgagcgtt ggaacaaatt aaatgcattt ttaggatcag aatcattcgg
    75721 agaattactt gaccgttatt caaatatcaa acaagtagtt tttggccata cgcatcgtcg
    75781 ctttgaagat aaatccatcg gtcacacgat ttacagctgc agaccttttg gttattatta
    75841 tgaatggcag ctgacccgag attttgtctt gaaagaacag ttgctagaca gctatgatcc
    75901 tatgaaatta cgtgtcctct tgcgtgccca tcacccagct tttcaggcct atcaaacaca
    75961 acatttgcaa gaggaatttg aacaagcgat gacaatcgtt ccgtattgag acagcattag
    76021 taatgtgtaa aagaaaatac cttgtaaaac ataaggataa gtaagagcag tagatccatt
    76081 tatcgtggat atgctgcttt ttttgcggaa aataatcagc gaatgtttat ttgatcggat
    76141 gatttgtaac tcgcagagta ctagatgcaa gcgtgtattc tttttttgaa cgataatcac
    76201 aaaaaaaaat ataatataca ttttttattt aattataatt aatattgatt gaagtctagg
    76261 cagtcaagtc acattttgat agataggagg gggacttacg aattatttta aaggctatga
    76321 cagctgtatt tctagattgt gtttgcatca agttgcaata aaaactagaa atcaaatcgg
    76381 gctgataatc atttataact aatcgataca taggaccaaa tataggtcta tgttctttta
    76441 tttcataagt atcgtaaaac agaattttgc tatggattaa tccaatcgac tgatatgttt
    76501 agtaaataaa gaaagaggaa tgtcaatgat gaaaaaagaa atcgtattaa cagcagcaat
    76561 gatgttattc agcatggtag caagtacgac ttttgtttct gcaactgaag tatacccaaa
    76621 ggaatataac acagaaggaa cgattacatt tgaagcagga gatgagggtg taactcctcc
    76681 tgttgatcca gagaatcctg atcccaataa acctgtcgat ccatcagatc ctccaagccc
    76741 tggaacagga ggggcattat cgattgatta tggatcaaaa ttcaaatttg ggactcaaaa
    76801 aatttctact gcggataaaa cgtattatgc agctgctgat gtgatgaacg atgggtctag
    76861 aaaaccgaca tatgtccaag tgacagatag gagaagtacc ttatctgggt ggaaattaag
    76921 tgtctctcaa ccagaacagt ttaaaactgc cagtggggat gaactagttg gagcacagct
    76981 gaaatttacg aaagggcagg ctgtttcttt ggttgatcca acatatacgc cgcaaacggt
    77041 gaattctgaa ttaacgttga caccgggagg aaacaatacg ttagcaatta atgctaaatc
    77101 aggaacaggt gtggggactt gggtctatcg atttggtgca aatgaaaatg aaaatcaaga
    77161 tgcggtacag ctttcggtac ccggtaaatc tgtcaagtta gcgcaacaat attccactaa
    77221 attagtttgg acattagaag atacacctaa caactagttt actgaaaaga actgttaaaa
    77281 ataaatacgt agtcatattg tcttgcgagg taatcgagca aaaattgtca aaggctaggc
    77341 taaatttgtt tggatacgaa tagttcatct aggaatttaa cgatcaggag gaaaagatat
    77401 attgaaaaag aggaggatcg tgctcatttt aagcagtctc ttagtgacta atatctttcc
    77461 gatcataggg tactctttgg aattgaatca gttggagcat ggaaatagaa gagacgaaga
    77521 aagcaaactc acttcgacat tcgacagttt aatagaatcc aaccagacac aagaaagcag
    77581 tgaggattta agtatttcga gtagtggaga gaccactgac aacgtagaag ctgagggttc
    77641 ggacaaacaa ggactagaag atgcaccaat ttctgcggtt gagctgcctg acgatcccaa
    77701 tattatttct atcgataaaa tttttcaaac acctatcggt actagcacgt ccatattgga
    77761 aaatgggaag ttactgcagt tgaacccagc agaaaggtct caaagaggag ctatttggtc
    77821 aaataagaag attagtttac tctcggattt tacatttaaa tcttatttat acttaggaaa
    77881 tcaacgagat agagcgggag atggcatgac ctttacattg actggcgatc ctagaatggc
    77941 tagtaatccc agtcaagtta ttggatcgcc aggtatggga attggctcat attctactcg
    78001 ttctggccaa ccttacattg cgaatgcgct ctcgattgag tttgatacgt ataaaaatag
    78061 tggttcttct gatcgaatgg atcgagagat aactgctgat aatagagggc atggacatgt
    78121 cgcttttgtc acgcctaaac caaataataa taattattct ggagagcata ctggcgtgac
    78181 ggtagctcct acatttcttt ctaatggcac atggcgcttg ttaataatta aatgggacgc
    78241 ggagcaaaga agtcttactt atgatttgga aggtgtcggt cactcttctc ggattatacc
    78301 tgatctcatt aggcagtttg gttctacaga agtaaattgg gggtttactt cttcaacagg
    78361 tacttttttt caagaaaatg ctgtcgctgt cacgcgactt ccatcgaatg tcacatcaac
    78421 agctgatgta aaagtcaacg atagtgatta tggggccatc gctgaagctg gaggaaacga
    78481 taagataact atcagaaata cattgaaggt cgagggggat tttcttgaat ataagaaaat
    78541 gcagactagt atcgacctcc cacaagaatt aactattccc gagcatatct tgatcaatgg
    78601 aaatcaaatt gatgccaaag atatcttgat aaccggaaat cgagtcacgc taaatctgac
    78661 agattatttt attgctggag atacgttaat aatcgaattg gagacaactt tgcaggaagt
    78721 aattcctgaa aaagtactca ctatgcattt tgaatacctt gaaaacggga atttgattga
    78781 gcaatcgaat gaaataaacc taaagatcgc aaagctgaag gagatagata tccatgttta
    78841 ttataaagat aaagacagtc agattgatat tgctgaaatg aaaacaatca ctgggaaaat
    78901 aggagagaag tataaagaaa ctcctattga tattgaaggg tatgtttttg actcggattc
    78961 agcgaataca gaaggcatct tctccgaaaa tacagaggat atctattttt attacagaaa
    79021 aggcaaactt tatctctcaa ctgctcctca atatgtcaat tttggaaaac ataaaatatc
    79081 cagtagacca ttaagtgaat tcggacagtt ttcaggtggg ttgaaaatta tagatgaacg
    79141 ggcttttgga agttggaaat tacaattgaa acaagagaaa gttcttacaa atggagattt
    79201 tgagcttcca gaagctcttt cctttgttac ctctgaaaat acaacggtta tagggacatc
    79261 cgctgttacg attttcgaaa gtgatcaaaa gggtgaaagc gatttgtcct ctcttttaga
    79321 ttctacagct catcgtggaa tcagaataga tattcctgta gaaaaccaaa gaatcggaac
    79381 ctttgagggg aaactatctt ggatacttgc agatgtccca ggaaattaac aggaagagaa
    79441 gaatataaca aaattattga gtttcaagaa gggcacaggt accataggct cttgtgtccc
    79501 acagagacaa gattttgtta tagtcttctc ttacatatga ttaaaggagc gattttcttg
    79561 agaccaaaac aacaattatt tataacactc ctagttattt taggggcact ttttaacaat
    79621 atatcagtgt cagctagtgc atttaatttt tcagtgacgc cgataacttc agagaatcaa
    79681 atcgacaaga gaaaaactta cttcgatctt caacttgatc cggatcagga agtagaagta
    79741 aaagcggaat tgagaaatga tacagaaaaa gaagtgaaaa tcgatatttc tgtgaatagc
    79801 gcaaccacca attcaaatgt gatggttgaa tacggaaaaa acgagattga aaaagacatg
    79861 agcctgatct ttgatctggt agattatgtg agctatccac agatagtgac gctaaaacct
    79921 aaaagtgtcc aaaccgttgc ttttcatgtc aggatgccga atgagcgatt tgatggtgta
    79981 ttggctggtg ggatcacatt ccaagaaatc cagacggaaa aggaccaaac agaaactaag
    80041 gatcaaagct tatcgattga gaatgaatac acctatatta ttgctttgtt gatgcaacag
    80101 acactgaacg aagttgctcc gaaccttact cttcatgaag ttaaaccaga tcaaatcaat
    80161 gcaagaaatg tcatcttagc aaacgttcaa aatgatcaaa aaacctatat caatcaagta
    80221 gttattgaaa caaaaattac aaaaaaagga cattctgaag ttttatatca agaggaaaaa
    80281 gaaggattgc agattgcgcc gaatacaaat ttttcttttc caactgcttt aaatggtcaa
    80341 ccgctaaccc caggtgaata tcaccttaca atgactgttt taggaaatga aaatgaaaat
    80401 ggaaaatttt cgagaaaaaa aggaaatact acgatcaact ataccaatca atgggtattt
    80461 gaaaaagatt ttacaataga cggaaaagta gccaaggaat tgaacacgac ggatgttacg
    80521 attaaaaaag acaataattg gatttatatc ttagtagctt cacttttctt attgtttatt
    80581 ttattatgca tacttttgtt tgttttgaga a
//
LOCUS       DAKBVG010000002        58922 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000002 DAKBVG010000000
VERSION     DAKBVG010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 58922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.002"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(146..1126)
                     /locus_tag="OUD09_000090"
     CDS             complement(146..1126)
                     /locus_tag="OUD09_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HCT9691406.1"
                     /translation="MLFLKKEDIIKSFSMREAIDADKKALSLYSAGKASVPLRTNIDV
                     PKSNGQSLYMPAYVEGGEGALGVKIVSVYPENIKKNLPSVPATMIVLDPETGMVSACL
                     DGTYLTQLRTGAVQGAATELLAREDAKIGALIGTGGQAQSQLEAMLTVRKLEEVRIFD
                     IDFERASQFAEKMMQQFSVTMRPTKTSQECVEGADIITSVTTSKRATFSAEWVKKGAH
                     INGVGAYTPEMCEIPREIIKAADVVIFDTMDGVLKEAGDFISPLQDGYIQRDSYHGEL
                     GQLINEELIGRTSGEQITIFKTVGSAVLDVVVATEIVKKAKENNLGKLIF"
     gene            complement(1139..2017)
                     /locus_tag="OUD09_000091"
     CDS             complement(1139..2017)
                     /locus_tag="OUD09_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCT9691407.1"
                     /translation="MIRNILLTLIVAINLYFIIYFLRDLFKNKQNFKEEPGDMRLLPF
                     TSFIIFFLSTFGVSDFAIGTVLYPKLKWVSMKKLPGTLNTQCVVPVAVMALSYITAIN
                     VGIKTLAVCIICQIIGAYLGPRFVVKLPEKTIKLFVGIGLVIAALLIFAGQMNWIPSN
                     GTASELYGGKLILAGFLLFVYGALNNIGIGSYALTMVTVYLLGLNPVAAFPIMMGACT
                     FSVPIGSVQFIKFNEYSRKITLFTSTFGVLGVLVAVFLVKSLDTYILKWIVILVLLYS
                     AYTMLGSQLKKVTAAN"
     gene            complement(2033..2833)
                     /locus_tag="OUD09_000092"
     CDS             complement(2033..2833)
                     /locus_tag="OUD09_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HCT9691408.1"
                     /translation="METIIQIMKKYDFSEMETRVYTTLLEKGNLTGYEVSKISGVPRS
                     KVYNILEKLLKKNLIVVNKSEPKLYHAISANEFLEKLEKSVKNDLSFLTKNLGMIKEK
                     DEEEMLWKVDGIEYVLDKVEHLVKNAKESLLIQVWHENFTDSLLKALQQAEKRVDKFV
                     LILFSSTHEYDLPLEKYYIHGFETDKLADFGARWINIVADEQEVVFGTINEELQSTDV
                     TWTKNHAMVNLAKEYVKHDAYTLKVIAESPEELKAKYGEGFEGIRKIY"
     gene            complement(3108..3257)
                     /locus_tag="OUD09_000093"
     CDS             complement(3108..3257)
                     /locus_tag="OUD09_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9691409.1"
                     /translation="MKKKVIPYCPKPRNAFERGLFKYFEGKKGQDHKEENQKKKSKKR
                     SEYIF"
     gene            4225..4551
                     /locus_tag="OUD09_000094"
     CDS             4225..4551
                     /locus_tag="OUD09_000094"
                     /inference="COORDINATES: protein motif:HMM:NF013698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCT9691410.1"
                     /translation="MFKIFRKNCCGLDVHKTWIYACIGITDANGRTVYKQARFSSFTK
                     GLNELAEWLAKYNCKDVCLESSGKFWIPVFNILEKTCFVTLAHPKYTKPQKGNKTDRK
                     DAKMDL"
     gene            4532..5467
                     /locus_tag="OUD09_000095"
     CDS             4532..5467
                     /locus_tag="OUD09_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCT9691411.1"
                     /translation="MQKWICDLFMCDMIKPSFIPGLEIRQLRDLMRYRTKLTNMKTSE
                     KNRALNCLTVSNLKLDDVFSDVFGKSSTSIIQYLLEHPGESFDVRPFIHARCKTPVEE
                     IQAAVEGAISVPQAIKLRQCLNHINEIEKHREEINLEIQALAEPFSPVLELLRTIPGL
                     DTQPITAIAILSEIGPDMSVFPTSKHFISWAGCCPRNDRSARRTKSTRISRAGQSLKP
                     LLVQIANALLRSKEHPEFKNRYRRIKANRGHKKAIIAICKMLLTAIWNVLSKVEPYSA
                     KGYLEPKPAKEEKVLTQSQALDLLRKRGYTIIDSA"
     gene            6096..6347
                     /locus_tag="OUD09_000096"
     CDS             6096..6347
                     /locus_tag="OUD09_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HCT9691412.1"
                     /translation="MKSNQKSGFYTFIKAISSVLECDCIIGPYSMLIKMGFPSTESLD
                     SFINDLSKFGRTNTQIVFSTLVSLMVYQFWNVYHALLLK"
     gene            6889..7047
                     /locus_tag="OUD09_000097"
     CDS             6889..7047
                     /locus_tag="OUD09_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCT9691413.1"
                     /translation="MNKKQACEFLGIDNNTLDKWIRVYNFPVIKIDGVVRFDVEDIKD
                     FMNTFKKN"
     gene            7119..7574
                     /locus_tag="OUD09_000098"
                     /pseudo
     CDS             7119..7574
                     /locus_tag="OUD09_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(7624..8796)
                     /locus_tag="OUD09_000099"
     CDS             complement(7624..8796)
                     /locus_tag="OUD09_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HCT9691414.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFRE
                     AVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRL
                     KSTNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
     gene            8898..9677
                     /locus_tag="OUD09_000100"
                     /pseudo
     CDS             8898..9677
                     /locus_tag="OUD09_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(9766..10158)
                     /gene="rpsI"
                     /locus_tag="OUD09_000101"
     CDS             complement(9766..10158)
                     /gene="rpsI"
                     /locus_tag="OUD09_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCT9691415.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(10174..10617)
                     /gene="rplM"
                     /locus_tag="OUD09_000102"
     CDS             complement(10174..10617)
                     /gene="rplM"
                     /locus_tag="OUD09_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCT9691416.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(11136..11603)
                     /locus_tag="OUD09_000103"
     CDS             complement(11136..11603)
                     /locus_tag="OUD09_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HCT9691417.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(11723..13261)
                     /locus_tag="OUD09_000104"
     CDS             complement(11723..13261)
                     /locus_tag="OUD09_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCT9691418.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            13341..14027
                     /locus_tag="OUD09_000105"
     CDS             13341..14027
                     /locus_tag="OUD09_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCT9691419.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(14228..14755)
                     /gene="msrA"
                     /locus_tag="OUD09_000106"
     CDS             complement(14228..14755)
                     /gene="msrA"
                     /locus_tag="OUD09_000106"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCT9691420.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(14839..15516)
                     /gene="pgmB"
                     /locus_tag="OUD09_000107"
     CDS             complement(14839..15516)
                     /gene="pgmB"
                     /locus_tag="OUD09_000107"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCT9691421.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(15513..16643)
                     /locus_tag="OUD09_000108"
     CDS             complement(15513..16643)
                     /locus_tag="OUD09_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCT9691422.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(16643..18826)
                     /locus_tag="OUD09_000109"
     CDS             complement(16643..18826)
                     /locus_tag="OUD09_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HCT9691423.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(18823..19848)
                     /locus_tag="OUD09_000110"
     CDS             complement(18823..19848)
                     /locus_tag="OUD09_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCT9691424.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(19893..20720)
                     /locus_tag="OUD09_000111"
     CDS             complement(19893..20720)
                     /locus_tag="OUD09_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCT9691425.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(20717..21783)
                     /locus_tag="OUD09_000112"
                     /pseudo
     CDS             complement(20717..21783)
                     /locus_tag="OUD09_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
     gene            complement(21809..22645)
                     /locus_tag="OUD09_000113"
     CDS             complement(21809..22645)
                     /locus_tag="OUD09_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCT9691426.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(22642..23511)
                     /locus_tag="OUD09_000114"
     CDS             complement(22642..23511)
                     /locus_tag="OUD09_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCT9691427.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(23581..24864)
                     /locus_tag="OUD09_000115"
     CDS             complement(23581..24864)
                     /locus_tag="OUD09_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HCT9691428.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(24878..26563)
                     /locus_tag="OUD09_000116"
     CDS             complement(24878..26563)
                     /locus_tag="OUD09_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HCT9691429.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            26838..27863
                     /locus_tag="OUD09_000117"
     CDS             26838..27863
                     /locus_tag="OUD09_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCT9691430.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(28458..32132)
                     /gene="rpoC"
                     /locus_tag="OUD09_000118"
     CDS             complement(28458..32132)
                     /gene="rpoC"
                     /locus_tag="OUD09_000118"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCT9691431.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(32190..35807)
                     /gene="rpoB"
                     /locus_tag="OUD09_000119"
     CDS             complement(32190..35807)
                     /gene="rpoB"
                     /locus_tag="OUD09_000119"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCT9691432.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            36535..37503
                     /locus_tag="OUD09_000120"
     CDS             36535..37503
                     /locus_tag="OUD09_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCT9691433.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            37521..38426
                     /locus_tag="OUD09_000121"
     CDS             37521..38426
                     /locus_tag="OUD09_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT9691434.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            38423..39229
                     /locus_tag="OUD09_000122"
     CDS             38423..39229
                     /locus_tag="OUD09_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCT9691435.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            39365..40354
                     /locus_tag="OUD09_000123"
     CDS             39365..40354
                     /locus_tag="OUD09_000123"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HCT9691436.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(40671..41195)
                     /locus_tag="OUD09_000124"
     CDS             complement(40671..41195)
                     /locus_tag="OUD09_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HCT9691437.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            41482..43179
                     /gene="scm"
                     /locus_tag="OUD09_000125"
     CDS             41482..43179
                     /gene="scm"
                     /locus_tag="OUD09_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HCT9691438.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTSTTSDSTDTSASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSS
                     TTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDN
                     RTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(43334..44320)
                     /locus_tag="OUD09_000126"
     CDS             complement(43334..44320)
                     /locus_tag="OUD09_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HCT9691439.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(44343..44909)
                     /locus_tag="OUD09_000127"
     CDS             complement(44343..44909)
                     /locus_tag="OUD09_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HCT9691440.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(44923..45339)
                     /locus_tag="OUD09_000128"
     CDS             complement(44923..45339)
                     /locus_tag="OUD09_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HCT9691441.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(45796..47730)
                     /locus_tag="OUD09_000129"
     CDS             complement(45796..47730)
                     /locus_tag="OUD09_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCT9691442.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            48011..48967
                     /locus_tag="OUD09_000130"
     CDS             48011..48967
                     /locus_tag="OUD09_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HCT9691443.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            49057..50160
                     /locus_tag="OUD09_000131"
     CDS             49057..50160
                     /locus_tag="OUD09_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HCT9691444.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            50173..51126
                     /gene="menA"
                     /locus_tag="OUD09_000132"
     CDS             50173..51126
                     /gene="menA"
                     /locus_tag="OUD09_000132"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCT9691445.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(51940..53286)
                     /gene="gor"
                     /locus_tag="OUD09_000133"
     CDS             complement(51940..53286)
                     /gene="gor"
                     /locus_tag="OUD09_000133"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HCT9691446.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(53420..54673)
                     /locus_tag="OUD09_000134"
     CDS             complement(53420..54673)
                     /locus_tag="OUD09_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCT9691447.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(54679..55602)
                     /locus_tag="OUD09_000135"
     CDS             complement(54679..55602)
                     /locus_tag="OUD09_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCT9691448.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(55823..58315)
                     /locus_tag="OUD09_000136"
     CDS             complement(55823..58315)
                     /locus_tag="OUD09_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HCT9691449.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(58322..58789)
                     /locus_tag="OUD09_000137"
     CDS             complement(58322..58789)
                     /locus_tag="OUD09_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HCT9691450.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT        17061 a        12340 c        10756 g        18765 t
ORIGIN      
        1 ttttagggtc gcttcattac tagaattcaa caagagatgt tttttatttt atttaaatta
       61 tggggtcagt ttccatttga ttacaaactt ctttgttttc tttttttaaa cgcaataata
      121 aaggttttaa tttcagcctt tttaattaga aaatcaattt tcccaaatta ttttctttag
      181 ctttcttaac gatttctgtc gcaactacca catctaatac agccgaacca actgttttga
      241 agatcgtgat ttgttctcca cttgtccgac cgatcaattc ttcattgatc agttggccta
      301 gttccccatg atagctgtct ctttgaatat acccatcttg cagcggtgaa atgaagtccc
      361 cagcttcttt tagaacaccg tccatggtat caaaaataac aacatctgcg gctttgatta
      421 tttctcgtgg aatttcacac atttccggtg tgtaagcacc gactccattg atgtgcgcac
      481 cttttttgac ccactcagca gagaatgtcg cacgttttga agtggtcaca cttgtaatga
      541 tgtctgctcc ttcaacgcat tcttgactgg tttttgttgg gcgcatcgtt acagaaaatt
      601 gttgcatcat tttttcagca aattggctgg cacgctcaaa atcaatatca aaaatccgaa
      661 cttcttctaa tttacgaacg gtcaacattg cttctaattg tgactgtgct tgtccgccgg
      721 ttccaatcag cgcgccaatt ttagcatctt ctcttgcaag aagttctgtt gctgctcctt
      781 gaacagcgcc tgttcttaat tgtgtcaaat aagtaccatc caaacaagcg gataccatac
      841 ccgtttctgg atccaatacg atcatcgttg ctggtacact tggtaagttt ttcttgatgt
      901 tttcaggata cacggaaacg attttgacac ctagcgcgcc ttcgccacct tctacataag
      961 caggcatata taagctttgt ccgttgcttt ttggcacatc gatattggtg cgaagaggca
     1021 cacttgcctt tcctgctgaa tacaatgaca gtgctttttt gtccgcatcg atggcttctc
     1081 tcattgaaaa acttttgata atatcttctt ttttaagaaa taacatttga atcgctcctt
     1141 aattagctgc tgtaactttt tttaattgac tgcccaacat cgtatatgca ctgtatagta
     1201 agaccaaaat cacgatccat ttcaggatat aggtatctaa agatttgact aggaatacag
     1261 cgaccaatac ccccaataca ccgaaggttg aagtaaaaag tgtgattttt ctgctgtatt
     1321 cattaaattt aatgaattga acactgccga tcggtactga gaaggtacaa gcccccatca
     1381 taataggaaa ggctgcaact ggattcaagc ctagcaagta gacagttacc atcgttaacg
     1441 cataagaacc aatgccgata ttgttcaatg caccatagac aaagagtaaa aatcctgcca
     1501 agattaattt tccgccatac aattcactgg ctgttccgtt tgatggaatc cagttcattt
     1561 gtcctgcaaa aatcaacaat gcagcaatga ccaatcctat gccgacaaac agtttgatcg
     1621 ttttctctgg taacttcacg acaaatcttg gtcctaaata tgcaccgata atttggcaga
     1681 taatacaaac agctagtgtc ttgatcccta cgttgatggc agtgatgtaa gacaatgcca
     1741 tgacggctac cggtactaca cattgggtat tcaacgttcc gggtagtttt ttcatgctga
     1801 cccatttcaa ctttggataa aggacggttc cgatagcaaa gtcggaaacg ccaaatgttg
     1861 aaagaaagaa gatgataaat gacgtgaagg gcaacagacg catatcccct ggttcttctt
     1921 tgaaattttg tttgtttttg aaaaggtctc ttagaaagta aataataaaa tatagattga
     1981 ttgcgacaat tagtgtcagc aaaatattcc gaatcataaa aaattcctcc tactagtaaa
     2041 tctttctgat tccctcaaag ccctcaccat attttgcttt aagttcttca ggagattctg
     2101 caatcacttt tagtgtatat gcatcatgtt tgacatattc ttttgctaga ttgaccatgg
     2161 cgtggttttt tgtccaagtg acatcggttg attgcagttc ttcgttaatg gtaccaaaca
     2221 cgacttcttg ctcatcggcg acgatgttga tccatcttgc tccaaagtca gcaagtttgt
     2281 ctgtttcaaa accatgaata taatactttt ctaagggaag atcatattca tgggtagaac
     2341 tgaataaaat cagtacaaac ttatcaactc ttttttcagc ttgctgcaat gcctttagta
     2401 gtgaatccgt gaaattctca tgccaaactt gaatgagcaa actttctttt gcgtttttca
     2461 ccaaatgctc tactttatcc agaacatact cgataccatc aactttccac agcatttctt
     2521 cttcatcttt ttctttgatc atccctagat tcttggtaag aaaagaaaga tcatttttaa
     2581 cacttttttc taatttctct aaaaactcat ttgccgagat ggcatgataa agtttaggtt
     2641 ctgatttatt aacgacaatc aaattctttt ttaatagttt ttccaaaata ttatatactt
     2701 tcgagcgtgg cacacctgag attttactta cttcatatcc tgtaagatta cctttttcta
     2761 atagtgtggt atacactctt gtctccatct ctgaaaaatc atattttttc atgatttgaa
     2821 taatcgtttc cattgattaa tcctcacttt atttttagta gctactacgg taacaactat
     2881 aaattctttt tgattatctg tcaagagtta gttcaataac acacatataa taattagctt
     2941 aaaaacaatc tttttattaa aaagaaacat tataatatga atgtttgtga aaaaataata
     3001 caacttagtc attctttaca acgatttata gaaaaacaac ctctttaaat ataaaaggta
     3061 cgggttactg attacttgta aatctttacg ttccagtttt attctttcta aaaaatatac
     3121 tcacttcttt tcttagactt ctttttttga ttctcctctt tatggtcttg tccttttttg
     3181 ccttcaaaat atttaaataa acccctttca aaagcatttc taggttttgg acaataagga
     3241 atgacttttt tcttcatata aatacctcat ttaactattt gatatcatca cattatcaaa
     3301 aaaaataggt tttttattaa aactatcttg acctttcggg agatttcact gtgatttatt
     3361 tttattccca tacttttttc gttggtcact ttcgtatacc taatctaagc gatcatcggc
     3421 gacggtgctt ttacaacgaa tggcaggaca aaacaagggg cggaaaaact tttttgccta
     3481 tttcataaaa aactttttcc tacagcaatc ccttgttttg tcctaccaca cgttgtgaac
     3541 ccaaagcacg ccgacgaacg cttagctata cgaaaatgac ctccaaaaac aaaaaagtga
     3601 ggtgaataga aacaaaaaga aattgaaatc tcccaaaatg tcgtccgcct cggagacaac
     3661 ttgctgctga aaatggttgg ttaattcatt taagtattaa ataagataaa tcatcaaatt
     3721 aatcggaaaa atagcatgat tggccttatc aaaaataaac tgtaaaaact tatatttcta
     3781 tgagtggtgg aacaatgcta ataccaaatt aatttatcga ttacccacct gcacaaacca
     3841 aagatagcat tattaggaaa cgaatcaatc agcgaatcca gcattataat gaggatacta
     3901 aaaataaagt tttcataatt ataaatccgc cttcctacga aaggcaccaa tgaggtgatg
     3961 aaaaccttag agcgctttct ttcttttttt attccgtgtt accctttcct tgcaggtccc
     4021 tggtacacta cagaacccga ggaggtgagt ggcggggctg tatagcggca accccgcata
     4081 attatatggt gtcggtcatc cgttaaggca tcaatgaatg gcgcaaaccc gtagcccgta
     4141 aacttatctc ttccgaagtt gactcgattt cgccggatga ccagtcccta ccaaacctgt
     4201 actatttttt tacaggaggt tgctatgttc aaaatctttc gtaaaaattg ttgtggcttg
     4261 gatgtccaca aaacgtggat ctatgcgtgt atcggaatca cagatgcgaa tggtcgtaca
     4321 gtgtataagc aagctcgctt ctcttccttt acaaaaggtc tcaatgaatt agctgaatgg
     4381 cttgctaaat acaactgtaa ggatgtgtgt ttggaatctt ccggtaaatt ttggattcct
     4441 gtttttaata ttcttgaaaa aacttgtttt gtcacactgg ctcatccgaa gtacaccaaa
     4501 ccccagaaag gaaataaaac agatcgcaaa gatgcaaaaa tggatttgtg atctctttat
     4561 gtgcgacatg atcaaaccca gttttattcc tggtcttgaa atccgccagc ttcgtgatct
     4621 aatgcgttat cggacgaaac tgaccaacat gaaaacaagt gaaaagaatc gtgctttaaa
     4681 ctgcttaacc gtttctaatc tgaaactaga tgatgttttt agtgatgtgt tcggaaaatc
     4741 atccacttct atcatccaat acttgttaga acatcccggt gaatcatttg atgttcgacc
     4801 atttatccat gcccgttgta aaactcccgt tgaagaaatc caagcggcag ttgagggggc
     4861 gatttctgta cctcaagcga ttaagcttcg ccaatgcctc aaccatatta acgagattga
     4921 gaagcatcga gaagagatca atctagagat tcaagctctt gcagaaccct tctcccctgt
     4981 tttggaacta cttcgtacca taccgggctt agacactcag ccaataacag cgattgcgat
     5041 tctttcagaa ataggaccgg atatgtccgt gtttccaact tcgaaacact ttatttcttg
     5101 ggctggatgc tgtccaagaa acgatcgcag tgcccgacga acaaaatcaa cacgtatctc
     5161 tcgtgcaggg cagtcgttga aaccgctact tgtacagatt gccaatgctc ttctcagatc
     5221 caaagaacat ccagaattta aaaatcgcta tcgtagaatc aaagcgaatc gtggacacaa
     5281 gaaagctatc attgctattt gtaagatgct cctgaccgct atctggaatg tcttatcaaa
     5341 ggtggaaccc tattctgcta aaggatatct ggagccaaaa cctgcgaaag aagagaaagt
     5401 tctgacgcaa tcccaagctc tggacctttt aagaaaacgc ggatatacca ttatagatag
     5461 tgcttaaacc aaagttacgt tgattcttcc taaatagact cgttgtcttc catttcaaaa
     5521 attttgaaaa cggtaggctt atttagtgta ccgttcttca ggtatcttca aatagattta
     5581 ttttcaaact tatttccttt cttcttttac tctcctatag cttagcattt aagtgggcac
     5641 tcgtgtcacc ttgctagata gctatgaatg ccgtaataag cctcctagta tataggcaga
     5701 ctgccaatta taactgattc aatccacatt atgataaaat ccaccttttc gtttcatatg
     5761 tgttgaaata tcacatatgt caaaagtgca cgagtctttt cattcagtgc tttgtaaata
     5821 ctcaaaatta ttccttttca ctttaaaaaa aagataatta gtacaaaatc agaaatgagg
     5881 aaagtattag gatggactag aaaggagttg ttgaatttgt tgcaacagat tggtcattta
     5941 tcaattaaaa attaggtgaa gttttattca tcactgcagc tgctgtttcc cagagaataa
     6001 aacgtctaga agaaactggt cagttactgt attatcaagc agcaattgac tacgaacaag
     6061 cttgattact aaccaaagcg tttatccacc tgttgattaa atcaaatcaa aaatcaggtt
     6121 tttatacgtt cataaaagca atctccagtg tactcgaatg tgactgtatc ataggtcctt
     6181 attcgatgtt aatcaaaatg gggtttcctt ctaccgaatc attggacagt tttatcaatg
     6241 atttatcaaa gtttggacga accaacaccc aaatcgtttt ctcgactctt gtttccttaa
     6301 tggtttatca gttctggaat gtttatcatg cgcttttatt aaaatagaaa agagcttttt
     6361 tcatgcgaca aagcagtgat catttattgg cacatgcaat ctttgtggat ccattttttg
     6421 aaaatacgat atttagtttt gacccaaaaa tctttaaata cgtatttgat aatttatctt
     6481 aacaaccaga taattttatt cagcaccgta ttatctatat tgaattctcg gaatcaagat
     6541 aaattttttt ccgtttttgt ttctttttta atcttcttct tgttattaat agtgattagg
     6601 atgatattcc tatattgatc tttgataatt agatataatt tttgaataac gttccctgct
     6661 attcgagcta gaaatacaag tacgccatga cctagttagc gacaactact tagagatttc
     6721 ttttgttctg ggacaacaaa atgccatgac aatagcagta aataatgata cttccgtaag
     6781 gctcgccgta aactggggag aggtgaaatt cctatgtgct tacccaaagc ggttcaaaaa
     6841 ttttaaacta ttattttagg agatgatttt ataaaatcga aagcctactt gaataaaaaa
     6901 caagcctgtg aatttctagg aattgataat aatactttag ataagtggat tcgtgtatat
     6961 aattttccag taataaaaat tgatggcgta gttcgctttg acgtagagga tatcaaagat
     7021 ttcatgaata cattcaaaaa aaattgaaat tttcttctcg tcacatatac tataaatagc
     7081 gaaatgtact gccttacttt ttaaaggagt gaggaagttt gcctacaaag ctttcaaatg
     7141 ggaaatataa aactaatctg aggtatccaa aaaaattcag agaaatcact ggaattgcat
     7201 cagagaagta tcaaaaagta tttccaacta ggcaattagc tattaaggcc gaaagggaaa
     7261 tgaaaaagaa gattgaaaca gttttaagag aggagaatgc aaactcacta gaattgaaag
     7321 gaaaaataaa atttaaagat ttttatgaat caaaatggct tcctagatat gaactaggtc
     7381 aaactactag gtcaaacagg gctccaagta atgtaacaat tagtaatact aaggatatat
     7441 tccgattgca catccttcca atgtttggtg aatatgcaat gaattatctg aattcaaata
     7501 ctgagataat atcagatgaa ttaacaaaga aatcgaaaga atacgcaaat ataaaaatta
     7561 tcaaaggcta tgtagtcaag tccagactcc tgtgtaaaat gctatacaat gtttttacca
     7621 tttctactta tcaaaattga tgtattttct tgaagaataa atccattcat catgtaggtc
     7681 cataagaacg gctccaatta agcgattggc tgatgtttga ttggggaaga tgcgaataat
     7741 cttttctctt ctgcgtactt cttgattcag tcgttcaatt agattggtac tctttagtcg
     7801 attgtgggaa tttccttgta cggtatattg aaaggcgtct tcgaatccat catccaatga
     7861 tgcgcaagct tttgaatatt ttggttgatc gatataatca tgaatcaatc gatttttagc
     7921 ctcacgcgct aagttaatat ctgtgaactt aaaaattcct ttaacagctt ctctgaaaga
     7981 ttttgaattt tttttaggaa tggtggtaaa gatatttctt aggaagtgaa cttggcatct
     8041 ttgccaactt acgttggtga aggattttct aatggcagag actaatcctt tgtgcgcatc
     8101 agaaataacg agttccgtac cttgtaaacc gcgttctttt aggtattcaa aaaatgttgt
     8161 ccaggtctct tcgctttcgc cactttgaat catgaagccg ataatttcac ggtcgccatc
     8221 tttggttatt ccaatcgcta tatgacagct ttttgagagt actcgatttt cttctcgtac
     8281 ttttatatag agtacatcgg tcattaagta aggataattt ttttctgata ataaacgatt
     8341 ctgccactcg ttaaccatag gttctagctg ttctgttaag ctagaaacga aggacttaga
     8401 gacggattta ccacaaagtt cttccacaat ttttgatact ttacgagttg aaacgcctga
     8461 tacatacatt tccaacattg aagccatgag ggctttttcg tttcgttgat aacgttcaaa
     8521 cactgtgggt gaaaaatggc catcacgtgt tctgggtact tttaattcta gcgtgcctac
     8581 acgtgtcgta aagctgcgct cataatagcc atttcgttga ctttgtcggt tttctgttcg
     8641 ttcatattct tttgcttgaa tatattctgt tcgttgattt tccattagtt gattaaatac
     8701 cgttgttaaa atatttttag aaacgtcatc ctttacagaa tattcaataa tgctttgaat
     8761 ctcttcgctt ttcagtgtaa aatgtacttg ggtcatgtaa aagtcctcct gggtatgttt
     8821 ttgtcgttaa aaacattgta ccgtaaaagg actgttatat ggccttttta cttttacaca
     8881 attatacgga ctttatcggc tatgttcgct ctatgtttga tattgccgaa atactatgtt
     8941 atatcgaatt caaccgaact accaaaatta ttcaatgtat taccgcgcct aagaaaatgg
     9001 ctttagaaga aaaaaggata agggaaggga atcaagcatt atcatcagaa gagctgactg
     9061 attggataga agcagtaaac aatgacttaa ataatcactt gcttacactt cacgattaca
     9121 cacttttcat gcttacctta tatctaggtg atagaaaaag tgagacttat gctctgcaat
     9181 ggaaatatat tgattttaat aaaaatacgg ttcgtctcaa atatgcttta gataaatatc
     9241 aaagaaagac acatacaaaa ggttggaaag acaccgttat tcaggcacca gaagttgtga
     9301 tgacattatt aagagaatgg aaatcagctc aggctgaaca attgttgaaa ctaaaaatta
     9361 tacaaacacc tgaccagtac ctctttacct attccaaacc ttctggagaa gtaaattgtc
     9421 ctgttcacgc tgactactta aactatcgaa tcaatgcaat aaaaaaaagg catcctgaat
     9481 tggctcaatt atctccacac aaacttcgtc atacctatgc aactatagct agggaaggtg
     9541 gagcaaatat gaatcaaatc tctaatgctt taacgcactc ggatatttct actacaaaaa
     9601 tatacgtgaa cacacctgac gtagttgata aatctgtttt tgaagcattt caaaaaggat
     9661 tgaataagtg tgattaacat gtgttctcaa atgtgttctt attataaaaa aagacacttt
     9721 ccaaataatt gaaaagtgtc ctgaaatatt gatataaaaa gaatattaac gttttgagaa
     9781 ttgtgaagct ttacgagctt ttttaagacc tggtttttta cgttcgacca tacgtgcgtc
     9841 acgagtaagt aagccagcac gtttaagagc taaacggaag tcaggatcaa cttctagcaa
     9901 tgcacgagcg ataccatgac ggatagctcc tgattgtcca gcgtatcctc cgccgtttac
     9961 gttaacaaat acgtcatatg cgccttttgt ttcagtaaca gcgaaaggtt gattgattac
    10021 ttcacgcaag tcagcatgtg gaatgtattc ttcaacatct tttttattta cagtaatttt
    10081 gccagttcct ggtactagac gtacgcgagc aactgcgttt ttacgacggc ctgtgccgat
    10141 atattgaact tgtgccaatg aaattccctc ctattagatt aagtttgtga tgtctaaaac
    10201 ttctggttgt tgtgctgcat gtggatgttc tgctccaccg tatacgttca attttgtaaa
    10261 ttgtttgcgg cctaaagtgt ttttaggaag catacctttt acagaagttt cgatcaaacg
    10321 acgagaattt ttagcacgca attcgccagc agtgattgat ttcaatcctc ctgggtagtt
    10381 gctgtgacgg taataaattt tgtcagtcgc ttttttacct gttaatttca ctttgtctgc
    10441 attgatgacg atgacgaaat cgccagtatc caaatgaggt gtgaaagttg gtttattttt
    10501 accacgtagt acagatgcaa caactgttga aagacgtccc aatggaacat cagttgcgtc
    10561 tactacatac catttacgtt ctacttcgcc tggtttggcc atatatgttg tacgcacgtt
    10621 attttcctcc aatttaagtt ctgatgtttg acatacactt cgagtttccg gggctctttg
    10681 tggggcaaac aataccattt aacatattac ccgctaatct agtcaatgtc aacgtttttc
    10741 tgcttgattg aaaaatttat atgaaacttt acttcttttc tttcttttat ataaaagggt
    10801 cgtttcctgt caaaatgaaa aacaaaaaga ggctgtgaca aaagtcttgt cacaacccct
    10861 tattttgaat aaacggtgtt ctcctgccaa ctcctcggtc acaagtcaca atatccataa
    10921 aacataagaa gctgtttttt aaaattgttt cttgtgactc ggagctgaac ggcaggctta
    10981 caacctcttg gtaaacggtg ttcaatcagc tgaccctcgg tattagctca aaaaacggca
    11041 aaatatgagg aactattttg acgtttgttt ctctaatact caggtcaaaa cgctgatttc
    11101 acaacctctt tttcggtcaa gttcaacaac agtttttacg cgtcgatctc tggcgctttg
    11161 cccaaatgag cttggatcat ccagatacgt ttttctgtgg ctgttttgaa ttcgataaag
    11221 atatcttgtg ttggatcgtc gccttcttct ccagaaactt cgattccttt ttggtaaaga
    11281 tctgctagat aacgatagcc tgcaacaagt gtttccaaac gttctggaat agtacgatcc
    11341 cagcggccaa tttcatcagg gatttttgtg ttatctgcaa attcacgcaa tgtagaataa
    11401 ggtgaaccgt ctaatgtgat caagcgttcg gatacttcat ctaactgagc gttcaaatca
    11461 tccatgaact catccatcat tgggtgcaat gtaaggaaac ctggtcctct catgtaccaa
    11521 tgtgtttggt gaacaacaac agacatttgg cttagatctg caactaattg atttaatact
    11581 tctttggttt gttggaattt catctttctt gcctcctaac attttcttac acttaaaata
    11641 atagtagact ttcaatgaaa agtacaacaa aatcatttac ctgagaacga ttttcagttc
    11701 gcttttttat ccgtaaagcc aatcattttt cagaatcagc taattcttct cgcagttgag
    11761 caaattgctg ataagtctgt gctaaatgct tttgtgtcga aacaaataat ggatagattt
    11821 tctgatattt tttatgatca gtttcttgta ctccaacttg ctctttcaat tctatcaatt
    11881 cctgtgcttc actgatactt tggatctttc cgatgcttct ccagcctaaa atcacagctc
    11941 ctaatgcgga tgcttctgtg acagtaggga aaacaagcgg gtaacccagt acatcagcca
    12001 tcatctgacg aaaaatcgga gattgagcaa acccgcctgt agcacggatc tctgtgagct
    12061 gattttcctg gcaaactcct tttaaaatcc gataaaggtt gagacagatc ccttctagaa
    12121 tcgccttcat catttctttt tctgtatgtt gccgctgtaa accgataaaa ctgccaaacg
    12181 catctgcgtc ccaaagcggt gctcgctcac caagtaaata gggatgaaaa atcagaccat
    12241 tggcacccgc tggcacaaaa gcgatagccg aaagcagtgc atcatatgga ttttctcctt
    12301 tttctttagc agattgaacg acttcctgta aaaatgtatg ccctgcccaa tcaaagatgc
    12361 cagcaccatt gctagtcgct cctcccacta cccaatgttt ctgatccaga acataacaaa
    12421 acgtctccat ttctggatgg aactgaggct gagtagtaat aaaacgaagt gctccgctcg
    12481 ttcctacggt caaagcggcg atcgtatcac caaaagcacc taaccctaga ttagaaagcg
    12541 gcccgtctgc ccctcccaaa acaaatgata tatcatttgg caaacctagt tctgctgctg
    12601 tttttgatgg tagacctgtt acttgatagg tggaaggaaa gacttgcggc aattgctcta
    12661 ttcgaatgtt caagtagtca agcacttctt ctgcccattg gaattcatgg atatcaaaat
    12721 atccagttcc tgaagctgtt ccgtagtcgc tgatcagttc gccaaacaat ctataaaaga
    12781 tatagtcttt gataccgaca aaataagcag tttttttgaa taattcaggt tggctgtttt
    12841 gcagccactt gattttgttc aatggtgtca ttggatgaat cggcgtccca gtctgccgat
    12901 aaaattcttt gccaagcacg gtatatttca gtttttctgc ttcgtccgct gctcggttat
    12961 ctgcccaagt gatcattcga gataacggtg ttttctcttc atccaacaat atcaggccat
    13021 gcatcgcaga ggagaaggag acaaattgaa tgattgtgtc tgaaggcaac tgtttaatta
    13081 cttcttttat aacggtgcag acaccttgaa agatcaattc ctgatcttgt tctgccatcc
    13141 cttgtgtttc ttggatcaaa gggtagcgaa aataagcgga tgctgcttct tgggcttcat
    13201 ctgtgaaagc aactgctttg gtttgtgtgg ttccgatatc taatccgatt gaaacgttca
    13261 ttttttcacc tcaaatttta tgtatgtaca aaaatttagt ttaagcttgc gtatcttggt
    13321 tttgaaaagg gggagatggc gtgttacttt attttatcat tggttgctgt atcggatcat
    13381 ttttatgttt aaccgcccag cggattccgt taggtcactc tattatttat cctagatctc
    13441 attgtgtcaa atgctgtcgt tcgctttctt ggtatgaatt gattccgatt ctctcgatca
    13501 tcgtccaacg atttcgctgc cgctattgca ggtgtcggtt gcccttctac tatttgttgg
    13561 cagaagcctt atgtggtggt ctttttgcct ggttctttac tttttcttcc gcaagaaatt
    13621 tttcgacgtt catatggatg ctttcggctt tgctattttc gttgatggat ttattttact
    13681 tcatggtcca ttctcacacg ctattctgtt cttgggcaat tttatggatt ttttggttgc
    13741 aggccggcgt cttccaatgg caaagtgtgc tgttaacgtt tatcgtaggc ggtgtgtgcc
    13801 ttcgctatgg tcaatcctat ctcggagctg gcgatactct gttgatgctt tcttggagtg
    13861 gtggcttgtc tctagaagag ttgcttcaga tcttgtttct tgctagtctg tttggaatat
    13921 ccgttttttc tgtttacttt atctgtcata aaaaaaagtt ggaaaagctg cctttcattc
    13981 cttttttgag cagtgcgttg ctgattgtcc ttcatcttag gtattgaaaa aaggttgtga
    14041 caaacgactt gtcacaacct ctgatatata gtgttctctt accaaatcct tgtgacttgg
    14101 agctaggcga caggttcaca acctctccac taaatggtgt tcaatcagct gaccctcagt
    14161 attagctcaa aatgctggtt ttacaacctc tgatatacgg tgttccataa gtagcttttt
    14221 cggcttctta tttcttgacg ccccaatgtc gacgaaggaa acctgcacgt ccagaacctt
    14281 ctcgataaag attgtaatgg agcttgtttt tcttgtaata atcctgatgg taatcttctg
    14341 caggataaaa ttcgcttctt ggttcgattt ttgtcacgat tggcttatcg aatcgcccgc
    14401 tgtcttgcaa tgcttgtttt gaacgctctg caatatttcg ttcttcttca tcattatagt
    14461 agatcactgg acgataagaa tcaccgcgat cagcgaactg tcctaatgcg tcagtcggat
    14521 cggtttgctg ccaatagatt tccaccaact gatcataaga aataacttct ggatcatatt
    14581 cgatctctac tgcttctgta tgccctgttg tgcctgttgt tacttgttca taagttggat
    14641 ttggtacatg cccgccagta taaccggatg tcacagaaat gatccctggc tgctcttcaa
    14701 atggtttgac catacaccaa aagcatccgc ctgcgaaaac tgcttttttt gtcatataaa
    14761 atcccccttt tttctcatca taccttgatt tccaaaagga ggataacgtt ttgcttgtga
    14821 cagaaaaaac ggaaactatt atatacgtga tacggcttct gataaatgcc aaaacgaacg
    14881 tacttgatca aaaacggtta aatcaggaat cacatattca gcagaaggaa gatttatgtt
    14941 ttcacctatt cccaatacat gcatgttacc agccaaagct gcttgacaac cagcttctga
    15001 atcttcaatg accaagcatg catttggcgg tacattcaat tgctgtgctc ctttgagaaa
    15061 aacttctgga tcaggttttg cttttgatac ttgcgtgccg tcaataagga catcaaaata
    15121 acttgtcaaa ttcgtttttt ctaaaatgag gcgtgcattt ttgcttgctg atcccaatcc
    15181 gatcttgatg tttgtttttt tcaagtactc aagaacagct attgcaccag gcaaaacgga
    15241 tgtttcatcc attttttgaa tcgcttgaag atatagattg tttttttgct cagcaaaggc
    15301 tgatttctct ttttctgaga atttctgttc catttttcca aatgacagca atcgctctag
    15361 tgactcggtt ctggatattc ctttcagttg ctcgttttct ttctcagtga atggaatgga
    15421 cagctggtta gctaaaacca gccacgcttc gtaatgataa cgagctgtgt ctacaagaac
    15481 gccatctaaa tcgaatagtg ctgctttgat tttcatcgat tacctccttg aaaatgaacg
    15541 acttgaagta aagatgcgat gtttttagct gtgcgatgaa gattatcagt ggcttgctgt
    15601 aaagcttctt gtaaaggcac agctgcagga aggatcggaa aaacggccga aatgccaaca
    15661 tctgctgctt tttcgcttcc tttaccaaca cttccgcaaa tagctatcac aggaacgccc
    15721 tctggtactc ttctagcaat cccaactggc gcttttcctt ttagactttg tccatctaga
    15781 cgtccttcgc ctacgattac gaggtctgct tgctgtaccc gtttatcgaa ctccagtaaa
    15841 tccaatacgt agtcgatgcc agagactatt ttccctgaag caaatgctaa cagtcctgca
    15901 cccattccac caccagcact actgcccgat gcctcgatta cttgaggggc aaatctcata
    15961 taaaaatcat gcatggcttg gtcagccact gctaattcat tttctttcaa gcctttttgt
    16021 ggtccgaaaa caaaagaagc gccttctttt ccgcataaaa gattagtaac atcagtaatg
    16081 acagtgattt tggctttaat cagctcaata ggcacgttcg ttttatcaac tgtagcaatt
    16141 ttacctagat tttctcccag tgcttctact ttttgattat cttgatcgta aaaagcataa
    16201 cctagacctt ctgccatgcc gatgccgcca tcgttggtac tacttccgcc aacgccgatg
    16261 atgatgtgtc gaacaccttg ttgcaacaat tgtcgtatca gttctcctac tcctcgcgtg
    16321 gataacctaa gtggctgttg ttggtttagt gggacatctt tcaatccgac gatttctgcc
    16381 atttcaaaaa tcgcagtttg accgtctgaa gcgtactgga tttctttgtt tttgccaaat
    16441 gcattagtaa cgactgctgt ttgccgcttc aaccttaaac tgtctgctaa tgatgaaacc
    16501 gttccttctc ctccatcgcc tataggtagc agatcatatt cagcctgaac aaaaacttct
    16561 tgaaaacctg attgaatggc ttgtgctgcc tctaaggctg tcagactttc tttgaaagaa
    16621 tctggcgcaa tcagaatacg cattattttc ccctccgttc caagatcagt tctttttcca
    16681 gctggtattg gtctccattg acaaaaatag gtaatgtttc tttcgtctct ttttttatta
    16741 ctatttgttt ttttgtcaaa acaaatagca acctgtgatt tttccaaata aaaggaaaag
    16801 aaattttttc ccatgctgaa ggaagtcttg gagaaataga aagagttcca ttcgttactg
    16861 taatacctga aaaaccaaat ataactgcta gccatagtgc tcctaatgaa gcagcatgaa
    16921 ttccgtcatc tgatgaatgc ggatctggtc ctaaatcaat caggcacgct tcttggaata
    16981 aatgatacgc ccattccatt tcttggatac ggcaagcctc tatggcgtga atcgctttac
    17041 tcaacgatga gtcgtggatt gttttgtttt catagtaatg caaattctta gaaacgattg
    17101 gttgagaaaa atactcaggt aataagtata acagcatgac aagatctgct tgcttgctga
    17161 cttggagttc atttacttcg ctacgggcat aatcaagtaa aatagactgg ctgccaggac
    17221 gttctttgta agtggtcaaa tcaataactg gcttactgag atacgtgtca tcttgcggaa
    17281 tcagctgttc tttgttaggc actggcaaat ataacttatc taaaaaattt tgacattctt
    17341 tcaaaaaaag cggatcgcca gcttgatatt taacagctaa ggccacatta taatgcgcca
    17401 gatagttcgt atatgcatta ttgtcgatat attctgtgta ttcatctgga ccaatgactc
    17461 tgtgtatctc taaatgatca tcgactttta cagctctgct cagccaaaaa gctgctgttt
    17521 catgaatgag cgtttttccc tttgtttcta aaaacgattg atcctctgtt gcttggacat
    17581 actcttgaac ggcataagca aggtctgcac tgatatgatg ttctgcggca gctgaagcaa
    17641 cttcttgtct tgttccggtt ttgatattca atgcagcaaa tttgggtgtc tcttcctgac
    17701 cagtgtgtgc gctttcccat gggaaaagtg caccttgata gtgataatct cttgcttttt
    17761 gccgtgcgcc ttctaatcga tcataacggt acagcagcaa attctttgct acttcaggaa
    17821 actgatataa aaagaaaggc aggataaaaa tttccgtatc ccagaaaaca tgccctttat
    17881 aaccttcgcc agttagtccc ttcgccccta cactaacact tggatcattg cggggagtca
    17941 taatctgtaa atggtaagca gcgaagtcca tcgctaactg atcgaaagaa gaatcgcctt
    18001 ggatcgttat ccgatgctga tgccaaaaat ctgtccaata agacatcgac tgctctaaaa
    18061 tttctgtata actaacgctt ttctgggctt gggcaatact tttcttaaac gggtctgttt
    18121 gctgaagaga ggtagagagc acgatgatct tctccagcgt aaaggacgta ttttttctga
    18181 tcttttctga aaaggaagcg gtgatccggc gattttttgc ttggaataca cctgctttat
    18241 tgaatttagc tgcaatccca atatcaattt tactttctgt tgtctggtag gatgcactga
    18301 tcaaatcgtc accaaatact cgcagttctt tttcaaccaa ctgctgtgta ccaaaattgg
    18361 tttgctgtcc atcgataccc gttttcacat ctacttggca gtcttcggtc aacggcttga
    18421 tgaccacttt cagggctgcc tgatggagat tattttgatc agcaacacga caagtaatga
    18481 cttccacttt ttttccttga gcagtttcaa tgagcgcata gcgtttaagc tctcccattt
    18541 ctaaatctag tgcccgctcg tatgcaagaa ctttcatcgt ctcaagagaa aaatgttcac
    18601 cgtcgatttt tatttcccat ctgatgacat ctgggagatt cactaactcg gctgattctg
    18661 ctcctaaagg tcggttatat acaccagctg caaacattcc ccttacttga ttccgatagt
    18721 cttcctcctg gcttcctctg ataccaaaaa atccatttcc aatggtcatc tgactcgcat
    18781 acttattgat atactctaaa tcaaactgtt tttcttctac tttcataagg taataacttc
    18841 tcctgtttct gccgaacgat ataaattttc caccagattc tggatgacat atccttgcca
    18901 gccatctgca atcatctgag tctggtcacc gagcgcccga ttcacaaaat gagccatact
    18961 tagttcatgt gggtcattaa ctggcgtggt cttttgtaag gaaaccagct ttccatctac
    19021 atcttcgaat agttcgcctg gatacagatt agcacctgcc tgatcgccac aaaaagccac
    19081 attcagtttg tcttctctga tattcaaagc aaacgaagta ttcagttgga tgatcttgcc
    19141 gttttctaat tccagtgtgc caaaaattgc atcttctact gtgaatttgc ttggatccca
    19201 tttcccgaat ttccctgaat tttttttcgt accaattttt tggaagctgt acgcccgaac
    19261 gctttttact ttaggaaatc cagtaatata catcgctgta tcaagcatgt gaatgccata
    19321 atcgatcaaa ggcccgcctc cttgaagttc tttgtcgata aacgttcccc agcctggaac
    19381 accagaccga cgaagcgcat tcgcttctac gtaataaata tccccgagaa catcaagatg
    19441 attcttgatc attgttgctt cgtcagaaaa acgatgctgg aatccataag ccaaaatctg
    19501 cccgttttta tcggctgtct ctgccatctt tcttgcttga tcagctgtca tagctggtgg
    19561 tttctcgcac atcactgaag cattagcttc gagggcagcc aatacatgtt cgaagtgaaa
    19621 gcgattgggt gtacagacac tcacgatatc gggagaaatc tcattcagca tttgtgtaac
    19681 tgatgtatac gcatgagaaa atcctgtacg tcggcaaaag tcttctgctc gctggctgac
    19741 tggatcaacc acagcggcca attctaagac atctgatctt ttttgataat aagcagcatg
    19801 tactttttct gctacttgtc cagcgcctat gattgctact ttcttcattt atttctctct
    19861 cctttgctta tttacttatt gttattcttt ctctattctt tctctaaatc atttaaatga
    19921 cgcgctgttt ccagacagtt tttcaagtat tgcaatgatt cctgataaat tttcacttca
    19981 ttttctcctc gcaaacgaca ttcatatacc atataacctt catactcatt ttgaagtagc
    20041 gtttgaaaat ggcgagtaaa atcaattgcg ccagatcctg gttggtagcg atgattatct
    20101 gctaaatgga tatgacccac atactctttt tgcgtagtca atgcttttga aatatcatct
    20161 tcttcaatat tcatatgata aaaatcagcg atgatctgca catgtttcaa ttgattagag
    20221 tcaatgtaat gacgcgcatc cgctaacaaa ttgatcatat ggtcttggta tcggttcaaa
    20281 ggctccaaat aaataaccgt acctgtttct tcggctactt tatctaaata acgaagtgat
    20341 tcgctgactg ctttatagtc tccttctttg cttcggggtg aaaccatcgg cggtaatcga
    20401 taagtgaaca ttccccaagc tgctgggata acgattcctt tgcctccaac ctcgcttagt
    20461 gcacgcaaaa tctcagcaat ttgacgtaca ccattaagac gccgttcttc gataaagtca
    20521 ccgatccacc catcgtagcc gttacaagct gtcactactg ggatccctgt ttctttgatt
    20581 gcctgtttga ctgtgtcgat attttctatt aacaacttac cgtcgatttc gtattcttca
    20641 aatccacagt ctttgataaa tctaaatttt tctaaaatgt ctcttgggaa aaatgcttga
    20701 tcttgcgtac cgaatttcat tgttctcctc ctcggacttg aacgcccatt ttgatacttt
    20761 cttctgggtg ctggtctaca taacgcatgt aagcttctgc gctttctgca aatggcacaa
    20821 ctggatcaat gatttcttca caatccaagt aaccgttcat caataattcc caacatgtgt
    20881 cctcgattcg tttacggttc catcttgggt aatctgggtt tggttcacta gatgctcgag
    20941 caaagacgat ttttgcatta ttgaaatgtg cttcgcgacc aagattaaac ccagctggaa
    21001 aaggtttagc aaaggcaaca taagaaatga tccgccatag ccgatcccta ttaaagaagc
    21061 ttgtaatgcg gcagcactgc cactcgtttc aataactgaa tctactccac gtttatctgt
    21121 caattccttt agacgaaaac cgacatctgc tttcatagga tctagcgtgt aatcggcacc
    21181 gtgtttttct gctaattctc tacgctgcgc tatgggatcg atcccaacga tggtagtagc
    21241 acctgctttt ttagctaatt gaatcgcaat ttgcccgata gcgcctaaac cgaagattgc
    21301 tacagcatca cctgctcgaa catgtgcttc ccggatacca cctaaagcga attgagcagg
    21361 atcgtaacat actgcatttt tccattgatc caaccgatca agtttacgta gtttataatt
    21421 atcaacaccg tttgtaatca gcgtttcacg aatgggtcca taagtgcaga ctaactctcc
    21481 tttttgatac tctgtcacca aactgcccgt ttcttcgatc gtaccgacaa tcatatttcc
    21541 taattgaaat tcgccaaata ccacaccgcg ttggcttcct tcttctcttg gcaagaatag
    21601 gcgccattct tctgaaaatt cttcatcgat aaatggactt aatcctctaa agtcgacaat
    21661 ttctgttcct ggttttggag tagcgaaaat tactcggata cgtacttcgt tcttttcgat
    21721 ctcacggtct tcgtaatctt tgaatccggc tattcttggt tctaatgcaa ctaatttttt
    21781 catcttttct ttcctctttc ttttcttttt atccttttac gccgccttcg actaaatcac
    21841 ctttaataaa tcgttcagat aacgcataca taatgatgac tggtaaagcg gtcaccaatg
    21901 aagcggccat cattcgtccc caaatgtagt ctggtgtact gaataatgtg ttcaaaccga
    21961 ttggtaaagt aaatttttcg gaggtcgaca ggaaaatcga agcaaataaa taatcattcc
    22021 acgaaaccat aaagcaataa acgaagacag aaatgattcc agaaatagcc aatggtataa
    22081 tgatccgaat aatgatctgg atccggctaa gaccatcgat cattgctgct tcttcaatat
    22141 cagtcggaat cgtgtcaaaa taacttttta gcatgaatat cgcagtagga agtgtttgaa
    22201 cgaccatcgt gataatcagt gctgtattgg tatcatacaa acctagaccg gaaatgattt
    22261 taaataatgg aacaacgatc aaaataccgg aaaacatata aacggtgtaa aagctagcat
    22321 tgattttcga gcgagctttg aagcgtagtt ttgacaaagc gtatgccccg ctgatcccaa
    22381 tcactactgc agttacggag gctatcaagg agatcactaa actatttttg aaatacatga
    22441 tgaatggaaa aatctcagca ttgaaaatat cctgataatg ttcaaaagtc cattctttcg
    22501 gaaaaatagt ttgtgtcgtt gcaattgctt cttgtgaatc cttgaatgat gtcatcagca
    22561 tgatcaaaaa tggaaacaaa ttgacagcta atagcaacaa aatcgtcgcg taaaataaga
    22621 ttttcctgct ctttttattt ttcatttcaa gtccaccttc tttcgtgaga gcaagacgac
    22681 cgctgcaata atgatgaaca atagaatcga tatagctgct gcttttccaa gatcattgaa
    22741 cgcaaaggct gtctgataca aataaacgcc taagatattg actttattcg tcagtagata
    22801 gacatctgta aacatataga acatccagat agatcgtaaa acaatcacag ttgctagtac
    22861 tggtttgatt gccggcagtg tcacgatctt gaacttctgc caagcatttg caccgtccat
    22921 ttcagctgct tcataaagcg ttttgtcgac tgtttgcaaa atagccaaaa atgaaataaa
    22981 cgcatacgga tagtatctcc agatagcaaa caaaacgacc ataaagaagc tagcaactgg
    23041 ctggtcgaac cacagcggtg cttcgttgaa aagctgcaac ctatctacta acagatcatt
    23101 gacgattcca taactattat tgaacatata tttccaagca aaaatcaaag aaatagatgg
    23161 cgtaacataa cttaataaaa tcagtgaacg caccgttttc ctaaatctga acttgcggtt
    23221 aagcaaaata gctacggcta atcctaaagc tgtactgcct gctacaacta agaatgtgta
    23281 agcaactgtt aaacctaaag aacgataaaa ttcactatca cttaaaattt caagataatt
    23341 gcttaatccg ataaaaacgg cgtctgagtt aggattcagc ggcatatcga aaaaactgat
    23401 ctgaatattg gaaagcattg gaatcagcac taaaacagct aataaaagca tgcttggtgc
    23461 taaaagtccc atggctaatt ttaagtcaga atgtttcctt ggacgattca ttgaaacctc
    23521 cccctgataa tctgccaata gctatctact tttttcgaac accattggca gaacttcttg
    23581 ttatttcgta tttttttgtg cgtcagctaa agcttgatcg acatcttcgt ttccgacagt
    23641 tacttggttc actgcttttc cgatcacacc tgagcttgtg atatctccca ttttcgtgaa
    23701 attcttttcc ccgactagtc caaagacttg tacttcatca aaagcactag caatctcaga
    23761 tggcagctcg ccaaaagact tgatgacttc attttctttg taaccatcca gttcgactac
    23821 ttgtttattc actggttgag caccaccagg agacatcaag acccactttt ccatgttttt
    23881 cggttgagat aagaaagctg tgaatgcttc agcagcctct ttttgtttct cgtctagacc
    23941 agaagaaatc gtcaatccag atactgtgcc atagacagct tgatcttttt gtgttgggat
    24001 agcaaaaccg atattttctg agttccccgc ttcataaaca gaaggcaaaa tgtaagttga
    24061 atagattgcc attggtgctg ttccattcat gaaagcatct ttgatttctg tcacatcatt
    24121 agagcctggc atcgtgtatt gggataagtt ttgataaaac gacaatgctt ctctcatctt
    24181 ttccgtatcg atcgttacct cgccttcatc atccaaaaca tttgctccat ttgacaaagc
    24241 aaattgagag aacgcttgct cactcattgt tccttcagca gtaggcatag caattccata
    24301 ttgtttattc tctttatctg tgaaatgttg agcaattttt aaaatatcat cccaattttc
    24361 tggttctgaa aaaccagctt ctgataattt ttctttgtca taccaaatac cttgaaccca
    24421 gccgctaatt ggtgcagcaa tatagctttt tccatcttct gaacgaacaa ggtttttggc
    24481 tccgtcataa taattatctt ctccagcttc ctcgataacg ttttggactg ccgtctgatc
    24541 aatcagttca tctttatcca tcactttagc aaaatcctga ctgacttcca ttacttctgg
    24601 taatttaccg gaacgagcta acgtaacgat ttttgtattg aacgctgatt cttctaccgg
    24661 cacttgcttg attttgatgt ctggattttc tttttcaaaa tcagcaacca gttcattgat
    24721 cacacttaat cgttcctgtt cgacagaaga gtgcatgaat tgaatcgttg tctgttgtga
    24781 ttcgttagag ctttgacttt caccacaagc agtaagaaga aatccagtag ctagcagaca
    24841 ggtagtaaca ataatttttt tcattttttt ccacctctta ttttattatt ttcagccaca
    24901 tatattcata cggctggaat atcaactttc gttcttgact gcaatggata atttgtccag
    24961 aaaacaggtc taggtaatct ccttttggta aaagataacc tgctgattga ttggaaaaat
    25021 tatggataac taagactttc tcttccttcg cttcgcgaat aaaactgata aaagaatctg
    25081 ctgagtccag taagtaaatc aaactattcg gatggaaggc tttttctttt tttcgaatag
    25141 atagcatctg ggtcaattca ccatatactg ctgttcttaa tggatctgtc tgcaactctt
    25201 tctcaatatc tgctaacaaa aatttttgcc gattgatttt tcgattctct cctgtttttt
    25261 ctacgccttt gtaatcattt cgacttccca gcacactttg aatataaatt gccggcacgc
    25321 ctggtaacgt taccaaaaat gaatgagcag ctaaaaagcg tttgcttctc cattccgtcg
    25381 tatctgttgg agaacttaac gcatctagat acgtagcgtt tatctcatat ggactggtcg
    25441 ttccatctgg gtttttcttg tagtttactc gggcaccctc tttcttcagc tgttccacta
    25501 attcaaggat cgttttttcc ggcagaatac ctcgtaccgg attcaatccg ataccgtcat
    25561 gggaggctaa aaaattaaaa aaagttgttt ttttcgatgc gaaatcgatg gtttttaacc
    25621 attctttcaa cttacttgta ttacccgaac gaatcgcttg aagggtcaaa ggtggtagcg
    25681 aaaactgata gaccatctgt gcttcattct cgccattccc aaaataactg atattatctt
    25741 tgtgtggcac atttgtctca gtgatcaata ccgttcctgg agctgcttca tcagcgattg
    25801 cacggaataa ttggatgatt ttatgcgttt ctggcaggtg tatacaagaa gttcctagtt
    25861 ctttccataa aaatcccaca gcgtccaagc gaataaatga agcgcctttt tctaagtaaa
    25921 acaaaagaat atcgatcatt ttcaataaaa cttctggatt tttaaaattc aagtctactt
    25981 gatcttcgct aaaagtcgtc catacgtgtt tttctttccc gtttttgtca atgaacgttg
    26041 taagtaacgg gctggtcctt ggtctagtca cgctgcttgt atctgtatcc agtggcaaat
    26101 caataaaaaa atctttgtac gtatcattca agtttaaata ttcctgaaac caagtacttt
    26161 cggcagacat atgattgcag acaaagtcga acattaaccg tgtttcgctt ctcatttctt
    26221 ctatatcttc ccatgtgccc aaacggtcgt tgattgcctg ataatttaca acggagaaac
    26281 catcatcaga tgtatatggg aaaaatggca agaaatgtac aactctaaat gtatctgcca
    26341 aatacttttg ataaaatttc ttaaatgtac gcaaagttgg ttgctgctct tcaaaaaatt
    26401 gatccccata agtaatcaag aaaatatctt cttcgtccca atgcagtttg cctgcctggc
    26461 ctttcttctg ctgataaaca ctgacttttt gttcaatcat agaggcggtt ttctcagaca
    26521 aatctgagtc gcgataaatc gcttgtaaaa gttttgtata catcggattc ccctttcttt
    26581 tttgggagcg ttcccaacga tttgatcata ttatggaaaa cgcttactgt caatagcatt
    26641 tttttaaata attttgatat acacaaaaat acaaataaga aagcttatgt atcaataatt
    26701 aatagcttct taaaaaaata tggtctctca tgatcatgca agctattcct aaattttcaa
    26761 aatcatagac aaaaaagaat gtctattgta ctatggaatt attctaaaac gctcccaaaa
    26821 aggaggtgca tataaaattg aaacctacta tttatgacat tgcgaaaaaa gctaatgtct
    26881 caaaatcaac tgtctccaga gtattaaata accagccaaa tatctctgat aaagcaaaag
    26941 caagtgtact tcaagcaatc aaagaattga attatgaacc gagcaaaatt gcaagaggac
    27001 tgtcaggaaa tggttttgac gcgattctag cattatctca tcgtacttct catacgacct
    27061 atggcaatcc ttttttttca gatgttattc aatccatttc tgccgtttct gaacaaaata
    27121 acttcgactt gattctacaa acggcaaaaa caccagaaga agagctagaa aaatgcgcga
    27181 aaaagatcca agaaaaaatg atccgaggaa ttattatttt aagctctacg atggatgaaa
    27241 cacttctttt tgaattggat caatacaata ttcctattgt ggtaatcgga aaagtcggaa
    27301 acgactacac gaacatctac tcagtggaca cggataattt ccaggatagt tacgatttgg
    27361 tggaaacact gatcaagaat ggacaccgtc atatcggctg tctccatgct ccgacagaag
    27421 tttatgtatc caatgatcgt ctcaatggtt accgtcaatg cttatttgat caccacctag
    27481 atatccgtaa tgaatggatt atagacggcg gctttcgctt agaagatagc atggcagcaa
    27541 tgaatgattt aatgaagcta cgagaaagac caactgcgat tttttctacc gatgcattaa
    27601 aatcattgag tatttacaaa gtggcagaac aatctggaaa gcagatacct gatgatcttt
    27661 cgatcgcagg attcagtgat cccttattgt cctccctatt ttcgccacag ttgactcgga
    27721 tagacattcc gacaaatcag ttaggaatca aagccacaga acttttattc caactaatcc
    27781 accaccaata ccctgaagaa aaaagaatca ttctttcaac cgagattact ctcacagatt
    27841 cgatccaaca acaagaaatg tagaacagaa ttataaaaga acagaaacac tgaggataag
    27901 tcagttactc tgttcttttt tcaatcactc atccctcgtt accccctccc ggtattttag
    27961 cgaatttttc ttattttcca tctgttttag atagctatgg ataactataa tgtttgtgtt
    28021 aaactatttt ttccacaggg aagaaaaaac tatttttaac cacgttgtgt ataagttttt
    28081 atagttcgtt tttttaaaca catttaccct tatttcaatc catgttattt ggggatactt
    28141 atctctaaaa cataaataga ataacagaca tgtctactgg ttttgtttta ttcgaggaat
    28201 tattcaataa tagctagcaa aatattaaaa ataaccgctc tatcttccca tcaatttatt
    28261 ttgcattcaa aaaatgatct atctttactt ttgttgattg acttctcttg gaagatgatc
    28321 gttccgatac cttaattgga tacgaaccga tgttgttaag atcctttccc acttttcttc
    28381 tagttagaca aattatttta gacaaaccaa aaaagaggtt gtgacaataa tttgtcacaa
    28441 cctctttttt aaaatatcta ttttgtttga ttgttcattg cgtcttctgc agccatttgc
    28501 gcttcaatat ctgaaatact gtagacattt tcgcttgcta caccgacttc ttttggttcc
    28561 atgtcacgat aacgttgcat accagtacca gctgggatga tcttacctat aataacattt
    28621 tctttcaaac caagaagagg atctttctta ccgcggatcg cagcatccgt caagacacga
    28681 gttgtttctt ggaatgatgc agcagataag aaactatttg tttctaatga tgcttttgtg
    28741 atacctagta agacaggtcg agaagttgca ggaacgcctc ctgagactag cgtattgtag
    28801 ttttgatctt taaagtctgc gatgtctaat aatgtacctg gtaagatatc tgtatcacct
    28861 ggatccatta cacgaacttt acgcagcatt tgacgaacca ttacttcgat atgcttgtcg
    28921 ccgatttcta ccccttgcat acggtaaaca cgttgtactt cacgaagcaa gtagttttca
    28981 acagataata catcgcgaac ttgcaacaat tgttttggat cgattgatcc ttctgtcaat
    29041 ggtgcaccac ggtggatcat atcgccttct gctactttca tacgtgctgt ataaggaaca
    29101 gtatacgtac gtgtatctgt ttttcctttg attgttactt ctttggtacg tgttgcagga
    29161 tcttctgaga tatcgatgac atcccctgtt acttctgtga tcactgcttg ccctttcgga
    29221 ttacgtgctt caaagatttc ttggacacga ggaagacctt gagtgatatc gtctccggca
    29281 accccacctg tatggaatgt acgcatcgtc aactgagtac caggttcacc gattgattga
    29341 gcggcgatcg taccaactgc ttctccgact tcaacgtcag agccagttgc aaggttgcgt
    29401 ccgtaacaat gtttgcagac accatgtttt gtattacatg tgaagacaga acggattgtc
    29461 actgtttcga cacctgcatc aacgatttgt tttgcgatgt cttcggaaat caattggttt
    29521 cctgcaatga tcaattcatt tgtttcagga tgacgtactt ctttacgggt gtaacgtccc
    29581 actaaacgat cttctaatga ttcgatgatt tcatttccct cgcggatggc ttggatttca
    29641 agacctcggt cagtaccgca atcttcttca cggatgatta catcttgggc cacgtcaacc
    29701 agacgacgag tcaagtaacc tgaatcggca gtcttcaagg ctgtatcggt catcccttta
    29761 cgagccccgt gagttgagat aaacatttcc aagacggata gtccttcacg gaagttcgaa
    29821 atgattggca attccatgat tcgaccatta ggagcggcca tcaaaccacg cattccggca
    29881 agctgtgtaa agttggaaat gttaccacga gctccagaat cagacatcat gaagattgga
    29941 tttttcgctt ccaaaccttc catcagtttt tgttggatgc tgtcttttgc agcattccaa
    30001 acagcaatga cacgttcata acgttcgtca tctgtgatca gaccacgacg gaattgtttt
    30061 gtaatagttt caacttgttt atgggcattt tcaatgattt cttgtttttc attcagtaca
    30121 ctgatgtcag cgatccctac tgtgataccg gcatgtgtcg aatgtttgta ccctaagtct
    30181 ttcatacggt caagcatctt agaagtttct gttactttga atcgtttgaa gacttctgcg
    30241 atgatgtttc ctaagttttt cttcttgaac ggtaatacta attcttgctc tttgatatgt
    30301 gcaggaatat cagtaccagc ttcaacaaag tatttgtccg gtgtttggac ggtcaagttg
    30361 aagtctgttg gttcattcag ataagggaat tcaggaggca tgatctcgtt gaagatgatc
    30421 ttaccaactg ttgtgatcat cgtacgttct ttttgccatt cagtaaatgg tttttcacct
    30481 aatgagttag ggttcacacc gatacgtgaa tgtaaatgaa catatccgtt gcgccatgcg
    30541 ataacggctt cgttttgatc tctgaagacc attccttcgc cttcgcgtcc ttcttcttcc
    30601 attgtcaagt agtagttacc tagaaccata tcttgagaag gagtaacaac tggtttacca
    30661 tctttagggt tcaagatgtt ttgagcagct aacatcaaca tacgtgcttc tgcttgtgct
    30721 tcttcattca atggaacgtg gacagccatt tggtctccat cgaaatcggc attataagct
    30781 tcacatacaa gtggatgcaa acggattgcg cgtccttgta ctaaaactgg ttcgaatgct
    30841 tggataccaa gtcggtgaag cgtaggtgcc cggttcagca atactgggtg ttccttgatt
    30901 acgtcttcta aaacatccca tacttcgtct tcttgacgtt cgattttacg tttcgcattt
    30961 ttgatgtttg aagctagttc gcgttgaaca agctcacgca tcacgaatgg tttgaataat
    31021 tcaatggcca tttcttttgg aagtccacat tggtacatct tcaagaaagg tccaacaacg
    31081 ataacggaac gacctgagta gtcgacacgt ttacctagca agttttgacg gaaacgacct
    31141 tgtttccctt tcaacatatg agaaagagat ttcaatgggc ggttacctgg tccagtaact
    31201 ggacgtccgc gacgaccgtt atcgatcaat gcatctactg cttcttgcag catacgtttt
    31261 tcattttgaa cgatgattcc aggtgcattc aagtctaaca aacgtttcaa acggttatta
    31321 cggttgatta cacgacggta aagatcattc aagtcacttg ttgcaaaacg tccaccttct
    31381 aattgaacca ttgggcgtaa atctggtgga ataacaggga taacgtccat aaccatccag
    31441 cttggttgat ttcctgaagc gcggaatgct tctaaaatat ccaaacggcg gattgcacgt
    31501 gtacgttttt gtccttgtgc agatttcaat tcttctttca attcggctgc ttctgcatct
    31561 agatcaacat tatcaagtaa ttgtttaatc gcttctgcac ccatagcagc ttggaattct
    31621 tgtccatatt gttcacgttt ttcgcgatat tcgcgttctg tcaacaattg ttttttctct
    31681 aaattcgtat ttcctggttc gatgactaca tatgaagcaa aataaatgat ttcttccaaa
    31741 gcgcgtgggc tcatgtctaa tacaagaccc atacgtgaag gaatcccttt aaagtaccag
    31801 atatgtgaaa caggagctgc taattcaata tgtcccatgc gttcacggcg aactttcgaa
    31861 cgagtaactt ccactccgca tcgatcacaa acgattcctt tataacggat acgtttgtat
    31921 tttccgcagg cacattccca gtcttttgta ggaccaaaaa tccgctcaca gaacaaccct
    31981 tcgcgttcag gttttaaggt acgatagtta atcgtctcag gtttttttac ttcaccgtaa
    32041 gaccagcttc tgattttttc gggagaagcc aaacctattt gcatactttc gaatttattt
    32101 acatcgatca aaaggggttc cctccatttc atttaatggc aaaccgcaag acggctgatc
    32161 ttcgaccgcc ttgcagctca tagaccggat tagtctaaat tatcttctgc ggtttcaaag
    32221 ttttggatga catcgtcttt ttcatcttcc acttgttcag cagctttctt ttctaattct
    32281 ttggcagttt gctgttcggc aaattttgtc aaagcatcaa cagtgatcaa atcgtcgtct
    32341 tcatcgtcca tgtcgcgaag ttcgatctct gtttcttcga tatccaagac acgcatgtcc
    32401 agacctagtg attgcaattc tttcaccaat acgcggaatg attccggtac acctggtttt
    32461 ggaattggtt cacccttgac gattgcttcg tatgttttca cacgtccgac tacgtcatct
    32521 gatttgtatg tcaagatttc ttgtaaagta taagccgcac cgtaagcttc cagtgcccat
    32581 acttccattt caccaaaacg ttgtccacca aattgagctt tacctcccaa tggttgttgt
    32641 gtaaccaatg agtaaggtcc gattgaacgt gcatgcaatt tgtcatcgac catgtgagca
    32701 agtttgatca tgtacatcac accgacagac acacgtccat caaatggttc acctgtacgt
    32761 ccgtcgtaca gaacagtttt cgcatcgctg gccataccgg cttcacgaac tgtttcccat
    32821 acgtcttcat cacttgcacc atcaaatact ggcgttgcta cgtggatacc taactgacgt
    32881 gcagccatac ccaagtgtaa ttccaatact tgcccgatgt tcatacgtga aggcacccct
    32941 aaaggattca gcatgatatc gattggtgtt ccatcaggta ggaatggcat atcttcttcc
    33001 ggcataatac gggatacaac ccctttattt ccgtgacgac cggccatttt atccccttca
    33061 tggattttac gtttttgaac gatataaaca cgaactaaca tgtttacgcc tggtgacaat
    33121 tcatctccag cttcacgagt aaagattttc acatcgtgaa cgataccgcc gccgccatgt
    33181 ggtacacgta atgatgtatc gcggacttca cgagcttttt cgccaaagat cgcatgcaat
    33241 agacgttctt ctgcagataa ttctgttacc ccttttggcg tgactttccc tacaagcaaa
    33301 tcgccatctt ggacttctgc accaatacgg atgataccca tttcgtctaa atcttttaat
    33361 gcgtcttccc cgacattagg gatttcgcgt gtgatttctt caggtcctaa ttttgtatcg
    33421 cgggcttctg attcatattc ttcaatatgg atagaagtgt atacgtcatc ttttaccaaa
    33481 cgacggctca tgatgatcgc atcttcatag ttgtaccctt cccaagtcat gaaaccaact
    33541 aaaacgtttt ggcctagagc catttcgcct tgttccatag atggtccgtc tgccagagta
    33601 tccccttttt cgactttctc acccaaatga acgattggac gttggttgta gcttgttccg
    33661 gagtttgaac gacggaattt tgttacatcg tatttatcta atgcaccgtt gtcgcggcgt
    33721 acgcggattt cagaagcatc aacgtattca acgacaccgt catgtttaca taacaaagca
    33781 gctcctgagt catgcgctga tttgtattcc ataccagtac caacccatgg tgcttgagga
    33841 ttgatcaacg gcactgcttg acgctgcatg ttggcaccca tcaaggcacg gttggagtca
    33901 tcgttttcca agaaaggaat acatgctgtc gcaacggcaa ctacttgttt aggtgatacg
    33961 tccatgtagt ctactttatc aatagatact tctaagtttt cactttgagc acgagccata
    34021 acaacgtctt gagcaaatgt tccatcttca ttcaatgggc tgtttgcttg ggctacgatg
    34081 taatggtctt cgatatcagc tgtcaagtag tcgatttggt cagtaacacg acctgtttca
    34141 cggtctacac gacgataagg tgtttcgata aatccgaatt tatttacttt cgcataactg
    34201 gacaaactat tgatcaaccc gatgtttggg ccttcaggag tttcgatcgg acacatacgg
    34261 ccatagtggg agtagtgaac gtcacgaact tcataaccgg cgcggtcacg agtcaatccg
    34321 ccaggtccta aggctgaaag acgacgtttg tgtgtcaatt cgccaagagg gtttgtttga
    34381 tccatgaact gtgacaactg agaagaacca aagaattctt tgatacttgc cacaactgga
    34441 cggatattga tcaattgttg aggtgtcaat gtttcagtat cttggatcga cattctttca
    34501 cgaaccacac gttccatacg tgctagcccg atacggaatt ggttttgtaa aagttcaccg
    34561 actgaacgga tacgacgatt tcctaagtgg tcgatatcat cgacattgcc gataccttcc
    34621 atcaagttca agaaatagct cattgaagca atgatgtcgg ctggacgaac cgtttttaca
    34681 gctgcttctg gatagccgtt tccgatcacg tttacttcac gttcagggtc tcttggtgaa
    34741 aatactttga tgacttggac agtcattgga tctgtgacca caccgtcttc tgatggatag
    34801 taagtcactg aatttaggcc attatcaatg aaaggagcta gtgtttccat gacttggtga
    34861 gtcagaactg ttcctttttc aacgatgatc tcgcctgttt ctggatcaac taatgtttca
    34921 gctaatgtca gattcaatag acgtgttttc aaatctaatt ttttgtttac tttgtaacga
    34981 ccaacgtttg ctaagtcgta acgttttgga tcgaagaaac gagcattcag caagttacgt
    35041 gagctgtctg ctgtttttgg ttcgcctgga cgcaaacgtt cgtaaacgtc tttcaaacct
    35101 tcttctgttc ttgagtcgct tgcgttttta tgcaagtctt tttcgactgt attgcgtaat
    35161 gtttcgctgt cgccgaagat ttcaaagatc gtatcatcgg aaccaaatcc taacgcacga
    35221 actaatacgg ttaaaggaat cttacgtgta cggtcgatac gtacataaga gatgtctttt
    35281 gcatcagttt ccatttctag ccaagctcca cggttaggga taacagtaga accgaatcct
    35341 tcttttccgt ttttgtcgac ttttccatgg aaatagacac ctggtgaacg gactaactga
    35401 gaaacgatta ctcgctctgc cccgttgatg atgaatgttc cttgttctgt cattaatggg
    35461 aaatcaccga agaatacttc ttgtgctttg atttcgccag tctcgcggtt tgtcaaacgc
    35521 aatgttacat gtaatggtgc agagtagttt gcatcatgcg cacgagcttc tgctactgta
    35581 tactttggtt ctttcaattc ataatctaca aattccaaag atagattgcc attgaagtca
    35641 tcgattggca aaatgtcttc aaacatttct ctaagtccct catctaagaa ccattggtaa
    35701 gagtctgttt gaatttcgat caaatttggt aattccaaca cttcactgat tcttgcgaaa
    35761 cttctacgtt cgcgatgttt tccgtatttt actacgtgtc cagccaagct cttcacccct
    35821 ctaaaatgtc attcaaaaag actacccgat caattgttac aataatattt caaatattat
    35881 ttttaggtaa caacagacat tttttgcggc ttttaggcaa aaaaaaacca aaaatagaac
    35941 attctcttga aaccttactt cgtgagcttc tacttttgtt tccttatatt aaagccgaaa
    36001 cggacaatat ttcgtttctg ccttttcttc atcagatagt ttttgcaact tcatatacta
    36061 cacttatttt ctataaaagt caacacttcc tggcgtttct tctgcttttt cgcaacattt
    36121 tcttaatgtt ttcaatttta tgttttcaat tatctgacaa ttcttaattg acagaccttt
    36181 ttatctcggt tatactttag aaaattcatt ttgtggagga acttctatgc gaaccaacat
    36241 caaccaaatc gcaccaacac aactgaataa cttttacttt agctacttta gataggtttg
    36301 tattcatcat gatggataca aacgttaaac gacagtttgt atctatgatg gctaaagaac
    36361 tttgtattgc agcaaaaagc cacgtagaca aaatgctaag tggctgagca gtgaaaatac
    36421 aaagtaaaag tatcaagcat tctacttttt caatttattt tccgactttt caatccattt
    36481 ggacattttg tctgaatgga ttttttgttt cccaaaatta aggaggaaaa agaaatgaaa
    36541 aaaaatataa tcatcggatt aggcgctgcc ctgctactag ctttaggagg ttgcgcacaa
    36601 aaagacaatt ccgcagaggc atcagatgga caaaaaacaa ttggcgtatt gcaacttgta
    36661 caacataact cgttagatgc tgcctataaa ggattcaaag aaggattagc agaagaaggg
    36721 tataaagaag gcgataatct aacaatcgat tatcaaaatg cccaaaacaa tcaagacaac
    36781 ctgaaaagta tgagtgagaa actcgtcaaa gaagatcctg atttgctact tggaatcgcc
    36841 actccagcag cacaatcttt attaaatgaa acaaccacta tcccgatgac tgtcacagcg
    36901 gtcacagact tggaacaagc ccatttagtc acatccaatg aaaaacctgg cggcaatgtg
    36961 actggcacga cagatatggt gccaatcgaa aaacaaatcg gcttactgtt aaatatcgtg
    37021 ccagacgcca aaacaatcgg catcatgtat aacaacggtg aagcaaactc taagatccaa
    37081 gctgatttgg cagaaaaaga attgaaaaaa gcgggcgtca aagtcaaagt actgacagca
    37141 aattccacaa atgatgtcca acaagtgacg aaaagtctag ctaaagatgt agatggaatc
    37201 tatattccta ctgataacac gttcgcaagt gcggcaagtg ttgtcggtga agtcgcaaaa
    37261 gaaacaaaaa cacctgtcgt tgctggcgca gttgatcaag tcaaagacgg cggtctagca
    37321 acagtaggaa tcgactacga aagcttgggc aaacaaacag ggatcatggc agcaaaaatc
    37381 ttagacggca agaatccaag tgaacttcct gtcgaaacag cgaatgattt ggaactgtat
    37441 gtcaataaag acatggccaa agcattaggc atcaatccag acagcatcaa agaagctgaa
    37501 taaaacacag gaggattctc atgacaaatt tgctctctct tttcttatca tctgcatcgc
    37561 aaggggtctt atgggcccta cttgcgattg gtgtatacgt cactttccgt atcttggaca
    37621 ttgccgactt gactgctgaa ggaagctttc ctttaggtgc agggatcgcg gcagtaagta
    37681 ttacaaatgg ctatcatcca ttgacagctt gtcttctagg ctttttaggg ggtgcggcag
    37741 cagggcttgt ttccggactg cttcacacaa aattgaaaat tcccgccctt cttgcaggga
    37801 tcatcaccat gactggttta tattctgtca catcccgtgt gatgggtgcg ccgaacatct
    37861 ctttattggg tcagccaacg atctttcatt gggtcgagtc attcggcata acaaaagaca
    37921 acgctgtact gatcgtcggt ctgctttttg ctttgatcgt tgtatgttta cttgttttgt
    37981 tcttacgtac agaaacagga cttgctattc gtgcaactgg cgataatctg gcaatgagcg
    38041 aagcaaacgg gatcaacaca gataatatga agatcatcgg ttatatgatt tctaatggat
    38101 gcattgctct ttctggctcc ttacttgcgc aaaacaacgg ctttgctgat ttaaattctg
    38161 gtattggaac gatcgtaatt ggacttgctt ctattatcat cgcagaagtt ttgttacgta
    38221 accagccact atttctccgt ctcttgactg tcgttttagg tgcggtcatt tatcgtttta
    38281 ttttagcgtt agtcttcgaa ctgaatgttg aaccaagtga ttcgaaactt gcttctgctt
    38341 tcgtgctagt gatctgtctt tctttcccgc agatccagca aaaattccat ttacctcaaa
    38401 aaaagaaagg cggtagtgtc aaatgaatga cgtcttaaca ttggaaaatc tccatcatac
    38461 ctttgaaaaa ggaacgatca acgaaaatca tgtcctgaaa ggcattaatc tttccttaga
    38521 tcccggcgac ttcgtgacga tcatcggtgg aaatggggct ggtaaatcta ctttgctgaa
    38581 cagcatcgcc ggtacatttt ctgtcgatca gggtcaaatt ttattgaatg gaaaaaatat
    38641 cacgaaaaaa tctgttgtcg aacgttcgaa atccatcagt cgcgtattcc aagatcctaa
    38701 actagggaca gccgttcgtt taacggtaga agaaaatctt gctcttgcta tgaaacgagg
    38761 aaaaaaacgc ggcttcttcc gtggtgtcaa accacaagac cgaacatttt tcaaagacca
    38821 gctggttcgg ctaaatttgg ggttggaaaa tcgtttatcc tcagaaatcg gtttgctgtc
    38881 tggaggacaa cgtcaagcta ttacgttatt gatggccaca ttacagcgtc cagaactgat
    38941 cttattagat gaacacactg cagcgcttga ccctgctact tccaaaacgg tgatggaatt
    39001 gacagaaaag ctgatcactg agcagaatct cactgctttt atggtcactc atgacatgga
    39061 agacgcgatc cgctatggga gtcgactgat catgctgcat caaggaagga tcgtcgtaga
    39121 tatccagcaa gaggaaaaac agcagctcac agtgccccaa ttattagcgt tgttcaaaca
    39181 aaacagcggc acggcattaa aagacgatca agtactttta tcttcataat cagaaagagg
    39241 atgggacaaa aaatgaacgc ccaccctctt cttttttctc tttccttctt tcgtttttgc
    39301 tttatgtatc aaccgttgtt tcttcgaaaa ctattttgtt ctctccttct tcctgataga
    39361 atggatggga aaggagcttg atgccatgac aacgaaagac aaggtacttc aattattgaa
    39421 aaagacacca gattttcttt ccggagaaaa gttggcacag caactggaag tttcccggac
    39481 aagtatttgg aaagccatca aagaattgga aaaggcaggc tatcgttttg agcacgggcc
    39541 tacagggtat cgttacctgc cctctgacgt actagatgca aaagaaatcc agcaagagct
    39601 acggtcgttt attcctgatt tgaaagtaca aacgatgttg acttctgaat ctacgatgaa
    39661 agatgccaaa ctggcagcag ctcaaggaga ttctgtttcg aagttatttg ttgctgatac
    39721 acaagaacaa gctcgtggac gttttggacg gccttttttc gctgaacctg gacgaggaat
    39781 ctacatgagt ctcttgctcc atccagacaa acggtttgat gaattgcctc aatatacgat
    39841 catcgcagcc agtgcctgtg tgaaagccat tgaatctgta accggtcgtt ctgcttcgat
    39901 caaatgggtc aatgatatct acatggatgg aaaaaaagtc tgtgggattt tatctgaagc
    39961 agtcagtgat atggaaagcg gcaggatcag tcatatcatc attgggatcg ggcttaattt
    40021 ttctgttcct caaaatcatt ttccagctga tctccaagaa aaagccatct ctgtctttcc
    40081 cgacggacaa gctagtgtca caaggaatca gctaatcagc aaaatctggc aagaattgtt
    40141 cacttatctt tctcacccag aaaaagcgtt ggacttctat aaagaaaaat cattcgtatt
    40201 gggaaaaacc gtgtcgttca atcaaaaagg aacgacttat gtcggtcttg caaaagaaat
    40261 cggtgatcat ggcgaactga tcgtccaaac aaatgaacag aagatgagtt tgtcttctgg
    40321 cgaaatcagt ctcacatcta tccgtacgca ataattatca gatttcacaa aaggacacct
    40381 gatctcatac agtgtccttt tttctgaatt ctcttcaaaa gactctcctt attctatcag
    40441 ctgacagttt ctctatcctc tagaaaaagg tattctaaaa atatctataa agcatggtac
    40501 actttcttta aatgaatcaa tgcaaaaaac aagagagtca tcagtttaca cgatcaaaag
    40561 agatagcagg ccgcttcctt tttcaatctt atagatagac catgggtaac gataaaaaca
    40621 aaaaagaact tgcgacttat tttgccgtaa gttctttttt agctagtgcc ttaatctctt
    40681 aatcccaaca aacgtctcgc atagatcacc tgaccagagt gcatagcagc atcatcaata
    40741 atagaaacga gacgcattcc tcgtgtgact ggtggtgtcc agttctcgtc aattacttca
    40801 tctagctcgg aaggttccag ccttcttaag taagaaatag caaaatcagc agctcctcgc
    40861 aagtatccta gcagatcttc tatttgatta gcttggatct gctttgcttg ttccagacta
    40921 tgcttccaat cttgttcgtt atctgctatc gtcatcggaa accggctttc ccagtcttga
    40981 ctatgccaaa ttggagaagt tcctgctaaa tcagcaatct ggaaatctaa ttctcgagcg
    41041 gtatgccaag ccagccaagt cattgatttg atctgcgggg ctctatcttc cactggaaaa
    41101 cgattggcct cctcaataga gacgccttca aacaaacgtt caaaccgatc gtatgcccga
    41161 tccacactct cgcatagcat atctaaataa tccatttatt ttcctccttt ttatttatcc
    41221 tacttacttt ttcctaattc taggatacct acttatatgt tactacacac ccttattttt
    41281 tacactttaa cagtcaccat catcaatagt ttggagattg aaagaaaaag atagtgtcga
    41341 actatcaatt gaacaagaag caacaatatg aacgtttcat taatattctt taaataaaca
    41401 atgatttttc aaacgaacag tttaagacta ggagaaaaag agtataatag gaaaatgttt
    41461 gaaaggaaag gaaggggaaa aatgaaaaaa agggcaagtt tactagtcct agttgctttg
    41521 ttgttcaata tcttttcgta tggtgtggcc gtttcagcgg aaacatacat tcctaaagac
    41581 cctgggaata tttataatga tctaactggt aactatggct tgttaggaat tgctagtcaa
    41641 ttccacgtat ttgctaaaaa taagacaact gtgaatgcac atacagacgg aaatatagca
    41701 acacatgaac ttgatgcaaa aaataatttt ggtacagagc ttcatgaagg tttattgaac
    41761 caagaaataa attatataca aaaaactgat tctttcgcta attcttcagg catacctaca
    41821 ggtagcacaa gaatgaataa atttgtcgtt ggcagtaacg tggatgtagc acttcaagat
    41881 ggtcatccta cagtcaatgg atccaggctt gatcacttaa ctccagatga tttttataaa
    41941 gatcgtgctg ggcatgaata tattgatttc gagcaggaat ttgctaaatt gaatgtagca
    42001 gcaaatgatc ttgcaacgat cactccagct aaaacatata ctgctgcaga ttttccagac
    42061 atgaacaatc ggacaatcga tttaactgat ttatcagata ccgggttttt attggtaaat
    42121 atcgatgcag aagtgttaac gatgaatacg ccacttcaaa tcattaatcc aaatgatcaa
    42181 gtagttgtat ttaatgtcat caacagttct tctgcattga acgtacagtc accaatcaaa
    42241 tacaatgatc gttcaaatca tgaaacagaa gatttttcgg atgcgaatat ctcttggaat
    42301 tttggaaatg agatgactga tttgacaatc agtgctcctt ttcaaggaac aatccttgcg
    42361 cctaatgcta ctatccgcat tactcaaaac caagacggaa cgattatagg aacaaatgtg
    42421 attttagatg cagctaccaa tcgttgggat cctaacgaaa tatttatcag cgacaatggt
    42481 acagatacga cagacacaag cgacagtaca gatacatcta ccacaagtga tagctcagat
    42541 tcatcaactt caagcgacag tacagatact acctctacca caagtgatag cacggataca
    42601 tcagcttcaa gcgacagtac agatactacc tctaccacaa gtgatagcac ggacacatca
    42661 gcttcaagtg acagtacaga tactacctct accacaagtg atagcacgga cacgtcagct
    42721 tcaagcgaca gtacggatac atctaccaca acaagtgata gcacggacac gtcagcttca
    42781 agtgacagta cagatactac ctctaccaca agtgatagct cagattcatc taccactaca
    42841 agtgatagca cggacacgtc agcttcaagt gacagtacag atacatcctc taccacaagt
    42901 gatagctcag attcatcaac ttcaagcgat agtacagaca cgtcgtctac cacaagcgat
    42961 acacgtcatg caagtgtctc cttctcaaaa acaagttcgg ataacaggac gaactttaac
    43021 ttaactaata aaacaagcaa tgacaatgga aacaaatcaa aaagagcact tcctaaaact
    43081 ggaagtcaat cgaataattg gataacactc gcaggcgtta tattacttgt aatcggttta
    43141 agaatcactt ttatgagtta taagaaatcg aaaaggtaaa aaaatattca agataaaaaa
    43201 gagcaacgtc gctaacggac gttgctcttt atttttgctt tttatcataa atacaagaac
    43261 tacaattcaa tttgtcaaaa aacacgtaga tttccttctg agaaatttat ttacgtatcg
    43321 aacctttccc tattcagtct ttgcgatcca aaatcagttg ggtaagggaa aagagattct
    43381 cttttgttcg aagagaggtg gcaggtaatt tctttatttc cttcaaagcc ttttctgtgt
    43441 aagaggccgc taacttccta gcttcttcca cgcctcctgt atgttcaacg attttgcgta
    43501 cggcgtcgac atcttcttct gtcattgccg ctttcttctc taaataaggt agtagctttg
    43561 gtttgttttt ttgcaaagag tagatcaacg gtaaagaata gaccccttga cgcatgtctt
    43621 ctagtacagg tttgccgatt tcctggctct cttttgtata atctaaaata tcatcgataa
    43681 tctgaaaggc catcccgata gaaagtccga tatctcctgc tttttttgcg aagcgctcgc
    43741 ttgttccact ttcaaaagcg ccaacagaac aacttaattg gaacagttca gctgtcttac
    43801 ctgaaatatt ttccaaatat tcttttacag aaagttcata attatagcga tcatccatct
    43861 gccctaattc gccattcaat actttttcca tactgcgaga attcaattgg agacttttca
    43921 aggaagtgga gtaatcagac aatagtttaa aacagcagac aaataaatag tctcctgcat
    43981 agacagaaac gacattacca aactgactac ggatattagg ctgaccacgt cgcagatccg
    44041 cttcatcaac gatatcatca tggatcagtg tggccgtatg gagcatttcg atagaagctg
    44101 ccaaagcgat ggctttgtct cgatcttgct ctggtccaaa ttgtgagaac aatagctgat
    44161 aggcaggacg caacagcttt ccgccggcat ggatcgtttc tttgatggct tgttcgacag
    44221 gtttgttttt caggcggaca gtttcttcca tccgttttag tgtttctgtc aattctttac
    44281 ttaaagcagg atagttttcc cacattggat gtagcttcat aaaaaaactc cttgtgtgac
    44341 gattattttt tggtaaacat tgggctccat ttttgtttgg ttccctgctc ataatctttt
    44401 tggaatcgtt gaagtgtctg cacatgtcgt aaaagataat tcgctgcgat cccgattgca
    44461 atccctgata aaatccctaa aactgaaagg actggcaagt acaacatgac tgtccatgat
    44521 tgtgcaatga aagaagccat caaaagctgt ccgacattat gcatgaaacc gccggccgca
    44581 ctaattccga tgatactgac acgttttggt cctaattgct tgaccaataa cattccaaaa
    44641 taactaagca aagcaccact catactataa aggaatgtgg aaatcgttcc gcctagcaaa
    44701 gtagtcagga atagtcgtag ccaaagtagt aagaagctgt cttgtttttt catggtaaaa
    44761 atggcaacga tcgtgattag attggctaag cctagttttg caccaggcgc aaaagaaaaa
    44821 ggataaggga tcatattttc caataaacca atgatcactc cttgtgcaac aagtagcgaa
    44881 atataaatca ttttttgtaa tttactcatg gtgtgtgaag gtctaataaa tcagactgtc
    44941 ttcgtcacct ccatctgttg aacggacttc gatcactaat ttatgtggca aacagacgat
    45001 ggtttcgcca tttttcgttg cccagcctcg acgtacgcag atctggtcat cgcaatctgc
    45061 ttcttttatc cgtactcgat cgccatctat ctcgatcaag ttataatctc cgtgttcatc
    45121 tttgtaagta tatgtgttgc tctcttctcc agccatcaaa gggaatgttt tgatttctgt
    45181 gccatccaca cgcagtaccg cttctttttc tgctgttgcg gtgttttgct gaagaccaaa
    45241 aatcatgact ggtaaaaaag aaagaacagt caaaagcaaa ataataaaaa catcccaagg
    45301 tttgagcaga cttttttcag caaattcttt aaggctcatt tttgttctcc tttttccaga
    45361 ttaactcacc caattttagc ataaaaacaa gaggttgtga cacctgtgtc ttgctttgag
    45421 taataagaac gaagaataga aaacagcttt ccatgttttc taattctttg ggcttattac
    45481 cgagaagcag caggtggaac accgtttata acaagaggtt gtgacagaag tgggcaactt
    45541 caagaaataa gaaggaattt acgaaaatta cttttcaagt ttttgtgaaa tttcggctta
    45601 tttttcgaag aagctacttc tggaacaccg tttataacaa gaggttgtaa cagaggttgt
    45661 gacacctgtg tcttgctcat ggaggcttat ctcgggcatc ttattccttt aattttacat
    45721 aaaaaggagg ctgtgacggt ttgtctcagc ccctttctat tgaaatcttg cattcaaata
    45781 acgttcttta tctatttatt tcttgccgta ctgggaactt ggtctaccgt accaccaatt
    45841 acctagtgta cgttgtagcc atggaattac ccagcgatcc aacccaatcg cacgtccgga
    45901 accgttcatc aatgcgaaag caacaaaaat gaaccaaatg tttacccagt aaaacatgcc
    45961 tgataagcag aacgtgatca ccaatacaat cgttgtcgca ctgcatagcc atgtgaataa
    46021 tccagcaatc aatgctaatg ccaaggcaat ttcaaagaat accatgaatt tttggaagaa
    46081 cattgctaca tcattgtttg gcatgatcac ttgcatgatc tttgtgaacc agtctggtga
    46141 cttctcaatc acagccattg gttcttcacc gtatgcataa ctcaaaccaa aatgtgcagc
    46201 ttgagtcgct gcttctccgg cacttgcccc tgctccgctg gcagctgtgg ttgcatcacc
    46261 agcagcagaa gcaccactgg caacatctgt tacattgtat tcccatgtcc aagggaacac
    46321 actgtttcca gtgaaccatg atccttcacc aaaccaagta cttggtttga acacttcacc
    46381 attcccaatg atttttttgg aagcttcaac gaaccaaact aaaccataga aaatacgaag
    46441 tggcacactc cataagacat tgccgtaacg agaagaatgt cctcgagtca cgttgcgatc
    46501 atctttgata tggaaaaact catgcattaa atattggaac atataataac cagaacgaat
    46561 atcaaagaag tatttcaaat tcacgatatg tttcattacg atagctaaaa atccgcttaa
    46621 atggatctta tcaaataggt tagctacccc ccatttggca ccaatagaaa ccataaatcc
    46681 ttgatagttt cctttaaatt tatgcttttc tccgcctttg atcgaagcca cgatattcgc
    46741 tgcggctgtg tgtcctgttt gttcagcagc ttgaacgatc tgcggtgtcg gtgttcctgg
    46801 gaattcttca taaaagacta aatcaccaat gatatagatg tttttatctt cgtagccttt
    46861 tgcctgcata tattcattgg cgatcaaccg ctgtccgcgt gctgattcta atccaaagtc
    46921 agcagcgtca gaagttcctt tgacaccagc tgtccaaatc agtgtgtgag tcggaatgtt
    46981 gcgtccgtct ttcaatttga tatgatcagc agccacttct acgattggtg cattcaataa
    47041 taattccacg ttgtgcttac gcaaatagcg ttcagctttt gcagcatcat ttcgatccaa
    47101 catattcaag atcgtcggca ttgcctctac gaccatcagt gtgatttcgt ttggatctaa
    47161 tttataatct ttggctaggc gatttttcca gtcgatcagt tccccaatca tttcaatacc
    47221 ggtaaatcca gaaccacaga caacgaaagt cagcatggct ttacgcagtt catcatctgg
    47281 ttccaatgca gcttttgcta ctgtcttctc aatatgttcc cgaatcctta cagaatcttc
    47341 aaatgaccaa agggtgaatc cgtgtgcttt gacaccaggt gtaccaaagt cattcggctc
    47401 tccgcccatt ccaaggatca gctgatcaaa aggataggaa cctaatttgg tcttgacgat
    47461 ttttcgttct ttatcgatac cgattacagt gtctgtgaca agttgtacat tcttttgacg
    47521 agcaaacaaa tgctgcagat cgtattggat tgcttctggt tctacacgtc cgccagctac
    47581 ttcatgcaac tcggtcatca tagtatggta cgaatgacga tcgatcagag taattgctac
    47641 atcttctttt ttcagtttct ttgccaagta ttttgtagct gaaacaccgg catatcctgc
    47701 tcctacaacg acaatatttt gctttgccat tgatcatccg ctccttatag actatctata
    47761 taatgaatat atgataattc ttttagtttg atacttatta cacaatctcc atatattata
    47821 tagatgttct gttcttttgt ctaactcctt ttggattttt ttcaaatttt aaaataaagc
    47881 gttttttcac gaaatttgct gaaagaaaac gtttgaaata ttacatgggt tatttatcaa
    47941 aaatagttga cttataggct aaaaagatta gaatgtataa tgaatcacaa agaaaaattg
    48001 tggactagaa atggaatggt ggggtattaa aatgaagaaa aaacgttttg taactggatt
    48061 tgccgtatta gcattttcag ctctagtgtt aggcgcttgc ggcgcagatg acaacggaag
    48121 ctcaaactct tcttctgaat catctacagc acaatcttcg actgcaaaaa gcacaacaga
    48181 atcatctgca aaagttgtag ctggcggaga tttgcaagac ggcacatata aattagaaga
    48241 aaaaaattat tcaaatggtt accgtgcagt ctttgaaatg gttgtaaaag acggcaaaat
    48301 cactgaatca aaatatgata acgtcaatga aaacggcgaa tcaaaaacaa aagatgcaga
    48361 atacaacaaa aacatggaag caaaatctgg cacaaaccca gaaaaataca ttcctgagtt
    48421 gaatgaccaa ttcttaaaag cacaaagcgc aagcggtgtg gaagtcgtaa ctggagctac
    48481 tcactcatct gaaagcttcc aaaactacgc acaacaattg atccaagctg ctcaagctgg
    48541 taacacagat acaatcgaaa tcgacaatgg tgctgatttg aaagacggca cttacaagct
    48601 tgaagaaaag aactactcta acggctaccg tgtgcaattc gaaatgacag ttgctggcgg
    48661 caaagtaact gaatcaaact ttgattatat cgacaaagac ggtaaatcaa aacaagatga
    48721 tacagaatat aatgaaaata tgaaagcaaa aagcggtaca gaaccaaaaa cttacatccc
    48781 tactctgaac gacgaattcg tcaaagcaat gggcgaagaa gatggctcac ctgctaatgt
    48841 agaagttgtt acaggtgcta cacacagctc acattcattc atcatgtacg cacaacaatt
    48901 agtgaatgca gctgaaaaag gcgacactca aacaatcgaa gtagataata tcgtaacaga
    48961 aaaataatca cataatcata ctaataaaag ttggagggtg ggacgatagc cagattaggc
    49021 agtcgtctca cctttcaaaa tgaagagagg aaaaagatgc ggaaaaaact actattttcc
    49081 ttaatcacac tgggcatctc ttttatggtg ctaactggct gcagcaactc agcaaataaa
    49141 acaaccactg aaagttcagc agctaccaag atattgaaag atccatattc tgatgaacag
    49201 ttcttgttag ggacttacgt tcgtatccgt gtgtatgatg aaggcaaaga atcagcattg
    49261 aaacctgcct tcgatcgagt aaaagaactt ggtgataaaa tcacgatcaa tcaaaaaggt
    49321 tctgagatcg atgaagtgaa tgagcaagct gggatcaaac cagtaaaagt ttctgatgac
    49381 gtctacacgt tagtcaagcg ggcctacgaa tatagccaag attctcaagg cggattcgat
    49441 atggcgattg gtgcaatcac acaattatgg cgaatcggtt ttgatgacgc cagaaaacct
    49501 tcccaagaag aaatcgatca ggcattgaaa ttagtggatt atcataagat cgaactaaat
    49561 gacaaagaaa aaactgttta tctaaaagaa aaaggaatga tcatcgattt aggtgcaatc
    49621 gccaaaggtt acattacgga cgaagtagtg aaagtcttga aaaaacaagg agtgaccact
    49681 gccatcgttg acttgggcgg taatgtctat gtcttaggcc acagcccaag aggcgaaaac
    49741 caagactgga cagtcggcat ccaagatccg aacatggctc gcggatctgt tttaggttct
    49801 attaaagaac ggaacaaaac attagttacc tctggaattt acgagcgtta tttagaagta
    49861 gatggcaaaa aataccatca tttgtttgat ccaaaaacgg gttatccgtt cgacaatgat
    49921 atcgcaagtg tcacgatcat aacagataaa tcaattgatg gtgacgggtt gtctacagca
    49981 gtcttttcaa tgggtgtgaa aaaaggactg gaatacgtcg aaagcgaatt gaataacggg
    50041 acggaagcga tcttcgtgac aaaagacgat aaagtatatg tgaccgatcc aatcaaagac
    50101 accttcaaat tatcggagga ctcccattac acgatgggtg accgttctga actaaaataa
    50161 ggagtacgaa ccatgacact aaaaatcttt ttagaagtag ttgaaatacg aacaaaggtg
    50221 gctagtgtat ttccttttgc ggtaggggta ttattctcta tcgctttttt tcatgagata
    50281 cattggctaa acactttttt attttttatt ggaatgctag tttttgattt agcgactacc
    50341 gctatcaata attacatgga ttatgaaaag gctcattctg aagtatataa gtatgaagaa
    50401 aatgtcattg gccgcagaaa tctatctcct gtattgattc gaaacatgat tttagctatg
    50461 attgcatttg tttttgttat cggcgttatt ctaacgatca atacgggttg gcttttactt
    50521 ttgatgggat ttatctgttg ttttattgga gtattctata cattcggacc gattccttta
    50581 tctcgaatgc ctttagggga aattttcagt ggggtgacta tgggccttgg aatttttgcc
    50641 atggtcgttt atgtcaatac attcggttca gatgtctatg atctgacagt caacttttca
    50701 tctggacaat ttgatttggt aggaaatctt tgggctatag cagcaatcgt attagcttcc
    50761 ttacccttgg tgtttacgat tgccaatatc atgttggcta ataatttgcg ggatcttgac
    50821 cgtgatattg aaaatcatcg ctatacacta gttttttata tcggacgtcc tgcgggaaca
    50881 gtcttattcc agctattgat gtatgcttgc tatttcgtga ttttaatggg gttgttgagc
    50941 ggtatctatg aatggccgat cttggtcacc ttcttgacct tgccaattgt gaagaagcat
    51001 ttggatcaat tcaagcaaac gttaccccag cctatcagtt tcagctattc catcaaaaat
    51061 atggtcttat tcaatgccag ttatgtattg gggctattac taacaatcat tatgcaagcc
    51121 atttaacagc atgaagaggt tgtgaaaaaa ctgccctgca tcaagtaata agaacagcca
    51181 aataaaaata gctccttata tttttcgttt ggttgggctt attaccgcag atgcaagctt
    51241 ttgaacaccg tttatcagga ggttgtgaaa aagtcgctta gtttcgagta ttaagaaaaa
    51301 ttttgtgaaa atggctttcc acatttttgc ataaatttgg cttaataccg agaaactggc
    51361 ttttgaacac cgtttatcag aggttgtaaa atcagtgttt tgtcctgagt attagagaaa
    51421 caaatggaaa aatagttcct catattttgc cgttttttga gctaataccg aaggacagct
    51481 gattgaacac tatttgttcg gaaaaagtga ctaactatga cacaattttt ttcacagtcc
    51541 cttttttcac ttgatttgat tcgtaccagc gaacttgacg agaactttct tggtttggta
    51601 actatatggt attcttttaa catcctcgtt tgcttcttct aatttattta tacaaagtca
    51661 cactttttta aacatctgtt cttcgataca tttctatttc ttttaaacat cgataccaat
    51721 attttgaact taagagtgca taccaaatgg attcatttac tcttcgtcag ttacgaagat
    51781 aatcaaaatc tccaacttcc ctacttattt cgttttgaga attatcgaag caatcttaca
    51841 taaataacta aaaaacaaaa aaccagaaaa tcagtatact gattttctgg ttttacatgg
    51901 tttgttcaat aaaaagctgt tggaactgtt aatattaaat tatcgcatcg ttacgaattc
    51961 ttccgctcct gttggatgga tggctactgt attgtcaaag tcttctttag ttgcacccat
    52021 cttgatagcc acggcaaaac cttgcagcat ctcatcgaca ccaacaccaa tagcatgtaa
    52081 gccgacgatt ctttcttctt ttccgacaca gatcaatttc atttcgcatt tttggcgata
    52141 atcatttaat gcaaagtaca ttggggtaaa gcgtgaacga taaactttca attcattttc
    52201 gccatatttt tccaatgctt cttcttctgt caaaccaatt gttgcaattg gtggatgcgt
    52261 aaagacgact gtcggaacaa gtttatagtc aagataagaa tctttctttc cattaaataa
    52321 acgttctgat aaacgtctac cagctgcgat tgcaacagga gtcagatcta atttaccgat
    52381 cacatcacca acagcaaaaa tatgttcatc cgttgtattt tggtatttat ctactttgat
    52441 aaaccctttt ttgtccaatt ccacatttgt attttccaga cctaattttt cggtattcgg
    52501 tacacgtcca ccggcaaaaa gtacacaatc accagttaag gtttcaccat tttcaaatag
    52561 caccgtatat tcgtcattgt ttttagtaat ttctttagca atatgatgtg cgtaagtttt
    52621 tattccagct tcttggtaca tttggatcaa attgtccgaa agcattttat caaacgttct
    52681 aagtggtcgt tcttttcgat atgcccacat gacttcacta cctaaaccac gaaatacacc
    52741 tgaaagttca gccgcaatgt atccagcacc taaaacgatt gctctcttag gtaactctgt
    52801 taattcaaaa aatccatttg aatcaattgc gtattctcca ccaggaatgg tcattttact
    52861 tgcacggcct ccagtagcga tcaagatttt aggagcacga taggtttcac cgtttacttc
    52921 gatcgtttga ctatcgataa aagtagcgta accttttata gcctcgacat gattgctatc
    52981 taatcctctt tgataagcac cgtgtagaaa atcaatatat ttttctcgtc tttctactag
    53041 accggcgaaa tcaacatttt ttaaatctgc ttggataccg taacttgagg catctcgttc
    53101 aattgtttcc aaaatcgtac ttgcttgcca cataactttt ttaggtacac aaccgacgtt
    53161 gacacatgtt ccgcccaatt cattcgcttc aatcagcaaa acttttgcgc catgcatccc
    53221 tgctctgttt gctgaagcaa taccgccaga gccgccacca attacgatgt aatcatatat
    53281 ttgcattttg ttcctcctta ttgaagagat caatcaaaac ctttatttaa cgatcctttt
    53341 tctacagaaa acaagtgtaa cacttttctt gaattttgta tgcgaagatg cttaaaacag
    53401 ggatatatca gctttttcct tatttacgtt ttcggatcat atctccaggt ttcaccggct
    53461 gtgcgactgg catagttaaa atcatcattg gatttggtgc tcggtcaatg gattcgcctt
    53521 cttcattatg cataacttca acggtttgac tgaaatgact gaatcctgga ccataaaatt
    53581 cgatttcatc tcctacagaa aacaagtttc tctgacgtat cgtagcaatc tgtgttgcct
    53641 catcataaga taatacttca ccgacaaatt tatatactgg gattttacga cgttcaccga
    53701 ataattgttc gttttcagtt ggtgtattgt agtaaaaacc tgtagctaat tctcgttgtg
    53761 ctactttcca taactcgtcg atccattctt gcttgcagac atagttttca ggatcttcca
    53821 tataactgtc tacagccgct ttatagacat ttgacaccgt agatacataa tggatcgatt
    53881 tcattcttcc ttcgatcttg aagctgtcta cgccgttttc aatcaattct ggaatatgtt
    53941 cgatcattga catatccact gcactcatcg agaattcttc ttccacttcg cctgtatcag
    54001 ttaaactgtt tcgttcttga ccaaaaggca tatcgaacaa tccatacttc cagcgacaag
    54061 attgagaaca tccgccacgg tttgcatccc gcatcgacat atggttggac aatgtgcatc
    54121 gaccagaata agagatacac atggcaccat ggataaatgc ttcgatttcg gtatccgtat
    54181 ttttacgaat ctctgccact tcctccattg aaacttcacg agctaaaacg acacgttcca
    54241 acccttcttc tttccagaat tccaatgtct caaaattagt tgctgaagct tgagtggata
    54301 aatggatcgg cagacctggg gcttctgtcg cacaaatttc gatcaaagct ggatcagaaa
    54361 caatcactgc agaaataccg acatcacgca aactgcggaa aaattcccct gctccatctt
    54421 catttccttc atgggtgacc atattagcag caacgtatac cttcgcatca tgttcttttg
    54481 caaaagcaac tccttcagcc atttcttcat aagtaaaatt acctgcacgg cttcttaaac
    54541 catatgcatt cccgccaata tatactgcat ctgctccata atggatcgcg gtttttaact
    54601 tttccagtgt tcctgctggc gctaggattt ctggtcgttt caattttctt tttactgtca
    54661 tttctttcat catactcctt atttgatagc agaaggatca atataaaaga atcctgtatc
    54721 taattcacgg ttttcaggat gcaatttact gatttcgcta gtcatttttt ccgctttttc
    54781 ttgatcccag ctaccttcta aaatttgttt ttttgcttcg gcgaatagtc gagtgatgtt
    54841 tacgaatttt tcgcctggag taaataatcc gtccaatttc catgtacagt aatgatgctg
    54901 ataaagttca ttcaattcat tcgagagatt cagatcattg ctggcaaaaa tatgtgtgcc
    54961 atgactatct tcataaatag aataatgtgt ctcttctttt ttaggttccg agataaaaag
    55021 acctctttca cgatcttttt gttcgtcttg tttcgtatag ttatagtaat tctgtagcag
    55081 tggacgtttg gattgatgga tgcatgtagc accatacaca agggtttcta caggaatatc
    55141 gagtttttct tccatcgcct tcatctcttc aaaaggaact tcacgagcaa gtactgctcc
    55201 aactgcgcct tttttcgccc agaaattgat ttgacgtgcg ctcgttacta atgtttcagc
    55261 atcataaata tacggtatac gcaattcagg attttttttc atcgtataaa taatacctgt
    55321 atcaccaact gtgatcacat ctacgtttat ttcttttaaa aaagccaaat attcaggtac
    55381 atttttcatt ttttcaggat gaatgatccc atttacagca atattcgctt ttttaccata
    55441 cttgtgtgct aattctacca attgtcgctg ttcttctcgt gtaaatgaag caggcagacg
    55501 caatccaaat tgctcttcac cgaaatatag tgtatctgca cctgcagcaa gcaactgttc
    55561 tgcttgttca atagtttcta ctgtggttat gagttcgatc ataattttct cctcctacaa
    55621 cttcataata gtagcatagc tctaaaaact ttccaatact tcttctattt aaagtcggat
    55681 ttttgaaaat tatttcacaa aaaatcaaaa taaagttcga tatattctgt ccaaatataa
    55741 aaagagattg tgacaaaaat cttgtcacaa cctcttaatc aagtcttact attataaaaa
    55801 ttcagctaca caggccaagt aactataggg aacctactgt ttccttttta ggtccgcgaa
    55861 cattcaatgt gatcttacct tttgaagcac ctactgttac gtggtcacct aaacgaattt
    55921 ctccgccaag caaagcctcg ctcaatcgat cttcaatttc tttttgaagt gcgcgacgga
    55981 ttggacgtgc accatattca ggatcaaatc cagctttccc gatcacatcg attgcagatg
    56041 gtgtgatttt cacttgaata tcttgttctt tcaatcgttt gataatcgct ttgctcatga
    56101 tttggacgat tgtatggatt tcatctttac tcaatgaatg gaagacgaca gtctcatcga
    56161 ttcgattcaa gaattcaggt cggaaagctt tctttaactc ttctaagata cgtttttgca
    56221 tggctttgtg atctttagta acatcttgga cattgaatcc gacatttttt tcttctcgaa
    56281 tttgtgtcgc tccgatattt gaagtcataa tcatgattgt atttctaaag tccacttttc
    56341 tacctttaga atctgttaaa tgaccatcat ccaatacttg cagaagtaaa ttgaaaacgt
    56401 ctggatgtgc tttctccact tcatctaaga gaatcactga atatggtttt tgacgaattt
    56461 tttctgtcaa ctgtccacct tcatcatacc ctacgtatcc tggaggagac ccgattaatc
    56521 ggcttgtact gtatttttcc ataaattctg acatatcaac acggatcagg gcatcttcac
    56581 ttccaaacat gacttctgaa agtgctttgg ccaattctgt ttttccgaca ccagttggtc
    56641 caaggaacat gaatgaacca attggacgat caggatcttt caatccgctt cgtgcacggc
    56701 ggatcgcacg tgaaactgct ttgacagcct catcttgacc aacgacacgt tcatgcagaa
    56761 gtccttccag ttctaacaag cgttcgcttt cttttttctc caactgctgt agagggacac
    56821 cagtccactc cgatacaact gtagcaacat cttcttctgt tacacgatca gaataacctt
    56881 gctcctgttt tgcttcaatt gcagaaactt tttgcagctt tttacgtagt gctttttctt
    56941 gtctgcgtaa ttgagcagca ttttcaaagt cttgtttttg gatcgctgct tccttttctt
    57001 gtactagttc atcgatttct agtttgatca cagttgattt tgttaagtga tcggtttgat
    57061 ccaaacggac tttcgctgct gattcatcga tcaaatcaat tgctttatcg ggtagttgac
    57121 gagaagtgat ataacgaaca gaaagattga ctgctgctcg tacagcttca tctgtgatct
    57181 ccactccatg gtgctcttca tatcttgtac gtaatccttg aagaatcact tctgcttctt
    57241 caggagttgg ctcatctact tgaatgcgag caaaacgtcg ctctaatgca gaatcttttt
    57301 cgatatattt ttgatattca tctagggtcg ttgctccgat cgtttgtaat tctcctcgag
    57361 ctaacgccgg tttcaagata ttcgaagcat caattgctcc ttctgctcca cctgcaccaa
    57421 ttaaggtatg caattcatca atgaataaga ttacttgacc atcattatag atttcgtcga
    57481 tgaccttttt cattcgatct tcaaattcac cgcgatattt cgtaccagca accaaagatc
    57541 ccatatcaag catcatcagg cgcttttctt gcatgtcttc tggcacttca cctaaaatga
    57601 ttttttgagc taacccttca gcaatggctg ttttgcctac accaggttca cccaccagta
    57661 cagggttatt ctttgtccgt cggctaagaa tctggataag ccgtttcact tctctcgaac
    57721 gaccaacaac tgggtccagc ttgttttctt ttgctaattt cgtcaaatca cgtgctaatg
    57781 aatctagcgt aggtgttcct tgcggtgttt gccgtttatc ttgtacattt acatttctac
    57841 gacgattcat tccttttcca gcagaatcac cagctagccc cattttttgt ttcagaactt
    57901 gacgggcttt tgaaaggctg atacctaagt tgatcatgat tcgagtagcc atgatacttt
    57961 catcttttaa taagctcatc aaaatatgtt ctgttccaac tttttgagaa ccagttttct
    58021 tcacttcgct gctagctaaa gcaaaaactt gtttcattct tggtgaatat ggaagaaaga
    58081 ctcctttagg gtaagaacgg ataccaccat aaccaatcag atgttcaatt tcttcataaa
    58141 tttccgtttc attcaaatcc atttcacgta atacttttcc agcaattcca ttttgctcga
    58201 ccactaaggc tagtaaaaca tgttctgaac taactgattg atgtttcaac cgttttgctt
    58261 cttcttgtgc aatttctaat actgcttggg ctctttcagt aaaaagttct tccatctaat
    58321 ctcacttccc atcttcataa cttaaacgtt ccagaaatgc tcggagaatc cgagcacgtg
    58381 tatgatcttc atattcattg aaattcaatg tgttttttgc aattgcactc aacatcaagt
    58441 ttccttcttt ttttgtaatt atttggtctt cataaagttt ttgaataata gcaaaagcat
    58501 ttttctcact gattgtatca tcaaataact gatttatctg ctcaagtaat tgttttttat
    58561 ctgaaatcct tacttttgca attcgtatat aaccaccacc accacgtttg ctttctacta
    58621 gataaccacg ttggatggta aaacgggtat taatcacata gttgatttgt gaaggaacac
    58681 agttaaagag gtctgccatt tcaatccttc gaatctcgat catttcacta ttttccagaa
    58741 ttttctttat atatgattca attaaatccg aggtattctg atgtgccatc ttattcattc
    58801 cctttgacta atattgacct taattataac gaatttgata atagaaagaa agaagaacgc
    58861 tttttgttaa ataaatcctt tagaatcaac aaaaaaaaat acggaatata ctattccgta
    58921 tc
//
LOCUS       DAKBVG010000003        44778 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000003 DAKBVG010000000
VERSION     DAKBVG010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 44778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44778
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.003"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(<1..543)
                     /locus_tag="OUD09_000138"
     CDS             complement(<1..543)
                     /locus_tag="OUD09_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCT9691451.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLK"
     gene            complement(620..1150)
                     /locus_tag="OUD09_000139"
     CDS             complement(620..1150)
                     /locus_tag="OUD09_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HCT9691452.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(1154..2014)
                     /gene="rlmB"
                     /locus_tag="OUD09_000140"
     CDS             complement(1154..2014)
                     /gene="rlmB"
                     /locus_tag="OUD09_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCT9691453.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(2007..2402)
                     /locus_tag="OUD09_000141"
     CDS             complement(2007..2402)
                     /locus_tag="OUD09_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HCT9691454.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(2399..3811)
                     /locus_tag="OUD09_000142"
     CDS             complement(2399..3811)
                     /locus_tag="OUD09_000142"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCT9691455.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(3808..4344)
                     /gene="cysE"
                     /locus_tag="OUD09_000143"
     CDS             complement(3808..4344)
                     /gene="cysE"
                     /locus_tag="OUD09_000143"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCT9691456.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(4430..4669)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(4666..6126)
                     /locus_tag="OUD09_000144"
     CDS             complement(4666..6126)
                     /locus_tag="OUD09_000144"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCT9691457.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(6296..7453)
                     /locus_tag="OUD09_000145"
     CDS             complement(6296..7453)
                     /locus_tag="OUD09_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HCT9691458.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(7487..8857)
                     /gene="radA"
                     /locus_tag="OUD09_000146"
     CDS             complement(7487..8857)
                     /gene="radA"
                     /locus_tag="OUD09_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCT9691459.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(8920..9447)
                     /locus_tag="OUD09_000147"
     CDS             complement(8920..9447)
                     /locus_tag="OUD09_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCT9691460.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            9677..10483
                     /gene="proB"
                     /locus_tag="OUD09_000148"
     CDS             9677..10483
                     /gene="proB"
                     /locus_tag="OUD09_000148"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCT9691461.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            10480..11727
                     /locus_tag="OUD09_000149"
     CDS             10480..11727
                     /locus_tag="OUD09_000149"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCT9691462.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            11803..12147
                     /locus_tag="OUD09_000150"
     CDS             11803..12147
                     /locus_tag="OUD09_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9691463.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(12264..12590)
                     /locus_tag="OUD09_000151"
     CDS             complement(12264..12590)
                     /locus_tag="OUD09_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9691464.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(12963..14164)
                     /locus_tag="OUD09_000152"
                     /pseudo
     CDS             complement(12963..14164)
                     /locus_tag="OUD09_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            14281..14796
                     /locus_tag="OUD09_000153"
     CDS             14281..14796
                     /locus_tag="OUD09_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCT9691465.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(14995..15441)
                     /locus_tag="OUD09_000154"
     CDS             complement(14995..15441)
                     /locus_tag="OUD09_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HCT9691466.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(15488..16966)
                     /gene="msr(C)"
                     /locus_tag="OUD09_000155"
     CDS             complement(15488..16966)
                     /gene="msr(C)"
                     /locus_tag="OUD09_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HCT9691467.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(17320..17949)
                     /locus_tag="OUD09_000156"
     CDS             complement(17320..17949)
                     /locus_tag="OUD09_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HCT9691468.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(17933..18397)
                     /locus_tag="OUD09_000157"
     CDS             complement(17933..18397)
                     /locus_tag="OUD09_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCT9691469.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(18884..20152)
                     /locus_tag="OUD09_000158"
     CDS             complement(18884..20152)
                     /locus_tag="OUD09_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HCT9691470.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(20383..21120)
                     /locus_tag="OUD09_000159"
     CDS             complement(20383..21120)
                     /locus_tag="OUD09_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCT9691471.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(21187..21756)
                     /locus_tag="OUD09_000160"
     CDS             complement(21187..21756)
                     /locus_tag="OUD09_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HCT9691472.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            21884..22756
                     /locus_tag="OUD09_000161"
     CDS             21884..22756
                     /locus_tag="OUD09_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCT9691473.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            23098..23970
                     /locus_tag="OUD09_000162"
     CDS             23098..23970
                     /locus_tag="OUD09_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCT9691474.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            23967..25925
                     /locus_tag="OUD09_000163"
     CDS             23967..25925
                     /locus_tag="OUD09_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCT9691475.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(26176..27027)
                     /locus_tag="OUD09_000164"
     CDS             complement(26176..27027)
                     /locus_tag="OUD09_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCT9691476.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            27150..27617
                     /locus_tag="OUD09_000165"
     CDS             27150..27617
                     /locus_tag="OUD09_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HCT9691477.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(27880..28362)
                     /locus_tag="OUD09_000166"
     CDS             complement(27880..28362)
                     /locus_tag="OUD09_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HCT9691478.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(28462..29325)
                     /locus_tag="OUD09_000167"
     CDS             complement(28462..29325)
                     /locus_tag="OUD09_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCT9691479.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            29923..30771
                     /locus_tag="OUD09_000168"
     CDS             29923..30771
                     /locus_tag="OUD09_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9691480.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(30942..31283)
                     /locus_tag="OUD09_000169"
     CDS             complement(30942..31283)
                     /locus_tag="OUD09_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HCT9691481.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SMEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            31369..32076
                     /locus_tag="OUD09_000170"
     CDS             31369..32076
                     /locus_tag="OUD09_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HCT9691482.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            32063..32386
                     /locus_tag="OUD09_000171"
     CDS             32063..32386
                     /locus_tag="OUD09_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HCT9691483.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(32475..33512)
                     /locus_tag="OUD09_000172"
     CDS             complement(32475..33512)
                     /locus_tag="OUD09_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HCT9691484.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(33959..34942)
                     /locus_tag="OUD09_000173"
     CDS             complement(33959..34942)
                     /locus_tag="OUD09_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HCT9691485.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            complement(35358..36128)
                     /locus_tag="OUD09_000174"
     CDS             complement(35358..36128)
                     /locus_tag="OUD09_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT9691486.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(36121..37056)
                     /locus_tag="OUD09_000175"
     CDS             complement(36121..37056)
                     /locus_tag="OUD09_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT9691487.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(37650..39299)
                     /locus_tag="OUD09_000176"
     CDS             complement(37650..39299)
                     /locus_tag="OUD09_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCT9691488.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            complement(39494..40390)
                     /locus_tag="OUD09_000177"
     CDS             complement(39494..40390)
                     /locus_tag="OUD09_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCT9691489.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(40383..41063)
                     /locus_tag="OUD09_000178"
     CDS             complement(40383..41063)
                     /locus_tag="OUD09_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCT9691490.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(41064..41846)
                     /locus_tag="OUD09_000179"
     CDS             complement(41064..41846)
                     /locus_tag="OUD09_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT9691491.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(41839..42756)
                     /locus_tag="OUD09_000180"
     CDS             complement(41839..42756)
                     /locus_tag="OUD09_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCT9691492.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(42759..43043)
                     /locus_tag="OUD09_000181"
     CDS             complement(42759..43043)
                     /locus_tag="OUD09_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9691493.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            complement(43744..44364)
                     /locus_tag="OUD09_000182"
     CDS             complement(43744..44364)
                     /locus_tag="OUD09_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9691494.1"
                     /translation="MNINRVIKLIGAKNINKVRSEFIFPEDFFNWFFYQFSECKRLLG
                     ENYYLKGIGGFMGNVADEQNIIKGISEQTSELTVTKAFISNGESLKNVTARIRKENEV
                     DIVFGIDHECNSVIYVTQSANLILFENGCKDDFMVVYLYDILIPKLRSLYMSSESIFV
                     SEQKGSFSKKIGIEVIESIMCHNHITLEELEILIGQKNNNTKIQVS"
     gene            complement(44445..>44778)
                     /locus_tag="OUD09_000183"
     CDS             complement(44445..>44778)
                     /locus_tag="OUD09_000183"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9691495.1"
                     /translation="TSTKLICKLFKLDKISNIDDVKSKILTRKVSEPVKLTAQDKKNG
                     IVLYGDDAKYNYNWDTSSEAEVLSIPTTDTSGEKVTFKINFITAISKLEYGKQKKRDN
                     NDQYLPKK"
BASE COUNT        12949 a         9492 c         7967 g        14370 t
ORIGIN      
        1 tttgagtttt actctcccgt aagcgctcat tacttgcttg tgatccattg ctaaacattc
       61 agccatgtct acaaaatcaa cgccttgcat ataataatga aaaattttag cttctatttt
      121 tgaaaactgg atttcgcaat tttcgattgc ttcttttaat aaaagatgct gaatcgctaa
      181 aggttcttgc aatgtgatct ctgctaaaaa gtctggtcct tctgtctcta ttttttggtc
      241 gattgagact tcatcttttt gatcacgacg cttcagcgcg ttctgcttcc tcaacagaga
      301 atagatttga ttgcttataa tgcttttata ataacttcca aagctaacac ctttgctttt
      361 ttcgtaagtt ctcaatgcct gatataaagc gatccttgct tcttgttccc aatcctcaga
      421 aacataattt cttaaataat aggtgttttt caaacgtagt aaaagtggaa aatatttttg
      481 atacagggca gcaaatgctt cttcgtcacc attacgtgca gcgcgcaata actcctcaga
      541 cataaaataa ttcctccata aattcgttta tggaggaatc ttatcatcaa tgattcatcg
      601 aactgattag aacgatatgt tatttatttt ttctcattaa atcgtccaat ttttgggaca
      661 atttttttaa ctgctcttca ttccatggag aattacgtcg gaaatcaata aaatgcatat
      721 ctttattttc ttgggctatt gttttttccg ttttttttat cgtcttatat aattcataag
      781 cagaagttcg taatgctcct tgggaaaaga tcatccactg ttctgctaaa tcgcttgtcg
      841 ccactgtcac ttgtgtcaaa cggtcattca gctgaccagc gattctctcg atataactgt
      901 cagccgtttc gtcttctttc gtaaaaacaa catccaactg gtatttctta taattttgtt
      961 ggatgcccgg aactagttgg gcatcgaaga caacgatgac ttccattcct tcatatttcg
     1021 cataatttga taaacgatgt aaaagagctt ctcttgcatc cgccattttg tcctgctttt
     1081 taagctggac aagttccggc cacgaaccaa tcatgttgta gccgtctaca atcaacagct
     1141 gttttttcat ggtctactcc ccctgacgtt gggaaaaagc ctgatacata agcaaaccag
     1201 cagcaacact ggcatttaag ctctggacgt gcccggtcat cgggatcgtc agcaattcat
     1261 ccatttcttt tttcaatcct ggactcattc ctttgccttc attaccaatg actaaagcaa
     1321 ttgatccttt ggcattccat ttttggaagt ttgtgccggt catatctgtg ccaaataccc
     1381 aaaatcctgt ttctttgagc tgttgcacag cttgtaccaa attcgtcacg cgtgcaacag
     1441 ggacatgttc gactgcccca gtggaagtct ttgttacgat aggtgtgatt ccaactgctc
     1501 ggtgttttgg tatgatcaca ccatcgacgc cacttgcgtc tgctgtccgt aaaatcgacc
     1561 caaaattgtg tggatcttcg atgctatcca aaatcaaaaa gaaggggtct tcttttttct
     1621 cttttgttcg ttcaatcagt tggtcaaggg ataaatattc ataaggtgta atcgcaagaa
     1681 cgactccttg atgcacgccg tgatcgctca aagtttctaa tttttgttta ggaacccatt
     1741 tgactggcac agcttgttca cgtgcgattt ctttcagttc ttctactttt tctcctcgtg
     1801 aatcttcttg aagaaagagt ttattccctc gcccttgttg tagcgcttct actgcggcat
     1861 ggtgcccaaa gacgaaatta tcttctaatg cttcttcatt ttgatcagaa actgactgat
     1921 ttcgtccagt ttgcttttta tttgaatatc tattttgttt tttgtcttca aatccacgtt
     1981 tcgattgatg tttgtttgtt ctttctttac ccattttttt caccaacttt ctgaatacac
     2041 cagcggatca attcttccat ccgctcggtt tgttttgtca aatgcagata gcccatgagc
     2101 gcttcgaacc ctgttgcgac ccgatacgtc gtaacatcag cattttttgc gctcgtatgg
     2161 cttttagcat ttcttcctct gcgataataa agttcttctt cttctgtcaa aagatgttct
     2221 tccagcattt gctgaatcaa aaaggcttgt gcattcgcag aaacataatt cgttgccgca
     2281 cgatgcagct tattgggttt tgtttctcct tgatcaatca ggtggtcacg aacatagatt
     2341 tcgtaaatcg catctccaac ataagccaag gctaaaccgt tcaattgtgt gtaatctctc
     2401 atgcttctct tctccatcgg gttccttgcg gtgtatcttc taataagatc cctttttctt
     2461 tcaacaaatc acgaatttcg tcgcttcgga caaaatcacg attgcttctt gcttgatttc
     2521 gttcttcgat caattgttca atctcttcat ctaataactc ttctgctgta aataaaatac
     2581 cgaagatcga aagccattgc gagaataaag cttctccttg tgccaagaca gcggcagaaa
     2641 ctttcggctg gtctagataa gtgttcagcc acttagcaag ctcatagacc actgtgatcc
     2701 catttgctgc attaaaatca tcgtccattt cttcgatgaa tcgctgttct aaattttcta
     2761 acaaagcgat tttatccgca tcatctgcca aattctctga ggcagtttct tggcggaatt
     2821 tcaaattgtc aaacgtattc ttcagtcgtt gtaaattggt ggctgcttct ttcatcgtcg
     2881 tttcactgta gcggatcgga cgacggtatt gtgtggtcgc catgaagaaa cgcaagactt
     2941 gtggatcaac gtttttgatc atttcatgta cagtgatgaa attccctaga gatttactca
     3001 ttttttcatc gtcttcgcca atcgttacat agccattgtg catccagtaa ttagcgaatt
     3061 ttttccctgt tttcgcttca ctttgtgcga tttcattttc atgatgagga aattctaaat
     3121 cttgcccgcc accatggata tcgatcgttt ctcctaaatg ttttgttgcc ataacggaac
     3181 attcaatatg ccaaccggga cgtcctttgc cccatggtga atcccaagaa atctcgccag
     3241 gttttgcttg tttccataaa gcgaaatcta atgggtcttc tttttgctgc tgttcttccc
     3301 ctgtacgctg acttgctccg acttccagtt catcgatgga ttggtcactt aatttcccat
     3361 aattttcaaa tttacgggta cggtaataca catcacctga agattcataa gcatagcctt
     3421 tttcaaccaa cacttgaata aatgctaaaa tatctggcat atgatccatg actcttggat
     3481 gcagtgtagc aggctcgaca ttcaacgctt tcgtatcttc ttcaaatgct cggatgaaac
     3541 gatcagcaac ttctggtgct gtaataccta attcgtttgc tgtacggatg attttatcgt
     3601 ctacatccgt gaaattcgat acgaaatcga ctttatagcc tcgatattca aaatagcgtc
     3661 gaattgtatc gaaagcaatg gtgctacgag catttccgat atggatataa ttatacacag
     3721 tcggtccgca gacatacata ccgacatgtc ccttttgtat agggatgaat tcttcttttt
     3781 ttctggtcaa tgtattgtag atttttatca ttctgatctc ctcacttttt ctcctttgat
     3841 tcgaacgact tttgctggta ctcctaccgc tgtagcgtcg tctggaacat ctttcaacac
     3901 aacggcacct gcaccgattt tggcgttttt tccgatcgtt accggtccta atatctgcgc
     3961 attcgcagag agcatggctc cttgtttaac agttgggtgt cttttacccg tttcttttcc
     4021 tgtgccgcct aatgttacgc catggaataa aatcacatcg tcctcgattt ctgcggtttc
     4081 gccaatgacg attcccattc catggtcgat gaacacgcca gttccaatcg ttgctcctgg
     4141 atggatctca acaccagtta ggaaacgcca gaattgagca ttgatcttcg ccaataaaaa
     4201 gagccggtgc tgatataaaa aatgagagaa acgatgccaa aacaaggcat gaaaaccagg
     4261 gtatgtcaaa acgatctcag ctgtcgaacg tgcagccgga tcgtttctct ttgctgcatc
     4321 aatcgctcgg gtaaaccaac tcatgacttt ccactccttt cgtaagaatt ttatgtagga
     4381 aatcatcttc ttataaagaa aagaagcgcc ttttaaataa gtttccttat caaaagacgc
     4441 tttccgcgtg gttccacttt tcttcataat caatgattat ctctgacagc taacgcctgt
     4501 tatacgttct tggctacttt gtttcctata aaacagtttt tcctgttttc tgtgtttcgc
     4561 caagaccact cccagaggca tttcggcaac cattcattga taccttgcac cacccggtat
     4621 ctctcttgta atgatcagct gcttactttt ttctgttcat cgtgtttagt tcagtacttg
     4681 atttaaatgt gctaatgatt tttcacgacc aagcaattcg atcgtttcag gcaattctgg
     4741 accatgcatt tgtcctgata cagctacacg gattggcatg aaaagatttt ttccttttac
     4801 gccagtttct ttttgaacag ctttgatggc agctttgaca gttggagcat ctactacttc
     4861 catttgttct agctgtgctt tgaatgcgtg gagaacagta ggaactgttt caccagcaag
     4921 tacttctttt gctgcttcat tcaatacagg atgttcattg aaaaatagcg tagacagatc
     4981 aacgatttct gcagcatagc tcatttgagg ttgatacagg ctaacgatct tcttcaccca
     5041 ttcgattgtc ttttcatctg ggttttctgc tacacgtcca tcagcgatta gataaggcag
     5101 acacatgtca gtaaatgcat ctagatccat tgctttgatg taatggttgt ttacccattg
     5161 cagttttttc gcatcgaaag ctgcgggtga tttacttagg cgttttggat cgaacatttt
     5221 gatcaattct tggtgttcga agatttcgtc ttcgcccaca ggtgaccagc ccaataatgc
     5281 aataaagttg aacattgcat ctggtagata gcctaattcg cgatattgtt cgataaattg
     5341 caagattgat tcgtcacgtt tgcttagttt tttacctgtt tcactgttga tgatcaatgt
     5401 catatgtccg aaggttggtg gtgtccagcc aaatgcttca tagatcatca gttgttttgg
     5461 tgtattggca atatggtcat ctccacgtaa aacgtgagtg attttcatca aatgatcatc
     5521 gaccgcaaca gcaaaattat aagttggcat accatcgcgt ttttgtatga caaagtcgcc
     5581 accgatatta tcggattcga acgaaatagc gcctttcacc atatctgtaa atgcgtattc
     5641 tgtattgcga ggcacacgga aacgtacaac agattctaga ccttgtgctt ctttttctgc
     5701 ttgttcttca ggtgtcaagt tcgcacatgt tcctgcgtaa tgaggcattt ctcctcttgc
     5761 gcgttgtgct tcccgttctg cttctaattc ttcttctgtg caatagcatt tgtatgctcg
     5821 attgctagca agcagctgat caatcagcgg ttgatagatt tctttacgtt cagattgacg
     5881 gtaagggccg tattctccag gattttcagg agattcatcc caattcattc ccaaccaagc
     5941 aaggttttcc aactggcttt tttcgccatc ttcgatatta cgtttttggt cagtatcctc
     6001 aatacgaata ataaagtctc catcattgtg gcgggcaaac aaataattga ataatgcagt
     6061 tcttgcattt ccaatatgca ggtgcccagt tggacttggt gcatagcgta cgcgtacttt
     6121 cgtcattttc ttcctcttct ttcaaactag actttgacta acagcaaaaa ttccgtcagt
     6181 tacggcttct caaatatcat ttatttaaag ccactcagca caaattttac cttttctaag
     6241 gtaaatagta aagccaactg gttgaatttc cttaccagtt ggctgaattt cttttttatt
     6301 ttgattgttt agtctttttc tctgtctctt ttttattgtc gattcctcgt cctgcatgtg
     6361 caggtttagc aaagatcatg cgtccagcag ctgtttgaag ggcacttgtt acaacgactt
     6421 cgatacgttc attcatgtaa tgctgtccat cttctacgac aaccatcgtg ccgtcatcaa
     6481 ggtaggctac tccttgttgg cgttctgtcc cggcttttac tactagaaca ttcatcgttt
     6541 caccagggat gacgactggt tttacggcat ttgccaacgc attgatatta agaacaggta
     6601 cattttggaa ttcagagact ttattcaagt tatagtcatt ggttacaatg acaccatcta
     6661 aaagcttagc caatttgatc aatttgctgt ctacttcact gatatcttca aagtctccat
     6721 cgtacatttc gacagatata ccctcttctt tttgcaaggc atttaggata tctaatccgc
     6781 gacgaccacg cacacgtttc aaactatccc ctgagtccgc gatgtattgt agttcgtaaa
     6841 gaacaaaatt agggatcatc aaggttcctt ctaagaatcc agtctttgta atatcataaa
     6901 tccgaccgtc aatgatcacg cttgtatcca agattttata tttatggaaa ttttctcctg
     6961 attttcgttc taacagttga ctctcaactt gactagctgt ttcttgctcg atttttttgc
     7021 ttcgtgaacc aaaaactttc ttccattctt cgattcgtgt cgttcccatt ctcaatccca
     7081 gatagcctaa gatgatcatc agtaaaatcg gcaatacact attgacgaat ggaatttcca
     7141 tattgtaaag aggggtcgag atcacaacac ctaaagtcaa tccgatgatc gcaccgatcg
     7201 caccgaagag aagataagtc aagctgacct cacttagttt ttgttctact tgtttgataa
     7261 tagctgagat atgtttggcc aacagcaaag ataataaaaa gaaaataagt gcgcctaaca
     7321 ggctgttggt aaatgtatta ttcagccatc cgtttgactg ttgaccgatt gcctgccaag
     7381 ctgagggtaa aaatgaaata cccaagcttg cgccgacaat gatcatcagc aaagtaatga
     7441 cacgtttttg catcgttttt cctccgtttc gacaatcatt tattagttag cggaacacac
     7501 gcttcaacgt ttcagccaaa gttgctactc caactatctc gattcctttt ggcgcttccc
     7561 agttacctaa gttgttttta ggtaggtata cttttgtgaa gccgagtttt tggacttctt
     7621 tgactcgttg ttcaatatga ctgacacgtc tgatttctcc agtcagtcca atttcaccga
     7681 tgaaacattc cgttggctgt gttccttttt ctttatagct tgaagcaata ctgacagcga
     7741 tagctaaatc aatcgctggt tcatttaatt ttaccccacc tgcagctttt aagtaagcat
     7801 cttgattctg aagaagcaac ccagctcttt tttccagcac tgccatgatc agagataccc
     7861 gattgaaatc aagacctgtt gttgttcgtt tggcatttcc gaacatagta ggtgtgacta
     7921 atgcttggat ctctaccaaa attggacgtg tcccttccat tgccacaacg atagcagagc
     7981 cagttgcacc gtctaggcgt tcttctaaga aaatctgtga aggattagcg acttcttcca
     8041 atccatgctc atgcatttca aaaatcccaa tctcattcgt tgagccaaat cggtttttta
     8101 ctgctcgcaa aatccgaaaa ctatgatgtt gttcaccttc aaaatataac accgtatcca
     8161 ccatatgttc taacattctt gggccggcga tcgagccttc tttggtaaca tgaccgacga
     8221 taaaaatggc tatcccattt gttttagcga ttttgagtag ttctgcagtg gtttctctta
     8281 cttggctgac acttcctgcc acacttgtga tgtctggctg cgtcatcgtt tggatggaat
     8341 caatgataac ataatcaggg gagatatgtt cgatggcacg actgatttcg ttcatatctg
     8401 tttcagcgta tagataaaat tctgtgttga tacttccgag gcgttccgcg cgtaatttta
     8461 tctgctctgc gctttcttcg ccggatacat ataagacttt tccgcctatg gctgctaatt
     8521 gctgagaaac ttgtaacagc aaagtggatt tcccgatacc tggatcgcca ccaatcaaga
     8581 ccatagaccc tggcaccaca cctccgccta acacacggtt cagttcttct aattttgttt
     8641 tgatccgagg ttctttttta gggacgacgt ctgcgatttt tgttggtttt gctttttctc
     8701 ctgtcaaact tgtccgagtt ctgcggtccg ttgtatcttg ttcgatttct tcgaccatcg
     8761 tattccattt tccgcaattt gggcagcgtc ccaagaactt aggcgacaca tacccgcaat
     8821 tttggcaaac aaattgtact tttgattttt ttgccattgt accgcctcct tttatcctaa
     8881 tcatgagaca ttaaatcaag cttaagtatc tttaacgttt tatttactgg aagaaccaaa
     8941 tccgccttga cgttgagcag tcgtttgatc ttgatctgct tttaaaaacg gcaaaaagat
     9001 cccttggccg atacgctcgc cttttttgat cactacatcc ttgaaaccaa agttggtgaa
     9061 ttggaacatg atatggcctt catttgcctc attattgtaa taatcactgt caatcacacc
     9121 tactccgttt gtcaacacta aaaaccgctt caacggattg cttgaacggt tagcaagctg
     9181 caaaaattca tcttctccca tgtaagcttt gattccagta ggcacgaggg ttggtttcaa
     9241 ttgtttttca ttttctgata catctttttt caatccatct gcgactgttt ccaacccctt
     9301 tttccagcct gctttccaga tacttggaat cacgacctct tctgctgctt caaaatcata
     9361 gccagcagca tgatgtgtag cgcgctgcgg tagattgata ttttgatctt tatatttaga
     9421 aataacttca aatcctcttt ttctcaccgg ttttcctccc taaataaact actcctattc
     9481 taacatatcc gaggctgtaa aagactttct ttacaaattc ttcaagaaat tctgctatct
     9541 taaagtaaac cattaattcg ttctttttcc aaacgactgc aagttgtaca aaaaaactat
     9601 tttttacatg actttactcg tcaaaacaag tagaatagaa cgaaattaat gagtggatat
     9661 ttgattgagg tgtcacatgc gtaaagaaat taaaaatgca aaacgaatta ttgtcaaagt
     9721 aggaacaagt acattgattt atcccaatgg aaatatcaat cttgcggcaa tcgatcagtt
     9781 agcttttgtc ctttctgatc ttagaaatcg gggaaaggaa gtgatcttag tctcatcagg
     9841 agctgtaggc gttggtatgc ataaattaca tttgtcagaa cgtccgactt tgattcctga
     9901 acagcaagct gcggcggctg ttggtcaagc cgaactgatg aatatctata atcaaagatt
     9961 cagtgtatat aatcaacaga cggcacagat cctcgtgaca agagacgtgg tagattatcc
    10021 tgaaagtcgt aaaaatgtga ggaatacatt ggaacagctt ttgattatgg acacgatccc
    10081 tatcgtgaac gaaaacgata ctgtagcagt ggatgaatta gatcatctga caaaatttgg
    10141 cgataatgat cagctgtcag ccatcatggc aaaacttgtc gcggcagacc ttttgatcgt
    10201 cctttctgat atcgacgggt tttattcaga taatccgact acaaatcccg aggctcagat
    10261 gtatcgctat atcgataaaa tcgatgaaaa gattctggca caagcaggtg gtgctggtag
    10321 cacatttgga acaggcggta tgcagagtaa attaaaagcc gcttctagga tatttgaagc
    10381 aaacagcgca atgattttag caaatggaaa aaatccggca attatttttg atattcttgc
    10441 aggaaaagaa gtaggtactt tatttaagga ggacttgaaa tgactgactt gatgcaatta
    10501 ggacaacagg caaaagatag cgcgcaacaa ctagcactta tgcctacaaa ccgcaaaaat
    10561 gagctacttg ttcaaatggc acaaaccatc aaaaagaatc aacaagcgat cattgaagca
    10621 aatcataaag acttagaaaa agcagtcgaa aacaatatca gtgaaacgat gcaagaccgc
    10681 ttgcagctga cacctgagcg aatcaatgcc atggctacag agatcgaaaa aattgcttcg
    10741 ttagacgatc ctattggaaa agtagatgag atgtggacga acaccgacgg attgagaatc
    10801 ggcaaaaagc gcgtgccact aggcgtgacc ggcatcattt acgaatctcg tccaaatgtc
    10861 acgacagatg cagctagtct agcattcaaa tcagggaatg ccgttatcct tcgcggtggc
    10921 aaagaagcat tcttctcgaa tcaattatta gtccaattat tgcagcaagt actggtcagt
    10981 gctggcgaat ctccttatgc catccagttt gtcgatgata cttctcatga aactgcagga
    11041 cagttgatgc gattgacaga atacttagat gtgctgattc ctcgaggcgg agcaaaatta
    11101 atccaacgag tcaaagaaca ggcaacggta cctatcattg aaacaggtac aggaaataat
    11161 cacgtatata tcgataaaga agcacagtta tctatggcag tgaacatcat cgtcaatgca
    11221 aaagcttccc gtccatctgt ctgtaacgcg gcagaaacat tgctgatcca ttctgagatc
    11281 gcaccttttt tcttgccagc tattgaaaaa gaactggtag aacacggggt ctctttacgt
    11341 gcggatgctc gtgcgttaga atatctagag acggctgctt tagcagaaga cacagattgg
    11401 gatactgaat atctagatta tatccttgct gtcaaagtgg tcgattcttt ggatgaagca
    11461 attgcacata tcaatcggca taatactaaa cattcagaaa cgatcgtgac agatagttat
    11521 gcggctagtc aacgtttctt aaatgaagtg gacgcggcgg ctgtctatgt caatgcctcc
    11581 actcgtttta cagacggatc ggtatttggt tttggtgcag agattggtat ctctactcaa
    11641 aagttgcatg cccgtggtcc aatggggtta aatgaattaa cttccacaaa atatatcatt
    11701 tatggcgatg gacaaatccg cgaataatgt ttcgtgtgaa acattattcc cgccttttgc
    11761 cttttttctg taaaggttct atccaactag aaaggtgtca atatgtttga aattttaatg
    11821 atttttttta tttaccttat ttctctgaat atcgctgctt ttctgggagt cggtatttta
    11881 tcccttttct ttcaattcaa aaaacgaagc atagggagtc aacgggaaaa atggtcgcag
    11941 tattttgaca aaatcgggca aaaagggctg gttacacgct tgcacattag ttacatggtt
    12001 gcattatgct tattagcgac aatcaattac tattcttttt tcgatcattc aatcgcttat
    12061 acgattacgc tactgattgc tgggattttt cacttatctt ataaatatca gctgaataaa
    12121 aaccacttga atcggacttt ccgctaaaaa atatccgtac gataagtaga gtgccttgaa
    12181 gatcggcaaa aaactgccgg actttttaga ggctgtgaca aagattttgt cacagcctct
    12241 ttttccatat aaacggcgtt cgcctaccag ctgcctggta aatctgtttc ctcttcgtta
    12301 agaagcgaaa atccgtcaaa tgtttcgcca tcataataac gaagcagctc atccatggac
    12361 aagttcatcg ttttttcttt ttcttcttcc gctgattcag ccagtagctg ttcccgatac
    12421 acctgataca taccagctat tttttgctgc atcgcttttc tagtttcacc ttccgcattg
    12481 attattggca attctttgat atagccacga taaatcaacc gatcatctga aaattcaatg
    12541 ggtaattcaa cgagtgtcaa cccgacttcc tgaaaatatt cttccagcat ctattatccc
    12601 cccattcttt ttcttaaatg aattttagct tacctactga aaattttcca gtttcattat
    12661 tttgagaaaa aaagggaatg tgacaaaaat cgtgtcacat cccctttttc cgaataaatg
    12721 gtgttcttct gccaactcct caatcataag tcacaatatt caacaaacat gagaaactgt
    12781 tttttgaaat tgttccttgt gattcagagt taaacgacag tctcacaacc tcttggtaaa
    12841 cggcgttcaa aagcttgcat ctgcggtaat aagcccaacc aaacgaaaaa tatgagaagc
    12901 tatttttgtt tggctgttct tatgacttga tgcaggacag ctttttcaca acctctgatt
    12961 tcttattctt cttcatttgt agcaaaataa ttacggtgat aaatgataca acatacactg
    13021 ataagaacaa tggcacctgc aaaataaaac gcatagcttt caccaatatg ttcaccaatc
    13081 acacctgcaa taaatggtgc taaagctcca ccgaaaaagc ggacaaaact atagctggac
    13141 gaagcaacgt ttcgttgcag acctggtatt tccatagcaa tcgtcgtcaa cagtgtattg
    13201 ataattcctt ggaaaaatcc tgctaacaca atgctgatcg gtacaaggac tggcatagaa
    13261 gttccagctc ctaataacag caaacaaata atgaataaga caaatgctgc taaaattgtc
    13321 cgataagtcg taaacttccg ctcaagagcc ggtgcaacga aaatcgaagc aatagccaga
    13381 agcacacccc agccaaaaaa tacaaaaccg acttctaatt cagaatagcc aatcaataaa
    13441 aatggcgcat acgcaagtaa agtaaagaat ccgaaattat ataacaatgc gattagtcca
    13501 actgaacgaa gtttatgatt gcttaacgct ttgacacctt cccaaaggcc tacttttctc
    13561 tttggtttct cagtttcagg taaaagtacc gctagggcta taaaaccgca aaacatcaaa
    13621 aatgctaccc caaaaaatgg ataacgccat gaaccactgc ccaagacccc accgactaga
    13681 gggccaacag ccattccgca gccaagtgct gcttcataca tcatgattgc tttttctgtg
    13741 ttaccaacta aaatactgac gatcgttgct aaagcagtag agacaaataa agcattcccg
    13801 acaccccagc cggcacgtaa cccaatcagc attccgatat tcccagataa tccgccaaga
    13861 ccagcgaata caacgatgat aaacaatcct gtcatcaatg ttttttcaaa ccgatccgac
    13921 tagataagaa acctgtaaat agcatcacca caccagtgac taacatgtaa ctagtaaata
    13981 acagggtcgt ttcagctggc gtagcgttta actgtgcagc aatcgttttt aagattgggt
    14041 ctactagacc cactcccata aaagcgatca tacaggtagc tgctacaact agcgctgaac
    14101 ttttcccctt tgtttcttca tttgtttgtt cttgtatatc tatttgattc tccaatattt
    14161 ccatcccttt ctctctatta gcatcataca tgatttattt tacatgtaaa tatgcacatg
    14221 taaaacgtgg aaactttact gtgatataat aaagaaaata agacgagagg taggattttt
    14281 atgaatactt tagcctatat attattatgc gtgcttttat ccaaaccacg ttctgggtat
    14341 gaattgaagc agctgatcac aattttttgg gaagcccatc acagtcagat ctatacaacg
    14401 cttgccaagt tagaccagca gggttatgtg accgttttgg acaaagacga gcactctcaa
    14461 aaaaagatct accacttgac tgatgaagga cgtgctcttg tagaggaatg gatcaaagag
    14521 gaaactcccg ctcctacgca aaaagatgaa tttctagcaa aaatctatgc tttttcgacg
    14581 ctcgataaaa caacagctac tggactgcta ttcactagag aacaacagct gaacaaaatc
    14641 atgcaaaaaa atcaaaaaaa attgaatgac ctctcgctag acaagaaaca agaatttgga
    14701 cgttatgttg tgatccagcg cagaattatg ctttgtcagc aggaactcca atggtgtcag
    14761 caagtaagag aacaaatgca gcagtttttt gagtaaggga taaacagaac ctttctagaa
    14821 atatctattg aagagcaaat tatcttcaat aaaagagcaa tacagttgga aaagaaaaag
    14881 cgatccgaac gacaggccaa aacctatcgt tcggatcact atttttctag aactgcattc
    14941 aagagactat gacaaaaagc atgccaaggc cttttttcaa ataacagtgt tccatcattc
    15001 attactttga attgctttta gcacacttct tgaaagcggg ccaacgatga tcaactgcaa
    15061 cggtaaagcg gcaatcacat tcattttcca agcattcata taagcagaga ataattgatc
    15121 aggtattcct tgttccatga tcacaccgaa taaagacata catgtcacca ttcctaaaac
    15181 catcaagcat gaaatagtta agatcatttg gaccttgctt tctggattta ctggtagttt
    15241 aaaggcaatt tttttcgcca gcacaccgat gatgaagaca tccaaaatga aagccaccac
    15301 gaatccaggc actagaccaa tcaacaaact tgataaatga agatcttgat gcagccataa
    15361 attatacgcg ctcatcccca agaccatcaa aaaacacatc attgttgtaa aaattattcc
    15421 ttctttttta tttgtaggca atctgtacca ctcctttaaa tttgtcacga aaatcatcgt
    15481 agcacattta aaaatttctc gtaagtactt tttttatttt ctgccctttt atttcataaa
    15541 cttcatcagc cgtacgctcg acaaagtaag aatcatgaga agtaaacaaa atcgttcccg
    15601 gatatatttg cattagcttc tctaaagctt cgatcgttgc catatcaata aaattagtcg
    15661 gttcatctaa cagcaacaca ttacttggct tagtaaaaag caaagcaatc gtcagacggg
    15721 tcgcttctcc cccacttaat gtacataacg gtttcgtcaa agtttcctga gcaaacccta
    15781 agtgattcag caaactacgg acaagcgatt ctgaaaactc tgtttgtctc atgacaaaac
    15841 gaatgatgga ttcttcagat gtcatttgat aacccttttg ctgatatact tgaaagctta
    15901 ctttcggaga gagaaggatt ccttctcctt gtttgcggat atgttctaat aaggttgtct
    15961 tacctgaacc attttcaccg acaatcgcga tttttttacc taaagaaaac tgaaaatcac
    16021 catttaccag taatgttctc ccacttcttt ccaattggac attttgtccc atgatgggat
    16081 agcggctgtg gagttcaaga gattttggta ctggaaaacg gatttgtttc atctgctgcg
    16141 gcttggttgt ttctcctatt ttttggagtc ttctctctaa tgttttcgca ttcttttgga
    16201 tggctttttg tacactgtct ttttgtttag aggaagacaa ccgatccggt ctgatttgtt
    16261 gctttttctt tttactggac actttttcca acttttccgc ttgttttttc ttttcttgga
    16321 tcgatttttc caatcgtttt ttctctttca tgatcgactc tgcttctcgt agctgagtct
    16381 ttttctccaa ttccttttga gtgagatagg cactataatt ccccgaaaac tctcggattt
    16441 ctccgtcttt tacttcccag attttcgatg ctagctcatc tagaaaatag cgatcatgac
    16501 taacaaaaac cagcgtacca taataataac gaagctcttc aatcagtcga tcaatacttt
    16561 ttctatctag atgggttgtt ggctcgtcta gaagcatccc ttgttcataa gaagaaagaa
    16621 gacgtatcaa ctcgatcttt cgttcttccc ctccgctcca tttccgctct gaagtttctt
    16681 ccagtcttaa ctggaaatga ctggctaatt ccaaatttag gtcctttgct tcagcgagat
    16741 aggttaattg aggcaggtag ttgaaggtga tctctgtttg gattttccca tgatcgggaa
    16801 aaagttcacc ggctattagt ttgagcaaag tcgatttacc gacaccattt tctcctatga
    16861 tccctatacg atcttgctga tagacacgaa gagaatcaat agatagttct agctggttgc
    16921 caaagctgac ttgtagattt gttatattta ctgctaaatt ttccatatgt tttacactcc
    16981 tatttcatgt aaaacaacaa ttatcgatac taaaaaagag cagaaaaatc tgtttgcaaa
    17041 aaaactgcat cacatctttt cctactctga acgaaataac tatgactaaa tcaccaaatc
    17101 tgtcatagct ttcacctatt ctactgtcgt tcgaaaaaat ggacatactc catccatgaa
    17161 attgttgttt atacatgtct tttgaattta tttagttgtt attcaaaagt tcgaaacgta
    17221 atttcatcga tgcagtcatt tttccgacct ccttttttat taggttacca cacacgattc
    17281 aaaaaaagca atagctactg aatagtaact gaaaaccttt tattcgaacg tagtgatctt
    17341 ttctggtgct aaacgaacag actcgtaacc ttcttctacc gcttttccta ttgataaaat
    17401 aataactgga acataacggt ctttatccaa accaaatgcc tctgccaact gatctgcttc
    17461 gaatccacca attggatttg tttcatagcc atgatcacgc gcaactaaca tgaattgcat
    17521 agccgctaag ctagaatcga ctttgactac atcgttcatt tcttctcttg aaaaactctt
    17581 gtaataagga atgatcgcag ctaattgttg atctctgact tcttttggca tcttccctga
    17641 ttcgtaagct tggttataga tttcttcgcc atattcgtaa cattgcatat cgccaaaaat
    17701 catgaccatt gcagaagaag tatcattttg tctcgtattg aaacggatca aaggacgcaa
    17761 gatttctttt gcttcctcgc tttcagcgac cacaaagcgc catggttgca tattgacaga
    17821 agaaggtgca gtcgtcgctt ctttgatcat ttccagcatt tcttcgtgtg ggattttgta
    17881 tgtttcatca tatacgcgaa cggatcttct accagtcata attgttgaaa aatcattttt
    17941 ttgttccatt attattaacc tctttcgttt ctgttgttat attgcaaagt tgggtattca
    18001 atttatctaa aagtgttaga aattgtttca tttctacatc gctgaaagcc ggggatagca
    18061 cggaacctgt atctttatgg tccatttcac aatgctcgat tttcttctgg gctttttctg
    18121 ttagcgctac atacacttcc cggttgttct gaggatttct ctgacgcgaa acatagccat
    18181 ttgcttccaa taccttcaga tgacgagtaa ctgctccttg atcaatctgc aacttttctt
    18241 gcaaactgct ttgtagcaat ggattttctt ctttcagtct catcatgatc tcgtatctag
    18301 taagacttat accgatattt tcttcaaaga tctggacgat tttttgatcg gctaatttga
    18361 tttgatataa aaggtgacta atctcttcta aagacataat ttcctccttt ctaataattg
    18421 actcgtcaac gattgacttg tcaataatag aataaaaaaa gaaaaaagtc aaagaatcag
    18481 ctgtgtaata aaaaaaacgc tctcaataga aaagtataga gaaaagaaat tttttgtaga
    18541 atgaacggaa aaaatgataa agaaagcaca acgctaaaac caagattatt gattcttatc
    18601 aaactataga agatagctct tctgcagtag aaaattttac accgccaaaa atttccgaaa
    18661 tcgccttttg caagaatccc gtcgaatatt agaaattgga tcagtatgct taaacagtaa
    18721 taaaaaagtt atgacaagat gttgtcacaa ccttttttga gtaaacggtg ttcaaaaact
    18781 tgcatctgcg gtaataagtt caaccaaaca aaaaacatga aaatctgttt ttgtttggct
    18841 gttcttacta cttgatgcag ggcagctttt tcactacctc tttttaataa ccataaaatc
    18901 ctattccttc atatctccag cccatcggtg ttagccaatc tctttcctta gcactcaagg
    18961 tatagtgatg ggtaccagtt ttttcatttg gattaaatag tcgataaatt ggcttggaac
    19021 caccagaata aaaacttaga ccttcataac gccaaccgat ttttgttaaa gagttgatct
    19081 cagattgtgc cattgtgtaa tgatggtctc cactgtaagg attgtataaa cggtagacag
    19141 gttgtccgct gtttggtgct tgccaagcaa tcccttcata ttcccagcct acagttggca
    19201 gataattttt ttctccggca ttcaatgtat ataaatgttc tcctgtatga cggttataca
    19261 gacgatacat cggagaagtt gaagttggtg ctgtatcata ctgtgtcaga ttataggttt
    19321 cgattacatt gttcaatgta gaaccataat taggcgcagt ggcataacga cctgttagcc
    19381 aagccgtagc atctttgtaa gagttcgtat tgcttttcca tgcaccggaa tagtagtaaa
    19441 cgcctggctg aaaagaagtc gtcttcaata ccgttacgtt atcttgcaat gattctttgt
    19501 acgaaggata ttttctaaaa gcggcattca ccgtaatcca ttgcccattc acatattccg
    19561 aagtaggcat cgtaacagat tggccattat agcttccttt aatcccaaat agattgtaat
    19621 taggggcaga agccaaagta ctgttccccc agccactttc aacgatggct tgagcaatca
    19681 tgactgatgc atataagtca ttggcacttg ccaaactttg tgcagttgag gcaatactat
    19741 caatgaacgc ttgttgagaa ttagcagatg agcgagcata aaaagcatag ccagtatagt
    19801 tttcgaattc agacaaatct gcttgatggg tatgtgtcaa tgtttgccca tctgtcaatt
    19861 ggttcgtgat ttcttctgca ttctctggat tgttctgagc ttctgctgat gtactgtctt
    19921 cggtttgtgt ttgttctgtt gtttcagttg ttggttcttc ggttgaaatt gtattttctg
    19981 agacagtagc tgactgcaaa gtactctctg tatcgttttc aattcctgtt tctgaagaac
    20041 tcgatgtctg agttgcgaca gtttctgtcg attcttcagc tagcactgtt gtgttggcaa
    20101 ctgctagatt tcctaaaaga ataacgctaa ataataaatt ctttgctttc atcttttttc
    20161 tcctttgaat tatgaaactt ttcctaaata acagtgtaac taaaaaagta aaagcaagtc
    20221 aatgtccata tttaaataac atttgtttct ttttgataaa tttgcttttt ctacataaaa
    20281 agacgtaaat gaagaatatt cttctccatt cacgccttaa attgatggtc gtttgaataa
    20341 ctatttaaca agtaattctc tttggatatt atccgttatg gattatactt cctgtgtcgt
    20401 cggacggata atcatgttac tgacagacat ccggtctggc gtttctatcg caaatgctac
    20461 agcagaagct atatcttcgg aggtcaatcc ccattctttt tgcgcttcat gaagttcaag
    20521 agcagccttt ttgtcgctga tggtttgata taattcagtt tgaacagctc ctggagagat
    20581 gatcgtagat ttgatgttat tttgccgttg ctcttgacgt aatccttcca tgattgcacg
    20641 gacagcaaat tttgttccgc aataaacggc ggaatcagga taaacaacat gtcccgccac
    20701 agaatcagtt gaaatgatat gcccagattt ttgtttttcc atgattggaa gtacggcgga
    20761 aattccattt aacacaccca tgatgttgat atcaagcata ttttgccatt catcacggtg
    20821 accttcaact aatggagcgg ttggcatgat tcctgcatta ttgtacaata catcgattcg
    20881 accgaacttt tccatcgcta aatcgataac tgcttggact tgtgcaaaat ctcttacatc
    20941 tgctgtttga atatatagct cagcttctgg taaagattct tttattgctt ttaggcgatc
    21001 ttctcggcgt gcagctataa ctagtttagc ccctttttct gctaatagct tcgttgttgc
    21061 ttcaccgatt ccgcttgaag cgcccatgat gacaatcact ttttctgata aatcattcat
    21121 tctattctcc tcctgttttg aaaaatctct tgcttatact cactatctct tatttcatct
    21181 tactgttcaa ataatccgtt cgacaggttt gacgattttg gcagggattc ctgcaacgat
    21241 ggtattgtct gggacatctt tcgtaacagt tgcattcgct gcaacaatgg cattctcgcc
    21301 aatcgttacg cctggcagaa tagttgctcc tgctccgatc caagcatttt tcttgatgtg
    21361 gattggcttt actgacaagc ctcttcgttc ttttggatcg gttagatggt tcaccgtgat
    21421 gaggttgaca cgaggaccaa tcaatactga atcttctaaa cagatgccac ctaagtcaac
    21481 gaacatcact tgacgattga taaagacatt tttccctata ctaatatgtt tgccgaaatc
    21541 tgtataaaaa ggcagcagaa cagtcaccgt atcatctact ttgtcactaa tgatattttc
    21601 tagccgtttg cggatatctg ctggttcatg gtacccagta ttcagcgctg ctaaaggctc
    21661 ttcgttttca gcttggatct gatggatcac ttcaaagagc ggatccgtta ctcgaatctc
    21721 tttattgacg atttgttttt cgatgaggct tgtcattttg ctccctcctt ttatccttac
    21781 tttataacgg acggcttatc atgtctaata cttataaaac atacttaact attcctttta
    21841 actatactta gtggtatgct tttttaaaaa caggagggaa aaaatggata ttcgtatact
    21901 gacgtatttt ttagcagtag ctagagaaaa aaatatcagc cgagcagcaa aatctctgca
    21961 tatcacgcaa cctactctct caaaacaact gaaagattta gaagaagaac ttggtgtgac
    22021 acttttcact cggggctccc gagagataca gttgacggat tctggtcgat atctttattc
    22081 aaaaggaaaa gaaatcttgt cgttggtaga aaaaacggcg aacaatttaa caaatgacgc
    22141 aatcatctct ggagaaatct acattggcgg aggcgaaact aaagcaatct ctttcatttc
    22201 tgatagcctt cattcgttga tcaaggcgca tcctgatatc cagtttcatt tatatagcgg
    22261 gaatgcagat gatatcatag acaaactgga tagcggatta cttgattttg gactcgtaat
    22321 cgaaccgaca aacaaacaaa aataccagtc atttcgactg ccagcccaag atcgttgggg
    22381 acttttgata catcgaaatc atcctttagc aaagcaaacc gtcatccatc cgcaggaact
    22441 gaaaactgtt cctttgatca tttcccggca aacgtcagtg gacagtcaac tagctgaatg
    22501 gctaggtttc agcatagatc agttatcgat cgtcggaaca tataacttat tgtacaatgc
    22561 ttctttgatg gtcgataacg ggacttttgg tgcattagct ctagatggga tcatcaatac
    22621 ttatgggacg gaaaaaatat tcatcccact cgatccgcca ttacaagctt cactgaatct
    22681 catttggaaa aaagaccagc ctctttctcc tgctgctcgt aaatttttag atgtcctaac
    22741 ggagaaatac agttaagtag agtttttttc cttcgctttc agctttttaa caacgaataa
    22801 gataagcaaa agatccaacg attaaataac ataagtttct taatttattt cttcctttta
    22861 tttctgataa ttccagtaaa ttactataat tataaaaaaa gaattgacaa aacataactg
    22921 tctatataaa attgattacg ttaacaataa ttcgtttcat gtgactagat catactaggc
    22981 atgaaaaatc tctgaggaac atggtttccc taggaggttt ttcgtgtcta tttttgtttg
    23041 gccgcaatca actggtctag caaacgtgaa aaccaaatct tcaaaaaggg tgcgcgcatg
    23101 aaaatcaaaa agatctttaa tcaaaatgca gtattggtag atgacggtgg acaggaaaaa
    23161 gtagctattg gaaaagggat tggatttgac agaaaaagaa atgatttgat ttttgatcgt
    23221 gatgtcgaac ggatatttgt catggaacca gaaggtcaaa tcaagttgca aaatttactt
    23281 aatcagattg atgaacgctt tttatttgca gcagaaacga tcattgatca tgcggaaact
    23341 gttttgatgg aaaagttgaa tgaacatgta ttgatcgctt tgaccgacca cattgctttt
    23401 tctgcggaga acattaataa cggtatcgtc atccgcaaca aacttttaag agaaatcgaa
    23461 gtgctttaca gtgatgaatt cagtattgct caatgggcag tagagtatct gacaaaagag
    23521 cttgggattc catatactta tgacgaagca ggatatatcg caatccatat tcacagcgga
    23581 cgaagcggtc ggaacaataa ccatcgcagt atccgtgaag tgacgattgt atctgacatc
    23641 atccatttag tggaatcaga attggaaaca gatatccatt cagaaccatt ttcactgaat
    23701 tattcaagac tggtgaatca tttacgactg cttctacaac gaacccatgc gcaacaatat
    23761 gctgttttag acacggaaat cgtagaaatg gtcaaacgaa aatacccaga aagctataag
    23821 atttctaaaa aaatccgtgt gttgctgacc aaagaatacc agctcgcgat cacgaaagaa
    23881 gaactgggtt acttggccat ccatatcgag cgtttaagaa gcgcaatcgt acaaacaaat
    23941 gttaacaatc atgaggagga aaaaaattga aagcatatct acaacgaatg ggacgttccc
    24001 ttcaacttcc agttgcggtc ttacctgctg ctgctttact agtagggatc ggaaactggt
    24061 gggcatctta tagtaacgac attgtcgctc attttttaca agctggcggt aacgcagttt
    24121 taggtcaatt acctttgctg tttgccgttg ggttagcact agggatgtca gtggacaaat
    24181 caggtgctgc tgccttggca ggtttggtcg catttgaagt tccagtaaat gttttaaaaa
    24241 ctgattcagt agctacttta ttgaatatca aaccagaatc agttgatcca tcttttgctc
    24301 agatcagtaa cgtatttatt ggtattattg ccggattgat tgcggcagct ctttataatc
    24361 gtttccacga agcaaaatta ccgatggctt tatctttctt tagcggcaag cgtctagtgc
    24421 caatcttggc tgcctttgtc atgttgctga tcagtgcagt gctactgctc gtatggcctc
    24481 cggtttactc tgctttagtt tcttttgcta aattcatttt gagtttaggc tggggtggtg
    24541 caggtcttta tggcttcttc aaccgtttac tgatccctac aggattgcat caagcaatga
    24601 actccgtgtt ctggtttgac gtagcaggaa tcaatgatat tggtaaattt ttggcttctg
    24661 aaggaacaaa aggcgtaaca ggaatgtatc aagctggatt tttcccaatc atgatgttcg
    24721 ggcttccagc aggagcatat gctatttaca gaaatgcacg tccagaaaac aaagcaaaaa
    24781 cagcttcctt gatgctagca gcagcttttg cttcattctt tactggtgtg acagaaccac
    24841 ttgaattttc atttatgttc gttgcttggc cattatatgt cttgcatgca attttcactg
    24901 ggatttcttt agcagtgagt gcattcttcc attggacagc aggatttgcc tttagtgccg
    24961 gatttgtcga ttacttccta tctttgaaaa atccgattgc gaatcagcca ttgatgttga
    25021 tcgtccaagg attagtaaca gcagtgattt actactttgg ctttaacttt gctatcaaaa
    25081 aattccatct gatgactcct ggccgcgagg aagcagatct ttcagatgac gatactgcaa
    25141 cgactaattc agacaataaa tatgcggcac aagcaagcaa aatctacgct gcattaggcg
    25201 gagccgacaa tgtaacttcg atcgataact gtactacacg ccttcgtttg caagtcaaag
    25261 atacaggcct gatcgatcaa aataaaatca aagccactgg cgtacctggt atgaagatca
    25321 tcgacggaaa aaatgcgcaa gtcatcgtag gtacagaagt acaattcgtt gcagatgaga
    25381 tggcaaaact acatggtggt gctgcagcac gacctgcaca aacaaacacc gtggttaaaa
    25441 cagaaacatt tgccggtgaa acgatcgaac aagatattta cgctatcgca aatgggaaac
    25501 tgattccaat cactgaagtt tcagatgatg tattcgcgga aaaaatgatg ggtgacggct
    25561 atgctgtatt accagaaaat ggagaaatct tctctccaat cgctggtacg atcacaaaca
    25621 ttttcccaac gaaacatgct gtcggaatcc aaacagatgc tggtatcgaa gtcttgcttc
    25681 atatgggcat caacactgtt gacttaaaag gcgaaccatt cactttatat gtagaagaag
    25741 gtcaaaaagt cgcaagaggt caattgattg ctttagttga tcttgccgct attcaatctg
    25801 caggcaaaaa cacggatatg gtcgttgtat tcacaaatgg cgacaaagtc caaagcttag
    25861 aaatcgaacc cgcacgtgat gtaaaagcaa atgataaaat cggctctgtt tccaacaaag
    25921 cctgagccat cgtcttcccc agactttttt atcgataaac aaacaggagc tgagacaaaa
    25981 atcttgtctc agctcctttt tccgaataaa aacggcgttc tcctgccaac tccgcggtaa
    26041 taagtccagc taaacgaaac atatgaagag ctatttttta tttgactctt cttattactt
    26101 gatgcagaac agctttttca caaaattttt cttcatactc aaaactaatc ggctttttca
    26161 caacctcttt atacattatt cctgatccaa tatttctttt aatgctgctt caagagaagt
    26221 taatggtttt cccaatactt tttctaaatc atcagaggaa atagctaact gatccttttt
    26281 aatatcatgt tggatcgcta aaaacatgtc agctgcttct tgagggaatc cggcttcttt
    26341 tagattttcg acaaacgctg catcacttgc ttcaataatt tctaactctt tacctgtcgc
    26401 acgctctaat gccttggcta attcttcata gtcagttaga ttgccggata attctaagat
    26461 ttctggagag tcactttcta ctagtacttt tgctgcagct tctgcatatt cacgtttcaa
    26521 tgcccaacct gtttttccat ttcctgcagc ataaacgaat tgaccagttt ttaaagcctg
    26581 cccaatgata ggtaattcat tctctacata ccaattattc cgtaaaaatg tatgggaaat
    26641 acctgaatcc ttaatcattt tttcagtgaa ttggtgatct ggcgctaata cactgatcga
    26701 gtgatcggca tctgcaaaac ttgtataagc gatataagat accccagctg cttgtgctgc
    26761 cttcactaca ttcttatgtt cggcttgacg atttcctgga acgccagaga caaaaagcaa
    26821 tcgatctatt cctttcaacg cttctttcat tgagtctaaa tcatcataat caccaatacg
    26881 tatattaaat ccagcttctt tcagtgcttc tcctttttct tcactacgta ctagtacata
    26941 gatttcattt tgtggaacca tcttttttaa gaattccagc gcataaccac caaaacctcc
    27001 agttgctcct gttactaaat aatgcatatt tgccactccc tattcttttt atttgttaag
    27061 atttacagct gtacatttat aaattacacc tttatcataa agagtgtggt aagataagtc
    27121 aagcaaaaaa aatgaaagga tgacaactga tgaagtattc tctgcaattt agtgatgcaa
    27181 tccatgtact tgcctatatt gaaatgtttc aagatacccg atcactttct agtgagatga
    27241 ttgccagtag tatcgaaaca aatgcagcaa atgtacgtaa aatcatgagt cagctcaaaa
    27301 aatccggttt gatctttaca aaaaccggaa aaccgcagcc ttcccttgcc aaaagtccag
    27361 atcagatttc tttactagat gtctatcgca gcatcgaagg taatacacag cttatccaag
    27421 tcgatccaaa aacaaatcca aattgcgtcg ttggtgcgaa tatccaagaa gctttagaag
    27481 aaagctacgc tcggctgcag aaaaaggcag aagaagaaat ggctaagatc acacttgata
    27541 gtctgataca taaaatagcc gttttggaaa aagaaaaaag accagaaaac acaggcctcg
    27601 tagagagatt tttataattc tctctactgt gcctgcattt ttctgatcgg aatcgtctac
    27661 ataaattctt ttattctccg ggggacaaaa gccttgtcat gccccttttt cctctctaat
    27721 gctcaggtca aagcgctgaa gtcatgacgt cttgataaac ggtgttcaag tagctgaccc
    27781 tcggcattag ttctgaaaaa tgcaaaatat ggaaagctat ttcccatttt tcctctctaa
    27841 tgctcaggtc aaagcgctac tttcacgacc tctatattat catggtttat tgactaccgg
    27901 tatccagatt tctcccatat atttcccgtt ggtaactccc atttcgacta cagcatttgg
    27961 acccccaaca taggcgtaat cttctacttc tcttaaaaca tgcccaaagg ccgtattcgt
    28021 caaatcattt cttaattctt caggcgattc tgctaaagcg cttacaacaa gatacgttcc
    28081 ttttgggaat gggatgattc gcgtagtgtt tggaacatcc tgactcgttt gaacaccgac
    28141 atagtacatc attttcccgt tatagggttc gttgactgta aagaaataat gattttcagc
    28201 agcttgtttc aatgcatcta ttgttccatt accgataact ttttgctcaa atgcttgttt
    28261 ctctgcagcc aaaccgacaa aatccgtatt gctactcttc aattccacac caatacccgt
    28321 caccgtaaaa tctggttttt cttcgatcct atactttgcc atttctccca ccctttctat
    28381 tatattaagt tcatcttaca taaaattaaa gggaaatgac acgattacgc tcttttttct
    28441 agggatattt ttatttttta actatttttt tgctttcact gcaatccaaa tctcactgta
    28501 aacattactg aaatcctctc cccattttgt aaaagagatc tcgggagctt cgactaaccc
    28561 ataatctgaa gaaggaagcc attctgcata gattttcccc caagtatctt gtaaagtttg
    28621 gggaaatggc cctttattag gaaaaatggc ccaagtgtgt tcttctatag caagcatttc
    28681 taaatcagcg tacggatttt cttttgtcgt agcaaagcca atcatgtgca tcaaactgcc
    28741 cttttcttcc aagcggtctc catcaaatcc ataagaaacg ttgattactt gatttggata
    28801 taaatctcct aacttatgca tctcttccct ttgagtctcc gtgatcgtct gcgctaactt
    28861 ttgtatttct tgattgactc cttcaaattt gacaggaact tttttcgcca cacctgccac
    28921 ataaaactgt tctttctttt cgatacgaaa ttccatcgat tttcctcctc tgacatcaat
    28981 aaaaaatgtc aagcgtggaa aagatttttg tatgccgtat ctactgattt ctgaaggcag
    29041 atatcctgac caatcacgaa aagctcttga aaaaccttcg actgtttcat atccatatct
    29101 aaacgccgtt tctgtcactc ttgatccatc tagaagatcg cgattagcca aagccaatct
    29161 acggtttttg atatagtcca ttagcgacat ccctgcaata aacgtaaaga tgcgttttaa
    29221 ttgatattca gagattcctg tattttcggc tattatttta taggaaagtc cttctgtcaa
    29281 atgttcttcg atgtaatcga tcaattggtt cagatcttta agcatattta tcttcctcct
    29341 tgattgcctt tttatcatat tatcaaagaa agtcaattac ttgatgaatc aggaaccaat
    29401 ttagtcagat gtagaaaatg ttgtatggat ctaaagaaaa ttctcaagca gcgtttcgta
    29461 acggataatt ggtattatct gcctatctat gttttacctt tagaaaaata aattttgggg
    29521 aagacagatt ctaagtaaac aaagcggtaa aaatatcttt tattccctta gttaatccgc
    29581 ttactaccta ctgtacaaag caaattgttg ttcaaactgc tgatcagaac gctgcttgga
    29641 tttttctttg cgatacatca tacctctttt atcaattgga acaattattt atttctaaca
    29701 aaatcacttc atttttgtca gataaataaa aaacaaaaaa agttccatct ttaaaacacg
    29761 ctattctttt actttttctc acattctcta agaaaagaaa aggagaatga atttttgaca
    29821 attcgagcaa aagtgattat caaatatgta aaatcagcta cttttttctt aaacgtttct
    29881 ttatactaaa tataaataag tgtttcatga aaggagaact agatgaataa ttggcttgag
    29941 tattttcctg aaaacgtttt ggaacgcggt tacagctatc atctgcacgg ttttgtccgt
    30001 catttgaact atacaagcaa gtacttgtcc gcaacagttt ctggcacgga agattataag
    30061 gtagtgatca cttgggatga aaagaccaac atgacttgtg attgtctata tgccatagaa
    30121 ggaaaaaaat gcaaacacat ggctgctgtc ttattcgctt acgaagaacg tccaataaaa
    30181 aaatccaact actcactttc cgaactctcg tcccttgttt cctccgctag ttcctctctt
    30241 gtacgggagc ttttaacaga gattcttata gaacatccgc actttatcga acgcttcaaa
    30301 gttaagatgc catttcatgc aataaattat tcagataaat taacaactat catccataaa
    30361 tacgatcata ttatcaagaa aaacaaaaac aggaaaacag cgaagttcat catggagatg
    30421 cgaaagttca tccaagaagc agttgaatcg cttattcagc aaaatgccta tcttcccgca
    30481 ttcgaattga tcaatgaagt gattgctact ctcgaaacat tctattggga gcctgaggat
    30541 gaacgaaccc ttcttctgat agaggactgt tattatttat ggaaagaatt attagcagaa
    30601 gcacctcatg cagaaaaaag gcaaatgttc tcttggttcg tctgccaagt cgatcatact
    30661 gacgcctctt attcaaaacg ctactctata aaaatcctga aagaagactt tcgggaaaaa
    30721 gaattttcta accaaaagaa aaaaatagat aaaaaactaa agaaacggta aagaaagacg
    30781 atttcaatga aaaatagcaa gtaaacgata acaaccatcc taaatccaca agaaatacag
    30841 cagaccttgc tttaaaaaca cgaatcatgc aaaggatgaa gtaaagaaga cttaacgcgt
    30901 acccaatatt agtcatttga ctattaggta cgcgttttgt tttataagtc atgacaaatt
    30961 tccttcgttg ctggatagat tttccggcat tgttgataga ttttatgata cttttctaca
    31021 ttttgtaaga gaggttccgc tgcatctgta tattggataa aattctgaca acaactttca
    31081 ggtgtgtcga accaccctaa ccctagtgca gcaatcattg ttgcgcctaa tgcaggtcct
    31141 tcctccatac ttaaacaata gaccgttgca tcgaaaatat cagcttggat ttgtagccat
    31201 gcaagatttt tcgcgcctcc tcccaccgaa acaatacgtg taaatctgcg acccgctagt
    31261 tctttacgtt tcaatcccac cattgacaaa tgctcattaa aaatttagaa tattcacaag
    31321 tttaacgaca attatgaaaa tttatgtcgt gctaaaggag ttttgttatt ggatgaaaag
    31381 ttagatataa aaactgcgat caaagacacg ttacctaccg ttttcggtta tatcggtatt
    31441 ggacttgcat ttggtatcgt tgggaaagct gccggatttc atccactagt cgtcacgttg
    31501 atgtccctgc tggtctatgc tggttctgcc caatttatca cagtcagcat gcttgctagt
    31561 cacagcccat tgctttccat cgttttctcg acctttctag tcaattcccg aatgattctg
    31621 atgagtatga cgattgctcc ttatttcaag aaaaatcggc tgcttcaaaa tctgctgatt
    31681 ggcacattgc tgacagatga aagtttcgca ctgggaatga ataaattgaa ctatactgga
    31741 caaaagttaa actttcgttg gatgaataca gctaattgga tttcttattt gacgtgggtg
    31801 ggttcttctc ttgttggcgc acttttaggg aattttatta ctgatccgaa aaagtttggt
    31861 ttggatttcg ccatcgtcgc tatgtttatc gggttacttt atcttcaagt catttctgat
    31921 agaaacacct ctaaacgatt acagttgatc ctgattggtc tcactttgat tctggtatat
    31981 gtcggactga tttttatccc aagcaactta gtgattgtag ttgtaacact gattgggtgc
    32041 ggattggggg tgtggatcaa acatgccttc ttttgagttt acattgctga ctattttagg
    32101 ttgtacttta gcaacttggc tttctagggt cctaccattt gtcttactga aaaaattcga
    32161 tttaccgcaa ccgttgcttg agtatttaag ttttgtcccg atcgtcatta tgtctgcttt
    32221 atggttcagc agtctattta cccaaaatat cggacatctg ccgcagatca atctcgaaaa
    32281 tgctttggct tcagttccta cacttttagc tgctattctt tcaaaaagct tattggtgat
    32341 cgtcttggca ggaattcttt ccctttcgtt gattcgctta ttataaataa aaaaacaaga
    32401 gcttatggca aattactgtc atgcgctctt gtttttatca aagtcaaata agaaaaaaat
    32461 cggcgtattc ttgcttatcc gttgaacgga ccactaacaa cgacttttcc taacatatgt
    32521 cctgtttcta cgtctttggt tgcttgtttc aagttcttcg cattcatccc atctgaatag
    32581 accttgctca atgtactgcg gataatccct tcatttgcta aagcagcaat gtgcgctaaa
    32641 gcttctcctt gcgtttcgat ttcatagtta tagtctgttt tagcgaacat atactcccag
    32701 tcgaagctga tagattggtt tttccaaaca cctatatcta acggctcttt gatccctacg
    32761 atcgttccca catgtccaaa tggtcggaca aggtgcttga tttgatcaaa gtagcgggta
    32821 atatcgaata atacagcgac atactctact tgatcgattc ctaattcacg aaaactttta
    32881 cttaagtcgt gatgataatc gattggatag tctacacctg tttttttcag ccattcaaaa
    32941 ttcttcggac ttgcagtcgc gtagacttct aggccagccc aatgcgctag ttgattcaaa
    33001 atcgaaccga cgcccccgga tccgttgatc actaagattt ttttcccgcg attggcattt
    33061 tcttctggag ttagaccaaa tttttcaaat aacaattcat aagcggtcaa tgaggtcaac
    33121 ggcaaggctg cagcttcttc atctgaaaga ttttttggtg caagagcgac aatacgttcg
    33181 tcaactagtt ggtattcttg attgcttcct gctcttgttg ttgtacctgc gtaaaaaacg
    33241 cgatcgccga cagaaaattt ttgcacttcg tcacctacag caacaacttt cccgactcca
    33301 tcaaaaccta agatacgtaa agcatttctt atcccgttat cttggcgaag tttcgtatcg
    33361 acaggattaa cagatactgc gttgactttt accagaagat cccgtgaact gggtacaggt
    33421 atctgttctt tttcatcaat aaagcttttt ggatcatcga tcggcaaccc ttcataaaaa
    33481 ccgactacct tcattaattc cttttctgac atacgaattc ctccttagct tcatcaagtt
    33541 ggagcatcca tacagtgttc atttcaatat atatagtatg ctatttgttc cacctttttt
    33601 attcatcata attattctct tacttaaagt aaactatttg cacttttttg gttaaaaaag
    33661 aggttgcgac aagacttttg tcacaacctc ttataaacgg tgttctccta ccaactcttc
    33721 ggtcacaagt caaaatattc atcaaacatg agaagctgtt ttttgaaatt gctccttgtg
    33781 actcagagtt aaacggcagg ctcacaacct ctgatataaa cggtgttcaa tcaactgacc
    33841 ctcggtatta gctcaaaaaa cgacaaaata tgaagagcta tttttgtttg gctgttctta
    33901 ttacttgata caggacagct ttttcacgac ctctaattca cggtgttcaa gcaagaaatc
    33961 attgttcact gaaaagcgtt ggatgttctt tttcaaaggt ctctaagatc tctcgtactt
    34021 catcagtaga tttcgtattc atcaactgaa ctcgcaagtc atttgctcct ggaaatcctt
    34081 tgatatagat tttaaagaaa cgatgtagtc ctgtgatgga acgcgggatg atttctgaat
    34141 aatgatcttg cagatctagt tgcagacgaa gtagtccgat cagttctttt tctgtatgtt
    34201 ccctcgggtc tttttcaaat gcataaggat tcttgaaaat ccctcggcca atcatgatgc
    34261 catcaacgcc atatttctcg gccaattctt ctcctgtctg gcgatcgggg atatctccat
    34321 tgatcgtgat tagtgtttgt ggagcgattt ggtcacgtaa agcaatgatt ttaggaatga
    34381 cttcccaatg tgcatctact ttactcatct ctttacgcgt acgcaaatgg acagaaagat
    34441 tggctatatc ttgatgaagt aggtgactga tccattcttc catctcagac atttccgtat
    34501 agccgatacg cgtcttgacg ctgacaggca aaccgccagc tttagctgct tcaatcaatt
    34561 gtgccgctac ttctggacga aggatcaagc cactgccttt tccacgttct gctacattag
    34621 ggactgggca gcccatgttg atatcgattc cttggaagcc catttctgcc atagcaatgc
    34681 tcatttctct aaaaaattct ggcttgtctc cccaaatatg ggcaaccatc ggctgttcat
    34741 cttctgtaaa tgttaatctt cctcgtacac tgtcttttcc atctggatga cagaaactgt
    34801 ctgagtttgt aaactcagta aagaagacat ccggtgctgc tgcatgcttt acgacatggc
    34861 gaaatacaac atctgttaca tcttccattg gtgctaaaac aaaaaaaggc tttggcagct
    34921 ctttccaaaa attagtagtc atttatttgt tactctccat tctatcaagc agtgaagtcg
    34981 caatctttct caaaaacatt gtcattatac taggaaacaa aaaaagaagc aaagtgaaca
    35041 gcttaaaaga tgtttctgat tgagcaaaaa aaagactgtg acaaaaatct tatcacagtc
    35101 cattttccgg aataaatggt gttcaaaagc ttgcatttgc ggtaataagt ccaaccaaac
    35161 gaaaaatagg aggagctatt tttgtttggc tgttcttatt acttgatgca gaacagcttt
    35221 ttcacaacct ctccactaaa cggtgttcaa tcagctgacc ctcggtattc gctcaaaaaa
    35281 caacaaaata tgaggaacta ttttcccgtt tgtttctcta atactcaggt caaaacgctg
    35341 atttcacaac ctcttttcta acttctcaat cctcttcctt tattgatcaa ataccaagta
    35401 acggcgtaca aaacaacgat gaataacaca agtacgatca tcgatgttgc tactggcaca
    35461 tcgatcgttc caaggaatcc gtaacggaaa ccggagatca tataaacgat cggattgact
    35521 tttgaaacag cttgccaaaa gggcggtaac atagagatgg catagaaaac gccacctaga
    35581 taagttaacg gctgcaagac aaatgtaggc acgatcgata catcatcata agactgagca
    35641 aaaatcccgt tgagcagtcc tgctaatgaa aacaagattg ccgtcatcaa taatgtaatg
    35701 atgaccatga accatgaata cacatgaagt ggtacaaaga acaaggaaat gatcgttacg
    35761 agagagccga cgagaatact gcgacccaca ccaccaatca caaagcccca aataatcaca
    35821 tgcgtaggaa caggtgctac caagatttct tcgatatttt tctgaaattt ttgagagaag
    35881 aaggacgaag aaacattggc atacgaacta gtgatagcag acatcataat caaaccagga
    35941 acgatgaatt ccatatatga aaaaccaccc atgtcaccga tccgcccacc gatcattttt
    36001 ccgaaaataa caaaatacaa ggatgtcgtg atcactggcg gaaccagtgt ttggacccat
    36061 atacgcaaat agcggttcgt ttccttgata gctaaacttt ttaatgcagt aaaataaaga
    36121 ttaaacattt ttctcctccg tatgattatt ttcttctgtg attttcaaga acagttcttc
    36181 taaacgattc gacttattcc tcatagaaag aactttgatt ccttgtcttg taagctgatc
    36241 gaagattccg ttcacccctt gatttcgttc aacttcgaca gcgattgtct gctcatcttc
    36301 aaatgtacaa gcatatcctt caatcacagg tttggtatca tatggcgcta aatcaaagat
    36361 aaatgtttca aattgcagtt ttgacagaag ctctttcata cttgtattct cgatcagttc
    36421 acccgattgg atgattccga tgttgcgaca aagcatttca gcttcttcta gataatgcgt
    36481 cgtcaaaatg atcgttgtac cattttcgtt caattctcgt agaaactccc acatctcgcg
    36541 tctcaactcg atgtcaactc ctgctgttgg ttcatccaag atcagcaaac gtggttcatg
    36601 catcaatgcc ctagcaatca tcagtcttcg cttcattccc ccggaaagca tacgtgcacg
    36661 gacatttctc ttttcccaaa gattggactg cttcaaatat ttttcgctgc gtttcatcgc
    36721 ttctttgcga gagacaccgt agtagcctgc ttgattcacg acgatttgct ggaccgtttc
    36781 aaaagggtta aaattgaact cttgtggaac caatccaatc tgttgctttg ctcgaactag
    36841 atccttgtcc agatcataac cgaagacatt cacttttcca gatgttttat tgactaaaga
    36901 agtaataatc ccgatcgttg tagacttccc agctccattt ggccctaata gcgcatagaa
    36961 atctccttct ttcaccgaaa gatcgattcc ccgcaatgct tcgaccccac ttccatagat
    37021 ctttttcaaa ttatttattt caagcgcatt tgccatttat ttcctatctc cttatagaca
    37081 tgattcttaa ttgtatcatg gaatacagca gatgaaaaaa caacttggct tccacacaaa
    37141 aacacacctg ctaacaaaga ggagatgtgt tttctagttc atgatcatct tcgatagaat
    37201 aactatctaa acaactctgg aacttttaaa atcataaaag taccgacagc aactactacg
    37261 atgacatata gaagatcctt ataactgagg ttcttgtctt ttttattcat gttaagcttt
    37321 cctccgtcct tttatcgaaa gcttttttct tttattttaa ccgaaataaa taaaatggaa
    37381 atagttattt tcaatttctg ttttcgctgt ttgttttacg gagaactttg ccatggaacg
    37441 tgcgtctcgt cgctgatgct aagttttttc gataaaagct gctagaagga agacagcgtg
    37501 gtttattttg tatcgtcttt tgctaaatct tccaagatca tctggacagc agactcgttc
    37561 ccattgaatt ctgccacttt tttggcatca atgtacttct tttgataatc gtctttttgg
    37621 acccacgcat catgcttcgt ttgataaagt taatcaaacg gataccactg acgctgttca
    37681 tcgaacaaca aactttcttt ttgggcctgt tcaactatgg atttaggata attcaaaact
    37741 ttcagcaagg caatcgcatt tcgggtcctt gaaggtcctt cttttaattt gaaatcaaaa
    37801 ctgattcctt ttccttctgt tacttgttct tcaaagtgga cattctcaca ataattcttt
    37861 aaaatctctg tcaattcgat atcatgtgtg gcgacaaatg ctaaagatgg atactccgca
    37921 agccaccgaa cgactgaagc ggaagaagca atccgttcga tcgtatttgt tccttttaaa
    37981 atctcatcta cgaagcagta gcagcgttct ttggaagcca cttggtccag caaacgtttg
    38041 atcgatttga tttctgaaac aaaataactg tcgccttcaa ataaatcatc ttcgattgcc
    38101 atggaagtca acacatgtcc aggttgcagg gtaaactgtt ctgcaatcgc tgtttggatc
    38161 gtctgtgcta gaatacagct gatggctata cttttgacat acgtagattt tcctgatgca
    38221 ttggaacccg tgactaatgt attcttctgc cagttgactg gattaactac cgcttctttt
    38281 aacaatggat gatagatatc tttcgctgtc acaccgccct cagtaaactc tggtcggcaa
    38341 gtgatcggca tataggtgcg ataattcaaa acggctgctg caacttccaa ttctcccagc
    38401 aattcccaca gcttgatggc ttcagttgaa tgcttttcta atttagtgag aacgaaatga
    38461 taggagataa atggcagcat gaacatgatg ttgacatatt caaaaatcaa ttctgcttca
    38521 ctcccgctct tcattcgaaa tgaaaaacct aatttaggga tgtctcttaa tggtttagtt
    38581 tgctgtttca gctctttttg aagtggtgtc gtcatttttg ctaactgatt ggcggctgaa
    38641 atcgttgaaa ccaaataact catgctattc agctctgttt ctagtgtttg tttcttcact
    38701 agataataga ccgtattgaa tactaagcca ccgattgcta gatagattcc tagtacttgc
    38761 cctaaaagaa gcaaacctac acccaccagc ggaagcaagc caagaaaaat aaataaacct
    38821 acgcgtccaa tctttttact ttttccatcc gctagatatt gttttgcaaa attatgatct
    38881 ttttttccta aacgagcgaa ttgatattgg attctttctc tcagttgagg attttcctga
    38941 taaaaagcaa ttagtttttc taactgataa tcttctccaa aatcaaagct acgtaaacgc
    39001 tgatacaacg cttctgaacc tacactagaa taggtactgt tgatcccttc aaatacttcg
    39061 aacatatcca agtcatacca agtaagatcg tcgatttcac tatcccatga acggaatttt
    39121 ttctccgtga gccacgcatc cttcaaactt tcttctttat caaatctcgg ttgataagga
    39181 atttttcccc aattactacg aactgtttcc tttactttca aacgtgaaaa aatgtctaaa
    39241 acaactaata aaataacaac tgcaataata attaaaacga taaattctct ttggcccata
    39301 tccctctccg ttccttgatt tgttagtttt agtatagcac tgttgcgcta tttgaaaagg
    39361 gggtacaaaa ataactgtct tcgttaccta attcgtatca atcacaccaa aataaaaaca
    39421 ccttccaaaa aagaagatgt ctttaaaacg agatacaatt ttttgtcatt cacttcaggt
    39481 aaattagagc caattaaacc ctccgcaact tgacggagat ggagaagatg cccccgtcta
    39541 atctagcggt catctctcct cccattcctt ccaccaattt ttttgacagg taaagcccga
    39601 ttccgctaga ttccgtattt gtgcggctgg tattttcggt ataaaaacgc tggaagactt
    39661 tttcaacaga ggtttgcgct cctggtttga taggattggc cactgttaat cgaatgaatg
    39721 aatcctcttt tgaaaatgtc acctttgctt gtccatctgc ataacgcaat aaattctgta
    39781 tgatattttg cagcactcgc agcaacatga gcggatctgc taatacagtt gtatttatcg
    39841 agtgttcagc gaacgctacc tcgatatttt tctcagaaaa ttgttcataa aaagagaaaa
    39901 atacttcttc cacctcttta gaaaaagaaa gcgtatggag atccatttct ttttgcttcg
    39961 tctctattcg agcaagatca taaaactgat ccgtcatact gagtaatctt gcaacagatt
    40021 gtttgatgat cgttaaatac ttctcccgtt tctccgtatc ctctacctgt tccagtaatt
    40081 gtacataacc gttaatagca gtcaaaggag ttctgagatc atgagaaagg ccgacgatcg
    40141 atagtctgat gttttcttcc atttcttgaa atgcttgtcc tttttgttca ttttcatcca
    40201 cccatgtatt cagtgcatct actaggtcca ttagattctt ttctcgaaat tcgactgaaa
    40261 gacgaccacc atattcttgt cgttcagaaa gagtggcgat tttcttagtt aatctcttga
    40321 tttccctttg caggaagaaa ataaaacttc ccataaagat aacaacgaga agcaacagcc
    40381 acttaatcaa atctaaaccc cactccccac accgtatgaa tatacacagc atgtgccttt
    40441 gctagtttcc ctcgaagatt ggaaatatgc atattcactg ttttctcact atcaaaatat
    40501 ggttcttgcc aaactgtttc ataaagattt gctcgcgaaa atactttttt cggatgttct
    40561 aaaaaaagct tcagcagatc gaattctctt cctgtcaaat ggacagccac accattcaca
    40621 tttacatccc tcgactcttt gtccagtaag atttccttgt aatagatgcg ttgcgacaga
    40681 tcttttactt ggacagaagt ctgatgacga agttggatgc cgattcgtaa caacagttct
    40741 tcgatatcaa atggtttcgt cacatagtca tccgctcctg ctgccaacaa ggaaacttta
    40801 tctctttgat cgctttttgc tgaaagaacg atgatcggaa cattcgatgt ctgtcggatt
    40861 tctgctaaca atgcttcacc tgaaagccct ggaagcatga gatctagaag gatcaaatcg
    40921 acaggatatg tgcttaacaa taattttcct tccgtacctg aataagcatc aagaagtttg
    40981 tatctctgtt caagaacctc ctccagtaaa gaatgaacag cttcatcgtc ttcgataatc
    41041 aaaatagttt ccagcaggaa cacctactta atgtcagatt tttggaaaat aaacattcct
    41101 agagctgatg ataccacgat tgttactaga ctgaccagaa tcattttcgg tgcttgcgaa
    41161 taagttgctt gttgcgcaaa gtccgtatac atgagccacg tttccgtaaa ccactccgtt
    41221 agaaattcca agcctttgat ttttgcgaaa acaccgggaa cgaaggccgt tcccatgata
    41281 taaagaaaag aaagaagcaa actgacactg gtacttctag tgatgaatgt caaagccact
    41341 ggaacagata atgtcgctaa tatcagcaga agcaatacag gaaatacttt tagaacataa
    41401 tttccatagt ttttgattcc ctcaacttca ccaaatccga aagcaagtgt gtaagcaatc
    41461 atggctagcc ccgtaatcaa aaccacgcca agtgttgtga tcaaaagcgt aacgagaagt
    41521 ttcgacacat aataagtcat tcgattccgt cctaaagata acgtattgcg aatcgtaccg
    41581 ttttgaaatt cttccccaat ataaaagcac gcaaatgctg cgaaagccag aagaatccat
    41641 gcagccattt tttccaccat taaaatccct gttgcaccgt tgatgactgt atccgttgct
    41701 tggtttaatt cattgccgta ttttgtatgc atataccatt cgttccaaga ataaaaaagc
    41761 ggaagtacga ccatcagtcc tagtgagacc cacattttac ggctcttcca tacttttatt
    41821 ttttcagcag ttaatagatt aaacatgtgg attgcctcca gttaattgta agaaataatc
    41881 ttccagtttt tgcccaacta ggtggatacc atccacagga acttttgcct ctaccaatgc
    41941 ttgattgatg gctgctactt ggtcaattcc ttcaaaaagc tggataacac ggctagattg
    42001 gacaaaataa tccctataac caagagagtc gagaaccgtt actgcttgtt ccgtcgccga
    42061 agtgtcaatt tttataaatt gacgagattc ttgtgccaat tcttctttcg ataattcttt
    42121 gatcaagtgt ccttcatgca aaataccgta gtgagtggcg atctgtgaca attcatctag
    42181 caagtggcta gaaagcaaga cggtgatgcc ccgttctttt accaaacgtt ggatgatttc
    42241 tcgcatttct gccatccctg ctggatccat cccattcgtc ggttcatcca aaatcaaaaa
    42301 ttctggattc gtgatcagtg catttgcaat agctaaacgc tggcgcatac ctaaagagaa
    42361 attctttgct ttctttttcc ctgtattttc caatcgcatc attttcagca ggtcttcgat
    42421 ttctctatcc gacacaccgc cgttcgctgc ttggatacgt aggttatctc ttgcagttaa
    42481 ttctggataa agagcagggg tttcaatcga ttgacccata cgtcgtcttg ctgcctgcaa
    42541 atccgtcgtt ccaaataatt cgatcgaacc ttcatcaatc gagatcaaac ccataatagt
    42601 tctcataaag gtagacttac ccgctccatt ctccccaatc aaaccataga tcattccacg
    42661 tttgatttcg atcgatactt tatctaatgc tttggcagca ccgtattttt tggttatacc
    42721 agttgctttc aagaccgttt catttccaaa atccatactt atccttcttt cttattgata
    42781 agtgaagtat agttgacggg tataaagaaa gcagccagaa atgtataaag aaagtgtaaa
    42841 ttttccaatt gaatttcaag aaaaatcaga ctgtctcgca gacgtggttg agttgtctgc
    42901 caatcaaaga gcgcaccaaa cagtgctaat ccaagtggac tggccaccat ggtaatcgtt
    42961 gttatcgttg acatcagacg tccttgtata tgcgttggaa cttctcgctg aagccagacc
    43021 atataaaaag caaaagaaaa cattaatcct taaaaacgga cttatgaaaa ataatacatg
    43081 ccgtttggga tttacaatta gctaccaaat aagaggttat gacaaaagac ttttcacaat
    43141 cccttcttcc gaataaacgg tgttctcctg ccaactcctc ggtcacaagt cacaatattt
    43201 atcaaacatg agaagcaatt tttgtaaatt ccttcttatt tcttgaagct gaccacttct
    43261 gtcacaacct cagaagcgga taaataatat tgtttgatga tactttatct ttttcccgtt
    43321 tgtttataga tcgaatgata ttatagacca agaatcctac ctattaacat agctaaaaac
    43381 acaaaaaagg cactttccaa attgtggaaa gtgccagaaa agttaggatt aaggtaactg
    43441 cgctcagcat tgcttcgccc agtacgattc tcgtaactcg tgaactcgaa cgataatcgc
    43501 aattaacgtt ttgagaactg tgaagcttta cgggctttct taagacctgg ttaaacaaaa
    43561 ctctatttta ttgcatttta caggaatgta tttcatgcga aattaataag tctttgttta
    43621 tagagtttta ctagggtata aaaagttgta tgttatcatt acctcaaaac attacctcag
    43681 cttggcgagt tggttgattg aagaataaat catttatcta gatatctcaa taaaaaatga
    43741 tttctaagaa acttgtattt tcgtgttatt atttttttgc cctatcaaaa tttctaattc
    43801 ttctagcgta atatgattat gacacataat cgattcaatt acttcaatac ctattttttt
    43861 cgagaaagat cccttttgtt cactcacaaa aatactttca gaagacatat ataaggaacg
    43921 aagttttgga attagtatat cgtacaaata gaccaccata aaatcgtcct tgcaaccatt
    43981 ctcaaaaaga attaaatttg ctgattgtgt tacatatatc actgaattac attcatggtc
    44041 aattccaaaa acaatgtcta cttcattttc ttttctaatt cgcgcagtta catttttcaa
    44101 actttcacca ttacttataa atgctttagt tactgtaagc tctgatgttt gttcagatat
    44161 tccttttata atattttgtt catcagccac attccccata aatccgccga ttccttttaa
    44221 gtaataattc tcacctaaga gtcgcttgca ttcactgaat tgataaaaaa accagttaaa
    44281 aaaatcttct ggaaaaataa attctgatcg aactttattt atatttttag ctcctattaa
    44341 ttttataact cgattaatgt tcacttgctg cgatgaacat ataactgaat aaattgagtt
    44401 gtttaattca aaaaaacaac tttaacatat gcatcattaa ttcgttattt tttaggtaga
    44461 tattgatcat tgttatctct ctttttttgc ttcccatact ccaacttaga aattgccgtt
    44521 ataaaattta ttttaaaggt gactttttcc cctgaagtat cggtagtagg aatacttaat
    44581 acctcagctt ctgaggaggt atcccaatta tagttatact ttgcatcatc accataaaga
    44641 acaattccat tttttttatc ttgtgccgtt agctttactg gttcgcttac ttttcttgtt
    44701 aatatcttgg actttacatc atcgatattg cttattttat caagcttaaa taatttacaa
    44761 attaatttcg tactagtt
//
LOCUS       DAKBVG010000004        42001 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000004 DAKBVG010000000
VERSION     DAKBVG010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 42001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.004"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            62..709
                     /locus_tag="OUD09_000184"
     CDS             62..709
                     /locus_tag="OUD09_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HCT9691496.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            724..2250
                     /locus_tag="OUD09_000185"
     CDS             724..2250
                     /locus_tag="OUD09_000185"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCT9691497.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(2299..5097)
                     /gene="ileS"
                     /locus_tag="OUD09_000186"
     CDS             complement(2299..5097)
                     /gene="ileS"
                     /locus_tag="OUD09_000186"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCT9691498.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKSYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(5359..6066)
                     /locus_tag="OUD09_000187"
     CDS             complement(5359..6066)
                     /locus_tag="OUD09_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HCT9691499.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            complement(6087..6869)
                     /locus_tag="OUD09_000188"
     CDS             complement(6087..6869)
                     /locus_tag="OUD09_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HCT9691500.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            complement(6918..7205)
                     /locus_tag="OUD09_000189"
     CDS             complement(6918..7205)
                     /locus_tag="OUD09_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HCT9691501.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            complement(7220..7819)
                     /locus_tag="OUD09_000190"
     CDS             complement(7220..7819)
                     /locus_tag="OUD09_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HCT9691502.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            complement(7833..8510)
                     /locus_tag="OUD09_000191"
     CDS             complement(7833..8510)
                     /locus_tag="OUD09_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HCT9691503.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            complement(8527..9768)
                     /gene="ftsZ"
                     /locus_tag="OUD09_000192"
     CDS             complement(8527..9768)
                     /gene="ftsZ"
                     /locus_tag="OUD09_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCT9691504.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            complement(9791..11116)
                     /gene="ftsA"
                     /locus_tag="OUD09_000193"
     CDS             complement(9791..11116)
                     /gene="ftsA"
                     /locus_tag="OUD09_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCT9691505.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            complement(11272..12492)
                     /locus_tag="OUD09_000194"
     CDS             complement(11272..12492)
                     /locus_tag="OUD09_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HCT9691506.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            complement(12507..13595)
                     /gene="murG"
                     /locus_tag="OUD09_000195"
     CDS             complement(12507..13595)
                     /gene="murG"
                     /locus_tag="OUD09_000195"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCT9691507.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            complement(13621..14982)
                     /locus_tag="OUD09_000196"
     CDS             complement(13621..14982)
                     /locus_tag="OUD09_000196"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCT9691508.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            complement(14996..15958)
                     /locus_tag="OUD09_000197"
     CDS             complement(14996..15958)
                     /locus_tag="OUD09_000197"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCT9691509.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            complement(15984..18176)
                     /locus_tag="OUD09_000198"
     CDS             complement(15984..18176)
                     /locus_tag="OUD09_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HCT9691510.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGIAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            complement(18177..18578)
                     /gene="ftsL"
                     /locus_tag="OUD09_000199"
     CDS             complement(18177..18578)
                     /gene="ftsL"
                     /locus_tag="OUD09_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCT9691511.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            complement(18583..19542)
                     /gene="rsmH"
                     /locus_tag="OUD09_000200"
     CDS             complement(18583..19542)
                     /gene="rsmH"
                     /locus_tag="OUD09_000200"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCT9691512.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            complement(19563..19994)
                     /gene="mraZ"
                     /locus_tag="OUD09_000201"
     CDS             complement(19563..19994)
                     /gene="mraZ"
                     /locus_tag="OUD09_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCT9691513.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            complement(20164..20532)
                     /locus_tag="OUD09_000202"
     CDS             complement(20164..20532)
                     /locus_tag="OUD09_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HCT9691514.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            20703..21659
                     /locus_tag="OUD09_000203"
     CDS             20703..21659
                     /locus_tag="OUD09_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HCT9691515.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            21732..21848
                     /locus_tag="OUD09_000204"
     CDS             21732..21848
                     /locus_tag="OUD09_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HCT9691516.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(21903..23585)
                     /gene="recN"
                     /locus_tag="OUD09_000205"
     CDS             complement(21903..23585)
                     /gene="recN"
                     /locus_tag="OUD09_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCT9691517.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(23603..24052)
                     /locus_tag="OUD09_000206"
     CDS             complement(23603..24052)
                     /locus_tag="OUD09_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HCT9691518.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            complement(24159..24977)
                     /locus_tag="OUD09_000207"
     CDS             complement(24159..24977)
                     /locus_tag="OUD09_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HCT9691519.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            complement(24986..25876)
                     /locus_tag="OUD09_000208"
     CDS             complement(24986..25876)
                     /locus_tag="OUD09_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HCT9691520.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            complement(25878..26102)
                     /locus_tag="OUD09_000209"
     CDS             complement(25878..26102)
                     /locus_tag="OUD09_000209"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCT9691521.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            complement(26107..27444)
                     /locus_tag="OUD09_000210"
     CDS             complement(26107..27444)
                     /locus_tag="OUD09_000210"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCT9691522.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            complement(27445..28299)
                     /locus_tag="OUD09_000211"
     CDS             complement(27445..28299)
                     /locus_tag="OUD09_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HCT9691523.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            complement(28396..28845)
                     /gene="nusB"
                     /locus_tag="OUD09_000212"
     CDS             complement(28396..28845)
                     /gene="nusB"
                     /locus_tag="OUD09_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCT9691524.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            complement(28838..29263)
                     /locus_tag="OUD09_000213"
     CDS             complement(28838..29263)
                     /locus_tag="OUD09_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCT9691525.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            complement(29509..30573)
                     /locus_tag="OUD09_000214"
     CDS             complement(29509..30573)
                     /locus_tag="OUD09_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HCT9691526.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            complement(30745..30990)
                     /locus_tag="OUD09_000215"
     CDS             complement(30745..30990)
                     /locus_tag="OUD09_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCT9691527.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            complement(31129..31503)
                     /locus_tag="OUD09_000216"
     CDS             complement(31129..31503)
                     /locus_tag="OUD09_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HCT9691528.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            complement(31586..32500)
                     /locus_tag="OUD09_000217"
     CDS             complement(31586..32500)
                     /locus_tag="OUD09_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HCT9691529.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            complement(32521..33324)
                     /locus_tag="OUD09_000218"
     CDS             complement(32521..33324)
                     /locus_tag="OUD09_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HCT9691530.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            complement(33357..34346)
                     /gene="manX"
                     /locus_tag="OUD09_000219"
     CDS             complement(33357..34346)
                     /gene="manX"
                     /locus_tag="OUD09_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HCT9691531.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            complement(34449..34940)
                     /locus_tag="OUD09_000220"
     CDS             complement(34449..34940)
                     /locus_tag="OUD09_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCT9691532.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            complement(35131..37938)
                     /locus_tag="OUD09_000221"
     CDS             complement(35131..37938)
                     /locus_tag="OUD09_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCT9691533.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            complement(38372..38665)
                     /gene="rpmA"
                     /locus_tag="OUD09_000222"
     CDS             complement(38372..38665)
                     /gene="rpmA"
                     /locus_tag="OUD09_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCT9691534.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(38678..39022)
                     /locus_tag="OUD09_000223"
     CDS             complement(38678..39022)
                     /locus_tag="OUD09_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HCT9691535.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            complement(39049..39357)
                     /gene="rplU"
                     /locus_tag="OUD09_000224"
     CDS             complement(39049..39357)
                     /gene="rplU"
                     /locus_tag="OUD09_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCT9691536.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     misc_feature    complement(39377..39453)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            39639..40280
                     /locus_tag="OUD09_000225"
     CDS             39639..40280
                     /locus_tag="OUD09_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCT9691537.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            complement(40342..41973)
                     /locus_tag="OUD09_000226"
     CDS             complement(40342..41973)
                     /locus_tag="OUD09_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HCT9691538.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
BASE COUNT        11828 a         9153 c         7147 g        13873 t
ORIGIN      
        1 ttaacgtttt ttttgttaca cttactaatg atggtttttt accatgaaag gatgatcgaa
       61 tatgacacca aatcgagaag attatttgaa aatcatttta gaactaggtg gagatacaac
      121 gaaagtcaac aataaacaaa tcgtatctag tctagatgtc tcagctgcat ctgtcagtga
      181 aatgatttct aaacttgtga aagaagagct tgtagaacat tccccttatc aaggtgtaca
      241 attgactgca agcggactgc aaaaagctag cagtctgatt cgcaaacatc gcttatggga
      301 agttttttta gttgaacatt tagactatag ctggaatgaa gtccatgatg atgctgaagt
      361 attggaacat gtgacttcgg aacatcttgc agatcattta gaaaactatt tgaatcatcc
      421 tgaacattgt cctcatggag gatcgattcc taaaaaagag gaagttgtcc atgaagaacg
      481 ccgacaaaca ttggagtcct atcctgtcgg tacaaaagtg cgtatcgcac gagtgcttga
      541 tgaaaaagaa ttgttggatt acttagtcac tattgacctg aatatcaacg aagaatatga
      601 gatcgttgac atcgcagctt atgaaggacc gatcactatc caaaatgata gaaagaaaat
      661 ccccgtcagc tttaaggcgg ccagcacgat ttttgtcgat aaaatataaa ggagtgtatc
      721 cacatgtcta atgaaaaaaa tgtattgttt acgatattcg gcggtacagg tgacctagcc
      781 cagcgtaaat tgtacccgtc tttgttccgc ctttacagaa aaggaaattt aggcgagcat
      841 tttgctgtaa tcggaaccgc tcgccgtccc tggagcgatg agcactaccg tgaagttgtc
      901 aaagaaacaa tccgtgcatt gaatccttca gatgaagaag caactacctt tgcaagccat
      961 ttttactatc aatcccatga tgtaaatgat tcaacgcatt atcagacatt aaaagaattg
     1021 tctgaccgat tagatggaca ctatcaatta aacggtaacc gtgtctatta tttggctatg
     1081 gcacctcaat tttttggaac gatcgtttct catttgaaat cacaaaatat tatgacaaaa
     1141 aatggcttca atcgtttgat catagaaaaa ccgttcggtt ctgattacca gtctgctttt
     1201 gaattgaacc agcaaatcag agaagtattc ccagaacaag atattttcag gattgatcat
     1261 tatttaggaa aagaaatgat ccaaaacatc tcagctatcc gttttgccaa taatattttt
     1321 gaggctcaat ggaacaatcg ctatatcgac aatatccaaa ttacttttgg cgaaaacctc
     1381 ggtgtagaag atcgcggggg ctactacgat catagtggcg cacttaaaga catggtgcaa
     1441 aatcatattt tacaggttgt agcattactt gcaatggaac cgccagtagc attttccgaa
     1501 aaagaaatcc gtacagaaaa gatcaaagca ttgaaagcta tccgcattta taacgaagaa
     1561 gaagtgttag aaaacttcgt tcgcgggcaa tatgctcaag gtgaattgga tggtgtacaa
     1621 ttcaagggct atcgcgaaga agacaaagtg gatgctcaat cagagactga aacattcgtc
     1681 gcaggcaaat tcactatcga taacttccgt tggtcaggtg ttcctttcta tgtacgtaca
     1741 ggcaaacgtt tgactgaaaa agggactcgg atcaacatcg tattcaaaca agttcctgtt
     1801 aatgtattca aaacatctgt cgatgaacca tgtgacgata cgacacttcc acctaatgtt
     1861 ctaacgatct atattcagcc aactgaagga ttttctttat ccttgaatgg gaaagaagta
     1921 gggcaaggat ttgaaacgga accaatcaaa ttggaattta gaaatagtgc agaaatggtt
     1981 gaaaatagcc cagaagctta cgaaaaatta ctattagatg cgttgaatgg tgatggaacg
     2041 aacttctctc attgggaaga agtcgctcaa tcttggcata tcgtggatgt cattcgaaaa
     2101 gcttgggacc aaacacaacc ggattttccc aactacaaag ctggatcaat gggacctaaa
     2161 gcagctttcg aactacttga aaaagacgga ttcgaatgga tctggcaacc agataactgg
     2221 tatcgtgaac gtggaaaatt aaacgattga taacaaaagg gggtgtgaca aagaacatgt
     2281 cacaccccct tttccctttt acttttcttc gaatccttca gctacagctt caggatagaa
     2341 gtcttcaacg atttttgcac aacgaccgca tagatgagga agtttttcat ctactcctac
     2401 atctttacga acttggcgac agcgatcgca agtttccccg tctgcttttt ccactgtaat
     2461 agcaacatca tcaaatacca tagcgttatc tggcacttgt tcatctgctg ccataacttc
     2521 aaagtagtct ggtgaaacga tcaataattg tgcaatatct gcatccaaag cttttaacat
     2581 ttcacgaact tgttgttttg gataaacagt tagttttgct tccaaagatt taccaatcaa
     2641 tttgctgttt cttgcttctt ccaatgcttt taagacatta tcacggaagt ccatgaaagc
     2701 agtccaggta tccatcaatt cttcttggtt tgcaaactct tcataaccag gaaactcgct
     2761 taactgaacg tactcctctt cttctttcag gaatgaccag atttcttctg ctgtatgcgg
     2821 aatgatagga gtcaataatt ttgtcaaagc tacagctgtt tgatagaaaa cagtttgcat
     2881 gcaacgacgt tgataatcat cttctgcttc aatataaact acatctttag caaaatccag
     2941 gtagaatgac gatagatcaa ccgtcaagaa gttcataact gtacggtaaa tatgcatgaa
     3001 gttatatttg tcgtaccctt cttctcggat ttctttgatt acttggttca atcgaacggt
     3061 catatatttg tctacagaac gtaaatcatt gtacgaaaca gtatcttttt taggatcgaa
     3121 atcttctgta ttcgctaata agaaacgcat cgtatttctg attttacgat atacttctgc
     3181 cacttgactt aagatatcca tagaaacacg aacatctgct tcataatcta cactagatac
     3241 ccataagcga aggatatctg caccaaattg tttgatcact ttttctggtg caatgatgtt
     3301 tcctaaagat ttactcatct tacggccttc accatctaaa gcaaatcctt gagaaataac
     3361 tgatttgtat ggtgctacac cattgatagc cacactagtc gtgatacttg agttgaacca
     3421 gccacgatat tggtctgatc cttctaaata catatctgct gggaatgata attctggtcg
     3481 ctgacgtaaa acagcttcat gagaagaacc agaatcaaac cagacatcca tgatatcttt
     3541 ttcttttgta aattcgccat taggagaacc tgggtgggta aatccttcag gaagaagttc
     3601 tttcgcttct ttttcaaacc agataattga tccatgttca gcaaataatt ttgccacatg
     3661 ttcgattgtt tctggtgtga tgattgcttc tccattttca gcatagaaaa tcggaagtgg
     3721 tactccccat gcacgctgac gggaaattac ccaatcgccg cggtcacgga tcatattata
     3781 caaacgtgtt ttaccccatg ggataaccca ctctactttt tctacttcat ccaaaatatt
     3841 ctgacggaat ttatcgatag atgcaaacca ttgtggagtt gcccgataga tcacaggctt
     3901 tttcgtccgc caatcgtgag gataactatg agtaaagaaa tctagcttca gcaaagctcc
     3961 tttttctttt aatagatcag tgatcatagg atttgctttg tcataaaaga taccttcaaa
     4021 tccaggtgct tcttctgtaa acaatccttt tgagtctaca ggtgacacta cagatagttt
     4081 atatttgcta ccagcgatat agtcatcttc cccatgtcct ggagcagtat ggactaatcc
     4141 agtaccagcg tctaaagtaa catgatcgcc taaaatcaca agtgattcac gttcatagaa
     4201 tggatgactt gcagtcatat attccatttc ttgtccagca aattctttta gaactgttac
     4261 attttcccaa ccgatttctg attggactgt ttctagcaaa tctttcgcta cgacaaactt
     4321 acggccatcg gcttccacca atacatacgt aaattttgga tttacagcaa taccttggtt
     4381 tgctggcaat gtccatggtg ttgtcgtcca aatgacaaaa gctgtatctt catcaagaat
     4441 atctttgccg tctttgacat tgaaagctac aaaaatagat ggagatttta catctttata
     4501 ttcaatttca gcttccgcta aagaagactc acttgacgga gaccagtaaa ttggtttcaa
     4561 tcctttgtaa atatagcttt tttcagccat tttaccaaat acgcgtactt gtgcagcttc
     4621 ataagaagga tctaatgtaa cataaggatg ttcccaatct cctgcaactc ctaggcgttt
     4681 gaaatctgct cgttgcttat ctacttggga aagtgcatat tcacgacatt tttccaagta
     4741 ttctgccatg gacatttctt tacgtttcac gcctttatta gccaatactt gttcaatcgg
     4801 caatccatgt gtatcccatc ctggaacata tggtgcacga tagccagaca ttgattttga
     4861 acgaatgata atgtctttac tgattttatt caatgaatgt cctaaatgga tattaccatt
     4921 ggcatatgga ggtccatcat gaagaacaaa gctaggtttt ccttcgttca actcttgtct
     4981 tttttggtag attcgatttt cttcccattc tttttgccac tcgccttctc ggtttggcaa
     5041 attaccacgc atagggaatt ttgtttttcc taattgcaag gtttctttca ttttcattca
     5101 accatacact cctttatttg ttttccggac acaaaaaagc atgttcagcc gcaagggacg
     5161 aacatgccgt ggtaccaccc aaatttagtt gcatctatct agtgcaaact ctctgtgacc
     5221 aataacgagg tcagccgctg cttcttactc taatttcgga aaagcaggtc tcaagtagag
     5281 gatctttcac catgcagtgt cttaccgaac ttccactctc atcggctcgc tttaagaata
     5341 acaaagtgct tttctgttct actcgttttc attttcacca ggcacgatcg agatatcaat
     5401 agcctgtgta tcaaatgccg tcataggtgc ttctatgact cctgtgtctg agtttacatc
     5461 agtatcattt tcattgtcaa gctgttttgc taaaacttct ttgatcactt catggctgtc
     5521 agtcacgtag ctggagaatg gttgtaaaat ttcatcccat tccggacttt tcacttgttc
     5581 taattgagac tcaagcatca agctgatacg ttgatggaat actcgtgttt ttttcttaag
     5641 atcttcagtt tctgtcgcaa gctgtcttgc tttttctgtt gcatctgtca agatttttct
     5701 tgctttttct tctgcatcag ttgttaattg atgtgcacgt ttttcagcgt tcgcaactaa
     5761 ctcatctgct ttgttttgtg cagaagtaac gatcacttcg gattccttgc ttgcacttgt
     5821 ttttacttta tcagccgtat cttgagctac gatgattgat tggttcaaag catcttttaa
     5881 ttcattgaag tactcaagtt tttcttcaga atgtttgact gctttttcta attctcgatt
     5941 tctttgagtc aattcctcat agtcacgaac aactaaatct aagaaatcat ctacttcgtc
     6001 ttgattatat ccccgcattt ttgtcgggaa tgtcttattt tggatatcta atggagttaa
     6061 agccatgagt aaaccacctt tttattttat ttacgtaata caccgaacag taccctgtac
     6121 ttttcttttt tcgtcttccc ctcaagttct tggatctgga ctctgccata acctctgact
     6181 gaaataatat ctaatagttc aagtacaaaa tcaggtcttg tattttctgt ccagttcact
     6241 tttactttac cagcttcgat cagctgcttg gaccgctgtc tggaaatatt ataaatctca
     6301 gagatcacac tgtctagtcg catggaacta actgttcctc tttcttgtgt ccagctatct
     6361 ttcggcataa tcatttctgt gtatttgatt ggttccaagc gtacagcaac ctttccaatc
     6421 tttgttactt gtgcagcaac aaaatgatcc acctcatttg caataaatac ttgccagcga
     6481 tcaccgtccg agatgatatc gccaaagtaa gagcgtttga tccccgttcc cactaaagtt
     6541 ccaaggattt tcccatgact taggctggca aatttgctag gataatggat ttcatacaat
     6601 gctgtttcaa aatcttcctc tacaggttcg taataatcgg ggaaaatcaa acatcttttt
     6661 ctttctgcct gttcatatcc gccataaaac atgaaacgca aatcactgct ttgtcgaatc
     6721 agtgtttcta aaatatagga ttgtcttggg tcaagaaatt cggtcagata gggtgcatat
     6781 tgtccttgaa cctgttcaat ccaatcgcct accgaatcga taaatgtacg ttcatctgcc
     6841 cgaaagtgct ggtatacatt tgcatccaaa attttcccct cctatctatt ctgagaataa
     6901 agattttgtt gcttcaatca ataaactaaa tggacagtta tccatataac gatctgtgct
     6961 aaaccttgta cggctaagtt caagatcagg attgccccta aaattgtgaa atccaagggt
     7021 ccaatcctca aaggcagacg atcgaacaaa ctgatatacg gttcgcagat cttagctaat
     7081 aatcgtccta gccctgattg atacccgcct ggaaaccaag agagcaatgc atataccatc
     7141 aagataatgc tatagaaata ggctgcccgt tgaagtaaat gaagtaatag tgcaatagta
     7201 taaatcgtag ttcctcctct tacagatcaa acaaattcgc atctgcaaaa gattgagcgg
     7261 ttgaactgtc aatctccatc ccagtcggtg tacaaagaaa aatctcatct ccaacacgtt
     7321 tgatatctcc atctagtgca taaactgttc ctgttagaaa atcaacgatt cttctcgctt
     7381 gtttttcttc tactaaatga aaattcacta aggttgcttc tccagcaatg attcgtttag
     7441 caatcgccat agcttcagaa taagctcttg gttccattac agtgattttt ccagcaagat
     7501 ttgctttagg ttgtgtagag gttcttgaag ctgttctttc tttcgctaca gtttttgtag
     7561 gtgattgatg tagttcaatt acttttttct tttcagccgg ctgaatactt tcatgcgtta
     7621 caggacgtga agtcgccgtt ttttgttttt catgagtatt cgcagcagat acagaatgag
     7681 ttgattgatt ctgtgttgta ttttttttag ttggctgtac agcctttttt gttgtttcag
     7741 gataatcttg gtattcgtac tcttcctcat cagctaatcc gaaaaaatta gcaattgctc
     7801 ctctgtttaa aagcggcatt tttgttcctc ctttattcgt ctttgaatag cgctgttcct
     7861 acacggataa atgttgcacc ttcttcaatc gcaatttgaa agtcttgact catccccata
     7921 cttaattcac tacatggtgc atgggcataa ccttttgctt gaacaagatc acgcaattct
     7981 ttcagcgtac caaacacttt atggatttgc tcatcagaag cttctttagg cgccatagtc
     8041 atcaacccaa caaccttgat ttttgtgcag gcagctaaag attccacaaa cggtaaaagc
     8101 tcatctggtg aaattccatg ttttgattcc tcaccagaaa catttacttc aataaaacag
     8161 gataattctt tctctaaccg cttctggatt tcttctgcta atcttaaact gtccaacgca
     8221 tgaaagtaat caatttcatt tacaatcaat tttacctttc tgcgctgtaa attaccaatt
     8281 aaatgccact ttatttcagg aaaatcaaat aaatcttgtt tcttttctaa taatttatct
     8341 actcggtttt ctgcacaatt cataacgcca gctctaacca attcttcagc ttgagcggat
     8401 tcaacagatt ttgttacagc tactaatgta acttctttcg gatcacgatg aacaagagca
     8461 cacgaatcct gaatttcttg cttgattttc tgcaagttgt cagcaatcat gtgctcttcc
     8521 tctcctttat tttttacgac ggaaaaacgg tggcgtattt aattcatcat cgccatgaga
     8581 ttttgtttct tcgcggttga atgtatcaaa ttcttttttt tcgatattgt caaaattgga
     8641 atcatcaact ctaggacgaa cattttgttc acgtctgata tcccaatcgc caaaactgtt
     8701 ttcttcttct gaagaagttg gtttagcttg atccatatct aatacagtct tttgagcagg
     8761 tgattgcatt tgtgcttgtc tagctggtcg gctagacttg cgttcctttt ttgattcatc
     8821 gatacctgtt gcgatgaccg tcacacggat ttcatcgccc atttcttcat tgatagatgt
     8881 acctagaatg atatttacat cgccagttgc tgcatttgct acgatatctg aagcatcttg
     8941 tgcttcgaac aatgtcatat ccaaaccgcc tgtgatgtta agaagtactt gttcagctcc
     9001 atcaatagat gtttcaagca atggagagga gattgctttt ttcgttgctt ctactacgcg
     9061 atcttctcca ctagccacac cgatccccat caatgctgta ccttggtttt ccatcactgt
     9121 tttcacgtca gcaaagtcaa ggttaacgta acctggcgct gtgatcaaat cagagatacc
     9181 ttgtacacct tgtcgtaaaa cattatctgc ttcacggaaa gcttcaagca tcggtgtctt
     9241 tttatctaca acttctaata aacggttatt tgagataatt aataatgtat ccacattttc
     9301 tttcaaacga gcgatacctt ctgcagcaaa acggccacgt ttcggtcctt caaaagtgaa
     9361 tggacgtgtc acgacaccaa cagtcaatgc gcccaattct ttcgcgatac ctgcaacgat
     9421 tggtgcagca cctgtaccgg ttcctccacc cattcctgca gtgatgaaaa tcatatctgc
     9481 accgtctaaa gcttcgcgta aggattgttc actttcttct gccgcttttt gtccaacttc
     9541 tggctgagaa ccagcaccta aaccacgagt atatttgggg cctaattgga taactgtttc
     9601 agcttttgaa ttttttaatg cttgcacgtc tgtgttggct gtaatgaatt caacaccttt
     9661 aacgttttct tcaatcattc ggtttacagc atttccaccg ccaccaccga ctccgatgac
     9721 tttgattact gcgccgtcat tgatattatt atcaattgag aattccatat cttattcctc
     9781 ccaggatgaa ttaatcaaaa atatttgtaa agaaaccctt gattttattt tttacatttt
     9841 ctccggactc ttttggttcc ggctcatcgt agattgtttc ttcaggtgct tcatagctct
     9901 catatgtgtt tgttgtttct tgttcaacta ccatttgacg agctgctgag gtttcacccg
     9961 taacagcaat ttttgccaat tgatacactt cactcaagtt tgctgaataa tccacgatac
    10021 ttattacatt tgtaaatacc gggtttctca gccccatgtg atttggaaca tacaatttta
    10081 catttacgcc gaagatttct tgtgccaagt ccacaacacc aggaagacta gcagctccgc
    10141 ctgtcaatac gacgccacct ggcaactcta atgcttcgat ttggtccaat gcatcttttg
    10201 ctttgttgaa aatctgttcc attcgagctg aaataatttc tgacaagtag cgttcatcga
    10261 ctttcactgg ttctgactga ccaatcacat ctactggaaa ttcttcatct ggtgatgtcc
    10321 gttctgggta tgcatccccg tagtttattt tcaacgcttc tgcattgtta aatgacgtat
    10381 ttaatacaat agagatatct ttagtgacaa attcgccacc ttcttggtca agattggtaa
    10441 acttcaattg cttatcatgc ataacagccg ttgttgtttg gccgccaccc atatcaatga
    10501 cgatcgtacc aaaatctttt tctccgtcag aaagaattga ttcagtcagc gctaaaggag
    10561 taatcaccat ttcgttgacg attaatccgg cattttccac gcatttacgt atgttatgga
    10621 tgatcgtttt aggtcctgta aacaacagac cgtacatttc taatcttacg ccgatcattc
    10681 cacgaggatc tttgattccc tcgaacccat cgacagtaaa atcttgtggc aatattgaca
    10741 cgatttgacg ttcaggaggt attgaacgaa caagggcagc tgaagccaca tttctgacat
    10801 cttcatcggt gatttccttt gaatctccgt taactgcaat cattccctgg caattttcta
    10861 cttcaagcat gtttgctggc aaaccgacac ttacgccttt gatctggata cctgcttttt
    10921 cttctgcttg tcgtactgct cgttgtatcg cttggaccgt tttatcgata tcaacgataa
    10981 tgcctcggtt aatcccttct gattttgcgt ttcctactcc aataatattc atttggctgt
    11041 cgatatattc agccacgaca actttaacag atgtcgttcc aatatcaagg cctacataca
    11101 ttcctgtttt tgccatgaat gggattccct cctacttact attccatata caagcgaaca
    11161 actagcgtcc gttttaaaat atacttatta atagatatat tcactgcttc caattctacc
    11221 ataaattact ggtttttaga aagtacattc ttacttttta aagttgtttt atcaattttc
    11281 atttaaactt gacggagttg tacttggatt atcttctatt tgacttgttt cacttgaatc
    11341 atttgaatca gatgatccgt ttgtgctaga cgatccaggt gtttgtgctt tttgcgtttc
    11401 tgcctcttca cttgattcgt ttgtttgatc cgcattttct tcactttcag ttgtttcttc
    11461 cgattggccc gattcttccg tttcttgagt gctaccgtaa ggatgcgaga agataccaac
    11521 ttccatgtcg atcacacctt tttcatccat atctttggct acttgcgaat aatatttcat
    11581 ttggttcgca agattattga tatttacaat aacttgattg ccgtcattca tattcaattg
    11641 gatcagattt ttattgcttg ctcttggcgt gtacttgatt tcagaaattg ctttttgtaa
    11701 ttctgatgat aactgattgt attgctttgt taattctttg atttttgcag agtctttgaa
    11761 ctcttccaag atagggagat tttttgtagg atttgctact ttttcatcta atactgttcc
    11821 attttcaatc actgggtagt attgattgtt ttttgcgatc aaagcaatct ctttatattc
    11881 tgttacatcc aaatcgaatt cattcatccc tttgaaatgt atatttgctg tttcgattct
    11941 tggctgtgct tttttaaggc gatcgatata agtagatcga tgaaaatact gtccccaaac
    12001 attttctcct aatgacaatt ttgtatcaga gatgatttct tggctgttga ctgtcttatt
    12061 gcctgatact gcaactgctt gtaatttgct ataaggagaa acataataga tcaaaaaaat
    12121 cagcggtata cccaatacag tgatgatcaa agctaaccga cgaaatagta ctttgtgccg
    12181 ttgacttttt aaattgggca gccggttcaa aaaagaaccg ttatatggtc caccgttttt
    12241 cttttgcttg atctcttcaa aaaattcgat ttttttctcg gactggcttt cttcttgctg
    12301 ttcctcttga accgtttttt cttcttcaga atctgaaagg ggtgtatcta atgttatgtc
    12361 ttcttttttt ggctcttggt ttttcatctc ttgttctgcc agatattttc ggttagcctt
    12421 ttgccaagga gtcaaattgt tttcatcaga agacggatga gacaattttt ttggttcttt
    12481 tttcttactc actaagacca ccgcctttat tttttgatta tttgttttac taagtcataa
    12541 agacgttttg aagcatcagg gattccttgt tctttagaag ctcggcacat ttgcttttgc
    12601 aattcttcat cacccatgat ttcgttgact gtctgactta gtgattcacc tgttagttca
    12661 ttatctgcaa tcatttttgc tgctcctgcg tgaacaagac tcattgcatt tttagtctga
    12721 tgatcattcg taacatatgg gctgggaacc aacactgccg gtaaccccag agctgtgaat
    12781 tctgcgatcg aagttgcacc agcacgcccc accagcagat cactggaagc catgacctct
    12841 gccatatttt tgatataagg ctggacacta atattttgta tttcaggatt tattttattt
    12901 aggatctctt cataatatct atctcctgaa gcatacaaaa cttggtaaga agaaggaata
    12961 tattcactta aaaaaactga tactgcttga ttgattttaa gtgctccttg actaccgcca
    13021 aaaatcaaga ctgttttctt ttctggatcc aaatcaaatg ttttcaaaat atctgatttt
    13081 ttagtatctg ctacttcttg tgctcgagga ttacctacaa gaactgcttt gttttcagga
    13141 aaaaaggaag ccgcatcttt aaatgaaaga gcgatccgat ccacatatcg gcttaaaaat
    13201 ttattggtaa cacctggtac actgttttgt tcatggataa ctgttggaat acccattttg
    13261 gctgcagcgt acactaccgc tccggaaaca tatcctccag taccgataac gatatctggt
    13321 tgaaaatcac tgaggatttt ttttgcttga cggatactct taagaaacaa tcggaccgtc
    13381 ttgatgttat gcatggaaat ttttcgttta aatccttgaa tctccaaggt atggaaaggc
    13441 attcctgtat cagggacgat tttgttttct agacctcgtt ttgctcccac atacatgaat
    13501 tcagcattcg gttcttgagt ctttacataa ttgacaaagg caagagctgg ataaatatgt
    13561 ccccctgttc ctccgcctgt aaccaaaatt ttcatttttt atcgctcgct tctacttttt
    13621 ctattttaat cggttcacag cttccatgaa tctgtttcct ctagtttcaa aatttgggta
    13681 ttgatcccag ctggcattcg ctggtgataa taaaactaca tcaccatctg tactgttttc
    13741 taatgctaaa ggaactgctt gttctacaga atcggctgtt actattgtct cgatgcctgc
    13801 tttttttcca gcatccatca acttttcttt tgtttggcca aatacgacca ttgctttgat
    13861 tccttttaat gaaggaatta attcatcaaa tgtatttcct ctgtctaatc cgccagccaa
    13921 taacacgacc ttagacgttt caaatccact tagagccatt ttagtcgcta aaatattggt
    13981 tgcttttgaa tcattataaa attttctgcc ttggatttct cctacatatt gcgtacgatg
    14041 tggtacgcca tggaaaaaat gcaacgtttc tcgtatagct tcatttgata taccatacaa
    14101 ttttgctaca gaaatagcag ctagtgcatt ttctacatta tgcttacctg gtacgccaag
    14161 ttcagatatt tccattattt tttctttttt atagtatatt gagtaatcgt cagcatacac
    14221 accgtcttcc aacacttctt ttgtcgagaa aggcagtacg cgtgcttttg ttctttgcgc
    14281 taattcttgt agttctgact gattccagtt aaggatcaga taatcttttt ctgtcatgtt
    14341 tttttgaagg ttccatttag ctttgacata ttcttttctt gtaccatgat aatcaatatg
    14401 tgcttcatag atattggtaa tcaccgctat atgagggcgg aaatctgtga ttcccatgag
    14461 ttggaagcta gacaattcca tcacaatttt atcttctgat tttgcttctt gcgctacacc
    14521 acttgctgga tacccaatat tacccgctaa acgtgcaatt ccttgatctg ctcctgcatt
    14581 cagtagtagt cctgtcatcg ttgttgtcgt tgtttttccg ttcgttcctg taatcccaat
    14641 aattgggcat tccgctactt cgtaagctag ctcaacttcc gtaattaccg gaatacccat
    14701 ttcttgggct tttgctacta aaggatgact gtagggaatc cctggatttt tgaccatcaa
    14761 tgaaaattct tcatccaaca attcgattgg atgactacct gttattactt ttatccctaa
    14821 agaaagcagc tcttgtgctt ccggattttc gtcaaaaggt tttccatcat taacggtgac
    14881 aagtgcgccc aattcatgta ataatttggc cgcacttact ccgctttttg cgagtcctaa
    14941 gacaagcact ttcttatttt catatgttga tatttttttc atttcccgtc atctcctacc
    15001 aaacgatcca taatgtgatc aacgaagtca caatgctgat caaccaaaaa actgtatcaa
    15061 tcttccactc cgaccatccg cacatttcaa agtgatgatg aattggagac attttaaaaa
    15121 ttcgttttcc tgtaagctta aaggaagtaa cttgaagcat cacacttgct gtttccataa
    15181 catagatcaa accaatcaaa agcaatgtcc attcttggtt tagcattata gaaattgccg
    15241 ctaaaagtcc gcccaacgct aatgaaccga catctcccat aaaaatcttg gcaggttttc
    15301 tgttgtagac gaagaaaccc agtaaacctc ctaaaacact taagcaaatg attaatacat
    15361 catactgatg ctgatgccaa gcaattagac cgtaagctgc aaaagagata cttcctaagc
    15421 cagcaactaa tccatcgatg ccatctgtca gatttactgc atttgaaaat ccaacaagcc
    15481 agaagagagc gaaaatacca taaatgattc ctaaaggtag atctagtccg aagaaattca
    15541 atacacctgg ataaccttca gaacggtaaa caagataaaa gatgattccc cctagaatct
    15601 gtccaatcaa tttctgcttc gagttcagtc ccatgtttct tttcttgaat atttttataa
    15661 aatcatcaag aaagccgatt actccgtata acgctaatac aaaaagcaag atgaaaagag
    15721 taggagttag ctgtttttgc caagctccaa cccaaattcc tgtcaaaata gagccgatca
    15781 gaaacactaa gccgcccatt gtaggagttc cggctttgct attgtgccac tttggtccct
    15841 cctcacgaat cgcctgacct tgctttttca tttgaaaata accaataaat agcggcattg
    15901 cggcaattgt cattgcacaa ctgctgacaa tgggaatcaa tgcttgtgtc cattccatgc
    15961 tcttttctct cctatttctc ttcttattct tctaaagtaa aactaagttt gtcttcagtg
    16021 attttttcat atggagcaag ttcttgtttg acacaataac catctccatc aaaactaact
    16081 tcaataccga ggattttccc cagcttcatg atatctgctt ttgaccatcc tgttacgtca
    16141 ggcatcagct tatcaccacc agtatatagg atcagttttt ctccagagat caactgatca
    16201 ccgttggcag tagattgttt ttgtactttt ttaccattac cgatcactac aggttgcagt
    16261 ccactttttt gagcatcagc tgctgcaacg tccgcttcaa gattacgata atcggctacg
    16321 gatacttttt ctgtttttgt ttctgtatct gaatcttctt cagtttcttt aaaatccatt
    16381 gcccgtttca tcaatggatt tgcgatttta gccaatgcca ttcgatcata tgtcttaggg
    16441 tgtttcattg ttagatacaa gacatagtct ggatcttcag aaggaaccat ctccacaatc
    16501 gaatacaggt aggctgtatc tcctgtttga tatccccctg tatcagaagc gatttgggct
    16561 gttccggttt ttgccgatac attgtatcca ggtacactat atacaccata agcaataccg
    16621 tagttttcgc tttcgacaac atctctcata tattcacgga ctttttctgt tgtttctttt
    16681 gaaaaaggtt gccctagaac ttctgtttga gttgttcgct cttctccagt ttggggatcc
    16741 accactttgc tgatatatcg aggttgaatc attgtaccgt tattcgcaat agaagtgaaa
    16801 gctttcatca tttggaaatt cgtcacacct accgcttgtc cataagcact cattgctcgg
    16861 tcgacaatat ttaatgtagg taaagcaccg tttacttcat catctaatcc agaatgagta
    16921 ctttgtccaa atcccagttt ttgtaaatag ttataccatc gtccgcctaa tcgctgttcc
    16981 agtatgacca tcccgacgtt acttgaccat gaaagagctt gacgcatggt cagcacacct
    17041 ttttcaccaa agtcatggtc attgatcgta gcatcttcta cttggatttt tccagattgg
    17101 aaggtttcat tctcgttgaa ttctccttct tcgatagcag ctgcagtagt gaaaactttc
    17161 atggttgatc ctggttcata actatcttgt accaagaaat tacgccagat agcatctttt
    17221 ccagtcaatc cttccattgt ttcaggattg aaagtaggtc gttgtcccat tgccaagatt
    17281 tctccggttt ttgctttcat caggactgct gtcaattctt ctggctgata ttcttcattt
    17341 acttgatcca tcagtgtttc taaataactt tgtagtctgc tgtctaaagt cgtgtagata
    17401 tcttgtccgt cgacagcttt ttcttcttct gccactgtac ctggcagagg attttgaaaa
    17461 ttatcttttt gataaataat cttaccatct ttaccgctca agatatcatt gtaagcagac
    17521 tccaaaccta gtttaccaac taagccattc tcatctttgt ctggaactgc gtatccaata
    17581 aaatgagaag aaaagacgcc atttggatac atccgagcct gatggtcgac aaaataaaga
    17641 ccagcgacac cttctttttt catgtccgct tcgatgtttt gcttggtttc ttgggagata
    17701 tttttcccat agctaccaaa ttcaacttga tacagctctt tttttgcgcc gtcctctaaa
    17761 atattcagtg catctttttt gtctatagaa agattcttat gcaggatttc tgctatttta
    17821 tcaaaattct tttcttcaac atacagtttt ttatccccag aggtatatgt ttttgagagt
    17881 atcgctttaa ttgaataaga ggaggcatct tctgcgagcg ccacaccgtt acgatcataa
    17941 atcgttcctc tttttgcttt tactacttca cttccttggt acaactgttt tgttttttca
    18001 gctaatgagg taccagcaac atgacctcca ataacgatat acgagaaacg gacggcaaat
    18061 aaaaagaaca atccaatact cgtagcaaat aaaatgatac ctactttttt gcgattgttc
    18121 atcggattta gatttttctt ttccataaac cgccgaattt tatttttaag actcatttac
    18181 ttcactttcc ttaaattgtc gtcattaata gacaatcctt tttcttctgc gatttgttta
    18241 atccgttcag tacgagaaag ttcgtttttt tcttgttcta aactcgtttt ttcttgcttt
    18301 ttatcattga tttctgtttg tattgacgtg atttcttttt cagctccgct gatatttgtg
    18361 cgcagcataa ctgttaaaac acataaagca acaatagcaa tcgccacgct tgccacagca
    18421 attttttcca aagcagaaat ctgctttaat cgttttgccg gtgattgtgg aagaatgatt
    18481 tcttgattcg aagaagaatc aggcatttgt tgttccggct gatccaccaa ttcaggtaaa
    18541 tcatattcgt aatcttgcat tttctttaat tctgccatca ttctactcct ctttcattct
    18601 tattttttct gcaatacgta actttgcact gcgtgatcga ttgttttctg ttagctcttc
    18661 ctctgaagga aggattggtt tacgagtaat gactttcagt tctggttgga attcgtcagg
    18721 tatgacagga agtcccggtg gtagatcttg taccgtacta tattctttaa acatactttt
    18781 gacaattctg tcttccaatg aatggaatgt gattacgctg attcttccat tgactttcaa
    18841 taaccggatt gcctgttcta aagaggcctc ttcggcaccg agttcgtcat ttactgcaat
    18901 ccgcacagct tgaaaaattc tttttgcagg atgcccgcct tttcttcttg cgggagctgg
    18961 gatgacttgt ttaatgattt cgacaagctc accagttgtt tggattggtt ttgtttttcg
    19021 tactcgttcg atttcacgag caatttgttt ggaaaatttc tcctcgccat agcggaagaa
    19081 aatctttact aattcatggt aggaatattc attaattacg tgataagcgg ataaaggagc
    19141 tgactgatcc atgcgcatat ccaacggtgc atcttgatga taactaaatc cacgttctgc
    19201 ttcatctagc tggggagagg aaacccctag atcatacaaa atgccgtcta ccttatctac
    19261 atgctcactc aagcgttctt ctaattctct gaagttcgct ttgataaatg tgaccatacc
    19321 ttgttcgaca taagaagcta aacgcagctt ggcgtgatcg attgcttttt gatcttggtc
    19381 aaacgcatac agatgccctt tagaacttag ttggcttaat aaatattcac tatgtccggc
    19441 accgccgagt gtacagtcta cataaacgcc atccggtttg atgttcaatc catctaccgt
    19501 ttctttcaac atgactgtat agtgttgaaa tgtttcagtc attagtcagc ctcttcctat
    19561 tcttatagtc cgaaatcaat catactttca gcaatttcgt caaagttctc ttctgctgct
    19621 gcagaaaaat cttgccatcg tgtctcatcc caaatttcaa ttcggttaga tacaccgatg
    19681 actacgcaac cttttgtcaa agctgcataa tttcttaacg tgctgggtat attgattcgt
    19741 ccttgtttat caagttcgca ttcagtggca gccgaataga agaatcgcac aaaagtacgt
    19801 gcgtcttttt tagccaacgg catttcgttc agtttagctt ccagattttc ccattcgctc
    19861 atcggatagc cgaaaagaca gccatccaat ccgcgtgtca gaacaaactt ttcgccaagt
    19921 tcttcacgaa gttttgaggg cacgattaaa cgtccttttg catcaatatt atgttggaat
    19981 tcacccatca acattgaaag ccccccattc ctccactaac taaaattagt ctaccacaat
    20041 cccccacttt ctaccacctt ttctgggaaa tccgggaaca acttttttta ttcataaaca
    20101 tttttttaaa aatcaagctg caaaaagcct tttaaaagac tttttacagc ttttctattt
    20161 ttttcatagg taagaaagta tgttcacaat gatcaagaca gcgtacaaga cgattgtcaa
    20221 cagaaaaacc aaacgccaag tcatcttgaa ataacgcgga taaataattt cttcataata
    20281 atatgcttgg aaaacagcaa tacaaattcc cattagcaaa acagaaataa agaaaaacgg
    20341 taagatagaa attgtccctg tatttctaga caatgcatgc ataccgcccc acaaaaaagg
    20401 aatcgccaaa tcaggggctt ttattttaaa acgacgattt aacgaaaata attttacaag
    20461 aaattggcaa ccaaacaaaa cgattaccgg gtaaatatac caaaacaaga taagtggtgt
    20521 gaatgtgccc atgaacgtaa actccttttt tatcgttgct tcaagtatac tgtatatact
    20581 ttacacaact gtttcatttt atttaaattt atgcagttat attctatatc ttacaaacac
    20641 attccatgta ttccaattcc ctatccgtta taataaaaga aacaggcaac aggaggaaaa
    20701 tcatgatcaa atattttaca ttagataatg agaaactgcg taataccgga aaggaaactg
    20761 accaaacgat ctggtattgt gtagaacgtc ctaatcaaga agagattcat cagctgacac
    20821 atcaatttga aattccgtca gattacatca cttccatatt ggacgatgca gaaaattccc
    20881 gtacagaaga attcaatcaa tcaaagttga caaagccagc gttattattg ttacaatatc
    20941 cttttgctaa aacaagtcct agcggttatt ttcaagtgga tacatacccg ctctctatta
    21001 ttttgacgcc caaaaagaag cttatcacaa ttacgaatca tgaaccactg tttttaaaga
    21061 aagtctttag ccagaccttt ccaaaaagtg atatttcgcc taatctcaat atcttctttc
    21121 aattcatttg gcaggtcaca ctctcttaca acagttattt agctacgctt caatctcaat
    21181 gcgatacttt ggaagccaaa cttcaagtat ccactgaaaa caaacaactt tatcaaatca
    21241 tggatatcca aaaaagcctc gtttatttca gagaagcgac ttctgctaat ttagatgcat
    21301 tgaaagaact agcgcaaaat aaacttttcc aagaaaatca tgctttgaaa aatcatttac
    21361 gtgatgtatt ggtggaaaca gagcaggcaa tgactacttc tcgcattcaa ttaaaactag
    21421 tagaacaaat gaatcagact ttttcagcaa tcgtatcgaa taatctgaat aatattatga
    21481 aaattttaac ttctttaacg attattttaa ctataccgac aatcgtcggc agtatctatg
    21541 gaatgaacat taagttacct attgctgaaa gggatgatgc ctttatatgg ctagttctat
    21601 taacattatt tatttgtatc gtaactacct attatttaaa aaaagggaag tttttataaa
    21661 aaagccctct tgtggaaatg gaaaaaagat tgtacacttg aatcaactaa ttaatgacga
    21721 ggttgaaaac aatgcgtgaa aaaaaacaaa caagtacttt tgctaaagta acaaaatttg
    21781 tcatttggac aatgctgatt ttaacgattg gatctgttgt attgactgct gtaatgagtg
    21841 tcatgtaaaa ataaaatata gctatcagct gctgaaaagg acctgaacca ctgcgaggtg
    21901 gctcaggtcc tttttgcgag tttcaatagt tcttttgcat gttcgagtgt gagttttgta
    21961 atctcacttc cagccagcat acgtgcaatt tcttcttctc tttcttttgt tttcaactct
    22021 gaaacagaag tttgcgtacg tccatcaatc actgctttca caatataata ttgatagtcg
    22081 gcgactgcag caacttgagg cagatgagtg atgcacaaaa cttgagagta tttcgatatt
    22141 tgagaaattt tatctgcgat ggcctgtgca actcttccgc tcacacctgt atccacttca
    22201 tcaaaaataa tacttgtcac accttgctca gaagaaaaaa tagttttcaa agcaagcaac
    22261 atccgagaca gttcgccacc agaagcgact ttcaccaaag gcttcagtgg ctctcctgga
    22321 ttggtcgtaa tgtaaaattc gatttggtca aatccatcat gattcaattg tctattttct
    22381 tcttttaaga aacgtacctc aaactcagtg ttttccatat acagactttt caattcatgc
    22441 agaatggatt tttccaattc ttttgcgatt tttttacgcg cttcatgaag atctgcggct
    22501 tgttgctgaa gtagaagctc tttttgctct agttcctctt tcattttgtc caattgacct
    22561 tctgaaaaat cagaagaagc tagctcttcg gtgatttcgt catagtaagc caatattgac
    22621 tcaatggatt cgccgtattt acgtttcaac tgtcggatta attctaaacg ttgcgtaact
    22681 tcttctaaac gactctcatc taactccaat aaatcaatct gacgactcat atcgccgatt
    22741 gcatcttgca gcaaatagta agcactttgg atattttcat aaattgcttc atactccaca
    22801 tctagatggg cgatcccttg cagttctgaa acggccaagc caacaccatc tacactgttt
    22861 tgttcattat tcgtcagtgc tccgtaacct gcagctaatc catcagcaat tttttgataa
    22921 ttgctaagtt tttctctttc ttcttttaat ttctcttctt ctccgacttg caaatcagca
    22981 gcagctattt cttcttgctg gaaatggagc atatctattc tctgaacata ggatttttca
    23041 ttttgctgca cttttcgtac ccttttttcc agctcacgat aagagatata ttcttgctga
    23101 taactctttt tcttctgttg gaaagctaca tctccaaaac gatccaataa agcaagatgt
    23161 gattcaggct ggagcagttc ctgatgttcg ttttgtcctt ggatatctac taaataactg
    23221 ccgattcttc tcagattagc taatgtgata atatgaccat tcactcgaca aacatttttt
    23281 ccagttaagg acatatcccg tcgaacaatc agattgtcct catctgtttc gatccctaat
    23341 tcaaccatca gttcagagaa tccttcttgt tttggtagtt caaacaaccc ttcaagcaca
    23401 catttttctg ctccttgacg tatataatcg cttgaacctc ttcctcctgc taacagacct
    23461 aaagcatcga taataatgga tttacccgca ccagtctctc cggtcagcgc ggtcattcct
    23521 tcgtgaaaag aaagccgcaa ttccggaata atcgcaaagt tagtaatact gatttcttgt
    23581 agcatcacat tcgctcctca acttaaaggt aatgcaggaa gtctttcttc aataaaactg
    23641 cctggtcttc agttcgtgta atcatcaaaa cattatcgtc atcattgata atcgaaaata
    23701 attctttctt gtaacgttta tcaatcaaat ttgctgctgc cgaagcattt cccggcaatg
    23761 ttttcaaaat aacaaatttt tccatttggt caactgccac gaaagcgtct tttaatagtt
    23821 tttccaattt agtatttaca tcttcgtttg tttcagctgg tatgctataa cggtagccgc
    23881 catctgaagc cggtaccttg atcagtttca gttcctttat atctctggaa atcgtagctt
    23941 gagtaacaga tattcctttg cttttcagga tttcaacaaa ttcttcctgc tttcggatat
    24001 cttgctcact caacaatcga gtaatcaaac gatgccgatc tttttttctc attacgtctt
    24061 cgctccttag catattcttc ttaatcaatg atagcttatt tccttggtat aataaagaag
    24121 aatccgcata cttctcactt gattttcaca gttacaattt atttatggtc aaactgttcg
    24181 tgtgcatttg aaaccacttc tgccatatta atagctgaat tgattgttcc gctttcgggt
    24241 actttcctca agtgagccaa gaactcgata tttccttctc cccccgtgat cggagaataa
    24301 cttaattcca gcacatcata tccaccttgt gccgcaaatg acaaaatgtc ttctaaaact
    24361 ttttgatggg tctgagggtc acgaacaatt ccgtttttcc ctacagcttc tctgcctgct
    24421 tcgaattggg gtttgattaa agcaatcact tctccctctt cttttaaaat agcatgtaat
    24481 ggaggcagca tcagttttag tgaaataaaa gaaacatcga taacagcaac ttctggaatt
    24541 cctaaggtaa agtcttcggg ttttgcataa cgaaaattca ctctttccat tacgacgact
    24601 ctaggatctt gtcttatttt ccaagctagc tgattgtagc caacgtctaa agcataactc
    24661 atttttgcgc cattttgcaa agctgcatca gtaaatccgc cagtggatgc accaatatct
    24721 aacaatgttt ttccttcaac agatagatca aatgctttga gtgctttttc taattttaat
    24781 ccccctcgag aaacataagg aagtttttta cctttgactc ttaattcact actcactgga
    24841 attttttctc ccggtttatc aaaccgctcg tttttttcgt tgtaaatcaa accagccatc
    24901 actgaacgtt tggcttgttc tcttgtttca aataatcctt gtttgaccgc taatacatct
    24961 acgcgttctt tttccattct ttccctcatt tctccacatc aaattgcttg atcatctggc
    25021 gcatcagccc gccatcaaaa gaaaaaacgt cctcttctaa tttgtctatg atcttgttgg
    25081 cggataacag ctcaatctcc aacgcctcac gtgtcttttc taggcctaat aaacgcgggt
    25141 aagtattttt ttccatacgt tcatctttac cagctgtttt ccctaatgtt ttcgttgtac
    25201 caatcacatc gagtagatca tctcttatct gaaaagctaa acctaaatgt tcggctaatc
    25261 gttgaagtag caataagatt tcttcaggtt gcttcgctaa tatgccacca gccagcaagg
    25321 cataacgaat caaatgccct gtctttcttt catgaataaa tgctaattct tctaaagaaa
    25381 gctttttcga ttcgccctcg atatctgcag cttgcccagc aaccatacct tgtgatcccg
    25441 cacaaacagc taattgctga agcagcagaa gcttaggaga attacctaag tgtgccatac
    25501 tgattagctg gaatgctcct gtaagcaaac catctccggc caatatagct aatgcttcgc
    25561 cgaatacttt atgattggta ggttttcctc tacgaagatc atcattgtcc atcgcaggca
    25621 aatcatcgtg gatcaaggaa tacgtatgca ccatttctaa agcagctgct acttgataga
    25681 cgggaacatc aataggctca tcaaaggaag caacggccgt taaaagtaat aagggacgta
    25741 ttcttttccc gcctgctttc actgaataac tcatcgcatc atagagtcct ctatccgttg
    25801 tatgctcatc taaaaaagac aagatttctt tttctacagc aggtagatga atagctgaaa
    25861 agtcagttaa tctcatctca gctttcctcg tttccgtcaa aatcaacttc ttcatttgat
    25921 tctgtcatca ttttcgtcaa ggtctcttct gctttagcta atgtatcttg gcaatgtttg
    25981 cttaattcca ttcctcgttt aaacgaatct agagctgatt ctaatggaac gtctccttgt
    26041 tctaactgca tgacaatttt ttctaattct tgcagtgact cttcaaaagt tggattttcc
    26101 atatttttat tctcctttgt ctatctcttg tacttgtcct ttaacgattc catcttgata
    26161 atggatcgtc agcaaatccc cttgatgaat ctcggtgaca ctttttacta cttgatcatt
    26221 taacgtcgta tagctgtagc ctcttcccat gattttcaat ggactcaaca aatcaagaga
    26281 ttgaacagct tgagcaaaac gctgctgctg ttgtttcata tattgttgga ttttctcttc
    26341 taagcgtttt tctagataag cagtttcttg gcgagctgct ttaactcgat aaagcggagc
    26401 cacttgttcc aattggtggc ttaatgcgat cgtttgtttt tctttcgcat gataaatttg
    26461 ctgtgtagct tgatataatc gctgtttcaa ctggtctaat ttgatggtct gaccttcata
    26521 caatctttca ggctgccgaa atacatagga acgtcgagca cgctcgaacc gttcttgttt
    26581 gcgctggatc tggcgtacaa acgcctgctg caatctagct tgccgttctt gaatgcgcat
    26641 caactcttct gacaaaacag gaacagctaa ttctgcagct gctgttggag tggcagcccg
    26701 aacatctgct actaaatcag cgatcgtggt atctgtttca tgtcctacag atgaaatcac
    26761 cggcgtatta gcttgataaa tcgcccgagc aacccgctct tcattaaacg gccataaatc
    26821 ctcaatcgaa ccacccccac gaccgatgat cattgtatcg aaattgccca tctcttctac
    26881 tctctggata ttcctgacaa cattgtccgc tgcttggttt ccttgtacaa ctgttgggaa
    26941 caaaaccaat tggacaattg gatatcttct tttcaccgtc gtgatgatat cgcggataac
    27001 cgctccgctt ggactggtaa ggaccgcgat tcgctttggg aaacgtggca gcattttttt
    27061 aggcccttga aaaaggcctt ccttcatcag cttttctttt cgttcttcca attcttgata
    27121 taaagcacct attccatcag gctccatgtg ctctacatag atttgataag aacctcccgc
    27181 ttcatataat gaaatacgcc caacgacaag gactttcatt ccttcttttg gctggaatcg
    27241 taatttttgg aatgctcctt tgaacataat agcagatatt tttgcatggt catcttttaa
    27301 gctgaaatat tgatgggcat tggcacgtaa acggaaattc gatatttcgc ctgttaaata
    27361 tacgcgttct aaataaggat cggcatcaaa ttttcttttc aaatacttcg ttaatgtagt
    27421 gacagttaaa tattcttgtg ccatctattc acctgctcca aaagcttcgg cagattttac
    27481 ggtttgatac atcagcatcg tgatagtcat tggccctaca ccctttggca caggagtgat
    27541 ccagcctgca ataggctcca cttcatcaaa tttcacatca ccgatcagct taccgttagc
    27601 atcacgattc atccctacat ctatcactac agcacctggt tttacgaatt cttttgtgac
    27661 aaaatgtcca cgtcctattg ctacgactaa aatatctgct tcttgtgcca gttcagccag
    27721 attttcggtt ttagaatgag caacagtaac agttgcatct gccatcaaga gtagctgagc
    27781 catcggttta ccgacgatat tgcttcgtcc gataactact gctcgtttac ccgccggatc
    27841 gatagaataa gcttcaaaca ttttcatgat gccgtatgga gtacaaggaa tcttgtccgg
    27901 tttacccgca aggagtctgc ctaaattcat cggatgaaaa ccgtctacgt ctttttcagg
    27961 gcggatagct aacaggatct tttcttcatc tatatgcttt ggcaatggta attggaccaa
    28021 tataccatga aaactaggat cttgattgta tttttcaatt tctgcaagta gttcgtcttc
    28081 agaaatcgtc tcaggataac gctctaccaa tgaatggatt ccgatttctt ttgctacacg
    28141 ttctttattt cttacataga tttggctggc tggattttcc cctaccaata aaacgactaa
    28201 tccgggatgg ataccattat ctttcaattt ttggattttt tcagcaatct ctgcctgcat
    28261 tttttcagct aattcttttc cgttaatcaa ttctgccatc taaccactcc cagatatttt
    28321 ttgtgttttc attctaccat agaattttcc cgatttataa caggtttatg atgattcata
    28381 gaaaaaagct aggacttacg ccccagcttc taattctttc aatacattgg ataaaacacc
    28441 gttgacgaat ttacgtgaac ggtcatcact aaatgttttt gccaactcga ttgcttcatt
    28501 taaagcaaca gccgctggta catctgtcac ataagtcatt tcaaaaatcg cgatacgcaa
    28561 gatcaccaaa tccatttttg agatccgatt gatgtgccaa ttgtttttta aatgtttttc
    28621 aatcacttta tccaattcat ctttttttgc acagacaccg ccaactagcg tatcaagata
    28681 aacaggaaca aactctgatt catcttcgtt gatcatgtcg cggtggtcta attcgattgc
    28741 gttgaaaatc gcatcttctt ttgttaggtc tgcattgaag tctaatggaa ataaagcctg
    28801 caatgccatt tcccgaatct cgtgtcttga taattcttta ttcattctct tcctcttcgt
    28861 cttcaaaaag ttcttcgatt gttggttgtt ctaatttttc aggaacaaca gctactacat
    28921 ggatattgac ttcagataaa tcgatatccg tcatgaaaag gacttgctgg ctgacccgtt
    28981 cctgcatttc cattgctact ttaggaactg aaacaccata ttctagataa cagtaaagat
    29041 ctactttcaa gccatcttcc tcgtttgcta agtaaacacc ttttccgtga gctgcacgtc
    29101 ctaataattc agtgacattg ctggcaaaag ttccgcgcat tccgtatacg ccttctactt
    29161 tagaagctgc aatcccgata attacttcga tgacttcagg agcgatcacg atctctccta
    29221 aatcttggtt tgcgtttaaa atcaaatttt tttcatctgc catttattta ttcctccctt
    29281 tgcctaagca cgagatacat ccgaattctc tatgatgcgg catagtgatc acgtggtaag
    29341 aaaactgccg ccgatcagct gcaaactgct atgaattgga gaaaacaccc aactttatta
    29401 tctcttacag cttaaaagtt cttctatagt gtatcattaa atcgagtgat tttctataac
    29461 aaagtctgtg aacactgatt tttacgctta cttcttctat tgatgaaact ataagatgat
    29521 caattctttt ggtgaacgag tcaacacacg atttccatct tctgtgatca acaagtcatc
    29581 ctcaatacgt actccaccga ttcctggtaa gtagatccct ggttcatcag tgatcacatt
    29641 tcctggtaca aattgctttt ctgcacgaga agaaacgttt ggtccttcgt ggatctctaa
    29701 accgatccca tgtcctgtag aatgtccaaa tgcttccccg taaccatgtt ttgaaatata
    29761 atctctagca actgcatcta attggacacc agtcattcct ggttttgcag catcaataac
    29821 tgctaattga gcatctaaga ctatttgata aatttctttt aatttttccc caggatcacc
    29881 aatagcgaat gtacgtgtca tatcggaaac gtacccttcg taatagcaac caaagtccaa
    29941 tgtgatcaag tcaccttgtt cgatgatttt tttgcttgct acaccatgcg gcatagccga
    30001 acgcaatccg ctagcaacga tcgtatcaaa agaaacacct gaagcgccaa gagaacgcat
    30061 ataaaagtct agttgatttg cgacttcaat ctcagtcatg cccggttgga tcattttcag
    30121 gatgtgatca tatgccatat ccgcaatatg acatgctttt tcgataatag cgatttcttc
    30181 ttcatctttc acttctctga gttcttcgat catctgtgaa acaggtacta attccgcatc
    30241 gatgatttct tctaaaaccg aatattctaa aaatgagact gttgtttcct caaaagctaa
    30301 tgagtgcagt tcttctttgc gtactaagtt tgctacctca tcaaaaatag gtccgacatt
    30361 tttaataatt tcaaaccctt gagcctgcgc tgccgcctgc tcggtataac gaaaatcagt
    30421 aataaaaaat gctttttcca aagtaataac agctaatcct gtcgtacctg taaagttcgt
    30481 taagtaacga agattgtacg gactggtaac caaaaatgaa tccaaatttt cttcttgcat
    30541 ttttttgcgc aatttttcta cacgtagcat cataatgaaa ctccctcttt ctttgcacgc
    30601 ttttttccat tatagcaaac ttttcttcgt acacgacagt taaataaaaa tttccggaaa
    30661 acttcttttt attttaaata aataaagcca aaaaggagtc acagtctcgc tgcaactcct
    30721 tttagtttac tattttgtaa aaacttatga tcgttttcca aagaaaaatg agacaactgc
    30781 taccaaaatc actgcgccta aaatagatgg aataagtgcc atacctgcag cttttggtcc
    30841 ccattcgccg aaaatcgctt gtccaatact tgaaccgact agtccagcaa tgatattcgt
    30901 tacacagccc atcgaatttc ctttactggt gatcgcacct gcaattgctc cgatcacggc
    30961 tccaacaatc aatgtccata accaactcat aatataacct ccttctttgc taatcaattt
    31021 tagcatatgg gatttaaagg ggacaaataa ttggttttgc tatttcatat tttcttctgc
    31081 aaagtaaaaa aataaagaaa acctcctctt agaagttttc tttatttttt acttgcgttt
    31141 aaacatacta cgaataccac gtttcactac atcaaagaaa ctcaatgatt gtaccattct
    31201 atcttcatct acatattctc tgatcgcacg aagcactttt ttaggttctt tggaagaaaa
    31261 tgtgtatgta ccattttttt tcgtttgaat ggcatatcga ggaatccatt ttccctttag
    31321 catgactgat gctagtacat aatccacttc ttcccatgga atttggatat atttgcgtct
    31381 atctcgcgca tggtaaaact caaaaccatt atcacctatc atgatttgcc cgtaatctgt
    31441 cagacccgta aacgaagtag catcaattac caagtctact tttgtattga ttgattgaac
    31501 cattttctcc ctccagatat ttgtttatga acagtatact atataaaaaa agctcgggac
    31561 aacgcccgag ctatattttt atggattata aaagtccgat gacgtgtcca acaacaccga
    31621 ctacgaataa accaaggatg ataacgattg gagaaacctt tttcttaagc aaccacatac
    31681 ataacaatgt taatcctaat gcagctaaac caggaatcaa ttggtctaag ttgtcttgta
    31741 atgttgttac cttttcagga gaaagggcta agccattatt aacttgttca aatgcactct
    31801 tgattccttc accacctgaa ggcaatttat cccattcgat gtaagcacct ttatctaatt
    31861 ttacagttga tacaactggt aagaatttga ttgataccca tctttgtaca agggcagcta
    31921 atacgaacat ccctaaaatt gaagcacctt ttgttacgtc ttgtaacaat ccgccagaaa
    31981 ggtcatcagt aattttagaa ccagctttgt agccaaattc ttgtgtatac cacatgaatg
    32041 cccaacgaat aatgttccaa cctaagaaga agatgattgg tccaaggata tttcctccca
    32101 tagctaatga agcacctagt gcacctagca ttggacgtac tgtaaaccag aacactgggt
    32161 caccaacacc agctaaagga cccatcatac ctactttaac cccttgaatc gctacatcat
    32221 caacaggcgc gccattcgca cgttcttctt ccagagccaa tgtaacccct aaaattggag
    32281 aagcaatgta tgggtgtgta ttaaagaatt ccaagtgacg ttttaatgct gcagaacgtt
    32341 cttcttttgt tttgtataat tttttgattg ctggaatcat agaaaatgcc cagccaccgt
    32401 tttgcatacg ttcgtagttc catgaacctt gaatgaaggt agagcgccac gcaacagcta
    32461 aacgatcttt ttttgataat ttgatttttt cttctgccat tttattttcc tccttcaaga
    32521 ttaatagtca ttcaaaatgt cgcctaaagg gtcaccagag ttgcttccgc ctccattatt
    32581 tgatgaacca cccattttag aaaggttcaa gtaaataaga gctaatgcaa cacctaaagc
    32641 acctagagca atcaatgtta attgagaaat tgcagctaca acgaaaccaa taacgaagaa
    32701 tggccatact tcttttgtag ccatcatgtt gattaccaaa gcgtaaccta cagctacgac
    32761 cattccccca ccgatagcca taccatctgt taaccatgct ggcattgatt caaggaatga
    32821 ttgcacagtt tgtgctggga taaatagcaa cgctgctgct gggaccgcga tacgaacccc
    32881 ttgtaaacat acagcaatga tgtgccacat ttcaacctta cggatgtttc cttcttcagc
    32941 agccgcatcc atcaaatgaa cgattggaac agcgattgta cgtacgatca ttgttaagaa
    33001 caaccctgca acagccaaag gaacggcaat cgcgatcgca gaaggaacac ctttaacacc
    33061 ttgtccacct aaaactaaaa taattgctga tgctactgat gctaatgcag catctggtgc
    33121 tacagcggca ccgatatttg cccaacctaa agcgatcatc tgaagcgtac cgccaagtac
    33181 gataccagct tcgaggttac cggtaactaa accgatcaat gtacatgcca ctagtggttg
    33241 atgaaattga aattcatcaa gaataccttc cataccagct agaaaggcaa ctaatacgac
    33301 taaaattatt gaaataatag acatgataag cctcctataa attttaatat tatttattac
    33361 gcttttgcta attcagcttt tgcttttttc aaaatatcat ccatgttatc gcgtgaatca
    33421 ttaggtactt tacgtacatc gaatttcaca cctttttgtt caagcttctc aaacgcttca
    33481 acgtcttctt gtcccataga caatacttta cttacaacga ctttaccgac cgaatgcgcc
    33541 attgacccaa catttaattc tttgatgtct acgccacctt caacagctgt caatacgtct
    33601 tcaggatttt caaataaaag caatgctttg gtatttccaa aacgagggtc ttttgctact
    33661 tcgatcattt tgctaatagg aataacattt gcttttacac ctggaggagc agcttgttcg
    33721 attagtctct tacgcagatc atcctttgat actgcatctg aaacaacgat aatacggttt
    33781 ggttgcactg cttttgtcca agctgtcgca acttgtccat gtaacagacg tgagtcaaca
    33841 cgtgctaata catatttgat ttttccgtct ccgaccacag ttccttctgg aagtgctcct
    33901 ttaggttgtg cgtcttcaac tgctgctttc ggtgcttctg ccggttctaa ttcttccggt
    33961 tttactttta caccgtcttt tgctgtttca atgatgtgtg ctgcaatttc atgagcagat
    34021 tccattgaaa agcgtgatgc gtaggcttca atcaacattg gtaaattcaa accgctgacg
    34081 attgcccatt tatctttgtg ctcttcgaat aatgtgtttg attgattgaa cggtgttccg
    34141 ccccataaat caactaagaa taacacttcg tcttgattat caaaagaagc aaccgcttct
    34201 tccaatttgg ctctcaagtc atctggacct tcacttggtt tcaaaatcac ggctttgacg
    34261 ttttcttgtt cgccaaagat cattgaaccg gattgtaaga ttccttcagc aaattgtcca
    34321 tgacttgcta ggataatccc taccatattt atacctccta tttacttttg ttgtaacaaa
    34381 ttcaaaaata ttaataattg ctaaccatta caacagaatt atttgacctt ttccaacaac
    34441 cattcttgct acaccttttt gattttcgct agtagttcca tcaaaaggat tttcctgtca
    34501 gcaggtactt ttcgaatctc taactccact tcttgactgt tcaagtaacg aaaggaatca
    34561 atatcttgtt gatttactga aacaaaattg gtaatcattg atttgccttc agtaaaactc
    34621 ataccgccga tattcaacga cttcaactgt acaccgcagc ggacaaccgc ttcaacatct
    34681 tggggattgg tgaataaaag catcactttt gctcgtgata ataatccact atgatagatt
    34741 tgaatcattt tctgcacggt tattacattt gctgtgatcc ccggcggtgc agcttctttt
    34801 agtagaaact ttctcaattt atcttgagcg actacatcgc tgatcacaat gatacgttca
    34861 acacctgttt gcttcgacca aactgttgcg acttgcccgt ggattaaacg atcatctata
    34921 cgtgccaatc gaatatccat ctgcaaaact ccttcttcct cactacttca cagaaacccc
    34981 tatcatttct gtgtgaacaa ccacctccta gcacacacta ctataagcaa aaagcgtgcc
    35041 aactttttta cgatacacta attgaattta aaggattctt aaccaataca caaaaacgat
    35101 acactataaa agtgtatcgt ttttgtgtat tcatggcaca gtgtattctt ttttatctaa
    35161 ctgattatgg actagacgta gcagataata catttcttct atcggcactt ttacttttat
    35221 ctgatcttcc aacagaacaa ctgaggctgc caattggcta taaagaggat cattcgttgt
    35281 atttgtcaat tcgttttctt ccacagtcaa tggttcattc cgaataatac gttcgatcat
    35341 tcctgctgtg tgcatcacta ggttgatttt gaatccatat tcttcatcgc cgattcccgt
    35401 cgtctgagag atttgtgtag aaaattgcca cattggatcg attaacttac taccattaat
    35461 aaagatgaag ttatcagaga taaactcaac acacgtattt ttagctgact cttcatctag
    35521 ggagatgaat tcagtttcct ccaattctga ttccatcagc aactgatcca aaatgacttc
    35581 gatattttga tcaataaaac gttctaaagg aataaatggg gcttggatat tcaaatcagt
    35641 aatcccagta gtagctatga tctgatattc tttttggata actggcagtt cttctttcac
    35701 ttccacaatc gacaaagcta gaacttctaa ttctgtttct tgacgcttgt ttaccgctcg
    35761 ttcgatcaat tctttgattc tttgtgctgt accttcgcca gatgcgcaga tagccaaaac
    35821 agcttttttc ttatgaatag gttttgcttc ctgaacagat tctacagaag atatttctgc
    35881 gtaacctcga aaatttttca atgattcatg caacatatct agatctgtcc ctaatactga
    35941 gacctttcgt acagtttcca aaactagaga agttgttacc atatcaacag tacgtactgg
    36001 aattccagtg tctctttgaa tttgcgaatt aaacgacgca agggacccca tatctactaa
    36061 gagtatgacc ccgcttcctt catctacttc ttgtacattg cgttcaattc ggtccaatgc
    36121 tgttttcgga tccatatcca aaggcatgtc tactgctcgt acgttctctg catctaacaa
    36181 ctgttggaca acttggacca tactgcttgc tgtgctgttt ccgtgagctg caacgactac
    36241 accgattctg ccgcttgctt gatttgcccg taacgagacg agtagaacgg tcaagtaata
    36301 gtcttcactt tctggaatct ccacttgaaa atacgtttcg atataactac gcacttcttt
    36361 cgcaacagca tattcatctg gaaaatcaaa agccatctca cggatgttat cattcgtatg
    36421 ccgttcttct cctaagttga tttttttcaa aaaagaactg atatgcaagc tcatagcata
    36481 aataaagttt tgctgaaact catatggtaa agatcttttg accattacat aaatctgatt
    36541 ggtcacttct atgattttcg gatctacaaa ttctgctaat ttgctgtctg cattaaaaga
    36601 aaaaccatgg tctttataga aagacttcag atggatgttg atatctgtag aaatgaaatg
    36661 attgattgct tcttgatcca aaccatccga cttcagtaaa gctgctttat ctccgatgat
    36721 atcatataga ttataaggta actcatagga gtccgattgg attttcatga tcgtatcatt
    36781 tggtgctaca gttatctttg gctccaatat tttagaaagc tctgccattg cactgcggtt
    36841 gcttgctaat tgaatcaatc cgctacgaat cccttctgtc aaatcatcga ttgtgatcga
    36901 gatttcgggt gattgcatat gatttagaaa tcctcgtgca catactaact ggatatttga
    36961 cttcaattgt cctatatttc cataggtcac actgccgatc aatgcttttg ctacatcttc
    37021 tgtcaaagaa atctttcgct gcgtgcggtt tgcttcgtgg gcaatcatga tttttactaa
    37081 atcgactttc tcatttgcag gacgtttttc aaaagccgga agtttgatat tgattgggat
    37141 acgccgaaca aaagtttcta atagagaaga acctgggtct tctgtggtcg cacctacgat
    37201 ccgtacattt gcttcatggg atttcgttgt ttccccaaga cgactgtaag ttccatgatc
    37261 cataaagtaa aagatcattt cttgaccttc aggcggcaac cggtgaatct catccaaaaa
    37321 cagcataccg ccatccgctt gatcgataat ccccattttt tcttcatctg cgcctgtaaa
    37381 agcacctttt gcgtacccaa ataaatgact catcaaaagc tcagggttat tggcataatc
    37441 agcacaattg aaaacaatca gttccgtttc ctcatctatt acatgatgcg cttttgcaaa
    37501 atgatacatc gcgtgtgcaa aataagtctt tcctgatccg gtaggtcctg taatcaaaca
    37561 atttaatcct cttggcggat acaaaatcgc agcttttgcc tgttccactg aatttttcat
    37621 gctgccgtta gctccaatga tttttgcaaa gatatcctta gtatctattt ggagtgtatt
    37681 tttttcttca gagaccggcg tttctttgta actgactaca tgttttgtaa ctggctttac
    37741 gtgcgtaacg agtgtttgat atatgtaacg tactggtcgt ccgtctgtct ttatcagttt
    37801 tccttcgcgc actagctgat tcagatcttt actgctattt gttcgctgaa tccctaacac
    37861 ttcagcaatt tcctgtgttg ttactccttg atcatattct gcctttgtta aatgagcggt
    37921 tttttctttg acatacatat aaattcggtc aatgcgtttt gccaatggta tcgctccttt
    37981 tctatagact attgtactta ggcttcaaaa cataatacta tgtatttatt tctgtttcgt
    38041 caactattta cctgattttg ttttgttgat aacacgcgga tagatagaaa tgttcaattt
    38101 aaaagaacga aaaatcccga tacgattttt ataatggaaa caagaaaaag aacactgttt
    38161 accatataat tatttcactg atagcaaaca atatagcttc catcaattga aaacgatagt
    38221 tatcaaaaca tcctgattcc aaattggcaa aaaaatatta agtgtaaaaa gacaaagtaa
    38281 acttcgtagc ctccactaca acttttattt ttataaacaa aaaaggctgt gacaaaagtc
    38341 ttgtcacaac ctctttaatc ataagattca attaagcttc ttgagctact ggataaacag
    38401 acacttgttt tttgttacgg cctttacgtt cgaaacgtac tacgccatca acttttgcaa
    38461 ataaagtgtc gtctccaccg atacccacgt ttacacctgg atagattttt gtaccgcgtt
    38521 gacggtaaag aattgatcca cctgtaactg tttgtccatc agcacgttta gcacctagac
    38581 gttttgattc tgagtcacgg ccgttagatg tagaaccgcc accttttttg tgggcaaata
    38641 attgtagatt catagataac aacatggtct gcacctccta tttatcatta attgttttaa
    38701 tttggatata ttctgagttt tcagcttcta tcgattgtaa tcctaataaa agattttcta
    38761 atagaatctc agcaatattg ttttgttctt gtgtcattcc tgaagtgact tcggtataaa
    38821 gataaccgcc ttcttcttca ttcatttcaa caatcggctc aaaaccggct aatgaggcaa
    38881 tcccgttgac agtgctgatc gctaacgcag aaacacccgc acaaacgata tcacttccat
    38941 aaggaccagc atcagcatgc ccggtcaaag tgaatgaaac gatctgacct gcgtcatttc
    39001 gcttaaatgt tcctttgatc atatataggc cttctttctc aaacgctatt aagcgttgat
    39061 cgcgtcgata accacttttg tatatggttg acgatgacct tgtttacggt gtgaatgttt
    39121 tttaggtttg tatttgaaag taacgacttt cttttgtttg ccgtgttttt caacagtacc
    39181 ttcaacagtt gcacctgcaa cagttggagc gcctactttt gtagattctc cgcctactaa
    39241 gataacttcg tcaaaaacaa ctttttctcc tgcttcaacg tcaagttttt caacatagat
    39301 tgcttgtccc acttcaactt ttacttgttt accaccagtt ttaataattg cgtacatgct
    39361 ataaagcacc tccttctgat atacttagac tcgccatccg aagtgacagc atatgctgtg
    39421 cttattactt gttctgagcg gttgtagctg tggagtccac aattacaaca gtactagctt
    39481 accagaacag caaccgttag tcaactaaat ttaaaatcaa aaagtaaaac aaaaattcaa
    39541 gcaatacttc tgcttttcta tcctcacagt tctaccaaat aaaatagtcg cattgtgttt
    39601 cttttattct tataataaaa gaaaaaggag gagcataaat ggaataccca aaagaactga
    39661 tacaagaagc ttctttaaga atgaacggcc gtcctgtaga aggatttatc gctgggcaag
    39721 gacctcttca tcctaaactg atgctcgtgg gagaagcccc agggaaaaca gagattgata
    39781 cgcatgttcc ttttagcggt caagctggaa aagaattgat gcaggcactt tcttcaactg
    39841 gcttaacacg agaagaggtg tatatcacca gtgctgtaag gagccgcccg tatcgcgtga
    39901 ctcatcggat caataaacgg acacaacagt cagaaactgt ttatccaaat cggacaccta
    39961 ctcgttccga agtatttgcg catgcaccaa tattggatta cgaactgatg aaggtccagc
    40021 caaaattaat tgcgacactt ggaaacattg gtcttcaacg tttacttggc aaagaatata
    40081 cggttaccaa aaatcatggt caactctata cagggcctgt cttacaatta acagaacaaa
    40141 aggatgccta cgaatggtca gaaaaaaact atcgaattct tccacttttt catccagcag
    40201 ctattttcta caatcgctcc cttgtacctg ttatccatca agactggcaa gtactagctt
    40261 cacttttaca gcaatattaa aaagaacaga caagaccata aaatcttcag tttacagtct
    40321 tgtctgttct ttttcattgt tttaatggct aattgcagta tatttcgcat gtaaagctgt
    40381 caacgcatga gcaactgttt gatgttgatg cgtgaatgta tgttcccaca agtgagaaag
    40441 ttgtttttct acttctggct gcaaggaagt atagataatc gtttgtcctc tttgctctgc
    40501 tttttcttga aattcatcaa aaactgcacg tgccgtcaaa tcaatacttg gtacgcctcg
    40561 catggagaaa atgacaccat cgtgatgatt tagttcttcc aatttatttt tgagtttttc
    40621 tgaagacata aaaaacagtg gaccactaat atacactact gtccagttat tcagcttttc
    40681 cgtttctggc agccccattc tttgccaatc gatcgtttct aatgaaatcg tgatagcagc
    40741 gcttttgaca acaaaaaata cacagccgcc gatcaccccg atcacgatag caatacttaa
    40801 atcaaagata actgtacaaa tcatcgtaag gataaacaat agaactgctg accaatattt
    40861 attagtaaat agctccttga tcgtttccca ttcattcata cgccaagccg tcacaatcaa
    40921 aacacctgca agtgctggca ttggaatctg tgacatcact ggcgcaaaaa caagcatgga
    40981 gagcagtaag acaactgcat gtatcatacc agctaatcta gtttgtgcac cagatttgat
    41041 ggccacgctt gtacgggcga tggccgcagt agcaggaatc ccgccaaaga aaggaagcaa
    41101 cagatttcca attccttgcg ccattaattc ttgattgcta tctaagggct tatttgccat
    41161 tcggccagcc gaagcaccac atagcaagct ttcaatcatt cctaataaag caatgctaat
    41221 agctggaact gccacttgtt gcaaggcaga aaagctaaaa tctcccatat tcaaacgatt
    41281 actactgatc aatgtttgtg gaatcttacc cacagttgct actggtaaat ggaacaatag
    41341 cataactgct gttgccaaaa tgatagccag cagcgaacta ggcattcgtt tcgcccattt
    41401 ttttggatag ataaacatcc ctaaaatgac tagccctccc atgatcaaag aggctgtgtt
    41461 tatcggaaat cctatcgttt gataactact tagtttttca ataacgtttg taccttccga
    41521 gtgaacacca aataaattat cgatctgacc taatgcaata atgatagcaa tacctgaagt
    41581 aaaccccgta attacgggtg ctgggataaa agaagtcagt gttcctaatc ggaaaagtcc
    41641 tgctgcaagt aaaaatagac ccgctaaaaa agtagcaagc aatacacctt gcattccctt
    41701 tgttgccgca atagacatca agatagctgc catcgcacct gtcggacctg atatttgata
    41761 aaaaccgcct gataaaccgc caataacgat accggcaatg atagccgtaa tcaaacctgc
    41821 ggccgcatca gctccgcttg ataccccaaa agccaaggct aaaggcaatg ctacagcagc
    41881 tactgtgatc ccagccaaaa gatctttctg aaatcttgaa agatcataac ctgaaaattc
    41941 tttttttagt gaggaaacat aatttttgaa cacctatcta actccttttt catgaaattt
    42001 t
//
LOCUS       DAKBVG010000005        38214 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000005 DAKBVG010000000
VERSION     DAKBVG010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 38214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38214
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.005"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(451..1761)
                     /locus_tag="OUD09_000227"
     CDS             complement(451..1761)
                     /locus_tag="OUD09_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HCT9691539.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1773..3191)
                     /locus_tag="OUD09_000228"
     CDS             complement(1773..3191)
                     /locus_tag="OUD09_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCT9691540.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3431..4999)
                     /locus_tag="OUD09_000229"
     CDS             complement(3431..4999)
                     /locus_tag="OUD09_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HCT9691541.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(5003..5413)
                     /locus_tag="OUD09_000230"
     CDS             complement(5003..5413)
                     /locus_tag="OUD09_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HCT9691542.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5410..6333)
                     /locus_tag="OUD09_000231"
     CDS             complement(5410..6333)
                     /locus_tag="OUD09_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HCT9691543.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6330..7208)
                     /locus_tag="OUD09_000232"
     CDS             complement(6330..7208)
                     /locus_tag="OUD09_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HCT9691544.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7195..8532)
                     /locus_tag="OUD09_000233"
     CDS             complement(7195..8532)
                     /locus_tag="OUD09_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HCT9691545.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8535..9638)
                     /locus_tag="OUD09_000234"
     CDS             complement(8535..9638)
                     /locus_tag="OUD09_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HCT9691546.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9712..11220)
                     /locus_tag="OUD09_000235"
     CDS             complement(9712..11220)
                     /locus_tag="OUD09_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCT9691547.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11186..11878)
                     /locus_tag="OUD09_000236"
     CDS             complement(11186..11878)
                     /locus_tag="OUD09_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HCT9691548.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11900..12874)
                     /locus_tag="OUD09_000237"
     CDS             complement(11900..12874)
                     /locus_tag="OUD09_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCT9691549.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            13174..14046
                     /locus_tag="OUD09_000238"
     CDS             13174..14046
                     /locus_tag="OUD09_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCT9691550.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14504..16702)
                     /locus_tag="OUD09_000239"
     CDS             complement(14504..16702)
                     /locus_tag="OUD09_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCT9691551.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16934..17926
                     /locus_tag="OUD09_000240"
     CDS             16934..17926
                     /locus_tag="OUD09_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCT9691552.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17986..19407
                     /locus_tag="OUD09_000241"
     CDS             17986..19407
                     /locus_tag="OUD09_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HCT9691553.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19400..20899
                     /locus_tag="OUD09_000242"
     CDS             19400..20899
                     /locus_tag="OUD09_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HCT9691554.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20938..22332
                     /gene="uxaC"
                     /locus_tag="OUD09_000243"
     CDS             20938..22332
                     /gene="uxaC"
                     /locus_tag="OUD09_000243"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCT9691555.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22491..23174
                     /locus_tag="OUD09_000244"
     CDS             22491..23174
                     /locus_tag="OUD09_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HCT9691556.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            23176..24012
                     /locus_tag="OUD09_000245"
     CDS             23176..24012
                     /locus_tag="OUD09_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCT9691557.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            24023..25777
                     /locus_tag="OUD09_000246"
     CDS             24023..25777
                     /locus_tag="OUD09_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT9691558.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(26053..26394)
                     /locus_tag="OUD09_000247"
     CDS             complement(26053..26394)
                     /locus_tag="OUD09_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HCT9691559.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26462..28633)
                     /locus_tag="OUD09_000248"
     CDS             complement(26462..28633)
                     /locus_tag="OUD09_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HCT9691560.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28802..29701
                     /locus_tag="OUD09_000249"
     CDS             28802..29701
                     /locus_tag="OUD09_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCT9691561.1"
                     /translation="MVQLVCDCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            complement(29772..30629)
                     /locus_tag="OUD09_000250"
     CDS             complement(29772..30629)
                     /locus_tag="OUD09_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCT9691562.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(30614..33316)
                     /locus_tag="OUD09_000251"
     CDS             complement(30614..33316)
                     /locus_tag="OUD09_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HCT9691563.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(33329..34618)
                     /locus_tag="OUD09_000252"
     CDS             complement(33329..34618)
                     /locus_tag="OUD09_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HCT9691564.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            34768..35814
                     /locus_tag="OUD09_000253"
     CDS             34768..35814
                     /locus_tag="OUD09_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCT9691565.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            35816..37969
                     /locus_tag="OUD09_000254"
     CDS             35816..37969
                     /locus_tag="OUD09_000254"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HCT9691566.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT        11274 a         7859 c         6793 g        12288 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcgag agtgtttccc gattcatttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa acgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt taacgactta
      301 gttatcttat cacgtctaaa aacatgtgtc aactcttttt tggaattttc ataaaatgtt
      361 tcaatgaagc gcgacaacgt tgtttaatat atcattaaaa aaaataaagg tcaagctaaa
      421 atattattta agcttgacct tattataatt tcaacgattg tcaaaagtat tgtttgcagc
      481 tgtgttgcca gtttctatag ataaatcaca gcttttgaaa cataagtttt gtactgcttc
      541 gattcgcccg aattcttttg cagttatttg gcaatgagaa aagcagacat cttctatagg
      601 cttttcagga aatgctacta aatcaaaagc tcgggaagga agtgagtaac ctggagaaag
      661 gcatgcctgt acgtttttga tgtgtatgtc tttgactttc gtcatttgct ttgattcagg
      721 tatttcttca gccactgctt gccaactttc tggcatttga gcaaggtcat tgctattttt
      781 tctattgtat tcattatgcc agtccattat ccaagaaaaa ggaaattgta catcaatcat
      841 attcagattt tctaccctga tattttcgat cactccccct ctttcttttg aagatttcat
      901 tcgaatacca cagtctgact gaaaaaaatc gatatcgtga atatatacgt cactaactcc
      961 agcactaact tcgctaccaa tagttacacc gtatcctgaa cggattttac atctagcaac
     1021 ctcgattctt gatgattttc gattgactct tgcaccattg ccatctctgc cagacttaat
     1081 agcaatacaa tcatcaccac aggccaaatc acattcatag acacgcacat ttgttgaaga
     1141 atcaatatca atcccatctg tacttggtcc atcattgttg cggatgatta ctttttctac
     1201 taatacatcg ttagaataag tgatttgcaa gttccaaaaa cctgatttct gaaaaatcaa
     1261 atcacgaaca acaacatgtt ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc
     1321 ataatccgca atccaacgaa gtcccttttg atcgtattca gctcttgtcc cgcttttttg
     1381 atcttttccc caatacagct cccaccaatg gtctccttga ccgtcgatta ttccttttcc
     1441 ttctataaga atatctttcg ccgagagaac attcaaaatc gctgctggcc atttcatttc
     1501 tacgccggct actcgtgtat cgatttctgg aaatgcttcg atatctgttc tccccttgag
     1561 gactgcacct tcttcaaaga taagatgact cttatctttt aaaaaaaggg ctccgactaa
     1621 ataaatgcct tttggaataa atacaggtaa gtgttctttc gcaccttgat cgattgcttt
     1681 ttgcagtatt attgtatcat cttgttgagt gtcgtttgtt ttaaagttca aagcattgat
     1741 aatccctttt tttgtccgca atacgtttct ccctatcttt cattgatttt tttcaattct
     1801 tcaccaataa atacagtttt tgaacgattc agtgggtaac ctactagcaa aatgattgcg
     1861 gatacaaata agcagaatgc tggtaacaag tttgccatcg tataaatcct ttcttgtact
     1921 aaaactgatt ggatggctcc acctgttgta gacgattggt aaccaatgag tgccagcata
     1981 aatccgccta ccgctcctgc acatgcttgt cctacttttc tcgcaaaaga atttactcca
     2041 tatactgttc catcttctct taagcctgtc ttatattgat gataatcaat cacgtcagta
     2101 atgaatgccc agaccatcaa attgaataaa ccagatccta aagtagcgat aaataaaaga
     2161 atcagatata cccatgcatt tgttatatga aggaaataca tcagcaagta catacatgaa
     2221 gaagcaagta acgcaaatac gcttgcttcc tttttaccaa ataattttgt cagctttgta
     2281 gcaaatggtg ctaaaactac tactgttccg taagtaaata aaagagcaac agacattgct
     2341 tgtttattat ggaagtaatc attgaataaa taagtcatat tcgtcccagc taaaatctga
     2401 ttgatcacaa tgaaaatgtc aacaatgact aaaataatca gtgctcgatt agctaacaaa
     2461 ccttttacca actgagaagc agatactttt tcgtttttga caactcttac tctttcagta
     2521 gtcaatttac aagttaacat atatgcgaca aatgctattg cagcacatcc gattgaaatc
     2581 caaaagaaat gattccctga aacgacttgt tgtccactct tgttatgggt atacattaaa
     2641 atcggaatga agaatcctgt agttgatccg ccaatcgcag aaccaatgct tcggaacgtg
     2701 gacaacgagg tgcggtcatt aggatttgag ctaattgctg ctgccattgc tccatacggg
     2761 atattgattg ttgagagaaa aatcccataa acaatgtatg aaacaaaaat ataagcaatc
     2821 ttcaccgtca ttggaagatg ttggacgaat ggcaaaaaca aaatgacagt cactaagcaa
     2881 aatggatact tcatttttct gatccaagga ataaatcggc cagtttctgt taactctgag
     2941 acatcacata atcttccaac catcacatca gcaaacgcat ccaaaattct cgaaatcaag
     3001 aataacgtac ctacgattgc ccctgaaacg ccaagaacat tcgtataata aatcatcaag
     3061 aaactattga ctaaacctaa aataaaacag ttacctagat caccgaacat gtagcctatc
     3121 ttatcggaaa tcccaaattc ctttttttct ttttttattg tatttgctgt aacagcattt
     3181 tgtgaaagca tttgaattcc tcctacctct tctagtaaac gttttcaaaa ttataactct
     3241 ttttttcttg tttttatatt cacaatgttg gctttttatt gcattatctt gacagataaa
     3301 aaacaaaaaa taatctaaaa acattgatgc ataagattga taactttttt tacataacaa
     3361 tatatacaaa caaaaaaatt cactaactag tgaaaataag ttagtgaacc tcatttatta
     3421 aagaattaaa ttacagatga tcttttccat catacttgaa aaactcaaaa tcagcataac
     3481 ttcctctttt tttatttaga tcatgcactg ctatcccgat aaaatttcca gtaaaccctc
     3541 ctgagagtat acacatgttt ttttgcggac ctactttctt ccattcttca gttgatacgt
     3601 ctttcaaatg aaactgtgct gtctctttct ttacagtgac tttcaaaaaa gtcttctttt
     3661 ctgtgagtgg aatcgtccaa tcttctattt gtgctactcc gttttctgat tctatcatac
     3721 gtaggcattt tcccttgtcc tcattccaag taacataaca ataaatataa ttggattcat
     3781 ttaagtacag taccagtcca gccatctgat taaaattatt aggcatgtat tcaaatgctg
     3841 tctccgcatc aaaagtaaaa tccgtttgcc gaatagctaa caaatgatgt tcgaaggtag
     3901 attgaagaga ttcaccggaa atcagacgta aaaaacctgg tctacatttt gtatcacacc
     3961 aatcagcatt tggcaaaatg cgtctggcat tccagttctt ctttagctct ccttcaaatg
     4021 tatccataaa ctgatgatct ttttcttgtt gtacctcttc atttgtctca atcattattt
     4081 cttccatcgg agttgtctgg ctatctttaa gtcttaacca atcgtcggac gtccaataca
     4141 cttcttgaat agcggtttct cttcccaaaa tagccgcacc gtttaatggt ctggtacaaa
     4201 gatatgtcat gtaccatctt cctgacgggg tttgtactaa gctcccatgt cctgtacatt
     4261 gcaatgtaga agcaggtttg tcactagctg tcagcatagg aaaaccagga tcaagctcgt
     4321 aaggaccagt aatattcttt gctcggcaaa ctgtaactga atgtcctctt cctgtaccgc
     4381 cttctgccgt catcaaataa taatacccgt tacgaaaata caaatgtggt gcctccgttt
     4441 tcgctaattc tgtacctgca aatattttat agatgggacc atcaagcttc ttcttctctg
     4501 catcatattc ttgtaatacg ataccgctgg atttattcgg tgtgtccaga cgataatccc
     4561 atagttcatt gacaagccat ttttttcccg tttcaagatc atggaacaaa gaaggatcaa
     4621 agccgttact atttaaataa attggttcgg accaaggacc tgtgatctca gatgcagtga
     4681 tcaaataatt gtggacatct ttaaacggac ggcctgcact tttgacatct gtgtaaacaa
     4741 gataaaacaa tccatcagca taactgatct gtggcgccca tatacttcca tttgtaggat
     4801 ttccttgcaa agatacttga gtggtcaaaa tatccgttgt atgtgaccag ttgactaaat
     4861 ttttcgactc atatactcgg attcctggaa gccattcaaa agaggagaca ataatgtaat
     4921 accgctcttt cactcttaat atattaggat ctggattgaa tcctcttaaa ataggatttt
     4981 tcactttgat agtctgcatg attcaggctc cttttctcat tcttactcga tataaactga
     5041 atgccccagc tgcgaaataa aaaaatgcaa aaggaaacgc aataaactga gtgccaatca
     5101 acaatatgaa tcctgttata aaatacaaac cagtggcagt tgttaagtat tttcctttag
     5161 cagtcaaaaa caatgctact gctaaaaaag caagtgaaat aaacaacaca acggcaaaca
     5221 gaccagtcat gtcctgtaat aatccaaatg tattttctaa agacaaagtt gaatgatcta
     5281 cagcaagcga ctgataactt tttttaaact ctgacgcaga catattactt aaagtaactg
     5341 ctaacccgcc aaagaaaatc aaagatagac tgccaccgac cattcctagc aatagttccc
     5401 attttctttt catttctgaa ccccctgctt ttctaaatgt atccattcag gtcttttgat
     5461 cgatgtttca taattgttat tacgttcaga gaatttgaca gtctgttcat ttttcttgtc
     5521 atcccaatta tcccaaccag ttgggtgaac atgcccacct atcttacacg cttcaaataa
     5581 gacatttgca tacggacgcc acggtcttcc taaatagacg tttctagtat caggttctgc
     5641 agtcaaggta cagctttcaa aacgataccc tgttttttga tttttcggcg tagatgctgc
     5701 tgtgatgtat ccaggatttt tactggctaa gctatggata tgacactgat gaaaaacggc
     5761 atctgcccca ccaaatatga aatccaccgt tccttcaatc cagcaatatt cataggtctg
     5821 acgacaatac tcaggattgg cagaagccgg tgtacaaaaa ggagtaccat ctttttgcaa
     5881 atcaggcaac ggacccgtgc ataaggtatc ctgataacca gatagtcgac agttttttaa
     5941 agatacgcga tctcctgaat tgaataaagc aacagcctgt cctacctttt ctcctggacc
     6001 tgctgtattt tcgatgtgaa gattttctaa tacgacattc gatccttcta agaaaaaagt
     6061 agcacttcgg aaagtcccta actggctacc gtcaagatac tcctgttttg caaaacgatt
     6121 accggagata actactgttc ctgctcccac aagtttaaaa ttggataatc tgctttctat
     6181 atattcatca tatcttcctt cttttactat caagaccttt ggatggctat ccggcaggcc
     6241 tgccaaataa tcgattcctt cttggatagt ttcaaaatcc gtcggggtat ctatagcatt
     6301 ctttccaatt atcagtttgt ctttcatcct cataaatgct cccttatgaa ttgtttccaa
     6361 atatttctca tttcttgtgt ttccatatga ccgcctgtta aagaaaaaga agaaaagttt
     6421 tctggactac ccattgattg atactttttg tccagccctt ttctcagctt cagtacgcct
     6481 tcgtccaagc acatcgtatc gttttttcca actaagctaa gacgagggcg cggagcgatt
     6541 ttttcttgga ttgctaacgt tgtataagct gtcaatagat ttggcacata gtaataaaac
     6601 ccatgatgat ctaggcggcg atgtagcaaa agtatttcga tgtccacctg tgccgctaga
     6661 tcaatacaaa ctttgacacg agaatccaat gctgtcaccc accagcttaa taagcctccc
     6721 atagaaagac caatcgttgc gagccgttga gtatctgtgt cttcccttgt ctccaaataa
     6781 tcgatcaagc tgatcacatc ataaatacgc attccccata acgtttttcc tgttaataaa
     6841 aattgtttga acaactcgct ttcagaaata cctccgcgtt cttcaaatcc ccaagcatca
     6901 atcgaccaaa cagcatatcc cattcctgtc agtacttctg caaaagatgg agaagctaag
     6961 taaggagcac catggatcaa ttcacttttc ccgactgaaa aatctccgcc gtgagaatgc
     7021 aggtaaagga ccggcggaaa cggtccttct ttttctggat aagcatagag ggctggtact
     7081 gtttccaatt catttaagta caattgatag tattcaagca taaaaccgtt ttgtttttct
     7141 cggtataatc gttttccact aggggtatgt ctgctagatg gagcacctaa aaaatcattc
     7201 aactgcataa gcaaagtttt tacgaacaac cagttcatcc atattttctg attgatttga
     7261 ttggaatttc tcaatactga tttctttagt attttctaat agaaatgctg gtccctcgtg
     7321 attttcaatc gacatctggt taaaggaaac cttacccgca aagccaatat aaaatccttg
     7381 tctgctcatt tcctctattc ctgtcatcat agcaggaaca ccgggaactg catcttgggc
     7441 catcgagaca tcgatattgt caaatgtgat ttcttgtatg aattgttccg ctaatccata
     7501 aataaaacct gcagaagcat ggacattcct tgccgtgata ttcgaaaaat ggatccttct
     7561 gaatgctggt gtccgttcat ctatcggata agcctttttt tcccatacat acggttcttt
     7621 ccctcgcgga ccacagaaat aataaagatt cagtataaat ggacacatca cattatccat
     7681 gactatattg gatactcgaa tatcttcaac gatcccgcct cgacctcgtc tagatttcaa
     7741 tcgtattcct cgatcagttt cttggaagat acaatttgaa atggtaatat tacgaatact
     7801 accactcatt tcacttccta atacgacacc gccatgtcca tggaccatcg tgcaatttgt
     7861 gatcgtgata ttttcgcaag cgatcctttc gtacgtatct tcagtccctg ccttgattgc
     7921 aatacagtca tctcccacgt caatatggca attactaata cgcacatttt tgcatgattc
     7981 tggatcaatt ccgtctgtat ttggtgaatc cgctgggttc aagatcgtca agttatcaaa
     8041 cgttgcatta ctgcaaagaa tcgggttgat cgtccaactt ggtgactgga tcaatttaat
     8101 atctttcaca gtaattcgat gacagttatg gaaactcatc aatttaggac gaggataagc
     8161 gagattatct ggttcatttc taaaagtatg ccaccatttt tttccatttc catctaatgt
     8221 accgaaacca gtgacagaga tgttctctac attttgagca tagatacagg aagcataaac
     8281 ttttcgatgg actccttccc aacgtgaatg aacaacagga taatcttttg gatcgtcaga
     8341 gaatttcaga accgctcctg cgcttaaatg aagttcgaca ttgcttttta aaaataaagc
     8401 tcctgttaga aattcacctg ctggaacaac tacggtcccg cctccgtcac ttgctgcagc
     8461 atcaatggct tgttgtattg cttcagtatt caattcatca attgatgcgc caaatgtcaa
     8521 aatatcatac atatttataa tgcctccact tcaatcaatg cttgcaagaa tgcaccaacg
     8581 cctttttgat cattacaaat aattggttca ctgatgtagt aagcatatga accatctcgc
     8641 tgatctgctc cgcctaaccc tgctacttga cagtttttat tcaagttcag ccagccttct
     8701 tttgtttcca gaacaaattc atcaagtaat ccttgatgtg atttcttcac ttgtggaagc
     8761 cattcttctt tgttcagcag tcccaaacgt attcctttag ccattgcgta aacaaacata
     8821 ctactgccgg aagcttccag ataatttcct ttccgttttg tttctgttgt tacttgatac
     8881 catgtcccgc tgttttcttc ttgatatgga agtaatgcag ccagtgtctt ttctagaatt
     8941 tctttcagca aagaacaatc tgtttcttca tgaagtaatt cataggtatc tgctgcagcc
     9001 atcaaatacc agccaatcga tcggctccaa aaatgatggg atagacctgt ttttggatca
     9061 gcccatggtt ggaccttctt ttcatcccat gcatgaatca gcaaatttgt ttttggatcg
     9121 aatagatgtt tataactgat ttcaaactgt ctcaaaacat cttggtaatc tttttcttca
     9181 ctaaaattct ggataaattc agcataaaaa ggagaaccca tatataagcc atccaaccaa
     9241 atttgataag gataaatttc tttatgccaa aaagctcctt cggatgtgcg tggatggttt
     9301 gccaactgct gggaaaggag ttctgctgct tttttatatt tttcttcgcc tgtttctttg
     9361 tacaataaaa agaaaagctt tcctgtattt acatggtcga tattgtattc ttcaacgctg
     9421 tagccacgga tcgttccatc ttcttgcacg aaatagtcga tattgtcttt tatgaaatca
     9481 aaataaattt tttcttgggt tcgttcccaa agtaattgga accctttaag tacgacgcca
     9541 tagtcataag accattttcc tttataccac ttttcttcat ataagcgagg tgttctttcc
     9601 atgatacttt ttgctaattt ctttgaccag ttttccatta tttgctctcc tttaaaaatt
     9661 aaaataggat ttgggacaaa taccaagaag tatttgtccc aatcgtctat attactgtgc
     9721 cgctgctttg tatgcttcct cgtactcttg tgtgattcga tcgcctcctt gtgctttcca
     9781 gtcttctaca gcttttttga atccatcttc gtcaagctgt cccatgatgt attgcaccgt
     9841 agcatcaatg acgatttttt caatttctga tcctacttca ttgttcgtcg gtgaatccaa
     9901 tggcacagaa ggatccatta cagcaaattc ttcattttct ttgatcaatt gattagagaa
     9961 ttctttttca gggtctgtat ctttgaaaga gaacgtcact tcactaggac gagagcttga
    10021 taacggttgc acctcttgtt gccaaagatc catatcgata aattcgatag aaccatcatc
    10081 attcaattca taatgcgttc cttcgatacc gccagtcatc aatttataca catcttcttc
    10141 taaaagatca tcaacaaatt gaagtaattt cttcaattgt gcttcgtctt ttacttgaga
    10201 tttgggaaat gctagcaagc cgccaatacc atttccttct gaccaaacat gataaccgcc
    10261 gccttcacca tctgagatct tgtttacagg cactaattcc atatcatctt gaataccttg
    10321 acccatcgtc ttcagattct tgatatccac cattccagtg tagattccag ttcttccttg
    10381 tgcaaattgt tcttgctgat cagtcttttg ggtaactgca aagtcttgtg ccaaatagcc
    10441 attctcaaat agatctcttg aaaaattcaa agctttcata tactcttctg tatcaaattc
    10501 tggagtgaat ttgccattat cgtctacagc ccagccattt ggtgctccaa aataagaagt
    10561 gaaaagttta aagctggtat aacgaatatc actaggcgca cgatcgccaa aaccaactgt
    10621 gtcgtccaca ccatttccat caggatcgtt ttctgtaaat tgtttagcta cttcataaag
    10681 ctcatccatg gtagttggaa catctagtcc tagattatcc aaccagtctt ttcgaatgac
    10741 taagccagca cgagcgtagt ctttttgtat cggtacgccg tacaaggcac cgtcgatact
    10801 cgcagcttcg attctatctt cagaaatttg acttaaattt ttatagtctt ttaaatattc
    10861 tgatacatcc cagaatacgc ctgattttaa cgcattgcgg acagaagagt ttttcaacat
    10921 cgcaatttga agtgtaacga tatctcctag ttcatcagaa gccaatgcag ttgttaatct
    10981 ttcttctttt gaagcatcag gaatccagtt gaatgtcagt tccgtatcgg ttttttcttc
    11041 gatcaagttc acaatcgtgt ctgtaggcgg tgaagcatga tgcaaaatat ttaaccaagt
    11101 gattttagct tttccatctt ctgtctctga agaactgtta cttgaactgc tgccgcatgc
    11161 ggcaagtgct agaattcctg caaaactaag acctaatttt acgagcgttg cttttttcat
    11221 aaattttctc cttcactgtt ttaataatat ttttgttttc ttctgttttt tctgtatatg
    11281 agttcttcca ttccaaatgg caaaatatag aatttgctgt ccacgtgact gcggaaaaga
    11341 agagactgcc accgataaag gttaaaacac ctggcattgt ttggatgatc atcagataaa
    11401 tccctaccat acttaatatc aacactaatg agtaatgaag atgtgagaat ccgaataaaa
    11461 atgccatttt cattttgaaa aaatatcctt tccaatcata atgagccatg attgggaaaa
    11521 tatagagtgc tgagagcaca tatatgaacg cagcaatata caaagcgatc ctaagatacc
    11581 aattgtgtat gaaagaaaga tccacaagta aaacaatccc gatggcgcca tatatccagc
    11641 tcatcagcaa ggtttcacga tagttttctt taaaatatgt gaagtacgtt ttcgtcaaag
    11701 gaaacgtttc ttttgagcgc agccattttc taatcactgc aaccatcgca tacgatgcag
    11761 gaccgaaagt aaaaactccc aaacctgcga tgactaaacc gccccacaga aaattcaaat
    11821 aaaccaactg taaaatacga tttagccttc cgtttatttt atctaatgac tgcagcatat
    11881 ctttccctct ctttctatct tagtaaacgc cgtcttctcc tagctttttg gcaatcttat
    11941 tagctaaaat taccataaac aatcctacga cccctttaaa caatcccatt gctgtactga
    12001 aacttaactg tccattcttc aatccagctg tataaatata ggtgtcgaaa atttctgcaa
    12061 cgcttcgatt caatgaattc aataacaagt acatatgctc gaaccctaag tctaatgtct
    12121 gaccgatttt tagaatcagc aaggtaatga ttactggacg aattgccggc aatgtaacat
    12181 gccatgtttt gcgtaagcgt cctgctccat ccatttctgc agcttcatac aactgtgtat
    12241 cgactgcagt tatcgctgaa aggtagatga tcgtagacca gcccaactct ttccagatca
    12301 cttgtccgat atatactgtg cgtgtccatt caggggatgt tagaaaactg attttttcaa
    12361 atcccattgt tgcaaggaat tcattcaata cccctccgtc aacattcaaa aatacataag
    12421 taatcgaaac aatgattacc caagacatga agtgagggat gtaaatgatc gtttgaatcc
    12481 cgtttttcaa tcgtttgttc gttacttcat ttaatagtaa agacaaaatg attggcatcg
    12541 gaaaaaacac gaagatattt aatccgaaca agatcaacgt atttcttaat aatgtaaaga
    12601 atgtcggttc tgtaaacaaa cgaatgaaat gtttgaaacc gacccaagga cttcccatta
    12661 ttcctaaaaa tggttgataa tcttgaaaag caataacgag tccccccata ggaagatacc
    12721 taaaaatcaa gaaataaagg actcccggca gaatcatcac atataaaaat ttattcgttt
    12781 tgattcctgc aattattttt tttcgtcttc tgagcttttc ttcttttttc gatagcgtca
    12841 gttgaaatgg ttcttgtttc tttacacttt ccataagccc ctccttagtt ttgtgaatgt
    12901 ttgctgctta tgacttcatg atagataact atctgaatta cgtatataat catttttacc
    12961 gtaagtaaaa acgcttacat agcaatgatt tttgtgctat acaaaacgat aaagtgtatt
    13021 ttttcacaaa taaactacgc atagcaaacc ttctattttt ttctatctga cacttagcat
    13081 aggaaatgat tatatacgta atctttcacc gatgataaat tgaacatata gaaacagaaa
    13141 catttaattt attttgagga ggagcctatc tatatgaaaa agaaagtctc tataggagaa
    13201 agaagtttta cacttttcaa caatacgttt ctaattttgc tagctttgat ctgtatcgtt
    13261 ccttttttaa atatcatcgc tacatctttt gcttctacac aagaagtcgt cgcgaaaaag
    13321 tttatccttt ttcctactac cttttcttta gacgcgtatc gctacattct ttctacgccg
    13381 accattttta gagcacttgc tgtttcgatt ggtgttacag gtgtcggaac gatcgtcagt
    13441 atgtgtgcaa cttcactgat ggcctacggt ctttccagaa agtatttgtt tggtcgaggt
    13501 ttcgtcaact ttcttgttgt attttctatg ttgttcagcg gggggatgat tccaacgttc
    13561 ttagttgttc gttctctagg gctggtcaat tcttactggg ccatgatcct gcctgtggct
    13621 gttaatgcaa tgaatatgat cattatgaga aactttttcc aagccttgcc ggatagtttg
    13681 gaagaatcag caaaaatgga tggttgcaca gattttggtg tatttttcaa aattatgctg
    13741 ccattagcgt taccgtctat tgctactatc tcgctttttt atgctgttac ttactggaac
    13801 acttatatga ctgctatttt gtatatcaac gattcatcta aatggccgat acagattctt
    13861 ttacgccaaa tcgtcattgt atcaagtggg atgcaagcag aaagtagtgc cgttgatgtg
    13921 attccaccag cacaaacagt taaaatggct gttatcgtta ttgcaacagt gcctatgctg
    13981 attgtttatc cttttgttca gaaatatttt gtcaaaggag cactcgtagg ttctgtcaaa
    14041 gggtgattat gtactgaacg caaaatggta gttgtgtcag aagtggtcag cttcaagaaa
    14101 taagaaggca tttacgaaaa ttgcttctta aatttttgtg aaatttcggc ttatttttcg
    14161 aagaatctac tcctggaaca ccgtgtatca gaggttgtga aagagacgtt cagccttgag
    14221 cgatgataaa aactctcaca aacattgcga cgagtaaccc acaggagcaa taagaagcga
    14281 ttttgaaaaa tagcttccca tatttcacaa aattatgcct tattgtcgat agcctgtctc
    14341 ttgaacgcca tttattcgta aaaaagaact gtgacaagac ttttgtcaca gttctttttg
    14401 cgtgcttagc cttgaaattt aactatccat aaatacaaat actagggtta ttctaacttc
    14461 ttttattgct tgaatcagtc cacaagaaaa acagcctctt gattcacaaa tatttttttc
    14521 gatattctcc cggcgtaatc gattccttct ttttaaagaa acgaataaaa ttttgcggat
    14581 ttcgatattg caatctttct gcaatctctt ttacggagag gttagtctct ttcaaccatt
    14641 tttttgctac ctctaaacga tgattttgaa caaaatcacc aaatttttct cctgtttcct
    14701 ttttaaatac attacttaaa tagttagggt tataatgcaa cctatcagcg atcacatcta
    14761 aagacagatc acgatcatat tctgtgtaga cgatatgcat aatattctcc gacaaatttt
    14821 taaattcatg atcagtgatt gcttgagagc attctgtgat tggcagaact aattgttcta
    14881 aaacaagttt ttctagttct ttgggatgat aattgttcag tgcttttaca taaatttgtt
    14941 tcatgttatc aaagtttttt gaatctactc ccaataactg tcctaattga acgagttcat
    15001 tgatcaagcg gaccgaaact acttcccgac tcaaaggatt tttattttgt gcaaacaatg
    15061 catcaatcaa ttcatgtact aaattcttca cttcctgatt tccagaacga atggcttcaa
    15121 acaatcgatt ttgttcttct atcggatatc taataagtgt tttttcatga gcagcaggaa
    15181 cgatttcctg ataaaaaata attgaacttg gaccagtatt tacacgataa tacagggctt
    15241 cttttgcccg atcaaccgat tgcttgcttt cattcagcgt ttcgaaccgg ttactgatgc
    15301 ctatactgat agttaaattc aagtattctt ttacacgttt ttgaatgagt cggcaatact
    15361 ccatgacttt tttgcttgct tctgcatcat ctttatccat tcgataaata gtggcttgca
    15421 tttcttcatt caaaacgatc ggcagcattc gattttcctt tggcacaatt tccgtgatca
    15481 tgttgttgat cgctaaaagt agcaaatcac gctccccacc gtgactatct tctaacagat
    15541 cgatttgaac caaagctgta taatagcagt cattttgaga actataacca aattgctgca
    15601 accgctgatc tagttctcgc ctttctactc gatttctaaa taaactaagt acaaatagcg
    15661 tttccagctg tggcttttgg gtaactaagt gtgcacttaa cgcttctttt tctcctatga
    15721 tccgactgac tgattgagcg accaaatcta gttctttccc actgaatcct tttgatttaa
    15781 gattcagacg ttcctgtatt tgagaaatcg gacgggcaaa tctgtccgaa aataagtagg
    15841 aaagaagacc aatgagtcct accagtgtta aagacactgc aactagcccg attctcagat
    15901 tgcggatcat tgcgccaatt gcactagagt caacttctaa tctgtaaatc caattattat
    15961 aatctgattt cagtactata tacgttttat ctttttctct tatcactttc agctgattgt
    16021 aatctagatc cacgtccgtt aatacagttg gttgctcttt attttttact gtctgagtag
    16081 taaaaaggtt gccaacttcg ttttcaatag aaagtatgtc ttctccactt tcaccaacaa
    16141 cttgttctat tgtatgtttc tctatcgtag caattcccaa tgcatatttt tctttttcaa
    16201 acattggcaa agaaatcacc atttcgattc ctttgttttt cttttgccaa aataaattat
    16261 ttttgttatt tatgtacttc tgttgatact tttcaatttc ctcttcggtc agttgactca
    16321 acgatccatt gattactccc catttaccag ccaaactgac taaatcataa ttattatcat
    16381 ccataacaat cgtttctata aaatttagtt ctttcctgat ttcagaataa gtggagtaat
    16441 ctgtatatgt cagcggatgc ttgaatatgt ccgagaaatt tttggtcgaa ccatacgtag
    16501 aaaacgaata ttcaatcgtt ctaattttcc attcaacgtt agttttaact tgcctgagca
    16561 aattcttttt gtctgttaaa taactcgatt cgatcgattc ataggtattc tggtaaatga
    16621 ataaactaaa tcctatgaca agtacggctc ctaataaaaa catcggtaaa aatattttat
    16681 aaaatactgt acttttcttc attttcttcc tctcctcctt ttaaaataaa aggctttcat
    16741 ttttctgtaa aattgatact catctaatta aatgagcatt ccccgctttc aagaatagca
    16801 taattaaaaa taaaaatcca agtaattttt aacgtgtgca atcttttcat atcaaagatc
    16861 tattgactta tcgtagttat cgtaatatga tgaatgcgtt aacaaaaagt gaaaaagtaa
    16921 ggaaaatgat ggaatggtaa cgatcaaaga tgtcgctaaa gcagctggcg tttctcatac
    16981 gacagtttct cgtgctttga ataacagcag tttaataaaa aatgaaacaa aacaaaaaat
    17041 acgacaacta gctaaggaaa tgaattattc gccaaactat agtgcaaaag gtttagtgaa
    17101 tcaaaaaaaa tatctaatcg gactattttt ctcaagtttg cataaaggaa cttcatcaaa
    17161 tttcctagca gattccattg caggcatcca ttcagtatta gacacaaatt acagtttatc
    17221 tgttgaaagt atcgatgttg ttgatttaac agaaatcaac ctgcaacgct acgatggact
    17281 gatcgttatg agtcaaagtg atgatgatca gcctttcatc gactatctca aagaaaaaga
    17341 atttcctttc gttgttgtca atcgtcacat caatgatcca gcggttatca acgtcactgc
    17401 cgatgatgcg gccggtgtta tgcaggcagt agattacggt attggatgcg gacatacgaa
    17461 gatcgcttat attggcggaa agaaaaattt tcgttcgaca aaagaacggg aaaaaggggt
    17521 attgaaaagt ctaaaaaaag cgagcatccc tgtcaatcca gcctattttt taacaggaga
    17581 ttacagctta gaaagcggcc tccaaaacat ggaataccta ttatcacttt ctgacctgcc
    17641 tactctggtt ttttgcggca acgacgacat agcgattggt gcattgagag ctgtacattt
    17701 agctggcctc cgtgttcccc agaacatttc tctgatcggg ttcgatgatt ctccagtcgt
    17761 ctcctatctc aatccgccgc ttaccaccgt acgtaagcct ttgaaagaaa tgtgtcaaga
    17821 aggaacaaag cttttactag atttgatcga aggcaggacg atcgaacaat ctaaaattga
    17881 gctctccacc ttgttgatcc ttcgtgagtc gatcaattac ttgtaaacta caagtaattt
    17941 tatataaaaa tgttaacgtg tgcataacac agaaaggaag tttatatgaa aaaactgaca
    18001 aaagaattag tgaaaaaaaa cgaagcacct gtaaaagttc ttcagtttgg tgaaggcaat
    18061 tttatgcgtg gatttattga ttggcagatt cagcagctga ataaccaaca attgtttaaa
    18121 ggtaatgttg caatcattca acctttagaa caaggtcttg gaaatatgat gaaccagcaa
    18181 gataatttat atacggtcat tttacaagga ctcagcaatg gcgaaatcgt tgatacttcc
    18241 gagattatca ccgtagtaga acaaatgatc aatccttatg atgaatggga tgcttatcta
    18301 tctctagcag aaaatgacga cttggcctat atcgtctcca atacaacaga ggcaggaatc
    18361 caattcgtgg agactgataa tttaacagat actcccccac agagcctccc gggcaaactg
    18421 acagcctttt tataccgacg tttcaaatta aacaaagctg gctttaccat cattccttgc
    18481 gaactgattg acagaaacgg cgaacgtcta aaagaaatca ttttacgcta cgcagaaaaa
    18541 tgggatttag aacctgcttt tgttgagtgg ttagaaaaag aaaatgtttt ctgttgtagt
    18601 ttagttgatc gcatcgtccc aggttatcct cgcgaagatg caggtgtttt caatcaacgg
    18661 cataactatg aagatcaatt gatggtcaaa gcagaaccat tcatgctttg ggtgatcgaa
    18721 gggcctaaag aattggagaa tcaactacca ttcaaacagg ctggcttgaa tgtcatcatc
    18781 acagatgata tgactcctta tcgtgaacgt aaggtgcatt tgcttaatgg accgcataca
    18841 gcgatggtcc ctcttgcttt gttggcggaa ttggaaactg tagaagaagt aatgaaagat
    18901 cctttattct ccgcatatgt tgatcagtta ttcaatctag aattgattcc tatgctttct
    18961 ttacctaaag atgaattagc catttatgct gaccaaatca aagaacgttt cttgaatcct
    19021 tttgcccatc acaaattaga agcgatttca ttaaatagtg tttcaaagtt cagcacccgt
    19081 ctgcttccag tgttcaagaa gtatatcgaa gaacaaaatc aggtaccacc gttaatcact
    19141 gtttcactag ctgcgctact tcttatgtat cgaggcgatc aagtaaagcc acatgatgat
    19201 gaaaagacga taagcgaatt tactgatgct tggtctgata atggaacagc tattcctcga
    19261 ttattgcaaa acgctgcgct ttggggagaa gatttgtctc aaataccgaa tgtgacagat
    19321 acagttcaag aaatagctgc aaagatcgat caaaaaggtg tccggtcagt attacaagaa
    19381 atgaaaggaa cgatggtcca tgcttgaaac cctgcaacca acaatgaaat taaatcgacg
    19441 agacagtgtg gtcgtcgcat taacccctat tccaaaacaa acacaactga cgattgatca
    19501 gcaaacgatc accactcttg aagatatccc gcaaggtcat aagattgcat tgggtgattt
    19561 gaaagctgga gacaacgtga tcaaatatgg ctatccaatc ggtcatgtga cgacagacgt
    19621 aactgccggt caatggctgc acactcataa tgtaaaaacc aatctttctg gtgaattgga
    19681 ttatcgatat gaaaaagatg tccatccaag tcattacctt tttgagaacc gaacatttca
    19741 aggttacttg cgaaaaaatg gaaaagtagg gattcgtaat gatttgtttt tagtacctac
    19801 cgttgggtgt gtgaatggca ttgctgaact gatcgtcaaa cagttcaagg aaaaacatcc
    19861 agatctggga cagtttgacc atattacgat tttaaagcat ccttatggct gttcgcagct
    19921 tggaaaagat catcaaaata ctcgagaaat tttagcggac gcagtaaatc atcccaatgc
    19981 cggaggagtt ttagtatttg gattaggttg tgagaacaat acagttcctg aattcaaaaa
    20041 aattttaggt gaatatgatg aagaacgagt gaaatttcta gtggctcaag atgtttacga
    20101 tgagatcgaa caaggtgtgg ctctattaga agagctttta acagcggcgg aaaatgacca
    20161 gcgcatatca gtcccgctct ctaagttgaa tgtcggtttg aaatgcggcg gttctgatgg
    20221 tctatcggga ataactgcta atcctttact tggtgcattt tctgactact tgattgctca
    20281 aggaggcagc acgatcttaa cagaagtgcc agaaatgttt ggcgcagaac aagtattgat
    20341 ggcacgagcg gaaaatgaag aagtttttga ctcaatcgtt gacttgatca atgacttcaa
    20401 acagtatttc ttgtcttacg gggaaccagt ttacgaaaac ccatctcctg gtaataaagc
    20461 aggtgggatc actacattag aagataagtc tttaggctgc acgcaaaaat caggaagctc
    20521 tcctgtcgtt gatgtgctac aatatggcga aaaaatcaga aaaccagggc ttagtttatt
    20581 gcaagctccc gggaatgatc ttgtagctgc ctctgctctt gcttcatccg attgccagtt
    20641 ggtcttgttt accacaggaa gagggactcc gtttggctca tatgtcccaa cggtcaaggt
    20701 atctacgaat acaactcttt ttgaacgcaa aggtcattgg atggatttta atgcaggcgt
    20761 cttattagaa caaccaatgg aagtaatctt agaatcgttt gttcaaaaaa tcatcgctgt
    20821 agctagtgga gaagaaacga aaaatgaaca aaatgacgtt cgagaaattg ccatttttaa
    20881 aaacggagta acactttgat tttcataaaa cagtataaaa agaaagaagg aagatttatg
    20941 tttttatctg atgatttttt attaaaagac gattgggcga aaaagcttta ccattcctac
    21001 gcaaaaaaca tgcccattat tgattatcac tgtcacttag atccaaaaga aatttacgaa
    21061 aacaacaatt tcagcaatct gacagaagct tggttaggag gcgatcatta caaatggcgc
    21121 ttgatgcgag cttgcggcgt gcctgaagaa aaaatcacag gaaatgcttc tgattttgaa
    21181 aaatttttag cttggtgtca aaccgtacca aaaattatcg gaaatccgct ttacagttgg
    21241 tcacatcttg aattgaagcg tttcttcggt attgacttgt tgataaatga agaaaatgca
    21301 gagaaaattt ggacacaagc aaatgaaatt ttggcagctc ctgattttcg ccgtcgggaa
    21361 atcgcaaaaa aatcgaatgt agaagtgatt tgtacaacag atgatcccgc agacgacttg
    21421 cactatcatc agttgcttgc tcatgaagaa ccagatttga aggttctacc ttcattccgt
    21481 ccagataaag cactcaatat tgaaaaagaa ggatttaatg catgggtcac actactggaa
    21541 gaagcagctg accaaacgat caccacctat caagaactga tagaagtctt agggcagcgg
    21601 atagaatact tccatcaaca tggatgtcgt gtttccgacc acgctttaga catcttacgc
    21661 tataaagaag cggatgaaac agtattagaa gagatttttc aaaaagcacg agctaacaaa
    21721 ctattatctt cagatgaaat cgatgcttat cgtacagaaa cactgataca tttgacccac
    21781 ttctatcatt ctcataattg gacgatgcag ctgcacatcc atgcttatcg taactgtaat
    21841 acacaaatgt tggaaaaatt agggcccgat accggctatg atggaatgaa tgatctttct
    21901 ttgactattc ctctgcaaaa attattgaac cgtgcagaag aaacggatca gttgccaaag
    21961 acgatccttt attcattgaa tccgaatgac tatcctgcga ttttagcttt gatgggctgt
    22021 ttccaaaaag aacagaatgg aaaactgcag ttaggttctg gatggtggta caacgatact
    22081 cgagctggta tgcgcgatca attgacacaa tttgctgatg gtagtgcttt ggggaatttt
    22141 gtcggcatgc tgacagattc aagaagtttc ctgtcctaca ctagacatga atatttccgt
    22201 cgtgttcttt gtgaattttt aggcgaaatg gtcatgcgag gagaagcacc tgaagatgaa
    22261 caacttttag gaagtctagt tcaagcgatt agttatacaa atgctaaaaa ttactttggc
    22321 ttttttgcct aattgattaa tagtaacagt aacgaatcaa aaacaggata gtgaaataac
    22381 ggaatccgtt tatccacgtt ttagagaaga agacggacca aatttgctcc gtcttcttct
    22441 cttgctgttc tgcttcattg cctataaacc tttatgaaag aagagataac atgcctacta
    22501 tccatattgc cggtgattct actgcggcag aaaaagatga aaacaagcgt cccgagacag
    22561 gttggggaga aaaaatctct gcttattttt cacccgagat ccagatcaat aaccaagcaa
    22621 agaatggacg cagcaccaaa tcatttatcc aggaaggaag gttagcccgt ctagcttcac
    22681 agtttcagcc gaatgatttc ttatttgtac aatttggaca taacgaccag aaaatccaag
    22741 aagaaagagg tacgcttccc ttcggtagct atttaaaaaa tctgcagata ttttttgaat
    22801 gcgcaaaaaa agcgaacgtc caaatggtct tactgacatc tgtgacgcga cgagattact
    22861 tagaaaacgg aacattgaat ccagatatac ttggagacta tccaaaagcg atgcgtgcat
    22921 ttgctgagaa acaccatatt cctcttttag atgtgttctc aagatcacaa gaattgttcc
    22981 agaccttctc aaaagaagag acaaaaaaat tctacctgca tcttatacct gatacttgta
    23041 aaaattaccc agaaggacta aaagacaata cccactttag tccagaaggc gctgataaag
    23101 ttgcccgaat aattgttgaa ttgataaaag aatcccgtct gcctcttgct aattatttac
    23161 aaaaaggagt ttgagatgtt tccattaaat ttaggcatcc gtgcccacga tttagatgtt
    23221 cgaaatcgtg aagaaatcgt tcaaaaagta tcacagcttc acttgtccca tatccaattt
    23281 gcaccacaaa aagcatttcc tgaacaactt aaattaactg acatgacact tgggagtgct
    23341 gcctattttg gtgaatactt ccaaaaaaac aatatcgaac tttccatttt aggatgctat
    23401 atcaacctat ccagcaaaaa tcaagaagtt cgtgcacaag cattggcaac tttcaaacag
    23461 caaattgctc tgtgctctgc ctatcaagcc aagatgattg ctacggaaac cggtagtgtc
    23521 tctgtcggtt acacaaaaga aaattttact gaagaagctt atcagatcgc cagaaattct
    23581 atcattgaat tagtcgctca tgcagaaaat tttggcgtaa ctgttgctgt tgaagcaggg
    23641 atcaatcatc caatctataa ctatcagtta gctaaacgtt taatcgatga agtagcttca
    23701 ccaaatttaa aaatcatttt agattgtgcg aatttgatgc ataaagaaaa tcatgctgat
    23761 caggaaaaag tgatccgagg tgccttggac aatttacatg gttatattac agcgcttcat
    23821 ctcaaagact atatcatgga aaatggaaag atccgcatgg tacctgttgg caagggctgt
    23881 ttagatttcc gcccggtact gcattatatc aaatatgagc gtcctctaat gtacgccact
    23941 cttgaagcta ctcctgagat ttatgttcct gaagcaatag aacacttgca gtttatttac
    24001 caagattgtt aaggagaaga ttatggagga acgaatacag aaaagttatc aaaaatatca
    24061 tgcaaatgct tttcagacat tgctgcatct ttatcaggga aattattggc gttttgccat
    24121 gtcaggactc ttttttatta tcaaacatct tccatcttgg ttaatgccga tcgctattgc
    24181 caatgtaatc aatgcagctt catcgaaaga ttcttctcag ttgcaggcca tttatttcaa
    24241 cgcttttttg atggcttgtc tgattggtca aaatatattg acgaattatt ttcatgtaaa
    24301 ataccatagc acttcgatcc gtcaagtaga agctggacta agagcgaccc ttatcaagaa
    24361 aatccaagaa ctatctattc cttatcaaaa agaattgcaa tctggacgaa tccagtcaaa
    24421 gatcatccgt gatgtagaag ctatccaaac gttgtcctct caagtatttg tcagcggatt
    24481 aaatatcgct gtcaatttat tcgtagcttt aggtatcacg ctttataaaa gtcctgttgt
    24541 tttcatgttt ttcttattga ctgttcctct cgccgcattc gttactctct ttttccgtaa
    24601 aaggatcaga cagtccaact cagacttcag gcaagaaatg gaagagactt ctgcaaaagt
    24661 gatcgaagtg atcgaattag caccgattgc tcgagctcat gggttagaaa aacacgaaac
    24721 aactcgctta aataagcatt ttactcaaat ctatgaaaaa ggattccact tagatatcct
    24781 gcaatctgtt ttcggatcag tcagctgggc tagttttcag atatttcagc tgatttgttt
    24841 gattttcact gggatattag ctttacaagg gcgcatccaa cccggcgatg tcgtgatgta
    24901 tcagacgtat ttcacgacga tcgtcaattc cgtttctgga atgatcacgc tgattcctac
    24961 gatttcaaaa ggcatggaat ctatcacttc tattggcgaa ctgttgattg ctggcgatgt
    25021 agaaacttat cacggtaaga aaaaaatcca acatttggat ggaaattttc gttttgagga
    25081 tgtttctttt ctttatccgg actcactcaa accgattatc gatcattttt ctctagaggt
    25141 gaaagctgga gaaacaattg cttttgttgg gccatctggc tctggaaaat ctacactcct
    25201 taacttattg attggattta tccagccgac ttcaggaaaa atttttctag atgatcagcc
    25261 tttggatgaa ctggatatgc gaagtgtgcg aaattattta gccgttgttc ctcaaaccac
    25321 tttgttgttc tccgcttcga tcaaagagaa catcacttat ggcttgaaaa acgtatcaaa
    25381 agaacgttta gatgaagtca tcgaagctgc gcaactttct tcactgattg accagcttcc
    25441 tgatggtctg gataccttag taggcgaaca tggcaacaag ctgtctggtg gccaaaagca
    25501 acgtatctcc attgcacgag cgttgatacg tcagccaaaa atcattttgc tagatgaagc
    25561 aacctcagct ttagataacc agtcagagaa aaaaatccag caagccctga attacctgac
    25621 agaaacaccc actactttta ttgtggctca tcggctttca acgatcaaag aggcagataa
    25681 aatcgttgtg atcgatgaag gaaaaatcgt tgaaataggt acttacgaaa aactgttagc
    25741 tcaaaaaggc tatttctacc gcttgaaaaa tgaatgacaa aaaataggag ctatgacaag
    25801 acttttgtcg tagctcctat ttctgaataa acggtgttct cctgccaact cctcggtcac
    25861 aagtcacaat agtcatcaaa catgagaagc tgttttttga aattgttcct tgtaactcag
    25921 agttaaacgg taggctcacg acctcttggt aaatggtgtt caagagacag cctatcggca
    25981 ataagaagca attttgtcat cgctcaaggc cgaacgtctc tttcacaccc tctgttgttt
    26041 cataaaatag ttttattcgc tgtaaaggtc gcgaactggt gtcatgatga tacggttcaa
    26101 gtcattgatg attgtactga agccttgctc tttttgcatc aagtcattga ttacttcttc
    26161 tgattggatt ttcacagcca tttcttgtgc tttcattgca tcttcgtcgc tgaactcttg
    26221 tccactcatt tgtttttctt gtaattcgat ttgcatcgct tggaagtctt tgaacagttt
    26281 gtgtgcttct tcgtttgctt gtactttctc gtacgcttct ttcaacgctt tgaattctgg
    26341 taattcacgg atctcacgtt cgatttgatt cgcgctatcg tagatgtttg acatgttatg
    26401 ccctcttttc ttttagtagt tactctctat tgtaacatgg ggttattcgt tttcaaaggg
    26461 gctactctcc ttgatatttc cgccagaggt ctttagccgc ttcaccaatt ccctgaacgc
    26521 cttctttcac catatcgccg aagtttttca gcccttcttt tgcttgttca ccatattgat
    26581 tcaagttatc ttgccaattt ccagactctc cattagaatt tcctgtgttt ccaacttgat
    26641 ctgcagctac cacttgtcct cctgtggcat aggcatctgc tacactaaat cctgtttttg
    26701 aagtatacgg aaggatgcct tctgccgctg atttgaagac tttcccgaca acatttccac
    26761 ttgtcccttc caaatagtgc agcttgctgg attcttcgta acccatccat gtcgaaatca
    26821 ctacgtctgg cgtgtaaccg atcatccatt ggtcatttgc ttttgtggca tcgaaattag
    26881 tttctgtggt tcctgttttc cctgctatcg tataacctgc aggattcgca gcagccgctg
    26941 ttccatttga aaatgtacca agaagcatgc tggtcatttc atcggctgtt tcctttgata
    27001 tgacctgttt tggttttttg tttgtgttat cgacgatcac cgctccagtc gaatcaacga
    27061 ttttcgtgat aagatgcggc gtatacatct gaccatcatt ggcaaatgca ctgtaagcac
    27121 ccgccatgat caatggagat actccttttt ccagtccacc aagggctaag ccataatact
    27181 tatcagattt tgctaaaggc aggccgaatt cttctgcttt atcatatcct ttttgtaaac
    27241 cgatctcatg caaaagccat acagtaggca aattcaaact ttgtgcaaca gcttgataca
    27301 tcggtacttc tccttggtat gtcccgtcaa aattatgcgc atcgtaataa ctttgcggtt
    27361 cgtctttcaa gatactggaa ggcttgtatc cagcttccag tgctggtgta tatacgctta
    27421 atggtttgat cgttgatcct ggtgaacgac gcatctgtgt agcaaagctg aatcctctaa
    27481 agacatgatc tcctcttcgt cctactagtg cttgtacacc acctgtattc ggatctaaag
    27541 ctactgaagc agattggacc atggcaccat ctgaagcatt tgctgggaat aatgcactgt
    27601 tttgataagt agcttctaat tgcttttgat aatcctgatc taaagctgta tagattttat
    27661 agcctttatt catgacatct tcttcatcaa gaccatactc gttaactgct tcatcgatca
    27721 ctgcatcaaa ataataagga taacgataac cagaatctga attatcgtat gtgtcattaa
    27781 gatacgcgct gatatctgtc tggctttctg cttgttcttg ttcttttgtg atttttccgt
    27841 tttcttccat tacacttagt actgtgtttt tccgatcatt cgcatttttt gggtgatcga
    27901 tcggattata aataccagga cctttcagca ttcctgccaa agtagcagcc tcagacagcg
    27961 taacattcgt tgcatctact ccaaaatact ttctggaagc gtcttggaca ccccataccc
    28021 cgttgccaaa ataggcattg ttaagataca tggtcaagat ttcttctttg ctgtacttct
    28081 tttcgatctc gattgccaaa aataattcct tcgcttttcg atcaaacgtt tggtctaatg
    28141 tcagatacgc atttttggca agctgctgag tgatcgtact tcctccgccc cctgatgttc
    28201 cgccagacaa gaccattcgg actgctgcac gggcaatccc tttgatatca aacccgtgat
    28261 gctcgtaaaa attcctatct tctgtcgaga ttactgcatc ttggatatag ggagaaatcg
    28321 cgtttaattc tacatatgtt cctttttggg cgtacagtgt gccagcttct tcgtttttct
    28381 tatcataaat gactgtcgat tggcttaatc ctgattttaa cgtttcgaca tttgcttgtt
    28441 ttgctaaaat aaagaggtag atacttgtca ctaagacaca gatcaaacca atcagcaaaa
    28501 tcaatttgtt cacatgatat tttttccaga tgcgctttcg ccatcgatgg aattgtccga
    28561 gatatggtct gatccaacgc caaaaactga tcaaagcagc ttttatcttt tctaaaatgc
    28621 tttgaaaatc catttgcttc ttctcctgtc tatttgattc aatcgtctta ttttaccata
    28681 gtgaaagagt gttttcttcc tgcttaagtt gtagttcaat atgttttttt acgttagtgc
    28741 aagtagagta acataagaat gaacagaaaa atgtaagttt tttcttaaat atgttttaaa
    28801 aatggtacaa ttagtctgcg attgcactga aaggagtaca cttgtgaaaa tcacgatcac
    28861 tttacctatg aaccatcctg aaacaacaat tcgcgacttg cttgaaaagg aatggttagt
    28921 tcctagaaag gttcgacact ttttgcgtat acgtaaaaac gtttggatca atcaacaacc
    28981 tgctttgttc catcatgaag taaagggcaa tgaccagatc acattgttat tcgaagaaac
    29041 agattataat tatcaagaag ttcaattagg agatgcgaag cacatccgtg ttttatatga
    29101 agatgagcat ttgattatag tggataagcc tgcaggaatg aagactcatc caaatgaacc
    29161 gacagaaaac agtaccttac tcaatcattt agctgcttat ctcgacacta aacagcaacg
    29221 accttacgtt gtccaccgct tggacaaaga aacaagcggt gccgttttat ttgcaaaaaa
    29281 tccatttgtc ttgccgatac ttggaagaat gttggaacaa aaaatgatct atcggagata
    29341 tcaagccatc gtatggggag aaattgccaa agatatgacc atcaccaata aaattggacg
    29401 agatcgccat gatcggagaa aaagggtcgt agatgataaa aatggtcaga gcgccttgac
    29461 acatgtcact gtcaacaaag tactttctga acaaacacaa gtctattgtg ttttggaaac
    29521 cggacgtaca caccagatca gagttcattt agctcataag ggacatccga tcgttggaga
    29581 ccctctctac caaaatagaa cagctgaacg actgatgctc catgcattag aactgcacat
    29641 gatccatcca tttactcaag aaaaaatagt cggtaaagct ttacctggat tatgggaata
    29701 gaaataaaaa agaacagagt cttatcagca gataatgata agactctgtt ctttttgtgt
    29761 aatttgtctg cttacttttg atgttcgtgt tctgctaaca gcaaactacc gataatccca
    29821 ctttcattcg gcagtcccca acgaacgata tattcactta cttgaggtgt ttccatataa
    29881 ccacccatca attcaacaaa ttgctgacgg attttttgaa ggagatgatc ctgattcatt
    29941 accccaccgc ctaaaataat cttttcaggt gctaacgtca acgtatagtt gatcagtgct
    30001 tgagcaatat aataagcctg aatagcccaa acaggatgat cttgcggcag atcttgccct
    30061 ttgatccctg tgcgtgcctc aatagacggt cctgctgcta atccttctaa acaatcttta
    30121 tgataaggac aagtaccttc ataatggtca tctggatggc gtttcaccca aatatgtccc
    30181 atttcaggat gtgcgatccc tgaaaaaatt tcttcattga tcacgacacc tgcaccgatt
    30241 cctgttccca cggtaaggta aatacaacta ttttttcctt gtgctgctcc ttgcttcaat
    30301 tccccatatg cagcagcatt gacatccgtt gtccaagcag caggaatatt gtagcgttcc
    30361 ttgattgtgc caagaaaatc gaattgtgcc cacccttttt tgggagttgc caaaacgtat
    30421 ccgtaattat cttttgtttt gtcgatacca atcgggccga atgagccaat cccgattccc
    30481 ttcaattcat aacgatcaaa aaattcaaat acctgatcca atgtttcttc tggtgttgtg
    30541 gtagggatac ttaatttttc taaaatttct ccaccatctg aaaccgcaca cacaaatttt
    30601 gttccgcctg cttctattcc gccatacatt cgttttcctc ctcgaatccg attgtccgaa
    30661 tctcataagg ttggattatt gggacaaccg cttgcttcgt tggttcttca agcagattca
    30721 atatgacagt ttttccattg tctttagtta tttccaattt ttctaaatga ctagacatat
    30781 tgaaacctct tactactact ttatcactaa acttgctgcg tttcaaagct gtaattgcaa
    30841 acgtttcact ttttatcgtt aaatattgtt gttttgaaat caatgttcct tcatgatgtt
    30901 tgatttgttg agtggaaaaa ggaatctgtg ctgcgtatgc atgcaaataa gtagagaatt
    30961 tttcttctgg tccatgtagt tcaatgctgt aattgaaccg atgctcacct agacactgtg
    31021 cttctggtgt tggaaaatat ccccaatcgc ctagttctcc cacactgcga agtagcgtca
    31081 ctgcgatttg tccgtcttct gttacttcat attcattcaa accaaagttt ccgactgtta
    31141 caccgtactc ttcatcatga atattgacaa atgcttgctg atgttgcgga ttcgtggggt
    31201 tttcccaact tttagacaca tggtttgggc gcttcaccac ttcaaaaata ctatctgctt
    31261 cgtgatgttc cacatgtaac tttgtaggaa acaagacacg taagcgatga tctttcatct
    31321 gattgtcgag agtcgtttca aagtcgattt ttttactatc ttttctgatt gtcatgattg
    31381 tttttatttg aaggattcgt ttttctttgg atcgttctgc ttttcgataa cgaaattcca
    31441 caaccatctg ctgttctttt tctaaacgct cgtctgcaga aacagggatt tccatttcct
    31501 gtagaatact tatttttgct atttccggtg tatctactaa tacttttact tcagagtttt
    31561 ctttgttact ggataggatt ggctgatcac agaaaggttg tttaaagata tactcatttc
    31621 ctatatcgcc gatatcttca aaaatatgca gattattcaa ctttcgtttg tttttgcgat
    31681 cttccattgt aatggtacca tttttttcaa tcgtcaaatg tagagggccg ttttcgatga
    31741 ttcgtccact ctgatgaatc attgtttcct tttcttctgc tttcgcttcc ccttctacaa
    31801 gcgcgaatgt ttcccaagaa aatgctggca tgttttctaa tggtaaagtc actttgatca
    31861 ttcttgccat atatggcaca cggaaacggt ctttcggcaa atcataacca aattggacag
    31921 tttcttcaga taaaatagct ggtatagtgg ccccactttt tgtttctaca tgatacttac
    31981 gtttaggcaa attttctaac tcttgatata gttgttcagg tattccttca gcgaatcttt
    32041 ttctttctaa ttcgatggtt atttctgctt caccagtttt atccattcct gcagtattta
    32101 caatgacaaa tggaaagctg tcttttggga atccggtcgt atcgattgct gcggtcagct
    32161 gttctaagga atcttgtgca agatactttc caacttcatc tgctttttca aaacgaggta
    32221 tcatttcccg atggacagaa tcgactgaac aaccgcaaat actgtcgtga ggatgatttt
    32281 gcattaatgt cttccaagca taatctaatt gatcatgggg atagtttcca ctaacttcat
    32341 aagccatcgt cgcaagtggt tctgtaatat tttctagctg tcgctggacc ttggtattcc
    32401 attgttttaa gtaaacccga gcagatgctg tattggctaa agtataccag ccatccgttt
    32461 cttgacttgt caattctcct tctactgagc ctaagtcttc tggcacatct ttttgaactg
    32521 cttccagata gtcagtaaaa ttagaatgaa taaactcgta atctggaaac agttcatttg
    32581 cgagatggat tgcttttgat atatcttttt gtaccggttg atgatcaaca ccattcatca
    32641 tcaataagtg gttcgtagag gcatattgtt ctgcatccgc taatttttgc ttccaaaagg
    32701 caagagctgc ttctttttct actggaatct cattcccgtt gctataccaa ttggcaaata
    32761 gcagaccaaa gattgctgtt tgatcaggtc ctttccacca catctctgag tattgggaag
    32821 aatagttttc tgcttccaaa acttgattat caaagccaat tggttttaca ccacggccaa
    32881 acgctgctgc tgaaatacca gcttgtttca ttaattgagg cgtctgtccc atatttccaa
    32941 atgtatcggg aaaatagcct aacatgacag gggttcccca tttacggctt tcttccatgc
    33001 caatcagcat atttctcacg ttcgattccg agctgataag aaaatcatct tgcaagatat
    33061 aaaatggtcc aattctcaat tttccttcat tgattgcttg ttggattgcc tgtctttttt
    33121 ctggccttac ttgaagatag tcatctaaaa taatcgtttg cccatccaaa tgaaaactgt
    33181 tgaaggacgg atcaacttca aatagctcta gtagatcgtc tatcaattca atcagtcgca
    33241 tgtgatgctg ttcgtaagcc atgtaccatt cacgatccca gtggctatga gaaatgatat
    33301 atactttctt tttcatttac gatacctcct acttttctac tctaatatca aagtaatcca
    33361 tgactaattc gcaaaacatc atatttgccc aagaaaacca ttctctcgta taattttcag
    33421 gattgttcac atcgaatcct tcgtgcataa ggtgtgttcc tgcgtcagtt gctacaagta
    33481 aattcaatat ccgctctttt tcagatttgt ctgacgttgt cattccttcc atagctaatg
    33541 caatcggcca aacgtatttt tctggcgtat gggaggatcc aattcctttt gcgtactttc
    33601 cttcatagaa atatggattt tctttactca acaatgttct tcttgtttgc agataatctt
    33661 cctcttgttc agaacaatat cccaagtaag gtgcggcgat cagattgggc acattactgt
    33721 catccatgac tgtggcgttt cccattccat ctacttcata tgcataaatc gtttgccctt
    33781 tttgattaac cgttcgtccg tgattctgaa tcccctcttg gatctctttc ttcaatttct
    33841 gtgtttgctg tacaatttct ttatcatcga gtatcgtttg aaaaatttct tccacataac
    33901 ctaacacgac caccgcaaac atattggatg gtaccagata tccataacgg caagtatcat
    33961 cgctaggacg gaaaccagac catgtcattc ctgtaaaagc tgtttttgca cctcgcccgt
    34021 cattgatcag cgtatcttct tttcttgtcg tgtctcgtac aaatgtataa ggagagtgtt
    34081 catgatcttg ctctgttttg aacacgtcca agattttttt gataccttca ataaaagatt
    34141 gattgaattg atcagttttt cctgtatttt tgtaaagaag ataggccaac tgtattgggt
    34201 agcataaaga atcaatttca tattttcttt cccagatcca atcgttcatt tttgtatgat
    34261 ctgtttggtg gccagcaccg ttagcttctt cgttgaaggc attcgcatat ggatcgatat
    34321 tgatataaaa aaattgcttt ttgactaacc cgctgatcat gtcagctaat tcctcatctt
    34381 cttttgcaat cactaaataa ggacgaactt gtgcagtaga gtctcttaac cacatcgctg
    34441 gaatgtctcc cgtcaataaa aaggtggttc cgtcattttg ctttttgact gttgtcaaca
    34501 gagtattggc aaatgctgct gtaaaattct ttgcccagtc cgcatggata tccccacatt
    34561 tttgtgtgat atcttccata aatttctgca ccgattttgg tatatcttta taagccacta
    34621 ataaaacctt ctttctattt gatatatctt taaagattat acttcataca gccaattatg
    34681 tctacgcttt caaaaaatat ctttttattt actttttcat ccattatggt attatttgaa
    34741 tatgatatat cattaagagg tgtttatatg aatcaaccat tgtaccagac aatatttaat
    34801 gatttgcaaa cggctatttt aactggtgaa ttacctgtag acagccaact accaactgaa
    34861 aaagaacttt cacgtaccta taaagtaagc agaatcactt ccaaacgtgc attgactgaa
    34921 ttagagcaat taggacttat ttatcgcgtt cgcgggaaag gcagtttcgt taaatccgct
    34981 tctccggaac agattcttgc ttctcctaca aaaacacgac ggattctgtt tcttcttcca
    35041 tatgtcgctg atttgtctgt tggtgatttt agcaagggat tgaaccctgt gatgcaagat
    35101 aaacagattg atgtcatgat gacgacgctt gactttctac aaaataaaac agcaaaagag
    35161 ctaatcaatg aatttgacgg attgatttat tatgctcttg atacagaaca acatcttgac
    35221 ttactttttg agctttcatt gaaagaattc cctgtcgtca tattagataa aaaaatctat
    35281 gagttacctt ttcctaccgt tctttccgat aatttccaag gcggtgcgct tgctgcctct
    35341 tatttgatcg aacagggaca ttcaaaaatc gcttatttgt ttggtacgca gactcatcca
    35401 caatccgttc gacagcgtta ccttggttac cttgaagcat tggacaaagc tggtttgact
    35461 ttccacactt cactggatga caaaagcgcg acattagatg aattgctttc ttatatcact
    35521 aagtatgaag taactgcttt tgtttgtgaa aacgatgttg tcgctatcca gaccatgaat
    35581 ctcttgagac agcatggtta tcatatccct gaagattttt caattatcgg atttgatgat
    35641 atccaagcag cagctcttgt tgacccgcca ctgacaacca tcgcacaaga ttttgaacag
    35701 ttaggtaagc ttgcgggaga tgctttggtc acttggttag agacaaaaga tcgaccatcc
    35761 gatagtaaat ttccagtttc attcatcaaa agacaatcaa caaaggagaa ataaaatgaa
    35821 tatcggtcaa atagatacta gacatgcaac agcgaatcag ccaaatttct ctaatgggaa
    35881 ctgccagcct tatactggcg ttccttttgg gatgaattat tttgcaccgc aaactacgga
    35941 tcaaaacggc agctggtggt ttcatcctga tgaccgcgtt tttcaaggct accgtcttac
    36001 ccatcaacca agtccttgga tgggtgattt cagtcatatg ctgtttacgc cgatcaatgg
    36061 aaaactacag gagaacaccc ttttccatgc tcaaagttct taccgcccag aagaaagtat
    36121 attttgcccg actcatctgt cgatctgtca attacgttat ggcatctgtt ctacattgat
    36181 acctagcatg tatggcggaa tcttatctat tgattacagc aaaaatgaca gcggtttact
    36241 tttatccttt ccgggacgcc atcagctctt tgttatagat ccttacacag tggaaggaaa
    36301 aatcacacat tttgctggtt cagaagatcc tgatttttct ttttatttta tattgagatt
    36361 tgaatatcct ttaacaattg atgagttagc tgtttccaac aaaaatagcg actcgatccc
    36421 gttgtatttt gaacagacaa aaaaacaaac tatccgattt gggacatctt ttatcagctc
    36481 ggaacaagca gcttataacc tggaactaga acaaaaccaa agcaaagaga cttaccttgc
    36541 tcacagtcaa gaacaatggc agtcctattt tgatcgtata cagctcacac accatgatca
    36601 acaacaattg cggacgtttt atcataattt atatcgggtg tttttatttc cacaaacatt
    36661 ttatgaaatt gatcctagtg ggcaaaaaat ccactatgat acgactagtc gatctataaa
    36721 acctggtgtt ctctatacaa ataatgggtt ttgggatacg tataaaacgg tttatcctct
    36781 ttattctttg attgctgtag aaaaatacga agaaatgctg gaaggttttt tgaacagcta
    36841 tagagaaagt ggctatttgc caaaatggct gtctcctgat gaacgtggac ttatgcctgg
    36901 gacattgatc gatgcggtca ttgcagatgc tgctgtaaaa gatatccgac gagacttgat
    36961 gcctgaattt ctaacggcaa tggaaaaagg tgctacggtt caaagcaaac atccaaatta
    37021 tggacgccaa ggaacagaag attacttgac atatggctat gttcctaatc actaccacga
    37081 atcagtcaat cacacattag actatgctta tagcgatttt tgtattagcc aagttgccaa
    37141 ggtactagaa gaagaggaaa tgacgaagaa atatcagcag caatcattga actatcaaaa
    37201 tatctttgac tatgagtcag gatttatgcg ttcaaaagat aaagatggca agttcagaga
    37261 accattttca gctaatcgtt ggggacaaga ttatgcagaa ggaagtgctt ggcaaagcag
    37321 ctttgctgtt tatcacgatt tttcaggact gatcggaaaa tatggtggca gtgagagata
    37381 taaagaaaaa ctcattgagc tgtgcaatca gcccccaacg tttgacccag gaggatatgg
    37441 ttttgagatc cacgaaatga gtgagatggc cgcgatagaa tttggacaat tagctatttc
    37501 aaaccagcca agttttcact atccttactt gttcagttat ataggacaac ctgaaatggc
    37561 acagccactg cttaaacagt tgatgactca gaccttcgac gattctccaa tgggttaccc
    37621 tggagatgag gacaatggaa gtatggccgc ttggtatatc ttcaatagtc tgggatttta
    37681 tcctgtcacg cctggaaccg gtgaatatgt tatcggtatg ccattaattg acaaggctgt
    37741 tttgcttttg tcaaatggaa aggaattgac gattgaagca acgcctaatc ggccgcagca
    37801 acaatttatc cataaaatca aacggatgga caaagaatat acgaaattat attttaccca
    37861 tcaagatttg ctgaaaggcg gcacgatctc ttaccaatta gggatcatcc ctcaccctaa
    37921 gaaatatcaa gcaaaagatt tgcctttttc cttatcaaac tataattaac aagaggttgt
    37981 gaaaaagctg tcctgcatca agtaataaga acagccaagc aaaaatagat cctcatattt
    38041 ttcgtttggt tggtcttatt accgcagatg caagcttttg aacaccgttt attgagaggt
    38101 tgtgaaatca gcgttctgac ctgagtatta gagagacaaa tggcaaaata gttcctcata
    38161 ttttgccatt ttttgagcta ataccgaggg tcagctgatt gaacaccgtt tagt
//
LOCUS       DAKBVG010000006        32315 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000006 DAKBVG010000000
VERSION     DAKBVG010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 32315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32315
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.006"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            67..1965
                     /locus_tag="OUD09_000255"
     CDS             67..1965
                     /locus_tag="OUD09_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HCT9691567.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(2264..2983)
                     /locus_tag="OUD09_000256"
     CDS             complement(2264..2983)
                     /locus_tag="OUD09_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HCT9691568.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            3320..3652
                     /locus_tag="OUD09_000257"
     CDS             3320..3652
                     /locus_tag="OUD09_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9691569.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            3639..5642
                     /locus_tag="OUD09_000258"
     CDS             3639..5642
                     /locus_tag="OUD09_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HCT9691570.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            5661..6131
                     /locus_tag="OUD09_000259"
     CDS             5661..6131
                     /locus_tag="OUD09_000259"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HCT9691571.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            6152..6736
                     /locus_tag="OUD09_000260"
     CDS             6152..6736
                     /locus_tag="OUD09_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HCT9691572.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            6751..7737
                     /locus_tag="OUD09_000261"
     CDS             6751..7737
                     /locus_tag="OUD09_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HCT9691573.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            7727..8038
                     /locus_tag="OUD09_000262"
     CDS             7727..8038
                     /locus_tag="OUD09_000262"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HCT9691574.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            8060..9841
                     /locus_tag="OUD09_000263"
     CDS             8060..9841
                     /locus_tag="OUD09_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HCT9691575.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            9834..11210
                     /locus_tag="OUD09_000264"
     CDS             9834..11210
                     /locus_tag="OUD09_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HCT9691576.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            11217..11852
                     /locus_tag="OUD09_000265"
     CDS             11217..11852
                     /locus_tag="OUD09_000265"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HCT9691577.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            11882..13237
                     /locus_tag="OUD09_000266"
     CDS             11882..13237
                     /locus_tag="OUD09_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HCT9691578.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKITIHSKIALTVTGTLLLGGFVVFFFTERNGETLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(13429..14823)
                     /locus_tag="OUD09_000267"
     CDS             complement(13429..14823)
                     /locus_tag="OUD09_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCT9691579.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQKLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQNKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            14915..15886
                     /locus_tag="OUD09_000268"
     CDS             14915..15886
                     /locus_tag="OUD09_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HCT9691580.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKDKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSATLTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            15883..16664
                     /locus_tag="OUD09_000269"
                     /pseudo
     CDS             15883..16664
                     /locus_tag="OUD09_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
     gene            complement(16833..17204)
                     /locus_tag="OUD09_000270"
     CDS             complement(16833..17204)
                     /locus_tag="OUD09_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCT9691581.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TTPSYYFVIDPDGYKIEVIRG"
     gene            17338..17781
                     /locus_tag="OUD09_000271"
     CDS             17338..17781
                     /locus_tag="OUD09_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HCT9691582.1"
                     /translation="MDKPIFGEKKQSANYQTRYAAYIVIERDKRIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(17824..18633)
                     /gene="yidA"
                     /locus_tag="OUD09_000272"
     CDS             complement(17824..18633)
                     /gene="yidA"
                     /locus_tag="OUD09_000272"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HCT9691583.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(18652..20019)
                     /locus_tag="OUD09_000273"
     CDS             complement(18652..20019)
                     /locus_tag="OUD09_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCT9691584.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHKVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHETITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(20003..20833)
                     /locus_tag="OUD09_000274"
     CDS             complement(20003..20833)
                     /locus_tag="OUD09_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HCT9691585.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            20996..21433
                     /locus_tag="OUD09_000275"
     CDS             20996..21433
                     /locus_tag="OUD09_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HCT9691586.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            21509..22129
                     /locus_tag="OUD09_000276"
     CDS             21509..22129
                     /locus_tag="OUD09_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HCT9691587.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     EKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            22318..22824
                     /locus_tag="OUD09_000277"
     CDS             22318..22824
                     /locus_tag="OUD09_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HCT9691588.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            22841..23740
                     /locus_tag="OUD09_000278"
     CDS             22841..23740
                     /locus_tag="OUD09_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HCT9691589.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKARR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            23827..24712
                     /locus_tag="OUD09_000279"
                     /pseudo
     CDS             23827..24712
                     /locus_tag="OUD09_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
     gene            complement(24904..24975)
                     /locus_tag="OUD09_000280"
     tRNA            complement(24904..24975)
                     /locus_tag="OUD09_000280"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24940..24942),aa:Arg,seq:ccg)
     gene            25035..26369
                     /gene="rpoN"
                     /locus_tag="OUD09_000281"
     CDS             25035..26369
                     /gene="rpoN"
                     /locus_tag="OUD09_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCT9691590.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            26518..27609
                     /locus_tag="OUD09_000282"
     CDS             26518..27609
                     /locus_tag="OUD09_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HCT9691591.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            27650..28651
                     /gene="gap"
                     /locus_tag="OUD09_000283"
     CDS             27650..28651
                     /gene="gap"
                     /locus_tag="OUD09_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCT9691592.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            28761..29951
                     /locus_tag="OUD09_000284"
     CDS             28761..29951
                     /locus_tag="OUD09_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCT9691593.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            30055..30810
                     /locus_tag="OUD09_000285"
     CDS             30055..30810
                     /locus_tag="OUD09_000285"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCT9691594.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            30926..32224
                     /gene="eno"
                     /locus_tag="OUD09_000286"
     CDS             30926..32224
                     /gene="eno"
                     /locus_tag="OUD09_000286"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCT9691595.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT        10670 a         5727 c         6870 g         9048 t
ORIGIN      
        1 tattcattga aaatagagca taacaacttt ataatgttga gtataccaat ttttggaggc
       61 gttttaatgg accgctattt agatttactt tcagagaagt tccctacaac ggaagatgtc
      121 atcacggaaa tcatcaatct cgaagcaatt ttgcagcttc caaaaggaac agagttattt
      181 ttgagcgata tacatggcga atttcctgct tttgatcata ttctgcgtat cggttcagga
      241 aatcttaaag aaaagatcaa agaccttttc tctgaacaaa tgacggaaac ggagatcagc
      301 caattcacta tttttacggc ttatccagaa tatgtgttga cgactgattg gtacaaaaaa
      361 caagataaat cccagctgat ccatcgtttg atcgatcttt tacggtttac gacagtcaaa
      421 tacactcgat caaaagttcg aaaaggattg ccgaaagaat atagctatat cattgaggaa
      481 ctcttgtata tcgatgatcg tatcaacggg aaaaaagact atgtaaaaaa aatcattgaa
      541 caacttgtct tgatgaagga agaagaaaaa tttttaacaa aacttgcgca gacgatccaa
      601 aaatctgtca tcgatcattt gcatatcgtg ggtgatattt ttgatcgagg aactcaagca
      661 gccaaagtaa tggatcgaat catggatttt tcctctgtag atatccagtg ggggaatcat
      721 gatattttgt ggattggtgc gtattgcggc tctgaggcat gtttattgac gcttttacgg
      781 attgccgcac gctacaacta tttatttgaa ttagagaaag aatatggatt aaatttacgt
      841 ccattatttt cttttgccca tcaaacctat cagaagaatc cagtatttac ccctaaaaac
      901 aggaaagatc tatctgaaag agagcaagaa gaattagaaa agatccatca agcactttct
      961 atcctccaat tcaaatcaga acatcagttg ttgaatcgac gacctgaatt taaaatggac
     1021 gatcgtaaat tgttggaaaa aatcaactat gaggaatcaa cgatcgtttt agaaggtcag
     1081 ctctatgagt tgaaagggac ttgttttcaa acgatccagc cagatgatcc taaaaaattc
     1141 ttggaagagg aacaaaaagt gatcgatagt ttgatgtatt cattccagca ctcgttaaga
     1201 atgcaaaaac acatgacgtt tttgatcgaa aaagggggaa tgtatctcac gaacaatcag
     1261 catgtactgt tccatggctg tatacctgta gatgaaaagg ggcagctctt agcatttgaa
     1321 ttagaccaat cttatcgcgg aaaagaactg ctgggatttt ttgaaaaaca gataactgaa
     1381 agcgcaaaag atttgactgc aaatgaagat ttggcgaccg atttgatttg gtatgcgtgg
     1441 agcgggccat tttcgccttt attcggcaag agtaaaatgg caacttttga gcgctatttt
     1501 ctcacagagc ctcatacgca cgtcgaaaaa agcaatccct attatcactt gcgagatgaa
     1561 gagtggttca gtgaaaaaat tttggcagaa ttcgatgcaa aagaagaagg ttcagcaatc
     1621 atcaatggac acacgccagt aaaagtaaaa aaaggagagt caccgatcaa agcaaacgga
     1681 aaagcatttg ttattgacgg aggtctatcc aaagcttatc aaaaaacaac cgggattgct
     1741 ggctactcat tactatgcaa ttcttacggt ttccaaatcg tgacacatta tccgttcttg
     1801 cctattgaac aacaatttgc aaaaaaatct gatcaaacga atgtaaaaaa agtgatcgag
     1861 cagcagctcc cacgcaaact taatcggaat acgacaaaag gaatggaatt acagcaacag
     1921 attactcaac tccaaaggct gttggattat cgaaaggacg attgagaaaa atgctagcca
     1981 acattgctca atagactaaa aagcgactgc aacaaaaggc gtgtcgcagt ctccttcgga
     2041 attagctcaa aaaacggtaa aatattagaa gctatttttc tgtttatttc tctaatactc
     2101 aggtcagggc gctgatttca cgacctcttc cttaaacggt gttctcctgc caactcctcg
     2161 gtcacaagtc acaatatcca acaaacatga gaagctgttt tttgaaattg ctccttgtga
     2221 ctcagagttg aacggcaggc tcacgaccta atcagaaaaa cgattaaaat tcattcacaa
     2281 agaaatcgaa taaacgctgt tcagatgaat ctacacgatg agaaagttcc ggatgccatt
     2341 ggacaccaag gattttgtgc atattatctt tactttggac tgcttcaatg agtccatcgt
     2401 tggaaaaggc gatggctttt aacaaaggag aaagatcttt gactgcctga tgatgatagg
     2461 aattcacaaa gtatgaatca gggagaaggt cacttaataa actgtccgaa acgattttga
     2521 cttcgtgagt tgcaaactgc ggggcagtgg gctgttgttc gtgttttacg gaccattttg
     2581 gatataatga aagatcttga taaagtgttc cctccaacac gacattcaat agttgaagtc
     2641 ctcggcagac tgcaaaaatc ggttttcttt gtttcaatgc ttccaagatc aatgcctgtt
     2701 cgaattggtc tcgttgaata tttgtttccg tcagttttgg atgagggtct tccatgtaaa
     2761 gttgcggact aacatcctga ccgcctgcaa gaatgagttt gtcgattttc tcaattagtt
     2821 gaggcgctga atctggagac atgactggca gaataagagg taatccttga gcatctaaaa
     2881 ctgcatccac aaatccctgc ggtgtatagc ttacttgatt tccttggaaa gtatctgtcg
     2941 ctcgaatcag ttgattcccg gcaatgccga tgattggttt catttttatt ccccctaaag
     3001 tagactaatt gtaacaagga tttaaaaaaa tttaaactat gtaagacttg cccctatact
     3061 tatctgttct tgctgtgtta ctatctgtaa agagaatttt cgtgtgaaat tcatgacgat
     3121 agttgaaaat attgcttaat ttgttataat aaaaagtaag cagaaataaa aaggttgggc
     3181 gtaagcttca aggatctagt tcttcaaggg gtgagaagga cattttattg aaggggcctg
     3241 cctatactaa gttatcttta gaggtacata ctttcaatga aaaaagaaag catgtacctc
     3301 ttttttaaat cgaaaggaag tgaagcaagt gttagaagct ttggaacaaa tccgaaaagc
     3361 agaagaagaa aatgagaagc gtgctcgaac cctacggcag cagttgaaga cctatgcaga
     3421 agaaaaagag caagaattgg agataatcca aacgaacagt cggcaacagc tgaaacagtt
     3481 ggtagaagaa aaagaacaat tcgaaaagca agaattagaa aaagaaaaaa tgaaattgaa
     3541 agcagaagca gacaaggtgc aagcaacttt gaaagaccaa tatcaaaagc atcagagtca
     3601 agcaatcggt gcaatcattg aaagggtgaa agaaacttat ggccgtcact aagatggaaa
     3661 aagtgaccct catttcagat aaaagaaatc aggagaggat tttacaagca gtacaaggaa
     3721 tccaaaatgt tgaaatccgg gatctgtatc aaaatagaac aaacaaccag tgggtagaaa
     3781 aatattttcc agataccacg attattgata aagaagcaaa attaagcgct ttaggcaatc
     3841 ggctgaaaga catccaagaa gctatccaat tcatacaaca ccacggcgat agccgtcaaa
     3901 agaaactcca tttgaaacgt caagaacttt ccttacaaga cttggaagca aattattccg
     3961 aagaaaattt catgaataaa ttagaagagg tccttgattt aaaagaaaag tgggaacagc
     4021 taactgaaaa acgtgatcgt ttagaagaag aggaagaatg gcttcttcat tggcaacagc
     4081 tggatattgc tccaagtatg tatgcaagta agacgaccat ttttgagatg gggactgtga
     4141 gtgcagcaaa ttttgaaagt ttcaaggaag aactaagtaa gcttgatgac gtttatatgg
     4201 aagaaataga ttatacgcca aaacaagttt atctttcgta tgttgcttta aaaaaatcag
     4261 ttgctgctat agaagaaatt gctgggaaat acgggttcaa taaagaaacg tatccttatg
     4321 aacaaaatcc taaagaaaaa ttgcaagaaa ccaaacagca attatccatt gttcacgagc
     4381 agcaaaaaaa gataacgaca gcgttaggtc agtgcagcgg ctatatccaa gaatttgaat
     4441 gggtagaaga agtcacactt gccgttgcag aacgggaaaa agtaaaagaa cggtttatcc
     4501 atgccactta tctgatcgtt ttgcaaggtt gggtagatgc agaagaaaaa cagaatttga
     4561 tgcatgtgtt aaacgaaact gtatcagaag aagatatata cgttagtttc gaaacaccaa
     4621 actcagaaga gattcagcag gaagtcccta ctaaattgaa aaaccatccg cttatcgaac
     4681 cttttgaatt gctgactgaa atgtacagtc tgccaaaata tgaagaagta gatccaacgc
     4741 cttggatgac accattttat cttgtcttct tcgggatgat ggtcgcagat gttggttacg
     4801 gattgctgat gctcattggg acaatactag ctcaaaaact atttgtttta ccccgcggga
     4861 tgaaacgttt cgtaaaattc tttgagattc tctcggtacc atcgatcatc tggggattga
     4921 tctacagttc atttttcgga caggcattac caaaagaact atttggtatc cgcttgcctt
     4981 tcccaatcct atctacgacg gatgacgtca atacgatttt gattttatcc gtgattttcg
     5041 gtctgataca gatcttggtt ggattattcg tggcagcaaa ggaaaatatc aggaggaaag
     5101 attacttgag tgctgtcagt gatggttttg cctggcaagg gatcctgatt gggatcgtta
     5161 ttgcattgat tggtgtcatg ttgataaaaa atacagcatt tgtttatctt ggcgcggggc
     5221 tatcgatcat tgctgcttta tgtatcttaa tcgtgccgat tatccaatcg ccgtctaaag
     5281 tcaagggtgc tgccaaagga gcgtataatc tttacggatt gactggttat atcggtgatt
     5341 tagtcagtta tacacgtttg atggcactag gtatttcagg cggaagtatt ggcgcggcat
     5401 tcaatatgct agttgctttc atgccgccag ccgctcgatt cagtgtaggg atccttttga
     5461 tcatcgcttt acacgcactg aatatgtttt tgacactgtt aagtgcctat gtccatggtg
     5521 cacgattgca gtatgtcgaa ttttttggaa aattttatac aggaggcgga cgtgcctttg
     5581 acccgctgaa aacggcggaa aaatacgtca acatcaatca taagaaaaaa aacaataaat
     5641 aattggagga tttttataca atgatggatt acttaattag tcaaaatggt ggaatggtat
     5701 ttgcagtatt agcgatggca acagcgacga ttttttcagg gattggttca gctaaaggtg
     5761 ttggaatgac aggtgaggca gcagcagcgt taacaacaag tcaacccgaa aaattcggac
     5821 aggctttgat tctgcaattg cttccaggta cgcaagggtt gtatggattc gttatcgcct
     5881 ttttgatttt tatcaactta ggaaatgata tgtcagttgt acaaggattg aactttctag
     5941 gcgcttctct gccgattgct tttacaggtt tgttttcagg tatcgcccaa gggaaagttg
     6001 cagcagcagg tatccaaatt ttagcgaaaa aacctgaaca tgctacaaaa ggaatcattt
     6061 ttgctgcgat ggttgaaaca tatgcgattt taggtttcgt tatttccttc ctattagtat
     6121 tgaatgccta agcataaagg aggaaaagca gatgaacgcc attgaaaaaa tcatctctca
     6181 gatgaatgaa gcagcagagc aagaaagagc ggcgttagag caagaagagc gaatgaagat
     6241 cgatcaaaac tttgaacaaa aacgtacgca aatcgaaaca gaacatcaaa aacaaaaaga
     6301 aaaacaaatc gaattacttg aaaaaaaata ccgtcagctt aggaatcgcc aacaagtaga
     6361 agtacgacaa gaaaatttga atgcgaagca ggaattttta cgacgattgt ttgcagatgc
     6421 aatagctgaa atggagaatt gggatgaatc tgaacaaatc cagtttatca aaaattcatt
     6481 gtattcatta cctttgacag gcaaagtaac attcattgct ggagaaaaat cagcagctta
     6541 tctttctcag acattgctgg atgaatggaa caatgaactt ccctttatga tggttttaag
     6601 tgatgaaact gttgctgatc aagcgggttt tctgataaac gatcaaggag ttcagtataa
     6661 ttttcttttc agcagccttg tacaagatat ccaaggaacg atgagttttg agattgccaa
     6721 tcaattattc gaataaggag ggatcagtgt atggaatacc acgaactaaa tcccttgatt
     6781 cgcggaagag agcttgaact tttgtccaat gagacatttg aacggatgat tcaaacaaat
     6841 tctgtcgaag cacttggtga aatattgaaa tccacgattt acagtccata tatttatgac
     6901 ggatatgaat atgacttcga acagaatctt ttaaaggagc agagccgtct atttcgctgg
     6961 ttaaaggaat cagcaccgga gcctgaaatc gtttggatct atacgatgcg ctatactttc
     7021 cataatctga aggttttgac aaaagccgag atgacaaatc aaaatctgga ccatttgtat
     7081 atcgacgatg gattttattc attagaaacc ttgaaggatg ccattcatac gcaagtttca
     7141 aatgaactcc ctgaaagcat tatggacagt atacgagaag tacgtgatta ttttgaagag
     7201 tctaccattc ttcagggaat tgacgtgatt tatgatcgac agtttttgac agagcagcga
     7261 cggcttgccg ataagctagg atatcaagaa ttgctgacag aagtcattgc ttttattgac
     7321 ttgaccaata tcacaacgat ggctcgagga atattgcaga aaagaacata tgggttcatg
     7381 acgacggtac tttccagctc gggaagtatc gaaaaagaag tatttttgaa ctttgtagat
     7441 aaagatatgg aagattacac gaattttcta ttagagacag attatgctga ttttctgcgc
     7501 cctgtcatcc gtgagggaaa tcttgatctc gtaaagcttg aacagttgaa agatgattat
     7561 ctaagttcat tgtatcaaga agcacaaacc caggctttcg gaccactgcc actactagcc
     7621 tttttgaacg caaaagaggt agaaagcaaa aaccttcgtt tactggttat cggcaaaaga
     7681 agccatttcg aaccagaaac aatcagaaag cgggtgagac aagtctatga cgcataagat
     7741 cggtgtagtt ggcgacaaag attctgtttt gccattcaaa ttgtttggct ttgatgttcg
     7801 ttatggtaca accaagcaaa ccgttcgtca ggcaattgaa gaaatggcaa agaatgagta
     7861 tggcgtgatt tatgtcacag agcaatgtgc agcattgagc ccagaaacga tcgaacgtta
     7921 caaggaacaa atgatacctg ctatcgtatt gattcccaat catcaaggat cattagggat
     7981 agggctggaa gaaattcaaa aaaacgtaga aaaagcagta ggtcaaaata tcttatagta
     8041 tgaaaaggag atagaaattt tgcaaattgg aaaaatcata aaagtctccg gacctcttgt
     8101 aatggctgag aacatgtctg atgccagtat ccaagacatg tgtctagtgg gagatctagg
     8161 agttatcgga gaaattatcg agatgcgtgg cgatgtggcg tctattcaag tatatgaaga
     8221 gacttctgga attggtccag gcgaaccggt tcgctctact ggtgaagctt tatctgttga
     8281 attaggacca gggatcattt cacaaatgtt tgacggaatc caacggccat tagatacctt
     8341 tatggaaatc acacaaagta atttcttagg gcgcggagtt caattgcccg cattggacca
     8401 tgagaaaaaa tggtggttcg aaccaacagt agaagccgga gaaacggtga gtgctggaga
     8461 tgttatcggg atcgtagaag aaacaaaagt tataaaacat aaaatcatgg tgccaaatgg
     8521 gataaaaggt acgatcaaaa caatcgaaaa aggttcattc actattgatg aaacagtatg
     8581 tgtcattgaa accgaaaatg gtgacaaaaa tttgacgatg cttcaaaaat ggccagtaag
     8641 acgtagtcgg ccaatcaaag aaaaattaaa cccggatgca cccatgatca ctggacagcg
     8701 agtgattgat acattcttcc cagtgacaaa aggaggagca gcagctgtac ctggtccttt
     8761 tggtgcaggg aaaacagtcg ttcagcatca aattgcaaaa tgggccgatg tggacatggt
     8821 tgtctatgtg ggatgtggcg agcgtggaaa tgagatgacg gatgttttaa atgaattccc
     8881 tgaattgatt gaccctaata caggagaatc gttgatggaa cgtacggtat tgattgctaa
     8941 tacatcaaac atgccagtag cagctcgaga agcttctatc tatacgggaa tcacaattgc
     9001 ggaatatttc cgtgacatgg gttatgacgt tgctatcatg gcagactcca cttcacgatg
     9061 ggcagaagca cttcgtgaga tgagcggacg tttggaagaa atgcctggtg atgaaggtta
     9121 tccagcttat cttggttcac ggcttgcgga atactatgaa cgatctggaa gagtcattgc
     9181 attaggatca gaaggtcgcg aaggaagtat aaccgcaatc agtgctgtat cgccgtctgg
     9241 aggggatatt tcggaaccag tcacacaaaa tacattgcgg gtcgtaaaag tattctgggg
     9301 tcttgattcc agtttagccc aaaaaagaca ttttccttcg atcgattgga tacaaagcta
     9361 ttctctttat tcaacagaag tagggaagta tatggatcat atccttcaag ctgactgggc
     9421 atccatggtc accgagggca tgcgtatttt gcaggaagaa cagcagctga atgaaatcgt
     9481 gcgtttggta gggattgatt ccctttcaga taatgaccgt ctgacattag aagtggcaaa
     9541 atccattcgt gaagattatt tgcagcaaaa tgcttttgat gatgtcgata cattcacttc
     9601 aagagagaaa caattcaaaa tgttgaagat gatcttgacc tttggtgaag aaggacgccg
     9661 tgcgttgtcg ttaggtgctt atttaagtga gatcatggac ggtaccgtat cgattcgtga
     9721 aaggatcgga cgaagcaaat atatcccgga aacagaatta gaaaaactag atcaaattat
     9781 tggtgaaatc aagcaaacga tgcaaacgat cgtttcagaa ggaggcatga gcaatgatta
     9841 aagaatatcg tacgatcggt gaagtcgtcg gtccattgat ggcggttgaa aaagttgctg
     9901 gtgtcaaata tgaagaatta atcgaagtcc gtatgcaaaa tggcgagatt cgtcgtggac
     9961 aagtattgga agtccaagaa gataaagcaa tggtccaaat attcgaagga acaagtggta
    10021 tcaatttacg tgactcttct gtacgatttt taggccatcc tttagaacta ggtgtatcgg
    10081 aagacatgat cggtcgagta ttcgatggtt tgggacgacc gaaagataac ggaccagaga
    10141 tcctgcctga aaagcatttg gatattaacg gagaagtaat caatccagta gctcgggatt
    10201 atccagatga gtttatccaa acaggtatct ctgcaatcga tcatttgaat acattagttc
    10261 gtgggcaaaa attaccagta ttttctggct ctggtcttcc tcataaagaa ttagctgctc
    10321 agatcgcgcg acaagcaacg gtactcgatt catcagatga tttcgccgta gtctttgctg
    10381 ctattggaat cacttttgaa gaagcagaat ttttcatgga agattttcgt caaacgggcg
    10441 caatcgaccg ttctgtcatg tttatgaatt tagcaaacga ccctgcaatc gagcgtatcg
    10501 ctactcctcg tatggctttg actgctgcag aatatcttgc ttatgaaaag ggaatgcatg
    10561 ttttggttat catgacagac atgaccaact atgcagaggc attgcgagag atttctgcag
    10621 cacggcgaga agttccagga agaagaggat atcctgggta tctttacacg aacttagcta
    10681 ctttattcga acgagcagga agaatccgcg gactaaaagg atcggttact caaattccga
    10741 ttctaacaat gccagaagac gataaaactc atccaatccc tgacttgact ggttatatta
    10801 cagaaggaca gatcatcttg actagagagc tttacaagag tgggattcag ccaccaattg
    10861 atgtactccc gtctctttcg cggttaaaag ataagggaac tggacccgga aagacaagag
    10921 aagaccatgc agctacgatg aatcagcttt ttgcagccta tgcacaagga aaacaggcaa
    10981 aagaattggc cgtagtttta ggtgaatctg ctttatctga tgtagacaag atctatgcga
    11041 agtttgctga gcggtttgaa aaagaatatg tcaatcaagg attctacacg aaccgttcga
    11101 ttactgaaac acttgatcta gggtgggaac tgctatcgat gttgccaaga accgaattaa
    11161 aacgaatcaa agacgacatg ctggacaaat atctgccaga agggaagtga gttctgatgg
    11221 cacgattgaa tgtgaatccg acccgcatgg aattgactcg attgaaaaaa cagttgacaa
    11281 ctgccacaag agggcataaa ttattaaaag ataagcaaga tgaactgatg cgtcaattca
    11341 tcctgttgat tcgaaagaac aaccagctgc gtcaagaaat ggaagagtct atgacagaag
    11401 caatgtctga ttttgtattg gcaaatgctt cgatcacgga gcctttcatc gaagaattat
    11461 ttgttttgcc ggcggaaaac gttgatttgt ctatcgtaga caaaaatatc atgagtgtaa
    11521 aagtacctgt gatgaatttt gaatatgatg aacagctgac agacacacct ttggaatacg
    11581 ggtatttgaa ttccagtgcc gaactggatc ggtcaatcga tcggtttact accctgcttc
    11641 caaaactttt ggaactgaca gaaatcgaaa aaacttgtca actgatggcg agtgaaatcg
    11701 aaaagacacg tcggcgagtc aatgcattag aatatatgac gatccctcaa ttagaagaaa
    11761 cgatctatta tattcgtatg aagctggaag aaaacgagcg cgcagaagta acaaggttga
    11821 tcaaagtaaa aaatatgggt accactgact aagtggtatc cttttttaaa agaggaaaac
    11881 gatgattaaa caaagcaaac aaataaagaa acgcttgccg gctgttcagc ttctcgcatt
    11941 aggatttttt ctcctgattt tagttggcgg cggcctactt accctaccat tttttagtaa
    12001 tagtgggcat gggacaaatt ttatcgatgc gcttttcact gcaacttctg ctgtctgtgt
    12061 gacgggattg acaacattga atacagcaga gcattggaac agtgctggtc aatttttgat
    12121 catgcttttg attgagatcg gcggtcttgg ttttatgatg gttccgattc tatgctttgc
    12181 attgataaag aagaaagtca gtctcagcac acgtattata ttaaaagaag cgctgaatct
    12241 agaagaagta tcaggtgtga tgaagctcat gctttacatc ctgaaattgg cctttgtgat
    12301 ccaactgatt ggtacggttg ctttatcttt tgtattcgtg cctgaatttg gctggttgaa
    12361 aggtatctgg tacagtttgt tccatgcaat ctctagtttt tgtaatgcag ggtttgattt
    12421 gttaggagac agtttagcca atcaccagca aaatgtttat ttgatcatgg tggtatctgc
    12481 tttgatcatt tctggtgggt taggcttcat cgtatggcgt gatttactgg aatatcatca
    12541 gaaaaagaag ataacgattc attcaaaaat cgcattaacc gttacaggta cccttttatt
    12601 aggcggattc gttgtgttct ttttcacaga gcgcaatggg gaaacgctag tggaagggaa
    12661 tatgtttcaa cgattggcaa acaccttttt tatgagtgtg acacctagaa cggctggata
    12721 ttattcagtt gattacctcc atatgagcca tgcaggtctg attttaacga tgtttttgat
    12781 gtacatcggt ggaacatctg ggtcgacggc cggcggattg aaaacaacta cgttaggtgt
    12841 gttgctgatc cagatgcgtt cgatgcttaa aggacgtacg agagcggaag catttggccg
    12901 taccatccga caaggcgcag tgttgcgggc acttacttta ttttttgtta cgttgacgtt
    12961 gtgtgtagtc gccatcatga ttttatcggt gactgaaacg atacctaaaa cttctggcat
    13021 cgaatatatt gcttttgagg tcttctctgc gtttggtaca gtaggtctta cgatgggatt
    13081 gacgccagac ttgactgcaa tagggaaatt gatcattata gcgcttatgt acattggacg
    13141 agttggtgta ttgactgtgg tcttctctct gatgacaaaa gcacaaagaa cacaatcaaa
    13201 atacaaatat ccagaagaaa gtgtcatgat tggataattt catcttctga cagaaaccta
    13261 gtgatttagt aaaataagaa aataaaataa tttttcttgc ttcttggata ctttaagtgc
    13321 aaaatgttcg tttatttttt gaggcactat ccgttgttga acctcgggta gtgcctcttt
    13381 cttattgaaa ggaatcaata agatttgatt cctttattta tcaataggtt ataattcata
    13441 tttttttatt ttttctttta cgatacttag tccagaagct aaaatttcaa ggttattaga
    13501 ggatagggaa attcgaacga atgcatctgg ttgcgcacgt gtagtaaagc gtctggaatg
    13561 ataaagtcgg ataccgttag caagaaagtc attctcaatt atcttaggat caatctcagg
    13621 atcaactgga agaattcgat agtaagggac acgattcgtt ggtttttcta ctttgaaaag
    13681 agaatcaaaa agctgattgg ctcttttcgt aagacttaat ttagtttcta agatcctatt
    13741 tgcttcattt gaatggagga tttgagtgat gacttccaca tctaatccag aagttttcac
    13801 atttatatta aaaattgctt gttctatctt gttttggaca tgtactggaa ctacaagata
    13861 agcaactcgc aatccagaac aaataaattt tgtcattcca gctaagtaga ttgtttggtt
    13921 aggtaataat ttttggaaag gaggaatgat cgtatgttga tagtaagttg ttaaaaatga
    13981 atggatatca tcttcaataa cccatagacc ttctttttca atcacttcgg ctaattgctc
    14041 tcgtcttttg tgagagattt gaaaccctat aggattatta caagaaggca taagaaagac
    14101 accatgaatc ttcttttttc tacattcctg aatcaatata tcaggtcgca ttccttgctc
    14161 gtcaaaatca attggaacga tctctaaatg atataactga gccaattcaa tgaaattagc
    14221 gtatgtatat cggtctgttg ctattctatc acctggagag aaaagtgcag aaagtgtgat
    14281 agccaatcca ttttgaacac cggaaacaat agatagagat tctaaggacg gaatgactcc
    14341 ttgagttttt aaccaatttg ctgcagtagt caattgaaaa tttgtgcctt tgggatcttg
    14401 gtaattaagc aaaccaacaa gatttgggtt attactagtt gattgaataa acgggacaac
    14461 gaatttatta cattcttcaa aagaactaac cagaccaaaa tcaatgatat gttcggttac
    14521 ttcattggta gagatggtga tggactcaaa gacattggga gaaataaatg tgccacttcc
    14581 aatattcgta tacagcaaac ctttctcaat tccatattta taagccttgc caacggtagt
    14641 aaaatttaaa tctaaataat cagctagctc tctttgggag ggtaactgag tgtttttctg
    14701 taatttattc tttaaaatat cttgttctaa ttgttgaatc aacgatcgat aataaggacg
    14761 cttcaatttt tgtttgtctg gtcgccaagt taacgggtaa ttgtcataag aattaatagg
    14821 cattttctac actccttaaa attgtatacc atacaatttt accattgatt gtattgattt
    14881 tagaagttaa tcttttgtga ggagggaatt gtctatgaaa attattaatt ttgaggaagt
    14941 agtagcttct tttagttttg aagaagcgat tcaagtaatg aaaaaatgtt tttttgatta
    15001 tgaaagtggt aaaatcagcc aaaatcctcg attggtggaa attttacctg acggaaaaga
    15061 caaaaatatg tttgcgatga tgcctgctta tttgggggag aacagatatt ttggaacaaa
    15121 agtcattacc gcttttccgg ataatcatca gtatcattta ccttctcatt taggagagat
    15181 tttcttgttt gatgctcaaa acggactacc caaagcattg ataaacgcaa atgcagtcac
    15241 ctggattcga acagctgctg tatctgcgct ggcaacagat tacttagcaa atccgcatgc
    15301 agaaacactt gctttaattg gagctggtca acaagcattg tcgcatttga ctgcgatttc
    15361 taccattcgt cccatcacta cagtgtttgt ttacgatatt ttagaagaaa gacgaaatac
    15421 ttttatcgct aaagctaaaa aacagtatcc taaaataacg tttataaatg caatatcagt
    15481 agaagatgca gtacgtccag cggaaatcat ttgtacgttg acctctagta aagaggcatt
    15541 tcttcaaaaa gagtgggtaa gggaagacgc acatatcaat gctgtgggca catttactcc
    15601 tgatacaaga gagataagta catcgttgat ggaaacttct caaatctatg tagatgatta
    15661 ctcagctacc ttaacagaaa gcggggacct gcttatagcc atggcagaag gagatttaga
    15721 ggaatcttgt attgttggta cattaggcga attagtttct gagaaaaaaa cattttataa
    15781 aggagaatcg aggccgacga ttttcgacgc agttggtcta gctattgaag atctttgttg
    15841 tgcagaatat atttataaca aattagagaa aggggcttta aaatgaactt agattattac
    15901 gtagttgatg catttgcaga tgaggtgttc aaaggaaatc cagcagcggt ttatgtgcta
    15961 gaagaatggc tgccagaagg tacgatgcaa aaaattgcca ttgaaaacaa tttatctgaa
    16021 acagcattta cagtgaagaa aaaccaagaa tttgagctta gatggtttac gccagatcga
    16081 gaaatcgatc tttgtggtca cgcgacatta gcaacagcat ttgttttatt taactattac
    16141 aaaatacctg atgaaacgat taaattttct actcaaagcg gtaatctatt tgtgacaaga
    16201 attaaagatt attattacat ggattttcct agtattatgc cgaaaaaagt gccgatattg
    16261 actgaatatg aagaagcaat tggggctaag attaaagaag cctacttagc acgtgattta
    16321 tttttcgtat tagaagatga aaaaacggtg gctaaattgc agccagattt tacagctatc
    16381 aaaaattttg aattaggaat agagtcatcg tgacagcaga aagtgtgcaa aaagactttg
    16441 tttctagaac cttctttcca aaattaacaa tcaatgaaga tccagtatgt ggatcagcac
    16501 attctaatct tattccttat tgggcagaaa gaatgaataa aacaaaattg tcagcttatc
    16561 aaatgtcatc aaggggcggg gatctagaat gtgaattaaa agaagatcgt gtgataatag
    16621 gaggaaaagc agttttgttt tctaaaggta cctcgtttat ttgatttctt tcaaatgaat
    16681 taaaaataaa ttggtggaga gtggcagcct tatagctaca atggttgtta aaaaaagacg
    16741 caaacttttt caatttaatg aaaagtttgc gtcttttttt aattattggt gtttgttttt
    16801 tattcaatca agagtaaaat gccccatact aactatcctc ggatgacttc gattttataa
    16861 ccatcagggt caatgacaaa gtagtaagaa ggcgtagtgc caggtaatcc ttttaagtca
    16921 gtaatctcaa gacctgcagc tttatgttgc tcgtgtaatt tttctaaatc atcagctgca
    16981 atagcgatat gaccatagcc gtctcctaga tcatacgcac catgatcata gttgtaagtc
    17041 aactctaatt catagtcatc tccaggaagt gtcaaataaa caagtgtaaa tttgttttct
    17101 ggaaaatcac ggcggcggct ttcttcaaaa ccaaatgcct tttgataaaa ttcgattgat
    17161 gcttcaagat ctttcacccg aacacatgtg tgagccattt tcataaattc atctctcctt
    17221 ttccttttac tcaatcataa caaaaagaag taagaaaata gttgatttgt gcttgttcat
    17281 gagaaatttc tcatgtaaaa tggatagaaa ataatactgt taaggtggaa acaattcatg
    17341 gataaaccaa tttttggtga aaaaaagcaa tctgctaatt atcaaacccg ttatgcagca
    17401 tatattgtca tagaaagaga caagcggatt gcggtgatcg aggctccaaa tggtgctttt
    17461 ttccttcctg gtggggaaat cgaaggagaa gagacgaaag aagaagcgat tgtccgtgag
    17521 ctgatagaag aaatgggaat tgtggcagaa atttcttctt atctgggaga agctgatgag
    17581 tatttttact cgaattatcg tgcaacttat tattataatc caggatattt ctttgttgct
    17641 gattcttgga agcgtatagg agaaccaact gaaaagacca ataaaatttg gtgggtgagc
    17701 cctcaagaag cgcttgtgaa attgaaacgt ggtagccacc gatgggcagt cgagagatgg
    17761 atcgaaaaaa ataaaaactg aaagaaagcg agtagatcag ctttctctca gtttttttat
    17821 ttattacagt acatattttt tgattgcttc agctacaccg tgctcgtcgt ttgatgaagt
    17881 gatgacattt gctgcttctt tgacaagaga aacggcattt cccattgcta ctcctagacc
    17941 agcatattca atcatggata agtcgttctc gttatctccg atagccatga tttcatcttg
    18001 atcaatacca aggtgctgtg cgagattcgc aactgccgca cctttacttg cttctttatt
    18061 tagtacttcg aagtaatagg gcgcactttt gaccgttgtg tatttatcac ggaaagtttg
    18121 cggtaaacgc gcaattgctg catctaaaat ttctggttcg tcgatcatca tcattttgac
    18181 gatgttcatg tctggtgtca tttcttcagg tgttcgatat tttagcggca tcttgactaa
    18241 acttgcttcg tggatcgtat aagcgctgat atctcgatta ggcgtgtaga tcgctttgtc
    18301 atcgatcgaa tggagatgac tgccgacacg gcgtgccatc acttcgatct ctaggaaatc
    18361 ttcatgagtc agtccgtgac gggcgatgat tttacctgat ttcgtttgtt gtacgagtgc
    18421 cccgttataa gtaataacgt aatcattatc ttgaaaaagg ttcaactcgt ttaactgttc
    18481 ctggacacct ggtaagggac ggccagtgca taaaacgatc gaaacacctt ttgcaacagc
    18541 agcggcaatc gcttctttta cttcttttgt tacttctctt ttctcattta ataaagtacc
    18601 atcgatgtcg atagcgacta gtttgataga cataatttcc tccaatatat tttaatttat
    18661 ttcaatcaat gcgccattac ggatatggct ggcaaattct tgataagttt cgtcaaataa
    18721 atcataatga tcacgcaagg agggatcaac catttctttt ggaaagaaaa atcgctcatc
    18781 tccttgttct tgtcccgcta atgcagcaac aagctgacta acctgtgata attctatcaa
    18841 agttccgtca tttcgcaaaa tctcgatttg tgtgcgatgt ctgccttgtt ctgggcgata
    18901 aaaatcataa ggcagatcgt agctagaatt cacagcagtg taataaacag ggttatagcc
    18961 aaccttttct actaacaaag tgagttcttg gactaatttt gaatcccggt tgccagaaaa
    19021 agtagcagat ttcagtggtt tacgattcaa aaaacgttta gctaaatcat ttaaaatagg
    19081 atcagcagat tcagaccatt gagtaaagta agtgcccaag accccatcat caagtttgag
    19141 gtattcttct aaagtaaagc ttccgtcaag aaatggcaga agtaattgag aatggagttc
    19201 aaaactgctg gcatcttctt gatacaactc gtgtgcgcga tgaagcagat gctccaaaat
    19261 cacttccata ccacgggaaa caggatggaa gtacacttgc acatacattt gatatcggct
    19321 aacgatataa tcttccactg catgcatgcc actcatggaa aaagcgatcc cgccttcata
    19381 aggacgaatc acacgtaata ttcgggtaag atcaaatgtt ccatattctg ttccagtgaa
    19441 ataagcatcc cgaagcaaat agtccattcg atctgcatcg atctgactag aaatcatttg
    19501 gacaacttgt gggttggggt aagttttttg gatcacacta gctacctttt caggaaaacc
    19561 ttcttctact cggttaagaa tctgatacac ttctgtttct ggagaagtga tgatctctac
    19621 agtgatggtt tcatgattcg tatggaagat atgttcaaat gtatgagagt aaggaccatg
    19681 accgacatca tgcaacagtg ccgcacataa agtcaccagc cgttcctgat catcccagcc
    19741 tccgttgcct attttttctt ttgaaaaatt tcgagaaaag atatcgcaga ttcgtcttga
    19801 aatctcataa acgcctaatg aatgggtaaa acggctatgt tctgctccgt gaaaagtgaa
    19861 agaagaagtg cctaattgtt taatgcgtcg taaacgttgg acttctttcg aattgatcaa
    19921 atctaagatt actttatgct ggacatggat atagttatgg actggatcac gaaaaacttt
    19981 ttccataggc agcatttgat atttataagg ggttgtcata atgtagctcc tttatgttat
    20041 tacgttgttc cattctctgg atttggttgt tccattgctc cagctggatt tcttttttta
    20101 gccagtcggt agaatcctgt aaatcacttg cagaatacag cttttcaaaa gcttttgcaa
    20161 atgcttcttt gacttctttt acaaaaaggg gttggtcaag cagttcttca agtgttgcca
    20221 ttgtagcagg atccactgca ggatagccat ttgtaccaaa gtcttctttt aaagcagatt
    20281 gataaaatcg cttcatgact tgcccgcgtt tttcctgatt accgttgaca cttaagtaca
    20341 tcataacaga aatcccattt ttgatccgac gctgagcgat accggcaaat tttttgccat
    20401 ggatgcttaa atcataagtt ccaggacaat aagaatgggt gatttcgaat gcttgtatcg
    20461 gtgcctttgg aaaagcgtat ttagtcaata ggagcatttg ctcgtaagcg tcatctatgg
    20521 ttattttttt tgtgtcactt tgagggaaaa tcaaagaaat atttaaaatt ccttcatctg
    20581 atacgatccc taatccacct gaatttctga tcactggagc gtaacctgca tctttcagtg
    20641 tttggatccc gtcacttaga aaaggagttc gaacatcctt catacctaag atgactgtgc
    20701 agtcatattg ccagaaatgt aaaaagattt gctggtgttc tttagaatat tccgttaata
    20761 tatctgttaa tgcaaaaggg gaaaagtcgt ttttttgata aatgtgctga tctagtcgaa
    20821 tcgcagggtt cattcaaccg gctcctttgt ataaattcat attttctaca atattatact
    20881 ctaaaatcaa agagttgttt tggaaaaaag gttttctgta aaaatggaca agttggttga
    20941 caaaacaaaa gaaactagga acaatgtagc tatgagaagt aaaggagaat gaataatgga
    21001 attatcacaa ggaacaccga tttcaatcaa attgcgtaca aaagtaaaac agcaagggga
    21061 agtgcaagat ttttattttg atttgaaagg gcaaatggtg acaattggcg atacgctgta
    21121 catacgttat aaagagattc aggaggagac gggcgatgag attcctgtaa cgatcaaatt
    21181 agttccagat ggtcatgtac agctgatccg tgcaggagaa atgcggatgc gtttgaaatt
    21241 tggctataaa gaacggttag aaacatccta tcgaacacca tacggtatga tccagattgc
    21301 tacttttacg aaagaactgc atgttagttt gaaagaccgt cccacagcag ggaaggttcg
    21361 aatcgattat gatctgttca tgggaccgga aaaaatcggc gaatattatt tgaccttgga
    21421 ttttacagcg taaatataaa agaaaagttt gattttttgt gtctgatttt gtatgataag
    21481 gagtagactg tgaaaggacg tgtcctgatt ggaaattagt gtatttgaag gtgcaaacaa
    21541 aagcgaatta tcaatgatcg aagtagcaca cgcgatctta gaacaacgtg gcgatgtcat
    21601 ggatttttct gatttagcta accagatcca aaactatctt gagaaatcag acagtgaaat
    21661 tcgtgattca ctcgcacaat tttatactga cttgaatatt gacggcagct ttatctcatt
    21721 aggggataat cgttggggct tgcgttcatg gtatgctatc gattcgatcg atgaagaagt
    21781 aaaccatgga atggacgaag aggatgaaga tactccacgt cgccgtaaac gcaagaaagt
    21841 caacgccttt atcaatgatg atgaagacgc gatcgattac aatgacgatg acccagaaga
    21901 tacagatctg acagaagaag atgacgatga tctgtttgat gacgatgatg atgaagatga
    21961 agaaatcgct gcttataatt ctgacttgca agaaatcgga gcagatgacc cgacagatga
    22021 tgaagaagac ttgccaggaa atatcgaaga agatttaagt atcatagacg aagacgatga
    22081 tgatgaagat tatgaagagg aagaattttc tgactctgaa aaagaataaa cattattttc
    22141 ttacgagagg ttgtgacaga gacgatcagc ctatcagaat aaggaggtgt gacacgattt
    22201 ttgtcacacc ttcttttttg tatataaaaa tcccaagtaa atacataaaa aataaaaaaa
    22261 ttgaaacttt tagcgcacct tgtacgtcat attaagtgag gcagaaagga gtggagaatg
    22321 atttttactg gatataagaa aaaacggcag atcgaaaaat accgtcacct gctgaatgga
    22381 ctcatcgaaa ctgattatca aaaaatgttc cgtatcacga tgaattacgt acatgataaa
    22441 gaagaggcct tagatgtgat gcaagatagt tttcataaag ccttgaacta ttttgaaaaa
    22501 gaaaaagaga tcgagcattt tagtgcatgg ttttatcgga tattgatccg cacggcatta
    22561 gatagttggc gcagacaaaa aaggacgcct gtggacttat ttgaaattca agatatcccc
    22621 caacagttac ctttagaaac ttccgttgca gagctccata tgattttaga taaaatagac
    22681 agtccggaaa aagagattct gatcctctac ttttttgaag gatttcgttt gaaagaaatt
    22741 gcaactatct tggatatgaa tgaaaacaca gttaaaacga aaatgtaccg ctcattgaat
    22801 gctttacgtc aaatcctaga atgaaggaga gaagagtgcc atgtattccc aaaaagaaaa
    22861 tttaaaaaaa ctaaagaaaa cttataaaga acaagcaatt cccaaagggc ttaaagagga
    22921 gatccgccag cgttttatta tcgaagagca atcatttata aaagcaaggc ggagaaagag
    22981 acgtctccaa gggttcggag tatctttggc tatcgtaatg attacaacaa gtagccttct
    23041 ttttaatagt caagttcgaa gctttgcaga agatttaccc atcttaggat cagtcattga
    23101 attgatctta ggcgaacgct ttactgatcg atctgaaaaa atcgatattc aggtaccgaa
    23161 gatcaataca caaaatgaga aagaaaataa aacaattcat ggattaaatc aaaaatactt
    23221 tcgcgaagga caagcagatt ttgaaaaagc tgaaaaagaa tatggaaatt ttgaaacgga
    23281 tcattaccaa gttttgggag attatcagaa aatcgtagac gataaccggt ttctcgtgat
    23341 tgaaaggcag atcacccaaa cagcagcaga tagccatgtt gaaaaaagat acgacacaat
    23401 cgacaagaaa aacagtgtcc agctctcact tccactttta ttcaaagatg acacttatct
    23461 atctgttttg acaaaagaag tcaaacggca gatggccgaa caagtcaagg aagatccatc
    23521 taagtactat tggactgagc aagatattca agaagggacg atagaaaaac caacacttgt
    23581 tacgcctaca cgaagttttt atttgaacaa agagcatcaa ttagttttga ctttttctca
    23641 atacgaaatt gctcctggtt atatgggtac accagaattt gtcattccta agtccgtgac
    23701 aaaaacgatt ttggcatcgg aagattattt ggatcattag acgagaagta gttgcgacta
    23761 gcagtttcaa ttttcaagta agtacaagta aataataaga agagaaaaag agaaagaaga
    23821 gatatagtga aaagaaatta tttagtcatc agtattattg ctttatccgt gcttttgttt
    23881 ttcgaaatta cttcggtggc ctccttgatc accgaagtaa aaggacaaac acactcttcg
    23941 gagcattcgt cacaatcttt caaacaatca gaacaatcaa ccattaaaag ccaatcaact
    24001 gaaacagcta acgtcaaaga agccattcaa gaatcagcgg aaaaggtaca aagtcaatca
    24061 tcacaatcaa cacaagacag tacagaagca atccaagacc cgactgtcaa aagcgtcgca
    24121 atcagctttg atgatggacc aggagcgacc acaacgccgc agcttctgcg aatcttaaaa
    24181 gaaaaaaacg tgcatgtcac ttttttcgtc ttaggagaaa atacggcgca gcatcctgaa
    24241 atcgtgaagc agacagctga agcagggcac gagattggca atcacacata cgatcatcag
    24301 aatctggcca ctctttctgc ccagtcgatg acggaggaag tgacgaaagc agacactgag
    24361 atcaaaaaaa gtcactggca aaacccccgc ttttgttcgt ccgccctatg gttctgtcac
    24421 gaatgtagga gcaacgatca tccagcgacc gatcatcgaa tggtctgttg attcagaaga
    24481 ttggaagaca aggaatcctg atttgatttt gcagaagatc caagcaacag tatacgatgg
    24541 gtcaattatt ttattccatg atatctatcc tgaaacgatt cgtgccgttc ctcaagtgat
    24601 cgattatctg aaagagcaag gctatcgcat cacgacagtt agcgatttat tagggcatcc
    24661 aacggctgtc gagaactatt atggcagaaa cgatcatcgg ccagttcagt aagtgagaca
    24721 agtatcttta ataaataaca cattttaata agtagttgaa cacataacta ccttacttgc
    24781 aaaggcttaa caatatctat acagatacaa caaaaaactg tccaccaatt ctctttggcg
    24841 gacagttttc acttaaaaat aaagaagcaa ctattcgtaa attatgctaa aatagcagta
    24901 gaacgcgtcc tgagggaatc gaacccccgt ctcaagaacc ggaatcttac gtgatatcca
    24961 ctacactaag gacgcaagca ctgggataat tgtaactaaa cagataaaaa attacaaggg
    25021 gattggacaa aaaaatgaaa tttgagcaaa atttttcaca aaaacagcag cagacacaga
    25081 aactagccat gacgcaacag ttgcaacagt ccatccaagt tttacaatat aacagtgagg
    25141 agctgttggc attcgttgag aatcaagcaa tggaaaatcc attggtggaa gttgttgagc
    25201 cggaatggca gccagattac ataaaagctt cttcatcttc ttatgaagga gaagagacaa
    25261 attacctaaa tcagattcct gatacaaaag ggtctttatt tgattcattg atcgaacaag
    25321 tccatttgaa ttaccgagat acttttttga ggaaaatcgt tctttatctt gtagaataca
    25381 tcgatttgaa cggatttttg acgattagtt tagaagaagc cggaaaagag attggagcta
    25441 cgcctatcca aatgctcgat gcgttgactc tgattcagca attagaacca gctggagtag
    25501 gtgcacgtag tttgcaagaa tgtctcatgc ttcaaacaga acgagatgat tatgcgccgg
    25561 aattagctta tatcgtgttg gaagaatgtt ttgaggaact ggtggagcga aaatggaaag
    25621 aaatcgcaca acgtttttct gttgatctac atgctgttca gcagatattc gactatttac
    25681 aaacgttgag tccaagccct ggccggattt tcgaccgtag cagcgaattg tacattattc
    25741 ctgatgtccg ggtattagtg gacgacgata aaaatgtaca agttatctca aaccgaaaaa
    25801 atcagccaaa tatacgcttt caagagagtt acttcaaaca aatgtcacaa caagcagata
    25861 aagaaactga aaattattta aaagaacgga aacaggaatt cgaatggttg aagaaaacga
    25921 tcctccaacg tggggataca attttgcggg ttgctcaagt cattgtttcc cggcaaaaag
    25981 ctttctttat cgacaaggaa cgtccaatca agcctcttac gttaaaggaa gttgcaactg
    26041 agatagatgt gcatgaatcc acggtcagtc gtgcagtcaa cgggaaatat ttggaaacag
    26101 atttcggtgt atttgaatta aaaaaatttt tcactacacg aattcctacg aatagtacag
    26161 aacagacaga agacctttca gcagatacag cgaagaaaaa gctgcaggaa ttagtcgatc
    26221 aagaagacaa aaacaagccg ttgtcggacc agaaattggt tgaattgctg aaaaaagatg
    26281 agatagcgat ctcaagacga acagtagcaa aatatcgtga tttattagga atcccgagtt
    26341 catcaaaaag aaaacgttat gataactaat cagtcaaaag actagaaaaa gtatttcact
    26401 ttttctagtc tttttagttg aaaaacgaga gcaaaactgt tagacttaca gtcgtgaggt
    26461 tgagatttcc ctttattgat gtgaaattcg aatagttaat ttcttttttt atcgtatatg
    26521 ggtcgtttta agtctacatt ggacgtaaag tgtccaacta aggagatcgc tatgctggat
    26581 gaaataaaaa tgattgaatc tgttgcacca gatattatcg aagtgttgca agaacgctat
    26641 aaaattttac gaaatatcta ttggatgcag ccgattgggc gtcgaagttt atcggaaagt
    26701 atggggatca cagaacgagt cttacgaaca gaaacagacc ttttgaaaaa tctgaatttg
    26761 atcgacacct caaaaagcgg tatgacgctg acaactaagg gcgaagaagt ttatcaaagc
    26821 cttgaaaact tcatggatca gcttttaggt acgcatcaaa ccgaacaaca gttggcagaa
    26881 tattttggta accaacgctg tatcgttgtt tcaggaaaca gcgaagagca gaccaaagtg
    26941 gctgatgcat tcggcgaagc gctgagcgaa gccttagacc gactacttcc agaaggcgag
    27001 aatatcatcg ctgtgatggg aggaactact atggctgtgg tagccgaaca attgtctaat
    27061 ctggaaaata agaaacggca taacctgttt gtccctgcaa gaggcgggat tggtgaagcg
    27121 ataactgtac aggcaaattc tgtcagtgca agaatggctg ctaaagcaaa cggtaaccac
    27181 cgcgcacttt atgttccgga acaattaagt ctggcaactt acaattcttt actcaatgag
    27241 ccatctatcc aagaagtctt aaacttgatt agtgaagcga attgtgttat tcatagtatt
    27301 gggcgtgcat tacacatggc agcacgtcgt aagatgactg aaaaagaatt agtcatgctg
    27361 aaacaagcaa atgctgttgc ggaatctttt ggctactttt tcaatgaaaa aggtgaagtc
    27421 gtttataagg tccctagaat cggcatagaa ttgcaagatt tggaaaaagt cccgatcatc
    27481 atggcaatcg cgggcggaaa atcaaaagct aaagcaattc gagcttatat gaaaaatgca
    27541 ccaaaacaaa cgtggctcat cactgatgaa gctgctgcaa atgagatttt aaaaggggta
    27601 accctttaaa ataaatattt ttttgatttt cataaggagg aaatcttaaa tgacagttaa
    27661 agtaggtatt aatggttttg gacgtattgg acgtctagca ttccgtcgta tccaagatgt
    27721 agatggaatc gaagtagtag caatcaacga cttgacagat gctaaaatgt tggcacactt
    27781 gttaaaatac gacacaactc aaggacgttt caacggaaca gttgaagttc atgaaggttc
    27841 attcaacgta aacggaaaag aagttaaagt attagctaac cgcaacccag aagaattacc
    27901 atggggcgaa ctaggcgtag atatcgttct agaatgtact ggtttcttca cttctaaatc
    27961 agcagctgaa aaacatttaa cagctggtgc taaacgcgtt gttatctctg ctcctggcgg
    28021 aaacgatgtt ccaacaatcg tttataacac aaaccacgaa acattaactg gtaaagaaac
    28081 agttatctca ggtgcttcat gtactactaa ctgtttagct cctatggcta aaacattgaa
    28141 cgacaaattt ggtgttgttg aaggattaat gacaactatc cacgcttaca caggtgacca
    28201 aatgactcta gacggacctc atcctaaagg tgacttccgc cgtgcacgcg ctgctgctgc
    28261 aaacatcgtt cctaactcaa caggtgctgc taaagctatc ggtttagtaa ttcccgaatt
    28321 gaacggtaaa ttagacggag ctgctcaacg tgttcctgta ccaacaggtt cattaacaga
    28381 attagtaaca gttcttgaaa aagaagtaac tgttgacgaa atcaatgcag caatgaaaga
    28441 agcttcaaac gaatcttacg gatacaacac agacgaaatc gtttcctcag atatcgttgg
    28501 tatgacttac ggttcattat ttgatgctac acaaactaaa gtaatgacag ttggcgacaa
    28561 acaattagtt aaaactgttg cttggtacga caacgaaatg tcatacactg cacaattagt
    28621 acgtacttta gaatacttcg ctaacttata agatagacaa acgtacaagt gtaaattata
    28681 gacatgtaca tttaaaagcg gggaagcaat cgcgcttctt ccgctttttt tgataataag
    28741 tttattcagg aggaaataaa atggctaaga aaacagtgaa agatatcgat ttaaaagaca
    28801 aaaaagttct tgtccgtgtt gacttcaacg ttcctttgaa agacggcgtg atcactgacg
    28861 acactcgtat caaagcagca ttgccaacaa tcaactacgt tctagaacaa ggcggaaaag
    28921 caatcctttt ctctcacctt ggacgtgtaa aaacagaaga agataaagaa ggaaaatctt
    28981 tagctccagt tgcaaaacgt ctaggcgaat tattaggtaa agaagtaaca ttcgtacctg
    29041 aaactcgcgg agaacaatta gaagaagcaa tccgcaacat gaatgacggc gacgttgtcg
    29101 tattcgaaaa tacacgtttt gaagatattg acggtaaaaa agaaagcaaa aatgatacag
    29161 aactaggtaa atactgggct tcattaggcg atgtatttgt taacgatgct ttcggtacag
    29221 ctcaccgtgc acatgcttct aacgtaggta tcgcttcaac tggtatccca acagttgctg
    29281 gattcttgat ggaaaaagaa atcaagttca tcggtgaagc agtagaagaa ccaaaacgcc
    29341 caatgattgc aatccttggt ggcgcaaaag tttctgataa aatcggcgta atcgaaaact
    29401 tgattcctaa agcagataaa atcttaatcg gtggcggaat gacttacaca ttctacgaag
    29461 caaaaggaat caagatcggt aactctctag tagaagctga taaagtagaa ctagcaaaag
    29521 aattaatcga aaaagcaggc gacaaacttg tcttgccaat cgacaatgtc tgtgcaccag
    29581 aattttcaaa tgatgttgaa acacaagtaa tcgaaggaga cattcctgat ggtttgatgg
    29641 ctttagacat cggcccagca tcagtaaaat tatttgctga tacattacaa ggtgcaaaaa
    29701 cagttgtttg gaacggacct atgggtgtat ttgaaatgag taactttgct aaaggtacaa
    29761 tcggtgtttg tgaagcaatc gctaacttag aaggcgcaac aacaatcatc ggtggcggag
    29821 actcagctgc agcagcagaa caattaggat tcgctgacaa attcacacac atctcaacag
    29881 gtggtggcgc aagcttggaa ttgcttgaag gtaaagaatt accaggacta gcagcaatca
    29941 acgataaata aacgaccaag tttgctcaat tctgacgagt aatcgatcaa aaggtgaagg
    30001 gagttttcat gactccctag ccatttttca ataaattaag gagtgtgttc ctcaatgcgt
    30061 aaacctatta tcgcaggtaa ctggaaaatg aacaaaactg catcagaagc aaaagaattt
    30121 gctgaagcag taaaaaacaa aatcccttca aatgacaaag ttgattcagt catcggatct
    30181 cctgcattat tcttacaaga attaatgaca tcagctgaag gtacagaact aaaaatctca
    30241 gcacaaaact gctactggga aaactcaggt gcgttcactg gtgaaacttc accagcagct
    30301 cttgctgatc taggtgttca ttatgtgatc atcggacatt cagaacgtcg tgaatatttc
    30361 catgaaacag acgaagacat caataaaaaa gcaaaagcta tctttgcaaa tggtatgaca
    30421 ccaatcctat gctgcggcga atctttagaa acctatgaag caggcaaaac tgctgaatgg
    30481 atcgaaggac aaattacagc tggtttagct gatctttcag ctgaacaagt gtcaaacatg
    30541 gttattgctt atgaaccaat ctgggcaatc ggtactggta aatcagctga cgcacaaatc
    30601 gctgacgaaa tttgtggagt tgtacgtcaa acagttgaaa aactttatgg aaaagaagta
    30661 tcagaagcag ttcgtatcca atacggcggt tctgtaaaac cagaaaatat tgctgaatac
    30721 atggcaaaag aaaatgttga cggtgcttta gtcggtggcg caagcttgca agcagattca
    30781 ttcttgtcat tgttagacgc tgtaaaataa ttgttgaaaa tttgtgaaaa atgcaagaaa
    30841 tgtttgcact aatgagcaaa attaactaaa atcgtatatg agggaaatcc cttaggaaaa
    30901 aacaaactca aaggagagac aaaacatgtc aattattact gatgtttacg ctcgcgagat
    30961 cttagactca cgtggtaacc caacaatcga agtagaagta tacacagaaa gcggagcttt
    31021 tggccgcgga atggttcctt caggtgcttc aactggtgaa tacgaagcag ttgaattacg
    31081 tgacggagac aaagctcgtt atggcggtaa aggtgtaact aaagctgtcg acaacgtaaa
    31141 caacatcatt gctgaagcaa tcattggcta cgatgttcgt gaccaaatgg ctatcgacaa
    31201 agctatgatc gctttagacg gaactcctaa caaaggtaaa ttaggtgcga acgctatttt
    31261 aggtgtttct atcgctgttg cacgtgcagc tgctgactac ctagaagtac ctttatacca
    31321 ctacctaggc ggattcaaca caaaagtatt gccaacacca atgatgaaca tcatcaacgg
    31381 cggatcacat gctgataact caatcgactt ccaagaattc atgatcatgc ctgttggcgc
    31441 tccaacattc aaagaagctc tacgtatggg tgctgaagta ttccacgcat tagcttcaat
    31501 cttgaaagct cgcggattag caacttctgt tggtgacgaa ggtggatttg ctccaaacct
    31561 tggttcaaac gaagaaggtt ttgaagtaat catcgaagca atcgaaaaag ctggctatgt
    31621 acctggtaaa gacgttgttc ttgctatgga tgctgcttct tcagaattct acgacaaaga
    31681 aaaaggtgtt tacgtactag ctgactcagg cgaaggcgaa aaaacaactg acgaaatgat
    31741 caaattctac gaagaattag tttctaaata tccaatcatc tcaatcgaag atggattaga
    31801 cgaaaacgac tgggacggat tcaagaaatt aactgacgta ttaggcgaca aagttcaatt
    31861 agttggtgac gacttgttcg taacaaacac tcaaaaatta tctgaaggta tcgaaaaagg
    31921 aattgctaac tcaatcctta tcaaagtgaa ccaaatcggt acattaacag aaactttcga
    31981 agctatcgaa atggctaaag aagctggcta cacagcagtt gtatctcacc gttctggtga
    32041 aacagaagat tcaacaatct ctgatatcgc tgttgcaaca aacgctggcc aaatcaaaac
    32101 tggttctcta tcacgtactg accgtattgc taaatacaac caattactac gtatcgaaga
    32161 ccaacttggt gaagttgcag aatacaaagg tttgaaatct ttctacaact taaaaaacaa
    32221 ataattcttt tctaatgaag atacatgatc gactattgaa agatagtcga gtatgatcac
    32281 ttagttaagt aatcgaactc tctacttgct gaaat
//
LOCUS       DAKBVG010000007        32286 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000007 DAKBVG010000000
VERSION     DAKBVG010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 32286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32286
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.007"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            <1..1152
                     /locus_tag="OUD09_000287"
     CDS             <1..1152
                     /locus_tag="OUD09_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HCT9691596.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="OUD09_000288"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="OUD09_000288"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCT9691597.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2728..3003
                     /locus_tag="OUD09_000289"
     CDS             2728..3003
                     /locus_tag="OUD09_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HCT9691598.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3009..3776
                     /locus_tag="OUD09_000290"
     CDS             3009..3776
                     /locus_tag="OUD09_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HCT9691599.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3944..5773
                     /gene="typA"
                     /locus_tag="OUD09_000291"
     CDS             3944..5773
                     /gene="typA"
                     /locus_tag="OUD09_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HCT9691600.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6034..7806
                     /gene="recQ"
                     /locus_tag="OUD09_000292"
     CDS             6034..7806
                     /gene="recQ"
                     /locus_tag="OUD09_000292"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HCT9691601.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            7920..8222
                     /locus_tag="OUD09_000293"
     CDS             7920..8222
                     /locus_tag="OUD09_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HCT9691602.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8225..9388
                     /locus_tag="OUD09_000294"
     CDS             8225..9388
                     /locus_tag="OUD09_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HCT9691603.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9414..12842
                     /locus_tag="OUD09_000295"
     CDS             9414..12842
                     /locus_tag="OUD09_000295"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HCT9691604.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            12863..13987
                     /locus_tag="OUD09_000296"
     CDS             12863..13987
                     /locus_tag="OUD09_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9691605.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            13977..14414
                     /locus_tag="OUD09_000297"
     CDS             13977..14414
                     /locus_tag="OUD09_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HCT9691606.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14398..14736
                     /locus_tag="OUD09_000298"
     CDS             14398..14736
                     /locus_tag="OUD09_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HCT9691607.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            14855..15415
                     /gene="rsmD"
                     /locus_tag="OUD09_000299"
     CDS             14855..15415
                     /gene="rsmD"
                     /locus_tag="OUD09_000299"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HCT9691608.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15412..15903
                     /gene="coaD"
                     /locus_tag="OUD09_000300"
     CDS             15412..15903
                     /gene="coaD"
                     /locus_tag="OUD09_000300"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCT9691609.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            15893..16936
                     /locus_tag="OUD09_000301"
     CDS             15893..16936
                     /locus_tag="OUD09_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HCT9691610.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            17095..17823
                     /locus_tag="OUD09_000302"
     CDS             17095..17823
                     /locus_tag="OUD09_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HCT9691611.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            17939..18184
                     /locus_tag="OUD09_000303"
     CDS             17939..18184
                     /locus_tag="OUD09_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HCT9691612.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            18181..18399
                     /locus_tag="OUD09_000304"
     CDS             18181..18399
                     /locus_tag="OUD09_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCT9691613.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            18535..19074
                     /locus_tag="OUD09_000305"
     CDS             18535..19074
                     /locus_tag="OUD09_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HCT9691614.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            19067..21340
                     /locus_tag="OUD09_000306"
                     /pseudo
     CDS             19067..21340
                     /locus_tag="OUD09_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
     gene            21378..22424
                     /gene="holA"
                     /locus_tag="OUD09_000307"
     CDS             21378..22424
                     /gene="holA"
                     /locus_tag="OUD09_000307"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCT9691615.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(22513..22767)
                     /locus_tag="OUD09_000308"
     CDS             complement(22513..22767)
                     /locus_tag="OUD09_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCT9691616.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(23167..23436)
                     /locus_tag="OUD09_000309"
     CDS             complement(23167..23436)
                     /locus_tag="OUD09_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9691617.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(23906..24361)
                     /locus_tag="OUD09_000310"
     CDS             complement(23906..24361)
                     /locus_tag="OUD09_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HCT9691618.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(24499..26427)
                     /locus_tag="OUD09_000311"
     CDS             complement(24499..26427)
                     /locus_tag="OUD09_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HCT9691619.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(26624..27457)
                     /locus_tag="OUD09_000312"
     CDS             complement(26624..27457)
                     /locus_tag="OUD09_000312"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCT9691620.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(27649..28353)
                     /locus_tag="OUD09_000313"
     CDS             complement(27649..28353)
                     /locus_tag="OUD09_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCT9691621.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(28376..29002)
                     /locus_tag="OUD09_000314"
     CDS             complement(28376..29002)
                     /locus_tag="OUD09_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCT9691622.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            29328..31703
                     /locus_tag="OUD09_000315"
     CDS             29328..31703
                     /locus_tag="OUD09_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HCT9691623.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(31727..32218)
                     /locus_tag="OUD09_000316"
     CDS             complement(31727..32218)
                     /locus_tag="OUD09_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HCT9691624.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
BASE COUNT        10693 a         5670 c         6708 g         9215 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
     2641 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
     2701 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
     2761 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
     2821 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
     2881 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
     2941 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
     3001 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
     3061 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
     3121 taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc
     3181 cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa
     3241 gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt
     3301 tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg
     3361 tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc
     3421 cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc
     3481 ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta
     3541 tgtacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata
     3601 tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta
     3661 tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag
     3721 cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa
     3781 aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa
     3841 atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa
     3901 tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga
     3961 tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga
     4021 actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga
     4081 ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga
     4141 ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga
     4201 agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg
     4261 aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat
     4321 cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt
     4381 tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta
     4441 tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac
     4501 catggcacct atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga
     4561 cgaaccatta caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg
     4621 aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt
     4681 agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg
     4741 tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat
     4801 ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat
     4861 tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga
     4921 aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga
     4981 tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg
     5041 agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt
     5101 atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg
     5161 cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg
     5221 taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt
     5281 aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta
     5341 cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg
     5401 acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat
     5461 gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat
     5521 gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca
     5581 aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca
     5641 attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc
     5701 agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa
     5761 aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct
     5821 ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa tggacataaa
     5881 ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa aaacttattt ctagagagtt
     5941 tttgtttcat aataagataa tgtttcacga aaggagatca acttgtcctt cgaagaaaat
     6001 cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg acttattaaa acaatacttt
     6061 ggctatgatg agtttagacc tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa
     6121 aatgtattag gaatcatgcc taccggaagt gggaaatcaa tctgttacca gcttccagct
     6181 ttactattag atgggcttac tgtggttgtg tcccctttaa tctctttgat gaaggatcag
     6241 gtggacgcgg cgaatcaatt aggtataccc gccactttta tcaatagctc gttagatggt
     6301 tacgaaacag ctagacgctt ccaagaaatc gatagacagc agtaccgttt actttatatc
     6361 gctcctgaac gttttatcat gccggatttt atccaagcaa tgaagcgatg gaacgtccgt
     6421 atgatcgcta ttgacgaggc acattgtatc tctcagtggg ggcatgattt ccgcccaagt
     6481 tatttacaga tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg
     6541 acagctacag cgactgtgca agtagctgct gacataaaaa gacttttaaa aattccagaa
     6601 aacaaccata ttcagactgg ttttgaacga gaaaatctac gatttcaagt aattaaagat
     6661 caaaaaaaag aacaatatct aatcgaatat ttaaagatca acaaaaatca atctggtatc
     6721 atttatgcag caacgagaaa agaagtagac cgtctctatc atttgcttaa aaaattcgat
     6781 ttttccgtgg gtagatatca tggtggtcta aatgagaatg agcgaacaga aatgcaagaa
     6841 gcctttttat acgatcgctt gcagctgatt gtagcgacaa atgcatttgg tatgggtata
     6901 aataaaagca atgtccgttt tgttatccat tatcaaattc ctggctctct agaagcatac
     6961 tatcaggaag ctggtcgggc gggaagagat ggtctttcca gtgaagccat cctgcttttc
     7021 tctcctcaag atatccaagt ccaaaagttt tttatccagc agtcccaaag agaagaaggg
     7081 caaaagcaaa aagaatatga aaaattaaaa gcgatgacag aatacgtgta tattgagtcc
     7141 tgtcttcagc aatacatcct aaactatttt ggcgagacaa gcagtccgtg caaccgttgt
     7201 ggtaactgtt tagatgacag agaaatagta gaagtaacga cagaagcaca gatggttctt
     7261 tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa tgctgatgaa agtcttagca
     7321 ggatcgaaag aacaaaagct gcaagcttta ggttttggac atttatctac ctatggactg
     7381 atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg aatatttgat atcgaatggc
     7441 tatcttctga ccatcaatgg tgagtatcct gttttgaaag taacagaaag agggatacaa
     7501 gtattgaaag gacaagaatc tgtttatcga aaggaaccca agaaagtcca gcagctatcg
     7561 gatgaagaaa ccgatacact atttgaagtg ctgcgggaac tgcgaacgga tctagcatca
     7621 gaagcaggcg ttcccccgta tgtcgtattt tcagattcca cattgaaaga aatgagccga
     7681 atacgtccat caagcagatt ggagatgctt cagataaaag gagttggcca aagcaagctt
     7741 gataaatatg gcgaagcctt tttatctcgg ataaaaaatg cggactctaa cgttctggat
     7801 aagtagaaag aacgaatacg taaaaatgaa taaaaacaac tggttaaatc tgtttttttt
     7861 gctccttatg aaaatcaaat atgttataat taaggaatta gaatgggagg taagcacaga
     7921 tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa tgatgaagca gaacggatca
     7981 aacgattaat ccgaaatcaa aacaacagtc tctgtatctc acaatgcaaa gccttcgaag
     8041 aagtggtaga tacacagatg tacggctttt cccggcaagt cggttttgct gtccgtatag
     8101 gagtaatcgg aaagaacgag ggccagctga tgttaagtga gctagaacga gaattgaatc
     8161 agttatatag tgaggtttac caagaaattt atgataaaaa agaagttggc aaggaggaat
     8221 agacttgcct aagaaagtga aaaaaaggca tctattagac tatagcatct tgattcccta
     8281 tttaatttta tgtgtattgg gattgatcat ggtatatagt tctacgtcgt atctattatt
     8341 agaaaacgga caaaatccgt ccgcatcagt cattaaccag agtatttttt gggtgcttag
     8401 cttgattgct atcgcactac tttataaaat gaaaacagat gttttgaaaa accagcgttt
     8461 gatcatggca gccatcgcag ttttgacgat tttgctgctg attgttgtgt tttttggtaa
     8521 ggagatcaat ggggcgaaag gttggctgca aattgcagga ttctcgatcc aacctgccga
     8581 atacctaaaa atcatttcta tctggtacct gtctctgact ttatctaaaa gacaaaacag
     8641 tgtacaaaaa gattttcttg ggactgtgaa acgtccactt gcaatggtta tcggacttac
     8701 tgcattagtc gctattcttc ccgattttgg taatgcggca gttatctttt tgattatttt
     8761 ggttctgctt ttagccagcg gagtcaatta tgtgtatact ttgattgtag gagtaggagg
     8821 cttttgtcta agtactttta cgatctggct gatcaatatc acaaacggaa agatcttacc
     8881 cggacgttta cagtatatct ataatcgatt tgctatttac caaaatcctt tctcagacga
     8941 attaaataaa gggcatcaac tagtcaacgg ctactatgcc atgttcaacg gaggactatt
     9001 tggcagggga ttaggtaaca gtatccagaa aaaaggattt ttacaagaag cacaaaccga
     9061 ttttatttat gcaatcgtag tggaagaact gggagtgatt atggggattt tgatattagc
     9121 gttgctattc tttatgattg tgcgcattgt ccttgtaggt atccgttcaa aagatccgtt
     9181 caattcgctt ttgtgcattg ggatcggcgc aatgtttttg atccaagtat ttgtcaatct
     9241 tggaggaatt actggcgtta ttcctttgac agggatcacg ttcccatttt taagtcaagg
     9301 aggctccagt ttgctgatgc tttcgatttg tgtcggcttt gtcctcaaca tcagtgcgga
     9361 tgaaaaacgc aagagcttag gtttataata aaaaaagatg taggagtggt tcgatgaaaa
     9421 aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt atttcgtgct tgtacagaat
     9481 taggaattaa aactgttgga atctatgcag aagaagatga atattccgta caccgtttca
     9541 aagcggatga agcatattta gtaggacaag gaaaaaaacc gattgatgcg tatctagata
     9601 ttgaagggat catttctatc gcgaaagaat gcggagctga cgctattcat ccgggttacg
     9661 gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga agaagaaggc atcatttttg
     9721 ttggacccaa actgcatcat ttagatattt ttggagataa aatcaaagca aaagctgcag
     9781 ctatcgaagc ggggattgct tcgattccag gcacagatgg accaatcgct tctatagacg
     9841 atgccttgga gtttgcgaaa caatacggtt atccgattat gatcaaggca gctctaggcg
     9901 gcggcggacg cggcatgcgt gtagctcacg atgaaaagag tgcaagagag ggctacgaac
     9961 gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga agtgtatgtt gaaaaataca
    10021 tcgctaatcc taagcatatc gaagtacaga ttctcgggga tacgcatgga aacgtcatcc
    10081 atctttttga aagagattgt tctgttcagc gccgtcacca aaaagtagta gaagtagcgc
    10141 cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg ccaggcagct gtccaattga
    10201 tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt tcttgttgaa ggagatgatt
    10261 tttactttat cgaagtcaac cctcgcgtcc aagtggaaca tacgatcaca gaaatgatta
    10321 cagatattga tatcgtaaca acgcaactgt tgattgctca aggtttggat ctgcataaag
    10381 aaatcggatt gcctcagcaa gaaggaatta aattaaatgg ttcagccatt caatgccgaa
    10441 tcactacaga agacccgttg aataactttt taccagacac agggaaaatc gatacgtatc
    10501 gttcacctgg tggttttggc gtacgtctgg atgtcggaaa tgcttacgcg ggctatgtag
    10561 tgacaccgta ttttgattca ttgctagtaa aagtatgtac gcatggtgct acctttgaaa
    10621 cagccattca gaaaatggaa cgttgcttga aagaatttag aatccgtgga gtgaaaacaa
    10681 acattccatt tatgctcaat gtcatcacac atcctgaatt ccaatcagga aatgcaaaaa
    10741 caaccttcat tgatagtaca gcaacattat ttgaatttcc tcgactacga gaccgcggaa
    10801 acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg ttttccaggt atcgaaagtg
    10861 gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga tctaatcaca agaactgatt
    10921 acgtaacggc taagaatgta ttagatgcga agggtgccga tgctttggtc gaatggatca
    10981 aaggacagga aaatttgttg ttgacggata cgacatttag agacgcccat caaagtctgc
    11041 tagcaactcg tgttcggaca aaggatttca agcagattgc acgtttgact ggagaaggtc
    11101 ttcctgaact cttttcaagt gagatgtggg ggggagcaac atttgatgtg gcttaccgct
    11161 ttttgaatga agatccttgg caacggttga gaaaaattcg ttctttaatg cctaatacgt
    11221 tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta ttctaattat cctgacaatg
    11281 tattggtgga atttgtcaaa gaagcagcag cgcaaggaat cgatgtattt cgaatctttg
    11341 atagtttgaa ctggactcca caaatggaaa aaagtatcca agctgtacgg gatactggta
    11401 aaatcgcaga ggcagctatt tgctatactg gcgatatcaa cgatccaagt cgggcaaaat
    11461 acaatgttca gtattataaa gacatggcaa aggaactaga acaactgggt gcgcatatca
    11521 ttgcaatcaa agatatggct gggctgctta aaccgcaggc agcttatcgt ttgattagtg
    11581 aattgaaaga aacgacagat ctgccaatcc atctgcatac tcatgatacc agcgggaatg
    11641 gaatcatcac ttattctgct gcttcaaaag caggagtaga catcgttgac gtggcaatga
    11701 gtgcaatgag tgggaatact agtcagccta gcatgagcag cttgtattat gcactggtca
    11761 atggtccacg gctgccagaa atcacgattg aaaatgcaca gaaattaaac cattattggg
    11821 aagatgtgcg catgtattac aaaccgtttg aaaacggctt gaatgctcct gaaacagaag
    11881 tatatatgca tgaaatgcca ggaggacagt attcgaatct acagcaacag gcaaaagctg
    11941 tcggattagg tcatcgctgg gatgaaatca aacaaatgta tcatactgtc aacttgatgt
    12001 ttggcgatat cgttaaagta actccatcat ctaaagtagt tggagatatg gcactattta
    12061 tggtccaaaa tgatcttaca gaagaagata tttatgaaaa aggcgaaaca ctaagtttcc
    12121 ctgaatctgt tgtgactttc ttccaaggag aactagggca acccgttggc ggatttccta
    12181 aaaaattgca aaagattatt cttaaaggca gacctgcatt gaatgaacgt ccaggtcttt
    12241 tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt ggcagaaaaa attggctatg
    12301 aaccaaaaca agacgaagta ttaagctatt tgatgtatcc gcaagtcttt ttggactatc
    12361 aaaaagcata taatcaattt ggcgatgtta cattactgga cacaccaaca tttttccaag
    12421 gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg cggaaaaacg cttatcattc
    12481 gtttagatga aatcggagaa cccgatatcg aagggaatcg cgtactgttc ttcaatttga
    12541 atggacaaag acgtgaaatc actatcaaag ataattcgat catcagtacg gtccaaaccc
    12601 gattgaaagc tgaaccgaca aaccgagaac aaattggagc aacgatgtct ggttctgttt
    12661 tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg tgatacgttg atgattactg
    12721 aagcgatgaa aatggaaacg gccatcgaag cacgttttga cggagaagtc gcacatgtat
    12781 atgtgtcttc aggagacact atttcgtctg gtgacctttt gattgaagtg actgagaaat
    12841 aataagcgaa aggtggggaa acatgaaaag actgggtttg ttttggctga ttctgctatt
    12901 agttattgtg gcattctaca tggaaccagt cttgtttccg ccaaaacttg aagtgaagga
    12961 aaactctgat caacaaatat caaaccaaat gacaacgtgg aaataccagg agcttaaagc
    13021 aactggtttt tctacttata tcggaaaacc gatcgatcag ctggaaaagg attttggcca
    13081 agcctatgat cgattaacta gcggatttgg ttttgaaaca agatattatg aaaataaagc
    13141 tagtcatctc acatttgaag cgaacatcca agacagcaga gtcacaactg ttaaagtact
    13201 gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg atgacgatgc aagatcttac
    13261 gaaaatcact actatctata ctaatttcac ttttgagtac catgatacag aagttggaat
    13321 cgaactaatg gaagaggata tgaattatcg tccgctaatt gcttttgata acgacacttt
    13381 tgctatttta ttttttgatc aaacaacaaa cggcctattt tcagttgtgt atttagataa
    13441 agatagtctt ttgaaactat tgccatatca agtgtttggt gaaggccttc cacattatca
    13501 agcagataaa tcggctgatt gggaatcaat caatcgcgtt aaagaacgaa aaagtacgac
    13561 tttattaaat atgcttagaa gagaagacga acttcccata tacaatcaaa cacttgcctt
    13621 tcaaaaccac agtcagctgc tactacatga ttttttgaca aggcctgagg aaatattgtc
    13681 agaggaacgt atggctgatt ggcaaaaatc gctaggcagt gctcaaacga cagtgaaatt
    13741 ctcactgacg aatgatgaat tggagaagtt aatcagtaaa caaaaattag aaaatactag
    13801 tgggctattt attcatccgg cgattgacgc tactttttcg tttttatatt ggtacagcga
    13861 tccttacaac catgaccgtt tcatggaccc gggaacggat tctgtaggca ttgctttttc
    13921 taaagaaaat atgttagtct tattgcaaga agaaaacgga gaaagtagtg atagcagtga
    13981 tcgttaacga agagctgttt caactggagg atcaatgcct ccaagtcgct gaaatgataa
    14041 aaaagagtaa aacgatggct tcttatcttg aacataaaca gtcaatggaa gaatctgcaa
    14101 aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga agcatttgaa aatgtagcgg
    14161 catatggtga gtatgcacct gattatcgtg aaaaaaaacg agctgttcga aaagcaaaaa
    14221 gacagcttga tatggatgag catgttgcag ctttccgata tagtgaaaca cagctgcaaa
    14281 gtctgttgga tcaaattgtc caagtacttg ccgaagaagt ttctaatgaa atcaaagtag
    14341 atgcaggtag cccgtttttt gaaacgaaaa aacactttgg atgcggaggt aattgtcatg
    14401 caattagaca atagtgaatt tgaaataaca aaacgacgcg ggctgatcgt atgggtctac
    14461 agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt tgcattatgt ctcaagaaga
    14521 atgaaatatg tcgtgcttta cttaaatgaa gagacattgg aagaaacaga agaacgaatc
    14581 cgtaaattac attttgttcg aaacgtagaa cgttcttacc gaccggatat cgaaatgaat
    14641 tttgccgaaa aaataggaaa caaaacagta ttaaaagacg aaggtttcga aattgaagag
    14701 ctaaatacag aaatccgatt agctgaacat ctttaaaaat ctcttatgag cagtttttaa
    14761 ataaaaaata acttggataa cgatcgattt ttgatcattg tccaagtatt tttttggtat
    14821 actagtacca gtaccatttc taaggagtga gtagatgcgg ataatatcag gaaattatgg
    14881 cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga ccaactacag ataaagtaaa
    14941 agaatccata ttcaatatga ttggaccata ttttgatgga gagacagtat tggatctgtt
    15001 tgcaggaagc ggtggtttag ctatcgaagc agtatctaga ggctgcagtc atgcggtatg
    15061 cgtagacaaa aattaccaag cgctaaaaat catcaaagaa aacatcgaaa ttacgaaaga
    15121 accagagaaa tttaccacaa taaaacttga tgcggacaaa gcaatcgaac ggctagcagc
    15181 ggagaaacaa caattcgact acttgtttct tgatccgcca tatgcaaaac aaaaaataat
    15241 cgatcaaatc cataaaatgg aagaactggg acttttttct aaaaccgcag ttatcgtatg
    15301 cgaaacggat aaaacagtcg aattgccaga aactgtggct ggattccatc aaataagaaa
    15361 acagatttat ggcataacgg ccgtaactat ctatagaaaa gagggaaatc aatgaaacgt
    15421 gcattattcc caggaagttt tgatcctttt acaaaaggtc acttagatac ggtggaacgt
    15481 gcggctaaat tattcgatga ggtagtaatt ggtgttttta tcaatacttc aaagaaaagt
    15541 ttgtttcccc cagaagaaag aatgacattg atcacaaaag ctgtttctca tcttcctaat
    15601 gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa cagcaaaaga gattggtgca
    15661 gatgcgttga tcagaggcat acgaagtatc aaagactttg aatatgaacg agagattgct
    15721 cagatgaatc atcatctata tgatgaattg gaaactgtct tcttattagc aaaaccagaa
    15781 tatagtcacg tgagttccag tattttaaaa gaagtactgc actttggcgg agatgtgagc
    15841 agctacttac cgccagtgat caacgaagct ttagctggaa agcgtgaatc aaatgaaagc
    15901 taaacaatgg ttgaaaacag tactgattat catatttgga atgttgatca ttggatttgt
    15961 agtagttccc ttgccgtatt atgtagaatc accgggagcc acgatcgatc tgaaagaact
    16021 aattacggtc aatgataagg aagataaagg ttctgggtct ttctctctta cctctgttgg
    16081 tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa atgacccctt ttgaagaatt
    16141 gatcagtgaa gaagaattga ctggtggggc gacaaatgaa gaatataatc aaatgcaaca
    16201 gtattatatg gattcttccc aaaatgcagc aatcgaacaa gcgttaaaac ttgcagacaa
    16261 gccttataaa atggaattca aaggcgtata tgtcatgagt gtagagaaaa catcgaattt
    16321 ctttggcaag ctttcagttg gagatacagt aacaaaagta gacgggaaaa gctttcaaag
    16381 tacagaagca tttatggatt atgtaaaaag ccaaaaagtt ggacaaacaa tcgaaatcag
    16441 ttatttgcat aatggtgaag aaaaaacagc ttctggtgat ctgatcgagt taccaacaga
    16501 taaaaaggca ggaataggta tcacgcttac tgaccatacc gagatcgaat cagatacaga
    16561 tgttcgtatt aattctggct ctatcggtgg gccatcagcg ggattgatgt tcactttaga
    16621 aatctatgaa caagtcactg gtaaaaactt gcgacatggt aagcaaattg caggaactgg
    16681 aacgatcaac agtgaaggag aagttggccg aatcggagga atcgataaaa aagtggcaag
    16741 tgcggataaa gctggcgtag agattttctt tgcaccagat gatacgattt cagacgatgt
    16801 taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa gcaaaagctg ccgctaaaaa
    16861 attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta caagaagcgc ttgactatct
    16921 agagaaaatg aaatgaaact aaataattaa agaggggttg tgacacgagt tctgtcacaa
    16981 cccctctttt tcgtcaaaaa tcttcacgat aaaaataaaa acaaaagaaa aaattgacgt
    17041 aattagatgt tgaagggacg attttgccag agctcactgt ttcttcaatt ttaaatgaaa
    17101 ggagtaatga atgattttta ctggtcattc tttgaaaaaa gaacaatgat agaaaaaatc
    17161 agcaagaaaa tactagcttc acccaaattg atcatttgga tgtctatgat agttcttatc
    17221 ttttgtatcg caagtggttt tttattatca aatttgctat tttatagaga aacagaagag
    17281 actgctgcaa aagaaatact ttcttcgttt agttccagtg gagaagatcc atcagctgaa
    17341 ggaaacgatg tagaagagga tgctcgtcca aaggtcatgt acaccgatat caaaggaagt
    17401 gtgaaggagc ctggtatcta ttccttttct tcagaagaac gggtctatga tgtactaaag
    17461 cgtgcaggtg gattattaga agaagcggat agcgatcgaa tcaacttttc tgcgaagata
    17521 gaagatcagc aagtcctata tatccctgct gtaggtgaag agctgccaga acatcttgat
    17581 cagttggcat cctcggaagg aaaacagcct acagcagatt ccgagcctcc caaaataaat
    17641 atcaatacag ccactccatc agaacttcag cagattcctg ggatcggatc agtcaaagcg
    17701 caagaaatca ttcgatttag agaagaaaac ggatccttcc agaaagtgga agacctgcag
    17761 gagatttctg gtatcggtga aaagacagtt gaaaaactaa aaaacttcgt tacaattaaa
    17821 tagaatgatt caaaatcgac tggcacgagc aataaatgga cgctaatgga tactaagaat
    17881 aatccaattc taagagcttg gcaggacgag gaggtagtat ttttggatag gaaatagctt
    17941 gattgttcgc aaagaaacat taaaaaaacc aatgctgaat gtctatctcc aaaataaaat
    18001 atctgggatt catattatga atacggctgt cagcggcaat aacagtcaag ctctacgaga
    18061 acgctttgcc aaagatgtac ttagctatac agcagataaa gtatttattc tcattgggac
    18121 gaatgatcta gcagagcata aacaactttc taaagaaacg taccaaaaaa tctgcagtgg
    18181 atgatagttc agctgaaaaa agactatagt tctgatcatg ctttctttat cacgccacca
    18241 gcagtcgatg agacaaaaca aaaatatcgg aataacaagt tgatcaaaga atatatctct
    18301 gttctcacag aagtagcaaa acgccatgat tgtgaagtat cgatttttat acggaatttc
    18361 ttgaacaaat ggattcgaag aatttttata atgggctaat ggatgacgga cttcatttcg
    18421 gcagtgaagg gtaggatctt ttgtcagaca ggatgattcg ttatatttaa agaatatttt
    18481 gtttaagcaa tgagtacttt ttaatcaact tgaaaaaacg gaggaatata aaacatgtta
    18541 gaaaaatctt caaaagcatg cgatacgaat aaagaaaaag ttactgataa gtcacataat
    18601 gaacgcattc cttgggacca gtactttatg gctcaggcag tactgttatc tttacgtagt
    18661 acgtgtacac gtttagaagt aggagcgaca ctcgttcgag aaaaaaggat cattgctggc
    18721 ggctataatg gagcagtcag tggagatgtc cactgcatag atgaagggtg ttacgtcgtg
    18781 gatggacact gcttgcgaac gatccatgca gagatgaatg cactcctaca gtgtgcaaaa
    18841 cttggtatcc cgacggaagg ttcagaaatc tatgtcacac attttccatg tttggcctgt
    18901 acaaaggctt tgttgcaagc tggtgtgaaa gggatcaatt atctccatga ctatcgaaat
    18961 gatccttatg cccaagcgtt gatcgaacaa ttaggtgtat ctgtacacca agttgcttta
    19021 gactcacagc atttttcaaa acttcaaaat gaattaacag gaaaacatgt ctagcgtttt
    19081 agaaaggcat gttcgaaatc ggttgatttt tccggcaatt acgtcgatga ttatctgttt
    19141 gttgtattac actgacaatt ggggttatcg tttgcttctt gccggaattt ttttatggtt
    19201 ttcgctaaaa gaaggacgtt ttatcgcgta cttgtgtatt ttgtgtagca taacggtatc
    19261 catttcttat aggctacaag agtattcatt tttagaggaa aaaacaacat ctgcgcaaat
    19321 tactgtattt acagatacga ttcgtgcaaa tggtaatttt ttgacaatgg aaggaaggtt
    19381 atcagagaat aaacaaaagc tccagctttc ttatcaagta aacacagaag cagaattgaa
    19441 aacatggctt aacttccgag gaaagggaat agaattgact gctgaaggcg agttcatctc
    19501 tccagcaaaa aagcggaatc tttataattt tgaccaagaa gcttacttca ggaaccatag
    19561 aatttctgga cggttccaaa ttcaaaagat tgaacagatc accatgcaaa atcactggcg
    19621 aaactggctt cagagaaaaa gaggatcttt tatctccttt gtccaatctg aatttccgca
    19681 aaaaacaagt gtatatatta attctttatt atggggctat aaagatgcag gattcagaga
    19741 accagaagaa atttttaggg agagtggttt gctgcatctg ttcagtctat cgggtctaca
    19801 catccaattt tatttagggt gactgtatta tttatttcga cgtatcggat ggccactaca
    19861 cgtcagtatt attccgttga gtctactaac tatctgttat gtttctattg caggaagttc
    19921 tatttctgtt ttgcgtgcta gtttgctgtt tttattgcga ttgctagtca aattgctgaa
    19981 taagcattat tccaccatgg acttatttgc agttgtcttt tggcttcttt tactatttga
    20041 tcctcgattt ctttttcatg cgggtggaca gcttagtttt ttcatgagtt ttttgtttat
    20101 cctgatttct tttgatggag attggttgaa aaaaacggcc tctcacgtcc tgtttactat
    20161 gatcacgatg ccgttgattg tatggcattt ttatgagtgg cctattttag gtagtctttt
    20221 tacattactg attgcaccgg tattcaagtt tctttttctt cctggtgtgt tatttctcac
    20281 actcttcaat cgctggctgc cggaaacttt acttttgctg atagaagagg gattaatctt
    20341 atttgagcga ataatcgatt tttcttcagg gtcgtctctc attataggac gtttgcccct
    20401 tgtgctatct gggatgtata ttttcggatt actgatttgt atggataggt tgaaagaaca
    20461 acctgtgaaa cacttgtttt atgttggtct tttttctttt atttttttga ctttgccatt
    20521 tttacgcttt tcttcaacga tcgcttttat cgatgtcggt caaggagaca gtatcttttt
    20581 gcaaagtcct tttcgaaaag aaacgatttt gatcgatact ggaggtcagt tacactttga
    20641 aagagaaaag tgggcgcaag gtatgatacg tcctcctgct gaaagaaata tcgttccata
    20701 tctaaaaggt caggggataa gtgtaatcga taaactcatc ttgacacacg acgatacgga
    20761 tcatgtggga gaattaccca cgttatctca gcattttact atcaaaaaaa tttatatcgg
    20821 ttggggcgct ggacgaaatg agccattaca aaggcattta tctgaagctc aaaaaaatgg
    20881 tactgagatc attgaaatca aacatgggga caggatcagc ggttattatg atctttatgt
    20941 attgactcct tttgaaaagg gagagggaag aaatgaagat tcgattggtc tatggatgga
    21001 atataacaat aggcgctttt tatttttagg agatttgaat caagcaatgg aaaaacaatt
    21061 gcttttgatt tatcctaatc tgaaagccga tgtagtcaaa ttaggtcatc acggtagccg
    21121 tacgtcaagt aacacagatt ttctttccca tattgaagca aaacatggga ttatatcctg
    21181 cggcgtaaat aatcgttatg gtcacccgca cagcgaagta ttggagacac tagaagaaaa
    21241 tcagataaaa acatggcgaa cggatcaaca aggaatgatc acctatcgct ggcatccagt
    21301 ttttcatcct catgggctag tggaaacaat gatagactag taattctcat ctgctcatgg
    21361 taggataatt tttgaaagtg gtgagtaatt tgagtataca agcagagtta caaaaaatac
    21421 gtaaagaaaa acttgcatct tgttacttga ttttaggaac agaaaagttt ctccaagatc
    21481 aagttcggac agaaattgtc aaaaaaatcc aattagaggg cgaagatgac ttaaattttt
    21541 taagttttga catggaaaat gcaagtttag atgaggtagt agctgaagca gagacgttgc
    21601 cgtttttcgg agatcaacgg ttagtattcg tagagaatcc ttatttttta acaggtgaaa
    21661 aagtaaacaa tggaatcgaa caaaatactg atttattgac ggcttatttg aaagaaccat
    21721 tagaatcgac tgtattagta ttctttgcgc catatgaaaa gttggatgaa cggaaaaaag
    21781 taacgaaaca attgaaaaaa acagcaatta tcatcaatgt ccaacagttg aatgaaaaag
    21841 aagtacgtca atatctgatg aataccttag agaattcctc aatcaaaatg gaacgaagtg
    21901 caattgattt attcttgagg ttgaccgact tagatttatc caaaatgatg agagagttac
    21961 agaaattggt cctttatgga caaaatcaac agcaaatcac ggtaagagaa gtagaagaat
    22021 tagttcctaa aacgttagaa cataatattt ttgacatgac ccaatatatt ttaaaaggga
    22081 agacggaaca agctttgcgt ttatacgaag atttagttat gcaaggggaa gaaacaatca
    22141 aaattaatgc gattttgctt tctcaattac gtttattttt acagacaaaa tttcttgtaa
    22201 agattggtta tcagcaggca aatattgctg aaacattgaa aatccatcca tatcgtgtaa
    22261 aacttgccat gcaggaagta aaaaaattcg atgaacattt gctggtacag ttgtttgatc
    22321 aattagttga gatggattat caaatcaaaa cgggacaaat cgaaaaagaa ttaagttttc
    22381 aactattcgt tttgcgcaca gggcagttgt taagaaatcg ataagaagaa agacgagggc
    22441 aaagcatgcg ggaaggtcgt ttgtttttgt cctttttttg acaaataaaa aaatcggccg
    22501 aagccgattc ttttatttag cgattttttt gcttaaacga gatttgtcgc ggttagcttt
    22561 gtttttgtgg attagacctt ttgattcagc catatcaata gctttaacag cttctttata
    22621 taacgcatct acgttgtcag cacctgaagc tacagcatct tcaaattttt tgattgcagt
    22681 acgcatagcg cttgtttgag atgaattttt cacattagcg ttttcgcttg tacgaacacg
    22741 tttgattgca gattcaatgt ttggcattag tttcacctcc gataacttaa gttcttacgc
    22801 gtgttaccgc ataattcaac attcattatt atacctaaag gcagcgtgct ttgcaatatg
    22861 aacatgacaa gtttttttcc ttaacagtct tttattagga ttttttttca gtaaaaacgg
    22921 atagtacaag ctgaaaaaaa tcaaaaagac gctattgtgg gaggcaatag cgtcaaacca
    22981 aactagattt ttatttatct atttaaacaa agtttgaatg ggaatgtttg gtactaagtt
    23041 attatataac aaagagaacc gtttgcaaag ttttttttga aaagggatac gattccctgt
    23101 tgttctttgt ttttctatta agaaactgca gttattttat aaccagtatc ctttgatcag
    23161 taaaggctat tctggtgtac cgattatttt aatgtattca tcgatatttt ttatcaacat
    23221 tttttttatg accaactggt tccgacggtt ccatttgcca cttacttcca gcacatattt
    23281 atttaggggc aaacgtaaga tatattttgc caaatagcga tctttaagca ggccattgat
    23341 cacacggtcc tcaagctgca agttgatccc agtcagcatc attggctgtt cttgtaaaac
    23401 gtacgtatca attaaaagac cttcaaaagt ttccacaagc gtcttccttt cttttcaaag
    23461 taacctgaaa acaggcacta attcttccat tttgatattc gttgaagtgg atgcgatcat
    23521 tctttctttt gtccactgta atctagtata acaaaaaaga gcagaagagc caaaaagaaa
    23581 aaagagcatc gaattctata ggaagtctca ttggcacggc ctaaattatt acaaaacatt
    23641 gattaaaaaa agcccctgca tcgcaggagc atcccctata tcgttttaat aggtcaatca
    23701 aaaagcagta tcaaacacat tttgtatcaa atcaggaaat aatagataag ctaagcccag
    23761 aatcaaaagt accgctagaa gaaaccagcc gattgttcct atgaaatact ttaatttttc
    23821 gaacattgta tcacctcgtt gtcagtatat cacggatcag taaattgggg gtgttaagaa
    23881 tctcctaaaa tagacaaaat ttgttctatt ttcctaaagc aaatacttgt aagatacagt
    23941 aagtgatacc agaagcaatg accggtccgg cggcgattcc tcttaagaag acaacaccta
    24001 aaatcgtgcc gaaaacaagt gcaacagtga tttgtggatc agaagcaatc aatcctacac
    24061 ccttggaaga taagacagcc actagcccac cgcaaaaaac agctacccag ccaatagggg
    24121 atttaaaggc ttctaataga tcacgtagac cgatttctcc tgttgcgata gggattagaa
    24181 tagccgctgt gatgatcgtc accccaatat tgattccttt gccttgaagc caagggaaaa
    24241 aggaacggga ggctggtatg agcttcataa tcaaaacaaa agctgccgct atcatcagac
    24301 tttgattttt agctaaaatt gctacaagta aaataattcc taaaaaaagc caactttcca
    24361 tgaaatatat tcctcacttt cttcgaaaca atcataacat gtttttagaa agttacaaaa
    24421 gtaggaattc atccttcaag acctattcat aagctgtttt tttcataaaa taaacaaaat
    24481 cataaaaaaa cactttgctt atttgacaga agacagccgc gctttgtata gtggacaaga
    24541 cttacatttt tttgaagaat cagaaagatc aggtaaaggt cttagtaaac gcaagccatt
    24601 cagaataacc aatattgtgc ttccttcgtg tcctacaact ccaagaggta gatttaaaaa
    24661 ttgcaggaag ttagaagcaa tcaacaagag aataacaaga atcgaaaaaa taatattttg
    24721 tttgatgatc cgccgcattt ttaaagatag ccgatgactg tagacaaatt tatttaagtc
    24781 gcttttcatt aagacgatgt ctgcgatatc catagcggca tctgtccctt gccccatagc
    24841 gacacctaaa gaagcggctg ctaaagcagg cgcatcattg actccatcac caaccatcgc
    24901 atttacttgg taaagttcat tctctttttt gactagttgt gttttttctt ccggagtaca
    24961 gcttgcatag taatgttcaa tccctacttg ttcagcaatg gcctttgctg tgccagaatg
    25021 gtcgccggtt atcatacttg tatggatgtt ttgggattta aagtaggaaa ttacttgttg
    25081 ggtatttgct tttggaatat ctagcagacc taaaacagca attaactgct gatctttaga
    25141 aagataaatc acggttttgc cttgattttc caaacgttcg atcatttccg caatctcttt
    25201 gctgattatc atcgtctcag gatcatatgc gtgcttgcca actttccatt gcgcacctaa
    25261 ataggtagtt tgtaatccaa aaccagtaat ttcttctatt ggtaagtatt ggatttcttc
    25321 aggtattgtg caatcgaaat attgcaaaat cgcatgagca agtggatgcg tcgttttttg
    25381 ttccatagca acaagaagtt gttgtgccaa atccttttca tcaaagaaaa agctgtcagt
    25441 tacaactggt tttcctttag tgagcgttcc tgttttatca aaggcaatcg cttttaattc
    25501 agaaagcttt tctaaatgga taccgccttt gattaaaatc ccattttttg cagaatttga
    25561 aagagcagct agagaagctg gtgtagcaga tgcgacaagc gcacaaggag aagccacaac
    25621 taataatacc attcctcgat aaaagctttc atcccaactc caacctagga aaaagtgggg
    25681 gagaaggatc attagaggaa cagcaagcaa aatcaatttt acataattat tttcgattcg
    25741 ttcgataaat gaagctgttt gagttggcgt attctgcgct tcttctacta attggataat
    25801 cttagcaaag acagtttcgg aactcgtttt tgttactcga atcgtgatgg gatttccttg
    25861 attcagcgtt cctgcataca tctcatcgcc taaatatttt tctacaggca cagattcacc
    25921 gcttaatgca gactcatcga tcgttgcctg tgtgctttct aaaaagccat ctatcggaac
    25981 agctgctcct ttagcgacaa ataccaagtt atccaccttt agttgagaaa catctacttc
    26041 ttgcatttgt ccattttctt ttaataattg ggctttttgt ggttgaagat tcatcaaagc
    26101 agtaatttct ttttgacttt tattagttgt gtattcctct aatgcaccgc ttaaacaaaa
    26161 gatgaatgtc aacattgctc cttcaaagta attaccgatc aaacaggcac caatagcagc
    26221 taaagccatc aataaatcca cattcagtgt ccgatcattc cacaattctt tcaagccttc
    26281 cgatgtttgt ttatatccac cgatgataat ggcactgata taaaaaagtg gggtgatggt
    26341 aagagagaat cggtcgaaga taaaaccgaa gatcataaat aaaaggcagc cgattgttgc
    26401 tgcaaaagct tttttctgat tagtcatcgt tattagacct ccttgtcaaa acaaggatag
    26461 cataataaaa atgtaattga taataataac agctaaatga gaacgataat ggttatcatt
    26521 tggtgattaa gcttgatgtt gtttttatta tttataatga ttattattaa agaaaataaa
    26581 aaaccagaac atttctcaaa taagagaagt gttctggttt tttctacatt gcaataaacc
    26641 aataaccaat caaaatgata ccaaggataa tacgatacca gccaaaagcg gtaaagtcgt
    26701 ttttcttcaa atagcttagt aagaatttga tagctaagac ggaaaccaca aaagatacga
    26761 atgttcctac aagaagaaca acagtttctt ccattttgaa tgaattccct tcagctaaat
    26821 atttgacgat cttcaagccg cttgcgccaa acatgacagg aatccctaag aaaaatgaaa
    26881 actctgtagc gacaaaacgc gaagcaccaa tcaagataga tcctaggatc gttgcgccag
    26941 aacgggatgt tccgggaaca agtgccaaga cttggaataa cccaacaaga agagctgctt
    27001 ggtacgtaaa gtcattcaga gaagtccaac gaggtgtttt gtttttgttg cgtttttcga
    27061 caataataaa ggcaataccg taaacgatca aaacaatggc aactggtaag aacttgtgga
    27121 aatttgcttc taaccaatca tccaaaggca aaccaatgat gatggctggt atgcaagcaa
    27181 cgatcacttt aaaccaaagt gtccacgtat cttttttttc taccgttgat ttacttggag
    27241 aaaatgggtt taacttgtga aaataaagaa cgacgacagc caagatcgcc cctaattgga
    27301 taacgacatt gaacatcgac ataaattgtg aactggcatc cagtttgatg aattcatctg
    27361 ctaggatcaa atgaccagta ctactgatgg gcagccattc cgttattcct tcaataatac
    27421 cgagaatcac tgctttgata atatttgcaa aaaacataaa ttccttcctt cccaattaag
    27481 atacatctta tagtataccg ttattttttg agaaacccaa tctgtttttt aatattaaga
    27541 aaaacttgag aaataaagca gataagtaca cgaagagaaa aaagaagaca aagccctcgt
    27601 tcagactttg ccttttatga ttctctggta aaaattaaaa tatccccatt atagaaacga
    27661 gtcgcgacta tgtgttttcc cgttatcgat ataagacgta tcattgatgc cgtggataga
    27721 aaatcgaccg tttgcataag tgacctttgt tacgctgcca tttaaaagcc cagcacgaac
    27781 aggctgttta ggatcgatca gattaagcaa aaaggcgatc gtcaaaccat gagatacgac
    27841 taaaacattt ccaccaccat ttttttcgca gtgatgggcg atatcttcaa atcctgtcca
    27901 tacacggtct cgcaatgttt cataagtttc cgcccaccca gcggtatcta agttgtatac
    27961 agcattagca atttgttcaa aagaaacttc tgtggtaaac atttcatcat atgttttaaa
    28021 attcaataca cgagggatca caccccacat ttccatgtca tatccacctt caagagaccc
    28081 gaaacaccat tctctaatcc ttttttcaat aaaataagga atttcttttc ccttagaatg
    28141 ttctcctaga ataatacggg cagtttcgat tgcacgacca ctgtcactag aataagcaaa
    28201 tgaaaagtcg acatttttta acccgcgacc taaataatga atgccttctt gtccttgctt
    28261 tgtgaggggt gtatcacacc agccttgtac tcgttcaatt gtattgaaca tcgtttttcc
    28321 gtgtcgtacg atataaagtg tagtttctgt cattttattc tcctcctaag tcgttttaac
    28381 gattgaaaaa aggatttgtc cttttttcct gttcaatcgt cgttgcatcg ccatgtcctg
    28441 gatagactgc aaattcatca ggtaatacaa acagctgggt ccgaatacta tgaagtaatt
    28501 gttctaaatt ccctgtatac agatcggttc taccgatact tcctctaaac agggcgtcgc
    28561 cagaaacgac aaaatcatca aaaataaagc tgacgcttcc tatagaatgc cctggggtag
    28621 ggacgacgcg aaaagacatg tttcctaaag tataagtttt catttcaaat tcatattctg
    28681 caggcgagac aataacatca gcaatatcat catgtctacc gagtccagaa agattcaaaa
    28741 tcggatcacc tagccatttt tgctctagtg ggctgacata aactgggatt tgataatacg
    28801 cgcgtaaagg ttccactgca ccgatatgat cgtagtgagt atgggtaagc aaaatcgcta
    28861 ccggctgttg gtttgttttt ttgatttctt gtttgattcg ttccgcatct tctccagggt
    28921 caatgatcaa aagtgcttct tcgttgtaga ccaaataaca gttttcttca atcattcctg
    28981 tttttaatct ttcgatatgc atgtgcattc ctccttgata gccaaaactt ctttcatcca
    29041 ttagtataac gcattcattg ctaggaaaca aaaacaggta cttatttctc tttacttatc
    29101 tgaattatca gactatatga aaagtatttg gatattctaa agagaaggaa agtaagtttt
    29161 catagcattt taatatgtcc ttgttacatt agattcatcc aaaaatccaa attctatatc
    29221 ctatatccct gcctaatcag cagggtgttt ttttatgttt ggaaagagag ctctttttgc
    29281 taaactaggt tttttcaggt aatcttagag agaaagaggt ggtggaaatg atcaaacagt
    29341 caaaaatatc tgtgcggcac ttagtagaat ttattttgcg acgtggaagt atcgacaatc
    29401 gtaaaaaaag taaccatact gcattagaag gagcgagaat ccaccgcaag ctccaaaaag
    29461 atgcgggaga aaactatgaa aaagaagtct ttttaaaaac aacagttcaa ttagatgaat
    29521 attacctgat cgttgaaggc cgtgcagacg gtattttcaa aagagacggt atttattata
    29581 tagatgaaat caaaacctcc gaaccacgat ttgaagattt agaaccagaa caagtggaac
    29641 ttttctttca tcaagcaagg gtgtatgcct atatctatag tcaagaacat gatttacaag
    29701 aaataaatct gcaattgact tacttccaga cggcagaaga agtgatcacg agaaaggttg
    29761 aacatcagac aagagagcag ttagaaactt tttttaaaaa gctgactgaa gattataaag
    29821 aatggcttgt ttttcaagaa aactggcgca ctgtacgcaa tgcctctttg atggctttga
    29881 aattccccca tgatgagtat aggaaaggtc agcgtgaact agcggtagct gcttataaaa
    29941 cgatccgtac gaaacagaaa ttgtttgtag aagcacctac cggaacagga aaaacaatct
    30001 ctacgttatt ccccgctttg aaagctgtag gggaagaaga gggagaaaag atcttttatc
    30061 taacggctaa aacaataact agacaagtag ctgaagatgc tatgacagca ttaaaagata
    30121 caggagctga agtaaaaagt gtcactttga cggcgaaaga caagatctgc tttttagatg
    30181 agacgacctg taatccagat cagtgcccat atgccaatgg ttattacaat cggattaatg
    30241 aaggtttatg ggatcttttg aatcatgaga atcagatcac tcgtgaagtc atcgaaactt
    30301 atgcaagaaa gcatacactt tgtcctttcg aactatcatt agatgtcagt atctggtgtg
    30361 acgtaatcat tggggattac aattatttat ttgatccaac tgtttattta agacgttttt
    30421 ttgaagatga aaaaaacgag gactatctgt ttttgattga cgaagcacat aatttggtga
    30481 atcgttcccg agagatgtat tcggcagaac tatcttatga aaaaacgaaa cgaagtaagg
    30541 aagctatacc aaaggaattc aaaaaacttc atcgccgttt caacaaacta ttaaaagaat
    30601 ttgattcgat ccgagaaata gcaaaagaag atcattggga ttatcatcat caaaaagcac
    30661 cggctgaatc tttagtaaag gcaggttatc aattaagcga aaaaattaaa gaatggctgg
    30721 cagaatttcc tgagcaccca tcacaagaac aattgcttcc ttattatttt gatttgcttc
    30781 attttttgaa ggtcagtgaa ttttatgatg atcactacga aacaacagtc gaaaaaacgt
    30841 atcgcgatct gatagtaaaa gagttttgta ttgatccaag cttgtttctt gagcagtcgt
    30901 tggataaagg aaaaagcagt attctttttt ctgctagttt ttcaccactc tcttattatc
    30961 aggaaacact ggggggacaa aaaagtctgg cttacaaact gccgagtcct ttcccagaag
    31021 gaaaccagca agttttcatc gcaaattacc tagagacgac ttatcgaaaa agggaacaca
    31081 gtctggcaaa attagtagag acgattcatg cttttgctga tggaaaggtc gggaattatt
    31141 tcgttttctt tccttcttat caatatttag atcttgcggt ggaagcattc agacagcaat
    31201 atcccgacag caatgtgcta atccaagaaa cggacatgaa tgaggaagaa cgcgaacgat
    31261 ttttaacaaa atttcaaaca aatcccgctg aaacattgat tggattttgc gtacttggcg
    31321 ggattttttc agaaggaatc gatttgagag gggaccgatt gatcggcagt atgatcgtag
    31381 gtgtaggttt gccgcagatg aatcatgagc aggaactgat caaatcatat tatgatgaga
    31441 aggaacacct tggatttgct tacgcttatc agcttcctgg aatgaacaaa gtcttgcaag
    31501 ctggtggtcg agtgattcgt ggcatggcgg atcaaggaat cgttgtattg gcagatcaac
    31561 gattttctag tttttcttat cgacgtttat tcccacaaca ttggcaaaca gcaagagaag
    31621 tacgaagtgt tggggaattg gaagaaggaa tacagcgttt ttggttatca aaagaagcag
    31681 taaatcaaaa aaacgaggaa tgacagacgt cagcctcgtt ttttgattaa cgatagttag
    31741 tgaattgcaa ctcgattggt aagtcaagtt tacgtaataa agcaatcaca gcttgtagat
    31801 catcacggtt cttacctgtc actctaatct gatcttcttg gatttgtgct tttactttca
    31861 gtttttcatt tttgatagct gtagtgattt tcttcgcgtt ttcacgatcg attccgttga
    31921 ccaaatcggc agtttgtcga gctttgccac ctagagcgtg ttctgattcg gaaaaatgga
    31981 tatttttgat tggcacattt cttttgatca gcttaccaaa taatacatct ttgatctgtt
    32041 cgattttgaa atcatcatcg ctcaatacaa ctagattgct gttctctaat ttgatttcta
    32101 ctgtagaatc cttgaagtca aagcgattgg acaattcttt tgttgtttgc tgaatcgcat
    32161 tttttacttc ttctgtattg acttctgata caatgtcgaa gctagcatct tttgccatag
    32221 gaaccctcct gatatacata taaatattta ccatacatct atcttatcat aggattaggg
    32281 cagatt
//
LOCUS       DAKBVG010000008        30641 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000008 DAKBVG010000000
VERSION     DAKBVG010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 30641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30641
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.008"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            147..1016
                     /locus_tag="OUD09_000317"
     CDS             147..1016
                     /locus_tag="OUD09_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HCT9691625.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1225..2250
                     /locus_tag="OUD09_000318"
     CDS             1225..2250
                     /locus_tag="OUD09_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HCT9691626.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2289..3239
                     /gene="manA"
                     /locus_tag="OUD09_000319"
     CDS             2289..3239
                     /gene="manA"
                     /locus_tag="OUD09_000319"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HCT9691627.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            3333..5036
                     /locus_tag="OUD09_000320"
     CDS             3333..5036
                     /locus_tag="OUD09_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCT9691628.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5038..7071
                     /locus_tag="OUD09_000321"
     CDS             5038..7071
                     /locus_tag="OUD09_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCT9691629.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7082..7522
                     /locus_tag="OUD09_000322"
     CDS             7082..7522
                     /locus_tag="OUD09_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCT9691630.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(7722..8684)
                     /locus_tag="OUD09_000323"
     CDS             complement(7722..8684)
                     /locus_tag="OUD09_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT9691631.1"
                     /translation="MIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEAT
                     YHYEKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYE
                     NIFSGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAA
                     MLSLVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLF
                     LSTISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVI
                     IGGDIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8851..9528)
                     /locus_tag="OUD09_000324"
     CDS             complement(8851..9528)
                     /locus_tag="OUD09_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT9691632.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9792..11558
                     /locus_tag="OUD09_000325"
     CDS             9792..11558
                     /locus_tag="OUD09_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HCT9691633.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11573..12010
                     /locus_tag="OUD09_000326"
     CDS             11573..12010
                     /locus_tag="OUD09_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCT9691634.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12023..13174
                     /locus_tag="OUD09_000327"
     CDS             12023..13174
                     /locus_tag="OUD09_000327"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCT9691635.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(13236..13934)
                     /locus_tag="OUD09_000328"
     CDS             complement(13236..13934)
                     /locus_tag="OUD09_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HCT9691636.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(13927..15279)
                     /locus_tag="OUD09_000329"
     CDS             complement(13927..15279)
                     /locus_tag="OUD09_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HCT9691637.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(15502..16902)
                     /locus_tag="OUD09_000330"
     CDS             complement(15502..16902)
                     /locus_tag="OUD09_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HCT9691638.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            17063..17800
                     /locus_tag="OUD09_000331"
     CDS             17063..17800
                     /locus_tag="OUD09_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCT9691639.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            17925..18404
                     /locus_tag="OUD09_000332"
     CDS             17925..18404
                     /locus_tag="OUD09_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HCT9691640.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            18401..19168
                     /locus_tag="OUD09_000333"
     CDS             18401..19168
                     /locus_tag="OUD09_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9691641.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            19398..19967
                     /gene="lepB"
                     /locus_tag="OUD09_000334"
     CDS             19398..19967
                     /gene="lepB"
                     /locus_tag="OUD09_000334"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCT9691642.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(20071..20673)
                     /locus_tag="OUD09_000335"
     CDS             complement(20071..20673)
                     /locus_tag="OUD09_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCT9691643.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            20779..21248
                     /locus_tag="OUD09_000336"
                     /pseudo
     CDS             20779..21248
                     /locus_tag="OUD09_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            21354..21938
                     /locus_tag="OUD09_000337"
     CDS             21354..21938
                     /locus_tag="OUD09_000337"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCT9691644.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            21941..23014
                     /gene="prfA"
                     /locus_tag="OUD09_000338"
     CDS             21941..23014
                     /gene="prfA"
                     /locus_tag="OUD09_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCT9691645.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            23007..23846
                     /gene="prmC"
                     /locus_tag="OUD09_000339"
     CDS             23007..23846
                     /gene="prmC"
                     /locus_tag="OUD09_000339"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCT9691646.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            23839..24018
                     /locus_tag="OUD09_000340"
     CDS             23839..24018
                     /locus_tag="OUD09_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9691647.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            24040..25065
                     /locus_tag="OUD09_000341"
     CDS             24040..25065
                     /locus_tag="OUD09_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCT9691648.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            25240..26484
                     /locus_tag="OUD09_000342"
     CDS             25240..26484
                     /locus_tag="OUD09_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCT9691649.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            26637..27584
                     /locus_tag="OUD09_000343"
     CDS             26637..27584
                     /locus_tag="OUD09_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCT9691650.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            27718..28347
                     /gene="upp"
                     /locus_tag="OUD09_000344"
     CDS             27718..28347
                     /gene="upp"
                     /locus_tag="OUD09_000344"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCT9691651.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(28486..28728)
                     /locus_tag="OUD09_000345"
     CDS             complement(28486..28728)
                     /locus_tag="OUD09_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9691652.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            28849..28921
                     /locus_tag="OUD09_000346"
     tRNA            28849..28921
                     /locus_tag="OUD09_000346"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28882..28884,aa:Thr,seq:cgt)
     gene            complement(29040..29678)
                     /locus_tag="OUD09_000347"
     CDS             complement(29040..29678)
                     /locus_tag="OUD09_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCT9691653.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(29801..30433)
                     /locus_tag="OUD09_000348"
     CDS             complement(29801..30433)
                     /locus_tag="OUD09_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCT9691654.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
BASE COUNT        10433 a         5382 c         6181 g         8645 t
ORIGIN      
        1 tttataagag tttttactca ttaatttaca aaaaacaagc aaaatgcgaa ttttctcaca
       61 ttttttttaa caaaatcaaa gttattgctt gaaagaagaa gaatccatcg ttaaaataaa
      121 gcttgacgta gaggggagca agaaatatga aagattttca attgggggct gccacctatt
      181 taaaaagtgt agccattcgg gtgaaagacc gagataaaat gattgatttt tacaagaacg
      241 ttgttggctt tgatttgaaa agagaagaaa acgaattagc cattttagga acagaagaag
      301 ctggaagtga aatgttgctg ctggaagaaa gtcctagagc gaatgatcac atgggggtga
      361 tcaaaaagct gaaccgtttt tcattgatta tccctagcac agaagaaatg gcagatatcc
      421 tttgtcgtat ccgtaaaatg aattatccaa tcaaagaagc tttagaagat catggacgta
      481 tggggatttt gctcgtagac ccagaagaga atgaattgga aatttaccat gaagagaata
      541 agaacgacca aagagaaatt ccagaaacaa tggatcaaga agcattatta agaaaatctg
      601 aagaaaaatt cagtaaacta tcaacaggtt cttattttga aaaaatccat ttaaatgtta
      661 ctaacttaac aaaagaacgc caatttctta atcatgtact tggtttgaag gtacataatg
      721 aagaagaggg catccatgta ttgaatgaag gagatttcca tgttggtttg acagaagcta
      781 gcggaggcgc aattgatttg cctacagatg aagtgattgg acttgatttt ctgaaattca
      841 tgatcgatac tgaaagcatg gaaggactga ttgctcactt gcagcaacta tcagttgatt
      901 tcttcttaga caaaaagcac acagtactca caatctatga tgctatagga atcgaatggt
      961 ggttcgtcag tgagccaaaa gaaaagaata aaactgacat tcgatgcgac gcttaacttt
     1021 ttctttctta acgattgtcg catgtctcgt cattttatga acggtcatac tatcgcttat
     1081 tctagagata gaaagagaaa atagtatacc gggggaaact gcgcggtttt tcgcgcagtt
     1141 tttttgtgta aaaaaggttg attcctcata gattaagctt taagcttgga ctgtaagaat
     1201 ctgatagaaa agcaggagaa aaagatgtat ccacacacga aacagcttat tttgaaaaag
     1261 atggaagaag gattttatcc aggtgtagca gtcgccttca taaaagaaaa tcaagtagaa
     1321 acatttacag ccggctttgc ccaagtcgtt cctgaaaagc agctgatgac agaagatctg
     1381 ttgtttgatg cagcctcact gacgaaagtc gttgcaacca cttcattagt tttacgtcta
     1441 ttagaagatg gaaagataga actcgatcaa ccgcttcata aatatcttcc tgaatttgaa
     1501 aatcagacaa tcactttgcg tcatttactg acacatactt ctgatatcaa aacatggata
     1561 ccaaacagag ataagctatc tcaggaagaa ttgatcgccg cttatttgaa gctagaacca
     1621 ggtgagcaac taggaaaaga agtaaaatat acagatgccg gtacgattct tcttgggttg
     1681 atgctagagg aaatttacca agaaactgca gtggaaatct ttcaagaaca agtattggaa
     1741 ccattaggaa tgatgaacag cttatttctt ccttttcctt ccaaaaaaat cgttcccaca
     1801 gaacaactgc cttcgggtga ggttttgcgt ggacaaactc atgatccgaa agcaagagtt
     1861 ttggctgaac atgcaggtaa cgcgggtctc tttattgatt tgaaagacag cattactttt
     1921 gtccaaatgt atttgaacaa gggaaaaatc cgtaatggtc gttttttaga agaagagacg
     1981 attgattctt tactgcaaga tcaaacaccg aaaaaaaatg gctatcgttc tttaggatgg
     2041 gacttaaaaa aggatgtaga agacggtcat gcgattcttt ttcatactgg ttatacaggt
     2101 acgtttttct tgattgatat aaaaacacaa gaagcgttga tatttttgtc aaatcgcgta
     2161 catcctgtcg atcatcgtgc ggagtatctc atatgtcgag atgaaattgt tgaaaacttt
     2221 ttgaaagaga aagcggtatt accgaaataa tgctataata gctatgaaaa atgagaggag
     2281 tttgaattat gcaggaacct atgtttttaa aaccagtttt tcaagaaaaa atttggggag
     2341 gaagtcgttt acgttcagtc tttggttttg atatccctaa cgataaaatc ggagaagatt
     2401 gggcgatcag tgcgcatcct catggtgtca gtgtagtaga aaatggcgaa ttcaaaggga
     2461 aaagattaga tgaactatgg tcagaacaca aggaattgtt tggccatcct tcagaaccag
     2521 tgtttccatt attgatcaag atcttagacg cagaagatga tctatctgta caagtacatc
     2581 cagatgatgc ctatgggatg aaacatgaag gtgaattagg aaaaactgaa tgctggtata
     2641 tcatcgatgc tgaacctggt gcagaaatca tctatggtca tcatgcaaag acaagagaag
     2701 aactagctga gatgatcaaa gatgggcgct gggatgacct tttgaagaaa gtaccagtta
     2761 aaaaaggtga tttcttttat gtaccaagcg gaacgatcca tgctattggt aaaggaatca
     2821 tgattttaga aacacagcaa agttctgaca cgacttaccg cgtgtatgac tacgacagaa
     2881 aagatgcgca aggacaaaca agagagctgc atatccaaca atcgatcgat gtaacaactg
     2941 tccctgcgaa aacaccagaa ttacaaatca aagaagtccg aaaagggaat tcatcgatcg
     3001 tcacttattt agaaactgag tttttcaatg tatatgagtg ggatattaaa ggaatcacat
     3061 ctttcaaaaa acaagcccct tatacattga tgacggtaat cgaaggagca ggagaccttg
     3121 tcgttgacgg aaaaacatat ccattagaaa aaggaacaag ctgtattatt ccaaatggcg
     3181 tttctgagtg ggcggtccaa ggtgaactag caatcattgc gtctgaacca ggcaaatagt
     3241 tcccagatag ctgaaagcag cagacggttt ttcgtttgct gctttttcta cataaaatcc
     3301 gtccatcaat aaatagaagt ctggtgaaaa agatggaata caaatggaaa gcaaacaaca
     3361 gcagtcgcgt gtctatgaaa caactgagaa aagctggaca tcacttaaat gaaatgatca
     3421 tgccaaatct aagtctgttt atcgcttggg gaatcttgtc attagcaaca aaaggtgtgt
     3481 ctggcgattt gcacaccaca ttagaagagg tatgccattg gatgatccag ctgcttttac
     3541 ccatcttgat cagttattca ggcgggaaat cggtaggggg acaaaaaggg gcagtcgccg
     3601 gtgcaattgc ttctttaggg ttgatcgctc gttccaatgc tccacaaatc gtaggagcga
     3661 tgattatcgg gccgcttgcc ggcatcgtat atgaacaatt tgtttctaga ttccatgtga
     3721 aattcaaggc tggatatgaa atgcttgtca gcaacctact agtaggactg actggtagcg
     3781 ttatttgtat aattggaatc gtttttattg aaccagtttt aaatagtgtt cacttgttgc
     3841 tcgcctccgt tgtgtcttgg ctagtcacaa tgaatctgtt accacttgtc agtctgatct
     3901 tagaacctct aaaagtacta tttttcaata atgcaatcaa tcatggtgta ttgacacctt
     3961 taggaatcga attctcacaa acagtcgggt actcttcctt atttttgatg gaggcaaatc
     4021 ctggacctgg tttagggatc ttactttcta tcctttgttt tgcagaaaaa caggaaaaag
     4081 taaatgcgag tggagcattg atgatccatg cgattggcgg gattcacgaa atctattttc
     4141 catttgtctt gctgcgaccg tatctatttt tagctgtaat ggctggtggg gcaagcggca
     4201 cgttgatatt tcaatggtta gatacgggat tagctgcacc tgtatcgcct gggtcactac
     4261 tcgtgatttt tgccaatgcg ccttatccac aactagctgg ggtaagtata gggatagcaa
     4321 tcagtacatt tgtgaccttc atttgtgcag cgatactatt gaaattacca aataaagaaa
     4381 aagagaacaa aacggtcaaa gaaaggggag aaatcttgga accatctatc attcattccg
     4441 tcatttttgc ttgcgactca ggtatgggat caagtgctat gggagccgcc ctgctgaaaa
     4501 agaaattagc agcagaaaaa atcgatcttt ctgtcggata cagctctatc tatgaattga
     4561 ataaccaagt aaatcagctg gttatcgtgc aacatgaact agcgggtctt gcaagagaaa
     4621 aagtacctca aagtcaaatc tatgtactgg atcacttttt ggaaacagat atcttgctgt
     4681 cacgaataaa agaagcgatc caatttccaa agcgggaaga aaaaaaagag atagcagata
     4741 tcacagaaca aaaaacgtcg cctgctaata ttgtattttt gtataaaaac aacagacgcg
     4801 gctcacaaac tatgggcatg acgattttaa gtcaattagc aaaaaaagcg aaaaaacagt
     4861 ttgttatctc aaaagaatca atagaaaaaa tcaaaatcca agaagaaacg atttatatcg
     4921 cacaaagaga actagctgta actgaacagc tagaagaaaa gatccctcgg ctagttctag
     4981 ttgaacattg ggtagctacg aatgaatacg aaaaactcgt gaagggagaa atataagatg
     5041 tatttatcgc ccagagaaaa acaattatta gcagaatttc tcagtagccc gtcacctgta
     5101 tctatccaaa aaatgatgaa tttattgaag gtcagtaaac ggactattta ccgcgaatta
     5161 gataatcttg aagtctcatt gaaatcggta aatgcttctt tgaaaaaagc agctagaggc
     5221 tcatttgtca ttgaggcatc agaagaagta ttagagcaat taaaagaaaa tacagatggg
     5281 aaaagtaatg acttatccac tgctaaacga caacacgcga ttttagccga gttgttactg
     5341 acgacagagc cgttgagtct tgctcatttc atggaatcat atcgcatcag taataccact
     5401 ttttatgcag atatcaaaca gttggaagaa agtattcgcc aactgccttt ggaaatcatt
     5461 cggaaccagg gatacgagat cgaaggaccg gaaaaatacc gccggcttct gacagcgaat
     5521 gtattagaat tagaaatcaa tgaatatgaa ctttttcata gtatttcttt cgatgcatca
     5581 ctgaattatt tttttcgatt cgttgatccg caacatctat ctcttgcacg caaagtaatc
     5641 ggggaagaat tgatccaaaa aaagaccaat ctaagtgata gaaagcttga acatttggtt
     5701 ttgatgctga cattgacgat ggagcgtgtg acgaaaaatc atttactgac aaatgagaca
     5761 tataatagtc tggcaaacaa agaattacta cagacagcta aacgattgtt ttcacggatc
     5821 gcttctgaga caaaacaact ttatccagta aatgagatcg tcttttttgc aggattatta
     5881 aatgacttca cgaactcatt tgatcagaat ttctttgaag agacgttcga tacacagctt
     5941 gcttatttag taaaacaact gattcaagaa gtaagtgaag agacgaatgt ccatttttat
     6001 gaagatgaaa ctttgtacaa aatgctgttg actcatttat ctggtgtgtt ttcaagagcc
     6061 gttttacaag aagagacact gaccaatccg attttagaaa aaatcatggt tcaatacgag
     6121 gaagttgcga atgcaatccg taaagcagta gagcggattt ttcaagaaaa aaaattatcc
     6181 gaagaagaga tcgcttatat ggtccttcat tttgcgaatt cattggaacg aagtccgaaa
     6241 gaaagcaata tcagtattgc aggattttcg ccaagtggat tagcttctac tagcatgttg
     6301 gaactacgat tgaaaaggta ttttcccttc atccaacaaa ttcatttcta tcggattgct
     6361 gatttaaaaa aaatcgattt acaagaagaa tatgatgttg ttatttctac atctatttta
     6421 ccaggatacg gtggaaaata tattttagtc tccccgctac tgctagaaga tgaagtcaag
     6481 cagctaaaag aagctttttc gcatattgag aagaaaaaaa ggcaaaaaat tacgtataac
     6541 agcaataacg agtggatgat agaagatact tatgaggaaa caatgcattt catggatcaa
     6601 atcaatcaat tactcggtcg tttttttatc caagaactgg ataatcctga gacgatttcc
     6661 gaaacgattc gcagcttatt tccttatttt cctgaaggac tagtaacgga tgaagaggtg
     6721 gttcatcgcc ggctgcttaa gcgatatgaa caagcaccta tcggtattcc tcatacaaat
     6781 atggggttat tccatacatc cagctcattg atttctaaac cgatcttttg tattttcaat
     6841 ttgaaacatc cgttgaccat tgaagggatg gatagacagc cagtcgattt gtcccgtatg
     6901 ttagttatgc tggcgccttc gccaatccaa gaaaaagacg ggattctttt aggaaagatc
     6961 agtggagcaa tcatcatgaa tgacttgaac accgagatct tccgctcagg aaataaagaa
     7021 atcgtttatc agttgttagc gaaaatcctg attgaagaaa taaagaaatg aaggtgaaaa
     7081 gatgatacag ctaccagcag aaaaaatatt gctcgatcaa tcctatcaaa attggaaaga
     7141 agcgttgacc gagatcggcc agtggatgac ccaacaaggg ggaatcaaac cggcatatat
     7201 ccaatcaatg atcgaaagac aagaaaaagc gagtgtctat atcgggaatt tcgtagcctt
     7261 gccacatggg actgacaaag atgaagaagt agtagaagaa ggaatctatt tagtccaagt
     7321 gcctgatggt gtggactttg gaacaaaaga ggaaccaaaa atcgcgacac tcttatttgt
     7381 agtggcttta aaaaaagaac atcagttagc ggtgctccaa gatttagcct ttttctgttc
     7441 agacatcgac caagtaatgg cgctcagtga tgcggaaacc attgaagaag tccaaatgat
     7501 ttttgaacat ggacatatat aaccaatgtt cacagtaaaa aaagctgcct tgcatcaagt
     7561 aataacttca accaagataa aaataagagg agctattttt gttgggaggt tcttattact
     7621 gcggatgcaa gcttttgaaa actatttatt ctagaaaacc gtttatttga aataggggac
     7681 ggtgacaagg tttttgtcac cgcccctatt atttttgtgt tttaatagct cttttttata
     7741 tagatagtgc ccatacctat gaataataaa acgatactcc atataccaag tgtcaagata
     7801 ccattcatga aagtaacctg ttctgaagct agtggcgaga aaatatctcc tcctatgatc
     7861 acattttgaa aatcaacata agaagttggc aagtaatgac tgattgattc tatccatcca
     7921 atcgaagaaa acaagcgata ctctgctaag aaaagacaaa gaagtgtacc taaaagattg
     7981 actagagaat gttcaaataa agcagataga agaaaactga ttgtacttaa aaagagcacc
     8041 cacaacaaca ggaacagaaa gctctggaaa agatactcat acatcggcag gattcgtatc
     8101 tcctgaccaa tgatcgttcc tacaggataa cggaagtctc ccaaaccatt tttgattatt
     8161 actagtatag agacgatcaa aaatggcatc acgagactta gcatagctgc accataataa
     8221 atggtaaaac ggatgctatg tttctttacc atactatccg gtaatagatt gactagagag
     8281 attacctgct ttcttttttc ataagagatg atggaagaaa gaaacaaggc ggttatacat
     8341 aaaatcaaaa aactagaaat cattcctgaa aaaatatttt cgtagtaatt tgctaaagag
     8401 agatgcgctg gcaagtcaaa aatagaatat ctttggatct gatgttcttt catataacgg
     8461 agcaactcga cacgtttttg ttgttcaatg attggaattc cttgtctttg accagaaatc
     8521 agtcgatcca gtatatcttt ttcataatga taagttgctt cgaccatttg ctggacattc
     8581 cctgtgtttt ttgcatgtaa gattgcttcg atcagtttgt tgcttttctc taaactttcc
     8641 acaaattctt tctcattctc atcttctttc aacgtttcaa tcaaacgttg catctcttca
     8701 tagttatgat tcagttcatc gaattctgta taagcaagtt tatgatcaaa aaaagtggga
     8761 atgcaaagag cacgaccaac aatagaggaa ataaaaagtt tactttgttt tttagtaaga
     8821 gattgcgttc aaatgtaagt aaagacatag ttacacctca tataatttac gataaatagc
     8881 taaaatctct tccatatttg aggattctgt gacgatttct ccatttttta gaaaacatgc
     8941 tttagaacaa atcgattgga tattcgcgat agcgtgggaa gaaagaagaa tcgtcttccc
     9001 ctctttctgc cattgtctga tgaggctatt caagatatca atacttcctg ggtctaatcc
     9061 gttcaacggt tcatcgaata acatgacaga tgaatcactg atcaagtaag cagcaagcaa
     9121 gacatgttgt ttcattccta gagacatttt tccaatcggc aaagtaaggt actcagtcat
     9181 cttcaatcgt tggaaaacag tttggcattc tactttgctc tgccaatagt atttgatcaa
     9241 agtcaaataa tcaacagccg atagatagtc ggataactga ttcgttgatt ctagaaaaaa
     9301 gaaagaccgg cgataatctg tataagcagt aggagagatg ttgtgaaggg acaattgatc
     9361 ataagttgtt ggcagcagtt gggcaatgcc attcagcaaa gttgttttgc caaatccgtt
     9421 cggggcaatc aaagcagcta cttctcctga ttctactaaa agattcaatt gtcgaatcac
     9481 acgtttttta ccacgtttca tgttcatgtt tttgatcagt aatgtcatag ataggcctcc
     9541 aatataaaat ttaataacat aatagaatgg aaaacatcta aattaaatgc taacaaaagt
     9601 tattgtttgc atattataaa aaaattaaaa taacagtttt atttaaaatt gaagttttct
     9661 gataccgaaa tctttttatt ttgctgacat actcaaacct ttggcacaaa gtttcgtgcc
     9721 aaagtacgac tatttctctt tttagtaagc ggatacaatt agagtatcaa aaggagagag
     9781 ggagagacaa aatggaacgg acgattccca aaaacaacat ttcgctaaaa gcgagggtac
     9841 aaaaattagg cagtacttta tcaagcatgg tcatgccaaa tattggagca ctgattgcct
     9901 ggggtgtatt aacggctcta tttatcccag atggttattt accaaatgag gctttcgcta
     9961 ccatggtaag cccaatgcta acttatctga ttcctttatt gatcggttat actggcggaa
    10021 aagtaattgc tggtgaccgc ggatcagttg ttggggcaat tgcaacgatg ggtgtgattg
    10081 tcggaacaga tattccaatg atgctaggcg cgatgatcat gggacctctt ggtggatatg
    10141 ccatcaagaa atttgatcag ctattccaaa aacggatcaa atccggtttt gaaatgctag
    10201 tcaataattt ttcagcaggt cttatcggtt ttggattagc gttattaggt ttttctgcga
    10261 ttggtccagt tgtcgatgca ttgactcaag caatggcaaa aggtgtagag atcattttat
    10321 ctgcccattt gattccattg acaagtattt ttattgaacc agcaaaaatc ttatttttga
    10381 ataatgcaat caatcatggg attttgacac cattgggaac tgaacaagta gcagaggctg
    10441 gaaaatctat tctattcttg cttgaagcaa atcctggtcc aggacttggc gtattacttg
    10501 cttttacttt gtttggcaaa ggagcagcaa aatcttcagc accaggagcc atgatcattc
    10561 actttttagg cgggatccat gaaatctatt ttccttacgt tatgatgaaa ccgttattat
    10621 tcttatcagt tataggcgga ggaatgtcag gaagtttcat tttccaattg atggatgctg
    10681 gactaagagc gccagcttct ccagggtcga tcattgcgat tctagcgatg acaccgttca
    10741 attcttacct tcctgttatt ttgggcgtga ttgttgccgc agctgtttcg tttggtattt
    10801 ctgcagtaat tttgaaagcg gatgcgaaag atacaaatga agacttcgaa aaatctgttc
    10861 aagagacaaa ggcagctaag caagcttcca aaggtgccca aggtattcct caaggagcta
    10921 cattctctgg cgtacaaaaa atcatttttg cttgtgatgc aggaatggga tcaagtgcga
    10981 tgggcgcttc gatcttgaga aaaaaagtac aggaagcaga cttgcctcaa acggtgacca
    11041 atcaagcaat cagccaactg acagatgact cgaatacatt gatcgttact caggtcgaac
    11101 tacaagaaag agcaaaacaa aaagcaccga gcgcgcgttt tgtctcagta gaaaactttt
    11161 tgaattcacc tcgatatgat gaaatcattc aagaattaag cagcaagcag aaaacatcag
    11221 tttctgaaat tgagaatgag ccagcagaag agtttctttc agcaaacatc aaacaaatca
    11281 aagagattct acttgtccac gatgatcgtt ccggatcagc aacaatgggg atgaccgtgc
    11341 tcaaagatat attagacaag aaccatttaa ctatcccgtt aagaaaagtc aatatccaag
    11401 atttaaaaga tgaacctgat tcactgatcg tagccaaaga aagtttagcg gaaaaagtac
    11461 agatggctgc acctcaatcc attcatttat cagtcaacag catggtcaat ccgcaaaaat
    11521 acgaatcgat tgcacaatat ttgaaacaga cagcttaagg gaggaaaaca gcatgcaaag
    11581 tttgccattc gacatgatcg agctcaatcg atcttttaaa accaaagaag aagcgatccg
    11641 ctattgcggt cagatgcttg tagaagcagg gtgcgttgat gaggaatata tcgatgccat
    11701 ggtagataga gacaaaatgt tatctgttta tatggggaat tttatcgcga ttcctcatgg
    11761 aacagatgaa gcaaaaaaat atgtgaaaaa atccggcatc tgtgtcgttc aagtaccaga
    11821 tggtgtagat tttggtaccg aagaagaaga aaagattgcg actgttttgt tcggtattgc
    11881 aggagtcgga gatgagcatt tgcaacttgt acagcaaatc gcactttatt gcagtgatat
    11941 ggacaatgtt gtccagctag cagacgcgct cagcaaagaa gaagtaacgg aaaatctagc
    12001 aattgcttaa ggagagagaa aaatgagagc agtacatttt ggtgcaggaa atatcggtcg
    12061 aggatttatt ggagaggttt tggcagctaa tggatttgag attactttcg tagatgtgaa
    12121 tcacacgatc attgatgctt taaatgaaag aaaagaatac gaaatcgaat tagcagatga
    12181 atcaaaagaa aaaatcactg taaaaaatgt caaaggaatc aacaatcaaa caaatccaga
    12241 aaaagtaatc caagaaatcg ctgaagcaga tttagttact accgccattg gcccgaatat
    12301 cttacctttt attgctgagt tgattgcaaa aggtatccaa gaaagagaaa aggaaggaag
    12361 cacaacatct ttagatatca ttgcctgtga aaatatgatt ggcggttcag catttctaga
    12421 aaaagaagtg tataaatatc ttccggaaac atcatttact gacaaatata tcggctttcc
    12481 aaatgcagca gtcgatcgaa tcgtcccatt acaacaccat gaagatcctt tattcgtcca
    12541 agtggaacct ttcaaagaat gggtcattga cgatagccaa agaaaaaata aaaaaatcca
    12601 attaaaaggg gttttatatg tagatgatct agaaccatat atcgaacgca aactatttag
    12661 tgtcaatact ggtcatgcga ctgtcgctta tacaggtgcc ttgttaggtt accagaccat
    12721 tgatgaagca atgcaagatg cgttagttgt ggcacaatta aaatcagttt tgcaggaaac
    12781 aggaagtttg ctgattgcca aatgggggtt tgatgcagag cagcatcatg cgtatattga
    12841 aaaaatcatt catcgcttcc aaaacaaaaa tatctctgat gcgatcacac gtgtcgctcg
    12901 gacaccatta cgtaagttag gttatcaaga aagatttaca cgtccagtga gagaactaca
    12961 agaacataac ttgacttgcc cacatttgac ggcaacaatg gggattattt ttaattacta
    13021 tgatcctgaa gatgaacaaa gccgtcaatt gcatgaaatg aagatccatg aaaacttgga
    13081 acaattgatt caagaagtca caggaatcaa tgatccgaaa acaatcggga atatcaagca
    13141 aaacgtgaac cgttatgcca agcaagtcgc ttaaaatagg atacgaaaaa ggggaatgtg
    13201 acgaaatttt tgtcacattc ccctttttcg aataactagg ctgtttgtcc ttcaagatag
    13261 ggcagatcca attctttacc gtaacgttgt tctaaagcta ggcggatcaa acgagctgcc
    13321 aggttctcat ttcttgcaag gataggtcca tggaaataag aaccgaagac atttcggtaa
    13381 atgacacctt cagattggtc ttcaccatta tttccttgac cttgtaccac tttccccaaa
    13441 ggtcgttcac cttcacctaa gaaagtccga ccattgtggt tttcaaatcc ataataagtt
    13501 tcattgaatt cttcgttatg gataacgata tcaccgataa accggttatt ttcctgactt
    13561 aacgtgtagt gatccagtgc actgattcct tggatctttt ctccattagc tcccatgtag
    13621 tagtgaccta gcaattggta gccaccacaa atggctaaca tgacgccatc attttcgata
    13681 tagttgatca aagaatcttt ctttttctgg atgtcttttg aaatgattaa ttgctcaaag
    13741 tcttgaccac cgccaaagaa aaccaagtca tatttatcag catcaaaagg ttcatgtata
    13801 ctaacgatct ccgtatggca agaaaccccc attttttctg ctatatattt caacatcaaa
    13861 aggtttccgt tatcaccata tgtgttcatc aaatttccat aaaggtgggc gatattgagt
    13921 tcataattag ccacggttca ttcctccttt gataaatcct tgagcagcta gttctttacg
    13981 aagctgcaat acagcagtgt aagtagctaa aatatagaca tgctcagttg gcaattcttt
    14041 gattttttcg attacttctg gtaattcttt tgtttctgtc aatttatttt ctggaatccc
    14101 ggcaactttt agacgtaaag tcatatctgt atgacggtct cctccagaaa taacagctgg
    14161 gatatccata tcggcaaagt tttcaaaatt accatcccaa atccagctga catcgattcc
    14221 gtctgcgtaa ttggcattta ataaagccac taaagaaaat ggataaggag ctagaccaat
    14281 catatcgatt acttgattga tacctacagg atttttgaca agtactaatg tacatttttt
    14341 cccattgata ttgatgactt cttgtcggcc gaatactttc tcgtcatatc ctaaaccttg
    14401 tttgatttta tcaggatcga cattgaagta ctctgccact gcagtagctg ctaaagcatt
    14461 gtagacatta tacataccgc caacttcgat tccgtattca tgaccatcga tcacgaaatc
    14521 tgctgacaca ttgtccatcc gtaccatatc agttaatcga taatccaatt caggacgttt
    14581 gaagtcacaa tttggacagt aatatttccc taagtttgca taagtgatca ttttataatg
    14641 caagatgtga tgacaatttg gacataagac accgtctgta ttgtaatgag ccatttgttc
    14701 acgatcttct tcatggtcaa agccataata ttttctagga ttgactgttt cgatagaatt
    14761 gaagattggt gaatcaccgt tactgataat cgttgcattt ggtgactttg cggcaccgtc
    14821 tacaatcatt ttataggtcg tatagatttc tccataccga tccatttgat cacggaaaat
    14881 atttgtaaat aagaacagtt ctggtttcat atattctgtg actttgctca aacttgcttc
    14941 atctatttct aggacagcaa actttttctg tccttttttt ggttttgcct gcaagaaagt
    15001 cgatacaatt ccctgtacca tattcgcacc agtagggttg gttagaactt catcgaattg
    15061 ctggcgcaaa atattgactg ttaaagcggt agtcaatgtt ttaccgtttg ttccggtgac
    15121 aacgacgact tggtagtctt ttgccaaact atctagtata tgtggatcga ctttcagcgc
    15181 tagtttacct ggtaaacttg atccgccttt aaaaaaagtt tttaatgccc attgcgacgt
    15241 ttttccaact gcaatcgcaa aatgactgcg tattcccatt atgtaacctc caaaaacgat
    15301 ttgctgacga ataaattcaa ctaatcatac catatgttga aaaaaaggtt aaattgcttt
    15361 tcttattctt aagaaaaggt tacggctgaa agtgcatgaa atcggacaaa atcgcatgat
    15421 ttaacaaaaa aacagcttga aacaaaatga ttgtttcaag ctgctgtgat tttattattc
    15481 cctcttacct atattcccat tttaaccgat aataattttc tcgcttggat attcatagcc
    15541 tagatctcgt gtttcttttg gcacgaataa catcaatgta tatagcattc cgatccgccc
    15601 aataaacatc agtgtagcaa tcgtgatttt gcccacaaca gacaagtctc cggtaatccc
    15661 taaagataaa ccagttgttc caaacgcaga agttacttcg atgatgatcg agatcagcgt
    15721 ttgttcttct gtagcagata aaaaaacgat acaaaagaaa cacataccaa gagaaagcat
    15781 aaagacaacg actgattttc gcacatcatc ttgatcgatt ctccgtccaa aaatattgat
    15841 gttatcttca cttttgagga aggaatatag atacaaaccg atgattgcta cagtagttgt
    15901 tcgaatcccg ccgcctacag aactaggact gcatccgata aacatcagta aagaaaaaat
    15961 gatcagtgtt gtgatttgaa aatcacccaa atcgtgaatc tgcagaccag cattacgagt
    16021 cgtaatcgaa taaaacatcg aattgatcca tttgacactc atattggaat cttggaataa
    16081 atggtccttt tccaatagat aaatcaagac agttcccgaa acaaacaaga taacaaaagc
    16141 taacacagct aatttggtaa acaaggaaaa acgaaacgga agcttcgcat tggaacgttt
    16201 gtataatagc cattcccggc actccatcaa tacaggaaaa ccaattcctc cgacaaaaat
    16261 caaaaacatg atcaggatca agaaaaaata gtcatgtgca aagggcttga tggaatcccc
    16321 agtgacgtca aacccagagt tcgtgacagc cgatatcgcc tgataaaaac cgtaaaaaac
    16381 ggcatcacgc caacgatcaa aatagcctct gtaataaaaa taaatagaaa agaatgtccc
    16441 aaaaagtaat tgaaaccaga ttaaaatagc aaaagtgatc cgaatcagtc ggacgatacc
    16501 agacaaacgc ggttgattca tatccgtcat gatcagctgt cgctgtttca gtgtgatccg
    16561 tcgtttagaa aaaatgacaa aagcagtaga aatcatcatg atccccaaac caccaacttg
    16621 aaataatatt tccagcagta taataccgtt atcattgaac acagaattga tatcaaaagt
    16681 cgatagtcca gtcacactca ctgtactgat tgccatgaac agcatgtcca caaaagaaac
    16741 atggctatct ggttcgcgaa aaatcggcat ataaaataga acgagtgata agaccgtcat
    16801 caatatataa taaaaaacga tgatttggat cgatgagaaa tgattggcag caaacttctg
    16861 tcctcgccgc tgcagccttc gaaagaataa atcaacatgc acagtactat cctcctgaca
    16921 atagtcgttt ctaccagtat acaaggggaa ttaggaatac tcaaagaaat tatcaaacag
    16981 ataacaatac agctttcgat taaaatagaa atggcttata ataaggaaaa tcctagaaaa
    17041 aagaaggagg aggaataaca aattgattaa tgctcaacaa atcgtagaaa aaatgaaaaa
    17101 tcaaaaaata aattatgata cagttctgaa aaaaatgatc atgcaatggg agaaagaaga
    17161 agtaaagcct aagatattaa tacatacatg ctgtgctcct tgcagtacgc acacattgga
    17221 attcatgtct caatatgcag agatcacgtt atttttttca aattcgaata ttcatcctaa
    17281 aagtgagtat ttgcgaagat tacatgaaca aaaacgattt gtagaccaat ttaatgagaa
    17341 aactgaccat catgttgcgc taatcgttga tgaataccgt ccaaatgaat tcaccaaaat
    17401 ggttttaaca aaccatcttg aaaacgaaaa agaaggtggt gctcgctgca gtgcatgctt
    17461 caatatgcga ttagatcttg ttgcgcaaaa agcgcaagaa ctaggatacg attactttgg
    17521 aagtgccctg accatctcgc caaagaaaaa tgcagctttg atcaatcaga tcggaatgga
    17581 tatccaaaag atttacggga cacaatactt gccaagtgat ttcaagaaaa acaaaggcta
    17641 tgaacgctct ttgcaaatgt gcaaagagta tgatatttat cgccaatgtt attgtggttg
    17701 cgtgttcgct gccaagcagc aaggatgcga tctgaaagaa atcaatcggg aagcaaaagc
    17761 ttacttaaaa acatcaaccg tagtttttga ggaaaattaa agaaatcttt cggaaaaacg
    17821 taagaaaaaa ataaagaatt tatggtagaa ataaggtaga ctgtataatt tgtccgtagt
    17881 aaagtaagga aacaagaata aatttcaatg aaggaagaga taagatgcga aaagtagcac
    17941 ctattttgaa gccaatcgct gtattaacaa tctcgacgat tgtcgcgact actacaggta
    18001 ttgtgaatag ttccagctac cgtgcaccaa gttcaagttc tagtactgta gaagaaacaa
    18061 caccaactac ttcagtctcg acatctgaac caacaacaac ggaaagtttg aatgaaacca
    18121 ctacgacaag tacatgggac gatacaacaa ctagttcgtc gtatgatgag acatatacaa
    18181 ctgacagctc aagcgaaaca agtaatacgg taccagatgg cactccatcg gattcaacca
    18241 atccaagtac aagtccggat acaagtgcta gctccgaaac gataccaagc agtacgactg
    18301 tagatgataa cacgcaaaat gatgatgcag cacgtacagc acagcaaaac caagattcag
    18361 gaaataccca aacgtataac cagcaagcag gtgattctca atgacatttt tactagacct
    18421 tatcaacggg gtgcataaat tccttggcta tttagacatc agtcctaaat atcttaaccg
    18481 tggatatacg attttaagtg tgattcccac gctttattta ttgagaattg tttatggact
    18541 ttggcaaaac caaaactatc ttcaattttt cttatatgga cttgcatttc tagtgcttgt
    18601 ttattttact gttttaaatg tattttatta ctttttagat aaaaacacga aagccgatgt
    18661 cactcagctt tttgtgaagt atttacctga tgaagcgttc aacatccaga cagaagaatc
    18721 gatagataat ggaaattcga tcgataaagt gaatacacaa gaggtcatgg tttcttatga
    18781 cgaagattat caattgaaat tagccgagaa tatgcggtat ttgattggaa atggtgagat
    18841 caaaacaaat gaactcggtc gcatggatgg atttctggtc gatagaaata cattgtatcc
    18901 ttattactat gtcaagaaaa caggagagaa agagtatgtt ctaaatattg gacgaagcta
    18961 ctcggacatg gaaaaagtag ggaccattcg acttgactca acagaagaca cattggaacc
    19021 tgtcggattg ttcatcgtcg gcggtgattt tgtcaaagac gggttacgct atcatgagcc
    19081 ttatcgtctg aaattgattg caaaaaaggc acagtcgcag gaagaaccag tggtttatag
    19141 tcgtagccaa agaagaagac attcataatg tgatgaaacg ccgtgtatca gtggttgcga
    19201 taaaaacgtt tattcggaat aagggggatg tgacacgtct tttgtcacgt tcctttttta
    19261 tataacgaga aagataagac gaacacatca cttattcttt cctttcattt ataaacattg
    19321 tagaataaag aaagtacaac gaagtattgt gaaaaaacgt aataaataac gaggaaaata
    19381 caagtaggag gtaagctgtg aaagaatttt tgaaaaattg ggggattttg atccttgtat
    19441 tagcggctat tttattagct agagtgtacg tgtttacacc agttactgta aacgggcatt
    19501 cgatggaccc tacattaagt gatggacaac gattgatttc atctaagatc tcaaactatg
    19561 aacgaatgga tattattact acaaaggaac cgggggatga agaacggatg attgttaaac
    19621 ggatcatcgg tatgcctgga gatacagtca agatggaaaa tgaccagttg accattaatg
    19681 gcaaaaagta tgatgaacct tatctggatg agttcaaaaa agaattttca gaagataagt
    19741 tacaaggaga atatgcttat agctcaggat tccaagcaca agcagaaagt tcatccacct
    19801 ttacgagtga ctttgaatat actgtgccaa aaggaaagta tcttgttcta ggcgataacc
    19861 gcttgatttc aaaagacagc cgaatgtttg ggttagtcga caaagatatg atccaaggaa
    19921 aagttgtttt ccgttattgg cctctttcag aaattaaaat catgtgatga aaaaataaaa
    19981 agaagctgtg acatgttttt tgtcacagct tctttttccg aaaattcctt cttatttctt
    20041 ggagttgctc actttttttg caatctcatt ttaagctttt tggctgatgg ataagaaaag
    20101 cgaagcgttt ttgttcataa atagcttttc cccacgatgg aaggtatgac tagtagcaga
    20161 gacaaagccc gttttttcca atcgattatt caattcttct tggctaaagc cattatggac
    20221 tttcggatga ctaatttgtt cattcttgtc aaagtctacg atgatcaatt gaccgccagg
    20281 agctaaaatt tcataaagtt ttgtaagaat attctccgta tcaggaatgt gtaaaagtac
    20341 aagtgataga aggatgagat tagcttggac agcaggaaat tcgacagaag catcagcgtg
    20401 gatcgttcgg acatttttca gatctgctgc ttggatcttt tcttctgcca ttttcagcat
    20461 agtttctgaa gcatcggcaa tgatcagttc tttgaatctt tccgcaagcg gtaaactaac
    20521 aagcccagtt ccgcctccgt aatccagcag gactttttgg tggctgtcgc gcggtagata
    20581 agtagttagt tcttcgcgaa taatcttggc taatgcttga cggtcagggg aatcataatg
    20641 gttagcgatg gaatcaaaaa tattttttgt catcaaatag ctccttacgt aaaactttga
    20701 aattttaact tattatagca cgtgaaaaga aagaaagcat gctatactca attcaaaaag
    20761 ggagaatcta gaaagaatat gccgatatcg tttgtaaaag atagagagga aaaaggaaag
    20821 tgtgtccgag aaatattact agacttgcct gagtggtttg ggcttcctga atcaacggaa
    20881 aaatatatcg aggaatcaag taaactacct ttatggtgtg aaaaaagaaa agaagaatat
    20941 ttaggcttta tcactctttc acagacaagt gaagatactg ccgaaatcta ttctattgta
    21001 tgggaataaa aaaagactat catcaccaag gattaggaac aaaacttttt aaagaagcag
    21061 aaggttacgc agcaaaacat tacaaatatt tgcaagtgaa gactgtagac gaggggcact
    21121 actctatata tgatcaaact atttgtttct acgaatcatt aggattttca aggttggaag
    21181 tttttccaaa tttatgggat gaatggaacc catgtttagt tcttgtcaaa aaattggaac
    21241 aaaagtgagc aatagattta atactaatct gtgcttgatg aaaattctca ctgtaaaaaa
    21301 tgctgaaaat caagtataat accataggaa caaactggaa ggtgaaaaag agcatggcac
    21361 aactattttt taagtatggc gcaatgaata gcggcaagac gatcgagatc ctaaaagtcg
    21421 cacataatta tgaagaacaa aataagccag ttgtcttaat gacaagcggt attgatacaa
    21481 gagatggttt aggcgttgtt tcaagccgaa tcggactgaa acgtaatgcg atgccgatat
    21541 ttgaagaaac agacgtattt gacgtaattc agaaaatgga aagcaaaccc tattgtgtgc
    21601 tgatcgatga agcgcaattc ttaaaaaaga aacatgtcat tgcttttacg agaattgtgg
    21661 acgaattgga tattccggtt atggcatttg gtttgaaaaa tgattttcga aacgagttat
    21721 ttgaaggatc aaaatattta ttgctttatg cagacaaaat cgaagaaatg aaaacaatct
    21781 gttggttctg ccacaaaaaa gcaatcatga atttacatta tattgatgga aaccctgttt
    21841 atgaaggcga tcaagtgcaa attggcggaa atgaagctta ttaccccgtt tgtcgaaaac
    21901 attactttca cccatcaatg attacagaag gagaataagc atgtacgatc aattacaagc
    21961 aattgaagac cgttatgaag agcttggaga acttttaagt gacccagaag tgatttctga
    22021 tacaaagcgt tttatgcagc tttcaaaaga agaagcaaac acgcgcgaaa cagtcgaagt
    22081 ttatcgtcgt tataaagaag tagtacaagg aatcaccgat acagaagaac tgcttggtga
    22141 aaaactggat gatgaaatgg ctgaactagc aaaagaagaa ttatctgaat taaaaaaaga
    22201 aaaagaagtc ttagaagaaa aaatcaagat cttgctttta ccaaaagatc caaatgacga
    22261 taaaaatatc atcatggaaa tccgtggagc agcaggcgga gacgaagcag cattattcgc
    22321 tggcgatctt ttcaatatgt accaaaagta tgctgaagca caaggctgga aaacagaagt
    22381 gatggaagct aacgttaccg gaatcggtgg atataaagaa gtgatcatga tgatcaccgg
    22441 agacaacgta tattcgaaat taaaatatga aagtggtgcg caccgcgtcc agcgtgttcc
    22501 ttcaactgaa tcgcaagggc gtatccatac ttccaccgca acagttgtcg tgatgccaga
    22561 agctgaagaa gtagagatcg atattgaaga caaagatatc cgtacagata tctatcatgc
    22621 ttcaggtgct ggcggacagc acgtcaataa aacagcatct gctgtacgtt tgactcactt
    22681 accaacaggg atcgttgttg ctatgcaaga tgaacgttct caaatcaaga accgtgaaaa
    22741 agcaatgaaa atcttgcgtg ctcgtgtgta cgataagatc cagcaagaag cacaaagcga
    22801 gtatgatgcc aatcgaaaat cagcagtcgg aactggggat cgttcagaac ggatccggac
    22861 gtataatttc ccgcaaaatc gtgtgactga tcatcgtatc ggattaacta tccaaaaatt
    22921 agatcagatt ttagcaggga aattagatga gatcgttgat gcattgatca tgtatgatca
    22981 aacatcaaaa ctggaagcta tgcaaaatgg gtaaaatgac ctatcgagaa gtcctgtcac
    23041 gggcttcttc ttttttagag gatcatggga aagaagcata tagtatccaa tttttatttt
    23101 tggaacgaaa acaatggaaa aaacttgatt ggctccttca tatgaacgaa gagatttcag
    23161 aagaagaaca gcggttgatc gaaaccgatc tgcgacttct gcttgcagat catccacctc
    23221 agtatttgct aggatatgca gatttttatg atcatcggtt aaaggtaact gaagcaacac
    23281 tgatccctcg accagaaaca gaggagttag tcgaatggtg tttagacgag acgcctggtg
    23341 taccgttaga agtgatagat attgggacag ggactggagc aattgctatt agtttgaaag
    23401 cagcaagaaa aaattggcat atatctgctg tggatctttc agaagaagca ttagaagtag
    23461 cgaaagaaaa tgctcagaaa gaaggaacga aaatcagttt ttatcatgga gatacattag
    23521 gaccagtaat ggatcagcaa tttgatgtga ttatttctaa tccgccgtac atcagtcgga
    23581 atgaatggga actgatggat gaaagtgtcc ggtcattcga acctaaaatg gccctttttg
    23641 ctgagaacga tggattagct atttatgaaa aaatagcagt tgaagcacca agcgtactaa
    23701 aatcaaatgg aaagattttt ttagaaatcg gatttcgcca aggtgaagca gtaaaaaaaa
    23761 tatttcagca agcatttcca gataaaaaag tggcgataaa aaaggatctt tttggcaatg
    23821 aacgaatgat ccaagtttat ggttgaagtt gtggatgaaa aattttttat ttgttttttt
    23881 attataaaaa aacacttatt tgacaaggat gtaaacgact ttttggttta tcaacaagtt
    23941 tatccactat tgacaaatag attatccaca aacagtggaa aacttgagga taaactcaga
    24001 caaaataaga atgtatgaaa aaggggttgt ggataaactg tggaaacgaa acgatatgat
    24061 gaaacagaat tgcaagaggc agccgaagca ctgaaagaag gagaacttgt tgcttttcct
    24121 accgaaacag tatacggatt gggcgccaac gctcttttac caaacgctgt caagaaagtg
    24181 ttttctgtaa aagggagacc gcaagataat ccattaatcg tccatgtcgc ctcttttgaa
    24241 caagtaaaag agtatgtgga caactttcat ccggcagcag agaagattgt gaagaacttc
    24301 tggcctggac cattaacttt gatatttaag atcaaaaaag acactttgcc ctcagttgtg
    24361 acgggaggat tatccacagc tgcatttcgt ataccggata acaaaaaaac actagaagta
    24421 atcgaacttt ccggtgttcc gcttgtcggt ccaagtgcaa atacgtcggg aaaaccaagt
    24481 ccaacgactg cagatcatgt atatcatgat ctacaaggaa aaattaccgg gattattgat
    24541 gatggagcta cgcgaattgg tgtagaatcc actgttcttg atttaagtga tccaacagcc
    24601 atgcctatga ttttacgacc aggtgcagta acgaaagaac aaatcgaagc agtcatcgaa
    24661 tctcctgttg ctattgatca gcatcttgta aaagaaaatg aaacaccaaa agcccctggg
    24721 atgaaataca aacactactc acccgataca cgtgttctga tggtcagaga tggcgattgg
    24781 tcaactgcag ttcaatgggc aaaaaataag aagatccgtg caggtgtcat agcaagcccc
    24841 gaaattgcgg atcaagtgcg gacagatact gcagctgttt atatgtataa cgacaactca
    24901 gtagaagcgg cggctaaagg attgtttgca ggtctaagag gattagatga accaacactt
    24961 ggattagatt tgatttttgt acaagtgtat ccagaaactg gattggggaa tgcttacatg
    25021 aatcgcttga aaaaagcagc aggacaaaat tattttgaaa aatgatcaat tggttattgt
    25081 cgcattcttt tagtatgata caatgctatc aagtaaatct tctatcagtg aacgaaaaac
    25141 taagcgtaac taatcgtaat agaagagata cgaatatttg gcaatttgtt tttgatggga
    25201 aaaataaaat tgtaggaaaa taaaaaggag ctgttgtttg tggtagatta caaaacgttt
    25261 gaccctgatc tttgggctgc gatcgccaaa gaagaagaaa gacaagaaca taatttggag
    25321 ctgattgcct ctgaaaactt tgtctccgaa gcagtgatgg ctgcacaagg aagtatcttg
    25381 acaaataaat acgcagaagg ttatccaggg catcgctatt atggtgggtg tgaatttgtc
    25441 gatatcgttg aaaatttagc gattgaccgt gcaaaagaat tattcggagc gaaatttgcg
    25501 aacgtccagc ctcactccgg ctctcaagcc aacacggctg cttatctcgc tttggtagaa
    25561 ccaggggaca cgatcttagg aatggatctt tccgcaggcg gacatctaac acatggatca
    25621 ccagtcaact tcagtggcaa aacctatcac tttgttgctt acggtgttga tccgacaaca
    25681 gaagttattg attataatgt ggtacgtatt ttagctagaa aacatcaacc aaaattgatc
    25741 gtagcaggtg ccagtgctta cggacgaacg atcgactttg cgaaattcag agaaatcgca
    25801 gatgaagtag gcgcaaaact gatggtagat atggcacata tcgcaggatt agtagcagca
    25861 ggacttcatc ctaatccagt tccttatgca gatatcacaa cgactaccac tcataaaaca
    25921 ttgcgtggcc ctcgaggcgg catgattttg acaaacgatg aagcattagc aaaaaaaata
    25981 aatagtgcgg tatttcctgg tattcaaggc ggccctctag agcatgtgat tgcgggtaaa
    26041 gcagtagcat tcaaagaagc attagatcct gcatttaaag aatatagtga acaaatcatc
    26101 gccaatgcaa aagcaatggt caaagttttc aatcaagcaa ttggaaccag agtcatttca
    26161 ggggcgacag ataatcatct gatgttgatc gatgttcgcg agttaggtat aaatggaaaa
    26221 gaagcagaga gcattttgga cagtgtgaat atcactgtaa ataaaaactc gattcctttt
    26281 gaaacactaa gtccattcaa aacaagcggt attcgaatcg gcacgccagc aatcactact
    26341 cgcgggttca aagaagaaga tgctgtaaaa gtggctgaac tagttgtcaa agcattacaa
    26401 gcaaaagatg ataacgctca actagacgaa gtcaaaacag gtgtacgcga attaactgaa
    26461 aaatttccgt tgcataaaaa ataaaaattt aaattccatt agtaaacaga aaagttcaat
    26521 gttgggcttt tctgtttatt ttttgggaaa acggattctt tgacaatatc ggtgatgctc
    26581 tctagtatat gaggtgtaat tttagtcttc acactaaatc tcagggagga aaagtcatga
    26641 ctgatcgtat ctcgttagaa aaggaagcag tagcattcag tgatgcaaat gcgcctcatc
    26701 cgcgtatcta tgaactagca ccggaagaag gaagagtttt actagaagaa gtacaagaat
    26761 cacctgtgaa caaatattcc gtcgatattg aagacttgat ggtggatact ggcgaatggg
    26821 gaaaactcaa tattcgtctc gtacgaccag aagggaatat ggataaatta ccagttattt
    26881 tttatattca tggagcaggc tgggtttttg gcagtgctca aacccatgat aaattgatca
    26941 gagaacttgc ggttcgtacg aattccgtcg ttgtctttcc agaatatagt cgatcaccag
    27001 aagcaaaata tccaacagca attgaacaaa attatgctgt gttgcaacag ctgaataaat
    27061 tagccaagtc aaaaaatctg gatctcgaga atctaatcgt tgcaggagat tcagtaggtg
    27121 gtaacatggc tacagtcatg acgatcttaa caaaagaacg aaagggcctc ccaatatcca
    27181 agcaattgct gtattatcca gtcacaaacg ctgattttga tacagattcc tatcatgaat
    27241 tcggagaaaa ctattttttg acaagagaag gaatgatgtg gttttgggac caatatacga
    27301 cgaatgaaga agagcgtgct gaaatcacag cttcgccttt gagagcaaat ataaatgaac
    27361 taaatgatct tccttcagca atgattttaa cgggagaagc ggatgtatta agagatgaag
    27421 gtgaggctta tgcgcgcaaa cttcgtgatg caggggttcc tgttacacaa gtacgcttcc
    27481 aaggaatcat ccatgatttt gtcatggtaa atgctatgga tcagacaaag gcaacgcgtg
    27541 cagcaatggc attatctact tcttggatca agaaaaaaga ataagctttg cttgtccgca
    27601 ggaaaacttc ttttcaaaaa cggattttct taggaaaaat ggcgagtttg actagtcaat
    27661 cacttgattt tgttttacaa ttaaaaagaa acgacagaac aaaggagagc ttaaaaaatg
    27721 ggcaaatttc aagtgatcga tcatccattg attcaacaca aactaactat tattcgggac
    27781 aaaaactgcg gaacaaaggt ttttcgtgaa gtagtcgatg aaatcgctat gttgatggca
    27841 tatgaagtat cacgggatat gccattggaa gacatcgtga tcgaaacacc gatcactgaa
    27901 acaacacaaa aaacgttgtc tggtaaaaaa gtagcgatta ttccaatctt gcgcgcaggt
    27961 attggaatgg ttgacggtat cttggaattg attccagctg ctaaagtggg acatatcggt
    28021 ctttatcgag atgaagaaac attggaacca catgaatatt tcgtcaaact tccagaagac
    28081 attgatgcac gtcagttgtt tgtagtagat ccaatgttgg caacaggcgg atcagcaatc
    28141 atggcaatcg atgcattgaa agaacgtggc gcgtcgaaca ttaaatttgt ttgtttggta
    28201 gcagcacctg agggagtaaa agctttgcaa gaagcgcatc ctgatattga catctataca
    28261 gcttctctag atgaaaaatt gaatgaacat ggttatatcg ttcctggact aggggatgcg
    28321 ggtgaccgtt tgtttggtac aaaataagta aatacgaaaa agggtgtgac acaacttttg
    28381 tcacatcctt ttttggtcat ttttaacgtt tgatgcagaa cagctttttc acagctttcg
    28441 tttgcatata aaaaaaccat cccaaaaatg cagagatggt caaattcaaa tcttttttag
    28501 ttctttgtag ttaacgatta ctacaccctt actttgcgtc atcaagttcg cattttctgc
    28561 agtttcatca atctcaacaa cagcggaatt tttcaattgc tttgaaatcg ttccagtttg
    28621 cgacttgccg tgaattgtga acattacgtg agtattgatc ttgaattttt caccaatttc
    28681 cgatggtacg atagttgtat caaattttcc aaaagttgcc attttcataa aacctgcctt
    28741 tcacatcaat tataccacgt tttatgaaaa atatcatgaa attaaactaa ttttccatcg
    28801 gactctttac aatcagccaa atagagtata taatttcttt tggataaggc cagcttagct
    28861 cagttggtag agcaacgcac tcgtaacgcg taggtcgtag gttcgatccc tatagctggc
    28921 attaaaagag aagacagata tatcaaggat ttttaatcca ttgatacatc tgtctttttg
    28981 tttaaaggaa ataaagtatg ctttcttagc aatagagcat gtattatttc tataaaaatt
    29041 taggagaata aaacgactag caatatagct gtgaaatcaa atatcacatg aactgcccaa
    29101 cttgtaatga tcgagttgct tttaatggca gcttggttga ataacaatct tgccacccct
    29161 tgtatcaaaa ggatgtgtgc tacagtgtgt aaaatatttc cattatcata agtactgtaa
    29221 tggataaatc caaagatgat tgctgacaag aaactagcga caggaatgct gaacttacta
    29281 gaaaaaatag cgagaaaata cactgagaac aattcttctc caagtatcat gataggcaaa
    29341 ataataaata ataacgggct gaaagcctca tttattggat tgcctgttaa accccatttc
    29401 aaaatttgtt gcaaaaatac cgagactgcg gcactgatta ctacattgat aaaaaaatat
    29461 ttggctatat ttttgacagg agcgacaggt ttcgagaaca tttttttcgt atctttttta
    29521 ccaaaaacaa gtgtaaatgc tataaatcca gtgatagtta aagaaaaaat gctaataatg
    29581 gatggcgcaa atagcaaccc taacaaaccg agtacaacga caatagtgcc caatatccct
    29641 tttttaagtt ctattttttt tcctaaaatg ttattcatga taataactcc tactattcag
    29701 tttttgaaga tagagtcagt atacatgaag agttaaaata ggcaatgttt ttttatggat
    29761 atttgtattg ctaaaaattt atcttatata ttcattttga ttatactact tttatttgta
    29821 aaaattttct agcaaaacca agtgcttgca actgattaag cgtacaattt tttagatgtt
    29881 ccgagcgtac ttcaatcatt tcaaaggagt tgctacttaa atcgatattt tttaaatcag
    29941 tatcaaaaaa gctagcattt tctaaatcat tagtagaaaa cattatttta gtgagggaac
    30001 agtctacaaa atctgcgctt gataagacag agtgctcaaa ggtactttct tttatgtttg
    30061 aaccagataa atttacatag tccattttac aattctcgaa taacgtgttc ccaatataac
    30121 tttccatcaa tttagtacct gttaatttac aattgacaaa tttcacgcga tgcaacatgc
    30181 tttttgtcaa tatacagcct gaaaaatcac agttttcgaa tatacagtcg gtaatctcca
    30241 ttctttgaaa atcattggaa gcaaaaaatg catacttgaa aatagatttg taaataattg
    30301 gatggcagat tatttggttt tcgatgacct ctttttgtga aaggatatta tacagttcaa
    30361 aatcttcgtt ttcatagata tgaaaaaagg atcctttatt caagcttgct tcattaactt
    30421 taggaaaaat catatttaat tacctccatt ttcataaagt catattaaca aaagctaaac
    30481 caacttacat tagcagaaaa tgagataggt tttcgccttt ttcgataacg atagtgtcgc
    30541 tatctcttga aacgttctta gaattgccct gagagacaca aattaggcgt aattctaata
    30601 aaaatcttag aataaaaatt tttactgatt taatttagaa t
//
LOCUS       DAKBVG010000009        27852 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000009 DAKBVG010000000
VERSION     DAKBVG010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 27852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27852
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.009"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(<1..334)
                     /locus_tag="OUD09_000349"
     CDS             complement(<1..334)
                     /locus_tag="OUD09_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9691655.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFE"
     gene            complement(509..2644)
                     /locus_tag="OUD09_000350"
     CDS             complement(509..2644)
                     /locus_tag="OUD09_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCT9691656.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(2727..4382)
                     /locus_tag="OUD09_000351"
     CDS             complement(2727..4382)
                     /locus_tag="OUD09_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCT9691657.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKNLLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(4384..4698)
                     /locus_tag="OUD09_000352"
     CDS             complement(4384..4698)
                     /locus_tag="OUD09_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCT9691658.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            4868..5128
                     /locus_tag="OUD09_000353"
     CDS             4868..5128
                     /locus_tag="OUD09_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HCT9691659.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            5142..5456
                     /locus_tag="OUD09_000354"
     CDS             5142..5456
                     /locus_tag="OUD09_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HCT9691660.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(5941..6495)
                     /locus_tag="OUD09_000355"
     CDS             complement(5941..6495)
                     /locus_tag="OUD09_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCT9691661.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(6621..8549)
                     /locus_tag="OUD09_000356"
     CDS             complement(6621..8549)
                     /locus_tag="OUD09_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HCT9691662.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(8855..9571)
                     /locus_tag="OUD09_000357"
     CDS             complement(8855..9571)
                     /locus_tag="OUD09_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HCT9691663.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(9572..11395)
                     /gene="glpO"
                     /locus_tag="OUD09_000358"
     CDS             complement(9572..11395)
                     /gene="glpO"
                     /locus_tag="OUD09_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HCT9691664.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(11409..12905)
                     /gene="glpK"
                     /locus_tag="OUD09_000359"
     CDS             complement(11409..12905)
                     /gene="glpK"
                     /locus_tag="OUD09_000359"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCT9691665.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(13041..14501)
                     /locus_tag="OUD09_000360"
     CDS             complement(13041..14501)
                     /locus_tag="OUD09_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCT9691666.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(14491..15786)
                     /locus_tag="OUD09_000361"
     CDS             complement(14491..15786)
                     /locus_tag="OUD09_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCT9691667.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVKEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(16118..16795)
                     /locus_tag="OUD09_000362"
     CDS             complement(16118..16795)
                     /locus_tag="OUD09_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HCT9691668.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(16825..17916)
                     /locus_tag="OUD09_000363"
     CDS             complement(16825..17916)
                     /locus_tag="OUD09_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HCT9691669.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(18017..18451)
                     /locus_tag="OUD09_000364"
     CDS             complement(18017..18451)
                     /locus_tag="OUD09_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCT9691670.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(19035..20492)
                     /locus_tag="OUD09_000365"
     CDS             complement(19035..20492)
                     /locus_tag="OUD09_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCT9691671.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENL
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIEDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(20489..22384)
                     /locus_tag="OUD09_000366"
     CDS             complement(20489..22384)
                     /locus_tag="OUD09_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCT9691672.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(22442..24217)
                     /locus_tag="OUD09_000367"
     CDS             complement(22442..24217)
                     /locus_tag="OUD09_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HCT9691673.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYLVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESSEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(24267..25763)
                     /locus_tag="OUD09_000368"
     CDS             complement(24267..25763)
                     /locus_tag="OUD09_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCT9691674.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     EIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRIWKEEDCAPVMLDGDEQYLQANTVDYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(25957..27579)
                     /locus_tag="OUD09_000369"
     CDS             complement(25957..27579)
                     /locus_tag="OUD09_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCT9691675.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
BASE COUNT         7902 a         5992 c         4631 g         9327 t
ORIGIN      
        1 attcaaaaga tttatttact tttttgtata gatttcgatt gttccaccta gtcaatacaa
       61 atgaggtaat caggaaactg aaaaaaatca atacaaaaaa gccgattaag aaatacagca
      121 ttttcttttc tgataaaaaa gcgtacaagg aaaaatcctt ggctctttct ggtctcgtta
      181 atgggaaaaa gacccatata aaaggaagaa caacgatcat ccatcctaaa agagccacta
      241 acgtttgcca aatctttaac cagtaattac cttttgaaaa atacttatct ttcaccaaaa
      301 ttgatgcaac ttcttctctt gttacttttt tcatatcctc acctcacgtt tttttgttac
      361 gacgtaggag tttaccatac atttcatttc ttttcacata aagtgttcct aaatttttcc
      421 taagaaaaag aacaaaacat ttctcatcag caaatgaaaa atattttgtt ctttttatca
      481 gttgttcttt ttatttttta tctcttaatt atgttgctct ttccaatacg acaatgcttt
      541 tcattcctct agatatcgcc gtgtacattt gtctgcgtct tctttgctca tctccatcgt
      601 tttcaaatgg atataagatt acatgatcaa attccagtcc ttttgctaga ctcaaaggaa
      661 tgatttggca gttttctaat ccatatgaat tttttttaat cgattcttgt agcgtttggg
      721 tttcctcttc tgtttgggtg ataactgccg ctttttctcc ttttagagaa accaaaatct
      781 cagctagtat tttcaacaat tcttcagagg atcgaaaaga gcggtattcg ggttcttttc
      841 cttgcggtcg gatcgatacg atgtcgatct tctcttgatc cacagcatag gttttgaaaa
      901 gctctgtgat tgctccgcta gaacggtaac tatttctaag atcataccga tggatcggca
      961 gatggtagtc atcgaaacaa cgcataatat cagcaaaagt agtggaagaa ttaaagattg
     1021 cctgattctc gtcacctact aaagtaaagt gagtttttgg aaataattcg atcaatagac
     1081 ctaattgtgc cagggtataa tcttgtactt catcaatcaa tagatagcga aatgctggta
     1141 catttacctt ttcaacaagt aaatgacgaa tagctaatat aatgactgct tcatcaacag
     1201 tacgtgtggg ttgccaagca taagctgagc catatcgttt ttcataaatt ctttcaaaca
     1261 actgatgctc atccacccat gccatttctt cgatttgtct ggttattttt cgatattttt
     1321 tttgtaaaag tttccttgca taagctacaa tactttgttc cgaatcatca gaaatcaatt
     1381 taccgaataa ttccaactgc ctatcttctg tcaacgagag tacctgattc tgcacagcag
     1441 gactttttgc ttgtttcaaa agatgattct tccattcgct catcagtgcc tttttcgttg
     1501 cttgaagcct atcgatcaat ttaggatgag caggaacttt ctcatatatt ttttcgatcg
     1561 tttttttaga aaggactgtt tttccatttt tagtaagtcc acggataaaa ttcggatgat
     1621 ctaaaaacaa aggatctgat tgttttagat tatcgataaa ctttttgcta cgcaaacgtt
     1681 ctgtctgctc cgatacattt tctcccgtga tacgtttgaa ataagcctct tcaccttcta
     1741 tttcctctgc tgaaagctgt gaaaccagct gtatgatggt tagattcagt ggatttcttt
     1801 cacctaatga tggtaggaca ttcgaaatat agtcgataaa ccgatgatta ggtgatagaa
     1861 tcagcaagtc atcggatgtc atcgtatctc gatataaata gagcagatac gcaatacgct
     1921 gcatgatagc agaggtttta ccacttcctg ctacgccatt taccaagata ttttgacttt
     1981 tcacatcccg aatgattgtg ttttgttccg attggatcgt agcagtgata gccttcattt
     2041 cattggtctc atcttggcct aagacttcta ataatacgtc atcttgaata gctacagacg
     2101 tatcaaaaaa cttgaccaaa cgatctcttt ccaaaagaaa ttgtcgtctt tctttaataa
     2161 ccgtatcgat tttattttga cgaaccgtat aagaagatgg accaatcatg ttattgtaaa
     2221 aaagttcggc aattggtgaa cgccaatcat aaacaagatt ttcatctgca tcatctgtga
     2281 aatttgctgt accgatataa aaggattctt ttggctcatt atccagaaag tctacaacga
     2341 cttttccaaa gtagggactt ttcaataaac gttcgatctt tgctagctga ttttctgcgt
     2401 ttttctgttt gatattcatc tgatcgattt ctctattttt catttcaatc atcgaaaaag
     2461 tatccaggtt atctaagtaa ctgtcaaaat tcaatcgaag ctcgccagcc atttctttca
     2521 acgactggtt tccttctatt tttgccgtat ccatcgattc ccgcagttcg ttttttttat
     2581 caattagctt ttgatacacg ttcttcagat atgcttcttc taactgtttt tgtgtgttga
     2641 ccatacacat tctcctcttt ttttaaagtg gtggaaccga ttagtttacc tcttttaaga
     2701 gtcgaatgca agctttttaa cgagtatcat ttgttaggat atatcagttc ttcaggtcgg
     2761 acagtttgat ataaagaaaa tgcaccatca aggtttttta cgtggtagcc agactgcttc
     2821 aagattcttt ccgctagata gctacgtaat ccgctatgac agctgacgat atattcttgt
     2881 tggctgtcta attcatttaa tcggtcccga agttcattca aagggatatg tttggcttcc
     2941 ttgaatttgc ccttttccaa ttcttctgct gttcgcacat ctaataaatt ttttcctttt
     3001 gctagttctg tcggcaattc atgccattgt acggtttcac taagcccttc tgcaatgttc
     3061 attgctgcat atcctagcat gtttactgga tctttggctg agccaaatgg cggtgcatac
     3121 gtcaattcta attccggtaa atcgaagatc gtcaaatgcc ctttgattgc tgttgctaaa
     3181 atatcgatcc gtttatccac accttttgcc ccaacacctt gtgcaccata aatctcgcca
     3241 gtctctggat ggaagatcaa cttcagtaaa atatctgttg cttctggata atagcttgca
     3301 tgatcttttc ctgatacatg aatgacttca aaagacagtt cgttttcttt ggctgttctt
     3361 tcacttaaac cggtggatgc ggcagtcatt ccaaacgttc gtataattgc tgttccaata
     3421 ccgccttgat ttcttcgaga aattccggaa atcgtgtccg caacttgtct accttgacga
     3481 ttggctggtg aagcaagaga aataagtgca tcttttccag tgatttgctg ttttacaacg
     3541 atagcatccc ccaccgcata aatatcagga atattcgttt gataacgttc gtctactagg
     3601 atacccccgc gtatgcctaa ttcgattcca gcggtcactg ctagctggtt atcaggacga
     3661 acaccaatgg ataaaataac taaatcagca gggatggtac gcccatcatc cagtcgcaat
     3721 cgtttcccgt ggtcttcaaa tccagcgaca gcatgagaag taatcacttc tactccttga
     3781 tgagacaatt ctttttctat aaaagctgcc atttcttcat ccagtgttgg aagaatatgt
     3841 ggtgcttgtt cgatcaccat gacttctaaa cctcttcttt ttaggttttc tgccatttcc
     3901 aatccgatga atcctgcacc aacgatcacg gcacgctttg gctgtttttc taatgcatgc
     3961 atcactttat cgatatctgg tacatttcta atagaaaaaa cattgtcggc ttctgttatc
     4021 cctggaatcg atggaacaac tggttttgca ccaggagata aaaccaatgc atcgtactgt
     4081 tcaatgtgtt tttgaccctc atgtacgact tctaccgttt tgttttcggg aaagatggct
     4141 gtcacttcat gatttggacg cacatctaaa caaaaacgag cggataatga ttctggcgtt
     4201 tggacaagaa gattttcccg ttcactgatt tctcccgaaa gataataagg caagccgcaa
     4261 tttgcaaaag aaacatacgg tcctttttca aaaacaacga tttctgcatc ttccattaat
     4321 cggcgaagcc gtgtagcagc agacattcca cctgctacac ctccgacaat aacgattttc
     4381 atcttatttc cctcctactg ttcgatcaaa ccattgattc atgccaccac gtacattcac
     4441 tacatcataa ccagatttct ttaattcttt agctgcttgt ttactcctca tccctgattg
     4501 acaaatcaca tagacggtct tttctttgtc tccatcatat ctgtttatac tttgtaatgg
     4561 aacattttta gctcctttta tatgcccgcc ccggtattct gcaggtgttc taacatcaag
     4621 taacgctgga ttttccaaaa gcttttcctt taattctggg actgttatcg attttattga
     4681 ttgaaatagc aaattcattt tctgcacctc atttacattt ttataaatac ctcatggggt
     4741 atattaaaca ggagctttat taatatgtca accagaatga ctcttcattt ttttcactac
     4801 tttttatatt tgcataaaat accgataagg ggtattatct aattataaaa atagaggaga
     4861 atgtgttatg gagattgata ataaaaatat cctgaaccgt ttgaaacgta cagagggaca
     4921 aattcgtggt atccaaaaaa tgatcgaaga cgaaaaagaa tgtatggatg tcatcactca
     4981 attaagcgct gttcgttcta gtattgatcg cgtaatgggc atgatcgttg cagataatct
     5041 gaaaaattgc tttgagaaac ctgatagtaa tccagaagaa caagcggcta aactagaaca
     5101 agcgatcaag atgattatta aaaagtaaag gaatgatttc aatgaaagtc gttttccata
     5161 ttgatgaatt agaaaaatgg tcagaaacag ggaaaaacgt aaaaaacctg ataaaagctt
     5221 ctccagaaac gacgattgtc gtatctgtaa atggcatcgc gattaccggt tatctagact
     5281 ctgccaatgc agaattctta gatttgcaag gagtcgtttt tcacgcttgc gccaatgcta
     5341 tgcgtgctaa tcatatttca gaaagttctt tacctgaaca agtaatcgtt gtaccagcag
     5401 gtgtccttga tttagttgaa ctgcaatccc aaggttatgc ttatatcaag ccttagtcag
     5461 tagaggttgc gacagaagtg atcagcttca agaaatttcg gcttattttt cgaagaagct
     5521 acttctggaa aaccgtgaat caaaggttgt gaaaacgctg tcctgcatca agtaataaga
     5581 acagccaaac aaaaatagct tctcatattt ttcgtttggt tgggcttatt accgcagatg
     5641 caagcttttg aacaccgttt aaggaagagg ttgtgaaatc agcggtttga cctaagtatt
     5701 agagaaaaaa acgggaaaat agctcctcat attttgccgt tttttgagct aataccgaag
     5761 gtcagctgat tgaacaccgt ttaccaagag gttgtgagcc taccatttaa ctccgaatca
     5821 caagaaataa tttcaaaaac aacttctcct gtttttggtt tggttggctt attatcgaga
     5881 agtagctact gggagtctat ttatcaaaaa aaggaatgcg acataggtca cacccctttt
     5941 ttattttctt tgaaacatct ttcggtactc gacagcatac aagacaaaag attgtaaaag
     6001 accgtaagtg atgacggcaa aaatgactct cacccacagc gttggtaatt ctgggaaaag
     6061 cagcaaaagg atcatgccaa catagatgac agcagtcgct actttgccat accacttcgc
     6121 tccacttaat ttgatatttt tagatagccc gattaatcca gctacaaaca taaagccttc
     6181 ttttatcaag aatagaccaa acaacagcca aagccacggt cgataccatg ccatagacaa
     6241 aatcagaaaa agttgtgtca atttatctgc aaacggatca agcaccttcc ctaagtctgt
     6301 tacctgatta tatctccgag caatgacccc gtctagataa tcagacaaac ctgagacaac
     6361 aatgaccgct cctgctatat aaaatgactg tctcagcaca aaataaagat aaatcggcag
     6421 caaaaaaata cggaatatcg ataaaagatt cggaatcgtt tgtatttctt tacgccagtc
     6481 tgtgcttaat ttcattttat cgccctacct cctcagcact cttttctttt tattttagct
     6541 tacacgggga ataaaaccaa tgatttcact gtttttattt ttctttacag ccactgatat
     6601 tatgagctaa tttgagataa tcacttgctg ttttcatcgt ttactcttct ttcttcctgt
     6661 cgtttgcgca tttcttttac tagtacttta tgagcttttt ttgatcctcg ctctaggtca
     6721 cgctgccgtt tacgtgtttt ataatcgata aacagactgt ctaaatcgta acctgtcggg
     6781 tacagatctt ctgctgaacg caccagacga acacgctgac gaggcacgtg gattattttt
     6841 ttgtctaaat atacatcaat cgtatcttca cctatatctt cataaactaa cgcttctttt
     6901 tggtattcat tgacaaatat ccgatcacct tttgcaaaga gctggcgttc ttttttattc
     6961 tccttcctat ttgtaactgc tgtcttagga agaaattctt ttttctgtgt ccgataggct
     7021 ttttccgcca agtaacgctc tgcttgatgg atcaatttca tcgacatatc cattttatga
     7081 gcaatccaca aagcttggct ttctcctgtt tctccaaccc gcaactgata tttaggacga
     7141 agtgcttccc gatcgaaagc catagcagcc ggcacaaaat cttcatgatc tccggcgaat
     7201 ttcttgattt ctccataatg ggtcgtggca atgatcaatg ccccttgctc atacatagat
     7261 tccatgattg cgatcgctag tgcagcccct tcatttggct ctgttccact gccaatctca
     7321 tctaaaagta ccaacgtatt cctttttatc tttttcaaaa tagcagcgat gttttgcata
     7381 tgaccagaaa atgtactcaa cgcattttct aaattttggt ggtcaccgac atcgacgaaa
     7441 atctcatcaa agacagcaag ctctgtccct tcttttgccg gaacttgcat accgaacatc
     7501 gccatcaaag tcaataaacc gactgtcttc aacacgactg ttttgcctcc ggcatttgct
     7561 ccggtgataa tcaagcctcg atagtcttta ccgatttcaa catccaatgg taccgcatgc
     7621 tcaggtaaaa atggatgtct gccttgcttg ataatgatat gttccgattt attgattcta
     7681 ggtgtgcttc cattgatttc tcggctaaat tttgcccgag caaagatgat atctaacacc
     7741 gtgatcgttt cgatcaataa atcgatcact tcttcgtttt ctgcgatagc accagtcaat
     7801 gccgccagga cttgatattc ttcactgatt tcttcagctt ttaacaactg atagtgttca
     7861 ttcaatttag aaatcacagt tggttcaata aaaaccgtcg ttcttttatt tgattcatcg
     7921 ataatcgttc catctatttt atttttatag ctggctttga taggaatcgt atagtattcg
     7981 cctttttgaa caatcattgc ttcttggatc atttctttgt ttttcggatg acgcaagaat
     8041 ttcagtaatt ttgactggat ttctttttcg attagttgta attgtttgcg tactttacgc
     8101 aaattacgtg acgcgtcgtc actgacttta ttatttttta ttttctggtc aatcaactct
     8161 tctacattta tcaaatcagg aagatgtttg ctataagcaa acaacaaggg tgtctggtat
     8221 tgattcttat caaaaaactt tgtgatcatc cgactgcttc taagaaaatc cgcatattcg
     8281 atcaaatcgg ttggctgcag aatcaaccct tttttgactt gttctgtcag tgcatcgatc
     8341 ctcggcaacc ccataaatgg aacatgctga ctgctttcta agatcaagcg tgcttctctt
     8401 gtttcttctt gacgatccaa gacagtttgt aggtttgtcg agaccgtcat atcgctgatt
     8461 cgttttttgc tatagtttcc aattgctctt gcttggattt cctttttgat ccgatcaaat
     8521 tgtaattgac taatcatttc gttgtccata ttctctcctt ttcttgaaga gatacctgtc
     8581 ccaaaataaa taagggccta gagataaata aaaggataga acacagctta aaaaagtcat
     8641 gtcctatcct tattagaata caaagatatt gttttcctga caggtaccgt ttcataagca
     8701 caccaaataa actttgttta ttggctattc gacttactga ttagatacca gctacactca
     8761 caaaacatac cccattcttc taggtcttat ttattttcca cttaactata gcaagacaca
     8821 ttttggttgt caaactttag tatgttttat agatttacac cattaatgaa tagacaccta
     8881 ccgcgataat tgctccgatc attggacctg cgattggtac ccatgaatat gcccaatctg
     8941 aagttccttt attagctatt ggcaataccg catgtgctaa tcgaggacca aagtcccgag
     9001 cagggttgat tgcatatcct gttggccctc ctaatgaaag accgatggct aagatcaatc
     9061 cacctacagc gaatacgttc gttccaggtg caaattcgtt ttgtccaaaa gctagcaacc
     9121 caagaactaa tacaaatgta ccaattaatt ctgtgatcac atttgctgga taattccgta
     9181 tagcaggacc tgttgcaaac gttcctaaaa tcgcgctttc atctttcgtt gcattccaat
     9241 gtggcaaata agaaagccaa acaagaatgg cccctgcaaa agctcctaaa acttgcgcaa
     9301 tgatgaaagg aacgacaaga ttccaactaa ttgcccctgt catcgccata gctagagaga
     9361 ctgcaggatt caaatgagct gggcccatga atccagaaat atagacagcc attgttacag
     9421 cgagtcccca accaaatgcg atgacaatcc agccagaagc ctgtgcttta cttttgttta
     9481 aattcacagc cgcacacaca ccatctccaa gcaaaactag gatcagtgtc ccaataaact
     9541 cccccatgat ttgagtcata tcactgttca tttatttctc cccctttagt tcccgcagat
     9601 ctgattctgc aatcaatgct tctaaacgtt tagtctgttg cgccttctcc tcttctgtcc
     9661 attcaaaata tccagcaatt gcatcgatca caggttgctt gatctcatca agctgatctc
     9721 gttcaaacaa cagatgattt gtacgacgaa tgagataatc gcctggggcc aaaaccattt
     9781 cttcttctaa accataacgt aaccgagctg actcagcgag actcaaacca ggataaggtg
     9841 ccatttcttt tgctaattca aagatacgtc gggcattagt cccgtaaaaa tcagcgatat
     9901 aagtagcatc ttcttcctct aaaccggctg ctactccttc ttttgctaat tctgtcactg
     9961 tttcttctaa tttagttgga tcgaagtttc cacctgaaat ctgatatttt ttagagtcga
    10021 tttctttcgt ctctattccg tattcttctt ttaataattg gcgtatcaat cgcaaagctc
    10081 cttctgccat cttacggtaa tcggtgattt tcccaccaga taaagtcagc aaaccatctg
    10141 gttctctttc taaagaacta cctctggaaa tcgtagaagg tgctttttca tcacggctgt
    10201 tttccaaatg gttcaacacg tcttctactt cagcacgaga aactttgttt tccttatatt
    10261 cacttactgt atcgacaact ttattgaagc tcttgtctga aatcgatcca ttatctccac
    10321 cattgtaatc agaaccagaa ttaccaatca acagtggacg aagtcctgcc cagcttgctt
    10381 cgatatcatt caatgtgata tttgcttctg gatagcgatg attgatcaca tccaatagat
    10441 aatctacatc ttcttgtgtg actttcggat ccgtaaaatc tccttggtaa tccgtatctg
    10501 tcgtaccaaa gtaagtcttg ttttctcttg gaatagcaaa aaccatccgc ccatcttgct
    10561 ttcctgtatc gaagtatgtg ggttgcggta caggcagttt tttcgcatct acaactaaat
    10621 ggatcccttt agttggacgc attttaggag agactggacg cgtaaaattc aagtttcgta
    10681 ctttatctac ccaaggacca ctcgtattga tcactaattt tgctttgatt tcgatcactt
    10741 catctgtcag caggtcacga gctttgacgc caacgatttg atcgccatca tataaaaagc
    10801 caaccgcttt cattttactt actagataag ccccatcttc ggcagccttt ttgatattat
    10861 caatcactaa acgggcatca ttgttgcgga aatctagata gacacccgcg ccttttagcc
    10921 cttctttttt caaaaacggt tctctttcca atacttcttc tggtgtcagc gtataattct
    10981 catatttggt tcctgtcaca tttgccagtt tgtcataaag gtccattgct actttgaccg
    11041 agaacatatt gaaggttgtt gctccttcgc cctcatagat tggcaaaagc attggatctg
    11101 gttttgggat gtgtggggca atactttgta cgactgcacg ttcaccaact gtgtcagcta
    11161 ctacttccac atcaaatgtc ttcagataac gaataccgcc atgaacaagt ttggtcgagc
    11221 gagaggaagt cccttctgca aaatcctgca tttcgatcaa tcctgttttg attcctgatg
    11281 ctgctgactg gactgccaca ccggcaccag tgattccgcc accgatgatc aacagatcca
    11341 attcttcttg ctgcattttt tgaatgctat cttgtcttgt cttgtttgaa aacatatgga
    11401 tttcctcctt attcaaatgc ttgggtagct tttactgctt ttttccaacc gttgtaaagt
    11461 ttttctcggc gctcttcctc catttgaact tcaaatagct tgccagcttc ataaaattcg
    11521 cggatctcgt ctgtatcttt ccaataaccg acagctaatc ctgctaaaaa tgctgcccct
    11581 agcgccgttg tttccaagtt ttctgctcgt tggatcggta cattcaagat atctgtttgg
    11641 aattgcatca gatattcatt gtttgctgca ccgccatcta ctttcaatac aggaatcttg
    11701 attccagtgt catcttgcat cgtatcgatg atatctctca cttgataagc aatcgactgt
    11761 aaagtagctt taatgatatc ttcttttgtc gtaccacgtg tcaagccaaa catagcccct
    11821 cgtgctgcct gatcccaata aggagcacct agcccaacga atgcaggaac aacataaacc
    11881 tcatcttcgc ttgtagagcg tttcgctgcg tcttctgaat cgctagcttt ttgcaacatt
    11941 tgaagtccat ctctcagcca ttgaatcgaa gaaccagcca caaaaatact tccttctaaa
    12001 gcatagtaaa cctttccatt tatgccgtaa ccaattgtcg tcaataaatt atttttggat
    12061 aattgcggtt tttcaccggt attcatgaca ataaatgaac ctgtaccata agtattcttg
    12121 accatgcctg gttcgaatgc catttgtccg aataatgcgg cttgctggtc tcctgccatc
    12181 cctgaaattg gtgcttcgct tccataaaaa tgataacctt gcgtataacc ataaacttct
    12241 gagttagaag tgacttttgg taacatcact cgaggaatat ttagtaaatc taaaatctcc
    12301 tgatcccaat ctagctgatg gatattgaac aacatcgtcc gactggcatt cgagtaatca
    12361 gtaacatgtg cttgtccatc ggtcagtttc catactaacc aagaatcgat cgttccaaac
    12421 atcaattcgc ctttttcagc tcgttcttgc gcgccttcta catgatcaag aatccagcgt
    12481 atcttcgttg ctgagaaata ggcatcgatc actaaccctg ttttttcgtg gaaaaattct
    12541 tgatagcctt cattttttaa gttatctgca attccagctg actgtctgga ttgccaaaca
    12601 actgcatgat agattggacg tcctgtatct ttttcccata ttactgttgt ttcacgctgg
    12661 ttcgttatgc caatcccagc aatctgattt ggtttgatcc ctgattcgat gaacgctccg
    12721 gcaataactg actgtacaga attccagatt tcatttgcat catgttccac ccaactttct
    12781 tgcgggaaat attgcgtgaa ctctttttga gaactgccga tgtgtcttcc tttcttgtca
    12841 aagataattg ctcgtgagct agttgtacct tggtcaatcg ccatgatata cgttgattct
    12901 gtcattgtga ttccctccat tatatgcaaa cgctttcttt aactactctt atcatactac
    12961 tgttttaaaa aaagaaactg tcaaaatttt attctttttt tctttttttg acagtttttt
    13021 attatgaagc tattttttaa tcaattcttt tcttttttca gcgtctctat tcttcttttc
    13081 aatcgaataa tgtcgtaagg tgacgcaaat tctgacagat aatattcaca ctgttttttg
    13141 tttctctggc aaaatgtgat caccaaatca tattctttac cttcttcata acttccaatc
    13201 tcaataccgc cgattcccct cagttctctg atcagatatt gttgaaaagc aatacgataa
    13261 atcggcagac tttctaaatc gattccaatc actactggtt ttgcaatata aaaatcgatc
    13321 atcaaaagaa cattggcata ctggatcatc aggtaactaa gctcactatc ttcagctctt
    13381 ttcctcggca attgctgcac cgctgtttcc tgtaatttcg ccagtaactg tgttaaggaa
    13441 tgcccgagca ttttctgctg ctgttccaaa agccgatcac gctcatagac ttcgatcttt
    13501 ccaacaaaga aaaaccattc attgttgatc tgggaaattt ggtagcctac tgccttttct
    13561 tctttgattt tcaaacgacg aggacgataa tgccataaca tcgtttcccg aatgtaagta
    13621 tctaaaacag cggttggtaa ctttttgctc cgtgtcaaat catactgata aaatatttct
    13681 tcgtctacta tcgaaaaaga aacaaaaaag ctgtaaaaca tcattgtttc gtatacattc
    13741 agttctgctg ctgttcgact aagatgcatc acgatgattg gatcgattgc ttgataaagc
    13801 cggtccgact gatagagtaa tttcttttct ttcaaagttg cgtacgtact tttttcattc
    13861 aatagtcttt ttttcgtaat cccgagccag caagcaatct gtgcttctgc tgatgccgta
    13921 aacgttgttt ctaatacacg gtccaatcct agaatgaacg gtcttttttc tggtgtattt
    13981 tgcaaaaaaa gcggtcgctg atcttccggg atatattgga atagttcata ataaaaatat
    14041 cgtatctgta gctcttctcc gtataattga ccgtttttga tttgtaatcc aaattcctct
    14101 aagagctggt tcaattcttt gatctttcga aaaacagaag actcactgat attaaacgct
    14161 gtcgctaatc gaacgatgga tacttgctga tgttctaata aatagacgag catcttaaac
    14221 ttcaagcttt cctgcaataa aaacaataga attttttcca aacttacatc ttcgtccaac
    14281 acaagcagcg cctgatactc atttcgctgt acttctactt ttttttccat cattcggcca
    14341 atcaattcaa tatcttccaa atattgatca aacgaggctt ttgacagttg gacatgctct
    14401 ctcaaaccat tgatagaggc tgttccccct tgcagaacaa ggtttcgcaa aatcaggatc
    14461 tgtaactgct cttttctttc taataatgct tcaatcttca taggcagtat tcgccacctt
    14521 tgaccacacc atatctggca cacggtattc tggctggaaa aatagctcat gttcttctaa
    14581 ctgttcaaac gtccatcctt cttttactgc aattgtcagt tggtcaagaa ggtggaacat
    14641 ctcttttttg ctttggagct gtgctccaag aatctttttt tcagaatcaa atagaaattt
    14701 gagtttaact ttgctttgat ttattgctgt aagcttgtag tctacgtatc ctgtttcaat
    14761 tttttgggga taaaaaatgc tctcttcttc tgtcaaacct acgcttccta agaaatagcc
    14821 aaaccactga ttgccgactg gacgataggt tccctgatcc tttgtctgtg agccactaat
    14881 tgtcttgctg acatttcgtg ctgtacggac agcgttattt actagagaaa catacatttt
    14941 ttcatttgtt ggacgaaaag ttacttggat cgcatcgcca atcgcataaa cgtctttttg
    15001 agacgtttgc aagtagtcat ctacccagat cgttccatca tcattcaatg ttaattgttc
    15061 ttcccacatc tgattgtcgg ggcgagaatg attagccagc aaaacaagat catagttctc
    15121 ctttcttttt tccgtctcta caatagcggt ttcattttcg gtcaaagaca caacttgttc
    15181 ttctaaaaaa agtgaaagcc cttgattttt catttccttt actaacggtt ctgtcatttc
    15241 aggatctaga taacgaaaaa gaattgactt tcggctttcg taaagatgga tttgtttttt
    15301 tcttgatgcc aacccttcag ctatttcgat tcctgcttgt cctgcaccaa taattgcaat
    15361 tttctttgca ttcatgatct tttgctgaag agccgttacg ttttgtatct ttttaactgg
    15421 gtgaataagc gttgattctg cagaaatatt tcgaaacttt tgatttgaac cagtcgctag
    15481 aatcaaacga tcaaaatcca atttttttcc tcctgctaaa acgacttttt tttctttcaa
    15541 tcctattact gtcttttctg tgtaaacatg gattcgatag cggtcagtca gttcttcttt
    15601 cgtgatccaa gaaagttgtt tttcactggt aacctttcct tttaatatca aattgaaccc
    15661 gctgggaatc catcctaact tcgattgctt ttcaatcaaa tatatttcac ttgttggcat
    15721 caattggcga agataaatag ccgcatgaat ccctgcaaac gacccgccga caattacaat
    15781 gttcatctgc attcctcttt cttataaggt gttctactgc taacttctca atcagaaaac
    15841 acagtattca taactcgctt tcaaccttat gatacatagc gatgcagcag aatcaaccct
    15901 ctccatcata ccgtgttcta ggtctgcttc tcaataatgt actagtggac ctaattgaag
    15961 agtatcattt ctgaagagat gtttcaaaaa atgtactctt ataaaagaaa aaaactcgcc
    16021 ttttacgtac ctctgtccaa ataggaaaca gaaaagaacg aaaagacgag ttttagcgtg
    16081 tgtaatgtaa tagttagaat agacacatca tttattttta attctttaat gcttcaacaa
    16141 ctgcttcgcc taattgatga gcagattgag gattttgccc tgtaagaagc cgcccatcaa
    16201 tttgaaccac ttctgaaaaa gctgctccag actgatagtt tgctcctttt tctttcaaag
    16261 catcttctgc taagaatggt acttcattcg ttgtaccatt tgcttcctct tcttcattac
    16321 tgaacccagt cactgtacgt ccagctaaaa tagattttcc agactcatct gtaactggca
    16381 acaatcctac aacaccgtga caaacagctg agaccagccc ttcatttcga tagatttgtg
    16441 aagcgacttt cccaagatct attgcttctg gaaaatccca cattgttccg tggccaccag
    16501 cataataaac cgctgcataa tcatttgcat ccacatcatc aggacgtaag gtatttccca
    16561 aggcccgatt tctgaaatct tcatcttcat aataggccca atcgatttca tccatattca
    16621 aaacactttg cggttctaaa ggtacatatc cgcctttagg actgacaaaa tctgcatcga
    16681 tcccattttt tactaataca tcataaaaat gagtcagttc acttaaccac aaacctgtag
    16741 gacgctcgat cttttcgtac ttttctacat ttgttaatac gatcaatact ttcattttcc
    16801 catcctcttt ctataaaaat tgcgttattg ataacgtgct ttcaagaaat tatataatgg
    16861 gcttggcaaa taaagctctt tatatgcttc ttcaaaaggt ttcgttacaa attcatcctc
    16921 acggttttcc aataatttct ctgcccagtc aggatcgacg atcatttgct gtccaattgc
    16981 tgcaagttct gcatatttca atacctgctc tacatcttcg cgattgcgga tcccaccgac
    17041 accaactaaa gggatctttc cttgaatcgt gttatggacc acttcaagaa tcgatttatc
    17101 gttgtattta tcagaacgag ctattcgatc atacatattt aatgaaacat gcagataatc
    17161 tagttttgac tcacgtaatc gttcaagaag ccagatcgtg tcttcaaaac ggatacctgg
    17221 tgtttcatat tcttctggtg agaaacgata ccctacgatg aatggttttt tagcatattg
    17281 ttcaaccgct gagaaaaccg ttgttaataa ttcttcaata aaacgataac gattttccaa
    17341 gctaccgccc cattgatcct ctcgtctatt cgaatgcgga gagaaaaatt gttggatcaa
    17401 atacgtattt gctccatgca actcgattcc atcaaatcct gattcaatcg ctctacgtgt
    17461 ggctttgcca aatgcttgga ttgtttcaac gatttcttct tcactcattg ctcgtggtgt
    17521 ttccgcatca ggacgttttg ctgcaactgc acttgcagat acaggctgtt ctccagataa
    17581 agttgctcga cttgtcattc gtccaccgtg gaaaatctga ataatcgctt ttgcgccttc
    17641 tccttggatt gctttcgcta gctcacttaa tcgaggaatc atttcatcat gagcgatgct
    17701 gagttcacct ggccagcctt ttccgatatc ttggacattt gcagcaccag taataacagc
    17761 accaagacct ttagaacgtt gtttataata agtgatctca tcttcggtga tcactccatt
    17821 gaaaaagctg agttgtgtcg tcataggcga cagcatcaat cggttcttca attgaactcc
    17881 tgaaggtaaa gtaattgatt gatcaaattg tgacattgtc attcctccca taattcgctc
    17941 tgattaacct atcgagaatc atctatcatt aaaccactcg attagttgta catgcaacta
    18001 atttgcttaa aaaattttat tttttttgta acttatctgt tgcatcattc attgtctggg
    18061 ctaattcttt acttgcttca tttcccaaaa ctgctttgta tgactgatga agttcttccc
    18121 agctaccatc gacttcctta gcaaattggc gtccttgttc tgtaagataa actaatgaca
    18181 agcgaccttc gctaactgtt ttgacgtatc ctttatattc taatttttca acaaaacgag
    18241 caatcgttga tggggcggaa tagagcattt gtgcaatatc tttttgtggt attccatctt
    18301 tttcttttaa tatcaataaa ataaaaccat aagttggtgc cagacctgtc cttctaaacg
    18361 cttcatcagc taatttattc aacatgcgat ctagcttttt cacgctaaag aaaagacatt
    18421 catctaacgc tttttcaatt ggcttttcca tttcatcccc tccattggcg tacatatcat
    18481 agtatgattt ttattttttg tcaaaaaaca cggaataatg tccgcctcct ctgtctagat
    18541 ttgaaaaaaa gaagtagcca tcttcaaaga atttcggctt gttttcgaca aaactccttc
    18601 tggaacaccg tgaatcagag gttgtgaaaa agtcgtttag ttttgagtat gaagaaaaat
    18661 tttgtgaaaa tggctttcca catttttgca taaatttggc ttaataccga aaaactggct
    18721 tttgaacacc gtttatatca gaggttgtga aaaagccgtc ctgcatcaag taataagaac
    18781 agccaaacaa aaatagttcc tcatattttg tcgttttttg agctaatgcc gatggtcagc
    18841 tgattgtaca ccgtttattg agaggtcgtg agcctgccgt tgaactccga gtcacaagga
    18901 gcaatttcaa aaaacagctt ctcatgtttt atgactatgg tgacttgtga tcgaggagtt
    18961 ggcaggagaa tactgtttat tcgtaaagaa ggggctgtga caagactttt gtcacagtcc
    19021 cttatttcct tttattatag gatttcgcca tttgattgga tgacttgtct gtaccaatca
    19081 aatgattttt tctttaaccg tttctttgtg ccattccctt cgtcatcttg atcaacatag
    19141 atatatccat aacgttttga catttgtgaa gtagaagcgc tgacgatatc aatgcagccc
    19201 cacgacgtat aaccaaataa atctactcca tcttcgatag cttccttcat ctgctcaata
    19261 tgctgcttca aatagtcaat gcggtaatcg tcttgaacgg ttccatcttc ttctactttg
    19321 tctatagctc cgagtccgtt ttcagctatg aacagtggtt tttgatacct atcatataat
    19381 tgattcaaag caattctcaa acccttagga tcaatctgcc agccccattc acttgatgga
    19441 agatacggat ttttaattcc tccagttatt aagtttcctt ggaccccttc caaattttct
    19501 ggatgagcac tgactgcact agacatgtag tagctaaaag aaacaaaatc aacagggtag
    19561 gctgctaaga tttcttcatc acctacttct atttttaagt tgacaccttt ttcttcaaac
    19621 attcgttgcg tatacgctgg ataatatcct cttgcttgaa catcactaaa gaacaaagaa
    19681 gttctttgct gctcaattgt tttttgaata tcatccggat cacaagaata cggaacaaga
    19741 agctgatagc tgatcataca accgacttgt gaacctttga taatttcatg acaagctttt
    19801 gtagctaaag cacttgctat aaactgatgg tgtgcacctt ggtatttcgc ttgttccaaa
    19861 tggggatggt tttcttcaat tactcctacg ctaacaaaag ggtgatgttt tacacagttg
    19921 atctcattga aagtcatcca atattttact ttatttttat agcgtttgaa tactgtttca
    19981 gcgaaacgaa cgtaaaactg aataagcttt cgattaggcc atccaccata ttctgtaacc
    20041 aaatataacg gcatttcata atgggaaaga gaaacgactg gctcaatccc atattttaga
    20101 cattcgtcaa acacttgatc ataaaaaaat aatcccgctt cattgggtgt ttcttcatca
    20161 ccatgaggaa aaattcttgt ccatgcgata gacatccgat agcatttaaa acccatttca
    20221 gcaaataaac ggatgtcttc tttaaagtga tgatagaaat catttccgtg tcttttagga
    20281 taataatact tgctgttggg atcctgcgcc gcttttactt gctgatgcgt catcatatgc
    20341 cattgatctg tccatttttc tttaggtaaa tcgctgtcga atgtatacac atcagacaca
    20401 gataatcctt tttcatctac atcccatgca ccttcacatt gattggccgc tgtcgctccg
    20461 ccccataaaa atccttctgg aaattttttc atatcgatcc ccctaaactt gctagttttc
    20521 ttgtttgatt atttttctta tgactagtaa tgatattttt gaatctacta ctatttgtaa
    20581 caataaccat tgtggtcgga tcatatccct ctttgattaa ttcttgcaaa tctacttcca
    20641 tcaataaatc gcctttacgt actttttttc catcagatac tgcactttga aagtatttgc
    20701 catttaaatt cactgtatca ataccgatat ggatcagtat ctctgctcct tcttcactga
    20761 ctaccccaat tgcatgtttc gttggaaaaa ctgttgaaac ttccccgttt acaggactgt
    20821 agatttttcc gtccgttgga atgatcgcaa acccttctcc taaagatcca ctagcaaatg
    20881 ttggatcgtt tacttcagat aaatcaatta tttcacctgc tgcaggagta cttaacacta
    20941 tatcctttga ggattcttct tcgatttcat ccttgtagcc gaaaagaaaa gttagcatac
    21001 aggaactgat catcgctaag aaataactag caactagcca aacaaatcct tcaccaatat
    21061 aaatcgggaa agacaaaata cttcttgtag caactgccaa agcaccggct cctgcggcac
    21121 cagctactcc accaaatata gctccacata tacaagcaat gtaaaatggt cgtttatatt
    21181 taagagtcac accataaaca gcaggttcag tgattccaaa taatgctgtg ataaatgcag
    21241 ataaagataa actttttact ttcttatttt tcgttttcaa aaatacccca agtactgctc
    21301 ctgcttgcgc agctacggca gtcatacatg ctggacctag cgtatcacga ccgtagaaag
    21361 aaagattgtt ggtcattact ggtacaagtc cccaatgtaa cccaaaaatc actaacactt
    21421 gccaaaatcc gccgataaca gcaccagata aaacagggct aaagttataa acagctaaat
    21481 atccagctgc aatcagctga cttgcataat ctgcaattgg acctacaact aataaagtaa
    21541 ttgggaccat cacgatgata caaatgaatg gtgtcaataa atttcgaata ctttcgtgca
    21601 agcttttgcg aatcttaggc tcaatataag aaagaaccca tacagctaaa attgctggaa
    21661 taaccgtact tgtatatctt gctagaatca cgggcatccc tagaaatcgc aaggttatgc
    21721 tatcactata tgcactgaca atcgtaggat aaacgagagc tgctgcaata accatagcga
    21781 tgaaacgatc tgtattgaac ttttttgcag cagtatatgc caaaaatata ggcaagaaat
    21841 agaaaaatgc atcagctgct gcattcaaaa tgatataagt tcctgattca gtagttaata
    21901 aattcaaact cgtacataag gcgagtactc ctttgatcaa accacttcct gccaagacgc
    21961 ctaaagtcgg ggcaaaaaca gcggcgatca tgtctataaa agagtttaaa atgccttttt
    22021 tctcagttcc tctctttcca ctagatgcat catcttctag tttcatttgt ttggataata
    22081 cgtcatacac ttcatttact gcatttccta ctacgacttg aaacattcct ccagcttcga
    22141 ctacagtgat cacaccttct agttgctcta ccttctcctt atctgccttc ttattatctt
    22201 ttagggtaaa gcgtaacctt gttgcacaat ggtaaacatt gctgacattt tcttcgcctc
    22261 cgacaaatcg gacaatatct ttcgctaatt gtgaatagtc catttttact tcccccttgt
    22321 taattaatgc cgtagcaatc cttcttacaa gataagtata gttcttagaa aacggtttct
    22381 ccattcaatt agtacccctt ctaatagcga acgaaactgt acaatagtgt gtatggttta
    22441 cttagccgag caaatcactc actgcttgaa agaaaacaag tatatcgttt ctcttatagt
    22501 caaaggagac aaaaaaaaca cgcttgatct tcctttgctg atgtataagt ggtatctcaa
    22561 gctgatactc tgtgattttt gtttctactt ggcttttgat aagacagcac aacaaagtct
    22621 cggcatttaa cggaagcata actaggtcag aactaggaga gattagttgc tgaatggaat
    22681 ctatcttttt atggattttt atttgatttt cttctttgta gtaatgatca agcagttttg
    22741 ttttcttttt ttcttcaact atggtcagct tttcacgcaa aatctgataa atctccgaag
    22801 attcaaactc atctagaaca ctaggtagat aaatcaaatc atcccactca aaaataagct
    22861 cttgctcagt agtcaaaatt gcatcatatt ccgttatttt gtttgattga tttttgaaaa
    22921 gatacactgg ttctatccgg cattctttga tccacatgtt catactttgt tgaatctgtt
    22981 tttttagtgt attcaaaata ctaattggct ggttactaac aatcagtatc tccccttgtt
    23041 ttcgaaaagg attcaacgct tcggcaagat acaaaaccaa ataagcgctt tcagctaaat
    23101 tgggacgtaa ttgaaaatgt attgggaaga aacgccgaat taaataaatt tctaaaggat
    23161 aacgggagat gatcttttgt acaaaatgat tcatggtatt atgtccggca tccatcctcg
    23221 ttttcatttg tttgatatgg gttagcaaaa attctgtttc atggaacaat aacgaaacag
    23281 gaagatctaa aaaacgaata atcgtttgct caaatgctct tagattcttc tctatttctt
    23341 tgtttttttc atcattaatc atcaaataat ttaactctaa tagtcgattt tcaattgctt
    23401 gtttttcttg attggaaaaa gaatagggta gttctgatga taactttttt gtgagggtct
    23461 ctaccatcgg aataaataat gtatacctgt ttgttttttc taaaagttct tctcttcgaa
    23521 gaatggtcaa aacgagatac cgagaaaatc gtatgaagtc ctctcctgaa acaatataat
    23581 tatatgatat aagaacctct tttaatgtct tttggacagt cggcatgata ttggaaaaaa
    23641 ttgtactgat ttgttgaggc aaccttgcgt gttggatcac cgtttctgtt ccaacaagag
    23701 ataagaaaat tctttttgaa ttttcttcag caatgatttt tagtcctttt gtcgacgaaa
    23761 cttccagttc taatttagga ttgcgctcga tccaacgcaa aatcgttttt atttgtaatg
    23821 atacggctgt ttttgaaaga aagaaaatgt ctgctatctt ttgcatactg acataccctt
    23881 tttgaaacaa taagtacgtc acaatagttg aagctcggaa atccatgctg gaatagattt
    23941 tatgtttcgt cacctcttgt atgatttgct gtttagtttc agattcaatt gattctaaaa
    24001 aataacctga acgtttattt gactggataa tgattttctt ctcgtagata gcaagcatat
    24061 ttttgatttc agtttggatc gttttcttgt tggtacctaa agcttcagct aattcttcgc
    24121 ttgttatcca atgattctta tttcttaata ctacaagaat ttggagttgt cgtttattta
    24181 agtccatttg cttacacctc ctctttgtta taaacataca ggaaaaacct ctcaaatgac
    24241 aatagtcact tgcagaggtt taaaaatcat ttgaagaaag atctatattc aaggttttta
    24301 ccattagact ggataatttg tttataatat tcaaaactat cttttttcaa tctcctaaag
    24361 cttccgctcc catcattttg tctatccaca taaataaagc catatcgctt cctcatttcc
    24421 cctgttcctg ccgatacgat atcgattggt ccccaccata agtaaccaat gatttggcaa
    24481 ccatctttta ttgcctcagc aatttcctga agatgtattt ttagatactc acaacgtttt
    24541 ggatcgtgga ttcgattcaa ttcatctggt gcttcatcta gtccaatgcc attttcaact
    24601 atgaataatg gtaattcata acggtctgtc aattcattca tgacataacg aaaccctaga
    24661 ggatcaattg gccaactcca aggttcggga gatttttcct tcaaaaaggg attatcgaaa
    24721 ccatttgctc cgccagtatc tacgcgcatt tctgcttctt ttttatagac agcgctacga
    24781 taataactaa atgcaatata atctaccgtg ttagcttgca agtactgttc gtctccatct
    24841 aacattactg gtgcacagtc ttcttccttc catatcctgt aaatataagc tggataatgc
    24901 cctcggcaca tgacatcgga gaaaaaccat gaattgcgtt taaattgcag tgaaccaaat
    24961 acattctctg gatcacagtc atatgcataa gtcgctaatg cagaaaagct acacataata
    25021 cccatcttag catttggatc gattttcttt aataattgag tagcacgggc gttcgctaga
    25081 aacatatgat gacaggcttg ataaatctct ttttttgtta gtgtagcaat gggttcaagt
    25141 ttatctctag cagggaatgt aaccaatcca gcagctgcat aaggcatacg aaaagcatta
    25201 ttgatctcat taaaggtcat ccaatatttc actttccctt ggtagcgttc aaagacggtc
    25261 tgcgcataaa attcaaaaaa gtcgattaat tttcgatttt gccacccccc atactctcct
    25321 accaatgcta agggggtttc ataatgacta agagtaatta ctggctcgat atcatatcga
    25381 tttaatgtat tgattagtct gtcataaaat acgagaccag cctcatttgg cttctgttcg
    25441 tctcctcttg gaaatattcg tgcccatgag atgcttgttc gaaaagcttt taaacctaac
    25501 tccttcagta aacgaatatc ctcttcaaat cgatgataaa aatcaatcgc ttcatgagaa
    25561 agataatgga gtgattcgtc taaagcaatc tcccaaatct ttttttcttc attccactga
    25621 atactaggat gctggttcat gatcccgata agcgtatctg tcacattagg taatttgccg
    25681 tcttccaacc atgcaccttc acattgatta gcagctactg ctccgcccca taaaaaattt
    25741 tctggaaata tttcctgatc cataactatc tctcctcgct atcaacaatt tcttacttga
    25801 agtatagctc gattcatcaa aacaagatat tctaactatt tcggcagaag agaagaaaaa
    25861 aattgaatgc tactcttcat ttttaatatt tatatataac aaatacataa gacgatcccc
    25921 gaaagaaaac actcgttaac catctaagca aacgatttat tttacttcca acacaaatgc
    25981 ttcataaggt cgtaaatcaa ctttgccgtc tggtaactgt ttcagaggat aatttgacac
    26041 aataaccctt aaagcttcac gtggaacagt gaacgtctgc ttttcactgg acatattaca
    26101 aataacgagc cattgctggt cttcatatat gcgagaatag gcaaagacat gctcaggcgt
    26161 cttttccact aattcatagt ctccccagac gatgatgtca tgttttctac gcaaatcgat
    26221 caatttcttg tacatataaa aaacagaatc ttgatctgct aacgcttctt ctgcattgat
    26281 ttctttataa tttggattga ctcgaagcca tggaattcca gtagtaaacc cagcatgttc
    26341 acgggcgttc cattgcatag gcgtcctagc attgtctctt cctttcgcat tgattgattc
    26401 caagatttcc tcttttgtaa atccagaaga aatcctttcg ttatacatat tgattgtctc
    26461 aatatctttt gcttccgcga tatcatcgat tgggcaattc gtcatgccta actcttctcc
    26521 ttgatagata taaggggtgc ctttcatcat atggagcaag atagcaaaca tcttggcact
    26581 ttcttttcga tacacatcgt catttcccca acgagaaaca attcgcggaa gatcgtgatt
    26641 attccaaaat aaactattcc atccttcatc acctaaagat gtttgccatt tggataatac
    26701 ttttttcagt tcagagatac tcaagggttt caaatcccat ttgtctttgc cttcttgctg
    26761 atctaatcct atgtgttcga attggaagac catggataat tcatttcttt taggattgga
    26821 ataaagttta gcgatttcgg tagttgctcc ccatgtttcg ccaacagtca tcagattttt
    26881 gtcaccaaaa gttgcttgat tcatttcctg aagatactca tgcagtttag gtccattact
    26941 tgtgatttct ttatccggtt ctttgccaat catatcgatc acgtccatac gaaagccacc
    27001 aataccttta tcgatccaga aattcatcat ttgataaatc tcttggcgaa cctgtttatt
    27061 tttccaattc aaatcaggtt gacgcttgct atacaagtgc agaaaatatt gccccgtttt
    27121 ttcatccaac tgccaagcag aaccactgaa tattgagcgc aatctattag gctcttttcc
    27181 attgaccgga tcacgccaaa tataatagtc ccgataagga ttttctttat ttttacgggc
    27241 ctcaatgaac cacggatgtt catcagaagt atgattcacc accagatcca tgatgatacg
    27301 gatatttctt ttctttgctt cttcaatcag ttcttccatt tcttccatcg taccgaattc
    27361 tgccatgatt gcttgataat tactgatatc atacccattg tcatcattcg gagactcgta
    27421 tactggagaa agccaaattg catcaatacc taaatacgcc agataatcca acctggaaat
    27481 aatcccccgc aaatctccaa taccatcttg atttgaatct tgaaaacttc ttggatagat
    27541 ttgataaaca actgaatggt gccaccattt ttgttccatg attttcctcc tataaatgga
    27601 tgtttctatt ttttgcttaa tcatttattt tagtctctct cactaaaaat tgtaacgctt
    27661 tcaaatagct attgcacctt gttatcggta acagaataaa acaaaaaaac agctgaggaa
    27721 attccctcaa ctgttttttg aaaggattga tttattcagg gaagaatgct gatttcacaa
    27781 cttctttata aacggtgttc actcagctgt ccctcggtat tagctcaaaa aacggcaaaa
    27841 tataagaaac ta
//
LOCUS       DAKBVG010000010        26720 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000010 DAKBVG010000000
VERSION     DAKBVG010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 26720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.010"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(<1..1907)
                     /locus_tag="OUD09_000370"
     CDS             complement(<1..1907)
                     /locus_tag="OUD09_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCT9691676.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLR"
     gene            complement(1925..2839)
                     /gene="pfkB"
                     /locus_tag="OUD09_000371"
     CDS             complement(1925..2839)
                     /gene="pfkB"
                     /locus_tag="OUD09_000371"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCT9691677.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(2836..3588)
                     /locus_tag="OUD09_000372"
     CDS             complement(2836..3588)
                     /locus_tag="OUD09_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCT9691678.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(3748..3957)
                     /locus_tag="OUD09_000373"
     CDS             complement(3748..3957)
                     /locus_tag="OUD09_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9691679.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(3968..5296)
                     /locus_tag="OUD09_000374"
     CDS             complement(3968..5296)
                     /locus_tag="OUD09_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9691680.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            5432..5587
                     /locus_tag="OUD09_000375"
     CDS             5432..5587
                     /locus_tag="OUD09_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9691681.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(5661..6248)
                     /locus_tag="OUD09_000376"
     CDS             complement(5661..6248)
                     /locus_tag="OUD09_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCT9691682.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(6331..7581)
                     /gene="clpX"
                     /locus_tag="OUD09_000377"
     CDS             complement(6331..7581)
                     /gene="clpX"
                     /locus_tag="OUD09_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HCT9691683.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(7828..9117)
                     /locus_tag="OUD09_000378"
     CDS             complement(7828..9117)
                     /locus_tag="OUD09_000378"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCT9691684.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(9271..10203)
                     /locus_tag="OUD09_000379"
     CDS             complement(9271..10203)
                     /locus_tag="OUD09_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9691685.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(10360..11019)
                     /locus_tag="OUD09_000380"
     CDS             complement(10360..11019)
                     /locus_tag="OUD09_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HCT9691686.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(11022..12047)
                     /gene="sppA"
                     /locus_tag="OUD09_000381"
     CDS             complement(11022..12047)
                     /gene="sppA"
                     /locus_tag="OUD09_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCT9691687.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEKGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(12060..12350)
                     /locus_tag="OUD09_000382"
     CDS             complement(12060..12350)
                     /locus_tag="OUD09_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9691688.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            12759..14564
                     /gene="glmS"
                     /locus_tag="OUD09_000383"
     CDS             12759..14564
                     /gene="glmS"
                     /locus_tag="OUD09_000383"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCT9691689.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINCLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(14636..15991)
                     /locus_tag="OUD09_000384"
     CDS             complement(14636..15991)
                     /locus_tag="OUD09_000384"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCT9691690.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(16015..17184)
                     /locus_tag="OUD09_000385"
     CDS             complement(16015..17184)
                     /locus_tag="OUD09_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9691691.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(17181..18068)
                     /locus_tag="OUD09_000386"
     CDS             complement(17181..18068)
                     /locus_tag="OUD09_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HCT9691692.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(18257..21949)
                     /gene="nifJ"
                     /locus_tag="OUD09_000387"
     CDS             complement(18257..21949)
                     /gene="nifJ"
                     /locus_tag="OUD09_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCT9691693.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            22148..22669
                     /locus_tag="OUD09_000388"
     CDS             22148..22669
                     /locus_tag="OUD09_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HCT9691694.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(23368..23925)
                     /locus_tag="OUD09_000389"
     CDS             complement(23368..23925)
                     /locus_tag="OUD09_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9691695.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(24104..24715)
                     /locus_tag="OUD09_000390"
     CDS             complement(24104..24715)
                     /locus_tag="OUD09_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9691696.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(24828..25085)
                     /locus_tag="OUD09_000391"
     CDS             complement(24828..25085)
                     /locus_tag="OUD09_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9691697.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(25682..25960)
                     /locus_tag="OUD09_000392"
     CDS             complement(25682..25960)
                     /locus_tag="OUD09_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9691698.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(25994..26245)
                     /locus_tag="OUD09_000393"
     CDS             complement(25994..26245)
                     /locus_tag="OUD09_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9691699.1"
                     /translation="MKEKPVNKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         7551 a         5750 c         4578 g         8841 t
ORIGIN      
        1 tttctcaata aaccataaat cacaccagat acgactgctc cgatagcaat aaacaccaaa
       61 tacatgaccg ggtgactcag taagaataca acgaaaatcc cgccgtgagg agccagtaat
      121 ttgattccga aagctccgac taatccgcca gttaaagcag aaccaataat aaaacttgga
      181 atggcacgaa tcgggtcagc tgctgcaaat ggaatcgatc cttccgtgac aaacgatagt
      241 cccatcacga tgttcgtcaa accagcatct tgatctgttt tgctgaattt attcttaaac
      301 aaacgtgtag caacgaaaat tgccaatgga ggtaccattc ctccagccat aacagaagcc
      361 atcacgacac ttccgccagt tgcaacagtc gtagctaaag ttgctgtacc aaacacataa
      421 gcggctttgt tgattggacc gcctaaatct gccgccatca ttccagctaa taatgcacca
      481 agtaaagcag cattcgaacc actcatactt gtcaagaaac cgttcaatgc atcattgatt
      541 gctttcatag ggacattgac cagcaacatc aagaaaccag taatcagtaa gccaaacact
      601 ggataaaaga ggatcgtttt aattccatcc aatgattttg gtaagttctt gaataattta
      661 cgtaaaaata cgatgacata accagctaag aatccaccaa tcagtgcacc taagaatcct
      721 gcaccgcctg tattagctaa tgcaccagca gcaaatccaa cgatcaaacc agggcggtca
      781 ccgatacttg aagcaataaa tcctgctaat accggcagca tgaaaccaaa tgcagcttgt
      841 ccgatttggt tgaaccagct agcagcttga ttgtaattcc ctagatttga taattgatca
      901 tgtggcacgc caataaattg gtcgatcatg aaagaaagcg caatagcgat tcctccacca
      961 ataacgaaag gtaacatgtg tgatacgccg ttcatcaaat ctttgtaaat tctttgtccg
     1021 atagtaccat ccgcatttcc ttcttctgaa ctgctttctt ttccatcacc atggaatact
     1081 ggtgcagtac cactaagggc tagattgatc aactcttctg gtttacggat accatcactg
     1141 acaggacgat tgatcagttc ttttccgtca aagcgattca tctctacttt tttgtctgct
     1201 gcaacgatta caccttctgc acgggcgatg tcttcagcag tcaatcggtt tttgattcct
     1261 tctgaaccgt tcgtttcgac tttgatctcc acacccattt ctttcgcttt tttcttcaaa
     1321 gcatcttctg ccatgtaagt gtgagcgatc cctgttggac aagctgtaac ggcaacaacg
     1381 aattttcttt ctggatgagc agaagcttct gcagcttgtg tttcttgttc tgctttttct
     1441 tctgcttctt tttgctcttc agctgtttgg aacaagtctt ggacttcttc tggtgtattt
     1501 gcttctttta attttcctac gaaatctgca tcaatcagca atcgagatag tgctgctaat
     1561 gcttgcaaat gtgtgtcgtt tgcgccttct ggagctgcga tcatgaaaaa cagataagtt
     1621 ggctgtccgt ctaaggcttc ataatctact cctttttggc tttttgcgaa taaaactgtt
     1681 gcttctttaa ctgcactgtt tttcgcgtga ggcatggcga ttccatcacc aagacctgta
     1741 gaagtttgtg cttcgcgggc tagaattcct tctttatatg tttcgatatc ggaaatccgg
     1801 ccggcatcaa acattttttg gaccatttca tcgattgcgc cttttttatc agtcgcttgc
     1861 aaatccatga tcatcgcttc tttgaccatt aaatccttga tattcatgat tatcctcctt
     1921 gaaactaatc aatttttgtg atttcaactt ctggcagcaa ctcatcaatc aattcgcgag
     1981 ctgccaagtc atcagaaaaa gctgttgcgc ttccgcaagc aactccccat ttaaaggctt
     2041 cgattggatc tttcgtacgg ctgtaagccc caataaagcc agcaatcatt gaatcccctg
     2101 cacctactga atttttcaat gggcgaacta atacattcgt tttataagtt ccttgttccg
     2161 tgaagagcaa agcaccgtct ccagccattg agatcagcac attctgtgcc ccttcagcta
     2221 gtaatttttt cccatacgga atgatttctt ctacagattg gaaggttgtg ttgtaaagct
     2281 cagctaattc gtgattgttt ggtttgatca acaacggttt cttaacaagt gcatttttta
     2341 ggtcatcacc tgttgtatca atcacgaaat ctgcaccttt ttctcgaatg atatcaatca
     2401 gttcttgata aaatcctttt cgcaggctag ctggcgcgct tcctgacaat acgacgatat
     2461 ctccttgtcc tactgttcct aatgcttgct tcaactcatc gatttcttca tctgaaatag
     2521 atgggcctaa gccattgatc tcagtttcag tgtctgattt tagtttgata ttgattcttg
     2581 tatcttcttt tacggttgtg aacaaagact ggatttgttc tttttcaagc cacttcgaaa
     2641 taaattcgcc agtgaaccct cctaaaaacc ctagtgctgt agattgagaa tcgatccgtt
     2701 tcaaaatacg ggaaacattg atcccttttc ctccaggtaa cttgaagtct tccttcattc
     2761 ggttcaaatc gcctagtttt aacccatcta cgcgtacgat ataatcaatt gatggattaa
     2821 gtgttactgt gtaaatcatt gtattgcctc cttaattgtt gtcttttcac ggaattcatt
     2881 aacgaattct aatggacaac gctcggttat gatcgtggct gcttccaaag gtgcaacaga
     2941 ggtgaaggac aactggttga atttggtatg atcaatcaat acaaatgtct cttcggaatt
     3001 tttcatggct aaacgtttaa cagctgcctc ttctggatcc ggagtcgtga atccttgttc
     3061 aagatgggca ccgttcattc ccataaatgc tttattaaaa tgaaaatttt ttagttgttc
     3121 tatcgcatta gatcctaaaa cagcattagt tgccagcttt atcgttccac ctaaaataat
     3181 ggtagcaagc tccaaatcaa ctaaatttgc cgcatgttga acggaatttg tcactactgt
     3241 gatctgtttt ccttgcagaa aaggaatcat ttctaacgta gtagagccag cgtctaaata
     3301 aatgacatct tcgtcattaa tggtttcagc tgccgatttg gcgatttgct gtttttcgtg
     3361 aacgtttttg agtgattttt cactcatatt ctgctcaaaa ttcaataatt gctctttctt
     3421 agctccgcca tgaactcttt ctagaaaccc actattctcc agctcttgta aatcacgtct
     3481 tatcgttgat tcagaagcat tcaaaaggtt tacaagatct tgtgatttca cgattttctg
     3541 ctgttcaagc aagtctagga ttttttgtct gcgttcctct gtaagcattt tcacgatcac
     3601 ctcaatacaa ataatagcat attttttttc tactagcaat ataaatcagt caaaaacgtt
     3661 cataaaagtt ataaatcagt caaaaaaaaa ataaaaaacc agtcagaaag actggttttt
     3721 tatttttttg tctatcatca agccgtttta tcgtttttta ttttcgcttc ttgttttttt
     3781 catataaaca gaagcaggtt ttctttcctt atgaagaaga taataataaa gtcccatccc
     3841 gccaaacatg accaaaaaac cgatgactga tcgaatagga aaattaggaa caaaaaagag
     3901 acagacggat aataatgatg ccggaaccat tattcgataa tagagttgtt tatccatttt
     3961 cttcacctca tattttttgt aaagtaatca attgttctaa ttctcggtaa tctaaaaatt
     4021 gcacacgcag atgctgctca cctttcttga catcgaattg gatacttcgc cgctttttct
     4081 ttattcgttt gaaggtaaca tcagagagtc tatgtttgat ttcctttaac ggtaattctg
     4141 acagggattc tgaaggaaca tagattctta aggataaaag atactcttga tatttttcat
     4201 ctgttacctc tacttctaga tattccctaa tataataaca ataagcttcg attccattat
     4261 ccaaagcaac agtcggatcg ataactttat agcacatcat gcgtgatcga ttctcgtcaa
     4321 cccaaggaaa atacgcctga gcatttagtg ttccgcgaac acgcctttct ttataatctc
     4381 cttgttggat atcataactt tttgttacaa aaaatgaagc attcttttta ggaacaggtt
     4441 gcatttgtgc attctgatac tggacagatt ggatcgtatg gtcagtgtaa atgtattcaa
     4501 cgaatggcgg tttcaataaa ccgacaccat tgtcccaagc aagatgatca aacccaagct
     4561 ggtccctaaa ataggattga aacatttgat tgtttggctt ttgccagtta aaggggtctc
     4621 gataaagctg gatacatatg acttcgtttt ctaattcctt cactttttct acttgttgga
     4681 tgtcgagaaa aatcggttta gggatgattt gatgataata gcgactataa tacatttccc
     4741 aacacgaagt gaaacataaa ccttggtaaa aaagatcgta tcctggaaat tggttgcaat
     4801 cgactactac ttccccgttt tgttgtttca ttttaggtaa atgttcgatt tcatcctttt
     4861 tctcaaacaa tagccgttga gagatctgtt ttgtattgtg atagataaac tcgctataag
     4921 ctcgcatata ggcgaacacc ccatgagcaa tcattttttt ctgaatgaag tctcgaatca
     4981 aaaattgctt ttctttgaaa acagagatag acaatacatt ttttatcaat aattgattat
     5041 cttccaagcg aaaactacat tcgttctcgt catttcctac aactaaccaa ctttcgtctc
     5101 ttaccgatgc tagcattatt ttttcttgaa gctttcgaag atcatacgga tccgcttgtt
     5161 gtgtgaaaga gcggataaat gttggctgga tcaacagacg tgtcaaaata gcccaatcgc
     5221 ttatttcctg ttttttttct tcttcattta agtctgtgag ctggatagtc atcaataatt
     5281 tatcttcgcc aatcattttc tcacctcttt caaatctttt tataatttaa ttataaccaa
     5341 tgataaagaa aaactaaaac tttatgtctt agaaaccaat ttggacgatt acatgataga
     5401 ctaagtaaaa agaaacaaag aggtgtttac aatggggttg aaatttgaac gtacagataa
     5461 tttagataaa cttttcgaat catttgctgt cgatccggaa aaaacagttg caccaaaaga
     5521 agagatcgat cgatttctaa aaccacagcc acaaaatgaa aagaaaaaag acaagaaaca
     5581 aaagtaagtt aagctcaaaa aaagacttac cagccaaact tttatgaaag agtttgactg
     5641 gtaagtcttt tttattcttt ttatgattca attgcttgtt cgattgctgc ccacgcttct
     5701 tcttttccct gttttgtaac ggaagaaaag agaataaacg tgtcattgcg atcaaaatcc
     5761 aacgcttttt tgatttcgct ctcatgcttg ttccatttac cacgagggat cttatctgcc
     5821 ttagtggcaa caacaattac agggatatca taatacttca aaaattgata catctggata
     5881 tcttcttgag aaggcttatg acgcaaatct actaaagaaa cgacagcacg tagctggtct
     5941 ctagtcgtta aatatgtctc gatcatcttt ccccattttg ctcgctcagt tttggatact
     6001 tttgcatacc catatcccgg aacatcgaca aaatgtaatg cgttctcgat caaataaaaa
     6061 ttcaatgtct gggtctttcc tggcttaccg gaagtacggg ctaaattctt tcgatcaatc
     6121 aatgtgttga taaaagaaga tttccctaca tttgatcgtc ctgccagagc tatttctggc
     6181 aattgtgtat ctggatattg ttttggtgcc actgcactta tgataatttc agcttgatgg
     6241 actttcatgg tctctctcct taacaaggtg atgaataaag caagcggctg tcgcaaaagc
     6301 caaaatgact tttacggcag cctcttttca tcaacccgct tttttctctg aatctttata
     6361 gattactgtt ggctcaccag acaattcagc tgcttctttt gtgataatca ctttttcgat
     6421 gttttcattt gaagggatat caaacatcac atccatcatg atttcttcaa tgatggaacg
     6481 aagtcctcga gcaccggtat tgcgttcgat tgcttttttc gcaatggctc tcaacgcttc
     6541 aggttcgaat tcaagttcgg tattatctag agaaagaagt ttttgatatt gtttgactag
     6601 cgcatttttc ggctcagtca agatccgtac cagatcatct gttgtcaatt tctctaaagc
     6661 tgccataaca ggtaatcgtc cgataaactc agggatcaat ccgaatttta gtaaatcttc
     6721 aggaatgata tgctgcatga cactttcgtc ttcagaaagt ttttggtttt gtgtaccaaa
     6781 tccaatcgtc ttttcgccca tacgattctt cacaatcgtt tcgatgccat caaatgcacc
     6841 acctacgatg aacaatatgt tcgtcgtatc aatctgaata aattcttgat gtggatgttt
     6901 acgtccgccc tgaggtggca cacttgcaac agttccttct agaattttca gcaaggcttg
     6961 ttggacacct tcgccggaaa cgtcacgagt aatagataca ttttcgcttt tacgcgcaat
     7021 tttatcgatc tcatcaatat agatgatccc tttttctgca agttccacat tgtaatcagc
     7081 agattgaagc aatttaagca aaatgttttc tacatcttcc cccacataac cagcttctgt
     7141 caaacttgtc gcatctgcga ttgcaaaagg tacattcaat gtttttgcta atgtttgtgc
     7201 taaaaatgtt ttaccagacc ctgtaggtcc gatcaagcag atattgcttt tttgcagttc
     7261 aacatcatct gtgttttctt gtgcttgctg attgacacgt ttatagtgat tgtatacggc
     7321 aacagctaac gagcgttttg cgcgatcttg tccaatcacg tagttattca atacttcaag
     7381 aatttctagt ggtttaggga cttcagtgaa ttcgcgtaca gcttcttcat aaaattcttc
     7441 atcaatgatt tctttacata ggtcgataca ttcattacag atgtagaccc ctggaccagc
     7501 tacgatcttt ttcacttctt cttgggtttt cccgcaaaaa gaacagcgta cggtctcatt
     7561 gctacttgga ttgtcataca tggtcttcac cccttaactt gcaaaaagta tgcaagaaac
     7621 tatcaaagac tttacagtct tttactatca taacatagtt tagtaaaaag aaaaagtgtt
     7681 taacaaagga tcaactgacc tataccattg cattttttac tcttaacaaa aaataaggaa
     7741 ctgtgacaaa atcgtgtcac agtcccttat ttcgaataaa caattttcta gaagtagctt
     7801 cttcagaaat gatttattac ttgatttcta ttctttcgct tctgtcgctg tacctgtgat
     7861 caattcgatt gcttttttca ttgtcacatc atgttccagc atatcttctg tcaacacgcg
     7921 tttgatttgt tcgattggca tgttgtatgt ttctgataat tcttggattt ctgcattgat
     7981 ttcttcttct gttgcttgaa gattttcagc agctgcaatt gcttcgatca caagatttgt
     8041 tcttgtgcgc atttctgctt caccttcgaa ttgtttatgc aagtcttctt ctgtagaacc
     8101 agttaattga tagtacattt caggagagat accttgacgt tgcatgttgt tcaagaattc
     8161 gtccattgaa cgatgtactt catcatgaac catcacgtgt ggcaattcaa cgatttctgc
     8221 gttttcaaca gccatacgga tcgctgcttc atcttttgca tcgtccgctg ctttttcttt
     8281 tgtttctact aattctttgc gatatttttc tttcaattcg tctaatgttg atacttcgtc
     8341 atcaacatct tttgcgaatt cgtcatctaa ttcaggcact tcttttgctt ttacttcatg
     8401 gattgtcact ttgaagactg cttcttttcc agcaagatct tctgcttgat aatcttctgg
     8461 gaaagttaca tttacatcta gactttcacc tgatttatga ccaactaatt gatcttcaaa
     8521 acctggaatg aatgagcctg aacctaattc aagtgagtaa ttttcgcctt ttccgccatc
     8581 aaacgcttcg ccatctacaa agccttcgaa gtcgattact actgtatcgc cattttcagc
     8641 tgcagcatct tctttgataa ctaattcagc ttgtgcttct tgttcacgtt tgatacgtgc
     8701 atccacgtca gcatctgtta cttcacggtc ttgtttttca acaactagat ctttatattg
     8761 tcctaatttt acttctggtt tcactgttac ttcagcagtg attacccagt cttgaccttt
     8821 ttccatgctt tcaacatcga tttttggttg agaaacagga tcgatgccag cttctttgac
     8881 agcagcttca taagcttctg gtaatacagc attcaatgca tcttcatata aagcttcttc
     8941 accgtacata cggttgaata cttgacgaga aactttccct ttacggaatc caggtacgtt
     9001 caagcttttt ttcactttat taaacgcagt tgttaatcct ttttggattt cgtcttgcgc
     9061 aatcgtaaat tttaaaacac catcattcgt gccttttttt tcaaatgttg cagtcattta
     9121 attccctccg agtttagaaa ttcattttat acatcaaaaa cgtttcatgc atacctttaa
     9181 atagtacact aaccctttca aattgtaaac atattgaatg gatagaatcc ttgtttttca
     9241 taacattttt atctttttca ctgataaaat ctaatggaag aagatcattt gctgcaaaat
     9301 tttttctctt ttttttaaaa aatcttgttt ttcatcttct gtaccttcat acatcgtttt
     9361 tcctgtacac gcctctacag tcagttgaaa ccagcgcttc gggtcttcga tgtatgtatc
     9421 atagacagga taaacaattg cagattgcaa cgtgaattct tcttttaaca cggaactaag
     9481 cagcgcatct tcattcccgt aacgatctga aaatacacat aattgctggt aacttgcctg
     9541 ctgctgcggc tttggcagat cttttggtat gactttgact aacttttctt caatcgtcaa
     9601 atagaaaaca ggatggcatt cgttcaattg cctaagtgtc tccagtaatt ttgcccttgc
     9661 taaaatatgg acctctggcg atacaataag tttactcgct gtttggataa actcatgata
     9721 aggaagctgt tcgatctcat caatagacgc aagctgacgt gcaggttcca acgaaggaag
     9781 ttcctccgat ttattgtgga gtgccttgat cttctgcctc tccatttgtc cggataattc
     9841 ttctgcaaat tggatttttt caatgacccg ctgctcgaaa gcttcataaa aatctgtact
     9901 tgctatcaat tttctagcat ataaaaaatc ttgtgtactg attaacaagt caaaataaaa
     9961 ttcagcaaac tcctcatttt gaagatactg tttttcatgc aagagtgctt gcttcaatgc
    10021 ttcttttttc tcgtccaact cgtaaagaca aaaaacaagc aggctattag cctcaaagct
    10081 ttctttcaac tcgtaagctc gttgaaataa ttcttttgcc tgctgatatt gctggtttgc
    10141 tagtgcttgt tgtgccactc gcattaaatt ctcatatttt tctggaaaat caatcggctt
    10201 cacgcagtgt cctcctcttg ctcatattca gctcatgttc aatagtagca aaacaaaaaa
    10261 agcatgacaa ttaaaaataa ctgaagcacc ctctaaaagc tagattttag atctgacttt
    10321 tagaaggtgc tttcaactgt tactgctttt tattgctcat cagctgttga cttcctggtt
    10381 gaacgctttg atcagattga tcgtaacaac acttgtatta gtgaaaaaat cgcccagatg
    10441 ttgctttttc tgattaaaga ctgctggaag ataccctaac atcagcaaag gaaattttag
    10501 aatgaaccgg caagcacctt ctctaactaa aacggttgac cagctaagtt cttcttcatc
    10561 aaagctgatg actcgaatac caaagatcat tttcccaatc gtttggccat gattgaattt
    10621 cgtcaataaa ataaaatacg ctagataaat aagtaaggct gtgaatccgt agagactcag
    10681 ataagaatct gaaaaattaa gccaacctag atttttggca ataccaagtg ttaaacgcgt
    10741 aatagctccg atacacaaaa gatcgaccaa gaaggcaaac attcggataa aaaaacctgc
    10801 atagaaatac ttaggaaaat catttgcttt tggcggttca tttcttgtgt ccgtatactt
    10861 tttccattta cgttgtttct ttttgatttc ttcgggcgtc aaaggctctc tttcaaaaga
    10921 gctcagtacc ttttgggaaa agttggagtc aggtcctaca gcaggctgct gttgcttatc
    10981 tttttcttgt tccttatttt catttgaaag ctctgacatg cttactcacc tccgtaataa
    11041 tacattgctt ttggtgattc cgctgtccct agattctcaa gaacggataa gatctgacta
    11101 gtctcagaag gcttcaatcc ctggaattct gctagtttac ttcctaacca tgtactagca
    11161 aacccagatg gcgtggaaga atattctact agttccgcat cttcaagctg ttgttccttt
    11221 cgtatagctt ttaaagcatc ttcaggaaat cccaattcat cgactaaacc tacttctttt
    11281 gcttggacac catcataaat ccttccatca gcaatttttt tgacttcttc ttcagattta
    11341 ttccgtccct cgctgacgat tgctacaaaa cgactatatg cgctgtcgat atatgcttga
    11401 agcaccgctt cttcttcttt tgtctctggt cgcgttcctg aacccatatc tttcaacgct
    11461 ccgcttttaa ctgttgtatc ttccacaccc agtttttcta gaagtcctga ataattcaat
    11521 cctgacatga tcacaccaat tgagccggtc actgtttctt ctgttgcaaa gattttatct
    11581 gcttgagcag aaatatagta gccaccgcta gctgccatgt ttttcatgct gacatacatt
    11641 gggatatcat gttctttacg aatcgtatcc aatttcttag cgatttccgc actttcgtag
    11701 acaccgccac caggagaatt tacttctaaa aagatacctt tgactgttgg atcttcttca
    11761 atcgccttca gttgttccat aaaattttta tgattgtaac cttcagaagc aaataaacct
    11821 gaagaccctc ccgcagcaat cgttccatct actgataatt ttacgatttt ttgcgtactg
    11881 tctccctttt ccaacacatt tggagctagt tcttcatccc catataacca actgttcaat
    11941 ccctcaattt gatcgttttc ctgaggctta gtcaaactag ctgaaactaa cgaaaccaca
    12001 aataatcctg cagcaatcag aactgctgcc caacgtcttt tattcattct ttctcctttc
    12061 taatacaagt tttttatgac gatcacagtt tctgactgct cagaagtgtc gaaccgctct
    12121 gctttttctt cccgcgcata cccggaaaac gactcttttg ttaattcatc ttctaccgta
    12181 aactcacgag cttgagcaac ttccaaggca tcaggtgcga tttcttggat gctatcccag
    12241 ccagtatcta aaaatatttt cgtatcatct gatacaccag ctgatgcttc gaatttggac
    12301 aattgttttt ttaacattcc cagtgtcatg atctcaattt ttgaatccat ttttactcct
    12361 cctctatccg attactttct attgtaccga actcctgaaa aagatgatag ataaaaaccc
    12421 attatttttt gacataaaaa ggacatttga caaagaattt gatatatacc aatttatttt
    12481 ttattctttt tttgttgaca agaacattcg caaaaggtta tgattttaaa tgtttccaga
    12541 taaaaaacaa aaaggaggat agttatacct tttaggatag cgccaggtct gtgtaaacag
    12601 atgacgagga gagggtttat cgaaagattc ggcggatggc cctagggact gcactctaca
    12661 gaaaatccac aaaaaatgtc tgcaaagata aaacaaaagg attttcctgc agctggaagc
    12721 attcatacga attcattata gaaagtagga tgttttttat gtgcggaatt gttggaatga
    12781 ttggattaaa aaatgtaaca ccaggattga ttgacggtct agaaaaatta gaataccgtg
    12841 gttatgattc agctggtatc tttgtctcag atggcacaac tgattatctg gtcaaagcac
    12901 aaggcagaat tcaaaatttg aaaaacaaaa taaatattga tactacagga aatataggta
    12961 ttggtcatac acgatgggca actcacgggc aaccttctga agaaaacgcc catccacata
    13021 cttcccaaag tggacgcttc gtgctcgttc acaacggcgt aatcgaaaac tttgaagaac
    13081 ttaaaattgc ttatttagca aatgaccact ttattggtga gaccgatact gagattatcg
    13141 ctcatctaat tgaaactttc gcgaaggata ctacaacgca agaggcgttt ttaaaagctt
    13201 tgcgggtcat caaaggttct tacgctttcg ctttgattga tcggacagca cctgatgtta
    13261 tttacgtcgc taaaaataaa agtccactat taatcggact cggcgatgga ttcaatgtga
    13321 ttgccagcga tgcaatggct atgcttgctc atacgaagga attcgttgag attgaagatg
    13381 aagagatggt taccgtcaca tcagaaaaag ttattattca aaattttgca ggtgacatcg
    13441 tagaacgttc ttcattcgaa gcacaagtag atgcttcaga tatcgagaaa ggtacctatc
    13501 ctttttatat gctgaaagaa atcgacgaac agcctattat catgcgtcgt atcgctcaaa
    13561 aatacgtaac agaagacaat cgtgtctctc ttgatcaaaa gctggtcgat acattgtctg
    13621 atagcgatcg aatctatatc gttgcttgcg gaactagtta ccatgcgggg ttagcaggta
    13681 aacagacttt ggaaaaattg acacagattc ctgttgaggt tcatttagcc agtgaatttg
    13741 gctataacac acctcttctt tcaaaaaaac cattctttat tttcttgagt caaagtggcg
    13801 aaacagcaga cagccgccaa gtgctggtaa aaatcaactg tctcggctac ccttcactta
    13861 cgattacaaa tgttgctggt tctactcttt cgcgggaagc atcctttaca ctgctattgc
    13921 atgctggacc cgaaatcgca gtagcatcaa cgaaagctta tactgcacaa atagctgtac
    13981 taactttatt agccaaagct atcggtgata aaaaagaatt tgaaacttct cttgtattcg
    14041 atatcactca tgaattgagt ttagttgcta atgcgatgga atcggtcatc gcccaaaaag
    14101 attatttaga agagttggca gaagtatatc tcagcgatac aagaaatgcc ttttacattg
    14161 gacgtggtgc tgactacaac gtttctcttg aagctgcttt gaaattgaaa gaaatcagtt
    14221 atatccaggc agaaggtttt gccgctggcg aattgaaaca tggaacgatt gctttgatcg
    14281 aagaaggaac accagtgatc ggaatcatca gtgaagaagt gactggtgcg catactcgag
    14341 gaaacctgaa agaagtcgaa agccgtggcg ctaaaacact agttattgtc tcagagggat
    14401 tagaaaagga aagtgaccag ctggtgctac caactgtcca cccttatcta actactctaa
    14461 ccacagtcgt accgactcaa ttacttgcat attacgcaac gctattacgg ggattcgacg
    14521 ttgacaaacc acgtaattta gcgaaatcag tgacagtaga ataataaaag aacaaggacc
    14581 agtgtcagac atctgacact ggtccttgtt cttttatttt ttatgaaaat atctcttaat
    14641 ccagtccgat ttcttctttg actactgccg cgattttatc gacatagtaa ttaactttct
    14701 catcagtagg cgcttctgcc atcactcgaa gtaatggttc tgtaccggaa ggacgaacta
    14761 ggattcttcc ttctgatccc atttcagctt ctgcctcttc aatcactttt ttgattgccg
    14821 gaacatccat cgcaccgttt ttatcactta cgcggatatt cactaatttt tgcggataga
    14881 ttgttacctc tgctgcaagc tctgataatt tcttacctgt ctgtttcatg atattcagta
    14941 actgaattcc tgaaagcatc ccatctccag ttgtgttaaa atctaagaaa atcatgtgcc
    15001 cagactgctc gccgccaaag ttataatcgt ttttgcgcat ttcttctaca acgtaacgat
    15061 caccaacttg tgtaacgaca tctttcaaac cgattccttc caccgctttg tggaatccta
    15121 agttactcat gacagtcgta acgatcgtat cttttttcaa tcgatttttt tctgccaagt
    15181 atttagcaca gataaacatg attttatcac cgtcgacaat gtttccaagt tcatccaccg
    15241 caataatacg atcgccatcg ccatcgaaag ctagtccagc atctgctcct ttttcaacga
    15301 ccatctcagc taaacgttct ggatgggtag agcctacgcc gtcattgata tttaacccgt
    15361 ctggatttgt gcccattgta taaaaatctg tttcaagatc ggcaaacaaa cgattaacac
    15421 ttgttgaagt tgctccgttt gctgcatcga cacaaatggt caaaccggaa aggtcaccgt
    15481 tgatcgtttg tacaaggaat tgagaatatt tcaataagcc ttctggaaat tcttctacag
    15541 ttcccaatcc ttcagctgat ggacgaggca attcatccac ttcagcatct aataatgctt
    15601 caatttctag ctcttggtca tctactagtt taaaaccatc tgcaccgaaa aatttgattc
    15661 cattatcttg tgctggatta tgagaagcag aaatcatgac ccctgcgctt gctttttgca
    15721 aacgtgtcag ataagcaaca cctggtgtag agatcacacc tagctggaac acttcgatac
    15781 ccaccgataa aagtcctgaa atcagcgctt cttctaacat ttgacctgag attcttgtat
    15841 cccgccctac taacacgcgt gggcgttttc cttcttcttc atgctggctt aggacataac
    15901 ctccgcaccg tcccagctta aatgcaagtt ctggcgtaag ttccttgttg gcttcaccgc
    15961 gtacgccgtc tgttccaaaa tatttaccca tagttgtaaa attactcctt taacttaatt
    16021 ttctgaggaa tttgttctgc tcatttgtgt tgattcgctt gtttctgatg tttcttctgt
    16081 acttgtggtc gagctgcttg tcccagattc cgaactggaa gaaatggttg ttgtcgatgg
    16141 ttccgttcct gaatcagcag atgaaatact tctactcgta cttgtatttt cttgtgtccg
    16201 actgctcgtc gaagcagagc tactgcttga cgtttgcaac acgggtgtaa tctccacact
    16261 aactgattcg ggcttgatac ttaacccctc ttctactggt atttcaattt tacgagttgt
    16321 tcgatgattg atacctgaga cattgatcgg tacagcaata ctgtcaccga tcgatgcaag
    16381 caaatcgcta gagccgctta cttctgcttc cagttttgaa aaactgaaat catagctttc
    16441 taccccggca gctggtgttc cttgctgtat cccataaagt cgtaccgact tgcttggctt
    16501 gatcgaatca gctctcactg ttacttgcac tggatcagag acgatactta atacttcccc
    16561 tgctgcattc agcgcctgta ttgggacctc actattaatc ccatcatctg tgaccatcga
    16621 agcgtccaca tcagctacta ctcgtttgat ttctgccaat gttttgtcac ctgtagtgat
    16681 agagactttt ttaggactaa cggaagtatc atcaagttca tatccagatg gtgtgaacgt
    16741 tgaaggaatc aacgtttcca catcgaaatc ttttattacc tttttctcaa ccgttactgt
    16801 aatagtcgat ggttcgatct ttgctgtgac agcactgctc aaattttgaa tttttagcgg
    16861 aacttcatgt gttccttcgc ccagcttagt gatatctgct gtgacgcgga acatccgtgt
    16921 atcttcgttt gcctctgcat tcaactgaac tcggtttgca ctggaaagtt tgacagtcac
    16981 tgcattttca tatccatgga tatagtagcg ctttgaatca taagaaatct tgatcggcac
    17041 ccccgtaact gtttgattat acgcttcatt tccagaaagc gtatttggaa cggactggcc
    17101 atttgcattg aagtacagcg caatactgaa cagcaatgca ataaaactgt aaagaatgct
    17161 tgtccgtttc tcttttgaga tcatttgcga tcccctccct tagtcatacc gtctaggaaa
    17221 gattgaatca cgtttttctt gttttttgtt ttctcttcag caattagttc tctacgcaag
    17281 attgccaaat attcttgctg ggataagcca gccatcagtt cattatctaa cgtgatgctg
    17341 acgtccccgg tttcttcaga gacaacgatg gtaatcgcgt cactgacttc gcttataccg
    17401 acagctgccc ggtgtcgagt cccgaattct ttagggatca acatactttc tgatagagga
    17461 agataggcgc ttgctactgc gatctttcct tcttttacaa taactgcacc gtcatgcagc
    17521 ggtgtattag gaataaagat attgatcagc agctccccag tgatatctgc atctaaagca
    17581 atccctgtct caatatactc atctaatcca gtatgacgtt cgatagtgat caacgcaccg
    17641 atttttcgct tggacatata ttggatggct ttgtcaaaag acaagatcat cttctcatct
    17701 tcctgctgtt cgctttttga ttgtttgaaa agcgaactgc gtccaagatg ttccagtcct
    17761 ctccggattt ccggctgaaa aatcacgata gcagctatca ccccgtacgt aatgacttgg
    17821 ttcatcagcc aagacaaggt gtgcagacca atgatttccg ctacgattcg gataacaata
    17881 aaaatagcta cacctttcag aagctgaatc gcttttgtgc ctcgcagcag ttggatcagt
    17941 ttgtatacaa gataccagac aaccagaata tcaagtatgt tcaccaaaaa attcagagag
    18001 aacaagtcac cggaaaacat ttgctgccaa tagtccaaat taaaaagttc cccgatttga
    18061 aatgacataa aaagcctctc ttctcttttg agatttcctc tctagtatat catatgtttt
    18121 aattttagag gctgatatgc tcgtattttt cttgagaaaa acgcataatt ttcaagaaaa
    18181 ttgcaagtat gcgttttatt tttctctgca ataaatattt ttaaacttca gagaattatt
    18241 cattatattt tttctgttat ttttcctttt tttcttttgt agcttgttcc atttttttac
    18301 gtcgttctgc tctggcagct cttctagcag ctcttctttc ttctgcttca aggtccgtcg
    18361 ttttttcttt tctagtctta acgggtacat cgatagattc ctctggttca agtcgtttct
    18421 tgattttttc taattctccc gtcagtgcag catagttgta aaaacgtgtt ttagcgtcgt
    18481 ggacagtctt ttcaaataaa gcttttgcaa gatttggatt tgcgttatct agcgaggaaa
    18541 agcgaacttg tttcctcatg aaatcaggca tagcagagaa atcaggtttt ttgtagtcca
    18601 aagtcattgg gttcttgttt ttttcgacca ttgtagggtt atatcgatat aatgaccaat
    18661 aaccagaaga aacagcttct tttgcttctt taagcgattc tttcattccg ccaagcaagc
    18721 catgtgtgat acaaggcgta taggcaataa tcagagaagg accaggatac ttttctgctt
    18781 cttcaaatgc cttgatcgtt tgcatttgat tggcacctga agcaatttgc gccacataaa
    18841 catttccata tgtcatcgcc atcattccta gatctttctt tgaagcatac tttccactag
    18901 ccgaaaattt agcgattgcc gaagctggtg ttgcttttga ggtttgaccg ccagtattgg
    18961 aatagacttc gttatccaaa acaaggatat tcacgtctgc tccgctggca agtacatgat
    19021 cgatcccgcc aaaaccaatg tcgtaagccc atccgtctcc accaatcatc cattggctgt
    19081 tttttacgaa tagatccttt ctcttgtata tatcctccag ttcagggaaa attgatcgtt
    19141 cgctttcgat cgcagcttgc agttttgctg ctctttgctg cgttccttca ctctcaaaca
    19201 tgtgtactaa ccagtcttcc atcaattctc gtagttcttc tgatactttt ggcaatactt
    19261 ctttgattga acttgccaaa gcctcgcgac gagtctgtgc cgctagaagc ataccataac
    19321 caaattcagc attatcttcc aataaagaat ttgaccatgc aggcccttga ccacattcat
    19381 tggtcgtata aggagaagca ggagaagcac cgccccaaat agaagaacag cctgtcgcat
    19441 tcgcgatcat cattcgatca ccgaacattt gtgtcaataa ttttacataa ggtgtttcgc
    19501 cacagcctga gcaagcgccc gagaattcaa gaagcggttg attgaattgt gtagacaaga
    19561 cagtattctt tcctttgact ggatttgctt tttgtcttaa tgtcattgca aaagcccaat
    19621 tcacagcttg ttctttctgt tcttcataag gcttcatcac taaagctttt tcttttgcgg
    19681 gacaagcctc tacacacaga ccacaacctg tacagtcttc cactgaaact tggatacggt
    19741 attttacgcc gtctgcgcct cgcatttctc tcacgataaa tccttctggc gcttcttcca
    19801 tttcctcttc atctgctaaa aatggacgga tggcagcatg aggacaaaca aaggcacatt
    19861 cattacacat cgtacagcga tcactaatcc actcaggtac ttctaaagcg acgccccgct
    19921 tttccataga agccgttcct aaaggcattt cgccagttgt cattccattt gctacaagtg
    19981 taccaacaga gagttgattt ccttcctgtg cattcactgg ttcaagtatt tcttgtatat
    20041 aaccagtgtt ccttttattg attttttcag gcatctccaa cgttctccaa tggtctggga
    20101 tcgcgacttg atgaagcagt tcggtcgtcc gatcgattgc ttgtatgttt ttttctacga
    20161 tagccattga tttatgtcca tagcttttca gggcttcttc ttttagcagc ggaagcacct
    20221 catcaaaagg aatgatgtca gctaatttaa aaaaagccgt ttccattgcc gtattgatcc
    20281 ggcgtcccaa accgacttct tgggccaatc tcatggcatt gatcgtatag aatctaatgt
    20341 tattttcagc caagtaacgt tttaatttta agggcagatg cgtttctaat tgttcgtcgc
    20401 tccaaagagt attcaataaa aaaatgccac ctggcttcaa tcctttgacc aagtcatagc
    20461 tatgcaggta agctggtgtg tgacaggcaa caaggtccgc atgctcgaca agatacgctg
    20521 aacgtatcgg cgtatctcca aaacgaagat gagagacagt cagaccgcca gattttttcg
    20581 aatcataata aaaatagcct tgcgcatatt tatcggtgtg atctccaata atcttgatag
    20641 ctgatttatt tgctccaact gttccgtcag atccaaatcc ccaaaacttg gcctggaatg
    20701 tttgcggttc tgtcaaatcc agactttcct ttggttccaa agattgataa gtcacatcat
    20761 ccacgatccc tatggtaaac cgttttttgc gtatgcttgg gtcttttttc aattcatcaa
    20821 aaactgctga gatttggtcg ggagtcacat ctttagaccc caatccgtag cgaccaccaa
    20881 taacagccgg gcgaagttca gaatcatata acgcactttg aacgtctaat aagagaggtt
    20941 ctccacctgc ccctggttct ttacttcgat caagaacagc aattgttttt actgtatgag
    21001 gcaacttttg cacgaatgtt tccactggaa aaggacgata aagatggata ttcaaaaaac
    21061 ctgttttccg tccgtttgca ttcaagtaat caatcgtttg ttcaatcgtc tgtgcaactg
    21121 atcccataga aacaatgact tcttccgcat cgtctgctcc gtaataagtc accaagtcat
    21181 aagaagttcc tcgtaatcgg ttgatctctt tcatatattt ttgaacgatt tcaggtaatt
    21241 tatcataata ggggttgatc gtttcccgtt gttgaaaatg gatgtctgga ttctggttcg
    21301 taccactaac agtcggatga ttgggattca tccctcgttt acgaaatgaa gctaatttct
    21361 cctgattgat cagaggagct aattcttcat attccaaaac ttcgattttt tgaatctcgt
    21421 gactcgtacg gaatccatca aagaaattca aaaaagggac ttctccttca atagaagcaa
    21481 gatgggcaac aggtgctaaa tccatcactt cttgtacact gctttcagct aacatagcaa
    21541 atcctgtttg tcttgcagcc atcacgtctc cgtgatctcc gaaaatattc aacgcatttg
    21601 tcgtcactgc ccgactagca acgtggaaaa cacttggcag tagttcccca gcaatcttgt
    21661 acatattcgg tatcatcaaa agcagccctt gtgaagcggt atatgtagtt gtcagtgcac
    21721 cagttttcaa tgatccatgg accacaccag ctgctcctgc ttcggattgc atttctacta
    21781 cttttactgg ttgaccgaat acgttttctt ttcctgctgc tgaccattga tctactaatt
    21841 cagccatggt cgaactgggt gttattggat atattgcagc aatctcggta aatgcataag
    21901 aaatatatgc ggctgccgta ttgccatcca ttgttttcat ttttcgcatg attcatcatc
    21961 cttgttcata ttctctctta ttatctgttc cattataacg taaaacatgc catttttttc
    22021 ttttatttat aaaaatattt tacatgtatc tatttgaatt ggtcatctaa tttttagaca
    22081 aacagtctaa atcgttgcct ttttttgtca gacatgtata aactataagc gaaagaggtg
    22141 ttttaacatg gatatatcag tgattgatgc aacgaaaaca aatacacaaa aaggaatcct
    22201 ttatggttca tcgaatgctc ctaaaaaaat ggtcgaattc atcaacttag cttgtccgta
    22261 ttgccgacaa tggtttgaag aatcttatga gctattagaa gaagcagtcc aaagcgggca
    22321 attgcagcga gtgatcaaat tgttcgataa agaaaaagaa agcttacagc gagggaacgt
    22381 gatgcaccgg tatctcacaa tcagcgatgg ccaaaaagcg atcaaagaga tcaaacaaat
    22441 attcgatact caagacgagt ggaaacatct ttctctccaa gaagtagcgg attttgcagt
    22501 agataaacta aagctgacag aacaaaaaga cgaacagctg agccaagctg tcatcaacga
    22561 agctgagcaa gcacatattc gatttgttcc aactgtcatc ttgggtaaag aaatctttga
    22621 tgaatctatt tcaatagagg aattaaaaga attgatccaa acaaaataaa aaaataagga
    22681 gcgtgggaca aaagtaaaga acacttttgt ctcacgctct aaacacgtat aaacggcgga
    22741 agcagaagca actccttctt atttcttgga gttaaacgct tctgtcccag cctccttatt
    22801 ccgaataaat ggtgtccaaa agcttgcatc tgccagatta tctcatttct tatgacaagc
    22861 taagcaaatt atctccacca aataatttat tcatattttt gacttgcgca tactaactaa
    22921 ttcttaaatt ttcatacaaa atttctcaat ttcccttaac gagtaatatt tatgtacggt
    22981 gattctcact agtatcccaa tcacttttta agttatcccg atttcaattc tacttcaaaa
    23041 atagttgctt ttatgtatgc tgttagttga ttagatggtc atttgttgag ctaggtcgtt
    23101 tattttaaac aagttgtttc tttagcttat acctatctcg tctaccaaac gcaagatggc
    23161 aaacacaaat gcatacgcat ctgtatttgc cactttgatt cgtataaagc taacaactat
    23221 taactcctct cagctaatag ttgctctctt ttttattcag attcattttt gctgatcaca
    23281 gcctagtatt tcaatggttt tgaacactaa accaaattgt tccattacaa cattcactgg
    23341 ctgatgaaat ggattagtag atttcgatta ttcaaagcga ttttctattg gtggatcgtg
    23401 ttcccaacta ataagacgga ttccaaacct atcgtacaga tcttctaaac cacttataaa
    23461 ttttgcacct atttcaggtg ttttattgcg tggatattct attgttacgc cattaaatgg
    23521 ataccaatta ggattaggct ctcttttttt ctgtacatct cccatagaat agttagcact
    23581 ttctgggaca taatatattc ttcgaccatc ctctaattca tctaataaga tatttcgtga
    23641 tcgtctatca cttgctaaat gtagacgcca cgtgcgatac catgccatgc tacctgcatc
    23701 tgcagaaaat ccatcaaata atgcaacaat tgtatcttct atcacaatcc tttccccatc
    23761 tacttcatat tctaatctaa aatcaaactc ccctcgggta atttctggtc ttggtggctt
    23821 aggacccaat attattctac ttaaaaccgc attcgttccc agatgaaaca cagctataac
    23881 aacaataatg attaatattt tgtttctatt tgataaattt ctcattattc gcacatcacc
    23941 tctacattct cacatattag ttcgtaatac tcctttaata tcaaaatatc tactggtcgt
    24001 aataatattc gctatgaatt cctactactc gtataagaaa ggccaaatca tcagctttta
    24061 cttgctgatg atttggcttg tttctattta tcgtaattgt tttctaatca ccaaaattgt
    24121 taacaatcgg ttcagatatt tcccagttga ttaaggtaat tccgtattgt gtcaaaactt
    24181 cttgcgcttc tccgaaatta agtacagttc ttatgtatcc actttctcct aatataaagt
    24241 ctacctctct tttaaaacct tcctcgagat ttctaagggc tatatgtggt tttaatgaca
    24301 ttgattgtac attttccccc atataatatt gcgcacttcc tcgactaaaa tagattccta
    24361 gatcgtcccc aaaaggtaga tcttctctac cactagctaa tctacttctc catcttcgtt
    24421 ctcttttcat agaacctgcg ctgaactcac acccgttaaa ctcagctatg acagtatctt
    24481 caattatgtt aatctgtcca tctagttcgt attcaatcct aaatggaaat tctccatagg
    24541 tgatttccgg ttcgggtatt ttaggtgaga ttaatgcaaa gaaaggaaat attaagcttg
    24601 gcaacatcaa aaaaactcct cctatgatca caattcccga tgtaatccaa aatataattt
    24661 gtacaattct cattgatcca ttgtattcca ttttattccc cctattacta ttcatatccg
    24721 cctatctata tcgatcttcc aatgcagaga taattgcttc cataccttga ttttttactg
    24781 agttacctat gcgagatatc attctccact aaggtgcatc atattgtcta tctaacatga
    24841 ttaattctcc aataggtgta tactcctcca aaaacaacat ttaatgcatc tccctcgaca
    24901 acataagcta ccatgtttgc tgtatatgtg tttgagtcta attcataagc agataaattt
    24961 actgtcgctg gattaaatat aatcgcattc gtatttatag ctactgaatt tgctgcttca
    25021 gcaccacctt ttgaatgtcc gatcattgtg atttcaatag taggatttac gtggacagcc
    25081 ctcaccgttt accctgacca ttatagaaat cgcttacttt tttgaactac gcaacaaagg
    25141 atacaatgct cctaacttca ctgtctccat ttttgtccac taaggaatat aaaaaatagg
    25201 cattaattca tttaattcgg tcacaacacg ttacctttta aatcattata ctgtatctca
    25261 tacattatta tcaataaact attataaaaa tgcaaaaaat gctttatcaa tctattttgt
    25321 aattggtcat aatatcattg aaatcatacc ctgtatcgat ttttcctatg aatctcgtct
    25381 atttgtgatt gacgaaaaag aggcaagaca gacactggaa tggtaatttt acccactatt
    25441 ttgctccaac tatttgtaat cgtacccact tttgaaatac cgagatgatc attttaattg
    25501 atatgctgat tcccaagcaa aaccccaaga aattaaatgc tctatcgcat ctagcttctt
    25561 gggttttgct tatataaatt ccaacattta aattttttat gctgttcggt ctcagaaatg
    25621 ctgtttttgc ttatatcaca atatcaagat gtcaaattat tcgtgttgcc gatgactttg
    25681 attaattttt ttcaaattgt ttattaagac taatgctgtc tttttgcaga taccaatgtt
    25741 cgaacttttt aacattatca tatgatcctg caagttttaa cttcttacca ttagatgtta
    25801 tcaaagttat aattaacctt gcctttttcc cattaggatc ttttatttta tgaattttct
    25861 gcaggtaatc tctcctaatg gcagctattt ttacctcatc agtgctgata aaccattgat
    25921 catccacata catactgcct gttatgttgt gtttttccat ttcttccgag aacgaaaagt
    25981 ttaaggctcg ttcttacttc caaagttgaa tgagtccatg gattggactt agtgtgagaa
    26041 ttgagaacaa caataagaat ccatatcctc ccgcaaacat ccctaaggct atactgtctc
    26101 tccatccata agtaggatta gaagagaaaa gagtcaccgt ttgtaataat atgaaaagga
    26161 ataatccagc acaaacacca aagataacaa ctccaaacat tgaattttta tacaatgacc
    26221 atttatttac aggcttttct ttcattttac ttccccctac tgttctgttc acacggaatg
    26281 aattcaaagt tcataagccc tcctctttga acatatgaat acgacttgct aactatactt
    26341 taaaaaagct tgttttaagc ggttataggc gagttgtaat ctctattcat ctcattagca
    26401 aataacttgc cgaaaagtct actgaaatta aagcagatag tggaagaatt atttagccag
    26461 gatagacact atttgtgaaa cacatgtctt tacttttgaa attctatgat gaaaagcctt
    26521 ggaatacagt agctcttaat tggtaatact ttttccatta taaataacct cttttcattt
    26581 tatatttatc ccctctaaaa ataatatcta atctaatatt ttaaacttat atttccctcg
    26641 ctgtgctaat agagcattac taaataaata gaaactagaa aaaagcatta cgcctgccag
    26701 aataaaccaa ttatttgtat
//
LOCUS       DAKBVG010000011        24613 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000011 DAKBVG010000000
VERSION     DAKBVG010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 24613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.011"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(338..2230)
                     /locus_tag="OUD09_000394"
     CDS             complement(338..2230)
                     /locus_tag="OUD09_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT9691700.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2227..3954)
                     /locus_tag="OUD09_000395"
     CDS             complement(2227..3954)
                     /locus_tag="OUD09_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT9691701.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3970..4620)
                     /locus_tag="OUD09_000396"
     CDS             complement(3970..4620)
                     /locus_tag="OUD09_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HCT9691702.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4824..5120
                     /locus_tag="OUD09_000397"
     CDS             4824..5120
                     /locus_tag="OUD09_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCT9691703.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5312..5932
                     /locus_tag="OUD09_000398"
     CDS             5312..5932
                     /locus_tag="OUD09_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCT9691704.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(6024..7190)
                     /locus_tag="OUD09_000399"
     CDS             complement(6024..7190)
                     /locus_tag="OUD09_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCT9691705.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7202..7555)
                     /locus_tag="OUD09_000400"
     CDS             complement(7202..7555)
                     /locus_tag="OUD09_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCT9691706.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7683..8705
                     /locus_tag="OUD09_000401"
     CDS             7683..8705
                     /locus_tag="OUD09_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HCT9691707.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            8724..8885
                     /locus_tag="OUD09_000402"
     CDS             8724..8885
                     /locus_tag="OUD09_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9691708.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(9144..9578)
                     /locus_tag="OUD09_000403"
     CDS             complement(9144..9578)
                     /locus_tag="OUD09_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9691709.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9688..11547)
                     /locus_tag="OUD09_000404"
     CDS             complement(9688..11547)
                     /locus_tag="OUD09_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HCT9691710.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(11665..12824)
                     /locus_tag="OUD09_000405"
                     /pseudo
     CDS             complement(11665..12824)
                     /locus_tag="OUD09_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
     gene            complement(13131..14555)
                     /gene="araA"
                     /locus_tag="OUD09_000406"
     CDS             complement(13131..14555)
                     /gene="araA"
                     /locus_tag="OUD09_000406"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HCT9691711.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14571..15269)
                     /locus_tag="OUD09_000407"
     CDS             complement(14571..15269)
                     /locus_tag="OUD09_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCT9691712.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15226..16887)
                     /locus_tag="OUD09_000408"
     CDS             complement(15226..16887)
                     /locus_tag="OUD09_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HCT9691713.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            17105..18505
                     /locus_tag="OUD09_000409"
     CDS             17105..18505
                     /locus_tag="OUD09_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HCT9691714.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18757..19836)
                     /locus_tag="OUD09_000410"
     CDS             complement(18757..19836)
                     /locus_tag="OUD09_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCT9691715.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19922..20881)
                     /locus_tag="OUD09_000411"
     CDS             complement(19922..20881)
                     /locus_tag="OUD09_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HCT9691716.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20893..22404)
                     /locus_tag="OUD09_000412"
     CDS             complement(20893..22404)
                     /locus_tag="OUD09_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HCT9691717.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22426..22989)
                     /locus_tag="OUD09_000413"
     CDS             complement(22426..22989)
                     /locus_tag="OUD09_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HCT9691718.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(22995..23858)
                     /locus_tag="OUD09_000414"
     CDS             complement(22995..23858)
                     /locus_tag="OUD09_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCT9691719.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23872..>24613)
                     /locus_tag="OUD09_000415"
     CDS             complement(23872..>24613)
                     /locus_tag="OUD09_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCT9691720.1"
                     /translation="NFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAV
                     ALSKNTRFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFR
                     DPTLAMPTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQ
                     IVPSLGFCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASA
                     LSSVLFIIVATIAISTNVYMSRKEKQMK"
BASE COUNT         7179 a         5165 c         4346 g         7923 t
ORIGIN      
        1 aaactggctt ttgaacaccg tttaccaaga ggttgtgagc ctgccgttta actccgagtc
       61 acaaggagca atttcaaaaa acagcttctc aggtttgttg actattgtga cttgtgaccg
      121 aggagttggc aggagaacac cgtttaagga agaggttgta aaatcagcgg tttgacctga
      181 gtattagaga tacaaaacgg aaaaatagct tctcatattt tgccgttttt tgagctaata
      241 ccgaaggaca gctgattgaa caccgtttat tcggaaaaag ggactgtgac acgacttttg
      301 tcacagcccc taaaaaaaat aataaatttg tactattcta tgaagcttct tcttcagaaa
      361 actgactgtt ataaagatct gcatagaatc cgtctttttc catcaatgta tcgtggtttc
      421 ctgtttctac gatcgaacca tgattcatca caataatgtt gtctgcatcg cgtattgtag
      481 acaagcggtg agcaacaacg aaacttgtcc ggttttccaa taggcggttc atcgcttttt
      541 gaatcagtat ttctgttcgt gtatctacac tagatgttgc ttcatcaagg atcaggacat
      601 ctggatttgc taagaatgcc cgcgcaattg tgattagctg acgctgccct tgcgaaatat
      661 tgcttgcatc ctcgtttaat acagtgttat atccttctgg caatttacga acaaaatcgt
      721 ctacgtgagc agctttagca gcctcaatga tttgttcgtc tgttgcatcg tcgttcccat
      781 atttgatatt gtcatagata cttcctgtaa acagccaagt atcttgcagt accattgaaa
      841 actgtgcgcg caattcatca cgtgaaagat cacgggtatc tacaccatcg taacgaatac
      901 tacctccaga aacatcgtag aaccgttcaa gaaggttgat cagtgtcgtc ttccctgcac
      961 cagtcggacc tacaatagcg accatctcac caggtttgac atccaagtta aaatcagtca
     1021 ttagcaagtt atctggtgtg tagccgaatt gaacattttc aaaacgaact ttgtatggtg
     1081 tatctttttc ttctggaagg ccagattttt cattggacat ctcttcttca tccaacactt
     1141 caaatacccg ttcggctgaa gcaactgttg attggatagt gttcatcaaa cttgcaagtt
     1201 gagtgattgg ttgagagaac tgtgttgtat attgcaagaa cgcttgaaca tcccctaatg
     1261 tcatgttccc atttgcaact tttactccgc ctagtacagc gacgaacaca tagcctagat
     1321 ttttaacgaa attcatcaac ggcatgatga ttgccgaaat aaattgagct ttccaaccag
     1381 cggcatacag attctgattt tctttttcaa atacttcctg gtctttttca gtatgattga
     1441 atgttttaac aatggtatga ccaccatatg tttcttctac ttggttattc aataaaccta
     1501 aacttttttg ctgatctgcg aaaaacactt gtgatttcgg cgctacgatc atcacgatga
     1561 tcaagcttaa agggatcatc aataacgcga ctaacgtcaa ttgccagctg attgaaagca
     1621 tcatcacgat gaccccaatc aatgtgactg cactagttac aaattgagtc aaattttgct
     1681 gcagcgtgct tgcgatgtta tccatatcat tgattgctcg agacatgata tccccatttg
     1741 tatgtgtatc ataataggaa atcggtacac gattcatttt ggcttccaaa tcatgacgta
     1801 attcataaac tgttcgttgg gaaacacgtg tcataataaa ttgctgtaaa aagttgaata
     1861 cagcagaaat caaatacatc acgattactg tcgcaatgat ctgcgcgatt ttatcaaagt
     1921 ctatcggaaa ttttgttact tgttggcctt gttgcatctg ttgagcgcca gccattacac
     1981 ctttgaatat ttctgtggtt gctttcccca agacctttgg tgtttggatt tggaagatca
     2041 cggctgctat tgctagtaca aatacagcga tgatcgcaac catacgttta gacatatagc
     2101 caaacaatcg gcgtaccgtt ccccagaaat ttttaggctt ttcacctttt acaccgagat
     2161 ttctgccagg tccaccatgt cctggaccgc ctgctggtcc atgagaggga cgtgtgtttg
     2221 ttttactcat gctaagtcct cctctcttag ttgtgaatga acgatttctt gataagtttc
     2281 gtttgttgct aatagttctt catgtgttcc tttgccaacc agttttcctt catctagcac
     2341 aaggatcata tctgcatcca ttactgtact tactcgttga gcaactaaaa cggtgatact
     2401 ttctttcata ttcgttttca atgcttggcg aagattggca tccgttttga aatccaacgc
     2461 tgagaaggaa tcatcaaaca catagatatc tgctgttttg actaatgccc gtgcaattgc
     2521 cagacgctgt ctttgtccac cagagaagtt tcctcctcct tgttccacat ggctatctaa
     2581 gccatcttcc atctctgaaa caaaatcttt agcttgtgcg atttctaatg ctttccaaat
     2641 catttcatct gttgcatccg gcttaccata ctgcatattg tctcggatcg ttccagagaa
     2701 cagaactgct ttttgaggaa caaagcccat taattctcgc aaattatatt gagtcatgtc
     2761 actaatgtcc gttccattta tttggatcga accagattcg atgtcatata atcgagggat
     2821 caaattgact aaggtcgttt tacctgaacc agtcccccct atgattgcga cgatctcgcc
     2881 cgacttggct tggaaatcaa tatcttccaa tgcaagtttt tctgctccat gatatcgata
     2941 attgacatga ttgaaggcca atgttgcgtt ttttccagca aaagaaactg ttttaggatt
     3001 ttcagggtca tggataccga ttttttcatc caacacttca ttaatacggt cagctgaagc
     3061 ttgtgcacga ggcaccatga taaaaatagc ggaaagcatc ataaagctga tcaagatctg
     3121 catcgcataa gtcataaatg caatcagatt accgacttcc aaagtcatat ctgctatgta
     3181 gtgtccacca aaccaagtga ttgctacgtt tgtaccgctc atgatcaatg tcatcattgg
     3241 catcatcaat gcgacgatcg tattaacttt gattgccgta tttgtataat ctttattcgc
     3301 taaatcaaaa cgattttctt caaagcgtgt cttatcgaat gctcgaatca ctctgacccc
     3361 tgtcaatcct tctcgaaata ccaggtttaa acggtcagtt ttcttttgca tacttttgaa
     3421 taatggaaca gcaaagtaca tgattccgcc gatcaaaata atcattactg ggatgacaac
     3481 taagaagatt tttgtcaact gatgatcttt ttgataagca agtatgctcg cgccaatcaa
     3541 cgtgattggt gcattgatca tcatacgtaa aaacatttgt gtaaccattt ggatttgagt
     3601 cacatcattg gtcgttcttg tgatcaaaga ggccgttcca tatttatcaa acgcatcttt
     3661 tgtcagactc tctgattttt tatagacatc cgtacgcaat tgcttaccca gtttctgtga
     3721 ttcacgagta gcaaaaaatg tatttgcgat agcagctaag atactgatca aagaaaagcc
     3781 gatcatcaca aaacctgtat gccaaatata atcgacatct ccttgcgcta cccctttatc
     3841 aataatattg gaggtaagtg tcggcaaata taaatctgcc agtacttgga tcaccatgaa
     3901 aactgcagct gcaattgctg aagtcaagga gatccgtttt acgattttta gcatctagtt
     3961 cccttctttc tatttatata ctccgttttt cagccaagaa agtttcaaat tgaattcact
     4021 gattgcttgc tctactgagt aatcttcaga tgttgaaaat aagcgatacg aatgcgcaat
     4081 tgttgtaaat acagtattca tcactaattg gatcagtaat tccaattcat gatcattttt
     4141 tactttcagc ttcgtccgat cgatcaattc aagcaactct tttgcatctt gtttgtgttc
     4201 tttatgtgga tcgtgaggcg ttttttggcg actcgtatga ggagcgactt ttcttgaggc
     4261 acggtaatcc atgttcataa acaagttacg ataaaaagaa gcattctcgc cttccaaaat
     4321 ctcataaatc attttagaaa aataggattc gaatcctttg aatagatcac catcggattc
     4381 tttcattagt tgtaacatat ctttagagct gtcacgtctc attgtctcaa agtaataaaa
     4441 ataaagatcc tctttatctt caaaatactg atagaaactg cctctaggga tatccgctaa
     4501 tttgacgata ttagcaatcg aagcttcttt taagggtact ctagaaaact cgattctcgc
     4561 agcctcgatt agtctttttt gtttttcttt aggcaaatgg aaaaatgtct gtgtcggcat
     4621 atggattctc ctttctttca agatgacaca ttgtcatatg acgctatgtc attataacac
     4681 ctcctacctg ttttgcaaga catttgcata gtttttttca ttatcatccg atacattaaa
     4741 aaagtgataa gaggaatcct tccatttgaa ttaatgagag ttgcccatta aaatagccac
     4801 atactaaaca aaggatgaga taaatggccc aatcaattag tgtccaagac ttcaataaat
     4861 tgcctctcga tgctgacagc atcgttttag atacaagaaa taccttagat tacacaaaaa
     4921 accacttaag ccactcgctt tctatccccg ctgctatctt gccaagacaa ttaaaaaatc
     4981 tagatccaaa gaaactttat tatattctaa gctatacagg caggcgatca gaagcattag
     5041 ccgatcaact tactgctaat ggttttcgtg ctgtttatat cattggtggg atggaagctc
     5101 ttcagtcgag tgttgcttga atattgcttg ttagattcaa aagaggctgt gacaaaaaat
     5161 cgtgtcacag cctcttttat accctgtctt tttttagtta tttattcttc aaccttatca
     5221 atatgacttg atagcctttt ctttttcgag taaactgatt ttgttagtca tagttataac
     5281 aatcatagca ataaaaggag aaggtaagca tatggcatct gttttagtag tcaaaggtca
     5341 tcccctaact gcagaagaat cgcgcacagt taaggcattg acgtcttttt taacaagtta
     5401 taaagaaaat catccagatg atgaagtgac tgttttggaa ctttatcgcg atgatattcc
     5461 ggaaatcgat gaagaattat tatcaggttg ggaagcatta cgtgctggtg cagaattcac
     5521 ttccttatca gaaagtcaac aacaaaaaat tgctcgtttc aatgaattga ctgatcagtt
     5581 ccttgctgct gataaaatcg ttatcgcaaa cgccttatgg aatctaaata tcccaacaaa
     5641 attaaaagct tggttcgata ccgtcaatgt agctggaaaa acgttccgct atacagaaaa
     5701 cgggccagaa ggattagtga caggtaaaaa agcactgcac atccaatcta atggcggtgt
     5761 atacaacgga caagactttg cctctcaata tgtaaaaggt attttaaact ttgtaggcat
     5821 cgatcaagta gatcagctat tcatcgaagg aatcgattat gaccctgacc gtgcagatga
     5881 gttgatgcaa aatgcattag ataaagctgc tgctttaggt aaatcatttt aaaaattgag
     5941 ataaatgcat acaaaagaga cagaggaaaa aatcttcaaa gcagcatttg tgccttggat
     6001 attttcccat ctgtctcttt ttgttattct ttctgctttt tcaacggatt ttcttcataa
     6061 agagaagaga ttgttgtgta ccattcaaaa acatgagtaa tcactacttt agctgctgca
     6121 taaacaggaa tccccaagat cacaccgatg attccgaaca atttcccaga agtcagcaga
     6181 actaataaaa tcgttactgg atggattgct aactgattcc ctagtactaa tggagagatc
     6241 aagcgtcctt ctattgtctg ctctactgca aaaacgatca gtactttgat gatcatgaat
     6301 ggacctgcta caatggctaa aaagatagcc ggaatcatcg ctaaaaatga tcctaagtaa
     6361 gggatcaggt ttaaaaaacc cgcaataatc cccaacgtga cagcatactc taaaccaatg
     6421 accgcaaaac caatcataaa catgattgcc acagcaaaag ccacagttag ttgtcctctt
     6481 atataagaag aaacttgact attcatttca ctcagcacat tcaacgtcgg tttttgcatg
     6541 cgtgtcggta aaaatttgac aaaataaggt gctaattgtt taccgtcttt caatagataa
     6601 aataagataa acggcatcgt cagcagagcc actaaaatag tcgctaccgc gccgacaaaa
     6661 cttccaagac tctgaactgt actcttagat atatcttgga tcatatcgcc tgccgaattc
     6721 atgaacttat cacctgctgt ttccaactgt tcgcgaaatt ggttgaacag cgggtcccgc
     6781 aaaatctcgg ttaatttatt atcaatcgta tctacatatt gagggaagtt actgatgaag
     6841 ctcactgttt gttcctgaat cttagggatg atcactacgc tcccccatgc gattagtgca
     6901 acaaccaaaa caaacagccc aatgatactg taaagccttg gtacttttcg cttttcaaag
     6961 taatcaacta ccggattcat caagtaatac aagattcctg ctaaaatgat aggcagacct
     7021 acgattgcta aaaattgcca gattggtgca aacaaatatg aaacttttgt aaataaaaag
     7081 acgatcagca atattaacaa tacaacaagt aaggctgtga ctacttgatt atttaaaaac
     7141 cagcgccaaa accacgatac cggtctgttc ttcttatttt ttccttccat aattcctcct
     7201 gttagttata aaaaatttgg cttcccaaac caatagcagg cagtctatgc ggatgaagat
     7261 agattccatt tgcaatttgt ccttcttctg gacaagcatc aaaaatcaat gatacgtgag
     7321 ctactgtctt taaattccca ttggccatcg gaccgacata atcgatcgta tatacctgat
     7381 catcaaaagc aattgttccg cctgctttca aagaaaaagg ttcgtctttt gatacttctt
     7441 gaatcaccga atattttcgt aaggcagctg ttgctgtttt atcgaacaaa atcagcatgg
     7501 gttcttcctt gtttattgct tctgtcccaa tttctgtaat ctttgcctga atcattgcac
     7561 tctctccttt aattgctttc aaaaacagta taacacattc tcattcttta gccgtgtgga
     7621 ataattatgt gatagaataa aaacgaaaac tatttcatta caattaggag gaatcagctc
     7681 atatgatcga aaagataatt ttaggaacct atacgcgtcg cgaaagtaaa ggaatctata
     7741 cgatcgacct tgatacagaa aaagaagaat tagccaacct ttcattagct gttgaagaaa
     7801 atagccctac ctatgtagca aaaagtaaag ctggcaatct ttacacagtt actagtgtgg
     7861 atggattagg tggtgccggt gcgtacgata aggattttca ttttttgaat gcggtaacag
     7921 agtctggcgc tcctttgtgt tatgtagcgg ttgatgaagc acgtcaatta ctatacgggg
     7981 cgaactatca taaaggtgaa gtaaatgtct accatatatt aggagatggc gaactgaaag
     8041 cagcagattc aatattccat caagaagcga caggtcctca taaaaaccaa gatcatgcgc
     8101 atgtccacta tacggatctg actcctgacc aacgattagt cgtatgtgat cttggtacag
     8161 atcgtgtgta tacttatgat gtgtccgaaa acggcaaatt gaatcttgta actaccttca
     8221 ctgcagagcc aggaacaggc cctcgccatc ttgtctttca tccaaatgga caattcgctt
     8281 atttatttgg tgaattagac agcacagtcc gcgtgctttc ttataataaa gaaacaggtg
     8341 cttttgaaga attacagaaa atatcgacct tgccagaaga attcgacggt gaaaatggag
     8401 gggcagctat ccgcatttca aatgatggca agttccttta tgcttctaac cgaggacata
     8461 actcgattgc tgtattcgca gtttttgaag atggttccca cattcaaaac aagcaaatca
     8521 tctcaacaga aggggatttt ccccgcgatt tcgcactaga tccaagcaat aattttgtcg
     8581 tgtgtgccaa tcagaacacc gataatctga cattatatcg ccgtcatcct gaaacaggat
     8641 tactggaatt actgaaaaaa gatgtctatg taccagaatg tgtctgtgtt tattttgatc
     8701 gttagtaaga aaaggagaat gaaatgaatc agcaagacgt attagacaga ctacgggaag
     8761 aactaaatat tcctttcttt gatgggaaaa tcgaagataa agagtatagc gaagaggaat
     8821 atcagaaact caaagccgat ttacagaact actttgaaca atatgtacag aatgtagaaa
     8881 actaatcagc tttccatact atctgaaaag aacatcagga caagactttt ccgtctatcc
     8941 tgatgttctt ttctacaaat aaaaaaccgc cgagatggtt agactcgacg gaaaaggagt
     9001 atttactcga ttttgagtaa gtacaaggtt gttgttggtt ataggtatgt cattatcata
     9061 aaggattttg atcactctat cataaaggta atattacatt tatattagat aactctagtt
     9121 tttattgtta tcaaagattt cttttatttt ttcaacaaca aattagtcat tttttggatg
     9181 gcagctgtca attggtctgt cgcttggtct tgttttgctt cagatgtatt gaatagatct
     9241 tcatctaccc ctgattcaag atcgatactt tggatcattt cttcacagcc tgctacatat
     9301 gaaagataag ctttttcaaa atttttatga atgcccattg cttttgctgg tggacgcaaa
     9361 gtactgactt tcataagcat tgcccgatat ttttttgttc cttcttcaaa taacaggatg
     9421 atctcttcaa gagtcgattt gtccagttca cttgtttttc cttcatcaat tgcgtttctg
     9481 atcacttcat atttcggatg cattccttct ccgattgctt ctgtttcctt gacgatttga
     9541 tcgatggact gcacataata gccaatattc ggtaacatgt tcgttttcct actttctcca
     9601 ttgttgttat tatttattct acttgttttt atcctcaaat gacaagtact tgaaaaaatt
     9661 taatggtttc tatacagctc gccattctta ttcgtgctca aagaaaccat ctgctaaact
     9721 atattgtttc cctgcgacaa tgtcatattg gatcaattga tattcgtcgt aaatctcttg
     9781 ttgcgagcga ttgaattgcg cttctttata ccactgtccg ttttgatagt acagttctcg
     9841 ttcaaacgcc ggaagttcct gttctaatga taaaagcagc tgataaaagc catttgttgt
     9901 ctgattcgtc tgttccatta gattcgctgc aaaataaaac ggactggtga ttgcatcgtg
     9961 agtcgtttgc ttttctaatt ctcctttact gtcgaacata agaaattgtg tttcatgcaa
    10021 tgtttgtttg tcattttttg cttggatcgt atctgaatag atccctggaa gatgatcccc
    10081 ccaaaaaaca actaaagtcc ttctagaaag attcttcaat tcttccgtga actgcttcag
    10141 tgccgtgctg ctataggaaa tatcttgcag ataattctct aagtcaagtg tcccacttcc
    10201 atctgaagtt tttgcaggat agtctagatt atcatacttt ccatcgtagg gcatatgtgt
    10261 ctgcatcgtg actaaatgaa tgaactgagg tgttttttct tccttgagaa gtgtcattac
    10321 ttctttgtaa gctgactcat cagaaatata tggattattc tctatcgtat ccgtataggt
    10381 catcgtacgc tcgctgatga actggtcaaa tcctaatgtc tgataaacat cctctctttt
    10441 atacatcgaa gtattgtatg gatggatcgc tgtcgtctgg tatgattgtg cgtttaacgc
    10501 tgaaacgata gaaggaagct gatccatttt tggtacaagc atcgtgtaag gggtggtgag
    10561 ttgcgcatta aataacgcca tagaaaaacc agtcaatgct tcaaattcga tatttgctgt
    10621 tccaccgcca tagttttgag aaagcatatt gccactatat gtttgatcag ctacttcata
    10681 ataatctgcc aatggctcac cagatacttc tacgccgttt aatcgactag gatctgagaa
    10741 actttcactc atcacgaaca cgatgttcgg cgactcttcc tctacagttt tatttttttc
    10801 atcggctagc ttctggtatt tttcagtgat ttctttgatc ttttctttcg aatatccttc
    10861 aggttcatcc attggttcta ccttcagatt gtacaaaaac ccaccgataa aaccagtgtt
    10921 atagtagttc attttctggc tgtaaggtat ccatagtgcc gttttattat atgcttttcg
    10981 caacaaatta tcgggattgt tgaaattact gatataccct agcagtccaa ttgccacaag
    11041 cagtgtcgtt aatcggatca attggaatcg ccttcctttt ttcaggcttc gaaacatgta
    11101 ccagcaaaac aatcctatca cggttcctgc ggcagctaag atcactgtaa acagcatagt
    11161 gccggtcata tctttcagca atccgatttc agtgatcatc ttcaaatcgt ctggataaat
    11221 cggttcttgc cgataactca ttttcatata atttgcaaaa cctaatacac cgatggctac
    11281 gacatacgta agggaaccta aagggacaga tcctgctagt gcaatcagaa aaataagaat
    11341 aatcagcaag acgagacagg ctaagaagaa tttttccgta tgccaagaaa aagcaaattt
    11401 caacgcaagg tctacggata gatcattttg acaccactgc aaaaataaat tactaaaaac
    11461 aacaagaaaa aagatactga tgattgctgc tgttcctttt aaaatctttt tctgtttggt
    11521 cgaaagtctg cttatatatc ttttcaaatg cttgctcctt catttctatc aataaaagaa
    11581 tgttgcttct cttattttag ttgtacgaaa ttcgaaagta aagaacggaa agaagaatta
    11641 atgaaatcca aatattctct ttctttatgg cctttctggt ttctttaata agcggacctt
    11701 tcgtactcca gaaatttttt ctagctgttg tttcacttga ttgagctgtt caaaatcagt
    11761 actgtctatg tcaatcaata tgtagacatg ctcattctta tggttacgcg atatatctgc
    11821 gatcgctagc tcgttctcat tcagcagaga aaatatctct gctaatatat tggttttctt
    11881 ttgataaaat atagtgaatc gaaacggcgc ttggaaaaac attcgtgcgg atggataatt
    11941 gaccgcttct ctaaccgtac cgaacaaaag ataatctttt aaggcttcaa ctgccaaacg
    12001 tccgctgtct gccaaagctt cctgagtcga tcccctagat gaggcaacat aagtatacgg
    12061 tcattttcta agaactcttc ttctggaaaa tcagtcaagt atttagccag atgttgtttt
    12121 tccaatgctt gcataacagc atatttatca acaatttcac ttcttcctac attgataagt
    12181 acagcatctt ttttcatttt ttctatattc gcctgatcga tcaatccttt tgtatcttct
    12241 gtcaacggca gcatgactac gatgtaatcc gaggtagaaa gaactgtttc taaatctgct
    12301 tgccgaacat aatccaactg ggcatcgcgg atagaataac ccaagacatc catccctaag
    12361 ctatagcaac ttagcgctac ttttgtgcct atcgctccaa gaccaagaag accgatggtt
    12421 ttgccttgca attctcgtcc cacataagca cttcttttgt tttctgcctg ttctaaaata
    12481 ttaggacctg ttagtgtttg taccatacga cttgcttcga ttatcggacg ggaagataag
    12541 agcaaacagc acaaaacaag ctcttttact gcattagcat ttacacctgg tgtattcatg
    12601 acgatcgtcc cgttttcaga agctttttca acgttgatcg tgttcacccc aacaccagct
    12661 cttgaaattg ctagcaaatc tggtgtaagc cattcatctt tcactttcgt actacgaata
    12721 actattcctt cggctggcaa atcatctatt attttgaaat attccccttg taacttttct
    12781 atctcttgtt ctggaatagc atcaatcaaa tgaacgtgat acataaaacc ccctccttac
    12841 taacaagtat ctcgaaattt ctgcaattag acaactgttt accatttgac ttcctagaag
    12901 caaaaagagc agttagagca gagtaggctg agtgataaaa ataaggctat gacaagttac
    12961 tgccataagc cttattttcg agagcaaaat attgagggtg agatatcttg ataaatggtg
    13021 ttccgttagc tgacattgat aataagacta aatttcaaaa aagttgaaaa gcaaatttcg
    13081 tcaatctctt cttatttctt aaaactaagt ggctttttca caacctcttt ttatctaata
    13141 accactgttt ccaactcaac taattttgcc cacgtttcaa tttgatctgt tgttagattt
    13201 agcgagacaa cggtatgatg accgccacct gcctgaatcc atgaatggac accttcatgg
    13261 aaattcggtt tagttttcca taacacacgt gcaacaggta aattaggtgc ttcagtcgtt
    13321 ggttcaaagg cttctacttc attgatcagc agtttataat gcgtaccgaa atctgccatc
    13381 gatacgacta ccccttctcc tgctttccca tcaaatacaa gacgcgccgg atcttctcta
    13441 tctcccattg ataaaggaga aacaacgatt cttggtttcg tcgcagcaag cgtcggatct
    13501 acttccatca tatgggattc caagattgct tcttggccac tagctagctc gtaagtataa
    13561 tcttccatga atcctgtatc tttccctcga gccatgattt tcagcaaccg atcaatcgct
    13621 gcagttttcc agtcgccttc cccagcaaaa ccgtatcctt ctgccatcaa acgttgtaca
    13681 gctagtccag gtagttgttt cattccatac aggtcttcaa aattagtcgt aaaagcgtta
    13741 tagcctcctt cttctaagaa acggcgaata ccgatttcct gctgagcttg tacttttaca
    13801 tgttcctccc atgtcttttc ttcataatca ccatagtcga aggtatacaa ttccttgtac
    13861 tcttcaaata attgatcgac ttcttcgtct gatacgcgat cgattacttg tacaagatct
    13921 ccgataccaa aataatcaac agtccaacca aattgaatct gtgcttccac tttgtctcct
    13981 tctgtgacac ccacgtttct catgttgtca ccaaaacgag ccacttttat tccaaaactt
    14041 tcattatagg ctactgcaac atccatccaa tcagcaatct ctttttggat ctcaggtcgt
    14101 ttccagtagc caacgacgat tttattttgt ttatttaatc gagcattgat gaacccatat
    14161 tcgcggtcac catgagcgga ttgatttaaa ttcatgaaat ccatatcgat cgttttccaa
    14221 ggaatgcttt cgttatattg cgtagctaaa tgaagcaatg gtttttgtag aagctttgtc
    14281 ccgcggatcc acatttttgc tggtgagaaa gtatgcatcc aagtgatcac acctgctact
    14341 tcttctcgat aattgacctc tttcatgatt tttgtgatcg tatcagcagt aactgctaat
    14401 tcttgaaggc ggattggata aggcaattgg ccattttcat tcagttcatc gaccatttct
    14461 tgtgcatgtt tctttacttc tcgcagtgct tcttccccat ataaatgctg tgatcctaca
    14521 acaaaccaaa attctttttc tccaatattc aacataatta gccactcctt ttatttgttt
    14581 tgtccgtaat acgcattttt tccgtgtttt cgtaaataat gtttatctaa aatccgttga
    14641 ggcagctctt ctgaaaatga attgagctgt ctggtaaata aattcatctt gcatacttca
    14701 tctagtacga ctgcgttcat taccgcactt tgcgcatctt tcccccaagt aaatggaccg
    14761 tgtccgtgta aaagtacccc tggaattgct aagggatcaa ttttgcgttc tttgaatgtt
    14821 tctatgataa cttttcctgt ttcatattcg tacccgctgt cgatttcctg ttgtgtcaaa
    14881 aatcgagcac aaggtaccga tccataaaaa gtatctgcat gcgtcgtccc catcgcagga
    14941 acatccaatc ctgcttgtgc ccagatcgtc gcccatgtcg agtgagtatg gacgattcct
    15001 ccgatttgag gaaatgaacg atataaaaca gcatgagtag gcgtatccga agagggattg
    15061 agtttacctt caatgatatg attgtccaag tccacaacga ccatgtcgtc tgctgtcatc
    15121 tcttcataac tgactccgct aggtttgata acgaaatatc gtgtttcagg atcaaaagcg
    15181 ctcacatttc cccatgtata tttgacgagt ccgtgtttag gcaagtcaag atttgcttgg
    15241 tatacttctt ctttcagttg ttccagcatt ttctatcact cctttttctt gcaattgatg
    15301 aatcgctgtt ttttcaatgt cgatcccctc acggtaacgt ctcataaata tctgatagcc
    15361 tgctatttct tctgctgaag gtgtgatggt gattccttgg tcttgtccaa acacgtcttc
    15421 tgccaagaag ttttctaaag acttcccatg tcgatccatc atataggctg ctaataacgc
    15481 gattccccac gctccacctt ctccggctgt gtccatcact gtaactggtg cctccatagc
    15541 agatgctaga atccgctgtc caacttctgg tgttttaaaa atccccccat gccccactaa
    15601 acgatcaatt tggacattct cagatttcaa tatatccatt ccgattctca ttgcaccaaa
    15661 agcactggct aaatggatcc gcatgaaatt cgctaaatca aaacggctac ctggtgtacg
    15721 tacaaataat ggccttcctt catttactcc tgtaatgttt tcaccggaaa aataaccata
    15781 agaaagtaat cccccgcaat ccgtatcgcc ttctagcgct ttgttgaata ataccgtgaa
    15841 taatttatct ctgcttatct ccatacccaa agaattggta aattcttcaa aaatactgac
    15901 ccaagcattg atatccgaag agcagttatt cgtatgaacc atgcctacta gttctcctga
    15961 aggtgtagtg accatgtcga tttccggatg gacattttct agttcttttt ctaatacgat
    16021 catagcgaag gcggaagtac cagccgaaat gttccctgtc cctttggcta cactgttcgt
    16081 agcgaccata ccggttcctg catccccttc tggcggacaa accgggattc ctggtctcaa
    16141 atttcccgta gggtcgatca gataagcccc tgattcgctt aacttgccag cttcttctcc
    16201 tgctactaga actttaggca aaatgtgatc caagtcaatc gctacgtgcg ctttttcgat
    16261 taactgacca aaaatatttt tcatttcttg atggtaggtt ttcgttgaag gatcgatagg
    16321 aaacatgcct gatgcatctc ctacgcctag tactttctca ccagtaagct gccaatggac
    16381 ataacctgca agcgttgtca aaaaattaat ttctttcaca tgttcttctt tattcaaaat
    16441 agattgatac aaatgagcga tactccatct ctgcggaata ttgtattgaa atttttccgt
    16501 taatattcgt tctgcttctt cagtgattgt atttcgccaa gtacgaaatg gtactagtaa
    16561 ctgttccttg ctatcaaatg ctagataacc gtgcatcatt gcactgaacc caatcgaccc
    16621 gattgtcgta agattctcgc catagatttc gaatacttct gctgccattt ttcggtaact
    16681 gacgcgcagt cctttccaaa tatcttctaa tgaataggtc cagataccat tttctagttt
    16741 gttttcccac tcataactac cagcggcaat aggagtaaaa gatgaatcaa tcaatacagc
    16801 tttgatccta gttgatccaa actcaatccc taaagatgta cgaccatcta tgacatctaa
    16861 catactttgc gcatttactt ccttcataat gatttaactc cctccttctc gtcaaacctg
    16921 atcaaaccgt ctttttagta gcgcttacaa tgattattgt atacttttgt acgtacatgt
    16981 caagcagtta tttacaagaa tacaataaaa aacacttgtt ttgtacgtac ataagttata
    17041 aaatgaaatt gcttacaaaa tctgatcaac cgccttctcg aaattctaga aggagagaat
    17101 acttatgaaa aaagaaaaaa aagtttcctc atcgtttatt tatttttttg gcgcatttgg
    17161 cggcattcta tttggatatg atattggtgt tatgaccggc gccttacctt ttttacagca
    17221 tgattggggt ttggcaggta aagcttcttt gataggatgg atcacgtctt cggtcatgtt
    17281 aggtgcgatc cttggcggct ctttatctgg tcttctttct gataagcttg gacgacgaaa
    17341 aatgattctt ctttctgcac ttatttttat ggctggttct gtcctttctg catctgcccc
    17401 tcacaatggt tcttattttc tgattgcagc aaggattctt ctaggtttag ctgttggcgc
    17461 tgcatctgcc ctagttcctg cttatatgtc tgaaatggct cctgctagac taaggggaag
    17521 attgtcaggg atcaatcaag tgatgattgc ttctggcatg ctcctttcct atgttgcaga
    17581 ttatttgcta aaagggttgc ctgaaacgat ggcttggcgc gtgatgttag ggctcgctgc
    17641 tgtccccgcg ctgattttat ttttcggtgt tttagctcta ccagagtcac cgcgttttct
    17701 tatgcaatct ggccgcttgg aagaggcaaa aagagtattg aactatattc ggacaccaaa
    17761 agaagctgaa caggaattcg aacaaatcca gctgaatgtc aaacaagaaa agacaaccgg
    17821 aacttcttgg catactttgt ttttagaaaa atatcgttcc ttagtcattg ccggtattgg
    17881 tgtagcagta ttccagcaat ttcaaggagc taatgctatt ttttactata ttccgctgat
    17941 cgtagaaaaa gcgactgggc atgcagcgag cgatgcattg atgtggccga tcatccaagg
    18001 aatcatttta gttgctggtt ctttgctctt tttggttatt gcagacaagt tcaatcggcg
    18061 aactttattg aaaataggcg gtagcgtgat gggactttct ttcatcctac cagctgtttt
    18121 aggaactgta ctggatgctc atacaaattc gttgttgatt ctcttgtttt tatgtattta
    18181 tgtcgcattt tattcttgca catgggctcc actgacatgg gtaatcgtag gcgaaatatt
    18241 cccactagct gtccgcgggc gagcatctgg cctagcctct tcttttaatt ggattggttc
    18301 tttcctagtt ggcttgcttt ttcctgtgat gactgcttct atgtctcaag aaatcgtttt
    18361 cggcattttt gggatcatct gctttttagg ggttttattt attcaagaga tcgtaccaga
    18421 aactcgtggg aaatctttag aggaaatcga acaaagtgca tctaaaaaaa cttatcctaa
    18481 acgtatttct atccatcatt cttaaagagg ttgtgacaga agtggtcagc ttcaagaaat
    18541 aagaaggaac cgcttagttt ccagtcacaa ggaacaattt caaaaaacag cttctcatgt
    18601 ttgatgaata ttgtgacttg tgaccagttg gtaggagaac accgtttagt ggagaggtgg
    18661 tgaaagaaac gttcagcctt gagcgatgac aaaagctctc acaaacattg cgacgagtaa
    18721 ccgtaggagc aataagaggc ggtaaaaacc cgtctcttat ttttcgtttt ctttcactgt
    18781 ttcccgttca actagtacag gttcatagag aatatttggc tgtgcctgtc ctttttcaat
    18841 cgttgtgacg atccaccgtg cggcatcttg tcccaacttt tcttttggat gcgtcaatgt
    18901 tgtcaatttt ttattcccta aatggcttaa agaagaatcg tctgttccta cgatcgatag
    18961 gtcatctgga atatgatagc cattttctgt caacttatct agcattaaac tagctatttg
    19021 atcgttatag caaatgatgc catctaaagc ttgttcttct ttcaaccgtt gtactgtttt
    19081 ttcagcgatt gtttctcgtg tttccgtggt gaaagtaatg atatcttccg tcaaaaaacg
    19141 cacattatac tgttcacacg cgctgataaa acctttcatc ctgtattttc cttgaagatc
    19201 gtcgattttt gtgatgaaca acagacgctg atgtcctttt tttatcaaat attctgctgc
    19261 cataaaaccg actttcacat catctaaaca aatataagag gcatttaact cttcataagc
    19321 agcattgatc atcaccatcg gaatgccttg ttctctcaaa gcaacataaa gtgctaagtt
    19381 tgggttatac tgattacttt tcgtaggctc aacgatcaaa ccatctaccc cataagcaat
    19441 cattttcttc aaacactctt tctccaattc gtgatcgtta ttcgtactgg ctaaaagcaa
    19501 tgaataccca gattctcgta attcattttc gattcctcgg ataatcgacg gaaaaatata
    19561 atcagagaga tatgtggtga tgacaccgat cgtttttgtt ctgttcaatg gtgttttttc
    19621 tttcttatag ctgtcatcta catatgtgcc cgaccctttt tctttacgca gatagccctc
    19681 atttaccaac acagcgattg cttgccgaat cgtatgtctg ctgacgtcat atatttcttg
    19741 cagcttgaat tctgagggaa tggttgtatt tacttcgtat tcctcacgaa ggatcttttg
    19801 ttttatctct tctgcaatat cttgatattt tgccatttgc cttgcccctt tttatttgta
    19861 cgtacatata tcataacgag tctgcaaggt ttattcaagc aaaaatcaag gtttcttgtg
    19921 ctcataaggc atacggaaca ggttcaccaa attctggaaa tccttgctca ttccacgtaa
    19981 agcattgcgc gttcgcgtgc cgattaggat tgtctaacgg atcccctttt tggttcttct
    20041 ctggtcgagc atgatagatc aaaatatcaa agcacccgtc ttctgaaacc gtaaacgagt
    20101 tgtgaccagg accaaattgc ttattttttt cagaagaaac gaatactggc tccgataatt
    20161 tatgccatga ttcttttaat aaaagattct tgtgctcatc agcccacagc aggcccatgg
    20221 catagttttc atctgttgca ctaccagaat acgtaatgaa tattttccca ttgcgagtaa
    20281 ggactgctgg accttcattt actgaaaaac cccgtttttc ccattcatac tcaggtatag
    20341 aaaggaggat ctgcttcccc tttaacgtcc aaggattatc catttcggat agataaagat
    20401 tagaattccc agtaatttca ggatcttgtt gggcccacac ataatatagc tttccttgag
    20461 aagagaaagt tgttccgtct aaactgaaag tttccctttt cgtttttatt tgtccctttt
    20521 ctatccaatc tgtattcatt ggatctatat gctgattttc aagtacgaac atgcggtgat
    20581 gatgagattc atcacgtata gttttatgat tgcttgcagc aaagtaaata taccattttc
    20641 cattgatata atggacttca ggtgcccaga tcaattcact catcggtcct tcttcatgag
    20701 cctgccatac aatcaggctt gcagcatgtt ctaattcatt cagtgacttt gcccttctta
    20761 attcaatcac ttggtagcct ggaaccgatc ctgtaaaata ataataacca tctgtatgtt
    20821 tatataccca aggatctgcc cgttgccgaa cgataggatt attataaagt gtcttcatca
    20881 taacaaattt cctcacattt tctttattct aattacattc caagagtatt tttctatccc
    20941 tacatttaat tcgtcatgtt tttctattga aaaatagttt atttcaggtt ttattttttc
    21001 tggttcttca cgtgtatttt tgtccaacag atcgctactg gttaatgtgt agtgctctcc
    21061 ttgatctagc aaattgaaat catgttcaat cgttatcttt tgttgttcat ttgtgcgatt
    21121 gatcaagaaa atagtcagct gattgccgtt atctacgatc acactgtcca cataaggaac
    21181 attttttatc ttttcacaat cataaacagg gcaaatgatt tgttgtttca atacttgacc
    21241 tttagcatat ttcgataagt gttgcatcac ataataagtt ggattcctcc atgcttgtcc
    21301 tttagcatct gtcagtatta aaggtattgt gtttactaac aatgattgac aactaatctt
    21361 tacgcgatct gcgtgtcgta aaatagccag ccctaatgaa ccaaataata atgtatcttc
    21421 catagtaaag taacaccaat cgatgggact accgacttca aaatccctat gtcttttttc
    21481 cggttgggca tgtacattcc attcatcaaa cgctagatac attgttttct cacttctcaa
    21541 atatcctttt acataatcac aagtagcaac gatttcttca atttgacgat cgaatcgata
    21601 agcttttgca aggtatgtcg ctgtatcatc acgctcattt ttaggaggtt tagtcaagtc
    21661 ttcttcatca tacttatcaa tatagttatg caaagctaaa taatctacat tctcataagc
    21721 ttcttctaat gcgatacgat cccattcagg gtacgatggt aaacgtggcg tagagcttcc
    21781 aactaagatt gtttcaattg aatcatccac taacttcatg acttttgagg cttcatttgc
    21841 taacctacca tattcataag cactcttagc ccccacttgc catggaccgt ctagctcatt
    21901 ccctaaacac cataatttaa cgccaaaagg ctttttttgc ccatttttta ttcttaaatc
    21961 actccagaag gttccttttg gaaaattaca atattctact aaattttttg cttcatcaat
    22021 tccttttgtt cctaaattca ccgtgaatat cggtttcgct tgtacatgac tgatccattt
    22081 aaaaaattcg tggagtccaa aagaatttgg ttctatacct cgccatgcta aatcaatttt
    22141 ttttggtcgt tgttctttcc tacctacagt atccacccaa tcataaccag atgtaaagtt
    22201 tcctccagga tagcgaatga cacctaattc taattccttt acataagaga ggacatcttg
    22261 tctaaacccg tcaccatcag ctgtttcatg gcccggttca taaatccctg tatagacgac
    22321 acttcccata tgctcaacaa atgagccaaa cagccgagaa ctgatctctc ctaccgtaaa
    22381 atcctctctg atttttactt tcacttcttc gtccaacagc tttccctacc tttcatttcc
    22441 tatagtcaag ataatagata caagtttcca tacgccacca gcaaagataa aaataaaagg
    22501 agggaaaaga aatatgagca gtacaaatcc taataacaat acgcaacaaa gaatatttct
    22561 caaaaaatca aaaaatactt tccatgcact ttgttcaaaa atttgtaaaa atgtgatatc
    22621 tttgttttca gaaatcacat atatccagga taaaagaaac atggaataaa taagtaaaac
    22681 tacatatcca ctaattgaaa gtacgattgt ctgttcttct aataattgat taacaagaaa
    22741 ataaataatg atcgttaaca gactaaggaa caagttctta ccaaaatgtt gctttattcc
    22801 ttgaaagaaa aaataaatga tatttcctga tccctcacct ttccattgcg ccattacata
    22861 aaaaacagca catgtagcag gcacgatagt aataacaggc aaacacacga ttacccagag
    22921 aatacctaat aagatgtaat cagaaaccac aatcaactgt ccataaagtt tagtctcaac
    22981 tattttcatc ttcctcactc tttcattcca gacatagctg ttccttgaac aaaatatttc
    23041 tgtgcaaaaa tataaaccac tagtaatggc agcattgaaa taaccgttcc agccattaca
    23101 ggtccataaa aagaaatgtt ctgccctgta aataaagcta accctgctgg taaagttctc
    23161 atttttgaac tacttgtcaa tatcaatgga taaagcaagt cattccaact attcatcagc
    23221 gtgaaaatcc ctaatgataa aagagtaggt ttgctcaatg gcaacatgat tttcaaaaaa
    23281 atattaaact cacttaatcc atcaatgcgt gctgcctctt ccaaagaatc tggtaaagta
    23341 ataaaaaagg aacgcatcat gaagatacca aatgcagttg tcatacgagg aatgatcaaa
    23401 cctgcataag tatcaagcat gccaagcaaa ttgacttcaa taaatagagg aatcatgaag
    23461 acttggaatg ggatcatcat tgtcagcaaa acgaaataga acaaaactga ttttcctttg
    23521 aaacgcatac gagcaaatgc ataccccgca agagaatcaa agaatacaga agtgatcact
    23581 gtccctccag caaaaatcac tgtatttttg atgtaatcta ataaagggat cgttgtccaa
    23641 accttaatat agttatctag ggtatagcta tctgcaaata ttctaggcgg aaatgagata
    23701 atgtcttttt cgtgtttaaa agaagaaata atcagccaca aaaaaggaaa cgcaataacc
    23761 aacaatataa ctagcatact aatcgttcga atgatttttt taccactaac tgcttttcga
    23821 ctattcacag taactgtaat cgagctttct ggtgccatac aggtttccct cctatttcat
    23881 ttgtttttct ttccgtgaca tgtacacatt agtggagata gctatagttg caacaatgat
    23941 aaacaaaacc gaagacagtg ctgatgcata gcccaattca taaggtgctg taaatcctcg
    24001 actgtaaata tattgaacag ctgtttctgt cttaaattgt gggccaccag cggttgcaac
    24061 atatacttgg tcaaacactt gcatcgaacc aatcagattc gttaaaatac aaaaacctaa
    24121 ggagggaacg atctgaggta gcgtaatgtt aaaaaactgc tgtcgatttg ttgcaccatc
    24181 catttgagca gattcataca aagagggaga gattccctga atagcagtca agagaatcgt
    24241 catagtaaca ccaaaattct tccaaacagt catgatggct accgtcggca tagcaagggt
    24301 tggatcgcga aaaaattgag ggatcggcaa gccgacttgt gaaaacaagt aaggaatcgc
    24361 ccctacattc ggatcaagca acatagaaaa aataatcgca attgacgtca atgaacaaat
    24421 aactggaatg taaaatgtcg aacgacaaaa acgattgaat ctggtatttt tagataatgc
    24481 tactgctgct aataaaccaa cgataatttg tgttggcgtt tcgagtacgg tgaaatataa
    24541 agaatgaatc aacgaattaa ctgctcgttc atcactaaat atacgtgtga aattttcaaa
    24601 acttacaaaa ttt
//
LOCUS       DAKBVG010000012        24426 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000012 DAKBVG010000000
VERSION     DAKBVG010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 24426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.012"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(361..1479)
                     /locus_tag="OUD09_000416"
     CDS             complement(361..1479)
                     /locus_tag="OUD09_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9691721.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1950..3257
                     /locus_tag="OUD09_000417"
     CDS             1950..3257
                     /locus_tag="OUD09_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HCT9691722.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3376..4575
                     /locus_tag="OUD09_000418"
     CDS             3376..4575
                     /locus_tag="OUD09_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCT9691723.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4602..5870)
                     /locus_tag="OUD09_000419"
     CDS             complement(4602..5870)
                     /locus_tag="OUD09_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HCT9691724.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(6033..6679)
                     /locus_tag="OUD09_000420"
                     /pseudo
     CDS             complement(6033..6679)
                     /locus_tag="OUD09_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
     gene            complement(7087..7590)
                     /locus_tag="OUD09_000421"
     CDS             complement(7087..7590)
                     /locus_tag="OUD09_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCT9691725.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(7713..8477)
                     /locus_tag="OUD09_000422"
     CDS             complement(7713..8477)
                     /locus_tag="OUD09_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HCT9691726.1"
                     /translation="MKEIQSVKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLAEVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSSTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            8584..8859
                     /locus_tag="OUD09_000423"
     CDS             8584..8859
                     /locus_tag="OUD09_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCT9691727.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            9575..10525
                     /gene="yidC"
                     /locus_tag="OUD09_000424"
     CDS             9575..10525
                     /gene="yidC"
                     /locus_tag="OUD09_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCT9691728.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGGAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVIMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPADNKQATKQIKQPKNSTNNKGRNAGRQQKRK"
     gene            10629..11856
                     /locus_tag="OUD09_000425"
                     /pseudo
     CDS             10629..11856
                     /locus_tag="OUD09_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012008915.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
     gene            complement(12166..12387)
                     /locus_tag="OUD09_000426"
     CDS             complement(12166..12387)
                     /locus_tag="OUD09_000426"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCT9691729.1"
                     /translation="MFKNYTINQKILPLDMEQYISETDVAFAVNALVEEKIKVDLYVS
                     SKFTNVKVVGADLFVVNVRKQKVSETDKF"
     gene            complement(12548..12946)
                     /gene="tnpA"
                     /locus_tag="OUD09_000427"
                     /pseudo
     CDS             complement(12548..12946)
                     /gene="tnpA"
                     /locus_tag="OUD09_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513861.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(13160..13526)
                     /locus_tag="OUD09_000428"
                     /pseudo
     CDS             complement(13160..13526)
                     /locus_tag="OUD09_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387104.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
     gene            complement(13540..13825)
                     /locus_tag="OUD09_000429"
                     /pseudo
     CDS             complement(13540..13825)
                     /locus_tag="OUD09_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
     gene            complement(13831..14025)
                     /locus_tag="OUD09_000430"
     CDS             complement(13831..14025)
                     /locus_tag="OUD09_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9691730.1"
                     /translation="MAETRPKGYPKLKEYTPSRFMLSECHYDKARADRAVNFIGQLRH
                     TKGKWAGNRFWLLPWQEQII"
     gene            complement(14025..14501)
                     /locus_tag="OUD09_000431"
                     /pseudo
     CDS             complement(14025..14501)
                     /locus_tag="OUD09_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290865.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
     gene            complement(14969..15322)
                     /locus_tag="OUD09_000432"
     CDS             complement(14969..15322)
                     /locus_tag="OUD09_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9691731.1"
                     /translation="MAMSEKAKKYLKEIKGAKTIPELKDVEIAIKRDGILAWAEFTKL
                     NEAVEEKKVALRKKKQETSLQEILFWAYKKESDKLLKMMDEGADKDAIQMQVQRYDSI
                     GQIIAEADLEDEYEV"
     gene            complement(15450..15989)
                     /locus_tag="OUD09_000433"
     CDS             complement(15450..15989)
                     /locus_tag="OUD09_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9691732.1"
                     /translation="MQLLLYRIGDSTKIRFLINVRFRVIYSTVLPKGIPQRQKWMIHM
                     EEIMQMILGNAKFQLGQVVSTPAVLETFNPLQTANGIPEALSNLWFRQEISIAEIEDA
                     VLDAKNGKELANSLNRIKLFRKFTLDRELDGMVRLKGTDVWKNISYLKVYTATPPKKP
                     TPPRFQSNGWSLFFSHKSA"
     gene            16019..16210
                     /locus_tag="OUD09_000434"
     CDS             16019..16210
                     /locus_tag="OUD09_000434"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9691733.1"
                     /translation="MIIRDDHIYTCDSCHYSFPADEQPERCPDCEKTATRLDTEIETE
                     DYYRVRAEIKAEIKALNAG"
     gene            complement(16252..17118)
                     /locus_tag="OUD09_000435"
     CDS             complement(16252..17118)
                     /locus_tag="OUD09_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCT9691734.1"
                     /translation="MEELFSNALKSVKSNRVLYTASFFARSSLLYLQEIGQLTALKAH
                     TSRRTGLSSYLCFLVEVGSGTLEYKGQKYTLQVGDMVFIDCLKPYVHESSEDDLWTLR
                     WCHFNGIGMTEVYKKYLERGGQPVFKTSKTDKYADLLRSLHMVAEGQEYVRDMKINVL
                     LSELLMHLMEDSWNPDKVPNFSTKKMELQNIKQYLDDHYTEKITLDQLSAEFFINKQY
                     LAKIFRETYGTTVVGYLNQKRITQAKHLLRFTNLSMEEIATKIGITDANYFSRTFFKV
                     EGVRPSEFRKMW"
     gene            complement(17142..18794)
                     /locus_tag="OUD09_000436"
     CDS             complement(17142..18794)
                     /locus_tag="OUD09_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HCT9691735.1"
                     /translation="MNYKVEYERWLNHANADIIEELRSYSEKDIEDSFYKNLSFGTGG
                     LRGTIGAGTNRMNVHTVGKASQGLADYLNKNYEAASVVVGYDSRIKSDVFAKTAADIF
                     AANGIQVHLWPELLPVPTVSFATRYLKADAGVMITASHNPSKYNGYKVYGADGCQITT
                     EAATAILAEIEKLDIFADVKMDGDASKIASIQSEVLTAFIEEVKGQSVLFGEEVDKNV
                     AIVYSPLNGTGLVPVTRTLAEMGYSNVTVVEEQRLPDGNFPTCPYPNPEIKEAMSLGM
                     EYAKKYNADLLLATDPDCDRVGIAVKSAHGDYVLLSGNETGLLLLDYICSQRIKHGKM
                     PADPVMVKTIVTMDLGERIASNYGVRTIDVLTGFKFIGEQIGMLEKAGKVDSYIFGFE
                     ESYGYLSGSYVRDKDGVNAAYLICEMFSYYKTQGISLLDKLNEIYTKYGYCLNTLHSF
                     EFDGAAGFTKMQEIMVEFHKGLDIIGGKKVIETLDYSKGLNGLPKSDVLKFMLEDNCS
                     VVVRPSGTEPKLKTYISVSAENKEAAEAMEAIMAAELAEMFE"
     gene            complement(18799..19701)
                     /locus_tag="OUD09_000437"
     CDS             complement(18799..19701)
                     /locus_tag="OUD09_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HCT9691736.1"
                     /translation="MMNLQKELRCLLLRKLGMLYAPGWTELCCVWSEYQGMTLTEVLQ
                     EHPEFLGTHPQTKGELPILIKFIDAKKDLSVQVHPTDEYAIEKENGQLGKTEMWYVLD
                     ASLIYGLQKITEEAVIREAIAQGTVERYLQKVPVKKNDVFFIEAGTIHAIGAGALIAE
                     IQENSNLTYRLYDYDRVDKNGKKRELHVDKALEVANLKGLSEPKQPLRVLKYRAGVAA
                     ELLCRCKHFEVSRMIVNTERRQTVHYRADSISFRVLLCVNGCGTVRFDNESLDIYKGD
                     CLFVPADSTEISIHGSIQFLDVRG"
     gene            complement(19785..21098)
                     /locus_tag="OUD09_000438"
     CDS             complement(19785..21098)
                     /locus_tag="OUD09_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HCT9691737.1"
                     /translation="MNIVLLSGGSGKRLWPLSNDIRSKQFIKIFKTEDGYESMVQRVY
                     RQIKKVDNDATVTIATSKTQVSAIHNQLGEDVGVSVEPCRRDTFPAIALATAYLVDVM
                     GIDPEESVVVCPVDPYVEDDYFEALKSLSDQADKGEANLVLMGIEPTYPSEKYGYIIP
                     ESAEQTAFVNTFKEKPSEAVAKEYIAQGALWNGGVFVYKLKYVMERAHQLIDFVDYQD
                     LFNKYDTLTKISFDYAVVEHEDKIQVQRFAGMWKDLGTWNTLTEGMEESIIGKGVMNE
                     NCDGVHILNELDVPVLAMGLHDVVICASREGILVSDKEQSSYIKPFVDKFEQQIMFAE
                     KSWGSYKVMDVEAESMTIKVTLNASHSMNYHSHKNRDEVWVVLAGTGKTIVDGYEQVV
                     KAGDVITMSAGCRHTIMADTELKLIEVQLGKDINVKDKQKFELEY"
     gene            complement(21166..22110)
                     /locus_tag="OUD09_000439"
     CDS             complement(21166..22110)
                     /locus_tag="OUD09_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014424865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HCT9691738.1"
                     /translation="MMEKDAKIYVAGHRGMAGSAIVRELNRQDYNNIITRTHKELDLC
                     RQDAVEAFFAQEKPDYVFLAAAKVGGIIANQNALADFMYENMILEMNVINSAWENGCK
                     KLQFLGSSCIYPRMAPQPMPESCLLTSELEKTNEAYALAKISGLKYCEFLNKQYGTDY
                     ISVMPTNLYGPNDNYHPTHSHVLPALIRRFHEAKEAGLPTVTCWGDGSPLREFLYVDD
                     LANLCVFLMNNYSGDETVNAGTGKELSIKELTEMVAKVIGYEGEILWDTSKPNGTPRK
                     LLDVSKATKLGWTYKTELEDGIRLSYEDFLNNPMRAER"
     gene            complement(22147..23160)
                     /locus_tag="OUD09_000440"
     CDS             complement(22147..23160)
                     /locus_tag="OUD09_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9691739.1"
                     /translation="MATLCRCEAKITLVCGANHLDFAKRNLEQELPKELIDRVRYRPI
                     NTDIGLINREGTLLVDTGKLTEICEYFLAKVPSLAKTEAEWLKREAVSAVLCDMPIWA
                     MDACDMAGVPFLYVGNFTWTELYREFLPEHIWKEYAKRYGKIQHTMLYALNNEEMLEF
                     LQNSEKTPTSVVARPIHNDEVQRIKSQHEKPPVFVALGMSARFKQPIDVSHAPYDFIT
                     TLGVPLVGENVTVISNSTPNTQDYIAAADYVITKAGWGTVAECILAQRPMALFARDTV
                     LEDRTTICKLQKLGLAISVTPENLHRVERVIEDLKQLDTSNFDTFYDAASEIADKLIS
                     LMQ"
     gene            complement(23240..24286)
                     /gene="gmd"
                     /locus_tag="OUD09_000441"
     CDS             complement(23240..24286)
                     /gene="gmd"
                     /locus_tag="OUD09_000441"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HCT9691740.1"
                     /translation="MKKALITGITGQDGSYLADFLLEKGYEVHGITRRASISNTARID
                     HLMGKITLHDGDLSDSSSLIRIISIVQPDEIYNLAAQSHVQVSFDVPEYSGDVDALGV
                     MRILEACRILGLTKKTKIYQASTSELYGKVEEVPQRETTPFHPYSPYAVAKQYGFWIT
                     KEYREAYGMFAVNGILFNHESERRGENFVTRKITLAAGRIAEGLQDHLELGNMDSLRD
                     WGYAKDYVECMWMILQHETPEDFVIATGEQHTVRDFTEKAFAANGMTIRWEGTGIDEK
                     GYDVETGKMLVCVNPQWFRPTDVDNLWGDPTKAKTVLGWNPQKTSYEELVNIMAKHDR
                     ELAKREAAMKNAVK"
BASE COUNT         6946 a         5537 c         4651 g         7292 t
ORIGIN      
        1 acggtgttcc agaagtagct tcttcggaaa taagccgaaa tttcacaaaa atttgagaaa
       61 taatttttgt caatctcttc ttatttcttg aagctgacca cttctgtcac aacctcttcc
      121 ttaaacggtg ttcaaaagct tgcatctgcg gtaataagcc caccaaacaa aaaatatgaa
      181 gagctatttt tgcttgctgt tcttattact tgatgcagaa cagctttttc acaacctctc
      241 atacacggtg tttaatcagc tgaccttcgg tattagctca aaaaacggaa aaatatgtga
      301 aactattttt ccgtttgttt ctctaatact caggtcaaaa cactgatttc acaacctctt
      361 ttaataacga agaatcgctg tcagactctt ttcatctggc gcatcttttt gatccaaaat
      421 aaacactttc ccgccattaa tgatcacatc atctgctaat gcattcaaca cttgacgccg
      481 atcatattct gtttcaggat cttcaccgaa accatcagct aagtttgacg tagcgataaa
      541 taaatgtgac acttttcctt ctttcgcagc tggaacgata tcaactaact gatcgatgaa
      601 tttcttatct aagagtttat tgtattcttt tgattcttga gctgcgagtt cacttgacag
      661 cttcttcgcc ccttcttcga tatctctaaa ggatagctgc gcaggagaag cagaaatcgc
      721 aacgtctgca caataatagg gatttttagc aatttttttg aacatcgtct gattttctgg
      781 taatgcaaat aaataaatcg gccattgttc aggattttcg aaagaatcct tcaagtacgt
      841 atcaacagct tggtaatagt tgacccaatc gatctctact tcttcatctt ttggatttac
      901 gccatggaaa gttactccat ctcgggctcc gcctcctcca ttagaagaga agttcaaacc
      961 gccacctgtc agttcagatc ccaatgcagt aacgacatca accggtgccc cttctggtag
     1021 agatacttct tttacttgtt tgttgctgac ctcatacaat ttcatggagt cgcggttcaa
     1081 tcccaataaa taataagaat agttgaactg cccatttttg ataatagcca gtaaataagg
     1141 aagatcactt acatagtatt gatcgtctac cgggatactc aaacgatgga tataggtctg
     1201 gtcagcagtt ataatgaccg ctacactcgt agttgcacta cgccaaaacg aaggatctcc
     1261 taacaaattt tcaatttttt cttggaacgg ggaccagtca tgttcggaat attttttctc
     1321 aaaacgtttc tttgcttctt tcgcaaaatt tttgaatttg atctgatctt tttccacatc
     1381 ttgatgggcg acatgcgtat tcagcatgat cgtgacgaat ggtcctttag cgccttctga
     1441 agtcaattgt gtcaataatt ctttatcctt ttttgccata aacgtacccc tcctaaaaaa
     1501 atcattcctt tgtttctatc taaagtctac cacaacacta tttttccata caataaaacg
     1561 tatctaaaaa aataaaacgg tttctacggt tttaaattta gctatttttt taagttatct
     1621 tcataatttc ctcaattatt tagccccagt cttcaactat tcttcatgaa atgctgataa
     1681 actaagaaaa agaataaaag aggtggatct tatgcatttg attatgattt tcagtttatt
     1741 aagtattatc agtttatttg ctttcagcgc ttcccgctta ttagatgcgg aaacacagcc
     1801 tattcctgta aaagtacgta aaaacgaccg ctaaacttcc ataacgcgcg ttcttatcct
     1861 aaagtttttt cagaaacatt gaaactctct cccctctctg ataaaatttt aatttgtagc
     1921 gaaacaaatt aattacaagg agatcggata tgtccataaa aaaaacacgt ctagtcctat
     1981 tattggctag tctcattctt tgccttgctt cttttctacc agctgttgct gtgcaagctg
     2041 aagatacgtt caaagtaaat gcaaaagcag cttttgctgt agatgcgcaa agcggtaaga
     2101 ttctttatga tcaagatgga gaaaaaacca tggggatcgc ttcaatcaca aaaatcatcg
     2161 gtctttacat cgtccttgat caagtaaaag aaggaaaact gtcatggggt gataaagttt
     2221 cgatttcaga ttatgcagaa aatttgagta tcaccccaga tttatcgaat gttcctttgc
     2281 ataaagaaaa cacctacact gtcaaagaat tattcgactc tgcaatcatc cagtcagcca
     2341 atgcttcaat ggtggccttg gcagaaaaaa tctctggcag cgaagctaaa ttcactgaac
     2401 gaatgaaaga acaattgaaa gattggggaa tcaaagatgc aacaatcgtt aatgcatcag
     2461 gattgaacaa ctcttatctt ggcgaaaacc gaccagaagg cactggagaa aacgacgaaa
     2521 atcaaatgtc tgctcaagat gtggccatcg ttgcccgcca tttgatcttg gatttcccag
     2581 aaatactgga tgtatcaagt actacgaccc aaatgtttgg cgaaaatacg caatcaccag
     2641 tggaaatggt caactggaac tggatgctcc caggattcgt taattacaag gaaggcgtgg
     2701 acggattaaa aactggtacg actgagcttg caggtgcttg tttcgttggt acgatcacta
     2761 aaaacgatca acggatcatc actgtcgtat tgaatgcgga cggccatgcg gaaaacccga
     2821 gtgcccgctt caatgaaaca gaccgtttga tggactactg ctatgacaat tggtcagaaa
     2881 aagagctcgg aaaagctaac gcaagtattc ctagtctaaa aaccattgat gtaaaagacg
     2941 gaaaagaatc aacagttcct gttgtgctta aaagccctgt aaaagtatgg gtacgaaacg
     3001 atatggatac tggcaaatta acgatcacgc caacacttga caagaagcaa gtcactgaca
     3061 atgagatcca agcacctatg gataaaggta caaaaatcgg taccgctacg atcaccttgg
     3121 cagacgataa attaggctat ttagaagaca acaatgcacc atcaacagag atcattacgg
     3181 ataaaacagt ggaaaaagcg aatatattcg tgattggctg gcgacgcgtc gcagaatttt
     3241 tcggcaactt gttttaattg aacaataaga aaaacatctt tccatcggaa agatgttttt
     3301 ttgctttttc tagtttaaat aacagctttt ttcatctatt ttcgttatac tgtataagaa
     3361 aaaggagttg aatttatgaa tctaaccaga gatctgatct ttttagttct ctatcttatt
     3421 ggggtattct ctatcgttta ttggcgaaaa cagaagaagg atccttcctt gttcttttac
     3481 ggcagtacgt ggacatttac ggtaatctta acttattttg tcgtgttgga aattttttat
     3541 tcgacctttt attttttgat tccattgttg ttctttagta tctttgcttt ttcttatttc
     3601 acagaaaaac gaaggctact gaatggtctt ttattcaatg ttttactcat cagtttcggc
     3661 atctacttgt ttgtcttgct gtatgaaacg caaaatattt ttcttggcgg actgatcgcc
     3721 ttgatcacga ttccattgct acttgtcctt ttatttggta tttatggatt gattgttttt
     3781 cttttttgga acggggtcac agttttacgt agagaatcgc attcattagc taatctttta
     3841 acgttgatac tagccatttt tcttacactg tttcttgttt ttgacttttt cttgttaaaa
     3901 tatttgccgc aatggataaa tgctttgttt ttctgcgttc ccctgatctt gatctatcta
     3961 tttatcgtat tctacaactt tttgactgtc tcattcttgt atcagttcaa ccggcctcga
     4021 tacaatcaag acttcatcgt tgtgttaggc gctggattga tcaatggaga aacagtttct
     4081 cctcttctag ccaagcgaat caataaggca attgcttttt atcgggctca aagccgtgct
     4141 acattaaatc cgcccatctt attgatgtct ggcgggcaag gagcagacga aaaagtacca
     4201 gaggccattg cgatgaaaca atatgccatg gaacaaggaa tccctgaaag agacattctt
     4261 gtcgaaacca attcgacaac cactttggaa aacatgctct attcaaagga aatcatggat
     4321 cagcaaatga aagggccata ccgcgcgatc tttagttcca acaattacca catttttaga
     4381 gccggtcttt atgcgcggca agccaaattg aaagcaaatg gtattggtgc aaaaacagct
     4441 ttctactact tgcctaatgc gtttttaaga gaatacctcg ccatcttgat gatgaacaaa
     4501 aaaagacata tgatcatttg cggattgctt ttcgcaggct ccgcattctt atcgctgatt
     4561 actttacttt tctaaaaaaa caaggtgtga caaaacatat gtcacacctt gtttttttct
     4621 ttcattctct taactattgg caaaaagagt atcccttttt tgaccgctac ttgctgagca
     4681 gaataaatac cgctgttgcc tgaacacata aagctgatca agcagatcat aaagaagtat
     4741 actgccgctt gtgaaccgaa caattcgatc cccatcacaa agcaggcaat cggtgtattt
     4801 gtcgcaccag aaaatactcc gataaagccc aatcctgcaa gaaaaggaac agaaacatgg
     4861 agcaaaggcg ctagtgctgc accaagcgtt gcaccaattt caaacaatgg tgtcacttct
     4921 cccccttgat atcccgcgcc taaagacaaa actgtgaaga ataacttccc aataaaatca
     4981 tagacatgtg cctgtccatt aaatgattct tgaagtaacg gaagactcaa tccgagataa
     5041 cgttgtgttt gcaaaatcaa tgctgctgcc acaactacag ctccacctat cgtatttcga
     5101 caaacaggat gaggaatcca ttgtgcatat cgcgctttta aaaagacgat cgaacggctg
     5161 aatacccaac caatcaaacc aaaagcaatc ccagcaacaa aaactttgaa gaacagcaac
     5221 atactccatt caggaatcac acccatttgg taatgtagat gagaaacacc atagctttct
     5281 gtcatgaaat ttgcaaacag tgcagcaaag aagctaggga agatcgcttc tgttcggact
     5341 ttcccgattg ccaacacttc taatccaaat aatgtcccag ctaaaggtgt gccaaataca
     5401 gaactaaaac ccgcactgat tccactgata atcaagattt cccgttctga tttatccaat
     5461 cggaacaaac gtgccgtatt atctgctaac gctcccccca tttggactgc tgttccttca
     5521 cggccaacag atcccccaaa taaatgggta gttatggttc caaacagtgt aagtggaata
     5581 agccgcaacg ggattttttc ttctccacca tttccttgat cgatcactaa gttgtttcca
     5641 cgactagaaa gtccgccgtg atacgcatag agataagcaa aaagcgcacc actaactggt
     5701 agtagacaaa ggagccaagg atgtgatagt cggatatctg tcacaactgt caggctattt
     5761 aagaaaaaag cagacaagct ccccatgaat ataccgagaa tcgctgtgat caccagccac
     5821 ttcagcatat acactaaaat gcgtactgga ctcggtaatt gtttttccat aaaaaattcc
     5881 tcctcaataa aaaaacgcct actaaagaaa ggggctgtcc ccagacttta gtaggcgtca
     5941 ttagctatct ttcgttagca tttaaaggcg aacaccatca cctattcact tccattaaaa
     6001 gatagcatat ttttctgtaa aaaacaataa aattatcgtt ctttctttac acgttcagca
     6061 aattcataga aaggagccaa tttcgttaac tgttgattac attggtcact atcatgacaa
     6121 atataattcc cctttttcgt atacgtgcca tcagaacctg ctttcgttgt agaaagaaaa
     6181 agcgaaacat tcgatgtttt ctgacagatt gcacagattc ccttatttat cgttggtgat
     6241 aaagtaccgc taatcccttt taatttttca tcttcgtaat agaccatgaa ttttttctgc
     6301 gtacctggat cattccagcc gatgtacgta tgttcttgca agtcgatttc attccaagct
     6361 ggttgtttga gttttttcac ttttcggaat aatttctcga tttgtggttt gctaggctgc
     6421 ataaacggct gaacgatttg cttcagctcc tccaatgctc tttctgcttg cgcttttgtc
     6481 agttttacgt ctaaaactgt cgttaaaaat gtatcgattc tggcagttcg tgatctaata
     6541 tgtctcgaat cttttcagct gctagtgcct gaactgtttg taccgttttt ggatcattga
     6601 cagatgagta cgcatttaat aactgttcta cttctcttat tataaaaaaa tattggtatg
     6661 gttttattgt ttgttccatg attttaaagt cctttcttct aaccaaagtg catactttgt
     6721 agaaaaaagt gatcttctgc aaagtatgtg ttacctgaag atcctggact cttttaaata
     6781 agatacgttt tgcatcttat catcaccccc tttaatgctt attattctat gtacatgatt
     6841 cattcgataa ataaaagtaa accgtatctt gaacagatag tcaactgcaa acagaaaaaa
     6901 ggactgtgac aaaagtcgtg tcaaagtccc ttgtttcgaa tgaatggtgt tcaaaagctt
     6961 gcatctgcgg taataagccg aaacttcaca aaaatttgag aagtaatttt cgtaaattcc
     7021 ttcttatttc ttgaagctga ccacttctgt cacaacctca atatggtcta ttattggttt
     7081 tacctgttaa atcgaggatt ctttcccaaa gtaacgttgc catctcttac gcatacttgc
     7141 agacatgggc tgagccgctt ctttgactgc gcagtatttg tccacgaatg tcacaatata
     7201 tccttcttta tacttaggcg gtgcgatcgt tgcaccccac atgtgcttca aaatgatatc
     7261 tcgctcaaga tccgacaaat cggtgatttt ttctgcattc tttacagcaa ttcttggatg
     7321 gatatacgca tgtgtcccct cgtcaaattt cgtcgtacgc caatcatagt agaacagatc
     7381 atgcaaaagt ccagcacgcg ctgttgcttt agcattgcca ccccattttt ttgcgagcag
     7441 ataactatag taagaaacac tgatggaatg ctccaaacga gtggaatgaa catgctgtgt
     7501 gtaattcgct aattttttca ctgcttctgt ttctaaaaga tcttgtacat aagacatata
     7561 ttcttcgtct tcacgccatt tttctgtcat aattatcgat ttgccacctt tttgaatttc
     7621 attttcttct gaaattgatc taagtatacc accagtgttc ggattattct agtcgctcta
     7681 gataaaatag ctaaagctta atagttcctt aatcataaag aaaaatgata aagaacgatc
     7741 cctgctgcga ctccgacatt caatgattcc gcatttcctt tcattgggat atatacgttc
     7801 tgatcagtcg aagacaagat ttcctgactt actccctgac cttcgtttcc catgattata
     7861 gcgaaatcct ctgttttttc tactttgtct aatgcatgtg cttcttcatt taattctgta
     7921 ccgtacaccg taaacccttt gcctttcatt acagggatca cttctgccaa atctttttgt
     7981 aacacaggca aatgataatt gcttccctgc atgcttcgca gcacctttgt actataaata
     8041 tctgccgttc ctttacctaa taaaacacca gccataccaa acgcatctgc cgtacggatc
     8101 atcgttccca catttccagg atcctgtaca ttatctagta aaagccagcc tcctgaaaaa
     8161 tctggcgtat cttcagatgt taacgtcatc actgctaaga tcccttgcgg tgtaggtaga
     8221 tctgacatag cggtcatgac ctcatctgaa actagaaaag tagaaatctc tttgtttaca
     8281 gccagccagt ctccccattc tttcatacct ctctgtgtca caaaaagttc ttttatttct
     8341 gcatcagacg acgcagcttc ttcgatcaga tggaaccctt ccaaaagata ttctgccttt
     8401 tcttctctgt atttcttttt atgtagtttt tttaattctt tgattcttgg attttttact
     8461 gattggatct ctttcacatt cctcacctca actgattata tcatggaata ggtgcttgag
     8521 cttgtttcat cctaaaaaaa tcggtatcat tgatttataa aacaggaaag aaaggtgacg
     8581 aacatgcgca aagttaaaat gaatgtccaa ggacgtgtcc agggcgtggg atttcgctat
     8641 atgaccaaga tggttgctga tcagttaggc gtgaccggaa cggtaaaaaa cgaagacgac
     8701 ggctcagtca caatcgaagc aatcggaaat gacgacatca tccaaaaatt tatcgaagaa
     8761 gtcaagaagt ctccgagccc aagtggacga gtgcagtatg tggatgtcca agaagatccg
     8821 atgattgagg aacgaaagaa atttgatgtg gtgggataag gttgtttttg aagcgctttg
     8881 cctcgagtat taaggagaaa cattttccaa atagcttctc atattttgaa atgtaatgac
     8941 ttaataccga gaggcagctg ataaaacacc gtcaataagg ttgtgaaaaa gctggtctgc
     9001 atcaagcatt aaggactggt tgtgaaaaat agctctccat atttttcaac aggctggact
     9061 taatgccgta gatgcaagct tttgaacatc gtcgataagg ttgcgaaaaa gctgatctgc
     9121 atcaagcatt aaggactggt tgtgaaaaag tcgcttagtt ttgagtatta aggaaaattt
     9181 tgtgaaaatg gctttccaca tttttgcata aatttgactt aataccaaga aactgacttt
     9241 tgaacatcgt cgataaggtt gtttttgagg cactttgcct caagtattaa agacaggtag
     9301 taaaaaagct gatctataac ttgcaataaa tttgcttaac ccatattttc cagacagaac
     9361 aaaaaagttt ttagtttttt aaataagaaa gaaacacttg gaatcactct tttctttcta
     9421 agtatttctt tcttgatttt ttgtcttttt tcaaaatttt tcgctacgaa aaagaacgaa
     9481 tatttatatg aaatttctat gaagttgatt gaattccatg cgctttttta gtatagttaa
     9541 gtttgtgtaa aaattataga agaggaagta ttctatgaat aaatggaaaa actggttatt
     9601 gggatccgga cttgtcagta tcctgctctt tttatctggc tgtgtgcgga tggattcaaa
     9661 cggaaaccct gacacttcag gaatcgttta tcgagtactc gtccatccta tgggccaagc
     9721 gattacatat ctagttgaga atttcaattg gtcttacggc tgggcagtta ttgcaatgac
     9781 tgtgatcgtt cgtatcatca tcttgccact aggtatcagt caatcgaaaa aaacaatgat
     9841 ccaatctgaa aaaatgcaag cgttaaaacc acaagttgaa gcagcacaac aaaagctaaa
     9901 agctgcaaca acacgtgaag aacaaatggc agcacaagca gaaatgcagc aagtatatcg
     9961 cgaaaatgga ttgagtatga ccggcggaat cggctgtctg cctctattga tccaaatgcc
    10021 gatcttctct gccttgtatt ttacggctcg ctacacagaa ggtatccgtg aatcatcttt
    10081 ttacggcatc gatctaggct caccaagtat ggtgcttgtg gccatcgcag ggggcgctta
    10141 tctgcttcaa ggatatattt caacaatcgg tattcctgaa gaacagaaaa agacgatgcg
    10201 tacgatgctg atcgtctctc ctgctatgat cgtcttcatg tcgatcagtg cgccagccgg
    10261 tgttacactt tactgggtag tcggtggtgt gttcagctgt ctgcaaacat tcatcacaaa
    10321 cgttataatg aaaccgcgtt tgaaagcaca agtggcagaa gagttgaaac aaaacccgcc
    10381 aaaacaagtc gttactccaa gaaaagacgt gacagaatca gcaaagccag ctgacaacaa
    10441 acaagcaaca aaacaaatca aacaaccaaa aaacagtaca aacaacaaag gccgcaacgc
    10501 aggaaggcag cagaagcgga aataaaaata tatgacaaaa aactgtagac aaataaaaaa
    10561 atttctttgt tctacagttt ttttgttaca attaaagaaa acgttcgcat ttcgaggagg
    10621 aatgtataat gatgtccaaa taatcaatgt atagtcgaat ggaagaagac aaaatttcgc
    10681 tagatgcact tgttcctaaa aatcatttag tccgtaaaat tgacaaatta ttgtcatttg
    10741 attttattta tctcattgtt gaatcaactt actcgaccat tggtcgccct agcgttgatc
    10801 ctgtcgtctt agtaaaatta gtattcattc aatatttatt tggaatttgt tcgatgcgtc
    10861 agaccattaa ataagttgaa acaaacctag cttatcgttg gtttcttggg cttagtttaa
    10921 ttgataaagt tcctcatttt tcaacttttg gtaaaaacta tgcacggcgt ttcagagaaa
    10981 ccgatttgtt tgaacagata tttatcatta ttctcgaata agcagttgat gctagattta
    11041 ttcatgaaga aattttgtat atggattcaa ctcatattaa agcgaatgcg aacaaacgta
    11101 tttttacaag agatatggca cataaagaag ccattttata ccaaaaacaa ttagaagaag
    11161 aaatcaatga ggcaagaatt gccgaaggca aacgtccttt tggttcaatc attcgtcagg
    11221 aggtggtcga aagaaaattt agcatcgcag atccagaaag tggttattat gtaaaaacag
    11281 aacgagaaaa acagtttgct tattctgctc ataccgttgg tgatgaaaat ggttttgtat
    11341 tagatgtcat gattacgcct ggaaatctac atgatagccg aatgctcgtg ccacaaatcg
    11401 aacgcgttaa atcacgttat ggtgtagctg ctaatgctgc gtacaaaaca ccttggaacg
    11461 tcaagtattt aatcgaacga aaactacgac ccattctccc ttatatcagt ttcgcacaac
    11521 tactcgagat ggctataaaa aatatgtatc taaatctttt atttgtgaaa gttgtccatt
    11581 attgaaaaat tgtacagaaa gccaaaaaca ccaaaaagaa atacatcgac atatttggga
    11641 agactactta gatgaagcag aacatttacg ccatacggtg tacaatcgtg aaaaatataa
    11701 aaaaagaaaa gaaacgattg aacgcatttt tgcagacgca aaggaaaaac atggaatgcg
    11761 ctacaccaag tatcgaggct tggaaaaaat caggtgcata caatgcttgt ttttgtcgca
    11821 atgaatctga aaaaattagc tacttggcag tgggagcata tgtcgttata tcttttatta
    11881 tacaaaaata gacaaaagta tcagtctaca ctcttagtaa agaggaaact gatgcttttg
    11941 tctacagtct tagagagtgg gacaatagtt ttttcttctt tacgtctttt cctctccatg
    12001 tattttccta attttcttaa tttaacgcca tgagtgccaa gccaatgtca atcttggctc
    12061 cttgtttcct tcataagtgg aaatccgttt ccgatagatc gaaccttctt tttcctctaa
    12121 aagttttttt acattctgct ttgaaataac gccatgtatt attcactaaa atttgtcggt
    12181 ttcgctcact ttttgctttc gtacattgac tacgaacagg tcagcaccga ccactttcac
    12241 attcgtaaat tttgaagaca cgtataaatc cacttttatc ttttcttcga ctaaagcgtt
    12301 gaccgcaaaa gcgacatctg tttcagaaat atattgttcc atatctaatg gtaaaatttt
    12361 ttggttgatg gtataatttt taaacataaa gcactcgctt cctttctgat tgggtttggt
    12421 cactttaatc ctacagggat gagtgctttt tgtgtacctt aaaatcagta aatatccatc
    12481 cgtgaaaacg ggtggctttt taacagccct agaagggctc accactcaca gccctatctc
    12541 tctttaacta tttaaatgga tctacatact cttgtttact tatactgtct ctaattcgat
    12601 catctgcttc ttgttctcaa atatatattt caattgtttt ttgatttaac ccaaccgtac
    12661 tcacgtagta tcctttggtc caaaagcttt tattcccata cttatatttc aaatttgcat
    12721 gtttttcatg gattaaggtt gcacttcgtc cttttaagta gcccataaaa ctagaaatac
    12781 taatctttgg cggtattctt actaacatat gaatgtgatc aggcattgca tgagcttcaa
    12841 tgatttcaac atctttcctc tcacacaatt ttcttaatat ctggccaata cttactctta
    12901 actttccata aatcactttt cgtcgatact tcggggtaaa gactatgtga tacttacaat
    12961 cccatctcgt atgtgctaaa cttttatcgc taaagctatt tcgtccacgc gtagaaacgc
    13021 gcgttttttt gtttcgtacg ctacgccaac gaaactgact aaagtcaata aaaaaagtgg
    13081 ggcaataatc agattttttt ctgactattg tcccactctc tttttattac tcttcatctt
    13141 ctactattcc actaattaat tagttgacag atacaatgct ttcacatcct gcacgaacaa
    13201 actcgggctc tgaaccaatc acttgcgagt tgataaattc aatgaccata ggaagattaa
    13261 tccgacataa agtttaatct gttgtccttc cattatcagt ttagaaatga tattagtagg
    13321 tgtaccgcct aaaagatcgc atagtaccac aaaatcattt aacgaatcta tgacagtaag
    13381 agcttttgtc tgtatgtttc agtgccatct tctggtaata aaggtaacgt gtagatattc
    13441 tcttgaggtc ccataatcat ttttgtactt tttttcaatt cctcacaaaa ttttccgtga
    13501 ctaatcaata tcaattttct actcattttt ggctctccct tatttcgaaa tcacacacaa
    13561 tgcagataaa gccacacaca agtaatacga taaaaatcgc tcgagttggg tcatattttt
    13621 ctttcctaac aaccaataaa cacctcgacg atcccagcga gtaagatttt agataatatc
    13681 atatcgatat agttttgcat attcaatgtc acatcgccaa tcgttggtac cataaaaatt
    13741 taatatcaat cattgttgca actaatgctc ctaccatgaa cactcttaat aaagtcgcta
    13801 cgtccgttaa tacagttaag cgagattaca ctagatgatc tgttcctgcc aaggcaagag
    13861 ccagaagcgg ttgcctgccc atttgccttt ggtgtgtcgg agctgtccga taaagttcac
    13921 cgcccgatcc gctctggctt tgtcgtagtg acattccgaa agcatgaatc ggcttggtgt
    13981 gtattccttt aatttgggat agccctttgg tctggtttct gccatcagct accacctccc
    14041 aacagagcct ccatcgcatc ttctgctccg acaccaccgc catcaccggc aatgatacgg
    14101 cttctggacg aaggcgtcag accgaactgc tcggaaaatc ggttcataat tttcagatag
    14161 gtctgggcaa taaacacctg tggtacctgc tgccaatagc cggatagggt tttcacgatg
    14221 ataccgtgct gggtaatgaa ttcctcggct tccttccagc gggcgtaggc ttggcactaa
    14281 ccggcaaagg cagccatatc cacatctgtc aagattccga tggcctccat ctgctttgcc
    14341 agacgcttcc attccttctt tgcctctggt tccagccact tcggacaggt cggtgccttc
    14401 ttttccggcg ttgactcttt tgcattgagt gggcgattgc ccggatttcc ctccagcttc
    14461 ttcatggctg tcggggttga ttttcttcct ctggttgcca cagtcgtcac ctcccttcaa
    14521 tgtatttctg ccagtagaca gagaacagag ccaagtcgaa acctgactct cttctctatc
    14581 tgctaacttg tatttcagtc cactcttctg atgaatcgca cccgcaggat tttcaagatg
    14641 ccgcaggcaa attccaagaa gtttctgacc gcatcataac catcgtagcc gtgtagccac
    14701 atccttccac ctcgccgccg tcctcgtcgc tgatataaat gattctgtat tcgctcatct
    14761 gttgttttct ccagtctttt gtttttttgt agtacatata tcactctaaa agtgattatt
    14821 atcaagtcgt ttgaaagcca taatgtacac aatcattcga ctggtttttg gttcataatt
    14881 gaccgccaaa gtgagcaaaa gaaagggacc tctgtctgtt tcagaagtcc ctttcggtat
    14941 ttggttctta gtggttcagg ctgtattctc aaacctcata ctcatcttcg aggtctgcct
    15001 ctgcaatgat ctggccgatg ctgtcgtaac gctggacctg catctggatg gcatccttgt
    15061 ctgctccctc gtccatcatc ttcagcagct tgtctgattc ctttttgtag gcccagaaaa
    15121 ggatctcctg aaggctggtc tcctgctttt tcttgcgaag ggcgaccttc ttctcttcca
    15181 ccgcctcgtt caacttggtg aactctgccc aagcaaggat accgtctcgc ttgattgcga
    15241 tctccacatc cttcagctcc gggatggtct ttgcaccctt gatctccttc aaatatttct
    15301 ttgccttttc gctcattgcc attagcgttt cctccttgtt tttgggattt tcccctttgg
    15361 ttgtgtgtat attgccatag tctctcgact atatcaagtc aatccacagg catatactac
    15421 acaatcattt ggagctgtat ttgtatacat tatgccgatt tatgagagaa gaaaaggctc
    15481 cagccgttcg actggaacct tgggggggtc ggtttcttag gcggcgtggc ggtgtagacc
    15541 ttgaggtagc tgatgttctt ccagacgtcc gtgcccttca gtctgaccat gccgtccagc
    15601 tctcggtcga gggtgaactt gcggaaaagc ttgatgcggt tgaggctgtt tgctagctcc
    15661 ttgccgttct tggcatccag aactgcgtcc tcgatctccg caatactaat ttcctgtcta
    15721 aaccaaaggt tgctcaaggc ttcgggaatg ccgtttgcgg tctgcagggg gttgaaagtt
    15781 tccaatacag caggggtcga aaccacctgt ccgagctgga attttgcgtt ccctaaaatc
    15841 atctgcataa tttcttccat gtgaatcatc catttctgcc gttgtggtat tccctttggc
    15901 agtacagtac tatatatcac tctgaatcgt acatttatca agaagcggat ttttgtggaa
    15961 tcaccaattc gatataatag taattgcatg aaaaatctaa gcactggatt ggtaggtgat
    16021 gattatacgt gacgatcaca tttacacctg tgattcctgc cactacagct tccctgccga
    16081 cgaacagcca gagcgttgtc ctgactgcga aaagacagca actcggctgg ataccgaaat
    16141 cgaaactgaa gattactatc gtgttcgtgc tgaaatcaaa gctgagatta aagctttgaa
    16201 tgcgggttaa cgacaggtac tgaccggcgg ctcaacaccg ccggcttttt attaccacat
    16261 cttgcggaat tcgctgggac gaacaccttc caccttgaaa aaagttctgg aaaaatagtt
    16321 tgcatctgtt atgccaattt ttgttgcaat ctcttccata cttagatttg taaagcgtag
    16381 caagtgcttg gcctgtgtga ttcgtttctg attcagataa ccgacaacag ttgtaccgta
    16441 agtttcacga aaaatcttag ctaagtactg tttattgata aaaaactctg cactcaactg
    16501 atccagcgta atcttctccg tgtaatggtc atccagatat tgttttatgt tctgcaattc
    16561 catttttttt gtagagaaat tcggcacttt gtcaggattc caagaatctt ccatcaaatg
    16621 catcagcagt tcactgagta gaacattgat tttcatatcc cggacatact cctgtccctc
    16681 tgccaccata tgaaggctcc tcagcaagtc agcatactta tctgtttttg atgttttaaa
    16741 gaccggttga ccacctcgtt caaggtattt tttgtagact tccgtcatac caatgccatt
    16801 aaaatggcac caccgcaaag tccacaaatc atcttctgat gactcatgga catacggctt
    16861 caaacagtca ataaacacca tatctcctac ttgaagcgta tatttctgcc ccttatactc
    16921 caatgtgcct gaaccaactt ctaccaggaa acaaaggtaa gatgacaatc ctgtacgcct
    16981 agatgtatgt gccttcagag cagtcagctg tccaatttcc tgcaaataca aaagagagga
    17041 tcttgcaaag aacgatgccg tgtacagcac acgatttgac ttcaccgatt tcaaagcgtt
    17101 agaaaacagt tcctccatta caagatttct cctttctttt cttactcgaa catttccgcc
    17161 agttcagcag ccataatagc ttccatagct tcggcggctt ccttgttctc tgcgctgacg
    17221 ctgatatagg tcttcagctt cggctcagta ccagaaggac gaaccacaac agagcagtta
    17281 tcttccagca taaacttcaa aacatcagac ttcggcagac cattcaggcc cttactgtag
    17341 tctaaagttt caataacctt cttaccacca attatatcga gacccttgtg gaattcaacc
    17401 ataatttcct gcatcttggt aaaacctgcc gcaccgtcaa actcgaagga atgcagggtg
    17461 ttcaaacaat agccatactt tgtataaatc tcgttcagct tatccagcag gctgataccc
    17521 tgagtcttgt agtaagagaa catttcacag attagataag cagcgttaac accgtccttg
    17581 tcacggacat agctaccgga cagatagccg taagattcct caaagccgaa gatatagctg
    17641 tcaaccttac cagccttttc caacatacca atctgctcac cgataaactt aaagccggtc
    17701 agcacatcga tggtacgaac accatagttg gatgcgatac gttcacccaa atccatggtg
    17761 acgatggtct tgaccatcac agggtcagca ggcatttttc catgcttaat tctctgagaa
    17821 cagatgtagt ccagaagtag cagaccagtc tcattaccag acaacagaac atagtcacca
    17881 tgtgcgctct taactgcgat accaacacgg tcgcagtcag ggtcagtggc caacagcaga
    17941 tccgcattgt acttcttggc atactccata cccagagaca tagcttcctt aatttcaggg
    18001 ttcggataag gacaagttgg gaaattaccg tccggcagac gctgttcttc cacaacggtc
    18061 acattgctgt atcccatctc tgccagggtg cgggtaacag gcaccagacc ggtaccattc
    18121 agaggagaat acacgatagc aacattcttg tccacttctt cgccaaacag gacggactgc
    18181 cccttcactt cttcgatgaa agcagtcaga acttccgact gaatggatgc aatcttagaa
    18241 gcatcgccat ccatctttac atcagcaaag atgtccagct tttcgatttc tgccagaatt
    18301 gcagtagcag cctcggtagt aatctgacag ccgtctgcac cgtatacctt gtagccgtta
    18361 tatttagaag ggttatgaga tgccgtaatc ataacaccgg catctgcctt cagataacga
    18421 gtcgcaaaag atactgtagg aacaggaagc agttctggcc acaagtgcac ctgaatacca
    18481 tttgcagcaa agatgtctgc ggcagtcttt gcaaacacat cagatttgat acgggaatca
    18541 tagccaacca ccacggaagc ggcttcatag ttcttattca gatagtctgc cagaccttgg
    18601 cttgccttac ctacggtgtg tacgttcatg cgattagtgc ctgcaccgat ggtaccacgc
    18661 agaccaccag taccgaaaga caaattttta tagaaagagt cctcaatgtc cttctcagaa
    18721 taggaacgca gttcttctat aatatctgca ttggcatgat tcagccaacg ctcatattct
    18781 actttataat tcatattatt atcctctcac atccagaaac tgaatcgacc cgtgaatgct
    18841 gatttcagta gaatcggccg gcacaaaaag gcagtccccc ttatagatgt ccaacgattc
    18901 attatcaaat cgaacggtac cacagccatt gacacacagc aacacacgga agctgattga
    18961 atccgcacga tagtgtaccg tctgtcttct ctcagtgttg acaatcatac gagagacctc
    19021 gaaatgcttg catctgcaaa gcaattccgc agctacacca gcacgatatt ttagaacacg
    19081 aagcggctgt ttcggttctg acaacccttt caagttcgct acttccagcg ctttgtctac
    19141 atgaagttct ctctttttac catttttgtc aacacggtca taatcgtaga gacgataagt
    19201 cagattggaa ttttcctgaa tttctgcaat tagtgcaccc gcaccaatcg catgaatcgt
    19261 tcccgcctca ataaagaaca catcattctt tttgaccgga accttctgaa gataacgctc
    19321 aacagttccc tgagcaatcg cttcacggat cacagcttct tctgttattt tctgcagccc
    19381 atagattaag ctggcatcca gtacatacca catctctgtc tttccaagct gcccgttttc
    19441 tttttcaatt gcatattcat ctgtgggatg tacctgaaca gacaaatcct ttttcgcatc
    19501 aatgaatttg atcaggatcg gcaattctcc tttggtctgc ggatgcgttc ccagaaactc
    19561 tgggtgttct tgcaacacct ccgtcaaggt catcccctga tactctgacc agacacaaca
    19621 aagctcggtc catccgggtg cgtagagcat tccaagtttc cgcaagagga gacatctcaa
    19681 ttccttttgc aaattcatca ttcaggcggt tcccccccac agataatcct ttgcggaagg
    19741 cttcaataaa aacggtttct gtataacgag ccctcctccg ttttttaata ctctaactca
    19801 aatttctgtt tatctttaac attgatatcc ttgccaagct gtacttcaat cagcttcagc
    19861 tcagtatctg ccataatggt atgccgacag ccagcactca tggtgatcac atcgccagcc
    19921 ttaacaacct gctcatatcc atccacgatg gtcttaccag taccggccag aaccacccac
    19981 acctcgtcac ggttcttgtg gctgtgataa ttcatggagt gactagcatt cagagtaacc
    20041 ttaatggtca tgctctctgc ttctacatcc ataaccttat aactacccca agacttctct
    20101 gcgaacatga tctgctgctc gaacttgtca acgaaaggct taatatagct ggactgttcc
    20161 ttatcggaaa ccagaatacc ttcccgagat gcacagatga ccacatcatg cagacccata
    20221 gccaacacag gaacatccag ttcgttcagg atgtgtacgc catcgcaatt ctcattcata
    20281 acacctttac cgataatgga ttcctccata ccttcggtca gagtattcca agtccccaga
    20341 tccttccaca taccagcaaa acgctgtacc tgaatcttgt cctcatgctc aacaacggca
    20401 tagtcgaagg aaatcttagt cagagtatcg tatttgttga acagatcctg ataatctaca
    20461 aagtcaatca gctgatgtgc acgctccatg acatacttca gcttgtagac aaacacacca
    20521 ccgttccaca gtgcaccctg tgcgatatat tcctttgcca cagcctcaga aggtttttcc
    20581 ttgaaggtat ttacgaaagc agtctgctct gcgctctcag gaatgatgta gccgtacttc
    20641 tcggaaggat aagtcggttc aatacccatg agcaccagat tagcttcgcc tttgtcagcc
    20701 tgatcggaca gagatttcag ggcctcaaaa tagtcatctt ctacgtaagg gtcaaccggg
    20761 caaacaacta cggactcttc tggatcaatg cccatcacat ctaccagata agcggtagcc
    20821 aatgcgattg ccgggaaggt atcgcggcga caaggctcta cagatacacc cacatcctca
    20881 ccaagttggt tatggatagc agaaacctgt gtcttagagg ttgcaatcgt aacagttgcg
    20941 tcgttatcta ccttcttaat ctggcggtag acacgctgaa ccatggattc atatccatct
    21001 tcagttttga aaatcttaat gaactgcttg gaacggatgt cattagacag cggccaaagg
    21061 cgcttaccag aaccgccaga cagtaaaaca atattcataa tgatctctcc tttttataag
    21121 aaagaggctg ctgcaatcct caaaaatcac agcaacctcg gtaaattagc gttcagcacg
    21181 catcgggtta ttcaggaagt cctcgtagga cagacggatg ccatcttcca actcagtctt
    21241 atatgtccat cccagcttgg ttgccttgga aacatccagc agcttacgag gtgtaccgtt
    21301 cggcttgctg gtatcccaca gaatctcgcc ttcgtaacca ataacctttg ctaccatctc
    21361 ggtcagttcc ttgatagaca gttccttgcc agtacctgca ttaacagtct cgtcaccgct
    21421 atagttattc atcaggaaca cacacaggtt tgccagatcg tcaacatata ggaattcgcg
    21481 cagaggagaa ccatcacccc agcaggtaac agtaggcaga cctgcttcct tagcctcatg
    21541 gaagcgacga atcaaagcgg ggagcacatg actgtgagta ggatgatagt tgtcgttagg
    21601 tccatacagg ttagtaggca taacagagat atagtcagta ccgtactgct tgtttagaaa
    21661 ctcacagtac ttcaggccag aaatctttgc cagtgcataa gcctcgttgg tcttttccag
    21721 ctcacttgtc agcagacagc tttccggcat cggctgcggt gccatacgcg gatagataca
    21781 ggaagaaccg aggaactgta acttcttaca gccattctcc catgcggagt tgatgacgtt
    21841 catttccaga atcatgtttt catacatgaa gtctgccaga gcgttctggt ttgcgataat
    21901 accgccaacc tttgcagcag cgagaaatac ataatccggc ttttcctgtg cgaaaaatgc
    21961 ttcaacagcg tcctgacggc acaggtcaag ctccttatgg gtgcgggtga taatattgtt
    22021 gtagtcctga cggttcagct cgcgcacgat tgcggaacca gccatgccac gatgaccggc
    22081 tacgtagatt tttgcgtctt tttccatcat aataatgctt gctcctttct aaatttagag
    22141 catctctcat tgcatgagag aaatcaattt gtctgcgatt tcgctcgcag catcataaaa
    22201 tgtatcaaaa tttgaggtgt ctagctgttt gaggtcctca atcacccgct ctacgcggtg
    22261 gaggttttcc ggtgtgacgg aaattgccag acccagtttt tgcaatttac aaatagttgt
    22321 tctatcttcc aaaactgtat cgcgggcgaa cagtgccatc ggacgctgtg cgagaataca
    22381 ctccgcaaca gttccccatc cagcctttgt aataacgtaa tctgccgccg ctatatagtc
    22441 ctgtgtattg ggggtgctgt tagaaataac ggttacgttt tctcccacaa gcggaacacc
    22501 tagcgttgta atgaaatcat acggcgcgtg actgacatca atcggctgtt tgaaccgcgc
    22561 ggacataccg agtgcgacaa atacaggcgg tttttcatgc tgtgatttaa tgcgttgcac
    22621 ttcgtcgtta tgaatcggac gcgccacaac ggaagtcggt gttttttcgc tgttttggag
    22681 aaattccaac atttcttcgt tatttagcgc ataaagcatg gtgtgctgaa ttttaccgta
    22741 acgcttggca tattctttcc aaatatgttc gggtaaaaat tcgcgataca gctctgtcca
    22801 agtgaagttt cctacataca ggaaaggtac tcctgccata tcgcaggcat ccattgccca
    22861 gattggcata tcgcaaagca ctgcgctgac agcttctcgc ttgagccatt cggcttctgt
    22921 ctttgccaaa gaaggaacct ttgccaaaaa atattcacag atctcggtca gcttacccgt
    22981 gtccaccagt agggtgccct cacggttaat cagaccgata tctgtgttta ttggacgata
    23041 gcgcacgcgg tctatcagct ctttgggcag ttcctgctcc aaattgcgct ttgcaaaatc
    23101 cagatgattt gcgccgcaaa ccagtgtaat cttcgcttcg catcggcaga gtgttgccat
    23161 aataatgggc agattccgcg cgatatgtcc gaagccgtga ctggaaagat agaaacagat
    23221 atgtttattc aatgtcagct tacttgactg cgttcttcat tgcagcttcg cgctttgcca
    23281 attcacggtc atgctttgcc atgatgttaa ccagttcctc gtaagaggtc ttctgcggat
    23341 tccaaccgag aacggtcttt gccttagtcg ggtcgcccca caggttgtca acgtcagtcg
    23401 gacggaacca ctgtggattt acacaaacga gcatcttgcc ggtttcaaca tcgtagccct
    23461 tttcatcaat gccagtgcct tcccaacgaa tggtcatgcc atttgctgcg aatgccttct
    23521 cggtgaagtc acgaacagtg tgctgctcgc cggtagcgat cacgaagtcc tcgggagtct
    23581 catgctggag aatcatccac atgcactcaa catagtcctt tgcatagccc cagtcacgca
    23641 gggagtccat gttgcccagt tccagatggt cctgcaggcc ttctgcaatg cggccggcag
    23701 cgagggtgat cttacgggtg acgaaattct caccgcgacg ttcggattca tggttgaaca
    23761 gaataccgtt gacagcgaac atgccataag cttcacgata ttccttagtg atccagaagc
    23821 catactgctt tgcaacagca taagggctgt aaggatgaaa cggagtggtt tcacgctgag
    23881 gaacttcttc aaccttgcca tacagctcag aagtagatgc ctgataaatc ttagtcttct
    23941 tcgtcaggcc cagaatacgg caagcttcca gaatacgcat aacgcccaaa gcatcaacat
    24001 cgccggagta ctcaggaaca tcaaaagaaa cctgaacatg gctctgtgca gcgaggttat
    24061 agatttcatc aggctgaaca atgctgatga tacgaatcag agaagatgag tcggacaggt
    24121 caccgtcatg aagggtgatc ttgcccatca aatggtcaat gcgagcggta ttggaaatag
    24181 aagcacggcg agtgataccg tgaacttcgt agcccttctc tagcaggaaa tctgccagat
    24241 aggaaccgtc ctgaccagta ataccagtaa tcagcgcttt tttcatgata cattatcctt
    24301 tcctatgtaa gtgataaggc catataaatg gctcgtaatt tctaatactt ttcaagaata
    24361 cacgataatt ttcgatattt catagaacaa tctacgcacg cattagcaca atattagttt
    24421 ttcgtg
//
LOCUS       DAKBVG010000013        24033 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000013 DAKBVG010000000
VERSION     DAKBVG010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 24033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24033
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.013"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(65..745)
                     /locus_tag="OUD09_000442"
     CDS             complement(65..745)
                     /locus_tag="OUD09_000442"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCT9691741.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            871..1740
                     /locus_tag="OUD09_000443"
     CDS             871..1740
                     /locus_tag="OUD09_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCT9691742.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            1766..2281
                     /locus_tag="OUD09_000444"
     CDS             1766..2281
                     /locus_tag="OUD09_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9691743.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(2328..2921)
                     /locus_tag="OUD09_000445"
     CDS             complement(2328..2921)
                     /locus_tag="OUD09_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCT9691744.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(2946..3125)
                     /locus_tag="OUD09_000446"
     CDS             complement(2946..3125)
                     /locus_tag="OUD09_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9691745.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(3202..3711)
                     /locus_tag="OUD09_000447"
     CDS             complement(3202..3711)
                     /locus_tag="OUD09_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9691746.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(3807..4187)
                     /locus_tag="OUD09_000448"
     CDS             complement(3807..4187)
                     /locus_tag="OUD09_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCT9691747.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            4517..5266
                     /locus_tag="OUD09_000449"
     CDS             4517..5266
                     /locus_tag="OUD09_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HCT9691748.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(5450..6727)
                     /locus_tag="OUD09_000450"
     CDS             complement(5450..6727)
                     /locus_tag="OUD09_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9691749.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(6806..7450)
                     /gene="pcp"
                     /locus_tag="OUD09_000451"
     CDS             complement(6806..7450)
                     /gene="pcp"
                     /locus_tag="OUD09_000451"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HCT9691750.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(7649..8593)
                     /locus_tag="OUD09_000452"
     CDS             complement(7649..8593)
                     /locus_tag="OUD09_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HCT9691751.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(8595..9281)
                     /locus_tag="OUD09_000453"
     CDS             complement(8595..9281)
                     /locus_tag="OUD09_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HCT9691752.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            9755..10075
                     /locus_tag="OUD09_000454"
     CDS             9755..10075
                     /locus_tag="OUD09_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HCT9691753.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            10229..11134
                     /locus_tag="OUD09_000455"
     CDS             10229..11134
                     /locus_tag="OUD09_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCT9691754.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            11131..11904
                     /locus_tag="OUD09_000456"
     CDS             11131..11904
                     /locus_tag="OUD09_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCT9691755.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(12085..13509)
                     /locus_tag="OUD09_000457"
     CDS             complement(12085..13509)
                     /locus_tag="OUD09_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCT9691756.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            13720..14166
                     /gene="nrdI"
                     /locus_tag="OUD09_000458"
     CDS             13720..14166
                     /gene="nrdI"
                     /locus_tag="OUD09_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCT9691757.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(14231..16213)
                     /locus_tag="OUD09_000459"
     CDS             complement(14231..16213)
                     /locus_tag="OUD09_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCT9691758.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSAATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(16227..17123)
                     /gene="murQ"
                     /locus_tag="OUD09_000460"
     CDS             complement(16227..17123)
                     /gene="murQ"
                     /locus_tag="OUD09_000460"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCT9691759.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(17126..18187)
                     /locus_tag="OUD09_000461"
     CDS             complement(17126..18187)
                     /locus_tag="OUD09_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HCT9691760.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSADARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(18398..18925)
                     /locus_tag="OUD09_000462"
     CDS             complement(18398..18925)
                     /locus_tag="OUD09_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCT9691761.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(18992..20494)
                     /gene="eat(A)"
                     /locus_tag="OUD09_000463"
     CDS             complement(18992..20494)
                     /gene="eat(A)"
                     /locus_tag="OUD09_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HCT9691762.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKSQPKHLSISYVRQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(20752..21873)
                     /locus_tag="OUD09_000464"
     CDS             complement(20752..21873)
                     /locus_tag="OUD09_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCT9691763.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALGGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPHGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            21998..22939
                     /locus_tag="OUD09_000465"
     CDS             21998..22939
                     /locus_tag="OUD09_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCT9691764.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFRSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEAKLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKTAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYNPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAVWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(23089..23985)
                     /locus_tag="OUD09_000466"
     CDS             complement(23089..23985)
                     /locus_tag="OUD09_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HCT9691765.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFNMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMDFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLAYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPIPSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
BASE COUNT         6989 a         5023 c         4288 g         7733 t
ORIGIN      
        1 gctggaaaaa ttaagttttt cacatacaaa gcgaactgca ctataacagg aaagcgctgt
       61 tttattacac tgtttcaggc agttgccaat caatcggttc ttctcctaac gcaaggagcg
      121 catcattcgt tttcgagaag ggtttggaac caaaaaagcc acgacttgca gataacggac
      181 tgggatgtgg agatgttatg atgatatggc gactcttatc gatcattttg attttttctt
      241 gtgccggttt tccccacaag ataaacacga ttggtttttc ccgttcattt aatttttcaa
      301 tgatcgtgtc tgttaatctt tcccaacctt tcccgcgatg agaatacgct tgcccttccc
      361 gaacggtaag aacggtgttg agtaaaagga ctccctgttt agcccatgac actagatttc
      421 catgttgaac aggtgcgatt ccaagatcat cataaagctc tttatagata tttcttaacg
      481 atggcgggat ctttacgcca ggttgcacag aaaaagataa gccatgagct tgattttctc
      541 catgataagg atcttgtcct aaaatcacca ctttaacttc ttcataagga gtcaattcca
      601 atgcttcata gatatgatac atatctggat ggatcttttg ggacgcatat tcttttttta
      661 aaaattctct tagatgcaga taataatctt ttgaaaattc tgaggacaaa acttcttgcc
      721 aactgttatg aatgatttct ttcaaaaact ccacctccta ataaaaagaa taactcacat
      781 tttcttcaat cacaagggac aatcttcacc ctcagcactt gattaatatg gtaaactagg
      841 aacaagaagc agaaaggaac gataatcatt atgatcaaat taattgcatc agatatggac
      901 ggcacattat tagatgccca tatgagtgtt tctcctgaaa atgccgaggc gatacgtttt
      961 gcaaatgatg ccggtgttga gttcatggtc gctactggtc gaaactatca agaagctcgc
     1021 gcagctttag atgaggtagg aatcgattgt gcgatgatca ctttgaacgg ggcacaggta
     1081 tttgataaag aaggaaattc attgtttacc gtgtcgattc caaataatca agcaatctct
     1141 gttttagata ttctagatgc taacggtatt tactatgaag tagcaaccaa cgatggtctt
     1201 tattctgaaa atcaagcgaa acgaattgaa agttttgctt ccatggtggc tacccattta
     1261 cctcacctta cgtataaaat ggcgattgcc atggcctcag ctaagctgga gttattgcac
     1321 atcacatacg tggacagtat tcgtgctatt ttggaagaaa agaatctgga agttttaaaa
     1381 attatttgtt ttcatacaga agggccagct gtattaggtc cagttggaaa gcagatttct
     1441 cttttaggcg atctagccgt tacttcctct ggtcaaaaca atatcgaagt gaaccataaa
     1501 aacgctcaaa aaggaattgc tgttgcccat gtcgctcacg agcgtgggat ttctttagaa
     1561 gaagtcatga cgattggcga taacttcaat gatgtcagca tgctgcaagt agcaggagtc
     1621 agtttcgcaa tgggaaatgc agaaatcgaa gtaaaagatt atgccaaata tacaacggac
     1681 acaaaccttg aatctggcgt aggaaaagcc atattgcgcg cgattgcaga agacttgtag
     1741 ttttatctag aaaagaggga tatagatgtc agaatttttc attcaggaaa agcagcttgg
     1801 acgtgcaact cggacaattg tcaaaaacga aaaaggacat tccctgtttt tgatggttgg
     1861 tcgctgggga acaaagggcg atgcattgtc tctttatgcc atgaatggcg atctcgttgc
     1921 tcacatcaag cagataagcc tgacattcgg tacgcgtttt gagctgtata aagagttcga
     1981 aaaggtggga accatgcaga aaatattcaa ttggcccggt gacttttatt atattcgtag
     2041 acttcactgg acagtccaag gtgatatcta taatcatcaa tatcaaatcc atcatttcaa
     2101 tcagcaaatc atgaaaatgg ataaagcaac acttttttca ggagattact atgtattgga
     2161 tgtctcaaat gatgaagatg ctccagtttg tatttgtatt gctgcggttt tagattactg
     2221 gctctaccgt aaagacaaaa gtcgcgataa gcctgtttct taccgactta acttaagtta
     2281 aaaagatgta cctgaaatgc ggaaatacgc atttcaggta catcttttta gatattcttt
     2341 tgcatatgat cataaagatg accactgatc atccgctgac cgacaacttt gaagtcatgg
     2401 cgttcgtata attttttagc ctttggattt ttttcatcca cacttagacc gatgacggag
     2461 cgattagctt tcttcgccaa tttaggtaat gcttctaata atcgcgaacc gatgccttgt
     2521 cctctaaaat cttctgatac actgatagag tccaaatacc attcgttggg aaacgtttct
     2581 ttatcaatga acaaacgtgt agacgcatct agctgatatt tctctaagat aggtaccagt
     2641 ggctgatcga tgattgattc ttcttcatct gtataaccgt aagcaactcc tgcaacttta
     2701 ccgtctattt cttctacgat acctcgtgaa taactgtaac gataatttgg atcagtgatt
     2761 gcttcttcta aaatctctaa agtcttttgt tcccctgtct gcttcaaaaa tggcagttcc
     2821 atatctttta gaatcactaa gatcaatggg gcgatttctg gagcatcttc ttttctcgca
     2881 aaacgaatca tagtttcctc tccttttctc cattctaaca tgcagaaaaa gaaatcgcaa
     2941 aatcgttatt ttaaaaaatc ttcaatcttt tcagtaatga aataaccatg agtaacgatt
     3001 cttgccgctt cagttcggca gtaagggcag acgactggca tttctttatc attatgttcg
     3061 actacattat tgacttcttc gtatgcaaat ttttttccgc aattagggca tttcattttt
     3121 tccatgttat ctcaacttcc tttcatcttt actctcttag cttactctta tattgtaaaa
     3181 aagagaagca atatccttct tctatgcaga tatccatacc tgacggtcct gttctccatc
     3241 tattaatcgg actttataat aaggacgttc atctttgact agaagtgtcc actctttcac
     3301 ttttgctttt ttcccgtagt agctgactgc ttccttcatc gctgcttgcg gttgtttgaa
     3361 ctcttccgtt ttttctatat caaaaagatg ttctgcggcc ttttccgtgt catttcgtac
     3421 ggttgttttc ccagtggatg gattgactgc tatgacttgt ccttcacaaa aaaactggta
     3481 ttcatatgtt gcattcgtag gattctccaa atctttttca actgcaaaat gaatagcaga
     3541 tatgttatgt tcttttgttt cgttttttac gattgctgct gcttttttca atgagatccg
     3601 atattcctgg tcactaatat catccatgtg aaaagcggca ttgagcagtc ccgtcatacc
     3661 ggtaatccct attacgatga gggcagcaac accaaacatc atttttttca ttcttcattc
     3721 tcctgtttct tttttttaaa gaatatcaag aaatgaacga atgaaaaatc gaatgaaacg
     3781 aaaaaaaagt gacaattttg ttatctttat tcgtgcagtt ctaatggcaa tccgtctgga
     3841 tcaaagaaaa acgtcatttt ttttcctgta aaggtatctt ctctaatcgg ttctgattcg
     3901 atccctaagg actgcaattc tgcaattgtt tcttcaatat cgtctacttt gaaagcaaga
     3961 tgacgcaagc cacaagcttc tggatagttt ggtcgttgtg gcgcattagc aattccaaaa
     4021 atctccaatt cagcacttcc tagtttcaga tctaatttat agtcatctcg ttctttgcgg
     4081 taattttctc tgattacttc aaatcctaat ttattaacat aaaaatcacg tgatttctga
     4141 taatctgaaa caatgatggc tatatgatgg atcgttgtaa ggttcatatg agcactccta
     4201 ttcttcgttt aagctcggat ctatattaat ctccagttcc attgtaaatg tgtcagattg
     4261 aaaaaaatag ctgtttttcc tttttataag aaaaaaggca aaaaaaacag aaagtaggtc
     4321 ctccaagaca ctcttcctgt tcccaatata ataaatgaaa gtaagaaatt atattttcaa
     4381 gaaaacaaac agaaaattat cataacacaa attatttata attgaaagac tagttttttt
     4441 tcaagaaaag catacaatag attgtgttag taaatacaga acacactaca tttagtgtct
     4501 taaggagtcc tcatacatga agtcatattt tcagttttta gctcatcagc taaaaggctg
     4561 gccgcaacaa aattattatc ttttcttttt cagtcttggc tgccaggtca tgactcttgt
     4621 caatagtccc atcacttggc tgtccgttat tacatttatc ggtaccacgc ttggtgtatt
     4681 gtgtatttta tccatcaatg cggcaaaatc agtcaacggt attttaggta ttttgtctgc
     4741 gatttgtttt attatcgttg ggttttcagc aaagaactat ttaagtatcg gtgaacagat
     4801 tgcttatatg atcacattgg atatccccgt attgttaagt aaagaatgga atcataatat
     4861 ggcttcaaaa atccgaaaat tcactggaaa aagctggttt atcgcaatca tcagtacttt
     4921 ccttgtctat cttgtttcag gattcctgat tggtcgttat acagatgatc ctcggccgtg
     4981 ggtagatgcc attgcttttt ctattagctt gactgctggt atcatcagtt tcttgcgtta
     5041 caacaaccaa tattactggt ggttagcttc agggcttgct caaatggtcc tttggtatat
     5101 ttcatttacg catggatctg caagtttggc gatgttcgtg aacagctctg tttacttgct
     5161 gaatgatgtc cttgcattta ccgtttcacc ttggtataac caaaaagaac gtgaacgtat
     5221 gcaaaaaatg gaagaagaat actacaacgc acaacaacac gcataaaaaa actgagcgtg
     5281 ggacaaaagt aaagaacact tttgtctcac gctctaaaca cgtataaacg gcggaatcag
     5341 aagcaactcc ttcggaaata agctgaaatt cacaaaaatt tgaaaagcaa ttttcgtgaa
     5401 tttcttctta tttcttggag ttaaacgctt ctgtcccagc ctctattttt tatagtaatt
     5461 ccttcagata ctttcttgcc tcatcattga aacgcagtga ttcttctaac ttctgataat
     5521 attccaacgc ttgtttctga gcttcagtaa aacgataatg ttttcctttc catttgtcga
     5581 ttgtctcttg cacatttcca gattggatat ctttcactac tagttgccat ttttcttttg
     5641 agactgtacg gtctttctta tcagcagatt gtatagcttc tcgttcttta cgcaatactt
     5701 ttaaaaaacc atttctgact atccgaggaa tgctgtcgaa gaacccttct gtaaaaccat
     5761 agcgacaata ttgcttccaa ataaatagat agatttttgg ttggatgaat cgttggtttt
     5821 tcgcaaaata ttccatctga gcagtgactt ctggtgttcg ttgacgtttt gtctttctca
     5881 acacggaaat aaatgtttca aaataagcat taggatgtac tggcacttct tcatatacga
     5941 tggattgcca atctttacgt aaaaatgcgc gatagttgat caagaattct tctacatcct
     6001 ttactaattc ttctgcaaag tcgtcaaata gttctttttt tatctgtcgc ttttttgact
     6061 gcccattgat gtgttcactt attttcttga tttcattttc caatgcttga taatccgcta
     6121 tgtaaatcgg catttctact tggcgaaatt ctgcgagccg ctgagctaat tttatatccg
     6181 gtaaatatgg ctgtttcatc cgacgatttt ggtacaagac aaaacagtat ttttgccata
     6241 gtccttcagg aaaatcgata ttcttctgct taagccataa taattcgtct aaggccagat
     6301 caacttttgt cattccctga tggatcgaag ctgccactgt cggagagaca tgcaggtgat
     6361 cggcaaagtg gttgatgcct agttttagga tctttccggt ttttggcgat ttaacgatgt
     6421 attgcacttt taattctcta ccacactcac aatacaattt ttgctggctt cgttttgttc
     6481 gaaaaaacgg attgatcttg aagtcagaaa acacccaatc tgttgcagct aaataatgtt
     6541 cttccataaa acgagagctg attagatatc ggcggtgctt atcgactagt tcccgctgtt
     6601 ttttcgttaa ttttgcatag atctcccaac gttgactgct tgtgtattgt ttagaaattt
     6661 tgatgggata gcttttgctg atttcttttg gaaaccattc ttcaaagacg gattgccatt
     6721 cttccacggc tgtttctttc ctttctaatt acaaaaagct ggctactaac ataacgttag
     6781 caaccagcca gtgtttcttg ctaaattagt gagttgttcc ggcatttccg gaaatatctt
     6841 ctttattttc tactgctgct tcaactgcat aagtcaaaga agaagcaatg tcttctaaag
     6901 acatagaagg cgtacccact ggacgatcga tcacttgatc gggtgagaat ggcacgtgga
     6961 tgaacccacc tcgaattgcc gggaattgtg tttcgatcaa atgaagcaaa cgatacatga
     7021 tatcattgca gacaaatgtt cctgctgtat aagagatgta cgctggtaga ccatgatctt
     7081 gtacattttt tgtcattgct ttgattggca atgaggtaaa gtaagcggct ggaccgtttt
     7141 cttctaaagc cgtatcgaat ggctgatttc cttcattatc agggattctt gcttctgcta
     7201 gattgattgc gacacgttcg aacgagactg ctgaccgccc acctgcttgt ccgacacaga
     7261 tcacgacatc tggttggtgc tgttggatgg cttctttgac tacttgactg cttttttcga
     7321 atacggtcgg tacttccact ttgatgattt cagcaccgct gatttcatct ggcatttttt
     7381 ttactgcttc ataggctgga ttcactttgt ccccaccaaa tggatcaaaa cctgttacta
     7441 gtactttcat ctgttgttcc tccgtccgat tgatttaaca tatggttatc ttttatcata
     7501 ccattttcct tgaacagcgt aaataaagga aacaatagtt taaataacat aacagatcaa
     7561 acttaatata ttcttataaa aaaatagtgt tccactacaa tgttagaaga ggttgcgaca
     7621 attattctgt cacaacccct ctcttctatt atttaaatag aatcatataa gcaatttgga
     7681 aaactagcat aaataacgca ataaacaact ggtttttgat cactccgtat ttgtctttca
     7741 tctccagcat cgctacaggc acgatattga agttagcagc catcggtgtc agcagggtcc
     7801 cacaaaaacc gcaagtcaat gcaaccattc ctattaacgc tggattcgct ccatgactaa
     7861 atacgaatgg tgcaccgatc ccaaccgtca tcacggtgat ggccgcaaat gcattcccca
     7921 tgattaccgt aaatatcacc atgccaagag cgtaaatgat gatgccgatg ttcacatttc
     7981 cttctggaac gatcgtacct actgcattgg aaatcactgt cccaactcca gcgcttgtaa
     8041 ataccgcacc tagagaagct aaaagcattg gcaacatgct taacggccct actgtgccca
     8101 gcatatctgc tgcatcatct aaaaatgtac ttggtcgatt atctcgagaa aagaacatca
     8161 agattagcat ggctgcaaaa acaccgacgc ccacgccgac taaagcacct aaattggtaa
     8221 atagagcaaa caagatcgca aagacaccga tactcaaagc tggaataaaa attttcattc
     8281 ctagtttgtc tgacattttt tccatatatt cttttgatgg aagccggctt tctcctttgc
     8341 tgactttctt agcaattgct ggtatggtca tcgcaaagat caagccaccg ttcacgattg
     8401 ccggaagcca tcttcctcct gcaataacta cccctaagac cgtccagaaa aaggctgacc
     8461 cataacgatg aggattggtt ttatctgtta aatttcttac tccagtgtaa atcaatacga
     8521 gtcccatcac gatataaagg atctccagta atttatcgcc taataaaata tcaggatttg
     8581 taaaaaagtc catcttattg cccctttcct ggataatatt tcttcgctaa tttcttttct
     8641 ttaagataga agtagacact tgccacaact aaagccgtga tagccactgg aacttccact
     8701 aaagcaagct gtcccaatgt tacgtcatat cctagatctt tcagtgttga ttggactagc
     8761 aacgcccctg ctcctccgat gaatagtact tggccaaaga accagcagac attttccata
     8821 gctgaagcca taccttttag ttcttcttca tgcttaggat ggacttctaa cccttttgat
     8881 tcgatggtcc ccaatgccat cggtaaaatg attggacgaa caaatccagc aactccacca
     8941 aaactcacgt taaaagccgc aaagatcatt cgaaagatcc cataaatatc tacaatcata
     9001 ccagcagata ctttcttgaa tcgcttgatc aaggaagccg ctgattcttt cagaccattt
     9061 ctttcaagcg ttcctgttgc caacataatg atgataaaaa tcgccatccc gcgattgttt
     9121 acaaaacttg tacctaaaag ttctaacatt ccttgaatgc caagaccgcc agtcaaagct
     9181 gtaacaacta aagcaaccat tacgatcaat atcgagtcta atttaaatgc aaacccgaga
     9241 ataacaatca agacgcctaa aaggctaaga taattttcca tatattttcc tccataaaat
     9301 gacaatattt ttttaattat aaagcaaaac acataaaaaa tatagttgtt ttctcacgaa
     9361 atagacacta tttttaaata tttttcgtta tttttttatc acaaaaatta tcttacttat
     9421 tgtaaggtaa agcttttttt aatttattgc ccgataatat atagaaataa ggtctagtta
     9481 ctgttttttt taaatgatta ataaataaca acttctttct atgaaattgc aaagcattca
     9541 tcttcttttc cgctaacgat atatccgtca gtgcttggta cactttacta aaaagtatga
     9601 gattgccgat atctggatag gtgtgctata ctacagaaaa agatatacga tctgccaccg
     9661 ggtaggataa agaggatgat cattccatta ggtcttttaa gggatgattt tcctcttttt
     9721 gtgtattttt ttatagttaa ggaggaatgt aagtatggca tggatggaat taatcatagc
     9781 aggaattttg gaagtattct ggtcaacgat gatgaaatgg agtgatggtt tttctaaaat
     9841 caattattct ttttacacag tgattggcat gattgctagt ttttactttt tgtcaaaggc
     9901 aatcaaaagt ttgccgatga gtctctctta tcctatctgg acagggattg gcgcagttgg
     9961 ttcggtcata attggtgtgg tcttgtttca tgataagctg aatctttcca cttggttctt
    10021 tgtggggatg ctactcatca gtatcattgg catcaagatt actagcggcc actagacact
    10081 tggaataagt actgatcaga aaaaataagc ctttttattt aatattcaat ttttttatga
    10141 aaaaataaga aataagtcca atgacctagc gacttctcca ctcatcaggt gtatgcttgt
    10201 actatctata gaaaaggagt gaatattttt gaaaagtctt tcggtccaag aattatccaa
    10261 gaactttggt cacaaacaag ccctcagcga tgtcagtttt agcttatccc ctggcaaaat
    10321 cgttggatta gtaggtccta atggcgctgg aaaaacaacg atcatgaaag ctattttagg
    10381 actgatccat tactcagatg gaacaatctg tattggtgaa aatgagattt ctccctctag
    10441 tcataaaggg ttggaacaag taggagcatt gatcgaatac ccgggtatct atccattctt
    10501 aacaggctat gatcatctga agttattctc tgaaaccaac gatgtatctg ctatcgacac
    10561 tatcgtgaat cagctcaaga tggaaaagta cattcacaaa aaagcgaaat cctactcgtt
    10621 gggaatgaaa caaaaattgg gaattgctct tgccttactg aaccatccgt ctatcgttat
    10681 tttagatgag ccgatgaacg gtttggatcc acaagcaaca agagatgtgc gaaaaatcat
    10741 taccgacttg gctactcagg gcacctcttt cttgatctcc agtcacttac tcagcgagct
    10801 tgaaaaaatg gtcgatgagc tgatcctgat caatcaagga aagatcatca aacaatgtac
    10861 aatggctgaa ttgaaccaaa cagcgcaaga tttcatcgtt atctctacaa caaatgatct
    10921 agctgctcga acgatcttga cagaagctgg atatactttg gaagatcctg ataaaatcaa
    10981 gcttttaaaa gaaaacgaag aattattagg aaccgtgatc cagctattgt ctgcacaaaa
    11041 tatccatgtg acagacgtcc agcatctgac gaatgatttg gaatcttctc tattggaaat
    11101 tctggattca gtaaaggggg aagaagaaaa atgacattgc ttgtaaaaca agaattattt
    11161 aaattgatca agaaaaagtc aactgctgtt ttatctgttc tattagttgt tttgctcata
    11221 ggaacagcac ttctagccaa aaaatacacg acgatcattg atccagttga aatgactgct
    11281 cagcttttta gtgccacatc ttggattgtc tttatcatga ttgcagctgc aagcacgatc
    11341 atttcgatgg aagcacaata tggtacattg aaaaatctcc tttatcgaaa gtattctcga
    11401 ggagaaatat tagtaagcaa atggatcaca ttagttattt attctgttta tttatacctt
    11461 ttagctatta ttgtcactgt gttgatgaaa ctgattcttt tcccttcgat ttccttcacg
    11521 gagaaggttt ctactggtca aacactgatc caaagcttgt tcacttatac tttgggtagt
    11581 tatatcggtt tatggctgat tttaagttta gtgttgatga ttgcttgctt tatcaatagt
    11641 tccggtgcat ccatctcagc aggaatcgtc ttttattttg cttcgtcaat catatctggt
    11701 atccttattg cactcatcca aaaatgggag tggatcaaat ggaatccgat cagtatgttg
    11761 aatctgcaaa accaaattgg aaacgaagag atcatgaagc aattgactca tctttctact
    11821 aatcaaatgc tattcggtaa tttagcttac atcgttttgt tcttagcatt aggatatctc
    11881 gtatttaagc gaaaaaatgt atgatgactc atcaaaaaag aggctgtgaa agagacgttc
    11941 agccttattg ccgattggct gtctcttaaa caccgtttat tctgaaaaag gagctgtgac
    12001 aacatttgtg tcacagctcc tttcctagta tgtttttgaa taaacgacag gctcacaacc
    12061 tctctttata cttcttttat acttctatac gtgaagcatc ccagtggcaa ataaaaccat
    12121 tgcaatcact ccgaccatcg tcacacctat aagtaaaatc ttatctaagt ggatatgttg
    12181 tacaggctcg ttttgctcct tacgtgcttt gaggtaaaaa taaatgcctg gcaagaaaag
    12241 aacaagaaca agcattactt cattaaaggc tacgactagc atagcccata cttgaaaggc
    12301 acttgctaaa agccctacta aaaattggac ccattctttc cgctgataag aaagtttcat
    12361 ttggtacaat cctaagaaaa gccacgtgaa gaaaatagct gctgtaccaa gtgtgtaagc
    12421 aaagttataa gcactatcag tgaataataa tgaaagtaaa aagacattcg tacataacgt
    12481 cgtcaatacg atagcataag ttggtgcccc atttttgttg agttttcccc atgctttagg
    12541 caaaagtccg tcttcagcca tgtttcccat tgtttcaatc ggtaagatcg tccatgagag
    12601 ccaaacacca actaaagaaa tgatcaaacc gccgttgatc acgactgctc cccatttacc
    12661 aaccatcatt tcaaacacat agcccatagc aggctgcgcc ttgatcgttt gcagctgatt
    12721 catcgtcatt gtcccatatg gaagcatgga aaccaagacg taaataataa tcaagccgaa
    12781 tatccctaga atcgttgctt tcccaacatc tgattgtttt tttgctcgtg aacctacgac
    12841 agaggctcct tcaataccaa caaataccca aagcatcacc atgatgcaac tcaatacttg
    12901 actggaaaac gggatcggct gtccattcgc attcaatata atgtcgctag aaaagttatc
    12961 aatgaattgt tggatcgaaa acgtgctgat agctactaca ataaataaaa ataatggcac
    13021 taatttacag aatgtgacga tagcattgac gaaactagca ttttcgactc cccgattcac
    13081 tagccatgca tatccccata aaaagactgt actcatcacg atcgatgcca gattttgccc
    13141 accttcaaat actgggaaga aatatcctaa tgcgctcatc aacaatgttg caaaagctac
    13201 gttgcctaac cagcatgtga gccagtaacc ccaaccgctg ataaaaccag cgaaacggcc
    13261 aaaaccttct tctgcgtaac taaagatccc gccttttaaa tctggacgtt ttttattgat
    13321 attattgatc gacaaggaga gcatcaacat tgctgttcct acgagtaccc aactgaaaat
    13381 agctgggccg ccagccgatc cggagatgtc cgtcataata ccaaatacgc cacctccaat
    13441 cgctgaacca attactaagg cagcaagtgg aattaaacca attccttttt tctgttgctc
    13501 tgtatccatg taagcatcct ccaaaataaa accgctttta tcaaaaagta taacaaacct
    13561 tttttatttt tacttaatta ataaaaactt taatagaaat ataattctac cggctttttt
    13621 ctcattatac aagcaaagcg cctagtccct ttccaaatta caccaccaaa aaacttaaaa
    13681 aagatgctat actaaaagga aagaattgga ggatgatcca tgaacatttt atatatatcg
    13741 atttcaggaa atacgcgtgc ctttgccaaa catctagcag aatatgctga aaaaatgcat
    13801 gcagaagatc ctgtcaatcc tgaagttact ttaaaagaaa tccatgaaaa tagtgatttt
    13861 acgaaagaaa cagaaccatt ttttactttc gttcctactt atttagacgg cggaaatggt
    13921 ttagataacg gtgatacaga aatcttgacc gaaacgatgc gtgaatatct ggaacatgaa
    13981 gacaatcata aactttgtct tggtgtagtc ggtagcggaa ataaaaactt caataatcag
    14041 tattgtctaa cagccaaaca atatgcacaa acatttggct ttcctttctt ggcagattat
    14101 gaattacggg gaacaccatc tgatgtgaca cgaatttatc agattttagc agaaaacaat
    14161 aaataataaa aaagaagaag ctgaaaaata gatttcagtt tcttctttcg tttctattgt
    14221 ttctatcata ttaagctttg acatccatca caggtgcttt tgcttttgct ttacctgttt
    14281 tttccaagac aaagtttttt acacgatcca tattggttac caagacgatc atggatgttt
    14341 ctttgcctgc tgctttgatc gcttctaagt cagcttctgc tacaagcgta tcaggtgtca
    14401 cttgctgccc ttctgttact ttcacatcaa atggcgtacc gcctaagtca actgtgttga
    14461 ttcccatatg aagcaagatt tctaaaccat ttggcgttgt gatcccaata gcgtgtttcg
    14521 ttgggaagac agagctgact accccagtaa ctggagaata gatatgtccg ttttctggtt
    14581 ctacaaagta gccttcaccc atcatctttt gtgcaaaaac tggatcactt gctttatcta
    14641 aagaaacaac tgttccgtca gcaactgatg agaggatagt cacactttca gctgaagttt
    14701 gaggtactac tttttcaggc acttcattta ctgtggctgt ttcagtcgct aattcttctt
    14761 tttctaaacg gtctttagga ataccgaaga agaatgtagc aacgaagcca ccagcataag
    14821 ctgccaataa tccaaggatg tagcctaacc atttaccgtc agagatcaat gggatcaaag
    14881 cggctcctga aggtccaata gcgatagccc cgacatttcc gatcattccg atcactgccc
    14941 cgccgatacc tccaccgata caagcagtaa taaatgggcg tcctagaggt aatgttactc
    15001 cgtaaatcaa tggttcgccg attcctagga tcccgactgg taaagctcct ttgatcattt
    15061 ctgttaattc tttatctttt ttgcagcgaa tccataatgc caatgctgca cccacttgtc
    15121 ctgcaccagc catcgcaaga attggaagca atagcgtacg accagtttca tcaatcattt
    15181 gcacatggat tggagtcaaa atttgatgca gtccgaacat aaccatcggc aagaataatg
    15241 tgcctagaac gaagccggca aatgctccac caacgccaag tacccagttg atcccaccga
    15301 ccatgccatc agaaatgaaa cctgctaaag gcatgatgaa gaaaatttca atcagaccga
    15361 tggcaatcaa tgaaagagtt ggtgtaacga tgatatctac cgcatcaggt actactttat
    15421 gcagtttttt ctcaacgatt gacaatagcc aaacagcgaa gatgacacct aggatcccac
    15481 cttgtccagc tgcaagagct tcaccagtaa ataagttttt cagtggtgct tccggattca
    15541 tccctgtcag catcacaacg gccccgataa ctccaccaag agttggtgtt gcaccaaaaa
    15601 cttgtgcagc attgatacct gtatagataa ctaggtagct aaacatcccg tttttcaaga
    15661 tgttcatcac atcgatatat tgttgccatg ttgctgcatc aagtgttccg gctgtgacta
    15721 agtttgctaa aactgcggca atccctgcca cgatcccagt accgacaaat gcaggaatca
    15781 ttggcacaaa gatattagaa atgtctttta ataccgattt gattttagat ggtttttgtt
    15841 tcgatttttg tgccgctttc atttcttgtg cccgttcgtt tacacggtct tttccagatg
    15901 ctgatgaatt tgctgtagct gcacttacag aagaaagttt ttctcctaat ttcacgcctg
    15961 ccaaatcaac catttgctgt gctactttgt tcactttccc aggtccgatg atgacttgaa
    16021 gctgttcatc ttctactaca ccaagcactc ctggaatcgc ttttaattca gagacattta
    16081 caagatcatc attgatgatc ttcatacgca cacgagtcat gcagtggaca atgctttcta
    16141 cattgcccat gccgcctgcc cctttataaa tttgttttgc cagttcttcc aaagtcagtt
    16201 cgttttttgc catggtcttt tccccctcat aaggtccctt ttacaaatcc gtttgcttca
    16261 gctaattttg cttgagcttc ctctttatca ctatttgtca aaatcatgac gatagccagc
    16321 ttaacatttt gatctgcttc ttcaaagtac ttttcagctg tttcgtaatc agcttctgtc
    16381 gcttgcatga tgattcgttt tgagcgttcg attagttttt catttgttgg tttgacatcg
    16441 accatcaagt tattgaatac tttacctata ccaatcatac tgatagtaga aatcatattc
    16501 aagataagtt tttgtgctgt tcctgacttc aatcgagtag agcctgtcaa aaattctgga
    16561 cctgcatcga cttcgattgg gaaatccgcg tgtttactga tttcggcttg tttattgcaa
    16621 gaaatcgttc ctgtgacagc accgatcgat tttgcgtaat ctaatccacc gatcacatat
    16681 ggtgtacgac cgctagctgc gataccaata actatatcat tttttgtcaa attcagcttg
    16741 attagatctt cttgccctag ctcataagaa tcttcggccc cttcgacagc tacagtcatt
    16801 gcttcttctc caccagcaat tagacctatg accatttccg gttctacacc gaatgtcggt
    16861 acgcattcag cagcatctaa cacacctagt cttccacttg ttccggctcc catataaatc
    16921 agacgtccac cttttttaaa ggactcaatc gtgttagcaa tcactggttc gatacttgtc
    16981 agttcttttt caaccgcagc ggccactttc tgatcttctt tgttcattag ttgaacggct
    17041 tgagaaatac tcatctcatc taatccaaaa gtttcttggt ttctgcgttc cgttgttagg
    17101 ttctctaaat tgattttgct catttttatc ttccttcttt tcttataaat tgatagttca
    17161 taccagcttt gatttggttg atcaatggta gctcttcttt gataacttta gctactacat
    17221 tcactttttc atccgctggc aaatcatatt tggttaattg gatctcaccc atatagcgca
    17281 aatacttttc gttatctaaa gtaatagacc ctttcatacg cgtagctgta tttcttgctg
    17341 ggataggcgg aatctctcga aaacgtgcat ctgcgcttcg tatgacatct cttgcatcgt
    17401 cttgccgatt cgtatgcttc cctaatatat attcgtaaaa ttcttcatct acaggttccg
    17461 tatggagcaa gactgtctgt tctttttgga aagaagcaaa ttgctcctga acattttctg
    17521 acagacctgc atctccgatg taaacaatat ccgtgttact tgctaataga tctagagcgc
    17581 ctgcaagagg atggatccct cgatgttctt ccaaagttgg caatccttga tatagtgggc
    17641 ctctcaactc tgtatctcct ggtacaaaac cttggacaag gagaccttga attttgagcc
    17701 attgattttt tttctgatac cattctttgt ccagccctgt ttcgggacgt ggataataat
    17761 tatgccaagc ttcaatctgt gagaaatcag cttctgcttc tttcaattca tcgatatctt
    17821 taggcgtgat cgtgctagca ttcaaactga ttttcaattg atgtgaccag tcggcaatct
    17881 gctgattgga aatatgataa tccattctta aacctgttac accaattgct ttcaaaggcg
    17941 ttagatcttc taatgaaaaa cccgctcgtt ccaatgcctc tcctgaaata tcgaccatca
    18001 attctagttt attctttttc gcttcgcttc ctagttcgat caatcgcttt tgatatgccg
    18061 aaacatcatc ttctggtata tgcatggaag tgaaaatacc gacaaagcca ttggatgcca
    18121 tttggcggat atactgtttc ttctcttctg acaaagaatc gttcataaat attgaaaagc
    18181 caaacatatt atccctcttt ttctgtatcc gtcttcattt ctatgactag agtatatatc
    18241 taaagaaaca aaatttcaat acttttatta aaataattaa atttcatttc taaaggttga
    18301 gtttatatta tcaaaagaat tgtcataacc taaaaaaggc actgctaagc aaaagcagtg
    18361 ccttgaaata ttttatttaa ataacacaaa aacataatta ctcataagaa accaattctg
    18421 tattatctac catgaatgta aagaacccct cattaggcat cggatgaaaa taagcattga
    18481 tcactcttat caaggcatta tgacaaacta acagatagac attttcttcg tcttcgatgc
    18541 attctttcaa taaaggaacg atccgtgcat acacatctaa gactgactcc ccatttggga
    18601 atcgtacagc gaacgctaaa cgtgcttttt gaaagttctc atctttgctt ggcagtccat
    18661 cataatcccc aaaatccatt tctaccaatc gttcatcaac ggtcagcggc aagctgagac
    18721 gatcagcaac tgcttgtgct gtatctcttg ctcgttgaag aggggaatgg atgattttcg
    18781 tgattggaac aggcaaatct actacctttt ctgctagttt ctgtgcctgc atctgtcctt
    18841 tttcagtcag cggcacatct gcccgtccgc aaactaatcc ttgtgcgttc caagttgttt
    18901 ctccgtgtct cgttacataa agcatgtgat tctctccttc gttggcaaat ttccccttaa
    18961 ccataccttg ttgttcttga gattacaagg gttagattct ctcaagagaa ataatctcag
    19021 tagatacttt atccaggaag gtttgatcat gttccactag taacatggca ggtttcacgt
    19081 tcaagatcag ttgttctaat tgttcttgat tgaagacatc caaataattc aatggttcat
    19141 cccatgtata tagttcagct ggctgtgata aagatttagc caattccact tttttccgtt
    19201 ggcccatact catctgctcg atcttgttat gaaaaacatc tctttccatc ccaagctttc
    19261 ggaggttgtt caaaaatgct tggtagtcta cttggttttt ctctgcaaat tccgccaacg
    19321 ttcctcgatt gtcttcataa ttttggcgta cataactaat gctcagatgt tttggctggg
    19381 atttttctcc tataaccttg ccgttgaatg cccccagaag ataatggata atggatgact
    19441 ttcctgcacc gttaggacct gaaatcgcca cccgctgatg aggctcgatt gtaaaatgaa
    19501 ttggctcgaa taacagattt tcatacccta attgaagatc ttctacgctt aaaagtcgct
    19561 tatggtgaga cgcttggcta ttcatcgtca aagaatcgat atattcgata tcttttaata
    19621 gtttttcttt ctcgctgatc tgggtctcca tccgattcac gattgctttg gatcgtttca
    19681 tcgtccgtgc agcatcagca cccactgctc ctttattcac tcgtctagat tcagtatcga
    19741 tgaatccgac ttgtttcttt gttttatctc cttctcggga acgagaccat tcggcttttt
    19801 cagctgctgt tttctttagc ctactgactt cttttttcaa tttttcattt tgagccattt
    19861 caaattcatc acgaagtttt ttctgttctt catagataga gaaattccct tgataaagtt
    19921 ccagttgact tttttcgatt gctaaaacat ggtccactac ttcatcgata aatccccggt
    19981 catggctgac cacgatgaag ccttgttttt tctttttcaa ataagccgct acttgttttc
    20041 taccagagat atccaaatga ttcgttggtt catcgattaa cgggaaatga gtgtcatcca
    20101 caaataaaag tgccagtagg acttttgtct tctctccccc cgatagtgtg ctgaattctc
    20161 tccataagat ttcaggatct gtttgcatca attggagctc tctttcgatt tcccatatct
    20221 caaaatccgt aatatcattt aacacgtaat aggttaaacg ttctttatct tttgtctgtt
    20281 gcgggaaata ggcaaattct tgctgatgga ttacttgccc ttgataaggt agtttgtttt
    20341 gtagaatatt cagtaaagtt gtctttcctc gaccgtttcg tccgataagt cctagtttcc
    20401 attgtgtgtc aaaatttaga tttgcttgtt caaataataa tgtgccttgg ctgtcgtagc
    20461 cgaatgtcag attttttatt tcgattttag acattttcca tcacctcgta aaaaaagacc
    20521 agtcctattt tcagaactgg ctaaagagga tgcaagggtt ggatacacac aaaaagaccc
    20581 atacatcgaa aaaaccaagc caatcccgaa aatctgcaca ctgcttcctt gtctccacaa
    20641 ggtacagtct gaatgcagct ttcatcaatc gacttaattt ttcaatgcac ggatccctca
    20701 ctttgtccta tatttgaaat tacgatagca taaagaaaat gattttgtca actacttcgg
    20761 gaggatggtg aaaaatctgc taagtgtctg tcctaagatt tttgtttttt ctaatgtttg
    20821 cctgtgttcc attgcttctt ctaaacctat tggacccaaa tgaatactta agacaattgg
    20881 gagaaaatca gagagtcctt ctagctcttg gtctctactt ccgcaaatag cgattattgg
    20941 ttttcgatat ttttttgcaa gcactgctac accgtgtgga acttttccgt ggatcgtttg
    21001 ctgatcgatc cgcccttctc ctgtaatgat gacatctgct tgttcgatct ttttttccaa
    21061 tccagtgatt tggctgacta aatcgaaacc agaaaccatc ttcgctccca acaaaaaaag
    21121 ggctccacca ataccgcctg ctgctccact tcctggaata tcatctaaat ccacctccca
    21181 gaaatctttt atttgctgag caatctttcc tgcttgttta tctaagaaag ctagcgtcgt
    21241 atctattcct cctttttgtc ttccaaattg ccaagcagct ccttgttgac ctgtatatgg
    21301 attggtaaca tctgcagcga taatcaattt tactttagaa acaagaacgt ttcttcgatc
    21361 aaaatgaagt tttttagtag ataataaagg atttccttct gtgtaccctt ctaaagaagc
    21421 acccaaggat tggagtaatc ctagaccacc atcgctacac ccgcttcctc ctaaagtgac
    21481 aataatttgt tctacttctt gaagcagtgc atctttgata agttctccta aggcataact
    21541 ggaagcaagt cggatagttt gatccgttgg tttatcaagt agatctaacc ccaatgcttg
    21601 tgcagattcg atgactgcgg tattttttcc ttgccaagtt gtcaataaat agctgatttt
    21661 tcgctgacgg aataaaagat ccatcccatc aatggtacgc catgttcccc cgagcgcgta
    21721 atagattgct tctgcagtat tttctccgcc atctgcaatc ggcacattga tttttcttcc
    21781 tttacgatct tcccagccat ttagaactgc ctgatttagt tcaaatgaag aagcacttcc
    21841 tttaaatgaa tcaattgccg ccaaaatatc catctgtatc cctcactttc ttcttaagta
    21901 agacactttt tttcattttt ttcaaacaaa acaatgctat tttcttccat ttttctatat
    21961 actaaaaggg aactcattga taaaggagag aaaaaacatg aaaatcgcaa tagctggagc
    22021 cggagcaatg ggcagccgaa tcggattgat gctacatcaa agcggaaatg aggtcctctt
    22081 gatcgatcgt tggcctgcac acattgaagc cattcgtaca aatgggttga ttgctgactt
    22141 taacggtaaa gaagtggtcg caaaactgcc aatctattca ccagaagaaa tcatcgaaag
    22201 caacgaacac gtggacttga tcgtagcatt aacaaaagcg aatcaattag atgatatgtt
    22261 tcgttcgatc caatccatta ttactgacaa tacttatgtt ttgtgtttac taaatggatt
    22321 aggtcatgaa gatgtactgg aaaaatatgt gccaaagaaa aatatcttat ttgggatcac
    22381 gatgtggaca gctggtctag ctggtcccgg aaaagtcaca ttgttaggtg atggagaaat
    22441 cgaactagag aacttggaac cagaaggcga agcatttaca aaaaaagttg tggaagtctt
    22501 tcaagaagca aaattaaatc cgatctatag ccataatgtt cgttattcca tttggcgaaa
    22561 agcttgtgta aatggaacat tgaatggttt atgcacgatt ttggattgta atattgctga
    22621 actaggcgcg caaaaaacgg cagagagcat ggttcgaaca atcgtatctg aatttgcttc
    22681 tatcgcagca aaagaaggaa tcatattaga tcaagaagaa gtgtaccaac atattgcttc
    22741 tacttataat cctgacaaca tcgggttgca ttacccatct atgtaccaag acctgatcaa
    22801 aaaccatcgt ttgactgaaa tcgactacat caatggtgcc gtttggcgaa aaggacaaaa
    22861 atatgatatt gctacaccat attgtgcctt tttgacccaa ttagtccatg caaaagaagg
    22921 aatccttggc gcagaataaa aggctctctg atgagaatca gctgaatcag cagctggttc
    22981 tcttttttta agcccaatga aagatcaaaa agaaaacaca actctcatga tgccataaga
    23041 attgtgcttg tttgtttcgg tacataatct cttcaaaatg taggtttttt atttattttc
    23101 aacagaggag aggtcatatt cgtattgctc ataaatagtg gaatcgttag tgataaatcg
    23161 ttttgcccaa ttttctgcat cataaaattc caatagctct gatgggattg gcttaaaagg
    23221 tgtatgcaga aattccggta attcatctaa atcacgttct cgaagcaaaa aaatattttc
    23281 aggacgataa aaatgataat gagcaatcgc ttgattatcc gtcactagtt tcttttgaaa
    23341 atacatagat tcaaatgtcc gcaaagtcac tccttgctga ccctcccgca acagttccag
    23401 aatcccgcgc gattcccctg tttttttcag gtactcttca taagcaagat atccaggttg
    23461 ttcattccct cgatgtggaa gaatgaaata gttggtcgta acatctcttt ttataaattc
    23521 ctgcttatag ctttctgctc gttcttttct cccattattc gttgcaccaa aaaatagatc
    23581 cgtctttggt tcgctagaag aaaaatccat atgtttcgaa taaaatgaac tattatgttt
    23641 cagcccaagt tcttttgctt ccaaaaaatc aaaatgataa atctcatcga ttgccggttc
    23701 atcttgtgcc tgcttcagca tcgcctcgtg ttctgctttg aagtgattcc aaaaaaataa
    23761 cacaattttt gcattggtaa atgggcgcac gcgtcgagcc atctccatat taaatccata
    23821 atctaaaaaa acgatcagct gataatttgc caactgttgt ctagctgatt taatgaattc
    23881 cgactgtagt aaatcttttc cggaccaaac atctaaagag cggatggaag gcaaaaaata
    23941 aagtgattta ttttttgcaa ctagcagtac gtcatttttg ttcatctttt ccgcctcact
    24001 ttctctgtca ctttttccta tgatagcctg ttt
//
LOCUS       DAKBVG010000014        23715 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000014 DAKBVG010000000
VERSION     DAKBVG010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 23715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23715
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.014"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            <1..217
                     /locus_tag="OUD09_000467"
     CDS             <1..217
                     /locus_tag="OUD09_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9691766.1"
                     /translation="QDIFFVLINKNDKNQGFLQFYGAAYAVLGVMGILTAFFNQRFIA
                     LVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            290..769
                     /locus_tag="OUD09_000468"
     CDS             290..769
                     /locus_tag="OUD09_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCT9691767.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            992..1135
                     /locus_tag="OUD09_000469"
     CDS             992..1135
                     /locus_tag="OUD09_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HCT9691768.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            1713..3296
                     /locus_tag="OUD09_000470"
     CDS             1713..3296
                     /locus_tag="OUD09_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCT9691769.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(3733..4917)
                     /locus_tag="OUD09_000471"
     CDS             complement(3733..4917)
                     /locus_tag="OUD09_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCT9691770.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(4942..5949)
                     /locus_tag="OUD09_000472"
     CDS             complement(4942..5949)
                     /locus_tag="OUD09_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCT9691771.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            complement(6121..6480)
                     /locus_tag="OUD09_000473"
     CDS             complement(6121..6480)
                     /locus_tag="OUD09_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9691772.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(6452..6823)
                     /locus_tag="OUD09_000474"
     CDS             complement(6452..6823)
                     /locus_tag="OUD09_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HCT9691773.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            complement(6840..7142)
                     /locus_tag="OUD09_000475"
     CDS             complement(6840..7142)
                     /locus_tag="OUD09_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9691774.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            complement(7159..7629)
                     /locus_tag="OUD09_000476"
     CDS             complement(7159..7629)
                     /locus_tag="OUD09_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HCT9691775.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(7604..7882)
                     /locus_tag="OUD09_000477"
     CDS             complement(7604..7882)
                     /locus_tag="OUD09_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCT9691776.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(7879..8928)
                     /locus_tag="OUD09_000478"
     CDS             complement(7879..8928)
                     /locus_tag="OUD09_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HCT9691777.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(8885..9814)
                     /gene="tadA"
                     /locus_tag="OUD09_000479"
     CDS             complement(8885..9814)
                     /gene="tadA"
                     /locus_tag="OUD09_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HCT9691778.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            9946..11700
                     /gene="ade"
                     /locus_tag="OUD09_000480"
     CDS             9946..11700
                     /gene="ade"
                     /locus_tag="OUD09_000480"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HCT9691779.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(11798..12460)
                     /gene="trmB"
                     /locus_tag="OUD09_000481"
     CDS             complement(11798..12460)
                     /gene="trmB"
                     /locus_tag="OUD09_000481"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCT9691780.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12530..13312)
                     /locus_tag="OUD09_000482"
     CDS             complement(12530..13312)
                     /locus_tag="OUD09_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HCT9691781.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(13516..14727)
                     /locus_tag="OUD09_000483"
     CDS             complement(13516..14727)
                     /locus_tag="OUD09_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT9691782.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(14720..15457)
                     /locus_tag="OUD09_000484"
     CDS             complement(14720..15457)
                     /locus_tag="OUD09_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT9691783.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            15595..16029
                     /locus_tag="OUD09_000485"
     CDS             15595..16029
                     /locus_tag="OUD09_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HCT9691784.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            16022..16366
                     /locus_tag="OUD09_000486"
     CDS             16022..16366
                     /locus_tag="OUD09_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCT9691785.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            16546..17556
                     /locus_tag="OUD09_000487"
     CDS             16546..17556
                     /locus_tag="OUD09_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCT9691786.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(17625..18569)
                     /locus_tag="OUD09_000488"
     CDS             complement(17625..18569)
                     /locus_tag="OUD09_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCT9691787.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(18553..21273)
                     /locus_tag="OUD09_000489"
     CDS             complement(18553..21273)
                     /locus_tag="OUD09_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCT9691788.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            complement(21270..22490)
                     /locus_tag="OUD09_000490"
     CDS             complement(21270..22490)
                     /locus_tag="OUD09_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HCT9691789.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            complement(22771..23616)
                     /locus_tag="OUD09_000491"
     CDS             complement(22771..23616)
                     /locus_tag="OUD09_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCT9691790.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
BASE COUNT         7307 a         4776 c         4066 g         7566 t
ORIGIN      
        1 acaggatatc ttttttgtat tgatcaacaa aaatgataaa aatcaaggat tcctacaatt
       61 ttacggagct gcatatgctg ttttaggcgt aatgggtatt ttaacagctt ttttcaatca
      121 acgctttatt gccttggtct ttttactgat cgtaattatt gtttctgcca ccttcagtat
      181 ccgatttgca aaaaaaattg ctgaaccaaa gcagtaaata attctctttt tccctaaagt
      241 gttctacaat aaaagagtac aatacgattg acttgaggag tgaatgctta tgttacaaca
      301 atacaaaaaa atcatggttg ccgtagatgg ttctgatgaa gctgaattag cttttaaaaa
      361 ggcggtcaat gtagctatcc gaaataacgg agaacttcta ttagcacatg tcattgacac
      421 acgttctttc caaaccgttt catcctttga tggaatgctt gcagaacaag ctactgaaat
      481 ggcaaagcaa acattggcag attatgaaag caatgcgaag aaagctggct tgaataatgt
      541 aacctctgtc gtagaatatg gctcaccaaa acaaattatt gccagagaaa tcccagaaga
      601 taatcaagta gatttgatta tgttaggagc cacaggattg aatgctgtag aacgtttatt
      661 tattggttct gtttctgaat atgttattag aaacgcagct tgcgatgtgc ttgttgtccg
      721 aactgattta gaaaaccaat tgccagctaa agaccacacc aaagcttaac acgtagacga
      781 cctgccaact atcggcaggt ttttttgcac aaaaaaccgt gataaaaaca aaagacgcgg
      841 caagcactca tcggtcttta taatgtttaa aataaaggga aaacagtcaa tgagttcaag
      901 taaaataaaa aaaagtgaac accagggcga ttagtcgccc tggtgtggtt taaaacgacc
      961 taagcatgcc aaagcttatt ggttcgtcaa tgtggaagtt tccgatgaat ctgatacgaa
     1021 aaaatatgaa gtatcgatgg cggataccga ttttatcgcc aaactaaaat tttatttcgg
     1081 gttcgggtac cgcgagaagg caagtgctac attcggtaaa tcattcaaga attgatgaaa
     1141 ggctaccgct tgccattgca accacaacga tactcctgaa cgcgatgttg gtgagatttc
     1201 caaagagaag agctgtttaa tgcaacggta ttaagcaaat ggcaaaagga agtcatgtag
     1261 acaaagtttt ttatcaaaca ccatatgatt tcgcctgaac gctggtttag ctcagaatac
     1321 aatgatgtgg tccacgagac attaccctca gtcagataaa aaaagctctc tgacagaatt
     1381 acacatcgat aaatattaag taaatgtcag cgctagttga actttctgac actttcaggt
     1441 acactggaaa agacaactac acgaaggagg gaaaatacta tgatgcgcat gactaaaata
     1501 acaagtaatg aaaattacca acgataccat atcgcattta gccatgcaat tggttttaca
     1561 gttgctgttg acgggacaag tttgtccaaa acagttgaat tcacgtaaaa tcaatggcca
     1621 tctcaaggta tttttctcat gataatctga gtttaaataa cacaggccat ggataaccat
     1681 ggcctttttg agtggtctca aggagagaga tcatgatcat acaagcaatt tctattaaaa
     1741 aaacaatcgg cgggaatcca ttatttgagg atttatcctg tcaaattgaa gctggtgaaa
     1801 aaattgcctt agtcggtatc aacggaactg gcaaatcaac ctttttacaa atcttgaccg
     1861 gcagagaagg tgtcgacagc ggtgttatca gtcgcaaaaa aggcttaaag atcgggacgg
     1921 tcgaacaaga acttacagtt tccgaagcga ccgtcaacca ctacttgctc cattctgccg
     1981 ctgagataca ggacctcaaa cagcaaatga gtcgctacga agcattgatg acagaaccag
     2041 atacaaactt agagaagtgt ctggcccgtt acggtgagct gcagcatcgc tttgaagaac
     2101 taggcggtta cgtattagaa gaccggattg cagcgatctt gcaaggctta gggattccac
     2161 accgtcagga agcacggtta acagaactta gcggaggcga acgagttaaa gtcgctttag
     2221 ccaaaatttt agcagccgat gccgatttac ttttactcga tgaaccgacc aatcatttag
     2281 atcttaacag tattcgctgg ttggaaaact atttacagaa tacaaaaaaa tcggttctgg
     2341 tcgtttctca tgatcggcaa tttctcgatc aagtcaccac aaaaacatgg gaactggaag
     2401 aaggtgcatt gattgagtat cccggaaact acagccgttt tcgagtcttg aaagaagctc
     2461 gcttggctga gctgacaaaa aattacgaac tgcagcaaaa agaagtccag cggttgaaag
     2521 tcatgattcg tcgttttcgt cagtgggcac atgaaggaga caatgagtcc ttcttcaaaa
     2581 aagccaaaga actggaacgc cgcctggcga aactgacact cgtcaaaccg ccacctcccc
     2641 cgaaaaatcg gctgcaaagc ttgtctaatg gtggaaaatc tggcaaagaa gttttcatca
     2701 ttcaaaactt gcaccagcag tacgctgatc aagtactatt caaagacagc agttttgcag
     2761 tctatcgtgg agatcattta gccatcatcg gcgacaatgg tgcaggaaaa tcaaccttac
     2821 tcaaattatt actcggtgag gaaaagcctt acggtggcac catcaaatta ggtagtagct
     2881 tgcaaatcgg ttatttgccg cagcagctgc agtttgccga tcccgatagc cgtttgctgg
     2941 cttacgccat cacattgact ggaaacgaag aagtcgctcg acggcaatta gccaaaagtg
     3001 gcttttatca aagcgatgtg ggtaaacgga tcaaagattt atccggtggt gaaaaaatcc
     3061 gtttggcgtt gctgaaactc tttttagaaa aaatcaacgt cctgattttg gatgaaccaa
     3121 ccaatcacct cgacagctat gctcgagaag aaattgaaga aatgctgcaa gactatcaag
     3181 gaacgctcct agcagtcagc catgaccgct actttttgca gcaacacttc caagaagcac
     3241 tcgttattga tcaggagcag atcagccgcc agccgctcgg ccaactgatt gactaggacg
     3301 ttttttcgca acaaaaaact tgattttcat acctgtgctt tcacgattca ggcaaagtac
     3361 aggtggaaaa tcaagttttt attattcgat caatgaatgt ttcggatcgg ttttctagct
     3421 aaacataaag cgaagaataa agtcgacagc ccacgaggct gatctaaaaa tgttttttct
     3481 ttttacatat gtaatgtccc ttataaagat gaatgaaaat aaaatattgg gattttcctg
     3541 aaaatcaata aataaaatgc cagtcttttc ccttttgacc acttttccaa gcaaaattcc
     3601 ctaagaagat cctgtaaaaa agaagggaaa ttgaagttaa attaccattt tgcggtcttc
     3661 tccgcaaaaa aaacacatgc aagaattccc tcttgcatgc gtttcatctt ttatcctaac
     3721 gaatgctttg gcttagccgc gcagacgctc cacatcacgg gcaatcatta gttcctcatc
     3781 tgttgggatc actaatactc ttacttttga ttcttttgta gagatatcta cttcttcacc
     3841 acgcaattca ttcaacgctg gatcgatttc acaaccaaac caagacatac ctttgatgat
     3901 ttcactacgt acatgtccgt cattctcgcc gataccagca gtaaagacaa ttgcatcaac
     3961 accattcatc gttgtcacat aaccgccgat gtattttcgg atgcggtcca cgaaaatgtc
     4021 ataagctaaa cgaatatgtt ctttttcata gttcttttca agatcacgca tatcactaga
     4081 caatcctgtt aggcctagca gaccagattt cttgttcaaa acattgatca tttcatcaat
     4141 atcaatctct aatttttcca ttaaatatgg gagaacagct ggatcgatat cacctgaacg
     4201 tgtacccatt gtcaccccag ctaatggtgt gaatcccata gaagtatcca tggatttacc
     4261 tccatcgact gctgtgatcg atacaccatt tcctaagtgg caagtgatga tttttagttc
     4321 ttcgatcggt ttgttcaata attttgccgc acgttccgac acataacgat gacttgtgcc
     4381 atggaagcca tattttctta ctttgaaatc ttcataataa gacaccggta agctatataa
     4441 ataattttgc tttggcattg ttgagtggaa agatgtatcg aatacagcca cactcaaaat
     4501 gtcaggtaaa atctttttaa acgctttgat ccccattaag tttgcaggat tatgcaaagg
     4561 agcaaattct gctaattcag caatgcggtc cattactttc tcatcgatca ctactgaatc
     4621 cccataagtt tctcctccgt gaacaacacg gtgccctact ccggtgattt catcataaga
     4681 gcttaaaatt tgcagttcaa tcaactgatc taacagcatt tttactgcta cttcgtggtt
     4741 ttcgatatcc atgatttgtt cgaatttttg attatcgccg tacttgatgg taaaaataga
     4801 atcttgtaaa ccgatacgtt cgacgatccc tttcgcaact acggtttctt ctggcatttg
     4861 atataattgc catttcaaac ttgaactccc agcattgatt gcaattgttt tagacatatg
     4921 actctccttc tcgttgaatg attacagagc agatgatttc cagtttttaa actcgttgaa
     4981 aaattccgtc accttctctg gttcttttaa tgaagcaagt ttcacaagca agacttcttt
     5041 tacttgttgt gcttgtggtc cttttttctg taagatcaaa atactttttt gcgattgttt
     5101 attacggaac aattcatctg gcaattgaat cattccttga agataacctt cttctttaaa
     5161 ccattttttg atttcttcac tttgatccgt ttctaaaaat ccactcggca tcaaaaacaa
     5221 accaaaacca tccggcttca catatttcat tgattgctct agcaataaat gatgtgcata
     5281 actatggcct tcttcggtac ttgttaaaaa ctcttttgct ttttcgtcat tcggataata
     5341 accgattggc aaatcgctga ttgcaaaatc gacaggatct attaataatt cctgcaaccc
     5401 gtcttgatga aagtattgaa catttgcttg tgtcaaatca cttgtagaag ctgcgacagc
     5461 caacaatgta tcatcaatat ccacaccaat tccttctgtt tggtaccccg cattcgatag
     5521 gtttaaaaga actgttaata acaagttgcc cattcctgcc gcaatatcca gaatcttaac
     5581 cggtgctttt ttatttgtgt atagttgttc aataagaaaa acaaataaaa aaccgatact
     5641 atcaggagtg agttgatggt tcgcctgcaa atgttctgtc tggctgccct tcaataaaag
     5701 taattgagac agtctgcgcc attcttctgg ctcgaaagaa agtttcttca actcttcata
     5761 aagagctgtg atccgttgcg ttgtctcttt tgtcggcact ccatccacca cacgtacttg
     5821 ataatcatct attaaatttt ctgcattttc gacataggct tctaaaaagg aagttcccaa
     5881 tgcattctgt aaaagctgaa tagcttccag gttttgatta aacccttgtt ccattttttc
     5941 tggtaacatg gtgcacctca tttctagata tattataaca attttttcac ctgtttaatt
     6001 ctatcgaaca attgacaaaa tttcaatcgt tttcactgtt ttttatttgt gaaacggcaa
     6061 acattttttt attaaaaaca gtgttataac agaaaagagg gcgttttcac gaacattcca
     6121 tcattctgta ctagtatctg tatcattttt tacttcttct gtgaaccgaa agataaacgg
     6181 tgagacttcc acctctaaga atagggactg ttgtacccac tgatatgtta cagatccagt
     6241 tgtgtagtaa aaaactcctt ccgaactttc ggcaagcgta gactgttccg ataaagccat
     6301 ctgcttcatt atttttgcta tataataatt tttgattcgt atattaagat ccattgacaa
     6361 acggtagctt tctaatgctg ccatcaataa aaacgtacat aaaagaaata aaagcaagac
     6421 agaaaataaa atagagccct tataacattt tctactgatc gttttttgtc catatggcat
     6481 atccatcttc tccattttca aagtgtacat aaaaagaaat tgtgtgttct ttttccaata
     6541 attgaagctc agtgatactt gttaacatag gttgatgtcc gccggaagtt cttcttttac
     6601 gtatcattgc ctgatatctt tcgatgatat actgtttatc ctcgatcgtg tagtgcagtt
     6661 gctgattttt cacagagaca ttgtgactgc tcttgagttc ttcttctaat tgtgctaaaa
     6721 atattagcca ctctttttga tctttacgct gcaaaaattc acttacttgg atggtttgct
     6781 gaatcaaaag attgaatgtg agcaatagac tgctcaatat catcagtcca attagacatt
     6841 caatcagcgt aaatgctgga attacttgtt ttttcaatac tgatcgcttc tttctccata
     6901 ttgacaaaca ctttttcgat tactccatta ttatcgacaa tttggatatc gtatgggatc
     6961 atttcttccc ttcgctctgg tcttttgtga tacatccgct gcgtcttgat atcttcataa
     7021 atcattcgaa taaatagcag ttcctcccca cttttctttt ctgcctgaag caaaaacagc
     7081 tggccaaagc tcactaagta gacacagaca gaagcgatca taagcgctgt aagggactcc
     7141 aacaaaacaa atcctgtttt attcaattct ttttgtatag cgaccactcc ctatctggaa
     7201 ttgatacgta atcgtccgct ctttttcttc ccaataaaat tgataagctt ttagtgaact
     7261 ttcattcccg gttctagcag caaaagtaat agaagcatgg cgtttcaagg taatctcatc
     7321 aggaatatct aatacttccc aatttatttt atctggattc ggaacatgaa aaatgatttg
     7381 attctcttca ctattccaat aaaagcctgt ttggatctga ttcacaatag cgattttttg
     7441 cgtagaatag attcgttttt caaattggcc gaaaaattga tagactgcca attcattctt
     7501 ccatgcagaa aatgcgagaa ctggaaaaga aaggatcgtt gtaagtaata aaatcagtaa
     7561 gagtgattca aacaacgtat atcctgatgc taaaacctta ccttcaaggc cgctgataga
     7621 tttctgcatg ttctcgtttg atatagcctt cattcaacaa ttcctccaca gtaggaactc
     7681 ttccattatt ttgaagcgca tagagctctt tttgtgtgtc taccaactgg atgatcgctt
     7741 cacgactttc ttggtctaca tgattcctat aacgcgacaa attagggacg aacaacaaca
     7801 ctaatacact gatgatcagt aagacgatta gcatttcgat caatgtaaaa ccgctataat
     7861 aagatatttt tctctttttc atggcaaaac ctcctctatg cttccgtaaa caggtaataa
     7921 aacagcggca taaactaata aaatcaaagc cgctacgccc aaaaaaatga ttggctgaat
     7981 aaaatgcatc cagtgatgaa gacgtttgaa aagctgtttt cttgtccatt cgctgtaaaa
     8041 aatcagttct ttacctaaac ttccctttgc ctctccctgt aaaacaattt tactgaattc
     8101 ttctgataga aaacgataat tctggaactg ctcagccaat gacatacctg cctccaatcc
     8161 ttgaatcaac ttagaggcaa gatgctgaat cagactttct tgtttcgttt cctttaagca
     8221 gactatgatt tgccttaatt cgaaaccttc atgaaaaagc tttccaatct ctagtgacaa
     8281 ataagccgat tgatacaagg aatatatcgg tccaatcaat aattgtctgc ttatccattc
     8341 actctgatca acaggagaca acttcatcag acgatacctt actagtaaag ccaatagagt
     8401 aaccgctagg atgaatagta gaacatacca atgaaagact tgtatcagct gaatacccca
     8461 atgctcttgc atgaccattc ctgaaccaat aagctgaggc aaaacgaaca accgcatact
     8521 aaacaagata cctagcaaaa aggtgagcaa tagcatcgga tatgcaagca gctttttcaa
     8581 atcattgcga aatccttcaa caagccgaaa ctgtttggca atttccaaca aagttgtcgg
     8641 cagattccca tgacgttcag ctaactcgat ctgtaccagt tgctctgctc catatcctat
     8701 ttgcgaaagt acctcactaa acgcttgccc ttcctttagc tgatgttgga tatttgccaa
     8761 aataacaggt ggaaacgaac ggatcgtgag taataaataa agggattctt gcagactaaa
     8821 accagataaa agcagttctc ccatggtttg tgcaaactgg ttttgctggc ttcttgttaa
     8881 tcctttagaa tagttctgac tgccaaatat tttcctgagc ttcttcataa ctttcctccc
     8941 atgatttttt tactgattct tcctccataa attgataact ccataaaaca gaaggatggt
     9001 gattggcatc ttccaaaagt tcttgataaa tgacaccagc taaacattca gatagctctt
     9061 cttttttctt tgtcagttca tggattctga ccaatgtacc ttcaagattg cgtgcatgta
     9121 cggttgcaaa aacccggtgc cctgttagtg ctgctcttac cgcagcatgt gcagttgatt
     9181 catctcttat ctcgccaatg attagcaagt ctggacgatg ccgtaatgcg agtctcaaaa
     9241 gtgtatcata agtctgtgct attttcggat tgatctgtag ctgcagaaaa aacggttctt
     9301 ctatctctac aggatcttcg atagtaatca cttgcaattt ttcttccaaa gccaaccgat
     9361 acattagaga agtcttgcct gatccgactg gtccgctgaa aaggtataat ccacgtctga
     9421 cggttttctg taaaatagtt gcaaaatctc gagaactaaa gcaacgatgg ggtgtttcaa
     9481 aagtaccgta taatagccga atcactaaac tctctcgttg aaggtaatct cctacagaag
     9541 acaaccgcag acgaacttcc tgtttttcta aagaataagt aaatgcacct aactgagctt
     9601 ttcttttttc tccgatatcc atctgtccca aatacttaaa tcttgagatc agttgctgtc
     9661 catctgccga agagactttc ttccagaatg ttcgtttttt tccatagcga aaatagatgt
     9721 aaaatacttc tgaagctggc aatatatata cgtcttgggc atgtttggca aaactttcac
     9781 ttatcatttg atccgataat cgttttattt ccattattct cccctccaca aaaaacatac
     9841 gtctttttga tctttttgtt tcaaataaaa tgaatttaca aaaaaatatt ggcaaaaacc
     9901 gaatttatgt tatacttata aaaatttatc ttgggaggca ctccattgga aacaacatta
     9961 aggaaatata tcgaagtagc aagtaaaaaa aagcctgctg acctagtgat caagcatgct
    10021 aagatcgtca acgtctttac gaaagaaatc atggaaaaag atgtagcaat ctgcgaagga
    10081 atgatcgtag gaatcggtga atacgaagga aaaagcgtac ataatgctaa tgggcaatat
    10141 ctagtgccag gattcatcga tggccatgtg catatcgaaa gctcactttt atcaccaagc
    10201 gaattttcaa aagtatcctt gcttcatgga gttactactg tagtgacaga tccccatgaa
    10261 ataggaaaca ttgctgggag tacaggtctt gagtttatga tagaagatgc aagacaaaca
    10321 ctcatgaata tcttttttat gctaccttct tgtgtacctg taaccccttt tgagaccaat
    10381 ggtgcaaatt taaatgcaaa aaaattagcc ccttttttac aaaaacccga agttttaggc
    10441 ttagctgaag tcatgaacta tcaagcagta gcaaataatg aaacagacat cctagaaaag
    10501 atccaattga tgcaccagca taagaaaaaa attgacgggc atgcagctgg tatcggcatg
    10561 gaagagttaa atgtttatcc tgcagcaggt attcggacgg atcatgaagc tacaactgca
    10621 aaagaagcta aagaacgttt ggatcttggt atgtatttaa tggttcgaga agggaccgtt
    10681 gcaaaagatc tggcatcgct actaccggcg atcacacctg aaaattcaag aagatgcttc
    10741 tttgtcaccg atgataaact gatcaatgat ttagtaaaag aaggatcaat cgatcatatc
    10801 gtccgtcaag ctattgcact tggaatcgat ccattacaag ccattcagat ggcaacattg
    10861 aatgcagcag aatgttttga tttgaaaaca ctaggtgcga ttgcccctgg gtatcaagct
    10921 gatttcttct taacagacga tcttaccaag ctgccaattc atgaagtttt ctataaggga
    10981 acattagttg ttcaatctgg gcaactccaa gaccatttat ttgctgatca ttcaaattca
    11041 tatacgtgtt gtctgccaaa actaaacgct caaccattaa atccacattc gttagagctg
    11101 cttctttctt cttcacaagc ccatgtgatc gaaattatcc cgaatagtct agtgactaaa
    11161 gatttggtag aaaaagtgga ccgacaaggc gaacgattca ttccttcaat cgaaaaagac
    11221 caattaaaga ttgcggtgat cgagcgtcac catcaaacgg gaaacattgg attaggcata
    11281 gtgaaaggat ttcagctgaa acaaggagcg atagcgacaa cgattgccca tgactcgcat
    11341 aatctagttg tcgttgggac aaacgacgag gatatgctgt atgctgctaa tcttttgatt
    11401 caaaaaggtg gcgggatgac cgttgtcaac aaacagcagg aaatagcttg tgtttctctt
    11461 cctataggcg gaatcatgac acaggaacct ttttcgatcg tctatgaaca gttgaatgaa
    11521 ttaataacta aagcacatca actaggagca gctaaaacct ttgatccttt tttgacactt
    11581 tctttcttaa cattatctgt catacccgaa ttgaagatca ccgacaaagg attattttca
    11641 ttttctcgct tccaattgat tccaccttgt ggaaaaaccg atgaaataaa tacattttaa
    11701 atcaaataaa tcccagaaaa tacattacat ctgtaatgtt cattttctgg gatttattta
    11761 tggatattaa tgtttttatg aaagcagaaa gttagtttca cagacttttc tttttggtct
    11821 tatcttgaaa ttgagcttct actcgataaa tacgttgacc ttttgaagaa aatttttctt
    11881 catactccgt cataatattg ccttcaaatt gactttgatg taaatctaac catacctgtt
    11941 tgaggatcat gccatattga gaaaaacttg ccagagaata ctcaaacaat ccttgattat
    12001 ctgttttgaa atggatttct ccatttggac gaagaatctg ttcatctaca gcaagaaaag
    12061 tcttatatgt caatcgccgt ttttcatgac gtttttttgg ccatggatcg gaaaaattca
    12121 gatagatttg atctacttcg ctgtcagcga aatactctgt caacgctgat ccatccacat
    12181 gtagaagttt caggttagga agcggttgtt cgatcaactt gtctagtgca atcgaaacaa
    12241 cactgacctg catttctact ccaatataat tgatttcagg atgggctttt gccattccag
    12301 tgatgaactg gcctttcccc ataccaatct caatgtggat tggatgatcg ttttcaaaca
    12361 attcattcca ttttcccttc cacaatgttg gatcactaat tacaaaattc gggtgagccg
    12421 ctaacatttc cgctgctccc ggacgatttc ggacacgcat tatttatctc cattcctttc
    12481 ctctaattaa aaaggtctgg ccaaatctcc gcagagtttg ggccaaacct cagccactga
    12541 aaattcgttt taattgtaaa atttcttcgt tcatccgtcg atgatcttca cgtttgcaat
    12601 atctggtgat ttcttggagc aaattcatgg ccgcatacca atgaaatttt tcaagcattt
    12661 cgtcagtcgg gcgtatgcca taactgatca gccactgatt ccagctagac agtggcacat
    12721 aatttcctaa aatcgttgca atatccaccg cagggtctgc aaacatgatt gaatcccaat
    12781 cgactagata taaataattt ttacatacta accaattacg atggtttaca tctccgtgta
    12841 ctactgataa gttttcctta tctattgacg ggatctgctc acgaagaaaa cgatggacaa
    12901 tttgcagaaa ttgatttttc tggagttctt ccggtaattc tttttcatat ttttgcagca
    12961 tcttctccgg cgtcatgaca cgaccaccga tacgggtcag catggtttta agagaatttg
    13021 aatgatgtaa atggtacaaa acatcaatca cgtcgtttct ttgaccaatc tcattaggag
    13081 tcagcaagcg accttccagc cactcttgtg ctgtcaaagt atcgccattc cccgttcgtt
    13141 ttgtccaaac aagttttggt gtaattcctt cccttgaaag agcagccaac atcggagtcg
    13201 tgtttcgttt aataaagact ttgtcctcat cacgcatgcc tatgtatgtt tttcctgtgt
    13261 cgcctttgat gggacgtaac cgccactctt ggtctaactg aaactccatc atgcattctc
    13321 ccgtcctctt actctttgtt ttgcagtact tattattcta gtcgcctgaa cactacacgt
    13381 caagcacata tacgttcttt ttcatttaat tgccattcaa aaaaccagct tttctcaaaa
    13441 aaaatagaac gaacagcatt cttactgtca acgctctatt tttcattctc atttttaccc
    13501 tacacagctt tgttttcagt ctgacatctt cttcaaacga gttggtacat atagtttaac
    13561 aaagagcata gtaacaatca aataagccgc tagcggaacc catcgctgct gcccatctaa
    13621 tacgactgca gcaatcaaac caaaaataac cgacgcgata attaataccc atgtgagtag
    13681 atactgaatc gcctgcttct tctgattctt atggattgga taaagctgaa cgagaaccat
    13741 atagcggaac tggttcgcta aaggtaacag ttggaaaccg atcaaatata aaaataagaa
    13801 accgataatc agactaaaat aaaagtcgac tactccggca atcaataacc cacctataat
    13861 tactaatcgg aaataaagac cactaaaatc agaacctctt gccaatcttc ttgcaaacaa
    13921 gtaaagataa gtgttttttg attctctttt aatatgagtc aatataggat ccaaatagcg
    13981 tcttcttttg atctttgccg taatttcagg aacatctgtg aataaattga taaattgata
    14041 gatctgatgc aacctatgtt cttctacatg caccattttc tcccaatcca atcctttttc
    14101 ttgttgcttc cacaaaaacc agtaatatag acttgcctgt actaatgcac ctgctattcc
    14161 tgcccatatt agccaataaa atgatagaaa caaaaagatc agagaagaaa aaaaccagcc
    14221 taatttccaa taacgaataa cttgttgatc tagtcgaaac aattctgctc tcttgatttg
    14281 cagatgactg aatttcaaac tccataatat gatcagatag acgaaaaaca tcgaaaagga
    14341 ttgctccgta ctaaccacca ctaatggcat ggccaatccg catgccaata tcaaaaagat
    14401 caacggaaaa atgcaagaat agcgaaaagc tcgttccaaa taggtttgca tttctttttc
    14461 tttaggaagt aaaaaaacaa tatctgctgc ctctgcaaaa gtagccatct tacccacgaa
    14521 taaacagagc atccaaaata cagagattat gagccctcca attgcaaacg gtggctgcag
    14581 ggttttcaac caattagaat aatataatcc cacaccgcca atcaagaaaa gacaaactaa
    14641 aacaaaatga tcgttcaata cataacgcat atatttcatc attttctttt gatgtcttgc
    14701 taaacgtttt tgaaaaaaac tactcatgtg cctgctcctt tgttaaagca atatagattt
    14761 cgtccaaaga agaatccggc agctgaaatt cttttctcaa catctcaaga gaaccgtctg
    14821 ctcgtatctc tccgttatgt aagaccacaa aacgatcaca atatttttct gcagtagcta
    14881 aaatatgcgt agacatcaaa ataccggctc cttctttttt catttctacc atcaattcaa
    14941 gcaatgcatt gatagctagt ggatccaagc ctaaaaatgg ttcgtcgatg atatataaag
    15001 aaggctcgat caaaaaggcg cataagatca tcactttctg tttcattccc tttgaaaaat
    15061 tagctgggaa ccagtcaaga cgattagata accgaaaagt ctccaacaac ttttctgctc
    15121 ttttcattgc tatatccata ggtatatcgt aggccatcgc agtgacttcg atatgttctc
    15181 tcaatgtcaa ttcttcatat aaagaaggag tctctggaat atacccaatt tgtttgcgat
    15241 attcttccgt atttttcttt aaggatagat gattgatttc tatcgttcct tttgaagctt
    15301 ccagcagacc gataatgtgc ttgatggtcg tacttttccc tgctccgttc agtccaatca
    15361 accctactat ctctccaggc tgtacatcga aactgatatc ttttaacaca gggagatgtc
    15421 cgtacccccc tgtgacatga tcgataatta aactcattct ttttcctccg ccatttattt
    15481 ttatttatta taccacgaag aaaatgacgg tattttgttt ttttccttac aatagcttta
    15541 acttatggta aagttaagct gaagtattta ttctttatag aaaggatgtt ttatatggaa
    15601 aactgtattt tttgcaaaat catcaatcaa gaaatcccaa gctataaaat atatgaagac
    15661 gacaaggtct atgctttttt agacatctct caagcaacga aaggacacac attagttgtg
    15721 cctaaacgtc atgtagcaga tatttttgaa tatgatcccg aacttgcagg agaggttttt
    15781 tcgcgtgtgc ctaagattgc tcaagcattg gaaaaagctt ttccagaaat gaaaggtctt
    15841 aatatcttga ataacaaccg tgaactagcc tatcaatctg tttttcattc acatatccat
    15901 ttagtccctc gctacagcaa agaggatgat ttttctattc actttgtcaa tcatcaagat
    15961 agttatggat cggaagaact aaaagctatt caagaaacaa tcgtaaagca ggtgtcctgc
    16021 gatgattaaa aactttttca aaggactgtt atttggagct gctgctggca ctgtcggagg
    16081 tctgttattg gcgcccagga gcgggaatga aacaagaaag aaactgatcg aagaattgga
    16141 agaagcaaca actctgacaa atgatttaaa taacagcctg aatcatttta aaagcgcatt
    16201 gatcgaaaca aaagaaacag ctgaatctgt gattcctgct ttccaagaag caatcaaaaa
    16261 agatatcgaa gattttaaat tccaagccga accacgtatc acacaaatca atgaacaagt
    16321 agaaaaacta aaagctgatc tgccagagta tcctgaacaa atataataat gattattttc
    16381 tcaacagatt taactatata agtgagaaaa gtttgcgtat ttcctaggaa tgtgatgaaa
    16441 ttttgaattt tgtttgaaac taccacgaca acgtttttaa aggaaatggg atatggtata
    16501 gttaatatat aaaaataata actaattaca ggagtgcaaa ttttaatgaa aaaaaaatca
    16561 attattttgg cagctactag tgcgttggct gttttaactc tagcagcttg ttcaggcgat
    16621 actaataaag atatcgcaac gatgaaaggc ggaacgatca ctgtatctga tttctatgat
    16681 gaagcaaaac ttgaatcatc gaaccaatcc ttagtacaac ggatgatcat ttataaagta
    16741 ttcaataaca aatatggcga taaagtaacc gataagcaag tggatgcaga atacgacaaa
    16801 caagcgaaat cattaggtga tacttttgaa tcacaattag aagctgctgg atatacgaaa
    16861 gatacttata aagaatatat tcgtaacaat ttagcttttg aagctggctt gaaagctcac
    16921 gtagatatta cagatgacga tttgaaaact gcttggaaat ctttccatcc agaagtagaa
    16981 gcacaaatca tcaaattatc aagtgaagat gaagcaaaag atgtgaaaaa atcagcggat
    17041 gatggagatg atttctctaa attagccaaa gacaaatcaa ctgatacaga gacaaaagaa
    17101 gatggcggta aagttaaatt cgactcaacg acaacaacta ttcctgcaga agtaaaagaa
    17161 gcagctttca aattgaaaga cggtgagatc tccgatgtta ttactacaac aaatcctact
    17221 tcttatgcga cagaatacta cgttgtcaaa atggtgaaaa accaaaataa aggcaatgac
    17281 atggataaat acaaagatca actaaaagat atcgctactg aaactaaact aagtgacaat
    17341 gcattcacaa ctaaagtaat cggtgaagaa ttaaaagatg caaatgtaaa aatcaaagat
    17401 gatgcctttg aaaatgtgtt gagtgcattc acaacaactt catcaagtac gaaagactca
    17461 agtgaaacta cagcttcaac aaagagttct gacacaaaat caactgattc aacaaaagaa
    17521 tcttcaactg aagaaacaac tgattcatca aaataaaaga aaacaaaaaa agcgaagact
    17581 tatgtctcgc tttttttgtt ttgaatgaaa aaaataaatt tttgctataa atcttttggt
    17641 acatagaaac ttcggttgtc cataccgaaa atccgatcgg tgtattttcc tggttctgtt
    17701 tgacgaatcg aagtcaacat catctgcatc gaagcatcga tattgtcgat ttgatgtaat
    17761 atttctgctt ccattattct tggacgtaca ggtgagccat attctaataa gccatggtgt
    17821 gctaaaacca tatgacgtaa tacgagaaca tcttcgtcag cttcgctgat tttcaacgca
    17881 agacaggctt tagttatttc ttcgtcaatc aataccagat gaccgatcaa atttcctgca
    17941 agcgtatatt ctgtcgacat aggaccggaa agttcaatca cctttcctaa atcatgcaaa
    18001 atgataccag catacaacaa tgctcgattc agctcaggat attcatcggc gattgatttc
    18061 cctaagtgaa gcattgtcaa agtatgataa gctaatccac tggcaaatgc atgatggttt
    18121 ctttttgctg ccggaaactc aaaaaattgt ttttgatatt tattaagtag atagcgaaca
    18181 atacgattcc aatgcgcatt ggtgatctca aacaacaatt gattgatttc ttcttccatt
    18241 tcttctttct tcaaaggggc tctttccatg tataaactag gctctcctgg ctcatctacc
    18301 cttgttaagc gcatatgaag aatctttact tgagggtttc cttgataaac ctctctttta
    18361 ccgtttagaa aaacgactgt gccggctgta aatttattga tttcctcctc cgaagcatcc
    18421 cagtatttac catcgatagt acctgaagta tcttggaaag taaaagcaat aaattttttt
    18481 ccgtttttag cgattcttac atctgcactt ttgatcagga caaattgttc aaactgctca
    18541 tcgattacta aatcacgaat ttttttcatt atttgctcct tctaattgtt ttacgatttg
    18601 gttcatctca tggtaatagc ttaccatttc tttatcagaa gaaaaacaaa ttatttgata
    18661 cttttctcca aatttagcta aaagttcagc taattgatac tttcgctgat gatcataatg
    18721 aagccagcca tcatcaatga tgatcgggca tatcgaacgg ttagcttcta atgagaggta
    18781 agcaaatctc atcgccataa tcagctgatc tttcgtacct gtagataact catagatggg
    18841 aaattctgta tatccgtctg ttaaagtcag tgtatcattt ttcagtaata tcttctgata
    18901 atttcctttt gttaaaatac ctaaataatc acctgctgct cttaacaact ggggtaattg
    18961 tctttcactc atctctgttg aaagatcacc taagaatgtc ccggcgattt caagtgctcc
    19021 ccattcctcc agtaattcct gtaattttgc tttttgccgg ctagcttgtt gataaagttc
    19081 gtccaatgtg ccgtctcttt gcaactcttc tacttgaagt tggagtcgtt gccgattttc
    19141 acttgcttct ttttcttctt gctggagttg ggattgcttt tctttcagtt ggcgcagtct
    19201 tacagataat ttctcttttg tcagctcttc aggaaatagt ggagtaagca tttccgataa
    19261 ttcattgtat cttttcatct gctgttcggc ttgtttggct ttttgtaaaa agacaggtac
    19321 ttccgaagga taagataagc ctgcttgagt caattgttcg ctgtattctt gtatccatac
    19381 tttttgtttc tttttcaatt gattgatttc ttgcttcaat aaagtatttt gctgatagct
    19441 tctggcaaat ttcatctgtt ccatctcttg tataaaacgg gaaagcaata ccattttttc
    19501 tgaaattgtc ttatcttgta tcggtaacca ttctttaaca aactcaaatt tttggtcgat
    19561 ttgtcgtagt ttttcttgta tttcctgttc tcttttatgt ttccgctcaa atacgccata
    19621 taatttctga taatctatga cttcctgtcc atattctttc atgagtttga gcgtatccat
    19681 ctttcctaag tgccgatgat ccgcaaaacg atatacttct tgctgaagaa tctgtttttc
    19741 ttctccagtt ctcttttgtt tttcttctgc ttcagctaat tgagctaagg ctagatcaag
    19801 ttgtcctagt ttttcttgcc acattccttt tatctcagag aaatcatttt tagttttaat
    19861 ttttggttgc cttttgtaga agaaatagcc aatcatgcct gcaccaatta gtaaaaggat
    19921 cactttcaag ggcattggta caaatatacc tgcaatgatt attgctccgc cagccatcag
    19981 catatacggt ttagatgaag agactggtgc tatctctgac tgattttgta ataatgctgg
    20041 ataggtctct tccaagtcat tcacttctgc ttcaatcatc tcttgctgtt ctttagctat
    20101 ttggagacgc acagaaatct ggctttctag atcagcaatc cttctttctt cagccagaag
    20161 atgcttccat tcttcttcat ataaaaattc ttccggtaac ggatttttct gccaatgcca
    20221 cctttgttcc aaaatcttca attctgaatt tgtttctcta gtctcaattt gtaatctttg
    20281 ccattcttcg tttaattgtt ccgctgtaaa ttgaagttgc tggagatctt ttagttcctg
    20341 ctcctgctct aaataaaaat aatatctggc tgactgctga tccatcccgc tatgcttttc
    20401 cagtgttgct tctaattctt ccaaacgctc atttgtttgc tgataccgtt tggagaaaga
    20461 ctccagatct aaaagaagtt gctcatcagg aagacctgta gtgctgtttt ttttgagaga
    20521 ttgccattct tcatataaag aaaaattaag ttcttgctgt ctggctaatg aaaattgttt
    20581 gttcgcctct tttaattgtg ccaataactg ctgctgttgc gactcgtatt cctgttcttg
    20641 ttggatcaac ttgccaaaat aagcttcctg ttcttgtttt tgatgaattt tttcttgcag
    20701 ctctttccat tcatttaatt tttgattgat tagtaatttt tggccttttt tcttgaataa
    20761 ttgttgtgct ttctgataat attcttgccg cttttggaat acttgagaac tgcccgtgat
    20821 gcctaaagaa atcagcgcat catgtagctc tttttctctc aaatgatcta gttccatcaa
    20881 ctgctcttgc tggaaagtaa atacctgctg gaataactct ttcgtcaaag gtgcgatcag
    20941 tttttctagt aactcatcac ttcccacttg atcccccagc cacaccttcg attttccgcg
    21001 attttgctgc ttgtagcgct cgacagcaaa ttcaccatat acaggatgtt taagccatat
    21061 tttgccgcca taggctgagc cgtcttttgg tgtataatcc ttttttttac ggccttttga
    21121 agggaaacca aataatattg cttgaataaa ttgataaatc gttgattttc ctgcttcgtt
    21181 tcccccatag atcagctgat tgcctgaata aaagtcaaaa cttttttgac gccattttcc
    21241 aaaagcagca atctcgatcc ttaaaatctt cattcctgat cctcctcaaa taaaaaatct
    21301 tgattaagca gctgcttggc tttttcaagt gtttcttctt gatattccgg cagttcgctc
    21361 aatattctag ccgcttcttg atgactatat acttcattta aaatttgttg gaaatcttga
    21421 ggtgtctgat agtattgaaa taactgttcc atcaatttag cagaagcagc aagtggggtc
    21481 ttcgtataaa aagtattctc tatgagagaa atacgccaaa taaagaaatc atttatccta
    21541 tctgagaatt cctctaacaa atagtctaat agctctcctg attgtatccg ttccaaaaca
    21601 tctgtattga gatggtcata atcttttaat tttatctcaa ttaaactaaa tccatcctct
    21661 acttttgaaa gctgctgacg aatgtgaaca atgacctctt ttgtcgttct tgctttcctc
    21721 aatgatattt cctttgtctt ccaatagacc tctgctactt taatcggatg tatttggcaa
    21781 cttcctttac ttaattcaac tagtagaata gatgtagaag cttgttcctt tttagtatga
    21841 ccttgaggcg cacctggata aacaattgtt tgcttttcat ttaagaccgt tggcacatgg
    21901 atatgcccta aagcccaata atcgtatccc ttttcttgca tctttgaggg ttggaaagga
    21961 gcatagtttc ctttttgaga gacgccaggt tccccgtggt acaacccaat atggtaatct
    22021 gtgagttctc tagaaggaaa ttccatcact ttgtcttttt gaatccacgg ttgacgataa
    22081 ctaaatccac tgagagagac agtttctcca ctttttatcg ttattttttt tgtctctacc
    22141 gtttcggatg taaataaatg gacattttta ggaaaggaga accaataccg ctctttttga
    22201 tagaaatcat gatttccgaa tattataaat acaggtatat ggttcttttc taaccgtttc
    22261 atctgttctg aaaaatgttt ctgtattttt aatgaaggac gattttgatg aaaattatct
    22321 cctgcaagta aaacaaaatc aacgttattt attattgcct gttcaatgat atttgataaa
    22381 acggtcagat tcgtcttcaa tagtttttcc tgtacagttt tatcaagagt tgtcagtcct
    22441 tcaaatgacc gatccatatg taaatctgcc gcatggataa atttcaccat taaaaatttc
    22501 ctcctttttt tcttttatca taccattttc aatgtttttt ttcatcaata accgaaaatt
    22561 tgttcgtatt atgcaaagaa attacgataa atggccgact tcaaaaaata agtttattcg
    22621 aaaaaaagat tgtgacatga tttttgtcac aatcctaaat atctatgtta tattcgcttc
    22681 gtttgacact gaagaagtac tatttgtact ggaatttatg ctccattttt ccgtatttag
    22741 aattctatgt tctatactac cttaattagt ttatatggta agaattcttt caaatgcttg
    22801 agatactccg tctttatcat ttgaaacagt gacataatca gcttttttca tgatttcttg
    22861 atgactattt cccattgcaa aactaatatc tgcactttca aacatagata agtcatttaa
    22921 actatctccg atcgcgattc tcaaaggttt ctttaaattg tagattgatt ccaaacgatc
    22981 aaacaccaca cctttagata cattttgagg tacgatttca atatttacag gtgccgattc
    23041 agtaacagac acatttagca tctttttcaa atggttaaat atattagctt tcaattgacg
    23101 attaggaata aaaataaaaa atttgtaaat gctgatagca tccttttcta taatttcctc
    23161 aatattatta taagggattg aacaaataga tttttcatac tcaagctcat agtttagctc
    23221 tttgatagaa aattcactcc ctacctcgtg aaaaattttt ttcattttat ttttataatt
    23281 ctgagattca taacttccga gatttgtata tattttggtt ggaaatttcg ataggacaga
    23341 atcaattttt tttacatcat ttttatcgaa ggaagactca aatatctttt tttctccatc
    23401 aaatccaaca gaaccattta accctactgc tggtatcctt aaaccagatt ttttgagaat
    23461 attattaatg tcttctactt ctcttccaga acaaatgaat ggaaaacagt cttccggtaa
    23521 atgtttcatt gttcttgcat ttttttgact aatgctctgg tcagaattca aaagagtacc
    23581 gtctaaatct ataccaatta gtttcatttc atccatgtct cctatacgtt gattataaat
    23641 tcactgctta ttgtaagtct aaatttctca caaaacatat actaattata cctaatttaa
    23701 gtaagtacaa acatc
//
LOCUS       DAKBVG010000015        22916 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000015 DAKBVG010000000
VERSION     DAKBVG010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 22916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22916
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.015"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(25..1521)
                     /gene="lysS"
                     /locus_tag="OUD09_000492"
     CDS             complement(25..1521)
                     /gene="lysS"
                     /locus_tag="OUD09_000492"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCT9691791.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1632..2636)
                     /gene="dusB"
                     /locus_tag="OUD09_000493"
     CDS             complement(1632..2636)
                     /gene="dusB"
                     /locus_tag="OUD09_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCT9691792.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2637..3524)
                     /gene="hslO"
                     /locus_tag="OUD09_000494"
     CDS             complement(2637..3524)
                     /gene="hslO"
                     /locus_tag="OUD09_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCT9691793.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3741..5852)
                     /gene="ftsH"
                     /locus_tag="OUD09_000495"
     CDS             complement(3741..5852)
                     /gene="ftsH"
                     /locus_tag="OUD09_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCT9691794.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5942..6487)
                     /gene="hpt"
                     /locus_tag="OUD09_000496"
     CDS             complement(5942..6487)
                     /gene="hpt"
                     /locus_tag="OUD09_000496"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCT9691795.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6487..7932)
                     /gene="tilS"
                     /locus_tag="OUD09_000497"
     CDS             complement(6487..7932)
                     /gene="tilS"
                     /locus_tag="OUD09_000497"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCT9691796.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8052..8522)
                     /locus_tag="OUD09_000498"
     CDS             complement(8052..8522)
                     /locus_tag="OUD09_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HCT9691797.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8568..8993)
                     /locus_tag="OUD09_000499"
     CDS             complement(8568..8993)
                     /locus_tag="OUD09_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HCT9691798.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9060..9329)
                     /locus_tag="OUD09_000500"
     CDS             complement(9060..9329)
                     /locus_tag="OUD09_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HCT9691799.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9340..10935)
                     /locus_tag="OUD09_000501"
     CDS             complement(9340..10935)
                     /locus_tag="OUD09_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCT9691800.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10950..14471)
                     /gene="mfd"
                     /locus_tag="OUD09_000502"
     CDS             complement(10950..14471)
                     /gene="mfd"
                     /locus_tag="OUD09_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCT9691801.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14488..15048)
                     /locus_tag="OUD09_000503"
     CDS             complement(14488..15048)
                     /locus_tag="OUD09_000503"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCT9691802.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15401..16375
                     /locus_tag="OUD09_000504"
     CDS             15401..16375
                     /locus_tag="OUD09_000504"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HCT9691803.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16545..17945
                     /locus_tag="OUD09_000505"
     CDS             16545..17945
                     /locus_tag="OUD09_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HCT9691804.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18023..18745)
                     /locus_tag="OUD09_000506"
     CDS             complement(18023..18745)
                     /locus_tag="OUD09_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCT9691805.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18824..19330)
                     /locus_tag="OUD09_000507"
     CDS             complement(18824..19330)
                     /locus_tag="OUD09_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HCT9691806.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19453..21252
                     /locus_tag="OUD09_000508"
     CDS             19453..21252
                     /locus_tag="OUD09_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HCT9691807.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21320..22894)
                     /locus_tag="OUD09_000509"
     CDS             complement(21320..22894)
                     /locus_tag="OUD09_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCT9691808.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
BASE COUNT         6248 a         4991 c         4200 g         7477 t
ORIGIN      
        1 attccttaga cagcttttat tcgattatct cattgttggg aatagcaaaa catcccgaat
       61 tgattgtgca tcagttagca acattactaa tctgtcgatc ccgatgccta gaccaccagt
      121 tggcggcata ccgtattcta aggcttccaa gaagtcttcg tctacaccgt gagcttcgtc
      181 atttcctagt tcacgttctt tttcttgcgc ttcgaatctt tctctttgat caattggatc
      241 gttcagttct gtaaaggcat tagcaaattc gcgccccact atgaatagtt cgaatcgatc
      301 tgtgaatcga ggatcttctg gatttttctt cgctaaaggc gaaacttcga ctggatgtcc
      361 gtatacaaac gtcggttgtg tcaatgtttc ttcaacaaat gtttcaaaga attcattgat
      421 aacatgtccg acacccatag cttcggttac ttctacattg tgtttttcgg ctaaagctaa
      481 tgcttcttcc aatgtcattt cttgccagaa gtctacacca gtttgttctt tgattgcatc
      541 taccatatgg atgcgtttaa agtcactttc taagtcgaca gcttgtccat catatgtgat
      601 ttttgctgtt cctagtactt tttcagctgc gttacggata ataccttctg tcagattcat
      661 tacatcattg aaatcagtat atgctgtgta tgcttctaac atcgtaaatt ctggattatg
      721 tgtagtatca attccttcat tacggaaaac gcggccgatt tcataaactt tttccattcc
      781 gccaacaatt agtcgtttta aatgaagttc tagcgcaata cgcaaatata aatcaatgtc
      841 cagtgcatta tgatgcgtga taaatgggcg tgcggcagct ccacctgcct cattgtgcaa
      901 tactggtgtt tctacttcaa tatagccatt gccatctaaa tagcgacgga tttcgctgat
      961 aatttggctt cgtttcataa atcggtcaaa gctttcgcga ttactgatta aatccaagta
     1021 gcgttgtctg tagcgttgtt caatattcgt taagccatgg tatttatctg gcagtggacg
     1081 aagtgctttt gacaaaatcg tgatttctga agctttgata gaaacttcgc ctgtgtctgt
     1141 tttcatgatt tgtccagtta caccaaagaa atctccaagg tctgcatgtt tgaatacttc
     1201 ataggcctca tcacctactt ggtcttttct tacataaatt tgaatttggc cttcacgatc
     1261 ttgtagatgc gcaaatccag ttttcccttt tcctcggcga gtcatcatac gaccagctac
     1321 actagcggtc aatcccattt ctgctagttc ttcttttgtt cttggatcaa atagttcatg
     1381 caattcttct gagttgtgcg tgcgttcaaa tcgttttcca aatggatcga ttccatcttc
     1441 acgcaattgt tccatttttt gacgacgtac aagcatttga tcatttaaat cttctacttg
     1501 atgttgttgt tcctgtgtca caaaagttcc tccattctca tgttaatctc tttcctataa
     1561 tgccaatgaa acacaaaaaa agcaagccga acttggaatt tcggcttgaa aattcacgaa
     1621 aaacttagaa atcacaacgt ttgtttcaat agtacttttt ccgctttttc tacaaattga
     1681 tccagcaggt cattgatttc ttgctgtttt tctgtctgat tgattgctac tttcactttc
     1741 gctgctcgag gaatcccttt taaataataa gccgcatgtg ttctgaattc tctacaagct
     1801 actttttctc ctttgagatc tgctaagcgt tgtaaatgaa gtttagctgt agcaattttt
     1861 tcggctggtg ttggttcagg aatcagttct cctgtttcga gataatgctg tgtgcggtga
     1921 atcatccatg gattgcccag cgctgcacga ccaatcatca caccgtctgc accgacatag
     1981 tcaagcatcc attttgcatc ttctggcgtt ttgacatccc cgttccccat aaatgggatc
     2041 gtcaaatgtt ttttcacttc tttcaatact tcccagtccg cttttccttc atacatctga
     2101 acccgtgtac gtccatgcat agcgatggca cttgctccag ctgattgtgc tgctaatgca
     2161 ttttctacag caaagacgtg ctcttcatcc caaccaatac gcatcttgat tgttactggg
     2221 atatttactg ctgaagaaac agcttggacc atttcgtata ctttctcagg atctaaaagc
     2281 cattttgctc ctgcttctgc tttgattact ttatttactg gacagcccat attgatatct
     2341 attatatcgg cagctgtgtt ttcttcgaca aattgagcag cttcaactag actatcttta
     2401 tttccgccaa atatctgtaa acttaatgga tgttctgttt cttcaatgtg gagcatctct
     2461 aaagtttttt tgtttctgaa atgaatacct tggtcactga tcatttcaca tacgactaag
     2521 cccgcaccaa attctttgac agtgactcga aatgctgaat ttgttattcc agccatcggt
     2581 gcaacaacga cacgattagg gatttccaca ttgccaattt tccatgtgtg ttccacttaa
     2641 atccctccta aggcttcttt cttcaatcca tctaattctt cttcgctata cttgtatttg
     2701 ttattacaga aagaacaaac cgcttctgca ccgtgatctt cgtctatcat tgcttggatt
     2761 tcttctgcac ctaacgcgat aattgcttct gcaaattttt cttttgaaca gtcacatttg
     2821 aattgaacag gcattttttc caaaatgttc aattcttctg taccaagcaa tcctttcaat
     2881 atttcttctg gtgtctgtcc ttcatctaag agggtagaga ttcttggtgt cgtttttaat
     2941 tgttcttcga tcttgctgat tgtttcttca tcggcacctg gcatgatttg gatcatgaat
     3001 cctcccgccg tcttgatgct gtcgtcagta ttgaccaata cactcagtcc aacagctgaa
     3061 gggatttgtt ctgagatagc aagatagtaa gtaaaatctt cagcgatttc tccagaaaca
     3121 atcggcgttt gcccagaaaa aggttctttt aatcctaagt cttttgtgac agtaaacatt
     3181 ccttgtgttc caactgctcc ccgcacatcg attttacctt cactatttag aggaagactg
     3241 agatgcggat ttttgatgta acctttcaca tctccctttc catttgcatc tactacaatt
     3301 cctgttgctg gaccatcgcc ttggattcga acagttagtt tgtcttctcc tttgagcgtc
     3361 gcaccaagta gcagactacc gaccaatgtc cgtcccaaag cagctgagga ggtgtgccaa
     3421 gtatcatgac gtctttgtgc ctccgctacg gtatttgttg cttgaacggc gtatgctcgg
     3481 acgaatccgt cgtgagcaag tgccttaacc aaataatctt tcatttgtta tgtactcctt
     3541 tttccgtttt taaactcata gtgaatcata ctagcaacta ctgctttttt caactttgga
     3601 atgcttcttt taaaaataat tcagaatcat ctagaaaaca ggacatcacc ctatttacaa
     3661 cttaaataaa gaaatctccc gaaaaccggt ttttgttccg gttttcggga gtcatatcag
     3721 atcaattgac ctatattttt ttatttgtat tgatcgtctg aatgattatc ttctgattgt
     3781 tgagaatctg aatcatgttc tgctttattt tcctcattca gttcatgttt tgcttcttca
     3841 aaatcacgtt tttcttccgc ttgtttttca gcgtcttttt cttccaaagc acgttttgct
     3901 tcttcaaaag ttttcgcttc tttttcgctt ggataatcat ttgactcagt accttcaggc
     3961 atcacaccgt gttcaaacaa tgacttgatt gctttggcat ccaatgtttc gaattccaaa
     4021 agtttttcag caatcagttt atgctgtgca cgatgagctt cgatgatttc tcgagcttta
     4081 tcatgagctt ccatcaagat ttttcgtact tcttgatcaa tctcgaaagc gacttgttcc
     4141 gagtaagctt ttgtttgacc atagtcgcgg ccaacaaaca cttgatggtt tccttcatat
     4201 tgaacaggac ctaaacgatc actcattcca tattctgtta ccatgcttcg tgctagagct
     4261 gtcgcttgct caaagtcatt ggaagcacca gttgattgga cattgaagat gatttcttca
     4321 gctgtacgtc caccaagtaa gcctacgatt tgttcaaaca tatcttcttt cgtcatcaag
     4381 aactgatctt cttttggtaa agcgatcata tatccgccgg cgcgtccgcg agggatgatc
     4441 gttactttgt ggacgacccg agcgcgactc aatactaatc cgacaatcgt atgaccagct
     4501 tcgtgataag caaccatttc gcgttctttt ttgctgatta cgcgatcttt tttagctgga
     4561 cctgcaatca cgcgatcttc cgcttcatcg atatcagaag catcgatttt ctttttattt
     4621 cttctagcag ctactaacgc tgcttcattc aagacatttt ctaaatctgc tccggcaaaa
     4681 cctggtgtct gttgagccac aactttcaag tcaacgtcat ctgccatcgg tttattccgt
     4741 gcatggactc gcaagattgc ttcacgacct tttacatctg gacgtccaac taaaatctgg
     4801 cggtcaaaac ggcctggacg tagtaacgct gggtctagta catcagaacg gttggttgca
     4861 gcaatgacga ttactccttc atttccgtcg aagccatcca tttctacaag caattggttc
     4921 aatgtttgtt cgcgttcatc atgtccgccg cccataccgg caccacgttg acgacctacc
     4981 gcatcaattt catcaatgaa aatgatagct ggtgcgtttt tcttcgctgt ttcaaacaaa
     5041 tcacgaacac gacttgctcc aacaccgaca aacatttcta cgaaatcaga acccgagatc
     5101 gagtaaaacg gtactccagc ttctccggca acagcttttg ctaataaggt tttacctgtt
     5161 cctggaggtc cttctaaaag tacacctgcc gggatacggg cacctaattc aacaaatcgg
     5221 cgaggatctt tcagaaattc aacaacttca actagttctt gtttttcttc ttccgcaccc
     5281 gctacatcag aaaaacgtac gcgattagct tttttgtctg cttctttggc ttttgattta
     5341 ccaaagttca tgacacggcc gccaccgcca ccgccgccgc cttgttgtcc catcatcatg
     5401 taaaagaaaa agatgatgat tacgattggc aagaagctaa gcaataggga aacccatata
     5461 ccgctgcttg attcttcttt tactgttgta gcaactttgt tttcttttgc cagttctgtt
     5521 acttggctca atgttgtatc atttggcaaa atgattgttg tgaaatgggt agtagtcgta
     5581 tctgttgaac ctaagatagc taacccaccg ctgttagcga cggtctgctg ttctttgtat
     5641 tcaccagtga tcttatatac accatttgca ggctgcactt caaattcttt gatcttgcct
     5701 gcttgtagtt gttctgtgaa ttttgagtac tcgatatctg acgatcgctg gccgttatta
     5761 ccaaaaatga aataaacgac cattaccatc gctaagatta cgaggacata atacaaggca
     5821 tttttcatca tgccgttgtt ctttttgttc atagttgccc tccctaagcg tcaatttaat
     5881 ttaacagttt tttatcaatc ctgaccattc tattgtacca taatttaatt gagatacagc
     5941 attaatttga ttgataaaca ctaggtttca atacaccgat gtacggaagg ttacgatatg
     6001 cttctgcata atccaagcca taaccgacca caaattcatt tggtacgttg aagccaacat
     6061 aatctgcttc gatatttact actcttcctt caggtttgtc aagtagtgta acaattttga
     6121 ctgattttgc tttgcggtat ttgaaaagat ccactaaata agcaagtgtt cttccgctgt
     6181 caatgatatc ttcaacgatc aacagatcgc gaccttctac attcgtatct aagtctttga
     6241 cgatttttac ttcccctgac gagactgttg cattcccgta gcttgaaaca tccatgaaat
     6301 ccaattccaa atacgtatcg atagaacgaa cgatatctgc catgaatggt accgctcctt
     6361 ttaagacacc tactactagg ggattggtgt tttggtaaat ttctgttaac tctttgccta
     6421 gttctttgca acgaacttgt atctcttctt gtgagatcaa aattctctca atatcctttt
     6481 ccaacatcag gttctccttc caattgcttc tttttgatat ttgtaaagaa gtatgtagtg
     6541 tattttatca gtttcttcac gaatactcaa atatgaaaag gcaaaaggta tcaagctata
     6601 tatatttctt ttggcatctg taatgatcca agcatgctgc ctttgtgaat taggtacttt
     6661 ctgatcgata aaatatctag atactttttt tgtgagattc tctgttaatt taatgcggtc
     6721 tcctgctttt cttttttcga catacagttg cacatcagca ggaatccaaa tgtcatgaga
     6781 aaatagagac catgaatctg ttgctttgat gtcccctgca ttttcaggtt tgaccagacc
     6841 aatccattct gtttcattca aatatattcc ttgttctgga atcagcaaca agcgctggtc
     6901 ttctgtctgg ctagttgctt gtttttcttt tttagagagt acaacaaaat gatactctct
     6961 tttcagtatc caattattgc caatactgag ttgccattgg ctttttttct gttgccactg
     7021 gtctaaaatc gactccattt gttgttcttt caaaataatt cctgtttttg agaatgcttt
     7081 gtcgaaaaag gagtaaaaaa aatagtatcg ttcagcatct gtcatttctt caatcatttc
     7141 tgcagaaaat cgaaattgtt cctcccactg ttcgacttcc ttttcgatca actgtcccat
     7201 atatttttga atgacttggt ctgcccattg gatctgttgc gaaaattgct ggaaatgtct
     7261 caacgcctgt gtattttctt gtttcaattg aggaactacc aaatgacgaa gccgatttct
     7321 ctggatatct aacaattggt ttgtctgatc ctcaaagtaa acaagctgcg attccgctgc
     7381 ataagtatat aggtcttctt tgctaaaact cagcaacgga cgaatcagtc tgcccgtagc
     7441 aaatggttgt acttctttga ttccagaaaa agtatttaac tgtcccccac gaacgatttt
     7501 cattaacacc gtctcgattt gatcatctcc gtgatgagca gtcatcaacg tgtcatattg
     7561 tccttcttcc atcacgcgag tgaattcttg ataacgaaag ttacgagctt ctgtttctat
     7621 ggcggatttg gctggatctt cccaaatact tacatataaa gggagattct tctcttcaca
     7681 atatgagcga agataggcag cttcttctgc tgattcttct cttaattggt gattgatgtg
     7741 gacgatacct aaagaaaaac cgatttggcg ttgcacttgt aaaagtaaat ggagcaaaac
     7801 catcgaatca acaccacctg ataccgctgc taaaattctc gatccttgtt gccagaatcc
     7861 ttgttcattc ccgtgccgaa taaatgactg ctgtaatagc ggtgcttttt tcccgccttt
     7921 ttcaacgttc atcctttcca caccctttct tcctaaaaaa aagagagcgg gaaaacgtct
     7981 aaaacgacaa ttaacctgcc ctctgttgat actatgaccc aaatagtcgc actatcatta
     8041 tttgcggctt tttagctgcg acgtccgcca cggccaccgc gctttccttc tgtgtttcgc
     8101 ttcaatgaag ataagcggtc atcgctatct ttcaaaaatg aactcatcaa tgaatcgaag
     8161 tcttcttttt gagctgaagg ttgactgcga gaaaaagatt ttttctgtgc tggacgattt
     8221 cggttgtcac ggggatcttt ttgttgatat tttttaggac ggctttcttc tccagattca
     8281 gcaggttgtt cctgcgcttt ccggatagat agtccgattt ttccatcatc atttactgaa
     8341 ataactttaa ctgttacttc atctcctaca cttaaaacat catggatgtc ttttacatag
     8401 ccatttgaca cttcgctaat atgaactaga cccgtttttc cagcacctag atcaataaac
     8461 gcaccaaaat ttgtgattcc tgatactttt ccttgcagtt ttgctcctac ctcgattgac
     8521 ataaaaaaaa tgttcctcct aattatttcc cttttttata ttcctgctta ttttgcagtt
     8581 gtagaactag ttgattgatc tgtttcattt gtcgagctgt ctgtatttga ttctaatata
     8641 ggataaactt tttcaccgtc ttttgagtaa aagaatttgc ttctggcaag tttagctaca
     8701 tactcatcat ctttcagcaa tgaaacatct tggtgcagct gatcaacgtc tgcgttgact
     8761 tctttcattt cagtatttgc ttcgactctc aattcgttca attgatgcat cctttgcatg
     8821 tcatggaaaa tctgcacacc tattatcgcg aaaatgacca aagcaccaac aaaaattgca
     8881 gccagtctgc gtcttcgaaa aatcagttga cgttgttgtt gctgaaattc agcatacttt
     8941 ttctttgcat attccgtatc tagcgcagcg attttattgc tttttttcgt cacttggatt
     9001 cgctccaatc tttttgattg tctttttcat tataccaata tcgtgaaagc ttgtctaatc
     9061 tatttctcta gtgtttctgt gcgattttca gaaataattt cgtacatttt ttgtgcatct
     9121 tcttttttgg ttgattcatg taattccgta attttcactt ccaacgtttt gttgccaaac
     9181 tggactttca aaatatcgcc tactttgaca tcactggaag atttcgccaa tttaccgttg
     9241 atttgaattc ttcctttatc agctacttct tttgctactg atcgtctttt gatgattcgg
     9301 gaaattttta aaaatttatc taatcgcata tcttattcct cactttttaa tttttttatt
     9361 taatcgcagg attttcttac caaaaggcaa cattagccat tctcgtactg taaacagacg
     9421 aagacgaatc actgcataaa tgaacacaga agccccgatc ccaactccta tcacgctgac
     9481 aaaaaaagca gtggaacgat gagttacagg accaaatact atattcaata caccataata
     9541 cagcagcagt actactgtca tacaaagaag gctgaacatc aacttccgtc caaaatgccg
     9601 ttctttccaa aaagcattta gctcttttgt ttcaattcga acaaaatacc aaagcgtcac
     9661 acctaatcct aaaagggtag acaggcttgc acctgctgtt cctaataaac cggtcagaaa
     9721 agaagtagtc aatcctttta ccaatagtcc ccatcctgcg ccttttaaag aagggcgaaa
     9781 cgagttcttg ctttgagcaa tactttgata agcttggatg actgcagtga aagcaatgga
     9841 aaaaacaaat aatacaagcg ctgcatttcc cgcataatct ttaaacaacg cataattgat
     9901 ataagggagc agcaaggcta atcccaaaga cgcagctaat gctaaagcgg tcgtcaatct
     9961 aagatagatt ttggctgtct gtaaaaactg acgatagatc cggttggtca aatgacgagt
    10021 caacgcaggc aggaatgtcg cacttaatgc agtggcaatc actaaaccta attgtactaa
    10081 aggctgacct cgatcataga ccccttttgc tatcttcgca ccgtattctg acagtccgta
    10141 aacttccaaa gcatttttta caaagaaaga gtcgatcagc tggaaaaaga taagcaatcc
    10201 actataaatc gaaaccagtc cgccttctac cagaaaacgc cggatcaagg gctttgacgg
    10261 ctgtttgaac aatggaaacc gtcggaagct taacgctcct ccatgaatct tttgttcata
    10321 gtagtgaagg atcccatatg cgcataaacc gccaaaaaca gctccactca ttgctgccgt
    10381 ccctgtttgg tacaccgtcc atccaagctt tctaaaagca aacgcactac agacaatcac
    10441 aaaaacacga acgaactgct caactacttg agaaacagca gttggttcca tcaagaatct
    10501 tccttggaaa ttccctcgat aaaaagatag accaggcatc agcaaaaagg taaaggaaac
    10561 tacttggatc agcggtttca attgtatgtc tcccatcatc caagcaatcc agctgctaaa
    10621 tgcaaagaca aacaaccaca aaaagacacc tgtccaaaat actaagggat aaagattttg
    10681 caaagcttgc tttcgctcga aaggattctt tctttctgca acgtactttg atatgaactg
    10741 cggaagaccg gataaagcta aagtcattgc tatcccataa attggataaa cttgctgata
    10801 aacataaaaa ccttcgtcgc cgactaagtt ttggagggga attcggtaaa gggcacttaa
    10861 tacttttgct atgaaagaag caatcgtcaa tacaaatgct ccttgcatga cgcgccgcat
    10921 ttctttatga tccaaatcat tgctccttcc tacgctgaca gatatttttg ctcacgcaac
    10981 gctttgacaa acttttggat ttcctgaagc catactgctt cttccatttg gttggggatc
    11041 gtcaagcgta tcaccatctg ttctttttca acgcctagac tggcttttag ttttgtcgct
    11101 gtcagtgctt cgaataactg ttccacacta tagcgttttg ttccaacttt acttagagta
    11161 aagactatat gcgcctcctg tttccttatc gtttctagga gcgctcgatc gccatccatt
    11221 ttgatctgcc caattgtcaa gagatgtgct acttcttctg gatattctcc aaaacggtcg
    11281 agcagatcat cttctagttc atctagcatt tcttgcgaat caagttcacg aattcgttta
    11341 tagatctcga ttttctgccg ttgatctgcc acgtatgttt caggtagata cgcatcgaca
    11401 ccgaggtcga tctcaacagt ggtcttctcc acttgactat tctttccttg tttacgagaa
    11461 acagcttcgc ttagcatttg agaatacata tcgaaaccga ctgcatcaat aaatccatgc
    11521 tgctgagcac ctaagagatt acctgcaccg cgaatggaaa gatcccgcat cgcaatcttg
    11581 aatccagatc ccagttctgt aaaatctttg attgcctgaa gacgtttttc actgacttcg
    11641 ttaaggatct tctgctgctc atacataaag taagcatatg cgacacggct gcttcttcct
    11701 acccgccctc gcaattggta taacgtggat aatcccatat aatcagcgtt ctcaacgaac
    11761 aacgtatttg cgttcgggat atctactcct gtttcaatga tggttgtagt gacaagaaca
    11821 tcatattgac cttcaataaa ttcaaaaagc gtattttcta attgcgcctc tgtcatttgc
    11881 ccatgagcat aaccgatccg tgcttccgga acgagttctt ggatttcttc tactttttgt
    11941 tcgatcgttt ctacccgatt atataaatag aatacttgtc cacctcgtcc catttctcga
    12001 tggattgctt cgcgaatagc cccaggattt ttttccataa catacgtttg gatcggataa
    12061 cgattcgcag gcggtgtttc gattactgaa agatcccgca cgcctaacat cgacatatgc
    12121 aaagttctcg gaatcggtgt tgcagtcaga gtcaaaacat ccacttgtgc acgtaactgt
    12181 ttcaatcgtt ccttatgttt tacaccaaaa cgttgttctt cgtcaatgat cagtagaccc
    12241 aaatcgctaa attcaatatc ttttgacaaa attcggtggg ttcctacaac aatatcaact
    12301 tgacctgtac gcagttgttc gatggtttct ttttgttgct ttcttgttct gaaacgactt
    12361 aatagcccga tatttacggg gaaaccttca aatctttcca gcatcgtttc atagtgctgc
    12421 tgtgccaaga tcgttgttgg aactaaaaag gctacttgtt tgctttcctt tatcgcttta
    12481 aaggccgctc gaagcgctac ttctgttttc ccataaccaa catcacctac gagtagtcga
    12541 tccattggtt tttctttttc catatcccgt ttgatctccg ctgcacttct caactggtca
    12601 tcagtttcag agtaggggaa ggcattttcg aattcttttt ggtaggcgtc atcaggtcca
    12661 aaagcatatc ctttttctga ctctcttgcg gcatataatt tgattagatc gtcagcgata
    12721 tcctcgattt ttgaagagac ttttcgcttg gttttcgtcc attcacttcc gcctagctta
    12781 ttgatgcgag gcgctttggc ttcagaggcg acatattttt ggatcagatt cagttgagta
    12841 acaggaataa acagtttgtc gtcgttttga tacaaaatag tcatatagtc ttggtgaacg
    12901 ccatctactt ctagcgtttc catcccaatg tatttcccga taccatgatt ggcatggaca
    12961 acgtaatctc ctgcttttaa ctcattatag cttttcaatc gctctgcgtt tgttactgtt
    13021 tgacgtctcg tttgtttttt cgtcgttttt tggaaaattt ctttttctgt gatagctact
    13081 attttttctt gaggaagttc aaaaccagcc tgcaaagctc cttcaactaa ttgtacctgt
    13141 cctctgatca atgcattttt ttctgtttct actactaata tgtcttcatc tcgaagcatt
    13201 tgttctgctt tgcgtattct gtcctttgtc ggaataaaga tcacaacagt ctgtttttgc
    13261 ttgcgccaac ggtccatttc tgttttcagc aatggcattt gtccgaagaa ctgttgcatc
    13321 gtccgatatt gaaaattatg gattgcttgg aaacgaagat ttcccatccc tttttggaag
    13381 agtgagaaaa aggttgtggt gaacttttcc ttctgcacga ttttttgtat atctgctgaa
    13441 aatgtctgct cagaaaacac ccgcaattcg ctgatttttt gagtctgcca ttctgccgct
    13501 tccctttcga tttcccgatt tgtttccatg attcgctgat aatcatcaac gactagtaaa
    13561 ctatttgaag ggaaataatc caagatcgtc acagattctt ggtaaagaag atctgagtag
    13621 aattttgctt cttctgtcgg gacacctgct tgccagcttg aagtcagctg accgaagtaa
    13681 tcttgcaaaa actcgcggtc tgcttcttct gtggtcgcag caagccgttt ttctagcatt
    13741 ttttgcattt gctctattcc atgttctaga tcctctggtc cgtagacttg ttcactcgtt
    13801 ggggaaaccc agatttcttc cgttttttcg attgagcgtt gtgtttctgc ttcaaaatac
    13861 cgcatagaat ctatctctac atcaaatagt tctacacgaa caggataggt tgtcgttaac
    13921 gggtaaatat cgataatgct tcctcgaata ctgaattctc ccggttttgc gaccatctct
    13981 tctcgtacat agcccatcaa agtcaattgg cgaggaagat tttccacgtc tatctcatca
    14041 ccaatatgcc aattcaattg cgcctgtagc caagttttct tattcggcag tcgtttgtgc
    14101 aaagatgcga ctggcaacaa atagatacct gctttttttt cttgaagtgc gttaagcgtt
    14161 gcaactctct cgcctcgcgc ttctggagaa gaaaaggcca tttcagcaga aagcacttca
    14221 tccactggaa ataaatggat ctgatcttgc ggaatcacat gttgcagatc ttctgctaac
    14281 tgattcgcat aatacaaatt tggcatcaga ataatcatgt ttttatttgt tttcttaaac
    14341 gctcgagcaa atagaagtgt tttcgctgag cccgctaatc ctgtgattag ttgtctaggc
    14401 attttttctt ctaaattgct gacccattct tttacttgcg gatcttttcc gactaattga
    14461 ataatatcca ttccttttcc tccttcctca attgaatcga ttcatcgcat ccacgaacga
    14521 atgcccttct atgagatatt cgactgcttt aacagattga cacatgcttt cttctatcaa
    14581 aggctgattt tctttgctga aaggactaag cacatgctgg actactgttt ttttcccttc
    14641 tggcctgccg attcccacct tgattcgatc aaagacattc gtattcaaat gggaaataat
    14701 acttttgata ccgttatgcc cgccggcact tcccttttga cggagccgga tttttcccac
    14761 tgccagatcc aaatcatcgt agattacgac taattcctct ggataaatcc caaaataagt
    14821 cattagtggg ccgactgcac gaccagattc attcatgaat gtttgtggtt tgactaaaag
    14881 gatcttttct ccattatgaa aaaattcggc aacttctgct tcgaacggat tttttttgaa
    14941 agtagcttga tgctcgcgtg caattttatc cacgaccata aagccgatat tgtgttttgt
    15001 atattggtat ttggtacctg ggtttcctaa cccgacgatc attttcattt ttgttttcca
    15061 cctaattcta aaaagcagca caaaaggctg gactgctaat tagcggtcca gtccatcgag
    15121 cttttttctt ttttatactt gtcaatgcat agtataacac aaaacaaggc gtttcggcta
    15181 aaagagaatc gttaataaat gataaagaaa ttaaaaattt cttcaaagtt taatgaaata
    15241 attgatatat aaaaatagtg aaataacgca catgtaagct tttacatata atacaacctt
    15301 gttatctgtt atattttata gccaaatagc gtgacatggg gcttgagaaa tgttagcata
    15361 agcatgggaa aaaacaaatc aatggaagga ttgatactaa atgactgcag ctgcagaaaa
    15421 aaaagatcat caaaaagtta ttcttgttgg agacggcgcc gtaggttcta gctatgcctt
    15481 tgctctagtt actcaaaata tcgctcaaga agtaggtatt gtggatatca atactgctaa
    15541 aactgaagga gacgcaattg acttgtctca cgctttggca tttacttcac ctaaaaaaat
    15601 ctattctgct acttacgcag acgcacatga tgctgactta gttgttatta cagctggcgc
    15661 accacaaaaa ccaggtgaaa ctcgtttaga cttagtaaac aaaaacctaa aaatcaaccg
    15721 cgatgttgtc acacaaatcg ttgaatcagg atttaacggt atctttttag ttgccgctaa
    15781 cccagtagat attttgactt actcaacttg gaaattctca ggtttcccta aagaacgcgt
    15841 aatcggttca ggtacctctc ttgactcagc tcgtttccgt caagcacttg cggaattagt
    15901 tgacgttgat gcacgtaacg tccatgctta catcttaggt gaacacggag attcagaatt
    15961 cccagtttgg tcacatgcaa atgttgccgg cttgcaaatc tatgaatggg taaaaaacaa
    16021 cccagaaatc gatgaagaag ctatggttaa cctattcttt ggcgtacgtg acgctgctta
    16081 cacaattatc gagaaaaaag gcgctacttt ctatggtatc gcagtagcat tagcacgtat
    16141 cactcgtgcc atcttagatg atgaaaatgc tgttcttcct ttatcagtat tcatgaatgg
    16201 cgaatatgga ttgaacgaca tctacattgg tgcacctgct gttatcaacc gtcaaggtat
    16261 ccaaaaagta atcgaaattc ctttaagtga cagcgagcaa gaccgtatgg ctgcttctgc
    16321 taagcaatta aaagaaattc tagatgaagc ttttgctaaa ctagacgctg aataatcttt
    16381 gaacatgacg gaaaacatct attcggtgtt ttccgttttt ttattttatc acccttatgg
    16441 tttctaaatt ttttcataaa gtagagataa ccttcgtctt ttcctctaaa ttatgctatc
    16501 atgagaccgt tccaccctaa acttttactt aaggagttgt cgatatgaca agatcttcgc
    16561 atcgaaaaaa atcgaaacat gttgctttgc tttcagttct tggtgttctc ttagtgctga
    16621 tcagctttta tacctatcgc agtacatact acacaaagca ttttttgccg aatacagaaa
    16681 taaatggaat aaatgtcagc aatctaacag tcgaaaaagc aaatgaaaaa ttaaaagaag
    16741 cttattctga taaaaaactg gctattaaag aagatggaaa agtctggcag gaagtaccaa
    16801 aatccgagct cggctacaaa gatgatttca cttcggagct ttctccgatc ctaaatgaac
    16861 aaaacggatg gacttgggga atgacttatg tctcagcggc agaaaaacaa gaaatagatc
    16921 ctgtcagtca aaaccaccaa aaactagaca ctaccgttca gacattgaca actaagctga
    16981 ctgatttgaa caaagaccgg acacctacta ctgatgcaac gatcgaaaaa gcaggtgact
    17041 ctttcgttat caagcctgaa gtcaatggaa atacgatcga cgtcgatgct gcggtcaaac
    17101 aattaaaaag tgcggtaaac tctggtaaag atacaattga attgactgaa ttcaaagaaa
    17161 aaccaaaagt aacatctgaa gacagctcac tgaaagaaca actcgcatcc atgaatgcta
    17221 tcgcaaatgt aaaagctact tacagtataa acggtgagac tttccagatc ccatcaagtg
    17281 atattatgag ttggctgacc tacaatgatg gaaaagtcga tttagataca gaacaagtac
    17341 gtcaatatgt aacagattta ggcacaaagt ataacacaag tacgaatgac acaaaattta
    17401 aaagtacaaa aagaggagaa gttacggtcc cagttggcac ttacagttgg acgatccaaa
    17461 cagacagtga gacagaagca ttgaaaaaag cgattctggc aggccaagat tttactcgtt
    17521 cacccattgt gcaaggtggc acaaccgctg atcatccatt gatcgaagat acgtatatcg
    17581 aagttgatct ggagaaccag catatgtggt attacaaaga cggaaaagtg gcccttgaaa
    17641 cagatatcgt ttccggaaag ccaactacac ctacacctgc tggagtcttc tatgtttgga
    17701 ataaagaaga agatgcaact ttaaaaggaa caaatgacga tggaacacct tacgagagtc
    17761 cggtaaacta ctggatgccg attgactgga caggtgttgg tatccacgat tcagactggc
    17821 aacctgaata cggtggagac ttatggaaaa cccgcggttc acacggctgc atcaacaccc
    17881 caccaagtgt aatgaaggaa ttattcggga tggtcgaaaa aggaacgcct gtattggtct
    17941 tctaacaaaa ataatatata aaaagaaagc acccgtacga ttggtacagg tgctttcttt
    18001 ttattatcta acaatgtgct acttacattt cagcagcagt aaagacttct gaaacatcat
    18061 catcgtcttc tagcttatct actagacgtt ccaattgctc tttttgttcc tcatttaaat
    18121 caatcgtcgt ttgtggaatc atcgtcaatt ctgcttgcgc caaagtgaac tctttttcca
    18181 actcatctcg tacagcagca aaatcttctg gtgcagtata aatctcgaat acttcaggag
    18241 aagtttgcag atcttctgcc cccgcctcta atacatcctc aaacatcgtc tcttcatcaa
    18301 cagtcaaccc atcgcgttca atagcgatat atcctttgcg gtcaaacaag taactgactg
    18361 aacccgtttc tcccagtgat ccaccgtttc gagtaaaagc tactcggaca tttgtcgctg
    18421 tccgattacg gttatctgtc aatgcatgga ctaagattcc tactccaccc ggaccatatc
    18481 cttcatacgt gatttcatcg taattcgttt catctacggc actcgtagcc tttttaatcg
    18541 cccgttccac attgtcgtta ggcatatttg cagcttttgc tttgtccaca acaagccgca
    18601 aagctggatt catagatgga tcaggaccgc ctgctttagc tgccatataa atttcacgag
    18661 aaagtttctg aaagactttc cctcgttttg catcttgggc attttttctt ccttggatat
    18721 tatgccattt actatgtcct gacatgattt ttcctccctt gttcttttga gaatacactt
    18781 ctattctagt actatttcac aaaaaaacaa agccacagcc aatctataaa cgcttcttga
    18841 atgccgcaaa aagcacacat aatatcacag cagtcagagc accgatcgaa tccaacatga
    18901 catcttggaa cataggtgtt cgtccaccgg tcatcatctg atggaattcg tccaaacctg
    18961 cgtaccctgt tgctgaaagc caagcaagga tcgctgtcag ccaccaaatt tttagcttgg
    19021 ggaaaacacc taaaaacaag ctgcctccca aaataaaata ggtaccgaaa tgtgcgccct
    19081 tccttaaaaa gaactctaaa aatttggcgt agcccagatg gtcgatactc accatacttc
    19141 caccataatt aaaacgtata cccgctaatg tgtctttaaa tggctgatta ggcagccagc
    19201 tttctaatcg cgaaacttgt gattgctgtc cgtaagtttg agaagaactg taaaataaga
    19261 ttataacgac tatcagtgcg attactaaat aaatatttcc gtttttcatt tgtttttttg
    19321 aagaattcat aagtcacctc tgtattttat cttagacttt ttttgaagaa acaacaatca
    19381 ttttcataga gacttcttcc ataaaaaagt gctatactta attttgtatt acaatatgaa
    19441 gggagttttt tcatgagtta ttcattaaat tggaatctag attctatttt ttccggaggc
    19501 agtcattctg atgctttgaa ccaacggatg aagcaattgg aagaccaaat gaacgagtat
    19561 tatcaccgcg taacaaaatg gtcacctagt tctgacaatg cagagcagtt aaacgctatt
    19621 cttcaattgc aagaaacgat tacgaatggt tttacccaat gcagcagcta tattactgca
    19681 ttgctttctg ccaatgtcaa tgattcagat gcaaaggtct tatcaggcaa gctttacgca
    19741 atgctgcctc gtctgcaatc agccgaaact gtattgtcaa agaaatttgc agaaatatct
    19801 gacaatgatt ggaatcagat gctttctcaa tcttcgtttg aaacaattgc ttttcgttta
    19861 aacgagattc gtcgtgatgg ctcacaatta ttatcagaag ctgaagaaaa cattataaat
    19921 acgctttctt tagatggtct aaatgcttgg agcagtcatt atgatacgat tgtagccagc
    19981 atttcgattc ccttcgaaca agatggacaa gtagtcgaat tatctgctgg acaagccttc
    20041 aataagatga tgggcgatcc agatccaaaa gtcagagaaa cacttttcgc agcatgggaa
    20101 gaagcctgga aagaaaaggc acccctcttc acggatactt tgaatcattt agatggtttt
    20161 agactttctg actatgaact tcatggtgtc acagattttc tacaaaaacc attagagtac
    20221 aatcggttga agaaagaaac attgtctgtc atgtgggata caatccagaa aaacaaacaa
    20281 ccccttgtta attacttgac tagaaaagct aatcttttcg gtaaagaaaa aatggaatgg
    20341 caagatcaag atgcacctat tattttaggc gatttgaaag aaaaaacctt tagttttgac
    20401 gaagcagcag catttattat cgaaaacttt aacaaattca gtccaaaaat ggctaaattt
    20461 gcccaatctg cttttgaaaa gagctggatc gaagcagaag atcgtcctgg gaaacgtcct
    20521 ggtggttatt gtactgagtt accagaaaca aaagaatcaa ggatctttat gacctacagt
    20581 aattctgtta atgaggttgc gacacttgct catgaactag gacatgcttt ccatagcagc
    20641 acgatgtggg atttaccttc tttaaatcgc gaatatgcca tgaatgtcgc agaaacagca
    20701 agtacattcg cagaactgat cgtagccgat gctacattaa aagtcgcaaa aacaaaagaa
    20761 gaaaaaatca atttgctaga tacaaaactg caaaatgctt tagctatgtt catgaatatc
    20821 cattcccgat tcatttttga aaatcgattc tatgaagcac ggcaagaagg attagtctca
    20881 gaagataaga tcacagagat gatggttgag gcgcaaaaag aaggctatca aggagcttta
    20941 gctacttacc atccatattt ctgggctgca aaattgcatt tctttattga tgacgtaccg
    21001 ttttataact tcccatacac atttggctac ctctttagtt tgggcatcta tgcttacgcc
    21061 aaccaaaagg gagcaaattt tgaagaagaa tatatccatt tactacgcga tactgcctca
    21121 atgacgacgg aagatcttgc gaagaaacat ttgaatgtcg atcttaccca accagatttt
    21181 tggcaagcag gtatcgacat ggtgactgaa gatatccaaa cgttcatgga attgacagaa
    21241 gaatttatct gatttctatt ttatcacaat aaaaaaagag ttagtccaga atgggctaac
    21301 tctttttgat taatcattct tacatgctga cagcaaagtc aggggcaaac cattgaactg
    21361 aaccaacttt tacgctttcg cctggttgag gagcatggat atattgtcct ccgcctaaag
    21421 caatcgctac gtggtaagtt ccgccttgtg acccccagaa taacaagtct ccagcttttg
    21481 cttgagaaac tgaaattttt gttccagctg attcttgagg aactgtccaa ccaccgatgt
    21541 cacgaccagt tacttgcatg taaacatagc gtgtgaaccc tgagcagtca aagccacttg
    21601 gatctttacc gccccaaaca tatggagtac caatatattt gtaagcttct gctacgattg
    21661 ctgcaccatt cacacttcca gaaggagctg gagcaggtgt tacaggtttt ggttgttcag
    21721 gtgttggtgt tggcgttgga gccggagtac tacttcctgt gccgtttcct gtatcaacac
    21781 tttgatctgt tgatggtgtt gtaggcgcag gtgttgttga ttcttctgtt gttgaagatt
    21841 caggcactgt tgttgattct tctgttgctg aagattcagg cactgttgtt gattcttctg
    21901 ttgctgaaga ttcaggcgct gttgttgatt cttctgttgc tgaagattca ggcgctgttg
    21961 attcttctgt tgaagattca ggtgttgttg attcttctgt tgctgaagat tgtgtagctg
    22021 atgatgattc ttcagtcatt gaagattgtg tagctgatga ttcttctgtt gctgagcttt
    22081 gtgcagtaga tgaagcttga gtagcttgtg cttcagcctc tgcttgttcg cgtgcttctt
    22141 tttcagcttt ttcttgtgca gcttgttgac gagcttgttc tgctagacgt tgttgttcac
    22201 gaatacgagc ttgctctgct tcagcttctg ctttttgacg gttcaagtca gctttcttgt
    22261 cttcagcagt tgcttgttca gcagctaatg aagttttcag tacgttcaaa tcagcttgtt
    22321 tagataaaag atcgcctttt tgagattcca aagcagcttg gttttcagct aattctttta
    22381 gtttagcatc gttttcagct tttttgtctt ctacagcttt tttatcttgt ttttgttgtt
    22441 ccatcaagtc gttgtttgct tttaccatcg ttgtcattgc ttgaacgcgt ccaatagcat
    22501 ctgctaatga atcagcattc aatacagcat cgatgtagtt tgagcttgtg ttgcttactt
    22561 gtgcttcgcg tgcttgtttt tggattgtat cttcacgttt ttcaatacgt tcttgtaaat
    22621 ctgcaatgtc ttttactaat tgtgcagatt cttgacgcaa tgtatcttgt tttgctaata
    22681 ggtcttgtgc ttgtgtgttg atttcagaaa cttggctttc taaggcatcg atttgtgatt
    22741 gtgcatctgc ttgttgattt ttcaagtctg caatcttttg atcttgctgt tgtatctgag
    22801 aatcaaaatc gtccgccgcg gcagcgatcg gtgatgctac ggcagtcaag gtcattgaac
    22861 ataccattac tgctgatatt aaactctttt tcactcttca ttcctccgac tggctt
//
LOCUS       DAKBVG010000016        22891 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000016 DAKBVG010000000
VERSION     DAKBVG010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 22891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.016"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            151..519
                     /locus_tag="OUD09_000510"
     CDS             151..519
                     /locus_tag="OUD09_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HCT9691809.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            699..1640
                     /locus_tag="OUD09_000511"
     CDS             699..1640
                     /locus_tag="OUD09_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCT9691810.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1709..2440
                     /locus_tag="OUD09_000512"
     CDS             1709..2440
                     /locus_tag="OUD09_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HCT9691811.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2584..4764
                     /locus_tag="OUD09_000513"
     CDS             2584..4764
                     /locus_tag="OUD09_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCT9691812.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4784..5266
                     /locus_tag="OUD09_000514"
     CDS             4784..5266
                     /locus_tag="OUD09_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HCT9691813.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5282..6043
                     /locus_tag="OUD09_000515"
     CDS             5282..6043
                     /locus_tag="OUD09_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCT9691814.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6174..7508
                     /locus_tag="OUD09_000516"
     CDS             6174..7508
                     /locus_tag="OUD09_000516"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCT9691815.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7815..8300
                     /locus_tag="OUD09_000517"
     CDS             7815..8300
                     /locus_tag="OUD09_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HCT9691816.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8293..8976
                     /locus_tag="OUD09_000518"
     CDS             8293..8976
                     /locus_tag="OUD09_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCT9691817.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            9102..11747
                     /gene="polA"
                     /locus_tag="OUD09_000519"
     CDS             9102..11747
                     /gene="polA"
                     /locus_tag="OUD09_000519"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCT9691818.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11792..12628
                     /gene="mutM"
                     /locus_tag="OUD09_000520"
     CDS             11792..12628
                     /gene="mutM"
                     /locus_tag="OUD09_000520"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HCT9691819.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12592..13221
                     /locus_tag="OUD09_000521"
     CDS             12592..13221
                     /locus_tag="OUD09_000521"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCT9691820.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            13273..13605
                     /locus_tag="OUD09_000522"
     CDS             13273..13605
                     /locus_tag="OUD09_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCT9691821.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13917..14408
                     /gene="nrdR"
                     /locus_tag="OUD09_000523"
     CDS             13917..14408
                     /gene="nrdR"
                     /locus_tag="OUD09_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HCT9691822.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14431..15804
                     /locus_tag="OUD09_000524"
     CDS             14431..15804
                     /locus_tag="OUD09_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HCT9691823.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15804..16730
                     /gene="dnaI"
                     /locus_tag="OUD09_000525"
     CDS             15804..16730
                     /gene="dnaI"
                     /locus_tag="OUD09_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HCT9691824.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16859..17299
                     /locus_tag="OUD09_000526"
     CDS             16859..17299
                     /locus_tag="OUD09_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCT9691825.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            17443..18144
                     /locus_tag="OUD09_000527"
     CDS             17443..18144
                     /locus_tag="OUD09_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HCT9691826.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            18141..19262
                     /locus_tag="OUD09_000528"
     CDS             18141..19262
                     /locus_tag="OUD09_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HCT9691827.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            19277..20509
                     /gene="pepT"
                     /locus_tag="OUD09_000529"
     CDS             19277..20509
                     /gene="pepT"
                     /locus_tag="OUD09_000529"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCT9691828.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20641..21549
                     /locus_tag="OUD09_000530"
     CDS             20641..21549
                     /locus_tag="OUD09_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HCT9691829.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAIWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21584..21856
                     /locus_tag="OUD09_000531"
     CDS             21584..21856
                     /locus_tag="OUD09_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HCT9691830.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            21867..>22891
                     /locus_tag="OUD09_000532"
     CDS             21867..>22891
                     /locus_tag="OUD09_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HCT9691831.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKK"
BASE COUNT         7718 a         3992 c         5024 g         6157 t
ORIGIN      
        1 aaataacaaa aacaaccaac ttatttatta ttaaagaaaa gcgtatttaa gtaggacgat
       61 tctgttgaga gctttcgtag attttcccat tttcctatgg taaactgaca aagttgagaa
      121 tgttagaaca gatcgtttag gaggaaacag atgtttacga atgaaataaa aatcatgctt
      181 tatgtggacg atgtagaaaa aaatgcaacg ttttggagag cagttggctt tgcggaaaaa
      241 gatcgtcaag aaatggatgg gacgcttata attgaagtag gagttgagga cagtcaaacc
      301 tcatttctgt tatatgatcg agcatttgtt gagtcccatt cacctggcac agctactagt
      361 gccccatctt tgatgtttta cagtaaagat atctttggac tatataaaaa aatgcagacg
      421 caatcagtca cattaggtga tatggtacag cttggagaag aatatgtctt taactttgct
      481 gatcccgatg gcaactactt tgcggttaca ggtaaataat agaaataaag actttgtttt
      541 tctgtgcgaa acaggaaagc gaagtctttt taattcaaat aataatatta tgtaaacaaa
      601 aaactttcca tgcgtaatta tacacaagca aataaatcgt caaatgcgaa cgccttcttt
      661 atactgttta ggaacgaaaa gaaggaagga agaaatacat ggattatcaa gtactgcttt
      721 attacaatta tacaaaaatc gaagaaccag aggaatttgc caaaaatcat ttagcctttt
      781 gcaaatcttt ggatcttaaa ggtcgtattc tagtagctga agaaggaata aatggtactg
      841 tgtctggaac cgttgaaaac acgactgcct atatgaaagc aatgaaagct gatcctcgat
      901 ttgctgatac gatgttcaag atcgatccaa gcgaaggaca tgcctttaag aagatgtttg
      961 taagataccg tccagagctt gtaagtttaa agctagaaga ggacattgat ccaaaccaag
     1021 tgacaggtgc ctatctttca ccgcaagaat ttaaagaagc tattttagat gaacagacag
     1081 tggtgattga tgcacggaat gattatgaat atgacttggg acattttcga ggggctgtac
     1141 gcccagatat acgcagtttt cgagagcttc cagactggat ccgcgagcac aaagaagaat
     1201 ttatggaaaa acgggtagtg acctactgta ctggtggcat tcgttgcgag aagttttctg
     1261 gttggcttgt ccgtgaagga ttcaaagatg tcggacagct gcatggcggt attgctactt
     1321 atggtaagga tcctgaagtc caaggagaat tatgggacgg caagatgtat gtgtttgatg
     1381 aacggatcgc tgtcgatatc aaccaagtag ataaacaaat cgtcggtcgc gactggtttg
     1441 acggaacacc atgtgaacga tatatcaatt gtagcaatcc tgagtgcaat cgtcagattc
     1501 ttgcttccat tgaaaatgaa gaaaaacatt taggagcatg tagcatagag tgtgcgaaac
     1561 atccgcgcaa tcgctatgtt caagaccatg gaatctctcc agaagaagca gaactgcggt
     1621 ttacagcttt aattaaatag aaaacaatag ttttgattgt cgaccatttc caatatgcgc
     1681 tatactagcg ttgaggatgg tgatccacat gaaatcattg atttatgcta ttggggatgt
     1741 ccatggcaaa catgagcttt ttgaaaaact tattcactat tatgatccaa agatccatca
     1801 gcttgtttta ttaggggatc tcaatgatcg aggtcctcgt acaaaagaat gttttttatt
     1861 aggaaagcaa ttagtcgaag aaacaggagc catttacctc cggggaaacc atgaagaata
     1921 ttttttgcaa tttctgcaat caccggagga ctggtatgcg ccttatatga gaaatggtgg
     1981 aaaagaaacg tttgaaagcc tgattcaccc aggggcatcg gaggaatact ccccaactga
     2041 aatctcgatg atgatccaat cgagatattc agaactgatt aaatttttag tccagcgccc
     2101 cttgtattat gaatgggaaa attatctttt tgtccatgcc ggtgtggatt tgacaaagaa
     2161 agactggcgt aagacaagtc taagagactt tatttggata agggaaccat ttcatgaagg
     2221 gaaaaacaat acaggtaaga caattgtatt tggacatacg attacaccaa tgctccatgg
     2281 agatatgcag acaacagacc tctggattca ggatcataaa atcggaatgg acggtggggc
     2341 tgtattcgga gggtcattgc atggggttgt ttttgacaag gaaggtattc ttcaagatat
     2401 tgaatatcag aacaacgaag gaccttggca tggaatctga ctaatggaaa gaatgaagga
     2461 gctgtgactt tgttgcggct ctttttattt ttatagagaa tattgtttct ttcagtagtt
     2521 taacccagga taaaaagtat gatagaataa agaagcaatt tttagcagaa aagaggcatt
     2581 ttaatgacgg aaaatttgaa ccagacgatc attcagcttg tacaaaaaga actttcagac
     2641 tatcgtccta agcagttgac tactgtactt aatttgttaa atgaaggaaa cactgttcct
     2701 tttatcgcgc gttaccgaaa agaaatgact ggaagtcttg atgaagtcca gattcgtgaa
     2761 atcgaagagc ggtatgccta tcttgaaaac ttagaaaaac gtaaaactga agtgattcgg
     2821 ctaatcgatg aacaagggaa actgactccc gagttagaag cagaaatcac ccaagccgtg
     2881 aaaatgcaac aagtggaaga tttgtatcgt ccatacaaac aaaaacgacg gacaaaagca
     2941 acgattgcta aagaaaaagg acttgaacca ctcgcgaagt ggctgatgca gcttacagat
     3001 ggagaggtgc aatctgaagc agaaaaatat attgacaaag aaaaagaagt ctcttctgcc
     3061 gaagaagcgt tgcacggtgc tcatgagatt attgctgaac aagtcagcga taatgcaaaa
     3121 tttcggactt ggattcggtc ttatacgtat aacaaaggta tgtatgtcag caatgtcaag
     3181 gatgagcaag cagatgaaaa aggcgtatat gaaatgtatt atgactttgc agaacccgtc
     3241 cataaaatgg tttctcatcg aatattagct actaatcgtg gagaaaaaga agatattctg
     3301 aaagtattct ttcaagtaga tgaagcagca attttagctt acttggatcg ccaaatagtc
     3361 aaaaatcctg cctctccttc ttcttcattc gtcagagaag cctatcagga tagttataaa
     3421 cgctttattc agccagcaat cgagcgagaa ctgcgcaatg aattgactga aaaggcagat
     3481 gagcaagcaa tcgctatttt cggcgaaaac ctccgtaatc ttttgttgca accgccacta
     3541 aaaggtaaag ttgtcctggg atttgatccg gcttatcgta ctggatgtaa actagcagtc
     3601 gtagatgcga cagggaaagt tttagcaatc gaagttattt atcctcataa acctgcagcg
     3661 caagcaaaaa gagaagctgc tggaccagct tttatccaat taattaataa atatcaagta
     3721 gatatggtag ctatcggaaa tggtacagcc agtcgggaat ctgaactttt tgtggctgag
     3781 cagttgaaat ccgccgacca taaaacctat tacgctatcg tcaacgaagc aggcgcttct
     3841 gtttactccg ctagcgaaat tgcacgaaaa gaatttccgc atctacaagt tgaagaaaga
     3901 agtgctgttt ctattgcccg tcgcctgcaa gatcctttag ctgaattagt caaaatcgat
     3961 cctaaagctg tcggagtagg ccagtaccaa catgacgttt ctcaaaaacg tttggctgaa
     4021 caattggatt ttgttgtgga aacagcggtt aaccaagtag gtgtggatgt gaatacagca
     4081 agcccgcagc tcttgcagca tatatcagga ttgaataaaa caactgccca aaacattgtc
     4141 atttatcgtg aagaaaacgg agaattcacc gcacgaactc aattgaaaaa agtgccacgc
     4201 ttaggaccaa aagcatatga acaagcaatc ggattcttgc gagtccctgg tgggaaaaac
     4261 attttggaca acacaggtat ccatcccgaa agttattcta ttgcaaaaga aatattaact
     4321 tcagtccaac tttcagaaaa agagcttggt acagaagaag cagtagaaaa attgacacga
     4381 ttatccgtag agaaactggc tgaaagccta tcagtcggag aagaaacgtt agccgatatt
     4441 ttagctgggc tgacacaacc tggacgagat atgcgtgatg agatgccagc gcccttatta
     4501 cgtacagatg tattgagtat ggaagacttg aaaccaggga tggaattgac tggaacggtc
     4561 cgtaatgtca ttgacttcgg cgcatttgtt gatatcggtg tgaagcaaga cggacttgtc
     4621 catatatcga aactcagcaa aaaattcgtc aagcatccta cagatgtcgt gtcagtagga
     4681 gatatcgtaa cagtttggat agaacaagtc gatacgaaaa aaggcagaat cagtttgacg
     4741 atgctttccc cttatgaaga gtaacaatga ggagtaacaa aaattgacac aagaagaact
     4801 gcagcagtta gtagaagaga tatcattaaa aagcttccag cttcctttca agcaccaagc
     4861 aatgttcaat ccacgcttac gtacaactgg cgggcgctat catttaaacg atcatcattt
     4921 agattttaat gcgaagctat ttgaagagtc agacgaagag acgattgcag gaatcatcaa
     4981 acatgaactt tgccattatc atcttcatct agctggaaga gggtatcagc ataaagatat
     5041 ggatttcaag tatcttttga aaaaaacagg agggttgcgc tatgcaccag ccttgacgaa
     5101 gaaacagata aagatggagt attatcgatg ccaagattgt tctgccacga tctgccgcaa
     5161 gagaagaatc gatgtacgca aatatcgttg tggaagatgt catggagaaa tcaaatggat
     5221 tgagacaaaa agagaagact ttaatgaagc acaaaaaatc agatgaaagg atagaaggcc
     5281 tttggaaaaa agtgagcaga ggaatctaaa agactggaat cagctattaa attttttagc
     5341 cgcacctgaa gagggaaatt ttgaggaact gacggcacct acacttattt tagctggaaa
     5401 ctgtcttcct atcttagctg ataaagcagc acaaatgtat ttaaatggcc aagtagatca
     5461 attgtttttg gtcggagggg tggggcatgc gactagaatc ctttatgaga attttgaaaa
     5521 gcaaggattt cattttgaag atggcatgag tgaaagtgaa atctgtcgtc agtatctaaa
     5581 agaagtctat gatttgcccg ataaggcttt tctgatagaa tcgaagtcta caaattctgg
     5641 agagaacgcc actttttcac tggagattct tcattcctta ggagctgtac cagaaaaaat
     5701 cttattgatg aatgatccga cactgcaacg caggactcga gcaacttttg agaaagtatg
     5761 gcaaaatgaa caaactattt tcgcgaatgc tgtgccattt gtgccggaaa tccttcactt
     5821 ttctgaagag atcatcttta cagcaaaaga actgaatcat caatggccta aggaatattt
     5881 ccatgcgcta gtattaggag aaatggaacg tctccatgac gatgagaacg gctatggtcc
     5941 gaaaggaaaa gattttattc ctcacattga tatatcagag gaagtttggt cttcttacac
     6001 cagaattaaa gagtcaataa aaacggattt ttctcgtact tgaccatttt agcaagcgaa
     6061 tcctcctttc caaataaatt tggttgaaat tttcaatcgt ttataagata atgaggagcg
     6121 tgttgaatgt aatagctaaa agaatggacg ctagataaga gaagaggatt caaatggaaa
     6181 atcaaaataa attgtatcat tttgttggta tcaaaggttc aggaatgagc tccttggctc
     6241 ttgttttaca tgaacaaggt ctaaatgtcc aaggatcaga cattgaaaaa tatttcttta
     6301 cacaaagaga tttagaaaaa gcaaatatca cgattctgcc atttaacgca gataatgtaa
     6361 aaccagggat gacgatcatt gcaggaaatg catttcctga ttcacatgaa gaaatccagc
     6421 gagcaaaaga attaggtttg gaagtgattc gctatcacga ctttattggt cattttatcc
     6481 aaaattatac gagtatcgct gtaacaggct ctcacggaaa aacaagtacg actggactgc
     6541 tctctcatgt attgagtggt gtccgtccaa caagttatct aattggagat ggaacaggac
     6601 atggtgatcc gcaagcggaa ttcttttcat ttgaggcctg tgaatatcgc cgtcatttcc
     6661 ttgcctattc accagactat gcaatcatga ccaatatcga ttttgatcat ccggattatt
     6721 atacaagtat cgacgatgta tttacagctt tccaaacaat ggctggacaa gtgaaaaaag
     6781 ctatttttgc gtatggtgac gatgcttatt tacgcaaatt aaaagcaaat gtgccgattt
     6841 attattatgg tgtcacagaa aacgatgata tccaagcacg aaacatcgaa cgcacaactt
     6901 ctggttctgc ttttgatgtc tatcatgggg atgaatttgt tgggcacttc actgtaccag
     6961 cattcggtaa acacaatatc ttaaatgctt taggtgttat tgcagtagct tatttcgaaa
     7021 aactggatct gaaagaagtt gcagaagaaa tgctgacttt cccaggagta aaacgtcgat
     7081 tcagtgaaaa gatcgtagca gacatgaccg tagtcgatga ttatgcgcat caccctgcag
     7141 agatcaaagc aacgattgat ggcgcacgcc aaaaatatcc agataaagaa ataattgcag
     7201 ttttccaacc ccatacgttt acgcgtacga ttgcattgat ggatgaattt gcagaagcac
     7261 ttgatttagc tgataaagtt tacttatgcg atatttttgg ttcggctcgg gaagaacaag
     7321 gaaacgttaa gattgaagac cttggagcga aaatcaaaaa aggtggagaa gtcatcaaag
     7381 aaaataatgt atctccattg ttagattatc atgatgcagt agtaatcttc atgggtgctg
     7441 gagatgttca aaaatttgaa caagcgtatg aaaaactttt aagcagcacg acgaaaaatg
     7501 tactgtaata caattactgc agtgtcctta taaaaataga aagaggggga gtggcaaaaa
     7561 tcttgccaag cccccttttt tgcaacttat gataaacggt gttcaaaagc ttgcatctgc
     7621 ggtaataagc ccaatcaaat caaaaacatg agaagctgtt ttttcttggc tgttcttatt
     7681 atttgatgca ggacagcttt ttcacgacct ctgtaaacta taggaatctg ttaaatcaag
     7741 ggagcagaac ttgcctttta cgctactttt ggtacaataa tgtatgatta aaaataaact
     7801 ggagggtttc tatcttgaac ataggtaagc cgagaaaact aggaaaaaca ataaatgaaa
     7861 tcgaggaagg cgactcgtta tccttaacag aatctattga agataaccag cttttgctgt
     7921 atttgggatt gacgaatgat gcaaatcctt tgtatatcca acatgacttt gcacaaaaaa
     7981 cagaatatca acagccaatc gttccatcag tgatgctgat ggggatcatc acaagtgcaa
     8041 tttcaaagca tcttcctgga ccaggatcac atgttgtcaa tttttctgtc aattttattg
     8101 aaccggtttt ccattatgaa acactgactt ttcagtttga ggtcataaaa gtagataaga
     8161 tgaaggatgt ggtgacgatt tctgtagaag cggagaatac gaacgaagat cgtgtgttgg
     8221 atgctgtcgt tatggtccaa ccgccacaag ttcctgaatt ggaaatagca atcgaaagtg
     8281 aaggtgaatt gaatgaataa tccaagtaca tcaatgtcag gtatcaatcg tttttatgca
     8341 aaagtttatg gatttttagg tatgggactt gcccttagtg cagtcacttc ttacttggct
     8401 ctttgggtat ttcctttgca attagcaact tttatcaata attttccact aggctttgtg
     8461 ggtctgtgga tcgttgaact gattttagta atggttctag gcgtaaaagc acaaaagaat
     8521 ccgagcttag caatcggtgg atttattgcc tactctcttt tgaatggcat aacattagcg
     8581 gttactcttg catactacga tattggaaca gtaacgcaag ctttcttgac agctactttg
     8641 atgttcatgg ggatgtctgt tgtcggagta acaacaaaaa gagatcttag cggggttgga
     8701 agagcaggac aggcagcttt aatcggagtg atcattgcac tagtcttgaa catcttcatt
     8761 ttgagaagta gcgtagttga gctgttcatc tccctactgt tagtcgtcat ctttgcaggg
     8821 attacagctt atgataacca aagcatccgt aatatctatg tccagacaaa cggaacagca
     8881 catagtggaa ttgctgtatt catggcattg aagctgtatt tggactttat caacttgttc
     8941 ttggcgtttc tgagaatctt tggacggaac aactaaaaaa gaaaaaaatt catcagttgg
     9001 ggctataccg aattatttgg tatggtcccg ctttttcttt tcgcattccg caggattctt
     9061 tgttagaatg aataagaact ttacagtaag gagttaagaa gatgacaaaa aataaattat
     9121 tactcgttga tgggaacagt gtggcttttc gcgcattctt tgcgcttcac aattctcttg
     9181 aacgtttcaa aaatagaaac ggtcttcata caaatgcaat ctatgcattc aatacgatgt
     9241 ttgaaaatgt tatgcaaaaa gagcaaccga ctcatgttct ggttgcgttc gatgcaggaa
     9301 aaacgacttt tcggacagaa atgtatgcgg agtacaaagg tggaagatcc aaaacacctg
     9361 gcgaattcaa agaacaaatg ccatatatac gtgatttgtt gacaggacta ggggtacagt
     9421 attacgaatt gcccaattat gaagcagatg atattatcgg tactttggca gaaaaagtag
     9481 acaaagatca atttgatgtc gtcgtgcttt caggtgatcg ggatttgact caattagcta
     9541 gtaaagaagt aaaagtggat atcactgtaa aaggtgtcag cgatatcgaa agctatacac
     9601 cagaacatgt agcggaaaaa tatgatgggt tgacgccaaa acagattatt gacatgaagg
     9661 gactagccgg agatgcctct gacaatatcc ccggtgtgac taaaatcggt gaaaaaacag
     9721 ctatcaagct attgaaacaa tatggctcag tcgaaggaat ttatgaacat atcgatgaaa
     9781 tgaagcaaag caaaatgaaa gaaaatctga taaatgacaa agagcaggct ttcttatcga
     9841 aaaaattggc gaccatcaat acttcagcgc ctgtggatgt ctctatcgac tcattgaagt
     9901 atgaagggaa aaatctcgat aaactcgttc cgttttataa agaaatggat ttcaatcagt
     9961 tcctttctaa attgaatatc gtagaagaag aagtaaaaat ggatgatatt ttgtttgaag
    10021 tcgttcatga attcaaagag gaaatgttta ctacggatat ggcgttgtac gtggaaatga
    10081 tgggcgacaa ttatcataca gaggaaatcg tcggagtagc gtgggggaca gaaaagaaaa
    10141 tttatgtcac gaacgatcta tctgtattcg aaaaccgagc gttccattct tggatcactg
    10201 atccgactcg gctgaaaaaa gtatatgatg cgaaacgaac ttatgttgct ttaaaccgct
    10261 atactggaaa aacgaaagga atcgattttg atgtcttgct aggtgcatac cttctcgata
    10321 cgaatgaaaa aagcacagat atcgaaggaa tagctgctca ttacggatac aacgatatcc
    10381 aatcagatga agctgtatac ggcaaagggg cgaaaaaagg tttaccagaa gaagaggaac
    10441 tcttttttgc tcatttagcg cgaaaagtag ctgcaatcaa tgcgttgaca ccaaaattga
    10501 gccaagaact ggcagaaaaa aaccaagaag atcttttccg gaaaatggaa ctgccattgt
    10561 ctatcatttt ggcagagatg gaaataagcg ggatcaaagt agatgcgact cgcttacaag
    10621 aaatgaaggg cgagttctct gctcgcttaa gagagatcga acaaaaaatc tacgaagaag
    10681 ccggcgaaga attcaatttg aattcaccaa agcaattagg agtgatctta tttgaaaaaa
    10741 tgggattacc cgttatcaaa aaaacaaaaa ctggatattc cacagccgta gatgtactgg
    10801 aacagcttcg tgaacaagca ccaatcgtag aggatatcct cacctatcgt caaattgcaa
    10861 aaattcaatc tacctatgtc gagggactgc tgaaagtgat ccaatcagat ggaaaagtgc
    10921 atacccgtta tgtccagaca ttgacccaaa caggacgatt aagctctgtt gatcctaatt
    10981 tgcagaacat cccgattcga ttagatgaag ggagaaagat acgtcaggca tttgttccta
    11041 gagaaaaaga ctggctgatt tattcttctg actattcaca aatcgagtta cgcgtacttg
    11101 cgcatatctc aaatgatgaa catttgaaag ctgccttcct agaaggtcaa gatatccatg
    11161 ccagcacagc gatgcgtgta tttggaatcg aaaaggcaga ggatgtcaca ccaaatatgc
    11221 gtaggcaggc aaaagcagtg aattttggta tcgtgtatgg catctctgat tacggacttt
    11281 cacaaaatct aggaatcagc cgaaaagcag cacagcaata catcgatacg tactttgaaa
    11341 aatacccagg tgtcaaagaa tatatggaaa gaatcgtacg cgaagcaaaa gaccaaggct
    11401 acgtcgaaac gctgtatcac cgccgtcgat atctgccaga catcaattca agaaattaca
    11461 atatccgttc ttttgcggaa cgaacagcta tcaatacacc aatccaagga agtgctgctg
    11521 atattttgaa aatcgcgatg atcgaattgg ataaacgatt aaaagaaaca ggtcttcaag
    11581 ctacgatgct gttacaagtt catgatgaac ttgtcttcga ggtaccggaa aaagaacttg
    11641 aatcattaga taaattggtg aaggaagtta tggaacaagc tgtttctctt catgtaccac
    11701 tgatcactga cagcagttgg ggaaaaacgt ggtacgaagc aaaataaaca ttgtttaaga
    11761 cagagcaata taatgaaggt gaaaagaaaa aatgccagaa ttaccagaag tagaaaccgt
    11821 tcgtaaagga ttggaacggt tagtagtagg aaaaacgatt caaaaagttc aggtcttgtg
    11881 gcccaagatc atcgagcaac cagagacacc catctttgag gcttctcttg ttggcgaaac
    11941 catccaatcc attggacgta gagggaagtt tttaattttt catctcgatc attgtgaact
    12001 gatctcacac ttgcgaatgg aaggaaaata tcaatttacg aaagaaaata caccaatcga
    12061 taaacatacg catgtacttt tcttttttga ggatggaagc caacttcgct acaacgatgt
    12121 acgaaaattt ggtcgtatga caatcgtcga aaaaggagca agtgcaactt accgaggaat
    12181 catgaaattg ggacctgagc ctttgccaga tagcttctta ttagcagatt tcgcaaatgg
    12241 actgaaaaag tcccacaaag caatcaagcc attgctgcta gaccaacgtc tagtgactgg
    12301 tctaggtaat atttatgtag atgaagcatt atgggaagcg aagatccatc cagaacaacc
    12361 ggcgaacaca ctgcgcccca aagaggtaga acaattacgt ctatcgatca ttgatgtttt
    12421 ggatcgtgca atagaagccg gaggtaccac gattcgcagt tatttgaatg cattaggaga
    12481 atcaggcgga tttcaagtag cactgcatgt ctatcagcaa actggcaaac catgtatacg
    12541 ttgtggaacg ccgatcgtga aaacaaaagt ggcacaaagg gggacgcact attgtccgaa
    12601 ttgtcaaagg ctaaaaatcg caaagtagga tttgttttag gtttgacggg tggaatagct
    12661 accggtaaaa gtacggcagc caaagtcttt cagtctcatg gatttccttt gatcgatggc
    12721 gatgtgattg ccagagaaac tgttgagcct ggaacgcttg ctctgaggaa aattgtatca
    12781 gtgtttggcc aagaggtatt gcagcctgat gggcaactag atcgagggaa acttgggatg
    12841 attgtttttc catcaaagga aaaacggcaa aaacttgatc agctattaga tccgtttatc
    12901 cgaaaagcga taaaagatca aatcgcctct ctaagttcca atcatccttt agtaattgta
    12961 gatatcccat tgctctacga aggtcattat gaccactata tggatgcagt agctgtcgtt
    13021 tatactacgc cagaaactca gcttcagcgt ttgatgaaac gtaatcaact gacgctagaa
    13081 caagcgcagc agagaattag tagtcaactt ccaatcgaag aaaaaaagca gcgtgcggat
    13141 attttgttcg acaacaatgg aacaaaagaa atacttgtgg gacaaatcga aaattggctt
    13201 tttgctaatc gctttctttg agtatctgtg tcaattttgc tatcctgagt atatagaaag
    13261 tgagggatag cgatgggatc attaaaaaat caaaaggata aagtcgtagg taacgcaaaa
    13321 gaaaaaatcg gcgagatgct caataaagaa aatctaatcg atgaaggtcg tacgcaatca
    13381 gaaacagcag aagaaaaaga aaagcagtac caagcaagaa tcgatgaaat agaagaccag
    13441 caggcagaag aagaagcgaa gcggcaagat ttgactcaag aacgaaaaaa tccgacacct
    13501 gatcgtagta aaacaccgct cccagaaagt gaccacattc ccgatgagca aaaaatcgag
    13561 gaacagcgaa tgaaacacta tacaggaatc gaagagaagt tataaaatag taaaaaatcg
    13621 agatgtgact tgaagcagat gagaaggtgt tcatctgctt caagtttttt gtgctagatg
    13681 actaaatcag cttttgtgta agttatttct cagtaatcaa ggtgatcagt attggaaaat
    13741 tgaaatttag taaggacagg ggacacaatt acgcatgaaa gtagaaaaaa ttgttcttat
    13801 atccgtgttt tttgggtagg acagtcgcgt ttgtgatttg tttcgtgtta taatacaact
    13861 agttgtaaag aaagacacat cctttatata aaatgcgatg caagaggtga gacaaaatga
    13921 gatgtcccag atgtcaacat aataattcac gagtaatcga cagccgtcaa gcagatgatg
    13981 gacgtgcgat ccgtcgccgt cgcgaatgtg agaattgttc gttccgattc actacatttg
    14041 aacgaattga agctgctccg ttattagtaa tcaagaaaaa cggggaacgc gaagagttca
    14101 atcgcgataa aattttaaga ggactcatcc gttcagcaga aaaaagacct gtagctatgg
    14161 aacaaatgga acaaatcgtc aaccatgtcg aaaaccgcgt acgcagtctc ggagaaaacg
    14221 aagtatcgac gaatctaatt ggtgaatatg tgatggaaga tttagttgat ttagacgaaa
    14281 tcgcttatat tcgttttgca agtgtttatc gccagttcaa agatatgtct gttttcttaa
    14341 aagagttgca agatatcgtg gataaagcaa aagaaaataa taaagagaat aataaagaaa
    14401 acaattaaga aacaaaaagg aggcatttcc ttggaaaatg cttggaaaga gcttcaacca
    14461 aaaaatattt accaaggaag aaaagcggct ccgctgagag aagacagcaa gaacgccttg
    14521 ctgtatcttt atcagccact aatcggcgga gaagccttga gtatctattt caccctgtta
    14581 tcagaaatct ctcaagaatc tggcaaaggt cctgaaggga tgcatgcaga tctgcttagt
    14641 agtctaggct gcggtatccc gcaattttat gatgcaagaa aaaagctaga aggaatcggc
    14701 ttactggatg tttattttaa agaagatagt gatctgggac ctcgttttat ttatgagctg
    14761 ttagaaccac tcattcctaa agcatttttc aaagactctg ttctggcttt tcttcttctg
    14821 gaaaaagttg gtgaacgacg attccagaag cttacccaat tctttgaacc taaaaggcat
    14881 acagcagagg gatatcaaaa aatcacaaaa aaatttttag atgtttatcg cttcaatgaa
    14941 gcagtgtacg ctgctaatca agaaaacatc gaatcgctga ataaatcttt tgaagaatct
    15001 gaatcagtag cattacttga agcagattcg acattcgatt ggcagttttt gaatgattgg
    15061 ctggcaaaaa aacatattgc aagtcttgat gatccaatca tcaaacaagt cgacatgtat
    15121 catcagcttt acggtttcga tgaattgaca cttggcgaac tgatcagtca atcttatgac
    15181 tttagtgaac aaaaagtatc tgaaaaagaa ctgcaaaaag ccattttggc aatgcagaga
    15241 cgtccggtga agcaaacaaa agaaacagtt gcggtttctg aaaacagtaa tgaaccctcg
    15301 tcaaacgaac atttgacaaa cgcagcgctt cagctgataa aagaagcaca gagcattcca
    15361 ccgatgaaat atctggaagc aatcaagtca gaaaaaggcg gatatgtttc taaacaagaa
    15421 aactggttac tgcaagatct tgtttctcaa tcaggtcttt ccagtagcgt gatcaatgtt
    15481 ttgctgaatt atgtactagt gatcaagaat aatgctagct taaatgccag ttatgtaaat
    15541 acagtggcaa atgaatgggc acaaaagaaa atcgccacag cagaagaagc gatccagcac
    15601 attcggcaaa taagtaatca agcaaaacaa tcaaaacaaa agaagcaaac aaattatcaa
    15661 actggcaagc gaaatgttcg acgggaaaag cttcctgatt gggtcaacca gccaaaagat
    15721 gaaaaacaga tcagcagcga gaaaaaagca gagattgacc gccgttttaa agaatacctg
    15781 gcaaaaaagg agggtgatat ctagtggaag acgtaggcaa agaattgaag aggatcatca
    15841 gcaaacgcaa ttatcaagaa aagtatgctg aaatgatcgc tgaagtctta aatgatgcgg
    15901 atgttcaagc ttttctaagc gcacaccaag aagaattgac acaagcggat attgaaaaaa
    15961 gttacgctaa attgtatgaa ttcgtgcaag aaaagcgtaa atatcaaaaa aacgatccaa
    16021 cgatgatcgc tcccggctat gagccgaagc tgacgttgaa ttttcattat gtggatgtca
    16081 cttatgtacc aacggaagca ttgttagcac ggcaaagaca agaagaaatc agaagccgag
    16141 taaaagcaat ggatatgcca aaagatattc aatcggcttc ttttgaaaac tttgaacgta
    16201 cagatggacg aggatatgcg agcttagaag ccctagattt tgtagagaaa tatactgctg
    16261 atccaaaagg gtatcataaa ggactgtatc tagtaggaag ttttggtatt ggaaaaacat
    16321 atttattagg agcaacagcg agagaattag cgattgctgg attcaccacg acgctagtcc
    16381 attttccgac atttgccgtt gagatgaaac aggcaatcgg aaaagatcaa gtggcagaaa
    16441 agttagatgc agtgaaacgt tcacctattt tgatgatcga tgacattggt gcagatgcga
    16501 tgagtagttg gatacgagac gaagtattcg gtgttatcct tcaatatcgg atgcaggaac
    16561 agttaccaac cttcttctca tcgaatttta caatggatga attggaacag catttgtccg
    16621 tcactcaacg tggagatgaa gagccgctaa aagcgaaacg gataatggaa cgcatacgtt
    16681 atttgacaaa agaaatcgaa atgactggtc gcaaccgacg caatggttga acagttgaca
    16741 agtccaactg acatttgacc tagtagtaaa agcaggtcag cgaatcatta tatggacaga
    16801 aatgaccgga gacaatcacc gcaacaagta atgcagaaac ttaaacggag gattttttat
    16861 gagggctatt ttttctagta tatctgtagt tggtctattt tttcttttag caggctgcgg
    16921 tagcgcagat agcaacacag gttctgttga ttcgattgat gcgggtcaaa cagtaaaaac
    16981 aagcagtacg aaaacggaga gacaaagcat atttgtcgga acgattgaaa gtatcactca
    17041 agtagaagca gaagaagaaa cgcttcagtt gaaactcgtc gatgtcatgg ccaaagaaga
    17101 tccagcgaat atgggaacgt cattcagcaa tgacggagtg atcttaaatg ctactgctga
    17161 aaaaataagc gtggacataa atgaattgaa aatcggtgac aaagtccaat ttacattagt
    17221 tgagatgccg atcatgacga tgtctatccc accgcaagtt cctggagatt cgattattga
    17281 tgtttcattg aacaaataac caataacatt ttttcaaaaa taagaaaatg ttgttttata
    17341 gaacgaaaag gtatattttt taaggagata tgggaatttt tttattccta gtctccttat
    17401 tttatttgga aaatgctata attatctaga aggagtgaag ctatgaacca tttagattta
    17461 tctcagcgct tagccgcagt gggtgacttt gtccctgaat ccgctcgttt agcagacatt
    17521 ggatcagatc acgcttactt acccgttgca ttgatattaa gaggaaaaat cgaatatgca
    17581 gttgctggtg aagtcgttca aggtccttat gaatcagcac gtaagcaagt caaaaaagat
    17641 ggattagaag accgtatcac cgtacgcctt gcaaatggtc tagatgctat ccagccacaa
    17701 gatggtatta cagctgtcac gattgcgggt atgggaggtt cactgatccg agatatccta
    17761 gaagctggca agttgaatca acgcttaagc ggacaagaac gtttgatcct tcagccaaac
    17821 atcggagagc gtacattacg atcttggctg caagaaaatc agtataaaat ccaatcagaa
    17881 aaaatacttg aagaaaataa aaaaatatat gaaatcattg tagccgaaaa agaacaaaaa
    17941 ccagttactt acaatgaaat ggagctgatg ttcgggccat ttctgcttga agagaaaagt
    18001 gagatcttca aatcaaaatg gacaagagag cttcaacaaa gaaaagctat ccgtgaacag
    18061 ctcaaacaag cagctaatca agaggcacgt caaaaacaaa tcgagcagga aatccaaatg
    18121 attgaggagg ttttaaagcc atgacaatca gcggacgcac tttcatccaa aagtttgaaa
    18181 gctattgtcc tcagtggcta gctgaagaag gagacccagt cggactgcat atcggaacat
    18241 tggataagcc aatccaacga gtaatgatga cattagacgt tcgtccagaa gtggtcgaag
    18301 aagcaatcaa gaaaaaaatc gatttgctga ttgctaaaca tcctccaatc tttcgtcctg
    18361 taaaacggct ggttactgat cagccacaag aaaaaatgta tgcagatttg ttgaaacatg
    18421 atattgccgt ctatgcagca cacacgaaca tggatattat ttgggatgga ctgaatgact
    18481 ggttttgtga acttctcggt attgaagtgg agagttattt agtaaagact catgagattc
    18541 attataaaaa actggccgtt tatgtacctg tggatcatgc acagaaaatg cgtgaagtat
    18601 tagctgcagc tggtgctggt acacaaggtg attataccgg tacaagtttt acctcgattg
    18661 gacacggtcg atttactccg gaagctggtg cacaaccagc gattgggaaa gtagggaaga
    18721 cagaacaagt tcaagaggca aaagttgagg ttattttgcc ggaaactatc gaaaaacaag
    18781 tgattcaagc catgcggtca gctcatcctt atgaggaacc tgcttatgat cttttcgcta
    18841 tagatgaacc agtcgaaatg tttgggttag gacgagtagg tgaattgccg caagaaattt
    18901 ctatagaggc atttgtagaa caagtaaaag aagcgttcca actagatgga ctgagaatag
    18961 tccagccaaa aaatgcaaaa tcctctgtga agcgcattgc gatttgcggt ggaagcggtg
    19021 aaaaatttta tccgcaagca atcgcccaaa gagcagatgt ctatatcacg ggagatatct
    19081 attatcatac ggctcatgat atgcaaagcg ctgggttgat tgcaattgat cccggtcatt
    19141 atattgaatc gttatgcaag caacgattta tcgagaaatt tgaatcttgg aaacaagaag
    19201 aaaattggga tattaacttt ttcgtttctg aaacaaatac aaacccattt caatttaatt
    19261 aaaaggagag aatactatgt acgaaaatct tttgccacgt tttttacgat atgtcaaaac
    19321 agaaacacgc tccgatgcaa caagcaaaac gactccatcg actcagacac aagttgcttt
    19381 tgcgcaagaa ttgaaaaaag aattagaaga gttaggctta gctgatgttt tctataatga
    19441 agataacgga ttcgttatgg gaacattacc aagcaatgtt gatcatgaag tgcgttccat
    19501 tggatttatt tcccacatgg atacagcaga ttttaatgca gtaggagtca atccacaaat
    19561 cattgaaaac tatgatggcg agtctacgat cgtactagat gcggaagaac gctatacatt
    19621 gaatacaaaa gattttccaa atttgaaaaa ttatgaaggc cacacattga tcacaacaga
    19681 tggcagtaca ttactgggag cagatgacaa atcagggatt gcggaaatca tgactgcaat
    19741 ggagatcttg atcaataatc cttctatccc tcacggtacc atcaaagtgg cttttggtcc
    19801 tgatgaagaa atcggtgtcg gagcggataa attcgatgtt gagcaattcg atgtagattt
    19861 tgcttataca gtcgatggtg gtcctttagg tgagttgcag tatgaaacgt ttagtgctgc
    19921 tcaagcaaat attacgatcc aagggaaaaa tgtacatcca gggacggcaa aagatacaat
    19981 gatcaatgct ctacagttag caatcgattt tcataatcag cttccagccg atgaagttcc
    20041 agaaaaaacg gaaggatacg aaggtttctt ccatttgatg gcattggatg ggaatcctga
    20101 agaagcttct atgagttata tcattcgcga tcatgatcgt gaaaaattcg aagcacgcaa
    20161 agcacaaatc acacatatcc aagaagagtt gaatcagcga tttgatcaag aacgaatcaa
    20221 agtagatatg tatgatcaat attacaacat gaaagaaatc attgaaaaag atatgagcat
    20281 cgtggaatta gcgaaacaag caatgatcga gcttggtatc tctccagtga tcgaaccagt
    20341 tcgtggagga acagatggtt caaaaatctc ttacatgggt attccgacgc caaatctatt
    20401 tgccggagga gaaaatatgc acggacgatt cgaatttgtt tctttacaag taatggaaaa
    20461 agcaacagac gtgattgtaa aaattgcaga attgaacaca aagagttaaa atcgaaaaag
    20521 aacagcttat gacaaggcgt tcgtcccaat gatggaatgt catctgtcta aggctaaagg
    20581 gcaggacaat ggaattggtt gtcccgccct cttttttgaa gagtaaatag gaggtaaaga
    20641 atggaaaagc tgctagtttt ttataacaaa agttcaggga aagatgaggg tgagaaatta
    20701 gcacgctggt ttcaagatta tgtaaaagaa aagcagccag aattatatgt agaactagct
    20761 gaaactggcc catcgattga tgatgcggaa ttagtcaaga aagcagaagc catagaagct
    20821 gatactttag taattatagg tggagacggc acgattcatc atatcgtcca agcgttccaa
    20881 aaaacattat ctcaatacca tgtcggatta cttccaggtg gaactgtcaa caaccttgca
    20941 cgtgttcttg agattcccct caaaaaagaa gaagctgctg acgtgatcct ggaaggcaat
    21001 gtacggaaaa tagactatgg tcaagtcaat gatgaagtca ttatctctac actgacaatc
    21061 ggaatcttag cagatacagc aatttggatc agtcaagaag aaaaacagcg ttacggctcg
    21121 tggatcttta tcaagaagtt ttttaagtta ttgctgaaga agaaaaaata tcatttggat
    21181 ttagaaacag acgaggagca ttggaaagga aaatcccaac tgttgacagt cacgatgtct
    21241 aactccgtgg gcggttttac aaattttgac gactcagcga ccctagatga tggaaaattt
    21301 catgtaacga tcgtaccttc cttagatatt ttccgattta ttttttattt gcctagaatc
    21361 atgagaggca aattctatac gatccccggc attacttatt tcacagccaa acgcatgaaa
    21421 atccaagcgc aggaaaaatc tgtgggcact cgaacggatg gagacacgac agatgatctt
    21481 ccagttgaat tatctgttgt atcaaaagga ttaacagttt attcaccaaa gaaagaagaa
    21541 aaagcttaga aatagtagtt aacagaaagt tggaagaaga gtaatggatg tattgttaca
    21601 caatcctttg tggttttacc ttttcattgt gaatttttac ttattttgtc tgatggggta
    21661 tgataaacat caggctagaa ctagcggctg gcgtgttcct gagaataatt tgatatttgt
    21721 cagcctgtta ggcggtggtt tgggaggatt gatctctcga aaagttttcc atcataaaac
    21781 tcggaaaaag aagtttacta taggattcgc tgcaggtgta cttgttgatt tgattctcat
    21841 ttattttttc cattaaggag caggagatgt cgtctaaagg aacgaaaact aaaatcattc
    21901 tgaatctgct tttgctgatt gtgatttttg ccattattta ttatttaatc aatcaatcat
    21961 ttgcagatat ctttgccgaa ttgctgtcta ccagtgttgt tgttttggca gcggctgtta
    22021 tattagggac agtttaccaa atcgctgaag ggcgctcaat caaagaaatt gctgaacctt
    22081 tcgcgccgaa ttttacaacg aaagatgggt tcttgacctc tagctatatt gcgttttacc
    22141 gaatcgtttc gtttggtaca ggaacgctga tctcggagat ttatttttat aacaaaaaag
    22201 gaatgaagct atcccaggga gcaggggtga cagcactcca tatgattatg tacaaactgg
    22261 cagtgatcac ttatgcatta gctggcttgg tcatccaatt ctctcttttt tattctaatg
    22321 gtccatcaat gatctggttt atcatcgctg gtatcctttt gacgatcgtg attgttgcct
    22381 ttttattggc gatgtccgtc agcttgaatg ttcaaatatt ttttgtcagc ttatcgaata
    22441 agttatttaa aagtgagcgt ttgcggaaat gggtggatac atgcaatcag cagatctact
    22501 ctttaaggga aacggtccaa accattatcc aagacaggac tgcgctattg cggatttatc
    22561 tatggaatat gttaaaactt gtattttggt atgtacttcc ttatttgatt ctgattgaaa
    22621 accatcctaa aatagatttt ctactgacca tttcgtttat cagctttgca gtagtgcttt
    22681 caggggttat tcctacgcct gctggtattg gttcctttga atttgtctat ttgttattat
    22741 tcagaccagt tgttggaaca gttgacgcag tttcttcgct tttactgtat cgtttcagtt
    22801 cctttatctt gccatttctc tatggatttg tgtatgtgat tgctgaacgg cgaagagtga
    22861 tcaaagaaga aataatagaa gtaaaaaaag a
//
LOCUS       DAKBVG010000017        22808 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000017 DAKBVG010000000
VERSION     DAKBVG010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 22808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.017"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            107..517
                     /locus_tag="OUD09_000533"
     CDS             107..517
                     /locus_tag="OUD09_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCT9691832.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            510..1211
                     /locus_tag="OUD09_000534"
     CDS             510..1211
                     /locus_tag="OUD09_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HCT9691833.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            1211..2797
                     /locus_tag="OUD09_000535"
     CDS             1211..2797
                     /locus_tag="OUD09_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCT9691834.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            2816..3037
                     /locus_tag="OUD09_000536"
     CDS             2816..3037
                     /locus_tag="OUD09_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HCT9691835.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            3034..3795
                     /locus_tag="OUD09_000537"
     CDS             3034..3795
                     /locus_tag="OUD09_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HCT9691836.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            4175..4684
                     /locus_tag="OUD09_000538"
     CDS             4175..4684
                     /locus_tag="OUD09_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HCT9691837.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(4759..5298)
                     /locus_tag="OUD09_000539"
     CDS             complement(4759..5298)
                     /locus_tag="OUD09_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HCT9691838.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(5438..6049)
                     /gene="rpsD"
                     /locus_tag="OUD09_000540"
     CDS             complement(5438..6049)
                     /gene="rpsD"
                     /locus_tag="OUD09_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCT9691839.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            6311..7162
                     /locus_tag="OUD09_000541"
     CDS             6311..7162
                     /locus_tag="OUD09_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCT9691840.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            7190..7825
                     /locus_tag="OUD09_000542"
     CDS             7190..7825
                     /locus_tag="OUD09_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HCT9691841.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            7845..8618
                     /locus_tag="OUD09_000543"
     CDS             7845..8618
                     /locus_tag="OUD09_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCT9691842.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            9026..9823
                     /locus_tag="OUD09_000544"
     CDS             9026..9823
                     /locus_tag="OUD09_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HCT9691843.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            9801..10214
                     /locus_tag="OUD09_000545"
     CDS             9801..10214
                     /locus_tag="OUD09_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HCT9691844.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            10198..12843
                     /gene="mutS"
                     /locus_tag="OUD09_000546"
     CDS             10198..12843
                     /gene="mutS"
                     /locus_tag="OUD09_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCT9691845.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            12861..14969
                     /gene="mutL"
                     /locus_tag="OUD09_000547"
     CDS             12861..14969
                     /gene="mutL"
                     /locus_tag="OUD09_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCT9691846.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            14991..15551
                     /gene="maf"
                     /locus_tag="OUD09_000548"
     CDS             14991..15551
                     /gene="maf"
                     /locus_tag="OUD09_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HCT9691847.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(15636..17624)
                     /gene="treP"
                     /locus_tag="OUD09_000549"
     CDS             complement(15636..17624)
                     /gene="treP"
                     /locus_tag="OUD09_000549"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HCT9691848.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            17847..18560
                     /gene="treR"
                     /locus_tag="OUD09_000550"
     CDS             17847..18560
                     /gene="treR"
                     /locus_tag="OUD09_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HCT9691849.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            18637..19083
                     /gene="msrB"
                     /locus_tag="OUD09_000551"
     CDS             18637..19083
                     /gene="msrB"
                     /locus_tag="OUD09_000551"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCT9691850.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            19253..19855
                     /gene="ruvA"
                     /locus_tag="OUD09_000552"
     CDS             19253..19855
                     /gene="ruvA"
                     /locus_tag="OUD09_000552"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCT9691851.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            19868..20869
                     /gene="ruvB"
                     /locus_tag="OUD09_000553"
     CDS             19868..20869
                     /gene="ruvB"
                     /locus_tag="OUD09_000553"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCT9691852.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            20898..21476
                     /locus_tag="OUD09_000554"
     CDS             20898..21476
                     /locus_tag="OUD09_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCT9691853.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            21528..22010
                     /locus_tag="OUD09_000555"
     CDS             21528..22010
                     /locus_tag="OUD09_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HCT9691854.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            22127..22501
                     /locus_tag="OUD09_000556"
     CDS             22127..22501
                     /locus_tag="OUD09_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9691855.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7423 a         4077 c         4930 g         6378 t
ORIGIN      
        1 aaaacgtttt ttttctcaaa aattgtatct tagaaacagc atgttttgaa actttcagaa
       61 aaggtttgtt agtaaaatga aagctatacg taaggagtgt tgtttgatga aattttttca
      121 agagtttttc attattattt tcttttctgt attaggagaa ggcatacgga tttttgctca
      181 tttacctatt cctggaagta tcttaggtat tcttttttta tttttagcat ttgaaatgca
      241 tcttgttgat cctgaaaaaa tcggagcaac tggtgatttt ttattgaata atctagccat
      301 tttatttgtt cctgctggtg taggtttatt ggaatatttt gatgatattg cttcgatatg
      361 gcctgtttta ctgggagcag tcgtcgtgtg ttccatcgtc acgatggttg cagcgggaaa
      421 aacggcagaa ggtgtagaag ctttgctggg ctatgtccgt aagaaaaaag cagaaagggt
      481 atctgtaaac gaaatggagg agaatcaaat tgactgagtt ttttgtgaat ccattatttg
      541 gcttaagttt gactttagga gtgtacgtat tatctgacaa atttttgcgg aaatataaac
      601 tgcctgtttt gaatcctctc gtattatcga ttgttgttat tatcttattt ttgcattttt
      661 ctgggatcag ctataaagac tataatatcg ggggcaattt tttgactatg ctgattactc
      721 ctgcgacagt ggcacttgcc atccctcttt ataaaaactt ccgtttactg aaagagaatt
      781 tttatcctgt aatggctgcg attttagcag gaatagtcgc aaattgcttg gttagtgtag
      841 ggttcggtta cttcttctct cttcataaag agatggtgat ctcattacta cctaaatctg
      901 taacgacggc tatttctgtc gatttatctc atacgatggg cggggtgaat gccgtgacac
      961 ttgctattgt tgtctctaca ggtatttttg gttcgctgat cgccactcat attttccgcc
     1021 tattcaagat cgaaagtcca gttgctcgcg gtgtggcgtt aggaagtact tctcatgcta
     1081 ttggtacagc aaaagcaatc gagattggtg aaatagaagg aatcatttcc ggacttgcga
     1141 tatgcgtcaa cggaatattg accgttttat tgctaccgtt attctttcag ccttttgctg
     1201 gcttgttttg atgcgaaaac cactatttta tgctacaata accaaaacgt tcgagcataa
     1261 aaaaggaggg aaagacgtgt atttgtcttc cgagtatctt aaacaatttg ataaagatct
     1321 atattataag attctcctcc tagaaagttt tgaggatcaa gcttggcata ccgctgcaca
     1381 attagcgcaa gttgttcagc tggatgcccg cagcgtgtcg aaatacctga acgagctgtc
     1441 taaaaactat cagcagttta gcggtaagac acaccctttg tttacaaaaa atcaccgttc
     1501 tggttataac ttttatgata cgctcgattc aattgaacat gagcgttttt tgatctatct
     1561 cgtgcaaagt acgctgaaat tccagttgct gcacgacatt ttttttgaag aattccatac
     1621 aatgtaccaa tttgcgcaaa agcattacat tagtgaatct actgctcatc gaaaaatcaa
     1681 tgaatggaaa cagcaactgc aaacttacgg aattcggctg caacgaggta catatatcgc
     1741 tcaaggcgaa gaagaaatca ttcgacttta cttgcatatg acattctggc agttgtttcg
     1801 agggaaaatc tggccatttg aaacgatttc tcaaatggac gtaaagaata tggcagaaca
     1861 tatcatggct ttttttaatg ttcgtctgaa cgagatcaaa aaaagacgat tggaatatat
     1921 gctaggtgcc ttttttttaa gaaaatcaca gaagcattat gttgtattga atgagaaaaa
     1981 aagacggctg atttctgaca atctgttgtt ccagcgattt tgccaggtca tggagccggt
     2041 atttcctaat tattttcaag tagaagacga gttaggcgca ttatttcttg tactgatgac
     2101 tcgtgaagaa tattatagtg atcctaagat acggaagaaa atttttgatt ttcatcaaca
     2161 agcaaaaaca ccgcccttta cagccctttc agaagcaaaa gccgctcttt ctctttatca
     2221 ggaagagcag gggctgccag cagaaaatct aaccttcgag gcagaaaact atttattcag
     2281 cagtcatttt ttcgcctatc tttttcctaa tgcgaaagaa acgattgatg gcaatagtag
     2341 tgattttatt aatcatttag ttattgaaaa caaggaattg aaacaatggc tcgtacattt
     2401 ttttgaaagt agacacaaac atcccaatca tctggcattt aaaaatcatg cgtttttgat
     2461 ggggcggtat ctgactgtgt ttaaaacatt aggagccttt actccacagc ttccaaagat
     2521 cacgatcctt ttaatgacag atttcccatt atttgaagag caactgttag aggagggatt
     2581 acggacattt ttccgtaatg agtatcagct gatttttctg ccaacagatt atcgggggag
     2641 agaagttgat cttctgattt cgacaagtaa agtccaccgc aaaccatggg cggatcttga
     2701 ctattttatt gtgactgaag aactaaaatt aattgattac atccaactat ctcaaaaatt
     2761 tgaaatgatt caaaaacaaa aactattaaa acaataacaa agaaggagaa aaaagatgac
     2821 atttgaagaa attttaccag aattgaaaaa aggtgcaaag attattcgta aaggatggag
     2881 tggatttgaa ctttatgttg ttctcgtatc cgatgatgaa tttgatggct gtccggtgac
     2941 gccgtactta ttgatcaaaa caagtgacga aggtttttcc agcttcgctc ctacagtttg
     3001 tgatatttta gcagatgatt gggcgatcgt agaatgaaaa accagtatcc agatctagct
     3061 aaacaggttg tatttatcac aggagctgct tctggaattg gtgctgcgca agcccgagcg
     3121 tttcttgaac aagatgcttt tgtatttgga ttagatcaaa agttcggtaa aatggaagaa
     3181 atttctttgc gatttcctga aacttttgcc tacgcactag gtgatgttcg acaaatgaaa
     3241 gaactccaac aagcggtatc gaaatgccaa aatcattttg gtgaagttac gattttactg
     3301 aatacagcgg ggatattgga tgcctacaaa cccttgatgg aaactgacga atcgctgtgg
     3361 gatctgattt atgagacgaa tgtgaaaagc atgtatcagc tgacaaaaat catccttcct
     3421 gatatgatcc gccaaaaaag cggaacgatc atcaatatgg cttcgattgc tggcttgatt
     3481 gctggtggag gtgggatcgc ttatacaagc gcgaaacatg cgattgtcgg atttaccaaa
     3541 caactcgctc tggattacgc aggacagggt atccgagtaa aaggaattgc tccaggagcc
     3601 atccaaacac caatgaatgc ggctgatttt gaagggaaag gtgagatggc agagtgggtg
     3661 gcaaatgaaa caccagtcaa gcgatgggca cagccggaag aagtagctga gctgacgtta
     3721 tttcttgcct ctcctcaagc aagctacata caaggaacga tcatcccaat cgacggcgga
     3781 tggttgttaa aataaccttg tgtttttttc aagcttctgt atgataaaac taaagagaga
     3841 aacaacggtt gatgataaaa aaagtcgatg actgttcccg acattcgatg ccgaacagcc
     3901 agttcaaaaa attcttttta ataatcgtta atattcgaaa ctaagcggct tttcacgacc
     3961 tcttgatata aacgatattc aaaagcttgc aactgcggta acaagtccag ccaaatgaaa
     4021 aatataagga gctattttta tttgaccatt cttattactt gttgcagggc agctttttca
     4081 ctaccttctg cgacacttca gttggaattt tcttcccatc ctttggtcgc ggtcattacc
     4141 aagataaaat tatcttgcaa aggagaaata gaacatggaa aaaaataata cccgttcagc
     4201 caaacgctgg acaacgacag atgtgactcg gatggcgcta gtcactggtt tatatgtggc
     4261 agtgacgctg gttttgtctg tctttagctt tggagtgatt cagattcgtc tctcggagat
     4321 gttcaactat ctgtccttgt ataacaaaag atacatcgtc gcggtaacag cgggagtagc
     4381 tttggctaat attgcttctc ctcttggact gatagatgtt gcagtaggaa gcgtcagcac
     4441 gctgcttgta ttgcttatca actataaaat cactagtcgc atcaaaaaca tgaaaatcaa
     4501 aatggccgtg acagcatgcg tatttgcttt ttccatgttt acagtagctg gtcagctaac
     4561 gattttatat caactacctt ttttcttgaa ttggtgggtg attgctttag gagaattggc
     4621 atcaatgatt gtcggtggga taattattta ttggatcggt aaaaaagtgg atttgtcaaa
     4681 ataaagatct gtttaattta aaatagggca gcatggaaga gactgaatgt tgagtcttct
     4741 tccatgctgc ttttttgatt aatttgtatg aagaaatcga tttagccgtt ttttcatcaa
     4801 gataccgata taagctgctc cagttgcctt gattgcttcc ggcagtaaaa acggcagtag
     4861 tccactggca aatgcagttg taaaagtaag atcaccactt attttcagcc agatcgttcc
     4921 gcagatcaat gcagcaagag agcccattag attggcaaag attgcatata agtaatgagc
     4981 atccgttctt tcgatcatcc aaccagttat ccacgtttgt aagagaaatc caatcaaata
     5041 tccgccagtt ggcccaaata aaacagctat acctccagtc atccctgaaa atacaggaag
     5101 accaatcaag ccaagtaaaa gataaactcc aacagatatc aatcctagtt ttcttccgag
     5161 aaaagttgct gttaatccaa tagcaaatgt ttgccctgtc aaaggaacca aaccaaaagg
     5221 aatggtcatc tgagaaagga cagaaataat cgcggcaaat tccgcagcaa gtatcaattc
     5281 tttcaatgtt cggttcataa cgccctcttt tcgttaactt aatattttta tttggttaac
     5341 gaaattgtaa aacaaagtga ttactttgta aatagaaaat ataaaaaaag accagctatc
     5401 caatagatag cggtcttttt caagaaagct tttgaaatta taagctttgg ttgtagtatt
     5461 caacgacaag agcttcgtcg atttctggat atagttcgtc acgttctggt aaacgagtca
     5521 atgaaccttc caatttttca gcgtcaaagc ttacgaatgc tgggcgtcca acagttgctt
     5581 ctacagcttc ttttactgtt acgatgtttt gtgatttttc acgaactgag atcacttgac
     5641 ccacttctac gtggtatgaa gggatatcaa cgcgtttgcc atcaactaaa atgtgaccat
     5701 ggttaactaa ttgacgtgct tgacgacgag tcgttgcaag tcctaaacgg taaacaacat
     5761 tgtctaaacg ttgttctagt aagatcatga agttaacacc atgtttacct tctttgattt
     5821 tgctagcttt tacgaacaag ttacggaatt gacgttcgtt cattccgtac atgtgacgaa
     5881 gtttttgttt ttcagttaat tgcatgccgt attctgattg tttgccacgg ctattaggtc
     5941 catgttgacc tggtgcgtaa gggcgacgtg ctaattcttt tcctgtgcct gacaatgaga
     6001 tgcctaagcg acgagaaatt ttccatgatg gtcctgtata acgagacatt aaaatttcct
     6061 ccaatgaata taagtttttg gagtaaaata atccgttgaa aaattcataa tcatatagtt
     6121 tgttcttcaa tcttcacctt tgcagccgcg gttacacaat cgaacccgag acggggcaac
     6181 aaactaggga tgagttatta tttttctgct gcattatttt acacaaatta cagtatatga
     6241 tagatcatcc acgaatgtca agataattag tacataaaaa cgctttacaa acctaggctg
     6301 tctttataaa atgagagggt atgtgcaaat ggaaaaaagc gaaggaagtt taaggagaaa
     6361 atcaatgaat ccggtatcac cattatttga gaaaatcgat gcttcaattg aaaagaaggc
     6421 gaatcttttt gaaaacagct tttcacgtta cgctgttcga gcggttctcg cctgtttgtt
     6481 tttgacttta ggaacggctg tggcatttgc gatcgcgatg aagggggaag atatcgcgca
     6541 tggtctagga aagattttat acgcatttat gttcagctgg ggtctggtca tgattctata
     6601 catgaacgcc gaattaggaa catcgaatat gctttatatg accgtgggtg tttttcggca
     6661 gagacttgct ttaagtaagg cagccaagat tctatttgcc tgtatttttt tcaatatgat
     6721 tggcggtata ttatgtggat ttctaatttc tatgactacg ccatttcaag atttaacgtt
     6781 ggacaacttt atgttagaat caatagcagt aaaactagca aaaccttcag caacgatttt
     6841 ggtggaaggg atgtttgcga atatcgttgt caataccgct gtactgatca gcatgcggat
     6901 gaaagaggat gtcggaaaag tgatcacagt cgtattcatc attttcattt ttgctttttt
     6961 aggatatgaa cacgtgatag cgaattttcc tgcctttacg cttacttact ttgcttctca
     7021 cggacagatg gatggaatga cagtcagcaa tgtgttacat aatttgtttt ttgctttggc
     7081 aggaaattat attggtggcg gtttagtaat gggactggga tatgcttggc tgaatcagtc
     7141 aaaatcatcc tatgtggact aaaaagtcgt tcattaaagg ggaagtgcca tggaagtttt
     7201 agtttttcca aaaaatgagg aaaaagcgtt acaaatggaa aagtacatga aaaatctttt
     7261 ttcctttgct ggagtaccta agccggaacg cgctctttta caacaaccgt tactggatga
     7321 gagcaaaaat ttatctattt ctgaagtttt agcttttgtt cattattatt atgaacaaat
     7381 cgaaagagag tatcagtatg tagcgatcga tttagtccag aaaaacatca aacggcttac
     7441 atttgaagac ttagtcgtat tgttgccact agttcagaaa aaagaatggt gggacagcat
     7501 cgactcttgg agaaaagtat acagcacatg gtgtaaagcc tacccagagc agcgtgaaga
     7561 agtctttcaa ttgtttgaaa aaaaggaaga tttttggcta cgtcgaatag caatcacgtt
     7621 gcaattaggt tataaacagc aaacagacac aggactattg atccgagcaa tagacgaaga
     7681 tcgtcaaaca aaggaattct ttatccaaaa agcaattggt tgggcattac gcgattatag
     7741 taagaccgat gccgattggg taaaaagaca gctgacaaca gatttgagta agttagctgt
     7801 tcgagaggca agtaagtatt tataagaaag cgagacgaca agagatgaga cgcaaattat
     7861 ttgcttttga tattgatgga acgttgttag attcaaacaa acaagcttta gatagcacaa
     7921 gagaagcatt ggaaaaatta cgcaaagccg gtcatctggt cacgattgct acagggcgga
     7981 gccgatttca tgcgcaagag atcattcgcg atcttgattt tacgaactac attttatgta
     8041 atggagcggc cggtttttta gaccatgaac aagtatataa gaatttacta gatcgcgacc
     8101 agttggatgc gttcgttcaa gaagcgcatc atgagcagat cgatacagct tttgtcagct
     8161 tagacaatat caagcgttct tcttccaatg atgtaacggt gatggaagaa gcgatgcatt
     8221 ctttcggtgc tgtcttgcct gaattagatc agttttttgc agaggaacaa gaaatatacc
     8281 aagcgttagc atttttcgat caaacatatg atgggaagtt ctcttcatat gataaaattc
     8341 gatttgtccg ctggcatgag aacagtgtag atgtcgtacc taaaaatggt tctaaagcag
     8401 cgacaattct gcacttagca gaacaagtag gcatccctca tgaagacatc atcagctttg
     8461 gcgatggaca aaacgaccgt gaaatgttgc gcatgtcagg aattgggata gctatgggga
     8521 atgctgtgcc agaagtacaa gcagaagcta agatggtcac tgatacaaac gatcaggatg
     8581 gaatttggaa ggcattgaaa gagttaagcg tgatttaaaa gaggttgtga aaaagctgtt
     8641 ctgcatcaag taataagccc agccaaacaa aaatagctcc tcctattttt cgtttttctg
     8701 ggcttattac cgcagatgca agctttcgaa caccgtttaa ggaagaggtc gtgacagaag
     8761 tggtcagttt caagaaataa gaagggattt acgaaaattg cttctcaaat ttttgtaaaa
     8821 ttacggctta tttctgaaga agtagatgaa acaacgttta ttcgggaaat gggactgtga
     8881 caagatgttt gtcatagtcc cattttttta ttttattttt caaaactaag aaataccagg
     8941 aaagagttcc ttttgtttga ccttttcttt cttattgaaa acaactataa tatagagtga
     9001 tgttaaaaga gaagaggaat ttagtgtgcg ggtattgttt attggtgatg tagtaggttc
     9061 aatgggacga gagatgatta ctgaatatct tcctagattg aagaagaagt accgtccgca
     9121 agtaacgatt gtaaatggtg agaatgccgc ttctggacgt ggaatcacag aaaaaatcta
     9181 taaaaaattt ttgcaagacg gcgtagatgt cgtgacgatg gggaatcata cgtgggacaa
     9241 ccgcggaatc tttgagtttg taaatgaagc taaaaagatg gttcgtccgg caaattttcc
     9301 ggaagggacg cctggacaag ggatggtctt tgtcaaagtg aaccaagtcg aactggcagt
     9361 gatcaacatg caggctcgtt cttttatggt ggacttggat gatccttttc gtaaaatgaa
     9421 tgagctggta gaagaagcca gaaaacgtac gccgatcatt tttgtagatt ttcatggaga
     9481 aacaacaagt gaaaaacaag caatgggctg gtttttagat gggaaagttt cagcagtggt
     9541 cggtacacat acacacgttc agacaaatga tgcgcgaatt ttgccaagag gaacagcgta
     9601 tctgacagac gttgggatga caggtcccta tgatggtatt ttgggtatgc gcagagaacc
     9661 tgtaatcgaa aagtttctga ctgcattacc aaaacgtttt gaagttgtgg aacaaggccg
     9721 atcgatttta agcggctgta tcttggaatt ggatgatacg acaggtcatg caaaagaaat
     9781 ccagctgatt cagatcaacg atgatcgacc gttcatggag tagaaagaga ggcagttttt
     9841 tgcagcagga gccggaaatt caaaaagaag taaggaaact gacaaaactt ttaagagaga
     9901 atgaaacgat cattcgctac aaagaacttg aagaaaagat ccagcaaaac cagtatttag
     9961 ctgagttgag agaaaaaatc aaacaagcgc aaaaagatgc tgttcatttt gcccactatg
    10021 acaaaccagc agcagaaaaa gaagcaatca aacaagcgga tcaattcatg caagaatttg
    10081 atcagcatcc gcttgttgta gcttatagaa aacagctgtt ggaagcagat gatctgcttc
    10141 atcatttaac aactatgata caagaagaaa tcaatggaca aatagaggag gaaaaacatg
    10201 cctcaaaaaa ctaaaaatac tcctatgatg gaacaatatc taagcatcaa ggcccaatac
    10261 caagatgctt ttttgttcta tcgtttagga gacttttatg aactatttta cgaagatgcg
    10321 atcaaagcgt cacagctttt ggaattaacg ttgacgagca gaaatcgaaa tgcagaagaa
    10381 cctattccta tgtgcggtgt acctcaccat gcagcacaag gatatatcga tgccttgatt
    10441 gaaaaaggct ataaagtagc gatttgcgag caggtagaag atccaaagac gactaaaggg
    10501 atggtcaagc gcgaagtgat ccagctagtc actcctggta cagtcatgga cagcaaagga
    10561 ttgtcggcaa aagacaacaa ttttttaaca gctgtagtta gtcaaggaaa tgaatttggc
    10621 tttgcttatg ccgatttaag tacgggagaa ttgaagacgg ctcggttgca tgatgaagaa
    10681 gctgttttaa atgaagcctc tgcattgcag acaaaagaac tcgtattagg cagtgagatc
    10741 actgataccc ttcgtgaaca gctaagcggg cgtttagggc tagtcttttc tcaacaaaat
    10801 gagatggaag aaaacgcgga atttagcttt ttgacaagcg aactagtcga tccgctggaa
    10861 aaagaagcaa ccggcaaact attgacttat ttatcggtga cacaaaagcg gagtttagct
    10921 catatccaaa aagcagtaga ataccaacca gatcattttt tgaaaatgga ttattattca
    10981 aaattcaacc tagaattaag ccaatctatt cggactggga aaaagcacgg cactcttcta
    11041 tggttgttag atgaaacaaa aacagcaatg ggtggccgtt tactaaaaca atggctagac
    11101 cgtccgctta tccaggcaaa acaaattaaa gcaagacaag aaatggtcgc ttctttgcta
    11161 gacagctatt ttgaacgtat cgatttgcag tcagcattaa cgaaagtcta tgatctggaa
    11221 cgcctagcag gtcgagtagc tttcggtaat gtcaatgggc gagacttgat ccagctgaaa
    11281 acttctttag aacaagtgcc aatgatcaga gggttgattg aaggaatcga tcaaggtcaa
    11341 tggcaaagtc tgctttcaga aatgcagccg atggaccatc tcgtccaatt gatcgaccaa
    11401 gctatccaag atgatccgcc gttacaaata acggaaggaa acatcatcaa agatggattc
    11461 aatgagcagc tcgatatgta ccgttctgct atgagaaacg gaaagaaatg gttggcagaa
    11521 cttgaagcaa aagagcgtca agaaacagga atcaagaact taaaaatcgg atttaaccgc
    11581 gttttcggtt attatataga aattacaaaa gcaaatcttg gaaatgcaga actggagaag
    11641 tatgaacgaa aacaaacttt agcaaatgca gagcgtttca tcacaccaga actaaaagaa
    11701 ttggagacac aaatcctcga agcagaagaa aaatctgttg atttagaata tcagttgttt
    11761 ttagctgtga gagaagaagt caaaaaggct atccagccgc tacaagtact tgcaaaagca
    11821 atcagtgcag tagatgtgct acaaagtttt gcaacgatca gcgaacgcta tcagtatgtt
    11881 cggccagaac ttgtcagtga caagcatcag cttctgatca aagacggacg acatcctgta
    11941 gtagaaaaag tattaggaca tcaggaatat atccctaaca gcgtagaaat ggcagaagac
    12001 gaaatgatct tgctgattac aggaccgaat atgtctggta aaagtacgta tatgcgacag
    12061 ttggcattaa ctgttctgat ggcgcaaatg ggctgtttcg ttccggcaaa acaagcagtt
    12121 ttacctattt ttgatcggat ttttacccga attggtgcaa gtgacgactt aattgctgga
    12181 caaagtactt ttatggtaga gatgatggaa gccaatcaag ccctgcgtta tgctacgcca
    12241 aacagtttga ttttgtttga cgaactaggg cgaggaacag caacctatga cggaatggcg
    12301 ttggcccaag caatcatcga atatatccat cgtcatgtac aagcaaaaac actcttttct
    12361 actcattatc atgaactgac ggtacttgag aaagaacttc cgcagcttaa aaatgtccat
    12421 gttggagccg ttgaaaaaga cggagaagtt gtcttcttgc ataaaatgat ggatggacct
    12481 gcggataaaa gctacggcat acatgtggca aagatcgcag gattgccttc caatctacta
    12541 gaacgagcag cagatatcct gcatacgcta gaaaatggcg ataacggaca ccatactgtt
    12601 ccagctgaag tagaagagaa aactaaaaca gccaaacagg agcaaataaa accacctgca
    12661 gaaaaagtaa atcaagacca tatacaagac cgtgtaaaag aagaaaacca acagctttct
    12721 ttattcagtg aattatccga aaatgaaaca gaagtcattg aatcactgaa gcaaataaat
    12781 ttactagaaa tgacgccaat ggatgcacta aacacattat atgaattaca aaaacaactg
    12841 taggaagaag gcggatatcg atggccaaaa tccaagaatt atctgaacgt ttagctaatc
    12901 agatcgctgc gggagaagta gtggagcgac cagcttccgt tgtgaaagaa ttagtggaaa
    12961 atgcaattga cgccggcagt actcagattg atatcttagt tgaagaagcg ggactgcgga
    13021 cgatccaagt gattgataac ggagaaggca ttttggcaga cgacgtagag aatgccttca
    13081 agcggcatgc gacaagtaag atccataatc gcgacgatct ttttcgcata cgaacgctgg
    13141 gctttcgagg ggaagctttg ccaagtattg cctctgtttc tgaaatgact ctggaaacgg
    13201 cgaatcagga agaaaaacaa ggcacctatc tgtcgctgaa aggcggagaa gttattgagc
    13261 atcgaccagc accactgcgt caaggaacga agattaccgt cagcaatctg tttttcaata
    13321 cgccagcccg cttgaaatac gtgaaaacac tgcacacaga attagcgaac atcggggaca
    13381 tcgtcaatcg actggcattg agtcatccat cgattgcttt tcgtttagta catgatggaa
    13441 acaaaatgct ggcaactgct ggaaatggcg atctgaaaca aacaattgcg ggaatctacg
    13501 gattatctac cgcaaaaaaa atgctgaaaa tcgaaggaaa agatctagat tttgagttat
    13561 ttggctatgt ttcattgcct gaggtgactc gtgccagtag gaattactta tcgacgatca
    13621 tcaatggacg ttttatcaag aacttttcct tgaataaagc aatcgttgca ggttatgggt
    13681 caaaattaat ggtcggtcgt ttccctattg ctgtattgga aatcaaaatg gacccgcttt
    13741 tagtagatgt aaatgttcat ccgacgaagc aggaagtccg gctgtcgaag gaaaaagaac
    13801 taacagagct aattagtaac gccattcaaa aagcattgtc tcaagaaaat ctgattccaa
    13861 gtgcagcgga taatctacgg tttaagaaaa aagtacctga tcagcctaaa acagaacaaa
    13921 tcgaaatcga tttgtcctac gcttttgaag aagaaaaacc taaaaagcca agtagtttga
    13981 attttgatcg agagacaggt acctttttcg tagaagaact aaagaacaca tttgttgaca
    14041 gaattgttga taagtctgtg gaaaatgttg aaaactattc tggtttttct gaagaaaaca
    14101 ccacttatcc agaaaaaaca gcgaaaacgg tggataagtt tggtgaaaat gttgataact
    14161 ttgtagaaaa aacagtggaa aaatctgaaa agaaagaatt tcccattgtg gataacatgg
    14221 agaaaacaaa tgaagaacaa gcagataaat ccgtagaaga ggatgaaaag atttacgagg
    14281 aagaattgac actccatcct gaatttgatt tcactcaagg aaacgacaaa acattagaac
    14341 aagccatcaa gaaattagaa acggaaaaac cagtacaacg ttttcctcag ttggaatatt
    14401 ttggacagat gcacggaacg tatctttttg cccaaagtag cgatgggttg tacatcattg
    14461 atcagcatgc tgcccaagag cgcattaagt atgaatattt ccgggaaaaa attggggaag
    14521 tatctgatga tctgcaagaa ttgcttgttc catttgtgtt agactatcca aacagtgatg
    14581 ctttgaaatt aaaagagcaa aaacacttgc tagaagatgc agggatttat ttagaagaat
    14641 ttggtcaaaa cagctttatt gtacgggccc atcctacttg gtatcccaat ggagaagaag
    14701 aatcgattat tcgggagatg atcgatatgc tgctgacaac tggaagtgtg agtgtaaaaa
    14761 aattccgtga agcaacagca atcatgatga gttgcaaacg atcgattaaa gccaaccatt
    14821 atttgaatga agcacaagca cgtgtgctgc tgaaggatct ggcacgatgc gaaaatcctt
    14881 tcaattgtcc ccatggccgt ccagtattga ttcactttac caattcggat atggaaagga
    14941 tgttcaaacg aatccaagac ccgcattaag tcaaagagaa gagggaaaac atgaagattg
    15001 tattagcatc ccaatcacca agaagaaaag agttattggc tcatatcgtt tcagaattcg
    15061 agattgaacc tgctgatatc gatgaaacgc catttccaga cgaacagccc gtcgattatg
    15121 ttcgtagaat ggctgaagaa aaagcacgaa ctgtctggga acaagatgaa aaagaagacc
    15181 atttagtgat tgccagtgac acgactgttg ttctcaatca agagatcatg ggtaaaccag
    15241 aagatctggc tgaggctgaa ttcatgctga aaaaactaag cggggaaaca catactgtat
    15301 atacagctgt tgttttaaaa acaaaggaac gagaagaacg gatactagca gaagcacatg
    15361 tcacttttta tcctttgaca gacgaagaga tcaaacgcta cttggagacg ggggattatg
    15421 cggataaggc aggggcatac ggcatccaaa atgctgcgag tgtattcgtc aaagaaattt
    15481 ctggagatta ttacagtatc gtaggatttc cgattggtgc tgtcaatcag gcactgaaaa
    15541 cattcattta aacaaaaaag cggactatct gattttcgat tgaaaatcca tgatagcccg
    15601 ttttttttat tgaaaagatt acttatctac tctgactaag attttacttc agctttaccg
    15661 actgcttcgc ccgggcgaac aagtcctgat tggtcaagag aaagtcctgc aactttttca
    15721 ttattggtca aagcaacaat aatgtcagtt ttcttgccag cttgttctat ttctggcaaa
    15781 tccatttcag caatcagggt ctctggtgtg acaaggtatc cttctttcac gaatacattg
    15841 aaaggaacac cctttagttc aacagtatct aatcccatgt gaacaagtac ttccagcccc
    15901 tcattagaaa gaataccaat ggcatgtttt gtttcaaaga cactcgtcac tttaccgttt
    15961 actggtgcat agactttgcc gtttgacggt tcgacagcat aaccgtcacc catcattttt
    16021 tgtgagaaaa ctgggtcatt tacttcagtt atttccttga tggtccctgc agcgactgcg
    16081 aataatgttt cttttgttgc aactgccgta ccagttgctg aaacaggttc gaaagactgt
    16141 ggcgttgctg ttgcgccatt tgctgtttga agttgtacat cagcagcatt ttcaggaaca
    16201 gcaggatcaa tcttattcaa aattccctga cgtctaaaga tgatcgtcaa gccaaatgga
    16261 atgatgacag caatgatcat tgcgatgatg aaaggtaccc atgtttctgc ttggatagct
    16321 agtattcctg gcaaaccgcc tactccgatc gcgttagctc gaacacccat caagttagca
    16381 aacatgccag ctaatccaga gccgaccatt gcagcgacga aaggatagac gtatttcaaa
    16441 ttgattccga acatagcagg ttcagttacg cctaagtaac aagaaatcat tgctggaatc
    16501 gagatttgtt cttctttttt attcccacga tgtaagaaga tgatggctaa gactgcagag
    16561 ccttgggcga tatttgataa agcaatcatc ggccataaat tcgttgatcc gaaatctgca
    16621 atcagctgca tatcgatggc attggtcata tggtggagac cagtaatcac taaaggagca
    16681 tacaggaatc cgaatacggc actaaacaac cagctgatag aagacgtcaa tccagtattt
    16741 acgatatttg agatccagct tccgactgtc cagccgatag gacctaaaat aacgtgtgca
    16801 gctaaaacag ttggtaacaa tgaaaacaat ggaacaaaaa tcatcgagat cgactgaggg
    16861 atatatttgc ggaagaagat ttctagatac gctaacataa accctgccag catggctgga
    16921 ataacttgcg cttgataacc aatcatctgg acttgagcaa aaccaaaatc ccaaaatgga
    16981 atatctgctg cggctgtgga agctacacta taagcattta ataattgcgg agaaactaac
    17041 gtaattccta aaacgattcc taatatttgt gtagtaccca tcttttttgc aatactccaa
    17101 gtgataccta ctggaagaaa atggaaaatt gcttcacccg gtaaccacaa aaaggcattg
    17161 acaccattcc aaaacttcga gtgttcaacg attgttccac caagactttc aaactggata
    17221 ccttcaagta catttctgaa tccaaggatc aatccaccga caatgattgc aggaatcagt
    17281 ggtgtaaaga tttctgctaa aacggcaata gcacgttgaa cgggatgaag gttttgtttt
    17341 gcagctgctt ttccttgttc tttggaaacc ccttcaactc cagatactgc aacgaagtcg
    17401 ttataaaaag tagaaacatc gtttcctatg atgacttgga actgtcctgc attcgtgaac
    17461 atccctttta cgctaggtat atcttcgatc gctttttcgt ccgcttttcc tggatcattc
    17521 aggacaaaac gcatacgagt cgcacaatga ctaactgcag caatattttc ttttccgccg
    17581 atatcatgta acagcttctc ggcatctgct tgatattttg ccatatcaat aaaccccttt
    17641 ctcttaactt gtctatgcaa gttgtttctt catttactat actttttttc ggagagaatt
    17701 gcaagcgttt tattaaaaag tacgagagaa aatgatatcg ttttaaaaag cttaaaataa
    17761 gcttttctta aaaataggaa cattgaaata caaaaagagc tgtagtatag tgaagaaatg
    17821 atggaagaac gagtgaggga aaaaacatga ataagttcaa tgaaattttt ttagatttgg
    17881 aacagaaaat tttagcgaaa gaatatcctc cgcattcgct tttacccagt gagaaccaat
    17941 tgatcaaaat ttatggtgtt tcacgagaaa caattagaaa agcgctgaat cttttgacta
    18001 cggaaggtta tattcaaaaa aaacaaggaa aaggttcgat cgtactggat cggaatcgct
    18061 tcgactttcc tatttctgga ttgactagct ataaagagct acaggagacc caacgtattc
    18121 caagtgaaac gatcatccat acgttagaag aaacagaagt gacaaaagca ttgaacgaga
    18181 tcactggctg ggagatcggt gcgcctgttt ggcatttgat acgcgaaaga aaaatcaatg
    18241 gcgaagtagt tatattagac accgattatc tattaaagga aatcgttcct attttaactc
    18301 cagcacaagc acaaggatcg atctatgaat attttgaaaa tgagctttct ttgacgattg
    18361 cttacgcgca aaaggagatc acagtagaag aagtaacgga aatcatgaaa acagcgatgg
    18421 atcttcacga tgacacttat gcagtagtag tcagaagcct cgttcatctg gaagataccc
    18481 gctgtttcga gtattctgag tcggttcacc gattagataa attccgtttc gttgagtttg
    18541 cccgacgccg aaaagtgtaa atagttcaaa agaaagcaaa ttgttggcat ctgtccactt
    18601 tggcgctatg ataggatcac aaagggggca tttttaatgg ataaaccaat aagaccagaa
    18661 aaagaagaac tgaaagaaaa gttatcaccc atggcttatg cagtcacaca agaaaatgca
    18721 acagaacgac catttagcgg gaaatatgat gatttttacg aaaaggggat ctatgtagat
    18781 atcgtcagcg gtgaaccgtt attcagctct gcagaaaaat atgatgcagg ttgtggctgg
    18841 ccatcattca gcaaaccaat aacgaaaaga caagtgcgcg aaaaagccga tttttctcat
    18901 ggcatgcatc gaatagaagt tcgaagcaaa gaagcagatt ctcatctcgg acatgtgttt
    18961 accgatggac cagtagatca aggtggtcta cgttactgca tcaattctgc ctcactgaaa
    19021 ttcattccat atgaaaagat ggatgaatta ggttatgggg aattcaaatc acttgtagaa
    19081 taatggaaat acacaaagag cggtacaata cggggattta ctcgatcgtc cgctcttttt
    19141 ttattcaagc gattgctatt tgaaagaaac gctccctaga ctcctttttt cacaaggtga
    19201 aatgtgctac aataaaggga acgtatgtgt gcaaaagaaa gaggactaga atatgtatga
    19261 atatctgaca ggtgtggtta cctttatcaa cccctattac atagtagtag aaaccaatgg
    19321 tatcggctac cagatcgcac taggaaatcc ttaccgctac agtagcaaac taaatcaaaa
    19381 aataaaaatt tatgtccatc aggtaatacg tgaagatgcg catcttctgt atggatttga
    19441 ttcgctagaa gaaaagcaac tgtttttgcg attagtaagt gtttcaggaa tcggtccgaa
    19501 aagtgccctt gcaatcatgg caagcgaaga ccactccggt ttgattcaag cagtagaatc
    19561 cagtgatgtc acttatttga cgaaatttcc aggagtaggg aaaaagacag cacaacaaat
    19621 gatcttggat ctaaaaggaa aattcggtga gctgagtatc gatacacctt ttagtctatt
    19681 tgatgaagca aacacaaaag acgctacagc tctatctgaa gcaatggaag ctctctcggc
    19741 tcttggttac agtgatcgag aaatcaaacg tgtagaaaaa caattaaaag aagtagataa
    19801 tcagacaacg gatgagtatc tacgccaagc actcaagcta atgatgaaaa aataaggagg
    19861 agaccaaatg acagatgaat acttgttatc accagaatct ggcgaaaatg aactgagttt
    19921 ggaaaaatct cttcgaccac agctgctttc tcaatatatc ggacagagaa aagtcaaaca
    19981 agaacttcag atttacatcg aagctgccag aaatcgggaa gaacctttag atcatgttct
    20041 gttgtacggg cctcctggat taggaaaaac gacgatggcc atggtcattg ctaatgagat
    20101 gaccgtaaat attcgtacga cgagtggccc tgctatcgaa cgtccgggtg acttagtagc
    20161 aatcttgaat gaactggaag caggggatgt cctgtttatc gatgagatcc atcgattacc
    20221 tagagtggta gaagagatgc tttattcagc tatggaagat ttctatgtag atatcatggt
    20281 aggacaaggg ccaaccgcac atccggttca tttccctctt cctcccttta ctttgattgg
    20341 cgcgacgaca cgggcaggta tgctttctgc acctttacga gatcgatttg gcatcatttc
    20401 tcatatggaa tattatgacg aacaggatct gcaggagatc gttttacgtt cttcggatat
    20461 ttttcaaaca gagatcattg aagaaggggc attggaaatt gctcgccgat cacgaggaac
    20521 gcctagaatc gctaatcgct tgctcaaacg ggttcgtgat tttgcgcaag tccaaggaga
    20581 cgggaaaatc gatcgtccta tagcggatca ggcacttact ttgctacagg tcgatcaggc
    20641 tggattggat tatgttgacc agaaattgtt gcgaacaatg atcgagttat atggcggcgg
    20701 accagtaggg ttaagtacgc tttcagtaaa cataggagaa gagcgtgaga cggtagaaga
    20761 tatgtatgaa ccgtatctga tccaaaaagg atttttaaag cgtacaccta gagggcgaat
    20821 tgctactgct tatgcctatg aacattttgg ttacgactat catatatgat ttttgctttt
    20881 agattggagg aaaatgaatg agtgatacaa cacgtacgaa acaattgatc aaaaacgtat
    20941 tgattgattt gtgccaaacg caatcttttc gaaaaatcag cgtccaaaaa atcgcaaacg
    21001 aagcaaaaat caatcgacaa acattttatt atcattttac agataaatac gatttgcttc
    21061 gttgggtcta ttgtgaggat gcattgcatt atttagatcc acaatgtctt tcacttgata
    21121 attgggaaga gcaagcattg aaaatgctga aagcaattgc tgagaagaaa gaattttatg
    21181 caaatactgt gctttctgac ccagaaattt tacaaaaaga gttttctcaa ctgatccaaa
    21241 aatcatttat caagattttt gaacaaatgg atgaagaaag acaactttct gcaagagaca
    21301 aactatttta tgctcgtttt ttttcttacg gttgcagtgg tgtattagtc aattggatca
    21361 cgaatgaata tgaagaaaca ccgatggaaa tcgctatgca gcttttccgt ttagccaagg
    21421 atacggaatt atattcttat cgtctttatg caaaagaaga gttggaagaa cagtaataga
    21481 aaaatttttt gttggaaaag caataatcgc taaaaggaga tggggaaatg acaaaaattt
    21541 tatttgtttg tttgggaaat atttgccgtt caccgatggc agaaggcttg ctgagaaaac
    21601 gaatagcatt agaaggaagg gaagcagaat tttttgtgga ttctgctgct acaagcacgt
    21661 atgaagttgg aaagacaccg caccctggaa cgaaaaaaat attaaaccaa gaaaaagtag
    21721 atatgacgaa tatggttgct cggcaaatca caccccacga ttttgaaact tttgattgga
    21781 tcattggcat ggatcaagaa aatgtagaag aactgaaacg aagagcacca cgctcagcgc
    21841 aggggaaaat ccatttgttt ttaagtagca ttcctggaaa agaaaaagaa aatgttcccg
    21901 acccatacta taccaataac tttgatgaga catatcactt gatcacagaa ggcctgaatc
    21961 attggtggaa tatttggatt gatgaaaata aaataacaaa taatttgtaa agttaacgct
    22021 ttctttttaa ggcgatgtta gagcatttcc ttgttttttt gtaaaatgtt aggtaaaata
    22081 aaattaatta agtaacactt tcttagctaa aggaggagtg cattcgatga acgaagagac
    22141 agtatcgcca atgaagaagc ttacgaagtt agttactcat acttcgtatc aactttttga
    22201 ttcttttgta aatgacactg aagaacatgc taagaatgta cgattgcaag gaaagtttat
    22261 tcttgagctg caaaaagctg ctactgaaca tagtctagtt atcttacaag taagagcaaa
    22321 tcgtttgagt agcaagtatg aaacgatctc aggctgggtg gcgacaaaaa cggtcgaaaa
    22381 agaccgagtg atgatccgcc tacaaaacga agagcagcag atccggatgg ttcctgtaga
    22441 acaaattgaa aaaataacaa tcctttcacc agcaggggaa caggaacggt tatcaaaata
    22501 atttatacca gatagaaaag cacgtatgag cttgtaccat caatcattac taatcaaaaa
    22561 taaccgaatg ctttaagctg atatcagtaa aacggaacgt tttaggatga gtatcatagc
    22621 atgacagcat tcggttgatt agttgaatcg attggatggt acaagttttt ttgataaaaa
    22681 aaggtcctgc tagccgggga gctagcagga taaggagtta aaaatgaaaa agtgttgtta
    22741 gggttgtttg ttggtatgct tatataatag tcattagttg tgaatatttt gtgacgaaag
    22801 ttttcgaa
//
LOCUS       DAKBVG010000018        22225 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000018 DAKBVG010000000
VERSION     DAKBVG010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 22225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22225
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.018"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            60..3287
                     /gene="fss3"
                     /locus_tag="OUD09_000557"
     CDS             60..3287
                     /gene="fss3"
                     /locus_tag="OUD09_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HCT9691856.1"
                     /translation="MYKKQKKKLCVLTILSLAVQSIVGAIAPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCVEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIADYQKKPSFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENTANIDYRVN
                     GNQLIISPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTVKIDVE
                     MEGSLKIKKVDKESGDIIPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTATIKAGETISVTSKNTREKGQIILDKTGIETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGHETQGKAEFKRAEYTL
                     FTVKDGKAVKWTENFKPELLKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDSEKNAVITRDVAAKEQVIRFGFDFFKFAGSTTGTAETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDYTKSDYIFTITEKGQKQPIKTVTVPYGNLTNENFSVSLNR
                     LMLYDLPEEEDSLTSLATWKEGDKELTTLDSTELVDKLSYNLHEIKEDWYIVAQAIDV
                     DATKAAQEKDKKAKPVAIAETTATLANKEKTGTWEILHKLTVEQVLDKTIVLFNYVYE
                     SKEAYEAGDEPVAKDASLNNQAQTVNCMVERHVSIQTKAHLEEGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALFPDGTSKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYDKDGNINGKHNEDLKEKSQTLTPKKVPTTPSTPEQSEMPTVP
                     SGSQESSPTVKAFPQTGEKTSNVLLFIGFSLIFATAGYYFWNRRN"
     gene            3399..3713
                     /locus_tag="OUD09_000558"
     CDS             3399..3713
                     /locus_tag="OUD09_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCT9691857.1"
                     /translation="MELKFVVPDMVETFGKLTYGGEGEVLSEGYGRNTTIVGRSYHLF
                     SNKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLIAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            3726..4100
                     /locus_tag="OUD09_000559"
     CDS             3726..4100
                     /locus_tag="OUD09_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCT9691858.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQLNQGKPTNQNEGKK"
     gene            4101..4454
                     /locus_tag="OUD09_000560"
     CDS             4101..4454
                     /locus_tag="OUD09_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HCT9691859.1"
                     /translation="MKNMEINVEHILDCLDQFGKGELTEEQLTNALTYDEKMFLIMHQ
                     GLLEVGNDAKEDFDVLDWLGEEESFFMVVEVNESLCRQAESVLEEIDVEMPDAIERFL
                     KQLVETKQLPVVVND"
     gene            4523..5539
                     /locus_tag="OUD09_000561"
     CDS             4523..5539
                     /locus_tag="OUD09_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HCT9691860.1"
                     /translation="MYKGHRIRAGDQHLIYHFVLGWLIFLFIGWMSVFYLQELRQFDI
                     SKVSFSTSEIGWSMKDVICLLGSLALSGSMMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQGEGFFKDLNSSWTKESISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LENKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIEEIVAENGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIQALLKSDAELFILDPKNADLADLGTIMPHVYSQK
                     EEISECVEDFYERMIARSRSMKEMPNYKTGENYAYLGLPPNFLIFDEYVAYMGANRFP
                     TSIE"
     gene            6133..8007
                     /gene="ltrA"
                     /locus_tag="OUD09_000562"
     CDS             6133..8007
                     /gene="ltrA"
                     /locus_tag="OUD09_000562"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCT9691861.1"
                     /translation="MNVETDEVQGSGKNVGKSVMEVPRLNTKIRYWEYYDLQETFDQL
                     FTQSRNGKKFYQLYELIISENNILLAYRTIKANKGSSTPGTDSFTIDNYKEMNQAEFI
                     HLILSHLENYKPKSIKRVMIPKPNGEKRPLGIPCMIDRMIQQMFKQILEPICEAKFYE
                     HSYGFRPLRSAKHALGRIMYLINISKMHYAVDIDIKGFFDNVNHRLLIKQLWNIGICD
                     KQVLAILSKSLKSPIQGEGIPSKGTIQGGIISPLLSNVVLNDLDHWVSKQWHTFETKY
                     PYTKGYNKFRALRDTNLKQGYIVRYADDFKIMTNDYPTALKWFHAVKLYLKDRLKLDI
                     SNEKSKIVNLRKCKSEFLGFAICVKQKGKKWVCNSRISNKKKDQIKEEIKQRIKDIQK
                     SPTAQNALLFNALVLGVHNYFRYATHINLDLNRMNYVLSFTTYNRLSNCSTRCYPNKA
                     SPTYKKFYSTTMKTYKVAGVYLYPLCDVSTKTIYGFNTEDTPFTPFGRQRLEHKSLQS
                     LVFQELRKLMESKIPNRTVEYLDSRISRYSMKSGKCEITKQFLPAKAVHCHHYLPKSL
                     GGDDKFDNLRIIHKDIHLLIHTTNKMIIDHYVHELKLVPEQIAKINLYRKMCNLQNIQ
                     "
     gene            8133..8489
                     /locus_tag="OUD09_000563"
     CDS             8133..8489
                     /locus_tag="OUD09_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HCT9691862.1"
                     /translation="MILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRV
                     ALGRMSELGYSMMFGEVDKNFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREIS
                     NIVGLTVHTERENNSV"
     gene            8534..9676
                     /gene="dcm"
                     /locus_tag="OUD09_000564"
     CDS             8534..9676
                     /gene="dcm"
                     /locus_tag="OUD09_000564"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HCT9691863.1"
                     /translation="MTFLDLFAGIGGFRLGMEQAGHQCIGFCEIDEFARRSYKAIHDT
                     RKEVEMHDITSVSDEFVRSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     ATLLQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQSRERI
                     FLIGHSRDACTEQVFPIIGSSSTSDQNIRNLVNINPSNRGMGGQVYGSDSVAPTLTGD
                     EGIKIALPVKDGISVAGMLSGNFEQGNRIYEVTGTAPTLSTKQGGTKIMIRKNETEYQ
                     EVKPGDSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKGVKAYDL"
     gene            9666..9955
                     /locus_tag="OUD09_000565"
                     /pseudo
     CDS             9666..9955
                     /locus_tag="OUD09_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10211..11395
                     /locus_tag="OUD09_000566"
     CDS             10211..11395
                     /locus_tag="OUD09_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HCT9691864.1"
                     /translation="MAQRNLDYRLLKERRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     IASTKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDVRKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYYAIQDLL
                     KGRSIESTTFSIINRYLRFADKVDGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     IV"
     gene            11392..11505
                     /locus_tag="OUD09_000567"
     CDS             11392..11505
                     /locus_tag="OUD09_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HCT9691865.1"
                     /translation="MKRGILMEIVKDMLLVLGGSMLGVTVMCLMNASAAAD"
     gene            12275..14185
                     /gene="ltrA"
                     /locus_tag="OUD09_000568"
     CDS             12275..14185
                     /gene="ltrA"
                     /locus_tag="OUD09_000568"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCT9691866.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            14289..14513
                     /locus_tag="OUD09_000569"
     CDS             14289..14513
                     /locus_tag="OUD09_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9691867.1"
                     /translation="MSGTYLSIYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFL
                     IIAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            14630..15127
                     /locus_tag="OUD09_000570"
     CDS             14630..15127
                     /locus_tag="OUD09_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HCT9691868.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSISELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMSDVARYYIEETGALGEVPASLQNYIDYESYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            15214..15606
                     /locus_tag="OUD09_000571"
     CDS             15214..15606
                     /locus_tag="OUD09_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCT9691869.1"
                     /translation="MKKIRSYTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAV
                     MILGNLPPLSMIEGAFLKYFGIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRRKLTY
                     AGKKVTLGRNQPQEAITAVRSEFYGISN"
     gene            15590..18037
                     /locus_tag="OUD09_000572"
     CDS             15590..18037
                     /locus_tag="OUD09_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCT9691870.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSILSAQERSKNEVTGKLKTVAYDKIDQQTDALISM
                     IGENQVDYRFFIGFKLLLNDQEFSMKSLTVEAKNAFTDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYPLSKKKLEH
                     ETLIKTYDLIKPTRCLVEEKQRYLKIQQEDETAYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVNLTSDEKNKGLLDPYVIMKNTKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSDGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            18040..20223
                     /locus_tag="OUD09_000573"
     CDS             18040..20223
                     /locus_tag="OUD09_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCT9691871.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            20220..21221
                     /locus_tag="OUD09_000574"
     CDS             20220..21221
                     /locus_tag="OUD09_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HCT9691872.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            21236..22150
                     /locus_tag="OUD09_000575"
     CDS             21236..22150
                     /locus_tag="OUD09_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCT9691873.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
BASE COUNT         7410 a         3938 c         4827 g         6050 t
ORIGIN      
        1 caaagatgtt caagcgagca tcttattttt ttgtgcctaa tttgaaagga gaacatttca
       61 tgtataaaaa acaaaaaaag aaattatgtg tattaactat tttgagtttg gctgttcaat
      121 caattgtagg agcaattgct cctacgattg cttttgctga tgaaattacc catccacaaa
      181 cggtaaccgt agaactagat ttagcacatc aatatgctgt tgaaggaact tttagtgatg
      241 gtcgtccaat gtcagaagtc actgttccgc attatgcagt gtacaacggc gttaaacaag
      301 atattttctg tgttgaacca ggagtcccga tttacaatga gttcactcct ggatatgaga
      361 agaacccgtt accagatatg tccgataaag ccaaattagt ttccgtttta tggaaaaaag
      421 taggtacaga tgcagatact catattgtgg cacagaagat gatctggcaa gaagttaatg
      481 ggtataccct tcactcaata aaacgatcag atggtagtgc cgtaaatatc gcagccattg
      541 aggcaaaaat caatcaagcc attgccgatt atcagaagaa accaagcttc cataatagta
      601 cggctaaaac agtattaggg caatcgacta ctgtgacgga tacgaataat ctgaatttgt
      661 cagagttcga tgaggttgtg gaaaataccg caaacattga ttatcgtgtg aatggcaatc
      721 agttgataat cagcccaaat gccaattcaa aagaaagtgg tgtgttgacg cttaagaaat
      781 ctgctggtac cggaacacct gtcgcctata aaaaggctgg ccaacagact ttaatggccg
      841 gggcgatcga taagccgaac acttatacgg tcaaaattga tgtggaaatg gagggttctt
      901 tgaagattaa aaaagttgat aaagaatcgg gtgatattat accaggaacg gttttccatt
      961 tagactttgg aaaaacttta cccgcaaaag acgtgaccac cgataaagaa ggcattgcga
     1021 ccttagatga gattcctcat ggtacaaagg taaccattac tgaaaaatcg gtgccggcgc
     1081 cttatacgat tgataccaca cccatgactg ccacaattaa agcaggtgaa accatttcag
     1141 tgacttcaaa aaatacgcga gaaaaaggtc aaattattct ggataaaacc ggaattgaaa
     1201 cagggagtga tctttggaac gataattact ctttagcggg caatacgttt gccattcgca
     1261 aggatagccc aactggtgaa atcgtccaag aaatgaccac ggatgaaaac ggtcatgcgg
     1321 aaacaccaaa agagattgcc aatgcgttgg aattgggaac ctactatgtg actgaaacca
     1381 aggctagtca tggctttgtg aatactttca aaccagtaaa agtcgaattg aattatgtca
     1441 atcaaacagt ggctcttgtt accagtaacg taaaagggca aaaccaagaa attactggtg
     1501 aaaccacttt gacaaaagaa gacaaagata ccggtcatga aactcaaggg aaagctgagt
     1561 ttaaaagagc tgaatacacc ttgtttactg ttaaggatgg aaaagcggtc aaatggaccg
     1621 agaactttaa accagaattg ttgaagggaa cgaaagcttc tgatgaaacc gtaactttga
     1681 ctttagatga aaagaatcaa gttgccgtta aacacttagc gattaatgag tattactggc
     1741 aagaaaccaa agcgcctgaa ggatatacct tggatgaaac aaagtatcct gtatccatca
     1801 aaaaggtgga tgatagcgaa aaaaatgctg tgattactcg agatgttgcg gcaaaagaac
     1861 aagtgattcg ctttggcttt gatttcttta aatttgctgg atcaactact ggcactgccg
     1921 aaactggctt caatgatcta acctttaaag tgtcgccatt ggaaggaacc aatgaaatca
     1981 ccggtgccga agatgaagcg accacagctt ataacgagca attagggttt gatggttatg
     2041 gcaagtttga aaatcttcct tatggggatt atttacttga agaagtagaa gccccagaag
     2101 gttttcaaaa gattacgcca ttagaaatcc gttctacttt taaagaaaat aaagaggatt
     2161 ataccaagag tgattatatc tttactatta ccgaaaaagg gcaaaagcaa ccgattaaaa
     2221 cggtaacggt cccttatggg aatctaacca atgagaattt ctcagtcagt ttgaaccgtt
     2281 tgatgcttta tgatttgcct gaggaagaag atagcttaac ttctcttgcg acttggaaag
     2341 aaggagataa agaattgact actcttgatt ctaccgagct agtcgataaa ttgagctata
     2401 atctacatga aatcaaagaa gactggtata tcgtagctca agccatcgat gtagatgcta
     2461 caaaagctgc ccaagaaaaa gataaaaaag ccaaaccggt ggcgattgcg gaaacaaccg
     2521 caactttagc gaataaagag aaaacaggaa cttgggagat tctgcataaa ttaactgtcg
     2581 aacaagtttt agacaagacc attgtcttat tcaactatgt gtatgaaagc aaagaagcct
     2641 atgaagctgg tgatgagccg gtagcgaagg atgctagctt gaacaatcaa gcgcaaactg
     2701 ttaattgtat ggtggaacgt catgtttcca tccaaaccaa agcgcactta gaagaaggtt
     2761 ctcagacctt tactcatggt gacgtggtag atatgtttga tgatgtatcc attacccatg
     2821 atgtactaga tggctcaaaa gaagcttttg aaacaattct gtacgcttta tttccagatg
     2881 gtacaagtaa agaaatttgg aaatctggca agattgatta cgaagtgaat gacaaagaat
     2941 ttaccaaaac cgtacttgcg gaaaaagtgg atacaagtaa atatccagaa ggtacaagtt
     3001 ttaccttcaa agaaattaac tatgacaaag atgggaatat caatggaaaa cacaatgaag
     3061 atttgaaaga aaaatctcaa actttaacgc ccaaaaaagt tccgacaaca ccaagtacac
     3121 cagaacaatc tgaaatgcca actgttccaa gtggctctca agaatctagt cctacagtga
     3181 aggcattccc tcaaaccggg gagaaaactt ccaatgtgct actgttcatt ggctttagct
     3241 tgatctttgc gacggctggc tattattttt ggaatcgtcg gaactaaggt gataagatga
     3301 aacatgtaaa tagtagaaaa caatacgcgc gaccgcctcc taaaaaagtg gtcgcgctat
     3361 ttttgtattc aaaactaaca atgaaaagag gaaaaaatat ggaattgaaa tttgttgtac
     3421 cagatatggt cgagacgttc ggaaaactaa catacggtgg tgaaggtgaa gttctatcgg
     3481 aagggtatgg tcgaaatact acaattgttg gtcgaagtta ccatctgttt tcaaataaac
     3541 aacgggccga tgatattgaa gtggtggtag ctgcggaagc cggcgaaaag gattttgacc
     3601 aagatcaacc actaaaagct gtaaatcccc acttaattgc caaaggctat gaaattgaga
     3661 atcgtgggtt taccgattac gtgttgtatg ttgatgattt agtgaaagca taggaggaaa
     3721 agaagatgag attaacagaa ggtattgtcg tagattcagg attaaccttt ggcaagttgc
     3781 gtttttccgc attacgtcga gaagtacgca agcaaaatga ggacggaacg gttagtaacg
     3841 aggtaaaaga acggacctat aatttgaaat cctccgcgca aggtcgaatg atccaagtca
     3901 gtattccagc aaatgtaccc ttacgcgagt ttgcttatga tgcagaggta gagctggtca
     3961 atccaatcgt ggatacggtc gccaattatg tttttcggga aggaaccacc gttaattggt
     4021 ttatcaaggc agatgattta gtcttgaaac gacaactgaa tcaaggaaaa cctacgaacc
     4081 aaaatgaagg aaagaaatag gtgaagaata tggaaattaa tgttgaacat attttagatt
     4141 gtctcgatca atttggcaaa ggtgagctaa cggaggaaca actcactaac gcattaacct
     4201 atgatgaaaa aatgtttctg attatgcatc aagggttact ggaagtaggt aacgatgcca
     4261 aagaagactt tgatgtactg gattggttag gggaagaaga gtctttcttt atggttgtcg
     4321 aggtaaatga aagtttatgt cggcaggcag aatctgtttt ggaagaaatc gatgtggaga
     4381 tgccggatgc catcgaaaga tttttaaaac aattagtgga aacgaaacaa cttcccgtag
     4441 tagtaaatga ctagcataac tttttgaaat tgcccaatga aatcctattg ggctttttcg
     4501 actggaggtg gaaaccatca aaatgtacaa aggacatcgc atacgcgcag gagatcaaca
     4561 cttgatttat cactttgttc ttggctggct aatctttttg ttcattggtt ggatgagcgt
     4621 cttttattta caagaactta gacaatttga tatttctaag gtatcatttt ccactagtga
     4681 aattggctgg tctatgaagg atgtaatctg cttgctagga agtctcgctc tttcaggaag
     4741 catgatgtta ctttatatac acttttttca ggatcattgg cgaagtcttt ggcatcgaca
     4801 aaagttagct cggatgattc tagaaaacca ttggtatgaa gtaaagcaga ctcaaggtga
     4861 aggttttttc aaagatttga atagtagttg gaccaaagag agtatcagtt acttccccaa
     4921 aatttattat cgaatgaaag atggcttact ttctattcgc gttcaaattt cactgggaaa
     4981 atatcaggat cagctattga agttggaaaa taagttagaa agcggactgt attgtgagtt
     5041 agtagaaaaa gaactcaaag actcctatgt ggaatacacc ttgttatacg atatgattgc
     5101 caatcgaatt gggatagaag aaatagtggc agaaaatggg gcgttgcgat tgatgaaaaa
     5161 tcaagtatgg gcgtatgatt ccttgccgca tatgttaatt gctggcggca caggtggtgg
     5221 gaagacctat ttcttactca ccattattca agcactattg aagtcagatg cagaattgtt
     5281 tatcctcgat cccaaaaatg cggacctcgc cgatttaggt acgataatgc ctcacgtata
     5341 ctcccaaaaa gaagaaattt ctgaatgtgt ggaagatttt tacgaacgca tgatagctcg
     5401 tagtaggtca atgaaagaaa tgcccaacta caaaacagga gagaattatg cgtatctcgg
     5461 acttccgccc aactttttaa tctttgatga atacgtggct tatatgggtg cgaatcgttt
     5521 tcctacaagc atcgaataga tgtgaaatgg aagcctattg agattatcaa tagtgacaac
     5581 taatgttttg agagatggaa tgaaagcctt caagttgagc tgaaacatcc ccgctaatac
     5641 tcctacatgc tctaatcaat gaagtagatt ggttttggta tgaagctagg tgaagtcgtc
     5701 tgaaatctgc ctaagtgctt gagcataagg ctgactatag ggcgggcggc tgaaaaagat
     5761 aactatggta agaatgtctg aaaatgactg acgaatctgc gaatgtacgg gtctaaaagg
     5821 ctgatcttat tgaaagatag gaacatacgg acgtatggtg agataccgac aagtaaggtt
     5881 ccatgatatg aaaatcggga tagctaacga agaagctata atttcacagg ctcatagtag
     5941 atacctaagg ttatacatga tagctaaaaa cattggaacg atgtaagcag aaaactatca
     6001 attaaatgac tacttctaga taggtgcata taagtaataa cgaaattgct ttgtttctgt
     6061 gaaagtaagg gcacagtacc gatgaagcgt ctaacaaatg tggagggata gccctaagtc
     6121 tatttaataa caatgaatgt tgaaacagat gaagttcaag gatctggtaa gaacgtggga
     6181 aaatccgtta tggaggtgcc acgcttgaac acaaaaatac gttactggga atattatgat
     6241 ctacaagaaa cattcgatca gctatttact caaagtagaa acgggaaaaa gttttatcaa
     6301 ttatatgagt taatcatttc tgaaaataat attcttttag cataccgtac tatcaaggca
     6361 aacaaggggt cttcgacacc aggaacggat tccttcacaa ttgataacta taaggaaatg
     6421 aaccaagcag agtttatcca tcttattctc agtcatttag aaaattacaa accaaaatcc
     6481 ataaagcgag taatgatacc taaacccaat ggtgagaaac gcccattagg aattccatgc
     6541 atgattgatc gaatgatcca acagatgttc aagcagatac tggagccaat atgtgaagct
     6601 aagttttatg aacatagcta cggtttcaga ccattgagaa gtgcaaagca tgctctaggt
     6661 cgcattatgt atctcataaa catcagtaaa atgcactatg cagtagacat tgatatcaaa
     6721 ggtttctttg ataatgtaaa ccaccgttta ctgataaaac aattatggaa tataggaatt
     6781 tgtgacaaac aagtcttagc tattctttca aaatctctga aatcacccat tcaaggagaa
     6841 ggtatcccga gcaaaggaac gatccaagga ggtatcattt ctccactatt atctaatgtt
     6901 gttctaaacg acttagacca ttgggtctca aagcagtggc atacctttga aacaaagtat
     6961 ccctatacta aaggttacaa taaatttcgt gctctaagag atacaaatct aaagcaaggc
     7021 tatattgtca ggtacgcaga tgactttaaa atcatgacta atgattatcc gacagcatta
     7081 aaatggttcc acgctgtcaa gctttaccta aaagacagac ttaaattaga tatttccaat
     7141 gagaagtcta aaatcgtcaa tttgcgtaaa tgtaagtcgg aatttttagg attcgctatt
     7201 tgcgtgaaac aaaaagggaa aaaatgggtt tgtaattcac gtatttctaa caaaaaaaag
     7261 gatcagatta aagaagagat taaacaaaga atcaaagata ttcaaaagag tcctacagca
     7321 caaaatgctc tcctgtttaa tgctctcgtg ctcggtgttc ataattattt caggtatgcc
     7381 actcatatta atttagattt gaataggatg aactacgttc tttcattcac tacttacaac
     7441 cgattatcaa attgtagtac aagatgttac cccaacaaag cttccccaac ttacaaaaag
     7501 ttttactcaa caacgatgaa aacgtataaa gtagctggag tctatttgta ccctctttgc
     7561 gacgtatcta caaaaacaat ttacggattt aatacggagg atacaccgtt tactccgttt
     7621 ggtcgtcaaa gattggagca caaaagccta cagtctcttg tgttccaaga attaagaaaa
     7681 ttgatggaat cgaaaatacc gaatagaacg gttgagtatt tagatagtag aatatctcgt
     7741 tactcaatga aatcaggcaa gtgtgagatt acaaaacaat ttttgccagc aaaagccgtt
     7801 cactgtcatc actatttgcc aaaaagtctt ggtggagacg acaaattcga taacttgaga
     7861 attattcata aggatatcca tcttctgatc catacaacaa ataaaatgat aatcgatcac
     7921 tacgtgcatg aactgaaact ggtgccagag caaatagcaa aaatcaacct ttatcgaaag
     7981 atgtgtaatc tgcaaaacat tcaatagaaa ataaatagat ggaacgccgt atgaggtgaa
     8041 agtctcacgt acggtgtgga gcgggggaaa agatggagat tatatcaaat tcttacctat
     8101 cgctatagat gttaacgaca aaagaaagtg cggtgatttt gaataagctt aaacaaatcg
     8161 tgatgttagg ccgtcaatct ggtttctttc tgattctagc ttgtcaaagg ccggatgcga
     8221 aatatctagg agatgggatt cgcgatcagt ttaacttccg tgtggctttg ggtcgtatga
     8281 gtgagcttgg gtattccatg atgtttggag aagtcgataa aaattttttt atgaaacgca
     8341 tcaaaggacg aggttatgta gatacagggg gcagtgtgat tagtgagttt tatacgccac
     8401 ttgtaccaaa agggtatgat ttcttaaggg aaattagcaa cattgttgga ttaaccgttc
     8461 atacggaaag agaaaataat agtgtgtagc ctaggaatat caataatcct gggctttttt
     8521 attgaggtga tttatgacat ttttagattt attcgccggg attggtggct ttcgcttagg
     8581 tatggaacaa gccggtcacc agtgtatcgg tttttgtgaa attgatgaat ttgctcggag
     8641 gagttataaa gcaatccatg atacaaggaa ggaggtggaa atgcatgaca tcacaagtgt
     8701 atcagacgaa tttgttcgat ccctcggacc agtggacatc ctttgcggcg gctttccgtg
     8761 ccaagctttt tcaattgcgg gaaagcggca aggattttcc gatactcgag gtactttatt
     8821 ctttgaaatc gctcggttcg ccactcttct ccaacctaag tttctattcc ttgagaacgt
     8881 ccggggattg ctcaatcacg aaggaggggc tacgttcgag acgatcctcc gaacgttgga
     8941 tggattgggg tatgatgtgg aatggcaagt gcttaactca aaggcctacg ttccccaaag
     9001 ccgtgagcgg atcttcctta tcggacattc tcgagatgca tgtaccgaac aagtatttcc
     9061 tatcattgga tcgtcttcaa catctgatca aaacatcaga aacttggtaa atatcaatcc
     9121 ttccaatcga ggaatgggtg gccaagtcta tggttcagat agcgtagcac caaccttgac
     9181 cggtgatgaa ggaattaaaa ttgccttacc ggtaaaggat gggatttctg tagcgggtat
     9241 gttgtcggga aattttgaac aaggcaatag gatttatgag gtaaccggaa cagcccccac
     9301 tttgtcaacg aaacaagggg gaacgaagat tatgattcgg aaaaatgaaa cagaatatca
     9361 agaggtgaag ccaggggaca gtgtgaacct ggcttttcct aattccacaa gtcgccgagg
     9421 tcgtctggga aaacaaagtg tgcatacctt actaacagga gaccaacaag cggtagtcac
     9481 ggatcagtac cagattcgaa agttaacacc tagggagtgc tggcggcttc agggctttcc
     9541 agattgggct tttgatcgag cttctcaagt caattcagat agtcagttgt ataaacaggc
     9601 cggaaactca gtaacagttc cggtcatttt tgatattgct agaagattaa agggggtaaa
     9661 agcatatgat ttatgacgag ttgattggag aaatctactg ggtgattggc aagattcagg
     9721 cagatccgaa attggaagaa gaactgtacc ggctcaattt tgatattcgc aaaaacgggg
     9781 tgaaagtcca ggtgattcgt atgtagtgga tgaggaaacg gatgctcgaa tggagctcaa
     9841 tcaagtcatc ggtgaattgg aacgaattgc ggaccttgcc aaagaacctg atatccgcca
     9901 atacttgttt gagataaaag cagagttgga aattgagggt atcaccgcgg agtaataggg
     9961 aaataaccca ctgataggca ctgaccgcgg gggatttgcg aagcagaaat cctccgtggt
    10021 gctgtgcctg ttcatggacg gcggcagccg gccatgatgt ctcacccccc gtatctaaca
    10081 ggggggtaca aaaacacaaa aaacaagcaa gtaaccataa aaaatgcttg ttgtatcaag
    10141 gatattaagt aatttgtata ggtgttaact catttggaaa agttaacacc ttgaatttgg
    10201 aggtgaaaac ttggcgcaac gaaatttaga ttatcggtta ttaaaagaac gacgcaacga
    10261 atatggcgtt tcacaaaata aattagctac tgcttgcgga cttagccgcc catacctaaa
    10321 tcagatagaa aacggtggcg taatcgcctc tacaaagacc atgagaaaaa tttttgatca
    10381 actggaaagt ttcaatcccg atttaccttt aacgttacta tttgactatg taaggattcg
    10441 ctttcccaca acggatgtgc ggaaaattat tcaagaaatt ctccatttga aattcgagta
    10501 tatgcttcat gaagattacg ccttttactc ctatcaagaa caatatgtca tgggcgatat
    10561 tgtagtgatg ttatctcatg aagaagataa aggtgtcctt ttggaattaa aaggtcgtgg
    10621 ttgccggcag tttgaaactt ttttactcgc ccaaaagcgt agctggtacg acttttttga
    10681 agattgtcta aaagccggtg gcgtgatgaa acgcttggat ttagcaatca atgatcgagt
    10741 ggggctactg gatattcctg atttaacgaa aaaatgtcag aaggaagaat gtatctcctt
    10801 gtttcgtacc ttcaaaagtt accgttccgg ggaactgttg aaagctgatg aaaaggatgg
    10861 tatgggcaat accttatata ttggcagcct caaaagcgag gtttattttt gtttgtacga
    10921 aaaagattat gaacaatata tcaagttagg gattccattg gaccagaccg aaacgaaaaa
    10981 tcgttttgag attcggttga aaaatgaccg tgcttattat gcgattcaag atttgttaaa
    11041 aggtcgtagc attgaaagta ccacgttttc cattatcaat cggtatttgc gatttgcgga
    11101 taaagtagat ggaaaacggc gaaccaattg gcccttgaat gaacaatggg gtcgttttat
    11161 tggacggaat cggaaagaga ttcaattaac ttctgaaccg aaaccgtata ccattgaacg
    11221 aacattaaat tggctaggta gacaagtcgc ccctacgtgg aaaatggcta aggagttgga
    11281 tcgattgaat caaacaacgt atatccagga tatggtacag aatgcgcgac tctcggaccg
    11341 gcataaaaag attttggagc aacaaagtat ggcaatcgaa aacttgattg tatgaaaaga
    11401 ggaattttga tggaaattgt aaaagatatg ttattggtac ttggtggcag tatgcttggt
    11461 gttaccgtga tgtgcttgat gaatgcaagt gcagcagcgg attgagcaat ggaaaagaaa
    11521 ggaaagtgac aaaatgaatt tcggacaaaa tttatgtgcg actcgttcct tagtgaaaag
    11581 ctaaggcact aataaaagaa cgaaaaagtt tggttattag gagaactgat aatctgacag
    11641 gtggaatgat gaagtaacgc tctgaaacgc ctgccctgag cctccgacaa gcattgtatg
    11701 aggaaactca gaatgtttga agctcggtaa agacggctga aagataccgt aatagttgat
    11761 gaacatatat cttctatcga aaactatcca gaggtgggta gcgtatccaa caggtcgggg
    11821 gtctataaaa tatctatggt tagaatgttc tataaggaac tgacgaaaca gcgaatgtac
    11881 gggtctaaat ttgaactttg tagaaatgca aaggtacgag aaatcgtagc tggtgtggca
    11941 aagtaagaat ataggttatg aaatgccata taacgttaca ggcggtatca agccagcagg
    12001 cttatagcta gtgacctaag gatatatatg tatagataag attatcggaa cgaggaaagg
    12061 tattgggttc tcatcaagag aataaactga cgaagaacaa taagcagttg aactaatgct
    12121 gaaaagcaga gggcgaacct aagaaattct tgtaatgaga atggaggaat gccctcaagt
    12181 cgtgtatata ttagaaaagt attatgttgt ttcaataagg attacgagta agataaaaaa
    12241 gctcactcaa tttcaagtaa aggagtgaag ccttatgcct aaaaataaaa atgaaacatt
    12301 gtgtgtagaa gatttaagac acgctgaata ttacgaaatg caaaacacct ttgatgattt
    12361 atatgccaaa agtaagaatg gagatatttt cactcatctt atggatatta tcctatcgag
    12421 agaaaatata ttactggcat atcgaaatat caaagcaaat gcaggcagta aaacagcagg
    12481 aacagacggt acaataatta aagatattgg caaattgcca gcagaaacag ttgtcaaaaa
    12541 agtaagatac attgttgcag gcagtccaca tgggtatcga cctaagccag tgaggcgaaa
    12601 agaaatacca aaaccaaatg gtaaaaccag acctctaggt attccttgta tgtgggacag
    12661 gcttatacaa caatgtataa aacaagtatt agaaccaatc tgcgaagcta aatttagtga
    12721 aaacagctat ggttttagac ctaataggtc agttgaaaat gcaataaaag caacctataa
    12781 ccgcctacaa atcagccagc tacattatgt gattgaattt gatattaagg gtttctttga
    12841 caacgtgaac cattccaagc tgataaaaca gatatgggct atgggaatac gggacaagca
    12901 cttgatattc attttaaaaa ggatattaaa agctccaatc aaaatgacta acggtacaat
    12961 aacatatcca gagaaaggaa ctccgcaagg gggaataata tccccactac tagcaaacat
    13021 tgttcttaat gagttagacc attgggtaga aagccaatgg caggaaaatc ctgttacaaa
    13081 aaactatgtg gttcatatca ataaaagcgg tagcccatgt aaaagcaacg cttacaaaga
    13141 aatgaagaaa acaaagttaa aggaaatgta tatggttcgt tatgcagatg attttagagt
    13201 tttctgccgc tacaaggaaa gtgctgaaaa agccaaaata gcaatcactc aatggattga
    13261 acaacgatta aaattggaag tatcacaaga aaaaacaaga attgtaaatg ttagaaaaag
    13321 atactccgac tttcttggtt tcaagattaa aatgataccg agaagaaaga agttagttgt
    13381 taaatcacat ataagtgata aacaattcaa aaaccaaaaa gacaagctgg tatcacaggc
    13441 aaagaaaatt gcttctccct caaaagggaa aacggaatta gaagaaacaa gactttataa
    13501 ttcaatggta ttaggtatgc aaaattatta tcagattgca acgaatgtaa atctggattg
    13561 tatgaaactg aatagagctg taatggctat cttcactaac agattaggta caaacaaaag
    13621 gggcagattg agaaaaacag gcagacaact tacgacaaag gaggcaacaa gatatggtaa
    13681 atctaaaatg cttcggtatg ttgctggcga gggcgaacct attttccctg ttggatatat
    13741 ccaacacaga aaaccaatgg caaaggtgta tttagcgaat tgctacactc tagagggaag
    13801 aaaatttatc cacaccaatc taagtgttga taaattactg atgtaccagt taatgaaatt
    13861 atctttggaa aatcgcacaa tagaatacgc agacaatcga atttcgctat tttctgctca
    13921 acatggaaaa tgtgctataa ctctggaaga atttcaacaa gctaatgaaa tacattgtca
    13981 tcatattgta ccaactggag ctggtggaaa tgacgattat aaaaatctta ttttggtaaa
    14041 agaggcggta caccgtctaa tacacgctaa aacagaagaa acaatacaaa aatatatagc
    14101 tctattgaag ctaaataaag tccagctcat aagactaaat aaatatagag tattagctgg
    14161 caatcaagaa ttaaacttga tataggtcta ctacataaat acttttcaat ataaacacga
    14221 tggaacgcgg agtgcggtga aagtcgcacg ctccgtgtga atggggggaa aagtttgaga
    14281 tatttataat atcaggaact tacctatcca tatataactg gtttctatca aacgctcaat
    14341 cactggtgct tttagcaatc gttgtgattg gcttgtatct tggcttcaag cgtgagttta
    14401 gcaaactgat tggcttttta attattgcga ttattgcggt tggcttagtc ttcaacgctg
    14461 ctggagtaaa agacatttta ctagagctat tcaatcgcat tattggtgct taaataaaac
    14521 cgttcttttg tggaatataa gtggttttct tatgttccgc aaaggaatgg tacaccaaac
    14581 gaagtgcggt agggattttt gaatctctac aaagaaagga cgtgaatata tggacgatat
    14641 gcaagtctat attgcgaatt taggcaaata caatgagggc gaattggtcg gtgcgtggtt
    14701 tacctttccc attgactttg aggaagtcaa agagaaaatc ggcttgaatg atgaatatga
    14761 ggaatacgcc attcatgatt acgagttacc ctttacggtt gacgaataca cttctattag
    14821 cgaactcaat cgcctatggg aaatggtatc ggagttgcca gaagaactac aatcggagct
    14881 atctgctctg ctcactcatt tttcaagtat tgaagaacta agcgaacatc aagaagatat
    14941 tatcattcat tccgattgtg atgatatgtc tgacgtggca cgctactaca ttgaagaaac
    15001 gggtgcttta ggcgaagtgc cagccagtct tcaaaactat attgattatg aatcctatgg
    15061 tcgggattta gacctttcgg gaacgtttat ctcaaccaat cacgggattt ttgaaatcgt
    15121 ctattaaatc tgtcggtaca ttcttactgg cagattttct tgtttacggg gtagtcaaaa
    15181 gactatcccc atttttatga aaggattgat tacatgaaga aaatacgaag ctataccagt
    15241 atctggtctg ttgagaaagt gttgtactcc atcaatgact ttagacttcc gtttcccata
    15301 acctttacgc aaatgacatg gtttgtcgtg tcactctttg cagttatgat acttggcaac
    15361 ttgccccctc tttctatgat agagggagca tttctcaaat actttgggat tcctgtggct
    15421 ttcacatggt ttatgtctac aaaaaccttt gatggtaaaa agccttatgg atttttgaag
    15481 tctgtcattg cttatgcact gcgacgaaag ctgacctatg caggaaaaaa agtaacgctt
    15541 ggcagaaacc agccacaaga agccattaca gcagttagga gtgaatttta tggcatatcc
    15601 aattaaatat attgaaaaca atctggtctg gaataaagat ggggaatgtt acgcttacta
    15661 tgagcttgtt ccctacaatt actcatttct aagtccggaa cagaaaatac aagtacatga
    15721 ttctttcaga cagcttatcg cacaaaatcg tgatggcaag attcatgctt tacaaatcag
    15781 tacagaatcc agcatacttt ctgcacaaga acgttccaaa aatgaagtca ctggaaagct
    15841 caaaacggtt gcctatgaca aaatcgacca acagacagac gctttaatat ccatgattgg
    15901 cgaaaatcaa gtggactacc gtttctttat cggttttaag ttgcttctca acgatcagga
    15961 gttttctatg aaaagtctta ccgttgaagc aaaaaatgct tttactgatt ttgtctatga
    16021 tgtgaaccat aagctgatgg gcgattttgt tagtatgagt aatgatgaaa tcctgcgttt
    16081 tcagaagatg gaaaagcttt tagaaaataa aatctcccgt cgtttcaaaa tccgaagatt
    16141 agataaggat gacttcggct atctgattga acacctttac ggacagacag gcactgccta
    16201 tgaagagtat gagtatcctc tatcaaagaa aaagctggaa catgaaacac tgattaaaac
    16261 ctatgacctc attaagccta ctcgctgtct ggtggaagaa aaacagcgat atttgaaaat
    16321 ccagcaggaa gacgaaaccg cctatgtagc ttactttacc atcaacagta ttgtcggcga
    16381 actggacttc ccgtcctctg aaatcttcta ctaccagcaa cagcaattta cctttccgat
    16441 tgatacgtca atgaatgtgg aaattgtagc gaatcgtaaa gccctatcta ctgtccgcaa
    16501 taaaaagaaa gaactgaaag acttggataa ccacgcttgg caaagtgata atgaaaccag
    16561 ctctaatgtg gcggaagctc tggaaagtgt gaacgagctg gaaaccaatt tagaccaaag
    16621 caaggaatct atgtataagc tgtcctatgt tgtaagggta tcagcaaatg accttgacga
    16681 acttaaacgt cgttgtaatg aagtgaaaga tttctatgat gatttgagcg tgaaactggt
    16741 acgacctttt ggggatatgc tgggcttaca tgaagaattt ttacctgcca gcaaaagata
    16801 tatgaatgac tatattcaat acgtgacctc tgatttcctc gctggtttag gttttggtgc
    16861 tactcaaatg ctgggtgaaa atgaggggat ttatgttggc tacagcttag atactggacg
    16921 caatgtctat ctgaaacctg ctcttgccag tcaaggggtt aagggttcag taaccaatgc
    16981 gttagcgtct gcctttgtcg gttcgctggg tggtggtaaa tcctttgcga ataaccttat
    17041 cgtctattat gcagtgcttt atggggcaca agcagtgatt gtagacccaa aagcagaacg
    17101 tggcagatgg aaagaaacct tgccagagat ttcccatgaa atcaatatcg tcaatctgac
    17161 ttctgatgag aaaaacaaag gcttacttga cccttatgta attatgaaaa ataccaaaga
    17221 ttctgaatca ctggctattg atattttgac attccttacg gggatttcct ctcgtgatgg
    17281 ggaacgcttc ccaatcctta gaaaagccat tcgtgcagta accaatagtg aagtgcgagg
    17341 gttgatgaaa gtgattgaag aattacgggt tgagaatacg ccactaagta ccagcatagc
    17401 cgaccatatc gaaagtttta cagactatga ctttgcccat ctgcttttta gtgatggtta
    17461 tgtagagcag tctatcagcc ttgaaaaaca actgaacatt atacaggttg ccgacttggt
    17521 acttcctgac aaggaaactt cctttgagga atataccaca atggagttac tatcggtagc
    17581 aatgctgatt gtcattagta cctttgcgtt ggactttatc catacagacc gaagtatttt
    17641 caagattgta gacttagacg aagcatggag cttcttacag gtggcacaag ggaaaacact
    17701 atctatgaag ctggttcgtg ctggtcgtgc tatgaacgct ggggtatatt tcgtgaccca
    17761 aaatacagat gacctcttag atgaaaaact gaaaaataac ctcggcttaa aatttgcctt
    17821 tcgttccact gaccttaacg agattaaaaa aacccttgcc ttttttggtg tagacccaga
    17881 ggacgaaaac aatcagaagc gattgcgtga tttggaaaac gggcaatgcc ttatcagtga
    17941 tttatatggt cgtgtcggtg tgatacagtt ccaccctgta tttgaagaac tgctccatgc
    18001 ctttgatacc agaccacctg tgcgaaaaga ggtgtaaatg tgaaaccatc aatattaaac
    18061 agaataaaat caaactggac gctgaaacgt ctaggtaaag tggcaatgac agtggctttc
    18121 acacttgtga ttgccatttt tcttttagcc atgctgggaa cggtggttca agctgcgggc
    18181 ttggtagatg atacggtcaa tgtggcaaat gaatacagcc gatacccact tgaaaactat
    18241 caactggatt tttatgtgga taatagctgg ggctggctac cgtggaactg gtcggacgga
    18301 attggaaaac aagtcatgta tggactatat gccattacca attttatttg gacaatcagt
    18361 ctttatgttt ccaatgcgac gggttactta gtacaggaag cctattcctt agacttcatt
    18421 tccgctacag cagattccat tggcaagaat atgcagaccc ttgcaggggt tacgcctagt
    18481 ggactatcaa cagagggttt ctatgttgga ttccttttac tcttgatttt ggttcttggg
    18541 gtttatgttg cctatacagg actgataaag agagaaacca caaaggcaat tcatgccctc
    18601 atgaattttg tgctggtttt tatcctatcg gcttccttta ttgcctacgc tcccgactac
    18661 attaaaaaaa tcaatgactt ttcatcagac atcagcaacg ctagtttgtc actgggtaca
    18721 aaaattgtca tgccccattc agatagtcaa ggcaaggaca gtgtggactt gatacgtgac
    18781 agcctatttt ccatacaggt tcagcaaccg tggctactgc ttcaatataa cagttcagac
    18841 attgaaagta ttggtattga ccgtgtggaa agcctgctct caaccagtcc agattccaac
    18901 aatggggaag acagagaaaa aattgttgcg gaagaaattg aagatagaag caataccaat
    18961 atgacgatta caaagacgat taaccgttta ggtacagtct tcttcctatt tgtcttcaat
    19021 attgggattt ccatatttgt attcctatta acaggaatca tgattttctc gcaggtactt
    19081 tttatcatct atgctatgtt tctgcctgtg agctttattt taagcatgat tccatcattt
    19141 gatggtatgt caaaacgagc cataacaaag ctctttaata ccattttgac acgagctgga
    19201 atcacattga ttattacgac agcatttagt atttcaacca tgctctatac cttatcggct
    19261 ggttatccgt tctttttgat tgcttttcta cagattgtga cctttgcagg aatctacttt
    19321 aagctgggcg atttaatgag tatgttttct ctacagagta acgattctca aagtgtggga
    19381 agtcgtatga tgagaaaacc tcgtatgctt atgcacgctc acatgcaccg tctacagcgg
    19441 aaacttggac gctccgtgac tgctttaggg gctgggtctg ccattgccag tgctacgggt
    19501 aaaaaaggac agtcgggttc gggaagttct gcgagtacac aagcagatca ctctcgacca
    19561 aacgggcagg aaaaatcaac actaggaaag cgtatcggtc aaaccatcgg tacagtagct
    19621 gataccaaag acagaatggt agatactgct ggtaatttga aagaacaggt taaggatttg
    19681 ccgaccaatg caagatatgc agtatatcaa ggaaaatcta aagtgacgga taatgtccat
    19741 gatttaacca gaagtatttc tcaaaccaaa gcggataaag ccagtggacg caagaaacag
    19801 caggaacaaa gacggaaaac cattgcggag cgtcgctctg aaatggaaca ggtcaaacag
    19861 aaaaaacagc cagcttcttc cgttcatgaa agaccagcca ctaagcaaga acaatctcat
    19921 gatggacaga cttcaaggca aacaactgta caggcttcct atagggaatc tcaacaagcc
    19981 aaacaagagc gtccaacagt taagtccgat tcttcaagtt taaaaacgga acgtcaaagt
    20041 aatacagttc aagaaagaac ggttcaaaag ccagtaactt caactgcacc aacagataga
    20101 gcttcacaac gttcaatcac aaaagaacgt cagtctactg ttcaaagagt accactacaa
    20161 aatacaaaaa gtagaccacc aatcaaaacc gccaccatta agaaaggcag taagaaacca
    20221 tgaagctgaa aactttagtg attggtggtt cgggattatt cttgatggtc ttctcactgc
    20281 ttctgtttgt cgccatttta ttttcagatg aacaagatgg cggtttttcc aacattcact
    20341 atggcggtgt ggatgtttcc gcagaagtgc tggctcataa gcctatggta gaaaaagttg
    20401 ccaaagaata tggaattgaa gaatacgtca atatacttct tgcgattata caggtggaat
    20461 cgggtggtac agcagaagat gttatgcagt cctcggaatc cctcggtctt ccacctaatt
    20521 cattgagtac agaagaatcc attaagcaag gtgtgaagta tttcagtgag ttattagcca
    20581 gtagcgaaag gcttggtgta gatttagaat cggttatcca gtcctacaat tatggtggtg
    20641 gtttcttagg atatgtggct aatcgtggga ataaatatac ctttgaactg gctcaaagtt
    20701 tctcaaaaga gtattcgggt gacgaaaaag tgtcttaccc caatcccata gccataccta
    20761 tcaatggggg ctggcgatac aattacggga atatgtttta tgtgcaactg gtaacgcagt
    20821 atcttgtcac aacagagttt gatgatgata cagtacaagc catcatggac gaagctctga
    20881 aatttgaagg ctggaagtat gtctatggcg gagcttcccc gactacctct tttgattgta
    20941 gcggactgac acaatggacg tatggaaaag ctggaatcaa cttaccacga acagcacaac
    21001 agcaatatga tgtaacccgg catatcccat tattggaagc aaaagccggc gatttggttt
    21061 tctttcattc tacctataac gctggctctt atattactca tatcgggatt tatttaggcg
    21121 ataaccgtat gtttcatgca ggcgacccta tcggttatgc cgacttaaca agctcttact
    21181 ggcaacagca tttagtggga gcaggacgaa tcaaacaata agaaaggacg attcaatgat
    21241 gaaattcgga aaaaatcaga atacagaaaa acagacacca aaggaaaaga aacctcgtgt
    21301 ctataaggtc aatcctcgta aaaaggttgt gattgtccta tgggtacttt taggacttag
    21361 tttcagcttt gcgatattca agcactttac agccattgat acccatacca ttcatgaaac
    21421 caccatcata gaaaaggaat atgtcgatac tcatcatgtg gaaaattttg tagagaactt
    21481 tgcgaaagtc tactattcat gggaactaaa cgacaagtcc attgataatc gcatagaaaa
    21541 cctgaaagca tatctgacag aggaacttca agctctgaat gtcgatacag ttcgtaaaga
    21601 tattcctgta tcgtcttctg taaaaggatt tcagatatgg acggtagaag cagatggcga
    21661 caatcaattt gatgtaacct acagtgtaga ccaactcatt acagaggggg aaaatacaaa
    21721 gaccgtccac tctgcttata tagtgagtgt ctatgtagac agtactggaa atatggtcct
    21781 cattaagaat ccgaccatta ctagcatacc aaagaaatca gactataaac caaaagctat
    21841 tgaaagtgag ggtacggttg attccattac aaccaatgaa atcaatgagt ttttaacgac
    21901 gttcttcaag ctctatccta cagcgacagc cagtgaactt tcctactatg tgaatgacgg
    21961 gatattaaaa ccaatcggaa aagagtacat ctttcaagaa ctggtaaatc ctatttacaa
    22021 tcgtaaggat aatcaagtca cggtatcgct gacagtggag tatatcgacc agcagaccaa
    22081 agcaacgcag gtatctcaat ttgatttggt acttgaaaag aacgggagta attggaagat
    22141 tatagaataa caaatattgg tacattatta cagctatttt gtaatcacgt actctctttg
    22201 ataaaaaatt ggagattcct ttaca
//
LOCUS       DAKBVG010000019        22055 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000019 DAKBVG010000000
VERSION     DAKBVG010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 22055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22055
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.019"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            62..790
                     /locus_tag="OUD09_000576"
     CDS             62..790
                     /locus_tag="OUD09_000576"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCT9691874.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            790..1044
                     /gene="purS"
                     /locus_tag="OUD09_000577"
     CDS             790..1044
                     /gene="purS"
                     /locus_tag="OUD09_000577"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HCT9691875.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1046..1717
                     /gene="purQ"
                     /locus_tag="OUD09_000578"
     CDS             1046..1717
                     /gene="purQ"
                     /locus_tag="OUD09_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HCT9691876.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            1718..3940
                     /gene="purL"
                     /locus_tag="OUD09_000579"
     CDS             1718..3940
                     /gene="purL"
                     /locus_tag="OUD09_000579"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HCT9691877.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            3925..5364
                     /locus_tag="OUD09_000580"
     CDS             3925..5364
                     /locus_tag="OUD09_000580"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCT9691878.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            5387..6439
                     /locus_tag="OUD09_000581"
     CDS             5387..6439
                     /locus_tag="OUD09_000581"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCT9691879.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            6436..7014
                     /gene="purN"
                     /locus_tag="OUD09_000582"
     CDS             6436..7014
                     /gene="purN"
                     /locus_tag="OUD09_000582"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCT9691880.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7011..8546
                     /gene="purH"
                     /locus_tag="OUD09_000583"
     CDS             7011..8546
                     /gene="purH"
                     /locus_tag="OUD09_000583"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCT9691881.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            8562..9836
                     /gene="purD"
                     /locus_tag="OUD09_000584"
     CDS             8562..9836
                     /gene="purD"
                     /locus_tag="OUD09_000584"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCT9691882.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            9958..10872
                     /locus_tag="OUD09_000585"
     CDS             9958..10872
                     /locus_tag="OUD09_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCT9691883.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11005..12570)
                     /locus_tag="OUD09_000586"
                     /pseudo
     CDS             complement(11005..12570)
                     /locus_tag="OUD09_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(12563..14014)
                     /locus_tag="OUD09_000587"
     CDS             complement(12563..14014)
                     /locus_tag="OUD09_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCT9691884.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLWENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14028..15044)
                     /locus_tag="OUD09_000588"
     CDS             complement(14028..15044)
                     /locus_tag="OUD09_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCT9691885.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15264..16352
                     /locus_tag="OUD09_000589"
     CDS             15264..16352
                     /locus_tag="OUD09_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HCT9691886.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            16406..17932
                     /locus_tag="OUD09_000590"
     CDS             16406..17932
                     /locus_tag="OUD09_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCT9691887.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            17934..19139
                     /locus_tag="OUD09_000591"
     CDS             17934..19139
                     /locus_tag="OUD09_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCT9691888.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            19396..20373
                     /gene="guaC"
                     /locus_tag="OUD09_000592"
     CDS             19396..20373
                     /gene="guaC"
                     /locus_tag="OUD09_000592"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCT9691889.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            20637..>22055
                     /locus_tag="OUD09_000593"
     CDS             20637..>22055
                     /locus_tag="OUD09_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCT9691890.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLT"
BASE COUNT         7105 a         3723 c         5035 g         6192 t
ORIGIN      
        1 tgaaaaaacg aacaaaaaac gatgtatttg cttttaatgt aaataaaaag gagagacgta
       61 catggagaag aaagaacttt tgtatgaagg aaaagccaaa aaaatatatc ttacttctga
      121 tgagaacttg atttggatag aatatttaga tcaagcaact gcgttgaatg gcgcgaggaa
      181 agaccagata agtggaaaag gagaattgaa taatcaaatc acaagtgcaa ttttccgtta
      241 tctgactcaa agaggaataa aaaatcattt tattgaacaa ttgtctaaaa ccgaacaatt
      301 agcaaaaaaa gtacaaatta tccctctgga agtggttatc cgaaatattg cagcaggaag
      361 tttctcaaaa cgtctaggtg ttcccgaagg taaagaattc atcaaaccaa tcattgaatt
      421 ctactataag gatgaccagt tggatgatcc atttatcaat gaagagcata tctatttctt
      481 acaacttgca aagccggaag aaatcagtga gctgaaagcc caagcgttgg ctattaatga
      541 aattttgacc gaattatttg ctaaggtcgg tttggtttta gtagatttca agctagaatt
      601 tggccggtcg aatggacaaa ttttgcttgc ggatgaagta acaccggata cttgccgatt
      661 atgggatgaa cgtacgaatg aacatatgga caaagacgtt taccgcagag atctaggtga
      721 tttgatccca gtctatcaag aagtatataa ccgtctatcg gaactaactg aagaaaagga
      781 gcgagtatga tgtatttcgt aaaagtatat gtaacctata aagattcagt gttagatcct
      841 caaggggagg cagtaaaaaa agcagtccat cgtatgggat atgaaacaat cgaagatatc
      901 cgaataggaa aatactttga gatacaactt gctgatgcag aagaaccaat cgatacagtg
      961 attgaaacga tttgtgacaa gctgcttgca aatgtaaata tggaaacata ccgatatgaa
     1021 atcaaaaaaa tggaggaagt ataacatgaa atttgcggtc atcgtttttc caggttcaaa
     1081 ttgtgacgca gatatgttat gggcagttcg cgagatcatg ggcgctgatg ccgaatttgt
     1141 acggcatgat gagtcctctt tagaaggatt tgatggggtt ttactgccgg gaggattctc
     1201 ctatggtgac tatttgagat gcggtgcgat tgctcggttt tctacaatca tggaagaagt
     1261 gattcgtttt gctgaagaag ggaaaccagt attcggtaca tgcaatggat tccagatatt
     1321 gacagaagca ggacttttgc caggtgtact gagacgaaat gagtctttgc attttatttg
     1381 caaaactgta ccattgaaag tcaaccccaa gacggccttt acttcagagt atgaagccga
     1441 cgaaataatc tatcttccag ttgctcatgg ggaaggaaat tatttctgtg acgaagaaac
     1501 acttcaagaa ctaaaaaaaa ataatcagat tgcctttact tataatgaca acatcaacgg
     1561 cagcgtggaa aacattgcag ggattacgaa caaagcaggg aatgtgttag gaatgatgcc
     1621 tcatccagaa cgtgcagtag aagacttgct aggatcagaa gatgggaaac ggttctttgc
     1681 ttccatttta aaaaacttcg gaaaggtgac cgtatagatg aaaacattga aagaaccgac
     1741 agctgaagag atcaagaaaa aaagaatcta tgccgaatgg ggattgactg atgaagaata
     1801 tcgaatgatc gaagaagata tcttaggaag aatgcctaat tatacggaaa ctggactgtt
     1861 ttctgttatg tggagcgaac attgttccta taaaaattct aaaccggttc tacgcaaatt
     1921 tcctactagt ggacctcatg tgttgcaagg ccctggagaa ggcgcaggca tcgtcgacat
     1981 cggtgatgga ctagcagttg tcttcaaagc agaaagtcac aatcatcctt cagccgtgga
     2041 gccttatgaa ggagctgcga caggagttgg cggaatcatc agagatattt tcagcatggg
     2101 ggctcgtcca attgcgttgc tagattcatt gaggttcggc gaattagata atccaagaac
     2161 gaaatattta ctagaagaag tagttgcagg tatcggcgga tacggcaatt gtatcggcat
     2221 accgactgta ggcggcgaaa ttgcatttga tccttgttat gaaggaaacc cattagtcaa
     2281 tgcaatgtgc gtaggtttga ttgaacacaa acatatccag aaagggcaag ccgctggcgt
     2341 cggtaattcg atcatgtatg taggtgcaaa aacaggtcgc gacggtatcc acggagcaac
     2401 ttttgcctca gaagaattta atcaagaaga agaacagcaa cgttctgctg tacaagtagg
     2461 cgatcctttt atggaaaagc tgctgcttga agcgtgtttg gaattaatct tgcatcattc
     2521 ggattctcta ataggtatac aggatatggg agcagcagga cttgtttcat ctagtgcgga
     2581 aatggcttca aaagctggct caggacttat attgacatta gatgaagtcc cacaaagaga
     2641 aactgggatg acaccttatg aaatgatgtt atcggaatca caagaacgga tgttgatttg
     2701 tgttaaatca ggagaggaag aaaaaatcca agaactattt cagaagtatg atctagatgc
     2761 agtcactatc ggtaaagtaa cagacgacgg gcaatatcgg ctatatcatc aaggagaaga
     2821 agtagcaaat ctgccagtcg atgcattagc tgaagatgca ccagtttatc ataaagaaat
     2881 gaaagaacca gctcgaatcg tagaatttca gcagttagct ccttatcagc cagttatcga
     2941 agaacctgga caagtattgc ttgatctgct gcagcaaccg acgattgctt caaaacgttc
     3001 aatctacgaa acttatgatt cacaagtaca aaccaataca gttgtccgtc caggaagcga
     3061 tgctgctgtg atgagggtta gagggacgaa taaagcgtta gctatgacca cagactgcaa
     3121 cgcgcgctat ctgtatttgg atccaaaaat cggaggacaa atagcagtgg cagaagctgc
     3181 tagaaatatt attgctagcg gtggtaagcc cttggcgatc accgactgcc tgaactatgg
     3241 ttctcctgat aaaccagaag ttttttggga attatcgact tcagctgacg gaattgctgc
     3301 tgcgtgtgaa acgttaggaa ctcctgttat ttcaggaaat gtctctttat ataatgaaac
     3361 ggatggtcaa gcaatctatc caacaccaat gattggtatg gtcggattga ttgaggatca
     3421 taaacatatc actactcagg aattcaaaaa atcaggggat ctaatctata tattaggtaa
     3481 gacgtttgca gattttgatg gcagtgaatt acaaaagatg cagcttggaa gaatcgaagg
     3541 tgtgatccga aactttgatc tatccattga aaagagaaac caagagttag tattgacagc
     3601 catccaaaac ggattgattg aaagtgctca tgactgttca gaaggtggct tggcaattgc
     3661 actggcagaa tcagctttca aacatcagct agggatatct gtacagtttg aattgtcctc
     3721 tgctcaatta tttgcagaaa ctcaatcaag atttgtgttg acagtcgcac cggaaaataa
     3781 gacacgattc gaagaaatga tgggagatgc tgctgtttta gcaggtaagg tgacggatga
     3841 agcgataatc gaaatttcag caactgacgg tcagatcaaa atagaaactg cagtagcaag
     3901 aaagtgctgg gaggatgcaa tcgtatgtct tatgaagtga agagtttaaa tgaagaatgc
     3961 gggatcttcg gtgtttgggg tcatccagac gccgctagtg tggcatactt cgggctccat
     4021 agtcttcagc atcgaggaca agaaggtgca ggtatcgtgt ccaatgatcg aggaaagctt
     4081 aaggggcatc gagatttggg gctgctttct gaggtattca aagatcaaag aaaattgcag
     4141 cagttaacag gagaagctgc aatcggacat gtacgttatg caacagcagg caatggaagt
     4201 gtggacaata ttcagccttt tctattcaaa tttcatgatc agtcgatcgg cttagcgcat
     4261 aatggtaact tgatcaatgc tcgttccctt agaaaagagt tagaggaaaa tggggcgatc
     4321 ttccattcta actccgatac ggagatattg atgcatttga tccgacgaag tgaagaaaca
     4381 gattttttaa ataaattaaa aagtgctttg aatgaagtca aaggtggctt tgcctattta
     4441 ttaatgacag aaacggcaat gattgccgct ttggacccaa atggttttcg tccattagca
     4501 atcggacaaa tgaaaaacgg agcatacgtg attgcctcgg aaacttgtgc attagaagtt
     4561 attggggcac gttttataag agatgtccag cctggtgaga tagtgatcat caatgacgaa
     4621 ggaatccaga ttgattgctt tacgaaggac acgcagcttt ctatttgttc gatggaatat
     4681 atctattttg ccagaccaga ttcaaacatc gcagggatca atgtgcatac tgcacggaaa
     4741 aatatgggac ggaatttagc gaaagaatcg ccagtaaaag cagatatggt cgttggcgta
     4801 cctaattctt ctttgtcagc cgcaagcggt tatgcggaag ctagtggaat cccttatgaa
     4861 atcggattag taaaaaatca atacattgcc cgaacattta tccagccaac gcctgagcta
     4921 agagagcaag gtgttcgaat gaaattatct gctgtacgag gagtagtgga aggtaaaaag
     4981 gtgattatgg tcgatgattc aattgttcgg ggtacgacaa gcagacggat cgtccatctt
     5041 ttgaaagaag caggggcaaa agaagtccat gtgaggattg cttctcctcc tttgaaatat
     5101 ccatgttttt acgggattga tattcaaacg cgtaaagaac tcattgcggc aaatcacacg
     5161 attgatgaaa tcaaagaatg tatcggtgca gattctttag gttttttaag tgaagagggt
     5221 ctgatcgaat cgatcggtct ggaaaaagat gctccgtatt caggattgtg tatggcttat
     5281 ttcaacggag attatccgac gccgttatat gactatgaag aaaaatatca ggaatcacta
     5341 aaagaaaaag tatcgttctt ttaaaagcag accaagaagg gagtatatga attcaatggc
     5401 aaatgcatac gcaaaagctg gcgtagatgt cgaagctgga tatgaagtag tagaacggat
     5461 caagaagcat gtcaaaaaaa cggaacgttt aggtatgatg ggcgctatag gaggattcgg
     5521 cggttgtttt gatttatcgg tctttcaatt aaaagagcca gtccttgtct cggggaccga
     5581 tggcgtgggg acgaaattag cagtggcaat cgaagcagac aaacacgata caatcgggat
     5641 cgactgcgta gcgatgtgtg tcaatgatat cttagcacaa ggcgcagagc cgttatattt
     5701 cttagactac ttagcaacgg ggaaaaatga tccgttacgt ttggaaaatg tagtaaaagg
     5761 tgtagcagat ggatgtgttc aaggaaatat ggcgctgatt ggcggagaaa cagctgaaat
     5821 gcctggtatg tactcaggta atgattatga tctagccgga tttgctgtag ggatagctga
     5881 aaaatctcaa ttattgacag gagaaacgat caacccagga gatgtgttgc ttggaatagc
     5941 atctagcggg attcattcaa atgggtactc tcttgttcgc aaaattttct ttgagcagca
     6001 gcagttttct ttatcatcaa aactgccaga attgaaaaaa acacttggcg aagaattgct
     6061 tgagccaacg aaaatctatg tttcttcctt gcttccgctg atcaaagaaa agaaaataaa
     6121 aggagctgca catatcactg gtggagggtt tgtagaaaat atcccgcgaa tgttaccaga
     6181 atctatggct gcacaaatca aacttggctc atggccgatt cttccaatat tcggctgttt
     6241 acaaaaatat ggtgaaattc ctgagatgga gatgtatgaa atcttcaata tgggcatcgg
     6301 tatggtcctg gcagtcgaag aatcgaaagt aaaagaaata gtacacgaaa ttgagcaagc
     6361 tggcgaaaaa gtttatgaaa tcgggaaagt gatcgccaaa gaaaaacatc gtgtggtgtt
     6421 tataggagaa gaacgatgag aatcgctgtt tttgcttctg gaaacggaag caactttcaa
     6481 gcgctagccg attatctatc gaaaaaagga ttggaatctt cgattgactg gttgttttgt
     6541 gatcagcctg aagcatatgt tttgaaacgt gcaactgcgt tgagtgtacc ggcagattgt
     6601 ttttcaccta aagaatttga ttcaaaaaaa gagtatgaag aagcaatttt gcataaattg
     6661 aaagaaaaaa agatcgattt gatcgtatta gctggatata tgcgaatcat tggaccggta
     6721 ttgctgaaaa attatgataa aagaatcatc aatatccatc cgtctctttt acctgccttc
     6781 cctggtttgc atggcatacg tgatgccttt gaagctggag taaaagaaac gggtgtcaca
     6841 attcattata tcgaccaagg tgtagatact ggaccaatca tccggcagga aaaagtgaga
     6901 attgaacagg aggacacgtt cgattcttta gaagaaaaaa tccatagagt agagcatcga
     6961 atctatccag aagtgattag tgaaattatt gaaaatggag gctatctgaa atgacaagag
     7021 cattgatcag tgtatcagat aaaactggga ttgtagcatt cgcccaaggt ttgattgaac
     7081 aaggagtaga aatcatttca acaggaggaa caaagcatgc gttggataaa gctgggatca
     7141 aaacgctttc gatcgaagaa gtgacagggt ttcctgaaat gatggatggc cgtgtcaaaa
     7201 cacttcatcc taaaatccat ggtggtcttt taggaagacg cgatctccct agccatatgg
     7261 aggcaatgga aacggaaaat atccaaccaa tcgactttgt ttgtgtcaat ttatatcctt
     7321 tcaaagaaac gattatgaaa ccagatgtcg aaatagcaga agctattgaa aacattgata
     7381 tcggtggtcc tagtatgctc cgtagtgcag caaaaaacca tgaatttgtc acctctgttg
     7441 ttgatccatc tgactatgaa gaagtacttt cagaattacg tcagtatggg catacttcac
     7501 tttctttcag acaaaaacta gcggcaaaag tctttcgtca tactgcggca tatgatgcac
     7561 tgatcgctca atacttaacg gattgggtgg aagaaaaaga accggaaaaa ttgacactta
     7621 cgtatgaacg aaaacaaacg cttcgttacg gtgaaaatag ccatcaagcc gctgcctttt
     7681 atcagtccgc tcttctggaa aaatattcga tcgcagcagc aaaacaactc catgggaaag
     7741 aactttctta taataatatc cgagatgcag atgctgcgct acggatcatg aaggaatatg
     7801 acgagccaac ggtagtcgcc ttgaaacaca tgaatccttg tggtattggg acagcatcta
     7861 cgattttaga agcgtgggaa gcagcgtatg aagcagatcc tgtttcaatc tttggtggga
     7921 tcatcgtttt gaatcgagaa gtagatctgc caacagcaga acagatgcat aagctgtttc
     7981 tagaaatcat cattgcccct tcttatacac aagaagcttt ggaattactg caaaaaaaga
     8041 aaaacatccg attactgaca gtagactttg cgcaggaaga aaatcaaaat cgagaagaaa
     8101 cggtctcagt cttaggtgga cttcttgtac aagatcaaga tctggcaata gaaaatgaag
     8161 aagagtggaa agtcgtgaca aaacgtaagc cgactccagc agaagcaaaa gcattagcgt
     8221 ttgcttggag agctgtcaaa catgtgaaat ctaatgcgat cgtactggca aacgaacgtc
     8281 agacagtagg aatcggagcc gggcagatga atcgagtcgg ttcagtaaaa attgcgattg
     8341 aacaagcgca agagaaaatg gaaggtgcag ttttagctag cgatgcttat tttccaatgg
     8401 atgattcggt tgaatatgca gccaaacatg gtataaaagc aatcattcag ccaggaggaa
     8461 gcatcaaaga ccaagattct attgatatgg cgaataaata cgatctagca atggtcttta
     8521 ctgatgttcg gcattttaga cattagagag gagtcggata aatgaacatt ttggtgatcg
     8581 gtgcaggtgg tcgtgaacat gcaataagta aaaaactttt agccagtccg aaggtggata
     8641 cggtgtattg tgcgccagga aatccgggga tgacaaaaga tggtatcaat tgtgtcgcta
     8701 tcagtgagcg agatcattca gcgttaatcc attttgttaa gaaagaggct attgattgga
     8761 gtatcgttgg tcctgaagtt cctttgttaa atgggatcgt cgatgatttt atggcggaag
     8821 gattgaaaat cttcggtccg acaaaagcag cagctctgat cgaaggttcg aaaagttttg
     8881 ctaaacaatt aatggatact tatcaaatcc cgacagctaa atccagaagt tttgaatgtt
     8941 acgaaaaagc agcagcgtat atcaaagaaa atggtgcgcc aatcgtagtg aaagcggacg
     9001 gcttagcagc tgggaaaggt gtagtcgtgg ctaccacagt gaaggaagcg ctggatgcgg
     9061 cagaacaaat gttgaaccag catcgatttg gggatagtag caaaaaaatc gtcgtagaag
     9121 agtttttggc aggagaagaa ttctcccttt tggcgtttgt ccgtaatacc aaggtgtatc
     9181 cgatggtcat ttcgcaagat cataagcggg catttgataa cgataaaggg ccaaatacag
     9241 gcggaatggg tgcttattca ccagtaccgc aaatttcaaa aaagatgatt gaaaaagctg
     9301 tggaagaagt tttaaaacca gcggctttgg gaatgaaaga actaggacgc tctttcacgg
     9361 gtgttttata tgcgggtttg attgctacga aggaaggacc aaaagtgatc gaattcaatg
     9421 cgcgtttcgg tgatcctgaa acgcaagtcg ttctttctcg gttgaagagt gactttgcgc
     9481 aagtaatcga tgatttatta gagaatcgta cagtagagtt aaagtggcag caagaaggat
     9541 acaatgtagg cgttgtggtt gcagctgccg gttaccccga agaatatgag acaggaatgg
     9601 ttctaccaga ttttctggaa gaagagcttt ctgtttacta tgctggagtt tcctcaaata
     9661 acggcaaact agttggagca ggaggacgtc tttatttagt agaagcatac ggtgagacga
     9721 tcaaagaagc acagaaaaaa atttacaccg cgttaatgga taaaacgaca gatggaacat
     9781 tttatcgtac agatattgga gaaaaagctg ccaatagcga actgatcgtg aagtaaatca
     9841 gctgcttcca tttataaaaa atacaacaaa cataatcgga agcgccgatt ttagtgaagc
     9901 cttttcataa agagtgtggt acacttttgt tgtgtatata aataaggagg agttcagatg
     9961 aaaaaaattc aattaggtac ttctgatcag aaagcagctg cagtgatctt aggctgtatg
    10021 cgtatcaaca atgcggaaga tccagtaaaa gtgattgaga cagcttatga aaacggcatt
    10081 gatttttttg accatgcaga tatttatgga aacggtgaat gtgaagaaat ctttgcagat
    10141 gctctagcaa aaacatcaat caaacgagaa gatcttttta ttcaaacaaa atgtggaatc
    10201 gttccaggtg tgatgtttga tttttctaaa aaacatatca ttgaatctgt agaaaaaagt
    10261 ttgacccgtc tgaaaatgga ttatgtggat gctttacttc ttcatcgtcc agatacattg
    10321 gtagaaccag aggaagtagc tgaagcgttt gatgaattgg aacggtcagg aaaagttcgt
    10381 tattttggcg tcagcaatca aaaaccaatg caaatcgaat tgctgaaaaa atatgttcgt
    10441 caaccattag tagctaatca gctgcaattt ggcttgaaac atactggaat gatcgatcaa
    10501 gggattcatg tcaatatgac agatgaagca agtatcgatc atgacggtag tgtacttgat
    10561 tattcccgat tgcatgatat gacgatccaa gcatggtcac cataccagta tggatttttt
    10621 gaaggtgtat ttatcgggaa cgaaaaattc cctgaattaa accaaacatt agaagaacta
    10681 gcagctaaat atcaaacgac accaactggc ttggcttctg cttggatttt acgccatcca
    10741 gccaatatgc aagtgatcgc cggtacgatg aacgtagggc gaatcaagga aatcgctcaa
    10801 gcttcagaaa ttaatatgtc tcgggaagat tggtacgaat tgtaccgtgc tgctggaaac
    10861 attttacctt aaagattgtc ataagcaggt caaaagggct ataaattaaa aagacagaat
    10921 aacttgaaag ctgttttcag cttcgagata ttctgtcttt ttttattgca aatggaaaga
    10981 gtatcattgt acggatttcg tttattagtt atttttgttt ttaaggaaaa gctgtgtccg
    11041 atattctttt ggagaaatcc cttgtttttt cttaaataaa taactgaaat agttagggtc
    11101 attataacca atttcaaaag caatatctga aatacgatag tctgattgct gcaataattt
    11161 ttttgctaat tcgattcttt gttctgtcag ataatcgatg aatgtttggt taaacgattg
    11221 agaaaaaatc gtactgaaat gagagggact taatgcaact tgttgagcga ctcaagttaa
    11281 ggacatatct ggatcggtaa aattttcttt gataaaatcc aatgcatggt tcaaaattgg
    11341 ctgatatttg ttccttgaag gctgtgaggg cagagcagtt aagaaagaaa tgaacatata
    11401 aactgtcgaa cggtaaagcg agtcgtcggc gctgattgct aagcgttgac tggtggatgt
    11461 gcaatgaacc atttcttgcg ggattttctt ttctttcagg cagacatgct ggagtagctt
    11521 agtcaagaca aataaacgat ataatcgtgt tcgagttggt gtgccttgtg gtttcgtcaa
    11581 gtgttcgatg tacttagata cttcgttttt cgataatgaa gacaaatcaa gtgagaggtc
    11641 aaagtaaaag acagaatcaa ctggaagttt cgcttcttga atcaaatcct catagctgat
    11701 aatttgttct ttaagggaac tggcggtttc aagtatcatc tgcgtgatct caaaagatgc
    11761 aggtaattcg ctgattcttt ctgcgacagg accaatggcg acagaaaaat tatttttttg
    11821 tcccttttct aattcatgga ccaacgtttg ggctacctga taactggtct cgatcacatt
    11881 ttcttcaaga gaatcaacta ttaaaaattt gatgaatcga gaggaaacag cagagaagat
    11941 cacttgttga ttctccctaa ataaaaactt caaataatca ctgagatgag cgtaatcttc
    12001 aaagtctgta ctgacttggt ttgctgctaa taatacggtc atttttttcc ctgctaaaga
    12061 gcgattgaaa gattcagcaa gtttcaatgt ttcactgatc gtaagtgagc ctttgaacaa
    12121 accgttcaag aaatgatttt ttttcaattc gaaaacaaga tcagaagaag tgtgcgtttc
    12181 tttttgctgc aaatctaaag cagttactgc tttttctatt gcttgtttca attcgctact
    12241 tttaatcggt ttaagcagat aatcatctgc ctgtacctgg attgctcctt tcatataatc
    12301 gaaatcgtcg aatccactaa taaatatgag acgagtccaa ggaagtagtt tccttgcttc
    12361 ttttgaaaaa gtcaacccgt ccatgaaagg catttggata tcagtcagaa tgatgtcggg
    12421 ctgtaagtcg ataattgaag acagtgccat ctcaccatct ccagcttcgc cgacataagt
    12481 gattggataa taagaaccaa ggacagttat ttgttcccgc aagttattgc gaatgaggta
    12541 ttcgtcttcg atgataaaaa ctttatacat gtttgtttcc tccaattgtt ttgggagata
    12601 gagggatcat cagtgtgaca gttgttcctt ctcggtattt gctgtcaatc gtgataggtt
    12661 tttgatcatt tccgtaataa agaaggagac gcttgtttac gttgtaaagt ccgtagccga
    12721 tactgaaatc tgaatcgatc cctttggcta actctttacg tatttctgcc agtttttctg
    12781 cagggatgcc aatcccgttg tctataactt tgaattggat catctcatca attttgcatg
    12841 tggtaatacg aataaatcct ttgtgacgga cgtacttcgt tccatggtat acagcatttt
    12901 caatcaatgg ctgcaagatc atttttaaaa tagaaacagg aaaaagtgtt tctggtatat
    12961 cgatcttata gtcaagcata tggccgtatc ttatttttaa gatgactaga taatcattga
    13021 tatggctaat ttctttttca agaggaatcc agtcttgtcc tttgcttaaa gacgtacgaa
    13081 aaaagccgga aagtgcatgg gtcatagtca gagcttcttc aaagcagtct tgttcgatca
    13141 aagaaataat ggcatccaaa ctgttataaa taaaatgggg agtaatctga gcttgcaata
    13201 cacgaacttc actttgtgct aaatggtatt gtttcaaagc gttctcccat aaagactggt
    13261 ttagatcttc cgccatttta ttcatgctag cggtaagtgt ttgtaattcc agtgtttccg
    13321 gcagggttaa acgaaagcca aggttccctg aagaaagttg ttctgacatc tcgatcagct
    13381 gattgatcgg ttcttggacc tttctctcta aaaagcgacg attctttttc gcatagaaaa
    13441 aaatggtact aagaactcca agttccacta ttgttaatag aatgagcgag cggacaagtg
    13501 tcgtattttt tttgcttgct aagtcgatct ccacacgaac aaaatcttgt aagatatctg
    13561 acagcagttg agtgacagaa tctacctgat tgatgatctc ttcattggta tccataaaat
    13621 cttttgccaa gatattattg atcagctcgt tttgataatc ttccagtgta tcgatcgtac
    13681 gtagcgatac atctaacgta ctaatttctt taagtgtatt tgtattattt ttgatctctc
    13741 gaatatcatt cctaagttct tctaggatat tttcctcatt gaactcatct acggttactt
    13801 ggccagaaat caagtcccac atactttcca aaactttttg atcaaccttc aaagcaattg
    13861 catctgcttt ttggatattt gcgatcgtct gttgataaat caaaagctgt tgcgaatata
    13921 acagtatgct cagtagcaaa gggataacag caaatagaag catggctttg tttgcttggg
    13981 caaaaatctg ttttatgctt aaattttttg tcatgatact tctccagtca ataatttctg
    14041 gttgggatgt tagctatatt ctgctgggaa aagacagtct cggaaataaa aatatcttta
    14101 ggtaatgttt ttcctgtttg aatatttcca aaataacgag taacggtatt ggctacatac
    14161 cagccgacat caggtttaca ctcgactaca caattgacga ttcctgagcg tagcttctcg
    14221 atcatatctt cttgtccatc aatagttaca atgatcaaat cttctccagg tttgattgat
    14281 gtttctttta tcgcagctaa agcaccgtga gtcatttcat cactatggct gtataataca
    14341 tcgatttcgg ataaatcatt tgtttctaaa tatcgtttca tctggtcttt tcctttcatc
    14401 cgaataaaat caccgacaat tcggtgaaca atagtcatcg gaggtttatg gtcgatggtt
    14461 ccatcttttt ttagggttgg tttacgattg attgattcca aaaatccttc ggagcgtagc
    14521 ttggtcggtg tggagttctc tgaaccagca agttctagga ttctgatttc ctgtttcttt
    14581 gaatttgtga agtgattgct gacaaataaa cccgcccgat ttccttctgc tttgaaactg
    14641 gggccaatgt gggtgacgaa aagctcggga tcattcgttt gaatatgtct gtcgacaagt
    14701 atgacaggga tccctgctcg ctttgcttct tttaatacag agtcccaccc ggattcttgc
    14761 aaaggagtaa aaatgatcat atccacttta taggcaatga acgtccgtat atcttggatt
    14821 tgccgttcct gattcatgta gccatttcgg tagagaactt ggtaatcttc tttttcaagc
    14881 gcagtgataa ttgattcagt atgctttttc cgccagctac tctccgttcc tgattgcgaa
    14941 aaaccaacga ctagtgggtc aggtgcttcc tttgtattag gtgtacaccc catcatttgg
    15001 taagcgatta caacaagggc taaaaaaaat aaaacacgtt tcactttaaa tcactccatc
    15061 tggatttgtt agtactatct tatcagataa aagggaaaat caaagtcaaa aggaatcaat
    15121 cgaaaaaaat ccaagaaact taaaaaaatc caagacaaaa aaagtacacc taaaaaattt
    15181 aaagtaaatc ccataaaaaa atcaatgtta acgctttctt tattagctat actcggtgta
    15241 tcaattgaaa tgggggataa aaaatgaaaa acaaatttag aaagattgtt gggactatcg
    15301 ctattttgac cgcaagcatt ctattaagtg cgtgtggcaa ttcaggatct gcagatgata
    15361 gtacggggta tgtaggaatc gctatgccga ccaaatcagc agaaagatgg atcgctgatg
    15421 ggaacaacat ggtctcggaa ttagaaaaac tgggttataa aacagatctt caatatggag
    15481 aagataaagt agaaaatcaa gtagcacaaa tagaaaatat gattacaaaa ggggtagata
    15541 cacttgtgat tgcttctatc gatggttcgg ctttaactga tgtgttagct aaagcaaaag
    15601 aagcagatat caaggtgatc gcctatgatc gtttactgat gaattcagaa aatgtcgatt
    15661 attacgcgac ttttgataat tttggagtag gtgtatcgca agcagcttat attgaagagc
    15721 acttaggatt gaaagaagga aaaggaccat tcacgattga attatttggt ggctcgcctg
    15781 acgacaacaa cgcactaatc aattacaacg gagtgatgtc tgttttacaa ccatatatgg
    15841 acaacggtca gttggtcgtg ccatctggac agacgagttt cagtcaaatc gctacgcttc
    15901 gctgggatgg ttctacggcg caagcaagga tggataatct gttaagcgca aattatactg
    15961 accaaacatt ggatgctgtt ttgtcgccat atgatccaat aagtttagga atcatttcgt
    16021 cattaaaagg cgtggggtat gggagtgaaa gtaaaccttt acctgtgatt actggacaag
    16081 atgcgacagt tgctggcgtt aaatcgatca ttgccgggga acaaacacaa acgatcttta
    16141 aagacacacg tatcttagca aaaaatacga ttgaaatgat caaagcaatc agtgatggag
    16201 aagaggttcc ggtcaatgat actgaaactt atgataatgg ggtgaaaact gtccctacgt
    16261 atttagcaaa tacagtatct gtcgataaag ataattatca agcagagctg atcgacaccg
    16321 attattataa agaatccgat ctgaaaaatt aagaggaacc aaacaaggaa ctaaagagat
    16381 ctaagtcaga gagaggataa gaattatggc ggattgtatt ttaaaaatgg cagatatcac
    16441 caaaaaattt tctggaatcc gagcgttaga ttgcgtgaat ctgatgattg aacgtgggga
    16501 aatccatgca ttgtgcggag aaaacggagc gggaaaatca acattgatga atgtcttgag
    16561 cggcttgtat ccatatggct cctatgacgg ggaaatcttt tatgatggaa agctgtgtca
    16621 atttaaagat ttgaaagata gtgaagaaaa aggcatcgtc atcattcatc aagaattagc
    16681 actaagtcct tatctttcaa taaaagagaa tatttttctc ggaaatgaac aagcagaaag
    16741 aggcgtgatt gattgggaac tgacagaaaa gaaaacatat gaactgctga ataaagtcgg
    16801 tttgtctatc gacccgaatc ttctagtatc ccagattggc gtagggcaac agcaactagt
    16861 tgaaatagct aaagcattat caaaatccgt ccgtctgttg attttggatg agcctaccgc
    16921 tgcattgaat gaagaagaaa gcgcgaattt gctgagattg atccgagagt tgaaagagca
    16981 aggtgtaacc tccatcatta tttcacataa gctgaatgaa atcgtggata tcgcagatcg
    17041 aatcaccatt ttacgggatg gacagacgat tgagacacta gaaaatgaaa tgatcagcga
    17101 agagcatatt attcgcggaa tggtgggcag agatttagtt gatcgttatc ctgaaagaaa
    17161 accggatatt ggagaagttt attttaaagt agaagactgg acggtctatc accctcttga
    17221 tcacgagcga atcataaacg accacatcaa cttgcatttg aaaaaaggag agattgtagg
    17281 aatagctgga ctgatgggag cgggcagaac tgaattcgct atgagtctat ttggacattc
    17341 ttatggaagc aagatatccg gacgtatatt aaaggaaggg gcagagatat cagttaagac
    17401 cgttccttca gccattgcaa acggactcgc ttatgtctcg gaggaccgaa aatctttagg
    17461 actgaatctg ctgatggata ttcgggaaaa cgtgacgctg agcagtttgg ataaaataag
    17521 tcaaaaagga gtattagata aagaaaaaga agtaactgag gcagaaaatt tccggaaaaa
    17581 gatgcgtata aaaacaaact ccatccatca aattgtcagt agtcttagtg gtgggaatca
    17641 acaaaaagtc gttttatcca agtggctgat gacacaaccc gatattcttt ttttggatga
    17701 gccaacccgt gggatagatg ttggggcaaa atatgagatc tacacgatta tcgaagaaat
    17761 ggcagcaaat ggcaaagctg tctgtcttat ctcttctgaa cttcctgaaa tattagggat
    17821 gtgtgatcga atctatacga tgaacgaagg acgtattaca ggagaagtct cgcgagaaga
    17881 tgcagatcag gaagtcttga tgaaattaat gacgaaagaa gaggtgggtt aagttggaac
    17941 aggcaaaaaa tcttacggat aaaaaagaga aaaaactaaa tttgaaaagt agagcagctg
    18001 atctttttgg tcgctacagc atggtgatca ttttagcggg actacttatt gcgtttcaat
    18061 tgatgacgga tggtattttc tggcgtccat tgaatataac taaccttgtt ctgcaaaata
    18121 gccatatatt agtactagct gcagggatgt tattagttgt gctcttaggt catgtggatt
    18181 tgtcggtagg gtcagtgatg gcgtttgttg gtgcaattgc gggcatgatg atggtcaatc
    18241 gccaaatgaa cccgtggatg acagttatcg catgtcttgc agtaggcggg ctgatcggcg
    18301 catggcaagg tttttgggta gcttatgcag ggataccggc gtttatcgtg actttagcag
    18361 gattgctgat gtttcgtgga ctgacacaag ttgttctagg aggccaatct ctagcacctt
    18421 ttccagctgt tttccaaaaa atgtctacgg gttatttacc agacttaagc gaaggaagca
    18481 gccttcactt cttgacattg attttaggaa tgatcatcac gatcgtgtta gttgttggtc
    18541 aatggcgaac aagaaatcgt cggaaaaatc atttatttga agtggaatct ccaacagctt
    18601 tcttggtaaa ggcggtattt acagcagtcg ttgtactagg aataacttat atctttgcct
    18661 cttatcaagg gtatccgatt attttgatca ttcttggttt gatcgttgca gcatatgggt
    18721 ttttgacaaa taaaacagta gcaggtcgac agatttatgc gacgggcgga aatcgaaaag
    18781 cggctgaact atcagggatc aaaacaaagc gaatcacgtt ctgggtattc gtcaacatgg
    18841 gagtcatggc agctttagct ggattagttt tagctgctcg attgaatgcg gcaacacctc
    18901 aagcaggtac atcgatggaa ctggatgcaa tggcggcagt atattttggt ggtgcttcga
    18961 cttccggcgg cattggaaca atcatgggtg cgattgtcgg tggcttggtc atgggtgtat
    19021 tgaacaatgg catgtcgatt ttaggcgtcg gggtagactg gcagcaggca atcaaaggat
    19081 tgatcttgct tttagccgtt gtgctagact tgtataataa gaaaaaacga acaacgtagg
    19141 agaacgccgg ttattcagaa aaaagactgt gacaagacat ttgtcacagt ctttttttcg
    19201 ctaaaagcga acattcattg tttcgtttgt atagactaaa aataatatat gtttttttac
    19261 cattcgtata aattctcttt ttgctcttga tttttttgct taattgagtt aaattacata
    19321 gggcgctaaa aaaagccttg attaaattaa taatgttcgt gttttgctgg atgtgaacat
    19381 caggaggata atgtaatgaa agtatttgat tacgaagata ttcaattaat tcctaataaa
    19441 tgtatcgtaa atagccgttc tgaatgtgat acaacggtca cattaggtaa acatacattc
    19501 aaaatgccag tagtgcctgc caacatgcag acgattattg atgaaacaat cgcagaattc
    19561 ttagcggaaa atggttattt ctatatcatg catcggtttg atgaagcagc tcgaattccg
    19621 tttatcaaaa aaatgaaaaa gcgtggattg attacctcga tcagtgttgg cgtcaagaaa
    19681 gaggaatatt cctttataga gaagttggca gaagaatcgc taaatcctga ttatatcact
    19741 atcgacatcg cgcatggaca tgctaactca gttattgata tgattcagca tattaaaaaa
    19801 tatttgcctg aaacttttgt gattgccggt aatgttggta caccggaagc cgtccgtgaa
    19861 ttggaaaatg cgggcgcaga tgcaacaaaa gtcgggattg gtccaggaaa agtatgtatt
    19921 acaaaaatca agacaggctt tgggactgga ggctggcaac tggcagctct tagatggtgt
    19981 gcgaaagcag ctcgcaagcc gatcatcgct gacggtggga tcagaacaca tggcgatatt
    20041 gcaaaatctg tccgttttgg tgcgacaatg gtcatgattg gttcattgtt tgccggtcac
    20101 gaggaatctc ctggcgaaac aaaagtcgaa aatggaatcg tgtataaaga atactttggt
    20161 tctgcttcag aattccaaaa aggcgaaaaa cgaaatgttg aaggaaagaa aatctggatc
    20221 caacacaaag gatctttgaa agatacacta gtggaaatgc aacaagacct tcaatcatct
    20281 atttcttatg ctggaggaag agatctggaa gccattcgaa aagttgacta tgtcatcgtg
    20341 aaaaattcga tttttaatgg agacacaatc taaaaaataa ataaaatatt aggatgttgt
    20401 gaaaaagttg tcctgcatta agtaataaga acagccaaac aaaaatagtt ctttatatgt
    20461 tttgtttggc tggaattatt atcgcagata caagcttttg aacatcatta ttcagaataa
    20521 gggactgtga taagattttt gtcacagtcc cttatgtttt gcaaaaaaag tttttctttt
    20581 ttctcgtaaa ttcggtaaaa tcaaaagaaa aaatagacaa tagaaaagag aagcttatgt
    20641 gggaaaaagt agagaaaaaa caaggaccga tttatcgtca aattatggaa cagattatga
    20701 gagaaatcga aaacggaagg atcaatcctg gaagtcaact tgaatcagaa cgaaaactag
    20761 ggcaactttt tggtgtgaat cgttccaccg ttgtccatgc attggaagag cttagggagc
    20821 ttggggtact aacgagcaaa cgtggcagcg gatggtttgt taatcagact gaatggggga
    20881 aattcgctgt tccaagaatc gattggcggc aaatgatctc tcctcgttat gagcagacgg
    20941 actggtatga gcaaaaaata agagaagcaa agcagatcca aaaaacatcg tttcttgatt
    21001 tatacgctag cgagatgcca gatgagcttt tgccgaattt tgagtttcct tcttattctc
    21061 ttgaagaaat cttagcggaa gaaaaagaaa tggatctttt aggctatact ccgttacgta
    21121 acaaaatcaa acggtatttg gaaagtaaat ttgaattggc attttccccc aaccagcttc
    21181 ttgtgactgg tggcggtcag caagcaatct ttttgatttt acaaacgatt ctttcagctg
    21241 gagaggggat agctgtagag tcaccttctt ttttttaccg attacctctt ttcaaagctt
    21301 caggaacgcg tttatttggg ataccgatgg atgaagaagg gatcgactta gaaaaactga
    21361 aagaatcgat ccataaaaat aaattgaaag cagtgcttat caacccgaat tttcaaaacc
    21421 ctacaggaaa agtgatgagt caaaaacgac gagaaaagct agtagaattg tgtcgacagt
    21481 atcggcttcc aattattgaa gacgatgtat ttacggatct ttcatttcta aaccaagaag
    21541 aagtccttcc tatacgaacg attgatccgg ataatgtgtt atacgtcggt tcgttatcta
    21601 gggttttagg aaaaacaacg aaaatcggtt gggtgatagg acctgagact tttatctcgc
    21661 aactagctca agcgcaagaa atgatggagt tttcgatgaa tatccttaca caaatcacag
    21721 tggctaatgt atttaatgaa gcaattgatt tgaaaatgca agatgtaaga aaaaagttga
    21781 aagaaaaaag ccgactgata agcgcttggg gagattcaca atcgtttttc aagcttcatt
    21841 caattttagg tggttactat gcctggatta cttgggatgg gaaaaaacta gaccccgaac
    21901 tagcagaaaa agtagtagat atgggtcttg gaattgcacc aagtctactt tttgggcaag
    21961 atataaatgg aatcagaatc aattacagta ggctggatga aaaccaaata ccgttgtttt
    22021 attcaaagat gaaaattcta gaaaagtggt taaca
//
LOCUS       DAKBVG010000020        22040 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000020 DAKBVG010000000
VERSION     DAKBVG010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 22040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22040
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.020"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(192..662)
                     /locus_tag="OUD09_000594"
     CDS             complement(192..662)
                     /locus_tag="OUD09_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HCT9691891.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            992..2632
                     /locus_tag="OUD09_000595"
     CDS             992..2632
                     /locus_tag="OUD09_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HCT9691892.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2786..4108
                     /locus_tag="OUD09_000596"
     CDS             2786..4108
                     /locus_tag="OUD09_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HCT9691893.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(4176..4610)
                     /locus_tag="OUD09_000597"
     CDS             complement(4176..4610)
                     /locus_tag="OUD09_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCT9691894.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(4675..5511)
                     /locus_tag="OUD09_000598"
     CDS             complement(4675..5511)
                     /locus_tag="OUD09_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCT9691895.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            complement(6253..6879)
                     /locus_tag="OUD09_000599"
     CDS             complement(6253..6879)
                     /locus_tag="OUD09_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9691896.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(7174..7509)
                     /locus_tag="OUD09_000600"
     CDS             complement(7174..7509)
                     /locus_tag="OUD09_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCT9691897.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(7496..7780)
                     /locus_tag="OUD09_000601"
     CDS             complement(7496..7780)
                     /locus_tag="OUD09_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HCT9691898.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(7836..9359)
                     /locus_tag="OUD09_000602"
     CDS             complement(7836..9359)
                     /locus_tag="OUD09_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCT9691899.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(9352..10527)
                     /locus_tag="OUD09_000603"
     CDS             complement(9352..10527)
                     /locus_tag="OUD09_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCT9691900.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(10531..10716)
                     /locus_tag="OUD09_000604"
     CDS             complement(10531..10716)
                     /locus_tag="OUD09_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9691901.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(10682..12376)
                     /locus_tag="OUD09_000605"
     CDS             complement(10682..12376)
                     /locus_tag="OUD09_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCT9691902.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(12373..12846)
                     /locus_tag="OUD09_000606"
     CDS             complement(12373..12846)
                     /locus_tag="OUD09_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HCT9691903.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(12915..13070)
                     /locus_tag="OUD09_000607"
     CDS             complement(12915..13070)
                     /locus_tag="OUD09_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HCT9691904.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(13204..13584)
                     /locus_tag="OUD09_000608"
     CDS             complement(13204..13584)
                     /locus_tag="OUD09_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCT9691905.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(13588..13803)
                     /locus_tag="OUD09_000609"
     CDS             complement(13588..13803)
                     /locus_tag="OUD09_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9691906.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(13806..14213)
                     /locus_tag="OUD09_000610"
     CDS             complement(13806..14213)
                     /locus_tag="OUD09_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HCT9691907.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(14479..15954)
                     /locus_tag="OUD09_000611"
     CDS             complement(14479..15954)
                     /locus_tag="OUD09_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HCT9691908.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(15944..16792)
                     /locus_tag="OUD09_000612"
     CDS             complement(15944..16792)
                     /locus_tag="OUD09_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HCT9691909.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(16829..17191)
                     /locus_tag="OUD09_000613"
     CDS             complement(16829..17191)
                     /locus_tag="OUD09_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9691910.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(17192..17350)
                     /locus_tag="OUD09_000614"
     CDS             complement(17192..17350)
                     /locus_tag="OUD09_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9691911.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            complement(17491..18115)
                     /locus_tag="OUD09_000615"
                     /pseudo
     CDS             complement(17491..18115)
                     /locus_tag="OUD09_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(18112..18423)
                     /locus_tag="OUD09_000616"
     CDS             complement(18112..18423)
                     /locus_tag="OUD09_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9691912.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(18467..18760)
                     /locus_tag="OUD09_000617"
     CDS             complement(18467..18760)
                     /locus_tag="OUD09_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9691913.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            18954..19601
                     /locus_tag="OUD09_000618"
     CDS             18954..19601
                     /locus_tag="OUD09_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCT9691914.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            19662..20807
                     /locus_tag="OUD09_000619"
     CDS             19662..20807
                     /locus_tag="OUD09_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCT9691915.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(20888..21193)
                     /locus_tag="OUD09_000620"
     CDS             complement(20888..21193)
                     /locus_tag="OUD09_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HCT9691916.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(21218..21649)
                     /gene="ruvX"
                     /locus_tag="OUD09_000621"
     CDS             complement(21218..21649)
                     /gene="ruvX"
                     /locus_tag="OUD09_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCT9691917.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(21646..21912)
                     /locus_tag="OUD09_000622"
     CDS             complement(21646..21912)
                     /locus_tag="OUD09_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HCT9691918.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
BASE COUNT         6392 a         4521 c         3662 g         7465 t
ORIGIN      
        1 tgacttcaca gtctcttcct taaacggtgt tcaaaaactt gcatctgcgg taataagccc
       61 aaccaaacga aaaataggag gagctatttt tgtttggctg ttcttattac ttgatgcagg
      121 gcaggttttt cacgacctct gattcacggt gtttcttttt catcaacatt tttacgaaat
      181 actgtggaag actattgaaa taagtgtgga ttgtttcttt gtgtttcaat cgtttcttgc
      241 cattcttcat ttaattttgg attaccgttt tcttccttga actcataagg tcctaattca
      301 aaaatcccag acagtatttg attcacgatt tcatatagtt cagtgtgatc agacacatga
      361 ttcttgaaga tcatctcatt atcaccacgt ttgaacctca cattcgggct ctcatgatga
      421 acctctatgc atttttctcc tttttttaac tgacggactt ctgaaaacat ctcataaata
      481 tcttctccag taaaaccttc agcaagcgcc agactgtcca caagaccgcc tactgtcagt
      541 tcagagatta ttgcttgatt ggaatctaat tttaatgtat agcttgtcgc ttctactacg
      601 ccacatgcat tgcaaccgcc ttctgatagt tcgctttttc taatcaattg aacttctttc
      661 attttctctt cctcctctta attttctact tatgtatttt tatcacctga cataggtgac
      721 tcctatcaac gctatcatgt gcttttttcc tcagaaatcc tatttatcca ttccatctat
      781 cccttattct tctacttttc tttctctagc atataagaga tacgcatagt attcaagcaa
      841 ggattgtcga tataaaaaga acaaatactg ctattttgat ttcttttggc tatattacat
      901 ataaataaaa tttatcatac ctgctctttt tgactatatc atcatctgac ttctatactt
      961 cgtttgtatt aaaacttagg aggaatcatt catgcttaca gggttaaact tattaaataa
     1021 tccttttcta aataaaggaa cagcttttac aaaagaagaa cgggcaaaat atggaatcac
     1081 tggtatgctt ccaagtactg tccaaacact ggaacaacaa tctgttcaag cttatggtca
     1141 atacttaagc aaacaaacag atttggaaaa acgtatcttc ttaatgaatc tattcaatac
     1201 gaatcggaca ttgttttata aattgatggg cgaacattta gttgaattca tgcctgtcgt
     1261 ttatgatcct gtcgttgcgg attcaatcga acaatacaac gaaattttct tgaaaccgca
     1321 agatgctgca tttctttcaa tcgacgaacc agaaaacatc aaagcaagtt tgaagaacgc
     1381 ggcagatgga cgagacattc gtttgattgt tgtgactgat gcagaaggaa tcttaggtat
     1441 gggcgactgg ggtgtgaacg gtgttgacat cgctatcggc aaattgatgg tctatactgc
     1501 tgctgctggt attaatccag cacaagtttt gcctgtatcg atcgacgctg gtactaataa
     1561 tgaagaatta ttgaataatc ccttatactt aggcaatcgt catgctcgtg tagaaggcga
     1621 aacttactat gaattcatcg atcaattcgt tcaatcagct accgaattat tcccagaatt
     1681 gttattgcat tgggaagatt ttggacgagg aaatgctgca gcgatcttag aaaaatacga
     1741 agatcaaatc actactttca acgacgatat ccaaggtaca ggaattgttg tcctagcagg
     1801 tgtattaggc ggattgaata tatccggtga acaattgaaa gatcaaataa ttttgacatt
     1861 tggtgctggt actgctggtg tgggtatcgc aaacatcctt ttagatgaga tgatccgtca
     1921 aggtgtacct gaagaagaag cacgccaaca tttctatcaa gtagataaac aaggtttatt
     1981 gtttgaagat acagaaggat tgactcctgg tcaaatccca ttcgctcgca aacgctcaga
     2041 tttcactaat gctgatgaat tgacaaactt agaagcagtt gtaaaagcga tccatccgac
     2101 aatcatgatt ggtacttcta cccaacctgg agcattcact gaagaaatcg tgaaagaaat
     2161 ggctgctcat acaccacgtc cgatcatcat gcctttgtct aatccaacaa aattggcaga
     2221 agcaaaagca aaagatttaa tagaatggac ggacggaaaa gcattagttg gaacaggtat
     2281 ccctgctgct gatgttgaat ataatggtgt aacttaccaa atcggtcaag caaataatgc
     2341 tttgatgtac ccaggtttag gacttggttt gattgcttca acagctacac gagtaaatgc
     2401 tgaaatcatt tctcaagcta gtcgagcatt aggcggaatc gttgatgtaa caaaaccagg
     2461 agcagctatc ttgcctccag tagcaaaaat cactgaattt tctcaaacga ttgctgaaac
     2521 tgtcgcaaaa tcagttgtcg cacaaaactt gaatcgtgaa gaaatcacgg atatcaaaga
     2581 agcagttgaa tctgctaaat gggttcctga atataaatct ttagaagatt aagatcgtta
     2641 tagtaaaaga acatacagaa actttgtatg acgtctaaaa agaaaaaagc gactgcggca
     2701 aaacagcggc cagccaactc ctgctctttt gtcgcttttc tttggctttt ttcatatgat
     2761 acagaataag aaaggatctg taaaaatggc acaaaaaaca acaactgcaa ctgcagttga
     2821 ggaaaagaaa aaagaaggtt tttggtccac aaaaatcacc ggcgtaagtt tgccacttta
     2881 tttgatcatg gtccttgtcc tgatcattgc aatggcttta gggaaattac cgacgaatat
     2941 gattggacca atcgcagttt tggttatttt cggtaattta tttcatttta tcgggacaaa
     3001 aatccctatc gtcaaaagtt atttgggtgg tggttccgtt tttgcgatct tcgcttctgc
     3061 tgcgattgct acatttggga tcttacctga atatgtagtg aaatccacag aaaactttgt
     3121 cagcaatatg ggctttttgg acttttatat tgctgccttg attactggta gcatccttgg
     3181 tatgaaccgt aatctattga tgaaagcttc tgttcgtttt attcccgttg cacttatttc
     3241 aatggtcgtc tccttttttg ccgtaggact cgtgggcatg cttatcggaa atggttttgc
     3301 aaattccgtt ttatacatct cattacctgc gatggctggt ggtgttggag ccggagccgt
     3361 tcctttatca acgatttatg caaatgtttt aggtacggat gcttcttcta tcatttcccg
     3421 cctgattcct gcgacagcaa tgacaaatgt cttagctatc atcggtgcag cattagttgc
     3481 tcgtgctgga caatcaatgc caaaattcaa cggcaatggt aaattaatgg aaaaaggcga
     3541 tcttggcgat ggaaaacctc gaactgtgaa accttccatt ccacagttag gtgttggttt
     3601 gatggtatca ttgactttct tcatcttagg acaaattggt aactattttg ttcctaaagt
     3661 ccatgcttac gcatttatga ttattatcgt tgtcatctgt aaaatcgcca acctattacc
     3721 agagtattat gaagatgcag ctatcatgtt caataacttg attgtcaaaa acttgacagc
     3781 agctgttctt gccggtatcg gtattgcgtt gctgaatctg aatgtattag cttctgcttt
     3841 gacatggcaa tttgttgtct tatgtctgac aagtgttatc gcgatttcag ttgtttcagg
     3901 atttgtcggt cgtttgtttg gtttatatcc aattgaatca actattactg ctggcttatg
     3961 caacaactcg atgggcggaa caggaaatgt agccgtctta tctgcagcta atcggatgga
     4021 acttattgct tttgctcaaa tgggcaatcg acttggtggt gccatcgttt tgatcatctc
     4081 tggtttcttg atgcaattac tttcttagtc aaaaaaacaa aaatacctga tcatcaagta
     4141 tcggcttggt gatcgggtat ttttttgata aagatttaca ggattttcaa aaatcttttt
     4201 tgcattttat cggaatgata accgatgtgt tcataaaatt ggtgtgcttc tactctgtat
     4261 tctgcagaat taagccttat tgtcgttatt cctcgttttt tcgcttcttc ttctacgcca
     4321 gtcatcaaag cctttccgat gcctcttttt tcagcatatt tagacacagc aagtgccata
     4381 atattgaaca ttggatcgct aaaaatcgat tcataggact ccgcttgtac atagccagct
     4441 gcttttttag aagtaggatc ttcataaacc aagaaaaaat ttttctctgg ctccttcagc
     4501 agtttcttca tttgtctcac cgtaacggtt aatggtatat cataccctaa ttgatccgca
     4561 ttgatttcac gtagtgcttc tgcatcttct aaacgcattt cccgtaacat ccaaatcacc
     4621 agccaatttc tgatttttta aaatgaattt ttttatcata gtatactctt tttttcattt
     4681 agaacaatat gtttcagtga ttttgacttg cgttagtcta gattctccta atgtttgtaa
     4741 gatgacaaag gcattgtaac aattgaccaa aagaaccacg aggacaatta cttgaaaaat
     4801 aaaggtcact ttttttggag acaaaaccct gctaaatctt gctccccaca aacctccaat
     4861 aacagcagcg gggataacat aaaacaacat agataaatca aagcgcataa agctacttgt
     4921 taaagcaatc gttaccaatt ttgccagttg agaaaagaaa atcgtagata gagaatatag
     4981 tgttgcttct tttattggca atgcaaacat cagcatcaac aaagaaacat tgactggtcc
     5041 accaccaata cctaaaaaac tagctaaaaa gcccagaacc cccccacata taaggtacca
     5101 agtccatgac gtcaaatgaa attttggctg atgatgcttg gtgtagaaga acgcaaaaac
     5161 caaggtcaat accgtcaaaa aaatttggat cagctgcaca tgcttctcgt tttcaaataa
     5221 ctgtaaaaaa acttcgaata caatgttgcc gactactcct ccaacaacag ctccactaga
     5281 tacccaaccg acaatctgcc aattcagtga tttcccgctt gatacttgtc gcacagtcga
     5341 aacaatggac atcgtgaata ccgcaactgt tgagtaaaaa gaaatccctg caacagaatg
     5401 ggcgccgata agatcaagaa tcggtttgat caacacgcct ccacccatcc cggaaatagc
     5461 gccaactgtg ttcgccagaa cgatcaccac aaaataaatc agaccaatca tcttgtattt
     5521 cctttctctt gtttatatct tttagcgatt gttactcttt tcttttatta tacagtaaaa
     5581 gaaacaaacg aggcttcgaa agcttttcta ctttttaagg aaatttagtg ctaaacgaaa
     5641 agaggttgtg aaaaagctgt cctgcatcaa gtaataagaa cagccaaaca aaaatagctc
     5701 ctcataattt tcgtttggtt gaacttatta ccgcagatgc aagcttttga acaccgttta
     5761 agaaagaggt cgtgagcctg ccgttcagct ccgagtcaca aggaacaatt tcaaaaaaca
     5821 gcttctcatg tttgttgaat attgtgactt gtgaccgagg agttggcagg agaacaccgt
     5881 ttaaggaaga gactgtgaag tcaacgtttt gacctgatta ttagagaaac aaatggcaaa
     5941 atagttcttc atatttcgcc gtttttcgag cgaatgccga ggggcagctg attgaacatc
     6001 gtttatttgg aaaaagggat tgtgacaaga cgtttgtcac aatccctttt tttatatctt
     6061 attaattcat tcttttgttc tattcttctt cttccgccat gaatgagaaa cccatatcaa
     6121 agaactataa aggttatcaa aacagcgttt atgactaaat gaattagagt tatctatcac
     6181 tatttttcaa gcgatttaaa ccaaatggca tacaatatgg catacaaaaa cttcaataag
     6241 ccgtgggggt ttttaaattt cttcttgttc tttaaaatca atgagtggag ctacataatt
     6301 tgaatccact ttttcaaacg tcactttttg catcgacgaa tgttttcttt cccaagataa
     6361 cgtaaataga tttgactctg gacttataaa gtctacttca ccttctagta ttactttctt
     6421 atgcaaatat tcctctaata aatatagact tgctaaagca tacaacacat tttccaaatt
     6481 tgcttcctta taatttgaag aacgaccatg ttttactctg ttatatattt tccaccaatc
     6541 tggtgtatct tcaggccacc catctccaaa aggttttagt gaagtcaatg ttaaagaatt
     6601 cataacccta acaccattat ccaaacacat taattcgcta gatttaataa cagctttaaa
     6661 atcagttatg cttttattgc tactgctatc aaatccgcaa agttctttga aaataatctc
     6721 acactccgaa ccaatcgcta gtagtagttt ttgatattca atagagaatg ttttgaaatt
     6781 attctcatcg agcatcacaa aatctgtggt attaataaac tcattttcta aatgtagata
     6841 atacttccaa taaaaatttg taaactctgc tttgtccatt aaatcacctc ttttgaatta
     6901 taacaaaaaa aacacgtcca caacagacgt gttctttgat ttctacacaa tcagcttgcc
     6961 aaaaccatcc aatcagtcct agcatgcctg tattaaaatt gaacgttcat catctatcat
     7021 ctttcttagt ccagcatgtt tgcaaccaca cctacaattt tcagtccaac aatagatttc
     7081 gactagctaa gtcttgcaaa attcaggcag ctaacccttc atttactacc ttaattatac
     7141 taaataccca caacgtttac aagtgtaatc attctaagaa atctgtttag ctatgatcgt
     7201 tgtataatct ttaaacgccg ttccttcatt gattgaaata attttataac tcttgttttt
     7261 ccaagctact ttcatatcgt ttgttagttc ataatcttga tcgtatcgaa taataaacgt
     7321 caacgttcct tcaagaatgg tacctacact agcaagcacg tcttttagca tttgtgaacg
     7381 cacattagcc caacattcaa atacagtttc ttcactatcc accatctgcc catcttcatc
     7441 ttttacccgt tttgtctttt taaacttgac tctctcagtc aattcattta cattcttaat
     7501 gattggcata ataagcacct ctcatttgtt gtactaatgc ttgtaccgtt actggtatcc
     7561 gttcgctgct ggcttcttga cggttcaaat accaatgttg agtaagtaat gacaccgcca
     7621 aatcaaattg ctgataatta tccatatctt tatccgttgc gctactatca atagcacttt
     7681 ttatatattc gcctgctgta tcaatcaatt gttgaataaa gtcatcatca atagtgtgat
     7741 cgatccgcat actttttttt atgctatcca aaattaccat tttatctctc ctaatgaaaa
     7801 aaaggcagtg ggataaaccg ccgcccaata tttttctata cttttggtgt tacaactggc
     7861 gtaaactcaa tatacactga agcattttga tcaattttct tataatcgtt gcgaacgatc
     7921 actgcaagtc cttgtgagta gtaatcgaat ttttcccatt gagtcgttac ttgatttcta
     7981 cgagcaacaa agatagattg tgcaatatcc cccataatca tagggaatgt tccagcttta
     8041 ggattggcaa acaatgtatc tgcaatcaat actactggca taccgaataa tgatttacca
     8101 ctaggtgctg tcacatcggg ttgtaaaatg taacgtccat ctgaatcttt caatgtatct
     8161 aggtggtaat atccgctttg gtttagaatt accattttat ttaatgctgg gtctaatgcc
     8221 acattgtata gttgtttcaa atcgtccaac gtagcagccg ttttcttagt gaatgttttt
     8281 aataaatcca tgatatgctt attgtcagtg ttttctacca atttcgctaa ctgatctttg
     8341 acctcttgta caatattcac tgctgaatct tccacaacct catttgataa ggcaatctta
     8401 ccagcacgag tttctacttt ataatcaact gaagtaaaca tttcagcgtc aatatcacca
     8461 atttcagcaa gttcagcttt tgttgctaac actgcttgtt ggttagttgc tactggatat
     8521 ttaccttgtc catttgatac tgtttttact gttgcatatt gagctaagtt ataatttgaa
     8581 cgtttcaaat caaagacttc accgattact tcttcgggaa caactgccgc tgcatttaca
     8641 gtagtgactc catcacgcac ttcgccttgt gaacggatat attcttcata cccacgcaat
     8701 tctgtttttt cgttatcgat taaagttttt tccatgttat tactctcctt ttgtgcttta
     8761 gaagtggatt ctaagctatt gtttgtttgt tttgcttggt tgttactcat aaacgattcg
     8821 taagaacgct tatttacttg gacatttgat gaatcataag cgggaacggt cacgacacta
     8881 atttcattaa gtgctttgat tttatctatt gatcgttcaa ttgtgccatc ttcttttttg
     8941 tcgaatgaat ctaatccaag cacgaaccca aaggacatag aatcaacaac cccttttgag
     9001 atattctcat acacatcatt tgaataggtc gtgtcggtta gctctgcttc aaaatgtaat
     9061 cctgtatcat cgacattcaa tttcaacgta cctgctttga cactagctag tggctttgaa
     9121 taatcgtgtc catataataa gaacacattt gataaatcca cctcttttaa tgcttcgggt
     9181 gtaatcactt ctacaaagcc gcctaaatct tttgacggtt gcccgaattt caacgcatag
     9241 cctgaaatag ttttgccgtc tgttggttgt ttttcatttt ctttctctac tttgggagaa
     9301 tcggctgtta gctcagcttc ttctgtcagc cgtttttcct tttcttcttc attattcaac
     9361 tggtgtaact ccttcctgtt cttttaattg atatttctcc aacgtatcta aatacgtaaa
     9421 gttcaaactt gctagtaaac gatcgccacc atccatgggg ggtagcccca ttttcgaccg
     9481 cccctcatta atcgtgagaa gtgacccttt gacctgatcc aatacatttt ttacttttgt
     9541 ttcaggatca gtctctagta actgatcgga attaaagcgg atattggttt caagtttcgt
     9601 atctaattca ctcacaaaca cattaaagta atgaatcagc gtactttgga tatactggag
     9661 attactttga acggtgctag aatgttcatt ttcaacgcct aaacgctcaa tcggtacacc
     9721 aaacgccttg gcaatttgtt ttgtgttcca atcattggaa ttgataagct tcaaaacatc
     9781 ggtgtttatt tctaatgttt tgtagtccat tgtttcatca agaatgatcg ttctaagagc
     9841 attatctcca ctacttgaac cgttggcttc ttcaaacttt tcacgaattg cgttctttgc
     9901 tgatccgtct aaatctgact tatgaacctt caaaatacct gacccactga caccacgagt
     9961 aaagaaattg tgcaatgtgc ggttgccagc ttgttgaatt ttcagttcat cacgtaacgc
    10021 ataaagtggt ggtaatcctg ttaaaccatc ttgggtgaaa tatttgaagt gcaaaatatc
    10081 gctagacttc acacgtcttt ttttatcacc aatctgataa gacaacgtac cattatctaa
    10141 ttgagtaacc gttacacttg agttaggtaa taagtgaatt tcttctacct tttcaccgtt
    10201 gcgtttaatc tctgcaaagc tattaccgtt tagcaacata ttgaccgcca aagcaaactt
    10261 taaatgccaa ccatccattt ctgaattagg tttctcgttt agtaacttca ccagttcatt
    10321 atcagcttgt ggcatattgt ttttgaccaa ttgaatcgga cttgaagcaa tatcactagc
    10381 aataattcga acggctgcaa acacatcact atttcttaat gcacgaacgc ttgtaaagct
    10441 ggtggtgtaa tcatccgatt ggattgatac cacatgttgt aggaaaggat ctcccgttgt
    10501 attttgtctc gctttaaaaa atgccatctt ttaacctccc ttctgttctg tatttagcat
    10561 gaccgctacc ccaataagca ctatgcccag cgctaacaag ccatagtaga catttgttag
    10621 taaggtgata gcaaccaaca cacacaccaa tccaagaagt aaaagaacgg tatgaatgta
    10681 attagaaaga aaattcttca cttgtataaa attcattgtc tgctttcgct ccttccgttg
    10741 tggtgaagta atccattgca aacacatagg cattgattaa tgcagctatt ggatcaattt
    10801 tgttactgtt ctttgattta ttaatctgaa tgccgttgtt atccgttttt aaaatagcgt
    10861 tgttcactgc atgggtgaga atcgtatttt tattgtgaat aacgttgcct tcataaactt
    10921 gttctctaaa agttcgggtc ggaacgttta gagtaatcgt tccttgtctt acttctaaca
    10981 ttgggtagtt ggctttttca gctttactga taagcgaatt agcgttatac gggtcataac
    11041 aaatccccat acattccaaa tcattttctt gaatcaaatc ttgaataaac tgaaatactt
    11101 ggtcatagtc cacgattcca ctttctaatt gagtaatgga acactcacca gctttttcta
    11161 gttctcgata aggcaaaccg tcgcgctttt ctttgtcttg aagcccatat ttggtggcta
    11221 caaagctatg tgaatcacaa taaagttcgc cgttatctag cggtacgatc catgaaacgc
    11281 tagttaaatc atctgtttta gataaatcca ctccgatata aacgggcttg cctgtaatat
    11341 cttgtggttc tacttcggtt gcctgccagt cttcagcaat catatagctg tcctcactcg
    11401 cttgtctcca taaattgaag tttttcacca atacagaatt aaggttattt tgtttcaaag
    11461 caaggttcac atcatcttga atggttggaa tcattacttt tttgatttct tcactttcaa
    11521 aaatcggatt ggctttgatc catttctctt gatcgtgaat ttcttcggga tcgtccaatt
    11581 cccatatcgc tataaagtac ctgtcggctt gttcttcgcc ttttagcaca cgatctaaca
    11641 ataagtattc ctcgtacata ggaacgttta aatcaagccc agcggtacta ataacacaca
    11701 ataagccgtt cttttgttgg gtcattcctg acttaatgac gttgtacgtt ttacgagttt
    11761 ttgcttcgtg ccactcgtca agaattgcgg ttgttggtgc aaacccatct agcgtgctgg
    11821 tttcactagc cagtgccata gcgaacgaat tagagggcaa gtgctgaatc ttagagttca
    11881 taattttcag ttgtggtctt aaaaacttac tagacttgac cacgtttcta agtgaattag
    11941 acagcatatc atagcccaat ttagcttgtt tcaaagcgtt acttacaaac aatacttgcc
    12001 ttgcttctgc tggttcttgt tccataatca acgcattggc agccatgcca cttgcgatat
    12061 acgttttccc attcttacgg ctcatactgg taaacgctcg attgaatcgc ctgtaattcc
    12121 cttctttagt acgccagcca taaagactgc ctagaatgaa tttttgaaat cctaacatct
    12181 tgattgcttt accgtccgta gatggtagca actcaatgaa tttaatcgct ttggtggctt
    12241 gttcttcatc gaacacataa gggaagctat ctgacttaga acgctccaaa tcgcttatat
    12301 gacgctcaca cgcttggata attttcttgg gtgcaatcag cttacctgat aggactttct
    12361 caatgtaatc attcatgaat tcatcaactc tttgaacggg tcttcgggtt catcattttt
    12421 aaccttgttc aaagctagtt ttgatcgtga ttctaaggtc atacctagtg aggtggcttg
    12481 ttttttcaaa tcactcatag cttgtgattg aacccgaacc gctggattgg ctttctttac
    12541 tccgttttcg catgtgataa acgtacctga ttttcttatc tcggcttcgg ctgttttcat
    12601 tcgagcaaag gctaaacaat aggctaccaa tgaaccataa tctgcttcac tcaatggata
    12661 atcttttttt aatgttggta cgatacgatt ccattcactt ctagcgctcg ctgccatcca
    12721 gtcagggggc gtgatattta ttaatggttg ctgcttgaac aattcttctc tagcgtccac
    12781 acggattgct ttttcttcac ttgatatatt gccttttgta tcgtgtaata ttttagggtg
    12841 tcccatgttg tcctcctttc agacaaaaga aaaaggaacg taagcaatta agctcacatt
    12901 ccttcgtttt tcgatcagaa tttttcttta tctaatttat ctatataaat gatttttccg
    12961 ttctgcattt gaatggtcac ttgaccaaaa tgaggtaact caatggtgcg ctgtttccct
    13021 gttttatcag taataacaat tgtatttttc aataaatctc ctactttcat tatatacttt
    13081 ttcacttgtg attacaagtg taatcgttat ttgtaattac ccttatgcct tttcacaacg
    13141 gttatttgta ccaaaatgag taggcatcga ttgtcagtaa gtttcacact atgccccctt
    13201 aactcaaagg gtactctttt ctcgtctttg tctttcattg gctgtcttgg aattatggtg
    13261 tgcatggcat aaagaacgaa aattagtttc atctaaccgt ctattccaat catcttttag
    13321 ttcaattttg tgatctacaa catcagcttt ccttatcaag ccttcttcta ggcaatcctc
    13381 acatactgga tgatttaacc gatacaagta tgaagccttt cgccatgttc tactttgata
    13441 gaattgaaaa tatttacctt cgctttgctt tctttcttca cgcttaattg ccgtttgctt
    13501 tgctttatgc ttcccacaat acttctgcct ataatcaatc aatgttttac atccagcatg
    13561 attacattgt tttttaactg ccatagatta ccaaccaaag cgatctcgtt cgtcagaatc
    13621 atcttgttcc cattttgatt tgtattcaat ataaggcgaa actatcttca agcacgttcc
    13681 gtctttaaaa ttaactttag tgattttatc ttcctcaaaa ctaataaagt ctacatcttc
    13741 ttgagtgttt gaatactcaa tgtgtttctc accatcgaag tatctaatac tttccatatg
    13801 cataattatt ctacctccaa taaattagtt atcaattcat catctgttag tagcaaagcc
    13861 aagaacttat catggatgtt taaaaacaaa ttatcctcat tcgatgtaag ctctcgttgt
    13921 ctcgctcgat catataggtt aagcagacaa ttaaattcat ccattagctc acctgtatca
    13981 tcatagttgc cactatctac caatgatccc actactagcg attgacacca cgctactgac
    14041 tcctgcagcc aatcaggttc ttcttccatt atctttaata cttctggatc aaatgtggaa
    14101 taatcttctg ttaccaatcc cacactaaat gccagcgtgt tcattagaaa ccgatccatt
    14161 tcatctgtat attgaaagtt cggtgctttg tttgttgaaa taatattgag catttacttt
    14221 cctccttgat tcgtaacacg tggtaacacc tacgtaacag gtacccgtta ccactcaatc
    14281 ccaacgggga caagggttct agcgttttgg taacaggtaa cgggtaaaat ccagtaacat
    14341 tattccccag catattagct aactatttca aaagttttaa ggaaataagg tgttaccccg
    14401 ttaccaaatt gctgtatccc ttggggcagt aagggtaaac tagtaacggg ctaggtgtta
    14461 ctgacccgtt actacccgtt actttttgta ctttccagct cgatttttat tttcataggc
    14521 atttgactta ttgtaaaatc ctcgtcgttt atttcttttt ggcagttgct tccgttccca
    14581 accaggaata ctgctaataa tcaaggcaat ctttttagtt tctgcatctg atcgaccatt
    14641 caataaatcc tttgcttgtt tatcaaaaac agcttccaaa atatcagcgg tgagtacgct
    14701 atcaactaaa taaaactctt gttgcatagt agctccatta tagaaatgct gtaaattttt
    14761 ctcattacat ttactgtcag gatagttctg ataataagaa cgtctcgccc acgctggagc
    14821 ttcttcccat ttatacggga tttccatttc caaatattta gtgattgctt ctttaattgg
    14881 attctccatc ttggcatcct cttgatagtc ttttgcaatt tctaagactt ctttatcttc
    14941 atcgggacta aaatagattc gttgtccttt atcaaaaagc acctttgctt cagctaacac
    15001 ttgaagaaaa tattcatcct ttgttttaaa cggattcatc tttggcttat tcttacaagg
    15061 caaaggataa aaacggcggt ttcctgtatc atctttcaaa tactgcccat cattcgacgt
    15121 tccaataaat acacattgcc ttgcccaagc tatgacattc cgaccataag gaggtctaat
    15181 tttatcgatc ttggcactga tgaagttttt tatctgttca attttagttt tgttcattga
    15241 agataattct ccaagttcaa taattacatt gccttgaagc tgcatataat catctttctt
    15301 ttcacctagc gaatctaatg aatcggtgaa aaaatcatcc gtgtaaagta atgctggaac
    15361 ggtactctta ccaatccctt gcggtccatc caaaactgga acgatctcaa actttattcc
    15421 tggtttatag atacgtgcga ctgatccgac tatccaacgt tttgttactt ctcgtgtgta
    15481 aaggttgtcg tctaccccta aaaggtcaat aaacagtgtt tccagtctag gctgttgatc
    15541 ccatttaact ttttcgatac gttcttttac tgggttgaaa acattctttc tagccacatt
    15601 tactactgct ttgtgtattt tgttgtcatc aaaaactaca ttgtagtttt cttcaatata
    15661 tgccgttaag gcatcctcaa aactgtcttc ccattctcca gcggtacgat tgatccaagg
    15721 agaatctttt aataggtgaa tgtatccgct aaattcgtta taagcaattg ttccagctaa
    15781 attgctatca ttggttaaaa tcaattctgc attcacaaca ctgttacttt taatctgatc
    15841 gtctttattg catcttaatc gtgacatcca ggttttcaat tcaatgatat tgtcttcacg
    15901 ctcgctggct tcgggaaagt ctaaaatact atctaattta tgcttatgag ccatgagctt
    15961 tccccctcaa atctttttta atcatcgatc gaataatttg attcacttct ttatctttta
    16021 gcggtggttc attgtacgca tttgctgtat aaatcaattc tgctaaagtt tgattatcta
    16081 caccgtgcca tagtaaccaa ccgcataact tagttaaatg atcgtttcta ctaccttcgc
    16141 cagcgccttg aacaatcata tctaattttt ttgctgtccc tgtttttgct acgggtcggt
    16201 attgagtctt tatttcttgc ggttttttta gcagtaggtt cactagccaa tcgggagctg
    16261 gtgcaatatt atttagagat acgttttcaa taggttcata tgctctccca acacttcttg
    16321 aaataatcat agttggcgct attggaactc ctttagtgat aatatcaatc ccgcttaaac
    16381 ttttaccttt atcatcacgc ccgaagatat cagcaaatgc acctgtttct tgttggataa
    16441 ttacaccatc aggaatttta aagaagaaat ggattccttt acctggtgtg atttccgtat
    16501 aagtagaagg aaatggatca tacattgaag caagtttttt ataattctca acgccgtttc
    16561 gtgtttcaga atgttgatct aggtcaacta ccaccatttg atgatcttca agcattaacc
    16621 caatatttct agttgcatct tccgtccacc attcattaat ttgattagaa tcgttagttg
    16681 catcattcca accactagag ccttttattg gaattttagt tccaggagat aagggataaa
    16741 ttgaccatcc cgacttttgt aattccgttg ctgcttgata attatttgcc aataaacctc
    16801 tcctcctttt tcaaaatact ggtataaatc attcagacac ctccatgagc ttcaactgct
    16861 caagttcgta tttcaaccca tcccgaacaa attcagcacc ttttaattga ttctgatact
    16921 ttacccatgt attaaatgtt ttcacccttg cttcttgcat atcagtaatg tttaaccatg
    16981 cggttaaaat ctgatttttt agctcgttga tttctcgaca aattattttg tgttctgcat
    17041 gttttttgag tacatacaaa actgttagtt tttcattgaa tgaatttcca aagtaggttt
    17101 tcccgatatt attttctagt tcagcgatcc cttgattaat ttcctctaat tcaaggctat
    17161 cgagttggtt taatagtttt gttttgtaca tctacttatg ccctccagtt cttaattgat
    17221 agtcgtactc gtcataagca gtgaagagat ataacgctaa tcctgcaaag atagctattt
    17281 ttgcaattaa tggtaataag ccgaaaattc cgccgattaa aatgcttcct ataattaagt
    17341 ttcgtttcat aatggttccc tccgattttt taatatggta taatcggaac aaagaatatc
    17401 gggttttatc ccaattaagt tgcctttatc ctgtccgcca agactgttat ggataaggct
    17461 ttttctttgt tctcgatcat gttaatgctc ctaagttgtc tgcaattcag cagaacgatt
    17521 ataaattttc tcactcactg aaaactgcaa cccatatttt tctttaacag tcattaattc
    17581 tactgtctca tctaaaatag gttcacgatc aaccaacatt tcaggagtca tatctttctt
    17641 ttttaccatt ttggaatgac cataaatagt tgatacagct ttattggcta tcacattcgc
    17701 tttaatgtag tctactggtt ttggttcagt aatagcgtgg cttaatttag tcattgcttc
    17761 tttttggtgt tctttgtcta gcatgcggaa tacttgaaag ccttcaagtc ctgtggactg
    17821 gcgtagttct ttgatgatgt cgaaaatcca gtcttgaaat gcctcggctt cttttttacg
    17881 gcttttgaat gccatgcggt aaatattttt tttcgttgat gatgtttaca tcttgtgttc
    17941 tgcccaatga atcgatgacc ttactagtag taaccccatc tttaggcaaa gagtgaatgg
    18001 ctctagtaac ctgtttaaga cctaacgctt ccgcaacatc tttcgccact gcccaccatt
    18061 catcattaat atcgacaaat cttataatgt gtccgttcca aatttcagtt ttcattcaac
    18121 gtcctccagt tcaagcccta gcaagctcgc taacaactca acttgctgat acaaaatttc
    18181 ttgatactct tcaatagaaa gtcgttcttt aaactctgct tgtactaaat cgttcaagcc
    18241 acgcaatact ccgcttaccg ttggttcttg tggtagcaat ttttgatagt ccatttgttc
    18301 gtacaaacat tcaaacgttt cttctagcgc tttgccttcc aagtttgcta attctgccgc
    18361 tgtgtcttct gtaagaattg ttgataatac tttttcgatt gctttatttt gtgtcgattc
    18421 cattattatt tcctcctatt aatccctttg cccccacggc ttgcgtttat ttttggtatt
    18481 cttccataaa gcgatcacat tcatcttgtg aaattcttac gactccatca actataatca
    18541 actttaaacc gttaggaaca tatttctgca tcatagtatt gtatgaaacg ttcatatact
    18601 tagcagcttg tgccttgttc atatattttg gaaactggta ctttttttct aataatgaaa
    18661 gtaacgttcc ttgagtttgc tctacttgtt gaacaactcg atcgtcaata attttaaaag
    18721 ctggatcatc aagcgtgagt ttgtcaattt ttaattccat atttatcccc cctcgttagc
    18781 atacatttct atacaaagat tttaaaaaaa tgcaagaggc tcttgaatac atttctatac
    18841 ctcttataaa ctcaatatac catcatacct tttcatttgc aagaatttta acatactttt
    18901 ttgaactttt atgtttcaca catatttaaa tcgtgatata gtagagaaaa tatatgaatg
    18961 gtggtgaaaa tgtggctaga aaaaaatacc ccgaagcagt ggagacagga caaagaatta
    19021 gactggctag aattgaaaag ggactctcaa tggaacaact tggaggtaaa ttatccccac
    19081 cggccagtaa aggtgccgtt agtaattggg aaaatggcta taatcttccc aataacacca
    19141 ggcttaaaca actttcaaag gtattagatg tatccactac ctatctatta aacggatctt
    19201 atacattaag agatatgcat atgatgcccg aaattaagcg aaatgtgctt gaaaagcaat
    19261 tgaaaaaaac tattgaaaaa aataactcta taattgattc taatctttct aatgctatca
    19321 ataacatcga agaagataac ctgactgtct cacagaagat ttatttaaca aacgcaataa
    19381 aatatcttaa aacaggatct cgaattgaat ttcctgacgg gagtacccca attgcttatc
    19441 tcgcagctct tctaaatacg cttttacgat atgactcatt aattaatgat tcagaaaaaa
    19501 atacagatga gaaaaaagag tatcaagaat tttatgaaaa tgatcttcta aaagagttca
    19561 cagctcttgt tactgagcta aataaacact attcaaaata ataataaact gcctcctcca
    19621 accgccccca cggcttgcta cactatggag ggaaaactat tatggcaaca ttcaagcaat
    19681 atgaaactaa aaaaggtaaa ttttggctct atgaagccta tcttggaatg aacaaaatga
    19741 ctggcaaacc tgataaggtt cgtagacgag gttacaaaac aaaaaaagaa gcccagcttg
    19801 ccttgtcacg cttgcaagtg gactacgata aaaacggtat taaaaaagcg aacaacgaaa
    19861 cgtttaaaga agtttatgac ttgtggctag aaacatataa aaccactgtc cgagaagcca
    19921 ccttcatgaa aactgaaatc aagtttagaa agtggattct tccaaaatat ggaaatcttc
    19981 gtgtgaatga agtcacggta aaaaaagccc aacaaattgt aaatagctgg gctgaaacta
    20041 ctgaccaata caaggtactt cattcaactt ctaagcgtat ttttagctac gctatcaatc
    20101 ttgggattat tgagactaat ccactagaca acgtattaat gcctaaaaga accaccacta
    20161 agcataaaga aaaggtcaaa gtttacagta aagaacaact gatcaccttg tttgattacc
    20221 taaacagcaa acctgaaact tatcgaaacg actttgataa aacgttacta cgctttctct
    20281 tttatagcgg ggttaggatc agtgaagccc ttgcattgaa ttggtcagac attgattttc
    20341 aagaaaatac ggtcaccatc aataaaacac taagccaatc caaaaatggc tataaaatca
    20401 gtgatcctaa aactgattgt agtgctgggg ttcttccact agatgaaaga acaatttcta
    20461 tattaaaaaa atggcagatt aatcagcgga aatatatgct gacacttggt gtaactgatc
    20521 cgactatgat tttttgtggt atctataaag aactaatcac acaccacgct atttatgcaa
    20581 gattgattac tatttgtgaa aaagctatgg ttccatttct cggtgtgcat gtagcaagac
    20641 acactcatgc ttcaatgctc ttggacgctg gagctactat ggtagaagtt caaactagat
    20701 tacggcatac aaaaattagt cagacgatcg attgttacgg tcacttggca aaagaaacga
    20761 aagaaaagac tgttgaaaaa ttagtacaac acttaaatat tgactagatg gcatacaata
    20821 tggcatacag taaaaaataa aaaaaggaga aaccctatca tatcaaggtt tctcctagct
    20881 ttatagtcta ttcttcttct tccgccatga atgtattgaa tacttcttca atcatgtccc
    20941 attctgcgtc tgtttcgatt tgttctaact cgccttctgt tccatcttcg ttttcgatgt
    21001 atgcataagc ttgtaattct acatcttcat cttctgaaac accagcagga tataaaagaa
    21061 catagttacg tccaaattct tcttgtccgt caactgttag taggatttca tataatgttt
    21121 catttccttg gtcatccacc aatgtgatgt gttcgtgtcc ttcatggtcg tgggaatggt
    21181 tatgttctgt cataagatta cctcttttct tttattatta aaataattgc ttacctgagc
    21241 cgtctaaata attttgtaag atcatcacag ctgctaattt atcgatgact ttttttcgtt
    21301 tggctcttga agtatttgcc tgctctacta gcatacgttc tgcctgaaca gtggttaatc
    21361 gttcatcttg ataatcaact ggcaaggaga atagttcttt gatcttttct ccgtatgaca
    21421 tggaagcttc tgcccttggt ccgatggaat tgttcatatt tttaggtaat ccaactacaa
    21481 accggtctac ttcatattgt gctactaatt ccttcaaccg atcaaagcca aactcttctt
    21541 ctgcttcatt gatccgaatg atctctactc cttgagctgt ccagccaaag gggtcactga
    21601 tagcaacacc tactgtctta gaaccgacat ccaagcccat tactctcata aattcactcc
    21661 atgatttgag aggtagtcct tgacaattac ttccatgatc tcatcgcgtt cgtgacggcg
    21721 aatcaaattt cgagcatcac gataacgagg aatgtaggcc gggtctccag aaagcaagta
    21781 gccgacaatc tgattgattg gattgtagcc tttttcttcc aaagcccgat aaacaatcgc
    21841 taatgtttcg ctcacttctt ttttgcgact atcgtcaaaa ccaaaacgta cggtttcatc
    21901 tgtaaaaccc atgattctac acctctttcc cgcaaggtaa ttccagctta ccttctacaa
    21961 ccttatttta ctcgaagaaa gggaaaacta caaacaaata ttccaaactt ccgaataaat
    22021 taagaaacac tatttgtatg
//
LOCUS       DAKBVG010000021        21979 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000021 DAKBVG010000000
VERSION     DAKBVG010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 21979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.021"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            156..1862
                     /gene="efbA"
                     /locus_tag="OUD09_000623"
     CDS             156..1862
                     /gene="efbA"
                     /locus_tag="OUD09_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HCT9691919.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2020..2922)
                     /locus_tag="OUD09_000624"
     CDS             complement(2020..2922)
                     /locus_tag="OUD09_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCT9691920.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2919..3611)
                     /locus_tag="OUD09_000625"
     CDS             complement(2919..3611)
                     /locus_tag="OUD09_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HCT9691921.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3654..4295)
                     /locus_tag="OUD09_000626"
     CDS             complement(3654..4295)
                     /locus_tag="OUD09_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCT9691922.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4333..4647)
                     /locus_tag="OUD09_000627"
     CDS             complement(4333..4647)
                     /locus_tag="OUD09_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9691923.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4635..5987)
                     /locus_tag="OUD09_000628"
                     /pseudo
     CDS             complement(4635..5987)
                     /locus_tag="OUD09_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372436.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
     gene            complement(5984..6916)
                     /locus_tag="OUD09_000629"
     CDS             complement(5984..6916)
                     /locus_tag="OUD09_000629"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HCT9691924.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6913..7689)
                     /locus_tag="OUD09_000630"
     CDS             complement(6913..7689)
                     /locus_tag="OUD09_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HCT9691925.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7743..10928)
                     /gene="carB"
                     /locus_tag="OUD09_000631"
     CDS             complement(7743..10928)
                     /gene="carB"
                     /locus_tag="OUD09_000631"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCT9691926.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10933..12009)
                     /gene="carA"
                     /locus_tag="OUD09_000632"
     CDS             complement(10933..12009)
                     /gene="carA"
                     /locus_tag="OUD09_000632"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCT9691927.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12014..13294)
                     /locus_tag="OUD09_000633"
     CDS             complement(12014..13294)
                     /locus_tag="OUD09_000633"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCT9691928.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13313..14239)
                     /locus_tag="OUD09_000634"
     CDS             complement(13313..14239)
                     /locus_tag="OUD09_000634"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HCT9691929.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14252..15535)
                     /locus_tag="OUD09_000635"
     CDS             complement(14252..15535)
                     /locus_tag="OUD09_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCT9691930.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="OUD09_000636"
     CDS             complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="OUD09_000636"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HCT9691931.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16323..17234)
                     /locus_tag="OUD09_000637"
     CDS             complement(16323..17234)
                     /locus_tag="OUD09_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCT9691932.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17231..17695)
                     /locus_tag="OUD09_000638"
     CDS             complement(17231..17695)
                     /locus_tag="OUD09_000638"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCT9691933.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17700..18398)
                     /locus_tag="OUD09_000639"
     CDS             complement(17700..18398)
                     /locus_tag="OUD09_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCT9691934.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18385..18771)
                     /locus_tag="OUD09_000640"
     CDS             complement(18385..18771)
                     /locus_tag="OUD09_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9691935.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18918..20588)
                     /locus_tag="OUD09_000641"
     CDS             complement(18918..20588)
                     /locus_tag="OUD09_000641"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HCT9691936.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20771..21004
                     /locus_tag="OUD09_000642"
     CDS             20771..21004
                     /locus_tag="OUD09_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HCT9691937.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21103..21534)
                     /locus_tag="OUD09_000643"
     CDS             complement(21103..21534)
                     /locus_tag="OUD09_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HCT9691938.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21613..>21979
                     /locus_tag="OUD09_000644"
     CDS             21613..>21979
                     /locus_tag="OUD09_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HCT9691939.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNL"
BASE COUNT         6207 a         4915 c         3885 g         6972 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaaattctg tcacaacccc tcttttttga
     1981 tcaacggtgt tcaataaaca gcgttctcat ctgcttttct tagctctgct ggtccagtaa
     2041 ccgagtaagc tctttgataa atctttgagc gatttcactg agcggacgtt tcgccgaata
     2101 aatgatataa aattcattta gttcgccttc tgccaaaggg attaaccgca agtgctcgcg
     2161 aatccctcca atcacaatac cagatcctga agcgtaagca tcagtttcaa gaacgatatt
     2221 catcaaagta cctcgatcac tcgtataaat cactgtttct tgatcaggga tctcgataag
     2281 gtcttcataa aaatacggga aattatttcc atcctgtgta aaacgaactt gaggataaga
     2341 taacaattct tctttggtga tctctgcgtg ttcagctaat ggatgtcctt gccttaaaaa
     2401 aatatgcgtt ttgaattttc caatgctgcg gccacttagc tctccttgtt gtaaatatct
     2461 tctgatacca gcttgattat gctcattcat ataaataatg ccgatctcac tgtgctgctc
     2521 tttgacttct tcgatcactc tcgctgttgt cgtctccatg atccgaaatt gtttgatctc
     2581 gtctttgaat tcctcgagta gctgtgcggt caaaggacct aagaaatcat aatgctgggc
     2641 agcgatcgaa aaattgtctc tcttttcact cgttagatag tgagtttcta atgtatcaag
     2701 ctggaccaat attttctcgg cacgatttaa gaagtcttgc ccttcttctg ttaaatatgc
     2761 ccctcgattc gttcgagtaa acagcgtgat ccctaactcg gtttctaatt ctttgatacc
     2821 agctgaaaga cttggctggg agacaaacat tttctttgcc gcttctctga aactaccatt
     2881 gtttgctaca gcgaccacgt atttcatctg ctgaatattc attgctcaaa ctcctcgaaa
     2941 taataaattt ctcttccatt caagggctgg attggtcggt tgttcggtcg agcatactga
     3001 ccatcgaagg ggagaagaaa ctcttggttc agttttccta catgatccat tacaaaccat
     3061 tggattggcc cgctggtagg cggagttgtc aatgagccaa tataatggaa atggcttttt
     3121 tgctccggaa agaacacatc cggatttacc caatgctcat gtttttctaa gttccaactt
     3181 tccccatttt cttgattata cagccaacct tcctgtacca ttagctggta catgacaccc
     3241 acaactagat tctctttttt gctgttcata tgaacaaaat gaaattcaat atcttcctgt
     3301 tttccgttga tgatatgttc agaaggcaag tggaaatgaa tatctgtcaa ataatagcgt
     3361 tctttcttga actctacata actgagttgg tcaaaaggta ctaaatgaat cgtgtttttg
     3421 aattccttgt cagtaaaccg ttgcttttga taatgaaagg aaagcgtgtc tccttctatt
     3481 ttggtttctt cccccttggt caaatgaata ggcgactgca atggaaattc cgcacctctc
     3541 gcaaaccaat cacataaact cgcccaattt tccggacctt tgtctccaga atagccccat
     3601 tcgacatcca tattcttttt caactatact tccccctgca tctcatatca tttttatttt
     3661 tcttctttct ttacaatcgt tgagacggtt tctaaaaacg cctcttttcc ataaaattcc
     3721 tggttcaata gttccggaaa tatctcttta aagaaatact gaacagaagg ccgatgttca
     3781 aagcttttaa tcgtagtcac tgcctcaatg aacatctcct tataaacttt ttccgggtta
     3841 tttttgatga tttcatcaaa agattcataa agaaggtcaa ccttatctga aatggctaat
     3901 agttctcctt ctacggtttc atcttttcct tcgaacaacc gacggcgata aacttcttgg
     3961 aactcagctg gaatctcttg gttgatgaat tcgtccgtca tcttttcttc aactgtttgc
     4021 agcatccctc ttaactcttt gcttgcgtat ttcaccggcg ttttgatatc accaataaaa
     4081 cgctctgtgt agtcatgatt caatgatttt tcatagagca gtttccaatc gatcggttgt
     4141 ccattcgctt cttcaatgtc tcctagaact tgcgcaatag atgctacacg atacgaatgt
     4201 gctgcaactg tatgctctgt atatttaaaa aatccaggtg cccttgtgat cgtctctaaa
     4261 ttgttcaatc ctaaaataaa ttcattcaac cccataaaag ttacctccct ttgagatttc
     4321 tttttccgaa tatcatatca ttaaataact gatattcgca acatttccgc tctttaccat
     4381 ttttgaaaca gctcccttac caaaaaatag agaaaatcta tatataatag aaaaaagaga
     4441 agagattatt ctcttctttc tgctaaccag tcttagaaaa atgaatttta aaaaaatcaa
     4501 aaactgatca cgatagaagt ttcttttcat acctgatgaa aagatgcttc tcgcaatcag
     4561 tttttggatt tttgctattc caattgatga ttctggagac acaaatcgga tttctctatc
     4621 ttcatcaagc gcgcttaaat ctgccaattt tcaggatctt ttttccattc ttttagcaga
     4681 gttaattctt gctcatcgat ataacgatct tctaaagctg cttcaatcaa tacagaataa
     4741 ttggtcaacg tcataagagg gatttcggct ttttcgaaat ttgcttttcc ttttggcaac
     4801 tcatacgtaa agatagccgc aacaccaagg atatccgcac cttctctttt tgctgcttca
     4861 gctgcttcca agacacttcc gcctgtagaa atcaaatcct cgatcacaac catcttctga
     4921 ccttcaaata tacgtccttc gatttggtta cctttcccat gatctttcgc cttacttcga
     4981 atatagacca ttggcaaatc cagtatttcc gctacccatg cggcatgagg gattcctgct
     5041 gttgcagttc cagcaatcac ttgtacttca gggtaagctt ctttgatttt gcttgctaat
     5101 ccttttgcga tctcttttcg gacttttgga tagctcatcg tgattcggtt atcacagtaa
     5161 attgggcttt tgattccact tgcccatgta aaaggattac ttggatttaa aaatactgct
     5221 tcaatttcta ataagtcttt tgcgatactt tgttcaacat tcattttagg ttagctccat
     5281 tctgtttgaa tcgtttgata tgctttataa ggattagttg actgagtgat tggtcgacct
     5341 ataacgataa aagttgagcc gatctttttt gcctgagaag gtgtgactac tcttgtctga
     5401 tctcctgcga ctgttccttc tggtcttata cccggagtta agcaaacaaa ttcgtctcct
     5461 gccatttctt tgatcgcttc tacttcccaa gcagaagaaa ccacaccatc taatcctgct
     5521 tttttggcac agctagcata atggatcaca cttttttcta aagaaacttc gattagttga
     5581 tcggcatgca tttcttcttc acttgtagaa gtaagctgag tgatagctag aagtagcgga
     5641 cgttttccac cttctggagt cccctcttct aatcctcgca ttgccgcctc catcatacga
     5701 ataccgccag cggcatgaac acaggtgaga tcaacaccaa gctttgccaa tccccgcatt
     5761 gctttttcga ccgtattcgg aatatcgtga agtttcagat ctaaaaatac attatgacca
     5821 gcctctttta gataacgtac gatttcaggt ccttcttggt agaacaattc cattccgatt
     5881 ttcacaaaca atttttcttc tttcgggaaa aatcgaagaa agtcttctac ctcttgccta
     5941 gttgaaaaat ctaatgcaat aattggtcgt tggtctagtt gattcatcgt tcacgttcct
     6001 ctctcacttc tttgattaat ctttccagcg attcgatgcc tagttcgctc attcgcacag
     6061 ggagctcttt aattagttta ggacagatat acggatcggt gaaattcgcg gtccctatag
     6121 ctacagcact tgctccagcc ataaacatct caagaacatc atccactgtc tgcacgccgc
     6181 ccatcccaat gattggaagg ctggatatac ttgctacttg gtgaatcaaa cgaatagcga
     6241 caggcttgat tgctggtccg gagagtccac ctgtttgatt ggctaaaatc ggttttcttg
     6301 tcttaaggtc aatccgcata ccaagaagag tattgatcat cgtaaaccca tctgcccctc
     6361 cagcttcgat tgcctgagcg attggaacga tgtctgttac atttggggac aatttgacat
     6421 agacaggaac agaagaaact ttctttactg cttgtgttaa ttgaaaagca acatcagaat
     6481 ctgttccaaa agcaattccg ccatgtttga catttgggca agaaatgttc agctcgattg
     6541 ctttcacatt tggtgcttcg ccaattttgc tacaaacttc tacatagtct tcttcacaag
     6601 caccagcaac atttgcgatg atgggtaact cattgtactc ctctaattct ggaagaagtt
     6661 tatgcataac aacgtctaga ccaggatttt gcaaaccaat tgcattcaac atgccagatg
     6721 gtgtttccgc cactcttggc gtgggattgc caaagcgggc atttggagtc gttgccttga
     6781 ccatgatcga tccaagctga ttcaaatcat agtattttcc gtattctttg ccaaaaccga
     6841 aacagccgct tgccggcatg ataggatttt ttaattctag gccaggaagt ttgaccgcta
     6901 aaggattctc attcatataa ttacctctcc tattttaaaa atcggtcctt cgtcacaaac
     6961 tttcacactt tttgttcctg tttcatcttc cggcgtatga caaacgcaag catagcaagc
     7021 ccctatccca catgccatgc gggattctaa tgataactgg acattttgat gcgaagaaaa
     7081 cagctgttcc actgtcttca ataacccgtt tgaaccacat gcaaaaacag ctgtaggttc
     7141 cgtcttcgtt cctaacaata aattaccgac atttccgtgc ataccgtatg ttccatcgtc
     7201 agtagcgatt cttgtttctc ctagacgatt aaattctttt tcataataaa cgacttcatg
     7261 tgtggcaaaa cctaaaaaat gcgttggctt aattcctttt tcgactaact gttttgataa
     7321 ctcatataga ggagggacac cgattcctcc accgattata aatacttcgt ctcctgtatg
     7381 aagattggac aaatcaaaac cattgcctaa aggacccatg acatctaaat ggtctccggc
     7441 agaaagagta gagaagactc tagtcccctc accttccacc cgataaatga tacggcaagt
     7501 atttgtttcg tggtcgatat gattcaagct aatcggtcgg cgtaatagca agtctgcacg
     7561 cggcacgaga atatgcaaga actgtcctgg ttgctgcatt tcttttacta gatcaccttt
     7621 caatgtcatt tcaaaaatct ttggtgccag ctgtttttgc tgaacgattt ccatcatttc
     7681 ttgtttcatc tgtcccctcc tgcaacggtt tttcgtcttt ttgtcttaac atgtgctcgc
     7741 cgttaaatag ggttagttga aaaagcacgg gattccatca ctcgaacgat cgcatctgct
     7801 gtgtctaaag aagtaaacaa tggaatacca tgttcaactg cttctcttcg gatgatgaaa
     7861 ccgtctttag aagcatttcc ccgatcttta tcaatcgtat tgacaaccac ttgtgtttta
     7921 ccatctctta ttaagtcgac tacatttttt tctgctgctt cctcagaaat tttcgctact
     7981 gtttcaacga ccagaccatt tttttcgaaa taagcagctg tatgtttcgt tcctatcaga
     8041 ctatagccaa tttctgtaaa acgtttagct aattctagtg cttcttcttt tgtttcgtcc
     8101 gcaatcgtaa ataatacggc tccatattca ggcaagtgta atccagaagc ttcgaatgcc
     8161 ttgtacaaag ctttgtctaa ttgtctgtca gatcccatta cttctccagt cgatttcatt
     8221 tcaggcccta agtaagtatc aactttctgc aatttcgtaa aagaaaagac aggtgcttta
     8281 acatgaactt cttttgtttc tggataaagt ccgttttcat aaccaagtgt ctttagtttc
     8341 tctcccaaaa tagctttcgt cgcaatttgt gccattggaa ttcctgtcac cttacttaag
     8401 aatggaacag tccgacttgc tcgaggattg acttcgatca cataaacctc attttcatgg
     8461 atgacaaact ggatattcat catcccgaca caattcaaac ctaatgctaa gcggatcgtg
     8521 taatcttcga tcgttttctt gatcgattca gataatgttt gaggtggata cacagccatt
     8581 gaatcccctg aatggacacc agcacgctca atatgctcca tgatccctgg gatgagtact
     8641 gtttcaccgt cacaaatcgc atccacttcg cattcaccac caagcaagta acggtccact
     8701 agtactggat gttctggaga tgctttcaca gcatgttcca tgtaatcttc taaatctcgc
     8761 tggttttcaa cgatttccat cgcacgtccg cctaatacat agcttggacg aacaagaact
     8821 ggataaccaa ttctcgaagc gataaccacg gcttcttctt tacttgtagc agtatcccct
     8881 aaaggttgag gaatatccaa ctctttcagc gcttgctcga ataaatcacg attttccgca
     8941 cgatccaagt cttcgatcgt tgtccctaag atttttacac cagcagcagt caaaggttcg
     9001 gctaagttga ttgctgtctg accaccaaat tgaacgatta ctcctatcgg ctgttctagc
     9061 tcgatcacat tcatcacatc ttcgaaggtc aatggctcaa aatataattt atccgaaata
     9121 gaaaagtcag tcgaaactgt ttcaggattg ctgttcatga tgattgcttc gtatcctgcc
     9181 tgctggattg ctttgacaga atgcacagtc gcatagtcaa attcaacccc ttgtccgata
     9241 cggataggac cagatcccag tactagaatt gagggctttt ctgatcgtga actttcattt
     9301 tcatattcat aggaactata aaaataaggt gtttttgatt cgaattccgc tgcacaagtg
     9361 tcaaccatct tatatacagg aatcaaatca ttttcttttc tcatctggcg aacggcttct
     9421 tcgtttattt cccacagctc cgccactttt ttgtctgtaa aaccgttttg tttcgctgtt
     9481 ttcaatatct tgtcatccaa cggctgctgt ttcaactgct gttcgatttc tacgatatgc
     9541 aacagtttat ctaaaaagaa taaatcgatt tttgtcaaat ccgctaattc ttctatggtg
     9601 taccctcttc taatagcttc agctacataa aacaggcgat catcttgtgc tcgaaccact
     9661 ttttggatta ggtcatcgtc cgtgatgtct gccaactcag gaagttcgat atggtgtgta
     9721 ccgatttcta aagaacgcac agctttcaat aaagactctt cgatattccg accgatagcc
     9781 atcacttcac cagttgcttt catctgcgtg cctaaaagcc gttctccgtt ttcaaactta
     9841 tcaaacggcc agcgaggaat tttagctaca acataatcta atgcaggttc aaattctgca
     9901 aaagtcgttc ctgtaacagg atttttcatt tcgtctaatg ttaatccaag cgctatttta
     9961 gcagctagct tagcgatcgg atagccagtc gccttacttg ctaacgcaga tgaacgagaa
    10021 acacgcggat tgacttcaat cacataataa ttaaaactat gtggatcaag agcgagttgg
    10081 acattacagc ctccctcgat cttaagtgct cgaataatcg aaagagaagc atcacgaagc
    10141 atttgatatt catggtcaga caatgtttga ctaggggcaa atacgataga atcacctgta
    10201 tggataccca ccgggtcaaa attttccata ttgcagacaa cgattgcatt atcagccgaa
    10261 tctctcatca cttcatattc aatttcttta aaacctgcaa tacttcgttc aatcaaacat
    10321 tgggtagcgg gggaaagact taatccattt tctgatacga tacgtaattc ttcttcattt
    10381 tcacacatac ctccaccagt tccgcctaaa gtaaaggctg gacgaacgat gacaggatag
    10441 ccgattttat ttgcaaaatc tacagcttct tctactgtat tgacgatttc agactcgggg
    10501 attggctgat tcaattcttt catcaactgt ttgaatagat cacgatcttc tgcttgatcg
    10561 atcgcagaaa gctttgttcc tagaagttct atgttcaatt cttctaaaat acctgatgct
    10621 gccagctcca ttgccatatt gaggcctgtc tgtccgccta aagttggaag gagagcatct
    10681 ggacgctctt ttcgaagaat ccttgataca aattcaagtg tgatcggttc gatatatact
    10741 tgatctgcca cttctttatc cgtcatgatc gtcgcaggat tagaatttac taaaaccact
    10801 tcatatcctt cttcttttaa ggcaaggcag gcttgtgttc ctgcgtagtc aaattctgcg
    10861 gcttgaccaa tgatgatcgg accagatcca atcaccataa ttttcttgat atctgtacgt
    10921 ttcggcatct gtttactgct cctttcctgc gtccatcatt tccataaatt catcaaataa
    10981 atgtagtgca tcatgcggac caggggcagc atctggatga aactgcacac tgaaagcggg
    11041 ataatctctg tgtttgactc cttctactgt tccgtcattt acttctacat gagttacgat
    11101 gagtttttct ggatcaatcg tggcttcatc gactgcatag ccatgatttt gagaagtaaa
    11161 atcaattctt ccggtcgcta cttctcgtac cgggtgattt aatcctcgat gtccgaattt
    11221 cattttatag gtatctgcac cgttggctaa agcaaataac tggtgaccga gacaaatacc
    11281 aaaaatcggt acttttcctt ggattttttt gatcatttcg attgcttctg gcacatcttt
    11341 tggatcacca ggtccattcg tcagcatgac tccgtctggt gatagcgcta aaatcgtctc
    11401 ggcatctgtg ttatagggaa gtactgtgag gttacagttt cttttactca attctcgtaa
    11461 aatactatgt ttcaagccga agtcaatgac aatgactttc cgacctgttc ctggacttgg
    11521 ataaggtttg ttagttgaga cttgctgcac ttgattggtt gctagaacgg ttgctttcag
    11581 ctgatcaaat gcatgttcga aggaatctcc agcatcaacg atactagctt tcatcgttcc
    11641 gacttcacgg attttgcgag taagggcgcg cgtatcgata cctgatattc caggaattcc
    11701 tttttgtttc aaaaatgaat ctagcgtcat ttggtttcgc cagtttgaag ctacacgtgc
    11761 atgttctttt actactactc ctttgcatgt tggagaaatc gattcataat catctctatt
    11821 taccccataa ttacccacca taggataggt aaatgtaatg atttgaccgt taaaactttg
    11881 atcagtgata gtttcttgat aaccagtcat acttgttgta aaaacgattt ctccgaagac
    11941 actagcatct gcaccaaaag cctttccttc gaaaactgtt ccatcttcta atataagcaa
    12001 acgttccata cttttattct cccttctgcc acacaagttt tccgttgaca aaagtcattt
    12061 gtgtttcgcc ttttactttc cagccgataa aaggtgtatt ctcccctttt gataaaaagt
    12121 cttctttatc gatcgtgcaa gtttgtgtaa tatcaaaaac tgctacatca gcgggggcac
    12181 caaccgttag cgttcctgca ttcaaaccaa aaatctcagc cggtttaact gccaaccaat
    12241 tgatcacttg ttccaacgtg aatcgaccag tttctacaaa atgagtatag atcagttgaa
    12301 acgctgtttc gcttcctacg ataccaaatg gtgatttcat gaaactttgg cttttttctt
    12361 ctaatccatg gggggcatgg tctgtagcga tgcaatcgat cgtaccgtca agcaagcctt
    12421 cgatcaaagc ttctctgtcc tcccgtcctc ttagcggcgg gttcattttc caaaagccga
    12481 aatcttcggg aatatcttcg tcgatcaaaa taagatgatg gggtgaaact tctgctgtta
    12541 cgtggattcc tgcttttttt gcgtctctga ttacacggac actttcttta gtagaaacat
    12601 ggcaaacatg gtaatgaaca ccagttgctt cagctagcaa cagatctcta gcaatttgag
    12661 aagactcggt tacgcttaag atcccgggaa gaccaagttc ttctgccttc ttgccagcgt
    12721 gcataacgcc gccaaataat aaagattcat cttctgtatg ggctactagg gctttattgt
    12781 tttttgcagc ttctttcata gccagataca tcgtcccagc tgtttggaca cctacaccgt
    12841 catttgtgaa agcaaaagca ccttcttcaa tcagtgcttc ttgatcaact aatttctcac
    12901 ttcgcagatt ttctgtgatt ggcgcatact gcaatacctt cacaactgca tcttttctaa
    12961 tgatgtcata tacttgtctc agcttttctg ccgtatcagg tacaggattt aaattcggca
    13021 tcgcgcatac agtagtgaaa ccgccacgag cagctgagcg agtcccagct tcgatcgtct
    13081 ccttgtatgt aaatcctggc tctctcaaat gaacatggac gtccactaaa ccaggtgtga
    13141 tcaattgtcc ctttgcatca aacacttcat caaattcggc ttctgagaat cctgtaccga
    13201 ttgctttgat tcgcccatct tctatccaaa tttctgctgg tgtcgtcata ttttttcgtg
    13261 tattgatctg accgttttta atgagtgttt tcattttttc atcctcctat aattacgctt
    13321 ttccatttag cactgcttct aagattgcca ttctgacgaa tactccattg ctcatttgct
    13381 gaacgatccg cgactgtaat ccttccacta atgaatcagc taattctaca tcacggttaa
    13441 ctggtgctgg gtgcataatg attgcatgtt tttgcaactt cttcgcacgc tcaactgtta
    13501 acccatgttc ctgatggtag ccttcttttg agaaactttc ttttccatca tggcgttcgt
    13561 gttgcacccg aagcatcatc atgacatcaa cttgatctag tagttcatcc aatggcatat
    13621 aatgtccgta cacttcgaat tcatcgtcgt accattctct aggacctgag aagaatactt
    13681 gtgcgcctag acgttttaac atctgcatat tggatttagc tacgcgagag tgagtgatat
    13741 cacctacgat ggcaactttc agaccttcaa aatgaccaaa ttcttcataa atggtcatca
    13801 aatcaagcaa gcattgggtt ggatgctgtc cgcttccgtc tcctccattg ataatcgaac
    13861 actggattgt tttactttgt atcagttcgt cataatagtt ctcatcacca tgacggataa
    13921 ccgctacgtc tacgcctaat gctgacatcg tcagtactgt atcgtatagt gtttcccctt
    13981 tttggacaga gcttgtgctt gcttcaaatt caatcacttc tagccctagt tttttctccg
    14041 ctacatcgaa gcttttatgt gtgcgagtac tattttcgaa aaagaggttt gtagcaaagt
    14101 actggcgctt ttgcggagtc caattagcac cttgcttgaa ttcctgccct ctgcggatca
    14161 agcccataac ttcttgatca gtaagtgcct caacggtcaa taaatgcttt aagctgatac
    14221 gttctgattt gatgatcata atgtttcctc cttagttttc ttcgcttcgt gaagtttctg
    14281 gtaagataag atttaaaatg atgccaagta cagttgctaa tgccattgcc gacaatgtaa
    14341 atgtgcctac ttcaaagacg agtccgccaa ttcctactac taagataact gaagcaatca
    14401 acagattttt ctttttatca aaatcgattt tgttctcgat taaaatcttc attccacttg
    14461 cggcaattac tccaaataag ataaaactga ttcctgagat gactggtcca gggatactta
    14521 aaatcaaagc gcttaatttt cctacgaatc ctaaaatgac tgcaaatact gcagctccac
    14581 cgatcacaaa cacgctatgg acacgtgtga tagccagaac accaatgttt tcaccgtaac
    14641 ttgttacagg aggacctccg acaaaggctg ccacgatttg tgctaatcca tcacctgtca
    14701 acgtcttatg aagacccggt tcttcaaaga aattacgttt agttagttta ttcaatacca
    14761 taagatggcc gatatgctct gtcatcgtca caaaagcgat gggtgccatt gttgtgattg
    14821 ctccaagata tagctttggt tcatattgta caaagggaat ctcaaagtta ggaacagcaa
    14881 accaggcagc gtctacgatt ggcttagtat ccacgattcc aaacagcaag gctacgatgt
    14941 atccgctaat aatacctagt aaaatcggaa tcaatccgag aaaacctctt agccacatgt
    15001 tgaagaaaat tgttaatcct aatgtgatca gcgctacagc aatatacttg aaatcatacg
    15061 tgccctgatt atacatagca tttgttgctg catttgccgc aagtcccaac ccgataacca
    15121 tgatgactgg accgacgacg attggaggca agattttatc cagccatgct gaaccgatct
    15181 ttttgatgat cagcgcaaca atcaaataga ctaatccagt tgtgactgct ccttgtgcaa
    15241 tcgctggata accatctgtt ttcatcaaaa gctgcatcgc tgcaatgaaa gcgaagctgc
    15301 ttcctaagta cgcaggtatc tttcctttag tagtaaataa gtacaccaat gtacctagtc
    15361 ctgaactgac tagcgcaata cctggatcga tacctactaa gatggggact aacacggttg
    15421 ctccgaacat cgtgaacaag tgttgtaaac ttaatcctac ccaatgtcct ggggttgggc
    15481 gatcgtggat atctaacacc acgtcatcat tatgaaatcc ttttttctcc gacatgtttc
    15541 tctctcctct aatcttcctc tttcaaaatc atgatgcgat cttgtccatc aagctcttgc
    15601 atctctacta aaatctcttc cgcccgagat gttggaatgt tcttcccaac ataatctgca
    15661 cggattggca attctcgatg cccacgatcg accaggactg ctaatgaaat ttttcttggt
    15721 ctgccaaaat ccattactgc gtccattgct gctcgaatcg ttcgtcctgt atacaaaaca
    15781 tcatcaatca aaataacttc ttttccttct aaggaaactg ggatgtcaga agaatgaagt
    15841 tccggttctt ctggattttc ttttttgtca tctcggtata aagtgatatc cagttctcct
    15901 acaggaatat cgatatcttc tagctgcttc aggcgttcgg caattcttga tgcaatgtag
    15961 attcctcgtg ttttgatacc taccaatacg atatcttgga tactatggtt tctctcgata
    16021 atctcataag taattcgagt caatgctcgc ttcatggtta cctgatctac tacttctttt
    16081 gcttgcattt tcattccccc tacttttttg cataaaaaaa ctcctgccct aagaggacag
    16141 gaggaatgtt tagactgagc gtatactgat acactgatag ctatactatg tcaatgagca
    16201 atctacttat ggcgcaccat acgtatcctt gctcccaccc ttcttagcct cacgggacta
    16261 aacttaaagg aattctatta agttttatgc tttcgttgtt aattactata ccgttatcta
    16321 aattaaaatg caatgttttt tcttaatttt tctaaagttt tttcaaaaac ttctggaaga
    16381 ggtgcttcaa agatcatctt ttcgtttgta gtcgggtgcg taaaacctaa catttgcgca
    16441 tggagaaact gaccattgcc tttcaaagtt ttcttcggtc cgtatactgg atctccagct
    16501 aacggataac cgatatactt catatgcaca cggatctggt gtgttcttcc ggtttctagt
    16561 tttaattcaa ccagagtaaa tccttcgaaa cgttccaata cagtaaaatg tgtgacagcc
    16621 gacttcccat cttctcttat cgcctgcatt ttccggtttt ccttcgaacg gccgattggc
    16681 gcattgatgg ttcctttttc gtgaggaatc tctccatgga ctaaagcaac atatttgcgc
    16741 aaagaggtct tatcttttaa ttgctgagct aatgattcgt gtgcctggtc atttttggca
    16801 accattagca gtccggaagt atctttgtcg attcggtgaa caatacctgg acgaataacg
    16861 ccgttgatcg tagaaagatc cttgatatgg tacatcagcg cattgaccat tgtgccatgc
    16921 gtatgccctg ctgaaggatg aacgaccatt ccttgaggtt tgtttatcac cgctacatct
    16981 tcatcttcgt aaacgatcgt caacgggata ttttctggga ccacatctaa tgtttctggt
    17041 tcggggatca agacagtaat ctcgtctcct tgttttactt tgtaatttgc tttgactggc
    17101 tgtccattga cagaaaccgc tccatttttc agccacagct gaatttgcga acgactgtaa
    17161 tcggtgaata aattagtcaa tactttatcg atcctaccag attcatggtg aatcgtcgtt
    17221 ttaagttctg tcattttttt ctttgatcct tttcatctaa aataatataa attaacatac
    17281 aaatcactcc acatacaagt gtcacatctg ctacgttaaa aatcggaaag ctcataaaat
    17341 ctgtctggaa catatctact acatagccaa gacgaatacg gtcgataaaa ttgccaatcg
    17401 caccagctag aatcaaactc aatccaatca tgaaccagcg attacctttt tcacgatttt
    17461 taatcatcaa tgtgagaatc acaacgactg caatgagtgt cactatagta aagaaccaca
    17521 tcttcccttc tagcatactc catgcggctc cagtgtttcg aatatgattc agtgacaaaa
    17581 tacctggtat aagatttttc gtctctccaa gcgctagatt atcagtgatc caccatttga
    17641 ccaactgatc cactcctact agcaaagcgc tgaatatcca ataaataatt aacaaaaaat
    17701 caatcctttt ctatttgttt ataaatatca cgaggtcgta cgatcccgag cagattacca
    17761 tcgggattcc catctttagt gataagtacg gcttccaaag tcccttgctc acgaaacatc
    17821 gcttcaacct cgtaaatatg tgttttagct ggaacaaaat gataatttgt gtactttccg
    17881 ttttggatga tcaaatccaa cgctcgttta tttgttaaat caaccggacc tttttggctt
    17941 tcttttgcta gccaaaaccc gatattacgt aaagtaatca atgctacaaa ttttccttta
    18001 ttgtataacg gaaattgtga ataccttttt tctgctacta tctttaacag ttcaagtaac
    18061 ggaagatctt gttcaaaacc tgttacacgt tttgcaaatc gaggcagcac ggtttcaggc
    18121 tgaatcagtt cttgttcgat cagctggatt cgcttgactg tccaatcatt tggctctgca
    18181 atgacaaaat cgactgcgat ccgatcatgg acgatcgcat tccttaactg agcaagctgt
    18241 aaaagatcat cttcgtattt tctgatcatt aattgttttc tttgagctag gcgtctgaca
    18301 agttcggtaa atcccatatt tcgtgcattt cccatctcgt ctctcatcca tttttcgatt
    18361 cgattgaaac tgattaaaaa aagatcagag tttgccataa ttttcctcct tgatcaaata
    18421 tgcccggttt tttatataga aaaagaacgc aatcaacaca ataactgcaa aaaaactgaa
    18481 aaataaattt tgtaggaaaa cggcccccaa cccagttgct agaaataaaa acggatagat
    18541 ttgaagaatc aagtaattca ttgtgatcac ataagcaggc tttgcttctt tcactgtata
    18601 aacggggctt tttagccaat aaaaggtgac ctgcatcgta aagggcactt ccagatcaag
    18661 cgttatcttt tgttcatgac tgagaacact gcgtttttca ttgttgattc ttagctgaat
    18721 cgagctaccc ataccgtaaa aaccggtttt tcttataaat gtcgttttca tcataccctc
    18781 ctattatgct ttagtatagc tcaaaataca ataaagaaaa agaatgatcc tagtgtaaac
    18841 aaaattaatt ccctatcgtc taaaaaaaga agctgtagtc aaaatacagc tccttcgatg
    18901 gtagattata atcaagctta aaacaatccc aacacttctc cgtcatttgt cacgtccata
    18961 ttcaatgcag caggtttttt aggcaaacca ggcatcgtca taacatctcc agtcaaggct
    19021 actaaaaatc ctgcccctaa ttttgggatg atttcgcgga tcgtgatggt gaaatcttcc
    19081 ggtgcaccta gcagtgaagg atcatcagaa aatgaatact gtgtttttgc catacaaatc
    19141 ggcagtttgt cccatccatt cttcgtaaac tcttctattt gtttttgcgc ttttttgctg
    19201 aaaacaacac catttcctcc gtaaattttc ttgacgattg tttctgtctt ttcttgtatc
    19261 gtttcttcta ggcggtataa tggcttgtaa ttagcttctt ttctatcaat caatcgtact
    19321 acagcttttg ctaaatcaac gccgccttct gctccatctg cccatacact cgctctcttg
    19381 gcaagaatcc cctgattttc acacaagtgc tccaacagtg tgagttcatt gtctgtatct
    19441 gttacgaatt cattgattgc gacgatcaca ggtagctgat attgttccat attacgtata
    19501 tggcgcttta aatttgcaaa acctactttc aaagcatcaa gattctcatt tttaagttca
    19561 tcttttttca ttccgccatg cattttcaat gcacggacag tggcgacaat caccacagca
    19621 tccggcgctt ttttcaagtt aggtactttg atatctaaaa atttctctcc gccaagatct
    19681 gcaccaaatc ctgcctctgt gacaacgtaa tctgctaatc tcaatgctgt cttggtagct
    19741 aaaatactat tgcagccatg agcgatattg gcaaacggtc ccccgtggac aaaagctggt
    19801 gttccgtaaa ttgtttgtac aagattcggc ttgattgcat ctttcagcag taatgccaga
    19861 gcgccttctg ctttcaagtc tccagcagtc acaggctgac ggtcaaatgt atagccgaca
    19921 acgatccttg caagacgagc ctttaaatct tcaaggtctg ccgcaagaca caagatagcc
    19981 atgatttcgc tggcaactgt gatatcaaac ccatcttctc ttggaacacc ttggattggc
    20041 ccacccaagc caacaattac tttacgcagt tcacgatcat tgagatcgac tacccgtttc
    20101 caaatcactc ggcgggcatc gatgttcaat tcatttcctt gatgaatatg attatctaaa
    20161 agagcagata atgcattatt agctgttgtg atagcatgca tgtcaccagt aaaatgcaag
    20221 ttgatatctt ccataggcaa tacctgtgca tatcctccgc ctgcagcacc acctttgatt
    20281 cccatcacag gaccaagtga gggttctcgc aatgcaataa cagatttttt attgatttta
    20341 ttgagcgcgt ctcctaaccc tacagtgatc gttgactttc cttcgccagc tggtgttgga
    20401 ttgatggaag taactaagat caaatgcccc tcagcgttat cctttgatcg attgattcca
    20461 gaaaaattga ttttcgcctt atatttccca tacagttcca aatcgtcttc cttaagatct
    20521 aatttactag ccacttcaac aatcggttct agctctgttt cttgtgaaat ttgcagatca
    20581 ttcttcattt gattacccct tttcacgaac tttattcaac aaaatatctt ctttgttaag
    20641 atatgccaat tatacctttt tttattcaga aaaaaaacat aaaattacag gtttttaatt
    20701 tatttgacgt acttataaat actctttata atgaaggcta tgatcagact ttagaaaggt
    20761 cgtgagtgct ttgaccacag gaattgtaaa atggtttgac aacaaaaaag gatacggctt
    20821 tatcagttat gatgacacag aagaaatttt tgtacatttt actgcgattg aagaagaggg
    20881 attcaaaact ttggaagaaa atcaagtggt cgaatttgag atcatcgaag ggaatcgagg
    20941 aacacaagct gcccatgtca agaaaggcaa cgttcagact accgatacta gtatgcatga
    21001 atagataaac aaatagagtg agacgctaaa cgggatgaac cgtacgtctc actcttttct
    21061 tttcctgtaa aaattcgttt ttttaggttc tgatttttaa aattagcttt ttcttttttt
    21121 cgtataagga taaggaattg attctgattt gggtccgttt ttttcaaaat gatcaaaaag
    21181 gactacttga tctttttttc gcatcatgaa aacagatggg gctttctcat cttgcaacat
    21241 catttgcacc ccagtattag ttggcgtcaa agatcgaatc tgtccgacag atatttctcg
    21301 gtctttccaa aaagcagcat aacgtataat cacagagttt gattttaaaa taaaagaacg
    21361 ccgcaaacct agaaatagaa aaacaagaaa aatacctaaa acaaaattgc ttttccaata
    21421 aggacctgtg ttttctaaag aaagaatcag actaaaaaac agaatgatca aggtcaatga
    21481 ccaatagatg atcgtatgtg ccagttcagg ctgccatcga atattttttt tcattctcta
    21541 acctctcacc gattcagtaa tgatataatc ttaccataac attagtttga acaaaaagga
    21601 gaatctcttt ttatgttaaa agtatacatt gatgcctcta caaaaggcaa ccctggtcct
    21661 agtggaggtg gaatcttgat tgtctatcaa ggtaaacaag aacagctatc tatcccgctt
    21721 tctataggga caaaccatca agcagagttc gaagtctttt taaaaacttt agaatttcta
    21781 aagaaaaacg acttacagaa tgaaacgatt ttctgttttt cagacagcaa aacactagtt
    21841 tctacggtcg acaaaaacgc aacgaagaac gagctcttcc ttccctacct tcaaagaatc
    21901 caagaacttc tatccgaatt ttctttattg atcctgcaat ggttgcccga aaacaaaaat
    21961 aaaggagcag ataatcttg
//
LOCUS       DAKBVG010000022        21536 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000022 DAKBVG010000000
VERSION     DAKBVG010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 21536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.022"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            53..919
                     /locus_tag="OUD09_000645"
     CDS             53..919
                     /locus_tag="OUD09_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCT9691940.1"
                     /translation="MAIDGLAPGAYQLVETQAPTGYELDATPIEFEIERSQTAVVELT
                     KANRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPG
                     VYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVL
                     QGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFK
                     IEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLV
                     FISIKFRKKRNI"
     gene            complement(1343..1513)
                     /locus_tag="OUD09_000646"
     CDS             complement(1343..1513)
                     /locus_tag="OUD09_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCT9691941.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1707..1886)
                     /locus_tag="OUD09_000647"
     CDS             complement(1707..1886)
                     /locus_tag="OUD09_000647"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9691942.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            complement(1896..2120)
                     /locus_tag="OUD09_000648"
     CDS             complement(1896..2120)
                     /locus_tag="OUD09_000648"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9691943.1"
                     /translation="MNGSIKKILRSIDKNLMITEVSYETLQKKKTLIVAAVLFPAPCV
                     CRNCGSAVVDGNRKLMVVKNKKKETIVHFE"
     gene            2273..3385
                     /locus_tag="OUD09_000649"
     CDS             2273..3385
                     /locus_tag="OUD09_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCT9691944.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3463..3756)
                     /locus_tag="OUD09_000650"
     CDS             complement(3463..3756)
                     /locus_tag="OUD09_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCT9691945.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3924..4238
                     /locus_tag="OUD09_000651"
     CDS             3924..4238
                     /locus_tag="OUD09_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HCT9691946.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4259..6238
                     /locus_tag="OUD09_000652"
     CDS             4259..6238
                     /locus_tag="OUD09_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCT9691947.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            6251..6577
                     /locus_tag="OUD09_000653"
     CDS             6251..6577
                     /locus_tag="OUD09_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HCT9691948.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6593..7111
                     /locus_tag="OUD09_000654"
     CDS             6593..7111
                     /locus_tag="OUD09_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9691949.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            7111..8463
                     /gene="celB"
                     /locus_tag="OUD09_000655"
     CDS             7111..8463
                     /gene="celB"
                     /locus_tag="OUD09_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HCT9691950.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8547..10052)
                     /locus_tag="OUD09_000656"
     CDS             complement(8547..10052)
                     /locus_tag="OUD09_000656"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HCT9691951.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(10144..10734)
                     /locus_tag="OUD09_000657"
     CDS             complement(10144..10734)
                     /locus_tag="OUD09_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCT9691952.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10913..12559
                     /locus_tag="OUD09_000658"
     CDS             10913..12559
                     /locus_tag="OUD09_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCT9691953.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12608..13327
                     /locus_tag="OUD09_000659"
     CDS             12608..13327
                     /locus_tag="OUD09_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HCT9691954.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13806..14753
                     /locus_tag="OUD09_000660"
     CDS             13806..14753
                     /locus_tag="OUD09_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCT9691955.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14765..15685
                     /locus_tag="OUD09_000661"
     CDS             14765..15685
                     /locus_tag="OUD09_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCT9691956.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15726..17183
                     /locus_tag="OUD09_000662"
     CDS             15726..17183
                     /locus_tag="OUD09_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCT9691957.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17528..18142
                     /locus_tag="OUD09_000663"
     CDS             17528..18142
                     /locus_tag="OUD09_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HCT9691958.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            18135..19859
                     /locus_tag="OUD09_000664"
     CDS             18135..19859
                     /locus_tag="OUD09_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCT9691959.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19856..21307
                     /locus_tag="OUD09_000665"
     CDS             19856..21307
                     /locus_tag="OUD09_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCT9691960.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         6950 a         3659 c         4360 g         6567 t
ORIGIN      
        1 ccgagaagga gaaacgctac aaaccggtct aactacagga gaagatggaa aactggcaat
       61 tgacggttta gcaccaggag catatcagct ggtagaaact caagcgccaa cggggtatga
      121 attagatgcg acaccgatcg agtttgagat cgaaagaagt cagacagcgg tggtagaatt
      181 gacgaaagca aaccgactta caccaggcgg tgtcgtgttg acaaagatcg atgaccaaag
      241 cggggaagtc ctgcaaggag ctgtttttga gttacaagat gccaatggaa ccgttctgca
      301 gtcaggtcta accacaggag cagatggaaa actggcgatt gacggcttag caccaggagt
      361 gtatcagctg gtagaaaccc aagcgccaac ggggtatgaa ttggatgcga caccaatcga
      421 gtttgagatc gaaagaagtc agacagcggt ggtagaattg acgaaagaaa atcgacttac
      481 accaggcggt gtcgtgttga caaagatcga tgaccaaagc ggagaagtcc tgcaaggagc
      541 tgtttttgag ttacaagatg ccaatggaac cgttctgcag tcaggtctaa ccacaggagc
      601 agatggaaaa ctggcgatag atggcttagc accaggagca tatcagctgg tagaaactca
      661 agcgccaacg gggtatgaat tggatgcgac accagtaact ttcaagatcg aaaaagaaca
      721 agaagtggcg atctttttaa caaaagagaa tagaaaagtt gctgatagct cagatataag
      781 aggaaacaat ccaacaagca gtggaaataa gcatcttcca aaaactggag aaaccatcat
      841 tagccagttt atattgtcaa tattgggtgt gctacttgtt ttcatcagta taaaatttag
      901 aaagaaacga aatatatgag ctaaaataaa aaattgaata agcatagttt ttaaaatcaa
      961 ctagtaccca tcaattgaat ggaaactacc gattttagaa ggggtataca aacaaatatt
     1021 agtatcggag tagagaaggt tgaacccaac cagtaaataa gtaaagacaa ttttgtgctg
     1081 cgttgagaag tgaaaaataa acctttgtag taatactagt taataaaaaa tatacaaaaa
     1141 cactgcgaaa atcattcttg agtttcgcag tgttttttgc ctaatgactc aagtttattg
     1201 ggtaatactt tgattttctt tctacaaata atcagcgatc agttgtaatt ttttctctag
     1261 ttttaatggc tctttgtcaa taaggagaaa aacaagtcgg gtcttttgtc tcggaaagct
     1321 tcaaccaact cgtgaataaa ggttacgcct ccttgaaagg cgtggaaaaa ccaagtaatc
     1381 gatcaatcac caatttcgca tttggtagaa catgtttggt gagctgaaag taggtggcgt
     1441 tcatgtctgt aactaagaat tctatttctt taggattagt gcagctaagg aaatagtttg
     1501 ttaatcgagg aattttttgt gtaggtaaat tttctcgatt aatttttctg tctcgccatc
     1561 cgcggaaata aaactcagtt tatcctcaac ggaaacgtga gaacgaaatt cacaaccctc
     1621 aacactcaag acagaattct tttggaagac tttggtaaat agcttctcaa ctctttcaat
     1681 gtgcgaataa cggttgtcaa agatacctaa caacttttcg aaataaaaaa taacgacact
     1741 ttttcagtca gtaaagaagt gattttatgt ctgacatgat ttgaaatcga gtgccttgga
     1801 cggataaaat aactttgagc gttccaatgg cttcgacagt ttttacaggt atagcgctgc
     1861 ttttttaggc gtataaccat tggcatatga ttatattatt caaagtggac aatggtttcc
     1921 ttcttcttgt ttttcaccac cattaatttc ctatttccat ctaccacagc agaaccacaa
     1981 tttctacaga cacatggagc agggaagaga actgcagcaa cgatcaatgt ttttttcttc
     2041 tgtaaggttt cgtaagagac ctctgtaatc atcaaatttt tgtctattga tctcagaatt
     2101 tttttgatag aaccattcat aaagcatatc gttctctcag ttgtttattt tgtggtgatt
     2161 taatcataca agaaaacgat atgttttttt ataaaaacat gaataggaaa aatagttgac
     2221 aagaaaatga caacgccata tgatgttcca tagtatttac aacctgtcat agatgactgt
     2281 aaaaaataaa agtaaaaaga aaggacgaca acaggtggat ttttatcagt ctctacaact
     2341 agatccgttt attttaaaac aaaaaatacg agaagcagct tctaaaaaag aaaagtgcat
     2401 gtatatttgt tcattgtttt tacgatcatt attcatcgta ttgtttgcca tttgttttat
     2461 cattataatt acaacattat ttgaatcgaa gcataaacca tacgctgtgg ttttattctg
     2521 tatgttgatg agtattcgtt ttgttgactt tggttataag atttcccatt cgatcatagg
     2581 tttagcaatc gtcatgttct cactattagt agcaccgtat gtccagctga tcaaatggtc
     2641 agttatgggc atgttgattc attttatttt actaagcagc atattaatgg caaccgctag
     2701 tgatcccaaa atggggaatg cttctttgta cggcttttca tatctcttta ttgtttattc
     2761 gttacctaaa gattcgttga acaagaattt ctttacacaa acaagtagct tattattttt
     2821 gtttttttgt tggttttcag ttctccttta cagaaaacat cgagaaaaga acaaagacaa
     2881 aagcttgttt aaagagattt ttttgaaagg catgtattcg caggaaaaaa tttggatgct
     2941 catctatgca tttggcatta gtcttttgat cgttgcagga gagtatgtcc cttttcagcg
     3001 attgatgtgg gcagggtttg cattttcttc tattgtatct agctatggat tgatgtcgat
     3061 tggtttcaaa gaacgagcag tagaccgaat cattggctca ttgattggat gtgttttatt
     3121 tattggtatc agccaattta ttccttttaa ctgggtcggt atattaggtg gtctagcttt
     3181 aggtgtttgt tctacctata gatataaaac agtttttaat tcttttggtg ctttggcaat
     3241 cactgccagt ttattcgggg ttccaggagc agtaacgatc cgcatttttg agaatatcct
     3301 tggggtatgt ttaggaatca tgtatattgg ggtgactgaa atacttatcc gaaaaataag
     3361 agaaaaacac ggtttgaatc attagatgag atcgaataaa aaataaggaa aaagtgaaga
     3421 tacacatgga tgataaattg ctatcattcc atgtgtatct ttttacggta aatcagtgaa
     3481 acgttcgact ttcattttta atttatactt tttacggagt tcgtttattt gagccatcat
     3541 ttcggatttg tgatgctttt cgattgcttc ttcatctttc caacggtcga tcagtaaaag
     3601 agattctgga tcttctagtg gaaagtaata gtcatatttc tcgtttcctt cttctgcacg
     3661 aatcgctttg acaatgtttc tttcgaccat ttcttctgca aattttctgg cttgtccatt
     3721 tgtacttgta tacaaaatat tcactgtgat actcactaaa tcccctcctg aaaaaattct
     3781 atcactagta tatcacggtg ccagaaatta ttattcaaga tatgatacac attttttccg
     3841 caatgctacg gaaatatata ttttgaatag aaaacgctta caagatatgc ttggtttata
     3901 aaaataaaag gagtgattga tttatgaaaa aagcattgat tatttgtgcg gcaggaatgt
     3961 catcctcgat gatggcctca aagacaacgg atttctttaa gcaaaaaaga gaagaggtac
     4021 tcgtagatgc agtttcagct acagaaggcg ataatatgat taagaatagt gattttgatt
     4081 tatttctgat cagcccgcaa acgacgatgt ttttggataa attcaaaaaa ttaggagaat
     4141 ctgttggaaa gcctgttgtt agtatccctt tccaagcgta tgtcccaatc cctacaggca
     4201 tccagaagtt agcagaggtg atcgaagaga acatatgatg gaataggagt ggatgataat
     4261 gaaacagaag gaaaaattgc tgttgagata tttgattgat cacaagaagg agtatgtcac
     4321 cagtcaacgt ttagcttctg aattgttatt atctgatcgg acaatccgta attacttgca
     4381 gcggatcaaa gagttagtag aaaaaaacgg aggtaagatc attgctaaac caggttatgg
     4441 ctaccaactg catattttac aaagactgac atttgacttg tttttgagtc ggcaagaaat
     4501 agtacctgga tatgctagag aaccgcaagg attttatgag tccgaagatc gacaaaagta
     4561 tatattgaac caattgcttt tggaagatgc cattcttttt atggatgatt tggcagaaga
     4621 gctttatatc agtcgttcca gtttgacaaa ggacatgcaa gaaatcaagg agcgattgat
     4681 tccatattca ctaaaaatcg tttctaagca tggtcaaggg acatggatcg aaggggaaga
     4741 aagaaatcga cggcatttta tcatggatac tttttttgga aaccattatg gaaattcatt
     4801 gaaagaatat ttagggcaaa gccgtttttt cccagacatc agttttgaag agctagtcat
     4861 tatcattctc gatgaaacaa gagaagctaa gttaaaagtt tcggatttta tcatacagaa
     4921 tctgacttta cacttggctt tggggatcaa acggctccga gaagggtttg aaatcaagga
     4981 attaggcatc gaacaagaag tttctaagcg agtagagtat caagtggcgc agcggatcgt
     5041 tcaacgtatt gaggtaatcg caggtgtatc gtttcccgaa gaagaaattg cttatttgac
     5101 attacatctc atggctaagt cgaatcacaa agaaaatcaa aatgatcagg aactatcaaa
     5161 ggaattagcg aaagttctga aaagattgtc tcaggaattt catttttcat tagtagagga
     5221 tctccagctt ttcaatggac tactcgatca tttgtcaccg atgatgattc gattgagccg
     5281 aggtattcgc attgagaacc cattgactga ggagataaag aaagaaaatc ccaaagtctt
     5341 tgacgcagta aaaaggcatt tcagcaacat gccggctttg aaaaactata ccatcaatga
     5401 agatgaatgg gcctatttgg ctttgcatct catggcggca ttggaaaaag aaagagctgc
     5461 gcataaacta catgcattga ttatctgtgc aacgggttac ggcagtgccc aactattaaa
     5521 gaatcgcgta gtcagtgaat tcggtaaaaa tattacagtg gtaagtgtaa aaggctatta
     5581 tgagatcaat gaaacaactt taaaaggaat cgacctgatt atttcttcta ttgatctttc
     5641 gacaatgttt ttcaaaattc cagttttaca tgtcagtatt tttttgaatg aagatgatgt
     5701 tcgtaaaata cgaaaggtag ttggagaatc tataccctat cattcctcag atagaccgat
     5761 accagcattt tctctacagc aaaaaaagca gatttacacc gaacagttgt ctgaaaaatt
     5821 tttcaagact tatacgtacg aaccgaaaaa ggaagaaatt cttcatgatt tgctgcaaaa
     5881 gctgagcata aatgaagaag aaggatatat aagagaaatg cgaaagcaga tccagcaacg
     5941 tgaaaatatg ggacaaatca tattcagtga tacagtggca gtcccacatc ccgctgttcc
     6001 agttggaatc gaaacaaagg tagccgttgc attgattcct tctgggatgc agtgggatca
     6061 atatcaagaa attcattttg tttttttagt ttcgccttct tatgtcgaaa atgaaggaat
     6121 tacggtcgtg actaaagcaa tcgttaggtt agttgaccag ctaaaagtac agcaggaaat
     6181 cttagctgaa cctatttttg agaatttcag cagtaaattt atcaaactga tcgaataagg
     6241 aggaaaacag atggctgaaa aaataaacag tgaaacacta caagtgactt cttttgatat
     6301 cattttccat agcggaaatg cacggacact gatacatgaa gcatttcgct cgatgcgtaa
     6361 agagatgttt gaagaagtag aagagaagtt gcgacaagca aatgaagaga ttcttcatgc
     6421 gcatcagtca caaactcaac ttctgaaaga atatgccagc gggacaaaaa tagaaatgga
     6481 aattatcatg gtccatgcac aagatcatct gatgacgacg atgacattat tggaagttgc
     6541 acaggaaatg aattatttgt atcaaaaaaa taaatagaaa ggaaggatca ttgtgcagga
     6601 tacagtaaaa aggaaaatcg aattagaaaa aaagcaactt agtttgaaaa acatgtattt
     6661 caatcgttat ctaatcgtac gttacctgac tgcctttttc tttttcatga acatgcaatg
     6721 gatgattctg cttctatcag ctaaaagttt aggaagtagt ttaccgatga ttctgatttt
     6781 agctattttg cctgcagtag gagaacaagt aaagctttat cgaaaacacc aaacgaacgt
     6841 tccatggaca aagtggtatt ttttgttcca gagtgtcttt aatttattag tgattccagt
     6901 gttgtttacc tcaggattta cattcttgta tccttttatg gcaaataaca atggaggaca
     6961 gttatttgtt ttcattcttg ttgtaagcgg tatttttgcc agtgcattta tacagtatcg
     7021 tttaaagaaa atttcactca accaagacca acagtatatc cgtattaaac aatatgaaaa
     7081 agcgttatat ttaggaaagg agaataacta atgtttgatt ttttgcagaa atacctgatg
     7141 ggacctatgg ggaaaattgc gcaacttaaa atcgttcggg ctgtcatggc agcggggatg
     7201 gcttctattc ctttcacgat tgtaggctcg atgtttttgg ttttaaatat tttgccatta
     7261 ccgttcccat ttttagaagg attttttaat gcaacttttt tcaaagttag tgacttgtac
     7321 atgatcgtca atacaatgac gatggggatt ttatctattt actttgccat tgtgtttgct
     7381 tatgaattaa cgtcaattga acgagatgaa caaaaattaa atgttaatcc gctcacgggg
     7441 gcattactat ctgtttttgc attctttatg tgtatccctg aattgatcat gactgatggt
     7501 aaaatcagct tgattgcttc tatcaatgag acagaaactg tagtaaatgg tattaggatg
     7561 ggctcttttg tcgaacgttt aggaacttca ggaatcttta cagctattat tatgtcgatt
     7621 attgcggtcg aattgtattg tatgtgtgtc aagagaaatt gggtcataaa aatgccagat
     7681 gttgttccgc caggagtttc tcgttctttc actgctctga tcccaacatt tgtcattgcg
     7741 tttgtggtga tgatcatcaa tggattatta gttgcactag gaacggatat cttcaaaatg
     7801 attgctatcc catttgggtt tgttacgcag ttgacgaata catggcttgg tgtcatggtg
     7861 atttatttct tgatccatgc attgtggatc gtagggatcc atggagcgaa tatcatcact
     7921 tctttcttga ccccgatcgt attagctaat atggcagcta acgcacaagg cgcaaattat
     7981 ccgttggcag gagaattcaa caattcttat gtaaccgttg gtggatcagg tgcgacttta
     8041 ggattgatca tctttattgc attcctcgcg aaatctgatc agctgaaagt gttaggaaaa
     8101 gcctcacttg taccaggatt attcaatatc aatgaaccaa tcattttcgg gatgccaatc
     8161 gtctacaatc cctatttagc ggttccgttt ttcttagctc cgatggcttc tgctacactc
     8221 gcttattttg ccattcggct ggaaatcgtc aaaccgatgc ttgcacagat gccgtggcct
     8281 tcaccagtcg ggatcggagc atttgttggt agtggtggtg attggaaagc tgctgtgtta
     8341 gcagtcattt gtgcaatcat cgcattcatt atctggttcc catttatcaa attctatgat
     8401 aataaattat taaatgaaga aagagaaaag gcagcagaat tagctagtga aaagttaaaa
     8461 tgatttctaa gaaaaaagga actgtgacaa aaaatcacag tccctttttt tcttagaaat
     8521 gggaaaccac tagtatataa ataccattat tcttcaatca accgcttcac gattgcataa
     8581 tccccttcat aaggttcatc tacaccattg attttttgat agggatccac gccttttccc
     8641 gcaataacga ctgaatcttg cggagcgctt tctgctaaag cgcgtcgaat cgcttcagga
     8701 cgatccattt cataatgaac gatcaaatct ggattagtaa ttgcttcatt gatttcttcc
     8761 gcgatttttt tcggatcttc aaatgctggg tcatctgccg tcaaaaaggc gatgtcagct
     8821 gtttctgata gcacttttcc aaaatcatgt cgacgggaga ttgctttgtt tcctgggctg
     8881 cctaatacaa caattacacg gccatctgga tgttcatttt tggcaaattt cagcaatgtt
     8941 ttcaagctta gataattatg tgcatagtcc acatagacat gcgctccatt ggattggatc
     9001 aattgatcca ttcggcctgg tacacgagct tcttttaatc cgtcttgtgc atcttgactg
     9061 gaggcacctg ttagtgctgc ggcaatgatc gcactggctg cattttcttg gttgaatcct
     9121 ccagccagca aaatatcata agcagtatcg cagatagcta agcgatcttc atgagaagat
     9181 aaagtaaaac cgtgagtacc tttatctgaa cggatcactt gataattgct ttcttccgaa
     9241 cgaccataag tgatcgttgg aatctggaat agatcggctg tttccctaag taaatcaaaa
     9301 tagtcacttt catgatttaa aacaactgtt tttgaattca agatcaattg tcgcttgcaa
     9361 tagaaatagt catcaaatgt cggatgttcg atcggactga tatggtcagg cgagatattc
     9421 aagaagattc cgacatctaa agtcaagccg tatacgcgtt gtgttttgta agcttgtgaa
     9481 gaaacttcca tcactagatg agtcatacca ttttcaactg cttctgacat catacgatat
     9541 aaatctaatg actcaggcgt tgtcaaatgg gatttgaaat atgttttgcc atctaacgtt
     9601 gtattcatag tagaaagcaa tgctgttttt ttatttgttg tgtgatccaa gattgctttt
     9661 gtaaagtacg cagcagttgt tttacctttt gttccagtga atccgatcac tttcaattta
     9721 ttttgcggat agtcgtaaaa agccatagca agaagcgcca ttgctttacg gatatccgtt
     9781 acgatgattc ctactgctga attttcgtat ggttgttctg atacataata ttgaataccc
     9841 tgttcgattg ctgaatccaa atattcttct ttgaaattca ttcctttaca gaaaaacaaa
     9901 gtagaagcat cagcttttct tgaatcataa cttaactgaa gaaattcagc tttttctggt
     9961 acatcaagat gccagcctct tgttgaaaca aattctttta atagattttc ttttaatagt
    10021 aaatgacgga tgtcttctaa agagaagctc attgattgat ccctcgcttt attcaatttc
    10081 ttggtttatt atagcacact ctttttaata tatcgtttgt ttccgctatg acaaaatcca
    10141 gctttattca aattttcttg acacagggga caagcggatg agtattttca taaacaatga
    10201 actgactgta atagatagga gaacgaatag tgtctccaac aatccaaaaa cataataatt
    10261 caaaaagacg ataagactat tggtaagtaa aagtccttgc ggcattggaa gccgaaaaaa
    10321 tttgtggaaa ataatcggcg gtatcgttat accgccattt gaggccccga ctgtgtacaa
    10381 tgtgtaaacc ccaagactga acaaaagact tcctgaaatc agtgaaaaga aatgtgagga
    10441 gatcatttcc acttttggaa caatctctaa aacaacagga aaaagcagac ttcccgctac
    10501 agtcttaaaa aatactgatt ttcctaatag gacagcggct agaagcagca tcataagatt
    10561 gatgctccaa acagtaactg agcgttggat agaaaaggcc gtttcaacta aatgtccgat
    10621 ggcgcccact ccggcggaag ctatatcgtg agggagcaag aaccaattca atccaactgt
    10681 gataaagaaa agacctaaaa atataacggg tagttttttt atttcttgtg tcattgatat
    10741 ctcctataaa tgttagccat aaaaggctgt atatttcttt actttaccaa aaaaacgaaa
    10801 ccgaattcaa aaaaacaatt gttcttataa aagttatata aaatcgattg ccagtttctg
    10861 ttttaccaat taaaatgata gtaatgtttt gtagaaagtt ggaatctgac cgatggataa
    10921 tcaaagaaaa ccagcattag atcaattaac accgcaagaa aaaatggcac gaggctctgc
    10981 ttggatgacc gccagtaata tcatttcaag attgctaggc gctgtctata tcattccctg
    11041 gtatgcttgg atgggcgaaa atgctaaggc tgccaatgga ttattcaata tgggatacaa
    11101 tatatatgca ttgtttctac tgatctctac agcaggaata cctgctgcga ttgcaaaaca
    11161 aactgcacgc tataattcat tgaatgaata tggcacttca cgacgattat ttatccgtgc
    11221 attacagatg atgggcgggt taggcctgtt gtttgctttg ttcatgtaca tcgcctctcc
    11281 ttggctagct catgcttctg gtggcggaga agaactgatt ccaacgatgc gttctttgag
    11341 tatcgctgta ttggtttttc cttgtatgag tgtgatacga gggtatttgc aaggcaatca
    11401 agaaatgatg ccttatgcgc tgtcgcagat tgtagaacag gtggcacgta ttttttatat
    11461 gctattggct acattcatca tcatgaaagt catggatggt gactatgtga cggcggtcac
    11521 gcaatcaact ttcgcggcat ttatcggtat gctagcaagt attcttgtcc ttttgtattt
    11581 tttgaagaaa catcaagcgt atacatcggc gtttattcac tacagcgaaa acaaaataaa
    11641 tattgcaacg aaggaattgt tgttagatac cgttaaagaa gcgattccgt ttattattgt
    11701 gggttcaggg gtaacgatct tcaaattagt cgatcagttc acttttatga agatcatgtc
    11761 tgcatccacc gagtattcca atgcacagtt actggatctt ttttcgattt ttagtgccaa
    11821 tccagataag ttgacgatgg tagttattgc attggcaaca tcgatcgcgt ctactgggtt
    11881 accgttgatt acagaagctg ttacgttgaa agatcgagta ggtctggcaa aattgacaag
    11941 cggaaattta cagttgtttt cattttttat gtttccggcg acatttggtg ttatgttact
    12001 tgcctatccg ctaaatacat tattttatac accggattct ctaggaagta atgtactgat
    12061 ccaagcaagt tttgtaggac tcttcctagg actatatatg ctagtttcca acatgctgca
    12121 aggaatgttt gaaaacaagg cggcaattaa ttatttggtt attgggtttc tagtgaaact
    12181 ccttcttcaa tacccagcca ttcgtttatt tgaagtatac ggccctttat tggcgacaat
    12241 gatcggattt gctgtgtctt gtaccttgat cttaaagaga atccatgaag tagcccgctt
    12301 taaaccaagt tttgtctggc gcaggacatt gctgatcttt attcttacct tgattatgct
    12361 tttggcttcg tggatcacaa agatgatttt tggtgctttt ttaaacgaag atagcaaatt
    12421 ccagtctcta ttgttgattc ttgtagtagc aggtataggg gctttagtgt atggttattt
    12481 agctttgaaa atccgcttgg cagaaagact actaggacca ggaatagcgc gtttacgaca
    12541 taaattaaaa ataaaataag tgaatcagaa gagctagatt gatgctcttt tgaaatgggg
    12601 gaaacgcatg cgtttagata agtttttagc agatatggga cttggcagcc gcaaagaagt
    12661 caaaggatta atcaaaaaag gaacgatcga agtcaatgga caagtggtga aaagcgataa
    12721 agcgcaagta aatgaattcg aagatgcggt tacttattta ggagaaatca ttacgtatca
    12781 aaaagatttt tactacattt tgcataaacc tgcaggagtc atttctgcta ctcaggataa
    12841 tcatgatcaa accgtaatcg atttattgga agaccaggat tatcgggaag atctttttcc
    12901 agtcggccga ttagataaag acacagaggg cctcttgatt ttaacgaatg acggggtgtt
    12961 tgcccatcag ttactatcac ctaaaaaaca tgtggagaaa gaatatctag ctgaggttag
    13021 cggtataatg acagaagaag atatccgttt atttgcagaa ggactgatta ttgatggcga
    13081 tgagcagaca ttgccggcaa aactccaaat tgatcaagta gatcaagaga cagaaacttc
    13141 aattatccgt ctgattttgc atgaaggaaa gtttcaccaa gttaagcgaa tggtcaaagc
    13201 agtaggaaaa gaagtcttat atttaaaaag aatccgtatg ggaaaatttt ggctgcctaa
    13261 agatttgaat aaagggcaat atcgacccat gaccaaagaa gagctagaac aagtgagaga
    13321 aaaataattg aatggatagc taaaagaata agcagagtag ttgtagcgcc atctgaaaag
    13381 cagacttgtc cgtctgactt tcggatggct tttttgtgaa atttcggctt atttccgtag
    13441 aagctacttc tggaacaccg tttgttcgga ataagggact gtgacaagac ttttgttaca
    13501 gtccccattg ttccttgtga ctcagagtta gatagtaagg acacgatctc cctaatactc
    13561 aggccaaaac gctgaattca caacctctga taaacggtgt tcaatcagct gaccctcggt
    13621 attagctcaa aaaacggcaa aatatgagga actattttcc cgtttttttc tctaatactc
    13681 aggccaaaac gctgatttca caacctctcc tatactttgt ttcattttcc ctatactttg
    13741 tttatttatt ctcattattg aaagcgctat aattacgttg cgaatgaatc gaaaggggtt
    13801 tttagatgaa aaaacgaaag gggttcaatc atttttttat gaatctatgg cgttataaag
    13861 cacttgtcct gatggctgta ccgggtatgc tttggatgat ttttttcttt tacatacctg
    13921 taatggcgaa tgtcgtagca ttcaaaaatt ttcatatttc agcagatggg ttcttggcta
    13981 gcttgaggga aagtccttgg gtgggttggg aaaatttcaa atttttattt tcttcggatc
    14041 aagcatttct catcactaaa aatacaattt tatataatgt agcatttatc cttttgaatc
    14101 tgctgatctc agtcgtattt gcaatcatta tgagcgagtt gagaaataaa cgactcgtta
    14161 aagtttatca aacgatgtcc ttactacctt attttctatc ttgggtcatt atcagttact
    14221 ttgtttacgc ttttttaagt cctgacaaag gaatattcaa tcagtggatc actggacatg
    14281 gaggagaagc tgtcaattgg tataatgaac cgaaatattg gccgtttatc ttggtgttca
    14341 ttggtacatg gaaagggatt ggttataaca gcattattta ctttgcatct gttatgggaa
    14401 tcgatccgac ttattatgaa gctgctatgg ttgatggagc tagcaaatgg cagcagatca
    14461 aacatgtgac gataccacaa cttattccac taatgacgat tttaacgatt ttagccgtag
    14521 gaaacatttt ccgagctgat tttggcctgt tttataacat tcctaggaat tccggtgcat
    14581 tatatgatgt gactcaagtt ttggatacgt atatctacaa tggcctgaca tccacaggag
    14641 attttggtat gacggctgct gcaggacttt accaatcagt tgtcggcttt gtcttgttga
    14701 tgatcacaaa tacgatcgcc cgacgttttg atagcgattc tgcattattc taaggaggaa
    14761 aaaaatgaaa aaaaagaaag tggagagagt ccaagtccgc tctttcaata agacggctaa
    14821 ttttttctct aatataatga ttgcactctt tgctatttcc tgtattttgc catttatatt
    14881 tgttatcgcc atctctttta ccgatgagtc catattgtcg actcacgggt acagtttttg
    14941 gccagaaaag ttcagtatat ttggttatac gtacttgttc gaccagatgc aggataaaat
    15001 tttccaagcg cttttcgtca ctgtacttgt tactgtttta ggtacactga tcaatagcac
    15061 ggctacttct ttatatgctt atgcgatttc acggtcaaac tttccattcc gtcgtttttt
    15121 taccgttttt tgtttgatta cgatgttgtt ctcaccaggg atggtggcca attatcttgt
    15181 tatgacaaat cttcttcagt taaaagatac gatttgggca ttgatcttgc caatggctgt
    15241 cagtcccttc aatattattg tgatgcgcac attctttaaa cgttcggtct ctgattcgat
    15301 tattgaatcg gcacggatcg atggagctag cgaactgcga atatttgttc agatcgtttt
    15361 accgttagct gttccaggta ttgcgacaat cagtttattt gccgcattgg gttactggaa
    15421 tgactggttc aatgctttac tatacatcca agatgataag ctagttccac tacagtatct
    15481 tttgatgaaa atccaaagta atattcaata tctgacacaa aattcaggag caggaagcca
    15541 attgacaggc ggattagcct cagttccagg agaatctgcc agaatggcga tcgtagtcat
    15601 ctcgacgttg cctattgcga tcagctatcc atttttccag aagcattttg tcaaaggtct
    15661 aacaatcggc ggagtaaaag aataaagaaa caaaaatata aatcataaaa ttaggaggaa
    15721 gaaaaatgaa gttatggaag aaactagcct ttacaggtgt ttcagcgttg ttgttgggaa
    15781 cattagcagc atgcggaggt tctggttcta aagaccaggc agaagctagc aattcggaca
    15841 ctttgcagat gtatcaaatc ggagataaac cagataattt cgatcaattg atggaagtag
    15901 caaacaaacg aatcgaaaaa gagatcggtg taaaagtcaa tatcaattac attggctggg
    15961 gcgattacga aaagaaaatg aacgtcatca tttcttccgg ggaaaattat gatatcgcct
    16021 ttgcgaacaa ctatgtttcc aatgcccaaa aaggagcctt tgctgatttg actgaattag
    16081 cgcctaagta cgctgaaaaa gcttataatg atttagatga agcatatatt aaaggaaatc
    16141 tggtcaatgg aaaattatat gctttcccag tcaatggaaa tgtctttgct caacaagtac
    16201 ttacttttaa caaagcttta ctagataaat acgatctttc aattgatgga atccaatctt
    16261 acgctgatgc agaaaaagtc ttgaaggaat tccatgaaaa agaaccaaac acggcagctt
    16321 ttgccattgg acaaggcttc aaagtccaag ggaactttga ttatccttta gggaatacgt
    16381 taccatttgc tattgatctg gatggagatg attcgaaaat catcaaccaa tacgataatg
    16441 aaacgtatat cggactgtta cgtacaatgc atagctggta caaacaagga ctcattccaa
    16501 gtgacgctgc aacgagcaat aaggattatc cactagaagg aaatacttgg ctaatgcgtg
    16561 gagaaacaca aggaccgtat gattatggcg atacaatttt gacaaatgca gcaaaacaag
    16621 aacttgtctc aaaagcaatt actgttccac taaaatctac agctcaagca caaatggcaa
    16681 attttgtcgt atcaaatact tcaaaaaata aagaaaaatc agtagaactt ctagggctgc
    16741 ttaacagtga tccagaatta ttgaatgggc ttgtttgggg gatcgaggga gaagcatggg
    16801 aaaaagtcga tggttcagat gataaaatca aattgctaga tggttatcag ccaaatacac
    16861 atatgtctgc ttggaataca ggaaacaaca agattctata tacacaagag tctattacgg
    16921 atgacatgat tgccaaacgt gatcaatcga ttgctgatgc agaaacttca ccgattttag
    16981 gattcagctt caacactgac agtgtcaaaa cagaattaag taatatttct aatgtcatga
    17041 atcaatattt agatggattg aatacaggga ctgttgatcc agatgaaaca ttaccgaaat
    17101 tgaaagacgc tttggacaag gctggctatg ataaggtatt gaaagaaatg caaaaacaat
    17161 atgatgagtt ccgacaagaa taataatgaa taaagacaaa ggggttgtga caaaagacgt
    17221 gtcacaacct cttgttccga ataaacggtg ttcaaaagcc agtttctcgg tattaagcta
    17281 aatatatgta aaaatgtgga aagccatatt cacaaaattt ttcttactac tcgaaactaa
    17341 gcggctattt cacaaactct tggtaaacgg tgttcaatca gctaaccctc ggtattcgct
    17401 cacaaaacga caaaatatga ggaactattt ttccgtttgt ttctctaata atcaggtcaa
    17461 ggtgctgatt gcccaacctt tttttgtata atgagaaagt ataaacttaa tgtttggagg
    17521 agaagatatg ttagggaagg ttttggaaga gttatttatc cggatctggg tcattataaa
    17581 actaaccttg tatttctgga tatatacatt tgccggcggg atcatctttg gattaggtgc
    17641 ggcatggaaa acagtcaatg aactatttta tttgtacgga ttcgagtata aagaaatcac
    17701 aataaaaaga ggctggaata tatataaaag aaatttttta agaggcaatc tgctatttag
    17761 tttattttta tctggtactg ctttattgag ttacaattta tatttatctg ttcaaatcaa
    17821 agggctcatt tttttagcta ttgattttat tttacttttt gccttgctct gcctatttgc
    17881 tacttatcaa tacagtatga ttttagatag tgaatatacg atctctgttc ctaatctgct
    17941 aaagctaagt tttatttcgg ttttttccag cttttcgtct tttctaaaaa taattattgg
    18001 tagcggtata attttaggcg tgacttggca attcaaagga ttgatcctgt ttggtgtcat
    18061 tggtcttttg acggtttgga atggtacgat gacaacacat tggcgtgaag aattagataa
    18121 acagttggaa agttatgaat aagtttcgga ttctattttc aaaaggaacc ttactaaatc
    18181 gtctgttgcg gaaatacgcg gttatcatga tcggtgtaac gatgactgct accgttattt
    18241 ttagtatcca tacttgggaa caaaatcaaa aacaggcaga aaacatgaca agtgatgccg
    18301 ttcaaagcac aagcagaatg ctgaatgata aaacaacctt gagtaggatc atcaaaaacc
    18361 agcttgtagg gaattccgaa aagatcgaaa atgtgacgac ctacttaaca aaacccatag
    18421 atcagtatct gatgtatgta tacgaacagc aaaacagtac cgatgaatta gtttctttcc
    18481 caaatcaaat caaagatctc tacgcaaatt atgaagaact aagtgcgata tatatcgtgt
    18541 tgaatcaact accggagtat tatgaatctt ctagagaaaa caaaggtggg gaaataaaaa
    18601 aggggacacc aaaattacta gacgcctttt atatcaagtt gcctatcaca caaggaggag
    18661 ctgagtcagg aagtattttt attggttttc aaaagaaaga actagatatg attttagaaa
    18721 atctaacgtc attcaatgga ctctctttat acatgatttc tggaacgaca aatcgactat
    18781 acacattcca tgataataaa atagctaaag aaacgtttga aagacaagaa atgatcatca
    18841 cagagagtct gaagaaaaat tcacaattgc cgattgacga tctggaaaaa aacaattttg
    18901 ttcaacacca agaactgtct gatgattatc gtatattagc tgtactagat aaaaaaagtg
    18961 taaaaatcga aacgggaaaa aatcttgccc cgctgatcat tggtatcatt attttagaca
    19021 atatactgct tatctttctt tataaaacat tcaaacgata ttcacagcaa gtatctatcg
    19081 tcatgcaggc catggatcaa acagcagcag ggaatttaga gacgcggatc gacataacaa
    19141 acacggaata tgaactgaag gaactttcaa tcggtatcaa cgaaatgttg gatagcatca
    19201 atcagtttgt agaagacatc tacaaattag aaatcaaaca gcaagacgca catatgcggg
    19261 cgcttcaagc acagatcaat ccacactttt tatataatac attagaatat atccgaatgt
    19321 atgcaatcag cgaaggaagt gaagaattag cagatgttgt gtatgcgttt tccgctttgc
    19381 ttcgaaacaa tacgaatcaa gagaaaacga tcactttaaa agaagaactg gacttttgcg
    19441 aaaaatatgt ctatttgtat caaatgcgtt atcctaatcg agtagcctat cattttatga
    19501 tcgatcctga tttggaaaag atcgaagtac ctaaatttgt gatccagcca ttagttgaaa
    19561 attattttaa acacggtatt gattttactc gattcgataa tgcgctaagt gtcaaagtct
    19621 tgcaggaagg caaacgggta aggatcatca tcaaagataa tggaaaaggg atgactgaaa
    19681 aacggctaaa acagattgaa gaaaaactgt ctcatcctaa agtggaactt cacggttcta
    19741 tcggtctgca aaacgtgaat gaacgtttac gtgccagctt cggcagcagt tattatatgt
    19801 cgcttgagaa taatgagaca ggtggactga ccgtttctat tacattcaag gagggatgag
    19861 cgtgtatcaa gtgttattgg tagatgatga atacatgatt ttgaatgggt taaaaaagat
    19921 aattgattgg caaagtttag gctttcaaat agtagcaaca gcagagaatg ccaaagaagg
    19981 actagctgta cttgaacagc gacagattga tttagtagta acggatgtga caatgccaga
    20041 aataaatgga ctggaattta ttgaagcggc tcaaaaagaa cgtcataatt ttgaatttat
    20101 gattttatca ggataccaag aattcgatta tttgaaagga ggtatgcaat taggcgccgt
    20161 gaattatttg atgaaacccg tgaataagtt cgaattggtt gaaagcctca agaaaatcaa
    20221 aacacggtta gatcagcaaa atgaacaaaa aaatcaacaa gaaatctatc aagaactgtt
    20281 attcagtcaa tggttgaatg aagaattgga tgaaataagt gaagaagaaa taataggaca
    20341 aataagtgag aagcagcata ggatagtatt gattcaactg gcaagaactc atgatactct
    20401 tatcaataga tggttgcaag aacggcaaca aatcttttac tatcaacgaa attacggaga
    20461 ccgtgtattg atcacgttgt tgttggaggc agatgaagtt gatggcttct gttgttttgt
    20521 tcaagaaaac tttcctgatc aggaatggct gatcagtatc ggggaagaga cagattcttt
    20581 ggacaaaatc cctgaaacat tccaacaagc gaaagacaat ctacagttgc atcaatttta
    20641 tggagataaa gacgagcatg ttttttacgc agtacaagca gaaacaaaag atcaaaccat
    20701 cgatttttca agtttcagac gcgtgctgca aaataaacaa ttggaagtcg ctcaaaaaat
    20761 gatcactgat ttttttgaac agtttcagtt agcagccatg atgccggaag atatacgata
    20821 ttccagtttt ttactgttta tggagattca acgggaattg attgatctgg aagatgagga
    20881 atatttacaa ggaatcgaaa aaatccatca ggcaaaaacg gtgcaagaac ttcagcagct
    20941 acttctgtca ttcatcaaaa agcatcagag acagaaaaag tacagtgaca atgtggaaaa
    21001 agtaatcgta atcctgcacc agcattatca agaaccactt acactcaaag aagtgtcaga
    21061 aaacctgcat ttaaatgtca tgtatctagg acagctgttc aaaaaagaaa cgaaaaaaag
    21121 tttttcagct tatttgaacc atttacgtat ggaaaaagcg aaacaactgc ttttgcacag
    21181 taatcaaaac atcaatgaga tagcaagtga gattggctac aacaacacca cgtatttctc
    21241 taaattattc aagaaaatcg tcggcagatc ccctaaagag taccgagaaa acagtggaga
    21301 atcataaaag aaaaagccaa tgtaatgaaa taacttataa aaaattgtct agaaatctaa
    21361 ttttgggctg ttcttattac ttgaggcaag gcagcttttt catgacctct cgataaacgg
    21421 agttcaaaag ccagtttctc ggtattaagc caaatttatg caaaaatgtg gaaagccatt
    21481 ttcacaaaat ttttcttaat actcgaaact aaacagcttt ttcacgacct ctccac
//
LOCUS       DAKBVG010000023        21038 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000023 DAKBVG010000000
VERSION     DAKBVG010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 21038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21038
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.023"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            226..432
                     /locus_tag="OUD09_000666"
     CDS             226..432
                     /locus_tag="OUD09_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9691961.1"
                     /translation="MLGQCFGNHARFDSLPATLNAYDGYDYRKKIVKKLYGATYGNVV
                     SVLSVHHQASVTVAEVEDAAVNGE"
     gene            628..795
                     /locus_tag="OUD09_000667"
     CDS             628..795
                     /locus_tag="OUD09_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCT9691962.1"
                     /translation="MYRPQYLEQKYEVITVHKGNGEKVYEYRRPIKSDTYKRKENNEV
                     IPLYGKRIAKH"
     gene            821..1165
                     /locus_tag="OUD09_000668"
     CDS             821..1165
                     /locus_tag="OUD09_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCT9691963.1"
                     /translation="MTEEFYRWLLQLTREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            1170..1451
                     /locus_tag="OUD09_000669"
     CDS             1170..1451
                     /locus_tag="OUD09_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9691964.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            1554..1868
                     /locus_tag="OUD09_000670"
     CDS             1554..1868
                     /locus_tag="OUD09_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HCT9691965.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            1846..3540
                     /locus_tag="OUD09_000671"
     CDS             1846..3540
                     /locus_tag="OUD09_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCT9691966.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            3560..4738
                     /locus_tag="OUD09_000672"
     CDS             3560..4738
                     /locus_tag="OUD09_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCT9691967.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            4701..5387
                     /locus_tag="OUD09_000673"
     CDS             4701..5387
                     /locus_tag="OUD09_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HCT9691968.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            5387..6547
                     /locus_tag="OUD09_000674"
     CDS             5387..6547
                     /locus_tag="OUD09_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCT9691969.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            6557..7432
                     /locus_tag="OUD09_000675"
     CDS             6557..7432
                     /locus_tag="OUD09_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9691970.1"
                     /translation="MKYILCQPAITRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            7429..7740
                     /locus_tag="OUD09_000676"
     CDS             7429..7740
                     /locus_tag="OUD09_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9691971.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            7730..8083
                     /locus_tag="OUD09_000677"
     CDS             7730..8083
                     /locus_tag="OUD09_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCT9691972.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            8073..8474
                     /locus_tag="OUD09_000678"
     CDS             8073..8474
                     /locus_tag="OUD09_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9691973.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            8467..8871
                     /locus_tag="OUD09_000679"
     CDS             8467..8871
                     /locus_tag="OUD09_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9691974.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            8883..9491
                     /locus_tag="OUD09_000680"
     CDS             8883..9491
                     /locus_tag="OUD09_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCT9691975.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            9511..9873
                     /locus_tag="OUD09_000681"
     CDS             9511..9873
                     /locus_tag="OUD09_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9691976.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            10075..13506
                     /locus_tag="OUD09_000682"
     CDS             10075..13506
                     /locus_tag="OUD09_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCT9691977.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASEAEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLSKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDFIVAIGGLTAAFGILLPAILAVSYLFGPMML
                     IIGGIIAVIAGVIVAIKNWGAITDWFSGLWKKFTDWLGDTWESIKDGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQTFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISGIFDNLNISIPHIPLPEFIMEGSF
                     NPLKGQIPHLRVKWHAKGGIFTKPTLLGGMNGVGEAGPEAVLPLKRSVLQEIGDRILS
                     STSVSSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKIQNDNASAWR"
     gene            13557..14294
                     /locus_tag="OUD09_000683"
     CDS             13557..14294
                     /locus_tag="OUD09_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCT9691978.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            14304..16595
                     /locus_tag="OUD09_000684"
     CDS             14304..16595
                     /locus_tag="OUD09_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCT9691979.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVDGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDNGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            16619..18745
                     /locus_tag="OUD09_000685"
     CDS             16619..18745
                     /locus_tag="OUD09_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9691980.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGDYDYSENPNLMPYITEPWVG
                     PLLGNGHTVKDSVKRVITHTKTRTANSGDILSLGLGIPCTAEANNRYLITTLRPSTTY
                     TLSVTMSVGSDWTGETNTIGVRLRYLNEQGGIELPINALIPANVERDKMVTHTFTGIT
                     KDNVTSITNCYVEIFSLNSEYKGTVSVSYDVKLKAHYPNLLDGPYWLGKVPLGENIAD
                     PTVVFPHKTSEYMVYGRRNTENYIADQTYTISMKATKLTVQSFAVYIAAGRVKVGDMK
                     PTEGLANTWELTFTVTKQHIDSGVTNYLEIYQYPSATKGAVQIEWLKLEKGNTRTPNI
                     SEYKYRGTGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINS
                     QLTASINEVDTTAKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKL
                     YREGKTVSIYFVALNGKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSI
                     GADGAIKWRRNSSYGSDYTFAITYTI"
     gene            18762..18911
                     /locus_tag="OUD09_000686"
     CDS             18762..18911
                     /locus_tag="OUD09_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9691981.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            18908..19354
                     /locus_tag="OUD09_000687"
     CDS             18908..19354
                     /locus_tag="OUD09_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9691982.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            19356..19493
                     /locus_tag="OUD09_000688"
     CDS             19356..19493
                     /locus_tag="OUD09_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HCT9691983.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            19531..19824
                     /locus_tag="OUD09_000689"
     CDS             19531..19824
                     /locus_tag="OUD09_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9691984.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            19821..20045
                     /locus_tag="OUD09_000690"
     CDS             19821..20045
                     /locus_tag="OUD09_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HCT9691985.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            20042..>21038
                     /locus_tag="OUD09_000691"
     CDS             20042..>21038
                     /locus_tag="OUD09_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCT9691986.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGA
                     ANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADG
                     IYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDK"
BASE COUNT         7408 a         3182 c         4439 g         6009 t
ORIGIN      
        1 aaattatcaa agcaatgtta ttttgttgct gtctattgtt ttttaattat tcacacgata
       61 actaaaggtg ggtgaagaga aatgattcca ttaataattt caatttttgc gctctgtctt
      121 aatgtctata tgattggatt taaaaatggg caaaataaaa aatagtagca gccaagaata
      181 attttatagt gtcactgtgg cggaaagggt agacgctaag catgtgtgct aggtcaatgc
      241 ttcggcaacc atgcaaggtt cgattccttg ccagcgacat taaatgccta tgacggttac
      301 gactaccgaa aaaagatcgt taagaagcta tacggtgcta cgtacggcaa tgtagtaagt
      361 gtgctatctg tacaccacca agcttcggtc actgtggcgg aagtagaaga cgcagcggta
      421 aatggcgagt agcctcgtga gagcctggta agttctcgtg agtggtgcaa tcccactcca
      481 gcgactttaa gcaaccgagg catcggcggt ttaaaaatat aggggtgcgc aatttcgtac
      541 gcgttttgtg catcgtgcaa gttactatta catattagat cactctttga gtggtctttt
      601 tatttttgca caaaggagga aacaacaatg tatagaccac aatacttaga acagaagtat
      661 gaagtaatca ctgttcataa aggtaatggc gaaaaagtat atgagtatag aagaccaata
      721 aagagcgata catataaacg aaaggaaaac aatgaagtta ttccattgta tggcaaaaga
      781 atagctaagc attaaataag attgcgaaag gagacggaac atgaccgagg aattctatag
      841 atggctatta cagttgacaa gagaagatcg tttggttaag ttctatcagt ctcctaaatg
      901 gcgcaggctt agagagaaag cgatgaaacg agatcactat gaatgccaag agtgtagaag
      961 actaggtaag tatcatagag tagagaacgt tcatcatata aaggaagtca aggatagacc
     1021 tgacttagct ttagatttag ataatcttat ttgtttatgt gttgaacatc ataatgaagt
     1081 tcatggcaga tatcttacag cattagataa acaagagaag aagatagaaa gctttgctaa
     1141 cttcgatgca agtgaaaggt ggtaagtgca tgatcatcaa tgataatggc agagagtatg
     1201 atacagaaaa gattgaagag tattcatctt atactcaggg attaattaaa cgtttgatat
     1261 acgttcgcta tgtaggtatt agggatctgt tatcagataa ctgttgtagt aaatacaaag
     1321 tgaatcaagt aagagaagcg ttgaataaag ataataacgt cgaaagaata aaaaatgttt
     1381 ttggatatag tattgaagag attaattatt acattgactt cgctgaagct ttcattccga
     1441 tggtgagata accccccctt aaaataaatc gcaaattttt tgggggtgat gaaacggagg
     1501 gggctgtcag gaaaagagat tttttcgaac tttatcatga aaggagggct aaaatgttta
     1561 aaaacgaatt gtctcaaaat cgctacagag aaaaattacg ccgctcttta ataagccaat
     1621 tggaaagtca gaaaacaaat attgagccat tcttagataa tgttgatcgt tatatcagtt
     1681 tatgggaaac ggcgatatca ctggaagaag atatatccga gaacggcatt agactggaga
     1741 atggtaaaaa gaatgaatca gtagcgttgc ttgtttctgt caacaaacaa atgggattga
     1801 tgttggataa acttgccatt actcctgaat tggtaggtga agcaaatgaa tcaattcctg
     1861 agttataagc atattgaaaa ttggttcaaa gctatagaag aaggcactat caaggtatgc
     1921 aaagagcaat tattgctaaa aaattatcta gaagaaagag tctttactag agaagatatt
     1981 tacttcgata agcagatggt agaggattca atcaatatac cagcacaata ctttccattc
     2041 gaattaattc cgtgggaaaa atttctacaa tgttttattt atggtgttcg atggaaaaaa
     2101 gataaaacac tagtgttcaa tagatatctt tcattaatgg gacgtggtaa tggtaaaact
     2161 ggttttgctt cttggaacaa cttctttcta ctaaccgcta aacacggtat taaaaattat
     2221 gatattgata tctatgccaa taatgaaagc caagcaaaga ctagttttga tgatgtattt
     2281 aaagtaatta aagatcatcc tgatttagat aaaaaagtat ttaaagctac gaaggaagtt
     2341 attcaaaata tcgctacaaa cagcaaactt cgttataaca cggcaaatgc tagaacaaaa
     2401 gatgggaagc gaccaggcgc aaaccgcttt gatgaaattc acgaaaatga agattattca
     2461 atgataaatg tggctacttc tggtggtggt aaaattcgag attatagaga attttatgat
     2521 acaactaatg gtcatgttcg tggtggtccg cttgatgaca ttatagaaga atcaaaaatg
     2581 attctttctg gagaacttgg aattgacaag gatggagcag aattttctag tttgtttcca
     2641 tttatttgtc gcttggataa cgataatgaa gttgatgatc ccgacatgtg ggaaaaagct
     2701 tgtccaacta ttaattacaa tgcagattta aaacggaaaa tgtttcaaga atactctcaa
     2761 atgcaacgta atgctggttt aagacttacg ttcatgacca aacgaatgaa cagacctatg
     2821 gaagatacac gatttgctgt tgcttcatat gatgatgttc tgcatacgaa agaaaaagaa
     2881 tttcctgaaa aaatggatga agtgatagga acagtcgatt ttgctgatag acgagatttt
     2941 gccagcgttg ggttgctagg aaaatacgat aaagatgttt attttacaca acatactttt
     3001 atccacgaat cagcccttcg attacaaaac atcaaacgag aggttataga tatttctata
     3061 gatcaaggaa aatcacagat cgttcatgga aaaaatatag aagctgatta tattgtaggt
     3121 tggtttcttg aaatgagtaa taaatattat attaaaaaaa tcgctatgga tatgtaccgt
     3181 gcaaaaatat tgaagcccgc tttagaagaa gcaggtttta ctgtggaaat tgttcgaagc
     3241 ggatctgtta cacatggtat gttaaaagat ctggttgatg acctttttat taatcaacgt
     3301 ttattttttg gtgacgatgc gattatgcgt tggtattgca tgaatgtata tgaagagcat
     3361 atttctaatg gaaatatacg ctatgaaaaa atagaacctg aaactagaaa aacggatggc
     3421 tttttttcat tccttcatgg tttgaatttt ttagatgata tttatgattc tgctcctgta
     3481 acagtcacaa atagctcagt agaaaataca ggaactggat ttactcctct agtattctaa
     3541 cttgaaagga ggtgagaaag tggggatttt tcaaaaggcg gtaggatact tcacaaaaaa
     3601 agcaacggtt cctttagaag aatacttttg taaattgcaa gttgattttg tgtatcgaaa
     3661 atttgcgatt gaaacttgta ttgatttgat tgcaaatgcg atgagtaaag cggaattcaa
     3721 gtcatatgaa gatggaaaaa ataaaaagaa tgatctttac tataggctga atgtagctcc
     3781 taataagaaa aataatgcaa cagaatttag aaaaaaactg atcaggagat taatattcta
     3841 caatgaagta ttgatcgttt ctccgtctaa taattctagc gaaatattta ttgcggatag
     3901 ttgggatgtc acagaatatg cattgaaaga tgatgtgttt tctcaagtgc aaattaacaa
     3961 catagtcctt gatagagaat ttctagaaag tgatgttatc tatataaaat acgcagatca
     4021 acaaattagg caactagtcg atgcgtatta tcaagcgtat gggaaactca tttctagtgc
     4081 catgaatgtt tacaagcgct ctaacgctcg tagatacgta ctgaaaggga atttattccg
     4141 accgcaagac aatacaacac aagatcaaat caataaaatg atgacatcac aatttaaggc
     4201 ttttatggaa gctgataatg caggtgcggt atttcaatta caaaatgagt acacattaga
     4261 agatttcagc ggaaacttcc aaagcaattc aagagatata aaaaacttaa tagacgacat
     4321 ctttgagatg acagcagcag cgtttcacgt tccgaaaaac ctactaaagg gagacatgag
     4381 tgggttatcg gatcaagtgg acgctttttt aatgttcgaa atcataccga ttgctgaact
     4441 tattcaggat gcgtttaacg ctagtctcta tgaagtagaa gaatacttgt cagggaattt
     4501 tgtacgtgtg gatacaacta tgatcaagat tactagcttc aaagatttgg ttgacgctat
     4561 tgatgtaggc attagaaatg gagtatttac aatcaacgaa ggaagagaac gcgttggaaa
     4621 tgatcgctct gataaggcga tggcagatga aatatttata actaaaaaca atcaacaagt
     4681 atcgaaagga ggtgaggcga atgacgacaa tgaaaacatt tctagcagta aagaatgaag
     4741 gcgcagtacc gcaaattttt attcagggat ttattggttc tagttggttc tttgaaggga
     4801 atactgacaa gggaatcaaa aatattttgg atagtctagg tgatcaagaa gaaattgaag
     4861 tagtaattaa ttcaaacggt ggagacgtat ttcaagggat tgctattggg aacttactta
     4921 agtcaaataa agcaaaagtt aacgttgtga ttaacggctt agccgctagt gctgcttcaa
     4981 ttatcgcaat ggctggcgat actataaaaa tctacaacaa tgcacaattg atgattcacc
     5041 gcgcttccac atacggagaa ggaaatgtcg atgacttccg tacgattgct gaccaactgg
     5101 aatcaattga taaatcggta aaggcttcat ataaaacacg attcaatggc acagatgaag
     5161 cattgcaaga acttcttgaa aaagaatcgt ttatggatgc agaaacagct ttgagttatg
     5221 gattggtcga tgaaattatc gatgcagaaa atagctcagg tactgaagct aaaaaagaac
     5281 aaagcgttga agaaattttg aatgacgtta aagaaaaaag agcagaaaaa attgctgcat
     5341 ttacagcagc attaaataaa acatttggac aaggagatgt aaaataatga cagttaaaaa
     5401 tttaaaaggt gtaacagctg caagcgacca attgatgaaa gcttttaaag atggtaacga
     5461 agaatctttt agcgcagcta tggtaagctt atctaaggaa attcaggata aaattttaga
     5521 agaagcaaca gcaaaaaatc aagatcaatt agtattaatg aaccgtggtc agcgtgtatt
     5581 aactacacaa gaaacaaaat tctataacga agtagtgaaa aacgaaggtt ttgcaggggt
     5641 cgaagaatta gtgccagcta ctgtatttga acgtgtattt gaagatttag aacaatctca
     5701 tccactattg caaaaaatta cttttgttaa cacaactggt gtaacagaat ggattgtgtc
     5761 acgtggagtc aatccagcat ggtggggtaa actttgcgaa gctgttaaaa aagttttaga
     5821 taatggcttt gatgtaatta acatgaagca gttcaagcta tcaggttata ttcctgtatg
     5881 taaggcaatg cttgatttag gtccagtatg gttagatcgt tatgtccgta ctgttttagt
     5941 agaatcattg agaattgcat tagaacaagc aattgttgat ggtactggta aagatatgcc
     6001 agtcggaatg atgcgtgaca tgagcaaaca aactagcgga gaatatgctg aaaaaaaagc
     6061 agaacctatt acagctttag atgctgtaac tatgggcggt ttgatggcgc gactatcaaa
     6121 attcaatatc gaaggtgtga atgatccgat ttatcgtaat gtgaatcctt ctgatgtggt
     6181 cctaattgtg aatccaactg attactggtc taaagtattc ccagctaaga ctgtactaac
     6241 tgctaatgga gaatacgtac aagtattgcc agtaccagtt tcagatttgc agtcaacggc
     6301 tgtgccagaa ggaaaagcag ttattggggt agcctcagat tacttcatgg gtgtaggatc
     6361 tacactaaaa attgaagctt cagatgaata ccattttgtt gaagacgaac gcatttatct
     6421 agctaaacaa tatgcaaacg gtcaacctaa acgtaacgat agtttcattg tattagatat
     6481 tagcgctttg ggaactacta ctacaactac aaaaccaaca accacaacaa ctacaacaca
     6541 agcgtaggtg atcagaatga agtatattct ttgtcagccg gcaatcactc ggtttaaatg
     6601 ggagcttgaa gtttgtttaa ctaatctgaa gaaactagga atcaaagata tcgtattgct
     6661 tttcagcaga cacgatgatc agattcctat tttttttgag aaggaatatg gtgttgaagt
     6721 tcatgtgtac gatgatctgc gggacgacaa agagtatatt ccttcgatta aaccatattt
     6781 atggtggaaa tatttagaag aagatcattc gcgtgaggac gaccgatatt tctatatcga
     6841 ttcggatgtc attttcaata aaagaattaa tttgcgcaaa ttgccttcta aagatgatgt
     6901 ttggtattgt agcgactgct gtagttatct aagtcttgat tatattagaa gctgtgaaaa
     6961 cggagaaaat attctaaaag atatggcaaa cattgtaaat gttacagtag aatctttgga
     7021 aactataaac actaattcag gaggcgcaca gtgggttatt aaccgtccta aagcgaatta
     7081 ttggaaaaag gtttatctgg attctaatcg gctatatcgc taccttagag ggcaaaaaac
     7141 aaatatacaa atctggacag ccgagatgtg ggcacagctt tggaacatga tgtatttcaa
     7201 tattggtcct aaagttcacg aggaattaga cttttgtttt gctactgatc caatagaaaa
     7261 agttaaagaa gtaaaaatct tacacaatgc tggagtaaca acaaacgatg aagatttatt
     7321 tttcaaaggg agatacgtga cttctacgcc ttttgatgaa gatttatcat ttgtaaacaa
     7381 gaaaaaatgc tcttacgcat atgttaaagc aattaaggcg gtggttagat gacgcctgaa
     7441 caagtgactg aagaattgct aatagctgtg aaggataata tttacgttac ctggaacgaa
     7501 gaagatgagt caattaaaaa gatgatagct aaaaatgctg tttatcttca aagtaaagtg
     7561 agtacaacac tttctttttc tcctgaaagc ttagaatacg gattgctaat cgaaagatgt
     7621 agatacgact ggaatcgtgc tttagatgag tttgaacaaa atttcgctag tgagttatta
     7681 ggtttcattc aacattatgc gctacaagaa tatattgcag gtgatgtgaa tggcgaataa
     7741 tcgtagactc gaagaaacat tcaacgatgg ttggttaaag attttgacgc aaactaccaa
     7801 aagaaatgaa ctaggaaaaa agattggtgt agaagataca gaaatcactt ctttaaaatt
     7861 tagaaatctt tccatgagag atagtgatat aacagctatg gatgcgatgg gatcgaaatt
     7921 aactaagaaa gtaaagactc catttcatcc aatcgccaag aaatttaata aagatcaata
     7981 ttttatcgta atcgatagta tgcgttacaa cgttatctat gccgattacg ataattttta
     8041 tatctatttt tatcttgaaa gtgtgggtga atatggtgat tgataattct aaagaaaaag
     8101 aacgtttaaa taagcaaatt tctgctatca aaacttcctt agaagagcat tttggcctca
     8161 aactctttca agactccgta ggcgaggatg agctacctga tgattttaat tacttcattc
     8221 tcgaaacagg agaaatagaa atgatcactg agccaaaata tagcgtgggt caaaatctat
     8281 atctaacttt ctattcagaa aatagagaag atttaacagg agactcacta gatattattt
     8341 cattgattca aaatcgttcg attcgttttc agagaatgga tcccaatcat ttaaaactag
     8401 agaaccaaga tcgctatatc gatcaattgg tatttacgtt tagacgatta ttgaagagtg
     8461 attgtcatgg ctaaaaatag ttgggagcta aaaataaatg gacatgatga acttcttgtg
     8521 cggatggaac gctattcaag cgagagcgaa cgactgatta acgaagcatt gaaatcaaaa
     8581 ggttcggcta ttgcagtgga taggattaca gaaaaaattc ctgtttctga agcagattta
     8641 agaagagggc accaacacgc aaaaaatagt cgtccactta agactcaata cattaatttg
     8701 ggtttcatca ttagacctac aagaaaattt gagtatttaa aatatcctga tttggggata
     8761 ggtacttcta aaagaaatca gccggacgaa tttatgagaa gaggattagg tcttgcactt
     8821 gatccaatta cagaacttct gattcgtcaa ttcgataaat taaataaata gggggaacaa
     8881 caatggctaa aacaaaaact gtagtaacaa cgttcgataa cgtaagtatc aaacgaattg
     8941 cttttaattt taagaacgca ggaaatgcaa tcgcaacgaa ttgtaatgga caattagatg
     9001 gcgaaacaga aatgcaaacg gtggttaaaa aatgtggagc gacagaagta aaatcaaaat
     9061 ctaaaccaat caatatgacg gtaacaatta ctgcacatgt accgatggaa gtttatcgac
     9121 gtttcaatgg gttgaaacaa gatggacgta ttaaaccagg catttactct tacggtcctg
     9181 attccgtagg cgaagatttc tcacttgctg cagagatcgt ggatgacttc gaagaaaata
     9241 gaaagttagt tggtatgtta gcatgcactt cgaatacagg attaacattc tctattgaaa
     9301 atggtgcgga tgaagtagct gcgttagaac tagaaacaaa agttatgcaa gatgaatttg
     9361 gtaaatttta tcatgaagca attgttgcag aacttgaaga agacttaaca gatcaatgga
     9421 tgacgaatct atctgctgat gtgattaaaa aaacttcagt tgtgacaact acggccactc
     9481 aatcacagta aaaaaaacgg aggtagcgaa atgaacgaag attactcaaa aattgaacta
     9541 aacgatggaa caattttgaa tttagaacct aaactgaata tcaagaaatt attgatgatc
     9601 aatagagatt ttaacacaga cgagtttgca aaaatgactg tgggaaaagg atccatggat
     9661 atttctgtta ttcaaggtgc aaaggctgtg tatattgctt accgccaagc gaacatgact
     9721 gattatattt cattcgatga atttatcgat aaatgggatt ttgatatggc tactgccagc
     9781 tatatttatc aattgatgat gttcaaacaa gcacgcgatg cttatcaaaa agaatttgaa
     9841 aaagcaaata aggaaaaaaa gcttcaaaag taaaaattcc aaagctctta gttgaaacgt
     9901 gggtcgatgt ctattcgatg ttgaccgacg ttttttctat gccttcagat ttggttttaa
     9961 gcgatatctg tttagatgac attttgcaaa tggcttacaa caagagtgct tatgaaggat
    10021 ggaaaaacta tgcaataaac caatcccaga aaaactaaag aaaggaggta aaaaatggct
    10081 aaaaagagaa cagaagcaga agtaactttc atagctaacg atgacggatt gaaatctacg
    10141 ttaaaagaaa tcagtgctga attaactaaa aatagagcag aattaaaact agaacaagct
    10201 caattacaac agactggttc tgaatcagac aagttaggaa gtaaattatc ttctttagaa
    10261 aagcagtatg aattacaaag tcaaaaagtt gaagtaacta gccaacgttt agccaatgcg
    10321 aaaaaatatt atggagaaaa ttccaccgaa gttcagaaac ttgaaagaga actgattaat
    10381 caacaaacag cgcaacaacg tttgtcaaac gaaattgata aaacgagtaa tgcactagct
    10441 caagcaaaag gcgaaataca gacgtacgag tctacaatgc aacagttgga tagcgaacaa
    10501 aaaaatgttc aagctagtgc ttctctgatt gaatccgaat acaaaaaatg gcaagcaact
    10561 gctggtcaat cagcttctga agccgagaaa ttagcgaaag cccaagaata tgtttctcaa
    10621 caatctgaaa atgcggagaa aacgatagat atcctaagac gacagttaga agctacacag
    10681 tctgaatttg gcgctacatc cacagaagca atgcagatgg aagcgaagct taatgatgct
    10741 gaacgtgaat ttgaagagtt aggacaagct gctaaaaatg tagatacaac taacttggac
    10801 gatatcggaa gcaaaataga tatgaataat ttaatggaag cttctgacgt tttaagcgac
    10861 attggcgata agcttacaga attagggaaa caagcagtgg actctgctaa cagtgtaggt
    10921 agttcccaga gtaagataca agctaatttt ggtttgtcta aacaagaggc tgaagaatta
    10981 acgaatgtag ccagagacat ttattataaa ggttttggag aatcgttaga tcagtccaca
    11041 gatgcattga ttttggtaaa gcgtaattta ggagatttaa ataatcaaga tttacaaaat
    11101 atcacggaac aagctatggt cctagaaaac accatgggcg ctgatatgga tgaaacgtta
    11161 cgtggtgtaa atggcttaat ggtcaatttc ggcttgagtg ctcaagatgc aatggattta
    11221 atggtttcgg gtactcaaaa cggtttagat aaaacgcacg aattaggcga caatatggca
    11281 gaatatagcc aattatggag tcaaatggga tattcagctg atgaaacgtt cggaatgctt
    11341 caaaatggtt tagatgcggg tgcttataac cttgataaag tcaatgactt agttaaggaa
    11401 atgggaatat cgttaacaga tggtcgattt gagcaaaaca tggatatgtt tagtgaaagt
    11461 actagaaaag cttttgaaga gtggaaaaat ggcggaggaa cacaaaaaga cgttattaat
    11521 tccatgattc aagattttag caatatggat ggtcaatacg accaattaaa taaagcttcg
    11581 acaatttggt ctgcgcttgg cgaagataat gcgatgaaag ttgtccaatc tttaactgat
    11641 gttaaccata catttgatga tgttagtgga tctgcacaaa aaatgaatga agattctact
    11701 actccgttgc aagagttgaa cgggaaaata gctgaattaa aggattcatt agctcctata
    11761 ggcaacacaa tcatagatgc actcgaacca gtaattgatt ttctaggaaa gatggctgat
    11821 gcgtttaata atcttccaca accagtacag gatttcattg ttgctatagg tggtcttaca
    11881 gcagcatttg gcatactgtt accggcaata ctagccgtgt catatttatt tggtccgatg
    11941 atgcttataa ttggaggaat tatagctgtt atagcaggcg ttattgtggc aattaagaac
    12001 tggggtgcaa ttactgactg gtttagtggt ttatggaaaa aatttactga ttggttgggt
    12061 gatacttggg aaagtataaa agacggagcc tcatcagttt gggatggggt taaagaaacc
    12121 tggtctggat ttgtagattg ggttcaagat atttggcaag gagtttctga ttggtttgga
    12181 gagctatgga gcggattagt tgaaggagct tccaacatct ggcaaggagt ccaagaaact
    12241 tggcaaacat tcgttgattg ggtttcaaat atttggaacg gagtcaaaga agtatggtcg
    12301 attatttggg cagacattgt aggaattgtt caaataccat ggacattaat aacgtcattg
    12361 attcaagctg gtattaatat tatcgtgggt atttttgatg tagctggaca gttattgggc
    12421 gcagcttggc aagctgtttg gacacctatt tctgatttcc ttaaaaatac ttgggatact
    12481 atgacacaat ggataagtat cgcttggaat gggattgtaa ctacattcca tactatattt
    12541 gatccagtag tggcatggtg gaatggtata tggacagcta ttagtactac ggcttcaaat
    12601 atttggaatt caattagtgc aacagcttct agtatttgga acagtatcaa gaatacaatc
    12661 actagcttgg tacaagcagc tgctacagta attcaaaata tttggtcaac tgtatctagt
    12721 tggttaggtg gaatttggaa ttcaatcagc tctacagcat caaatatctg gaatagtgtg
    12781 actagtagta taagcaatgc tataaacgca gctaaaagtg ccattcaaag tgtttggaat
    12841 agtatatctt cgtggattag cggaatttgg aacggtatca aaaatactgc tttgaatctt
    12901 tggaatggaa ttacaagcac tattagctct aaagtaaacg atggaaaaaa tgcaatttca
    12961 agcggttggt ccaatctaac aggtattgtt tccgacatat tcaataatgt taaaagtaca
    13021 attgctaaca tatgggaagg catcaaaaag actgttagcg ctccaattga ttggattaga
    13081 gacaaaatca gtggcatttt tgataatttg aatatttcta taccacatat tccgttaccg
    13141 gaatttatca tggaaggcag ctttaacccg ctaaaaggtc aaatccccca tttgcgtgta
    13201 aaatggcatg ctaaaggagg tatctttact aaaccaactt tactaggtgg aatgaacggt
    13261 gtcggtgaag caggacctga agcagtttta cctttgaaaa gatctgtttt gcaagaaatt
    13321 ggtgatcgta tcttgagtag cacatcagtt tcatctaggg cacaaacgat tcaacctgtg
    13381 aataactacg aattcaattt cacaattgat ggtaacgcag atgaggttac tatgaagcaa
    13441 acaactcaac aaatcattga tagcattaca aaaattcaaa atgataatgc ttcggcatgg
    13501 cgttaaacag gagagtattt ctcctgtttt tttagtatta aaaaggatgt gaaaaaatga
    13561 ctgattgtat acattctata atcgatggat ttcctgatta tttgcataaa ttggctttag
    13621 ctgaaagacc aaccatacct tctccaaaaa gacagagagt tgaaacttct gttttaggta
    13681 ggttaggtgg cttagtacaa gattactcgt ttgaagatat gtcgtttaca ttgcactata
    13741 actatttaga ggatgtggaa gaccatcaag cgttcaagca atcgttttat atcatgcgtc
    13801 attggttaaa ctatgcaaag aaattagaat tctctgacga tcccaacgtc tattacgtta
    13861 tccagactat cgatattggg gatgcagaaa acgatattgt tgaatgggga gagttcgatg
    13921 taaatattac tgcgaaacca ttcgcaagag ttcaagaaga tgtacctata accgtagata
    13981 aaccacagtc atttaacttg ctgaataata gtttagaaga aagttttcca aagattatca
    14041 tcactccttc agctacttca tgccagttca tcttaaatga ttatgtgttt agtttcgaag
    14101 gcttagtagc aggaactgat gtagtcattg atagtgattt gatgctttgc tacgaagagc
    14161 aatcggacgg agatatttta gatcggtcca acaaaatgaa gaccatgcaa tatccgacat
    14221 tgcaagtgga tatcaatcat tttaattgca ctggtttgag caaaatacaa atttatcgta
    14281 acgggttaag gtaggtgaaa tagatgatcg ataatttaat aactatttac gataaaaatg
    14341 acgcgaataa tttagctgaa catttatatg atacgcaagg tttaggcgct ttgtcagact
    14401 ggttaacagc tactgttagc aataaactaa acggagccga gatatttcag ggtacttatt
    14461 caataaacgg aactaatgca gatttgattg tagacggacg tattattcag tgttatgtag
    14521 atgaaaatcg agcaaaacag cgtctacgga tttattatgc aaaaacttct gtaataggca
    14581 atacgataga agtaaaagct gaacctattt tcaatgatat aagaaaatcg gtgttgaata
    14641 aatatgacag cggaacagaa aagatcactg ctactcaggc atggcaaaac gcaaaagttt
    14701 tagcgaaacc agctatccct tcgcagtttt ctttctcgtc attagtagat acgcttgcta
    14761 atgtgaagat agaaaaggcg aattttttag aattctttgg tggaaaagag ggatctattc
    14821 tagatcgatt tcatggtgag tttctaaaag ataataacac attacgtcat gaaaaaagtc
    14881 taggtacgga tcataaaatc aaagcgattt atactaaaaa cttaactggt cttgacttag
    14941 agattgatgc tcaaagtgtt ttagttggag tttatccatt cattagcagc tcttcagaag
    15001 gagaagacga gatcactcta ccagaagaag ttattttcac ggattacgtg gatgattatc
    15061 ctgctggata tgtttctttt gttgatttta aagacaaagc gactgatgta gccacattaa
    15121 gggaagctgc taaagactgg ttgaaaacaa acatagataa acaaaaacca caagtgagtg
    15181 gttcgattga attagtacca ttgaggcatc aaagaggcta tgaaaaattt gttgatctag
    15241 aaaaagtttc gatgggtgac ggagtagatg tgtatcatcc acagttaaaa gtgaatatgt
    15301 cagcgagaat tgtggaatat acgtttaatg ttctaaccaa ttcatacgat aaattagttg
    15361 taggaaacgt caaaacaaac ttcttagaaa acacagagaa taatgtcagc aatttgatta
    15421 atgatgccat tgatcaattg aaaaacggtg gcgaaatcag tgatttactc aatgatattg
    15481 tagatcatca aactgatatg attactggtc aaaatggtgg ttatgtttta ttagatccta
    15541 aagaagcgcc tagtcgtatt ttgattatgg acacaccaga taagaatacc gcaaggaatg
    15601 ttttacaaat caacaatgct ggtattggtt tttctaaaac tggcattaat ggaacatatg
    15661 aaacggcatg gacgttagat ggcggattca atgcctcgtt tattacggct ggtgaaatag
    15721 taggaattac tattagaggt actacattaa ttagtgatgg tgctgattat agaacaagta
    15781 ttgctaatgg caaaatgact tggtactcaa aaaaagttaa caaagatatt atggagctag
    15841 aagcacgtga ttatgtaagt gctgatgccg gtattgtatc atacaccatg aaaactggtg
    15901 gtggtttcat gattagaaat ccacagggta acttggtttt tagtacgtgg gataatggta
    15961 ataacagacc gtttctatct tttggtgcgc ccaatttcag gtatagcaat gctagttatg
    16021 taacttctgg cgacggtagt tctttaagca ttaatggtag tgcgggtaac tcatgggaat
    16081 ttaaggtagc tggtaggact atgaaattta ctagtgatgg tatgctaacg ttaccaggtt
    16141 gtttttttgg ttcatgggaa gatgggaaac ttgctaggtt tgaacaatca acggtacaag
    16201 tatataaaga ttttactgtt agaggtacta aaaactcaac tgtaccaaca gaacattatg
    16261 gacaacgact attgaacgct tatgaaactc cagaatatta tttcgctgat tatggggaag
    16321 ccgttacagg tgacaatggt aaagttcgtg ttgatattga ccccatgttt gctgaaacag
    16381 taaatctaag tcggtatatg acacatgtga cacctacaga actagttttg tgtgctgtta
    16441 ctcatgaaga tattgaccat ttcatcattg aaactagtaa gccaaacgta ttagttagat
    16501 ggaatttagt ggcacaccgt ctagggtatg aagatattag attaaaagag gatacagcat
    16561 atgatagcac agtgcttgac caaaaacgtt tttaaaacga agacaaggag gtatataaat
    16621 ggctagcagt ttatataatt tggctttaga tttcagcaaa gaattaaact acaccaaagc
    16681 tattatggct cgtcaaggtg ataaagggat tacggtgacg gttaaaccgt ttctaaatgg
    16741 cttgcagatg gatacgagtg gcggaacatt tactttaaaa ggaacaacac catctaaccg
    16801 ttacgtagat aatgttgcaa ctagtgtaac tagtgaagaa gtcacgtttt ctcttgatgg
    16861 cacatttatg agtgaagcag gatattataa acactgctac gtagaatata gaaaagacaa
    16921 tcaaatttta acaacgcaag atatcatttt tttctcacta ggagtgtctg acatttcgca
    16981 aggccaagcc gatgaatatg tttcgcaatt agaagagttg attcgaaagt acaacgaaac
    17041 ttttgatgct tttatggctg aaatcaaagg tagagtggat agcttaaatc aacagattac
    17101 tgatttaact ggtcaagcta aaacgctaca agacaagtta gatgctctga aagaagaaat
    17161 ttctaagtta ggtaacttac aagtgatgta cagtaacagc atcgacttcg gggactatga
    17221 ttattcggag aacccgaatt taatgccata tattacagaa ccttgggttg gacctttgtt
    17281 aggtaatgga catacagtta aagatagtgt taaaagggtt ataactcata ctaaaactag
    17341 aacagctaat tccggcgaca tattatcgct tggcttaggt attccgtgta cagcggaagc
    17401 taataatagg tatcttataa ccactttacg accatctacg acatacacat taagcgttac
    17461 tatgagtgtc ggttcagatt ggactggaga gaccaatact ataggggtta ggcttagata
    17521 tttaaatgaa caaggtggta tagaactgcc cattaatgca ttaataccag caaatgtaga
    17581 acgcgataaa atggtcactc atacatttac tggtataaca aaagataatg taactagcat
    17641 aacaaactgt tatgttgaaa tattctcact taatagtgaa tataaaggaa cggttagtgt
    17701 cagctatgac gttaagttaa aagcacacta ccctaactta ctagatggcc cttactggtt
    17761 aggtaaagtt cctttaggcg agaatattgc tgaccctaca gttgtctttc cacacaaaac
    17821 tagtgaatat atggtttatg gtagacgcaa tacagaaaac tatatcgctg accaaacata
    17881 taccatatca atgaaagcaa ctaaactaac agttcaatcg tttgccgtgt atattgctgc
    17941 tggccgcgtt aaagtaggag acatgaagcc tactgagggg ttagctaata catgggagtt
    18001 aacttttact gtaactaagc aacatataga tagtggggta actaattatc tggaaatata
    18061 ccaatacccg tcagctacaa aaggagctgt tcaaattgag tggctcaaac tagaaaaagg
    18121 caatactcga accccgaata ttagtgagta taaatatcgt ggtactggta tgcgtgattc
    18181 aaacaatcca aaagattatg tttgggatct agcaccagaa tatgtcgaag ataatttggc
    18241 cacagatatt aaaatttctg aaattactgg taaagcaaac aattataccg atgggaaagt
    18301 atcggagatt aattcgcagt tgactgcttc aattaatgaa gtagacacca cagctaagga
    18361 tgctcaaaca aaagcgaatg ctaatgcgac tgctatagat gaattagaca ataagatcga
    18421 tgaacgcatt aatgatacag ctactaccac attaacagtt acaaacggga ataccggatc
    18481 agcaaagctt tatcgtgaag gaaaaacagt ttctatatat tttgtggctt taaacggaaa
    18541 aagcagtggt ggaaatgatt caacgatact aacaattcca gaaggctatc ggccaccaat
    18601 tagttttgag caactggttg gctcgataga ccgttctact ttgaacagtg ctcagttatc
    18661 tattggtgca gatggagcca ttaaatggcg aagaaactca agttatggat cggattatac
    18721 ctttgcaatt acttacacga tttagaaaag cgtgaatcga tatgaaggca gcatatagac
    18781 caattgaacc ttacggattc gagcaaatca ttgtgaatga tgaagaacat ttaccggaag
    18841 aatgcacaga agtcgaacca ccgattccaa attggaaacc gaaattcaat tactgggagg
    18901 gaaataaatg aaaaacattt ggaaatatgg acgtactggc ggagagtacg caggaaaagt
    18961 attggacgac atgcttgtat ccgttcctta cacagatcag ccaccgcttg aaggaattcg
    19021 tgctgatggc gaaccgttaa caatcgctga tcaaatgttt gatcctaaat tgaatcaatg
    19081 gattatttta gcgaacgcac tagatcacaa cgatttaaac aatctcaaag caatgtatga
    19141 gtcgttagaa aatgagaacg gcgatttaaa acagatcaat gccaaactca tgctaagcga
    19201 tgtagcaatt aaacaggaaa atactgcatt gaaagaaaaa gcggatagtt tagcacaaat
    19261 caattcaaaa atgatgcttg cttcgttaca aaatagcaaa gacatttcag aaattaaaga
    19321 gcaactaaat ccagcttcaa agggaggtga gtagtatgtt tagttttagc gatgtgaaaa
    19381 tgatgtatga ttggggctgt ttcactaacg aacaagtaat ggttttcgtt ccgttgtgca
    19441 ttactgaaga aaaagcagat aaaatcatta gcaaagaaga gagcgcatct taattgatgt
    19501 gctttttatt ttgattcaag gagttgtcac atgattaatt taggggaatg gggagcgata
    19561 gcaggatcaa taaccgctat cgtttctttg attttattag taataaaacc aattactgca
    19621 tctttctcga agattactga gactctttca aaagtaagtc acaatttaga tttgctgact
    19681 aaagatttag aatcgagcaa atcagatcga ttgatgattc atgaagaact aaagaaacac
    19741 gatgaaagat tagatacaca tgcagaaaaa ttggtagaac acacgcaaca aattaaaact
    19801 ttatttaggg agagaagaaa atgaataata aaacgttcga agtactaaaa tggttcgcac
    19861 tggtaattat tcccgcacta gctactttcg tggggttagt tggtaaagcg ctcaattggc
    19921 agtacacaga tatctgtgtt gtcatcatta ctggttttgg cgcgttttta gggagtgtgt
    19981 tgggtgtatc aaatcgaacc tacaaaatgt tctcggctga aagcgaagaa ggaggaaaca
    20041 aatgaaaaag aaaattacta ttactgcgat gagcctgtta acggctcttt ttttattacc
    20101 cattaatacg tttgcttata ctattaatga cgagtataat ttagcgccga atcaaggaga
    20161 ctccagatta gcaattccta acaaaattat tttgcacgaa actggaatag atgcaccagc
    20221 aagaaacgta gccgccaaca tgaaaaataa ttataacgga agcaattctt atactacaga
    20281 tgttattggt gacggtggga ttgtttaccg tgtgggtgag caaggatatg tttcgtgggg
    20341 agctggtaac gctaatcctt atgcgcctgt acagattgaa ttacagcgca catatgataa
    20401 agcattgttt gaaaaaaact atcgagctta tattgaatat acaagagata gtgcaaaaaa
    20461 atatggaatt ccattgactc ttgatcaagg aacttcttta tttacaaaag gaatcatttc
    20521 tcatttgtgg gtgacaaatt atgtttgggg gaaccacaca gatccatatg gttacttatc
    20581 gcaaatggga gttagcaaag aaaagcttgc ttatgattta gctcatggat ttaccgatga
    20641 aaatccaaca acttctgaaa acaagcctgt cattgatcca acacgagctg gtgcagctaa
    20701 tcctacgctg acagatggaa caaatcacgc ccacattgat cagtttgggg aaatcgaaaa
    20761 cgcaaacttg catgtggctg gatggcacat tgctaactat aaatacgagt atattttcat
    20821 tatggactac aatactggga aagaattagc tcgagtaaga gctgatggaa tttatagacc
    20881 agatgtaaac caagcttata atacttctgg aaatgttggt tatcatgtat ctttcaatat
    20941 gcgtaatttt cctagtaaga aagtctatgt aatgatgcgg gcaacgaatg atccagaggg
    21001 aaacactaaa ggcggtgcgc aagatttcca tgacaaac
//
LOCUS       DAKBVG010000024        20912 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000024 DAKBVG010000000
VERSION     DAKBVG010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 20912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.024"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(62..1435)
                     /locus_tag="OUD09_000692"
     CDS             complement(62..1435)
                     /locus_tag="OUD09_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCT9691987.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPIMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(1435..1830)
                     /gene="rbsD"
                     /locus_tag="OUD09_000693"
     CDS             complement(1435..1830)
                     /gene="rbsD"
                     /locus_tag="OUD09_000693"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCT9691988.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGSEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(1858..2775)
                     /gene="rbsK"
                     /locus_tag="OUD09_000694"
     CDS             complement(1858..2775)
                     /gene="rbsK"
                     /locus_tag="OUD09_000694"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCT9691989.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(2884..3897)
                     /locus_tag="OUD09_000695"
     CDS             complement(2884..3897)
                     /locus_tag="OUD09_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCT9691990.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQISHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEESGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(3978..4580)
                     /locus_tag="OUD09_000696"
     CDS             complement(3978..4580)
                     /locus_tag="OUD09_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCT9691991.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKGIVNNGTLSPHTAQSILTNN
                     DLLAKTLKRTAALKVLVNVDITTFSYLSHQTDAGGISALSAPAEFTDLLLKDSLKDTN
                     NMLTITKYNPSAFFGTSLDLQLRRRGIETIVLSGVATTNGVYATALDAFQHGYHVVLA
                     EDACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            4765..5466
                     /locus_tag="OUD09_000697"
     CDS             4765..5466
                     /locus_tag="OUD09_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT9691992.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            5476..8187
                     /locus_tag="OUD09_000698"
     CDS             5476..8187
                     /locus_tag="OUD09_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT9691993.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEHNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKITEGKAQIAQGRTQ
                     LEDAKKQLAQQSAAIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKAAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWSYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            8584..10212
                     /gene="treC"
                     /locus_tag="OUD09_000699"
     CDS             8584..10212
                     /gene="treC"
                     /locus_tag="OUD09_000699"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCT9691994.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRITIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(10272..11453)
                     /locus_tag="OUD09_000700"
     CDS             complement(10272..11453)
                     /locus_tag="OUD09_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCT9691995.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSVW
                     LVPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            11705..13231
                     /locus_tag="OUD09_000701"
                     /pseudo
     CDS             11705..13231
                     /locus_tag="OUD09_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
     gene            complement(13300..14028)
                     /locus_tag="OUD09_000702"
     CDS             complement(13300..14028)
                     /locus_tag="OUD09_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCT9691996.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(14041..15321)
                     /locus_tag="OUD09_000703"
     CDS             complement(14041..15321)
                     /locus_tag="OUD09_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HCT9691997.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIALSAFLSALLAFCFKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYAQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLVVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(15462..16064)
                     /locus_tag="OUD09_000704"
     CDS             complement(15462..16064)
                     /locus_tag="OUD09_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HCT9691998.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            16310..16516
                     /locus_tag="OUD09_000705"
     CDS             16310..16516
                     /locus_tag="OUD09_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9691999.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            16829..17548
                     /locus_tag="OUD09_000706"
     CDS             16829..17548
                     /locus_tag="OUD09_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HCT9692000.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(17629..18312)
                     /locus_tag="OUD09_000707"
     CDS             complement(17629..18312)
                     /locus_tag="OUD09_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HCT9692001.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            18544..20730
                     /locus_tag="OUD09_000708"
     CDS             18544..20730
                     /locus_tag="OUD09_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCT9692002.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         7147 a         3747 c         4067 g         5951 t
ORIGIN      
        1 cctaaaaaga aaaagactga aatatgtgat aaacatctca tacttcagtc tttttcatta
       61 actattcata agacattagg tcttcatcaa acccgctaac gagagtagta atcattgcta
      121 cgactagact aacagccagt cctgctaagt accagaaaaa agtaccggaa atgaatgcag
      181 gcaatgtggt cagactacca aatacaaaag cgatcgcttt cacttgggca aatcccatta
      241 cagctccacc agcagctgca ccaatgcaag cagcaacaaa tggttttttc aatgggatcg
      301 caattccgta aacagttggt tcggtcactc ccgcaagtaa aatcgaagca aaagcagaac
      361 cggagatcga acgagttttt gaacgtttac ttcgtagaaa tacacctaaa gctgcaccag
      421 ccattccaaa attggctgct cctgtcaacg gaacgattcg gtcgaaccca taagcagaga
      481 tattactgat catgataggg tttacagccc attggatacc aaatattacc aaaatattcc
      541 aaccaccagc gatgattgcg ccgctgatcg gtccgttaat atccatcatc caagtaattg
      601 cggcagctaa accttcgcca aggtaaacac ccgcaggccc gaataccatt aacgtcaaag
      661 gaaccgtgat aactaatgaa agtaaaggta caaatactaa ttgcatattt tcgcgaatat
      721 attttttgag gaatttttca acaaaagaat aaaagaaaat acctaaaatc gccggcatga
      781 cagtagacgt atactgcatc agtactacag gaatccccag aaaatcagta gctgctttgc
      841 cactgtctga aagtaaagag gtcagattcg gttcaatcag tgctgcagca atgacagccg
      901 caatatatcc attcacttta aacgttttgg cggcagaaaa agcaagaaag attggcagaa
      961 aataaaagac ggcattcgac gcagcagaca aaatatgata agttccgctt gtttcactaa
     1021 gccagccaat cgtagtcgct aaaacaacca gaccttttaa tataccagaa ccggcaaaca
     1081 gcggaatgat cggcgtaaag atttccgata tcaaaccaaa tagtttttcg atcggattta
     1141 gcttttcttt aggagctgaa tgatcgattt gacgcgttaa ttccttgtaa gcaggagcca
     1201 atccctgatc catgattatt tctgcatatc tatcgagtaa aatgaaatac ttaatggaag
     1261 ggatttgctg ttttaatttt tctgcattgg ctactggctg ttttttcaat ttgatacgaa
     1321 gccgagtcgc acatggaagg acagaatcaa tattgtcttt tcctcccaac tgttcaatca
     1381 gcatatgaat ctgctcggac agtaccatct gttcaggatt cattgtcgtt tccattagaa
     1441 gaccactcca ctcactaata taatattggc gtaaggtgtc atttcgcctg tacgaataaa
     1501 tgcatgacag ttattcaact cttttttcat ttcttcatgt gggataaagc taatcggtgt
     1561 atctggtaac aataatttga ttttttccaa tatttccggg ttcattgtct ttatttcttc
     1621 cgccaattca atcgattgta cctcaagttc ttctaaaacg ttttctaaaa cagataaaaa
     1681 gcttggaaga ccgtttgaga cagccaaatc tattttttca ctaccaaagg ggacaggcat
     1741 gcctgcatcc ccgatactta atttatcaaa gtgccccatt tgtgcgatta ctcgtgaaat
     1801 atctgaattg attacttttc cttttttcat gtttgtttct tcctctcatt gttaatttta
     1861 gactgctgat tgttctaaag cttcgcggta agggatagaa ggttgcgcac cataagactg
     1921 cacagcaaca gaagaggcta gatttccata aaggatcgct tctttcaagt tactaaggtc
     1981 tggctgaagt gtgctgctta atgcgccaat aaatgtatca cctgcggctg ttgtgtcaac
     2041 ggcatccact ttaaaagcag ggacgatacc gtgttctttt tctgtatgat agaaagcgcc
     2101 ggcactgcct agtgtaatga ttactgttcc aatacctaat tcatggagtt tttcggctgc
     2161 tgcgaccaaa ctgttatgat ctgtcactct gattcctgta atgatttctg tttctgtttc
     2221 atttggtata atgatatctg ttttttcaag tagttcagta gggatcgttt cacgagctgg
     2281 tgcaggattc aggatcgttg tttttcctgc gtcttttgca atagaaaaag ctaggatcgt
     2341 cgcatctaag ctactttcaa actgagcaat aataaagtca ctgtttgtaa tcagttgctt
     2401 gttttttagg acttcttgtt catggaaagc attatttgca cctgcatgga tcaagatact
     2461 attttctcct gctgcgtcta ccataatcat tgcttgtccg gttgtggcgc cttggatttc
     2521 agctatccca gatgtatcga tgttttcttt tgtcagtaga tctaataact gttgtccttc
     2581 gctgtcagcc ccaacgcctc caataaaaga tgtccgtgcg cctgaacgtt tggctgccac
     2641 tgcttgattg gcacctttac ctccgccagc gtgaaaaatc tcatgcgcat gcattgtttc
     2701 gcctggcttg ggcatgttgg ttaaacgaag ggtagtgtcc atattaatac ttccaattac
     2761 tgtaaccgtg ttcattttac aaacctccaa ctatctcatt tattttgatg ggaaaaatta
     2821 ttttttaaaa cgttttagta aaacgtttta atagttcgat aaaaatatag cacaccttga
     2881 aagctatttc aagggtgcag ttgaaaatct tttttctaat cgtacaggta atcttttttc
     2941 ttcccattct ttttctggga attggatccg atctaacaat aatttggcag atgtttgtcc
     3001 caattcgaag atcggttgtg cgatcgttgt cagtttcggc ttgatatact cgcacatatc
     3061 tatattatca tagccaataa tgctgtagtc ttcaggaatg ctttttccag attcctctaa
     3121 tccgcgatat aatccaaaag ctaattcatc atttaaagca aacacaccag ttgcagcaga
     3181 atcaagaagt tctgctgtga tttggtagcc accttgtttt gagaactgtg taggtaagag
     3241 gatcagttgg tcgtgagaga tttggtaaac atcaagagca gatttaaacc cctcaatccg
     3301 agcgtggaca ttttccggcg gattttcagg atagacaagg gcgatcgttt gatgacctag
     3361 actaagcaag tgcatgcccg ctaggtaacc accgcgaaaa tcgtctgttc gtacggcatc
     3421 actaaatcct tcagattttt tctgatccaa aacaatgaag ggccggcctt gttttttcaa
     3481 attttcgttg attgcatctg tagagacagc agaagtcgcg ataatgaatc catccacacc
     3541 tcgacgtgtg agctcggcta gatattcgat ttctttttga tgatcagaat cagcattaca
     3601 taatatcgtg acgaaattct gcttatacaa gatatcctca attcctcgca tcagtgtact
     3661 gaagaatgga ttcgtgatat caggaacgag tacaccaatt gttttcgttt ccttggtaat
     3721 catttgtcta gcaaaatagt caggctgata gcctaattct tctttggcag ctaaaacttt
     3781 ctgaacagtt ttcggactga atttctccgt atttccattt aatatcaagg agacagttgt
     3841 gattgaaacg ccggaaagtt tagcgacttc tttgattgtg atcttttttt tcaccatgtt
     3901 catactccta tctaaatcct gtggttattt tcccatgaaa tcaaaaagaa gtaaatgaaa
     3961 atcacgtgtg ccagtactta tttgctttgt tggatagctt cgattatttg tttcgttgag
     4021 cgtactcgtg cagttttcgg aaatatcttt tttatgaaca attgatggga ttctttgtca
     4081 cgatcactgc aagcatcttc agcaagtaca acatgatacc catgttggaa ggcatctaat
     4141 gcagtagcat atacaccgtt tgttgtcgct acaccgctta aaacgatagt ttcgattcca
     4201 cgccggcgaa gttgaagatc aagagaagta ccaaaaaagg cacttggatt gtattttgtg
     4261 atagtcaaca tattgtttgt atctttcaag gaatccttca acaacaagtc agtgaattct
     4321 gccggagcag acaaagcaga tattcctcca gcatctgtct gatgacttag ataagagaaa
     4381 gtagtgatgt ctacattcac taaaactttc aaggcagcgg tacgtttcaa tgtttttgct
     4441 aacaaatcat tgtttgtcag aatagattgt gcagtgtgtg gagagagtgt accgttattt
     4501 acgatccctt tttgcatgtc gatcgcgaca aaagcagttt ttgataaatc aaaaatcata
     4561 ttttccttta aatcattcat gatgttcctt ctttcaaata acaatgtttt ttatgtaagt
     4621 aaatcataaa tcgtttattt gtcaactaaa taaaggattc ctttctttag tctagagaaa
     4681 ttcagtggta aaataaataa gtatgagaaa acgcattcga tgcatagata tagcgagaaa
     4741 tatcgtgaaa atggaggaat cagagtgaag tatgtcgaag taaaaaatga atacaaacga
     4801 tacaaaatgg gagaaacgac cattacagca aatgacgata tttctttttc aattgaaaaa
     4861 ggcgaattag tgattatact tggtccaagt ggagcaggaa agtcaacgat tcttaatatt
     4921 ttaggtggga tggatacacc tgatgaaggt cagatcatta ttgatgacac agatattgcg
     4981 caattttcag ataaacagct tactgcttac cgacgaacag atgtagggtt cgttttccaa
     5041 ttctacaatc tcgttcctaa tctaacagca aaagaaaatg tggaattggc aacggaagtt
     5101 tctcctgatg cgcttgatcc tgtagaggta ttgaggcaag tgggcctagc tcatcgattg
     5161 aataactttc cgtcccaatt atcaggaggc gaacagcaga gggtttcaat tgctcgtgca
     5221 ctagctaaaa atcccaaact tcttttatgt gatgaaccga caggcgcgct ggattttgaa
     5281 acgggcaagc aagtattgaa attattgcaa aacgctagca gacaacacgg caataccgtt
     5341 ttgatcatta cacataattc tgctttagcg cccatcgctg atagagtcat tcacattaat
     5401 gatgctaaag taaggtctgt tgaattaaat gatcatcctt catccattga tgagattgta
     5461 tggtaggggg attttatgaa aacaagaaca tatcttaaag caggttttcg cgaaattcgg
     5521 caatctaaag gacgattcat tgcaatcatc cttattgtca tgctaggaac acttttattt
     5581 gtaggtgtca aaactgctgg acctgtcatg caaaaaacga tggaccacta tgttggtaca
     5641 gcaggacttt ctgatttgca aatcgtttct actggtggct tgacacaaaa agatattgct
     5701 caagcagaaa agatcccaga tgcaaaagta gaaacaggaa aacagatcta ttattccaat
     5761 actggtaaaa acgaagtgat tcaaatattt tcttataaca aaaataacaa gcagaacaag
     5821 ctacaattaa ctgatggaaa actcccggaa aaaccaaacc agcttgtatt ggatgaaaaa
     5881 gcggaagatg aagggtataa aatcggtgat atttatcaga ttgattcaga tgaattgaaa
     5941 gaaaaagagt ataccatcac gggctttgtc cgttctccat tgttcatcaa caatttggag
     6001 agaggatacg cgaacgtcgg aaatggtagc gtcgattatt ttgtctattt acctgaatcc
     6061 gtttttaaaa cagatattta ttctgtctta tatcttgatt ttacaaacgt aaagaatttg
     6121 aatacgtaca gcaaagctta caaagataaa atggaacaca atcaagagaa ggcagaaaag
     6181 tatttacaag atcgccctga agaacgctta gaagagctga aaaaatcggc aaacgaagaa
     6241 ttagaaccag cacagcaaaa aataacagaa ggcaaagcac aaatagcaca gggaagaaca
     6301 cagttagaag atgctaaaaa acaattagcg caacagtcag cagctatcca gcagcttcct
     6361 gaagcgcaac gaacaatcgc tgttcaaaca ttatctcaac aagaagctca acttgctgaa
     6421 caagaaaggc aattagttga gaaagaacgt gaactggcct ctgcagagga agaattgaat
     6481 cgaaatgcag aaaaaattga aaatttaact aaaccatcat acctatacaa tgaacgttca
     6541 gaaaatgtcg gttttcaaga atttggtgat ctgtctgatc gtatcgctgc gattgccaat
     6601 gtttttcctg ttttcttttt ctttattgcc gcattgatca cctttacgac aatgacacgt
     6661 atggtagaag aaaaccgcag ggaaatcggg acattaaagg cgttaggtta tacgaaagtt
     6721 gaaatcgcaa aaaaatatgc aatctatgca agtctagcct cagggatagg cattgtttta
     6781 ggaacgatat taggaacgaa tcttttgcca aggatcattt ttgagctgtc taacgagcgg
     6841 tatgatattg ggagtgcgct tattttttat gactggccgc cgattttaca agcagctgcc
     6901 gcattcttca ttgccgcatt tggcgctgca atgatcgttt tattcaaaga tttaagagaa
     6961 cggcctgctg cattgcttca accgaaagca ccgaagccag ggaaacgcat ttttcttgaa
     7021 tacatcactc cgctgtggtc acgccttagc tttaatcaaa aaatcagtta ccgaaatctc
     7081 ttccgatata agtcaagaat gcttatggcg attatcggaa ttgctggatg tgccgggctg
     7141 atggttgcag gcgtgggatt gaaagattct ctaagttctg tctcagctaa acaatttgga
     7201 ccgatcattg actatcaagc aatcgttaca ttggattcat ccacggaaga tagtcgtcaa
     7261 aaagcagcgg atatgctgca gaagcaagaa aaagtaaatg agatcctagc tattcatact
     7321 caaaccgtag aattgagaaa aaaaggacat gcttcgcaaa gtctgacagt gatcgtacca
     7381 agtaaaacag ctgatttgga tcgttttatc catttaactg atcttgaaga gaacccacgc
     7441 tccttgccta aaacaggaat cgctattacg caaaaagcag cagaactatt tgacttatct
     7501 acaggagatc atatttcgct ttatgatgaa gatcaaaaag aatggaaagt cgaagtaaaa
     7561 gaaatcgtga aaaactatct aggtagcttt atttatatgt cccctgaata ttatgcttca
     7621 gttactgatc aatcgatgat ggataatgca tttttgctgc gtacagataa gatgacaaca
     7681 aaagaagaag aaatgctttc cgagaaactg cttgccacag acgctgtgac aaatacgtct
     7741 ttcatgtcca aacaaatcga aacgcaagaa gaatcaatgg cgaacttaga ctcggttgtc
     7801 ttaatctttg tcgtattatc aggactgctg gcttttatcg tcttgtataa tctaactaat
     7861 atcaatattt ccgagcgtgt acgcgaatta tctacaataa aagtccttgg attttttgat
     7921 aaagaagtca cgatgtacat tgttcgagaa aatatcatct ttactctttt aggaatcatc
     7981 ggaggatttg gtgtaggata tatattgact gacttcatct tgcaacaagc gtctatggag
     8041 aatgtcatct ttccattagt gatcacatgg tggtcttatg tcttatcagc aggattaact
     8101 atcgtattta cggttattgt gatgattgtt acccatttca aattaaagca tatcgatatg
     8161 attgatgcgt tgaaatcgaa tgaataaaaa cagaggttgt gaaatcagcg ttttgagcta
     8221 ataacgaggg tcagctgcta attgaacacc gtgaatcaga ggttgtgaaa aagctgtcct
     8281 gcatcgagca aaaggaatac tcaaataaaa ttagctcctc atgttttaca tttgagaaga
     8341 ctgattgccg tagataaaag cttttgagca ccatttatag aagaaacaag aatattttgc
     8401 tattttaaac aagaggatag acaaatacat gtctgatctc ttgtttttta ttgggcaaca
     8461 gacctcttag aatgtataaa gataaatttt acaaacgctt tctgttcagt tataataata
     8521 aatgataact tgtatatata agttgcggta ggtaataaat cagttgaatg gaggtaaata
     8581 tccatgacat ttaaagacaa agtcatttac caaatctatc ccaaatctta caaagacagt
     8641 aatggtgatg gaattggaga tttaaaagga atcaaagaaa aactcccgta tctgaatgat
     8701 ctaggcatcg acatgatttg gctaaaccct atttatccaa gccctcaaaa agacaacggt
     8761 tatgatatct ctgattacac agccatcgat cctatctttg gtacgatgga ggaattcgaa
     8821 cagctaatag ccgaagcaaa gaaataccgg atagaaatca tgctcgatat ggtgctgaat
     8881 cacgtttcga cagaacatga atggttcaaa aaagcactag caggggacca gcattatcag
     8941 gacttcttca ttttaagaga agagccaacg gattgggtat caaaatttgg tggaagtgct
     9001 tggtcgccat ttggagatac ggggaaatat tatttgcact tatatgatcg gacacaagct
     9061 gacttgaact ggcggaacca agcagtaaga gaggaactat tcaaagttgt tcgtttttgg
     9121 atggaaaaag gggtaaaagg attccgtttc gatgttatca atgttatcgg taaagacgaa
     9181 caattgaaaa ataacaaaga aaatgatggc aaacctgaat atacggacaa accaatcacc
     9241 catacgtatc taaaagaatt gaaccaagca acgtttggga cggattccac tatcatcaca
     9301 gtcggtgaaa tgagctctac gacgatcgaa aattgtatct tgtatacaga acctagtaga
     9361 gaagaattat caatggtctt taatttccat catttgaaag tcgattatga aaatgggaat
     9421 aaatggtcta ctcctccatt tgattttgca gaattaaagc gactatttca tacatgggga
     9481 gaaaagatga gtgagggtaa cggctggaat gctctatttt ttaataatca tgatcagcca
     9541 cgtgcgctca atcgatttgt agctatcgat cagtatcgaa aacaaggagc agagatgcta
     9601 gcaaccatga tccatttgaa ccgtgggacg ccctatattt atatgggaga agaaatcggc
     9661 atgatcgacc cagactattc atcgatttcc gactatattg acgtagaaag tttgaatgct
     9721 tataagttac tccttgaaca aggattcaca gaaaaagaag cgtttcgtcg gatcaaagca
     9781 aaatctcgcg ataactctcg aacacccatg caatggtcag acacagaaca agcaggattt
     9841 actacaggga aaccttggtt aaaagttgga ggcaagctag aagagattaa tgtagaaaaa
     9901 gaactatctt ctgaagattc tattttgtcc tattacaaga aattgattcg gctgagaaaa
     9961 acatatccaa tcgttgcaca aggagattat catgcttatg aagcaaatca tccggaagtt
    10021 tatggctttc ttcgccaatt tgaaggacag cagctgcttg tattaacaaa tttctatgat
    10081 aaagatacaa ggatcaccat cccagaagga ttcacagaag ccaatattct tatcgataat
    10141 tattccgata ctgttcttca tcaaagtctg gagctaaaac cataccaggc aattgctttg
    10201 ctgaaaaatt aaaacaaaaa cacaaaacgt cgttcctacg agctattctc gtaagatcga
    10261 cgttttgtgt tttatttaaa ataatcttct tcagaaaatg aagagttttc atactctcct
    10321 ttatcgccat aaaatttatt gtagcgttgt ttgaatttac ggaaaataaa agtaacgagt
    10381 aatttgacta acgtatagat aggaatacca aaaataacgc ccacaattcc cattagatca
    10441 cccatgacaa gtaaaacgat taagatcgta atcggatgga tctgtaaacg atcacccatt
    10501 actcgaggaa tcaccaagtc accgtggaga agttgtacgg caaaccagac aatgacaaat
    10561 ttgacagcca taaaagtaga gtcttggaat gcaatgatca aacctggaaa gaaggcgata
    10621 aacggcccaa tatatggaat gatacataac aacccagcag aaatagctaa aacactagca
    10681 aattctaatc cgatcagtaa gaaacaaacc cagtagactg ctccaaggat aaatgaagca
    10741 atgatttgcc cctttagaaa tgaacccaat tgttgattag caatctttgt caaattttta
    10801 aaatctgtac gaaatgctgg tgggacaatc gctaaaactg actgataaaa tttttgagga
    10861 tctctcaaaa gaaaaaaagc gacaattgga ccagtaaata aagtaataaa aatagctgta
    10921 acagttgaaa aaatgttgct gatccgttgt gcgccacttt cccaataatt tccaataaat
    10981 ccagctatat cagacgtcac gtcgtcaata gaagcgaaaa attgattaaa tgaatcggca
    11041 aaaggtgttt tctctaaaaa ttgtcggaga ctctcttgaa aatcttgaaa caaatctggc
    11101 aaactactga tcaatgtctt tgcctgttct tcaagaaaag gaacaagcca tacgcttcca
    11161 agccccaata aaaataaaat aatcacataa acgataatga ctgacaagtt cctcgaaagg
    11221 tgcttgctca aacgtttgat gactggttcc aaaagataat acaagatcag tgcaaacaat
    11281 aatgggggca agatagtagc gataatgatt ccgacaggct tgaatataaa cgagatctga
    11341 tggaaaaaat aaatgatcag cgcaagtaag ataactattc ctaaaacata gtaagaggtt
    11401 tttcctccta aaaagcggat caaacgattc gttgtatctg tttctttttt cactcttttt
    11461 cctcctttat ctataacttc attatagaca aaagcgggta taaaaggaaa atgatagagg
    11521 ttgattgcaa aaatatcgtc aatcattcat ataaactttc tcatcaatta aaataagaaa
    11581 acgcttgaca gttcttttaa ccatatgata aattaaatcc tgttgctaaa acgtaactat
    11641 tacgttttgt aaaaggcgaa tcggaacatt caaatgaaag aagatgagaa gaggagtgtc
    11701 ataaatgaaa cgattgatag gtatattggc catgctggtt ttagcaggta tgttaactgc
    11761 ttgcggcggg gcaagtggga aagccgagga cagtaaagaa aaattgagcg tgatgacgac
    11821 cttttatcct atgtatgatt ttacaaaagc aatcgtcggg gatgaaggag aagtagagtt
    11881 attgattcca gcagggactg attcccacga ttatgaacct tctgctaaag acatggcaaa
    11941 gatccaagat acagatatct ttgtttataa tgatgaaaac atggaaacat gggtccccgc
    12001 aatccaaaaa acattacaag aaggcaatgt ccatacgata aaagcgacag aaggtatgct
    12061 tttacttccg ggaagtgaag aaggacatga tcatgaccat gaacacgggg aagaaggcca
    12121 tacacatgag ctagacccac atgtatggct agctccaagt ttagcaatca agcaggtagc
    12181 aaacatacgt gatcagctga ttgaagctta cccagaaaaa caggaagtat ggacaaaaaa
    12241 tgcagcagcc tataccgaga aacttcaagc actccatcag ctttatcaag aaacctttaa
    12301 gcaagcaaaa caaagatctt ttgtcacgca acatgctgca ttcaactatc tggcattaga
    12361 atatggattg aatcaagtat cgattgctgg tctttcttct agtgaagaac cgtctgcggc
    12421 gagaattgct gaactgaaac actttgttaa agaacatgga atcaactata tttattttga
    12481 agaaaacgcc aaagatagta ttgccagaac gttagcaaac taagcaggtg tatctttaga
    12541 agtattgaat cctttggaag gattgacgaa tgaacaaata gaaaatggag aaaactatct
    12601 ttctatcatg gaagcaaacc tcgaagcgtt gaaaaaaacg acagagacag aaaacccgct
    12661 agaagatact ttgaccccca aaaaagaaaa gagcgtctac aacggttatt ttgaagacga
    12721 ggatgtacaa gatcgctctc tttcagattg ggcaggaact tggcaaacag tagatacgta
    12781 tgtagaagat gggacttttg atcctgtttt tgaatataaa gaaaaattga atcaggataa
    12841 gacagcacaa gaatataaag aatattatac gaagggctat caaacagata ttgcttcaat
    12901 caagatcagt gagaaagaga tggcatttac gtttaaggat ggaacaacga aacgttcaaa
    12961 ttatcgttat gtagggaaag aaatcttgac ctacgaggca ggaaaccgtg gcgtaaggta
    13021 tctattcgaa gcagaagata aggaaagtgg cgcttttaga tatattcaat tccgtgatca
    13081 cacgattgca ccggctgact ccgaacattt tcatttatat gcaggaaatg aaagtcaaga
    13141 agcattgctg aaagaaatgg agaattggcc aacattttat ccagatacaa tggatggacg
    13201 tgaaattgca caggaaatgc ttttgcactg ataggcaaaa aagaaccgaa gggtttggat
    13261 cggctagtca tttagtcggt ccaaactatt cggttctttt tatgaaatca atttctttaa
    13321 tccacggata gaggctggga tggtctcgcc gtttgccaac gtgacttctc tggttttttt
    13381 attaatcgac cgcacatttt gtgtattgat aacataggat ttatgacaac ggataaaatt
    13441 ccgatgcatt ttctcgattt ctttgatctt ctcatagaat tgtaattggg catgagtcaa
    13501 atggacttcc agtttatgtg gtgctcctag cgaagtagaa aaatattgga tgtctttgat
    13561 cggtaaaaat ttgatctcat cattaaactc tattttgaag atatcttttt ggatctggga
    13621 catttgaaaa tcttcgaaaa ttttgctcag gatatgctcg acattttctt ttaaatcata
    13681 aagatcttct ttgacgatat aatcagtcgg ttcgatgttg ctttttaaga ttttcatcga
    13741 catttccgta tgggaagtca catagattat ttttcctaag ggatctaatt cacggatcaa
    13801 tttaccgatc gctacaccat ccatttcact gtcttctagt tcgatatcta aaaagtaaat
    13861 accaaaagaa gaagttctga tcttggctgc attgagaagt tcttccggat cagaggtaga
    13921 aatatggaca taactgtcta agttttcaat catgattcta tttttgatca ccgttgataa
    13981 aacttctaat tgttttggat cgtcttcaca aatataaatg ggaatcatat cattactcct
    14041 ttattttatt tgtatataaa tcgtttgaga aaaacgctgg ttttcaattg atgtttcaag
    14101 gaaaagataa ggattggtct ttgtcaattc atccaagata aacaagccaa ggccttcgtt
    14161 ttttggattt gtttttgttg agaacccttt ttgtttcaac tggtttaacg gcaatgtatt
    14221 tgttgttgta ttgatcacac gaatcatcac ggaatgattc ttcttgagta tggtcaattc
    14281 gatttttttc ttctttgcag cactagctcc ttcaatcgca ttgtccaaga taataccaag
    14341 catacgtaca aggtttaccg tatgtttcgt atccagttcg attggctcag gaaaatgaag
    14401 ctggacgacc aacccttttt cctgtgccat catgagtttt aatgacagta aactacggat
    14461 ctcaggaact ttcatattat ccaacatact tagattggcc ggaaaaaggt tcatcatctg
    14521 gcgtgtaggt aatatcgttt gagcataata agaccgtaat ccttctaagt cgccttctga
    14581 aatatagctg tgaagagaaa aaagcagatt ttggtaatca tgtttgaatt ctctcaagtc
    14641 ttgatagttt ttttctagat ctttgatata atcctgttgc atcctgactt gttgctctaa
    14701 taactgatca gctaatttct tattagagat tttccaataa taaaccaaga tgatcaggcc
    14761 aacacacaga tatagaatga aaaaactcgt attcaaagct aagacatcag gtgtttcacc
    14821 agaaaaacgg gtataaaaaa taatgatata gtatgtaaat aatatcaatg tcccgatcgt
    14881 accgatcata cggcggtcca cagtgtgaaa ccaagtctct aacgattttt taaaacaaaa
    14941 agctaataaa gcagataaaa aagcggataa ggcaatgtga aaatactgca atgaatctcc
    15001 tggttcaata accgaagatt tcaaaacaaa aaaatcgccg atgcttgtta agtgatctcc
    15061 taaaagcgac gtcaataacc caaaaggaag cgcgtaaacc aattgttgac gcgaagtcga
    15121 aaagaagaaa aaagtaagca tcagcattcc tatagcaatt agagcagtaa aaatccctaa
    15181 aatcgaatag acccatccaa tcaaaataca agaaatcaga atgactcccg caaacagata
    15241 tttgtatcgg atgtgatggt caatcaataa gatacaaatt gtgtaaatca aatattgcaa
    15301 cgctatggac cataaaaaca taaagtacag ctcccattga tttttaccaa attatagcac
    15361 aattagcgta ttattttttt aatttttcag ggattttgaa ttcagaactc ataccaaagc
    15421 agcagccaga tacaaaaaag tcagataagt tagttaattg tttaatgatt gtttttttca
    15481 tgaaagattc ctccaaaatt catcaagtag tttgtaatag gaagcaacgt agctatttct
    15541 aaaacaatcc caagtagcaa ataggtagaa aagtaagaag ctgagaaaaa taaagtaatg
    15601 agagaaaaaa ttagaaacat gctgatcgcc ttctttaata atgggggacg tttctcgggt
    15661 tcaatgggat tgattgctgt acctcttgat ccatagcgaa gcagtaacaa aaagcagctg
    15721 attaacaaaa agaccatcat tgggaaggag atagtcaatt gcagatgttt catcaccaag
    15781 ggaacgcccc ataaataaag tagtgtccac agcagacaaa cgaattcaga gtcggaatgc
    15841 aatccataag catatctgcg gatgcatata taagaaagaa aggtgattcc tgtttccgcc
    15901 catgagcctg tcaccagaga aaagactaga acgaccgcta gttttccaag attattcaat
    15961 aaaacttcta aggcaaattt tacttggaca tacaccatat cttcttccga tttgtctccg
    16021 ctgaataagg cttctaataa tttgttcaca actttatctt tcatctcata cgtacctcta
    16081 ccacaaattt tatgctaaaa aaaataaaaa acgtcttttg tttcataaaa agtggttttg
    16141 acatcaaaac cgtcatgatt tcctaaaaaa atcttcgttt tccaagaacc ttaccaaaaa
    16201 aagtgacagt tttgatacga aaagtacttt ttatgacaag tttgtgcttt ttcgagtgat
    16261 ttatgttaaa aaaattactg accttataaa aggtgatgga gggattctta tggaaaatga
    16321 acaattgttt gaagtcggcg aaacatatgc atttaaatct gatttatttg aatcaccaat
    16381 aaaaggaaaa atagaaaaaa tctatgaaaa tagtgtgatg atacgtgtca tgaaatgcgc
    16441 agtagaagac aatgataccg ctcataatct gcactggcag ttagttgtac gcaaatcaag
    16501 tacaatagaa gaataaaaac agataaaatg ttgagagcat aaagaggttg tgagtctccg
    16561 tttagctctg agttacaagg aataatgtta aaaaacagct tttcatgttt tatgaatatt
    16621 gccacttgtg accgaggtgt tggcaggaga atactgttta tttgaaaaaa gggctgcgac
    16681 aagacttttg tcacagcctt tattttcatg aataggagtt acttttggct acttttagag
    16741 aagtagcttt taaggagcag ataaagtcaa ggcttttatt tctataaatg aagtagtaaa
    16801 atgaagaaca gattttatta caggaaggat gccgataatt gtgatcattg ttgcaggaac
    16861 gatcggtgca gggaaaagca cactgactga aatgcttgcc caagatttag aaacaaaacc
    16921 tttttatgag aacgtggaag ataatgaagt attgccgcta ttttattcta atccagaaaa
    16981 gtatactttt ttattacaga ttttcttttt gaacaaacgc tttttggcaa tcaaagatgc
    17041 tttcagccat gatgacaatg tactagaccg ctccatctac gaagatagta tgctgtttca
    17101 tttgaatgca gatttagggc gtgtttctga agtagaagta aagcagtatg aaggtctttt
    17161 agaaacgatg ctgaaggagt tagaagaaat ttcgccacaa aagaaaccag atctactggt
    17221 ttatattcgt gtatcttttg aaactatgtt agcgagaatc aaaaaaagag gccgggaata
    17281 tgaacagcta gaacaagatc ctgaactcta ttcctactat aaagaactca atcgacgata
    17341 cgaagagtgg tatgaacagt ttgatatatg tcccaaaata gtgatcgatg gggacaaata
    17401 tgatttcgta gccgatccgg catgtggaga acaaatcgta caagaaatca agatgcgggc
    17461 aaagaaaatg acagaagagg cagatgccca gtcgatccgc tacactacaa ataacccgaa
    17521 agcaaaggga atctttacaa ctaaataata taaaaactaa gcaaattgct cactcgcaga
    17581 tatttctctg tgagcagcat cttgcttagt ttttttgatt cattgagatc aaatagtgaa
    17641 ccgattcgta tgataaaatt gccataaatg aaatgtctga gtgagggcca tgatcaaaaa
    17701 taaaatggaa acgatcaaaa tcgaatgggc gccaaacaga tgatgacaga aaacaaggac
    17761 aatagctcct gcgataaagg caagaacgac acttagatag tggaaaaatc gatcaaaatc
    17821 tgctttttct ttagaaatac aataatcata taagttcgtt gctacccctt ttaaatttcc
    17881 agttgtaaat aaattagtgt aggcacgccc gttgatttta tcaaaagcta cccactggat
    17941 cgatgcagta aaagcaatca aaatcgttac taatgcggga tgctcatgga cgggtgtcaa
    18001 actaacaaag aaaaaaatca ctgcttcata atatagaaca aacaagcgcc agaaatgttt
    18061 ttcttttttt ctaaaataat caattagata ctttgttaga agaatcccta agaaaaagaa
    18121 aagggtagac aatacttttt taccaacact tagccattct ccgtcaaatg cttggatgat
    18181 cgatagaatg atattgccag tttgagcaga ggcaaaagca ccatattgga tataagtata
    18241 actgtccatg gctcctccga taaatgtcag caaaagtcct attgtacgcc cctcgtgaaa
    18301 ggaagtctgc atttttttca tcacctttta tgaatatttt tgtcttcctt atcttagcat
    18361 aaacgattga aaaaaaggaa aaattttcag aaaactaaaa tcatatcgct tcccattttc
    18421 atttttagaa aggtctgtta gtataaagaa aaaatagaca acgaaacttt aaataaccaa
    18481 cttgaaataa ccaacttgaa ataaacaaat tcaaagaaga aagatgggaa ggaaatcaat
    18541 cagttgaatg aaaaagaaat tctccgatta caaaaagaat tgaaacgctg ggagaacaag
    18601 tataaaaagc ttcaacaagt ctttcggcta caagaagaaa tcattgctac ctatgaagcg
    18661 gcttatggcg aagcacccgg acttattgag gaagaagtac aagtaaaaga aagtgaagga
    18721 aacagctctt tctttaaaat gatggagaaa cgttcctata atcgcttcaa tcaagaacaa
    18781 aaagcagcta tcacatatga tatgaagaaa catcttcgaa tcatagcagg agctgggagc
    18841 ggcaaaacgc aaacaatctg tgcaaaagct gtttatctga tgacgcaaaa acaagtagac
    18901 gaagaacgca tactaatgat cacttttaca aggaacgctg caaatgaact aaaaaaacga
    18961 gtagataatt tcagccagag aaaaaccgct gtccatatcg gaacattcca tagtatcttt
    19021 tttaggatat acaatgaaat ttgtcgaaaa tttcctgaag tagcaatgca aggaatacaa
    19081 ggagactttt caaaggatac tgcacaaaaa gtgaatgctg ttttgcaaca gctgatacgt
    19141 aaatataatc tttatatatt tgatcaatat ggagaaaaaa cgattgcttc tcggttagac
    19201 tattggcaga atatgaacct ttcagtagaa gaaatggttc agttagttaa agaaaaatat
    19261 gattccattg ataagaatgc acgacagccg atatcagaac gactgtatgg attgctcacg
    19321 gaactccaag aacaaaaaag aagccagcaa ttgctagagt tcaatgatgt actccaaaat
    19381 ctgaaaagcg ctttggagaa tgaggagatc cagcattata tcggccaaaa atatgattat
    19441 ctattcattg atgaatttca agatactaat ccacttcaat gggaaatcgt gaaacggatg
    19501 acaaaggaaa acaggatcaa gttgattgtt gttggagatg atgatcagag tatctatgga
    19561 ttcagaggat cagagccagc atacatcaag aactttgaaa aagaatatcc taccaaaact
    19621 cttttcttat taactaatta tcgttctcgg gcagctattg tccaaggagc aaatagattg
    19681 atcagctata ataaaaatga tcggatacca aaggcgatga ttccagcaca aaaagaagca
    19741 ggtgttatgg aagcctattt gttttctgat actaaagcag aaagtcagtg gctaatcaaa
    19801 caaattcaga catttattca aaaaaatggg aaatataagg aatcaatcat attataccga
    19861 tcagcttcac aaacgcagca gcttgtccaa tctctgctga aaacgaatat tccttttgta
    19921 ctggaagcag atagtccata tgaaggtatc tttggtatca agtctttcca acatttctat
    19981 caaaaaattg ttcattggca aacagcactg aatctgcaaa agaaaaatca ggcctaccag
    20041 caattgctcc ggcagctgat ggcagactgc tacctgaaaa aaagtgaatg cgatcaatac
    20101 ttttctccta aatatcaaaa tatagccgtt gcagattata ttttgtcgat aagccccaat
    20161 ttaaaatcaa aatctgcaga aatccgagag tttgaggaag cgttgcgtaa aacagaccag
    20221 catcaagaaa tctattcgct tgttcaaagc tatcttcatc tgccacgtat agctaaggaa
    20281 atcgaccaga gtgatcaaga atggatacga gaagaagtaa agcagcatgc tactttcaga
    20341 agactccaag ccttatcgca agaaacccaa gcgacagcta aagaattaaa aacccgcttg
    20401 attgcttaca gacaaggaaa acttgatgca ctatgtatcc agagcattca caaaagtaaa
    20461 ggtcttagtt accgaaatgt ctttttgatt ggttgtaacg aaggaagttt gccttataac
    20521 ggagcgacag aaaagaaagt aatcgacttt aatgaaataa aagcagaacc agtgacgacg
    20581 atagaagaag aacgaagact attttatgtt gcgatgacta gagcaagaaa tcgtctgtat
    20641 ctatgtgtac ctcaaatgaa aaattcaaga aaattgaaag tatcgagatt tatcaaagaa
    20701 accgggagtc gtgtgaaaaa aggaaagtga tagaagagag ggtgtgacag aagtggtcag
    20761 tttcaagaaa gaagaaggaa tgtacgaaaa ttgcttctca aatttttgtg aaatttcagc
    20821 ttattttcga agaagctatt tctggaacac cgtttattcg gaaaaaggag gtgtgacaag
    20881 aattttgtca cacctccctt atttattttg aa
//
LOCUS       DAKBVG010000025        20264 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000025 DAKBVG010000000
VERSION     DAKBVG010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 20264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.025"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(61..1620)
                     /locus_tag="OUD09_000709"
     CDS             complement(61..1620)
                     /locus_tag="OUD09_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HCT9692003.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            1775..4009
                     /gene="pcrA"
                     /locus_tag="OUD09_000710"
     CDS             1775..4009
                     /gene="pcrA"
                     /locus_tag="OUD09_000710"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HCT9692004.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            4026..6062
                     /gene="ligA"
                     /locus_tag="OUD09_000711"
     CDS             4026..6062
                     /gene="ligA"
                     /locus_tag="OUD09_000711"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCT9692005.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            6168..6473
                     /gene="gatC"
                     /locus_tag="OUD09_000712"
     CDS             6168..6473
                     /gene="gatC"
                     /locus_tag="OUD09_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HCT9692006.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            6473..7945
                     /gene="gatA"
                     /locus_tag="OUD09_000713"
     CDS             6473..7945
                     /gene="gatA"
                     /locus_tag="OUD09_000713"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HCT9692007.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            7942..9372
                     /gene="gatB"
                     /locus_tag="OUD09_000714"
     CDS             7942..9372
                     /gene="gatB"
                     /locus_tag="OUD09_000714"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HCT9692008.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            9382..10437
                     /locus_tag="OUD09_000715"
     CDS             9382..10437
                     /locus_tag="OUD09_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCT9692009.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            10548..11921
                     /gene="rlmD"
                     /locus_tag="OUD09_000716"
     CDS             10548..11921
                     /gene="rlmD"
                     /locus_tag="OUD09_000716"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCT9692010.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            11924..12052
                     /locus_tag="OUD09_000717"
     CDS             11924..12052
                     /locus_tag="OUD09_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCT9692011.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            12492..12680
                     /locus_tag="OUD09_000718"
     CDS             12492..12680
                     /locus_tag="OUD09_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HCT9692012.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            12758..13771
                     /locus_tag="OUD09_000719"
     CDS             12758..13771
                     /locus_tag="OUD09_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCT9692013.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(13838..14872)
                     /locus_tag="OUD09_000720"
     CDS             complement(13838..14872)
                     /locus_tag="OUD09_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCT9692014.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            14987..17212
                     /locus_tag="OUD09_000721"
     CDS             14987..17212
                     /locus_tag="OUD09_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCT9692015.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            17230..19113
                     /locus_tag="OUD09_000722"
     CDS             17230..19113
                     /locus_tag="OUD09_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HCT9692016.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDIK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            19094..20068
                     /locus_tag="OUD09_000723"
     CDS             19094..20068
                     /locus_tag="OUD09_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HCT9692017.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
BASE COUNT         6836 a         3713 c         4342 g         5373 t
ORIGIN      
        1 atcatataga atcaagtagt gtagatgaat agttgttgta aaatagtttt tccatgttcg
       61 ttaaaatgat tgcttcaaat ttgtgataga agatttagcc tcgatttcct gcttgattct
      121 tggcaagttt gcttcatcag tcaagataac caaaatatct cctacattca tgatcgtatc
      181 accgtgagtc aagatttcct gatttcctct gcgtatagag attagcagca tatttttagg
      241 ccaattgaag tctcgtacca ttaatccgtc taaagaactc tctgccgtta ctggaaaatc
      301 aaaagttgtc cttgcacctt ttagtgaggc ggtctgagta tttccaagta gacgttccaa
      361 taatgattcg taaattggat taccgccgag aaggtcattg acgatataag caacaagagc
      421 agccaatcct aaaggcatca gatgattaag agagccaacc atttctgtca ctaaaataat
      481 tgctgtcagc ggtgcttttc caatcgctgt gaaataacca gccatggcaa taacgacaaa
      541 atccttgatg tagattggat ccatcccgaa atcaaccata atgtttccat aaaagctacc
      601 gattaaagcc cccaatgtca atatcggaag gaaaatcccc cctggaagac ccccaccgta
      661 agaaatcatg gaaaaaacga atcgaagaac aaacaaaccg ataatcataa tgattggcaa
      721 ggaagcatgt ccaaaagcta aaacgatttg attgcctccg cctagagtac taggccataa
      781 ataaccaatt ggcaaaatca gcaagaaagg aacgaacccg tgaaaataag ctggtaaagg
      841 caatcggttg taaaattttg gcaaagccag caagacattc tgataaataa aacctaagat
      901 gcctagaagt actcctaaga tcactaagga accataatat ttcaacggca aagaaggaag
      961 gtcacctaga tataaaacag gtctcaagcc gaaaaagtaa agagagacga agttagcagt
     1021 gattgctgaa gcaaatgatg tcagccaaac aagtggagaa aaggagtgat ggatttcttc
     1081 taaaacaaag agtaatcctg cgataggagc attgaatgct gctgcaagtc cgctacttgc
     1141 accgctagaa attaaaatct tttcttctga agatggactc cgaaacaatt gtccagctcc
     1201 ttgtccaatg gatgcgccaa gctgtataga aggtccttct ctacctagaa aaaggcccgc
     1261 accaactgaa agtacaccgc caatgaactt tttccataaa acagagaacc agttcattga
     1321 taattccccg cgaagctgtc cctctacttg tggaatcccg cttcccttga tatccggttc
     1381 atttttgacc agataaccca cgaaaaatgc tgcaaggatg cagcaaatag ctaggccaat
     1441 taagtaaatc ggctgttccc gactcatttc atagatactt cgaactaaat cgcttagctt
     1501 ttcgatagat aatcgaaaca ggctaacgac gattcctgtc agcagtccaa caaatattcc
     1561 ttttagaata aaatatatct gagtcttatc taatttcttg atttttcctt ttttttccat
     1621 tcaaacgcct cgctttcttt taatttagcg cgaaatcaac gaattgacaa ggaaaaatcc
     1681 ataaaaagaa acaaatgatt ttgaactagc tagtttaatg aaagccaaga aaatgctata
     1741 attaacaaga ttgataaaag aaaggatgtt gttaatgtca tccaaagctg aattattaaa
     1801 cgggatgaac ccccgtcaga aagaagctgt tcttcacact gacggaccat tactgctaat
     1861 ggcaggtgca ggaagtggaa agacgagagt gctgacacac cgcattgcct atttgattga
     1921 ggaaaaagaa gtcaatcctt ggaacatcct agcgattact tttaccaata aggctgcaaa
     1981 agaaatgaaa gaacgtgtca atgcgattct cgcatctgga ggcgaagatg tctgggtatc
     2041 gacttttcac tcgatgtgtg tgcgtatttt aagaagagat gttgatttta tcgggtataa
     2101 ccggaacttt acaatcatcg attcttctga acagctgacc ttgatgaaac ggatactaaa
     2161 agaactgaac attgatccga aaaaatatga tcctcgaagt atattaggaa caatctctca
     2221 agcaaaaaac agcctgcaga cccctcagga ttttgcaaaa atgcaaggca gttattacga
     2281 agagattgcg gcaaaatgtt atgctgctta ccaaaaagag cttcaatata atcaatgtat
     2341 ggactttgac gacctgatca tgaatacgat tcgattattt gaagaacatc ctgattcact
     2401 cacgtattat caaaacaaat tccattatat tcatgtagat gagtaccaag atacaaatca
     2461 cgcacagtat acgctagtca atttattggc aggtagattt aggaacctgt gcgtcgtggg
     2521 agatgcagat caaagtattt acggctggcg cggtgcagat atgcagaaca tcttggattt
     2581 tgaaaaagat tatccagatg ctgcagtaat tttgctagaa caaaactatc gctccacgaa
     2641 aaacatcctt agcgcagcaa accaagtcat tgaaaacaat agcaatcgaa aaccaaaaaa
     2701 cttgtggaca gaaaacaaag aaggaaacaa gatcacttac taccgtgcag ataatgaacg
     2761 agacgaaacc cgttttatcg tagaccggat gcaagaagag attcgaagca atcatcggaa
     2821 ttacggcgac tttgccatct tgtaccggac gaacgcacaa tctcgtgtaa tggaagaaac
     2881 tctgttgaaa gcaaatattc cttataaaat ggttggaggg cacaagttct acgatcgtaa
     2941 agaaatcaaa gatattttag cgtatttgaa tgtgctggta aatcctcaag attcaatcag
     3001 ttttgagcgt atcgttaatt cgcccaaacg aggcatcgga ccaggatcta tcgaaaaact
     3061 acgcagtttt gcttctcttc atgaatggcc actgcttgaa gcagcgcaaa atgtggatct
     3121 ggcaaatatc ggtggaaaag caggacagca attaggtgca tttggagaga tgatccaaga
     3181 agtcactcag atgatcccat acttgacagt gacagaattg acgaaagagg ttttggatcg
     3241 tagcggatat ctagaagact taaaaatcca aaatacatta gaagcacaag cacgcatcga
     3301 aaacttggag gaatttttaa cggtcacaca agaattcgac aaacaattcg aacagcaaaa
     3361 tgaagaagac gctgatgcac cagaagaaaa actaacggtc ttcttaaatg atctggcact
     3421 tgtttccgac attgacaatt tagaagaaga tgcttcccaa gtgacattga tgactttgca
     3481 tgcagccaaa ggattagaat ttccagttgt attcctgatt ggtctagaag agggggtttt
     3541 tcctttatct cgagcattga tggaagaaag cgaactggag gaagaacgcc gtctcgcata
     3601 tgtggggatc acgcgagcag aagaagcgtt gtatttaaca aatgctttct ccagaacatt
     3661 atatggacgg acgcaatata accgaccttc tcgttttgta gaagaaatcg atcaggagtt
     3721 actggaaatc gaaggcatgc gtcctacgcc taaaaaaacg cctgtattcg ctaaaaaaac
     3781 agcatatagc tataaacagc cagagacagc ggttgttccc tctaaaagtg ctactggagg
     3841 ggaaaataac agctggaaac ctggagataa agtcaaacac aaaaaatggg gtctaggaac
     3901 agtcgtccga gtaagcggga cgtcgaagga tctagaacta gatgtggctt tcccaagtca
     3961 aggagtaaaa cgtcttttgg ctgcctttgc gccaattgaa aaagcttagt agtggagggt
     4021 aaatgatgga acatgaaatg acgattgacg aagctgctaa acgagcagaa gaacttcgta
     4081 caagactgaa ccaatggtct cgcgaatact acgtagaaga taaaccaaca gtggaagact
     4141 atgtttatga taaagaatac gcagaattag tagcaatcga ggaacaatat cctgatttga
     4201 tcacgtctga ttctcccact caacgagtag gcggaaaagt attagaaggt tttgaaaaag
     4261 tcactcatga tattccgctt tacagtttga acgacgtttt cagcaaagaa gagctgatag
     4321 cttttgatca acgtgtacaa aaagcagtgg gacgtgtagt tgactattgc tgtgaactaa
     4381 aaatagatgg actttccgtt tctttgcgtt atgaagatgg aaatttcgtg agaggtgcga
     4441 ctcgcgggga tggaactgta ggagaaaaca tcactgaaaa cctaaaaact gtccggtcgg
     4501 tcccaatcaa attaaaagaa ccaatgaaca tcgaagtaag aggcgaatgt ttcatgccaa
     4561 aacggtcatt tgtccaattg aaccaagaca gagaagcaga gggaaaagat atctttgcga
     4621 atcctcggaa cgccgcagct ggttctttgc gtcagttaga ttctaaaatc acagcgaaaa
     4681 gaaatctgga cactttttta tacacagtag ctgatttcgg tcctatgcaa gcaaaaaccc
     4741 agtatgatgc gctagaagag ctagaaaaaa ttggattcca cacgaatcgg gaaaaacgtc
     4801 tatgtcattc gattgatgaa gtgtggtcat acattgaaga atatcatgac aaacgtgtgg
     4861 atttgcccta tgaaatcgat ggaatcgtca tcaaagtgaa tgaattctcg ctacaagatc
     4921 aacttggttt tactatcaaa gcaccgagat gggcagcagc ttacaagttt ccgccagaag
     4981 aagttgaaac gctaatcgaa aacatcgaat ggacagtcgg tcgaacaggg gtagtaacgc
     5041 caacagctat catgacacca gtccgagtag caggaacgac agtaagccgt gccagcctgc
     5101 ataacggtga ttatattaag ttgaaggata tccgcttgaa agatacagtt ctgatttata
     5161 aagcgggaga catcatccca gaagtttcgc aagtcgtgtt agataaacga cctaaagaca
     5221 gcgaagaata ccaattgcct acacattgtc cagtttgtgg aagtgaactt gtccatttgg
     5281 atgaagaagt agccttacgg tgcatcaatc ctaaatgccc tgctcaaatg aaagaaggtt
     5341 tgaaccactt tgtttcaaga aatgcgatga atatcgatgg gttggggccg cgcgtactag
     5401 aacagatgta tgataaaaaa ctagtagcag atgttgctga tctttacaaa ttgacagaag
     5461 aagagttatt gacgttggac aaaataaaag aaaaatcagc aaataatatt ttgacagcaa
     5521 tcgacaacag caaagataat tcggtcgagc ggttgatttt cgggttagga atccgacatg
     5581 tcggagcgaa agcagcaaaa attttagcag aacatttcgg tgatctagaa acattgagta
     5641 aaagtgacta tgagtcgatt attgcgcttg atacgatcgg tgatatcatt gccgacagtg
     5701 tagtcactta tttcagtaac gaagaagtcc atgaattgat gaatgaatta aaacaagcag
     5761 gcgtcaattt cgaatacaaa gggcttcgaa atgcccaatt gcaagaagtc gagtctcctt
     5821 ttaaagaaaa aacagtagtc cttactggta aactaacacg atttactcgt gaagaagcaa
     5881 aagagacgat cgaaaatctt ggcgggaaag tgactggtag cgtatcgaaa aaaacggata
     5941 tcgtggtagc aggagaagat gctggcagca agctgaccaa agcacaagaa ttaggaatcg
     6001 aagtttggac agaagatcaa atggcagatg cattggctaa aagtcgctca gtagaagaat
     6061 gaaaaatgag ttttttcaga caaatttcaa taaacaattt cagacacggc actcttgtgt
     6121 taaaatagag agcaaagttt ttaactcgtt tagaaagaag gggtagtatg gcgattagtg
     6181 aagaacaagt gaaacacgtt gccaaattag caaaacttgc gttcgcagat gaagaacttg
     6241 caagttttac cgaccaatta ggtaaaatca tcgatatggt tgaaatgctg gaagaagtag
     6301 atacggaagg tgttccattt acgtcgaatg tggttgaaac agtaaacgtg atgcgacaag
     6361 atcgggcgca agctggatgg aaccgcgatg aattattgaa aaatgtgccg gaaacagaag
     6421 acggctttat caaagtaccc gcaatcatcg ataatgggga ggctggcgca taatgacaga
     6481 attacacaat cgttctctag aagaactgca tcatatgctt gtatcgaaag aaatcactgt
     6541 acaagatttg acaaaaacaa catttgaacg aatcaaagaa acagagcctg aggtcgaggc
     6601 attcattaca ttgaacgaag aaaaagcatt ggaacaggca aaagctttag atgaaaaagg
     6661 gattgatgaa agcaacgtcc ttgctggtat tccaattggt atcaaggata atatcgtaac
     6721 aaaagatatc ttgacaaccg ctgcatctaa gatcctttac aattttgaac caatttatga
     6781 tgcaacggtc atggataaaa tctatcaagc agatatgatc cctgttggta agctaaacat
     6841 ggatgaattt gctatgggtg gaagtacaga aacttcttat ttcaagaaaa caaaaaatgc
     6901 ttgggaccca acaaaagtac ctggtggctc ttctggtggt tctgcagcag cagttgcagc
     6961 tggacaaatt ccagtttctt taggtagtga tactggggga agtatccgcc aaccagcagc
     7021 ttttaacggc atcgtaggga tgaaacctac ttatggccgt gtctcacgtt ttggtttgat
     7081 tgcctttgct tcatctcttg accaaatcgg accaatgacc cgtacggtga aagataatgc
     7141 attagcattg acagctatca gcggttatga cgaaaaagac ggtacttctt caggcgtttc
     7201 agttcctgat tttgcagagg gactgacagg cgacatcaaa ggaatgaaga ttgctttacc
     7261 taaagaatac ttaggcgaag gtgtggcccc tggcgtaaaa gaagctgtat tgaaagcagc
     7321 agaaacattc cgttcattag gtgctgttgt ggaagaagtc agcttgcctc attcaaaata
     7381 cggtgtggct gtttattata tcattgcctc atcagaagca agttccaatt tgcaacgatt
     7441 cgatggtatc cgctacggtt atcgctctga aaatgtgaaa tcattagatg atgtatatgt
     7501 caactcacgt tcagaaggat tcggtgaaga agtaaaacgc cgtatcatgt taggtacatt
     7561 ctccttaagc gcgggttatt atgacgctta cttcaaaaaa gcaggacaag tccgtacatt
     7621 gatcaaacga gattttgaaa aagtctttgc agattatgac ttgatcatcg ggccatcttc
     7681 accaacagtt gcattcggaa tcggtgagaa cattaacgat ccaatcacaa tgtacatgag
     7741 tgacatctta acgatccctg tcaacttggc tggacttcca ggtatgtctg taccggcagg
     7801 tttatcagaa ggattaccag ttggattgca gatcatcggt aattattttg atgaaaaaac
     7861 gatgtatcaa gcggcatatg cgtttgaaca agcaacagaa ttccataaac aacaaccagc
     7921 tattttagga gggaaagacg aatgaatttt gaaacagtca ttggactaga agtccacgtc
     7981 gagttaaaaa ccaactcaaa aattttctct ccagcgccag ctcattttgg tgcagaacca
     8041 aacagcaata caaacgtgat cgactggggt tatccaggcg tgctgccagt aatgaataaa
     8101 gctgcgctgg aattcgggat gaaagcagct ttggcattaa actgtgaaat ctctaaagat
     8161 actcattttg accggaaaaa ttacttttac ccagataatc caaaagcata tcaaatttcc
     8221 caatttgatc aaccaatcgg tcatgacgga tggatcgaaa tcgaagtaga aggtaaaaag
     8281 aaaaaaatcc ggatcgaacg tgtccattta gaagaagatg ctggaaaaaa catgcatggt
     8341 attggcggtt attcttacgt tgatttgaac cgacaaggca cgcctttgat cgaaatcgtt
     8401 tctgaagctg atatgcgttc accagaagaa gcatacgctt atctagaagc attgcgatca
     8461 attatccaat ttacagaagt cagcgatgtg aaaatggaag aaggctctat gcgctgtgat
     8521 gccaatattt ctcttcgccc ttatggacaa gaagagttcg gtacgaaagc agaattgaaa
     8581 aacttgaact ctttatcttt cgtgaaaaaa ggtttagctt ttgaagaaaa acgtcaagca
     8641 aaagttcttc tttcaggagg agaaatccaa caagaaacac gccgttttga cgaaacaacg
     8701 aataaaacag ttttgatgcg tgtcaaagaa gggtctagtg attatcgtta cttcccagag
     8761 cctgatatcc cgaaattcgt gatcgatgac gaatggattc aaaaagtaaa agctagtttg
     8821 cctgagatgc cagcttctcg tcgcgaacgc tatatccgtg agtttggact tccagagtat
     8881 gacgcaatgg tattgacact tacaaaagaa atgtctgatt tttttgaagc tgttttggaa
     8941 aatggtgcag atgccaagca agcatctaac tggttgatgg gtgaagtttc tgcttacttg
     9001 aacagtgaga aactggaatt agctgagaca aaattgacac ctgagaactt agcaggaatg
     9061 attcgtttga tcgccgatgg aacgatcagc tcaaaaatcg ccaaaaaagt cttccgtgaa
     9121 ttgatcacaa atggcggaga agcccaagaa gtagttgaaa aaaatggttg ggtgcaatta
     9181 tctgatccga acaaactact gccaatcatt aatgaaattt tggataacaa ccaacaatct
     9241 gtcgatgatt tcaaaaatgg aaaagatcgt gcggttggct tcctagtcgg tcaaatcatg
     9301 aaagcaacta aaggacaagc aaatcctggt gtcgtgaata agctacttaa agatgaatta
     9361 gcgaaacgat agaggctgaa aatgaaaaaa gcacgcgtaa tttataaccc cacctcggga
     9421 aaagaattat tgaaaaaaaa tttggcggat atcctacagg cattagaaga agcaggattt
     9481 gaagccagcg cctatgcaac tacaccagaa ccagattcag ccaagaatga agcatgtaga
     9541 gtcgcagaac taggttttga tctagtagtg gcagctggtg gcgatggcac aatcaacgaa
     9601 gtggttaatg gaatcgctcc gcttgaacac cgtcctaaaa tggccatcat cccagctggt
     9661 acaacgaacg actatgctcg agcattaaaa attcctagag ataatattaa agcagcagca
     9721 gaagtgataa aaaaacacca aaccgtaaaa atggacatcg gcctttctga taacagttac
     9781 ttcatcaata tcgctgcagg tggctatttg acagaattga cttatgaagt tccatcagaa
     9841 ctaaagagta tttttggtta tcttgcttat cttgcgaaag gagcagagat gctccctcga
     9901 gtaaagccaa tcaaaatgcg cttgaagtat gatgaagggg aatatatcgg aaatgcttcg
     9961 atgtttttcc tagggctgac gaactctgtt ggaggattcg aacagatcgc gccagatgcc
    10021 aaattggatg atgggaagtt ttctttaatc attgtgaaga cagcaaatat ctttgagatc
    10081 ttgcatattg cagcactcat gctgaatggc ggtcgacatg tcgatgatcc aagaataatc
    10141 tataccaaaa caagcagttt gttcgttgaa acagaagatg atatcaatcg accagtaatg
    10201 atcaacttag atggtgaata cggcggggaa gcaccgatgc atttccaaaa tcttcatcaa
    10261 cacattgagt tctttgccaa taccgatcag attcctgatg aagcaatcac aggtacagat
    10321 gaagaagaat tagaggttgt cagcaaagaa ttcgtaaaag aagtcgaacg tttaacggat
    10381 gaagatatcg acggtgacgg aaaaatagca gacgaaacac ctaatcaaaa gaattaatca
    10441 aaagccggtg ctgaacgtag taaggtcagt gccggtttta gttgtgtaat tagctgattt
    10501 atagtagagt tcagtaggat tgaaaaatag gcttaaagga gcaaaatatg acagaacagc
    10561 aaatagtaaa gaaaaacgaa cgatatacag tcgaaatcgt cgacttgagt tatgaaggaa
    10621 tgggggtagc aaaaatcgat ggttatccat tatttatcga aaatgcactc cctggagaag
    10681 aagtagaaat actagtagta aaagcaggca ataagtttgg atatggaaaa gtcgaaacat
    10741 ttattacaca atcacctgat cgtcagcctg tagaaaacaa tgcattactt cgcataggta
    10801 tcgcaccttt agcccatttg aagtatgaac aacagcttgt cttcaaacaa aaacaagtag
    10861 agcatgtaat ggcgaagatt gctaagatgc cagaagttcc tgtacttcca acggtcggaa
    10921 tggcagaacc attcggctac cgtaataaag cacagatccc tgttcgccgt atcgatggac
    10981 agttagccac cggtttttac aaaaagaata gccatgatct agtccctatc gaagattttt
    11041 atatccaaga tcctgtcatc gatcaagcaa tcaaagtagt cagagatatc ttgcaacggt
    11101 tccaagtacg aggatataat gaagaaaaaa atgaaggaca gcttcgtcac atcattatcc
    11161 gtcgtggtca ttattcccat gaaatgatgg tcgttttagt tactcgtaaa gaaaaattct
    11221 ttaaatccaa ggaaattgct gaagctattc atgaagaatt gccagaagtg gtttcggtca
    11281 tccaaaatat caatgaagaa aaaacaaatg tgattttagg agacaaagaa aaagttttat
    11341 atggcagaga ctatatcgaa gatcagctat taggaaaaac ctatcgaatc tcagcaaagt
    11401 cattttatca agtaaatact caacaagcag agtatctgta ccaaacagcc atcgagtttg
    11461 ccgagttaga taaaaaagac attgtgatcg acgcttattc aggaataggt acgattggct
    11521 tgtctttagc agacagagtg gataaggtgt atgggatgga agttattcca gaagcaattg
    11581 aagatgcaca gttcaatgct ttaaataata aaatcgaaaa tgttcattat gaagtcggaa
    11641 aagcagaaaa agttatgaaa aaatggcaag aaaaagggat caaaccatct gtgatcgtag
    11701 ttgaccctcc acgaaaagga ttagatcatc attttattga atcagcaatc gaaatgtcac
    11761 cagaacgaat cgtctatatc tcatgtaatc cggcaacttt tgcaagagat gctaagttat
    11821 ttgcagaagc aggttatgcc gtgaagaaag ttcagcctgt agatcttttc ccgcaaaccc
    11881 atcatatcga attagtcgct tcattcgtta aaggagaata aaaatggacc aaaaagaaaa
    11941 aatgaaagaa ttgatcaaaa agaaacaagg tggaggccga tccaaacaaa tggaaacacc
    12001 taagaatgat cgtaaaaaca tgagaaaagg ccctaaaatc tttaataaat agacgtgcta
    12061 aagaaagact aagaagtgtt aacttttgtt attgtttctt agtcttttta gttagtctac
    12121 attattttgg cgatttgcca ataaggactg ttgagttgtg caaaatcaaa tctgggtcag
    12181 aatgaacgtc attctgtctc agatttttcg ttatttcaat ttgattaact gattgatcaa
    12241 aaagattttt attttcatat tctcaaatga ttcaacacta aatatacatg tttgacgcgg
    12301 aaattattat tcgattttgg cggcatcagg ggggatgacg acgtagcaga cccattattt
    12361 tactcaagaa aatgtccgtg cctattattt agaatacgat agtgaacgtg ctgggagttt
    12421 tgttctttta gagaaagtct ctgaagataa attagttgtc ttagattttg actgtaacga
    12481 aaaatggcga aatggaagag caccagcaag tgattgaccg gatctacgaa gcgacacgat
    12541 atctgccttt ggatcgtttg tgcttaagtc ctcaatgtgg atttgcttca acagaagaag
    12601 aaaatgtttt gtttgaagaa caatggaaaa aatttctttt gtcaaaagca ttgcagaaga
    12661 agtttgggga aaatcattaa atcatctagg agagcttgat aggaaataac actatggtat
    12721 aatcagaatg cttaatacta actactgagg gagccttatg aaaaagaaaa ttcttaaatt
    12781 tatcggatgg ttcttcggaa tcattcttgg gatcgcgttt gcaatctttc ttgccttcca
    12841 gctttccccc agacctggag cattcttgat caaccatatg ttttcaaata ctgttcaaat
    12901 cactgataaa aaaacgttcg atcaagcaga aaaaaacgtg gcgaaaaaaa cagatttgac
    12961 gtatgagtcc aagtataaag agaatactta tgacatctac tatccaaaaa acagcaaggg
    13021 gcctgttcct gttctatttt gggtacatgg tggaggattc gtcggcggag ataaatcggg
    13081 tgtcaaagaa tttgcgacaa aattagtttc agacgccaac atcgcagtgg ttgcgatgaa
    13141 ttatgaatta gcgcctgatt cagaataccc taatcaagtt cttcaagtga atgaactgat
    13201 caaagattta ttagctgaag aaaaagacga caaattgcta gacatggaac aattattttt
    13261 tggtggagat agtgcaggtt cacaaatcgc attgcaatat gcagctatac aaacgaatga
    13321 tgcgtatgct aaacaaatga aaatcaaaca gctacttcca aaagatagtc tgaaaggaac
    13381 catttcttat tgcggacctg ttgatttgaa gctaacagca cagcagcatt cggatgatcg
    13441 atttatgaag tttttcgtca aaaccgttgc gtggtcgttg attggaacaa aagattggaa
    13501 aacgagtgag caattgaaag aagctagtgt ggctgaccat gtttctaagg aatttccgcc
    13561 aacgtatata actgacggaa atgcatactc attccaggag caaggtcaag cattagaaaa
    13621 tcggttgaca gaattaaagg tacccgtgca atccttgttt tatacagata caaaaaaaga
    13681 gattacccat gaataccaat ttgattacac gctaaaagaa tcgcaaaatt gttaccagca
    13741 gacattggat tttgtgaata agtataagta agaggttgtg aaaaagccgt ttcgttttga
    13801 gtatgaagaa acgaagcggc ttttcacaac ctctctttca ataccttttt gttcttggga
    13861 taaaactttc cctaacaatc aatcgtgcag gataagtcaa aatgattggt tcgtcccgta
    13921 taccgtcaat tcgctccaat gccattcgaa cagctgcttg cccaattgat ttcgcattga
    13981 tttgaacact ggtcaagcca ggggagaggt ttgcgattgt ctcgatatta tcaaagcttg
    14041 ctacaacaat ctcttctcca atttggatat ttttttgttt caatgcccga tagacaccaa
    14101 ttgccattgg atcgctcccg acaagaatgg cagtaggaag aggggattca ttttctaata
    14161 gattagtcgc aagtacctca ccttgttttt ctgtccagcc acctagatga taagacatat
    14221 atttttctaa gtgatgatcg atcataaaat gctgataggc acgcatcctc ttttcattta
    14281 tagaaatctg cttcccaccg aattcatcca tatcgacttt atatcccccg atataggcta
    14341 ttcttttatg accattttct aagaacaatt ccagtacggt tttagtcatc cgttcaagat
    14401 ctgcatacac catatcgacc tcttgctgga tgtcagggtt atccacgacg actatttgca
    14461 tgttttgttt taacaaggcg gtaatcgatt ctttcgtaac agtcccaaca acgatcaccg
    14521 cacctaactg atctagatct tcccattgtt taggattatc agatagatta tagattcgat
    14581 tcatccctaa atgcagacgt cgagcttctt cttccatccc tactcgcaaa tgtttgtaat
    14641 agatatcctg ttcttcgctc gctttgccga tcgtagagat cacaccaatc gaaggcattc
    14701 tctttttgat atatttacga gtactgatgt cataatccaa cgcagcagct gcctctacta
    14761 tccgttgccg agtagtctga gccacatgga tcgtctgatc gttattcaac acacgggaaa
    14821 ctgtagcagg agagacattt gcctttcgtg cgacatctct aattccaacc atttttacac
    14881 actcctcaaa aaaataaaaa atttttattg acaaaattgt ttagtaaaca gtatacagga
    14941 attataaaaa aaagtaaacg gtttccgttt tgttgggagt gaaatgatgg atattacgca
    15001 tagaaaaaca gataaaaatt tcatcttatt tgatgaaaag agccgagtct tccatttacg
    15061 aaatacattt ctttcttatc tgataaagat agaagaaagc aatgtattgg cgcatattta
    15121 ttttggcaaa ccagtcaagc agtacaaaga caataaaaat tatccccgaa gagatcgtgg
    15181 attttcagga aatgttccct taaatcctga ccggtctctg tcgaaagata cactaccgca
    15241 agaatactct tctcatggca gcatggactt tcgaacgccc gcatcgatca ttcagcgcaa
    15301 gaatggatca gacttactgg atctgagata tgacagccat tatattacag atgggaagcc
    15361 agagatcgaa ggcttgcctc aaacttatgt gatggacaag tcagaggcac aaacgttggt
    15421 gatttcactt aaggatagag aaacagcgat ttatttcgat ttgttctata ccatatttac
    15481 tgatagagca gtgatcacac gatctgtaaa gataagaaat gaaaccggag aaacaatcaa
    15541 actagaaaaa gcggctagtt tccagctaga ctttgcccat actaggcgat ttgatgaagt
    15601 cattgctttg ccaggagcac atgtcaatga aagacagatt tcacggcaat ctgttttatc
    15661 tggaacaaaa gtatttgaaa gccgtcgagg aacgagcagt caccatatga ataactttat
    15721 tgctttagtt catcatcata cgacagaaaa tactggagaa gcgatcggac ttcaatttgt
    15781 ttattccggc aaccattctt tcgaacttga aaaagatcag atcaatcaat tgagagtcgt
    15841 cggtgggatc aacagccatc gtttttcgtg ggaattgaat gcaggccagt cttttcaaac
    15901 acctgaaatg atcctttctt actcaagtca gggattgaac aaaatgagtc agatccacca
    15961 tgagttgcta cgtgaaagaa tcgcaagagg tcgtcatcaa tttgctgaac ggccgattct
    16021 ggtcaataac tgggaagcca cttactttga cttcaatagt gaaaaaatca aggcgatcat
    16081 tgatgaagca aaggagctag gcatcgaaat gttcgtatta gatgatggct ggtttggaaa
    16141 aagagatgcg gacaattctt cattaggaga ctggttcgag tatgaaggca agttgacaaa
    16201 tggattacgt gaaatagcag attatgctca tagcaaagga ttgaaatttg gtttatggtt
    16261 tgaacctgaa atgatctcgg ttgattctga actgtatcga acacatcctg attttcttat
    16321 gcaggagcca ggtagaatgc cttctgcttc cagatcacaa catgtactgg attttacaag
    16381 gttagatgtt cggcagacga ttgagaaaca aatgagaaag attttagata cgattcctct
    16441 tgactacatc aaatgggata tgaaccgttc gctttcagat gtctattcga ttacccttga
    16501 cccacagaga caaggagaag tagcgcatcg ttatatgctc gggctttatg aactacttga
    16561 acatttgaca actgattatc cggaaatctt atgggaaggc tgttctggtg gaggtggacg
    16621 gtttgatgcc gggtttattt attatatgcc gcagtcatgg acaagtgata acacggatgc
    16681 agtggagcgt atgaaaatcc aatatggaac aagccttgct tatccgattt cgtccatcac
    16741 agctcatgta tcagctgtac caaatcatca gaccgacaga tctacttcgt tgaaaacaag
    16801 aggagaaaca gctatgagcg ctgtttttgg ttatgaactg gatttgacaa agctgtctcc
    16861 tgaagaaaaa aagcaagtaa aagaccaaat tatatcctat caaacgattc gtccggtgat
    16921 ccagtatggc cattattatc gtttggcgag tccttttgaa gaaaatattg ccgcatggat
    16981 gtttgtgtct cctaaacaag atgaagctat tgtgttttta gggaggatac tagcttcagc
    17041 tcaaccagcg ttccatgaag tatatctgat ggggttagat gatgaggcac tttatcagga
    17101 acagacctcg aagcggatat tttcgggggc cgaattgatg acagttggac tttacttccc
    17161 cgattttcaa ggtgatttcc aaacagaact gcttcatttc aaaaagttat gagagagaag
    17221 gaaaaaagta tgaaagcaaa tataaaatgg ttagatgatc cacaagtttt tagagtgaac
    17281 caatcaaatg cacatagcga tcatgtttgt tatgcaacaa gggaagaagt agaaaccaca
    17341 tcctccctta aacagtcttt aaatggatca tggcgttttt gttattcgcc aaatccttcg
    17401 gtacgaccaa aaacatttta taaaaaagat tttgatgcct gttcttttga tgaaatcact
    17461 gttcctagtc atattgaatt agcaaattat gacaagatcc attacatcaa tacgatgtat
    17521 ccgtgggagg gacatgtatt tcgccgtccc cctttcacga caagccctga gaaagaggcg
    17581 gaagggatct tttcagctgc taaagacaat ccggttggtt cgtatttgac gacttttcga
    17641 ttagaagaag gtctaaaggg aaaacaaatc tcgattcatt ttgctggtgt agaacaagca
    17701 atgtttattt ggttgaatgg tcattttatc gggtatgccg aagattcgtt cactccgtct
    17761 gaatttgatt taacacctta tttggaagaa gaaaatattc tagctgtgga ggtttacaaa
    17821 agaagtacag ctgcattctt ggaagatcag gactttttcc gattttttgg gatcttcaga
    17881 gatgtcttct tagttgctcg aggacaaaac catttagaag atctttggat caaacctcgc
    17941 ttagatgaca aatgtcaaaa tggcaaactt gaagtagatt tgtcttttga gcaagtagcc
    18001 aaaaacagtc agctgaatgt agcgatacta gatcctgatg ggaaggaaat ctatcaacag
    18061 caatatccgt taaaagagca aatgacgatt cgcatcgatg gatttgaaaa tgtggcactg
    18121 tgggggcatt ttcagcccaa tttatataag cttgaattgg agacgataga cgaagaaaaa
    18181 agagtaacgg agtatattga ctattccttc ggttttcgta aaatcgaaat caaagataaa
    18241 atcatctact tcaatggaaa acggatgatt atcaacgggg taaatcgaca tgagtggcat
    18301 ccgaaaaaag gacgcgccat cactatagaa gatatggaaa aagatttggc tatcataaaa
    18361 aatgcaaaga tcaacggcgt acggacatct cattacccca accagatcac ttggtattat
    18421 ttatgcgacc aagcaggaat ccatgtgatg gcggaaacga acctcgaatc ccatggctca
    18481 tggcaaaaaa tgggcgcatt tgagccttct tacaacgtac caggtgcagt acctcagtgg
    18541 caggcggctg ttcttgatcg agcaaaaacg aatttcgaaa cattaaaaaa ccatccaagt
    18601 attctttttt ggtctcttgg aaatgaatct tatgcggaag aaaacattcg acaaatgaac
    18661 gattacttca aaaaagtaga ccccgatcga cttgtccatt atgaagggaa ttttccaaat
    18721 ccagcattcg aagattcgat ttcagatata aaaagctgca tgtatgcgcc accagaagag
    18781 atcattgact accttgaaaa taaagcggaa aaaccgttca tcttatgtga atatatgcat
    18841 gacatgggga attcgttagg tggaatggat tcttacatga cgttgcttga tcgttatgaa
    18901 atgtaccaag gaggctatat atgggatttt aaagatcagg cgatccaagt gactgatcca
    18961 atcacaggca gagaagtgat gcgctatggc ggtgactttg acgataaacc aagtgactat
    19021 gaattctcag ggaatgggat cgtttttgca gatggacaag aaaaacccgc catgcaggag
    19081 gtaagatatt attatgaaaa atacagtaaa taaaagtcat atggatacgg aaaaagttgc
    19141 aatcgtcttc ggcgactgta cattaggtgt caaatcgggg aatacgcatt atattttttc
    19201 ttatacaaga ggcggactgg aatcgctcaa taaaaacgga aaagaatggc tataccggga
    19261 aacacttcca gttttttggc gagcaacgac cgataatgac agaggtagcg gatttggatt
    19321 taaatccggt atgtggttag gagccggatt gtatccaaaa gtcaaagaaa tagaagtact
    19381 ggtggacaat aaaaagatcg atttgccgat tgctccagta aataatcaat attcaagtaa
    19441 tgaatacgct gaaacggttg aaatcatttt tacactggaa atcaacacag taccaaaaac
    19501 ccaagtgaca atttcctacc aaatcgctgg agagggtaaa atgcttgtca atatgcatta
    19561 ttatggacaa aaaggattac cggaattacc cgcattgggt ttaaggttca ttatgccaac
    19621 gaaagcaaaa ttttttgagt atgaaggcct atcaggggaa acatatccgg atcggatggc
    19681 tggtggtgtc gaaggtattt ataaagtggc aggactcccc gtttccccat accttgttcc
    19741 ccaagaatgc ggcatgcata tggaaacgaa atgggtagaa ctaacaagaa atcaaacgtt
    19801 ggataatacg gataaagatc aagaagattt ttcgttgcga tttgaacagc aggatacgcc
    19861 atttgctttt tccgctttac cattcaaagc agaagaactg gaacaggcaa cacatataga
    19921 agaactaccg ccagaaagac gaacggttct tacgattttt gcaaaagtaa gaggagtcgg
    19981 gggtatcaat agctggggag cggatgttga gtcaccttac cgaatcagca gcgaagagga
    20041 ccatgaaatc ggctttatta ttcagtagca atctttagat tattatttga aaaacggagt
    20101 ttacaagaca aagctgcttt attctatatg tcatgaacaa gagagtaaaa aagttctcag
    20161 atacttttgt tcatgactat tttcttcaat aaaaccaatt gttatttttc tgaaaaacaa
    20221 agttaactat cagaaaatta aaaaaagttt cataaaaatg attg
//
LOCUS       DAKBVG010000026        20197 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000026 DAKBVG010000000
VERSION     DAKBVG010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 20197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.026"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(110..1423)
                     /locus_tag="OUD09_000724"
     CDS             complement(110..1423)
                     /locus_tag="OUD09_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HCT9692018.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCILMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLLGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFMGFMWILNVK"
     gene            complement(1668..1892)
                     /locus_tag="OUD09_000725"
     CDS             complement(1668..1892)
                     /locus_tag="OUD09_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9692019.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(1912..2334)
                     /locus_tag="OUD09_000726"
     CDS             complement(1912..2334)
                     /locus_tag="OUD09_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HCT9692020.1"
                     /translation="MESVYRQERKSNRKKGQTMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGVLTFNFSDPEGNYFVIGKAESSD"
     gene            complement(2476..3186)
                     /locus_tag="OUD09_000727"
     CDS             complement(2476..3186)
                     /locus_tag="OUD09_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9692021.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGDESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(3204..3700)
                     /locus_tag="OUD09_000728"
                     /pseudo
     CDS             complement(3204..3700)
                     /locus_tag="OUD09_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(3737..4189)
                     /locus_tag="OUD09_000729"
     CDS             complement(3737..4189)
                     /locus_tag="OUD09_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCT9692022.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(4324..4929)
                     /locus_tag="OUD09_000730"
     CDS             complement(4324..4929)
                     /locus_tag="OUD09_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HCT9692023.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(5379..5999)
                     /locus_tag="OUD09_000731"
     CDS             complement(5379..5999)
                     /locus_tag="OUD09_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HCT9692024.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(6001..6870)
                     /locus_tag="OUD09_000732"
     CDS             complement(6001..6870)
                     /locus_tag="OUD09_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HCT9692025.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANKAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(6893..7300)
                     /locus_tag="OUD09_000733"
     CDS             complement(6893..7300)
                     /locus_tag="OUD09_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCT9692026.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDRHKLAELYEQVGLKFSELHENDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(7319..8608)
                     /locus_tag="OUD09_000734"
     CDS             complement(7319..8608)
                     /locus_tag="OUD09_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCT9692027.1"
                     /translation="MSVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINDQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     ILLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(8635..8937)
                     /locus_tag="OUD09_000735"
     CDS             complement(8635..8937)
                     /locus_tag="OUD09_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCT9692028.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(8962..11055)
                     /locus_tag="OUD09_000736"
     CDS             complement(8962..11055)
                     /locus_tag="OUD09_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCT9692029.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLEEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(11544..12272)
                     /locus_tag="OUD09_000737"
     CDS             complement(11544..12272)
                     /locus_tag="OUD09_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HCT9692030.1"
                     /translation="MGLCLFALPTQGFAVAGGHGGGTSSGRSSGRGSFGGRSYGGSYF
                     GSRSYGRDSYGYYGPSTGPSSYEQTGFLALAVLSVGGVSAFSIVKNKRQKKRLKSEIL
                     SRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFAEHQKVLQKNKAD
                     GVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLISGSKTQRQYFSQ
                     IWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(12418..15039)
                     /locus_tag="OUD09_000738"
                     /pseudo
     CDS             complement(12418..15039)
                     /locus_tag="OUD09_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298916.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
     gene            complement(15153..16484)
                     /locus_tag="OUD09_000739"
     CDS             complement(15153..16484)
                     /locus_tag="OUD09_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCT9692031.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRVMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKANFGQLNELVPFGFAGMIASFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKNAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQSGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFVGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(16450..17928)
                     /locus_tag="OUD09_000740"
     CDS             complement(16450..17928)
                     /locus_tag="OUD09_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCT9692032.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLKNPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(18233..20068)
                     /gene="tdc"
                     /locus_tag="OUD09_000741"
     CDS             complement(18233..20068)
                     /gene="tdc"
                     /locus_tag="OUD09_000741"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HCT9692033.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEQVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     EQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKEIAKAMEKKLNEIIQ"
BASE COUNT         5827 a         4345 c         3395 g         6630 t
ORIGIN      
        1 ttcactcagc tgtcccttgg tattagctca aaaaaaggca aaatatgagg aactattttt
       61 tcgttttttt ctctaatact caggccagaa cactgatttc acaacctctt tatttcacat
      121 tcaatatcca catgaatccc atgaatacaa agaacagacc atacatgatg ggagatactt
      181 cttttcctct tttagctgct aacatggtaa ttggatagaa gataaaacct aatgcaatcc
      241 catctgaaat actataagtc aaaggcatgc cgatcaagat caagaatgaa gggatcgcaa
      301 tctctaagtt tccccattcg atttgtcgca aagattgtgc catcaatact cctaccataa
      361 tcaatgctgg tgcagttact tgtgaagtaa caactgatag taatggagaa aagaacaagc
      421 ctaacaagaa gaaaatccct gtagtaatag ctgtgatacc tgaacgtcct ccaacggcga
      481 taccagctga tgattctaca aatgcaccaa caggagaagt acctaataag gaacccgcaa
      541 gcatagctgt tgaatcagaa gctaaagctt tccctacacg aggcatcttg ttgtctttca
      601 tgaaacctgc ttggtttgct aatccaacta aagttcctgc tgtatcaaag aaagtcacaa
      661 gcaagaatgt cagcactacg acccacattt gaagagaatt gatgtcccct acatgtttta
      721 atgcaactaa aaatgttggt ttcaaacttg gtgctgcggc aacaatttca gaaggcattg
      781 gaatcaaacc tgtgaccaga cctaaaatcg ttgtagctgc catcccgatg aagatgccac
      841 ctggtacacg ccgaacaagc aagatagcag ttacgactaa tccaaaaatc gtcaaccaag
      901 tagaaccaac gttgagtgat cctaatccta ctaatgtaga ttcattcgaa acgatgatcc
      961 caccttcgct cagaccaagg aaagcaataa atagaccgat accgccagaa atcgcatatt
     1021 tcaaatctgc tgggatcgca tcaatgatca tttcccgcag cttgaagatt gtgatcaaaa
     1081 taaaaatcaa agaagcaacg aatacgccgg ctaatgctgt ttcccatgga atccccattc
     1141 ctaaacaaac agaataagca aaaaaagcat tgatcccaag agcaggtgcc gttccaattg
     1201 gataacgggc taataccccc attaaaatac agccgatcgc actggctaat gcagtcgcag
     1261 taaacactgc tccttcatcc attccagaag cacctaatac agttggattt acaaataaga
     1321 tataagccat tgagataaat gttgtaaatc ctgccagtac ctcacgtttt accgtggtgt
     1381 tcaatttttc tatttcgaag taggaaagta ttttttcctt catcttccat ccccctaaat
     1441 ccgaacatta taaataaagt ccgttaaaat ttcgatttat taataaaaca acgtgtttta
     1501 tcatatacta aaacaaaaca taagtaaaca aaaaattcgg attttaagca aaatccgaac
     1561 gttccatctg attaaatccc tttatctcaa agttcatgaa cattcacttt ggattacaag
     1621 aatttcttta ttgtgttaat aaagtttctt cattctagtc tcaaaaatca atagtgtgat
     1681 ttgattttac catttcgttt ctgttcttct ttgatgaatt cttttgtatc gacaggattc
     1741 agattattta gtccgccaaa acctgtatcg accatttttc cccagccttt tgtcttcttt
     1801 tttcctgatt tgagattctt tgctcgaagc ttctcactat gttttgctcg ccgatacttc
     1861 aaataacctt ctaatcttcc cattagatcc actgtttctc ctccttttta ttcaatcaga
     1921 cgactcagct tttcctataa caaaataatt gccttcaggg tccgaaaaat tgaaagtcaa
     1981 gacgccattg ttgtctgtga tttctcctgg ttcttccatg ttctcgtaga tagtttcaaa
     2041 ttcatccgta aaaaatacta aagaaggcgg tggtcctaat acctctggtg aaaacttctg
     2101 cacaaatact tttgggaata cgcctaattc taattctttt gagatgctga gtacaatact
     2161 cttaaattct tctggcaatt cgattgtttc agaaatttct gcaccaattt tttctacaaa
     2221 gaatcggcaa atcatctcta cgtcattgac ataaagcatc acacgtgttt tttgtatcat
     2281 cgtttgaccc ttctttctat ttgattttct ttcctgtcta taaactgatt ccattatatc
     2341 acgaaagcgc aaacaccttg tgtttgatac tattcaagaa aaataaagca atagtttcta
     2401 ggattgcttt cctccacaaa ctttttttac aaaaaaaaaa cggcaatgaa tgccgctctt
     2461 cttatttcga ttcttttata attctgcttc tgattcatct ttggttagtg tgtgtctaac
     2521 actagttacg ccaaatgttt gatcgatttt ttccttcaac gcttcgattg cctgtgcgcc
     2581 acgtgccgct tgttcagcat tgatggattc aatgaccagc accgctgctt gtgtttcttc
     2641 cgtcagcatc gttctttggg cttcacgcca gttgagacag cgacagatcg ctccttgttc
     2701 atcatagtag atgatttctt ccggaagtgc cggagcatcg gaatctgcac ctaaaggcag
     2761 gaatgattcg tcaccttggg cttttcctaa atgcagattt cctgcgatct tttcgatatc
     2821 ttctccgccg caaggtacgc caaaagacaa ggaaatacta ttatagatat ctacaagcgg
     2881 attgattggg aaaaattccc gttcttgatt tgcacgtttc agtaaagctt caatagaaga
     2941 acgtgcacct tttttagtct tgaatgtggt aaatgcctga cgccactgag caatgacttc
     3001 gttttggctg aatgtttctt ctgtaaggta atttcttgct tcttttgctg cctcattcaa
     3061 taattgatga tacgtttctt cactttcttt tgttacgtgg ttatcgatac catttacgga
     3121 taagatttcg atggttgctt caggaaatac ttcccagaaa ttggtatcca ctattacttt
     3181 tttcatctgt tcacacctct tgattagaat aattgcttga tgaatagata gtctgtttgg
     3241 ggatcatctc ccatataaaa aacatgttcg ctgtatttga taaatccttt tttacgataa
     3301 aaagcgagcg caacatcgtt gtgttcccaa acaccgagcc aaatggctgt tttttgcttt
     3361 tcttttgctt tttcaatagc tgtatctagt aaaaatgtac cgataccttg atgtttaaat
     3421 ttttttcgta catagattcg ctcgatttct aaaaaatgat cagccatttc ctcagattga
     3481 ctcgttcctg tatttagttt caggtaaccc gcaggctctt gatcgatact cactaggaaa
     3541 aaatctgttt ctttatcttt tagctcttct gtaagttttt ctagtgcata agcttgtgtc
     3601 aagtaatcag ctaaatgctc cggtaattat cttttccaaa tgtatctgta aatgtttcga
     3661 tactaatttg ttgtagtaag tctacatctg aaggcgtcat ttttcgaatt tcgtatttat
     3721 tcacggcttc ttcctttcaa tagatccttt tattgccttt tttgacaaaa ttccaatctt
     3781 cgctaacatt cttgcatact ttatttaaaa gatattccaa ttgttttgct tcttcgtcag
     3841 aaagaccatt taatgcgact gcattggagt agttgttttc tcgaatgatg aaaggataga
     3901 tttctgctcc ttttttagtc ggatagagtt tcttgatttt tttattttct ttatcttcca
     3961 aacgttcgat catcccattg gattctaatt tcttgattgc tcgggcagtc gttgtacgat
     4021 ctactttgat catttctgct agtttttctt ggatgattcc aggattttcg caaatacgta
     4081 ctaaataaag atactgtcct cttgttaaat cgacttcttt gaattcgata ttgctaatag
     4141 agtctagcgc tcgggcaatc attccgatgt ctcgaaggat ttcagccatt tcatcccacc
     4201 tttattttgt ttgtaagact agttcacctc tttttgttgc aaatgcaact atttttaata
     4261 aaaaaatcaa gttctttttt aataactagt aaagtatatt cttttcttct tttacgaaaa
     4321 agctcaatca caatagatct ggtttttgtt ttctaaaata agtaactgtt tcttcgttgt
     4381 tcgcttttct gatagctttg gcaactcttc tatactaaaa aatcccatgt tggaagtttc
     4441 attattctca ataaacttgg cctcttcacc gtggatcgca caagcaaaaa caagtttata
     4501 atactgaaaa agctgtggaa tatctttttg tttattcgta tcaaaaactg ctcgcaactg
     4561 agtagtttct actgttaatc ctgtttcttc atagacttct ttacgcacat tttcttcagg
     4621 agacaatcct atttcagcaa atccaccagg aagtgaccat tctttcgtcc gtttatcttc
     4681 gattagtagg accttgccat cttttttgat ataggcacga acatctactt ttggtgttgg
     4741 atgtccttct ccaatcgttg tgagcgcttt tatttcttcg aaagtttctt ttcctatttc
     4801 actgatcagt tccaatgaaa tcgtccggat ctcctcaaaa cgctctttat caaatacatc
     4861 ttttccataa tatagaccag cttgagaaag tgcgagtagt cttttgtatt gagccaactt
     4921 cttgttcatt ctcttccctt cttttttagc aaatgtatga ctatgtaatc aagtatagaa
     4981 gactggttta attacggcaa ataaatataa tgaaatctgc aataaataaa agaatgttaa
     5041 aaaactcgag aacaaaagac attcgacttc tctggggctg tgaataaatg gcgttcaaaa
     5101 gcttgcatct gcggtaataa gccccatcaa acgaaaaata tgacgagcta ttcttgtctg
     5161 gctgttctta ttaattgagg caggacagct ttttcacaac ctccccacta aacggtgttc
     5221 tcctaccaac tcctcggtca caagtcacaa tattcatcaa acatgagaag ctgtttcttt
     5281 gaaattgctc cttgtgactc ggagctgaac gacaggctca cgacctctcg gtaaacggtg
     5341 ttcaaaagcc aatttttcga tattaagcca tttctttgtt acttattgtc attgatttct
     5401 ttttctaatt tatccaatag tggagtgtaa tccacttgag tatagtctgt tacatcaaat
     5461 tcatatagtg ttcctaactg gaatttgtca tattttcggt cggcgatgat ctttttgaat
     5521 gcttctttcg taatatgatt ggtccctttc gttcgatctt ccaatacgtc accatagtga
     5581 taatgatcca tgatatagct caaatgacga tctgattcac gatcacgttt gtaccgacgt
     5641 tgccataaca cttcaaagtc tgctgttagg cgaatcgtga tcacctgata tccgtatcta
     5701 tctgctaatt cttggagtcg tgctttttgc ttatcactaa aaggatactc ggacacaatc
     5761 actttctttc ctgcttccat atacaaatca agtgctttgt aatagaaagg ataaactttc
     5821 gtcctttctt gttccgcccg ttcttccaaa ttgttaaacc cgatggattc tgcatagtat
     5881 tccttgattt catcgagcgt gatggcatac atatctggaa atcgttcccg tagtttattc
     5941 ataagatatg tttttcctgt accaggacta ccagccagaa gaatgaaata ctttttcatc
     6001 tcatttattt cctaagttaa ttggactttc caagttcata aacagtccaa tcttatgctt
     6061 gacaatttct tttactgctt tatttgctgg aatcacatta tggcgaagag acttgttgat
     6121 ttctgtttca ctaaaggttt ctttagctac tttcgtccag ttgactaaaa gctccgttcc
     6181 aacattgaat ttgcggatcc cgttattgat caattcagga taatcttctt ctttcacacc
     6241 tgttcctcca tggataacta gggggacatc aactactgca tcgatttctt tcagcaaggg
     6301 aatgtttact tctgtttttg atttgaattg tccatgattc gtaccgatag caatagctaa
     6361 tgcatccacg cctgtagctt ctacaaattt cactgcgtct tccggtcttg tatatacctt
     6421 gtcattttca gcgacatgga ttccttcttc tgtaccgccg attgttccaa gttctccttc
     6481 tacggaaaca ccgtgcgcat gggcatattc tactactgct tttgtcttca agatattttc
     6541 tttgaaaggt agatgggacc cgtcatacat gacagaagta tatccgctat cgattgcttc
     6601 tttgatatca tcaaagtctc gggcgtggtc taagtgtaag actgcatcaa ccatttcttc
     6661 ttctgcaata gctgtcatta cattgactaa cacatgcatg ccgatatatt tagcggtatc
     6721 gacacttgtt tgaataatga ttggcgaacc catttctttt gctgcgccta gcatggcagg
     6781 aagcatttct aggttatgcg tattaaaagc tccaactgta aaattcaact cttctgctgt
     6841 ttttgttact tctttcaatg ttgtatacat cttttattgc caccttatct ttttatttgt
     6901 ttcctttgat cgttacttgt tttgcaattt gtcgcatctc atccattttg cccatgtagt
     6961 aatcgatccc ttgatcatca ccagcacgag cagcatctgc acgcttctgg ttatagaggc
     7021 tcatcagttt tttataccca tctccgatcg tctgtttatt ttccaagctc ttttctaaat
     7081 attggatggc ttgatcgttc tcatgcaatt cagaaaattt aagtccaact tgttcataaa
     7141 gttccgctaa tttatgccta tcttgggtag tggaaatctc ttgtttcaat tgtgtaatct
     7201 ctgattgaat cttttcggtc gcttctggcg atagggcttt ttcttctttt gccagaactt
     7261 ttttcggttc tttttctttt ttcttaaaaa atccaaacat cattttccct actttcaatt
     7321 aaaacgcttt caaaactggg cttaagatcc aggcatataa gagtgctgcg gctactgtat
     7381 ctacatcggt tgctgtagca ttgatgaatc ccatcgaatt aaagatcgtt actagcaaag
     7441 cgggcaataa tgtaataaag aaaccatgag caataccgcc aatgatcgct cctcggcgtc
     7501 cgccaactgc gttgccaaat atccctgctg tccctccggc aaagaaattg gttagcatac
     7561 caggtaaaat catcgctaag ccgaacatgg gcaaagtaaa catggcaata atcgtaccaa
     7621 tcgttgtcgt aatgaaacct aaaatgacgg catttgggct ataaggaaag aaaactggac
     7681 aatctaaggc agggatcgca tcaggaacta atttcatagc tatccctcga aatgccggca
     7741 cgatttcacc taacagtaga cgtacaccag ctagcaatat atacacaccc actacgaatt
     7801 ggatggcttg taaaaaggca aacatgatat agttttgatc tcctgataat tcagaagcga
     7861 atggttcgcc tgcaaataaa acagtgatga tatatagcgg gaccatcacg accatgacag
     7921 acaaataagt atcttgcaga aattcgaaat gtgtcggcaa cttgatatct tcgatcgact
     7981 ttttattttc gcctttctca ccaaaaagct tagcaacacc ggcttcaaat aagtacccga
     8041 tcgtacaaaa atgacctaaa gcaatatcat ttgatccagt gattttacga atgatcggtt
     8101 gcgctactgc cggcatagca atggcgaaac aagctccgat aaaaccaccg accaagatca
     8161 atacgatccc tcgcagaccg gcaaaatagc caaagactgt cgtcatcgtt gccatccata
     8221 aaattgcttg accagttaaa aaaatgtact tccattttgt gaaacgagcc aaaataatat
     8281 tgaatacaaa aatggctaag aacgtcagtg caatatctct acctaaaccc aaatcattca
     8341 ttgcttgatc attgatagat tcaatagaag ggatgatccc ttgcatatga aatccttcag
     8401 taaagatttt cccgaaataa gttaaacttc caacgatgat gctcgaaccg gcactcaata
     8461 cttgaaatcc taggagtgtt tttaacgtgc cagagatgac ttgtcctgct gatttcttct
     8521 gcagtagtag acccaacata gcaatcaatg caatggtgat cgatgcctga gtcaaaatat
     8581 tttcgatgat aaaattgata acgctcatgt ttttaccctc ttttcttttt gtatttagat
     8641 aactccctta tcttgcagaa ctggggtgat tttttcttca atttcatttt ttgaaacaat
     8701 atttttcaaa tagatcatcg ccgttttagg atcaactgcg aacttttcga actgcgaacg
     8761 aaaattttct gctgtgatga tcacatctgg tttcatggaa actgcggaag aaatatcaca
     8821 atggtccagt ttggcttcta ctcctaattt gttcaaaaca tcctcagcag acatctgtgc
     8881 tgcaaaacta cttcctaaac ctgcgccgca tacgaataaa atgttcaatt tgttcatctt
     8941 tgttctcccc cgtatcattt attatttctc aactactgaa aaaagcagtt gtttgaaatc
     9001 ttcgatattt gtgcattcaa tcaatcgatc caacttctct tcgttgttga tcaattcaat
     9061 tagttctttc atgatgttca gatgggagta ggagtctact gctgccaaac aaaaaataat
     9121 ctttactgga tccatttcag gattaccgaa atcgattggt tgctcaatcg tagctacgct
     9181 gactcctaat ttattgaccc catcttctgg tcgggcatgg gctagtgcca tgtgtttgcc
     9241 aatcacgata tacggtccgt aatcattcac tgcacgaatc atcgcttcga catagcgctc
     9301 ttcgacaatg ttttctttaa ccaatggtct agcagctagt tcaatcgatt cctcccaagt
     9361 agtggctttt tctttgatca tgatattgct gtccgtcaaa atatctttta acataggctg
     9421 gatctccctt ttgttgattt ctaaattgtt tgtttcgaat aatttttcta acttgccata
     9481 gatgattcga tctactcggc caccactttc tttcatgaca tctattattt gagtgaataa
     9541 ctccgtcgat tcatgactgt taggctctaa ccgctgtagt tcttcatttc ttttcaaaaa
     9601 gtcttctata accaagatat tcgtctcagt taaaatcgga tcgacaacta aaagcggctt
     9661 atttcgataa ttcaattgaa aggtgctgaa aaccagattg acatccaatt tatctactaa
     9721 ttcgatttct tttgaactta aaactgcagt aatctcgatc tgaggaaact tttctttcag
     9781 attctctgct aacagatttc cagtagccat cccatggtta cagacaacga ctgatttata
     9841 aacataattc aagtctcgct tgattacact ggcgatcgtc gaaaagtgga tgacaagaaa
     9901 agctatctcg tcctccagta tcttgccacc aattacttct tcgatcgtag gaacaaaatg
     9961 agtgactgcg cggtagattg ggaaataatt tgttcggatg ttttctttca atggattcat
    10021 gacttggata tgattcttga ttcttacaat cagtgcagcc atgtgcttat agagattttc
    10081 gaataaggct tcttccttta gatggaaagg tacgtgtgtc tcgttctcta caaattggat
    10141 caattggatc gttagtaatt gagcttgtac ccactcgatt gaattggaga tatctctagc
    10201 attgaacgtc tcaatagtga aacgaagata atttctttct acttgcggga cattgtccaa
    10261 ttgaaattcc tcgatcatgt ggtcaataaa tgcagaaaag ctactgtctt ttatttctgt
    10321 atttttccaa ttaatggcag agataaattc atgtttcctg attcgctgga tccagatcaa
    10381 tgtaaaaagc aggatttgtt ttttataaat atcatcttct tgtttcaaca acttattgga
    10441 atagatctcc tgtatttttt tgatttcctc caaaggaaaa taatggagaa agattttttg
    10501 agccggtgtt aattgtgcat cttgattcat aaagaagtcg gtcattccca gattctgatt
    10561 gataaaatca tagatcatcg tacggataga tcgttctgaa cctttataaa ccagtccttg
    10621 ctttccataa ctgacgatct ctatcccata actgaccagt gttgctcgta accgtctcat
    10681 atcttcatct aatgtacttt tagagatttg aaattcttct tcttttttgt ttagatggat
    10741 cgggattcgg ctgtaagcaa cggctaaaac tagatcaaaa atacgttcgc gccgattcaa
    10801 aaagccgtct ttcacatctt ttatttcagt ttctaatcga gcttgctttt ccttttccag
    10861 cttcaacaaa aaacctttgt tgcgaattgt acggatctcc ggaagctttt tactgctcaa
    10921 ccagatattc gcttcaagaa cttcctgacg aatcgtccgc tgactgacat tgaactcact
    10981 tgctagatgt tttgctttta ctggatattg ggctgtcatg attctgtgaa gtatttcaat
    11041 tgttcttgtt ttcatagaaa tgatctcctt ctgatttcaa gtatatggtt ccgcttacat
    11101 aataaaaagt agatatttct gccacttgaa gtggaaagaa agtaagcaaa gcaaacaaaa
    11161 aataagccat atgctttttc atctcctcaa cgaaaaagac atacgcctat tattacaaaa
    11221 attttgccca tctatacaaa aaagaggttg tgaaaaagct gtcctgcatc aagtaataag
    11281 aacaactaaa ctaaaatagt gcttcctatt ttttgtttgg ttgttcttat tatcgcagat
    11341 gcaagctttt aaacaccatt tagtggagaa gctgtgacag agatgttcag ccttgagcga
    11401 taagaagcga ttttgaaaaa tcgcttccca tattttacaa aatcgtgcct tattgccgat
    11461 aggctatctc tggaacaccg tttattcaga aaaaggagct gcgacaaggc ttttgtcaca
    11521 actccgttca taaatatttc ctgtcaatcc agcataatcg gttgaataaa atcggcttgc
    11581 catctttttt ctttctcaga aaaatcaaaa taccatatct gagaaaaata ctgtcgttgt
    11641 gtcttacttc ctgagatcaa tcgcccatct tttcgatcga ctgaataatc acagcatctg
    11701 aaatcaattc ggatggcaaa actattttcg tggatctgtc gataatttcc tacttcctga
    11761 atagttattt ttttagtatg atttcttaca ccgtctgctt tgtttttttg cagaactttc
    11821 tgatgttcgg caaacagctt gtctgtataa cagcttctgg ctttttcagg agtttcttct
    11881 tcccaagcgg attggatctt catgaatatc ttttggattt ccaaaaactg tgctttcttt
    11941 tctttagccg ttcctgacat gcgggacaat atctccgatt tcagcctttt cttttgtctt
    12001 ttattcttga cgatagaaaa agcagaaacc cctcctactg acaaaacagc taatgcaaga
    12061 aaccccgttt gctcataact agaaggacct gtacttggac cataataccc ataagaatct
    12121 ctgccgtaag aacgactgcc aaaataactg cccccatatg aacgaccgcc aaatgaacct
    12181 ctgcccgaac ttcgaccgct agatgtacct ccaccatgac cacccgcgac agcgaatcct
    12241 tgggtcggca gagcaaacaa acataggccc actaacaaaa tgattgctaa ataattgatt
    12301 ttttcattgt cttcctcgtt atcttttatt acaaaaaact atttttatca tagccttgct
    12361 aaaaggaaga gtcaataacc agatgcataa actgtttttt tagcaatttt gtcttattta
    12421 gtcattgagt aacatcggac agatgtatgt gtcggcagtc cataattcct ctatcttcag
    12481 catgaacaag cttgcagaca agagatctgc gcttcctcca ggagagattt ggttttcctg
    12541 acaaaagtgc aacagtgctg taaatggacg atttggtaaa gcactttgac tagcagcatg
    12601 agcaagtttt tgcatttcca ctaacgtttt ctgatcactg cgatgcaata cgcatgtgtc
    12661 atctagcgaa ctcatcaaaa ctaacaacat atgcatttgt ttctgtacat atgaatcatt
    12721 atctagctcc ttagctgctt ggattgcaag cctgatatgc ggatacccta atatcgcttc
    12781 ttcatatgca ccatttacgc catatttttg cttcacccta tgaccatgag tggttttttt
    12841 atgacgatat tttgtatcag gaaaagcagc aagcttgctg acatcatcaa gtattttcga
    12901 taaagaaaaa ggctgttcca tactgatttt ttgtgcaatg acactgatca atagccccat
    12961 tgcccaaatc gctcctttat gggtattgat gccatttgta gcaatcagca ttgccttttc
    13021 cgcttttcgc ccaatctcag caattctttc tcttaactcc tgatcaatag cataatgcca
    13081 tgctatttga gccatttctt caaaaaaagg agttagcgtc ttagcagact tatccaaaat
    13141 gcgccgacct tcttttacgg cttttgcgat cgtatggaaa ttatcatctc ctaaaaccat
    13201 atcagctgcc tgtttgctta cttcactacc tttgattccc atagcgattc ctatatcggc
    13261 tcttttcaaa gcaggtgcat catttacgcc gtctccggtc attccgacga tttctccatt
    13321 tttctgcaaa gctttcacga ttcgcagttt gtgttctggt gtcgttcgag caaagacgtc
    13381 gacttttggt acttgtcgaa tcaactcttc ttcagacata agatccaaat cgatcccttc
    13441 taaaacttta gcagtatgtt ttaagccaat ttgttccgcg atcgcttttg ctgtatcttt
    13501 gtgatccccc gtaatcattt tcactgagat tcctgcttgt tggcattcct ttacagcttt
    13561 gactgcactt tcttttggcg gatcaatcaa cccagcaatc cctgcaaaag ttaattgggt
    13621 taaagactca tgcgtgagtt cctgtttcga atctactttt ttgtaagcaa atcctaaaac
    13681 acgctgacct tttttcgcaa acgctgtggc tgtttgaccc catgcttctt attctgaaga
    13741 agaaagagtt gatttagcca ataatacttc tggcgcacct ttgacgtaaa tcacccctcc
    13801 tttttcctcc gcatgcatcg ttgccatata tttgtattca gagctgaacg gaatttttgc
    13861 ttctattttt ttcaaaggca gatcggctgt ttcggtgtgg cgaatcagtg caagttctgt
    13921 cggattgcca cttaaatttt ctgttttctg ttcttctttc gtctttaatt cctgacaatt
    13981 tttcataata aacagtacgc gagtttcttc ctttgctgct acatccacaa ctgtcatttc
    14041 attttttgtc aatgtccctg tcttatctga acagatcact gtcatagaac ctaatgtttc
    14101 tacagaaggc agtcctttga tgatcgcctt ttcttttgcc atttcatgta ctccgacaga
    14161 taggatcatg gtcaacacag ctggcaatcc ttcgggtacc attgacacca ccaaagcgat
    14221 catagctgaa agaaggatat gccaatccat gccatagcgg aatgttgtaa aaacaactaa
    14281 gaacaagatc aaacaaagaa tgccttgaaa aatttgtttg ttcaatcggt tgatcttctt
    14341 gatcagaggc gtcgtttgtt gttcgaccga ttgcagtgct tgattgattt taccgatttc
    14401 tgtcgtgttt cctgtttcta caacgacacc gactgcagaa ccagattgta caagcgttcc
    14461 tgaaaaagcc atattttttt ggtcaccgat tgattcttct gctgccaaaa cggaatgatt
    14521 tttttcagat gcttctgatt ctcccgtcaa aatcgattct tctactagta gatcatgtac
    14581 ttcgatcaac cgaacatctg ctggaacgac gtctccggca cgtaagacaa tgacatctcc
    14641 ttttactagt tctttcgcag ggattttttc ttgcatcccg ttcgatagta ctatcgcttc
    14701 ttgtcccatc agctgcttca aaccattcaa cgattcttct gcttttcttt cttgccaata
    14761 actaacgagt ccgttgacga tcactacgaa taaaataata cttccttcga tgtattcgct
    14821 cgaagcaaat ttcaaaaaag aggcaatgat caacacgatc atcagcagat cagtaaagtg
    14881 ttttgctatt tttttccatc gttttgtctg ctgtcttgct tctatctcat tatatccttg
    14941 atcagtcagg cgtttctgcc gttcttcttg cgacaagcct tctgttgatg tttgatagtt
    15001 tttcaataca tcttctaccg tcaatctgta ccagttcatc ctatcccctc ctgtttaatt
    15061 gatactgtta ttatacgcag ggaagttttt tcaggcgtat cgattatttc aggggggtct
    15121 gaaataattc gttttgcaga atgtatctgg gcctaactga tatttctatt gcttattctt
    15181 ttatatacaa aatttaagaa tacaaaccag ataaccaata aaaaaggaat aaagcgtgtc
    15241 gtgctttcga ataacaagct gaaagcaatt gaagtaaaaa acaccaacaa agcaagatct
    15301 ttatatcttt gtgtagtgtt ccgtttcttt tctttaataa attgaacatg tgcacacaag
    15361 atcgaagacc acacaaatag aaaacaactc gttgtcatgc ctgccaaaag ctgaaaaaac
    15421 aatcgactat cagctacaaa aaaatgtaga caaagaccga caaaaaataa catccctgta
    15481 attaaaatac cgtgtaaagg tacgcctttc ttagaaagcg cacaaaacca atggggcaaa
    15541 cttttctctt ttgctgtttg atataaaatc ctgctagtac tgtagactgc actatttgct
    15601 gaggacaaag aagcaaccaa aatcattaga ttgacaattg ctgctccgct ttggcaatgg
    15661 atcacttgca gcatagtcac taaaggactt tcttttaaat ctaaacaatg ccatggaaca
    15721 acaaataaaa tcgacaaaat cgttcctata tagaaaaaga ggatacgcca aggcagacag
    15781 ttgattgcat ttttcaaatt cttttgtggg tcactcgtct caccaactgt taatccgatc
    15841 atttcaattc cagcaaaact gaataaaacc ataggaaatg aagcaatcat tccagcaaag
    15901 ccaaacggga ctaattcatt gagctgtcca aaatttgctt tgattccaat agactgggta
    15961 tgaaaatctg agacactcaa tcctataccc aataagataa aaaggaaaat ccccaagatc
    16021 ttgatgattg agaaaaaaga ttccaattca ccaaaccact tgactgcaac aaggttgact
    16081 gccattaaga tcattagtgc gctacctccg cttatcaaag ctggtacatg tacaaaccag
    16141 aaatggatat acaatcctat tgctgttaat tccgcaagtc cagctccaag ccagcaaagc
    16201 caataagtcc aattgacaaa aaatgcccac tttttgccta aataatagtc aacgaaatca
    16261 ctgatagagt taaatgtact atttgctaaa aataaatcac ccatgacccg catcaaacca
    16321 taaatagcca aaccgcatat caaataaacg agaataacag ctggccctgc tgatgcaatt
    16381 gcctttgctg aacctaaaaa taatcctgtt ccaattgcgc cacctaatgc gattaactgg
    16441 atatttcgat tagttagagt ccgtttcatt gccggttttt ccatcaagtt cacctttttc
    16501 tttttcaagc agcatggcga cttcctgttc ccaagaaggt ttctttattt tttcaataca
    16561 gagtggtact acaaacaaaa tgacactgat ggctgtgact gcaattagat tgatccatgt
    16621 cccagctttc atcgtcagtc cgacagaaaa tccgattgcc gcaaataaaa caataacgac
    16681 agtccaaact ccaagattgc tctttttctc tttttttcca attctaaatg gtcgattcaa
    16741 ctttgcatgt gtataacgca atctcaaaac tgcgatcccc atgatgacat agacgataca
    16801 gtagataaca gtagttgaat ttacgagcat caaaaatgct tgattgacgc caggaatcag
    16861 caaataaaaa agtgcaaaga ttgagataat gacagactga gtcaaaataa cggatttgga
    16921 tacgtcaaat tgattcgtct tccaaaaatt gaattttggt ggatacgcac cttttctagc
    16981 actttctgtc actgtctttg ctggtcctga ggcccaagca gataattgga ctgctacacc
    17041 aatgaagaca agtaaactaa acagattcac aataagatgt ggcaaaccaa gaatttggca
    17101 ataaatcgaa atagattgcg cgatattatt caattccatt tgtccttttg gtacgacatt
    17161 tgctacaact aaagcattca aaatattgac gaaaaatata aaaattaacg cggcaaatac
    17221 tccgcgaata tacgttttaa ctggattttt caaccgagtg atatacacag aggacatttc
    17281 gataccagta aaaataaaca tgatcggcgc aaaataaaca aatgaggatg cagtcgtagt
    17341 atctggtatt aatttgtccc atgaaaaact gcctaatgtt ccggttggta cgatcccaac
    17401 tttgatccac gcagcaaacc ctaataagag catcatcgtc aaaggcacgt acaatcctaa
    17461 ccaaacaccg acttttccgc cgatttttgc catatcaaat ttcaaattca aaaaagtgat
    17521 gatccaataa acgactaaaa tgacaactaa tgtaaatttg ctgttcaaac caagcggcac
    17581 attatcgata atattaccaa acaacggagc aagtgctgaa gcgaccatca ccatcccagg
    17641 aaacatttgg acccataaca gccatgagac aacgaacccc catttttttc ctagagcatt
    17701 cgatacccaa agttctggac cacctttttg gggaaacatt ccggcaaatt caccagagat
    17761 caatgagatt ggaaaagcgt acaataatac tgcaaccagc atgtaaaaaa tcatcgtcca
    17821 gctcgttgct gctacatctg gaatatttcg tacacttgct acaagggcgc aggtcattcc
    17881 gataaaggtc atggtcgtga tttgctttga tgaaccagta tctttcatct ttattccttc
    17941 tttcaatagc gacatatgtt tttgttttct tttatttttg ttcaagaaca gaaatgacaa
    18001 ggtttcatcg attcagtaca aaacccagta tcaccgaaac atccattgaa cgatcttagt
    18061 gaactgggtt tgatacatga aacgaacgat ccaacttata gaacgtttta taaaaaagaa
    18121 tatcttggcg aggaatatat gaaaaacagg gggaaataca attgttttga tgaagcaaat
    18181 aattgtctgt tctccatatg tctcttgtta ttctttcaca caaaaatcag ttctattgga
    18241 tgatttcgtt tagtttcttt tccattgctt tggcgatttc ttttgtataa aaattgaaaa
    18301 ttcgatcatc attcaaataa ggagtcatga tagccgcacg tagcagagtg attttctgtt
    18361 cttttttcca ttcttctttc gttagaccca ttctttcaac aaaacgaatc ggagaatcac
    18421 cgtaatcttc ttgtgtaaat gtagtatgag aagtaataaa ccgttcacca taaacatcgc
    18481 catccatata agaagaccga tcgaacattt tttcatttaa ttcattgata gcgttcaaat
    18541 ctgtacagcc ttgttctttg aatacccaat tgaccatatt aaaatcagga tgattcaatg
    18601 gatagacttc gattgttttt cctttaacag taaaagtcag atgatcgaga aattctctga
    18661 atctttgagc tgcctcgatt gaagccccaa tcaattgacc atatccggtc acattcaatg
    18721 gcaatacacg atgtgctgtc cagactgcgg cggcggttgc tccagctttt gatccttcca
    18781 aaatataagc tcctaacata tctggagcct ttactgattt ttcaaacaca tatggcgcga
    18841 aataagaaat gatatttctc atatttttat gtttgattac gattcctcca gcagcataag
    18901 ggacatagcc catcttatga ggatctatcg tgacagaatc cgcttctgaa atagctctga
    18961 acccttcata tacctctttg tcaatcgtta cgcagtggtg gaaaacatga tgttcttcaa
    19021 agaattcagc taaggaagca tacggcacaa attctcctgc ttcgtttaga aacagtgaac
    19081 gcgcatagcc gccatatgcg gcatcaacat gtaaatagaa atagatccct tcatctttta
    19141 acctttctct taattggacg attttatcta cactgtctac ttgaccttct tctgtcgttc
    19201 ctacgacagc aaccacacct agaattggga ttttttgttc agctaattca cgaattgttt
    19261 tttccaaagc attaatatcc attcgataat cctcttgaac tgggatagca accatctgat
    19321 ctaagcctac tccgcaaata tctaacgcct tcatccaaga atagtgtttt gtttgaggca
    19381 ccagccattt ccctaatctt tggatatttt ttccgctacg ggaagaagct gctttgacct
    19441 catccatttc ttcgtccgta aagctttcgg tcatttccaa aatttcttca acagataaat
    19501 tcaaaagttc ccactcagac atttttttca cttgttcagg cagcacttcc ttgacagcta
    19561 aaggaatcga tttgatgcag cgggcgtacc ataatccttc aagattagca atagaaccat
    19621 ctgctgcaat gtgtccccat ccgtctgtca tattgaataa tgaagcaaaa tcctgtccta
    19681 cttctgcttc catttgcgaa gttgccatag aagattccaa tgcaacatta tttgggttcc
    19741 acagcatcgc atagttgtaa gctaataaag caggcatcaa tgtttcagca ttcatttgtc
    19801 cccagtaacg gcctgctgaa tgccatggga tcgatcctgc gcgtaatcgc tgactgactt
    19861 catccaagac ctctatcata tggtcgcggg tggctagata atctggagtg agcttatctc
    19921 cttcgctaat tgcaggcata tctgaaggaa tataattttc tctccatcca agatgttcat
    19981 ctaccatctt gttcaacaac attttataaa ctggtccatt ttcagctttg tcgcctataa
    20041 agacggcctt gatatccata tctttcattt ttcttccctc catattttgt tggtcaatgt
    20101 ctcgcagata catgtgttta tctgtaacac ctcttcacta tagcgccaaa aatgagaaat
    20161 ttttttttat aacatcaaat ccacgtagat ttttaca
//
LOCUS       DAKBVG010000027        19741 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000027 DAKBVG010000000
VERSION     DAKBVG010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 19741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.027"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(<1..640)
                     /locus_tag="OUD09_000742"
     CDS             complement(<1..640)
                     /locus_tag="OUD09_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCT9692034.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEW"
     gene            897..4286
                     /locus_tag="OUD09_000743"
     CDS             897..4286
                     /locus_tag="OUD09_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCT9692035.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            4289..5710
                     /locus_tag="OUD09_000744"
     CDS             4289..5710
                     /locus_tag="OUD09_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HCT9692036.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            5707..7584
                     /locus_tag="OUD09_000745"
     CDS             5707..7584
                     /locus_tag="OUD09_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HCT9692037.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            7676..8518
                     /locus_tag="OUD09_000746"
     CDS             7676..8518
                     /locus_tag="OUD09_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCT9692038.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(8670..9545)
                     /locus_tag="OUD09_000747"
     CDS             complement(8670..9545)
                     /locus_tag="OUD09_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCT9692039.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            9752..10783
                     /locus_tag="OUD09_000748"
     CDS             9752..10783
                     /locus_tag="OUD09_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCT9692040.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            10872..11177
                     /locus_tag="OUD09_000749"
     CDS             10872..11177
                     /locus_tag="OUD09_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HCT9692041.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            11356..13371
                     /locus_tag="OUD09_000750"
     CDS             11356..13371
                     /locus_tag="OUD09_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCT9692042.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            13373..13657
                     /locus_tag="OUD09_000751"
     CDS             13373..13657
                     /locus_tag="OUD09_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCT9692043.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            13683..15056
                     /locus_tag="OUD09_000752"
     CDS             13683..15056
                     /locus_tag="OUD09_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCT9692044.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(15139..17154)
                     /locus_tag="OUD09_000753"
     CDS             complement(15139..17154)
                     /locus_tag="OUD09_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HCT9692045.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(17176..18390)
                     /locus_tag="OUD09_000754"
     CDS             complement(17176..18390)
                     /locus_tag="OUD09_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCT9692046.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            18611..19555
                     /locus_tag="OUD09_000755"
     CDS             18611..19555
                     /locus_tag="OUD09_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCT9692047.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
BASE COUNT         6697 a         3677 c         3717 g         5650 t
ORIGIN      
        1 tccattcaaa tggagttcct tgggattctc tcatgagatg cacaacaaaa tacataataa
       61 agaaagattg ctcatatggg ctttcttcct gaaacaaatt catctgacaa ttattcttga
      121 ccataatttc tttgatcttc tttttttcga ttggaaatgg caacgtgcgt cttccgtagt
      181 aacttaaaaa gaaaaaggta acaaagatac gcaactggag ctcactttta aggctagtta
      241 actgaaaacc ttggtcaacg atcactttta ataaatagct ttctaaaatt tgattgcatt
      301 gtttcaactt acgtgtaaaa gaagaccggc tgatggaaat tttttcacaa taatactcaa
      361 tcgttttacc cggattgaca ataatctcat ttaataaacg actgtttgta gaactttgcg
      421 acatttccat cagaacattt aaataagctg gtacattcaa atgttcaaga cgatacccca
      481 atttttgtga agattgaatc gttacatccg ttccccagcg gattttgatc tcttcgatat
      541 accttacaaa tgtagattct gaactaccaa ttcgtttggc aagttctttt ttagtcatcc
      601 acttgtcaga aatggacaga attcgaatga ttttttccat ctttctatag tttccttcca
      661 acaacctgat catatgtttt tctccctcaa cccttttctt tcaatgaaac gctcattaat
      721 cgaaggaatc aacttcataa atgaaaccaa ttggtatgaa aattggtgtg attcccttta
      781 gcgcccttcg tataataatt atcatattaa aataaataaa attatgtaac gcctgtttat
      841 tttaataatt ttcactattc acgtcaaaac agaaaaaaag aaaaaggagg ggaaaaatga
      901 cgaccacagg gaagaaactg aaagttattt tcatgctgat aatattgagt ttatcaaact
      961 ttgtgccatt atctgcaata gcagacacta cagatgatcc aacagtttta gaaacaattt
     1021 cagctgaagt catttcggat cagtctggaa aaaaagcact gaacatcaag ctaaatgcga
     1081 ataacaccag tgctgaaaag atagaaaaag aaattggtct agtcgaaaat tacttaagtg
     1141 atgtggaaag aaaagaagga gatggctatg cttatcaggt aaatagcggg aaaattacgt
     1201 tggaaatctc atcaaacact aaacaaacta tcgatctgag ttttccaatc gatccagcac
     1261 tttaccacag ccaggcaaac aagctgatcg tcgataataa agaatatgac attattgatg
     1321 agacagaaaa taagaaagat acagatgtgt cagtaccaaa gccagacgaa atagaagaag
     1381 aatcatcaaa agaaaacgaa aattctgtca gcccatttac attgcctaca ttatccttgc
     1441 cagctgtgag tgtgccatct aatcaaacga ttcctacaga atatacaaca gatgatcagg
     1501 gcacttatcc taaagccagt tggcaaccta caggaaatac aaatgttctt gatcatcaag
     1561 gcaataaaaa cggaacaaat caatgggatg gtataaattc ttggaatgga gatcctaatg
     1621 atcggaccca ttcgtatatc gaatatggag gaaccggtaa tcaagcagac tatgcgatac
     1681 gaaagtatgc aaaggaaaca agtacacccg gattgtttga tgtttatttg aatgctcgtg
     1741 gaaatgtaca aaaagatatc acgcctcttg atctcgtatt ggtcgtagac tggtcaggaa
     1801 gtatgaacga caataatcgg atcggtgaag taaagattgg tgtcgatcgt tttgtcgata
     1861 ctttagcaga tagcggtatc acagacaaaa tcaatatggg atatgtcggc tactcaagcg
     1921 aaggatatag ctacagtaac ggtgcagtac agatgggttc atttgattca gtgaaaaatc
     1981 aagtaaaatc cattacacct tcacggacaa atggtggtac ttttacacaa aaagcactaa
     2041 gagatgcagg aagcatgcta tccgttccaa atggacataa aaaagtgatc gttttgctga
     2101 cggatggtgt accaacattt tcctataaag tacagcgggt acacgcacaa tcaagcagca
     2161 attattacgg aactcagttt tctaatacgc aagatcggcc gggaaatact tctctaatct
     2221 caagaatcta tgatgcacct gaccaaaaca atctatccag aagaatcgac agtacgttta
     2281 tcgcaaccat cggagaagcg atggcactca aagaacgagg aatcgaaata catggtcttg
     2341 gcatccaact tcaaagcgat ccggcagctg gtctctcaaa agcagaagta gagtctcgta
     2401 tgcgacaaat ggtttcatca gatgaaaaag gcgatcttta ctatgaatca gctgatcatg
     2461 caacagatat ctctgaatac ctagccaaaa aagctgtaca gatctcagca actgtaagca
     2521 atggacaaat aaatgatcca atcgcagaac cattcattta tcagcctggt acactttcag
     2581 tcaagagtgt ggggacaagt cctacaacgg tcactccatc tatttccata gaaggaaata
     2641 ccatcaagag caatcagatc tatttaggaa aagaccaaga aatccaaatc cattaccaag
     2701 tgagaatcca aacagaaaat gaggacttcc atccaaattt ctggtatcaa atgaacggca
     2761 ggacaacttt ccagccaaac attgatacca atgaattagc tgaattcggt ataccatctg
     2821 ctaaagctcc cggagtcagt cttcacatca aaaagttatg ggaagaattt gacaacaatc
     2881 tagctgatcg tccagatcaa gttacttttg agattcaacg ggaacatacg acaaatgctg
     2941 cagcttggaa aaacggatat attcgaatca ttaaaccagc taaagataca acaaatacgt
     3001 gggaacgtgc agacattgac aaattatctg caaatagcgg agaaagttat caagagatat
     3061 tatcactacc tcaatacaat aatcaaggtc aagcattcag ttaccaaaca atcaaagaat
     3121 tacctgtacc aggatacgat tctcaacaaa tagatgcaat gacatggaaa aatactaaac
     3181 aattcacacc gttaaacttg aaaataacga aaaattcctc tacaggtgaa aaggatctta
     3241 ttggcgctgt tttcaaatta acaggagatt ctattgatac tttactaaca gatcatggcg
     3301 acggaaccta ttctcttcca gaaaatgtca aattgcaaaa agaaatgacc tatacgctga
     3361 cagaaacaaa agctccagaa gggcatggat taagcaaaaa gactacttgg gaaatcaaga
     3421 tcgcttctga tggtacggta accattgatg gaaaaacagt cactacttcc gatgatacga
     3481 tccagttgac tattgaaaat ccttttgttg aagttcctgt agcagtacgt aagtatgcga
     3541 tgcaagggac ggacaaagag ataaatctta aaggagcagc attttcccta cagaaaaaag
     3601 aagcaaatgg tacttatcag ccaattgaca gccaaacaac gaatgaaaaa ggtcttgcca
     3661 gttttgattc actcacacct ggtaaatatc gagtcgttga aacagctggt cctgccggat
     3721 atgatacttc gccgggaaat tatgaattcc aaatcgataa atatggaaaa atcatttaca
     3781 cgggaaaaaa taccgagatg acaaataatg tatggacgct cactcatcaa aatcgactaa
     3841 aagcgtttga tctaacggta cacaaaaaag aagacaacgg acagacatta aaaggagcaa
     3901 aattcagact gcagggacca gaaatggact tagaatcgcc aaaagatgga caagaaacag
     3961 atacctttct attcgaaaat ttaaaacctg gaacttatac gctgaccgaa acttttacac
     4021 cagaaggata ccaaggtcta aaagagccag ttactatagt tatacacgaa gatgggtcaa
     4081 ttcaagtgga tggacaagat catgaatctg ttctgtcacc aggagccaaa aacaaccaga
     4141 tttctttaga catcacgaat caggcaaaag taccattacc tgaaacggga ggaattggcc
     4201 gtttaggaat ctatctagta gggatgattg gttgtgcgtt ttctatttgg tatctttttt
     4261 tgaaaaaaga aagagggggc agctaaatat gaaaaaactt ggttggctta gtatgtgtct
     4321 cttcttgtta ctatttaaac cagcttttac tcaggtagca acagaaacag aaacagaaat
     4381 ggttcagatt actttacaca aattgctttt cccaaacggg caactgccga aaaatcatcc
     4441 aaatgacgga caagaaaaag ctttattaca aacgtatcga ggattaaatg gtgtcacatt
     4501 ccaagtttat gatgtcacag attcttttta ccatctacgg gaaaagggca aaacggtaga
     4561 agaagcacaa gcagagatcg caaaaaacgg tgcgtcttcc ggtatgttta ccgcagaagc
     4621 aacaactaca actcttaaca acgaagatgg tatcgcttct ttttctctgg ccgctaaaga
     4681 tcaagaaaaa agagataaag cgtatctttt cattgaatcc aaagtaccag aagtcgtcaa
     4741 agaaaaggca gagaatatgg tagttgttct tcctgtacat ggacaaaaca atcaaaaact
     4801 ttcaactatc catttgtatc ctaaaaatga agaaaacgac taccctgatc caccttttga
     4861 gaaggtatta gaagagccta gaaatgattt tacgattggt gaaaaaatca cttattcctt
     4921 gcatacgaca attcctgtaa atatccttga ctatcaaaag ttcgaattgt cagatagtgc
     4981 ggatgaagca ttaacgtttt tacctaatag tttaacgatt tcatcgaatg gagaaaagct
     5041 gacagaaggc tttgtcatac acaagaaacc tcacggattt gatgttttat tttcgatccc
     5101 ttcgttggaa aaatatgctg gaaaaaaact gaccatttct tatcagatgc agctaagcag
     5161 tacagcacag gcgaacaagg aaatcaacaa caacggaaca ctggattttg gttttggtgt
     5221 cagtacaaag aaagtctctg tatatacagg gagtaagcaa tttgtcaaaa tcgagacaaa
     5281 taaaccagat aaacgattag ctggcgcagt attccttatt aaaaacaaag caggaaatta
     5341 cctccagcaa acagccaacg gatacaagtg gacaaagaac gaatcagatg cgcttcacct
     5401 gatttccgat aaaaatggcg ctttttcaat ttccgggttg aaaacaggaa gttatcgatt
     5461 aaaagagatc gaagcacctt ctggttatat tttaagtgaa acagaaattc cgtttaccat
     5521 ttcaactttt ctttctgagg ataaagaggc ggacagtata ttgaaagtag tcaataaaaa
     5581 agaaaatagc cgtccatttc ttccaaaaac aaacgaaacg aaaaatacac ttttaggcgt
     5641 tgttggtatg gtattcgcaa gctttgcaat ctggttgttt atcaaaaaaa gaacaggagt
     5701 gaaaaaatga aaaatcataa aaaaataaac gttatgttag gagtcctttt ccttatttta
     5761 ccattactca caaacagctt cggcgcaaaa aaagtgtttg cagaggagac agcagctcaa
     5821 gtcatccttc ataaaaagaa aatgactgat ttacccgatc ctttaatcca aaacagcggg
     5881 aaagaaatga gcgaattcga tcaataccaa ggattagccg atatttcatt ttcagtttat
     5941 aacgtcactc aagaatttta tgcgcaacga gataaaggag cgtccgtgga tgcagcaaaa
     6001 caagcagtcc agtctttgac tcctggtaca ccagttgctt caggaacgac agatgctgat
     6061 ggaaatgtca ctttatcttt acctaaaaaa caaaatggga aagatgcagt ctacacgatc
     6121 aaagaagaac caaaagacgg agtgtcagct gccgcaaaca tggttttagc tttccctgta
     6181 tatgagatga tcaaacaagc agatggctct tataaatacg ggacagaaga actagatact
     6241 atccatctct accctaaaaa tacagtcggt aatgatggaa cgttgaaagt tacaaaaatc
     6301 ggtactgccg aaaacgaagc actaaatgga gcagaattta ttatttctaa agaagaagga
     6361 acaccaagcg tcaaaaaata catccaaagt gtcacagatg gattgtacac ttggacaact
     6421 gatcaaacca aagccaaaca tttcattact ggtcattctt atgacatcgg caacaatgac
     6481 tttgccgagg catctattga aaaaggccag ttgatcgtta atcatttaga agttggaaaa
     6541 tataatttag aagaagtaaa agctcctgat aatgcggaaa tgattgaaaa gcaaacaatc
     6601 acgccttttg agatcctggc aaatagccaa acaccagtag aaaagaccat caaaaatgat
     6661 acgtctaaag ttgataaaac aacacctcaa ttgaatggaa aagatgtcgc aatcggtgaa
     6721 aaaattcaat atgagatttc tgtcaatatc ccattaggta tcgctgataa agaaggaacg
     6781 caaaacaagt acacaacatt caaacttatc gatactcatg acgctgcttt aacatttgat
     6841 aatgattctt caggaacgta tgcttatgcc ttatatgatg gaaataaaga aatcgaccca
     6901 gtaaattatt ctgtcactga gcaaacagac ggattcacgg tttcagttga tccgaattat
     6961 attccttcat taactcctgg cggtacattg aaattcgttt actatatgca tttgaacgaa
     7021 aaagcagatc caaccaaagg attttctaac caagcaaatg tcgataacgg gcatacaaat
     7081 gatcaaacac caccgtcagt cgatgtcgtt actgggggca aacgatttgt taaagtagat
     7141 ggtgacgtta catcagacca aacacttgct ggagcagaat tcgtcgttcg tgatcaagat
     7201 agtgacacag cgaaatattt atcgatcgac ccatccacaa aagccgtcag ctgggtatcg
     7261 gcgaaagaat cagcaacggt ttttacaacc acaagtaacg gtttaatcga tgtgacaggt
     7321 ctaaaatatg gcacgtacta tctggaagaa acgaaagcgc cagaaaaata tgttccatta
     7381 acaaaccgtg tagcatttac tatcgatgaa caatcttatg taacagcagg acagttgatt
     7441 tctcctgaaa aaataccaaa taaacacaaa ggtacacttc cttcaacagg cggtaaggga
     7501 atctatgtgt atatcggtgc aggagtagtc cttctactga ttgctggact gtactttgct
     7561 agacgcaagc acagtcagat ttagtctttt tgcaagaatt ggaaaaagga gcagcggatt
     7621 acgcaaatgc tcctttttct tttattatca aacagtcatc acaaggagaa cacaaatgaa
     7681 accaaagaaa aggaaacttc tagatctgct gatgcttttc ttgcttctct caggaatagg
     7741 gatactagct tatccatttg ttagcgacgc gctgaataat tatctggatc agcagatcat
     7801 ctctcattat cagcaacaag cggtcaaaga aaatgaagaa gtcatggcta aaatccaaga
     7861 aaacatgaca aagaaaaacc agcaattagc aaaagaaggt ggaaatccag gtgcagatcc
     7921 ctttactaag aaaaaagaac ctgcgaaaaa ggatagaacc tattttgaga aacatacgat
     7981 cggtattttg acgatccctg agatcaatgt ccatttgcca atttttgatg aaacgaatac
     8041 tgtgctgcta gaaaaaggtg cctcattatt ggaaggaacg tcttatccta cggggggaga
     8101 aaatacacat gccgttattt ccagccatcg aggactgcct caagcaaagt tgttcacaga
     8161 cttaccgaaa ctgactttag gtgattcttt ctatatcgaa atcaatggac ggacactcgc
     8221 ttatcaagtc gatcagatca agacggtaga accgactgat acaaaagatc tgagaatcga
     8281 aaaagggcag gatttcgtga cactcctaac ttgtacacct tatatggtca acagtcatcg
     8341 cttacttgtc agagggcacc gggttcctta tcatgcaaaa gaagttcaaa aagaagtaca
     8401 gaaagtatcc gagaacaaac gatttttcct gtatggattg ttagctgcat gtatactgat
     8461 tttcctcctt cttatatatc tttttcgtca atcgttcaaa ataagaaaca gaagttagct
     8521 attcctcatt tctataatat atctattcga atcgttccgt aaacaaacag gctgaggttt
     8581 gaaaaagaaa acttcagcct gtttttatga tgatatcaaa gagagcctat caaacactat
     8641 ctttcttatt actaaatgag gatcttttct taagttttcc gatattcttt aagaaggcct
     8701 ttttgcatca attcttgttt gtttttcaag ttgcggatat tctcttcaaa attttgagaa
     8761 tatatcactg agaataacgt atttaatacg taatcgaagg caatttgaga ggagaaagtc
     8821 gctactttag caaaatcata ttcctcctgc gatataacaa gacacatatc agcctgtttg
     8881 gccatttctg aatgctgatt tccggtaatc agcaaggtag gggcctctac atgcttcaaa
     8941 taggtcatta ttcgttcata atgatggatg cgaccgctgt agctaataaa aatggcgcaa
     9001 tcctcgctag tcaaattggc tgcattccaa gaagaatcac tgtattcttc agccatgatc
     9061 aaaaacttat tcaacttgac cattttattc tggaatttcc ttgccgtgat ttgagaatca
     9121 ccttttgcaa aaagaaagat tcgctgtgcc ttgttcaata ctttaccgat ttgatctaaa
     9181 tcttcatgtt caatctgcaa ttgtgctttt tttattgact ggatcgataa atccgccatt
     9241 ttctttgcaa tttgctgagt gctatcttca cttgaaaaag gaaaattagg atcaatcgtt
     9301 tgattgaact gagcaatcaa ttgatgaact tcccgcccca gctcgaaacg gaattcttta
     9361 aatccgctga atcccatttt tttgcataaa cgaatgatag ctgaatggga agtatacgta
     9421 gcttttgcta attcttcaat cgtcatgtag actgcagctt cgatattcct acggatgtaa
     9481 tcgacaatcc gtttctctgt cgtggtaaac aactcttgtt ttgttagttt ttcttcaata
     9541 agcatgatct gacctccaaa ttctatctta ctgataaaca cttggttccg ctactatctt
     9601 tttgattttt atccttactt tgaacattaa tttcaccaaa ccgaatgata acaagtgttt
     9661 ttctgaactt ttttaccaga atagctatta taaatacttt ctgcgctttt ctaggatata
     9721 ctgaaaacat caataaaagg aggcaccact catgcttaca atcgcttatc ttggcaatgg
     9781 aaagagtaca aatcgctatc atttgccttt ttcaagaaaa cttacgaaca aaatcaatat
     9841 caaaaaaatt ttttccccat ctgaaccagt gtggaaaaca ttcgaagaca tcgaatatac
     9901 tgatcagctg tctgatcttt gggatgatcc tgagatccaa ttagttgtca tcactacacc
     9961 tagtcaattt cattatgaat atggaaaaat ggcactcgaa catggtaaga atgtactagt
    10021 agagaaacct tttgcagaaa cgtcaaagga agcagaagag ttatttgcat tagcaaaaga
    10081 aaaaggtcta tttatccaat gttaccaaaa cagacggttc gattctgact tcttgacagt
    10141 ccaaaaagta atcgaaagcg gaaaattagg ggatatttta gaagtagaaa tgcattatga
    10201 ttatttcaga ccggaagtac cagaaaatac gcatgaattt tctactgcga acagctattt
    10261 atatggacat gcttgtcata cagtagatca agttctttct tatttcggcg aaccagatga
    10321 tatcagctat gatgtacgtc aacttttagg gaaaggacga atgaatgatt acttcgatct
    10381 ggattttttc tatggaaata tgaaaatttc gataaaatcc agttatttcc gcatcaaaga
    10441 acgtccaagt ttcgtcgttt acggaaaaaa agggatgttc gtgaaacaaa caaaagatcg
    10501 acaagaagaa catctgaaaa tgttttacat gcctgatcat gaagactttg gtatcgattt
    10561 gcccgaacat tatggtgtat tgacctatat agatgaacaa gggagttatc atgaagaaaa
    10621 agtaatttcc gaggttggtg attacagccg tgtctacgaa gggatttacg agacattagt
    10681 taatggcgca gaaaaagtca tcaaagatga agaaacaatc ttacaaatga aaattttgga
    10741 agaaggaatc aaaagcattc aagaatcaga aggaaaaata tgacatattt gtaagaatcg
    10801 ggtttgtttt ttgacgcata aactgtctta ttgtacgatg aaaaagtata gaactactag
    10861 gaggaaccaa catgacaaat tatgacggtg aaaaacacaa acgattaaga ggttcaatgg
    10921 cccctaaaac agaagaagaa gtggctttag caggaactaa ttctggtgaa acagaagaca
    10981 aagaagaatc attagctggc acaaactctg gtgaaacaga aaataaagaa acagctttat
    11041 ctggttcaaa ttctgtagaa gaggaaacaa agcctaatga gcccaatcac tttgaaaaag
    11101 atatcgaaaa agcggttgaa gatgtcaaag aaaaatttga agaactgacc tcaaaaaata
    11161 aacatgatca tcattgatcg gacgaaatca attcataaaa gcaggagaca tttggtcacc
    11221 tgcttttttt ctgtatccat tcgatagaaa tcgttcggta cagtcgtaaa aaaaatccaa
    11281 ctgtaagttg taccaaacac ccctcgattt aaaagcgcta ccatactaga atataagtag
    11341 gaggtgaaca gcgctgtgaa ctacaaaatt agttatctcc tgaaaacacc aagagaaatc
    11401 agtctagaaa aagcagcaga gatcatcgaa gtaaatacaa gcgaggcgat caaacaatta
    11461 actccttttg ccgaatatgt ggatgatacc cgtatccgta taaagaaatt ccgagcaatc
    11521 gactgggcag aaaaattttt ggatattact cgatttgaag ctgtgtatac cgaacaagag
    11581 cgaagagatc ttctttattt gatggtttat tcgactgcaa cgtctgtttc tgtttttcat
    11641 tttcaagatt ttcttcaggt ttctaaaggg acagtcctgt cagatatcaa aaagataaga
    11701 aatgaattag aagtggaaga ttgtcagctg tcgtatacta ggaaaactgg ttttaggctg
    11761 ctagggtcag aagcaatatt aagaagagaa gcaaaaaatc gcactgctcg tttgatgcag
    11821 tcacttgctg gaagatttgg gctcacgtat ttaaccgcaa ccctttccat tgattattac
    11881 gccaaagtta gagatttgct agataaaacg atttcactat cagatcttcg attcgcacca
    11941 aatcgaatgg atgaattagc ttattatttg agtttgacaa ccattcgaat tggaaaaaca
    12001 tccatggaac tggtaaaaga tgctgaactg ttggcttctc ttgcagtcac tgacattagt
    12061 cgacaagtat tgactggcct tggctattcc gaacatcaaa attcggagat tatatttttc
    12121 tctctttgct tagcatcgat cgttgaagga gatgtccagg agcctgcact tgattttttg
    12181 ctggaatgtt cttcagaagt catccatcgc atggaattcc tgatggccgt agaatttgct
    12241 tcgtttcgtg aattattgat gaatgtcttt tatcacttgg tccctgccta ttttcgtatt
    12301 tcctattctt tttatttacc taatgtgatg atcgatcaaa ttaagcatca atatgcttcc
    12361 atctacgaaa tgacaagaaa agcattactt ccgttagaaa gacgaatagg gaagagcatc
    12421 cctgaagaag agattggttt tttcactata ctatttggcg gagagatcag aaaagcggac
    12481 gcagaagaaa gaaatagaaa aataagagcc gtgatcgtct gtccaagtgg cattagctct
    12541 tcattgatct taaaaagtga acttcagcaa ttattcccga tgatcttttt tacagagact
    12601 aattcagctg accgcttaca cgaggttgac gaaacttctt acgatattat tttttctact
    12661 gtacctctca cacttactga taagaaaaag tctctctatg ttctgcatcc aatcatgact
    12721 gacttggaaa aaaatcagct gatcaatcaa gtacaaagag attggctgat accaggattt
    12781 tcaatgcctt ctgcccaaga attgttgtcg gcgctactgc cttatatcga attaaaagaa
    12841 ggcgtagacg aagaaaagct ctatcaagta ttaaatcgta aaatgaacaa acttttagaa
    12901 aagaaggagg atcaacgtcc tatgctatct gaattattaa cagaagaaat gatccaatta
    12961 tcggatcaac caaaaaactg gcaagaagca atcacatttg ccgcacagcc gctattatca
    13021 gaaaataaaa tagaagaaag ttatatcaaa gctatgatcg agcgcgtgga gcagtatggt
    13081 gcgtttatcc atattggcga tcacatcgca ttacctcatg ctcgtccaga agatggcgta
    13141 aacgaagtag ggatgtcttt gctgaaactt caacaacctg tggatctgct tgataatcca
    13201 gcccatccca ttagtttgtt catctgttta gctgctatcg ataatgaggc acatcttcgg
    13261 gctttggcaa atttaacaaa gctactttct aataaacaaa atcttcaaga tttattacac
    13321 gcatcaacaa aagaagaaat attagcgatc ataagaaaag gagacgaatg atatgaaaat
    13381 ttttgcagta tgtcaaagtg gattaggaac aagttttatg gtagagatga acatcaaaca
    13441 agcacttgca gatcttggag cagatgtgga cgagtttgaa gtaagtcata cggatgttgg
    13501 aagtgtatct agcgatatgg ctgattattt cttcattgag cattctttga aagatacgct
    13561 aacttctata cctgatgaaa agctcgtacc tttgcaatcg atcattgaca gcgaagaagt
    13621 aaaagaaaaa gtaacgaaga tcttgtcaaa agaataaaaa tgattcaagg gaggaactat
    13681 caatggataa attgttgaat ttgtttgtgt caatcgtcag tcagccctcc atcctcgttt
    13741 ctttgattgc actgcttggt ctcgttcttc agaagaagaa attttcagaa gtgatccaag
    13801 gaactatcaa aacatttgtc ggattcctag tactgatcgg cggtgctggt ttaatcagta
    13861 attcactaac cccttttgct tcgatgttcc aaacagctct gcatactcaa ggagtagttc
    13921 ctagcaatga agcagtagtg gcgattgctt tgcaacaata cggtacacct actgctttga
    13981 tcatgctagt agggatgatc gtgaatattt tacttgctag attcacaaga tataaattca
    14041 tttttcttac aggtcaagca atgctttatg tttcttgtct aacagcagtc attttagtaa
    14101 gcgcggggtt tactgcaaat ttgtggatga tcttattagg cggggtcttc gaaggaacac
    14161 tattaactat tacgcctgct ctttgtcaac cgtttatgaa gaacatcact ggaaacaatc
    14221 aagttgcgtt agggcatacc ggaaatatcg gttatgcttc tagtggatgg atcggaaagt
    14281 tttttggaaa caaagaccgt tctacagagg acataaagat tcctaaaagt ttcagctttt
    14341 tgcgagattc tactgtatcg atcatgctct tgatgtcgat cgtatatgtc gcacttgctt
    14401 tgttagctgg tcctggattt gttgaaagag aactaagcaa tggagacaac gctgtaatct
    14461 ttgcactgat ccaagctgga acattcacag ctgggtttgt tatcgtcctt caaggggttc
    14521 gaatggtgtt ggcagaaatc gttccagcat tccaagggat tgctaaaaaa ctcgtacccg
    14581 attctaaacc agcattagat gttcctatcg tttttccata tgcacctaat gctgtattaa
    14641 ttggcttttt tgttagcttc tttgtaggaa cactatccat gtttgctatg ataggcttgc
    14701 aaactacagt tatcattcct ggggtagtag gacatttctt ctgcggagct gcttctggtg
    14761 tcttcggcaa ctcgactggc ggtagaagag gggcggtatt aggttctgct ttcaatagct
    14821 tattgattag ttggctgcca ttatttatcc ttccagtatt aggagatctt aaaatcgctt
    14881 cttctacatt tgccgatact gattatctag taccaggact tgtacttggg aaactaggag
    14941 aatacggtca aatagccttg acagttggaa tcatcgcttt ctttttactt gtcgtattat
    15001 ctagctttct ttttgccgga aaaagaacaa acattgtttc taaagaacag aattaagcaa
    15061 acttcaaaat aaaaattgct tggacaatat cagaagaaga tttaaagtct tctgatatgt
    15121 cgcaagcagt tttttgtttt atgcatttat ttttgaatag cttgttaaga caagtggctg
    15181 ttttgctaga agaggataac ttttaccatt cattttcact gttccgcctg ttctatcaga
    15241 agtaatcacg atcttatctt ttgttttttc aagcgaaatc tttccatctt tgataggtag
    15301 cgtacaattc agtgtttcaa atgaatctag attaggttgt atcataaatt cttcataacc
    15361 tggctttgtt ggatacaaac cgataaaata gcgtcctaaa agatagattg ggcttgctcc
    15421 ccatgcatgg cataagcttt ttccataagg atctccatac atactgtact tttcatttcc
    15481 tgtttgagta gggtcgtatt cttcccagaa agttaccgca tcatgagata acatccctcc
    15541 ccaataatca agaatcgttt ggtagacgat attcgtctgt ccgagtttgc ataaagcatc
    15601 ttgttcatag aatttgaaat atggagtagt aatctgtgtg atagtgtcat tcaataagac
    15661 attctttaag atcgattgct gttggtcttt gttagcaaaa tcgaataaaa cagcgaaaat
    15721 attcgcatga cgcgtcacat ttcttctccc agattcatag caatcgataa aagcgccaag
    15781 ttcttcatcc caaaagtagt caaataggtt ttgtttcaat agttgatatt tttcttgata
    15841 gagatgagta tgataatttt cgttctctcc atatgtttca gctaagatat ctccgcaagt
    15901 gatcatggaa tgataggctt tcagtaataa gacttgttct gcagcaagcg ttccttcttt
    15961 atccatttca gaccaatcaa taaatatcca atcttttgct ctcccataaa taaaccctaa
    16021 ttcattcgtt tgctccatca agtatctcat taatgattgc atcttagggt agacgatgcg
    16081 taaaaattca tagtctttcg tcatcatata gtggttttcg ataccaatca accacaagat
    16141 cgaatagtca acgatagtat tcatatgctg cttgatttca ttttgtcctc ttaatgctaa
    16201 tatcgttcgt ttattgattt cctcgtcaaa aaaaagatat tgattgatca tgaatgattg
    16261 atacgcatct ccagaccaaa tccagcgatc tcgtttgata ccgtcgataa aaaacaaatc
    16321 actacataac ttgaaggctt cttctgatac tgaccagatc tgattcagta aagaagaatc
    16381 gcttgaaaaa gcagcttgtt tcgaaagaga taaaaattca tgaaaaactt gaagttgaac
    16441 cattccagta gaaacctctg gtataaacaa atagcgaaaa gcgcgtttac gagctttcat
    16501 ttttcttgtc attcctattt gcttatagta gcaataatcg atatccatag cttctacatc
    16561 ggactctcca taacatagag tgatagattc gatatggtca tccagagata ttagttctac
    16621 acagccattc actgctcgcc caaaatcgaa taagattcct ccgccaatct ccgtttcagt
    16681 gactggatgg acgcattctt tttgataata gatttgattt ggatcttttg cgatatcgac
    16741 ataaagcgga ctccagccag ctggatattc ttcaataaaa ttacttgctg tccagccaat
    16801 atctgatttt acttcgtctc cctcgataaa catcgctggt agccctgatg tatgtcctac
    16861 aaagatacga atttttgctt taccagctgg acaagtcagc cattttccac atggatgttt
    16921 ttgtccatta atttctacat acccaactcc ttggatcgtc actttaaaca tggaaggttg
    16981 atcaagaaag taatatctct taaacttgac gttccgatgg caatcatcca tgtaccagta
    17041 cgctggccag tcaaaacctc gttcttcccg ttggaaattt tgtagcagtc catggcggat
    17101 ctcaaagtct ccaggatacc aaagccatct atctttatgt ttttcatttg tcattttaca
    17161 cgacctccat caaatttatt ttgttgtcaa ttgttctttt atttcttcat ctactcttat
    17221 cactgtcttt ctattcgtcc tgtgtactac tagaaggcta ataagcgcgc taatcaacag
    17281 caagatactg ccattgacga aaacagcaag tgttttactc ccaagagaag caaaagagag
    17341 aaagagcaaa ccatatggtg caatgaaagt acctaaatta gcgccaacca ataagattga
    17401 agtattgaaa ctctccgcgt acttgggcgc ttggatactt acttggttga ataagtaagg
    17461 aatgaataac gaaaaagaaa ttcctgttag gatcgcacca aacccaatca catacaggtt
    17521 tatcacagtg gcaatcaata caaagccgat tcccatcagt agtaaagaaa gtgtaagcat
    17581 atagcgatgt gcttttgtct gaagccagcc aaaacaaact ccggcagcca tacttgccaa
    17641 ttgcatgatt gttaagatat tcgctgcatt agtttcgttt cctagagaat aagtcgtcag
    17701 gactgtggct aatttaatcg atacagcatt atataccacg atgaccagca agagaaaaat
    17761 accataacta atcattttag gattcgtttt gattacttgt cttgttgggg ctgtgccatg
    17821 cgattgaagt tcagttgttt caggtgtagg aacaaacaaa acaaacaaga caagaatagg
    17881 taagctgatg gcgtagacta aaaaggtata ttgccaaccg aactgtacta gctgtcctgc
    17941 aacaaatgtc attaaagctg caccaaatcc ttgaaatgca ctttgaaaac caatcataga
    18001 ggctcgttca tctccgtgat aaaaggcagc aatcatgctg actgccaaag aattgatcaa
    18061 tccaaagcca atacctagtc cagctcttga agctaagaca atactgtaac tttgtgagaa
    18121 gagggggagg ataccacaaa tcgatgcaat cactaatccg atgatcacag tttgtttttg
    18181 tccgattttc ctagcaataa aactactaat caaaacaaaa atagtgacca taaaagaggg
    18241 agcagtcgtt aataattcaa cagcagataa agaacgcgtg gcataagtag ctgacatact
    18301 gggaattgca gacgtgactg caccagcaga agtcaaaata agagagactg acagcagaga
    18361 aaatttcatc atctttttat tcataagcat gttcctttca tttttgtaag cgcttatcaa
    18421 aaatatagta tgaagtggtt attctctcaa tagcggaatc acaggaagtt catgtcccaa
    18481 ccatagaaag tctttatgaa accaaaaata gctttgcata taatagcagt acagcgtgaa
    18541 ctaataagcg aaagaaaccg cttacaaaaa ggagggagtc aattggaact agctgaatta
    18601 gacatttttt ttgcgtaacg aagaatcaat cgaaactctg cagcgtgaac aaggaatgaa
    18661 tatcaatgat ttaaacttag cttaccatct tcagacaccg ccaaaaatcc accgagggga
    18721 tctatttcaa cagggaaata ttcatatcag taaacaccgt cgatatgcgg aagttcccga
    18781 gcatacacat gattttgttg aaatcaatta tatgtatagc ggtacatgtt tacaaattat
    18841 taatggagaa aggatagaga tggaagaaag tgacgtggtg atcattgata aagaagctac
    18901 tcaaagaatc gaatacacag ggcaagagga tattttaatc aacatcttat taaaagaaga
    18961 aaccttatct acagagatat tgaatcattt ggcaagcaca aataatctac tttcttcttt
    19021 tttgataaat gcatcaaaag aaaaagggga acatacacaa ttcattcatt ttcgcggcgg
    19081 aaaaacaagt cgtttgacgc atttgattga aaatatgctg ttgatttatt ttgatacata
    19141 cagtcataag atccgttcgt taaatctcta tacatcacta ttactgatcg aattgactca
    19201 tttattagaa caacaaagct tttcatcagc agatggatgc gcagaaaatg agatgcttag
    19261 agtattagat tatatcgata agcattttac ttcttgtacg ttaaaagaaa cagcagctca
    19321 ttttggctac aatccggtct atcttagtaa taaattgaaa atgtattttg atgcttcctt
    19381 tcaagaactg gttctaaaga aaaaattcga ctttgcgttg gaactgattc aagaaacgag
    19441 tttttcgttg gaagaaatcg ctaatcgtat cggatataaa caaacgactt ctttattcaa
    19501 attattcaag aagtattcaa catgtactcc caatgaatgg agaaataaac attaagaaga
    19561 aaatctttca ctaaagcata tgaaggaaaa ttgttttagg tgcgattgct gtcttattat
    19621 atcgttgcag cgaatttttt tggatttatt ttcagttcta aacttttggt tgtattatca
    19681 ggggatatag gtgtaaacat ttgtcattta tctgttatgc gtattcagga attcgtttcc
    19741 a
//
LOCUS       DAKBVG010000028        19432 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000028 DAKBVG010000000
VERSION     DAKBVG010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 19432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.028"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            <1..608
                     /locus_tag="OUD09_000756"
     CDS             <1..608
                     /locus_tag="OUD09_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9692048.1"
                     /translation="ENQLFAGDVLEIVLNDQAGEAAAKGVQNKPSTNDEIGNHNPSDT
                     PVVYHDAPPFAPATKIVVEGGLSFLAPSKLDCGQIKATGLTQEAFGQMDIQEKLWIYD
                     QRETKSPWILTIKLSQPFTTDAGFSMQDVLYFKKAEEYQLVNEEELEIVRGQNSTNVQ
                     TDYSEYLNNGRAFKLVLTKENQIAGNYSAEMTWTLADAPTE"
     gene            628..1656
                     /locus_tag="OUD09_000757"
     CDS             628..1656
                     /locus_tag="OUD09_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HCT9692049.1"
                     /translation="MNRIWNKSHRNNCSLLLILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILIRNSEKESSPNLKLKKTYADQSDGRNVIKANLQNPNAS
                     YMFGLTTSVEIYKKETKEPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKG
                     AWKEKRWNLHTDFEITDDAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFI
                     LKERRKKK"
     gene            1653..2411
                     /locus_tag="OUD09_000758"
     CDS             1653..2411
                     /locus_tag="OUD09_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HCT9692050.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPNEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QNGTDSTGTATQVQNFVQVTDKSGIYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGTPSTISGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEGKA"
     gene            complement(2497..3237)
                     /locus_tag="OUD09_000759"
     CDS             complement(2497..3237)
                     /locus_tag="OUD09_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCT9692051.1"
                     /translation="MKKYFIIEDNQFHSEWIIKELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            3298..4551
                     /locus_tag="OUD09_000760"
     CDS             3298..4551
                     /locus_tag="OUD09_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HCT9692052.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGFIALMVVTLL
                     FPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGLRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            4852..5100
                     /locus_tag="OUD09_000761"
                     /pseudo
     CDS             4852..5100
                     /locus_tag="OUD09_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            5222..5548
                     /locus_tag="OUD09_000762"
     CDS             5222..5548
                     /locus_tag="OUD09_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HCT9692053.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLQLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEF"
     gene            5616..6329
                     /locus_tag="OUD09_000763"
     CDS             5616..6329
                     /locus_tag="OUD09_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HCT9692054.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYLVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PIFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEEKEIVTLITCGDMDAVTRLVVQGELESVTP
                     IKDATDDMRSAFNLETKTF"
     gene            6639..7491
                     /locus_tag="OUD09_000764"
                     /pseudo
     CDS             6639..7491
                     /locus_tag="OUD09_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            7612..9477
                     /locus_tag="OUD09_000765"
                     /pseudo
     CDS             7612..9477
                     /locus_tag="OUD09_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
     gene            9491..10933
                     /locus_tag="OUD09_000766"
     CDS             9491..10933
                     /locus_tag="OUD09_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003243232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCT9692055.1"
                     /translation="MQSRFSEKFLWGGATAANQLEGAYDRDGKGLSVADAMPGGKQRF
                     AIIGSEEFDWTIDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPN
                     GDEGTPNEAGLEFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYE
                     RFAQTVLERYSSKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVA
                     SSKAVKIGHELRSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGE
                     YPAYTARTHKKYGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSE
                     GNLLGGVKNPFLEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDG
                     TIIDDYRIDYLRRHIEAMANAVADGVDLMGYTPWGCMDLVSASTGEMSKRYGFIYVDL
                     DDEGNGTLNRSKKKSFDWYKEVIASNGVNL"
     gene            11041..11742
                     /locus_tag="OUD09_000767"
     CDS             11041..11742
                     /locus_tag="OUD09_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCT9692056.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            11901..12380
                     /locus_tag="OUD09_000768"
                     /pseudo
     CDS             11901..12380
                     /locus_tag="OUD09_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            13227..13802
                     /locus_tag="OUD09_000769"
     CDS             13227..13802
                     /locus_tag="OUD09_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HCT9692057.1"
                     /translation="MKFTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            13802..14242
                     /locus_tag="OUD09_000770"
     CDS             13802..14242
                     /locus_tag="OUD09_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HCT9692058.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            14258..14959
                     /locus_tag="OUD09_000771"
     CDS             14258..14959
                     /locus_tag="OUD09_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HCT9692059.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIHQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(15447..15872)
                     /locus_tag="OUD09_000772"
     CDS             complement(15447..15872)
                     /locus_tag="OUD09_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9692060.1"
                     /translation="MKKLIVLFILSAGLLTLTGCNLFGGSDQSSDETTDSSVLKAEKA
                     DITNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQETDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKEATITFTKIGNYRLKDEDGNIYSL"
     gene            16246..17217
                     /locus_tag="OUD09_000773"
     CDS             16246..17217
                     /locus_tag="OUD09_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCT9692061.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKELLEKGVTAIFVSNYNMSIGAIECFNQEGIRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGEQAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            17249..18646
                     /locus_tag="OUD09_000774"
     CDS             17249..18646
                     /locus_tag="OUD09_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCT9692062.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEAFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHPEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            18805..19428
                     /locus_tag="OUD09_000775"
     CDS             18805..19428
                     /locus_tag="OUD09_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9692063.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
BASE COUNT         6632 a         3185 c         4059 g         5556 t
ORIGIN      
        1 cagaaaatca actttttgca ggagatgtat tagaaattgt attaaacgac caagcaggag
       61 aagctgctgc taagggtgtg cagaacaagc caagtaccaa cgatgaaatt gggaatcaca
      121 atccaagtga tacgccagtt gtttatcatg atgccccgcc ttttgcacct gctacgaaga
      181 ttgtagtaga aggtggtctc tcatttcttg ccccttccaa actcgattgt ggtcagatca
      241 aagcaactgg gttgacgcaa gaagcatttg gccaaatgga tatacaggaa aaattatgga
      301 tttatgatca gcgagaaaca aaaagtccat ggatcttaac gataaaatta tctcagcctt
      361 tcacaaccga tgcaggattt tctatgcagg atgtcctata tttcaagaaa gctgaggagt
      421 atcaattagt gaatgaagaa gaactggaaa ttgtcagagg acaaaattca acgaatgtgc
      481 aaacagatta ttcagaatat ttaaataatg gtcgggcgtt taaacttgtc ttaaccaaag
      541 aaaatcagat agctggtaat tactcagcag agatgacatg gacattagca gatgcgccga
      601 cagaataaag atcaaggaga atgattgatg aatcgaatat ggaataaaag tcatcgaaac
      661 aactgctctt tacttctgat ccttctcctt ggattcatcc tttggcagac acctgtccat
      721 gcctccgaat tcaattttgc ggtagatttt gaattaccgg ctaatcagat tgataagaac
      781 aaaacatata taaatgtaag gatggagcct gggaaaacac aggaaatccc ctacacggtc
      841 tttaaccgaa cagataaatc agtaacagtt gagacattga tccactccgc tacgacgaat
      901 tcaaacggtg tgatcgaata tggagaatca aaaagtaaat ttgatgaaac attaagttat
      961 gatatgaaaa acttagcaaa aacagtggga gaaataactg tccctcctca aagtgcggtc
     1021 actgagacac tttcactgaa agctccagaa aaagagctgg ctggtgtggt cgcaggagga
     1081 atcacgtttc aagaaaaaaa agagaacgtt tctaacaaaa gtacagttgg tgtcgtcaat
     1141 cagttttctc aagtgaaagc aatcttgatt cgaaatagcg aaaaagagtc ttccccaaat
     1201 ttgaaattaa aaaaaactta tgcggatcaa agcgatggaa gaaatgtgat taaggcgaat
     1261 cttcaaaatc caaatgcttc ttatatgttc ggcttgacta catcggttga aatctacaaa
     1321 aaagaaacaa aagagcctta tctttcaata aaaaaagaaa aaatagatgt agcacccaat
     1381 acaaattttg atttacttgt cccattagaa ggaaaaaaat tagctccggg acattatcaa
     1441 gcggttgtga agggtgcttg gaaagaaaaa cgctggaatc tccataccga ttttgaaata
     1501 actgatgatg cagcggacca attgaatgaa aaggatattg atttaaaaaa tcagccagaa
     1561 acatttgact ggcgatggct gatacttgtt ggagtcattc ttattggctt atttgctgga
     1621 ttatttattt taaaagaaag aaggaaaaag aaatgaaaaa aacaattgca ttaagtaccg
     1681 ttctagctgg aagtctatta ttatgtacac aaagtgtttt tgctgagaat aagagctcag
     1741 ccactacaga aggaacgatt tctgtaacaa aaccagatgc cgcaaatcct gaagacgttg
     1801 ttccagttcc tcctgttgat ccaaatgatc ccgatacgcc gtttacacct aacgaaccaa
     1861 atcagggaac atccggatta ctgacgattg accaagctcc agatcttgat tttggaacaa
     1921 tcaagttagg tacagcaaaa acagtatatg ctgcattaca aaacggaacg gacagtacag
     1981 ggacagcaac acaagtacag aatttcgtac aagtaacgga taaaagcgga atttatgccg
     2041 gttggaaact atcagtcaaa cgaacagaat ttattggtac aacatctgat acgaataaac
     2101 taactggttc aaaggtgaaa ttcaaaaacg ccgtgattcg aaatagcgca actaatagca
     2161 gtggaacgcc atctactatt tctggtactg gcgctgaagg aattgagatc ccagcagatc
     2221 aggcagtaga tctagtaact gcaggagcaa atgaagggat tggcacatgg atttattcat
     2281 taggagagaa tgtggaacaa gggagacaat cagtggaatt atttgttcct gtatccaatt
     2341 atgccgtaga caactacgtt tcaacattca catggtcgtt aacagatgct cctacagaag
     2401 ggaaagcgta aaatcaaatc aatgacttca gggagtggag ccgattcaag gccaactccc
     2461 tgaaatctgc tatgcttacg tctcagctct tattcatcaa ctcgaagcag ttttttggaa
     2521 gtcatggaaa cgttcctttg tcttttcaaa gatctttcga ccaacgagta aggagtcacc
     2581 attgaaaaat tctaacacat aatgagcaga atcaattttc tgcaattgcg ataagttgat
     2641 acagtaagat tttaatagta aaaagtaagc atacttttcc aactctgtct tcaattgttt
     2701 taaagaagta ttcactaatt tatcttctgt agttgttttg accataactt ggtttttcaa
     2761 acctttgata ctttcaatat ataaaatttc ccgaggatac aggacgagac ttccaccttt
     2821 ttgatcaata aagattttat cgacatttac gatttgctgt tctttccaat tgatctgttc
     2881 tagcacttga cgaatttgtt cagataaatt cctgtctttt ctgatatatc caatcggaag
     2941 gattttccga ttgatgatct ctactgcaaa attcgtataa gcggtgaaaa aaacaatatt
     3001 actatgtgca ttttttttac gtatttgttc ggcgacatca atacctgtga gtgattgctt
     3061 caagtcgata tcgataaaga aaacatcgtg attatcaata gtacccaact ggatcaactg
     3121 attgatttga gagatagaag tgagctgcac gatattctcg atatgatgat tgaccaattc
     3181 tttgatgatc cattcgctgt gaaactggtt atcttctatg ataaaatact ttttcatatg
     3241 caggctccta ttaaaaaaat atgtgtacat tttgaaacaa aacaatggag gaataagatg
     3301 gtcgcaacag tgacacttta tttgaccttt actttgattt tagatttagg tatctattta
     3361 ttcttttcga aacaactgct agtaaaaaac agctttggtt ttatagcttt gatggtggtg
     3421 acacttttat ttcctatccc taaagtgagc tttccttacc tagttggctt attattgatg
     3481 tttctttttt attttactac gaaaaacctg tttatcagct gtttgatcag taatcttttt
     3541 tttgctatca ctggactcag cttttttctg gtttttgatc tgatggatta tttaggaaag
     3601 agacatcttg gaacagcaag tttttttgca ctacttatcg catttatttt ctatctatta
     3661 gtaaaaagta tagacaaaca gacgagattt ctcaacttgc tttttactta tccaactgaa
     3721 cactattttg gaggtatttg tattttatta agctggggcg tgatttactt ggtattggga
     3781 agtgttcact tccgctcatc taattatctg cttctatctc ttttgtcgat agtcatcagt
     3841 tttttttctt tgtttttttc cattatgttg atcagtttcc aagtaaaaga aagacaacat
     3901 caggaatcgc ttcaactgta tcaaataaat gaagaatatt accatcatct agaggaattc
     3961 aaacatgatt ataaaagtct actgttttca ttaaaaatta ctcttcagga aaataaaaaa
     4021 gaaacgttgg aatttttaga aatcttagaa gatcattctg cagctatttt tgaaaaaccg
     4081 caaatacaac agctaacaaa tattgtatcc ccagctgtca ggggtgtctt tttaaaattt
     4141 ctagaaaaag cagaatcgga gaaaatcccg atccaagtag ttattcctgt cgaagtgaaa
     4201 acaatcccga ttgatctggt tgtttttatt cgctgtttat ctatttttat ttctaacgca
     4261 ttcgatcatc gtgtagccga tcagagtccg attacgatca atctgctgga agaaaaagag
     4321 ggattacgtt ttagtatagc gaacaaggca aatccgacga atttgacgct atcggagatg
     4381 gtcaaaagag gaaaaacctc taaaaaaaat ggcgggttag gtctatacag tgcgaaaaaa
     4441 atgttggatg tttacgaaaa tgcagaacta aaaatcgaat tttcaaaaca gaatcactat
     4501 ttttttgtag aaatgtatct ggcatctaga aagagcaaat cagcctactg acttactgtc
     4561 aaaggataag cttgaggaaa gtctacacgg agatagagac aacaacacag catattcaaa
     4621 agaaacagaa gcgtgatgac cgttgtcacc cttctgtttc ttttgctatg tagttaaaca
     4681 aagtagttga cacttccgct acttagtatt agtacgaaag tatacttcat gatactaagt
     4741 agataagaag tgaaaagcgg aacccacgat cgtcaaaatg ctaactgctt tgcttacacc
     4801 agataaaggg aagagcaata tcgcttattt taggcgtgga gaagcaagaa aatccctacg
     4861 atcacacaaa aaatgctgac ccagcagtta agagagttag aacaagatgg cattgtccat
     4921 cgaaaagtct ataaccaagt cccgcctaaa gtagagtact cgctcaccga agaaggaaaa
     4981 tcattaagag agattctagt agcgatgtct gtttggggag aagaacgtat cgaaaaacaa
     5041 aaagcctcag gggaagaagt cacattactg catacacatg gtggctacct aaattactga
     5101 tttctatgac atttatttta aataaaagaa aacaggagtt tttttaaaag catcatctat
     5161 tttttatgta aaaaaatggt atgattaatc aagtaagaaa tgattggaag aaggcaagca
     5221 aatgaatagc atggaaaaag ttaaccagtt tagagatgaa cgtaattggc gacaatttca
     5281 taacgaaaaa gatttggcta tttctatttc tttggaagca tctgaattgt tagaactttt
     5341 tcaatggaaa acgccggaag aagtaaaaga aacacaatta gaacgaatca aagaagaact
     5401 ggcggatgta ttgatctatt catacatgat ggcagacaac cttcagctgg atctagatga
     5461 gatcattgaa gaaaaattga tcaaaaataa tttgaaatat cctgtagaaa aaagcgcagg
     5521 tgtacgaaaa aaatacacag agttttagaa atgtgtcatt cggctgttgg aaagagcaaa
     5581 agaaaaacag taaaatgagt ctggagggag agaaaatgaa aaaaaaagga aaagcaaaaa
     5641 gatggctagt caatatcctc ttgttcctct tgttattagt aggactagct ttgattttca
     5701 atgaacagat caaggattat cttgtcagag agacaggaga taaatatgct atagcgaatg
     5761 taacaaaaga agatctgaaa aagaataacg ataaagatgt cagttttgac tttgatgcgg
     5821 tagaaccaat gacgacagaa ggggtcatga ggtcgcagat gagaggaaca gatctaccag
     5881 tgattgctag tatcgcagtc ccttcagttt caatcaattt accgattttc aaaggcttgg
     5941 ataatacgtc actactttac ggagcaggaa cattatctcc agatcaagaa atgggaaaag
     6001 gaaattatgc cttagcaagc catcgtgcaa cgaaccctga attgttgttt acaccgctgg
     6061 aaaatttaga gatgggtgcc aagatctatt tgacagattt agaaaatgtg tatacataca
     6121 aaacattttt taaagaaaaa gttgctccta cagataccca attactgaat gaagtcgagg
     6181 aaaaagaaat tgtcacacta attacttgtg gcgacatgga cgccgtcacg cgactagttg
     6241 tacaaggaga actggaaagt gttacaccga taaaagatgc gacagatgat atgagatctg
     6301 ctttcaatct agagacaaaa acattttagg atactgggac ataagtcgtt acagattaaa
     6361 aacagccaaa ggatatgaat cgataaggaa aaaccaattt gctgaaaaaa agaggaacgc
     6421 aaagaggtgc atctctgatt tggttgtttt ctagatagac ttgtgttcca gtttttttat
     6481 tttttatatg aaaaggcttg cataaataaa aattcgatgg tatgatgatt ttgaataaga
     6541 aatggattgt tactgtacgg caggcaaaac cagaattcag ctaagaaaca gtaggcgaat
     6601 tctaggtttt tttatttttc ttgagagggc gaagcaaaat gaaaattgat aaaattttga
     6661 ataacaatgt cgtcatttcg aaaaatggat tcggggaaga agtcgtttgt atgggaaaag
     6721 gactagcttt tcaaaaaaaa gattggggac gagatttcgc cagaagcggt ccaaaaagaa
     6781 tttattttaa gagattcttt tgctaaaaac caattccagc agctgatggc tgatattccg
     6841 gcagaggaga tcgaatgggt caaacaagta gtcgaactgg ctgaagataa actaaaagtt
     6901 gaattttcgc ctaatattta tctgacattg accgatcatc ttcattatgc gatcacacga
     6961 gcaaaagaaa atatccaact gcctaatcca ttattatttg aaacaaaaaa attttatccg
     7021 aaagaatatc aagcagcgaa agaggctctt ttgttcgtga aacaaaagac gggtgtggaa
     7081 ctttctgagg atgaagccgg ttttattgcc tttcattttg tgaatagcca acaaggaaac
     7141 gaagacatgc aagtgacgat gaccgctacc acaatggtaa gagatatact gagtatcatc
     7201 agtaaattct tcggcatcgt gttcgatgaa gaatctttga attatcagcg tatcattact
     7261 catctgcagt tttttgccca gcgttatctc aaaggggaag gatctgacga gcaagatgaa
     7321 tttctctacg cattagttca aggaaagtat ccaaaagcgt ttcattgtgt cgaacggatc
     7381 aatgagtatc ttcgacaaac gcaaaaacag gaaatgggga tcgcagagca gatttattta
     7441 acgattcata ttcaaagagt agtaagtgag aaaaaaagca tccaatcata aggattgtta
     7501 ctgtatggca ggcaaaacct ggattgaaaa aataggttca cgaatgtttg tgactgtttt
     7561 tttcaatctg ggtttttatt ttttaaataa aaaagaaaga aggaaaaaac aatggataat
     7621 caagcagttg gtcgtcgtgt atgggaagcg gtaggaggac aaaagaatgt caaaagttta
     7681 gtacattgtg ctacgcggtt gcgattcaga ttaaaagatg aatctttagc agatacgcaa
     7741 aagttaaaag aagacccgga cgtgatccaa gtcgtacaaa gtggcggtca gtatcaagta
     7801 gtgattggca gtaatgtagc agatgtttat caatcgatcg tggatgagga aggtcttgca
     7861 gtttcggagg atggaaaaga agagtccaaa aatattttga atcgtctgat cgatattatc
     7921 tctagtatat ttacaccttt tctaggggcg atggcagccg ccggggtatt gaaaggattc
     7981 ttatcattgg caacggtgat gggctggctg acagctgata gcggtactta tcaaatattg
     8041 tttgctgcag cagatggtgt ctttaccttc ttacctgtca tgcttgcttt tacagcagct
     8101 aaaaagttca aagccaacca gtttttagca gtagcaattg ctatggcact tgtgtatcca
     8161 gctatcacag cagttgctgg tgcgggagaa gctatcagtt tctttgggat cccagtgatt
     8221 ctttcaccat ctggttatac ttcttctgtc ataccaatca ttctagccgt atgggttcaa
     8281 agtaaattag agccattcgt aaaaaaagtg attccgcaat ttctgcaaat gattttagtt
     8341 ccgcttgtcg ttttagtagt gatggttcca ttaacatttc ttgctttagg accaatcggg
     8401 acggttgccg gtaatgcttt aggcggtctc ttcaactcga tttatggatt tagtccaatc
     8461 gtagcaggtc taatcatggg aagtttatgg caagtattcg tgatgttcgg catgcattgg
     8521 ggattcgtac cgatcatgtt tttgaatatc gaacaatatg ggtttgatgt attgatgcca
     8581 atgcttcttc cagccatctt ggcacaaggt ggggctgctt tagctgtagc attgcgaaca
     8641 aaagatacga aacttcgaac attagggatt tcttctacag tgacttcgtt atttggaatc
     8701 actgaaccaa ctgtgtatgg tgtgacatta cctttgaaga aaccattcat tgccgcttgt
     8761 atttctggag gtattggtgg agctattatc ggattttccg gagtaaaagc tttctccagc
     8821 agtttggtca gcttattgac gattccgact ttcattagta cggtggatgg tgtagagtca
     8881 aatgtgacag tagcagtaat agctacagga attgcttttg ttcttgcatt cgtaggtaca
     8941 ttgattttag gatttgacga acaaacacaa gacaatcaat tagaaaacaa acatgcgaat
     9001 gcaggagaac cgattacttc agcacggcat accttaaaaa gtcctttaac gggtaaagta
     9061 ctgccattga gtgaagtacc agaccaagtt ttttcttctg gcgtgatggg aaaaggtatc
     9121 gctattgatc cagaagtagg agaactagtt gcacctgcag acggagagat cacgactatt
     9181 tttccaactg gacatgctgt agggatcaca acgactgacg gagcggatat tttgattcat
     9241 atcgggatgg atacagtaga attaaatggg aatggctttg agatcttagt caaataaggg
     9301 gatctcgtcc aagcaggaga tttattgatt cgttttgaca tcgaagcaat tagagcagct
     9361 ggctatagcg taatcactcc agttgtcatt acgaatactg atgcatttgc cgatatactt
     9421 gaattagacc aaaaagagat tattgcaaac gaagatgttc tggcaattgt aaaataagga
     9481 ggaaatcagg atgcaatcac gtttttcaga aaaattttta tggggtggag caactgctgc
     9541 caatcaatta gaaggggctt atgatcgaga cggaaaaggg ctatccgttg cagatgctat
     9601 gcctggaggt aagcagcgct ttgcgattat cggcagtgaa gaatttgact ggacgattga
     9661 tcaagaaaaa tatatttacc cgaatcatcg aggaatcgat cattacgatc gtttcaaaga
     9721 agatcttgcc ttgtttgcgg aaatgggatt caaatgttat cgtttttcga ttgcctggac
     9781 acgaattttt ccaaatggag acgaaggtac tcctaacgaa gcgggattag aattttatga
     9841 tcagctgatt gatgagtgct taaaatatga tattgaacct gtgatcacga tttctcatta
     9901 cgaaatgcct cttcatttgg caaaagaata cggtggctgg aaaaatcgaa aactgatcga
     9961 cttctatgaa cgatttgctc agacagtatt agagagatac agcagtaaag tcaaatattg
    10021 gatgactttc aatgaaatca attcggcctt tcactttcca gctttaagtc aaggattagt
    10081 aaaaagcaat ggagcaggag agtaccaaaa tattttccaa gcatggcata atcaattcgt
    10141 tgcaagtagc aaagctgtaa aaatcggcca cgaattgcgt tcggatatcc aaatcggctg
    10201 catgattatc tatgcaacaa cttacagcat cgatgccaat ccagtcaatc aggcagcaac
    10261 aatgatccaa aaccaagaat tcaatttttt ctgcacggat gtccaagtac gtggagaata
    10321 ccctgcatac acagcgcgta cccataaaaa atatggagtc gatccggaaa aattagagca
    10381 aacggaaaaa gacttccagt tgctaaaaga atatccagtg gactacatcg gatttagtta
    10441 ttatatgtct acggcaatca atgagactga tccacaagca gcaacttcag aagggaactt
    10501 gctgggagga gtaaaaaatc cgtttcttga ggcaagtgaa tggggctggc agatcgatcc
    10561 agaagggctg agaatcgcat tgaatgaatt atacaatcgt taccagaaac cattattcat
    10621 tgttgaaaac ggtcttggag ccattgatca tatggaagag gatggcacga ttattgatga
    10681 ttatcggatc gattatttac gtcgtcatat cgaagcgatg gctaatgcag tagcagacgg
    10741 tgtagatttg atgggctata cgccttgggg atgtatggat cttgtcagtg catcaacagg
    10801 agaaatgagt aaacgttacg gctttatcta tgtggatcta gacgatgaag gaaatgggac
    10861 actgaatcga tcaaagaaaa aatcttttga ttggtataaa gaagtaattg cctcaaatgg
    10921 cgtaaacttg taattaaagc agctgaatga aaaagaacac acttctcaca tgtgatttgt
    10981 gttctttttt tattttttat gtgctattct tttagtaata gtaaagcaaa gggtgacgtt
    11041 atgaaaagga cacgtgtact atacttggaa gtcgcagata aaattaaaga agatattttc
    11101 tcaggaaaat atccagtcgg ttcgatgtta ccgacagaaa gtgaattaga agaactgttt
    11161 caagtcagca aaatcactgt ccgaaaggca atcgaactgc tggcatcaga tgaatatgtc
    11221 gcaaagaaaa gtggtcgtgg gacgacggtt ctaagcaatc gtccttacaa tcgattatcc
    11281 aaagcggcaa gctttacgca aatcttggag aactcccgat atgaagtccg gaaagaaacg
    11341 ctggatttgc aattggtcca tataaagaag gaagatcctt tgttttcctt tttcggtgag
    11401 caagctgttt gttttaagcg cttatattat ttgaacaatc agccttatat ttattttgtt
    11461 cattatcttc cacaagaaat gatgcaattc agcagagaag aatttggcaa agaatctttg
    11521 tatcgtttgc tgacgaaaaa aggatatact attgactcat tttctgatga tttctcggca
    11581 gtatttttaa cagaagaaga acaaggaatt ctgaaaacag acgcacagat cggcatcaaa
    11641 cggatacgga aatcgtctac gcaaattggg gaaatgattg aatattcgga ggcggtctac
    11701 catacggagc tgcatcctta ccatattgat tatgaaacat aaataaaaaa agaggttgtg
    11761 aaaaagctgc cctgcatcaa gtaataagaa cagccaaaca aaaatagctt ctcatatttt
    11821 tcgtttggtt aggcttatta ccgcagatac acggctgttt ctggattacc atttattctg
    11881 gataaggggg tgtggcacga ttttcagaag gaatctatga accaatcact gtattgattt
    11941 tagcttcgat ggtaacccca ttcggcttga cgatcgctta tttcttaggg aaaatcattc
    12001 gtaaaaatat attcaatcgt caagaaatcg atacattgaa gaccgctttt cctatgggga
    12061 tctgtcagat cacagaggga tgtttcccta tcgtattgaa cgacttggca cgcaatgtca
    12121 tagcaacggg agtcggtggg gcagtaggtg gcggattaag catgttttgg ggcgcagata
    12181 gccgtattcc ggcatcggga atgtttgcag tcgctacgat gaccagacca tgggcattca
    12241 ttggtgcatt gttagctggt tcttttgtca caggaataac cattttatta ttgaaaaaac
    12301 cagtagatcc gaatgcagaa atcatccaag aagaaaaaga ggaagaagac atatcttggg
    12361 atgatttgac aattagttag gagaggcaga atggattaca agaaaaaagt acaatagatg
    12421 taccttgagg caggaatggt atgaaagaat cagagttaga aagaatcgaa tatacagatt
    12481 ttggtttagg agcgatcgaa actgtccaga gcatcggcac cctccttgaa atgacgatga
    12541 aggaaaaatg agaacctttc gttgtcgaaa aggtctggta taccttatgt cgaaggaaag
    12601 gaaacaaaaa aactttcctg tactattcca aaaaggaaag aaagttatta tacagtaaaa
    12661 aaagaaatta ttcttcatgc tagagatcag tacacgttcg agcccaatat caaacactag
    12721 ttccaggcag gcgcaacagg tgcggttatc actgaatttt cttccagcag tgacgattca
    12781 agtgatattt ttacaaatcc agctatttat cgttaacaaa aagattttta ggataagctg
    12841 atgaacaaaa atgatcaaat tttgatcgtt tagttcgtcg gcttttttat ttagagaaac
    12901 atcatttatc agtgaaacat aacacgagaa caaaatgatt ttttcgttaa taaaagaaac
    12961 aagtatttgt tgtttaaaat aatacacgtt attggcatat aggacgaaat cccagtatct
    13021 tctataagta agtcatttca tatcgatgaa aacacgttat ttatttttgt tttatctata
    13081 aaatagctga aaaattattt tttctaaata ctatatatag tggtgtctat cctatatacc
    13141 actaaaaaac tacaaaatct agtcttttat tttaaaaaaa cacgtgtaga atgaagatag
    13201 acttttgagt tagggagtgt ttttttatga aatttactgt tttttgcggt tcccgttttg
    13261 gtaataaaga aagttacaaa tttattgcgc aaacgttagg aaaatacatg gcacaagaag
    13321 aaatcgaact ggtttacggt ggttcatact ccgggattat gggagtgatt agtaaaacag
    13381 tactggaaaa taatggaaaa gtcacaggta tctatccgaa tgggcttttt gaaactgaat
    13441 tgcctggaaa agatgttact acatttattc ctacagaaac gatcgatgaa agaaaggtat
    13501 tattatttga aaagggagat gctgtcctcg ttttccctgg aggtctaggt acattggagg
    13561 aattctcaca acttctttct tggatagcaa tcggtctgac gcctgacaag ccaatcggaa
    13621 tattgaatat tggcggttat tacagcggct tgcaacaatt attagaaact tttgcaaaag
    13681 aggattttat ggataaaaaa tggttggatc aagtaatctt ttccaacaat ccattggaac
    13741 tggtcaatga attacgagca gtatctatta aaaatcagtt attattgaag gaggcaaaat
    13801 aatgagtcaa atatatttag caggaccatt tttttcagag gagcaaatcg atcgagtaag
    13861 tcgtatcgaa aaagcgttgg aggaaaacaa aacagtcaca agtttctact ctccacgtca
    13921 tcaccaagaa agcaattatg aactattcag cgctggatgg gcgcaggaag tatacgaaaa
    13981 agatatggaa gaactgacaa atgcagaatt tgttgttgct atccttgact ttgagcatca
    14041 aacaatcgat ccaggaacgg cttacgaatt aggtgtggca actatgttga aaaaaccgat
    14101 gatcattgta caagaagaga cggtacctac gaacttgatg attacacaaa gtctccatac
    14161 gtatttaaaa tcagaccaag ctgtacgtga atatgatttt gaaacattgc ctgttgaaac
    14221 ttacgtaggc gagtacttat agaagaggaa gggttgtatg tcagtgattt tgatttcagg
    14281 aacgattggt gcaggtaaga gcagtttgac ggatatgctt gcaaaagaga ttgattctaa
    14341 accattttat gaaaatgtcg aagataacga agtattgccg ctcttttatt caaatcctga
    14401 gcagtacgct tttttattgc aaatcttctt tttgaacaag cgatttttag caatgaaaaa
    14461 tgctttagtg aacgatgaca atgtgctaga ccgatccatc tatgaagata gcctgttgtt
    14521 ccatctaaac gctgatttag gtcgggtaac ggatatcgaa gtacagcagt acgatagctt
    14581 attagatacg atgctaaacg agttagatga tgttgctcca aaaaaacggc ctgatttgat
    14641 ggtccatatc aaagtttctt tggacacgat gctagaacgt atcaaaaaac gtgggcgtga
    14701 ctatgaacag ctagaaagcg atgagacgtt atatacctat tatgaaacgt tgaacacccg
    14761 ctataatcaa tggtatgaag attttgatgt atgtcctaaa atacaagtgg atggggataa
    14821 attcgatttt gtagaaaatc cagaagatgc aaaaaaggtg atccatcaga tccaagaaaa
    14881 actagctgaa ttggaaggaa agcctgctgg aaaatatttt acggataacc agcatgcaaa
    14941 aggtcaagaa ttaggctaaa aataaaaaac caaacgacgg gaaagaaaaa ttactacgtg
    15001 atgtagaata agcctttcta tcgtttggtt attttatatt caaatatttg aaataaataa
    15061 atctttactc tttgatttgt aaactgaaat gatccactgt ttgttttact gatgaagctt
    15121 gatcaaagga agaagctgag tttgaagttc ataaaaatca gccatagtga gttttgcttg
    15181 gatttgttta tcttcttcta ctttttacat aattttgaac aggataagat tcaaatgttt
    15241 taaatgaatc tttgactagt ttgtgacagt tgtcgcatat gctcaagctt ccttggtcat
    15301 taaattctgt atggatattt ttgttgttat tacatgtaca ataatcagga ctatcgatca
    15361 tccatttcgt ctcctttaca atcatcttgc ttctttacta tagcagaaaa gaaggggaga
    15421 aacaaaagaa gatccaacag acggcactat agactgtaga tgttgccatc ctcatccttc
    15481 aaacggtaat taccgatttt ggtaaatgtg atggttgctt cttttttatt ttcttttcct
    15541 gaaataatat attcgtcatc tttggtagta atcttgatgt ctgtcagcac tgttttttct
    15601 tcagatgatt cagaagaatc agtttcttgt gtctgatacg tgcgtgtcca ttccattttt
    15661 ttatctgtaa atgtcaactg tcccgcggat aaatccatat ctttacgtac ttttagcgta
    15721 tatacactgt tagtgatatc tgctttttcc gcttttaaga cagaagaatc ggttgtttca
    15781 tccgaggatt ggtcagagcc gccaaacaaa ttacagccag tcaatgtcaa aagtcccgca
    15841 gacaaaataa atagtacgat taatttcttc aaaatacttc ccccatttga taggataaag
    15901 aaattatacc attggaaagt aaaacttcaa acggatggaa gagaaaaacg cacaaaacac
    15961 ccacaaatag agtaaatttt agaataaaat tttaaaaagg caaaaaattc gccattttag
    16021 gaaagcgttt taaaaaagtg ttgcaatctt ttaaaaaaat gctatattaa aaatgaaccg
    16081 gttcaaatac ttgtttaagt ataattaata gtgagaggaa gttgctctac tgaagacttt
    16141 agttatatgg atattgatga caagcaacac tatgcaaaga aagcggtcat cataatcgaa
    16201 caacaagaaa gagaaatcaa atgtaactgg caggagtgag aatgtatggc atcgatccgt
    16261 gacgtggcta agatggctgg tgtttcagtg ggaacagttt cccgttactt gaatggtcag
    16321 caactgaagg aaaagaacat gaaaaaaata gcagaagcga tttcagcgtt ggactataaa
    16381 gaaaacatca ttgctaaagg gttgaagaat aatcgaagct tctctattgg acttttgata
    16441 aacgggattt ccagccgttt tgggtcggaa gttgtctcag ggatcgaaag agtagctgaa
    16501 gaaaatgggt atagcttatt attaagcggg tttgccgatc agccagaaaa aattgaacaa
    16561 aaaattgaat acctgatgaa gcatgtgatc gatgggctga ttgtcttctt atccgaagaa
    16621 gagtggtctg gatttgaaat gctcacaaaa atgagtattc cagttatcgc attgaattgc
    16681 ccagaaggac cagcaggtat tgatacaata ttagtgaatg atcgagaaag tgtagcaaaa
    16741 gtgatcgctc atcatggtga acaaggacat aaaaaaatcg gatttattgc tgccactcaa
    16801 acagactacg tagcaagaga acgtttagca ggagcaaaag aagcggtaca aaatgatcca
    16861 tcgatccaat tagaggtctt tacaggagac tattctagaa aaagtggcta ttatggggca
    16921 aaagaattat tagaaaaagg agtaacagcg atttttgtca gcaattataa tatgtcgatc
    16981 ggtgctatcg aatgtttcaa tcaagaaggt atcagaatcg ggaaggatat tgcgtttagt
    17041 cattatgatt atttggataa aaacgatttg agcatccttc cgaaaatcac gattacacca
    17101 ccaacagata aaatcgggga acaagccgca gaacagctgt tgaaacgaat caaaaaagaa
    17161 gaggaagaaa atgaaaaagt aattgtgatg gaaaatatca tacatggaat caactaaaac
    17221 gaactaaaaa agaaaaagag gtgctttgat gaaaaaatat caatttccag caaatttttg
    17281 gtggggttct gcagcaagtg gcccacaaac agaaggaagg atttcaggtg atggaaaagg
    17341 agagaacatt tttgattatt ggtacaaaaa agaaccgcaa aagttctttg accaagtagg
    17401 acctgaaaaa acttcacaag tttatacgaa gtatcaagag gatgtacagt tgatgaaaca
    17461 gactgggcat aattcatttc gaacatctat ccaatggagc cgcttgatac ctgatgaatc
    17521 tggaacaatc aacgaagaag ccgttcgttt ctatcatcgt tattttgatg agttgattga
    17581 aaacggtatc gaagcctttg tgaatctgta tcatttcgat atgccgatgt atctgcaaga
    17641 aaaaggcgga tggttgaacc gagaaacggt tgaagcctat gaaaaatatg ccaaaagttg
    17701 ttttgaattg ttcggttcga aagtaaaaaa atggtttact cataatgaac caatcgttcc
    17761 agtcgaagca ggttatttat atcgatggca ttatccagaa gagtcagata tgaaaaaagc
    17821 gatccaagtt ggctatcatg aagcgttggc ctcagcgtta gccatcaaat cttaccatga
    17881 aatggagcaa ggtggtgtta ttggtatcat cttgaatctg acaccaagct atccaagaga
    17941 cgaatcaaac cctgaagatg ttaaagcagc gcaaatagca gatgcgttct tcaatcgttc
    18001 attccttgat ccagctgtaa aaggcgagtt tccaagtgaa ttgattgaga tagtaaaaga
    18061 acttgatctc atgcctgtga tccatccaga ggatttgacg gtcatcaaag aaaatacagt
    18121 agatctttta ggtatcaatt attaccagcc acgccgggta aaagcaaaag aaacaccaat
    18181 cgatactaaa aacggtccaa tgcctgagga ttatttcgac aattatgaaa tgcctggtcg
    18241 taaaatgaat ccttatcgcg gatgggagat ctatgaacaa ggaatctatg atattttgat
    18301 caatgtacga gatcattatg gaaatatcga ctgctttatt tctgaaaacg gtatgggtgt
    18361 agaaaacgaa gcacgattta tcaaagaaga tggcatgatc cacgatgatt atcggatcga
    18421 atttgtccaa tctcatctga aatatgtcca tcaagcaatt caagaaggtg ccaattgttt
    18481 aggctatcat atgtggacat gtatggataa ctggtcatgg accaatgcat ataagaatcg
    18541 atatggcttt atttcagtag acttagcaca agatggaaaa agaacaatca aaaaatcggg
    18601 tcactggttc aaagacgtat cagataaaaa cggatttgag gcttaagaaa gaatagttga
    18661 gagcaaatgc gtgaaattct ccattcttac cattttgtga aaaagaagtt caattattta
    18721 cgatcttttt caactttttt gtctttctat ttcatttttt gtatataata gaatagtcaa
    18781 aattaaatag gagtggagga aagcatgagg aaaaaaagac cgtttcttct cttttggtta
    18841 ttcggttttt tcttactgct tattttatcc gcagcatttt caggaaaaat gacagcagat
    18901 cttgctcaga aagaaacaga aaaaatcgta gcaagcaaag aatcagtaga taatttcaaa
    18961 aaagaattct caacttattg gtgggcggag tataaaataa ctgccaatta cgaaaaaggc
    19021 gaattggaca tctatttgcc aaaaggaaaa gaagaaaatc aatttattaa tatcgatgac
    19081 gtacttagtg cgatttctct cagacaatat caatttaaag acaagcattt gaccgttttg
    19141 cttttgactc atgatggcga caaagtagcc cgtttgaaaa atggaaaagt gatcaccgct
    19201 tattctgaaa aagtagacaa aaaagtggtc gccgatcgtt tagcggaatg gaaaaagaca
    19261 aatgaacaag tcaagaaaac aactgtctct tctcaaaaaa acgagtcgac aggaacaagt
    19321 ccttcagatt ctggcagtgg acaaacgata acgggaagtg aagaaaatca agcctcaatt
    19381 attacgaatg agtcatcaca aggacaatat caatcctatg gacaatgaaa aa
//
LOCUS       DAKBVG010000029        19419 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000029 DAKBVG010000000
VERSION     DAKBVG010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 19419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.029"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            52..3579
                     /locus_tag="OUD09_000776"
     CDS             52..3579
                     /locus_tag="OUD09_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HCT9692064.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3576..7298
                     /gene="addA"
                     /locus_tag="OUD09_000777"
     CDS             3576..7298
                     /gene="addA"
                     /locus_tag="OUD09_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HCT9692065.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7367..7708
                     /locus_tag="OUD09_000778"
     CDS             7367..7708
                     /locus_tag="OUD09_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCT9692066.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8017..9060
                     /gene="pheS"
                     /locus_tag="OUD09_000779"
     CDS             8017..9060
                     /gene="pheS"
                     /locus_tag="OUD09_000779"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCT9692067.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9065..11485
                     /locus_tag="OUD09_000780"
     CDS             9065..11485
                     /locus_tag="OUD09_000780"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCT9692068.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11655..12665)
                     /gene="trpS"
                     /locus_tag="OUD09_000781"
     CDS             complement(11655..12665)
                     /gene="trpS"
                     /locus_tag="OUD09_000781"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCT9692069.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12919..13656
                     /locus_tag="OUD09_000782"
     CDS             12919..13656
                     /locus_tag="OUD09_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCT9692070.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13670..14485
                     /locus_tag="OUD09_000783"
     CDS             13670..14485
                     /locus_tag="OUD09_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HCT9692071.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14498..15145
                     /locus_tag="OUD09_000784"
     CDS             14498..15145
                     /locus_tag="OUD09_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCT9692072.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15161..15817
                     /locus_tag="OUD09_000785"
     CDS             15161..15817
                     /locus_tag="OUD09_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCT9692073.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            15979..16800
                     /gene="racE"
                     /locus_tag="OUD09_000786"
     CDS             15979..16800
                     /gene="racE"
                     /locus_tag="OUD09_000786"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCT9692074.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16803..18158
                     /gene="rph"
                     /locus_tag="OUD09_000787"
     CDS             16803..18158
                     /gene="rph"
                     /locus_tag="OUD09_000787"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCT9692075.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18159..18677
                     /locus_tag="OUD09_000788"
     CDS             18159..18677
                     /locus_tag="OUD09_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCT9692076.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18784..19284
                     /locus_tag="OUD09_000789"
     CDS             18784..19284
                     /locus_tag="OUD09_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCT9692077.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6522 a         3340 c         4173 g         5384 t
ORIGIN      
        1 ttttttgata taataaagga gcaccgtatc atgtgttgaa ggaggaaaag catgtcttta
       61 caatttattc ttggaaacgg gaaaaaagat caccgtcaag tcttgatcga cgaagcatcc
      121 gcttggctcg accaggatgc aagtaaccaa gtatttttcc ttgttcccaa ctacagcaaa
      181 ttcgaacagg aacaagaaat tttagcggag atgcgaagaa gaagcggaaa aaaaactttc
      241 agcacaacaa acgttcaagt atttagtttt tatcgtctgg cttggtattt gctgcaacag
      301 acaactcttc ttacaggaaa tgaattatca gaatcaggat ctgcaatgat cttgcgacag
      361 attttagaaa aaaatgagga agaattaaca atctttcgcg gagaaatcaa taaaagaggg
      421 tttattcgtc aattacaaga actctacagt gaattgcgta caggaaatat tcaaccagaa
      481 gacctgatgc ttttatttgg tgattcacct aatgcagaag accagcaact taaaatgaaa
      541 gacttgaagc tgattttttc agcttatgac agcgaattga ctcaacgtgc actaacaaat
      601 gaagatgcac tatctatact agcaaattat atgcagacac aagatttcaa caacgtcaaa
      661 tttattgtca gtggtttttc tcgaatcaat ggtcaagagt atcaattact gcaggcaatg
      721 atggaaaagg ggcaattggt tgtcgattta ttgctggatc gtccttatac agcggatctt
      781 ccagaagtac ttacgctctt tcatgagact ggaaagcttt atttccgtct ttttcaaagt
      841 gcaagagaac ggaaagtccc tatattagtg gaccgatttg caccagagca gactcgtccg
      901 acagagcttc aactgctgca aacaatgtgg gaagagacat ctagccaaaa gaaagcggca
      961 gtacacaaaa tagggggaca agagcatctc catttgtggg aagctgaaaa tccaactgaa
     1021 gaagtccgac aattagctgt tgaaatcaga cgactagtca gtgaagaaaa ctaccgttat
     1081 cgtgatatcc aagtgctgac aaaaaatatg tctttatatg gaaatatcct tcggccgatc
     1141 tttcaagaaa tggcaattcc tttttatatc gatgaagaac aaatgatgga aaatcatccg
     1201 ctgatcgaat ggatcaacag tctgttcgct cttgaccgct attcttatcg gctaagtgat
     1261 attatgcgtt ttttcaaaac tgaattattt ctgtcgacgg aagcagaaac cgaacttgat
     1321 gaatggaaac aagagcgaga tgaattcagg aaaaaactgg acatcactga aaatgtagca
     1381 cttgcttaca attatcaagg aacttattgg actcgtgaaa aagactggca gtttgtggcg
     1441 tatgattttg aagcagaaca gattgaagac gtacagtatt tggaagaaca gtcaaatacg
     1501 attagacgat tatttcagaa agctgtacca gtatttttga aaaaaataaa gaaaagcaaa
     1561 acaggacaag aggccgcaaa tgttttttat caatttttga tccaaagtgg cgtacagcaa
     1621 caattattgt tttggcgtaa tcaggaagta gacagaggaa acttagaagc ggcaagaaac
     1681 catgagcaga catggggagc gctgattgat ctgttggatg aatatgtagt aatttatggt
     1741 gaatcggctt ttgactggtc actgttccaa gaaatcatta caagtggtct agaaaatcta
     1801 agttatggta aaatccctac cgcaatcgac caagtgcgaa tcaatcgttt ggaattagtt
     1861 cgaccaaatc aagctgccgt cacttttgca ttagggttga atgatcaagt gtttccagac
     1921 cgaaaagaga caaaaggatt gttgtccagt gaagaacgag aagtattaaa tcaggccttg
     1981 ccagatggac agtttttatt cgacccatca aaggaaagta tctcttttga gcctttccaa
     2041 gcctatcttg tttttctttc cggaacagat cgattatatc ttagttatgc acaaagctat
     2101 gacacagatg gttcgctgaa gatgtctcct tatttgcgta gaatcacaga atatttatct
     2161 gttcccgtcg agagaaaaga gcatttgacg atcgaaagcg accctaattg ttatgtaggg
     2221 acttacagaa gcggaatcaa tacagtcaat aggatttatc gattagcttt agatgaaaaa
     2281 aaacctgtac caaagcactg gcaaaagctc aaagaattag tactgaattc gtcatggcga
     2341 aattttgctt tacagatttt tgaaagtcag gaacatttaa atgttcctgt ttcgatgcca
     2401 gaaaaaatgg cggaagagct ttatggaaaa gagatttata cttcaatttc acgtatggag
     2461 aatttttata attgtgaata caaatacttc atgcaatttg gtttgaaatt gaaagaacga
     2521 actgtttatg gtctaactcc agcagcaact ggtgattttt atcatgaatc attggatcgt
     2581 ttcttcaagc tactgttttc aaaccaatta tccttagtga atatgtccga tgcgcagcgt
     2641 agagacttta ctgaaaaagt gctgcaggaa gtattcggag aacttcggtt tgagatattg
     2701 gatagttctg caagaatgaa ctatatccgc taccagttag gacagacgat tcaaaaagtg
     2761 acatgggctt tgcaaaaaca gagcaaaaat acaggcatgc atcctcttca gacagagatt
     2821 ctatttggtc agattgctgg agcaaaaggt atcacaggtc tggaattacc gttgagtaat
     2881 ggtggaaaac tccatgttcg agggaaaatc gatcgaatcg atgtagcttc tgaacaagaa
     2941 gatacttggc taagcgtcgt tgattacaaa tctagcggac gttcattcga tgtcacagaa
     3001 gcttattatg gcatggctat gcaattattg acgtatctgg acgtagcatt gatggatgcg
     3061 gtacgtttga ctggaaaggc ggctgttaaa ccagcaggat catattattt acatgttcat
     3121 aatcctgtat tgacggagac agatgatgta gagaagaata cattgaaaaa atttagttat
     3181 gatggcgttt ttgtcgatga tcccgctctt ttagaagtac tagatcattc actggaagca
     3241 aaagaacaat cactgatcta tccagtgaaa aaaaatgcga aagatgaata ccaaaaaggt
     3301 agtttcagta aagacaaatt tttcacggaa gaagaactgc aactattcat ggcgcataat
     3361 cgggaaaata tgcggttagc tggagatgcg attacttccg gagaaatcaa actaaatcca
     3421 gcttataaag ataaacagcg gattgcctgc gaattttgtc cattcagaag catctgtacg
     3481 tttgatgtga tgctgaaaga aaataattac caccgtctgg agaaaataga taaggaagaa
     3541 gcactgcgta gaatattgaa aaggagtgaa gaagagtgaa tcaaattcct ttaaaaccgc
     3601 aaaatgaacg attcacagat gatcagtggc aagcgatttt tgatcaagga gataatctat
     3661 tagtctctgc gtctgccgga tcaggtaaaa ctacagtgct tgttcgaaga gtgattgaaa
     3721 aactaaaaat gggttttgat atcgatgaat tattgattgt gacatttact gaagctgccg
     3781 caagagaaat gaaagaaaga attcaagagg cattacaaga atccgtcaat agcgaaagtg
     3841 accctgtacg tagacagcat tttactaagc agttagtttt attgcccaca gcgaatatct
     3901 caaccttaca tgctttttgc ctgacagtta ttcgacgtta ttattactta attgatattg
     3961 atccagtctt tcgcatgtta acagatgaaa cagaaacgat cttgatgaag gaggatgtgt
     4021 gggacgagct gagagaagca ctttatgcag aaaatgatga acgatttttc caattaacaa
     4081 tgaatttttc taatgatcgt tcagatgacg gcttgacgaa tctggtgttt tctctttatg
     4141 agtttgcccg tgccaaccct gacccccaaa aatggctaga acagttaagc gataattatc
     4201 gtttgcctga ggggctagcg aaatctcgat tatatcaaaa acaaatccgt cctttagttt
     4261 tggcagatat ataccaatgt gtacagttat atgaacagat gactcagtta gcacaaggtg
     4321 aaggattaga aaaaatgcat gagcaagtag ctggagaaca acagcagatc aagaatatct
     4381 atgaagcgtt ctcgcaagat cggttagaag aagcttatgc tggtttggaa caactaactt
     4441 tttcaacttt taagagcagt cggaaagcag aattgaaaga aatttccaat gaagtcaagg
     4501 gcatgaggga taaagcaaaa aaactgattc agcagataag taaaagttat tttcctgttt
     4561 ccccaagcca aatggaagaa ttaacggaca aagcactacc gttagtcgaa gaaatgacaa
     4621 aagtcacgca aagcttcatg gacggtttca gtatgcgtaa acgggaaaaa ggtgtgctgg
     4681 atttcaatga tctggaacat ttagcgttgc agattctcac agaaaaaacg aaagatgtat
     4741 ggctgccaag tgaagcttca aaacattatc gaaaaaaatt caaagaagta atggtagatg
     4801 aatatcaaga cgtgaaccaa ttacaagaag ccattttata ctggctaaga gaacccgatg
     4861 atacaaaagg aaatatgttc atggttgggg atgtcaaaca atccatctat tctttccgtt
     4921 tagcagatcc tagtttattt atcgggaaat acgagaattt ctcaaagaaa gaaggcggac
     4981 gtcgaatcgt tttggctgag aacttccgct ctagaaaaga agtcctttct tttacgaatc
     5041 ttatttttga acaactaatg gatccagcag tgggtcaaat caattatgac gaagcagcca
     5101 aactgatcca aggattttca gactttccag agaatgaaca gtttgagccg gaaatcatga
     5161 tctatgaaaa agaacaggaa gaatcagaga ttgagattcc gacggatgat atactggaag
     5221 acaaaacaga aggcgaactt tttatgacag gactaaaaat tcgtcaatta attgattcct
     5281 cctttatgat ttatgataaa aaatcaaaaa aaagtcgtcc gatagagtac aaagacattg
     5341 ttctcttgac acctacaaag aagaacaact taacgatttt agagattttc aaaacattgg
     5401 atattccatt agagatgaat gatgctcaaa actattttca agcgacagaa attcgcacga
     5461 tgatctcgct gctacaatta atcgataatc cctatcaaga tattccgctt gcggctgtat
     5521 tgcgctcacc gatcgtgggt ctgattgaac cagaattagc cagcattcga ttagctaacc
     5581 gtgcacatac ttactatgat gctgtgcttg cttatcaagc atcaaacgaa gatgaattag
     5641 cagctaagct ggaacatttt ggcaagcaac tagagcattg gcgagaactt gctcgccgtt
     5701 cttcgatcac tgacttgtta tgggatatct attatgagac gggatatcta gagtatgtgg
     5761 tcggtctgcc agcaggggca cagagacaag ccaatcttta tgcattggtt gatcgtgcca
     5821 aagcttacga gcaaagttct ttcagaggat tataccagtt cgtgcgtttc attgaaaaaa
     5881 tgcaagaaaa agataaagac ttagcagaac cagtcatttc aatagaagat aatgctgttc
     5941 gcgttatgac gatccatgca agtaaaggct tggaattccc agttgtattt ttgttagata
     6001 tgacaaagga gttcaactta caagacttgc gcaatcggta tgcttttgaa gaaaaacttg
     6061 gagcaggaat tcgctatatg gatcctgaaa cacgagtgtt atatgatacg ttaccgtttc
     6121 aagccatcaa attggcaaag caaaacaaat tgttgtcgga agaaatgcgt aaattgtatg
     6181 taggattgac tcgggcagaa caaaagcttt tcattgtcgg ttcctataaa aataaagagc
     6241 aaatgatcca aacgtggtct gaagcagctg atcatgagga gttagttttt gatcctgcgc
     6301 tacgtttgaa gggcagaagc agcttgatga attggattgg ttacggattg atccgtcatc
     6361 ctgaaatgca gaaatactta gaggaagaaa tttcgacttc tcttcttcaa cattcaaatg
     6421 cacagttttc tatttcatgg atgaatcagc aatcgatcat tgaacaaaga cagttgcttg
     6481 ctgaaaaaga actggttaac ttagatcagc agatgaaaga agatgaaact ttattagcag
     6541 attcgttaca aaaacgtctg gcatatgaat atccttatca agcttcttca caaaccacaa
     6601 gttatcaatc tgtatctgaa atcaaacgat tattcgaaga tccagatgat acacaagaat
     6661 cacgtttgac tctggaaagc agtcagaaca aagcagcatc ccgtcaattt cgttatacgc
     6721 aagaacaact agcagaacca aaatttttgc aaaaagatcg gcaagtgtca gcagctgctg
     6781 ttggaactgc tacgcatgct ttgttacaat tactgccatt agagatgcca actacagaga
     6841 gtatccatca aaaattacag gaattggtaa ggaaacggtt agtagacgaa aaagtcgcga
     6901 aaaaagtaga tgtttcttct attatatggt tctttcaaac agaacttggt caacagctga
     6961 ttgccaacaa agaaaacgta aaacgagaac aacctttttc catgctgctt cctgctgacg
     7021 aagtatttca agactatcca aatcaagaag atgaactatt aatccacgga atcgtcgatg
     7081 gatacctaga agaaaaagat catctcaata tctatgattt taaaacagat tttattttac
     7141 atccggatga tccagcagaa atagatgcaa tcgtccaaaa atatcaagga cagctaagac
     7201 tttaccaaca agcaatgtca gaagctttga ataagccagt agaaaatgtt ttcctaattt
     7261 tgcttcgagt gaaacaaatt ataaacatta acaaataatt ataattgagc aaacttgact
     7321 tatgttttgt aaggattatg ctatactctg ggaagaggtg agtatgatgg agctaacaca
     7381 gacagaattc gttttatgcc ctaaatttga aaaaacattc tctattttag gaaaaaaatg
     7441 gaacggattg attatcgatg tactactcga aaacggtccg caacgctttg ttgagcttgc
     7501 ggcaaaaatc ccgatggtca gtgatcgagt attagtcgaa cgcttgaagg aattagaaaa
     7561 agaaggaata gtcactcgaa actttgcctg tagcgaaaag agccgttcag aatatctctt
     7621 gactgaaaaa ggagcagact tgcaaaaagc gatgcgtcaa atacaagttt ggagcgaaaa
     7681 atggatcaca gaggaagaat gcggttgatt ttgtaaaaaa aatcttgtaa actatagtcc
     7741 atacgaataa gtaaagaacg atgacgaaaa agagtagttt ggctaaacca gttcagggag
     7801 atctgtcgca gactgaaagc agatcctgtg tttgaaaaat gaagttcact ttccgagttc
     7861 gtctgataaa atcagaccgg caattgtcgt taacgaagta agagtgcggt gtaagtcatt
     7921 tatccgcaaa ttaggatggt aacgcgaaga cctcgttcct taacaggaat gaggtctttt
     7981 tgttttgtat ttggaattta aaggaggaac aagcacatgt ctctacaagc acaattagag
     8041 agcttgaaag aagcaacgct cgtcaaaatc acagcggcac aagatttaaa tgaattaaat
     8101 caaatacgtg ttgaaacgtt aggaaaaaaa ggaccgatca cagaagtatt acgtgggatg
     8161 aaagatctaa gtccagaaga gcgaccaaaa gtcggaagtt tcgcaaatga gatccgagat
     8221 cttttgacag aagagatcga gcgaaaaaaa caagtattag aagaagaagc gttaaatgca
     8281 gctttggcaa aagaaacaat agacgtgaca ctacctggta gaaaagtaaa acatggtaca
     8341 cgtcatatcg tgacacaaat catggaagaa attgaagata tttttgtggg tatgggctat
     8401 cagatcgtag aaggatatga agtagaatct gatcattaca atttcgaacg gatgaatctg
     8461 ccaaaagatc atccagcaag ggacatgcaa gatactttct atatttcaga cgagatattg
     8521 attcggacac atacttcacc agtccaagca cggacaatgg aaaaacatga tttctctaag
     8581 ggtgctctac gaatgatctc gccagggaaa gtcttccgca gagatacaga tgatgcgacc
     8641 cacagtcatc agttccatca aattgaaggg ctagttgttg ataaaaacat cacaatgggc
     8701 gaccttaaag gaacattaga agtcgtaatg aaaaaaatgt ttggggaaga tcgtgaaatt
     8761 cgtttgcgtc caagttattt cccatttacg gaaccatcag tagaagtaga tgtcagctgt
     8821 ttcaaatgtg gtggtgccgg ttgtaacgta tgtaaataca ctggatggat cgaaatttta
     8881 ggagctggca tggtacaccc gaatgtgttg aagatgtcag gaatcgatcc agaagaatat
     8941 tcaggctttg ctttcggcct tggaccagac cgcgtagcta tgttgcggta tggagtgaat
     9001 gatatccgta atttctatca aaatgactta cgtttcttaa gtcagttcaa gggggagtaa
     9061 gaaaatgtta gtttcatata aatggctcaa tcgttatgta gatttatcga atgtaacacc
     9121 aaaagaatta gcggataaga tgtctgtgac aggaatcgaa gtagagggag taacggttcc
     9181 agaagaaggc ttgaaaaaaa tcgttgtcgg tgaagtcaag gaatgcgtac cacatccaga
     9241 ttctgatcac ctttctattt gtcaagtcga tattggcgaa gacgaactgt ctcaaatcgt
     9301 ttgtggagcg cctaatgtga aggttggaat caaagtgatt gttgcacttc ctaactcacg
     9361 tatcgctggt aatgtaaaaa tcaaaaaagg aaaaatgcgt ggccaagtat caaatggcat
     9421 gatttgctca ttacaagaaa ttggctattc agatagtgtt ataccgaaag aatacgctga
     9481 aggtatctat tatatgccac aagaagctgt aaatggcgat ccagtttttc cttatttgga
     9541 tatggatgat tcgattattg aattatctat cacaccgaat cgtgcggatg cattgtcaat
     9601 gcgtggcgta gcttatgaag ttggggcaat ttatcgtcag actcctacat tcgatgacga
     9661 aaagctggta gaatcctcac gtcctgctag cgataaaatt caagtggaag ttgaagataa
     9721 agaagcagtt cctgcttatc aaatcagaat cattgaagga gtaagaatcc agccaagtcc
     9781 tcaatggctg caaaatcttt tgatgaacga aggcattcgt ccaatcaaca atgtagtaga
     9841 tgtgacgaac tatattttgc ttttattcgg acaaccacta catgcatttg actacgacaa
     9901 attgaaatca aaagagatct ttgtccgccg tgcaaaagaa aatgaaacat tagtaacact
     9961 agatggagaa tctcgggaat tgacaactga caatctcgtc attactaacg gcaaagaacc
    10021 agttgcttta gctggtgtca tgggagggtt agactcagag atcacgaatg aaacaacaac
    10081 agttgcacta gaatctgctt tatttgatcc aatttcgatt cgtcgtacag caaaacaatt
    10141 taatttgcgt agtgaatcct ctgcacgttt tgaaaaagga atcaaccatg cgactgttgg
    10201 agaagccagt gaggttgctg cagcaatgat cgtttctctt gcgggtgggg aagttctaca
    10261 aggcgcggtc aaaggctcag agatcaaagc agaagacgta tcagtgaaga tttcacttga
    10321 acgcatcaat caatatttag gaacatctct tgtcgttaaa gaagtaaatg agatttttga
    10381 agcacttgga tttagctatg aagaaaagga gtatgtttat tccgtagtag taccaccaag
    10441 acgctgggat atccaaatcg aagcagatat tatcgaagaa gttgctcgta tttatggata
    10501 cgaccagttg ccatctacac ttccaagtgg agaaacggtt gctggaagct tgtcaaaaga
    10561 acaagaaact actcgtaaaa ttcgtacaat ctttgaagga agcggattga gcgaagcaat
    10621 cagttatgca ttgacaacag aagaaaaatc aagacagttt gttataaatg aaagtaatat
    10681 tacacgtttg gactggccaa tgagcgaaga acgttctgta ttacgaatga atttgatcag
    10741 cggactatta gatgatatca gttataacac tgcccgaaaa aatacagaga ttgctttata
    10801 tgaaattggt cgggtgttct tccaagaaaa tgatccacgt acacatctgc cgcaagaagt
    10861 aaaacatgca gcaatcgcta tttctggtat ttgggaagaa aaagattggc agacgaaagc
    10921 tcaaaatgta gactttttca ccatcaaggg tcttttagaa aacttatttg aacaactagg
    10981 catcactgaa gatgtccatt atcaaacagt gacagaaatg aaggaaatgc atcctggaag
    11041 aactgctgcg atttaccttg gcgaaaaatt tattggtttt gtcggtcaag tgcatccaac
    11101 gattacaaaa agttatgata ttccagaaac atatgttgct gagtttgatt tatctgctat
    11161 agtggaagct gctcaaaaag ggatcgtttt cgaagcagtg acaaaattcc cagcagtcag
    11221 ccgggacatc gctttgcttg taaaagaaac tgtgacaaat caggaattaa cagaagttat
    11281 ccgttctgct gccggaagat ttttaacaga tatccaatta tttgatgtct atcaaggaga
    11341 aaacattgaa aaaggacata aatccatggc gtatagcctg acattcgtca acccagaagc
    11401 aacattgaca gatgaagaaa tcaataaagc aatggaaaaa gtcgaaaaag cgttggtaga
    11461 aaacctagag gcaagtattc gctaagaagg aaaatatagt aagtaacgat caagtagctt
    11521 ttgaagacca tacatttgaa aaaaagatgt gacacaactt cgtcacatct ctttttttga
    11581 ttttaaaaaa ggaatagcac agagacgaga taaaaaaaca ggcctttaca gcctgctttt
    11641 ttagagtaaa tgaattaatg gaagtaattg attcccattg ctgattttac ttcagaaagt
    11701 gtttgtgcag ctactttagc agcttcttca ctacctttac gtaagatttc cataacagct
    11761 gaaggatctt tttccaattc ttctcggcgc gcacggatcg gtgcaaattc agcttccaat
    11821 acatcaatga ggtatcgttt gatctttaca tcaccaagtc cgccgcggcg gtaatgagct
    11881 ttcatttctt caattgcttc tttatctgtt ccgaatacat ccaagtaggt gaagaccata
    11941 ttcccttcta cttgtccagg atcttgtaca tggatgtggt ttggatcagt atacatactc
    12001 atgacttttt tctgtaagac atcggcagga tcggagatat aaataccgtt tcctaacgat
    12061 ttactcattt tgccatttcc atcgattcca ggtaatctac ccattccttt tggtgggaat
    12121 acgccttttg gttccaccaa gacttcacca tatgtatgat tgaagctttg tacgatttcc
    12181 tgtgtctgct ctagcattgg tttttgatcc tcaccaacag gtacaagatt cgctttgaat
    12241 gcagtgatat ctgctgcttg agaaacagga tagataaaga atcctgtagg cacgctttca
    12301 ccgaattttt tttgttcgat ttctgttttg acagtcggat tgcgccgaac gcgtcctacg
    12361 ctgactaagt tcaaataata catggttaat tcagctaatt ccggaatttg tgattggata
    12421 aataaagtag attttgcagg atctaatccc acggctaaat agtctaaagc aacttgcaag
    12481 acatttgatg aaaccttctc aggattttta gcattatctg tcagtgcttg catgtcagca
    12541 atcatgacaa acagttggtt gtcttcatct gcctgcatag ccactcgggt ttttagagaa
    12601 ccgacgtaat gccctaaatg cagcttccca gttggtcgat ctcccgttaa aataatattt
    12661 ttcaaaaaag acacctcttt acgatttgta agtctgagca cttaatatct taccgttttt
    12721 cctgaaaaga aacaacagtt cctaaggaaa aaacaaggaa agaggataaa ataactaata
    12781 gattagaaat ggaagaaata tttatgggag aataaacgtt ctaagaaact ttacaaatta
    12841 ctaaactatc agatataatg tattttatta tacatcttta aaagaaatct tactaaataa
    12901 taatgggagt gtaattctat ggcaatgatt aaatttgatc acgtggataa atattatggc
    12961 aagttccacg cattaaaaaa tatcaatctc gaattcgaga aaggagaagt tgtcgttgtc
    13021 attggtcctt ctggttcagg aaagagcacg atgcttcgct gtatcaatgg attagagacg
    13081 atctcttcag gtaagctgct gattaatgat actgacttgc atgataaaaa gacaaaactg
    13141 acagaagtcc gaaaaaacat cggcatggtt ttccaacatt tcaatttgta cccaaataaa
    13201 actgttctgg aaaacatcaa attagcacca atcaaagtgt tgaagcaaga ccaggcaact
    13261 gcagtcaaaa acgcggagaa atttttgaaa actgtcaata tgttagataa gaaagattct
    13321 tatccttcca tgttatccgg gggacaacag caacgggtgg caatcgccag aggtttagcg
    13381 atgaatcctg agatgctgct gtttgatgaa cctacttctg ctttggatcc ggaaatgatt
    13441 ggtgatgtat tagacgttat gaaaaaattg gctcatgacg gtatgtcaat gattgtcgtg
    13501 acacatgaaa tgggctttgc caaagaagtg gcggatcgtg ttatcttcat ggctgatgga
    13561 gaagtattgg aagacagcag aaatgtgaga gactttttcg aaaatccaaa tgaagtccga
    13621 gcacagcaat ttatcagtaa agttatcaat cattgatagg aggaaaaaga tgcgcaggtt
    13681 aaaaaaaata cgcctgattt tccttttagg cattgctttt cttgcactag ccggatgtaa
    13741 aggaaatggg ttggcaaatg aagatatttt agagcgaagc aaatctacca atgaaatcat
    13801 ctggggtgtc aagtatgata ctcgattatt tggaatgatg gatattgaga gcagaactgt
    13861 acaaggattt gatgtagata ttgcaaaagc aatcactaaa aagatccttg gtgacaatgg
    13921 aaaaacagaa tttgttgaag tcacttctaa aactcggatt ccattattga aaaacggaaa
    13981 tatcgatgca attattgcaa cgatgacgat cacagatgaa cgaaaaaaac aggttgactt
    14041 ttcagatgtc tatttcgatg ctggccaagc tttgttggta aagaaaggaa gccaaatcaa
    14101 aagtgtagat gatttgaatg cttctacaac ggttctagca gtcaaaggtt ctacatcggc
    14161 tgcgaatatc cgacagcatg caccagatgc aaaaatttta gaattagaaa actatgcaga
    14221 agcatttact gctttacaat ctggtcaggg agacgcgatg actacggata atgcgatttt
    14281 gttagggatt gcagatgaaa atccagagta tgagttagtt ggtggaacgt ttactaatga
    14341 gccttacggg attgcgatca acaaagggca agaaaacttc ttgaaagctg ttaaccaagc
    14401 gctagaggaa atgcatgcag atggcacata cgataaaatc tatcaaaaat ggtttccaaa
    14461 tgaaactgaa ggaaaagtag agtaaggggg agaggcaatg agcgaattga tacaaactta
    14521 tggaccaatc tttgtcgacg ggttcaagat cacattgttt tcgagtgtct tagcacttgt
    14581 gtttagtttg attatcggaa cgttaatggc aatcttccaa ttatcaaaga ataaagtagt
    14641 gagcgcctta gcaaaggcat atgtagaatt ttttagaaac attccattat taatcatcgt
    14701 gatgtttttt tatgttgtcg taccgattta ttggtttaat ctagatggat tccaagcagg
    14761 tacgattgga ttaacgatct atacatctgc ttttattgct gaaacagtac gttcagggat
    14821 tcaaaccgta ccaaaaggtc aaacagaagc aggactatct tctggtttta cctattcaga
    14881 aatcatgcgt tatattgttt taccgcaagc attcaaaatc gttatcccgc cattaggcaa
    14941 ccaatttatc aacctcgtaa aaaactcatc tattttagca atcgtagctg gtttagattt
    15001 gatgtatcaa ggagacttga tcgctagtga aacattcaat accttcgata cgtatatcat
    15061 cgttgggtta ttttacttag ttatcacgct accgttatct tacttaatgg tttatcttga
    15121 gaaaaaatgg gcggttcgtt catagaagga ggggaaatac atggatttta gcggcgcttt
    15181 ttcatggatg aatatccgtt ttttattgga aggattaaag gttactgttg aagtttcatt
    15241 gttttcgatc atttttagtt tcttgatcgg tggtctgaca ggtgtgattc gttttgcgaa
    15301 tatccctttc ttttccaaaa tccttggtgt agtgattgat attatacgga atcttccatt
    15361 attattgatc attttcttca cttactttgc cttacctcaa atcggcattc agatgaacat
    15421 cttctggtcc gctgtcgcag cattaacgat ttttgagtca gctatgttgt cggaaatttt
    15481 ccgagctgga ctcaatgctg ttccaaaagg acagatggaa gcaggattat caacgggact
    15541 gacttatgta gaatcgatgc gaacgatcgt gttaccgcaa gcatttaaat caatgatccc
    15601 ggcaattgtg agccaattga tttcactgat caaagatact tcattagcag taatcatttc
    15661 tttacctgaa ctttctcatc aggcacgtat tatttatgga cagaacacca attatgtgct
    15721 tccgatgttc atcatcatga cacttatgta cttcgtggta tgttacgcgc tttctctgct
    15781 ttccggttac ttggaaaaga gacggtacag ttattaatag atgattcttt tagcggttgt
    15841 ggcaaactaa agtcacaacc gcttttcctc tttccattcg aaaaaaagag atttcgattg
    15901 ttttaagcaa gtaagagcac ttgaaaattc acgctaaaaa gtacacaata gggaggatag
    15961 aatttattga aaaaaaggat gatacgattg acagataatc gccctatcgg atttattgat
    16021 tcaggtgtcg gcggcttgac tgtagtaaaa gaagccctga aacaattacc gaatgaaaat
    16081 attttatttg taggagacac agcacgctgc ccatatggcc ctagacccgc ggaacaggta
    16141 atccagtata cttgggaaat gacggattat ctggtggagc aaggaatcaa gatgctggtg
    16201 atcgcctgca ataccgcaac tgcggtggct ttagaagaaa tcaaagctgc tctttctatt
    16261 ccagtcatcg gtgtgatcct tcccggtact agagcggcag taaaaaaaac acaaaataaa
    16321 caagttggca ttatcggtac gattggtacg gtaaaaagtc aagcttatga aaaagcactg
    16381 aaagagaaag taccagaatt gactgtgaca agtcttgctt gtcccaaatt tgtttcagtt
    16441 gtcgaaagta atgaatacca ttcatcggtg gcgaaaaaaa ttgtggcaga aacattagct
    16501 cctttaacca ctaaaaaaat cgatacattg attttgggat gcacccatta tccattatta
    16561 cgccccatca ttcaaaatgt aatgggagaa aatgttcaac tgatcgattc tggagcagaa
    16621 acagtaggtg aagtatctat gctgttagat tatttcaatt tgagcaattc accgcaaaat
    16681 ggtcggacat tatgccagtt ttatacaact ggctctgcca aacttttcga ggaaatagct
    16741 gaagactggc ttggaatcgg acacttaaat gtagaacata tcgaattggg aggaaaataa
    16801 ggatgcgtca tgacgggaga aaagcccaag agcttcgcaa actggagctt caaacaaatg
    16861 ttttcaaata tccagaagga tcggtagtga tccgttttgg tgatacaaca gttatttgtt
    16921 ctgcaacaat agaaaattct gttccccctt ttttgagaga aacaggaaca ggatgggtga
    16981 cagcagaata tagtatgctg cctcgagcaa cgagtacgag aaatcgtaga gaaagcagca
    17041 aagggaaact ttctggtaga acaatggaaa tccagcgtct tattggtcgt tccttgcgtg
    17101 cagtagtaga cttggaaaaa ttaggagaac gtagtatcat cgtagactgc gatgtgatcc
    17161 aagcggatgg cggtactaga acagcaagta tcactggggc atttgtggca ttgaaattag
    17221 ccattgaaaa acttttaaga gaaaaagaat tgtcagaaga tccaataaaa gaacatcttg
    17281 ctgcagttag cgtaggaatc ttaccagatg ggacttgcgt cacagatttg gattaccaag
    17341 aagattccgc agcacttgta gacatgaatc ttgtaatgac agaatctgga aaattcgtgg
    17401 agattcaagg aactggagaa gaagcaacgt ttgatggtga acagttgaat gaaatgctct
    17461 tttttggaaa aaacgcgatt gaagatttga tcaaagagca aaaacatgct ttgctaacag
    17521 agtttgctca aaatgatgag cggatagaag aaacaaaaac aatcatcatt gcgacaagaa
    17581 atccaggaaa agcagaagag tttcgaaata tgttcaaaga agcgggatat catgtaaaaa
    17641 cattacttga ctatcctgaa ttgccagatg tggaagaaac tggcagcaca tttgaagaaa
    17701 atgcccgatt aaaagcagaa acgattgcac aattgcttga tcagccagtc ttagcagatg
    17761 attcaggttt gaaagtggat gctttaggcg gtatgccagg aatctattct gcacgttttg
    17821 caggggaaca aaaaagtgat gcggggaata acgccaaatt attatatgaa ttgacagatg
    17881 tgccggatga aaaaagaaca gcacaattcc attgtacgtt agtattcgcg gcacccaaaa
    17941 aagacagcct tgtagtagaa gcggaatggc ccggtcgagt agcccgtata ccatctggcg
    18001 aaaatggctt tgggtatgat cctttattta tcccagaagg taagaaacaa acagcagcag
    18061 aattatcgag cgaagaaaaa aacaagatca gtcatcgggc tcaagcaatg aaaaaattga
    18121 gtgccgagtg gaaacaatgg ctggaaggag agagatagat gcggtattta gtagtaagtg
    18181 ataatcatgg agacagagat atcctcgttg acttagtaga tcgttatcgt tctgaagtgg
    18241 atatcttttt ccattgtggt gattcggaat tagaagcttc tgacccatta tgggatacat
    18301 tccaagtggt tcaaggtaat tgtgattatg gaccaggatt cgaacagaaa aaagtgatcc
    18361 aaactggtca ggatacagta ttcatgactc acggtcattt atcgaatgtc cgatttggtt
    18421 tgacacagtt agcaatcgaa gctcgagctg caactgccga tatggtgttt tttggtcata
    18481 cccatcaaat aggctgtgag atggatcaga aaatcctgtt tttgaatcct ggtagcattt
    18541 ctcagcctag aggcccgatc caaatcccat catatgctct tatcgaaagt ttagatgacc
    18601 aaatttctgt tcaatactat aaccgtgccc atcaggcagt tgaagagttg gccttttctt
    18661 tttcgagaga gcgctgaata aaaaaactct aatttttaat aaaaataagc tttattttat
    18721 gaattttgca tccggtttcg gtaaaatagt agtgttgaat gaaatagaaa tggaggccat
    18781 cagatgattg gacctatagt aagagaaatg ctgctggaaa atcaggaaac gtttttgatt
    18841 cctgcagaaa atgtggcaaa tgttatgtat catcatccat tgtcccatgg tttacttgtg
    18901 ttatcgaagg tcggttattc caaaattcct gtattaggaa aagatgatca ctttgtagga
    18961 ctagtaagtt tatcagatgt ggtcaataaa atgattgact tgcaaacaat cagcatggac
    19021 ccgttagaag gactaacggt tgctgatgtg acagaaacag atgttccgac aattgatgaa
    19081 aattgggaac tagaagaagt actccatttg ttagtcgatt cgcctttttt accggttgtc
    19141 acagaagatc ggattttcaa aggaatcatt acaagaaaag agctgcttaa agccgtaaac
    19201 ttcatggtgc atgaattaga acgtcgtaat atattgtctc cgaaaatcga agaattaaaa
    19261 gaacaaatcg atatcgtgag ctaaagaacg agaaactaaa agataatcaa aaaataagta
    19321 gaaaaaacag cgtaccagtt ttgactttat cttgaaaaag aggctgggac aaaagcgttt
    19381 aactccaaga aataagaagg aattcacgaa aattgcttt
//
LOCUS       DAKBVG010000030        19076 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000030 DAKBVG010000000
VERSION     DAKBVG010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 19076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19076
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.030"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(39..764)
                     /locus_tag="OUD09_000790"
     CDS             complement(39..764)
                     /locus_tag="OUD09_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HCT9692078.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(1125..1195)
                     /locus_tag="OUD09_000791"
     tRNA            complement(1125..1195)
                     /locus_tag="OUD09_000791"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1161..1163),aa:Gly,seq:ccc)
     gene            1312..2064
                     /locus_tag="OUD09_000792"
     CDS             1312..2064
                     /locus_tag="OUD09_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HCT9692079.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            2080..2559
                     /locus_tag="OUD09_000793"
     CDS             2080..2559
                     /locus_tag="OUD09_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HCT9692080.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(2810..4390)
                     /locus_tag="OUD09_000794"
     CDS             complement(2810..4390)
                     /locus_tag="OUD09_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HCT9692081.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(4383..5126)
                     /locus_tag="OUD09_000795"
     CDS             complement(4383..5126)
                     /locus_tag="OUD09_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT9692082.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(5302..6078)
                     /locus_tag="OUD09_000796"
     CDS             complement(5302..6078)
                     /locus_tag="OUD09_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCT9692083.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(6326..7162)
                     /locus_tag="OUD09_000797"
     CDS             complement(6326..7162)
                     /locus_tag="OUD09_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HCT9692084.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(7173..8102)
                     /locus_tag="OUD09_000798"
     CDS             complement(7173..8102)
                     /locus_tag="OUD09_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCT9692085.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            8294..8647
                     /locus_tag="OUD09_000799"
     CDS             8294..8647
                     /locus_tag="OUD09_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCT9692086.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(8721..9377)
                     /locus_tag="OUD09_000800"
     CDS             complement(8721..9377)
                     /locus_tag="OUD09_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HCT9692087.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            9603..11537
                     /locus_tag="OUD09_000801"
     CDS             9603..11537
                     /locus_tag="OUD09_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCT9692088.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(11623..12846)
                     /locus_tag="OUD09_000802"
     CDS             complement(11623..12846)
                     /locus_tag="OUD09_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HCT9692089.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(12840..14738)
                     /locus_tag="OUD09_000803"
     CDS             complement(12840..14738)
                     /locus_tag="OUD09_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HCT9692090.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(14732..15850)
                     /locus_tag="OUD09_000804"
     CDS             complement(14732..15850)
                     /locus_tag="OUD09_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HCT9692091.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(15933..16766)
                     /locus_tag="OUD09_000805"
     CDS             complement(15933..16766)
                     /locus_tag="OUD09_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCT9692092.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(16759..17676)
                     /locus_tag="OUD09_000806"
     CDS             complement(16759..17676)
                     /locus_tag="OUD09_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCT9692093.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(17699..17878)
                     /locus_tag="OUD09_000807"
     CDS             complement(17699..17878)
                     /locus_tag="OUD09_000807"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9692094.1"
                     /translation="MNKINENTSNTVLPATYSDATSYLTTAAYNYWQTGDFENSYLKE
                     AENFAHKYGVKLTGD"
     gene            complement(17903..19027)
                     /locus_tag="OUD09_000808"
     CDS             complement(17903..19027)
                     /locus_tag="OUD09_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCT9692095.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
BASE COUNT         5941 a         3765 c         3190 g         6180 t
ORIGIN      
        1 tttttcataa tattcataaa aaattcgaat agctgctact aattttttgt ttttgttgct
       61 tgtcctgtct cataattgat cgtgtagcca tcttgaacat tgtagataaa gacattgaat
      121 tcaatttttt tgtcttcaat gctttctgct tccaattgca ctcccctggc taccagttca
      181 ttcccctcaa aatgaggagt gacacggtag cgtacatgat gatttgtttc tttgatatat
      241 gccgcaatat cattttcgtg agcaagcatg tatggagcat tcaacgaacg tgtccctgtc
      301 atcaagtttt taatgttgtt attttgacca gtcagctgga aaccaattaa atggctgcga
      361 ttatataacc attgcccgtc acttaatttc ttttgtttcc aacctgtcgg gtcaatatac
      421 aaaggctctc tcttttcagt agggaacaaa tcttttccaa gcatagcatt ggcttgtcct
      481 actctgttca atttatcaat atttgaatag ctttcccatg ttcctttgga aaggctaaga
      541 tcctcttttg taaattccgg ctgattccca ttgatttcta tttcttgatg ttttccatca
      601 tattctggca catccgctac ttgagaagct tgtggtgtgg aatcaaaaat agttgtaatc
      661 tgctgaaaga tatctgaatc tttttgtgtg gtggaaacac cgaatgcccc tgcaatcaca
      721 gcagcgacga tccccacgat cgccttggtt gtttttttgc tcatttcttt actcctttac
      781 taacagctcc ctaaatctta acatatgttc ccctataaat aaatactaga aagaaataga
      841 aacagcaaaa aataaaacct gttttaagat ggcttaaaaa tacaaaaaag agacggattc
      901 ctcgtctcta atcagtcacc actctatctg taaggtattt attattattc aaaaattttc
      961 ttcatactca agacaataca ctgatttcac aaccgcttgg taaacggtgc gcaaaagctt
     1021 gcatctgcgg taataagtcc atccaagtta aaaacataag aagttgtttt tgcttggctg
     1081 ttcttattac ttgttgcaga gcagcttttt cacaacctat tttaagcggg tgacgggaat
     1141 cgaacccgcg acatcagctt gggaagctgg gattttacca ctaaactaca cccgcaatac
     1201 aaaaacagta tacgcctctt ttattccttc ctcaagtttt tttattcttt acgcttctgg
     1261 tatgttttgt agtaaaataa aactaatcaa tcaattattg gagaagatgc tatgacggga
     1321 aaagaattag atctgttgct ggacacttgt ttactcgctg ggaaaatcat gatggaaagc
     1381 aatgcggaga tgtatcgggt agaagatacg atgagtcgaa ttgctttagc ttctggaaat
     1441 ttccgtttgg tcagctatgt cactcaaacc ggtctcttta ttggtcttga tcgtacctcg
     1501 accatccgaa tggaacaaat taccaaccgt tcgattaatt tagaaaaagt agtaaaagta
     1561 aatgatcttt cacgtaaata tgttgcttca gagctaacat tagaagaact ttactgcgaa
     1621 ctgcaagcaa tcgaccgaga ccgttctttc tttcctgtct ggttgcaaat cgttagcgcg
     1681 gcagtgatca gtggaacaat tatggtgtta tttggcggaa agatgaccga tttacctgtg
     1741 actttagtca ttggaggatt gggatacgca gcttatctat atagtttgaa attttttaga
     1801 atcaaatttt tatcggaatt tttatcttct ttattgattg gcatagccgc tatctacagc
     1861 gtacgttttg gcttaggaag caatcaggat ctaatcatta tcggatgtgt gatgccttta
     1921 gttcctggag ttcaaattac gaacgctatc cgtgatttgc tagctggaca ttatgtctca
     1981 ggagtttctc gcggaacaga agcgatgatg actgctacga tgatcggttt tgcgattgct
     2041 tttattttcc agctttttta ttaggaaagg aacacaagta tggggacgat atttattcag
     2101 ttttctttta gttttttagc gactgcggca tttgccgtta ttacaaatgt acctaggcgt
     2161 tcattgattt gttgtggtct ttctggtgct tttggctgga tgatctactg gatctgtgtt
     2221 caaataggcg gatctgctgc ttttggttca ttgcttggtt ctttaggagt agcattcatc
     2281 agcgatcttt tctctaaacg tttgaaaatg cccgtaacga ttttcaatat tcccgggatg
     2341 gttcctctag tccctggtgg tttagcttat caagcggttc gaaatctcgt tactgaatcc
     2401 tatcgagaag cggctcatta tgcggtccaa gcaattatga ttgctggagc aatcgcttta
     2461 ggtcttgtat tatcagaagt ctttaaccat aacatccgta atttcaaagt aaagcgtgac
     2521 gcaatcatga tgaaacgaaa aaaacggctt tttaaataaa cgaagtgtaa caaagaaaag
     2581 gttgtgaaag agacgttcag ccttgagcga tgacaaaata tctcacaaac attgcgacaa
     2641 gtaaccgcag gagcaataag aagtaattat gagtattaaa aaaagttttg taaaaatagc
     2701 ttctcacatt tttacataaa attgacttaa tactgagaaa ctgacttttg aacaccctct
     2761 atctgtaaaa aaggggctgc gacatgattt ttgtcacggc ccctcttctt tattcaaatt
     2821 ttttccaaaa cgttcgttga taataaacga tccatagagc ggaaagtatt agaactaaac
     2881 cgatcacagg aatattcact aaccaaaatg gataaaacaa agccaacatt ccgtataaga
     2941 cgataagaat aatactgatg atcaatgaac ccatcatagt tgcgactaaa cccaaacttc
     3001 ctgaacctct gttaaataat tggttaatat tggtccagtt cagcagcagc aaccgataat
     3061 ctctagcgaa ataacgtagt gacaataagt agcagccgaa taaagaacca aatagcaagc
     3121 tagggataaa aaacaaaggc agctgaaaag acagtccgct taataatgcg atcccaccag
     3181 ttaaaaaact ctgcaatatc catccgaatc tgaacttttc ttttaaatat tggttcatag
     3241 agattggcaa agaacggata aacagaaaat tttcttgatc caaagaaata agattgctga
     3301 taaaagaagt ctgattaact gttaagaaag ccaatgcgat ccctaccagg aaaacaacac
     3361 cgattaactt attatctaaa tcagttaatt ttatttcgcc tcctagggca aaagcaagaa
     3421 taaaaatgat tggagttaac aaggaagaag aaagaacttg cataatcaaa ttaggttctt
     3481 ttactaattg agcattgtaa ctaaataaaa gctgatgcac attttggttc tttttatggg
     3541 ttctttttat tttgaactca ctgtttgaat cagtcagttg ctctgtaagc tttggtacta
     3601 tcagctgttt tacagcaagg aaaaatccca gagccgtgat agctagtatt ccccaaccca
     3661 ataaaccttg ttgagaaagt ggctgactgg cagcatcata gaatggcaat aatatcgaaa
     3721 tcggatgccg atctgttaac tcattttcaa tagtccccgt ttgatgattc atccacatga
     3781 ttccagcaaa tgcgatcagc atcgagctgc caagcaagat actagaaact gtttttctat
     3841 gttttttaaa cactgctgtt cgagccaaac caaaaacaat caaactagcc agacaaaaaa
     3901 gaataatcaa aaatagagaa aacaaaagga ttgagataat gatagtaagc aaaatcccta
     3961 gatcagctcg ccatccggtt aaaataaaca tgactagaag cggaaaaaca aaaggaatga
     4021 tggtcaaagc aactaccaaa atctttgctg aaaaaacctg ttcttgtcca aaaggcagag
     4081 gcaaaaatac ttgcaggtca tttccttcaa aaaagatatt ataaatgact gatatacttt
     4141 gagttaaacc taacatacca aaaagtgcca cgtaataagt aaagaaacct ggcaatttaa
     4201 caaagttcat agcaaacata gcaaatccgt aaatcagtaa aaaaatcagc cctgaaaaaa
     4261 gattttgaga caaaagatag atagttaatc gctttccctt ttttcctttc tctcttgctt
     4321 tattcgtgag ctgtggatta acatataaaa gattaactcg aattaattct aataattgtc
     4381 gcttatacat acggtccatc tcctgtcgct tgccttccag ccattttcaa ataaatcttt
     4441 tctagcgttt catcaggtga gccattcaat aattcactaa ctgccccctg ataaatcaat
     4501 tcacctttct tcaaaatagc cagtttatcg cataactgtt gtgcggtatc caatacatgt
     4561 gtagaaaaaa tgactgtttt tccttttgcg gcatgagcct tcatcatttc tttcaaatca
     4621 aatgctgctt gaggatcaag tccttgcaaa ggttcatcca atacccaaat atctggatct
     4681 ggcagcaacg tgccgatcaa aatcgttttt tgacgcattc catgtgaaaa acttgcgatt
     4741 gtttcatttt gatgactata catatcaaaa agcgctgtca attctgctaa acgcttttct
     4801 tttttttgtg tgtctaattc ataggcagct gaaatcaagt cccaatattc tccagccgtc
     4861 aattgtaaaa aaatatctgg ggaatcaggc acataaccga ttttttgttt gatactcaat
     4921 cgattttccg tcaatttcat tccatctact aaaatcgttc ccgatgatgg ttcaataata
     4981 ctaactaaac ttttaatcgt agttgatttt ccggcaccat tatgtcctaa aaatccaaaa
     5041 atttcccctt gtcttatcga gagagacaag tcttttaatg cttgtttttt cccgtaactt
     5101 ttacttacat tgatgaattc aatcatttct gtactccttt tgtttgttat tactttttat
     5161 tgtacccttc gttattggtg gagtaaatgt caaaaagagt gcataacaaa aaaggataga
     5221 aacgactagg ctgctacttg ggttgttctc ttctacagga aaacactctt ctaacaggta
     5281 agatatgagt ctttgttttc tttaaaatga ttcattttct atcttttctt gcataaaatt
     5341 ctttagttta tcaatacttc tctttcccat ctctagttgt tctccatttg caaatgttac
     5401 tatttcgttt actctgctaa tagatgtaat gttttcagta ttaatgatat aagcttttaa
     5461 atctttacat aaatactcat attcgagtga ttttttatat tttttaattg tgccgtttac
     5521 gagaatctct tcatctttca atactaggcc tattttattt ttataacctg gcatgctcat
     5581 tagataaagg atttcatcgt aatggatata gtgatcgcta ttaccataac ggacagttgt
     5641 ccaccttttt ttcgtagcat gttgaacagg atagttttct ttttctttca ataagtaact
     5701 ttggatgctt tttttatttt tttcataagt gaattctttt atcaaataac cactaggcgt
     5761 aatctttcga ttaataacag ttattgcttt attcggatga gacgttagat aaacaatttt
     5821 acacttttca ttcttttctc taagcatttc gcctagttcg ataccgctat aataggcgtt
     5881 caaatcgatg tctaggaaaa aaatatctga ggaatagatt tcattatttt taatgaattc
     5941 aacaaattgt ataacgttca atccctgaac gatctcaaat ttccaaggac ttaataaaat
     6001 tctggattct tccaaaattt ctactagcat tttttcaaaa accaaactat catccaaaat
     6061 aaatatacgc gttaccataa aaagtacctc cactcgatat tttactatca aaatcatttc
     6121 atacaacttt tcaacataca tgttaggaat aaattgtaat tctcttcaca atcttttcca
     6181 acacctttga aaccgatatc aaatactttc aggatgctgc ttctaaaata aatatcaaac
     6241 aaatgaatac atcatgcaaa aaaagccaga caaccgacgt ccttgcgtgt cagctgtctg
     6301 agctatgttt atttattttg ttcttttaaa aagaacgata cacatactga tttttcggag
     6361 cagagacttt ctcagcgttt gtaacaacta cagatgggat ttcacgcttg aatggttgat
     6421 aatagtctga ttggatggtt ccttctattt tataccattc atcattttta accgttactc
     6481 cttccggcat atgcgtcaac aaaccaaaga ctcctgaatc tgccacacaa tggataatgc
     6541 cgaatcggaa caagaagata tctgtattgc tatcgttcgg tgtttggtaa acaaatcctt
     6601 catagctgat ttttttgcca atgaattcgc tagggtaatt ataaattagc tccataactt
     6661 ctaaatagtt ttcatctgtg atcttgatag tttgcttatc cccatacttt tctttcaact
     6721 cattcatttg gtctaagtag tctgatttgt tgaaataaat actggtatcc ggctttaagt
     6781 attgtgtctg catctccgga tcccctactg attctttact tactgggaaa ttaaatcctt
     6841 tggcttctac gatcgtagtg tcgagactaa ctgttggaaa caacgtcccc acgattactg
     6901 gcaaagctag cagtacataa gcgattccac gttgataagg ttttgacaac ccatggtcat
     6961 gatcgtgctc gtgtgccttt tcttctgctt ttccgttttt gacccacaaa tacagctgaa
     7021 ctaaagccaa aagagcagat aaaatcattg aaagaatagc taagtagcga taatggacat
     7081 tgatatattg atcgagtttg ccagaaactt gcaagtacat catcaattcg aaatacccag
     7141 ataaaatcaa aaaccgcagc atattttccc tcctaaataa ataatgtgaa tccaataacg
     7201 ctaagggcaa tcagtccgat cagcgataaa ataaagcgtc ctgtaaaata tcgtttcatc
     7261 atcagtagat ttttgatatc gaccattggg ccaaagacca aaaaggcagc tacaggtgct
     7321 gtgccgaaca gacttaacaa agaagatcct ataaacgcat ctgcttctga gcaaagagac
     7381 atcgtaaagg ccaataacag catgatgata atggctaaaa tctttgttga gccgagcgtt
     7441 agcataattc tagttggtaa ataagtctgc atcgcagctg cgattaatcc gccaatgatc
     7501 aaataacgac ctgtatcaaa aaattcgtcg attgaatgtg tcaacacatg cccagtttga
     7561 tggatcagct gattttttgg ttcattcttc tcactttgtt cttcttcatg gacatgatga
     7621 tggtgatttt ctgctacttt agacgtgatt ttttctttca atacagcgtc tttttgaaaa
     7681 taggcaagcc agacaccaat cacaagggca acaaaaaagc tgcctatcac ccgccagctt
     7741 gccatctgcc aagaattgcc aaatgcgata aaagtggaaa aaatcacgat tggattgatg
     7801 atcggagcag tcagcataaa agcaaacgct gtgtgaactg gtacgccttt tttaacaaat
     7861 tgatgaacga tcggtacgat gccgcattca catgaaggaa agaaaaatcc taagatcgaa
     7921 ccagtaaaaa tagagaagat acgattttca ggaagccatc tcttcacacg ttctggggtc
     7981 aaaaatactt gtaaggcgcc tgagataaga cagcctaata atacgaaagg taatgcttca
     8041 ataacgatcg ataagaaaat cgtactcatc tgcaaaaccg aatttggaat aaaagaaaac
     8101 atgagtttcc tcctttttat ttctagttca ttcgctttgc aaatatacgc ttttattcaa
     8161 aaaaaacaag ttgctctgta aatttttaca caactcttat tttttaccaa agcacttcaa
     8221 ttgacttttt ctgttattcc cattaaatta aggtctcata catatcactt ctatcataaa
     8281 agggagaata aaaatgaaac aaaccactaa cttgactgaa gttcaagaca tactgcaaca
     8341 gtcatccgtt gccgtcattt atctttcgat gcctaattgc agtgtttgtc acgctgttcg
     8401 tccacgacta gaggaacttc tcacacatta tgatattccg gcacttcatt tggatgcatt
     8461 tgaaacacca gaagtagcta gtaaatttga agtgctgact gcgccagttg tcctgatttt
     8521 tcatcaagga aaagaagtct ttcggcaagc acggttcatc gactttaaaa aaatccgtta
     8581 tctactagat gaactgacag cagaagatga ttccctttct tatgaagaaa tcttccgaac
     8641 aaaataatcg ttgtccaaac agaaaaaagc gattgaacaa gtgaaagttt tgccttcatt
     8701 tgttcaatcg ctttttattt ttattctgct gtattttcgg aatgcgcatt tgcgtcagaa
     8761 tgcaagaaat agatcaaggt ttgcaattca ttcgtcaaat caacattttg gatcaataca
     8821 tctgacggag cattcaatcg agcagcggta aagttcaaga ttcctttgat tcctgcttcc
     8881 gttaactgat tgactacttc ttgtgctctt tcagcgggta tcgttaaaat tgctacttcg
     8941 atttgttgga tcttgatttg ttctgacata tcttccattg gataaacggg gataccgtct
     9001 acgatgcgcc caacgatttc ctcattcaca tcaaaagcac aactgatacg tatgctatta
     9061 ctttgatgga atttgtattt tagtaaagca cttcccaagt tccctacacc gatcagcgca
     9121 acatttgtca attgatcgtc gtttaatgtc tttgcaaaaa agtgcataag atcttctaca
     9181 tcataaccat acccacgttt tcctaattcg ccaaaataag aaaaatctcg acggatcgtt
     9241 gcactatcta cttgaacggc ttcacttaat tcagttgaag acactttttt ctttccagca
     9301 ttatttaaaa tccttaaata gcgataatac aaaggcagtc ttttagctgt cgcctttgga
     9361 ataatatgat ctttcacgaa atgacctcca cgtattgtct aattacatct ttgatttctc
     9421 catcatagca gtatttatag gaaaaaatca attccattgc tcagatattg tgaaatttta
     9481 ttctttattc atttatttat catttctgca aatctcgcag tataatggaa tttatggtag
     9541 actactaatt gaaaaatagt tttttatttg aggagaattc acaaagtaga aaagaggatt
     9601 ccatgatatt attacaagcc aatcagattg ctcgccattt tggtgcagaa gtcttatttg
     9661 aaaacataca tttagaaatc agtacaggcg ctcgtgtcgc tttagttggc agaaatggcg
     9721 ctggaaaatc gactttatta aagatcattg ctggaattga agcacctgat gaaggaaaga
     9781 tcgctaaaaa taaaacagcg actctcggat acctcgctca ggacacagga cttgattcca
     9841 atgaaacagt ttggaacgag atgctgaaag cttttgaaca agttagacaa atggaagagc
     9901 ggatgcgcga gctagagatt ttcattagcg aaggtacgcc agacacacct gcttatagtt
     9961 cttatttaaa agaatacgac cggctgcaac acgatttttc agatcgaaac ggctatggtt
    10021 atgaaaatga aattcgttcc gtattgcatg ggtttaaatt cgattcttca ttttatgatc
    10081 agaaaatcga tactttatca ggcggacagc gcacacgttt agctttagct cgcatgcttt
    10141 tgcaacaccc tgacattttg attctagatg aaccaacgaa ccatttagat atcgagacac
    10201 tttcctggct tgagaattat ctgcaaagct atactggtgc attattgatt gtttcccatg
    10261 accgttattt tttagataaa gtagtcaatg aagtctatga attaagcaga agaaaaatgc
    10321 gccattacaa aggtaactat tcgaaatatc tagatttgaa agcagaacag ttagcaagtg
    10381 aatggaaggc ttatgaaaag cagcaaacag aaatccataa attagaagac ttcgttgctc
    10441 ggaaccttgt tcgcgcctct accacaaaac gagcgcaaag ccgcagaaag acattagaaa
    10501 aaatggaccg gctggatcgt cctcaagggg atgaaaaaca agcaaacttt ctattcagta
    10561 tagaaaagcc ttctggaaat gtagtcttgc aaatcgaaga tgccgctatc ggatatgata
    10621 atgtcacctt atctgaacca atcaatctag atatccgtcg tcaagaagcg attgctctag
    10681 taggtccgaa tggtatcggg aaatcgacgt tgctgaaatc aatcattcac cagatccctt
    10741 ttatccgtgg aaaagaaacg ctagggacaa atgtcaccat cggctattac gatcaaggtc
    10801 aagctgatct gcatacaaac aagacgatcc tagcagaatt gtgggatgag catcctacca
    10861 ctccagaaaa agagatccga agtgctttag gtgctttctt attttctggc gaagatgtag
    10921 aaaaaccaat ccctctgcta agtggcggtg aaaaagcacg ggttgcttta gccaagttag
    10981 cgatgaacaa agaaaatttt ttgattttgg atgaaccaac aaaccattta gatattgaca
    11041 acaaagaagt attagaaaat gcattgatcg attatgacgg cacactgctt tttgtttccc
    11101 atgaccgata tttcatcaat cggatcgcga caaaagtcgt cgaactctct gaaaatggca
    11161 gcaaagtcta tctaggagac tatgattatt atttagagaa aaagcaggaa gaagaagaaa
    11221 tcgccgaatt gttggcacaa gatgagccag aatctgctcc tgctcccacg aacaaatctg
    11281 attattacca aagcaaagaa cagcaaaagc ttatccgtag ccttcaacga aaaattactc
    11341 aggttgaaga agaaatggcg cggatcgatg aatggatcga tcagttggaa caagaaatgg
    11401 tcgcgccaga aaacctggaa gatcacgtaa aactcaatga gctgaaccaa gatttagaag
    11461 ctgctagaca agatcaagaa acaaaattga cggaatggga agaactaagc ttggaattag
    11521 aagaatatga aaattaacga aaaaaccaaa acagaaacca ttcatgctgt tttggttttt
    11581 ctttttttat tttatcaaac aaaaattcca tatttacctt tttcaccaat aagaagacca
    11641 attactagtc attctcacta aagcttctaa ataatagtaa tcaccccaaa tacaaggtgt
    11701 atttacccca ttatttttgg gcttattata aacagactgt gttaaaaagc catcttcttc
    11761 ttccgttgta gcatatcttt ctttcaattg tcgcataatt ttccaaacag cagactggta
    11821 taagttcttt tctgttttgt ctaatagacc tgattctatt aattctaata atccacaaac
    11881 catgatcacc atactagatg tatctttttc ttctctgcta gaatcatcaa agatcaaatc
    11941 ccaataacaa atggtatctt ctggcaaccg attcataaaa taattggcta atattttgct
    12001 taaatcaaaa tatttttgtt ctttcaaata acgataactt aatgcaaaac cgtaaatccc
    12061 ccatgcttgg cctctagccc aacaggaatt atcactataa ccttgatgcg tacttccata
    12121 tcttggtttt ccatctttga catccatata aaatgtatga taagttgaac catcttctct
    12181 tactaaataa tctgctgcat tttgaatatg agcctgagca gcttttttat atttttgatt
    12241 tccagtttct tcactcgccc aatacaataa tggaagattc atgcaacaat ccatgatcat
    12301 tctaccttgt tgtgaaggat cactaggatc tccccaagct tgtataactc ctgccttctc
    12361 ccaaaaacgc tgaagtaatg tttctgcagc atcaattgaa gcttccttca tttttcgatt
    12421 tcccgttatt ttataatctg ctacggttga taaagaatac aaaaaaccaa gatcgtgatg
    12481 attagtgata atttgacagt caatcctgtg gataaaatct atatctgctt tttcagctgt
    12541 ttgttgaaat aaaggatcac ccgtcttctc aaataacaac caattaattc ctgtccaaaa
    12601 tccttctgtc caaccaatat tgtctcttaa tggataacgt ccttttatag gacactcgct
    12661 tgggaattga tactgactgt tactgatacg tttgattaac ctctccgtgt gtctgcgaca
    12721 acgctcgatc tcattattcc attctttttc cattaattct ttataacatt catttatcca
    12781 tttttctgga tgtattatct cttcaaacat cactttatca agcatttccc caccagcctt
    12841 tagcataatt ttagtctttt tttattgttt ttattttgga tagttagccc ggttgtccca
    12901 tcaaaatcaa ttttgggacg attgttccat acttctaaac tataatcccg atcttgatga
    12961 gcaaccactc ctgttccgag ccaatgggtt ccttttccaa atgtaccctc taatccagga
    13021 ataaaagtgt tttcccatgt catcaagttt gtattgggca aacccataat agctgtggat
    13081 tttcgtttaa tagtatcgtc acttaaacag ataatacctg aaaagccagc ttcactcacc
    13141 ctatagtttt ccttgtcttc ttctatgatt attccttctt tctcaggaga acattcaata
    13201 gcaaaccctg tctcatatag aacatattct gtatctaatt ctactttgtg tacgcgaatc
    13261 tgccagcctt caaaaggaat aagccaagtt ctgatttttg cctgtaaagt aggttcccat
    13321 gtagaggcaa gtacctcttt cttacataca atgtctcgta cattatttcg aacgatgaac
    13381 tgtccatagg ttttgatgct ctcattctta aaactggtcg tgatatatgt gtccgctcgc
    13441 tgaatcccta gcattgaatc cattgccatt tctgctttca attgattcgc tcgcggtatc
    13501 gaaaaaccaa attgactaga ataagcaaat tttaaatatt tttcattggt caatgtatgg
    13561 aaatttggcc ctaattgtcc cgcattcaaa aaaaatgtat gtccattatc atgttgtatg
    13621 agcaaattgg cttctgaaag aagtttagtt ccttcacgaa taggcatagg catttcttta
    13681 gccgtccaat actcatggtc ttcaggtagc gccaacaata aaaaaatctt attcagccat
    13741 aatggagata acgtactatt atagggttca gtcaatgtca attgagggta ggtataacca
    13801 atattcagga tacctttttc atcaaatatt tctttttgca tccaccatct tatattccga
    13861 ttgactaacc ccttgatttc acctaattta aaaggcaaaa gatcggccca caacatagct
    13921 gaccaaaagc ttgaaactgc aaaacgataa atcatgcttc gaccaaaagg cacatttgca
    13981 ccatcttccg caaaaaaatg aatatagtct ctagaaaaat caatagctcg gttcttatat
    14041 atctggctaa tgggcttttc tggttctagt acgctataaa tcaaaccata ataatgaaat
    14101 gcaaaaggat tataataatc ttggcgacct cttgaactgt ctcgatacca gccattttga
    14161 agataacaat tttctatatt atcaaatgct tctttcagtg ctgattcttt aactggataa
    14221 ccaaattttt ggataattgt tccgacaatt atcttaaaaa attgccaatt accatctgca
    14281 cattcatatt caaaaatttg cgagaaccaa tgaataatct ggcttttttc tcctgtatct
    14341 aattttttcc ataataattg ttcatgatga tataacgcta ataacattgc gggcatttct
    14401 accatacgct ggtctctttc atgtatttct ccccaataat cgggatggga aggatctgtt
    14461 ccagctttta ttcctgatag aattaatttc aaccatcgtt catctaattg gtcaattgcg
    14521 ggccccgctc cccataaaac ccgagagaat ccttccattc ctgcaatttt atttccatat
    14581 cctacccctt gatgtatata atcaatttgt gcgccacttg ctcgataaaa cggctggagt
    14641 ggttgattta tctttttaaa taagtcaaag taatcctgtc tggtccttaa tagtggtact
    14701 ggatgtttca ttgtattttc tcctcttttt actaccaaat agctaattcc gactgttcta
    14761 atcgaagtaa gccttcaata aaaaaataat cagaatagat cagtggcaca ttataagtca
    14821 gttctctatg gtatgcttct gctccgcctc caatgatccc atcttcatcc atattccaat
    14881 ttgcatagtg cttttctgtc ttttggagta tattgatagc agcttcatga tacatttttg
    14941 atcgtacaga ggaaacatat cctgctaatt ccagaagacc gcatgcagcg catactccag
    15001 cactggtatc aatattcgaa tcttttacag caaaatcaat gagaggaata taaccagttt
    15061 tttcgatttt ctgaataaaa tttactgcta tcttttctgc tatagctaga tagtacggat
    15121 tttttgtgta acgataactt aacgcgaatc catataaagc ccatgcattt cctctgctcc
    15181 aacaagaatt ctcattgtat ccttgcccgc caagtaaccg aatatatttc cctgattctg
    15241 catcaaattc tgcgatatgc ccggttgatc catctttcct cactagatac ttagctgttg
    15301 tatctgcatg ctgccgagca attttagaga acctcggatc accagtttcc ctcgaagccc
    15361 aatacagtaa tggtaaattc atcatactat caataatcat ccaaccaggt ttatggtcat
    15421 tccatgctac taaatagttc cctgaaatat tatagcgtcc agcaagtacc gtagctgcat
    15481 gtaaccctag ttgtcgacta tgtttgtttt gagactgttg ataatgtgca atagccgatg
    15541 gtaagaataa aaaacctaca tcatgatgaa cgtctgaaaa atggtacagg gcagattcta
    15601 ataattgttc ttgttgtatt gctgcctctt tgaacttttc tcgttttgtc ttgtgaaaga
    15661 gctgccataa aataccactc caaaaaccat tggtccacca gtcgattcct ctgtccttta
    15721 ttacgtctgt atattttccg ttgtaagaaa tatatggaat tttattcccc attctgtcta
    15781 attccgcatc tatcttttca gaagctacat tcaaaatttt tcttgaccat tccttcgtct
    15841 gttcattcat ggatatcccg ccattctttt aatttttatg aatgtggagt tgctcctaga
    15901 acgaactaga tggttaacat tcgttatttt ttttacttat ttgcagctcc aatgtcattc
    15961 tccttaaatt tagactgcgc ccacacataa atcaagacca ttgggatcac tgaagttgct
    16021 aaaattagca taattgcgcc ataattaatt ggctcacctg tactagaaac cgtcatattt
    16081 ttgataatta agggaaccgt atatttatct ttatctgaaa gcatgactaa tggaaataga
    16141 tattgattcc aacttcccat aaagataagt aaaccactag taataatcgt aggtgataaa
    16201 atcggtacag cgattttcgt aaaaatcacc cactcattgg caccatcgat tctggcagcc
    16261 tccatcagtg ctgtaggaaa ttgactgcta tattgtcgca tcataaacac tgaagttgta
    16321 ctggcaatcg aagggatgat cacacctgca taagtattcg ataatcctaa acgactcata
    16381 atgtaaaata aaggaatcaa tgtggtaaaa aaaggcacca tcctagatag cataactgta
    16441 ttgaacaaca gtttattccc cataaaacga tactttgcta atgcataacc cgccattgta
    16501 gaactggctg tagctaaaac tgtactgatt acagcaataa caatcgaatt ccaaataatc
    16561 aagggataat tatatctcgc catgatggct tgccagtttt ttaatagcat atccccaaac
    16621 ttgaaattga ttccgccact taaaatttct tgattacttt gcgttgaagc tacaatcata
    16681 tataaatatg gcatcaaaaa tattaaggca ccaaacacaa taatgacgta cgtaaaaatt
    16741 ctgctttgta tcttttgttt agtcattttt atcacctccg aatttaaatt gaatagccga
    16801 taatataaca gatacaaaca ccatcgtcca ggcaatagca gaggcttttc caaaatcaac
    16861 catttcaaaa cttgttttat ataaattgac tcctacgacc aaagctgaat tttctggtcc
    16921 attggaagtc ccaaagaata tatttgggat attgaaatta cccaagccat tgatagtggc
    16981 taaaactgtg gccattaaaa tgattggctt tagcatgggg atcgttaata aaaagaattg
    17041 ccgaaatttc gatgctccat ctatttcagc actttcgtat atctctttag aaatcccttg
    17101 aagaccagct aaaaataaaa tagtgttata tcctacacta agccaaatca tcgtgatgat
    17161 cattgatatt cgtatccaaa aaggatttgt taaccactgt acaccgttaa ttcctagcgc
    17221 attcaaaaat ccattgaata acccagtcgg attgaaaaaa gtcatgaata cggaagctac
    17281 agccaccgtt gatgtaatgg ttggaatata atagacagtt ctaaaaaatc cttttccttt
    17341 taactgtgct gaattcaata aaacagcaaa gtaaatagct aatagcatcg ttattggaat
    17401 tgatcctgcc atgataatca aaacattcgt gtaagattta ataaacaatg gatctcggaa
    17461 gatagctaga taattgtcaa atccaacaaa ttcaaatgag tagccaattt tcataaaact
    17521 aaaagcaaac gagataatga tgggaatcaa aatcgtcaca ataaagaaaa taacaaaagg
    17581 tgtgaccaat ccataagcac tttttttatg agacgtatct gttgcttttt tcttgctgtt
    17641 ctttattgtc ttgcttggta acgaagattg catcatcgtt aagtcctcct aatatttttt
    17701 aatcaccagt taattttacg ccgtacttat gagcaaaatt ttctgcttct tttagatatg
    17761 aattttcaaa atcacctgtt tgccaatagt tatatgcagc ggtagtcaaa taagacgtag
    17821 catctgaata agtggctggt aaaacagtat ttgatgtatt ttcattgatt ttattcaata
    17881 gttcatagta tttttgtcct ccttagtagt caaacttttt ctcagctgct tctgtttgat
    17941 aggccaatga gttagatgta gccacgccca gctccagtgc gttggctaag cattcttcat
    18001 ctgtcagcat aaatgtaaga aattgttgtg ccacttgtgg atttttggca tctttaggga
    18061 cataaaaact tgatcctcct gatactgcac cttttcctgg attttctttt gaaaaaggaa
    18121 tcagtggagc catgcgccat ttgtcagatg catcaggaaa attcaaagac atattcgtag
    18181 cataccagcc gccagcaaca aacattgcag attcttgaaa tgctttctca gcatcttgtg
    18241 caccgtagaa atgtacaata tctgagtcga tcatttcttt gattaacttt gcagcatgtt
    18301 tcgcttcttt agtattcaga cggatatttc cttcttcatc aagcaaagat tgattctgct
    18361 gggctagcat gttgataaac aattcttggt ccccagattc tggcaaggca attccaaaac
    18421 taccagtttt ttcttttaat gcttttgcca cctggatata ttcttcccaa gaactgattt
    18481 cttcaaaatc tacacctatt tcagcaaatt tttttggttg ataataacaa agtgctgggg
    18541 taaaatcact agcccaagga ataatcgttt cattactcgt ttgttgacgc aaaatattca
    18601 atttttgatc ataaaactga tcgccatatt tgtcataaaa tccatattca tcagcactat
    18661 aaaaagcatc ttgaaatttt tccagcacac ctgctgctga agtgtctgcc atggagataa
    18721 tatccggcat atcttcgtgt gttaccaatt ttggggtgat catttgtact gttgctgttc
    18781 ccgaagctac attcacaatt tttaagtcaa tatttgctcc agttttgcgg ttatagattt
    18841 ttgcagcatt ttcaacatag tttgtcagtt tgccgtttgc gacatatact tctaatgtag
    18901 tggctttttt tatcattgga tctgcgatgt ttgctgttac ttcttcgtta gtttttccac
    18961 atgcacttaa gctgaataat agggtaatgt ttagcaaagc tatcaccaat ttgtatcttt
    19021 ttttcatgtc tatcccttct ttcttttcaa gttcttatat aaaacgcttt cactta
//
LOCUS       DAKBVG010000031        18475 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000031 DAKBVG010000000
VERSION     DAKBVG010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 18475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.031"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            299..1960
                     /locus_tag="OUD09_000809"
     CDS             299..1960
                     /locus_tag="OUD09_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HCT9692096.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(2030..2377)
                     /locus_tag="OUD09_000810"
     CDS             complement(2030..2377)
                     /locus_tag="OUD09_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HCT9692097.1"
                     /translation="MKKKSMPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2461..3402
                     /locus_tag="OUD09_000811"
     CDS             2461..3402
                     /locus_tag="OUD09_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT9692098.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3403..4446
                     /locus_tag="OUD09_000812"
     CDS             3403..4446
                     /locus_tag="OUD09_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT9692099.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4452..5522
                     /locus_tag="OUD09_000813"
     CDS             4452..5522
                     /locus_tag="OUD09_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT9692100.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5512..6465
                     /locus_tag="OUD09_000814"
     CDS             5512..6465
                     /locus_tag="OUD09_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCT9692101.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6477..7487
                     /locus_tag="OUD09_000815"
     CDS             6477..7487
                     /locus_tag="OUD09_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCT9692102.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7772..8593)
                     /locus_tag="OUD09_000816"
     CDS             complement(7772..8593)
                     /locus_tag="OUD09_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HCT9692103.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8690..8968)
                     /locus_tag="OUD09_000817"
     CDS             complement(8690..8968)
                     /locus_tag="OUD09_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9692104.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9236..11245
                     /gene="metG"
                     /locus_tag="OUD09_000818"
     CDS             9236..11245
                     /gene="metG"
                     /locus_tag="OUD09_000818"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCT9692105.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11724..12311)
                     /locus_tag="OUD09_000819"
     CDS             complement(11724..12311)
                     /locus_tag="OUD09_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9692106.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12486..13808
                     /locus_tag="OUD09_000820"
     CDS             12486..13808
                     /locus_tag="OUD09_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCT9692107.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13963..14739
                     /locus_tag="OUD09_000821"
     CDS             13963..14739
                     /locus_tag="OUD09_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HCT9692108.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14732..15301
                     /gene="rnmV"
                     /locus_tag="OUD09_000822"
     CDS             14732..15301
                     /gene="rnmV"
                     /locus_tag="OUD09_000822"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HCT9692109.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15294..16178
                     /gene="rsmA"
                     /locus_tag="OUD09_000823"
     CDS             15294..16178
                     /gene="rsmA"
                     /locus_tag="OUD09_000823"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCT9692110.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16629..17258
                     /locus_tag="OUD09_000824"
     CDS             16629..17258
                     /locus_tag="OUD09_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9692111.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17382..>18475)
                     /gene="ugpC"
                     /locus_tag="OUD09_000825"
     CDS             complement(17382..>18475)
                     /gene="ugpC"
                     /locus_tag="OUD09_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HCT9692112.1"
                     /translation="KNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGKSTTLRM
                     IAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGLKLRKYD
                     KADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMDEPLSNL
                     DAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIGTPKEVY
                     DTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGYEGKEVI
                     FGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKVDARDFH
                     KPGEIVDLAFNISKGHFFDKETEQVIKLP"
BASE COUNT         6040 a         3326 c         3768 g         5341 t
ORIGIN      
        1 tcagataagg gactgtgaca agacttttgt cacagtttct ttttgtttat tcttttttat
       61 ctttgtatgg caataataga caatataacg aaaattattt aatggctttt ggaagaccat
      121 ggaaaacctt gtacaaagct attttttctg aaaaattcct tgaaacctca cgaaaaaatc
      181 attattttat gaaaaagaca aaaaaaagtg ttgatttttt aataattatt cattaaactc
      241 ttaaacaact tcagaaaata ttaattattc tgaaaattcc aataaagggg tacgtaaaat
      301 gaaaaagaaa tttacatttg gtttggttgc ggtatgcggc cttgtacttg ctggatgtta
      361 tggcggcagt tcagccacaa gcagtagttc agcaaaaagc ggttcagcag atggaggagg
      421 cgtatttaac ttagtcgtgc ctcaagaaat gcctacagct gacttatcag ttgcaacaga
      481 tacaatcagt tttacagcat tgaataacgt ttatgaagga atttaccgtc ttgatgaaga
      541 cagcaaacca cagccggcag gagctagcga gttggcagaa gtcagtgaag acggattaac
      601 ttacaaaatc aaattacgag aagacgcaaa atggtcaaat ggtgatcctg ttacagcagc
      661 ggattacgtt tatggctggc aacgaacagt agacccagct acagcttctg aatatgctta
      721 cctgtatgcg ccagttgaaa atgcagaaga cattacagct ggaaagaaag ataaatcaga
      781 actagggatc aaagcagttg gtgactatga attagaaatc aaattgacga aacaaacacc
      841 atatttccaa tatctgcttg ctttcccatc atttttccca caaaaacaat ccgtagtgga
      901 agaaaatggg gatgcctatg catctgcaag tgataaagct gtttataatg gaccattcac
      961 attagaagat tttgatggac caggaacaga tacagaatgg acttataaga aaaatgatca
     1021 ttactgggac aaagatacag taaaactttc tgaaatcaaa gtcagtgtag taaaagaatc
     1081 ttctactgca ttgaacttat tcaaagatgg ccaagcagat gacgtgatct tgtctggtga
     1141 gttagcacag caaaacgcaa acgatccagc ttatacttct gttaaagaag cgcggacaag
     1201 ctatatcgaa ttcaatcaac gtgaaaagaa ttcaccattc aacaatgtga acctaagaaa
     1261 agctatttct tattctatcg atcgtgaagc tttagtaaaa caagtactag gtgacggatc
     1321 agttgtctct acaggattga tcccatcaga tatggtaaaa agtccaacaa cgaacgaaga
     1381 ctttgcaaaa gaagctggaa aattagtttc ttatgacaaa gataaagcaa aagaatattg
     1441 ggaaaaagcg aaaaaagaat tgggcgttga ttctttagaa tttgatttga tggcttctga
     1501 tgatgattca tcgaaaaaag taatcgaata tgttcaaaac tcaatccaag aaaacttgga
     1561 tggcgtaaca gtcaaaccaa cacctgttcc attctctgta cgtcttgacc gctcaacttc
     1621 gggtgatttt gacgcagtac taggcggatg ggctgctgac tatgcagacc caagcagctt
     1681 tactgatttg tttgtcacag gtaattcata caaccgtggt cgctggagca atgcagaata
     1741 tgacgaagca gttaaagcag cttcatcaac agacgctggc gatgaacaag cccgttggga
     1801 tgatttccaa aaagcaatca agatcatctc tgatgatatg ggtgtcgttc cggtgtacca
     1861 aaaagcagaa ggtcacctag tgaatgaaaa aatcaaaggt atcgttcacc atgcggcagg
     1921 cgcttcatgg gattacaaat ggacatacgt tgaagaataa tcgttaatac cgtgagcatg
     1981 agaaaaaacg aggctgcggc aaatgtcgca acctcgttct catattcact tatcctaaca
     2041 aagaaaaaag catgaaaata gcagaaagag caattagtat tccggcaatg atcagccaaa
     2101 agctgtacat accgtagctg gcagacgaga gcgaggaaaa ttctggtttt ttctttcggt
     2161 tccttgttcg tgcaagatgg accgatcgct gaaaatgatc gaagaaaccc gaagaaaaga
     2221 cccataaaaa accgccgata atcagaaacg gcaatgacca taagaataga tcattagata
     2281 attgaatgag tgtaaaggaa tggttgatga gattttttat taaaataacc agaatcagta
     2341 gaaaagctac tgcgtagggc atagattttt ttttcatttg attgctcctt tatttttggt
     2401 tcattatcta ataatgtagc gaaacatctg tcagaagtca aaagctaggg ggaaacatta
     2461 gtgaatagtt atatcaaata tgtattaaaa cgtgtctttt tcatgatcat cacgctttgg
     2521 ttaattgcaa caatcacctt tttcttgatg caattgctgc cgggcacgcc atatacgaat
     2581 caggaacgtt tgagtcctga aacaatcgag atgctaaata aacaagtcgg tttggataaa
     2641 ccagttatcg tacaatatgg gatttatctt tctaatttgc ttcaaggaga ttttggaatt
     2701 tccttccaat ttaaaaacca gccagtcgca catttgttag ctggaagaat cggaccatcc
     2761 ttgcagttag ggttacaggc aattatcttt ggaacagtcc tagggacgat tttaggaacg
     2821 atctctgcca tgaagcaaaa tacatgggca gatacatctt ctacactcgt agcgatatta
     2881 ggtcgttcca ttccaaactt tgtatttgct gttttattac aatatatctt tgcaatcaaa
     2941 ttacaagttt tgccaatcgc aaaatgggat ggctttgtgt acacaatctt accaacaatt
     3001 gcgttggcca tgtcgccgct tgctgactct gctcggttta tccgtacgga aatggtggaa
     3061 gtactgcata gtgattatgt ggaattagca aaagcaaaag gattgagccg ttgggaaatc
     3121 gcctttaaac atggtcttcg aaatagtttg attccattga tgactttatt aggaccgtta
     3181 gctgtagcat tgatgaccgg atcactggtg gtagaaaata tctttgctat tcctggtatc
     3241 ggggaacaat tcgttaagtc aatcacaaca aatgattatc ctacgatcat ggctgtaacg
     3301 atcctttact catttatgct gatttttgtt attttgatcg tggatctatt gtacggtcta
     3361 gttgatccaa gaattcgtgt ttcagaaggg agccgaggat aaatggaaat gataccaaag
     3421 cgttatgaaa cagttgcaga tatccctgcg gatgaatttc ttccattaca aaaaaatacc
     3481 gaagaagaac gtgaacagat tactgcacct tctttgaatt ttatccaaga ttcatggcgc
     3541 cgtttaaaga aaaacaaagc tgccgttatt tcaatggttt tactattggt catcattttg
     3601 atctctgtga tcacaatctt tgtttctcct catgatccaa ctgcccaaaa cgtggcttat
     3661 atcaatttgc ctcctcgtat tccgggaatc aatatcgatg gattgaatgg aaaagcaatg
     3721 gttggtggtg aacttgttga taaatatgct caagcaaacg tcccttcaaa cgtaaatttc
     3781 tttttaggaa cagacggttt gggacgtgat gtattaagcc gcttatttat gggaatgaga
     3841 atctcgttgc tgatcgcatt tattgctgca ttactagatg taacgattgg tgtagcttac
     3901 gggttggttt ctggtttgtt aggcggacgt gtcgataacg tgatgcaacg ttttttggaa
     3961 atcttatcag gaattccaaa cctcgttgtc atgattttga tgttggtcgt ttttgaacca
     4021 ggaatttttt caatcgtggc tgctatggcc atcacaaact ggataccaat ggcacgtatc
     4081 gttcgtgcac aaacaatgaa attaaaagat caagaatatg ttttagctgg attgacatta
     4141 ggtgaatcaa gaatgaaaat tgcattcaaa catattttgc cgaatatctc tagtgtcatt
     4201 attattcaaa tgatgtttag tattccatct gctattttct tcgaagcctt tttaagcttt
     4261 atcggtttag gtcttacgcc tccgtcagct tcattaggaa cgatgttgag cgaagggtac
     4321 aagacattcc tgtatctgcc ttatctatta tggattccgg cagtaacttt atcggttatc
     4381 atgattggct ttaacttact tgctgatgga ttgcgtgatg cctttgatcc taaaatgaaa
     4441 gagtgaagac gatgacgaaa attttagaag tgaaagactt agaaatctct tttgatacgt
     4501 atgctggaaa ggtacgagcg attcgcggtg tcaattttca tttgaataaa ggagaaacat
     4561 tggcgatcgt tggggaatct ggaagtggaa aatcagtgac gaccagaagc atcatgcgct
     4621 tgctgtcaag caacgccaat atagatgctg gtcaaatcct ctttaaaggt aacgatatcg
     4681 ttggaaaatc agaaagagag atgcaaaaaa tccgtggtaa agaaatagcg atgattttcc
     4741 aagatccaat gacctcttta gatccgacga tgcccattgg gaagcaagtc gctgagtccc
     4801 ttagaaagca taataaagtt tcaaaaaaag agggacaaaa agctgcatta gaattgctta
     4861 aattagttgg aatacccgaa gctgagaaac gtatcaacag ttatccgcat cagttctcag
     4921 gaggtcagcg tcaacggatc gtgatcgcga ttgcactggt atgttatccg gaaatcctga
     4981 ttgctgatga acctacaaca gcgcttgatg tgactattca agcacagatt ttggagctgc
     5041 tgaaagaaat tcagacaaaa atcgatacat caattatctt tatcactcat gaccttggtg
     5101 tagtagcgaa tgtcgctgat cgtgtcgctg tcatgtatgg aggaagaatc gttgaagttg
     5161 gaacatcgga agaaattttc tacaacccgc aacatcccta tacttgggga ctgcttggtt
     5221 caatgccgac attagatagt gcgaacgatc gattgtatgc tattcctggt tctccaccgg
     5281 atttattgaa cccgccaaaa ggagacgcat tctatccaag aaatgaattc gcgatgaaaa
     5341 tcgatgcaga acaagaacca ccattttttg aactatcaaa aacacacaag gcagcaactt
     5401 ggctacttgc tccgcaggca cccaaagtga caccgccaga agaaatccaa cgtcgctggg
     5461 ctatctttaa agagaaacaa aaacatactt acggtcaagg aggaattgct gatgccaaat
     5521 aaactacttg aagttaaagg attgaaacag tatttcaatg taggaaaaaa gaatgaagta
     5581 catgctgtaa atgacatcag ctttcacatt tatgaaggag aaacattcgg tttagttgga
     5641 gaatctggaa gcggaaaatc cactactggc cgtacgatca ttcgcttgaa tgaacctact
     5701 gatggtgaga tattattcga cggtcaagat ataacgaaga tcaaagataa aaaaggtttg
     5761 acgaaattcc ggcatgatgt tcagatgatc tttcaagatc catacgcttc gttgaatcct
     5821 cgtatgaaag tcagagatat cattgccgaa ggaattgatg ttaatggact agctaaatca
     5881 tctgaagagc gtgcaaaaaa agtcgatgat ctattgagaa ctgttggatt gaatccaagc
     5941 cacggtactc gttatcctca tgaattttca ggtgggcaac gccagcgtat tggtattgca
     6001 cgagcattgg cagtaaaacc gcgttttatc atttgtgatg aaccaatttc tgcactagat
     6061 gtgtcgatcc aagcccaagt ggtcaatttg ttgcaagatc tccaaaaaga acatcaattg
     6121 acctatctat ttattgcaca tgatttgtcg atggttaaac atattagtga ccgaatcggt
     6181 gtaatgcata acggcttgtt attagagatg ggaacaagtg atgaaatcta taatcacggt
     6241 gttcatcctt atacagaaag tttgttatct gctattcctc taccagatcc agaccatgag
     6301 agaaaacgca gaagaatcaa atatcaacct gaaccagatg acggacaagt acgtcaactt
     6361 cgtgaaatcg caccagaaca ttttgtctat gcaactgagc aggaagtccc atattacgca
     6421 aaaaaactaa aacgacaaaa ggaatctctt ctggttgcta actaggaggg acatagatgc
     6481 taaaagcttt taaattccgt atgtatccga cagaagaaca aaaacagcaa ttgattcgta
     6541 cttttggatg tgctcgattt acctataacc atttgctgaa aaagcggcag aaaagttggc
     6601 aacaaacagg agtagccaat ttttcgctca cacccgctac attaaaaaaa gaatacccat
     6661 tcttaaaaga agtagacagt cttgctttag caaatgcaca gcttaatttg gatcgggctt
     6721 ttcgcaatta tttcaaggga cgtgccagct tcccaaagct taaaacgaaa aaaagtatgt
     6781 ggcaatctta tacgaccaac aaccaaaccc gtacgatcta tctggaaaat ggctacttaa
     6841 agttaccaaa acaaaaagaa ctaatcaaga taaatcagca tcggccagtt gaaggatcga
     6901 ttcgctccgc tacgatttct gctcgatata atgaagaatt ttatgtcgca ctactttgtg
     6961 atgtttcgcc agtaaaaaaa gaatctttag ctaaatggat cgggatcgcc tatcatccga
     7021 agacattgat ccaaacatct cgacctctag aagtaacttt accaaaattt caccagacag
     7081 aagaaaagct gcaacatgca cagcggaaat tgaatgtgaa agtccgctcc gctcaccata
     7141 gaaagatcag gttagatcag gcaagcaatt atcagaaaca aaagagaaaa gtaatggatc
     7201 tttatctgaa acaaaagaac cagcgagaag attatcttga acaattatct gggaaattgg
     7261 tcaaacaata tgactactta tttgtagaat cttttccaaa agaagaggca catgctgatt
     7321 tttctattca cgactggcat aaattaataa caaaattgcg ttataagtcc caatggtata
     7381 ataaaaaatt tcttttgatc aataccgacg gagcagaaga aagcaattcc gtaagaaaga
     7441 gccaagtagt agaaaaaatg ggcagacatt cggttatcaa gggataaaaa atgaatgaaa
     7501 ctgataggag cgttagggtt agccttgtta ataaaaagtc ttaatgactt ttgtttctag
     7561 gaatttttct tttgaagaaa agctcactaa ttatgacaaa ccaataaaac aatagcagaa
     7621 aaagttcgta cgagcttttt ctgctattgt taatttttta ttcagaaagt ttacgaatac
     7681 atcaaaattc tgaaacaaaa aacgtaactc acaaagagtt gtttttgcgt tttttcttga
     7741 atgattaggt ctgaatcttt tgctcacaac cttattcatg gtgccccggc ttgagctgat
     7801 agatttttga aggacggccg attcctacag gacgttcacc gatctcatcg aaatattgga
     7861 gcatagcttt cttaaaattg gaatgatcga tcgttttata atcgatccct aagaatttag
     7921 caaatacttt tcgagcttcc gtaatcgtga aatctttacc taaaacttgt agaacttgcg
     7981 gctcgtgttc cattttattg actacacggt tgaatgcttt caagatgatt tgactatggt
     8041 caaaagccaa agtatctttg ccagaagact ctccagtgac taaatcaagc gagatagcca
     8101 cttctccgct agataaatgg atcttgttac tatgacgttc tagtgtgaac cagcgtactt
     8161 ccttggcatc atctccggcg attagcggtt cttctccaat aaaggctaag tagctgactg
     8221 tgacgaccca gcctcgcgga tctcgattag gcgtactgaa tgtatgcagt tgctcaatat
     8281 tttcttttga aataaccaca ccagtttctt cctttgtttc tcttaagacg ctttctccag
     8341 ttgactcatc cttttggaca aagccaccag gcaatgccca tgaatttcga taaggatgcc
     8401 cttttctttg gatcaacaaa actttcagct gatcttcttc tttgttatag cacatcagta
     8461 cgatatcgac agtcaaagag ggcttgtcat attctggacg ttcctgtttt tgataccagt
     8521 taagaaattc ttcttcactt gctttatgtt cataatataa tttttcttct tctttagaag
     8581 caaaacggtt catctgacca cctccattga cagtaaaagt actttttacc ttattgtagc
     8641 agactctgaa aaaaaaccaa ggatttactt gaaaaatcta ggttatcatt taaatatgaa
     8701 aaataaatgg aagtatgaga ttgaataaaa agaaaagcag ataaaacggc aatactttat
     8761 accgctcttc acgaatggca aagaagccac aaataacgat atatacgtca atgataaaac
     8821 gcaaaatcca agctgctgtc ataaaactgt tcaaatgccc atggaggtta aaataaccat
     8881 acatacgaat ataaaaatag ctgaatatta aatgtgctaa tgaaagaaaa gcaatactaa
     8941 gtgtaaaaaa aagatgtttc ttctgcatga ttgttccagt cccttactaa gatagatagt
     9001 ctttattata ccacgtgtga ggggagaaat aaaaagccgt tttttagtag atatgaaaca
     9061 aaacaaaaac ccgcatttat tcaacctatt catcctttta aaggatagat tgaacgtaga
     9121 tagaagtcgt ttatccagtc tttttcattt tgaaaaaaag ctaacagcat gcttttttta
     9181 tggtatacta gcaaggaaac gaattcgcat catgcgataa aggaggaaga gccccgtggc
     9241 agaaaaggaa actttttata tcactacgcc aatctattat ccaagcggca aacttcatat
     9301 cggcaactcg tatactacga ttgcatgtga cgcaattgct cgttataaac ggttgatggg
     9361 ctttgatgtg ttttatctca ctggagtgga tgaacatggt caaaaaatcg aaacaaaagc
     9421 agaagaacta ggtgtaaaac cgcaagaata tgtagataaa atggcggctg atgtcaagaa
     9481 attatggaaa acgcttgata tcagttatga taaattcatt cgcacgacag atgattacca
     9541 taaagcagct gttcaaaaaa tatttgatcg tttattagaa cagggagata tttacttagg
     9601 tgaatatgaa ggctggtact ctgtttctga cgaagaattc tttacagaaa cacaacttgc
     9661 agaagtgtac aaagatgacg aaggtaacgt gatcggtggt aaagcgccaa gtggacacga
     9721 agtagaattg gtaaaagaag aatcatactt cttccgcatg agcaaatacg cagaccgtct
     9781 attggaatac tatgaagaac atcccgaatt catccagcca gaatctcgta aaaacgaaat
     9841 gatcaacaac tttatcaaac caggattaga ggatcttgca gtttcacgaa caacttttac
     9901 ttggggagta ccagtcaaga gtgatccgaa acatgtggtg tatgtatgga tcgatgcatt
     9961 atccaattat attactgcac taggatatgg ctccgaagat gaaagtctat tcgaaaaata
    10021 ctggcctgca gatgttcaga tggtcggtaa agaaatcgtt cgctttcata cgatctattg
    10081 gccaatcatg ctgatggccc ttgatttgcc tctacctaaa aaagtatttg ggcatggctg
    10141 gctattgatg aaagacggaa aaatgtctaa atcaaaagga aatgtcgttt atcctgaaat
    10201 gcttgtggaa cgttatggtt tagatgcact ccgttattat ttgcttcgtg ctgttccgtt
    10261 tgggtcagat ggtgtcttta cgccagaaga ttttgtttcc cgtgtcaatt atgatctggc
    10321 aaatgatcta gggaacttat tgaatcgaac gattgctatg atcaacaaat attgtgatgg
    10381 actcgttcct gactatgcct cacttgtcac tccatttgat agtgaactgt ctacgacggc
    10441 tgctaacgtt gtcggacgtt accatgatgc aatggaaaaa atggaattca atacagcatt
    10501 ggctgaaatc tgggtgctga tttctcgtgc aaacaaatac attgatgaaa cagaaccatg
    10561 ggtattagca aaagacgaag aaaagaaagc tgaattgaat agtgtcatga tccatttagc
    10621 tgaatctttg cgtatcgcag cgatattact tcagccaatc atgacagaaa cgccagagaa
    10681 gatcttcaat caactaggat tggactctga aacaatgaat cttgaaggat tacactttgg
    10741 cgaatttcct tcaggaacaa aagtagttgc aaaaggtaca ccaatcttcc cacgtttaga
    10801 tatggaagaa gaagttgctt ttattcaaga aaaaatgtct gaaggtactc aaacaaacga
    10861 agatacagtc aaatgggatc cagaagaaac agaacttgtt tcaacgaaag aaaaacaaat
    10921 caaatttgat gtatttgaaa aagtagaact aaaagtagca gaagtcatca actgtcaaaa
    10981 agtagaaggg gcagataagt tgctgcaatt ccgactagat gctggagaca gccaagaccg
    11041 tcaaatcttg tcaggaatcg ctgaatttta tcctgatcca agcgaattga tcgggaaaaa
    11101 agtcgtcatt gtagcaaatc tgaaaccacg aaagatgcgt ggacaaatca gtcaaggaat
    11161 gatcctttct gcagaagcac cagatggctc tttacaagta atcgaagcac caaaatccat
    11221 gccaaatggc tcagaaatcg catagaaatt caaagggggt atgacactag ttgtgtcata
    11281 cccccttttt ccgaaatttg agaagcaatt ttcgtaaatt ccttcttatt acttgaagcc
    11341 aatcatataa tacagaaaca gagaactatc caaaataaat cctagaaaaa gaagtacgag
    11401 cttcgctgat ttttgttaat ttagaataat actaatagga atcgtaaaaa cagaaaaaaa
    11461 gcataatgat aaagggtcgg gcagatttta attgattcat ctggtactct tttttattgt
    11521 ccttacattc ataaagtttg aatgcaatga ctttattaca tctatattta tatctaacag
    11581 tagcgttgat gtgttaatta taagttctat cactttatgt tcaattagtt tgtgctctta
    11641 tatgtgtgat tgaaaatata gataaaaaaa caatcatcgg ctttgacttc gatgattgtt
    11701 ttctttattt tgtaaaaatc ctatcacttg attttatggt aaccttttat ttttgtcttc
    11761 aaacggcttg gtaagagagc agtaagcagt gcaaatggga tcgaaaagac ataccagaaa
    11821 atcgtacgtc cgataccata atgttgatag agctttgtca acatcacgtg taacaagatc
    11881 aaccctaaaa gcaaggagcc agctataaac caatgattat cccaatgcat gacttcacta
    11941 agtgccatca ctccaaaagg atagaaataa aaatagatcc cgactaaggt catccgccag
    12001 ttctttgtaa atgttccgat attcaataaa aagaaaatcg ctgaaagcaa aataccgata
    12061 ggcggaaaaa gcccaaccaa aaaggaatac aacatccccc acaagatcat cacaggacct
    12121 ttgatcaatg caacgattgc gatgaacaaa gtcagcaata ttagtttttg tgataacgtt
    12181 gcaacagtca ttaatccaaa taaaattaat gtgaaccaat gccaatggga ctgctgttgt
    12241 tctttacggg aaaaatcgac ccggttggat aaataatctg ctaatttcgg ataagaacgc
    12301 ttaaaatcca taaactgttc ctccttcgtg gaaaaggtag gtttagtata cgaaaaataa
    12361 aaaagaaata catccgtctt aagagggagt ttttcactga aagtggagaa aaaagaatgg
    12421 taaattaaaa tattctcagt tatactgaaa attataccaa atttcaaaga agaaagagga
    12481 tgggaatgaa aggcaaacta aaacgagaaa tcaatctgtt tggcgcatta gctaccgtta
    12541 tgggaaccgt gatcggtgca ggcgtcttct tcaaaactgc tgctgttact gctagtactc
    12601 agtctacaag tttgacattg ctagcttggc ttttgggtgg tttcttaacg atctgtgctg
    12661 gattgacagt ggctgaatta gctacagcaa tcccggaaac tggtggagct gtaaaatata
    12721 ttgaagctgc gtatggaaaa ttgcccagct ttttattagg atgggcgcaa agcttgattt
    12781 actttcctgc taatattgct gcattatcga ttatctttgc gacacaattg acgaacttat
    12841 tacaattatc taccgattat cttttgttga ttgcagtgat caccgctgtt tctgtgacag
    12901 ggttgaattt gttagggaca aaagtcggtg cttctgtaca atcagtgacg ttgattgtta
    12961 aattaatacc aattgctgtg attgtcatat ggggattatt gaccccagga caaggaactg
    13021 tccagttatt tccaatcgaa gctggaaaag atgttacttt tgtcgaagga ttgagcagtg
    13081 cgttgttagc aacgttgttt gcttatgacg gctggttagg tgtcggtgca atggcagggg
    13141 aaatgaaacg gccggaaaaa gatttgccta aagcaattat tcttggtttg agttttgtaa
    13201 cagtcgttta tctattgatc aattttgttt ttttgaaaac tttgccgatt gatcatcttg
    13261 ccggtaattt gaatgcagct tctgaagcat cagacgtcat ttttggagga atcggtggaa
    13321 agctggtcac tattgggatc ttgatctcgg tatacggtgc attgaacggt tatacactaa
    13381 ctgggatccg cgtgccttat gcgatggctt tagaagacga gctacctttc agtaaacagc
    13441 tgacaaagct ttcaaagaag tttacggttc cttatgttcc tgcggttttc cagttagcag
    13501 tagcttgtat tatgatgagc cttggttctt tcgacttttt aacagacatg ttgatttttg
    13561 tgatgtggct attcagttta ctgattttta tcggtgtctt tattttaaga aaaaaagcgc
    13621 cggaattgcc acgtccatat aaagtaccac tttatccaat cgttccgatt attgcgattc
    13681 tcggtgctat tttcatttta ggtatgacga tgatgacgca aacgaaatta gcattgatcg
    13741 gtataggtgt taccttgata ggtatcccag tctattatca aaaaaagaaa aaacgctcag
    13801 aagagtaaaa tatttcaaca acagacagaa aaagtacgaa ctaggtcctc tagatcttcg
    13861 tgctttttct atttcaataa aacaagttct tctattttga tttttgctac ttctgcaagt
    13921 agtgcttatg tgctaaagta agaatgaaaa ggagagataa acatgatatt tgattctcat
    13981 acccatttaa atgctgaaca gttcaatgaa gatattccag aaacgattca gcgagcacaa
    14041 gaaatggggg taacgaaaat ggcggtcgtg ggcttcgata cgccaacgat tgaaaagtct
    14101 ttgcaattaa gccatgacta tccaaatata tacagtatta tcggctggca tccgacagag
    14161 gctggcagtt atacgaaaga aatcgaaaat aaattacaag aacaattgac tttaccaaag
    14221 gttgtagcat taggggaaat tggcttggat tatcattgga tggaagaccc gaaagaagtc
    14281 caagataaag tatttcgcag acagattgct atcgcaaaag aaatgaacct accaatcagt
    14341 attcatacta gagaagcact tgaagacacg tatcaaatcc taaaagaaga agatgttcga
    14401 gatatcggtg gaatcatgca tagtttcagt ggtgattatg aatgggcaaa acgatttctt
    14461 gacttaggga tgcatatttc atttagcggt gtcgtcactt tcaaaaaagc attagacgtc
    14521 caagaggctg cagcaaatgt accaatcgaa cgattacttg tggaaacaga tgccccttat
    14581 ctggcaccag ttccttttag aggaaagcga aatgaaccag gctatactcg ttatacagta
    14641 gaaaagattg ctgaacttcg gaatcttccg acagaagaag tggctgccca aacatggaca
    14701 aatgcgcacc gattatttgg tctaagcgaa catggataag atgaagatcc aagagattat
    14761 agtcgttgaa ggaaaagacg atacaagaag gctgcgggaa gtagttgatg cagatacgat
    14821 agaaacaata gggtctgcaa tcaatgaaga gatcctgatg caaattgaac atgcccaaga
    14881 gactcgtgga gtgatcgttt ttaccgatcc tgatttttca ggagaaaaaa taagaaaaat
    14941 cattatggaa gctgttcctc aggcaaaaca tgcttttttg ccgcgcacac aagctaaacc
    15001 aaagaaaaag ggaagccttg gtgtcgagca tgcaagtgat gctgctattt tagaagcatt
    15061 gaaaaaggtc gtgacgccag acatttccca aacggctgtt tctgaaatca ctagacaaga
    15121 ccttttagac tatggtttaa tagctggggc acaagcaaaa gaaaaacgag aaaaactagg
    15181 tgacgagttg cgcatcggtt acacgaacgg caaacaattg gaaaaacgtt tgaagatgtt
    15241 tcgtattacg ccgaaagaac tcgcccaagc aatgaaaata gtggaggaac aacatgagtg
    15301 aatatcgtga aattgcgaca ccctctagaa caaaagagat tttattgaaa catggttttt
    15361 catttaaaaa aagtttagga caaaattttc tgactgaacc aaatatttta aggaaaatcg
    15421 tagaaactgc gcaaatagat gaacatacga acgtgatcga agttggacca ggaatcggtg
    15481 cattgacgga acaattagcc aagcatgcaa aacaagtcgt agcatttgag atcgatgatc
    15541 gtttgatccc tgtattggct gatacgatgc aaccctatga caatgtgact attgtacatc
    15601 aagatatttt aaagaccgat ttgtcaacag ctgttagaga aacatttcat gaggagcttc
    15661 cgttaaaagt cgtagcgaat ttaccttact atattacgac accgatcatg atgcattttc
    15721 ttgaatcaga tttggttgtg gatgaactag tcgtcatgat gcaaaaagaa gtagctgatc
    15781 gtatctctgc tgaaccagga acgaaagctt atggttcatt atcgattgct gttcaatatt
    15841 atatggaagc cagtttagca ttcatcgtac caaaaacagt atttgtccct cagccaaatg
    15901 tagattcagc gattttgaaa ctgacaagaa gagatatacc ggcagtcgaa gtgacagacg
    15961 aaaaagcatt tttccgattg acaaaagcgg catttcagca aagaagaaaa acgctttgga
    16021 ataacttgca gcattcgtat ggcaaagatg accagacaaa agcatggtta gcaaaaagct
    16081 tggaaacagc tggaatcgat ccaaaacgtc gtggggagac tttgtcatta caagaattcg
    16141 ccgcattaag caatgcgatg tcagaaaata tgcagtaaaa aaagaggttg tgacagaagt
    16201 tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca aacaaaaata gttcctcatg
    16261 tttttcgttt gtttgggctt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    16321 aggttgtggg cctgccgttt aactctaagt cacaaggaac aatttcaaaa aacagcttct
    16381 catgtttttg agttagttgg tcttattact tgatgcaagc ttttgaccac tattatgccg
    16441 aacaaggaga tgtgacacaa attttgtcac atctcctatt tttgtttctt gatcaaaaaa
    16501 ttcttatact tgatactcac actcattttg tatggattta gaaaagaata tgtgtcttga
    16561 cagttattag taagtgagtt atactaagtc gacatttatg tgtcaagaca tattaggaga
    16621 gagaaaaaat gagaaaaaat tcagttcgaa aactaacgat ttctgcttta ttgattggga
    16681 tgggggtcat tatcccaatg gtcatgccta aaatcatgat tccaccagct tcctttactt
    16741 tggcgtctca tgtgccatta tttattgcga tgttttttac accaggagtt gcagtggctg
    16801 ttgcgctagg tacgacattt ggtttctttt taacaacgcc ggttatcatc gcattgcgag
    16861 cattgtcaca tcttgtgttt gcgttgattg gggcttggta cttgcaaaag catccgagta
    16921 tcgttttaaa aaatggacag tttacttttg ctaattggcg tttccaaggg ttcaattttg
    16981 tgatcggtgt gattcattct ttagcagaaa tgctagtagt cagcatcttt tactttattg
    17041 gtaatatgcc tgaaacatat tattctcaag gatattttta tacagtattc atcttgatgg
    17101 ggattggtgg gcttatccac agtttagttg attataatat tgcttatttt atagcaggaa
    17161 cactcagcaa atattttgat atccccgtat ttacggcagc aaaaaaagaa aatcggaaag
    17221 agtcgattca agggtacgcg aagaaaacga catattagac atgatctctc aaaaaaaaaa
    17281 ctctcatcta gtaatggata ataaatcctc taagatgaga gttttttgtc ctaaaaagag
    17341 ctgcaccaac atcttctggc acagctctaa cagggggcaa tttatggaag tttgatgact
    17401 tgttctgttt ctttatcaaa gaagtggcct ttgctgatgt tgaaggcaag gtcgactatt
    17461 tcgcctggtt tatggaaatc tcttgcatct acttttgaga tgaactcagt agaaccagtt
    17521 tttgtgtaaa gcatcgtttc agctccaagt aattcggaaa cgacaacttc tgatttcact
    17581 acggcttcag gtgaagcttc aagggctact tgttcactgt ggatgtcttc tggacgaata
    17641 ccgaagataa cttcttttcc ttcataacct ttttcaacta atactttgtt ttttccttca
    17701 ggaatacgaa gcttaagccc atgtccatct gtgataacac catttttcaa tgtcacatta
    17761 aagaagttca tggcaggaga tccaataaag ccggcaacga acatattgac gggtgtatca
    17821 taaacttctt tcggtgtacc gatttgttgg ataaatccgt ctttcatgat aacgattcgg
    17881 tcagccatag tcattgcttc tgtctgatcg tgagtaacat agatggtcgt tgtttcaaga
    17941 cgctggtgaa gtttggcaat ctcagcacgc atggctacac gtaatttagc atctaaattt
    18001 gataatggct catccattaa gaagactttt gcatcccgaa cgattgcccg tcccaatgcc
    18061 acacgttggc gttgtccacc agataaagca gctggttttc gtttcaagta ttctgtcaga
    18121 cctaagatct ctgctgcatt gtccacacgt tttttgatgt ctgctttatc atatttacgt
    18181 aatttcaagc caaaagccat attatcaaaa acagtcatat gaggatacaa tgcgtagttt
    18241 tggaaaacca tggcaatatc acggtcttta ggtgccacgt cattcatgac ttttccgccg
    18301 atatttagtt ctccttcaga gatatcttcc agtcccgcga tcattcgcaa agtagttgac
    18361 tttccacaac cggaaggacc aacaaaaacg ataaattcac ggtctttgat tgatagatta
    18421 aaatcagtta cagaataatt ttctgcattg tcatattttt tatatacgtt tttca
//
LOCUS       DAKBVG010000032        18139 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000032 DAKBVG010000000
VERSION     DAKBVG010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 18139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18139
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.032"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(46..1026)
                     /locus_tag="OUD09_000826"
     CDS             complement(46..1026)
                     /locus_tag="OUD09_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCT9692113.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(1050..2534)
                     /gene="galT"
                     /locus_tag="OUD09_000827"
     CDS             complement(1050..2534)
                     /gene="galT"
                     /locus_tag="OUD09_000827"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HCT9692114.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(2546..3535)
                     /gene="galE"
                     /locus_tag="OUD09_000828"
     CDS             complement(2546..3535)
                     /gene="galE"
                     /locus_tag="OUD09_000828"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCT9692115.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            3783..3950
                     /locus_tag="OUD09_000829"
     CDS             3783..3950
                     /locus_tag="OUD09_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HCT9692116.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(4012..5823)
                     /locus_tag="OUD09_000830"
     CDS             complement(4012..5823)
                     /locus_tag="OUD09_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HCT9692117.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(5820..6185)
                     /locus_tag="OUD09_000831"
     CDS             complement(5820..6185)
                     /locus_tag="OUD09_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HCT9692118.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(6348..6743)
                     /locus_tag="OUD09_000832"
     CDS             complement(6348..6743)
                     /locus_tag="OUD09_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCT9692119.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(6761..7723)
                     /locus_tag="OUD09_000833"
     CDS             complement(6761..7723)
                     /locus_tag="OUD09_000833"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HCT9692120.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(7723..7935)
                     /locus_tag="OUD09_000834"
     CDS             complement(7723..7935)
                     /locus_tag="OUD09_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HCT9692121.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(7956..8654)
                     /locus_tag="OUD09_000835"
     CDS             complement(7956..8654)
                     /locus_tag="OUD09_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HCT9692122.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(8674..9216)
                     /locus_tag="OUD09_000836"
     CDS             complement(8674..9216)
                     /locus_tag="OUD09_000836"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCT9692123.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(9348..10352)
                     /locus_tag="OUD09_000837"
     CDS             complement(9348..10352)
                     /locus_tag="OUD09_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCT9692124.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(10349..11338)
                     /locus_tag="OUD09_000838"
     CDS             complement(10349..11338)
                     /locus_tag="OUD09_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCT9692125.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(11335..12141)
                     /locus_tag="OUD09_000839"
     CDS             complement(11335..12141)
                     /locus_tag="OUD09_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT9692126.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            12307..13263
                     /locus_tag="OUD09_000840"
     CDS             12307..13263
                     /locus_tag="OUD09_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCT9692127.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(13340..13858)
                     /locus_tag="OUD09_000841"
     CDS             complement(13340..13858)
                     /locus_tag="OUD09_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCT9692128.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(13946..14095)
                     /gene="rpmG"
                     /locus_tag="OUD09_000842"
     CDS             complement(13946..14095)
                     /gene="rpmG"
                     /locus_tag="OUD09_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCT9692129.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            14323..14769
                     /locus_tag="OUD09_000843"
     CDS             14323..14769
                     /locus_tag="OUD09_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HCT9692130.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(14964..16859)
                     /gene="asnB"
                     /locus_tag="OUD09_000844"
     CDS             complement(14964..16859)
                     /gene="asnB"
                     /locus_tag="OUD09_000844"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HCT9692131.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(17184..>18139)
                     /gene="bsh"
                     /locus_tag="OUD09_000845"
     CDS             complement(17184..>18139)
                     /gene="bsh"
                     /locus_tag="OUD09_000845"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HCT9692132.1"
                     /translation="ATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKNLDKHYAM
                     IGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFIPWILGQC
                     STIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHLYDNPVGV
                     LTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDLSSVSRFV
                     KATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSCCNVDKGI
                     YYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
BASE COUNT         5475 a         3834 c         3107 g         5723 t
ORIGIN      
        1 aaaaagctgc ggtatcactc cattttccca cagcttttcc atcttttatt tcgttgattc
       61 tcgaataatc aactctgttt ccacaataat ctttcttgaa accggtgctt tggaattact
      121 taattgaaga atcgtctcca ctgctagctc tcccatccat tcggtataaa cttttacagt
      181 agaaagcgag ggcgttacat atttcgctac actgacatca ttgaagccca tcacagaaat
      241 atcttctggt accactaaac ctttttcttg aatcgctttc agtgcgccaa tcgctaatgc
      301 atcattagct gcaaaaagcg catcaggaac atgcggattc gtttttaaat actgcttcac
      361 tgcttgatat cctgactcga cagaaaaatc tgcttcgata attggacctt cgtcgtagcc
      421 tttttctgcc aaaacttgtt gaaatgccat aattctggga tcttttagcc gctgatggtt
      481 tttcttcgta tattctgttc cagcaagcat agcgatatgt tgcgctttat gggagacaaa
      541 gtgttcaatg actttttgaa cacttgactt aaagtcgaca actagagagt cgattccttg
      601 agaagtgccg tcagaatcta ccaaaagcaa ggcattttgg gatcctttca tttctaataa
      661 ctgagcttcg tcgaattttc ctagtgcgat cgttccatct gccggaagat cactaatgga
      721 atcccacgtt tctttcaata aagtaacgtt tgcttcttct gccttttttt cgattcctag
      781 gcgaatagat aaataataaa gatcttcaag ctcttccgta tcgtcgtacc actggatcag
      841 gcgaagcgtt tgcttagtat ttgctgcatt tttacgatgt ttcgtataat ttaagcgttc
      901 tgctgcttca aaaatttttt gctttgtggc taagccaacg gaaagatcag gatcatagtt
      961 gagaactctt gaaacagtag caggagaaac tcctgccaac tgcgcaatgt ctttaattgt
     1021 tgccatttgt ctctcctcct tttttacgtt tataaaatcg cagtaaatcg ttcaaacgct
     1081 gcctgtcctt tttcatctcg cttgaatact ccagcgtctt ccagtacacg tgcaaaaacc
     1141 tgacctacag atttttgtac gacatcggtg accgtatctt tcgtcaaatc aggatgttct
     1201 ttttttaatt gttctgccca cggacgatga tattcagcca tctggttttc ttctcctaat
     1261 aagaaatgct ctacttctac tagttctggt ttcaaacgag gtggtaaaac agcaagcccc
     1321 attacttcga tcaacccaat gttctccttt ttgatatgtt ggacatcttt atgtggatga
     1381 aaaatcccat caggatgttc ttctgaaaca ttgttgtctc gaaggacaag gtccaactca
     1441 aagtaattgt cccttcttcg tgcaattggc gtaatcgtat ggtgtggtgt accatctgct
     1501 gaaaaagctc tgatagatac tgtttcatcc gaatatgctt tccatttttc caatactgag
     1561 ttagctgctt caataagcaa gtttttctct gttgattgca aacggatcac agacatcggc
     1621 catttcacga ctcctgcgat gacttgcgga taatcttcta gtttgataag tttttctatc
     1681 ggtgcttttg ccataggaaa ttcatgacgt cctgcctgat aatgatcatg agaaagaatc
     1741 gatcctccaa caattggtaa gtcagcattc gaaccaacaa agtaatgagg caagacttca
     1801 gtaatccgaa gcagtctagc aaatgtttcc tttgtgattt tcatcgggcg gtgttgttct
     1861 gataaaatga tcgaatgttc attgtaataa gcataagggg aatattggaa tccccagctt
     1921 tcaccgtcta gattcattcg aataatccgg tgattggtgc gggctggata gttcaagcga
     1981 cctctatatc cttcgttttc catgcaaagc atgcaggtag gatagtctac ttttaccgcc
     2041 tcgcgttctg ctgcgatttg ttttggatct ttttctggct tagataaatt gattgtgatt
     2101 tccagttcac catactccga ctctgccgga aatttgatgt tttttgcgat ggctcttgtt
     2161 ttgatgtagt catttttctt acttaaatta aagaaatact cagttgcttc ctctgggctt
     2221 ttcgaatagt gctgtgcaaa gaatgcattg acgactgaag gtggcggtgt gagaaaatcc
     2281 attaattgcg cttcaaaaat ctcttgttct gctggcaatt ctgaaagaac accattttct
     2341 cttgccttag cgacaagctg atccaataag tctacagatt cttccttaac tggtcttact
     2401 tcaatgcttt ctagagcatc ttctcctacc atccctaaaa gacgattctg taaatataag
     2461 cggtccattt ccatccatcc gccagcttcg atcgcaagtg tggcaaaatc acaaatcgtt
     2521 tgactgattg acatatctca ttcccctatt cttcataacc atttggatgg ctgacatgcc
     2581 aatcccatgc tgttttgata atcgctttga tatctgtgta ttcaggtttc cagcccagaa
     2641 tctctctcgc tttttcactt gaagcgacta atcggctagg atctccagct cttcttggtg
     2701 ctatttctgc tggaatttct tttcctgtca cttctctagc tgcttctagc atttccttga
     2761 ctgagtatcc tttgctgctg cctaaattga agaagttgct ttcatttcct tctttcaagt
     2821 attccaaagc taaaatatgt gcagcaatca aatcttctac ttgaacataa tctctgatac
     2881 atgttccatc tggtgtatca taatcgtctc cataaacagc caaggctttt ctttgaccaa
     2941 gagctacttg taaaatgatt gggacaagat gcgtttctgg tgtatgatct tctccgatag
     3001 atgcatctgc ttttgctccg gctacattga agtagcgcaa agcgacatag cgcatgccat
     3061 atgcttggtc acaccacttc atcatttttt ccatcatcaa tttactttca ccatacggat
     3121 ttttaggatt agctggtgta tcttctgtga ttggagactc tttcggctct ccataagttg
     3181 ctgcagtaga agagaatacg attttattca cgttgaactc gtgcatgact tcaagtaaga
     3241 tttgcatacc gtatacgttg ttgttgaaat acatcaatgg tttttcgaca gattctccta
     3301 ctaaagagct tgcagcaaaa tggattactc cttcaatcgg ttccttttca aatacggaac
     3361 gtagaaattc tttatcccgg atgtctcctt cataaaaatg agcatctgga tgtactgctt
     3421 gtttgtgacc tgtgagcaaa ttatctacca cagctacttg ataccccttt tgaactagct
     3481 ggtctacagc gtgcgaaccg atataaccgg ctccgcctag tactaaaatt gtcataaaaa
     3541 acactccttt gattagtaaa attgtgtttg ttgattctat ataatttaaa aaacgaagtt
     3601 tacttttatg gtgaaaacaa acaaagtaaa atttcgtaaa ttcatatggt aaatttataa
     3661 taaaacattt agtaaacatt ttcaaggatt ttttgcaaag gtttacaaac aaacgtaaaa
     3721 ctagcctttt tttctctttt gttccataat agatataaca aataataaat gaggtggatt
     3781 caatgaaaaa atttgtatct ggaatattag taggaagtct ggcaacagcc gctgccgttg
     3841 ctggtttggt tgcttctgtg aaaaaaacag tgatcgatcc aattgacgaa aaagaagcaa
     3901 tgatcgaaga aaaccgtaaa aaagcaatgc gtaaacgcgt ttctcgttaa taattattta
     3961 aaaatacgca tgaagacttt ctaaaaaaca gataagtcat catgcgtatt tttatttgtc
     4021 agtcaatata agttgtgcta ttttatctgc ctgtctcaga ttccatggat ctgtataagg
     4081 tctagctact gctgcagtta gccaatccac accttcaaca ggtattccat agcaataaaa
     4141 gtcagactgg atttcccctg aagcatcaat cacgcgattc gtacctcgct caacatctat
     4201 agcttctgaa tgataacttc ctgtattatc tgaaaatgaa aaagggcgaa caagtttgtt
     4261 ttccttcaaa ttttttatga caggattact tgaacgagaa aagcttgttt gtggcaatcg
     4321 agcttcaatc aaatagcgac tgctgatttc tttatcttgt agagaaataa catagcggtt
     4381 gtgcttttgc tgaatatcaa atggcggttc gatcaaatga aaaatacccg catctatcag
     4441 tgctgctaat tctttcgtcc gaatgattgg cggaccgatc gttaaaaagc tatttagatg
     4501 agtaaaccat ccccatagta tttctttgta ttccttagca gaaaaaacgt gccattcaat
     4561 agcctgccgg attggatcac gccagtcttt taatgcatca aatgttgaag caaatgctcc
     4621 tgtgcaattg cctttttccg cttcttttat ctgcccttca ataaatattc ggctagcttc
     4681 tgaaaagggc tcattctgat aggcaagagg agtttctaaa tacgaccaat cccatctata
     4741 agggattagt gctggatgct ttccgagaca ctcctctgaa gagagggaga gaaaatcatg
     4801 ctcaaaattt ttctttggaa tcggcaagtg atattcttcg atgattttct tgtaataaaa
     4861 caactcaacg tcttttttca gatagtcaaa aaatgtttcg ccagtcaaaa atcccgactg
     4921 atgccattgc tccaggtttt cttttgttag taaccgtggc catgccattg ctccgccttg
     4981 cttttgattt cttcctcgag ggtaataagg gagccccctt cttgatccac tgtatacaat
     5041 cggttccttg cctgatggat gatagaccag ttttcccttt tcgttctcga agcgccctcc
     5101 tcttgatact gtaaacaatc cgacataatc aaaaaaactt aagcctagac ctcttaaaaa
     5161 gacattttct ttaggcggta tcttcgaaag atctacatct gcaggattag aaggaagctg
     5221 atacagtaaa acatttttct ttgcgtagtc tacccacact ctttcttctt ctgtctcatc
     5281 attttcccag tgaccgcttg ctaaaacaag ctgatcgata gaatagcttt gctgatctgt
     5341 ctgtaaaaga taaccatctt ttcttttgct tacttttttt actaaagaat aaacaaactg
     5401 aatagaagct ggaaattctt cttgcaactt tgagaaaaac cagcgttgat agagtccgta
     5461 taaacatcga gtagattgct gatctttttc cagacgattg atttcttcta taattagttg
     5521 cttatttttg gttaccgtct gttcgatata atctgtagca ggtcctttac tccattgaaa
     5581 caagttgggt ccctgtgtta ccaatcctcc actggacaat gtttcatctg taaaaagcgt
     5641 cacttgttga gaaacagaat tcattaataa ctccggtggc tgattcaacc gccaaactcg
     5701 acctccagga ccattgggat cgaaaagaaa aagttcaaga gaatgtctgt cctgattatt
     5761 atggagaatc ctttctgcta cacttaatcc tctaggtccg gcaccaatga tccctatttt
     5821 catttgcatg cctccatttc gataaatttt ttcaataaaa cagcatggtt ctgatgttca
     5881 ttttttgcac cataaacaaa agtaatgact ggatgttctt tcataagttg actgagttta
     5941 aaaatagctt gcttcttttc ttcttcgaaa atcaattctt gctggtattt ctttgcaaat
     6001 gaagcaaatc gttctggtac atggtcaaaa gttttgcgtg cttcattact aggtgcaatt
     6061 tctttcaacc acagatctaa ctgcgctttt tcttttgaaa ttcctcgtgg ccagaatcta
     6121 tccacgagta ttctatagcc atcctctttt tcagcatcaa ggtaaacacg ttttaatttg
     6181 atcatagttg tttccttctt tctacctttt cgcttagctt atatatgtag ataatagcac
     6241 aaaataaaaa aaatgaacgg cacaactatc cattgcgccg ttcatgtgat cttggtcaaa
     6301 atctaatgat tgattcggtt tttaaagctg tcatacaatc atctgtttta tactttttta
     6361 cttttgattt ttccagacca tccgtcatat ccacccttaa gtaaataaat ctcttgataa
     6421 cctgctttac gtaatttatt tgctgtacgg atgctcaaac ttttcttttg atcgtaaata
     6481 tagacaggct ggtcttttcg aatagatcct aatgaatctt tcaaaacagt gtaagggatg
     6541 tttcttgcac ctaaaatatg cccagcatca aacacatctt tttcgcgaac atcgattact
     6601 tgtgcttttc tcattccttc tcgaaattct tcttctgtca aagtcgttgc tgaacgtttg
     6661 gccattactc gtagatagac ttcccaaagg atgattgcaa gcacaatcaa tagcaaaatg
     6721 atattaatta cccataaaac catgtaatgc ctccgttttt ttaagcaaat ttcaaggcta
     6781 atgaggcagc accaatgaca ccagcttcat taccaagctc tgctaatttg atttttgttg
     6841 attctcttac ttgcgggaaa gtaaattctt taaaatattt ctctacacgg ctacgtaaaa
     6901 attctcctgc tgccgatact cctccaccta acacgatact tgacggattt aatgtattcc
     6961 ccaagtttcc gcaagctaaa cctaagtaga agcatacgcg atcaacaacc atcaatgcaa
     7021 acggatcgtc ttcttgagcc atttcaaaga tatctttact tgttacttct tctccgttgt
     7081 ctagcattgc tttcagtttt gaatcccctg cgtattcttc tgccaagaaa cgacctaggc
     7141 gtaccacacc tgtagcgcta gatacagttt ctaaacatcc gcgtttaccg caagtacatt
     7201 cgaacccttc aggatccaca gtgatatgtc cgatttctcc tccagctccg ctcacgccgt
     7261 gaatcaaatt tccgccagca acgatacctc cgccaacacc tgttcctaga gtaacgaata
     7321 caacgtctgg atcgttttct ccagcacctt tccatcgttc acctaaagca gcaacattcg
     7381 cgtcattatc gatagcgaat ttgattcctg ttcctgcctc gatcagtttt ttcacaaatt
     7441 gaacttctgt ccagtttaag ttatacgcac caataaccgt tccagcttcg ctgtccacgc
     7501 ttcctggggt acccatgccg attccgataa agtcttcggc tttcatatta tataaattca
     7561 aacgatgatt gattgattca atgatttcag gaacgatatg cttaccatca tccaaaatat
     7621 tggtatcgat gctccatttt tgctgaatct ctccttctgg tgttaaaatt gcaaattttg
     7681 ctgttgttcc acctaaatca atcccaataa tcttcttgtc cattacaaat ttcctttctt
     7741 tcggctttct tcaattcggt gctctctttt taatatgcgc cacgctgtca tgtatgtatc
     7801 atgatcaatc aaacggctat catacagatt acgcaattcg atagtcatta attcaatgtc
     7861 atagatgcgt gcacccacat agataaagat gccaaatttt ttgaacagct gctgtacatc
     7921 atataagctc tccatcttta agcacttcct tcatattata caagtaaacc atattttttt
     7981 aaacccagta aaatacatat tacaaaaaga aatacaaaaa ctagtgtaga aagaatcctt
     8041 tggtgtatcg aatagcttgt ctgttgtttc ggtagaccca aaacattccc taacactaat
     8101 ccgccaatta gcccaccgac atgtccccaa acatctacag aggtatccag aagaccaaaa
     8161 acaaatgtca acacaataaa taaaagaaaa tgtctgacta gctgctggat cgctgggtta
     8221 tgtttaaaat gaaaccctaa gataaacaac gcaccgaaca aaccgaatat cgaagtactt
     8281 gcaccaccag acagaacatt cgcttcgtta aaagcaaagc ttgctgtatt tcccattatc
     8341 ccgctgaaca tataaatcaa gaaaaaacgc caatggccat agattgcctc gatttgctgc
     8401 cccataaaat acagtatcag tgagttcatt acgaaatgca tcagtccaaa atggatgaag
     8461 attggtgtca ctaaacgcca ccattcattg aagtgaacaa gataaggacc atacattccg
     8521 gcataataag ggatctcaag tcgcggaaat aattccatca tgaggaagac cgctgtctgt
     8581 atccctaaaa aagcataggt ccataacggt cgtcttaacc atgcacgcaa ttttatttgc
     8641 tgttgataat tcatgaaaac ctcccatttc attttagtca ataaacaatt gttggatctt
     8701 ctgatcaaag ctctccggtt gccattgttc attcaattgt tcacgaaaaa caaggctaca
     8761 tgttttttta tgaaaattat ttaaataccg gtcgtaataa ccaccgccaa aaccgattcg
     8821 ataaccggct ctattgtaga taatacctgg tacaatgagg agatcgatca tctccggcaa
     8881 atagagcttc tctgattgtg gctcaagaac accaaaactc gaacgtacaa gaacggactg
     8941 ttcatccatt tcataaaacg ccatctgacg tttcggcaaa gttttaggta caactatttt
     9001 cttttgttct ttttctgctc gttggataac cggctgtgta tccagttcaa attcattcga
     9061 taaagtcgtc cctatcactt ttgctgactt ccagtactcg ctggcgaata gctgtttata
     9121 gattcgctgt tctttttcct ttttcttgcc acttttggcc agatttttca gcttttcgat
     9181 tcctttccgg cgtaatctta ctttcaattg gtccaaaaac tcacccttcc ttctctcata
     9241 aaatggtata ggaaaaaaag aaaaatagca atcgaacttt caaataaatg cataaataga
     9301 ataaaaagaa gacctagaaa tgattggcct tcctttttta atgaatttca actgttcagc
     9361 gcgtgttttt gtctgataaa aagtgcatac agaaaatatg gcgcaccaaa aagggcaatc
     9421 aaaattcctg taggtacttc cccacttagt agaatcactc tagcaaccat atctgcacta
     9481 agcagaaaaa tggaaccgaa caaaccgctc agaacaaagg tccagttatt ttctttacgt
     9541 acgactagct tcgcagcatg aggagctagt agtcctacaa aagataaact acctgtcacc
     9601 gctacactac cgcttgccaa agcaacagct aacaacaaaa ggattttttg acttttagcc
     9661 acgttaaggc ctaatccttg cgcgcgttct tgaccaaatg atagcaatac aatcctctgt
     9721 gcataaagtc ctcctagcgg aattaagata accgcccatg gaagcaatgc ccaaacataa
     9781 ggccagctcg caccccagat tgatcccgct aaccatgcat tcacaaatcc ataattttcc
     9841 ttagataatt ggatcgtgca tagcaatgtt aatgcagaaa ggccggcatt aacggcaata
     9901 ccggacaata agattcgatt catacccaaa aagccatttc gatcataggc cgcaagatag
     9961 acacagcaag cagctaaaac accacctaga aaggctacaa ctagcaatag aaacggcagc
    10021 gaatagtgcg aaaagaaacc taaataaagc atcactaaaa atcctgctcc ggcattaatg
    10081 cccaatatac tagaatctgc caattcattt cttgttactc cttgcaagat ataaccagaa
    10141 acgccaagac aagctcctgc taataaactg atcagtaatc gaggaactcg aaattctcta
    10201 acaatcagcg ccaaagaaga tgattctttc cctatgaacg tggcaaaaag ctgatccagg
    10261 taaataaaag gcgtaccaat cattaaacta cctatagaca ggatgacaag tacaaaaagc
    10321 aagaaataga acgtccaata tatttttttc accgttgttc cctccttact tgtatcagta
    10381 aaaacggaac gccaatcaat gcaatgacta atccaaaagg tgtttcaaaa gggggattga
    10441 tcatcctcgc tgtaaaatcc gccaacataa ccaatccacc gccaactaaa aaagagacag
    10501 gtagaacata acgatagtcc gttccaacca ttccgcgaac aactggcgga accaacagcc
    10561 caataaatga aacaggtccg actaacgaaa cggcaatggc tgctaacact gcaactagac
    10621 agaaaacaat caaccgcgtt cgtttaggat gacttccaag agctaatgca tgtgtttcac
    10681 tcaaatgaat caaagatatt gcttttccta atgaaaaaga aaccaaaaca gcaagtatat
    10741 ataaaggaaa caatactgct aattgtttcc atgtgatatt ggcagctccg cctacaaacc
    10801 aaaaagcaag atcctgctgc agattaaaga attgggtaag tgcttggctg atagcagtaa
    10861 aaaaagaact aatagctacg ccagctaaaa tcagctgtaa aggattcatg cccattttcg
    10921 aaaactgcaa aaaaccaaat acgagcaaag ttacagcaaa agaacctgcc aatgacatta
    10981 gaaacacaga aaaatcagaa gctttaggcc aaaaagcaaa actaagggcc attgccaacg
    11041 aagcacccgc gttgattcct agtaagcctg aatctgctaa aggatttctt gtcactcctt
    11101 gcatcaatgc accgctcaat gcaaaacaac tccctacaag aaatgctccc gccacacgag
    11161 gcaaacgaat atttcgtatg atttggtgac tttgttttga tctatcaaaa tcaaacaacg
    11221 cctgccaaaa atctgctttt cccacagtcg ctgcgccatt tgaaagagca acaaaaaaaa
    11281 taatcaaaag aacaaaacag accaaaagca ggagtctatt tttcttcata gtcctcataa
    11341 ttgatcctcc tgctcttttg gcaacaaatc ataggttaga aaaactggtt tatttgttct
    11401 tggaagcgtg acaatcgttg catcaatctc aaatattttt ttcatacact cttttgtgat
    11461 cacttcttca ggtgtacctt ctgtgatcaa cttaccgttt ttcattgcta ataaataatc
    11521 tgaaaaacgt gaagcatgat taatgtcatg aatcgacatc aagatcgttg tttgtttcat
    11581 ttgattgatt tcagtcaata actgtaggat ctctaattgg tgtgccggat ctaaaaatgt
    11641 tgtcggttca tccaagatca agatatctgt ttcctgagca agcgccatag cgatccagac
    11701 acgctgggtt tgtccaccag acagcgttgc gatatcccgc atacgaaatc cagctagacc
    11761 tgtagcatcc aacgcccact caattgcttc atgatcttgc gtgtccatgc cactgaacag
    11821 tttttgataa gggtatcgtc cgtaagatac tacttcttct actgtcatac ctgtagggtg
    11881 ttcactagtt tgtgctaata atgcaatttt ttttgcaagt tcttttgaac tgtatgtaga
    11941 gatatcagag ttattgacaa tgatctttcc tgatttcaca ggtagtatac gggtaaaact
    12001 tttcaataaa gtagattttc cacttccatt tggcccaatc aaactagtga ttttcccttc
    12061 aggaatcgta acagacatgt tttcaatgat ttttttgttt tcgtagccgg ctgcaatgtc
    12121 agctgctctt aatctcgtca tttcacccat cctttcgctg aaaatcattc tcaattatga
    12181 cgctgtttca acatttcgtc aagcgctact ttcatatcta tagccaaatg aagcggttga
    12241 cagcgttcga aatatcagga ataataacca atgagaaaga ttctcaatta tgttaggagg
    12301 aaagaaatga aattaaaaac aaaagtattc acattatcta gcatattgct gctggctgga
    12361 gtactgagcg cttgctcccc ctcttcagat aaagaaacag ctacaactgc caattcgacg
    12421 acttcatcca atagtactca tacagtcaca gatacacttg gacataaagt agagattcca
    12481 aacgcgccca aacggatcat cggcagttac ttagaagact atctgattgc actaggggaa
    12541 aaacctatcg cccagtggac tgttggcagt ggttccattc aagattatct gcaagatgat
    12601 ttaaaggatg tcccaacgat ttcttatgac cttccttatg agaaagtgtt aagctttgag
    12661 cctgatttgc tgttgatttc ttcttccgct actgtcgaag gcgggaaata cgaacaatat
    12721 agcaaaatcg cgccaactta tgttgtaaaa aacggaacag atgtcacatg ggaagaacaa
    12781 ctaaaagatg tcggcaaagc tttggcaaaa gaaaaagagg cagaaaaaat catttccgat
    12841 tatcaaaaat ccatcaagaa aactcgagaa gaactagcag ataaaatcga gaacaagaca
    12901 gctgcagtgt tgtgggtgac caacaactca gcctttatgg tcagcaaaaa tcggtcaagt
    12961 ggaagaatcg tatacgacga tctggaattt ggtgttcctg atctagtgga agaggtctcc
    13021 aaaaaagcca cttctgactg gtctgctgtt tcctctgaaa agctagctga attagatgct
    13081 gactacatca tccttgtgaa cagtgacaaa gatgctgcca tgttcaatga accaagctgg
    13141 aagaatctaa aagcagtgac agacaatcat gtactgcaat tcggacctga atctagttgg
    13201 ctatataacg gacctattgc cagtcaggaa atggtcaatg atatcaaaga aaaactaaat
    13261 tgattttaac taaaaaaagc atcttcacga ttagtcgtga gatgcttttt ttcttattaa
    13321 caaactagac gcatagattt tattcttctt ttgaagcaac cctatttttt actctttccc
    13381 atttttgaaa acgatgggca tatagatttt cttgatccgt tatcccctca tactcctcta
    13441 taagggcgaa gtctgaaaaa tcaatattcc caatatacgt atcgccttca aacgtgctat
    13501 cgatcaatgt tcgccagatc cgaccagtgt aaggaagaag ttcttgaaaa atccgactac
    13561 cacccgaaac atagatatct tctgagatcg tttgactata cgacaaaagt tcatcgatcg
    13621 aatgcattac ttccgcattt tctttcgtgc gaaaatttgg ttgcgttgtc aacacgatga
    13681 tatggcgata aggaagtgaa agatccccca ttccttcata ggtctttctt cccatcacta
    13741 aaattcgatc cattgtgtgt tcgcgaaaga aacgcatatc atttggcaat cgccatggta
    13801 acaaaccatt tttgccgatc agaccatttt tatcttgtgc ccacatggaa ataaacatat
    13861 tatccgctcc ttatgatttt agatgttttg agataaaaaa agtctcaatt ctttgaccag
    13921 caaggaattg agaactttaa aaacattatt ttgtttcacg gtgcaaagta actttgcgtt
    13981 cgcgtgggca atatttttgt ttttccaaac gatcaggatt gttacgtttg tttttgcttg
    14041 ttagatagtt acgttcttta caagaagtac actctaaagt gatgtttacg cgcatgtttt
    14101 tccctcccat ttttacaaga atcctctaag atgactctca gacttgatta tcttaccatg
    14161 attttttcta gattgctagt gtttttcacg attttgttaa gtaaaactac ttgacgataa
    14221 atacaacggt tttacttctc aaacaaaatc tttttctttt tgtataaata cccttacact
    14281 tgtttatata gattatgttt tttaataaag ggggatacta atatgccatg gaacatgaag
    14341 gactttcctg catcaatgaa aaacttagat aaattgacaa gaaaaaaagc aatcgacatc
    14401 gcaaatgcat tattagatga agggtaccct gataaccgtg cgatcccaat tgccattgat
    14461 caagcaaaaa aatggtatga agatgctagt gaatcagaaa aacgaacttt cgaaaaagaa
    14521 aaaaatccaa gcaagacgga tgaacacgat acgaatcctc gtgctggaaa actgcttgat
    14581 tcagatgtga tcgtagaata tgaggaagaa caatggatcg tgaaatctaa aggggcaaaa
    14641 aaggcaagca accattttga tacaaaaaaa gaagcaatcg aaaaaggaaa gcaagtagca
    14701 caaaacaaag aatcaacttt gttcatttat aaaaaagatg ggacaaaaga aaaagaaatc
    14761 gattattgaa catatgtaaa ggggttgtga cgaaaatcgt gtcacaaccc ccttatgttc
    14821 gaaacaatgg tgttcctctg ccaactccga accataagtc ataatatcta taaaacggga
    14881 agagctgttt tttgaaattg tttcttatca ctcagtgtta aacggtagga gaaaacctct
    14941 tttttattga tatttgattt tcatcaaaca gtcacatagt cgattgcttc tgcttgcgga
    15001 atcgtttcat ctacaaagta gactttatac catgtcaaga aactgaagat tgtccagatt
    15061 tttctttgct cttctttacg tccttcgtga tggtcatcta gcaacttcat gattttttct
    15121 tgatcaaaaa attcttttgc aaaatctgct tcgaataatg ctttgacttg ttcgtagccg
    15181 tgttcttctt tcaaccaagc tttgattggc acagggaatc ctaatttcac acgattcgac
    15241 cattcttccg gaagatgttt gttcgcagct tgtctgaaaa tatcttttgt gttgttttga
    15301 ttcaataaaa acttacttgg tatttgttga gccaatttca tgacttcgat atccaataaa
    15361 ggtactcgga cttccaaaga acttgccata gaaagtttat ctgcctttaa caagatatct
    15421 tttggcatcc attgatgaag atcgacatat tgcatcttat tgacttcatc tttgattcct
    15481 tctgttttct cgtaatgaac agtcatgatt tcatccacag atggtgctgt ttgatatttt
    15541 ggtgttaaca cttctgatgc ttctgattct tcgaacactc gagcatgacc gataaaatat
    15601 tcttttgcag gagcaagtga ttcatataaa tggatccgtc catggaaatt tttcattttt
    15661 ccgattttac gtcctaaatt atagcgtgtt ccttttggta attttttcaa tccttgagca
    15721 atcacacgga tgaattttga attcgtatgg aatccatacg tttggtatcc cgcaaacagt
    15781 tcatccgccc cttctccaga ttggactacc cgcacacttt gtgaagcaag atttgctaaa
    15841 aagtacaaag gtacgcaaga aggatttgaa tcaggttcat ctaagtgata ctggatcaaa
    15901 gggaatgctt tgaacgacat gtctccgtcg atcactgctg ctgtattatc taagtccaat
    15961 agttcggtta gttttctggc ttcaatcgct tcattatagg ttttatcatc aaacccgatt
    16021 gagaaagaat gatctggttt caatacagag gttacataac tggaatcaac cccgctggat
    16081 aagaaagaac caacttctac atcactgacc gtatgtgctt caatagaagc ttgaacggtt
    16141 tcgtcgattt tttcgatcca ttcttgtcgg gaatgcgttt cttttgtttc aaaatctgca
    16201 tcccaatatt gctgaatgtc tatttttcca tctttatacg tataataatg gccttcaggc
    16261 aaacgataga catctttgaa gaatgtttcg ccgtttaatg gagaatattg gaaagtcata
    16321 taaggtttca acgcttcttt gttcaattct ttgttaaaat caggatgagg cagaaaactt
    16381 ttgatttctg aaccaaacat aaatgttccg ttcatttctg cataataata tggtttgatc
    16441 ccaaagtggt ctcttgcacc gaataactca ttcttttcat tatcccaaat agcaaaagca
    16501 aacattccgc ggatcttttg caataattct gttccccatt cttcgtaacc atgcaaaagg
    16561 acttctgtat ctgcatgtgt ttgaaaaacg tggccagctg caatcaattc ttctctcaaa
    16621 ggctggtaat tatagatttc gccattaaaa ataatgatct ttgtccgatc ttcattgtaa
    16681 ataggctgag taccgccctc taagtcaata atacttaatc ttctgaatcc taaagcaaca
    16741 tgtttatcga tatattctcc tgaactatta ggtccacggt gaacgattcg atccatcatt
    16801 gtattaataa ttgttttttt gttgtccttg tcgttaacaa aacctacaat accgcacatt
    16861 taactttcac atcctaatat aaaattactg tttatttcat tcatatgcat cttatcatag
    16921 aataaatagc ggacgaatat tttctctctt acaatttcgt cacgtgaaaa caaaagatga
    16981 aagtttctgt ttttacagaa aataaagtcc caatatctaa tttaccatgt tttctttttt
    17041 gaggctacct tattctcttc gtgttaaaaa aatattagtg gatgctattt ccaaaaaaac
    17101 cagcaaaaaa agacacgacc taaagatcgt gtctaattat gactgtatca gaaaatatga
    17161 taccaaacgg atctattcat taattaattg acatagttta tctgttgctc ttttatgact
    17221 ggataactga ccaattcatg acgatccaag tcttctttat tcatatttac agcagtaatt
    17281 tgactgtctt catacgtacg ataataataa atccctttat caacgttaca gcaagaagaa
    17341 taaatcgtat attcgtattt tctttctcca gtatcgcata ctcctttttg ttgctcgact
    17401 gagcctagta tatgaaaaaa ttgactgata ctttcagatt ctgaatcacc tgatgcagca
    17461 ttcattttag taaatgtggc ttttacaaat cgagaaacag aagagagatc tccaggtaag
    17521 cccaatcctc ccatcccacg actataaaca ttcaagctga tctggtttga aaaatgattt
    17581 tctggtgttc catttgatag cgagcgataa ttgttcagat taaataattg ataatcaaat
    17641 ggcggattat ttgtcaacac accgacagga ttatcatata aatgaagtcc atctttcata
    17701 ctttcaatta ctatcgattt ttctttatca gctaataacc agtgtaaagg tgacaagggt
    17761 agtttttcac taaaatcgat attgactaga ttgatagtag ccaataattc tttagcttcg
    17821 tctatggttg agcattgccc tagtatccat ggaataaact caaatggtgc aacgttatct
    17881 tttccttctt gtaattcttt ataatcagca ttgccaggaa aattcagtcc tgccatactc
    17941 aacccttttt catttgtagc ctcataatag agtggatagt tggatacccc agctgctatt
    18001 cctatcattg cataatgttt atctaaattt tttacctttc taaaatcgaa acgataattt
    18061 cttggagtaa tagtgaccac ttcattataa gacatctcat aatcaaaatt ccttccaaaa
    18121 taatggtctt tcgttgcat
//
LOCUS       DAKBVG010000033        18091 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000033 DAKBVG010000000
VERSION     DAKBVG010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 18091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.033"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            72..2132
                     /locus_tag="OUD09_000846"
     CDS             72..2132
                     /locus_tag="OUD09_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HCT9692133.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2215..2784
                     /locus_tag="OUD09_000847"
     CDS             2215..2784
                     /locus_tag="OUD09_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCT9692134.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(2959..3765)
                     /locus_tag="OUD09_000848"
     CDS             complement(2959..3765)
                     /locus_tag="OUD09_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9692135.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3903..5417
                     /locus_tag="OUD09_000849"
     CDS             3903..5417
                     /locus_tag="OUD09_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HCT9692136.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5820..6803)
                     /locus_tag="OUD09_000850"
     CDS             complement(5820..6803)
                     /locus_tag="OUD09_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HCT9692137.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6808..7455)
                     /locus_tag="OUD09_000851"
     CDS             complement(6808..7455)
                     /locus_tag="OUD09_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCT9692138.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7540..8172)
                     /locus_tag="OUD09_000852"
     CDS             complement(7540..8172)
                     /locus_tag="OUD09_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCT9692139.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8188..8739)
                     /gene="coaC"
                     /locus_tag="OUD09_000853"
     CDS             complement(8188..8739)
                     /gene="coaC"
                     /locus_tag="OUD09_000853"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HCT9692140.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNTITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8736..9473)
                     /locus_tag="OUD09_000854"
     CDS             complement(8736..9473)
                     /locus_tag="OUD09_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HCT9692141.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9470..10459)
                     /locus_tag="OUD09_000855"
     CDS             complement(9470..10459)
                     /locus_tag="OUD09_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCT9692142.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10717..11259
                     /locus_tag="OUD09_000856"
     CDS             10717..11259
                     /locus_tag="OUD09_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCT9692143.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11276..12361
                     /locus_tag="OUD09_000857"
     CDS             11276..12361
                     /locus_tag="OUD09_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT9692144.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12373..13179
                     /locus_tag="OUD09_000858"
     CDS             12373..13179
                     /locus_tag="OUD09_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT9692145.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13180..14007
                     /locus_tag="OUD09_000859"
     CDS             13180..14007
                     /locus_tag="OUD09_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT9692146.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14004..15077
                     /locus_tag="OUD09_000860"
     CDS             14004..15077
                     /locus_tag="OUD09_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCT9692147.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            15505..>18091
                     /locus_tag="OUD09_000861"
     CDS             15505..>18091
                     /locus_tag="OUD09_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCT9692148.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFY"
BASE COUNT         5837 a         3210 c         3705 g         5339 t
ORIGIN      
        1 ttatataatg aaatcatctt atgaaaatta ctacttcatg tttagacaag agtagggagg
       61 actaagaata aatgtgttca aaaagacgtt caaaacaact aaataaacac gcttgtcgtt
      121 tagataagga acggtcgtgt tctgaaaaac actacgttaa gtttatgatg cgcacgatgc
      181 tgatcagttt atctgtaggt gtctatatgc atgtacccgt tcaggcaacg acacaaacca
      241 tcaaggtcgt acgtacctct gtttctgaga ccactgattc ccctatcact attactcata
      301 ctttcactga tgcaaatgga aaacagctac ttcctgcatt gacttcctat aataaagtca
      361 gttatcctcc aacgataaac cactactggt taattcctaa caaaacatta tggacatcgg
      421 aaaccggaag cagtgtgccg ctttctatct tttccgatat gactgatcct acaaaaggtg
      481 atcagttaaa acagatcact gatattttga atcatgcttt gacagatata aaggacggtg
      541 gaagcgcaca agtttcttat gtatatgaaa aagattattc acagtttgaa agaaagaaaa
      601 tcgatgttct ggcagggaca aaagtttcga tatctgatct ggtagaaagg ctgaagaacg
      661 cagatggaac acaaggagat atgtccaaag taatttcaaa caatgggaat atcgtggata
      721 cgactaatac aggaacatct actatttcct tgtcctattt tgatccaatt gctttaaaaa
      781 caatgactgc atctgctgtg atccatgttc gtgctgatca aacagctatc aaaggaaaag
      841 atcttcactt aagaacagga gagagttacg cccctaaaga tcttgttgaa tatgtaacaa
      901 aaagcgatgg aacttcagga aataaagaag aaatcaaaat gaacgggaca gtagatacta
      961 caactccggg agaatatccc gttgtattaa cttatataga ctcgaaaaca tcaaatgtgg
     1021 cagaaacaac agctgtagtt tatgtagaag catctgctgc ttcatcgact gtcatcactc
     1081 attttataga tagtcaaaca aataaagaac ttcaagcaga aacacatatg agtagtgtaa
     1141 atcgtattcc tgatttgcgc ctacctgctg aattttctga tcgagtgcca aatatagatt
     1201 tatcgactta tgaacaaaat gggaaagtca cttctatttc tgattggatg caagcaaacg
     1261 gattagacag aaaaagtcac tggtcggaga tccttgaagt acttcactca gaactgatga
     1321 ataataggaa gcaatcagga ggggaaatta cctttactta tgtttatata ccagacaatc
     1381 gttcattaga aggtcatgat cttattgttc atccaaacga aaaaataaac aaagaagagt
     1441 tgatccaatc agcaacaaat tccttaggta aacccgtacc gttaaaggat gtatcggtta
     1501 caatcaatga agtaccattg gaggatggtt atttgaccaa acaaacaggt gattttacga
     1561 ttatgtatag ttatcatgac ccgtattcac tccaagaact ccatgctcag tcactattac
     1621 atgttttacc attaaacaaa cagatagatc aaaaagaaca aaaaagacaa gaagaaagct
     1681 tgtcagaaaa aaaacaacaa atggctcatg cagtttctcc aacagggaaa gtaaaacaaa
     1741 caagaagcct agagttagca agaatatcta gaaaaatctc agaatctaat aaaacgataa
     1801 ggcaagaagc tcatttggcc ccacaagttt ctggattgga aaatgaaatt ttagagccta
     1861 aaataaaaca acaatcagaa aaagaatttg ttgctaaaca gtcggaagat cagaaggaat
     1921 cagatgacca gaaggaaacg aagaaaaaac gcttgtcaac tattgattcc accgatgagc
     1981 atctgcagcc gcaagtacca cttcctagtc cgggaacacc cgcacctggt ggaggtattc
     2041 cttcttctgc tttaggagat ttcatgcgag gtatggcagg aacatgggta tacgctaatc
     2101 gaaatgaatt tgacgcagat gactggttct gatttatatc tctatctgtt atagtcaaat
     2161 aagtagctga gtcatatgtt atttggcata atggagacga ggagggatag aaaaatgtta
     2221 gataaaacgt taccttacgc agaaatctgg atgacaagac cacttcaaca aaatgttcca
     2281 atcacgcctt tagcggaagg ttttgaattt cgattttatc aaaaagaaga tgaatctgct
     2341 tgggcggaga ttgagacttc cgttggggaa tttcaaacga cagatgaagc acttgagtat
     2401 tttcaacaaa actttgctcc ttatcctgaa gaacttgaaa gaaggatgtt ttttgtagag
     2461 acaaaaaaag gagaaaaagt agcaacctgt accgcttggc gaaaaaaatc aagagagcaa
     2521 gtgtatccgg tttttcattg gttagcagtg aaaccagatt accaaggaaa agggctcgca
     2581 aaagcattga ctgccagagt cttgcaagaa tttcctaccc tccatacgga cggaccaatc
     2641 tatcttcaca cacagacatg gagccatcag gcgattgctc tttataaaaa aatgggtttt
     2701 acttttattg ctgaaaatct agacggcaca cctaatccag attatgaaaa agttatgcag
     2761 gttttaagta aattagataa ataacgaaat ggtgaaataa gtcctcagta ttgggataaa
     2821 aaacgaaata gtcattcctc atatcttact tttttgagtt aatactatcg gataactaat
     2881 ggcatgccat acattcaaaa aagaaggggc agtgacaaaa gtattgtcac tgccccttct
     2941 cttgtttttt agcatgtttt atcgtttgat ttttttagga ataaaattct tgatgaacgt
     3001 aaatttttgg taatcgtttc gactatagag gtaaccaaat acactgaaaa caactgctcc
     3061 aataaaatta acaaataaat ccttcatagt atccatgatt cccacgtcta agtaaccatt
     3121 ttggatgatt gttttatctg ttttaccatc tgcattttga ctttcaatga ttgtccgagt
     3181 gatatctgga acatgataga cagtgtttcc gtctttactt aactccacac tattgatttg
     3241 ctggatgatc gtatcttttt gcatatcgaa ttgcagtaat ttatctgctg aaaattccac
     3301 aaactcccac ataacaccaa ctgtcatcga aaaacagaag gaaacaactg ctagtaataa
     3361 aggagtcaag ttcatggttt ttgtattttt attgagcaaa taaacaaggg aaaagccgac
     3421 acccccagct aaaaatccgc tgaatgcatg tagaaaagta tcccacattg aaaaatagcc
     3481 ataaaaatca cttaactctc ctaaaactgt tgaactaaaa ataaaagata acaaaatgat
     3541 ttctagcata tttggaatct gtatctttaa tatcttttcc agaaaataag ggatttcaaa
     3601 taaaattaat gtcagtaata aaagcattgt atttccccag tgttggttcc agacctgatc
     3661 cactagcata ataacgacga tgatacgtaa taaaatatag attccgtttg tgattctacc
     3721 tgcagttttt gtactgttcg taaaaaaggt tttcaaatga ttcataaaat aactccttca
     3781 tgtatatcct taattatact aagaagcgtg tgtttaccaa agctatatga cttaaagaaa
     3841 aacagagttg atattgtgaa aaaaatcact taatatagta taaggagaaa agggggatac
     3901 aaatgaaaaa attagttgct ggtttattaa cagccgttgc acttcttgct atgagcgcat
     3961 gcggcgcaga tcagaaaaat acagcgcaga caagttctag tacgaaggaa tctgccgaag
     4021 tatcatcagg cgcttctgaa caagtatata cagatccaaa agaaatgaaa gaatcttatg
     4081 atgtcgtgat tattggagct ggtggtgcag ggatggcagc agctatcgaa gcaaaagatg
     4141 ctgggttgaa tccagtaatt ttggaaaaga tgcctgttgc cggaggaaac acatctaaat
     4201 catcaagcgg tatgaatgct tcagaaacaa aatttcaaaa agaagaagga atcaaagact
     4261 cgaatgatgc cttctatgaa gaaacgctaa aaggtggtgg aggaacgaat gataaagaaa
     4321 tgcttcgata ctttgtcgat cactctgctg atgcggtcga ttggctagat caaaacggta
     4381 tcaaactgga caacttaacg atcactggag ggatgagtga aaagaggaca catcgtccgt
     4441 ctgatggctc ggctattggt ggatacttag tggaggggtt attgaataac gtccatgagc
     4501 gggaaatccc tatttttgtc aacgcagatg tgacagacat taagaaaaat ggggaaacgg
     4561 tcaatgaagt gactgtacat gttcaaggag aagaaccaaa agaggtcacc ggaaaagcaa
     4621 tcgtagttac cacaggcggt tttggtgcca gcaaagaatt tattgaaaaa tatcgtcccg
     4681 atttagctga ttatattacg acaaatcaag aaggatcaac tggagatggt atcaccatga
     4741 ttgaaaaatt aggtggacaa accgttgata tggataaaat acaaattcat ccaactgtgc
     4801 agcaggatga gggggtcttg attggtgagg ttgttcgcgg agaaggagca atcttggtcg
     4861 atcaagaggg gaaaagattt gtcaatgaaa tggatacaag ggataaagta tctgcagcaa
     4921 ttacagcatt gcctgaaaaa tcagcttatt tgatttttga ccaaggcgtg cgtgatcgtg
     4981 ccacagctat tgaattttat gaccaaaaag gatatgtcac aactggaaag acaatggaag
     5041 aattagcaga aaaaatcgat ttaccaaaag aaacactagc aaaaacagtt gatggatgga
     5101 atacggatgt tgcggagaaa aaagatgctc aattcaaccg tgccacagcc atggaacatg
     5161 acttatcgtc tccgaattat tatgcgatca aaatcgcacc aggaatccat tacacgatgg
     5221 gcggtgtgaa aatcaatacg aacactgaag tattagataa agaaaaccag ccaattaggg
     5281 ggttatatgc tgctggtgaa gtcacaggtg gcttacatgg aaacaaccgg atcggtggaa
     5341 attcagttgc ggagatcatt atttttgggc gacaagcggg catacaagca gctcgatttg
     5401 cttcggaaaa tgaatgaaat agaacgtaaa taaaaagagg ttgtgaaatc agcgttttga
     5461 gctaataccg agggtcagct gattgaacac tgtttagtga agaggttgtg aaaaagctgc
     5521 cctgcatcaa gtaataagaa cagccaagta aaaacagctt cttatgtttt taacttggtt
     5581 ggacttatta ccgcagatgc aagcttttga acaccgttta ttcggaaaaa gagcgtggga
     5641 caaaagtaaa gaacactttt gtctcacgct ctaaacacgt ataaacggcg gaagcagaag
     5701 aaattccttc ggaaataagc tgaaattcac aaaaatttga aaagcaattt tcgtgaattt
     5761 cttcttattt cttggagtta aacgcttctg tcccagcctc ttttttgatc agcatgattt
     5821 tattcagtta tcttcaaaat gatttttccg atgtttttgt ttgcttccat atattcatgc
     5881 gcttgtttga ctgcgctgaa ttggaatgta cggtcaatga ttggccgaat cttgccattt
     5941 tctagaaaag gcccagcttt ttgcataaaa gtttgtgcga gctctgcttt atactgatca
     6001 cttctaggtg tcaataatgt accaagcagt tggacacgtt tcccaatcaa tacaccaagg
     6061 tctatctcag gcacgatggt cccacctagc ataccaatca agacccaacg tccatcaacg
     6121 gctatagaag ctaaattctt ttcccagtaa gaagcgccga tgaaatccaa aataacatcg
     6181 actccccgac cttcagtgat ttttcgtacc tcttcttgaa agtcttgttt tttatagttg
     6241 atcaattcat ctgcaccaag ttctttacaa aaggcgagtt tttcttctga tcctgcagta
     6301 acgatgattt tcgcgtgaga aagctgtcta gctaattgga tcgctgctgt tcccacacca
     6361 cttgctcctg catggatcaa gacagtttcg ccttcttgta attttccaag ccagtaaagt
     6421 gtttgataag cagtcaaaaa aacttctgag attccagcag cagaaacata gtctagcgta
     6481 tcagggagaa ggattgcacg atcggcaggc atcacagcat attcggcata accacctaga
     6541 ttgactaatc cgtatacttt tgtcccagga gtaaaagtgg agatatcact tttattctca
     6601 actacctctc cagctacttc gactcccaaa atagggtagg gaggtttcaa agagactgat
     6661 tctcttttca taatatctgt tcgattgaca gctgctgtat ggactttgat cagtaattcg
     6721 ccttgttttg gtttaggaac aggcacttca atttgttcta atacatctac agaaccagga
     6781 tgcttgattg aaatggcacg catggatcta ttcctccttt gcccattctt ggaatgctgt
     6841 attcaactct ttgctacctt cagaaagtac tagagtcgta cgagaaatat ccgtttcttc
     6901 cactagagcg gcaagtacag cagcaactgt agagcgagtg atcgtttttg atgccttctc
     6961 attaggctta gttaattgaa tggaagtaac ttcttcatcg ttagtcaatg tgactggttg
     7021 aagaatgaca aaatctaagt tagttcgttt cagccattca tctgcataat gtttagtaat
     7081 ataataatct gtcatggact caggccattt tgctggttca tctgcataaa cagcactgac
     7141 catcagatag cgagagacat gcgcattttc agctgcgatc actgttttga ttgcgccatc
     7201 caagtctact tgcagaagat tctttccttg agagccggca gcaaaaatca atacatcgat
     7261 gcctttgaat gcttcggtca ttttttccac ggaccaagtt aaatcaaaag agacatacga
     7321 aacattctct ttttttacta atgtttggga agcaacatct ctcacccctg caaaaatttc
     7381 atgggaactt ggagctaaat cttttatcag gtgtcggcca atttgtccgt ttgcacctac
     7441 aacaaaaatt ttcatttgga tcatcctttc gacgtcaaat ctttccattc aatagtaaca
     7501 ccaaaagaga aaaaaatcag agatcggcta cgatgactat cagttagtaa gagaagggtg
     7561 cttgaagtat cttaacaaga cattgcatac tgctgcagtc gcaagacctg cgacgattcc
     7621 ttcaggtata ccacttgtaa aaataaccgc tagaatagct gaataaacag catttaaatt
     7681 tgttccgatg actttggcat aatcttgctg gaataaaaag tagatcatat tcatcactaa
     7741 aatcgtattg gtcaatgaac cggaaagtcc agctaagaat aaagaaacgg aatctgcttt
     7801 ttgtttcatt agtttgcgca gacctttata aacgaaataa ggaaccaccc caatcaagat
     7861 gcgaggaata aacgcgatca gcaaggcttt ccagcttcca tgttctgttc cgatgactgg
     7921 gataaacgga gaaaagacaa aagacaaagg tgtttggata actgtgctac ggatcatgct
     7981 gatcagtcca aatgtaccgc caagaaaagc acctagtctt ggtcctaaaa cgatcgaagc
     8041 aatgattaca ggaatatgca tggttgttgc attgattgga ccgataggga taaaacctaa
     8101 aggcgtaaca gctaataaaa tcaaaatagc taaaaacatc gcagttaatg taaagttttt
     8161 tgtatttttc ataagtactc cttttttcta agcatttctt tcattaagag tcagcatgac
     8221 ctgttcgatg atcgtctgaa tatcagctaa ggcacctttt ccaaaatctc cacaagctaa
     8281 aagagattct cttggttcaa tttcctgata gccgacagat ctcaatgttt ccagattacg
     8341 ctgagtgatc gtgttttgat acatattcgt gttcatggca ggggcaatca attttggtgt
     8401 tcccactgga agcgccattg cgactgttga aagaagatcg tctgcaatgc catttgctag
     8461 cttgccgata atgtttgctg tagcaggcgc gatgagaaac aagtcagctt gttttgctag
     8521 ttcgatatga ttgattacct gcggatttga ttcttgcatg acgtctgtat ggactgcgcg
     8581 cttcgacaga gattgcaagg ttaatggcgt gataaaatgt gtgctgcttt tagacataat
     8641 cacatctacc tgggcaccta atttgacgaa ttgacttgta agatcagcgc ttttgtaagc
     8701 agaaatactt ccagtaacgc ctagtaagat ctttttcatt ttgattcctc cagtctaatt
     8761 ttctcagtaa tcaatgcagc aatctcagat ttactttgag cttcttccac tgttccatct
     8821 ttagataaga gatagccgtg gtgtgtctcg ccatggattt ccgtcaaatc atttgcgaaa
     8881 ataaaatcag ttttgttatt tatcagacta ttttttgcga ctgccagaag agattccttt
     8941 ggtacatcta ctaatagttt gaaaccaatg atttttgctt ttggctgcca ttcacgtaga
     9001 taagaaatga tttttggtgt tttcttcaaa acgagcacaa gatgctcggt gttagaagag
     9061 atctttgaaa cagtggtatc tgtctgttca ttttccgcaa accaattgtc cagttcttct
     9121 tgcgttgaag aagtgggtag gttttttgcc agctgttctt ctgaaaaact aaaagcagga
     9181 gtaaaatcac tgactgccat actgtggata atcgcatcgt agtgttcttt tttcgttagt
     9241 tcttctaatt gtaaaaataa gtccagtgca gattcgatcg tataaatcgt tatattggag
     9301 tgtcttttcg gtttcagtgc ttgtttcgta gtcacgtaat cgattgtggt atccggattg
     9361 gcagctaaat aatcagctaa tgcttgccct aatcgtcctg tcgaatgatt tgtaatagca
     9421 cgtacttgat cgattttttc agtcgttcct ccagctgtga ttagaatctt catttatggt
     9481 cacctacatt tactagtgtt cttccttggt gtgttccagc aagtagttta gatgctgttt
     9541 caggcaattg atccagagta atctcttgga cgaccagttc atctgcaatc tgcaaatccg
     9601 ccatctgttg ccataaagaa agacgttgat ctattggtac attgacagaa tcaattccaa
     9661 tcaactggat gtttcgcaga ataaaaggca agacagtcgc gtttagtttg attcccccag
     9721 cattgccaca taaagctact gcaccgttat aagagatcaa tggaagaaga ctgcttagct
     9781 gctcgccacc tactgtgtcg atgacatagt cgatttgctg ttttcctaaa gcttttgttg
     9841 tctctggcag aaattcatca ggtcgtacga cttgcaaagc acctagactt tttagccatg
     9901 tgacagactc ttcttttctg gaaaaggcaa tgattgactg gaatcctagt ttatgtaaca
     9961 gggctatcgc tgtactgcct acaccgccag aagcaccagt aacgacaact tttttatcgg
    10021 aggtcatacc ttgacggatt aaagcgttca cacacaacat agcagtaaac cctgctgtac
    10081 caaggatcat cgcttgacgc agcgtcatat tttttggtag aggaactaac cagtctcctg
    10141 gtactttttg atattggcta taaccgcctg gatgggtgac gccaagccca aaaccagtag
    10201 caagtacaag atcttctttt gaaaaacgtg gattgttact ttggacgaca gttccggata
    10261 aatcaatccc tggagtcatc ggatatgttc gtatcacctg accagactcg ctcattgcca
    10321 atgcatcttt ataattgaca tcagaatagg caatttttac caagacttca ttttcagcca
    10381 actcagtaaa tggcagatca ctgattgctg tatggaaaac aggttccttt attacctgaa
    10441 aagatcgaaa agcgttcatt cgtgagacac tccttttaaa attttctttt atcataccat
    10501 aaagcctact cttttattct tgttcttccg taaatttaga caaaaattag gtgaaaaata
    10561 aagaatcaaa caactagaat ttattccaaa agataaagaa atattcagca aaaaaaattt
    10621 tacaaagggt tgcctttttc aataaatttc gtatactaat acgagtggta gtaaacagga
    10681 agtttactat tagaaaactt cgattcggag gaaattatgg aaattggtga gaagttaaga
    10741 aatttgcgca tccaaaagaa tttgacacaa gaagaactag gcgaacgcac agatttgtca
    10801 aaagggtata tttcccagtt ggaacgagat ttaagttcac catcaatgga aacattcttt
    10861 tctattcttg aagtcttagg tgtgactcca gaagaatttt ttcatcaaga agcagcgaat
    10921 ctgcaagtgg tttattcaaa agaagatcat acggtttatt tcgatgaaga aaacgggtat
    10981 gaattggaat ggctcgtggc tgattcgaat gaaaaagaaa tggagcctgt cttgctgact
    11041 tttgaaggtg caggagaata taagacgttt gaaccttcat tatctgaaac gtttattttt
    11101 gtattagaag gggaattaga gttaagctta ggagagacgg tctatgtggc aaaagcagga
    11161 cagtcgatct attatcaagc gacaaaacaa catcaattaa ggaatcatct caaaaaacga
    11221 acaaaagtat tgattgttgc aacgcaatca tatttgtaat atagggggta aaaacgtggg
    11281 gaaaccaatc atttcatttg atcaagtagt gaaacgctat gatgatgaaa cagtattaaa
    11341 aaaagtaagt tttgagatcg aacaaggaaa attttataca ctgcttggtc cttccggctg
    11401 tggtaaaaca acgatcctac gtattatagc tggattcacc gaagctacag aaggggacat
    11461 ctattttgaa gggaaacggt tgaatgattt gccagctaat aagcgacaag taaatacagt
    11521 cttccaagat tatgcacttt ttcctcatat gaatgttttt gataacgttg cttttggatt
    11581 gaagatcaag aaaatgtcca aatcagatat cgaaaagaag gtaaaagatg ctttgcgtat
    11641 ggtccagctt cctggatacg aaaaacggga gattagtgaa atgtctggtg gtcaaagaca
    11701 gcgtgtagcg attgcacgag caattgtaaa tgaaccgaaa gtattgctat tagatgaacc
    11761 gttatcggcg cttgatttga aactacgtac agatatgcag tatgaactgc gggatctgca
    11821 acaacgatta ggaatcacgt ttatttttgt tactcatgac caagaagaag cgttggcaat
    11881 gagtgatgag atttttgtca tgaataaagg gaaaatcgtc caaagtggta cacccgtaga
    11941 tatttatgat gagccaatca atcatttcgt tgctgatttt gttggggaga gcaatatcgt
    12001 ggacggtgta atgatcgaag ataatctagt tgagtttgtt ggtaagcaat ttgaatgtgt
    12061 cgatggcggg atgcgtctaa atgaaccagt tgaagttgtt ttacgcccag aggatttgac
    12121 gatcacgaca ccagataaag gaaaactagt cgtaacggtc gatactcagc tgtttcgtgg
    12181 tgtccattat gaaatcatct gttatgatga acagcaaaat gaatggatgg tccattcaac
    12241 gaaaaaagct aaagaaggaa gcaaagtagg attggccttt gagccagaag atatccatgt
    12301 catgcgtttc aacgagtctg aagaagaatt cgatgcacgt cttgacagct acgaagaata
    12361 ggggggacaa gaatgaaaac aatgcgcagg atctattccg tcccttatat tttgtggctc
    12421 ggtttattcg tgatcgcgcc tgtattaatg atcatttatc aatctttttt tgatatgaat
    12481 gggcaattca cgctgaataa ttatctatct tattttacgt cggggacatc gctttctatg
    12541 acgatcaatt ctgtatggta tgcatttttg atcacgtttt tcacgctgat catcagttac
    12601 ccgactgctt attttttaac aaaactgaag cacaagcaat tatggctgat gctgattatt
    12661 ttgcctacat gggtgaatct gctgcttaaa gcttacgcat ttattgggat tttcagtgtg
    12721 cacggcagcg tgaatcattt ctttgatttt atcgggatcg ggccgaaaca gatattattc
    12781 acagacttca gttttctgtt cgtggcaacg tatatcgaga ttccatttat gattatgcca
    12841 attttcaatg cattggaaga gatgaatcct tctttgatca gtgctagtag agatttaggc
    12901 gcgaatagct ttgagacctt ccgacgagtg atttttccgt tatcattaaa tggtgtgaaa
    12961 agtggtgtcc aagcagtatt tattccttct ctttctttat tcatgctgac tcgtctgatt
    13021 ggcggaaatc gggtgatcac tttaggaacg gctatcgaac agcatttctt ggttacacaa
    13081 aactggggga tgggttctac aatcggtgtg attttaatca ttgctatgtt catcgtgatg
    13141 ctcctgacag gcgagaagaa aaaaggaggt cgctcataaa tgaaaaaatt taaatggtcg
    13201 tatctttatt tagggatcgt ctttttattc ttatatgcac cgatcttcta tctgattttt
    13261 tattccttca atgaaggagg aacgatgaac agctttacag gatttacttg ggaaaattat
    13321 gaagctgttt ttgaagatac acgtttgatc attatcgtat tgaatacgtt catgctggct
    13381 ttcttatctg cattgatcgc cacgatcatc gggacatttg gggctatggg aatctattac
    13441 acgaaaaaac gacgtgcacg gacagcgttg ttgagcatga ataacatctt attagtctct
    13501 cctgatgtta ttatcggtgc tagtttcttg attttcttta cactactagg attcggatta
    13561 ggttttcaga gtgtattgct ttcccatatt gctttcagca ttccaatcgt cgtcttgatg
    13621 gtcttgccaa aattgcagga aatgaacgat tcaatggtcg atgcggctag agaccttggt
    13681 gctaataatt tccaagtagt caaaaatatc attttgcctt atctttctcc aggaatcata
    13741 gctggatatt ttatggcatt tacctattct cttgatgatt ttgcggttac atttttcgtc
    13801 acaggaaatg gctttacgac gctctcagta gagatctatt ctcgtgcaag acaaggaatc
    13861 agtttagaaa tcaatgcgct aagtgcgtta gttttcttgt tctccatgat attggtcatt
    13921 ggatactact ttattagtaa agaaaaccgt ccaaaacggc tgaagaaaaa cagaagagta
    13981 cgcgaggggg tggcacggct gcgatgaaaa aattacaatc tctgttttta ggtattctca
    14041 tcattattgc ggtgctgctg cttggtgttc gtcaacttga aaaaacaagt ggcatgagtg
    14101 gagcaaaggt acttaccatc tataactggg gagattatat cgatcctagt ttgatcacga
    14161 aatttgaaaa agaatatgga tacaaagtga attatgaaac atttgattct aatgaagcga
    14221 tgttcacaaa aatccagcaa ggtgggacaa actacgacat cgctatacca agtgaatata
    14281 tgatccaaaa aatgatcaaa gaaaaactag tacttccatt agatcattcg aagatcaaag
    14341 gattaaataa tattgatcca cgatttttag acttggattt tgatccagat aatcagtatt
    14401 cgattccata tttttgggga actttaggga tcgtatataa cgataaattc tttgatgcaa
    14461 atcaaattaa gcattgggat gatttgtgga agccggaatt aaaagacagt ttgatgctga
    14521 ttgacggtgc acgagaagtt atggggcttt ctttgaatag tttaggctat tctttgaata
    14581 gtaagaatat ggcacagctg acagaagcag caaataaatt gaatcattta acaccaaatg
    14641 tcaaagcaat cgtggcagat gagatcaaaa tgtatatgat caatgaagaa gcctccgtgg
    14701 cagtgacatt ttctggcgaa gctgccgata tgatgtggga aaatgaacat ttacattatg
    14761 tgatcccacc agaaggatcc aatctttggt ttgataatat cgttatgcca aaaaccgtga
    14821 aaaataaaga aggtgcctat gatttcatca actttatgct agaaccggaa aatgcggcac
    14881 aaaatgcaga gtatattggt tattctacac caaatgaaga agcaaaagcg ttattgccaa
    14941 aagacatttc gtcggacgaa caattctatc catctgatga cacgatctcc catcttgaag
    15001 tctacaaaga tctaggatcg aaatacctag gcatctataa tgacctattc ttagaattca
    15061 agatgtatag gagataatgg caaaactcag taagaatggg gacttatcaa cgttctaaag
    15121 agctaaaaat cgtccttttt ctatctgagt tttccaatct tctaccaaaa gcggatgaaa
    15181 cgccgctttt tttgtgttat tttatttttt ctaccacgtt tttctaccag agaaaagtgg
    15241 acaaaaccat tgtaaaatag ggcccgagac gggctagtaa atctaagatt gaaaaagtta
    15301 cagaaggata aagtaattgg aaactatgca aatatactta acccttctgt aatctattaa
    15361 taaaactaat atatgaatcc agttcaatat ttgtagctat gaaaaaacat aaaaatcata
    15421 ttagataaaa actaaattgg tagataaatt attttgtcag atatctgagg caaatattcg
    15481 actatttgtg tttcaaagta aaaattggaa tacaaatata gaatagacat attgcagccg
    15541 atagtttatt atgcttttag acacattaaa ctggaggaaa aaatgaaaaa gagcgctaaa
    15601 gatcgagttt ttgatgagct gatacgacta acagggaata tgtcttctgt cactgccgag
    15661 aaaatttcga agaatctagg tatctctcgt cagaatgcca gccattattt gacgagatta
    15721 gtggaagata aaaaggtcga gaaattaccg ggaaagcctg ttttgtggaa gcctttagat
    15781 gaatatgcgg ttgtagataa tacggagcaa ataaacgaag cctttcattc tgtagttggt
    15841 catgacggaa gtctgagaga ggtcattcaa aaatgtatag ccgctgtcaa atatccgcct
    15901 aacggactca gtgtattaat aaatggagcc acgggtgttg gaaaaagttt tttagccaca
    15961 aaaatttttg aatacgctat ccatgagcaa attattgaaa aagatgcacc atttgctatc
    16021 ttaaactgtg ccgattatgc ggacaatccc gaactgcttt cggcaacact gtttggatac
    16081 aagaaggggg cctttacggg ggctgaaaag gataccgatg gtttattggc aacggccaac
    16141 aacggctatc tttttctcga tgaggtccat cgcctatcga aagaaaatca agaaaagctc
    16201 tttctattta ttgatactgg aaactatcgt ccagtcggtg aaaatgtaaa ttggcattct
    16261 gctaaggttc gttttatttt cgccacgacg gaaaaaggtg aaaattattt attagacacc
    16321 tttgatcggc gtattcaaat tagtgttatg ttgccaactt ttgaagatcg ccccattcga
    16381 gaacgattag agctgattca attatttttt caaaatgaag cggatgtttt acaaaaagat
    16441 attattgttt caagagaagc cttgtcttta atgttatctc atccctttag tgggaatatt
    16501 ggtaagctga agaatatcat taaaatcagt tgtgcggatg tgtatagtcg aacacaagaa
    16561 gaaccattaa cgattgggaa aaatgagatc ttgattcagt taaatatgct ggaatccgaa
    16621 gtagaaagct tgccgattgc tagtttattg ataaagaaaa atcaaaagat aaaaacggat
    16681 cgaatcaaac cgtttgatta tgattcgatt ttggaaaaaa ttactcagct gataaatgaa
    16741 ggtaatttca atgaaaattt ttcttcgata aagaatgaag tacagcattt ggtgcatcac
    16801 attgataagg attctttttt atcaatgagt agaaaattga tgattcagat gtttagaaag
    16861 gtctgggtat ccgttttagg aaaaacgtac ggattgacga cgagtggacc tgttgcagaa
    16921 attattagca gatcctatgt catttcccca agctataaga cagacttaac cgggataaat
    16981 cgtacattag ctgcaaatct acctcggacg acttacttat gtaatcagtt cttggagcat
    17041 ttaccaccgc tctcagaaaa ggacgagaac tttctaagac tgcttattgt cgtgacttta
    17101 tctgattatg tagatgaatc catcgagctg aagggcctgt tgcttgctca cggagatagt
    17161 acggctagca gcattcaagc agtagtgaat caattatgcg gcaattatgt ttttgaagcg
    17221 ttggatatgc cagtagagag taatgtgtca gagattgtcg aatcgacaag gaatttcgtg
    17281 aaacggcaag tgaatacaga tagtctagtg ctgatcgtag atatgggctc tttaaatcaa
    17341 atttacacac agatcaagaa cgatttaaaa ggggagttac ttcttctgaa taatttgacg
    17401 acttccattg cacttgatat tggcttgaag atgacgatga atgcgccatt taaacagatt
    17461 gcggaggatg cagcagacaa gtattcaatc aatgttcaat actttgaagg cttttctcaa
    17521 agtagcaaca ttatcatttc ctgtatgtcg ggtttgggga tttcggacaa attgaaagat
    17581 gtttttcgtc atcatttatc ggatgaatcc atcgaagttt tcacgaagga ctacagagaa
    17641 ctacgtgaat taatcgacca aaatgatgaa acttattttg acaagacaaa attggtcatc
    17701 acgacttcgg atcttccacg ttccttttca attcctaata ttaatgttta tgacatctta
    17761 gacggtgatg gagcagccaa tttggagcat attttgagtc ctgaaatgaa gccggcagaa
    17821 tttaaccgct tgattcagga gctagtaaaa ttcttttcgt tggaaggaat tgcggaccgt
    17881 ttgaactttc tgaacccaac gatcgtgatg agtgaagtgg aaacagtcat ttctaaatat
    17941 gaaaattact atcatttcac tttaaatggc cgtgtaaaac taaatctcta tatgcatacg
    18001 gcattgatga tggaacgttt attcttggct cggacagaaa aatctgaaga accgaattat
    18061 gatttgaaac cagatgaaga tgaattttat t
//
LOCUS       DAKBVG010000034        18052 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000034 DAKBVG010000000
VERSION     DAKBVG010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 18052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18052
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.034"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            214..498
                     /locus_tag="OUD09_000862"
     CDS             214..498
                     /locus_tag="OUD09_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HCT9692149.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(779..1198)
                     /locus_tag="OUD09_000863"
     CDS             complement(779..1198)
                     /locus_tag="OUD09_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HCT9692150.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(1200..1784)
                     /locus_tag="OUD09_000864"
     CDS             complement(1200..1784)
                     /locus_tag="OUD09_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HCT9692151.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(1831..3306)
                     /locus_tag="OUD09_000865"
     CDS             complement(1831..3306)
                     /locus_tag="OUD09_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HCT9692152.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            3487..3798
                     /locus_tag="OUD09_000866"
     CDS             3487..3798
                     /locus_tag="OUD09_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9692153.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(3913..6249)
                     /locus_tag="OUD09_000867"
     CDS             complement(3913..6249)
                     /locus_tag="OUD09_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCT9692154.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            6391..7059
                     /locus_tag="OUD09_000868"
     CDS             6391..7059
                     /locus_tag="OUD09_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCT9692155.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            7062..8105
                     /locus_tag="OUD09_000869"
     CDS             7062..8105
                     /locus_tag="OUD09_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCT9692156.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(8342..9223)
                     /locus_tag="OUD09_000870"
     CDS             complement(8342..9223)
                     /locus_tag="OUD09_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCT9692157.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            9382..10110
                     /locus_tag="OUD09_000871"
     CDS             9382..10110
                     /locus_tag="OUD09_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCT9692158.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            10266..10583
                     /locus_tag="OUD09_000872"
     CDS             10266..10583
                     /locus_tag="OUD09_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCT9692159.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            10576..11184
                     /locus_tag="OUD09_000873"
     CDS             10576..11184
                     /locus_tag="OUD09_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HCT9692160.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDYGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            11239..12030
                     /locus_tag="OUD09_000874"
     CDS             11239..12030
                     /locus_tag="OUD09_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HCT9692161.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            12168..12680
                     /locus_tag="OUD09_000875"
     CDS             12168..12680
                     /locus_tag="OUD09_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HCT9692162.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KIKKVTLDIY"
     gene            12706..13578
                     /locus_tag="OUD09_000876"
     CDS             12706..13578
                     /locus_tag="OUD09_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HCT9692163.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            13754..14116
                     /locus_tag="OUD09_000877"
     CDS             13754..14116
                     /locus_tag="OUD09_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCT9692164.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            14125..14877
                     /locus_tag="OUD09_000878"
     CDS             14125..14877
                     /locus_tag="OUD09_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT9692165.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVKPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            14891..16693
                     /locus_tag="OUD09_000879"
     CDS             14891..16693
                     /locus_tag="OUD09_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT9692166.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYRVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAEKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            16970..18010
                     /locus_tag="OUD09_000880"
     CDS             16970..18010
                     /locus_tag="OUD09_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HCT9692167.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
BASE COUNT         5662 a         3137 c         3729 g         5524 t
ORIGIN      
        1 aggaagaggt tgtgagcctg ccgtttaact ctgagtcaca aggagcaatt tcaaaaaaca
       61 gcttctcatg tttgttgact attgtgactt gtgaccgagg agttggcagg agaacaccgt
      121 ttattcgaaa aaaggaactg cgacaagact tttgtcacag ttccttcttt tttttgctat
      181 gctatactag atgtaaaaaa taggagaaat aaaatgaggg aaatttattc ttttgaatca
      241 aaaatctatg cttccgaagt cggcaagggc ggggcatata ttatttttcc gtatgatata
      301 agaaaagaat tcggtgctgg aagagttaaa gtccaagcaa cttttgacaa tgtgccgtat
      361 gaaggcagta tcgtgaatat gggcgtgaaa aatccagatg gttcagtctg ctacatcctt
      421 ggactacgaa aagatattcg taaaaacatc ggaaaagaaa tcggtgatat tgtagcagtt
      481 acagtgaagc aaaaataaaa gaggttgtga caaaagacgt gtcacaacct cttgataaac
      541 ggtgttcaaa agccagtttc tcggtagtaa gtcaaattta tgcaaaaatg tggaaaccca
      601 tgtttacaaa atttttctta ctactcgaaa cgaagcggct ttatcacaac ctcttgataa
      661 acggcgttca atcagctgtc cctcggtatt agctcaaaaa cggcaaaata tgagaagcta
      721 tttttccatt tttttctctc atactcaggt cataacgctg atttcacaac ctcttctttc
      781 ataatggttt ttcaccatct ttataataaa ctggttcttt gatatggaaa tctatttttt
      841 ccaaccaatc taaaatcttg taacctaact cttctgacat cggaaaagag gcgttgattt
      901 gtgcattgga gatatggtca acatggatac cggaagtgat cgtacaactt cccggaacgg
      961 cagttttgat gatcacagct attttttcgg ctaagacatc gtctttatgg aaccgaccat
     1021 cgtggctagg gaaacgtaca gtttgcatct ctgtatcacg agaaaatgta gtgactgttc
     1081 cgatatgtgg tgtatctccg cctgttaaag tgatcaataa gtctttaccg attactttaa
     1141 cagcagcttg gatcgtgaaa cctagtttgg ttacttcaac tatcgtttct ttttccatat
     1201 taatcaccct gccaacgttt tgaatgctca tttacaattt caaattgatc caaaagtttc
     1261 attgtatggt gattaacaag atcttggatc gtttgaggct gattataaaa agcaggaatt
     1321 ggtggtatga tttgcacacc tagtcgtgct aatttagtca agttttccaa gtgaatggca
     1381 tttaatggcg tttcacgagg aacaatgact aactttcgtt gctccttcat tgttacatca
     1441 gcagcacgtg caatcaggtt gtcgcctaaa ccaacagaga tacctgcgac tgttttcata
     1501 cttgccggta cgatgatcat tccatcggtc aaaaatgatc cgctggcgac agcagcacct
     1561 aaatctttgt ttgaataatg gtaatcaaac aggctttcga attcagttag tgataagtcg
     1621 gattccagct ttaagttctg ttttgcccat ggactgatga ttccgtgtgt ttctatcatc
     1681 ggatattctt ttagtttctt cactaggttg atggcataaa tggtgcctga agcgccactt
     1741 acaccaatca cgattttttt cttccgttgt gccaattgtt ccattttccg cactcctttt
     1801 tatgatgtca aaacagttgc agatcattca ttatttcaaa tatttttccc aatcttctac
     1861 ttcttggaat tttgcacgta caaaatgttc tttcatatcg aaaggaactg taccgtcaaa
     1921 gatagttttt gcacttatcc cacggaaacg aatagagcca ggattatatt ctggtaattc
     1981 tgatgggtcc aatggatggt tcctcattcc tgggatcacg atgatatctt tatctccttg
     2041 gaaacgagtg ttgaaagtcc aaactacatc gttcatgtcg aaaatatcaa catcttcatc
     2101 gactaaaatg actgtcttca attctttgaa ggctgataaa gcaagaatag ctgcttgacg
     2161 ctgaatacct tcatccgctt cgttttcttt acggatttgc atgatggtca tcaatttacc
     2221 accacctgca ggaggattgt agacatttac tacttttcct ggaatagctt tatcgaccaa
     2281 ggcaagaata cttgcttctg taggaatacc ggccatagat acgtgttctt cactgggacc
     2341 aatcgttgtt tgcatgattg gattgtcttt tcggtgagta actgctttga ctttcactac
     2401 ttgtacagct ggattagcat ccccagtata gccaggaaat tctggcattg cttttcctga
     2461 gttcgtgttg atatcttctt gcattgtttc atttggcatg atttctgctt caatgatgaa
     2521 ttcagcacga gcaataccca cttcatcgac tgctatacca tcaacgattt gaataggttc
     2581 gttacgcaaa gctcctgcac accatagttc attgtagcca agtggagtgg taggtggttc
     2641 gaaagtcgtg ccaatggcaa ttgctggatc taaaccaata ttgatagtga taggcattgg
     2701 tttattcaag gcttcgaatt gtttttggaa gtgaccaatg tggcgtccgc caggcatgat
     2761 gtacataccg atcgtatctt tatcttctag aaccatacga tggatcgtta catcgctcat
     2821 tgttttttct ggatctgatc ctaagactac acccattgtg atgtatggac cagcatcttc
     2881 aggagtattt gtaggtgcag gcaaaatgtt gcgaatatcg aagtctgcat cggtagctag
     2941 atgaacgact tcttgtgctg gagcgtctgc tttgtctact ttcacaggtt tgattggatt
     3001 ttctacagcg tctttcaaga aacgaccaag tgttcgataa tcatggtgca aaatttcacc
     3061 tacacgttta cgactagcca ttgttccgat gttcacacgt gtgccgggga aacctttgat
     3121 attgttgaaa gtcatagctg gtccttcttg agtcggacgt tttactgtac cgccagcacc
     3181 gatataacgg taaacaccag ataattctgc ttcaggatcg atttcaatat ctgtttcgtg
     3241 atattgacct ggaagtgttt ttagttcttc gatgactttt cttaaatcat acggttgatc
     3301 tgtcatgaga gattcctcct tagttgtctt tacctatcta tagtaaaagg tgaaaaagag
     3361 gaaaacaatt caacttgttc ggtaaagtat tctacaatgg aatggatgcg tttttttgat
     3421 aatagaataa actatgatat acttatcact aaaagaaagc gcatacttag tgttggagga
     3481 aggaaagtga aaaaaatgga ggagtcattt cctaaagcag taaaagtgga aaatatagcg
     3541 aatattttga aagttacatt tgaaaacgga gaagtaaaat acgtgaaatc tcattggaca
     3601 gaagagataa ccgatgcctt gcaatttgga aaaaaaggaa gaggaaaaag aaaaaatctt
     3661 ctcgcacttt ctcgtaatat gtggattggg actgaagtaa cgattgaagc agatggaaca
     3721 gtattcataa atggaaaaga caggtatacg ccagaagagt tgtggtacaa aggaaagaaa
     3781 agtataccag aattataaaa aataattaaa aaaaacagcg cgaaacaagg aacatattac
     3841 ttcctcgttt cttgctgttt tctactattt cagcgctctt atgactcaaa tctaaatgtg
     3901 gttgtcacaa ccttattctg cacgcaaagc gatagcggcg tcttttttcg ctgccattcg
     3961 tgcaggaaga tgaccgccaa gcatcgtcaa tatagtagag acgatcacta gaataatcgc
     4021 atggacagga tttagttgag caacattttt tagatccgtc atgttgtata agatgctatt
     4081 gatagggaac gtagctagcc atgcaataaa cacacctaat atcccagaag aaatacctaa
     4141 aatacacgtt tctgcgtcaa atacgcgagt gatgtctttt ttacgtgcac ctaaagcttt
     4201 tagtacaccg atttcttttg tccgttcaat cacagatgta taagtaatga tactgatcat
     4261 gatcatactt gttactaaag agatccctgc aaaagcaata aggacataag tgatagcatc
     4321 catcaatccg ccagtcagtt cagtcatcgt tccagctaaa tcagtataaa tgattttatc
     4381 ttcatctgat ttgcctttat tgtaatcatc tagataatcc aagatctttt ctttgtcttc
     4441 aaaattgttc ggataaatca tgatacttga aggcaagctg tctccaccta aataactaag
     4501 caatgtttgt ttggcatttt cgtcaacttt ttcagtagtt aagacattta caccactgtc
     4561 tctttgagct ttgacgattt ctgattcttt gttttcgttg acgatttgag tcgttaattg
     4621 atcgctataa gcgattcctg gagataagag attcattgta gaagaagatt tcacgcgtag
     4681 gatccccgag atcttcaatt catcagaagc attctgatac atcgcatcat aatctgtatt
     4741 agggataaaa ttcccagttg gaagttttgt atagtaagta ttgttgttta ctaatttgaa
     4801 tgttgtacca acgatatcat caaaatcaag tttttcgtct tctttcacat caaatccaag
     4861 attcttcaat gcgttgatat tagtattatt attgccgtct acgatcagca cgacatctgt
     4921 tgctgaggct ggataacttc cggcaagtaa agaataattg tcttttaaaa agttttcttt
     4981 gctagtgtca agctgtgtag ggaaacttga cactcccaca cctgtcatcg ctgacatcgt
     5041 actagataaa gaaagagatt ccgtatcagg attttgattc gagaagctga caggctgtac
     5101 tttgccattt acatcacgta aaagattgat tcctgtagta cgggtaaatc cgatattatt
     5161 acttaagtta ggatcgatgt ctgttacgta atcgacgtat gtttgatcaa tcttattcgt
     5221 gtgctgtgct ttatcttctt cgctgacttt tgcagtaatc gttttgctgt ctggataatc
     5281 cgctttgctt gcacccaatc cggcatcgtc acgagtctgg ttagtcgtta ccttagaaat
     5341 agtaatcgga aatttcgcca tcgtttcagc ttgcgtgtta tcgatttgtt tttggaatcc
     5401 agaagaaagg gacagaacga tcgcgattcc gatgatccca atactggaag caaaggaggt
     5461 caagaaagta cgtccttttt ttgttcggat attgttgaag gaaagcttta acgctgtcca
     5521 aaaactcatc tttgttctgc gtaagttaaa ttgatcatct tttggtcgtt cgatgtgagg
     5581 atgggaatct gttaagatct taccatcaga aaattcaatg attcgatctg cgtattgatg
     5641 ggccagttca ggattatgtg tgaccatgat cactaatttt tctttcgata actcttggat
     5701 caattccatg atctggatac ttgtttctgt atctaacgct ccggttggtt catcgcacaa
     5761 aaggatatct gggtcattgg ctagtgctcg agcgatggcc actcgctgca tttgtccacc
     5821 agataactga tttggctttt tatgcatatg atttgtcaag ccgactcggt gcaatgcatc
     5881 ttctgcccgt tttcgttttt cgtctttaga aacaccactt aatgtcatcc caagttcaac
     5941 gttttcaata atgcctagat gaccaatcaa gttgtaactt tgaaaaatga aaccgataga
     6001 gttgtttcgg taggcatccc agtcactgtc cttaaagtct ttagtagact ttccgttgat
     6061 taccatatca ccggaatcat aattatctag tccaccaatc acattcagca tagtcgtttt
     6121 accagaacca ctaggaccta aaatcgctac gaattctttt tgtctgaaag cgacagacac
     6181 accatccaat gctttggtgg tggtttcgcc gactttataa tattttttta tatcttttaa
     6241 ttgaagcata atcacccttc tcccgtattg aaatattcgg tatactaata agtatatcag
     6301 aacaatgatc gtagtatacg aaataaatgt taactgaaga tgaactacaa gaaaaaatct
     6361 agaaattttt caaaaaacag agggagatca atgaatcgta ttttagtagt agaagatgat
     6421 ccgcaattac agttgcttta ccgttctgtg ttggaacgag cgggcttttc agtcatcacc
     6481 gcattgaatg gagaggatgc gctgaaaaaa ctagaagcga cacgtataga attaatcatt
     6541 acagatatca tgatgccgaa aatggatggc tatgacttgc tagactcatt acgtagcacc
     6601 agatttgaca cacctgttct aatgattacg gcaaaggccg attttgagga taagaaacgc
     6661 gggttcaaat taggagcaga tgattatatg acaaaaccca tcgatgtcaa tgagatgatc
     6721 ctacgtgtgg aagcattgct tcgacgttca aagatcaatt acagcagtag cttgataatc
     6781 ggacaaacaa aattagagct agatgcttat acagtgttac aagatcagca ttcgttgatt
     6841 ctgcctcaaa aagaatttct gcttctgtat aaattactgt cttatcccaa caagatcttc
     6901 actcgccaac agttgatgga tgatatttgg ggattagata cgaatacaga agaacgaacg
     6961 atcgatgtac atatcaaacg tttaaggacc cgttttgaaa agacggaaga ttttcaaatc
     7021 atcacagtac ggggactagg ctataaggcg gttatttaaa gatgaaaaaa aagctatttt
     7081 cacaattatg gatctatttt gctagtattg tcttcatttc gatcttagtg acactgctgt
     7141 gttttctcgg ttttattttc ttattgtctc gacattctat ctcgcctgct gaatcggctg
     7201 gaaaaccaac gttatacccg ttattcgtct ttgcggggtt cagtatggtc gtgggtacag
     7261 gaatatcgat ttttgttggg cggcgtatcc tgcatccaat cagtgcgcta gggacaaata
     7321 tgagtctggt agcaacggga gatttttcga ttcgaatgga tgaacagcag aaagtagcag
     7381 aagtacagca actgtataaa gatttcaacg ttatggtcca agaactcaat agtatcgaga
     7441 cactgaggaa tgactttgtg tccagtgtat cccatgaatt caaaacacca ctagcaacaa
     7501 tccaaggata tgttcaattg ttacaagccc caaatctctc tgacgaagaa cgccaaatat
     7561 ttttatatcg gattatcgaa agtatcacgc agctttcaca attaacagag aacatcttaa
     7621 aattgaacaa actggaaaac cagcgtatcc aactggaaaa gaaagagtat cgattagatg
     7681 aacaaatcag agaagtaatc gttttttttc agcctaaatg ggagaaagaa cagctagaat
     7741 tggatatcga acttgctgca gtaaattaca caggcaatga agagtttttg taccaagtgt
     7801 ggctgaatat catggataac gccataaagt acaatcaaat caatggacag attcatatca
     7861 aactatttga gacagccaca gagattgtac tggaagtgac cgattcaggt gtaggaatga
     7921 atgaagagac cagagatcga atgtttgaaa aattttatca aggagataca agtcgtcaaa
     7981 tttctgggaa tgggctagga ctttcactgg ttaaaaaaat tctagaactg catgatggac
     8041 gtattgatta tagcagtatc gaaggagtag gaacgacagc aatgatacgg ttaagtaaac
     8101 aataggaaaa agaataaaac ccctaaaaat tctattgaag gtttttaggg gtttttagtc
     8161 gtttttttgt gattagggaa atgttcttac ggttcttttg gcataaaaat cgctgctttc
     8221 ttattgaact acgagaaatg ttagaggtgt cattgaaaca aggacatgat gcaagaaaga
     8281 gagcgaaagt catgcgtcgc tctctttttt tgggtggata gatggacaga aaatgtaggg
     8341 cttagttttc ttcttgattt aaatactccc aaaatgtatc tgaagccaaa gaatgctcac
     8401 cgactttacg agtaaatgca agataactct ctgttgttgg agaaataggg agaatcacag
     8461 tattttcttt taatagattc ttgactgttt tttccataac aatagcaatc cccatgtcat
     8521 ttgcaatcat attgatgatc aagtcgattc ttgctccttt ataagagatt tttggttcaa
     8581 atccagcttc gcgacacaga tctagtacag gttgatacaa gttcgtagtg gggccaagga
     8641 gcaaaaagtt ctcctttttc agttcgctta gatccaattt ctttttacta gctaaaggat
     8701 gatttttagg tagaactgct acaaaatcgt ctgtttcagt aggcaaccac tcaatcgaac
     8761 ttggtggggc ctggaaactt ctagcaaagt ggatcgtgtc actatgttca ttggcgtcat
     8821 tgtaagttaa ttcgacactt tccacttctt tcaactggac tgtaaattca ggatggcgtt
     8881 tcaaaaactc ggtgattttg gaaaaacctt tataattgat cattgtcggt atcgttaaga
     8941 tatttaatga aagattgttg tctttcgtat aggtttcaag cacttcttgc atagcatggt
     9001 attgttctac gacatttttt acatatggga gcaataagct tccagcttcg gtcaattcga
     9061 tttttctatg ggcgcgttcg aataatttga cgtctaattc tgtttctaac gaaagaatct
     9121 gtttagaaat atttccttgg gtggtgaatt gtcgatcagc agttttggtg aaattcattg
     9181 tttcggctaa atcaagaaaa acttcgaatt tatggatatc catcatattc ctcctactta
     9241 ttccaataaa gaatacttta acacaaaaaa tgaattggtg taatcttctc tgtctgttac
     9301 aatctttctt gcagcgaaag caataattac agaaattttt ctgatttttt tcgcttcgta
     9361 gaaaaagaga ggagtttttt gatgccaact ggagttttga tcaatgtagg ttccgttttg
     9421 tttggcggac taattggcgg attagtagga aataaattaa gtgaacattt taaagcacag
     9481 ctgacgatgg tttttggtgt gtgttcgatg gggatgggaa tctattccat tgcgccgatg
     9541 aaaaatatgc cggctgttat ttttgcttta gtgataggaa cagcgattgg tttgattgtt
     9601 catttaggta atgggataaa taagggggct gcgctgatgc aagtacctat ttctaaaatc
     9661 ttccctagtg aaaagcttgg tatgacccac gatgaattta tcagcacatt agtgacggtc
     9721 attgttctat tttgtgcaag tggtacgggg atctatggaa gtttagattc tgggatgaca
     9781 ggagacagta cgattttgat ttctaaatct gtccttgatt ttttcacagc agctattttt
     9841 gcgtgtaatt tagggtatgt cgtttctgtg atagcaattc cccaattcat tattttttat
     9901 attttgttct tattggcaaa atttatttat ccattgacta caccagacat gatcttagat
     9961 tttaaagctt gcggcggatt cttgatggtc gctaccggtt ttagaatgat caacgtgaaa
    10021 atgttcccag tagctgacat gatacctgca atgatcgtta tcatgccact gagctggatg
    10081 tggacaaatt ggattatgcc actgttataa taaaaatcaa agagaagcca ttttttgctg
    10141 actaaaaagg atcaaatctt ttagtcagtt tttttgtatt tgacaaactg attttgaaac
    10201 tatattatat tttgtataat atagattgaa ataaaatatt acacttgaga atagaaggtg
    10261 acgtaatgca catccagatt ccaactgttc ttcttgatgg tactgtgtta gcagcattaa
    10321 gcaaggaaga tatgtatggg tatgccttaa caaaaatcgt acaagaaagt ctctctgtca
    10381 gtgaatctac actatatccg gttttgagaa ggttgaaaaa agaaggatat cttgagacct
    10441 atgatgaacc ttttgaaggc agaaaccgtc gatattactc gttgacacca atgggaagtc
    10501 gtcatttaga agaaattatt gatgaatgga ttgtttttcg agattcttta gaccggttat
    10561 tggaggagag cacaaatgaa tgaatatttg aatgaagttg tggatcatct tcaagaaatt
    10621 cctgaagacg atcgttttga tttgatccag tattatgagg aatatttttt agattatgga
    10681 aaaacattag atcagatcat tgatgaatat ggtacaccaa agcagtttgc cttaaaatta
    10741 aaaatcagtt acttttcaga agaagaacaa gcagaaaacg agttagtgaa aaatccaaaa
    10801 agacaaattc gcctgatttg gttaatcatt atcggtttat ttgcttctcc tgtcctcatt
    10861 ccactagcac tggggtttat attggttatt gggggactat tggttgcttt actagcgctc
    10921 atttttagta tctatgtttt atgtatcagt atcttaggtg ttggcctatt catgatcatc
    10981 tcaggaatca gtgtgtttgg acaatcgatt ctgagtggat tattctttat cggtgtcggt
    11041 ctgttcgcca caggaagcgc agtatttatt tcgccactgt tgctgaaagg aacaaaaggg
    11101 cttttttatg tcatgatgaa ctttgttaaa tggatgggac gacgttttat tacaaaaaga
    11161 gatctgcatt cagcgggcgt ttgaaggagg acacataaat tgagattgaa acattttttg
    11221 attaccggta ttggtctgat gcttactggt ggcctcctag caggtgcaac ttatacatta
    11281 ggcgctcaaa agagtctgac ttgggataat ggtcctcggt tgatcgatat ggttactgag
    11341 acaaaaaaaa tcaaggatgc aaacaaaatc gtcatccatg cagaaaatca aacagtttcg
    11401 gtcatacgcg gattggattt tgagataaaa acgacttatg ataagacaga aaagccagaa
    11461 ataaaggaga aaaatggcgt gttgactgtg aatgcagtca aaaaatcaac aggtgcaatc
    11521 attagtttag aagacacaag tgcacaaatc actattgttg tccctgcaga tcatgaatta
    11581 tctgaattga caatcgaagg gaataatagt gcaattgata tcaacgaagt atcaatcaaa
    11641 aaagtgaata ctaagacaca tgatggctgg gtatcgttca atgaatttac aggaggaaca
    11701 ataactgcga aagatgaatt aggctcactt cgactagatg aggccaatgt ggataacttg
    11761 atcgtcacag gtgaaaacag caacttccat ctagaagaca gtttgatcag caaagaagga
    11821 aaagtcatcc taaacggtgg taatttatct ttgcatgaga tggaaaacag tggctatact
    11881 atccaaacgt ctggtgaatc ttatattgaa aaagactacc agccaataac aaatactttg
    11941 ttagaacaag gacagaaaaa gctaaagatc actgctaaag acacatcgat caatattacg
    12001 acggatcaag aggacaatga aacagattga tcctgtactc aactggatat tttaagaaaa
    12061 aacagcaaag atccttccaa tctttgctgt ttttcatcgc ttaacgcaca taactatagt
    12121 aaaataagaa agaaaatatc taaaaagaaa acagggtgaa agagtatatg aaggtagaag
    12181 aaggtcaaag agaaaagtta aagacagaaa tggaaagatt acatacttat atcactcaac
    12241 taagccaaac cttctatgat ccagacaaag aaaaagtgat ggttaattac ccgaacaatt
    12301 cagaaggtcg ccaattagag caagtgtacc atgaagtttt caaacattta ttgacggtga
    12361 agaaagagct ggattattat tctttaccga ttatcgatac aggaatattg aagtatgatg
    12421 ggaagaagga acgatttatc tttaaatctg ttcgggaaga tttgcctctt tcagcaggga
    12481 tggatctaga ggtcttggta gaagactatt ttacagaaga aaagcactgg gttcgaacaa
    12541 aattggatta tttaccgcag gcagccggag ggacacaggc aagcggttgg tacatcactg
    12601 aagacaaaga gttagaacta gaaggtgtaa tggcgcgcat tcgaaaaaaa attaaaaaag
    12661 taacattaga tatttattga aggataaagg agcgcaggaa gtagcatgag agaaaaaaga
    12721 gcattttata tcaatggtta tgcagggatt gtcggattgg tcgtattagc tttagttgga
    12781 ttgtttttct tctatttagg catgtggcaa gcgaaagttt gggcactttt tttaagtatt
    12841 tttctttggt tgatcacgtt attgttgtta agttcggcaa ctgtagtcag tcctaatcaa
    12901 gcaaaagtaa ttttgttttt tggacaatat ttaggtacga ttcgagaaaa tggctttttc
    12961 cttacgatac ctttggcaca aaaaatgact gtttcgttga aagttcggaa tttcaatagt
    13021 tctgttttaa aagtcaatga tttggatgga aaccccatcg aaatctcagc agtagtagtc
    13081 tttaaagtcg ttgatacagc aaaggcatta ttcgatgtag cgtattatca agattttgta
    13141 gaaatccaaa gtgagacagc cattcggcat attgctagcc aatacccgta tgatacattc
    13201 aatgagaatg atttgacgct tcgaggaaac acgacggcag tgtctgacga gttgcaaaaa
    13261 gagctgcaag agcgtttagc agtagcaggt gtcgaagtaa tcgaaacacg cttgaaccat
    13321 ttagcctatg caacagaaat cgctagtgcc atgctccaac gtcaacaagc aaaagccatt
    13381 ttgtctgctc gacaaaccat tgttgaagga gctgtcacaa tcactcaaat ggccttagag
    13441 caaatcgaag atggacaaga aatcaatttc accgatgatc ggaaagtcca attgatcaat
    13501 aatttacttg tctcaatcat tacagacaaa ggaacgcaac ctgttatcaa tacaggcgac
    13561 atgaaagacc gtaagtgaca tactttttgt aagataggaa gaaggagttt aggacggatt
    13621 attgtcttga actctttttt tagttgtttt aaagaaaatg acaaaattga aagaagaatg
    13681 atagttgatt cgaaggcaga attgttcgct tttttctata atcagtatat aaagtaagag
    13741 agaaggggaa aaagtgaaaa aacttctgat cggtctactt gttttcatcg ttgtggtact
    13801 tgcaggggtg aaaatcgcag attacgtagt tatgggtgga gaagaatact atgtacaaat
    13861 tacgacagat ggtacaaaaa cagtttcaaa atcagactct ggacaagaaa cagtcaacta
    13921 tagatatatc ctgcctggct atacgaaaga gggtaaggaa aaagaactgg acttcaaggg
    13981 acaattgact cgaccgcttc gtaaaggagc atatttaaaa atcacctgga acaaaaataa
    14041 aggtgtcact tcgtatgaag aagtcgaaaa aaaagacgtt ccgaaaacag cactggaaaa
    14101 actaattaaa gggtagggaa agaaatgaac aaaatagtcg aagtacagaa tgtaacaaaa
    14161 gtatacggaa aaaacaatga gaaaaagacg caagcattaa gcggcatcag cttcgatgta
    14221 gaaaaaggcg aatttattgg gatcatgggc gcttctggtt ctggtaaatc gacacttttg
    14281 aatattttat ccacattaga taaaccaaca gatgggcata tccgaatcaa tcaaaaagat
    14341 gtgaccacat taaaaggaaa tcaactagca gattttcgtg cgaatgagat tggctttatt
    14401 ttccaagact tcaatttatt agaaagcttg acagcacaag aaaatatcgc tgttcctctt
    14461 tctttacaag gcgtcaaacc aaaagaaatc aagcaacgtg tccaaaaaat tgcagaacgt
    14521 ttatccatct ctcatatttt ggaaagctat ccttctgaga tttctggtgg acaaaaacaa
    14581 cgggtcgccg ccgcacgtgc attgatcaca caaccaacga ttctattagg ggatgagcct
    14641 acaggtgcgc tggattccaa aagtgcccgg gatcttttag atacgatgga cgaattgaat
    14701 acaaaagatc atgtatcgat tcttcttgtg acgcatgatc cgttttctgc aagttattgc
    14761 caacgtattt tattcatcaa ggatggcggt atccatcaag aagtgaaacg tggagatcaa
    14821 agccgtgatt ccttctatcg agaaatacta tctattcttg gaaacttgga acagtagggg
    14881 gagattgact atgctttgga aattatcact tactgggatc aagagccgtt taagagacta
    14941 cctggtgtta ttctcaggat tagttatggc atctgctatt ttttacatgt ttgaatcaat
    15001 ggcaagcaac gaagcttttt tgaaaagtaa tacgatcgtt gcggctacag ttatcatttt
    15061 ccgattcggt tctgttttac tagggatcat cacattcgtt tatattttat atgcaaattc
    15121 ctttttgatg acgatgcggc aaaaagacta tgcaatgttt atgatgctag gagcaaaagg
    15181 cagaaaaatc gcacagatga ttttttctga aacattctta gtagggatcg cagctacttt
    15241 agtgggttca gctgtcggtg taggattgac aagtatcgtc caccgattat tagtggatca
    15301 gttagatctc aatatttcgc attttacacc ttttagtatt cacgggttgt tgatcactgt
    15361 tttgtttttt gctgttttgt ttttattagc tgctgttgtc aatgcttttt cgattgtcaa
    15421 aaaaccgata ttagcattga ttcgtgcgga tcagacacca acaagaatca agcagcataa
    15481 attcttgttt ttccttgaag tagtactggg aattgctttt ctaggctgcg gttactatat
    15541 gatgaaacac gtggggacct atcgagtctt tggtatagga gtagccttag ttacgatcgt
    15601 attaggaact tatttcattt tccattctgt gatcattttc tttttatcat tactaaagaa
    15661 atcagaacag atttcattga aaaaattgaa caatttcaca ttgtcacagt taaactttcg
    15721 tattcgtgaa tatacacaaa tgctctcaat ggtcgctatg ctgtttgcct tggcactggg
    15781 cgctttgacg gtaggattag gttttaaaaa tgaaattgca gacttgacag aaaacaattc
    15841 tggatatgat ctagtgatga ataatcctcg ggcagaagat cagcaaaaaa tcaaagaact
    15901 ttcaccaaca ctttctgctt cttatactca aaaagaagat gcgggaacga tttactataa
    15961 tgcaagtgaa tttgcggctc aaccattgat catgataaaa catgaagatg gaacaccgcc
    16021 gaagatcacg aaagaaaaag ttcctgctga aaaaatgaat gaactgactg tacaagagga
    16081 acttcgaagc tatgaattgc cggaacagca agaaaaagaa atcaaggtag tcagtcaatc
    16141 agaatttgat caactgaact tgccacaatc cactttacag ctagttcagg taaaagactt
    16201 ccaagcaaac ctgaaaccaa tcgaaacact tgttgctcaa aataaaatga acaatccatc
    16261 tttgcaaaaa gtagagtaca gcaaccagaa atatgaaatg tatgaagtgt ataatggtct
    16321 attctctggt tttgaattta tgggattctt cctaggttta gctttcttga ccatgcttgc
    16381 tagctgtttg atgttcaaga tcttgtcagg aagcaagagt gacattgctc gttatgaaat
    16441 gcttgaaaag ataggaacac gacgcgggtt gcttaaacaa tcgatccgta gagaagtagg
    16501 cgtgttattc ttagcaccag gaatcttagg tgtagtccat gtcttattcg gattacaaat
    16561 gtttggtcta ttcatgcaaa atccatacaa agatatttgg gttccgttta cgatcttctt
    16621 cgtattgtat ttcatctact atgtactgac gacatggtta tatactggaa tcgtattgaa
    16681 aaagagattg tgaaaaagct gtcctgcatc aagtaataag aacagccaag caaaaatagc
    16741 tcctcctatt tttcgtttgg ttgggcttat taccgcagat gcaagctttt gaacaccgtt
    16801 tgttcggaaa aagaggttgt gacaatattt ttgtcacaac ctttttttca cggtctttta
    16861 ttagaatata gtaaattttt tctcgttact cacggaataa gctgttaaga aaacggtttc
    16921 catgtatact ggaatcagtg caaatgaaag aaaagagggt gcaaggaaca tggaaatcaa
    16981 tgaacaaaat tttggcgaaa actatcactt gattacgatc acgaatcaaa atgggacatc
    17041 tttgtctgtc agtgatcttg gggcacgggt tgttcatttt caaacaataa tcgataatga
    17101 aaaacgagag ttgatcttag gttttgatga agcaaaagaa tatctagaaa aagaccctta
    17161 tatcggtgct tcaatcggac gtatggctgg acggattacg aatggcacat tcaaattaga
    17221 tgatgaaacg ttcgaagtaa aaacagaccc ggacactggt cattgtttac atggtggtgc
    17281 acctagcttt gaagccaagc agtggcatta tacagtcatc aatggtgaaa acgaagcatc
    17341 ggtcattttc tatcagacta gcccagacat ggaaaacgga tttcctggaa ctttagatgt
    17401 agaagtacgg tatacgctga cgaacgatga tacatggcga gtgacggtcc aagggatcag
    17461 tgaccgcaaa acagtattca atcctacaaa tcatgtctat ttcaatttaa ccggtgatcc
    17521 agcacagtca atcgacgaac atatactttg gttgaatagc cgattatttg ctacgctcaa
    17581 taaagataca acgcctacag gagaagtagt ttcagtaagt ggcacagcat ttgacttcca
    17641 acgtcctaaa aaattaggtg aggtctttgc cagtgatttt cgccaatcaa tgatagtggg
    17701 cggtatcgat catccatttt tgttgaaaaa acaatctggt ttactaaatg ctgcggaatt
    17761 gacaagccca gatgaaaaag tatcgatcca agtaaaaaca gatgcttcag gaatcgtgat
    17821 ttttacagca aattttggag aagatggacc ggaaatgcgc gggaataaat tagtaaatca
    17881 tggcggtatc acatttgaga ctcaagaact gccaggagcg gagcgacttg aaagttttgg
    17941 agatgtcact ctggaaccta accaagtacg cgagtttgta acggaataca aaataaatat
    18001 aaagaaatag aataaattaa aaagggagtg tgacagtgtc acatcccctt tt
//
LOCUS       DAKBVG010000035        17589 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000035 DAKBVG010000000
VERSION     DAKBVG010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 17589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17589
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.035"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(177..1469)
                     /locus_tag="OUD09_000881"
     CDS             complement(177..1469)
                     /locus_tag="OUD09_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCT9692168.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(1466..2089)
                     /locus_tag="OUD09_000882"
     CDS             complement(1466..2089)
                     /locus_tag="OUD09_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9692169.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(2092..2928)
                     /locus_tag="OUD09_000883"
     CDS             complement(2092..2928)
                     /locus_tag="OUD09_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCT9692170.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(2945..3832)
                     /locus_tag="OUD09_000884"
     CDS             complement(2945..3832)
                     /locus_tag="OUD09_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCT9692171.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(3848..5131)
                     /locus_tag="OUD09_000885"
     CDS             complement(3848..5131)
                     /locus_tag="OUD09_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCT9692172.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(5814..6497)
                     /locus_tag="OUD09_000886"
     CDS             complement(5814..6497)
                     /locus_tag="OUD09_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCT9692173.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(6515..7132)
                     /locus_tag="OUD09_000887"
     CDS             complement(6515..7132)
                     /locus_tag="OUD09_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HCT9692174.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(7142..7966)
                     /locus_tag="OUD09_000888"
     CDS             complement(7142..7966)
                     /locus_tag="OUD09_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HCT9692175.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(7959..8519)
                     /locus_tag="OUD09_000889"
     CDS             complement(7959..8519)
                     /locus_tag="OUD09_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9692176.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            8693..9649
                     /locus_tag="OUD09_000890"
     CDS             8693..9649
                     /locus_tag="OUD09_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCT9692177.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMIHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(9954..10910)
                     /locus_tag="OUD09_000891"
     CDS             complement(9954..10910)
                     /locus_tag="OUD09_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HCT9692178.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCERLLTFIRESQEDAREHQENYWLRQPLYYETCFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILARIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            11015..11617
                     /locus_tag="OUD09_000892"
     CDS             11015..11617
                     /locus_tag="OUD09_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCT9692179.1"
                     /translation="MQFGRFRLGMRTIKSAIAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(11705..13204)
                     /locus_tag="OUD09_000893"
     CDS             complement(11705..13204)
                     /locus_tag="OUD09_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HCT9692180.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMNEE"
     gene            complement(13220..13744)
                     /locus_tag="OUD09_000894"
     CDS             complement(13220..13744)
                     /locus_tag="OUD09_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCT9692181.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            13919..15568
                     /locus_tag="OUD09_000895"
     CDS             13919..15568
                     /locus_tag="OUD09_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HCT9692182.1"
                     /translation="MFPFFKKKPLKLQNTIILVVCFSTFVSLIVSAILIRNFILSMNR
                     SSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIMIASNLDFIVVMDPEGIR
                     YSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFRYFVPIYYNQEIIGAVCV
                     GLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMKKVLLGLEPQEIAEQLSE
                     KTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDFEVGKKISDNLYDVLFKE
                     TIEHQTTVRTQSMYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGTKQ
                     YINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVPAI
                     AGFLLGKINEAKEQSVEMLLETDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQKHE
                     RNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLIHS
                     IVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            15537..16253
                     /locus_tag="OUD09_000896"
     CDS             15537..16253
                     /locus_tag="OUD09_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCT9692183.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     LKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(16355..16642)
                     /locus_tag="OUD09_000897"
                     /pseudo
     CDS             complement(16355..16642)
                     /locus_tag="OUD09_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
     gene            complement(16802..>17589)
                     /locus_tag="OUD09_000898"
     CDS             complement(16802..>17589)
                     /locus_tag="OUD09_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCT9692184.1"
                     /translation="ALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSSGKYYI
                     SLLCKEEVRELPKSNSAVGIDLGIIDFAILSTGQKFDNNKFTSKMEKKLKREQRKLSR
                     RALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEIIKNHDIICIED
                     LNTKGMLRNHKLAKSISDVSWSSFVSKLQYKADWYGRKIIKVDKWFPSSQICSNCGHK
                     DGKKSLEIREWTCPICHTHHDRDINASINILTEGLRLHSMGLA"
BASE COUNT         5227 a         3674 c         3100 g         5588 t
ORIGIN      
        1 gtttaaatag ttatttttat tttctcctat ctaaaaaagc agcccacata aaaagaaggc
       61 atgacaaaaa gcatgtcata gccccttttt ccgaataaat agtgttcaaa agacagccta
      121 tcggcaataa ggcaagattt tataaactac gggaagtaat ttccgtaaat ctattcttat
      181 tcttttaagc tgacaagctc gtcaatcact actgcttttc ctgaagcaat agaatgattt
      241 gctgcgattc ctgtcaaaat cgacaaaata ccatctacgt gagttgctgc ccgattccac
      301 ggatcttctt ctttttcttc accaaataga tcatctaata aacgcggatc tcctccgcca
      361 tgtccgcccg cttcttgttc gacgattacc tcatatggct caccgaacat cggatagaca
      421 tggatggact gaagaacggc tgctccttca tcctttttat ctccacctgc attgatgtaa
      481 gacttttcga tcactttcat ttcgatccgt cctttgctgc cattgaaaga aacattgaat
      541 ccttcccacg gcatatacgc gtttagcgaa tacgtcaaaa tcgcaccagt ttcatatttg
      601 accgttacgc ctagtgtatc ttcaatagag atatcatcgg aaaatacact gcggtcacga
      661 atatatccac tgtctttttc tgcttctaaa tacatttctc gcaattcctc attggctgac
      721 aggtctatag caaatggatc agctttagct agttcgcttc catagcatcg actgtaaaat
      781 atttctactc cgcgtttctc cgcattatgc atcccataaa agtttaatcc accgaatgca
      841 aatacctttt ggggacggct gttcaaccaa aaattcacta gatcaaaatg atgggtagat
      901 ttatgaacaa gaagcccgcc gctattgtgc ttatttcgat gccatcttct gaaataatct
      961 gccccgtgtt gcgtatttaa cagccattcg aagtggatag aaaacacatc accaatcaca
     1021 tcactttcta tcagttcctt tatcttcgtg tgatacggtg cataacgata attaaaagtg
     1081 actcgcaggc ttcttcccgt ctgtttttgg accgttatta tttcttgagc tttttcctca
     1141 tcgatagtca gaggtttttc gcaaataaca tcatatcctt ttttcattgc acgaatgata
     1201 taatcatgat gcgttcgatc tactgacgta ataatgactt cagccggccg tacgtcatcc
     1261 agtagttgct caaattgtga tggcatacat gtctttactt ttttctgtcc atacttgttc
     1321 acaagtatcc gatttgcata atccatcctt gtttgagata catcacaaaa accgactagt
     1381 tcacaagttt gatgatacgt agaggcaatt gcctcataaa aaaaacgcgc tctcccacca
     1441 agtccgactt gtacatattt tttcttcatt taaagtcatt ctccttttct tttaagcaaa
     1501 atactgtgac aaatggcagc cctggataca gtaatgcagt gagtaaaaaa tcatgctgta
     1561 tacaccagaa cagtccatac aaaaggcata aataccacag aaaccccttg acgttttttg
     1621 acaacagcca gatattcctt ttgatcaaat aaacaagact atctgcttta tcttctttgc
     1681 agatatcaat tagcgtacta aaaaaactat gggatacaaa aagcaacaga cacaatagaa
     1741 aaggaatcag ataccaagct ccaatctttc ccactaataa ataccaatca actgcaaaaa
     1801 taatgacaaa cagctgaaac aacgtaatga tgaaacacat ccgtttgatt tttcctttcc
     1861 tccaacatac ctttttttct gccaagtaat ggacagatag ataataagaa ggatacatca
     1921 tcaaaaaata aatcagatag acaacacgag cagacagcac taatcctaaa gtgaacaaac
     1981 atcctagaac taaagcgaaa agagcattac tgataagact ggttaaaaaa atccgacaga
     2041 catagtagtt gagcttgtaa agaaacatct cttctattgg ctttttcata ttcagccctt
     2101 cagaccgctt gtactgatcc cctggacaag atatttattg aagaataaga aaatcagaaa
     2161 aaccggtagc agcgacaacg tagacatcgc aaacatactc gcgtaagtcg tactagatcc
     2221 agcgtctgca aataatttga tggcaatcga taccgtgtaa gatctcggct tgtttaaata
     2281 aatcaatggt cccatgaagt cgtcccattt ccaataaaac tggatgatga tcgtcgtgat
     2341 gatcgaaggt ttcagcaaag gaaaaataat tttggtaaag acactatact tgctgcatcc
     2401 gtcaataatc gctgattcat ctaactcttt ggggatgctg accatgaatt gcatgatctg
     2461 gtaaatgaaa aatgcttgtc caaaaaaatg tggtaaaatc agcggcacat acgtcccaac
     2521 caaattcagc cgattgtaaa taataaactg cggaatcaaa atcacttgtg caggaatcat
     2581 catcgttact aacatgacga cgaaccagaa tctttttcct ttgaaatcga ttctcgataa
     2641 tgcgtaagca atacaagtcg agctcagaac tgtccctatc gtcgaaactg cagagatgac
     2701 aaacgaattc ctaaagaaag taaaaaatga aattccagca aaccccgccc atccttttgc
     2761 gtaattttcc cattgaaaag tctctggaat caaattcagc gaaccactca taatctcttg
     2821 atccgtttta aaagaacctg cgatcatcca taagaccgga taaatcatga ctagacctaa
     2881 tagcaaaata aaaccgtatt gaaaccattt cgttactgta ttattcattt cttcctcctc
     2941 tcattcacac aacgacccat ttttttgatg atttgaaaat cagaaaggtg acagctccca
     3001 taatcaacag catgatccac gaaatagcac tagcataccc catagaatta aaagaaaaag
     3061 cttggttata cacataaaga gcataaaagt tcgtggcatc attcggacca ccttgggtaa
     3121 tgataaatgc ctgcgtaaaa gtcataaacg ccgcgattgt ctgcataacc aagttataca
     3181 aaatgatcgg cgaaagaaga ggtaacgtga tcttgtaaaa cgtctgccat ttgttcgctc
     3241 catcgatctc tgccgcttca taatacgagc ctgggatctc tttgatacct gcagcaaaga
     3301 tgatcattgc cgaaccgaat tgccaagcag acatcaaaac aagagggatc atgaccagtt
     3361 tttgatcgct aaaccaggaa agcgcaggtg cgcctaatcg ttccaggaaa atattcacta
     3421 acccgttttt agaaaacaac tcccgccaaa ctagagcgat agaaatactg ccaccgatca
     3481 aactaggcac ataatacaaa gaacgaaaga aagtcgttcc tttgatattc ctcgttaaaa
     3541 gataagcaat caatagacca aagatcagtt tcacaggtac agatgcaaat acatagagaa
     3601 aagtgactcg gatggagttc aaaaaacggg catcactcag catctttata taattatcca
     3661 agccgatcca tgagactgga tttcccaaac gataatcggt gaacgaataa taaagtgaca
     3721 agatcatcgg tatcactgtt atcaatagaa atcctaggat gaatggagaa gcaaatacgt
     3781 aaccgaccac attgtcttga tccaagaacc gttttagttt ttcttttttc atgccttcct
     3841 cctgctgcta tcctagaatg tttgacgcaa actcataaag actctctgct gcttcttctg
     3901 ctgttacttc ttcataaatg actttttcaa ctaaaagatt gtattggtct tgaatttccg
     3961 gattcaaagc actatcaaag acattcactt cgccgttgtc catatcggaa atcatttcta
     4021 aataatcaaa tgtcgcttgc aaagttgcat catctgcacg agcgagctgt tctcgaatat
     4081 tcgacatgat tggtacacca cgttctcctt ttaaaatttc attggcttct tcactgtttt
     4141 gaaagaaatc aataaactta gcagcttctt ctttatgggc tgagcttttt gccatactca
     4201 gcatttgcga agaacgtaaa gggataccag attctccatc tgcttttctg cgaggtggat
     4261 taatcaactg aatctccata tcttcaggag cccctgacaa tatttctgtc aactggttac
     4321 ttgatagaaa caccattgcc gcttctctat ccctaaccgc ctgaaaagac agatccgtcg
     4381 tacttgcagc tccaccacct ggttctggat gggccccttt gtctaccaga tttttacgca
     4441 gctggaaaaa atctgttaga tagtctggat tttcaaatcc caagctgtta gatagatctt
     4501 cccgattata aaaattcaat cctttctcaa cttgtggtat ttgaataata cagccagcta
     4561 taaaatcttc gaatcttgaa atagcataga ttcctaattc ttcagtgatt tgatagctga
     4621 tttgttcaaa ttcatcccat gtccaatttg aagctggttc agggatattt aattgttcaa
     4681 atatattttt attgtaagca atcgcaaacg aattcacccc gttagacagt ccaactaatt
     4741 gcccgtcgta agtcgtcgtt gctaaatagg attcatctgc attagaaata tcaattatcc
     4801 cttgctccac atattcatct aatggttcaa tctgattgat gtattgagga aattgattgt
     4861 tcagttggaa aatgtcccag atatcatctg atgctgctag tgtacctaat tttgtgtaat
     4921 atccttcata atcataaaat tcataatcga tcgtcacatg gggatttttt tcttcataaa
     4981 gctcgattac ttttactgtt gcatcgtgtc ggacttgcga accccaccaa gccatgttca
     5041 gcgtgatttt tttgtcatct tcttgcccac aagccgtcaa gaagaataac ataacactta
     5101 aagctaagat gattctcatt cttttcttca ttctctgcac ctctacttcc attgttattt
     5161 ttcaatatac tgtattttta tacagaacat atttattaaa cgatcttttt caaggggaat
     5221 aaaaaccaag acttgcctta tcgttctgcc taggatgaac aactcaatac tttcaagaaa
     5281 aattagatag tgctgattct tctttcatcg ctaaaaacac ttcttgcttc aatgatggga
     5341 ggataattac gcaaagaaaa aaggatatca gacaactgcc caatatcctt gacatagcta
     5401 aataagcatg cttcttcgtg tgcgctcttc cctaaaaaac agaaaaataa ctagtaattc
     5461 tgtatgaaat taggtacaat atacacgtcg atgcacaagc attgactatg gcaacttgat
     5521 tggtcagttc ccgctgactt ttcaagcccc gacaatttag ctgctactaa attgtcgggt
     5581 gcctttttta gtttttattc gtttttcatt ctatagaaag cgcttcaata aatcaagaca
     5641 tttttttgtt agaatgctaa taataacaaa aggttttaac tcttcttatg tacattacag
     5701 gtaaaaaaac aaaaaaattt ctcttttttt tgaattctga acaaatagta ctggagtaca
     5761 aaagacgagg aagagccact atctgctctt cctcgtcttt tgattctcac ctgttattca
     5821 tgattctttt tctcagtttc ccgatatttt tccattttac gactaaaaag ctctccgcta
     5881 cttggtggcg tatatgtgat ggcatttgct cctgcttgaa tggtctcagc aatactttct
     5941 tccgttggac cacctgtcgc aatgatagga agttcaggat attttttacg aaaatggcgt
     6001 actgcttctg cagtttcttt acctgcactg atattgataa tcgaaatacc agctgctaac
     6061 ttttcatcta tttctgtata tttcgacgtt accgtactga taactggaat atcaactact
     6121 cgacaaactt cttttactgt ctctaaaggc gtaggaccgt tcaaaacgac ccctaacgaa
     6181 ccttgcgctt ctgcgaacat gctcatgtag gaagaacgag caccttgtgt caaaccacca
     6241 cctacaccgg agaaaactgg aatgtcagct gcctcaataa tgcttttagt aatcgctgga
     6301 tgaggtgtaa aaggatacac agcaattact gcattcgcat ttgtattacg tatgatcgca
     6361 atatccgtag taaagatgat cgaacggatc aattttccaa atatcttgat cccgcttgct
     6421 tctaaaatga tttccggtac tcttacgata tcttttctca attccgtcat gatttctggc
     6481 gtaaattgtt ctgccatcag ccacttctcc tttgctatct ccggtcatac tgattcaatt
     6541 gatacgtctc gatgacctgg atgatttttt gcgcataacc ggggtctgtc gcataacctg
     6601 catcttgcaa tgcttgtgcg gcttcctgat agttggtggc cgtaatcact ttttcataca
     6661 gcgcaggatt ccaatctact ccttggacga acagttgtgt atggtcatcc atggactgtt
     6721 cccatgaatc atacactttg aagtctcctt ggatagttat ccattcttcg ttaacaaatt
     6781 cctttgtttc caggcttact ttcttctgat ccccataagc cttgatcccg aaaagattat
     6841 tgtatttgct agccaaggta ctttttcccc aatttgattc taatatagct tgtcctaaaa
     6901 tgatactagg aagcactcca taaccatctt gtagttcttg ggcatgcgga acaagccgtt
     6961 caataaattg ctgatgggat tgcgcttcat tttcttgctg attatcagta aaaggagaag
     7021 acagattctt caaggaaaaa acaaaggcta agagtatcac tgctacaccg gcaaccactg
     7081 ctggccaacg gatattcttg cgcttttgct ttttccactt ttttttcgcc atgatcaatt
     7141 cctacttttc atttaattca ttcagatatt cttctagcgt caattggtgt ttggtgatat
     7201 actcagcgtt ttctttaccg acataacgta agtgccaagg ttcatatgtg atcttagtta
     7261 tatcttcgtg tccttttgga taacgaacga taaatccata ctgcgcagca ttatctgcga
     7321 tccatttagc tccaggctga tctccgtaac ttgcatccaa tacagtagaa ggataactgt
     7381 tgtaccagtt ttgatcgacg acatcgactg ccagacctgt gtggtgttcg ctgtatcctg
     7441 gctccgtaat cgtttcttta gtaatctttg tcgcctcctc ttctgataca ccttgacgac
     7501 tcatcacatc ttggacattt tgttcgaaaa cttgttgttg ggatgataca gaccggtaag
     7561 ctgagaccat cactaatgga aatccagctg cttcagcagc cttggcaaat gcttcatatt
     7621 ctggagcaat gcgtttgtcg atcaagtatc cgttacttaa tgatgccaat tgagtagact
     7681 catcaatttc tttttctaat ttgtgatctg gaccaaccaa aactagcttc caatcgtctg
     7741 ttttaacatc tggcaattgt ttggacgtac gatcttttgt ttctttgtcc tttaccttgc
     7801 tgctggttgt ttgactgttc ttttctttac tagcagttgt tttggtgctt gttcgttcga
     7861 ctgcttgatt gccggcttga acatgcgaat catcatcgct aaaaataaca tagcccattg
     7921 ctgcgatcat aatcacaact gctccaaatc ctaaaaattt attcacttga aaaatccttt
     7981 cgttgttctt cacgttcccc attcgcctca tgaacgtaag tttcttccag attttcattc
     8041 acccaatcga gaagttcgat tttttctcct tcgatctgtt tttgaacatc tgctggcaga
     8101 cgaaaattca aaatatcatc atagccccat tcatcatatt gaatacttac tttcaagtca
     8161 aggtatctac cagcctgctg attatttttc aaagggatca gctcgacttt tgctgctttg
     8221 ctgctaagcc atttttgcgc gatgcgtgtt ttgatttgct tgtactctgc aacggcaacg
     8281 tttcgttttt cgggaaaaat aatcgtttga cgcaaaatct tttgcgttag catccattca
     8341 taatcagtga aaagattttt tactttctgc atcgcttcag atggcagatt tttctcgcca
     8401 tctttccatt tttgccattg ttctggactg atcagcaagt attcatctaa aaaatttttt
     8461 tcagtttcaa actgttctaa taatacactc ataataagat caatatatac ttgatgcacg
     8521 tttatcccct ccttttaact attctactta agttttgatc aaaaagcacc tcttaaatcg
     8581 ctgctttcat tttttttaat gtttcaagtc ctctcctctt tattcttcgt gatttttgtt
     8641 ataataatag acagacaaga aactcaatcc aactatgaag gagcgttttc agatgaaaaa
     8701 atatcaatgg ggaatcgttg gtcttggtac catcgcacat gagtttgccg aaagcttcaa
     8761 tcaagagacg agcgaactaa cagcagttgc ttccagaact tcagaaaaag cggaaaactt
     8821 cgctcaccgt tacaatattc caaaagctta cggaagctat caagaaatgt tagacgatgc
     8881 tgaaatcgac atcgtgtaca ttgctgtacc aaatagacag catatcgacc atatcttggc
     8941 agcattggaa gcaggcaaac acgttctatg tgaaaaagcc atcacgatga ataaaaaaga
     9001 actggctgac gctatgcgct tagcagaaga aaagaatttg atcttagcag aagcgatgac
     9061 aatcttcaac atgccacttt atcagcagtt acgctcaata atggatacag gaaaactggg
     9121 ggctttaaaa atgatccaag cacctttcgg cagttacaaa gaaccagacc ctaaaaaccg
     9181 gttcttcaat ccagaattag ctggaggagc actgttggat atcggtacgt atgctgtatc
     9241 tttcgctcgt ttctttttga gctctcaacc tgaagtagtt gcctctacaa tggttccttt
     9301 tgaaacaggc gtggacgaac aatcagtcac tatcttgcgt aacaaagaaa atgaacttgc
     9361 tgctgtcagc ttgacttttc aggcaaaaat gccaaaagtc ggtatcgttg cctttgagaa
     9421 cggttatgtg acgattgctg attacccgcg tgctgatcgt gcggagatct tatttacaga
     9481 tggtacaaag gaattcatcg aaagcgggaa tacatctgaa gcaatgaatt acgaaataaa
     9541 gaatatgata cacatgattg aaggtgactt gcccaatcgc tctctcttct tgacaaaaga
     9601 tgtcatcgaa atcttggatc aaatgcaagt tctatggaaa aatatgtaat tttaaaaggt
     9661 tgtgaaaaag ctgttctgca tcaagtaata agaacagtca aacaaaaata gctcctccta
     9721 tttttcgttt ggctggactt attaccgcag atgcaagctt ttgaacaccg tttaaggaag
     9781 aggtcgtgag cctgccgttc agctccgagt cacaaggagc aatttcaaaa aacagcttct
     9841 catgtttgtt gaatattgtg acttgtgacc gaggagttga caggagaaca ccgtttaaag
     9901 aagaggttgt gaactcagct ttttgacctg aatattagct gaattcacaa cctctatttt
     9961 tggatttttt gttgggagaa ttctgccttg atttcaataa aacttttaaa agactgcaaa
    10021 aactggaaca attctgctcg atcctcgaat tcagggcgac tagtcggcag aggcatttgc
    10081 cgatacaata cataaacttc ttcaatacgt gctaaaattt gtctgccatc atttgactct
    10141 gaaaatgttt ctgcagtata aatcaataaa ccatagatat gttttccata ataagctggt
    10201 tgctggattc tctctagatt ttccagcatg tctttaatca cattggcctg tgctcttctc
    10261 atagagaagc aagtttcgta gtatagcggc tgtctgagcc agtagttttc ttgatgttct
    10321 ctggcatctt cttggctttc gcgaatgaat gtcagtaaac gttcgcattt gatctgcaca
    10381 tcttctttat tttctgataa aagaaattct gccatttgtc tcaaaatatt tcgaaattct
    10441 ttttcgatct gtatttggtt ttccttcagc tgcttcgtac gatctggcat gtaaacattt
    10501 gcaagcaaag caaatactaa tccaatcgcc attaatcccg cttcattgcc gatcagctgc
    10561 caagacatgt tttcctccac taaataatga gtgaccaaaa ccgaatttac tacgatccct
    10621 tcggttaatt tgaagcgaga ggcaagcggg atgaacagga ataaaaaaag actgaagcca
    10681 aaaatagtat acccaacagc aaaaaaactg aacagtgctg ttgccgtagc taatataaag
    10741 gctgcgattc gattcatgcc cgtacgcaat gtagagcgct tggtatttcc tacacttaat
    10801 aatgcaatga tccctgcagc tggccaatac aacagcgata agctacccgc tgtgaccatc
    10861 gaaaagaaga cactgatcac tgttttgact gttcgcattc ctatacgcat ttacttcccc
    10921 ctttttttat tttaattata cttgaataat gaaaaactag ctatcagaca aaagtttagg
    10981 tatgatagta aaaagaccaa aaaggagaat ctctatgcaa tttggccgtt ttcgactagg
    11041 tatgcgtacg atcaaaagtg ccatagctgt ttttttatgt atcttgtttt ttcatttgac
    11101 tcaacgagga atcccgatga ttgctgcatt atctgctgtt ttttctttgc gtcaggactt
    11161 gactacaacc gtttcttttg gaaagtctag gatcatcggc aatacattag gtggtacatt
    11221 agctatcatt tatttttttg tcaaaagcta ttttcacaat gattttttgg tagaattgat
    11281 cttgcttcct attctagtta ttattgtgat cgtcgtgtca gatggtatta acaataattc
    11341 cggtatcatt tccgcaatcg ctacgttgtt gctgattgcg ttgagcatcc ctcaaggcga
    11401 atccgctttg tatgctatcc aacgtgtact agatactttt atagggacat tcattgcaat
    11461 cggtatcaat ttctttcttc gtcctcctga aacagaaaag aaggaagaac tatcagaaga
    11521 tttggtcgaa ctccaaaaga aagaacgatt gctaagaaca aatttacaag agatagaagc
    11581 taagatccaa aaacaaaaga aaaatagcga ggactaattt tcttttgcaa taaaataaaa
    11641 aaatggagag aagaacttta aaaagtcttc ctccattttt gattgaacgg tgaagtgtac
    11701 tattttattc ttcattcatc gagttttgtt ctgcctcttt tggcgcttca tacatattta
    11761 cttggctagt agtcccacgt gtcgacaaat tctctacgat ttccttcaag tcaagtcctg
    11821 ttgtcgcttt tagtgtttct tgagtagaag aaagcaaatt tgtggcataa tttgtcactc
    11881 ggttcgctcc actggtttct cctgaactgc ttcctgtatc gacaacagag attttttcga
    11941 tatttcctag cggctgtgct gcttcgcgca tcaattgcgg tagcatatcg ataaccatac
    12001 tcaagacagc tgcttcgcca tattccttga aggcattggc gatcttttgt tttgcttcgg
    12061 cttcggcttt tccttttgct agtgctgctt cggcttctgc ttgtccagct aaacgtgtct
    12121 tattggcttc cgcttctgcc agtgcttcca cacggaatcg ttctgcttcg gcttcagtca
    12181 cttctttgac tttttgagct tgtgcttctt gttctttagc gtaccgatcg gcatctgctt
    12241 ttttcttcac ttccgaatca tattgctttt cacgacgtgt gatttctttt tcttccagtt
    12301 cgatttgttt ttgacgttct acgattttaa tctgcatttc ctgttcgatt acttgttgtt
    12361 gtgctcgggc actttctaaa ttgtaggctt ggtctgcttt tgctttagca acatcttgtt
    12421 cttgtttgta ggttgctaat ttcagttctt tttcttttaa tgattctgcg atctctgttt
    12481 gtcgctgtaa ttcagctgct tgtgattctt tttctgcttg agcttttttg atacgcgttt
    12541 cttttagagc ttccgcttct gctatatctg catctctttt gacttgggcg attcttggtt
    12601 tccctaaaga gtcaagataa ccgtttttgt ctctgacctc tttgatagtg aaagaaacga
    12661 tgatcaaccc catttttgca aggtctacag aagcgacttc ttgtacgctt tgactgaact
    12721 tatcgcgatt ttgatagatt tcttctacag tcatagaacc aagaatcgag cgcaagtgtc
    12781 cttctaatac ttctcttgct tcattttcca gttcttctct tgtcttgcct aaaaactgtt
    12841 ctgctgctgt agcaatctct tctacagatg aaccgatttt gatgatcgaa gtaccatcac
    12901 acatcaccgg tacgccttgt tccgtataca cttcaggggt ggagacgtcc aatttactcg
    12961 ataatagact gatccgattc gaacgttgaa aaactggcaa gacgaatgcg ccacctccac
    13021 gcacaatttt gattttgtta ttgctttcat caatgtgcac atttttgctt cctaaataac
    13081 ttccactgat gatcaacgct tcatccggtt ttgctgtctg gtacttcgct acaaagacga
    13141 tcagcaacat cagtaaaatc aaagctgcga ttgcgatcgc gataaaaaca actggtattt
    13201 ccatttaaca cacgctcctt taagctttta tcatgctttc ataaggcatg acataacaga
    13261 ttctttcctt gatctcaatg atcaatactt tcgttccccg ctcagctgtc tgttcttggg
    13321 ggctgtaaaa acaagctgga cgactaatgg aaccggtaac acttttcaac tgaatttctc
    13381 ccatacctcg tacagggatc ggtgtgatca caacagctac ttgtccttcc agagtttttt
    13441 cagaagacga catcgttgat tcggcatgtt tcatcggcat caatatatag aaattaagca
    13501 aaaataccaa cactatcgat atgcctccac tagcaataaa taaaaataaa gatgattgat
    13561 ctgttaaacg ctcacctaga aaaccaaaca agcttgtgaa agttaaccaa gggataataa
    13621 gcatcggatc aagcgggcca tcaaaatcga atacatctcc aaaaataatc aataggactg
    13681 ccaatcctgc acaaatcagc aatgtatacc aataaatcgt ctcaagtgcg attcccccta
    13741 tcatatacac gcctccttcc atttctacgt ctatcatacc aatgtttgaa aattctgtat
    13801 agcttctttt accgactcag tggtgttttt tataggaagg ttgtgtttat cgttgacaat
    13861 ttgttaaatt tttgttttca tgttaaaatt ttcacaataa attctttcga ggtagcctat
    13921 gtttcctttt tttaagaaaa aaccattaaa gctccagaac acgatcatcc ttgtcgtttg
    13981 tttctccaca ttcgtttctt tgattgtttc tgccatattg atcaggaatt ttattctttc
    14041 gatgaaccga tctagtgtgt cagataaaat ctctagcatt gctaaaatcg ttgccaatga
    14101 taaactcatc caaaaaaagc tgcagactgg tgacccaaca catgcgattc aaggaataac
    14161 aaatgaaatc atgattgcat cgaatttaga ttttatcgtg gtcatggacc ctgaaggcat
    14221 tcgatattct cacgcagacc caagttacat cggtcttcct ttctcaagtc atgaagatgt
    14281 gaaaaaagtg attggaggac aagaattttt ctctgaaaaa gaaggaatgt tagggttcgg
    14341 cttccgctat ttcgttccta tttattacaa tcaagagatt attggtgcag tttgtgttgg
    14401 cttaacgaac aacacgattg acaatcaagt caaagatgga caaaaaatga ttgttttttc
    14461 attgattatc ggacttagcg taggcatcat aggcgccgtt tttcttgccc aaaaaatgaa
    14521 aaaagtactg cttggacttg aaccacaaga aatagcagaa cagttaagtg aaaaaacgat
    14581 tatcgaaaat gaagtagatg aaggaatctt agccatctct tctgataaaa ctatcctctt
    14641 gatcaataca cctgcaaaaa acattttcta ccaaacagag cagctattgg atttcgaagt
    14701 aggaaaaaaa atcagtgata atctatatga cgttttgttt aaagagacga ttgagcatca
    14761 aacgaccgtt cggactcaat ccatgtattt aaatgggata gaaactattt ctagcgttac
    14821 acctcttttt agcaatgacc gttttttagg agcagtcgca actttacgaa atcgatcaga
    14881 agtaatgaaa ttaatggagg agcttggggg gacgaaacaa tatataaact ccatgagagc
    14941 tcaaacccat gaattcatga ataaacttca tgtcatttct ggtttgattg atttgaagaa
    15001 gtatgaagaa gtcacccatt ttatccagca gctaaatcaa aattataaaa aagagattgg
    15061 ccaagtctca agtctgatca aagttcctgc aattgccggt tttttattag gcaaaataaa
    15121 cgaagccaaa gaacaatctg ttgaaatgct gttagaaacc gattctttta tccctaaaat
    15181 ggagatggat gactccatcc attcactttt gcaagtgtta gggaacttac ttgataatgc
    15241 aaaagaagcc gtgctacaaa aacatgaaag aaacggacag gtccggattc atttaaacta
    15301 tgacacagaa gctgaagtat tcatcgtgac cgtctgcgat gacggtgtcg ggatatcaga
    15361 agaaatctca gaaaaaatat ttacgctagg tttttcgacg aagggaatta atcgtgggtt
    15421 tgggttgaat ctcatccatt caatcgtcaa acgccacggt ggatttttag aattttccaa
    15481 acaaacaaat ggcggtacaa tcgtctatct agaattacca aaaaaggaag atcaaaatga
    15541 tgacaaatgt cctgataatc gaagatgatc caatggttgc agaattgaat cgtacttttg
    15601 tggaaagcat ccctaatatg aacgtaatcg aaagtttcag aaatgtagaa gaaggaaaaa
    15661 aatatttcag acaaaaaaac gcagatctgc tgctgcttga tgtatacatg cctggcgaaa
    15721 gtggtatcca ttttttatct tggcttagac agcaaggaaa tgatatccca gtgattttga
    15781 tcactgctgc agatgatatc gaaaccataa agaatgtgat ccgctacggt gccttagatt
    15841 atttgatcaa accgttcact ttgcaacgtt ttcagctggc gatcgaaaag tatcttgctt
    15901 tctctcagac aatccaaacg actgaaaaag ccgatcagaa attgatcgat caagtattca
    15961 accacaccac tcccattcac gaaataccat cattcatcga tttgcctaaa ggtctgttaa
    16021 aactgacttt aaaaaaaata agtgcattta ttctaaaaga agatactatt ttttcaacaa
    16081 aagaactggc tcaaaaaagc tcactgtcac gtatatcagt caagaaatat ttgaattttt
    16141 tggtagaaca agatatattg aacgaaagaa tcaaatattt ggaaatcggc cggccgctaa
    16201 cctgttatga attgacaaag aatgggcgga aaaagctcac agattactgt tgagtcatcg
    16261 aattatccca tacaaaatag tccaagtata cagaagtaac aactgtttac ttggactatt
    16321 tttaaacatt ttattgacga atatccatgt tttcttatct taattgacgg gctttaggaa
    16381 gagatacctc atttttcgtt tttccagttt ctattatttc acgaaagttc tcgaaacttg
    16441 tcgaaatcat atttgataat gcctcatctg tatttgaacc tacatgcggg gttacaagaa
    16501 ctctagggta catttctact aattgctgga tagcagggtc tggaatcgtc tcccatggct
    16561 tgaaggatcg gaaaaaaacc tctgtttcat ttgcaaaaac atcggttgca aaaccagcta
    16621 aatgattgct tttcaaagct cgtgaactac tcaccactta gctaaaccta acggtttctt
    16681 acgcttgaag tgggagcttc ttgggaagag agcatacttg atagcgtatt actttactaa
    16741 cagaggtgcc tcttatttac caagctatcc ccgtagttcc tacggttctt gtttatttga
    16801 tctaagccaa tcccatagag tgtagcctta gaccttcggt caggatattg atactagcgt
    16861 tgatatctcg gtcatgatga gtatgacaaa tagggcaagt ccattctcga atttcgagag
    16921 atttcttgcc gtctttatgt ccacaattcg aacagatttg gctagatgga aaccacttat
    16981 ctactttgat gattttccga ccgtaccagt cagccttgta ttgtaattta gacacaaaac
    17041 tagaccaaga aacatccgaa atactttttg ctagtttatg attacgcaac atgccttttg
    17101 tgtttaagtc ttcaatacag atgatatcgt gatttttgat aatttctgta ctcaacttat
    17161 tcagaaaatc agtacgttga ttcattactt tttcatgcaa tcgtgctact ttacgttttt
    17221 gtttttgata atttttagct tcaaatagat tggtaccttt atttttagcc aataaagcac
    17281 gcctagacaa tttgcgttgt tcacgcttta gtttcttttc cattttggac gtaaatttat
    17341 tattatcaaa tttttgaccg gtagaaagaa ttgcaaagtc tataatacct aggtcaatac
    17401 caacagcaga attagattta ggtagttcgc gaacttcttc tttgcataac aaagaaatat
    17461 aatatttacc gctagaatga cgtgatattg tagcggattt gataatccct tttggttgtc
    17521 tatgaagctt aattcttact aatgacttca atttaggaac tttgatgaat ttactatcaa
    17581 tcaaagcaa
//
LOCUS       DAKBVG010000036        17533 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000036 DAKBVG010000000
VERSION     DAKBVG010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 17533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.036"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(42..254)
                     /locus_tag="OUD09_000899"
     CDS             complement(42..254)
                     /locus_tag="OUD09_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9692185.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            441..908
                     /locus_tag="OUD09_000900"
     CDS             441..908
                     /locus_tag="OUD09_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9692186.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSELNKYKLQKTSE"
     gene            905..1147
                     /locus_tag="OUD09_000901"
     CDS             905..1147
                     /locus_tag="OUD09_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HCT9692187.1"
                     /translation="MIASLFIYPEGGITVAKVKTPFEKAASKILERRGVSEIQWKKEV
                     LEKAQFDLLRGKDKDLEEYIYQREREKLVFDEIIKH"
     gene            1165..1467
                     /locus_tag="OUD09_000902"
     CDS             1165..1467
                     /locus_tag="OUD09_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9692188.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            1539..3593
                     /locus_tag="OUD09_000903"
     CDS             1539..3593
                     /locus_tag="OUD09_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9692189.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLIDLIR
                     LDVFKDTDETMSVEESYRNEKLLQVLEEELESDQRKVNQVSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETDEVEVDPVEA
                     NEESTWTEGDFQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKVKEELNKEEQRVIEEEVQKVKERYEKKRDNAYSLCVE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLEIRKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSESNE
                     VK"
     gene            3593..6205
                     /locus_tag="OUD09_000904"
     CDS             3593..6205
                     /locus_tag="OUD09_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HCT9692190.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFSEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSLENETAKKLLIQFYEEQAADRLQEYVEKLIKNKEISEMI
                     GKRLLLESEKVVKE"
     gene            6202..8733
                     /locus_tag="OUD09_000905"
     CDS             6202..8733
                     /locus_tag="OUD09_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9692191.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PEQAKGMAGNDKSGLSDIQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEDKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            8785..9117
                     /locus_tag="OUD09_000906"
     CDS             8785..9117
                     /locus_tag="OUD09_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9692192.1"
                     /translation="MDKYSNMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            9118..9711
                     /locus_tag="OUD09_000907"
     CDS             9118..9711
                     /locus_tag="OUD09_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9692193.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            9874..11568
                     /locus_tag="OUD09_000908"
     CDS             9874..11568
                     /locus_tag="OUD09_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCT9692194.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEIVKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
     gene            12256..14133
                     /gene="ltrA"
                     /locus_tag="OUD09_000909"
     CDS             12256..14133
                     /gene="ltrA"
                     /locus_tag="OUD09_000909"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCT9692195.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            14264..14641
                     /locus_tag="OUD09_000910"
                     /pseudo
     CDS             14264..14641
                     /locus_tag="OUD09_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            14641..15846
                     /locus_tag="OUD09_000911"
     CDS             14641..15846
                     /locus_tag="OUD09_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HCT9692196.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDP
                     LPFLNSSKK"
     gene            15862..16506
                     /locus_tag="OUD09_000912"
     CDS             15862..16506
                     /locus_tag="OUD09_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9692197.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQAANTYKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPTDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            16517..17323
                     /locus_tag="OUD09_000913"
     CDS             16517..17323
                     /locus_tag="OUD09_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9692198.1"
                     /translation="MSKKKGERQRFKKRKSYLRWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            17345..>17533
                     /locus_tag="OUD09_000914"
     CDS             17345..>17533
                     /locus_tag="OUD09_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9692199.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEAL"
BASE COUNT         6472 a         2734 c         3656 g         4671 t
ORIGIN      
        1 aaaaataaga aaagccttat ataattttgg cttttcttat tttatttgtt agttgaagta
       61 ttaatatcaa cgatttcttc agccaattta tttaatttca tagcatttat gaatctcatg
      121 ttgtctatac tagagcgttt gttggcaagg tcacttactg tagttaaggg tacgccagat
      181 tctatggata tagaacgaac tgatttacca ctatcaaaca gcaaccattc gattttcttt
      241 ttatcagcgt acatttcaat gctccttctt gttttttctt tattatagct cttttttgta
      301 atgaatcaca atatcaagag aaaaattttc tatagaaagg tggattttta tgagtattcg
      361 ttctccgtgt atccaaatga ggtttgttgc tactttcgtt atttgatata ctaaaaataa
      421 acaacgaaag aaggaataaa atgaagaaaa agcaactatg gtttgtctct atgtttgcaa
      481 tattttttat tattttaagt ggatgttcaa gtaataagaa atcggaagct aaagaagata
      541 atagtttatt gcaaccagga actgtttgga aagaagaagt aggtggtctt attcataatt
      601 taaaaattat agatgatact acctgggaat attctgaaag tgtatggcat ccagatcctg
      661 tacaaataac agttaagagg caaaaagact atgaaggttt agaaagatac aaaatagtag
      721 attctgcagg cattagggaa tttataaaca aacctgattc attatttatt gttgttccct
      781 atgagaagga tggcgtaaaa aaaataattt ttcttggatc tagcaaggat gagaagcgaa
      841 cgaaagagaa attaattcat gatggatcag agttaaataa atacaaatta caaaagacta
      901 gtgagtgatt gctagtcttt ttatttatcc tgaaggagga atcacagtgg caaaagttaa
      961 aacaccattc gaaaaagccg cgtctaaaat cttagaacgc cgtggtgttt ctgaaataca
     1021 gtggaaaaaa gaagttcttg aaaaagcaca atttgaccta ttacgaggaa aagataagga
     1081 cttagaagaa tatatctatc aacgtgaacg tgaaaaactt gtattcgatg aaattattaa
     1141 acattaaaag gagcgaataa tcttatgttt gaatcaatta caatgaattt tttaactcaa
     1201 ccgaaagtag tagctgcagt tgatattttt agagcattac aaactgccgc aactagtttg
     1261 aaaaataatc tgatgatttt tgctgctgtt gcggcagtgg cttccattgc tcttggaggt
     1321 attttcttct tctttgggcg aagaggagct gaaaacggga agacacacgt aggttatgta
     1381 ttgttggggg taggagttat tttagctgcc gcttctattg tagtttcaat gttctcaatg
     1441 tttggacaaa ccccacaagg actataaaaa aaacaagtag gaggagtaaa aagtacgtgg
     1501 ctacttttta ctcctttttt tatgaaagga gaatattcat gttttttacg atttcaggaa
     1561 atgcaaaatt ggataaagag ttaataagaa aagcaacgaa gaaagtggga aagcgtactt
     1621 cctttttacc gaaaacaatt aaatattctg tcattgaaac ggaagaacag ctacatgtgt
     1681 taggacaagt gttagaacaa gagaatcaat cttctgttaa attgactctc acgttacatg
     1741 ataaagaagg aaaaatagaa aaggctggca cagaatacgt ttttggccca ctggattatc
     1801 aaatttcaga aggtttttat cctacattga tagatttaat ccgccttgat gtgtttaaag
     1861 atacggatga aactatgagt gtggaagagt cttatcgaaa cgaaaaactt ttacaagttt
     1921 tagaagaaga gttagagagc gaccaaagaa aagtgaatca agtgagtgaa gtcgaagtaa
     1981 atgaagaagt accatgggaa cgaccaatct ttccaaagaa ggagtcaaaa gaaaaagaga
     2041 cagtctatgt tgacgaacaa gctgatattc cgactcagaa atcgatattt gaggaagaga
     2101 acgatgtgat gggtcaattg gaggtagatc cctttgaaga aacaatggag gatgaagaga
     2161 cggacgaagt agaagttgat ccagttgaag ctaatgaaga gtcaacatgg actgaagggg
     2221 attttcagga atatattgaa ccacttatca gtgaagaagt agaaaaaaac gaaacagaga
     2281 tacaaaaggc aactgtattt gattatattt tggatcaata tccgccagag catgaatgga
     2341 tgaaagaaaa aaacaaatta tttttaaatg agctttattc gaaatatcaa ttaccccaaa
     2401 cctatcaagc atttttagag agtaaagaag aattaatctc acagggtaaa ggcacacttt
     2461 tagaacagtt agagtctgtc caatcaacaa attggcatga gcttgcacaa gaagatttat
     2521 tagccacctt tgaagaacgt caacaagaaa cagatcaaga aattaatgag tatgcgacgg
     2581 aacagtcgtc taattgggag aaagtaaaag aggaactaaa taaagaagaa cagcgagtga
     2641 ttgaagaaga agtacaaaaa gtcaaagagc ggtacgaaaa gaaacgcgac aatgcttatt
     2701 ccttgtgtgt agaaaagatc aacaatttta ctaagacgaa tcaagaagcg ttatcgaaag
     2761 aaaaagaaaa cttgttaaaa gaacgtatta aagaacgaaa agaagagttc taccgcggtt
     2821 tacgtacaga taagctaaag attagcaaag aattaaatga acaactgaat aagctatatc
     2881 aaacaacgac tgaagttctt atggaaaaac acgaacagat ccagcaagaa ttacaagtac
     2941 aaattccaaa atggaaaaaa gagtatgaaa ttcaacaaca agaattgaaa gaacaagaag
     3001 aaaaacaacg gcaaaaagaa aaagagcgtg aacaattaga aataagaaaa cgggaacagg
     3061 atcttgcgga acaaaaaatg gcgatagaaa aacaacggtt agagcaagaa aaggaaaaag
     3121 aagcagctgc tcgtaaggaa cgtcaagaac aaatggatac attgcgtcaa gcacagttga
     3181 attatctagc acaacctcaa ccagtagctg ctgttcaaac gccagtgaac aaccaagcaa
     3241 atttaactcc ttcaccccag cgttccttta acggatggat ggtgggatgt attgcaagtt
     3301 taagcttgct tctaggtgga ggaacagtat ttgcttttaa tcatatcaat caatcttcac
     3361 aggctgattc tgtagccaca acggcgtcaa tcaaggaaga agcccaggaa caagcaagaa
     3421 aagagtatga acaacaatta gaagaattat tgaatcgtac caatcagtca acaacagagt
     3481 caagcatgga aaatagcaaa gagagtgatt ctagtaaggc taaagaagga tcaagcgatt
     3541 caactgtaca atcaaacact tccgaaagtt cagaatcaaa tgaggtgaaa taatgcgtgt
     3601 tagagaaaca acgaagcgaa aagacaaacg attcaattta gaaaagaata ggtatctttc
     3661 tcatttctat cgctatactc cctcaggaga agagctacca gaaaaacatg acaaacgcat
     3721 ttcgaaaaag attttttata gtggacttgt atacctccct ttagagtatt tagtaacaag
     3781 agtaccagaa gttttcaacc gattggaaat tgttaaggga agcttacaag gaataaatct
     3841 gtttcaatta caggaaatgc cacaggtctt tcattttgat tcagctggtt tttggaatcc
     3901 atttactcct acaactagtt tgggtacacg tttagcaatt tcgacagttt gtgtggttcc
     3961 agctgtactt atgggattga aaatggacat gtggtttcgt cctctagcag atggacaaaa
     4021 aggggataat cgattaatga ccttaaaaga aatccaacag atttatccgc aaatacctga
     4081 aacaaaaact acttttccag gttatggagg tattccaatc acacattaca aaaaatattg
     4141 gtacattcaa acagatactg ttaatacgtg tattgttggt acctctcgat cagggaaagg
     4201 tcagattgaa gtgttagcca ccattgacaa tttatctcgt gcagaaaaac aaagttcaat
     4261 ggtcgttaat gatccaaaaa cagaattata tgttggtagt aaagatgaac tagaagctag
     4321 agggtatgat gtatatgcct ttaatatcac cgatccactg caatcaatga gtgataatcc
     4381 attggcactc atcgtgaaat actggaaacg aggagacata gatactgcaa cacagttaac
     4441 caatactttc acaaatacga tttattatga tgccaacgct tcagataaca aatactttaa
     4501 cgaaaacgcg cagaaagctg tcaatgcgct tatttttgcg ttgttagaac aagctgatca
     4561 aacaggagat tactccaaat tgacaccgaa taatctcgtt gagcttcttt ctgagattgg
     4621 cggttttaat tattatccag atccagacaa tgaattcaaa cagttgaatg ccttagatga
     4681 atttatgaat cagttgccac ctggaaacat tgcaaaaaaa caatatgggg caacgaaaat
     4741 tggatctgat aaagcgaagg gaaatatttt atcaacggca atcacgggat tgaatccatt
     4801 tactctaacg aaaattgcaa agatgacttc tcaatcaacg attgattata aatcggttgg
     4861 gtttccaaaa tacttagatt tgaaattaca tgaaagtctt ttaaatcaac gagtcgtagt
     4921 agaattttat cataagggaa agaaatttca tgaagaacgt gtcaaagtag gctataaagg
     4981 atttagtgaa atcaattttg attgtcattt agttgcaggt gatcaagttt gtttaaaata
     5041 cgatcatcaa ggaaaagaat atgtggcttg gtatgaattt tctcaaagag tattgaaaga
     5101 cgaagagggt aatatcgttt atcggaaaaa acgaggagaa acccatttgc cagaattaga
     5161 aaaagaagcc gttctaacat tagatgagga aaaaagtaac ttattagtag agtctattcg
     5221 gatgcactat accgataaac caatggctat ttttatgctt acacctgatt atgatccatc
     5281 taaccatatc attgttagta tttttcttag tcagttatat aaagaattat ctacgcaatg
     5341 tgttaagact aaaggagata aatgtcaccg caggattcat tttattttag atgaatttgg
     5401 gaatatgcca ccaatggata atatggaagg aatcatgacg gtcacagctg gccgaaacat
     5461 gttgtgggat ctgtttattc aatcttatca gcaactttat gcgaagtatg gagaacaaaa
     5521 cgcagcgatt atcaaagaaa attgtcagac acatatttat attatgtcga cagatgataa
     5581 taccatcgaa gaattttcta aaaaagtggg gaataagacg gttgaacaag agaacagtac
     5641 catcaattca ttaggtatga atactcatat caatcgcaat gttgattcag atcgtatctt
     5701 aactcctgaa agaatttcta cttttttaga aggagaaacg attgtactcg ctcctttaaa
     5761 acgacaagat ttgaaaggtc ataaaatacg gccgtatcct atttttaatc atggggaaac
     5821 gaatcttcct tatgcttatc aatatattga agatttcaat ccatcgtggg atatcaatga
     5881 tatagacata cattgtacac atgcggactt ggatcttaag gctttacaaa ttgattatga
     5941 ttatttccta ccagatgaat atgctagaaa tctctatcat ggactgcatg atcaattaga
     6001 agaaaaaaac gaagaaaata tgttttataa aatcgttcaa tctcttgaga atgagacggc
     6061 taaaaaattg ttaattcaat tttacgaaga acaagcagca gatcgacttc aagaatatgt
     6121 tgaaaagttg atcaaaaata aagaaatttc tgaaatgatt gggaaacggt tgttattgga
     6181 atctgaaaag gtggtgaaag aatgatcatt ggaggaattt tttctggcat agccgactgg
     6241 tggtcaggct tattcggaga aggggatgca aaccccaaaa ccgtaacgaa agtcttacaa
     6301 gattatggga attatctgca gtatcaagat gtatttagtt ggatttggaa cacttttctg
     6361 tggggattga ttaaaggatt atataatctc aatactttac tagaaaaaac catttatcaa
     6421 agctttgatt taaaagactt attgaacgcc gctggggtaa atgcactatt tcaaagtttg
     6481 attagtaagg tattggctct tttttgtgtc gtcacattaa tttatttagg cttgaaattt
     6541 tttatgagta aacacccacc aaaagtcaaa aatatcttgg ttaatttatt tatggccatg
     6601 atcttgattt ttggtggtag ttctttaatc gatgaagggt taaatatcag taaaagcttt
     6661 tacggagacg tcactgcagc aaataaagca gataaaacgg gatcacctgc ttttcaaatt
     6721 attcaaaata atgtgatcga tgtcagtacg ttactagcaa ctgacccgaa taaagtagaa
     6781 tccctaccaa cagataaaag aaatgcttta acagcgaaaa attttaaaac agcgaatatc
     6841 aatgggatta ttactcctga acaagctaaa ggtatggcag gtaatgataa aagtgggtta
     6901 tcagatatac aaaaggatcg gttaaaggca ttacaatacc gattggaact agatgataat
     6961 gggaaagaaa ttcctgttca aattagtgac gaaggcttag caaaatatgt ctatacttca
     7021 gggtatcgtc gatacagtag taaaccaggt attattttag ctggtgaatc ttctttggca
     7081 gtagcctatt tatttatcct atttacgatc gttacttgca ttattgagct ggtatttaaa
     7141 aaattctatt tagtgattgc cgcgtcaacg gactttgaga caggtcaacg gatgaaaagt
     7201 gcgatagaag atatttctca atccttcttg ttattggcgt ttacgatttt agaattacga
     7261 atctatacct taatgctttc aggtatcggt gatttgcatg cggcaggaaa gatcaatgga
     7321 ttcttgtatg tcgttgcttt gattgtacta accattgcgc tattcaaagg ctctcaatca
     7381 gttacgaaaa ttttcggcgt tgatacaagc ctgaaaaacg gcagtaattc tcttcttagc
     7441 atgtttgcct taggaaatat tgcgaaaaat gtgggtagcg gagctaaaaa ccttgcaagt
     7501 gctggtaaga gtggtttaag ccatttaaat gatattcgta aaaatggatt tggccatcga
     7561 aaagatgtta cccctgataa cggggaagaa caatcagacg ataaagctac aactgtacct
     7621 aatagtaaga aaacagtttc cgaaatgttc gggaaagcga aaaatggtgt ttctaaagcc
     7681 gcagaaggtc ttggttacgt caatgaacgt gggttaaaag gaacagctga agatcttaca
     7741 gaaaaagcca aagacacctt agaagataaa ttagacggca cgagagctaa agaattggct
     7801 gaagctgtga agaatccatc cgaagcacta gaaaaacaaa aagaaaaagc ttcagaagtc
     7861 aaagaaggac taaaagatgc gtatggtgaa ggacaagtca atgcagcagt gaaagctaat
     7921 acaacaaccg aaaaagaaaa tcaagaaggt atagtgattc cttcagtcaa agatcaccaa
     7981 gaagcgttgc actctaatga aaattcgatt gaagagaaaa attccataga agaaagaaat
     8041 tcagaagaag gaaaaaatgg acagttacca aaaactgatc cagaacaata tcgctataaa
     8101 cctaacagtg accttgaaaa tgagaagcga atttcagatt tatcaaaagt aaatcaagaa
     8161 gacaaagcag aaaatgaggg acagttacca aaaactgatc cagaacaata tcggtataga
     8221 tctgttaact ccgaaaatga aaaacgcatt tcagatttac caaaacgaaa tcaagaagta
     8281 caaacggatg aggattccat cgttctgcca aatgataatg gaatcgaagt tggaactgaa
     8341 aaatcaacga aaattggttt acctaaggca caaaatttac caatgaaaga tagtcgtttg
     8401 aatcaattca aggataatga tcaaagtttt gttcatcaat cacctgcagg tactgttcaa
     8461 atgaaaccaa gtcgtttgcc tgaaaatgga aatctgacaa ctgtttctta caatttatct
     8521 gaaagtggtg agacagggaa tgcgtccgtt caaaatgttg tgacaagaga agctgcacca
     8581 aaacctattc aaaacgttca tacaggttac aagccagcaa atgtggcatt tactgcacca
     8641 acaaaaataa atgtgacaag agaaacaaaa aacaggaaca atgcattgaa agaacaacta
     8701 ttgagggata tcaaacagga tatcaaacca taaaaggcct acaagttagg ccttttttct
     8761 atgtatgaaa aggagaaatc atttatggat aaatatagta acatgaaaaa ccctccaaga
     8821 tttagtggag aatggaagtt tttcgctttt ttctttttag atctattaac gcttggtggc
     8881 tgcttttttt gggcgaaaat ggtcaataat cttgtccact taccactctt ttttgcactc
     8941 ttgaattatg gcttatggat tgcgatagcg ttagtgattg tttggcgacc ctataataac
     9001 ccaggtcaac gacaaatttt tgcgattatc gagtctattt tttttgcgga caagaaccat
     9061 tatcatgcgt tagatcctaa ccgaaattat catggtacgt ataaggagga tgaataaatg
     9121 cgagtcaaag gacaaaattc agttgaaagg aaatccattt tgcagtttgg aaaaccagaa
     9181 gagcgctcta tcttggactt atttccatat aaatcctgta cagaagaggg agtcttaatt
     9241 actagagaag atcattttca acgttattat cgtgtagttt ctacagacgt ggaaggatta
     9301 aatgaacaag agaaactaga acgaatgaat cagttgacca ccgtcatgcg tacgtatgtg
     9361 ccaacaatta agttagttag tttgaccaca gagacggatc taacggaaca aatcgtgcaa
     9421 aagcggcaat tattagataa aaatcgtatg gaacagtcct taggaagaaa tctacaacga
     9481 ttaaaaaaat atgaaaagaa attagtcgaa gaaatccagg aattgaaacg agcagaacaa
     9541 gaacgtcctg acttaagctt ctttttcgtc attgaaggaa agacaatcaa ggatttacaa
     9601 accagaaacc gtcagttaat gcgatctagt ggcattttag ggttaaaacc actctacaaa
     9661 aaagaattga tcaagattct gtatcgaatg aacaatatga atgatgagta ggaatggaga
     9721 agagagaatg aaatatccgt tgatatggtt agaaaaagat gttccgaagg aagagaccac
     9781 tagaaaattt cctcacactt taagcctggg taagttggga aaagggcaat cttttaacca
     9841 agtaaagaat caaacaagaa gaggtgactg tacatgttag aagcaattaa aacagctttt
     9901 tcaccacaaa atgatcaaaa agtacttgat ccattaaagc aactaggaaa tcgggaaata
     9961 gaaattgtaa aatccaaagg gtatgattta gattttttaa aacaagtgat gccacaaggt
    10021 gggttaaatt ttgatgaaga atatgtgacg tatggtaatg gttattcacg atgtgtgaca
    10081 gtttatcact acacaaaaaa tcctacgctt ttttggttgg ccaatctcat gaacaatcct
    10141 catacagtgg ccacactcga tgttagcaca gatgaaaagc aaaaagtgat tgatcaaatc
    10201 aatcgtgcct tagatgaatt aaatgatcga tcagataatg aacgaaaagc gactgaccga
    10261 aataaaagcg cggaagaata cctggaacta gcggaatatg ctagaaaatt gacttctaat
    10321 ggggaaattt ctaagcaagt caaagtgaga gtacatgttt acggaaacac gattcaagaa
    10381 gtagatgaac gagcgcagga actgatcgaa aatctcaaag ggatggatta tcgtgcggtg
    10441 acttatctgc ataaaaccaa acgagattgg caatcgatgt ttaccatgtt cgatgaacaa
    10501 gctgaatggt tcggaaatat caaagggcaa tcactaccat cgttaaacat tggcggaggt
    10561 ttcccgttcc accaccaaac gctaaaagat ccaggaggga cacaatatgg ggaaaccatg
    10621 acaggcggcc catttgtctg gaatgcgacg tataaaaata gtgtacggtt gtctactaat
    10681 accttgcttc taggaatgat gggagctggt aaatcgaccg ttcttaaaat gatcgcggaa
    10741 gcccatttag ctgcaggaga ctttatttgg ggctttgaaa agggaaaaga ctttattcct
    10801 ttcttaaagg aatataacgg tattatggtg cgtttagacg gatctgacgg aatgatcaat
    10861 cctttagaga tatttgccac acggacgtat gatgaagcat ccagtttata tgacgatgga
    10921 tcagtcaaag atttgaagat caatgaagca gcgtcctatc aaacacactt agacaaagtg
    10981 gtctatcagg tacagttagt gagtcctcag ctaaaaggaa cgatgaaagc agaatttaag
    11041 acctacttaa atcgttttta tgaagagtat ggaacagtac cacgcggctt tacttcctca
    11101 aattcgcgtt caaatactga tacgcaagtc acagggaaag atccagaagc ttatccgacg
    11161 ttcaaagaat ttttagactt tttaggacaa ctggaattac caggtgcttc ccaagagaaa
    11221 aagaatcgaa aagaagaaat ggaatccatc gtagaatcct tatgtgagac gtatggcatg
    11281 atttttgatg gtcatagtac gattcgacac cttgaccaac aacagttggt cttttttgac
    11341 attgactcta ttacaggctt agatcgaggg gtacaacaat gtcaaatgta catggctatg
    11401 acgttgattt ggaatcaggc attgattcaa ggaagaagac aacgacgttt aattaaagaa
    11461 aaacaaatgg aagaacaaga cttcaaacgc ttcttggcga tgattgatga gtgccataat
    11521 ggtgcgacct gttctgcagt gaaaagctgt gggttgcaag tagtctaaaa actattgtaa
    11581 taactcttat aacgagaagt caaatgagtg ggtaacgcca caaagggctt ccactgatac
    11641 tccgacatgc aacaagtagt ttatgcagac caacgttgtg tgaagctcgg tgaagtcggc
    11701 tgaaggatac cgtaaggtaa aagaaatagt gaataccacg aaagttctct agaggtaaag
    11761 ggtgtatccg ataagtcggg tgtccaaaaa aacttaatat ggtgagtatg tacaaacgta
    11821 ctgacgaact agcgaatgta ctgtccgaaa ggcatagctc ctagaaatgg gagagacagt
    11881 gagcaaaaaa tcactcattg tgctaacttg tggttgagta aaagtcgttg ttatgaaaga
    11941 ccatgctgcg ttacaggcgt aggagaagtt agggggcaaa agctagcatc taagttaagg
    12001 catgtggata gatataaggg aacaggggaa gccatcaaca tggagagtgc atactcgatg
    12061 aagtggtagt aagaaatatg tgaatgacaa ataacttact ttagtgatgg tgaaagtaga
    12121 gtcacgactg atgaaggttc tgtaatggaa ctggaggaac aggctcgatt cgtctgtaaa
    12181 agtcatgaaa cccgttcaac catgggattc tcgtatgact aaaaaggtca cgagccaatt
    12241 ggagcgtgat aaaccatgac aaaagaaaca agtccaaaag ataggaagtt gcggcatgca
    12301 gaatattatg gcatgacgga aatcttcgat aagatatact ccaaaagttt gaagggagat
    12361 aatttcaaga atctaatgca gattatcatt tctgataaca acattgtcct agcatatcgt
    12421 aatatcaaga gaaatcacgg tagtgtgaca agaggagaag atggagtaac gattaaagat
    12481 attgaaaatc tcaaccgtaa tgaattcgta gaaattgtta ggaagcgatt cgcacattat
    12541 aaccctcgga aagtaaggcg tgtagaaatt cctaaatata atggtaaatt aagaccgttg
    12601 ggtattccat caatatggga tagaatcgcg cagcaatgca tcttacaagt attagaacca
    12661 atatgtgaag caaaattcaa taatcacagc tatggtttcc gcccaaatag atcagcagaa
    12721 cacgcaatag cagattgtat ggcccgaatg aatcaaagtc acatggaata tgtggtagat
    12781 gtagatatca aaggtttctt cgacgaagtc aatcatacta aactcatgag acaaatctgg
    12841 tctatgggta tacgagacaa acagcttctc ttaatcatta ggaaaatgtt aagagcaccc
    12901 gtagtacttc caaatgggaa gttgatatac ccaacaaaag gaacaccaca aggcggaata
    12961 cttagcccat tacttgcgaa catcaatctc aacgaatttg actggtggat agcaaatcag
    13021 tgggaggaaa aagatgccaa agagctaaaa catcatttta ggaaaaatgg agcttgggat
    13081 aaaggtaata tctatcacaa gctcagaaag agtacaactc taaaagaaat atacctagtg
    13141 agatacgcag atgattttaa aattttctgt agaaattata atgaagcgaa aaaagttttc
    13201 tatgcaacta aaatgtggtt acaggagcgt ctaaagttac caatatctga agaaaagtca
    13261 aaaatcacaa atctttcaaa agaagaaagt gaatttttag gctttacgat gaaattagtg
    13321 accaaaggta acaaaaaggt tgttaatacg catataggta caaaagcaca aagcatgatt
    13381 aaagtgaaac taaaaaaaca aatcaaacga attcaaaaaa gtcctaatag caaagatacc
    13441 tttatctata ttggagtcta taacagtatg gttattggga ttcataatta cttcaaaatt
    13501 gcgactcatt gtagtgtagc aatgcgaaaa attgcaagag aactcagacc aagttttgaa
    13561 atacgactac gagaacaagg aatctcaaaa gaaggacaat acggaaagta cgaaggatac
    13621 aagccttacc tacggtcagg acaaatacga tatataagag ggtttccaat attaccagta
    13681 gggtatattc aacacagaca tccgatgtgt aaaaaaagaa atattaataa atacaccgaa
    13741 gaaggtaggg aagccattca taagaagcaa caaaccgtag aagaatggaa gatatcgtgg
    13801 ttgtgtagca atccagtgct tagtaagaga gcaacgattg aatttgcgga caatcgaatt
    13861 tcaaaattca tcgctcaaaa gggaagatgt gcagtaacag gtgaggagct tatcttatct
    13921 gaaatgcatt gtcatcatat tatcccttat catgaaagca aaagtgatag ctatgaaaac
    13981 ctagtgattg ttactgagga ggttcatcgg gtaatacacg ctacacaaag tgaaacgatt
    14041 gaggaacttc taaaatatct aaaattaaat ccaaaacaaa aagaaaaact caatgagttg
    14101 agattaaaag ttggtaacga agaaatatct taataaatat ttgtaggacg ggaaaacaga
    14161 atgtctttta tagacgaggg aacgccgtat gcggtgaaag tcgcacgtac ggtgtggact
    14221 gggggaaaag acggagataa tatcaaactc ttacctatca gtatcgatta atccagattt
    14281 tattgaaacg gtaaattatg tcacaaactt tcaacgagaa atgcgtaaag tagaagcaat
    14341 gactgtttta gctacacaat ccccacaaga aatggtgcca gaaaatatgt cttctgataa
    14401 cttctcgaaa ctgaagaaag tctttgaatt ttcttctacg aagatcttta tgcgtatgga
    14461 tgaatctatt ttacgtcata tagaaggatt agtgggtaaa tcaatgacga actcagaatt
    14521 agaatccatt cctcaacaaa atcaaggaga cgctgtgatc aattttggct caaaagaaac
    14581 cattcgtgtt cattttagtc cgaacaaacg tcaattaaaa ttgtttgatg gaggaaaata
    14641 atggaaaacc aaaatgaaag tttaatcaag cagtatgtta agcgtcgtgc aaagcggcgc
    14701 ttatttttgt ggttgtttgg tacttctgca ggtttgatta ctattttgat cactgtgttc
    14761 gtgaccctat ttctaatact tgcggcgggc tcgattgata attcggattc agattcaagc
    14821 agtggtggcg aagcttttac aggagaatat tcggaaggct taccgattta taaagaaatc
    14881 aaaggaagag gccctttttc tgatgagatt gcgcaatatg ccgtaggtgc agcagtgaaa
    14941 tataaattat taccaagtgt gatcctttcg caatacggtt acgaatcagc gtttggtaca
    15001 tcggcgtccg caagaaatga tctcaactat tttgggatta cttggtttga tggttgtctg
    15061 tttcctaaag gaactgcacg aggaattggt ggcattgaag gtggctggta catgaagttt
    15121 cccaactcaa aagctgcatt ttcctattac ggctttatgg tagccacaca atccaatttt
    15181 aatgcttgtg taggaaataa aagccctggc gcaagcttat tgatcttagg acgaggcggt
    15241 tatgccgctg caggaattac ggaagatagt ccgtattata caggttgtat gtcaattatc
    15301 acttctaata aattaaccga atatgatgaa ttcgccatca aacattgggg tgaaggtggc
    15361 aataacaacg gcacgattac tggtgaatgg acgaatccat tccctggcag cagtttagat
    15421 aaaagttcct tcagcggtgg tcaattattt ggaacgaatc caggcggtga attccgtccg
    15481 aacggcttcc atgatggatt agattttggt tcagttgatc atccaggaag tgagattcat
    15541 gcggttcatg gcggaaaagt cgtgtacgtt ggcaatcctg gtatttcagg tttgggcgcg
    15601 tgtgtgatcg tgattaatta cgatggcttg aatatggtgt atcaagaatt tgcaaatagt
    15661 acaggaaatt ctcgagtgaa agtaggcgat caagtgaaag ttggtcaagt aattgggata
    15721 cgagatacag cacacttgca tttaggattt actcgaatgg attggcgtca agcacaagga
    15781 catgcattca ttgatgatgg tacatggatc gatccgttac catttttgaa tagcagtaaa
    15841 aaatagatca ggagaaaaaa tatggaaaat aaaacgttaa aaatcgtctt aacagctgca
    15901 attagcgtta gtttaatcgg caatattgcg ttgttttggc agctgaaaga ggaacaagca
    15961 gcaaatacct ataaaattga agaagtggct aaaaacgcgt ctgagaagct caaaaaaaat
    16021 tcctctgagg cacaggcgaa gatcgacaag ttaaatcaaa ccatcaatga gaaaaaccaa
    16081 gaaatcgatc agctgaagca acaagatggc acaaaagcaa aagaggaagt aactgaagca
    16141 caaagaaaga cggcagaaga gttcggtcaa ttggccattg ataaatcatt aagcagaaat
    16201 gaattaacgg aaaaactaaa agatgtcgca acccaagaag tgatcgacaa attaagtcct
    16261 acagacgatg atcaccaaca tgatgattac ggaagttact cctttacatt aggtgacgtg
    16321 caaacttacg ctcaaacaag cagtgtaaaa gaggaacaaa attttgtcgt atttgttgat
    16381 tatacaatcg gcaatccgca attcaaagat gtcaaaccac aaaaaataaa aggtggatta
    16441 actgtcactg aaaaacaagt agatggtcaa tggaaagtca tagactttag ttatttcact
    16501 cgttaggagg aagaaaatga gtaagaaaaa aggagaaaga caacgtttca aaaaacgaaa
    16561 aagttatctt agatggctgg gaatctttat gattctcggc tttttgattc ttgctagtcc
    16621 gatttatacg aatcagaagg tagatatacc agatgaggta agcacgaacg cctttttaaa
    16681 cacgaaagag ctgacaatgg tttcaaaaga aaaaggagcg aaccagcaat tagtttttga
    16741 atttgcgatc gatgatgaag atcaaagctt gttaaaagaa ctggctaatc ttaattatcg
    16801 cgtagaagta aagacagcta aaggggatta tcaagcaata cgagccaaag tgattaaggt
    16861 gtctgatgat tattttgcag tgaagctaac gcatgtacca gaagaatata tcgccatgcg
    16921 tttaaccatt attcctgaaa aaattgatcc aaaagtggat atgcaagaac cacaagatct
    16981 gatttattac gtgcacgaag ataaggttaa agatcaagtg aaagatgaag attatgaaca
    17041 acacgcgatc aattacaaag tgaaaggata caagaaagaa caaaaagcag ctcaaaaaca
    17101 aatggaatct ttccaagcaa ccattgattt gaatgaagaa ttagtgaaga agctaaaaga
    17161 tcaattgcct tatcaagtcg cagatgatca ggaaaatact gaaaacaaga ttaatagcta
    17221 ccagcaagaa attgaaacat cgaaaacgca actgaaagat caagaagaga tcattcaaaa
    17281 acttcaagaa aaaattgatc gtatgacaaa agaaaagatt tagaaggaca ggtggagtga
    17341 aaaaatgaag cgaacaaact tggctgacaa agatactttc gctgagcaga aagtattgat
    17401 taaagcctat ctcaaagttg taaaagctaa agaaatatgt cgagaagtga atttatctaa
    17461 cacaacaata tctgagttga agaatggtcg aaagaattta gatactttaa aattcagatc
    17521 atttgaagct cta
//
LOCUS       DAKBVG010000037        17171 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000037 DAKBVG010000000
VERSION     DAKBVG010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 17171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17171
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.037"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(43..852)
                     /locus_tag="OUD09_000915"
     CDS             complement(43..852)
                     /locus_tag="OUD09_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCT9692200.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
     gene            1145..2581
                     /locus_tag="OUD09_000916"
     CDS             1145..2581
                     /locus_tag="OUD09_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HCT9692201.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            2751..3947
                     /locus_tag="OUD09_000917"
     CDS             2751..3947
                     /locus_tag="OUD09_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HCT9692202.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(4009..4194)
                     /locus_tag="OUD09_000918"
     CDS             complement(4009..4194)
                     /locus_tag="OUD09_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HCT9692203.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            4425..7739
                     /gene="dnaE"
                     /locus_tag="OUD09_000919"
     CDS             4425..7739
                     /gene="dnaE"
                     /locus_tag="OUD09_000919"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCT9692204.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLGPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            7907..8869
                     /gene="pfkA"
                     /locus_tag="OUD09_000920"
     CDS             7907..8869
                     /gene="pfkA"
                     /locus_tag="OUD09_000920"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCT9692205.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            8943..10727
                     /gene="pyk"
                     /locus_tag="OUD09_000921"
     CDS             8943..10727
                     /gene="pyk"
                     /locus_tag="OUD09_000921"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCT9692206.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(10762..11664)
                     /locus_tag="OUD09_000922"
     CDS             complement(10762..11664)
                     /locus_tag="OUD09_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCT9692207.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            11850..12419
                     /locus_tag="OUD09_000923"
     CDS             11850..12419
                     /locus_tag="OUD09_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HCT9692208.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDLSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            12477..12656
                     /gene="rpmF"
                     /locus_tag="OUD09_000924"
     CDS             12477..12656
                     /gene="rpmF"
                     /locus_tag="OUD09_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCT9692209.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            12826..14247
                     /gene="gndA"
                     /locus_tag="OUD09_000925"
     CDS             12826..14247
                     /gene="gndA"
                     /locus_tag="OUD09_000925"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCT9692210.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDEAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
     gene            14440..15126
                     /locus_tag="OUD09_000926"
     CDS             14440..15126
                     /locus_tag="OUD09_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCT9692211.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            15123..16628
                     /locus_tag="OUD09_000927"
     CDS             15123..16628
                     /locus_tag="OUD09_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCT9692212.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
BASE COUNT         5555 a         2984 c         3596 g         5036 t
ORIGIN      
        1 aaaaattgtc acaacctctt ttttgtggaa tctaattctc tattatagtt taagtagttt
       61 ttccagataa acagctaaac cgtcttcctg attgctcaaa ggagtgacat catttgcaat
      121 gcctttgagt tggtcagtac cgttagccat agctactccc caacctgcat aatccaacaa
      181 ttccaaatca ttatgctcgt caccaaaggc aatgacgtct ttttgatcga tagacaggta
      241 gttagcaatc tcttgcacgc cttttgcttt ttggattcct tttgaaacaa tctctaagat
      301 ggcagtaggt ccaccccatg tccgaacatc gacataactg ccaaattgac gtgttaattc
      361 agcagataca gctcctgcaa aacgtttgtc tgttcggaca agcaaagatg taggatttgt
      421 cgtcaaattt ttaggagaaa gcaaattctt ctctcctgga cgagaagaag caaaaatttt
      481 ttcatcaaag aaatcaaaac tgtcaataaa aaaagtatca cgattttcag cagcgataaa
      541 atctaaattt agctgctttt tttgcgataa gatttcaaaa gcgatttcac gattgatcaa
      601 cgtttctctt tcaccatccc agtgctgatt agggatgtgt actaatgcgc cgttaaaatt
      661 aaccatcggt gttgttaatt ctaattgttg gtaaaactga tggctcattc ggaatgggcg
      721 tcctgtagca atactgacat gatgaccggc attgattgct ttttttagag tctcttttgt
      781 tttagatgta atcatcgaat cttgattgag ggtagttccg tccagatcga tggcaattaa
      841 ttttttattc aataagggtc cttccttcct caactagcat ttgttttagt ctagcatatt
      901 ttaggtaatt aacaagagca atcagctatt cctaacaaaa actacagtta tggctatata
      961 aatagctaaa cataagctaa acacgaacgt taagtattat tttcgctttt tttgttaaaa
     1021 aaacgttgaa aagcgaatga aacgtctata taatttgaaa ttgttcgatg gatttgccct
     1081 attttactta ttttttatta ttatagtttt ccatcagaaa aaaattcagg gagtggaact
     1141 ttttatggaa aagtttttcg gtttaaagaa aaacaataca aatgtatcaa ctgaaataat
     1201 ggcgggagta acgacattct ttgccatgag ttatatctta ttcgtaaatc cgacgatttt
     1261 gtcagcatca ggtatgccct tccaagcagt atttctagcg acaatcattg catcgattat
     1321 tggtacgctg gtaatgggat tgttcgcaaa tgtaccatat gctcaggcac ctggtatggg
     1381 attgaacgca tttttcactt ttacggtcgt ttttgggctt ggttattctt ggcaacaagc
     1441 cttggcaatg gtgtttattt gcggattaat caatattttt attactgtca caaatattcg
     1501 aaaaatgatt attcgggcga ttcccgaaag tcttcaacat gcgattggtg gcgggatcgg
     1561 tattttcgtt gcgtatgtcg gaatcaaaaa tgcaggattc ttatcttttt cagcagacca
     1621 gtcagctatt tcgagctctg tcgttgaagg cggaaaagcg acaaatgtta cgataaatgg
     1681 tggaatcgtc cctgcgttag ccaactttga caacgctcct atcttattgg ctgtgattgg
     1741 tttggtactg acagccattt tagttgtaaa aaatgtccgt ggggctattc ttatcggtat
     1801 cgtagcgacc acagtccttg gtatttttat gggtgttgtt gatttaagtt ccattgattg
     1861 gcaaacgaat tcgttaggta attcatttaa agaattagga acaactttcg gtgcagcatt
     1921 tggttctgaa ggcatgcaat ctttattcag tgattcttcc aagattcctc aagtattgat
     1981 gacgatcatc gcattcagtt tatctgatac tttcgatacg atcggaacat ttatcggtac
     2041 aggacgccgc acaggtattt tctcaaaaga agacgagatc gcgttggaag atggtcgtgg
     2101 gttcaaaaca aaaatggata aagcactatt tgccgatgcg atcgctactt ctgtgggggc
     2161 tatctttggt acatcgaata caacaactta tgtcgaatct gcagctggga tcggggccgg
     2221 tggacgtact ggtttgactt cagtagtagt ggcagtgcta ttcgcattaa gtagtttatt
     2281 ctctccactg atcgccattg taccaaatca agcaactgcg ccagcattga ttttagttgg
     2341 agtaatgatg atggcttctt ttgcagacat caaatggcta gatatggaag aagctttacc
     2401 ggccttcttt gcatcgatct ttatgggatt gtgctatagc atttcctatg ggattgcagc
     2461 aggatttatt ttctatacaa ttgtgaaagt aatcaaagga aaatacaatc aagtttctgt
     2521 tgctttgtgg atcgttgata ttttattcat tatcaacttc atcattctag caacaattta
     2581 actaaagaga agggattgga acagtatttg ttctgatctc tttttgtttt acataaaaag
     2641 aacgttacat ataaaatgac ctttgcggga tttttctctt tttactggcg agatcgttgc
     2701 ttgtttattc gaaaataggt atgataaaag agacgaaaga gggatgatag atggagatta
     2761 attggagaaa aaatttagtg attgcgtgga ttggctgttt tttcactggt gcaagtatta
     2821 gtttagttat gccattcata ccagtttatg ttgagcaact aggaacacca aaagatcaaa
     2881 tcgaattgtt ttcgggacta gcaatctctg tgactgcttt tgcatctgcc gtggttgcac
     2941 caatctgggg aaatctagct gatcgaaaag gccgaaaact catgatgatc cgtgcagcag
     3001 cagggatgac attaacaatg ggatctttgg cttttgtacc aaacgcgtat tggcttttga
     3061 tcatgcggtt ttggaatggg attttgtctg gatatattcc aaatgccaca gcgatgattg
     3121 cttcacaggc acctagagaa aagagcggat gggcgttagg aacattgtct accggagcga
     3181 ttgctggaaa tctgatcgga ccttcaatgg gcggtgcact ggctcagtgg tttggtatgg
     3241 aaaatgtatt cttgatcaca ggggctttgt tgatgatcac aacggtattg accattttct
     3301 tagtcaaaga agatttccat ccaatcgaga aaaaagatct gataagcacc aaagaaattt
     3361 tttcaaaaat ggatcatctc tctattttga tcggtctctt cattacgacc ttgattttac
     3421 agataggaat cactacgatc agccctattt tgaccttata tattcgtgaa ttgagcggaa
     3481 gtacagaaaa tatccttttt gtcagcggct tgattgtttc gatagctggt gtttccgctg
     3541 tcttttcttc accgaaacta ggaaaactag gggataagat tgggaatcaa aaagttctaa
     3601 ttggaggact cattttatca ttttgctgtt atttacctat ggcattcgtc actacaccgc
     3661 tccaattagg tatacttcgc tttattttag gcttttcaac tggcgctttg atgccttctg
     3721 tcaatacact gatcagtaag attacgcctc aagaaggagt cagccgaatc tatagctaca
     3781 atcagatgtt tagcaacttt ggacaagttt taggtcccat gctaggatcg acagtcgcgc
     3841 atgcttataa ttacagtgct gtttttattg tgacaagttt gtttgttctg tcaaatattc
     3901 ttctttcttt attcaacttt cgtaaagttc ttcgcagcag attataatgg tggtgaggag
     3961 tagaaaataa aaaagagctt gagaaatctc aagctcttgg ctgttttttt acgaatgggc
     4021 agaggtttct tttcttggcg caactgcacc aatcaaaagg ataatgacac ccgcaatgaa
     4081 tgaaatgatt gttgtcaaag taaaatcata tgttccgctt gttaatgcag caccaatata
     4141 accgactact tgacctagaa cgacagccca gaataatgta acgatatatc gcataaagaa
     4201 cacctctttt ctatcttaat tttaccagat tttctttgaa tgtaaagaaa cgagaccaaa
     4261 tctcaataaa atttgagttc ttgcgaaaga actcattcct acagattaca agcaagcaaa
     4321 tgaagtggaa taatcaactt cagcgcctgc tatcttaatg aaagtaaaag gatcaaacct
     4381 tttaacgcta gtgaatttta acgaaaaaaa aggcggaatg caagatgttt ttcccacaac
     4441 tttatacaat tacttcttat tctcttttac aaagtacgat acgtattcga gaatatgtac
     4501 aagaagcaaa aaaacaaggc tacacttcat tagccataac ggataaagat gtcttatacg
     4561 gtgcacctga attctatcgt atttgtgtag aagaaggagt acagccaatc attggattac
     4621 atcttagtta tacttatgaa gaaaaaaatt atggcttgct tgcgtacgca aaagatgaaa
     4681 taggttatca gcagctgatg aaactttcca gccgaaaaat gatccaagaa aaattggaac
     4741 ttgcagaaat gacagaaacc gaccatctag cgtttgttgt tccggcaaat cattctgttt
     4801 ctgaggcttt caataaagag gataactttt tcggtcattg gcagttttta cttgaaaatg
     4861 tttttactag aaatttttat atcggcttga cagaaaacac gcctgatcaa ttgcctgaat
     4921 ggtatcagta tgtgataaaa gaaaattatt cgctttgtgc attacatccc atcgctagtt
     4981 tacaacaaga agaacttttt gcacttgaag tgatgaaaca tatcaaagag ggaagccaaa
     5041 tacctttaga aaatttatct gcatctttac cactaacagc aaatgtctat cttctttcag
     5101 aacaagaaat gatcaaacga ttcgaaaaag cgggtctttc ccaagctgta ataggcgccc
     5161 agcgtttagc tgaatcgatt tcatttgaat tccagttcca tcagaaatta cttccgcatt
     5221 atccagttcc taatggaaaa caagctggag aatatttaag agagttatgc tattcgcaag
     5281 tggaacgccg aatcactgac tatacaaagg aataccaaga acgtttggac tacgagttat
     5341 ctgtcatcca tcaaatggga ttcgatgatt atttcttgat cgtatgggat gtaatggctt
     5401 ttgctcatag aaacaaaatc gttaccggtg caggtcgtgg ttctgctgcg ggttcattag
     5461 tagcctatgt gttagaaatc acagatgtgg atccattaga atatgatttg ctgttcgaac
     5521 gctttttaaa ccccgaacgc tttacgatgc cagatatcga tttggatatt cctgacaatc
     5581 gacgtgaaga agtattgaat tatgttcgag aaaagtatgg acaatatcat atggcacaaa
     5641 tcgcaacatt tggcacaatg gcagcaaaaa tggttcttag agatgcagct agagtgtttg
     5701 gtttatcgca aagtgaagca aaccgctggt caaaagcaat acccaatcaa ttgaaaatca
     5761 cattgaccca agcttataaa acttcgcatc aattagtaga attagttcag gcaagcgaaa
     5821 aaaaccaact tttattcaaa actgcagtga ttttagaagg attgccaaga catgtttcga
     5881 ctcacgcagc aggtgtagtt atcagcgata agaatctact tgaactagtt ccgctccaac
     5941 gcggttctga tgaagctttt ctgacccagt atacgatgaa tgaggtagaa gcaatcggtc
     6001 ttttgaaaat ggacttttta ggactacgca acttatcgat tattgatttt actttgaaag
     6061 ctgttaagcg agtagaaggg tatgagatcc gattaaaaga gatcccgcta aatgatgcaa
     6121 aaacgctgct cttgtttcaa cgaggcgaaa cctcaggagt atttcaattt gaatctgctg
     6181 gtatccggaa cgttttacga agattaggtc cagaaaacat cgaagatatt gccgcagtca
     6241 atgcattgta tcgaccaggc cctatgcaaa atatagatac cttcattgcc aggaagaaag
     6301 gaaaagaagc cattcgctac ccagatgatt cactgatccc gattttgaaa aacacatatg
     6361 gagtgatcgt ctatcaggaa caaatcatgc agatcgcctc gactatggct ggatttacat
     6421 tagggcagtc agatattctg cgacgggcag tgagtaaaaa gaaaaaagac gtattagatg
     6481 aagaaaagtc gcattttgta tcaggagcat tacaacaggg atattcggaa aaaacagcag
     6541 cgaccgtata tgaatatatc gaaaaatttg caaactatgg attcaatcgg tcacatgcat
     6601 ttgcttattc attcgtggga ttccaaatgg cgtatctaaa agcccatcat ccaggtgctt
     6661 tctttaccag cctgatgaat tctgttcggc ataacacatc aaaattgaaa gaatatattg
     6721 cagaagcacg taaaaataaa ctcaaacttt cctcgccgtc cattaatcaa agtaattcca
     6781 gttttgagtt ggtcaataag gaagaaatca gattcggact gacagcgata aaaggtattc
     6841 ggcgtgattt tgtagaagat atattaaacg aaagaaaaca aaatggcccg ttccagtcag
     6901 tggatcaatt cctcatacgg atcgataagc gttggctgaa attagagtta ctgcaatcac
     6961 tgacagcagt aggcgttttt gatgaattat ctcctaacag gaaacaattg atgcttgatc
     7021 tcgaaggaaa aatccaaaat atcgtttaca gtggtggaag tcttgacttg ctgggaatca
     7081 tggcattaaa agaagaagaa gtcgctgact atacactaga agaacgcttg aagctggaag
     7141 aggagtactt aggtatttat ttgtcaggtc atcctgcaga agggtttgat cgtcttcgtt
     7201 tagctaaaca agttcagctg attacagata tcgtccctaa tcaaccagtc aatttattag
     7261 tgatgatcaa ggaagttaga gaaattcgga caaaaaaagg agaactgatg gcctttgttg
     7321 aaggaaccga cagtagtgga gaaatttctg tcacagtttt tccaactctt tatcgtaaat
     7381 atcgtaattt gtttgccgtt gatcgagtga tctttgttca agggcggagc gaaatcagca
     7441 aatataatca ggaactgcaa ctaattggag aaagctttgc tgatcctaac cagttagaag
     7501 cccagtatcc ttcacaaact tgctatttgc gtattacaga agaacgggaa gatcccgaga
     7561 taatgaaaca attacaagtg atttttcaga aacacccagg atatatacca gtagttatgt
     7621 atcacgaaaa aacgcaacga aaagtagttc tgtcagatgc ttattgggtg gatgacagca
     7681 agagtataaa ggggcaactt atttatctgc ttggcgaaga taatgtggtt ttcaaataac
     7741 ttacggtaaa aaatactact tgctttcatt ttttttcatt tttttatccg taaatcaaga
     7801 caagtgagaa aaataatgtt agaatgttag tcgtggttaa cgcaaaaatg gttgtaaacc
     7861 taagtcatac ttaggataaa gtattttaat gaggtgaatg tttattatga aacgcatcgg
     7921 aattttgacc agtggcggag acgcacctgg aatgaatgca gctgttcgtg ccgttgttcg
     7981 taaaggtatt tacgaaggac tggaagtata cgggatcaat tatggttttg ccggtttagt
     8041 tgctggagat attcgtcgtc ttgatgtggc agatgtaggt gacaagatcc aacgtggtgg
     8101 tacattcttg tattctgcac gttatccaga atttgcaact gaagaaggtc aattaaaagg
     8161 aatcgaacaa ttgaaaaaat tcggaatcga aggtctagtg gttatcggag gagacggctc
     8221 ttatcatggc gcaatggctt tgacaaaacg cggtttccca gcagtaggta ttcctggaac
     8281 aatcgataat gatattcctg ggactgattt cacaatcggt tttgatacag caatcaatac
     8341 agtactagaa tcaattgacc gtattcgtga cacagcgact tctcatgttc gtacattcgt
     8401 tatcgaagta atgggacgca atgcaggaga catcgcttta tggtcaggtg tggctggcgg
     8461 agcggatgaa atcatcatcc cagaacatga ttttgatatg gcttcagttg ctaaaaaaat
     8521 ccaagaaggc cgcgatcgcg ggaagaaaca ttgcttgatc atcttggctg aaggtgtaat
     8581 gggcggaaat gaattcgctg agaaactttc agaatttggc gactatcata cacgtgtatc
     8641 tatcttggga cacgttgtcc gtggtggagc accaagcgca cgcgaccgcg tgttggcaag
     8701 taaattcggt gcttacgctg tcgaattgct tcaagaagga aaaggtggat tatgtgtcgg
     8761 tatccatgac aacgaaatcg ttgcgtctga catcattgat acacttgaaa acaacaaaca
     8821 taaaccagac ttatcactat atgatctaaa taattcactt tcattttaag agtgaaagaa
     8881 accaagctat ttaagctgtg tacagcttta aataaacaaa attgaatggg agagtttttt
     8941 aaatgaaaaa aaccaaaatc gttagtacat taggaccagc aagtaactca gtagaaatta
     9001 tttctcaatt gattgaatct ggggcaaatg tcttccgctt taacttttct catggtgacc
     9061 atgaagaaca attatcacgt atgactatgg tccgtgaagc agttaaaaag acaggaaaag
     9121 atgtcggtat tcttttagat acaaaaggag ctgaaatccg tacaactgtt caaggcacaa
     9181 cagaagctga ttttggtcgt gcaggataca tcaaatttga agtaggagat caaacacgca
     9241 tctctatgga tcctgaacat gtaggttcaa aagaaaaaat cgcggttact tacccaggat
     9301 tatttgacga tgttcacgtt ggcggacaca ttttatttga tgatggtttg atcgatatgc
     9361 aaatcattga aaaagacgaa gcaaaccgtg aattagtcgt agaagttaaa aatgcaggta
     9421 tgctaggatc acgtaaaggg gtaaatgcac caggcgtttc tattagtttg ccaggtatta
     9481 ctccaaaaga tgctgacgat attcgttttg gtttagacaa cgatatcgac tttatcgctg
     9541 caagttttgt acgtaaagca caagacgttc tagatatccg cgaaatctta gaagaaaaag
     9601 acatgactca tgttcaaatc ttccctaaaa tcgaatctca agaaggtatc gacaacattg
     9661 acgaaatcat caaagtttct gacggtgtta tgattgcccg cggagatatg ggtgttgaaa
     9721 tcccagctga actagtacca atggttcaaa aacgcatcat caaaaaatgt aatgctgctg
     9781 gtatcccagt tatcactgct acacaaatgt tagaatcaat gcaagaaaac ccacgtccaa
     9841 cacgtgcgga agcttctgac gttgctaacg ctgtatttga tggcacagat gcaacaatgc
     9901 tatctggtga atctgcaaac ggggactacc cagttgaagc tgtttcaaca atggcacgta
     9961 tcgaccaaga agctgaaaat gcattatcag aattaggtac attccaattg aacgaattcg
    10021 ataaaacaga tgtaactgaa acaatcggtt tatctgtagc tcgtgctgca aaaaatctag
    10081 gcgtgaaaac aatcgttgct gcaactgaat caggatacac tgcaaaaatg atttcaaaat
    10141 atcgtccaga tgcagatatc ttggctgtta cttttgacga acgcacaaaa cgtggtttga
    10201 tgttaaactg gggcgtttac ccaacagttg ctgaaaaacc atcaacaact gatgaaatgt
    10261 ttgaactagc agctaaaaaa gctgttgaat taggtttcgc atcagaaggc gatttaatct
    10321 tgatcactgc tggtgtacca gttggtgaac gcggaacaac taacgtgatg aaaattcaaa
    10381 tgattggttc taaattatta gaagctcaag gcgttggaca aaaatctgtc gttgcaaatg
    10441 cagttattgc ttcatctgct gaagaagctg taaaaaaagc aaaagacgga atggttctag
    10501 ttgtaccatc aactgacaaa gaatatatgc ctgcaatcga aaaagcagct gctgttatcg
    10561 ttgaagaagg cggattgact tctcacgctg cagttgtagg aattgcacaa gatattcctg
    10621 taatcgttgg tgctaaaggc gcaacaacaa caattacaga aggtgaattg attaccgttg
    10681 attcacgtcg cggcatcgtt taccgtggcg aaacaacagc tatctaattt agcttaataa
    10741 aaaataaaaa cagctcttct tttacgaaga gctgtttttt tgtgctgtaa cgaattcatg
    10801 gataacttgg actacttgtt cttgaagagg cgtgacgatc tgctgtgttc gttttgccaa
    10861 tgaaatcaag aagcgaggct gaacttcatc tgaaagaggg aggcaaacta aatgatcttc
    10921 aggatggaca gcaatctctg ccaaaaaacc gatacccaca ttttctcgga ccatactctt
    10981 taaaatcgtc aagtcattgc tttgataaac aacttttgga tgagtatgtg tttgtttaga
    11041 aagcttagtg aatgctgcgg ggtggacata atgttcgttc aatagtacaa actgttcatc
    11101 tgctatatct ttgaaagaga cagtatcctg cttggaaaga ggatgtgtag gtggcacgac
    11161 gatcatgaaa cgtttgtcca aaagtaactc tgtttctaaa tattgatcca cgattggctg
    11221 tgttgagcct aatacagcta aatcgatcct cccttgtcgg atcatcccgt ataagtcccg
    11281 agagcctcca tcggtaatct caatttgatt catcaattca tgcttgataa agtaacttga
    11341 cagtttagga aagtattcat tgccgataat aggaggcatg ccaaaacgaa gctttttctt
    11401 ttgcaactga gtgatttcag ctcttgcagt agaaatctct tggagaatgg tttgtgcatg
    11461 gagaaacagc tgctttccag aaggcgtgac gattaattcg tgatgcgatc gatctcttgt
    11521 aataagtacg gtgtcaaact ctttttcaag acgttgtaat gcatatgtaa tagtaggttg
    11581 gctcacacgg aaataattag ccactttcgt aaaatttttt tgtttgacga tttggacata
    11641 gtattcgata tctttcgtgt tcatgaagat catccgctcc tttaatggaa taatgattat
    11701 tgtaaatgat tctgaggatg atagcaaaca gaggctttct aataaagatt tttctgtttt
    11761 tttatgaact gcaaggtgtc aagaaaattt cattgacaac atgctttttg ccctgtataa
    11821 ttgcttttgt tgcttaggag tgatgaaaaa tgaaatggtc tttattggaa ttgagaaaat
    11881 accaggagac accgttaact tttcatgaga cgctcgactt gaaagccgct ctcatgaagc
    11941 gagacaattt aatacttgat gttgcgccag tggaagtcga aggtttggtc tcagttgata
    12001 agaaaggcta tatcgttcat tacactgtgc agacgacttt gacagttcct tcaacacgtt
    12061 cactagaacc tgttgaactg cccatggatc tttctgtaga cgaagtattc atgacagaag
    12121 aacaattcca aagtagagat gaacaaatgt ctgcggatga gattttgcta attgaaggtc
    12181 cattattgaa tttagcagag tcgattgaag acaacatctt attggcgatt ccaatgcggg
    12241 tattgacaaa agaagaggaa gcagcaacag aacttccaaa aggcaatgat tgggaagttg
    12301 tttccgaaga agaataccag cgcagaaaaa tggaagaagc cgagaataaa attgatcctc
    12361 gtcttgcaaa actatctgaa ctttttgatt ctaaatcaaa agacgacgat aacaagtaag
    12421 attggaatat attgtgtcta atttagatac aagactttac aaggaggtgt agcaaaatgg
    12481 cagtaccagc tagaagaaca tcaaaagcta aaaaagcaaa acgtcgtaca cactacaaat
    12541 tgacaatcaa agggttaaac gaatgtccaa actgtggtga aatgaaaaaa agccatcacg
    12601 tatgtgcaaa ttgtggttac tatgatggta aggacgtaat gtcaaaagaa gcttaataca
    12661 ttttaattag aaaatgtaaa acctcaaggc taaccatgga gccttgaggt ttattttttt
    12721 gtagtcggta aataaaaaat atgttgttct ttagtttgca atgtagccgt gttaagctat
    12781 aatgtaagag attagggaga aatcccgtat gtaggaggag ttcatatgtc aaaacaacaa
    12841 atcggcgttg tcggtatggc cgttatgggt aaaaatctag ccttgaacat agaaagccgc
    12901 ggctattcag tggctttatt caatcgtaca ggggctaaaa caaccgctgt agtagaagaa
    12961 cacccagata aaaatttcaa agcaacatat acaatcgaag aatttgtaga atctatcgag
    13021 aaaccacgtc ggatcttgct aatggtcaaa gcaggaccgg caacagatgc aacgatccaa
    13081 gagttgcttc ctcaccttga taaaggcgat attttgattg acggaggaaa tacattcttt
    13141 aaagatacga tgcgtcgtaa tgaagagtta gccaactctg gcatcaactt tatcggtaca
    13201 ggcgtttccg gtggtgaaga aggagcttta aaaggacctt caatcatgcc tggtggacaa
    13261 aaagaagcct atgcgctagt tgcaccaatc ttagagcaaa tttctgcaaa agcagaagac
    13321 gaagcaccat gtgtcacata tattggccca aatggagccg gacattatgt gaaaatggta
    13381 cacaacggta ttgagtatgg agatatgcaa ctgattgctg aatcttatga cttaatgaag
    13441 aatattttaa acctttcagt agaagaaatg gctgatatct ttaaagaatg gaatcaaggc
    13501 gaattagaca gctatttgat agaaatcact gctgacattc tgacacgtaa agacgatgaa
    13561 ggaacaggta aaccagtagt agatgtgatc cttgatgcag ctgggaataa aggaacaggt
    13621 aaatggacaa gccaaagcgc gcttgactta ggtgtgccac ttccattgat cactgaatct
    13681 gtatttgccc gctatatttc tgcttataaa gaagaacgcg tacaagcaag caagatttta
    13741 tctcgtacga acgattttga gttcacggga gacaaaaaag aattggtcga aaaaatccga
    13801 gaagcgttgt atttcagtaa aatcatgagt tatgcccaag gatttgctca attacgtgtc
    13861 gcttcaaaag aatttgattg ggacttgcca tttggtgaaa tcgcaaaaat ctggcgcgct
    13921 ggatgtatca tccgagctcg attcttgcaa aaaattacgg atgcctatga caaaaatcct
    13981 gagatcgaaa atttattgct agatgattac ttcgttgaaa tcacgaaaaa atatcagcaa
    14041 tcagtcagag acgtcgttgc cttggctgtc caagctggcg tacctgtacc gactttctca
    14101 tcagccatcg cttacttcga ttcatacaga gcagaacgtc tgcctgcaaa tataatccaa
    14161 gcccaacgcg attactttgg cgcacatacc tatgaacgtg tcgacaaaga aggaatcttc
    14221 cattactcat ggtaccatga agaataatag tctttcggct gttaggaaat gagaaaataa
    14281 aaagaggacg aaagagctga agtcgattgt tatgagtaga cttcagctct tttttaaaat
    14341 tgctatccat gataaaatga ctatagattt ttaagcatat tatcggtata atgatagagt
    14401 ttttaaaaag ccagtagaat agaaaggata aaataataaa tgagcaatat tctaatcatt
    14461 gaagatgaaa agaacttggc acgatttgtc gagctagagt tgaagcatga agggtataac
    14521 gcagaagtcc actacaacgg ccgaactggt ttagatgcgg cattaaataa tgagtgggat
    14581 gccatattgt tggatctgat gctcccagaa ttgaacggtt tagaagtctg cagacgtgta
    14641 cgccaagtca aaaatacacc aatcattatg atgactgcaa gagattctgt gatcgatcgt
    14701 gtatctggac ttgaccacgg agcagatgac tacatcgtca aaccatttgc tatcgaagaa
    14761 ttattagcac gtcttcgtgc attgttgcgt cgaatcgata ttgaaggcga taaaaatgtg
    14821 gctaaacaaa ctactattac ttatcgcgat ttgactatcg aaaaagaaaa ccgtgtcgta
    14881 cgtcgcggaa atgaagtaat tgaactaaca aaaagagaat acgagttgct gcttacttta
    14941 atggaaaacg tgaacgtggt attagcacgt gacgttttac taaataaagt gtggggatac
    15001 gaaacagaag ttgaaacaaa cgtagtggac gtatatattc gttatcttcg caacaaaatt
    15061 gacgttcccg gggaggaaag ctatatccaa actgtacgcg gaacaggtta tgtgatgcgc
    15121 tcgtgataaa gccgaaaaaa tcggaaaaaa gagaactgaa agggccctcc ttgacaatca
    15181 agtgggcctt tgcgagttct ttcttcatat tcgttgtgtt tacaattttc gctgtcatta
    15241 cctataaatc atctatcaat ctgattgtgg caaaagaaag aaacaatgta gagcgtacga
    15301 tttcagaagt agcttctcgt ttggctaact cggatgaaga actgacatta gtaaatacgt
    15361 atcgtaatct gacgaaagcg acaaatgacg gagatgaggt cgtagatcat acttcactgg
    15421 tcgaaggaaa cttaatgaag atcgattcct ttatagctga gctagggcag ccatcaatgg
    15481 atctttatgt ttatgatttg gacgagaact tgatttttaa aacccatgaa aacagtcgaa
    15541 acctgattca gactacacgt aaagttccaa cgatcgttac actagaagga aaaactggct
    15601 ttttatctat ccagcctata tattctaaag gtacaagaga aaagatcggc tatgcgcaaa
    15661 gcttctatga attatcttct ttctatgata ttcgaaataa gctattattg actcttattg
    15721 ttttagaagt tgtttctttg atcttaagta gtattttagg tttcttttta tcttcttatt
    15781 tcttaaaacc actcaaggta ctcagagata caatggatac tatacgtaaa gatccgcaat
    15841 cagatatcca catgcctgaa atcgacacaa atgatgaatt ggcagattta gctgaaatat
    15901 tcaatgagat gcttgaccgt atgcgtttgt atattgaaca acaagagcaa ttcgtggaag
    15961 acgtatctca tgagttaagg acacctgttg caattattga agggcatctt agtctgctaa
    16021 atcgttgggg gaaagatgat cctgagatat tagaagaatc attaagcgcc agcatgcaag
    16081 aaattagccg tatgaaaagc ttagttcaag agatgctaga cttatcaaga gcagaacaag
    16141 tagatgttca ttatgcgaat gaaacaacta aaggaaaaga agtcacctat caagtttata
    16201 ataatttcaa gatattatat ccagactatg tgatcacttt agatgatgat ttgacaaaag
    16261 aagtgaccct gcaaatttat cgtaatcact ttgaacaatt aatcattatc atattggata
    16321 acgcagtaaa gtattcaact acacgaaaag aagtccatat ctctatttct tccactctga
    16381 atgaatttga gatcgcgatt caggactttg gcgaaggaat acccaaagaa gaccttaata
    16441 agatcttcaa tcgtttttat cgagtggata aagcaagagc tagaacgaag ggtggaaatg
    16501 gtctggggtt gtctatcgct aaacaactgg ttgaaaacta taaaggacgt atcttagcag
    16561 aaagtgtagt agggcaaggg acaattttta gaatctttat tcctatcgca aaggcagaag
    16621 acaagtaaag catgtgcttt acttgttttt ttatatatcc aacaaagaat aaggtacaaa
    16681 atacaagcaa gttagaaatt cagggatgtc attataaaaa aaactagcga acaattaaat
    16741 agaataacat ccgttaatta aacgtaagta gcttttagat gaacgaaaaa acaatgaaat
    16801 gctgtaatat tcgtgtttaa aaataataaa taaaaatagg gaattttcta ttgcaatttt
    16861 ataaagagat tgctatatta ttatttgttc taaatgattc ctttaaggtg tcactaaaaa
    16921 ttataaaaaa tagggttgac gcacttttga tgtcatgtta taataacgaa gtcgtttgga
    16981 tgaggtagct aataaagctt gattttatcg aaaaaaactt cgaaaaagtt gttgacaaat
    17041 aacaataaag atgatatgat aatcaagtcg ctgctaatga gtgacaaaga cgtttagaaa
    17101 aaataaacaa agaagttgtt gacattctct tctgaatctg ataagattaa agagttgttg
    17161 aaacaaaaag a
//
LOCUS       DAKBVG010000038        17025 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000038 DAKBVG010000000
VERSION     DAKBVG010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 17025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17025
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.038"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            152..1153
                     /gene="ccpA"
                     /locus_tag="OUD09_000928"
     CDS             152..1153
                     /gene="ccpA"
                     /locus_tag="OUD09_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HCT9692213.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLADRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEGFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(1490..3835)
                     /locus_tag="OUD09_000929"
     CDS             complement(1490..3835)
                     /locus_tag="OUD09_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HCT9692214.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            4114..5016
                     /locus_tag="OUD09_000930"
     CDS             4114..5016
                     /locus_tag="OUD09_000930"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCT9692215.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQIFDPEVFPELLAEEVIL"
     gene            5229..7004
                     /locus_tag="OUD09_000931"
     CDS             5229..7004
                     /locus_tag="OUD09_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HCT9692216.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSFYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(7070..8656)
                     /locus_tag="OUD09_000932"
     CDS             complement(7070..8656)
                     /locus_tag="OUD09_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCT9692217.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQEMVEEIE"
     gene            8907..9131
                     /locus_tag="OUD09_000933"
     CDS             8907..9131
                     /locus_tag="OUD09_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HCT9692218.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            9272..11023
                     /locus_tag="OUD09_000934"
     CDS             9272..11023
                     /locus_tag="OUD09_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCT9692219.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSVKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIISAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            11023..12807
                     /locus_tag="OUD09_000935"
     CDS             11023..12807
                     /locus_tag="OUD09_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT9692220.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            13133..13984
                     /locus_tag="OUD09_000936"
     CDS             13133..13984
                     /locus_tag="OUD09_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCT9692221.1"
                     /translation="MLIEKVLNNNVVITRDERDREMIVMGKGLAFKKKVGDPILDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVHLNDSVYISLTDHLY
                     TAIERQKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GAKSEDGEVSEELFQVVQKKYKDTFGCVEKIADFLTGAFDYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            14081..15961
                     /locus_tag="OUD09_000937"
     CDS             14081..15961
                     /locus_tag="OUD09_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCT9692222.1"
                     /translation="MGKYQELAENIVKNIGGKENIRSLTHCVTRLRFKLKEENKAKDD
                     VLKKMEGIVTIMKSGGQYQVVIGNHVPDVYAEVLTVAGISDEVEEEVSSSSNLFNRLI
                     DILSGCFQPFLGALAAAGMVKGLNALFLFLKFYTEGSGTYIMLNGIGDAIFYFMPVIL
                     GYTAAKKFHLNPMVGLVIGAALCYPTVQGAALQSGFPDGSPAPYSLLGLPAYETFLGI
                     PWVGSSYTSSVVPVIFIIAFAGQVQKIAKKWIPAVVQTFLVPFSVLLIALPAGFLIIG
                     PIVSILTDLLSTGFQALMDFSPAVYGAVLGFLWQVLVIFGLHWSIIPLAIMQLAQNGW
                     SQMLVGVYGASFAQTAVVLAMYFKFKDQKMKALCMPAVISGIFGVTEPAIYGITLPKK
                     KPFIYSMIGGAISGIYLMASGTISYTMGGLGIFGVVNYITETDASGMFQAFIGIAIAS
                     VIGFSLTFFFWKDEAGETDAIQKKSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGRGI
                     LIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSETGLELLIHVGLDTVQLEGKYFESLVQ
                     QGERVKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGETVGPDDALITA
                     LT"
     gene            complement(16001..16903)
                     /locus_tag="OUD09_000938"
     CDS             complement(16001..16903)
                     /locus_tag="OUD09_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCT9692223.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKYLLLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENKVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
BASE COUNT         5298 a         3070 c         3551 g         5106 t
ORIGIN      
        1 aaagaccaac gttctaaaaa ctgaaaaaag taaaaatttt catgaaaaca gagataaaac
       61 ggttgcaatt cttgagatta tttgatattc tattttagaa atgtaagacg tattttcaga
      121 aaacatataa aatatgaatg ggagaacaca aatggaaaag caaacaatca ctatctatga
      181 tgtagcgaga gaggcaaatg tttcgatggc tactgtttca cgtgtcgtaa atggcaatcc
      241 aaacgttaaa ccagcaacaa gaaaaaaagt cttggaagtc atcgatcgct tggattatcg
      301 tccaaacgca gttgcgcgtg gcttggcaag taagaaaaca acaacagttg gggtaatcat
      361 ccctgatgtc agcaatatgt tctttgcttc tttagcacgt ggtatcgatg atgtggcaac
      421 aatgtacaaa tacaatatta tcttagcaaa ttcagatggc aacgaccaaa aagaagtcaa
      481 tgtattgaat aacttactgg ctaagcaagt ggacggcgtg atcttcatgg gtcaccatat
      541 cacagacgaa atccgtggag aattttcacg ttcaaaaact cctgttgtat tagctggctc
      601 tattgatcca gatgaacaag taggtagcgt aaacattgat tatgtatccg caacaaaaga
      661 tgcagtaaat aaattggcaa aaagcggcaa taaaaaaatc gcttttgtca gtggtgcgtt
      721 gatcgatcct atcaatggac aaaatcgtct gaaaggctat aaagaagcat taagcgaaaa
      781 taacctacca tatagcgaag gattgatctt tgaagcacaa tacaacttca aagatggtct
      841 atctctagcc gatcggatcc ataatagcgg tgcaacagct gtctatgttt cagatgatga
      901 attagccatc ggtattttgg atggtttatt agatcgtggc gtgaaagtac cggaaggatt
      961 cgaaatcatc acaagtaaca attcactact gacagaagta gctcgtcctc gtttaacaag
     1021 tatcacacaa ccattatacg atttaggggc ggtatctatg cgcctattga cgaaaatgat
     1081 gaataaagaa gaagtcgaag aaaaaacgat tatcttgcct tatggaatcg aagaaaaagg
     1141 atctacaaaa taataaaagg acaaccattc agaggttgtg agcctgccgt ttaactctgc
     1201 gttacaagga gcaatttcaa aaaacagcat ttcatgtttg atgaatattg tgactcgtga
     1261 ttcggagttg gcaggagaac accgtttatt cgggaaaagg ggttatgaca aaattttgtt
     1321 ataatccttt ttttgtgtct taaaaaagag agaaagtggt tgaaattaca tttatcgtat
     1381 ataaaacaat ccgtgtatag atgctgcatt ttgttcacca ccatcaaaaa aataagggac
     1441 tgtgacaata ctattgtcac aatctcttat tactgtgtat tctattaaat caatcatctt
     1501 tctcatctga ttctttggtt gcttgattcg ctcttgcata agctgaagcg gtgttccagt
     1561 aatctgtata gttttcatcg gtaccgccaa cgccaaattt aaaggcgctt ttcttcggtc
     1621 catttttcgc ccatagcgat tcaactgttt ttccaggtgt ttggatggat tttttattga
     1681 cagtaacttt tccaggtgtt tgaccagtaa atccagatac ttttttcttc atcacatcag
     1741 atgataattc gaatttctca tcagtgccaa aaatgtcagg ttttgcttgg taagcttgat
     1801 tgatcagatt ggcaagataa ttagccgaac gtatccttgc tttgctgtcc attccagtat
     1861 tgtcatcgtg ccctgcccag ctactgatgg taatagacgg agtagagaca attagccatg
     1921 aatctcggta ttcattggta gaaccagttt ttccgaccca atctgctttt cctagatttc
     1981 cgtttaagct tgagatggca tctttgaatg gtgtagtgat ttttgcattg atcacactgc
     2041 gcatcatgtc attcatgatc gatgccgttg cttccgaata gatccggaca gagttgcttt
     2101 cgtgtttata aattacattt cctgcgttat ctgtgatgga atcgatgata tagccttctt
     2161 catagacgcc gccgtttgct aaagcctgaa atccattggt ctgttgaagc gtcgtgacat
     2221 ctactgtacc taaaggagca gattcaacgc cccaattgtc atttgccggg tagttcatct
     2281 ttgccaagta gtgctcgtaa gcatattgtt tcgatccacc ggaattcaac acatcttgat
     2341 ataagtgata agccgggata ttgttggacc attctagtga ctcacggacg gtttggaagg
     2401 tgttcgatcc ttcgttagta gcattgacga ttttctcgcc agcattttct ccttcttgcc
     2461 aagttgttgc ataatctgat acacgggatt ctgatccgat aagccccatg tcgattgcag
     2521 gtccataaac aagaactggt ttgattgatg agccagcttg acgttctgta tcgaaggcat
     2581 ggttattttg attctgactg taattccgtc cgccaacgaa tccgtagatg cggccagttc
     2641 tgttatccat caatacattt ccggtttcta cttgactgcc agcaccatca tctaacatgt
     2701 atccataatt ttctacgcca ttttgcaatg cagcatagac atctttatcg attgtcgaat
     2761 ggattgtata tcctttattt tggattgttt gctgtgcctt ttgataataa gcgtcactga
     2821 ccgatgaatc agacatatca gcgttgtctt tttcagccaa ttgttgtgca atgattcttg
     2881 ttgcttcatt catcacagta taatacaaga attctctgtc agtctgttct gcgatttgtt
     2941 gtggtagaaa gtcttttgta aggtcatagg cttttgcttc ttcgtattgt tctttcgtaa
     3001 tttggccttc acgatacata ctgaataaaa caaaatcttt tctggcaagg ccggcagaaa
     3061 gatcttcttt tagcgcacct gtgttcgtgt atggactata cgtgatcggg ctttggggta
     3121 atccagcgat ataagcagct tgcggcagcg tcacatcttt tgcatctaca ccaaagattc
     3181 ctcttgcagc ttcctggact ccagcaatgt tttgtccctt attattacga ccaaatggtg
     3241 agacattcaa atacgtagca atgatctcat cttttgaaaa gtatttttct acttgtgcag
     3301 aaagaatgat ctcgtttgct ttacgtttga atgttgtttc atctgtaagg atctgctgtt
     3361 tgactaattg ctgtgtcaaa gttgatccac cagaagagcc aataccagta gcttcagata
     3421 ctaacgctcg taaaacagct ttaggaacgt aaccttggtg tttatcgaag tattcatcct
     3481 ctgtgctgat aattgcagtt ttcaataaag gtgaaatttt atcagatgaa atagtcgtgc
     3541 gcatcagatc tgtctggatt tttgatatct ctgtattatc tgcataaaca atctttgacg
     3601 tttctgtaat gtttcctagt tctgtctgca gtgctttttt agtcggcaat tgtgtgtctt
     3661 cgacgagata ggcaaaatag ccagcgccta ttccaatacc tagagctgcg aacatcaaaa
     3721 gaagaacagt aataaaaaca gtcaatgatt gaaaaactct aaaaataatc ttaggaacaa
     3781 accatttttc tttttttctt ctattctgtt tttttcgagt atttttgcgt ggcaaaaggg
     3841 tcacctcaac taatggtttt atttcgctaa ttatagcaaa taatagaaca aaaaaacaga
     3901 caaggctgtt tttggcagga atttcttttt taaaggagtt aaaacgtgag aaaatgatct
     3961 tttttataaa agagttctta agcttttctt tagagaaaat ttcagttact atggagaaca
     4021 gggcaaagaa aaatcaatca ttttctttgt atatcatctt accaatatat aggaattgtt
     4081 agtaacaatt gcctttcaag gaggaatcca tccatgttat taggctcaca cgtcagcatg
     4141 agcggaaaga aaatgctgtt aggagcagca gaagaagcag ccagttatgg atcttcgact
     4201 tttatgatct atacaggcgc ccctcaaaac actagaagaa aagccatcga agagatgaat
     4261 attgatgccg gaaaagcgta catggcagaa aacggtctaa caaatatcgt cgttcatgcc
     4321 ccttatatca ttaatttggg gaatacgatc aaaccagaaa tgtttccttt tgccgtccaa
     4381 tttctgagag aagagattgt tcgtgcggaa gctttaggcg ctactcaaat tacacttcac
     4441 ccaggtgctc atgtaggggc aggaacagaa gccggaattg ctagaattat tgaaggcttg
     4501 aatgaagttt tgacaaaaga acagacggca caaatcgctt tggagacaat ggcaggtaaa
     4561 ggtacggaaa tcggtcgaac atttgaggaa ttagcgcaaa tcatcgatgg agtaaaattg
     4621 aatgaaaaat tatccgttac ttttgacacc tgtcatacga atgatgccgg ttataatatt
     4681 aaagaagact tttcaggtgt aatggaagag tttgataaga tcatcgggtt agatcggctt
     4741 aaagtactcc atatcaatga ttctaaaaat ccgcaaggtt cgcataaaga ccgacacgcc
     4801 aatattggtt ttggaacaat cggattcgac acgctgaata agatcgtcca tgatccaatg
     4861 tttgacaata tttcgaaaat cttggaaact ccctatgtcg gcaaagataa aaagaaagct
     4921 tacgcaccat atggaaaaga aatcgccatg ttcaaagcac aaatattcga tccagaagtg
     4981 tttcctgagt tattagcaga agaagtgata ctctaagatt ttctatctga cagcagcaaa
     5041 aaaggaccag gcacattgcc ttgtcctttt ttgtttaaga aacaaaggag tgctttttgc
     5101 agtctttttc atcatgcatc ttctcccccc tctttttaaa tttagacaaa cagggtatac
     5161 tggtagaaaa gtaaaaaaac gaaaagtttc acaagcaagt ggcaaaacgt gattggaagg
     5221 acgaaaagat gctgaacact ttaaaaccct tgatcaacca actaaaaaag aaacatacat
     5281 actttattct tggcatagcc ctattattta caattattcg tttgtattta tatagtaaag
     5341 caatctatgg aacagacatg tcggcaacct atgatgacca gctgctgatt cgctatgctc
     5401 aaaatatttt ggacggtaaa tggctagggg agtatacgac tacaacactt tccaaaggaa
     5461 tctcctacag cttattttta gtacttgcac ataaactgca tctttcttat gcagttttgt
     5521 tcggcatgtt cagtattttt gccagtatat tggttgcact cgcgtttaaa ccgatcgtga
     5581 agaacaagta tcttctttta agtttctatt tttatttttt gttttctccg attagtttta
     5641 cacatgaata ttccactcga acgtatcgta atgccctcgt gattccggct gttgagatca
     5701 ttatcgcttg tttactagct atttattacc gcaaatacgg ttctagcaga aaattgctgc
     5761 catggttttt agggctttct gtcgcttttc ctttcttttg gttcattcgc gaagattcta
     5821 tctggcttct tccttttact gtagtagctt tgggtatcag tttcgtccag atcttgctag
     5881 ggcaagactt ttctttcaaa gaagtaaaga agttaaaaaa acgactcttt cttctctcga
     5941 aacgggaatg gctgaaactt tcattgtttt tacttcctat agttggcttt tccatcgtct
     6001 atcagtggat cgaatataaa aatgagacaa catacggtat tgcaaccatc aatgaccgtt
     6061 ccagtggaga atttggacgt ttgatgaaac aactgatccg aatagatgat gaaacgaact
     6121 tgaataaaga aaacagtgag atatgggtct cacatgctgc actaaataaa gcattagccg
     6181 catctcctac attccatact gtttctaccc gtattgacga attatatacg actcacgcgt
     6241 ggactcaagg tggaaaagta aaagaattac ccggagatat catcttttgg gcattgcgag
     6301 atgtggtaag cgaatctggt tattaccaaa acaatgcaaa agaaaccaat gatttttgga
     6361 aaaaagtcaa ccaagaatta gaagaagcct atcacaaggg aaccatcaaa aagaaaaaag
     6421 agttctatct gatgggatca ggagatggaa agacgatgag tgatctacca atcgtattcg
     6481 atttccttca atcaggatac gcttatgcat ggcgatataa agatttccat caaggaggaa
     6541 attatagttt cggaacgcaa gaacaagtca aacaggctca agaattatta aaccttccgc
     6601 taatggagaa ttggatcgat agtaatgaac cgagaacacc taatttccaa ttaacaaaat
     6661 cagcaaaagt cgcgaatgtc atcataaaaa tttaccaagt tctggctatt cccgtgctga
     6721 ttgcttcaat agcagggata ttacttatga tactaggaac cgtatttgca aaagctgatc
     6781 gtgcctatta tagttcctct ttgattgtgg tcaccggatt aatactgaca caattggttt
     6841 ttcttttcgg tgtctcgtgg ttttgttctt atgcgccaga aaaaaaggat tattttttaa
     6901 gtgtctacac tggcgcaggc gtgccgttaa cgcaattcat cgtagtaaca agcatgctag
     6961 ttttcagtaa attaaatctg aaaaaagctt tatcttctaa ataaaaagac ccttggtagc
     7021 tagattttta acctaactat caagggtctt tgtgtggaaa aatcagtttt tattcgattt
     7081 cttctaccat ttcctgcaaa cgctgttcat attttttgtt tcctttatat aaatcaccaa
     7141 tcgtccatgc caacaggagg aggacaagaa caatgattac ataagcgatg tgatcagcca
     7201 atgctagttg agcggaagtg gcttgctcgc cgaagaaact ttcgatctgt cctggtaatc
     7261 ctcgtaagtt caagtaagtt agactaatca cagagaacca gccaagaagt tttacgatga
     7321 atgaattttt aaatcggcta cccatctccg cttggctgtt ggtcatcatc agtaaaggga
     7381 tcatcgaaaa cggcaacgca aaagccaaga acacctggga ttcattcatt aagttattca
     7441 aagcggtatg ttcttcgatc gttccttttg tacttgtaaa caaaacacaa atcaatacag
     7501 gaatcacaga aagcaatcgt gtgaccagtc ttcttagcca aatcgggata cgcatatgga
     7561 tgaatccttc catgatgact tgaccggtca acgtaccagt aatagtagag ttctggccag
     7621 aagcaagaag tgctacagca aataaagtgg acaagatacc agattttgct acagcaatca
     7681 agataccatt gctcatagct gatggatcag ataaagcttc atacagacca aaaaaggaag
     7741 gatcttgaac agaaccggtt ttgaagacag cgacacccat aatcaatagc agcgcattga
     7801 ctagaaaagc catcgacaat tggatattag agtcccaagt agaaaaacgt acagcttgtg
     7861 ctacatcttc ttcgtcttgg tgatcgatct tccgagtttg tgaaactgct gaatgtagat
     7921 ataagttatg tggcatgact gttgcgccaa taatccctaa tgctccagtt agcggagttt
     7981 cgccaccaat gacaggtgag tcggagaatg tatggctgtt agggataaag ccagcaaata
     8041 aactgtgcca atctgggtta gacaagccaa cttgatagac aaaaacgaat aaaatgacga
     8101 aaatcaagca gacaaccagt gcttcgatct tacgaaaccc gacttttgtc agtagtaaga
     8161 gaacgaaaac atctaataca gtaataaaga cggcaatcac caatggaata tcaaaaagta
     8221 agtagagggc gattgctgca ccaatgactt ctgcaatatc tgttgccata atggctaatt
     8281 cagtcaagat ccataaaatg acacccaatg tcttacttgt gcgtgctcgg attgcttggg
     8341 ccaaatccat ttgggtcacg attcctaatt ttgctgccat atactgtaaa agcatagcaa
     8401 tcagactaga catcaaaata actgacatca ataaatattg gaaattttgt cctccggtga
     8461 tcgaagtaga ccagttaccc gggtccatat acccaactgc tactaacgca ccaggtccag
     8521 agtaagcaag aagtgtttta aaaaaacttt tcccctttgg tacatccact gttccattta
     8581 cttcagctaa agataaacca tttgtatgtt caataagtcg atgcttatga aattgtttgt
     8641 tggattgatt attcaagaaa aaaccacctt tcctatttat ctacgattac attctacacc
     8701 tcaaaaagaa aaaattaaag aaaaagattc ggtatgccta attttttttc gtggcgtgtc
     8761 ttgctagatg acgaaagaaa ttttgttctc ctctaaaaaa accactattt tttgcatgaa
     8821 actaaagaat acgcctttct tttttcaata aactttagta aaataagtca ttgagagcaa
     8881 ttcggaaata gaaaggagag atttatatgt tatcaacagg tattgtcgta cttattgcta
     8941 ttattgcttt gctaatcggc gcagtaggtg gctttttcct agcgcgtaaa tatatgaaag
     9001 actatttaga aaaaaatcct cccgttaatg aggacatgtt gcgatcaatg atgatgtcaa
     9061 tggggcaaaa accatctgag aagaaaattc gccagatgat gcagcaaatg aagaaccaag
     9121 ggaaaaaata ggttctgacc caacgacttt ttacgggctg attttttcag tccgtttttt
     9181 atttcgtaat ttgttagact attcagatta ttcagcaaaa agacaagcac agacaatcct
     9241 aggctcttga taaagacaaa ggaggagtaa aatgtcgatt tttagaaagt taggctggtt
     9301 ttttcgtcag gaaaagaaac attatttaat tggggttttc tcgctattcg cagtggcctt
     9361 ggtacagtta gttcctccaa aagtgattgg gatcatcatt gatgagattg ccgataaaaa
     9421 tttatcagta aagatcattt tcgggtggat cttcctattg attatcgctg cttttttaca
     9481 atatcttttt cgttatatct ggcggacgaa tatttgggga agtgcagctc ggttggagaa
     9541 agaacttcgg cagcagttat tcaaacattt tacagaaatg gacaatctct tttaccaaaa
     9601 atatcggact ggggatttga tggctcatgc aacgaatgat ttgaatgcga tccagaatgt
     9661 agcaggagct ggtatcttga cctttgcaga ttcagtgatt acgggcggaa caacgatcat
     9721 tgcgatgatt ttgtttattg attggcgctt aacactgatt gcgctgattc ctctgccttt
     9781 gctggccgtc acttcacgag tgctggggtc gaaactccat gatgcttttc gtgattcgca
     9841 agaagctttt tctagtatca atgataagac acaagaaagt atcactggga tcaaagtcat
     9901 taaaacattt ggacaagaaa aagaagatct agcggatttt gaagaaaaga ttgaagatgc
     9961 aattattaaa aacaaacgag tcaatttttt agactcctta tttgatccat tcattacctt
    10021 gattatcggt ctgtcttatg ttgcaacaat tattttaggt ggaaagtatg tcttggaagg
    10081 cacgatctcg atcgggcaga tggtctcatt tattagttat atcggtatgc ttgtttggcc
    10141 gatgtttgcg attggtcgat tgttcaacgt attggaaaga ggaaatgctt cttatgatcg
    10201 cgtcaatgaa cttttgcatg aaaaaacgca tatcgtggaa caactgggtg cagtgcagac
    10261 cccggcaaaa ggcgagctga atgtagctat ttcctctttt aattatccga aagaagaatc
    10321 accagcatta gaacaaatca agtttgcaat ccaagaagga gaaacattag gtattgttgg
    10381 gaaaactggt gcgggaaaaa cgacgatcat caaattactg atgcgtgaat acgatgatta
    10441 tcaagggaaa atccaatttg gcggacgttc gatcaaagaa tattcattga atgctttatt
    10501 aggatcaatc ggctatgtgc ctcaggatca cttcttgttt tcgatgacca ttcgagataa
    10561 tatccgtttt tctaatccaa aactttcaga agaagaagta gagcatgcgg cttctttagc
    10621 atttatcaac aatgaaatca aggaattacc aaaaggttat gataccttag ttggagaacg
    10681 tggagtctct ttatctggcg gacagaaaca aaggatttcg attgctcgag cattgatcaa
    10741 aaatcctgaa ttattaatat tagatgatgc actttctgca gtagatgcca aaacagaaga
    10801 agctatcttg cataatttaa aagaaataag aaaagagaaa acaactatca tttcagctca
    10861 ccgtttaagt agcgtgatgc atgcaaaaga aatcattgtt gtcgatcaag ggaaaatcat
    10921 tgaacgagga acccatcatg agctgcttgc actaggcggc tggtatgaac aaatgtggga
    10981 gaaacagcaa ttagaagcga agatagaagg aggcgaggcc tgatggatga aaattatcaa
    11041 tcggaatgga caaaatcgat tccacttaaa gaacagaaaa agatcatcaa acgactgatg
    11101 aagttcgcca aaccttttag aagaacgttc atcgttgcta tcttatttgc gtttgctctg
    11161 tctgtgatca atgtcctatt gccaaggatc atccaaactt ttatggatga tcatctggct
    11221 aaacagtcgg ctacgacaca agttattttg ttttttgctg gtatgtactt atttggagtc
    11281 atagtaaaaa gtatcatttg gtttttccaa tggtatttgt attccatggc ttcattgaag
    11341 acctatcagt atattagggt aaaattattt gaaaagctgc atacactagg tatgcgttat
    11401 tttgatcaga caccggcagg atcaaccgtt tctcgagtga cgaatgacac agaaacgcta
    11461 tttgaatttt ggtacgtctt tttgatggta ttgactggga tctttgcagt cgtttcttca
    11521 ttcattgcca tgtttcaaat caatggagaa attgccttgt ggactttggt gttcttgcca
    11581 attttaggaa tagtcatctg gtattaccag aaatttagtt ctcgtattta ccggaatatg
    11641 cgggaaaaat taagtcagct gaatacgaaa ttgaatgaat cgatttccgg catgcgtatt
    11701 atccagcagt ttcgccaaga agctcgttta gctaaagaat ttgaacaagt gaacaatgag
    11761 tatttagaca cgcgttatgc gatgatcaaa acaaattcga ttctgttaag tccgatcatc
    11821 aattttctat atgcattggc gattgcgttg gtgttgactt tgtttgggat cgatgcgttg
    11881 cattctcccg tagaagttgg actgatctat gcatttacga cctatgtcca gggatttttt
    11941 aatccaatgt ctcaaatgat ggactttttg agcatcttca cagatgggat ggttgctggt
    12001 agtcgtatct tgaagattat ggatacagaa gaatatgcac cacaacaaaa ccctggagca
    12061 gatcaaatga ttacttcagg taagattgag tttcgcaatg taagcttttc ttatgatgga
    12121 gagcacaatg tattaaacaa tatctctttt gttgctaatc cgggagagac cgttgcatta
    12181 gtgggacaca ccggaagtgg gaaaagctcg atcatcaatg tgttgatgcg gttttacgaa
    12241 ttttatgaag gtcagatatt gattgatgat catgacattc gagaatatcc aatggaagag
    12301 ttacgtaaga aaatggggct ggtcttgcaa gatgctttct tgttttatgg ggatatcgca
    12361 ggaaatattc ggatgatgaa tccagctatc acagacgaac agatacgcca tgcggctgag
    12421 tttgttcaag caaacaaatt catcgatgaa ttaccaaaag gatatcactc aaaagtgatc
    12481 gaacatggtg ccagctattc tagtggacaa cgacagctga tcacttttgc gcgaacaatc
    12541 gtgactgatc ctaaaattct agtattggat gaagcaacag caaatatcga tactgaaaca
    12601 gaaggattga tccaagaagg attggcgaaa atgcgacaag gacggacaac gattgcgatt
    12661 gcccatcggc tgtcaacgat tcgagatgca gaattgattt tagttttaga caaaggtcat
    12721 atagtagaac gcgggaatca tgaaacactt ctagcgcaaa atggtttgta tgcagatatg
    12781 tacaaactgc aaagcagtgg tatataaaag atgttgtgac agtagcattt tgacctaagc
    12841 actagagtga aaataaaaca tagcttccca tatttttcat ctgtgagagt tagtgtcgta
    12901 gttaccagat ttaaaaaaga cgacgtcagc tagtcagaag tgcctaatag cccctaagaa
    12961 agcgttgaca aaatcgtttg ctttcgataa tataaagaaa acgatacaac ttggatagtg
    13021 attgcctgat gcaagctaaa cctaatgagt ctcccatggt ttcatggacg gctttttggg
    13081 tttttctttt tgttttggat acagccctat atatcatgga aaggagcaaa acatgctgat
    13141 tgagaaagta ctgaataaca acgtcgtaat aacaagggat gaaagagatc gggaaatgat
    13201 tgtgatggga aaaggattgg cattcaaaaa gaaggtgggt gacccgattc ttgattcatg
    13261 catcgacaaa aaatttcagt tgacaaatga cggactttca caacagttcc aagaattggc
    13321 acaagcgatt cctcttgagt acttagaaat ttcttgtgaa atcattgaat atacaaaaga
    13381 ggctctgcaa gtccatttaa atgattctgt gtatatttca ttgacggatc acttgtatac
    13441 agctatcgaa cgtcaaaaaa tggggatctt tgtgccaaat gtattgctgt atgatatcaa
    13501 acaactgttt cctaaagagt atgccattgg gaaaaaaaca gtgaagaaaa tctcggataa
    13561 ataccacatc aagttatcag aagacgaagc cggatttatc gcattgcatt tagtcaatgc
    13621 acaaggcatt caaagccagc aagacaatac ctacctgttt accacaacaa tacaggatat
    13681 tttaaatatc gtccgctatt actataagat tacatttgat gaagattcag tccacttttt
    13741 tcgattcgtc acacacctgc gtttttttct gacacgtgtt ctagatggag cgaaatcaga
    13801 agatggagaa gtttctgagg aattgttcca agttgttcaa aaaaaatata aggatacgtt
    13861 tggttgcgtt gagaaaatag cagacttttt aaccggggct ttcgactacc agatgtccac
    13921 ggatgaaaaa atgtatttaa cgatccatac tgcgcgttta gtgcatgaga atcgttgtaa
    13981 ataaataaaa aaggaggaca aattaacaag aacgtatgaa aataagggta gtgacattca
    14041 gttggctaaa ccttccacta attaattgga gggaatagaa atgggaaaat accaggaatt
    14101 agcagaaaac atcgtaaaga atatcggagg aaaagaaaat atccgtagct tgacacactg
    14161 tgtgacgcga cttcgtttca aactaaagga ggaaaataaa gcaaaggatg atgttttgaa
    14221 aaagatggaa gggatagtca ccatcatgaa aagcggcggg caatatcaag ttgtaatcgg
    14281 gaatcatgtg ccagatgtct atgcagaagt actgacagta gccgggattt ccgatgaagt
    14341 agaagaagag gtttcttcaa gtagtaatct gttcaatcga ttaatcgaca tattaagtgg
    14401 ctgtttccag ccttttttag gtgctttagc tgcagcggga atggtcaaag gattaaatgc
    14461 tttgttcttg tttctcaaat tttatactga aggatcgggt acgtacatca tgctgaatgg
    14521 gataggggat gcaatttttt atttcatgcc agtgatctta gggtacacag cagcgaaaaa
    14581 atttcatctg aatccgatgg tggggctcgt gattggtgca gcactttgtt atccgactgt
    14641 acaaggagca gcactacaaa gtggtttccc tgatggcagt cctgcgcctt atagcttgct
    14701 gggtcttccg gcttatgaaa catttttggg tatcccatgg gtgggcagca gttataccag
    14761 tagcgtcgta cctgttatct tcattatcgc atttgccgga caagtccaaa aaattgccaa
    14821 gaaatggatt cctgcagtcg tccagacctt tttggttcct ttttctgtgt tattaatcgc
    14881 acttcctgct ggctttttga ttatcggtcc gattgtcagt atactgacag atctattaag
    14941 cacgggattc caagcattga tggacttttc tccagctgtg tatggtgcag ttctagggtt
    15001 tctttggcaa gtactggtga ttttcggact tcattggagt attatccctc ttgcgattat
    15061 gcagctagcg caaaatgggt ggagtcagat gttagtaggc gtttatggcg cgagttttgc
    15121 acagacagca gtggtattgg cgatgtattt caagttcaag gatcaaaaaa tgaaggcgct
    15181 ttgcatgcca gctgtgattt caggaatctt cggcgtaaca gaaccggcta tttatggaat
    15241 caccttaccg aagaagaagc catttattta ctcgatgatt ggtggtgcca tttcaggaat
    15301 ctatttgatg gcatctggaa caatcagcta cacaatgggc ggattaggga tttttggggt
    15361 ggtaaactac atcacggaaa cagacgccag tggtatgttt caagcgttta tcggcattgc
    15421 tattgcttcg gtaatcgggt tttcgctgac ttttttcttt tggaaggatg aagcaggtga
    15481 aacagatgct atacagaaaa aatcaggtaa agaaattgta aaaacaccga tacaaggaca
    15541 aatcgctcca ctaaatgcag cgaaggatgc agcttttgca caaggaacac taggtcgagg
    15601 aatcttgatc taccctgaaa aaggagaagt acgtgcccct tttgacggaa cagtcatgac
    15661 cctgttcccg acgaaacatg caattggaat ggtttctgaa acagggctgg aattgctgat
    15721 tcatgtcgga ttggatactg tgcagttgga agggaaatat tttgaaagct tagtgcagca
    15781 aggagaaaga gtgaaacaag gcgatctgtt gctacgcttc gacaaaaaga aaatcgaaaa
    15841 ggaaggatac agtttagaaa cacctgtgat cgtcaccaat tacacggatt atttggatat
    15901 tttggaaaac caaggggaga cggtcgggcc agacgatgca cttatcaccg cactgacctg
    15961 aacggttagg tgaagaatgg aataggctta actattcttt ttattccact tctttatttt
    16021 caaaattgaa aggctctttc atgatggctg agctgatagg gcgtttatcg aatttttgac
    16081 cgacagaaat tagatggact aaattctgtt gataattttc tgcaaaaaca caactatata
    16141 acacgtctag aagataacaa attgatgtat tgatcgtaaa attaccaatt tttgaataca
    16201 gtcgctcgcg tgtcgtaagt cgcaaagcgg ctgtactcat tgcagatagc gttgaatcac
    16261 cgatactggt caaagctaaa attggaacat gattttgttt caatatttct gcggtacgaa
    16321 gaatcacttt attttcaccc gtataagaaa tcaggatccc gcacatatcg ctcgtgcaat
    16381 tgtacgcttc atagaatgat tcaccaaaag tggtgacaat tgtgacatcc attttgatgc
    16441 gcttcatttt cagcatgaaa tcttgagaaa tcaaagtatt cgagttactg gcgaagatca
    16501 cgattttttt tgcctggagc agtaaatact tggctttttg cagttggtca tgatgaatga
    16561 gagaaagtgt atcttgaatc gtcatctgtt ctaaagaagc cattttattt gcgatcgtca
    16621 taataccgtc tgtggtggaa aaaggaagat tcgcatctac gtcttgaaaa ttcccttcaa
    16681 gataggctgt ttcagctaaa aaagcattac gaaaactgac ccagccatca aaacccattt
    16741 tttttgctac acggatgagt gtagacgggt ggacatagca tcgctcagcg atctcttgga
    16801 tggtcatttc gccaagtgct ggagaatcgc ttaagagaaa ctggatcact tcattttctg
    16861 aaggagaaaa ggagatttct ttgatttttt cacgtaataa catgtagtta ccttcttctt
    16921 atttttttac gtaaattata acttttttga aaatgttttg taaacggatt tatactatat
    16981 aacttaaaat agactttttt tgacaagata tttacaaaaa tccac
//
LOCUS       DAKBVG010000039        16957 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000039 DAKBVG010000000
VERSION     DAKBVG010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16957
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.039"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(<1..1003)
                     /locus_tag="OUD09_000939"
     CDS             complement(<1..1003)
                     /locus_tag="OUD09_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCT9692224.1"
                     /translation="MLLNFKMTLTMLAFSLLVTGVILVSFFFLRNNLEGISIASTLGE
                     DVYFMEEGKTVQAETQMEASKSSNNQFMTISMIDKAYQDELFKYLPAMVLVICSAVML
                     LTFILWLVLRKIYSNQILAITRDLTFIDQVPKNTVKDEALNAAFNKLKDKFDGHLEDY
                     RRLNSYLTHEQKNAIAILRTNLEIERQHADQGNLMLLDRMTDSIDDILTLSNNSEDLS
                     TEVVDVSLICAEVCDAYKMNYPNLLFAFTEDQPTLIHGRDRWIYRAVSNLVDNAIKYG
                     EGKLVIVTISNQKGSVIIEVKDQGIGIPQEKQASIFSHQYRVHGLKQDGLPKTMDFRD
                     "
     gene            complement(994..1662)
                     /locus_tag="OUD09_000940"
     CDS             complement(994..1662)
                     /locus_tag="OUD09_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCT9692225.1"
                     /translation="MRVLVVEDNRELVLSVKKGLEQENISVDVAYDGESGEEKAFINS
                     YDGILLDLNLPKKDGLSVLQSLRAEEVESPVIIITARDEIEERAKGLDFGADDYLVKP
                     FELLELKARIRAVIRRYHGRALPQISIGALKIDPLGRRVSYQKTEVVLKPKEFDILLC
                     IAQRHPAVVSSEEISEHVYDEDFDPFSSVLRVHLARLKKKLAEAAEKEVLKTIRAKGY
                     QLCY"
     gene            complement(2118..3104)
                     /gene="lacD"
                     /locus_tag="OUD09_000941"
     CDS             complement(2118..3104)
                     /gene="lacD"
                     /locus_tag="OUD09_000941"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HCT9692226.1"
                     /translation="MNEEKRQYIKQLCNEDGVIGALAIDQRGALKKMITKFKGSEAEE
                     SEIVEFKEIVSSQLTKYASSILLDPEYGLPAADVRAKNAGLLLAYEKTGYDASTPGRL
                     PDLLSIWSVKRLKEAGADACKFLLYYDVDEAEEINNQKHAFIERIGSECQAENLPFFL
                     ELVSYDANISDATSKEYAIKKPHKVNDMMKEFSDPRYGVDVLKVEVPVNMNYVEGYGT
                     DEVVYTKAQAANFFKEQAEATNLPFIFLSAGVKAELFQETLRFAKNAGSTFNGVLCGR
                     ATWADGVESYIVDGKEAAMEWMNTQGRKNIEELNAVLKETATPVEVLTSSLS"
     gene            complement(3117..4043)
                     /gene="lacC"
                     /locus_tag="OUD09_000942"
     CDS             complement(3117..4043)
                     /gene="lacC"
                     /locus_tag="OUD09_000942"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HCT9692227.1"
                     /translation="MILTITMNPSIDISYPLETFKLDTVNRVRNVRKTAGGKGLNVTR
                     ILKQLDTEVIASGLIGGFLGEEIKKDLTQNEIAHDFLPISGETRNCIAVLHDGKQTEI
                     LENGPVITEQEARAFLQHFEKLAVKAEIISFSGSLPEGLPADFYRQMLQVCQPKPVVL
                     DCSGTALREVLRGSVKPKLIKPNTEELAGLLGREISTELSDLKAALSDSMFDEIEWIV
                     VSMGGDGAFAKHHETFYCVTIPKIDVVNPVGSGDATVAGLTAAIQQGLSDEAVLKQGN
                     VLGMLNAQEATTGYVQLANYQKLYDQILVEKV"
     gene            complement(4056..4571)
                     /gene="lacB"
                     /locus_tag="OUD09_000943"
     CDS             complement(4056..4571)
                     /gene="lacB"
                     /locus_tag="OUD09_000943"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HCT9692228.1"
                     /translation="MKIAIGCDHIVTDTKMAVSDFLKSKGHEVLDMGTYDFTRTHYPI
                     YGKKVGEAVTSGEADLGVCLCGTGVGINNAVNKVPGIRSALVRDMTTAIYAKEQLNAN
                     VIGFGARITGEFLICDILEAFINADYQETEENKKLIAKIDHVEKASDKQSDPHFFDEF
                     IEKWDRGEYHD"
     gene            complement(4590..5018)
                     /gene="lacA"
                     /locus_tag="OUD09_000944"
     CDS             complement(4590..5018)
                     /gene="lacA"
                     /locus_tag="OUD09_000944"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HCT9692229.1"
                     /translation="MRVVIGSDSDGMELKELIKKKLIKEGITVVDKSETASEDFVEST
                     LAIANDLNENEGSLGIAVDGYGAGSFITATKVKGMVAAEVSDERSAYMTRKHNNSRMI
                     TLGSKIVGDELALNIVKEFLQANYDGGRHQVRVDMLNKMA"
     gene            complement(5038..5811)
                     /locus_tag="OUD09_000945"
     CDS             complement(5038..5811)
                     /locus_tag="OUD09_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCT9692230.1"
                     /translation="MLKTERQRSILAMCDRYGTITVKFIQEVLNVSDMTIRRDLEELA
                     QKKKLLRVHGGAQKIDYLEMSTSIEANELSHTEKKKLHTKEKEYIAKNAAAHINQEDD
                     TIFLGSGTTIELMTRYIKPSSLRIVTNSLPVFNLLSEIKRFELYLIGGLYREKTGAFV
                     GSIANEAVQKIGIKKAFVGVNGLKGSEVSNFSIEEGTLQKIVLDNAAEKCLVSDANKF
                     DHSDFYNFYDLRKIDKLFTDSTLSAELKSYYQQFTKIIQ"
     gene            5976..6455
                     /locus_tag="OUD09_000946"
     CDS             5976..6455
                     /locus_tag="OUD09_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCT9692231.1"
                     /translation="MNEFFFKDTVYVSDKQSQEEVFEEVYQDLLKKELVTEDFLENLL
                     ERERNYPTGLDLSPVSKILPDIAIPHTESEFVRTTRIIPIKVKNEIPFYNMISPSDQI
                     SASFLFMILNENGEAQAGMLANIMDFINSVDEEKLFSFFKLEDTEAIYHFLEEHFYI"
     gene            6512..6814
                     /locus_tag="OUD09_000947"
     CDS             6512..6814
                     /locus_tag="OUD09_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003063077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HCT9692232.1"
                     /translation="MIKILAACGAGVNSSHQIKSALETELKNRGYQVTCDAVMIKDIN
                     EEMLSHYDIFAQIANTDLGFDINIPVVDAGAILYRIPAMAEPVYQSMESAIQSLNK"
     gene            6836..8293
                     /locus_tag="OUD09_000948"
     CDS             6836..8293
                     /locus_tag="OUD09_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCT9692233.1"
                     /translation="MNTIIDIANKVFTPLINLGAAPLMTIVLTVIALFFKVKPSRALE
                     GGLKLGIALTGIGAIIGILTTAFSDAMTAFVERTGISLNITDVGWAPLATITWGSPYT
                     LYFLLIMVIVNIVMLAMKKTNTLDVDIFDIWHLAIVGLFAIFCGANLFIASALVIFIG
                     VLKIWNSDLMKPTFNDLLNAPDSNPMTTTHMNYMMNPIIMVFDKIFDKIFPWLDKYDF
                     DAAKLNSKIGFWGSKFAIGIYLGIFVGLLAGQTPTQIFSLAFTAAVCLELFSLIGAWF
                     IAAVEPLSQGITDFANKRLKGRTINIGLDWPFIAGRAEIWAAANVLAPIMLLEAIILP
                     GNKLLPLGGIIAMGVTPALLVVTRGKLIRMIVIGAIELPLFLWSGTLIAPFVTQTAKA
                     VGAFPAGLNGSAMISHTTMEGPIEKFLGYLVGNASQGQIEFVIYAALALGAYLLIFIW
                     YARQMKKRNAAYAAEKEENASPATAHNMTYAAEAK"
     gene            complement(8886..9638)
                     /locus_tag="OUD09_000949"
     CDS             complement(8886..9638)
                     /locus_tag="OUD09_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCT9692234.1"
                     /translation="MLKTDRFQAIMSLLEVNGTVRVSDIMEKFSVSDMTVRRDLDEME
                     KQGLLERVHGGAKLKDFYRHVELSHREKQIMLQEEKQRIVEKANEMINDGDTIFLGPG
                     TTIELMSKIIQGDSLRIVTNCLPVFEELNKRKGNRKIYLLGGEMRETTQCFVGEITNK
                     SLQDMNFHKAFFSCNALNEQKIMTSTFEEGQTQQIALNNSVERFLLIDHSKIGKRDFS
                     VYYNLNDVTVVITDDDEQKHYEKIEKEEKYVV"
     gene            complement(9853..10089)
                     /locus_tag="OUD09_000950"
                     /pseudo
     CDS             complement(9853..10089)
                     /locus_tag="OUD09_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
     gene            complement(10099..11057)
                     /locus_tag="OUD09_000951"
                     /pseudo
     CDS             complement(10099..11057)
                     /locus_tag="OUD09_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(11263..12945)
                     /locus_tag="OUD09_000952"
     CDS             complement(11263..12945)
                     /locus_tag="OUD09_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HCT9692235.1"
                     /translation="MNTIIKQIEKGKPFFEKISRNIYLGAIRDGFLTAMPAILFSSIF
                     IMIAAVPEVFNVTLPEAFSTWLWKVYGYSMGLVGLLVVATTARCLANSMNRQLSDSGK
                     QINEVSVMLASICGFVMMAADQIDGGIATDYLGTKGLLAAFVSAFVTVNVYKFCVKRD
                     LTIRMPKEVPGTITQMFRDIIPFSIAVFIAVLIDTGVRQFFGTSFAAGVIQLLQPLFS
                     AADGYVGIAIIWGAMAMFWFVGVHGPSIVEPAIAAIIYANVETNFELFKNGAQASKVL
                     TVGLGNFVGTMGGTGATLVVPFLFMLFAKSKQLKAVGKASFIPVSFAVNEPLLFATPI
                     ILNPYFLIPFLLAPIANVWIFKLFVDVLGMNSFISVLPWATPAPIGLVLGTGFSVLAI
                     VLAVVLIVVDAIIYLPFVKAYDSELVEQELKNIAAEEVKEELVEEIQEVGATAAVIVD
                     EAKASVGEGKRVLVLCAGAGTSAMLANALEEGAKETGVNLSAKAGAYGSHYDMMKNFD
                     LIVLAPQVNSYLDDLRKDADKLGIKVIATKGAEYINLTRDPKGAVDFVLKQF"
     gene            complement(12956..13279)
                     /locus_tag="OUD09_000953"
     CDS             complement(12956..13279)
                     /locus_tag="OUD09_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCT9692236.1"
                     /translation="MDRKELQLLGFEIVAYAGDARSSLLTVLKEVREGNFDNVERLLA
                     DADENLTLAHNSQTKCLAEEASGNEMEVGVIFIHGQDHLMTTILLRELINDFVELYKE
                     KFAKA"
     gene            complement(13302..14708)
                     /gene="lacG"
                     /locus_tag="OUD09_000954"
     CDS             complement(13302..14708)
                     /gene="lacG"
                     /locus_tag="OUD09_000954"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HCT9692237.1"
                     /translation="MNYKTLPEDFIMGGATAAYQAEGSTKEGGKGQVAWDTFLVEKGR
                     YTADPASDFYNQYPVDAELCKKFKIDSIRMSIAWSRIFPKGYGEVNQTGVEYYHKVFA
                     ELNKHGIIPFVTLHHFDTPKELFDNGDFLNRENVEHFVNYAQFCFDEFSEVHYWSTFN
                     EIYPVASNQYLIGSFPPEVQFDIEKTVTCLHNMMYAHAKTVNIFKDGGYKGEIGVVHS
                     LETKYPASDKEEDKHAAKLDDALSIKFLLDATYLGYYSDETMAHINEILAANGQSADI
                     DSNDFREMKKAANRNDFLGINNYGSHWVKEYKGESTLHYNTTGEKGSQVYCIKGIGER
                     IVREDLPKTDWDWIIYPKGLYDLLVRIQDEYPNYNKIYITENGLGYKDNFEDGIILDD
                     DRIDYLRQHFNAISDAIKMGVNVKGYFIWSLMDVFSWVNGYTKRYGLFYVDFDTQKRY
                     PKESAYWYKFVSETKTII"
     gene            15175..16182
                     /locus_tag="OUD09_000955"
     CDS             15175..16182
                     /locus_tag="OUD09_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCT9692238.1"
                     /translation="MVTIRDIAKKSGYSTATISRLFKGDESLSITSDTKNKIIVTAKS
                     LGYDLTKIKTTLYKIAILFWISEEQLKKDVYYQHVKDDLKNYSKKANLDLIFISKNDG
                     VKSIPDDITGFIAIGQLTKKELYYLNQKGYKGVIRGINPLPEHFDTVEPDFHEMARQA
                     INYFINEGFTKIGFIGGNFFNLETGKEEIDSREYAFRYHLEKSGLLNEKYIFSQGSFT
                     VDEGYQLTLKMKAELNEDMPEACFIPSDTIAAGVLQGCHELGINIPKDMAVISINDDD
                     IAKFVAPPLTTFKIDIEEIAKTCVDLLSDQLVYSRDISKKTLIGAKLTVRKSFIPKEE
                     V"
     gene            complement(16576..>16957)
                     /locus_tag="OUD09_000956"
     CDS             complement(16576..>16957)
                     /locus_tag="OUD09_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-dehydro-3-deoxyphosphogluconate aldolase"
                     /protein_id="HCT9692239.1"
                     /translation="NSLRYLEFREGAFDFQTSQQNLLTIPGAMSPTEIFKMFEMGADI
                     VKVFPANCLGPEYFNQVQAPLGSLPLMAVGGVDQTNAQNYLNNGASYVGIGSKFFEKS
                     AVHQLNYERLMELAESFIDSLRVE"
BASE COUNT         5079 a         3609 c         2787 g         5482 t
ORIGIN      
        1 tgtccctaaa gtccattgtt ttaggtaagc catcctgctt gagcccatgc actcggtact
       61 gatgagaaaa aattgatgcc tgtttctcct gaggaatgcc aattccctga tcttttactt
      121 caataatcac gctacccttt tggtttgaaa tggtgacaat taccagtttt ccttcaccat
      181 atttgattgc attgtcaact aaattcgaaa ccgcccgata aatccaacga tctcgtccat
      241 gaatcagggt tggttgatct tcagtaaacg caaacaaaag attcggataa ttcattttgt
      301 aggcatcaca aacttccgca cagatcagtg aaacgtctac gacttccgtg ctaagatcct
      361 ctgagttatt ggataatgtc aaaatatcgt caatactgtc ggtcatccga tctaaaagca
      421 ttaaatttcc ttgatcggca tgttgccgtt ctatttccag attcgtacgc aaaatagcga
      481 tggcattttt ctgctcgtgg gtcagataac tatttaatcg tcgataatcc tccaaatgac
      541 catcgaactt atcctttagc ttattaaaag ccgcatttaa tgcctcgtcc ttcacagtat
      601 ttttaggaac ttgatcaata aacgttaaat cacgcgtaat cgctaagatt tgattactgt
      661 aaattttgcg taaaaccaac caaagaataa acgttaataa catgacggca gaacaaataa
      721 ccagcaccat cgctggtaaa tatttaaata gttcgtcctg gtaagcttta tctatcatcg
      781 aaatagtcat aaattgattg ttggaagatt tcgaagcttc catctgcgtc tctgcttgaa
      841 ccgtcttacc ctcttccata aaataaacat cttcacctaa ggtagatgcg atactgattc
      901 cctccaaatt atttctcaga aagaagaaac tcactaaaat cacgcctgtc accaacagtg
      961 aaaaagccag catcgttaac gtcattttaa aatttaatag catagctggt acccctttgc
     1021 tcgaatggtt tttaagacct ctttttcagc tgcttcagcc agtttctttt ttagacgagc
     1081 cagatgcact cgcagaaccg acgaaaaagg atcaaaatct tcatcgtaga catgttcaga
     1141 aatttcctca gacgaaacaa ccgctggatg acgctgagcg atgcacagta agatatcaaa
     1201 ttccttgggt ttcaggacca cctctgtctt ttgatagctg acccttcgac ctaatggatc
     1261 aatcttcaaa gcaccaattg aaatctgcgg cagcgcacgt ccatgataac gacgaataac
     1321 cgcgcgtatt cgtgctttta attctagtaa ctcaaagggt ttgactaaat aatcatctgc
     1381 tccaaaatcc aagccttttg ctcgttcttc aatctcatca cgtgcagtaa taatgataac
     1441 tggactttcg acttcttcag cacggagact ttgtaaaaca gataatccat cttttttagg
     1501 cagatttaaa tctagtagta tcccatcgta tgaattgatg aaggccttct cttctcctga
     1561 ctcgccatca taagctacat ctacagaaat attctcctgc tctaggccct tttttacgga
     1621 taaaaccagc tcacgattgt cctcaaccac cagtacacgc atgtatattc actcctcata
     1681 cggatttagt aattacactt atgcttttta gcatagtaca cgctcttctt caaagtccac
     1741 gttctttttg tgaagatttg aagaaattca tacattctgt taaaaaacac ataattaaga
     1801 atgattttac tgtttgaaaa tacctagaat agaaaaagac tggaaccttt cctccagtct
     1861 cactactttc tatttgcttg gttttcttgt atatactttt caaagttcgt tggtttatga
     1921 ccaataagcc tcgttagaat actgaaaatt gccatcaagt cattaagtac cacatgtaag
     1981 aaaatctctc taaagaaaaa ccccttcgtt ttaactcact tactatttta gaattcgttc
     2041 ccctaataat gcagcatgat tttacgcttt atgtataaaa aaagactgca agcaattttg
     2101 cttcacagtc tgatttctta tgatagtgag cttgttaaca cttccaccgg tgtagcagtt
     2161 tctttcaata cggcatttaa ttcttcaata tttttacgcc cttgcgtatt catccactcc
     2221 atcgctgctt cttttccatc aacgatatag ctttctacac catctgccca agtcgcacgg
     2281 ccgcaaagta caccattgaa ggttgagccg gcatttttcg cgaagcgcag tgtttcttgg
     2341 aacaactctg cttttacacc ggcgcttagg aaaataaatg gcaggttagt ggcttcagcc
     2401 tgttccttga agaagttagc cgcttgtgcc tttgtgtaca cgacttcgtc tgtcccatag
     2461 ccttccacat aattcatatt gacgggtact tccaccttta acacatctac gccataacgt
     2521 ggatcagaaa attccttcat catatcgttg actttatgag gtttcttaat ggcgtattct
     2581 ttcgaagtcg catcagagat gttcgcgtca taagaaacaa gctccaagaa gaagggtaag
     2641 ttttcagcct gacattctga gccgattcgt tcaataaagg catgtttttg attattgatt
     2701 tcttctgctt catcgacatc atagtaaagt aggaacttac aagcatcggc gcctgcttct
     2761 tttaaacgtt taactgacca gatcgataat aaatctggta aacggccggg agttgacgca
     2821 tcgtaaccag ttttttcata agctaacagc aagccggcat tttttgctct aacgtcagct
     2881 gctggcaagc catattcggg atctaataaa atcgatgagg catatttcgt taattgtgaa
     2941 gaaacaattt ctttaaattc aacgatttct gattcttctg cttcgctgcc cttgaatttt
     3001 gtgatcatct tcttcaaagc cccgcgttga tcaattgcta aggccccaat cactccatct
     3061 tcattgcata gttgcttgat atattgacgt ttttcttcgt tcatttccgt atcctcctac
     3121 actttctcca ctaagatttg atcatatagt ttttgataat tggctaattg gacatagcca
     3181 gtcgtcgctt cctgagcatt cagcattcct aaaacattac cctgttttaa cacggcttca
     3241 tccgaaagac cttgttgaat cgcagcggtc aatccggcaa cggtcgcatc accagagcca
     3301 actggattga cgacatcaat ctttggaatt gtcacgcaat aaaaagtctc gtgatgtttg
     3361 gcaaaggctc cgtcgccccc cattgaaacc actatccatt caatttcatc aaacatcgaa
     3421 tctgacaagg cggcttttaa atctgataat tctgttgaga tttctcgtcc tagcagacca
     3481 gccagctctt cggtattggg ttttattagc ttaggcttta cactgcctcg caacacttcc
     3541 cttaatgctg tacctgaaca atctaaaacg acaggctttg gctgacaaac ttgcagcatt
     3601 tgtcgataaa aatcagcagg caatccttct ggcaagcttc ctgaaaaaga aatgatttcg
     3661 gctttaacag ctaatttttc gaaatgttgc aagaacgccc ttgcttcttg ttcagtaata
     3721 acaggtccgt tttctaggat ttccgtctgt tttccgtcat gtaaaacagc gatgcaatta
     3781 cgtgtctctc cgctgatagg taaaaagtca tgagcaatct cattttgagt caggtccttt
     3841 ttaatctctt cacctaaaaa accgccgatt aaaccactag caatcacctc cgtatctaat
     3901 tgcttcaaga tacgtgtcac atttaggccc ttaccgccag ctgttttgcg aacatttctt
     3961 actcgattga ctgtgtcgag cttgaaggtt tctaatggat aagaaatatc aatcgatgga
     4021 ttcatcgtta ttgttaaaat catttgtcca cccgcttaat cgtggtattc gccacgatcc
     4081 catttctcga taaattcatc gaagaaatgc ggatcagact gcttgtcact ggctttttcg
     4141 acgtgatcga ttttggcgat caattttttg ttttcttcag tttcttgata atcggcattg
     4201 atgaaggcct ctaatatgtc acagatcaaa aattctcccg taatccgagc gccaaagccg
     4261 atcacattgg catttagttg ttccttcgca tagatagcag tcgtcatatc gcgaaccagc
     4321 gcactgcgga taccaggtac tttattgaca gcgttgttaa tcccaacacc tgttccacac
     4381 aaacaaactc ctagatctgc ttctccgcta gtcacggcct ctccgacttt ttttccgtag
     4441 atcggataat gggttctggt aaagtcatag gtccccatat ccaacacttc atgtcctttt
     4501 gattttaaga aatctgatac tgccattttt gtatctgtga cgatgtggtc acagccaatt
     4561 gcgattttca tttcattcat ccttcctaat tacgccattt tatttaacat gtcgacacgt
     4621 acttgatgac gaccaccatc ataatttgct tgtagaaatt ccttaacaat attcaacgcc
     4681 aactcatcgc caacaatttt tgatccaagt gtgatcatcc gagaattatt atgtttacgg
     4741 gtcatatacg cactgcgctc atccgaaact tctgctgcta ccattccttt aacctttgtt
     4801 gccgtgataa agctgccagc gccgtagcca tcaaccgcga ttcctaaact accttcgttt
     4861 tcattcagat catttgcgat cgccaaagtt gattccacaa aatcttctga agccgtttca
     4921 ctcttatcta ctactgtaat accttctttg atcaattttt tcttgattaa ttcctttaat
     4981 tccatgccgt cactgtctga accaattact acacgcattt ttgtttcctc ctatttttta
     5041 ctgaatgatt ttagtgaatt gctgataata actcttcagc tcggctgaaa gagtagaatc
     5101 cgtaaataat ttgtcgatct tgcgcaaatc atagaaatta taaaaatcgc tatggtcgaa
     5161 tttgtttgca tctgaaacca aacatttttc tgccgcatta tctaaaacaa ttttttgcaa
     5221 ggtaccttct tcaatactga aattcgaaac ctccgaaccc ttcaatccat tcactccgac
     5281 aaaagccttc ttaataccga ttttttgaac agcttcattg gcaattgaac cgacaaaggc
     5341 tccagtcttt tcccgatata gcccaccgat caaatacagt tcaaatcttt ttatttcact
     5401 taatagattg aaaaccggca gactattggt aacgattcgg agagatgacg gcttgatgta
     5461 tcgtgtcatt aattcgatcg ttgtacccga tccaagaaaa atcgtgtcgt cttcttgatt
     5521 gatatgagct gccgcatttt tagcgatgta ttccttctct tttgtatgca gcttcttttt
     5581 ttcagtatga gaaagctcat ttgcttctat agaagtcgac atttccagat aatcgatttt
     5641 ttgtgcaccg ccatgaacgc gtaaaagctt tttcttctgc gcgagttcct ctagatctcg
     5701 gcggatcgtc atatctgaaa cattcagtac ttcctgaata aatttgactg ttatcgtccc
     5761 atagcgatca cacatcgcta agatcgaacg ctgtcgttct gttttcagca tattctgtac
     5821 ctccacgacc ttataataaa acatttacga acatttttca acacatattg ttcgtttatt
     5881 ttcgtttatg cgttgacaat gattttgtaa gcggttatac tcaacttgta aacatataaa
     5941 taaacataaa cgaactaata aaggagctgt tttgtgtgaa tgaattcttt ttcaaagaca
     6001 ctgtctatgt ttctgacaag cagtcgcagg aagaagtgtt cgaggaagtc tatcaagatc
     6061 tgttaaagaa agaattggta accgaagatt ttttggagaa ccttttagaa cgggagcgta
     6121 attatccgac gggattagat ctctcacctg tctctaaaat attgccagat atcgctatcc
     6181 ctcataccga aagtgagttt gtgcgaacca cccgtatcat tccaatcaaa gtaaaaaatg
     6241 aaatcccttt ctacaacatg atctcgccaa gcgatcagat ttccgcttca tttcttttca
     6301 tgatcttgaa cgaaaatggt gaagcacagg ctggcatgtt ggctaatatc atggatttca
     6361 ttaattctgt tgacgaagaa aaactgtttt cgtttttcaa attagaggac acagaagcga
     6421 tttatcattt tttagaagaa catttttata tatagaagtc tctgcctaac cgcaggataa
     6481 aaaataaact tattttaagg ggagaattat tatgatcaaa attttagcag catgtggagc
     6541 aggcgttaat tcaagccatc aaatcaaaag cgctttggaa actgaattga aaaatcgtgg
     6601 ctatcaagtc acctgtgacg ccgttatgat caaagatatc aatgaagaaa tgctgtctca
     6661 ttatgacatt ttcgcacaaa ttgccaacac cgacttgggt tttgacatta acatccctgt
     6721 cgttgatgca ggtgctattc tttatcgtat tcctgctatg gctgagcctg tttaccaaag
     6781 tatggaaagc gcaatccaat cattaaacaa ataggactaa agaaaggaaa agcatatgaa
     6841 tacaattatt gatattgcca ataaagtatt taccccccta attaatttag gtgcggcacc
     6901 gttaatgacc atcgtattaa ctgttattgc tttatttttt aaagtaaaac cgtcacgcgc
     6961 tcttgaaggc gggctcaaat tagggatcgc cctgactggg atcggagcta ttattggaat
     7021 tttaactact gctttttcag acgctatgac agcttttgtt gaacgcacag gtatttcatt
     7081 aaacattaca gatgtcggtt gggccccact agcaaccatc acatgggggt cgccatacac
     7141 gctttatttt ttactaatta tggtgatcgt caatattgtc atgttagcaa tgaaaaaaac
     7201 caacacatta gatgtggata ttttcgatat ttggcattta gcaattgttg gtttgttcgc
     7261 gatcttctgc ggtgctaatc tgtttattgc ttctgcatta gttattttca ttggtgtatt
     7321 aaaaatttgg aactccgact taatgaaacc aaccttcaat gacttgttaa atgcgccaga
     7381 cagcaacccg atgaccacga ctcatatgaa ctacatgatg aatccgatta ttatggtttt
     7441 cgataaaatt ttcgacaaaa tcttcccttg gttggataaa tatgatttcg atgctgccaa
     7501 attgaatagt aaaattggct tctggggatc aaaattcgcg attggaattt atttggggat
     7561 ttttgttgga ttactagcag gacaaacacc aacgcaaatt ttctcgctag cctttaccgc
     7621 tgccgtctgt ttggaattgt tctctttgat aggcgcttgg ttcatcgcag ccgtagaacc
     7681 gttatcacaa gggattaccg attttgccaa taaacgacta aaaggacgta ccatcaacat
     7741 aggtttagac tggcctttca tagcaggccg cgctgaaatc tgggctgccg ctaacgtatt
     7801 agcaccaatc atgttgctag aagccatcat cttaccagga aataaattat tgccactagg
     7861 cggaatcatc gcaatgggtg ttaccccagc actattggtc gtaactcgtg gaaaattaat
     7921 ccgtatgatc gtaatcggcg cgatcgaatt accattattc ttatggtccg gcaccttgat
     7981 cgccccgttc gttactcaaa cagctaaagc agtcggagcc tttccagccg gcttgaacgg
     8041 gtccgccatg atctctcata caacgatgga aggcccgatt gaaaaattcc taggttattt
     8101 agtcggtaat gcttctcaag gacaaatcga atttgtgata tacgctgcct tagcattagg
     8161 tgcatacctt ttaatcttca tttggtacgc tcgtcaaatg aaaaaacgca acgctgcata
     8221 cgcagctgaa aaagaagaga atgcatctcc ggcaacggcg cataatatga cttatgcagc
     8281 cgaagctaaa taagctaaaa acaccccgaa tgtctttaga cttcggggta ttattttacc
     8341 tataaaaaat aaatagatgt aaaatccatt tcaaaaagca tagcccatct cttgccacac
     8401 ccaataaaga aattatcaac gtttatgctt tttttaactc ctttaagctt gcctttgttg
     8461 gtattcctca acgattcttt ttgtgttctt gtttgttttg tgctttttga ctttttgttg
     8521 tttaattgcg aattccttga tttctttttt cctgaatcgc ttcgcatccc gcggttatgc
     8581 gtgcgatttc atcattgctt tttctgatct aactcgtttc atgtcaaaga gcttcagctg
     8641 acttagtgtc catggttgtt ttgatatccc ttttcctcct taataaagcg gtgattgata
     8701 ctgctatttt ttccttcaca taaacataac atcccaaaaa aatcgactat cttttttcaa
     8761 atggtgtatt caagtattcg gaggaattag taatgatgca ccctaaaaat aacataattc
     8821 gcttaaattc aaggctacca aaaaactacc aagttctaat tatctagagt ttggcagttt
     8881 ctcatttaca caacatattt ttcttctttt tcgatttttt catagtgctt ttgttcatca
     8941 tcatccgtta taactactgt tacatcattc agattataat atactgaaaa atctcttttc
     9001 ccaattttcg aatgatcaat caataaaaag cgttcaacag aattattcag agcgatctgt
     9061 tgagtttggc cctcttcaaa agtcgaagtc ataattttct gttcatttaa agcattacag
     9121 ctgaaaaatg ctttatgaaa attcatatcc tgtagcgatt tattcgtgat ttcaccaaca
     9181 aagcattgag tcgtttcccg catctcgcca cctaatagat agatttttcg attccctttt
     9241 cgcttattta attcttcaaa aacaggcaaa cagtttgtaa caatccgcag tgaatcccct
     9301 tgaatgattt ttgacatgag ctcgattgta gttcctggtc caagaaatat cgtatcacca
     9361 tcattgatca tttcattcgc cttttccaca atccgctgct tttcttcttg cagcataatc
     9421 tgtttttctc tatgagaaag ctccacatgc cgataaaaat ccttcaattt cgctccgcca
     9481 tggacgcgtt ctaataatcc ttgtttctcc atttcgtcca aatcacgtcg gactgtcata
     9541 tccgaaaccg aaaacttctc cataatatcg ctgactcgta ctgtaccgtt aacctctaat
     9601 aacgacatga tcgcttggaa tcgatccgtt tttaacattt catcgcctct tttcttttta
     9661 agactatcaa attcttaaga aaagtgctag aacaagtttt cttaagaagc cttctgcagg
     9721 aaggaaagca aaatttttca gaatttgcaa gctgctattc taaaaaataa aaccgaacga
     9781 gcatgactgt catagtgaca gccttgttca ttcgactttt atctttaata aatcatttta
     9841 tttaatctga agttattgtg caagttcaaa aacaaagtag ttaatgtctc ctcgatattt
     9901 cgaaatcgaa tactcaacgg gcagacgttc tagcgaaaaa ccctgtgtat ggaaatagaa
     9961 cattggatct ccttcttcca catctaacaa atcgctgatc gtatcatccg ctttgattac
    10021 ttctagtttt ctggtgaccg ttttaattgg ataacccatt tcttcaaaaa cttgatacat
    10081 tttttctttt ctgatttgtc aaattaagct agacaatatg tctttatcta tgcagctcaa
    10141 aaatacttcc aatggtgttc gataatttaa tgattttctt ggaatatggt ttcttttcga
    10201 ggcaacggat tgaataaagg tttcatccat gtccctaaag tccattgttt taggtaagcc
    10261 atctcgtcta agcaagccgt ttgagtgctc atttagccct ctttgcgaag gtgttcctgg
    10321 atctgcgaaa aagatggcaa tatcgttcga gttacttata gatcgccagt tggaaaattc
    10381 ttttccacaa tcaaatgtca tggatttaaa caagtgttta ggcatggatt tgaaccaatc
    10441 atttaacctg ttttcgatat cttcggctcg tctgcctgtc ggtttcaaag taataataac
    10501 cttggataaa cactcaacca atgtaataac agcgctcttg tgtttaactc ccacaatggt
    10561 gtcgccttcg agatgaccaa attctttagt caggtgtgga tagagactgt tccgttcatg
    10621 aatcgttctg cgaaaggcct gttttccacg cttttcttga tgattattcg gtttacgttt
    10681 ccccttcata ggtaacgact gtgtgtcaaa aaggcctttt ttaaacatcc gatagagtgt
    10741 acgaacagaa caatcaattg gaaactcgcc acggccgata atcacatcgg gtgaccaccc
    10801 ttgagctacc ttttcttgaa catatgttga ttgttcatca gataaaacgg ttgaacgtcg
    10861 tccacaacga gatttatttt gtttgtattg acggtaataa tctagcactt ttttccttgt
    10921 ttgagaaacg aataaacatt ataaatggtt tgtctagagc gatttaaact ttgtgcgaca
    10981 agagcgacag gtttctcgat attgaaataa gactctatga ttactagttc gtctgttgta
    11041 agatgggtgt aggtcatttg tactcactcc ttattaattc tttggtcgga actattttga
    11101 gtgtagcaca aatgatcttt ttagttgtct agcttaattt tacaatcgac gcctcaaaaa
    11161 gaatacccga aatttaaaca gcaaaaaaag atagcgctta ccattgtgtt taagataaaa
    11221 cgctagctaa gttggctagc gctttattaa tctaagcaag ctctaaaatt gcttcaatac
    11281 aaaatcaact gctcctttag gatcacgtgt taaattgata tattctgcac ctttagttgc
    11341 aattactttt atccctaatt tgtctgcatc tttacggaga tcatccaaat aggaattcac
    11401 ttgaggtgct agaacgatta aatcaaaatt tttcatcata tcataatgag aaccataagc
    11461 tcctgcttta gcagaaagat taacacctgt ctcttttgcg ccctcttcta atgcattagc
    11521 caacattgca ctcgttccgg caccagcaca aagtactaaa acgcgctttc cttcaccaac
    11581 cgatgctttt gcttcatcaa ctataactgc cgcagttgca ccaacttctt ggatttcttc
    11641 gaccaattct tcttttactt cttctgcagc aatgtttttt aactcttgtt ctactaattc
    11701 agaatcatag gccttaacaa aaggtaaata aataatcgca tcgacaacaa tcaatactac
    11761 cgctaataca atcgccaaca cactaaaccc ggttcctaaa accaagccaa tcggtgcggg
    11821 cgtcgcccaa ggcaatacgg atataaagct attcatcccc aagacatcga caaaaagttt
    11881 gaatatccat acgttagcga ttggtgctaa taaaaatgga attagaaagt acggattcaa
    11941 aataatagga gtagcaaata gtaacggttc atttaccgca aacgaaactg gaataaatga
    12001 agctttacca actgctttca attgttttga tttagcgaac aacatgaaca gaaaaggaac
    12061 aactagtgtc gctccagtac cacccatcgt tccaacaaag tttcctagac caactgttaa
    12121 tacttttgag gcttgtgcac cgtttttaaa taattcaaag ttagtttcta cgttcgcata
    12181 aataattgca gcaatcgctg gttcaacaat cgatggtcca tgaacaccaa caaaccagaa
    12241 cattgccatg gctccccaaa tgattgcaat accgacatat ccatcagcag cactaaataa
    12301 tggctgcaat aattgaatta cacctgctgc aaatgatgtt ccaaaaaatt gacgaactcc
    12361 cgtatcaatc aaaacggcaa taaatactgc aattgaaaag gggataatat ctctaaacat
    12421 ctgtgtgatt gttccgggaa cttcttttgg catacggatc gtcaaatctc ttttaacaca
    12481 gaatttatac acatttacag taacaaacgc agaaacgaaa gctgctaata atccctttgt
    12541 ccctaaataa tcagtagcta tcccaccatc aatttgatca gctgccatca ttacaaaacc
    12601 acaaattgac gctaacataa cagaaacttc attaatctgc ttacctgaat cagataattg
    12661 acggttcatt gaattagcta aacatcgagc tgttgtagca acaaccaaaa gaccaactaa
    12721 tcccattgaa taaccataaa ctttccaaag ccacgtcgag aatgcctctg gtaacgtcac
    12781 gttaaatact tctggaacag ccgcaatcat aataaaaata cttgaaaata gaatagctgg
    12841 catagcagtt aaaaagccat cgcgaattgc ccctaaataa atattacgtg agattttttc
    12901 aaaaaacggt tttcccttct caatttgctt aataatcgta ttcatttttc ttctcctatg
    12961 ctttagcaaa tttttctttg tacaattcaa caaaatcatt gataagttct cttaatagaa
    13021 tcgttgtcat taaatggtct tgcccatgaa taaaaattac gcctacttcc atttcgttcc
    13081 ctgacgcttc ttcagctaaa catttcgttt gtgagttatg cgctaaggtt aaattttcat
    13141 cagcatccgc taataatcgt tcaacattat caaaattacc ttctctcact tccttcaaca
    13201 ctgttaataa tgaacttctg gcatctcctg cataagcaac aatttcaaat cctaataatt
    13261 gcaattcttt cctatccatt tccttgccct cctaactatt tttatattat cgtttttgtt
    13321 tcgctaacaa atttatacca atatgcagac tctttcggat atcttttttg tgtatcgaaa
    13381 tctacataga atagtccata acgcttagtg tatccattaa cccaagagaa aacatccatt
    13441 aatgaccaaa tgaaataccc ctttacatta actcccattt tgatagcatc ggaaatcgca
    13501 ttaaaatgct ggcgtaaata atcgattcga tcatcatcta gaataatccc atcttcaaaa
    13561 ttatccttat accctagccc gttttcagta atgtatattt tattataatt tggatattcg
    13621 tcttggattc gtacaagtaa atcatataac ccttttggat aaatgatcca atcccaatcg
    13681 gttttcggaa gatcctcacg cacgattctc tcgccaatcc ctttaatgca atagacttgg
    13741 ctaccttttt ctccggtagt gttgtagtga agtgtgcttt ctcctttgta ctctttaacc
    13801 caatggctac catagttatt gataccaaga aaatcatttc gatttgctgc ttttttcatc
    13861 tctctaaaat cattagaatc aatatctgca gattgcccat ttgctgctaa aatctcatta
    13921 atatgtgcca ttgtttcatc tgagtaatac cctaaataag tagcatctaa taagaactta
    13981 attgataacg catcatctaa tttcgcagca tgcttatctt cttctttgtc acttgctgga
    14041 tattttgtct ctagcgagtg aacaacacca atttcccctt tatatccacc atctttaaaa
    14101 atgtttactg tctttgcgtg cgcatacatc atgttgtgta aacaagttac agtcttctct
    14161 atatcaaatt gaacttctgg aggaaacgat ccgatcaaat attgatttga agcaactgga
    14221 taaatctcat taaatgtact ccaataatga acctcagaaa attcatcgaa acagaactgt
    14281 gcatagttta cgaaatgttc aacattttct cgatttaaaa aatctccatt atcaaatagt
    14341 tctttagggg tatcaaaatg atgaagagtt acaaaaggaa taataccatg cttattcaat
    14401 tcagcaaata ccttatgata atattccact cctgtttggt tcacttcccc atagcctttt
    14461 ggaaaaatac ggctccatgc aatagacatc ctaatactat caattttaaa ttttttgcat
    14521 aattcagcat caactggata ttgattataa aaatcactag ctggatcagc agtatagcgt
    14581 cccttttcta caaggaaagt atcccacgct acttgccctt ttccaccttc ttttgtagag
    14641 ccttctgctt gataagctgc cgtagctcct cccataataa agtcttctgg taaagtttta
    14701 taattcattt taatctctcc ttgaaaatta gtttgatcaa tatgccaata atccataaat
    14761 cacctcagca taattttaac atgtataagt aaattttttt acacaacgaa aaaccgacct
    14821 cgaatatcta ttttattgcg atatacaagc acttatatcc cttgatgtaa aataaatata
    14881 ccattcaaaa accgttttgt aaacgttttt ttgtaaaata aatttatctt tttactaatt
    14941 taatttacac gaaacaagaa aacggactga ttcccactaa aatcaatccc acctactaat
    15001 cattctccaa gtggctatcc attatcgatt caatcttctc tgttaatgga ttaggctctc
    15061 tgctgatatc agagtttcgt ttcctgctag aaaaagtaat ttaagttact aaaagagcag
    15121 tttcgattta ctttaacaaa gaaaaggatt attatatccc taggaggatg aacaatggtt
    15181 actattagag atattgcaaa aaaatctggt tactcaacgg ctactatatc aagacttttt
    15241 aaaggtgatg agtcactctc tattacatct gatactaaaa acaaaataat cgttacagcc
    15301 aaatctttag gatatgatct gacaaaaatt aagactactc tgtacaagat tgccattctt
    15361 ttttggatct cagaggaaca gttaaaaaag gatgtctact atcaacatgt caaagatgat
    15421 ttgaaaaatt atagtaaaaa agctaatcta gatttgattt tcatctctaa aaacgatggt
    15481 gtaaaaagca ttcctgacga tattactgga tttatcgcaa taggacaatt gacaaaaaag
    15541 gaattgtact acttaaatca aaaagggtat aagggggtaa tccgaggaat aaacccatta
    15601 cctgaacact tcgatactgt agaacctgat ttccacgaaa tggccagaca agcaattaac
    15661 tactttatta atgaaggttt cacaaaaatt ggatttattg gaggaaattt ctttaaccta
    15721 gaaacgggta aagaagagat tgatagccgt gaatatgcct tccgatatca tttagaaaag
    15781 agcggactac tcaatgaaaa gtatattttc tcccaaggat catttactgt tgacgaaggg
    15841 tatcaattaa ctcttaaaat gaaagctgag ttaaatgaag atatgcccga ggcatgtttc
    15901 ataccatctg acactatagc tgccggcgtt cttcagggct gccatgaatt gggtattaac
    15961 atccctaaag atatggcagt aataagtatt aatgatgacg acatcgcaaa attcgttgca
    16021 ccaccactta caacttttaa gatagatatc gaagagatag ctaaaacctg tgtagattta
    16081 ttaagtgatc aactggtata ttcgcgggac atatcaaaga aaacattaat cggcgcaaaa
    16141 ttgaccgtta ggaaaagttt tatccctaaa gaagaggtgt aaaataagta attgaaactt
    16201 atctttaaaa ctataattgt gtccacatag tagccgagca atcgggttta tgcctattat
    16261 gttttccata aataatttga tggtgatatt ttgacgtaaa tacaatatga ttttttcatc
    16321 gccaaattgt gcgcgctaat ttttgtttat cattcagaac atttcctcct ttttatctaa
    16381 gtatgaggtt ggataaacgc ttcatcttag tatcttgggg gattttttat ctcgttggga
    16441 atatatcaag catcagagcg cgcaaaaaga actggtcgaa aaatcaatct accagttcaa
    16501 tctcattcac tgtgatacta tgttttatac agataacgcc atattataaa acaagttgta
    16561 ttccgcaaat gggttctatt caactcttaa actatctata aaagattcag ccaactccat
    16621 tagcctctcg taatttagtt ggtgaacagc acttttttca aaaaattttg aaccaattcc
    16681 tacataagag gctccattgt tcaaatagtt ttgtgcatta gtctgatcta cccctcctac
    16741 agccatcaaa ggtaaacttc ctaaaggggc ttgcacttga ttgaaatatt ccggtccaag
    16801 acagttggcg gggaacacct taacaatatc agcacccatc tcaaacattt tgaatatctc
    16861 tgtgggcgac atggccccag gaattgttag aaggttctgt tgcgaagttt gaaaatcaaa
    16921 cgcgccctct ctgaactcca aatatcttag gctgtta
//
LOCUS       DAKBVG010000040        16910 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000040 DAKBVG010000000
VERSION     DAKBVG010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.040"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(420..3125)
                     /locus_tag="OUD09_000957"
     CDS             complement(420..3125)
                     /locus_tag="OUD09_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HCT9692240.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(3431..4378)
                     /locus_tag="OUD09_000958"
     CDS             complement(3431..4378)
                     /locus_tag="OUD09_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HCT9692241.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            4568..4837
                     /gene="rpsN"
                     /locus_tag="OUD09_000959"
     CDS             4568..4837
                     /gene="rpsN"
                     /locus_tag="OUD09_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCT9692242.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(5013..5375)
                     /locus_tag="OUD09_000960"
     CDS             complement(5013..5375)
                     /locus_tag="OUD09_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCT9692243.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(5386..6507)
                     /locus_tag="OUD09_000961"
     CDS             complement(5386..6507)
                     /locus_tag="OUD09_000961"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCT9692244.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(6523..6873)
                     /locus_tag="OUD09_000962"
     CDS             complement(6523..6873)
                     /locus_tag="OUD09_000962"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCT9692245.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(7003..8514)
                     /locus_tag="OUD09_000963"
     CDS             complement(7003..8514)
                     /locus_tag="OUD09_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCT9692246.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(8836..10188)
                     /locus_tag="OUD09_000964"
     CDS             complement(8836..10188)
                     /locus_tag="OUD09_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCT9692247.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLAATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(10341..11417)
                     /locus_tag="OUD09_000965"
     CDS             complement(10341..11417)
                     /locus_tag="OUD09_000965"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCT9692248.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            11613..13358
                     /locus_tag="OUD09_000966"
     CDS             11613..13358
                     /locus_tag="OUD09_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HCT9692249.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(13638..14075)
                     /locus_tag="OUD09_000967"
     CDS             complement(13638..14075)
                     /locus_tag="OUD09_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCT9692250.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(14059..15432)
                     /locus_tag="OUD09_000968"
     CDS             complement(14059..15432)
                     /locus_tag="OUD09_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9692251.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(15754..16242)
                     /locus_tag="OUD09_000969"
     CDS             complement(15754..16242)
                     /locus_tag="OUD09_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCT9692252.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(16264..16866)
                     /locus_tag="OUD09_000970"
     CDS             complement(16264..16866)
                     /locus_tag="OUD09_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCT9692253.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         4750 a         3612 c         3074 g         5474 t
ORIGIN      
        1 acattttttt atgggctttt tctttgaatt ctataataaa aggatagaac ttgacttaga
       61 aaatcaagtt ctatcctttt tgatatctta acttttggaa gagagatgta aggctgcttt
      121 ctacccatca ttcttactac tcgaagcata gcgcagttgt cacaacctct tgataaacgg
      181 tgtttaaaag cttgcatctg cggtaataag ctcaaccaaa ttaaaaacat aagaagctgt
      241 ttttatttgg tcgttcttat tacttgatgc agagcagctt ttttacaacc tcttggtaca
      301 cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa acggcaaaat atgaggaact
      361 atttttccat ttttttctct aatactcagg tcaaaacact gatttcacaa cctctttttt
      421 taatgaaaaa tatggctctc atgcgccgct tctttgaatt tcgtcatttt ttcttcgatg
      481 aacggataga ttgccgttcc tacgattcca aagacaacaa acaaaccgat catgatccag
      541 attgctttcg tagcatttgg ccaataaatt cctcctgcag attcccgtaa caagtttacc
      601 gcatgagtaa atggcaagaa cggattgatc atttggaaga atttccctga gacttggatc
      661 ggatagttcc cgcctccgcc agagatcgac agtactaaga tgataatagc gatcccttta
      721 ccgacattat cgaataatgc agccaagaca tagacgatca tcatgaacgc taaggcgatc
      781 aagacagcaa atgacacgct gtagaccgga ttttggacat ctactcctag taagaaaata
      841 ttccctaatg taacgatcaa gctttgcgca atcgccattg tcaaaaatgt cagcatacga
      901 gcaacgaatt gttctcgttt cgtaaaattg tttcggtctt tctttcctaa atagtattct
      961 gtcgtagcaa cacttgaaag aagaacagcg ccgacccaca gacatagtgc agtataaaac
     1021 ggggtgctag ctgaaccgtt atttgcgatt ggataaagcg cattggtttg tagctcaaca
     1081 ggttgtgtaa agaagtcact ttcttttgtt gcgtctagtt tcaataattt gatgacttcg
     1141 cctagatcga cttcttgttc gccttttcgg atcgcgtctg ctgctttttg gataccagct
     1201 ttgatatttg gccagtcatt gttgatcaag tccgcagcag cgttcacagc tgtttcgact
     1261 tctggcattt tttcatttac tgttgtcaat gtaccggtga tttcattttt tagtgctggc
     1321 caatcatttt ggataaaatc agcggccaat cctaattttt gctgaatgat cggtaattca
     1381 ttgttgtata ggtctgcccc ttcattgatg ccattgacga tggtatccat atttccattc
     1441 agcattgtat tggcatcatg gatttcttgt ccaattgcag gcatttcttt ttgatatttt
     1501 tctaaaattg ccactgcatt cgttacagtt gctttcgttg agctgagaag cgaatcaaag
     1561 tcgatctggt ccgctttgct cagtgcgcct tgtgcagtcc caatcgtatc gatgacttgg
     1621 tctagtagag aagtgacagt ggaactgatt tgacctgtat tgataccagc aatgatgcca
     1681 ttgatattat taacaacggc atcaatttga tctagcaatt catcaatagc cttcgtgtct
     1741 tcattttgaa cagctgtgtt caaagcagat aggcggtttt gcagattgct tagcaagggt
     1801 tttaattgat ttagctgatc aataattggc tgtaatttcg tatttccagc acttgtttgc
     1861 aatttagtca ggaactcgac cagtttatcg attgcatttt gttgttgaga tattccgtca
     1921 ataaagccgt tcaatacatt gctgatgttt ttcttttcat catcagtcag cttaccatct
     1981 tcaatagctg ttcttacagt atctattaac gaacctgtcg ttgttgccac ttgtccaatt
     2041 gacttcaatg taacatctac actgtttgtg atgcttggta tcgcatcttt cagctgagag
     2101 gcacctgatt ttgtttggtt tgctagatca cttgcctgat tttctaaatt tttcacatct
     2161 ggcaagatat cttgtacttg ttggatgatc gtcaagcctt gttttgcttc atcgattcct
     2221 tggttcattg tctcttggat cgaagcaaaa tcgccgtcga tctctgctag ttgcttaccg
     2281 gcattttgga tctctgggat tttttcttgc aaatcaagga tgatttttgc ttgatctttc
     2341 aactcaggca ttttgtcatt caatgcaacg actttttcac ccatctcatc tactttaggg
     2401 atgtaatcca cgaattcatt tgctttattc agtttttcct tgatttcagg tagttgactt
     2461 tgtaattcga ggacttgttt tgtgtagccg tcgatcgtgt ccaaattttc atctgttgat
     2521 aaaatcatat ctttaacttt attgatactt actaggttcg tatcgatgtc gtaacctatc
     2581 tcattgaata ctttcaataa agtcgaactg gctgttttga taaattcatt tgtgatttgc
     2641 gattggatcg aacttgctcc tttatcggtg attttcggtg caatcgcatt gattttttca
     2701 tttaccgtat attcgatttt tggtttcttg atatcaccac tagtaaaact cagcagatct
     2761 tctgagaaat cctttggcaa gtaaatacct gcgtaatact ttccggaacg gactccgtct
     2821 tctaattgtt cttttgaatc aacgaattgc catcctaatt gtttattttt atgaagcgat
     2881 tcgatgactt gctccccaat ggccacttct ttaccttgaa actctgctgg cttgtctgca
     2941 ctgtaaacag caattggcag ctctccagta tttccatatg gatcccataa tgctttgatg
     3001 ttaaaccacg catatagcga agggatgatc atgatcgcga tgatcagaag tgttgcaatc
     3061 gggtttttga aaatccgttt ccagtcaagc aaatataatc gaagcgtatt tttgattgac
     3121 tgcatagttc tcccccttct tcttttttca gctgaattac tttaccatgt atttcatact
     3181 tttttcaatc ggcttttttc aaaaaagtcg aatttttgga catatcacgg caattgtctc
     3241 attgtaatct cttacatatt gagaaataat taaggattac agcctttact ttatcatgaa
     3301 ataaaaggga aagataattt tatgccaaag tttttatcta ttatacgtat acgcaaaaaa
     3361 gctaaaacat tttttgtctt tgcccctaca aaaatgtttc agcttttttc gttttttatt
     3421 cctgtatttc ttatttttcg aatgacagga tgattttccc agcaggagga tgtccttcaa
     3481 ctagctgatg tgcttggatg acatgcttaa gtgaagcaga cagattcatt gaaataaaaa
     3541 catgaaaggc tcctttttgg taggctttga ttatgccagc aaaggcttct gcatctagat
     3601 attcctttct tggaacaaac gattcataaa agccttcctt tttttgccgc aaatctagat
     3661 ctgggaggtc atttaatgca acatacctgc cgcctggttt catgatctgg atgccggttt
     3721 ctcctttgat acttcctttc gtcgcatcga ttacaaggtc tgcctgatca gcaaattgca
     3781 gacctggatt tgttttatca taagcagcaa atgctgaagc tcctaatttt tttacctttt
     3841 cctcattttt actggatgca ctggttaaga ttcggattcc cttttcatgg agaagctgga
     3901 tcagacttga cccgaccgca cctgatgctc ctagtatcat tacagtatct gtcggctgga
     3961 tttccgtcaa atggttgatt agattataag ccgtaattcc tggcgtgacc attccggctg
     4021 cttcttccca actcatttta tctggaattt ttgctacttt tgctgatggc aacacgactt
     4081 cttctccgta agtcccgccg actgcatgga cagctactcg atcacctaca tgaacagttg
     4141 tgacatctga accaatctct gtcacaatac cagcgccatc attacctggt acataaggga
     4201 atttcaatgt tcgtatctct ttcatttctc ctaaacgcaa ggcaacatca taaggattca
     4261 cactgaatgc ttttatttct acacgaacat gtccagaacc gacttctctg ggatgagcat
     4321 cgatttcttc aaaaacatct tgtgcttctc cgtaaccgtt aattgcaaat cttcgcatgg
     4381 tcaccatctc cctttttctt taagtttacg tttatgtctt tattttgaca aaaaaaatgt
     4441 cttttcgttt tggcgcatga ttcaaaacaa aatagttgtc ttttttcgcg taacgtgtta
     4501 aattatttat ctaaaagcgt aaagattacg atttacgcta ataaaaaata attggaggaa
     4561 tttgttgatg gctaaaaaat caaagattgc caaagcaaaa aaacagcaag aaacgattga
     4621 acgctatgct gctttacgcc aggaattaaa agcgcaaaaa gattacgaag gcttgcgtaa
     4681 acttccttta gatgctcgtc cagaacgatt gaaaaatcgt gatttaatcg atggacgccc
     4741 tcgcggatac atgcgtaaat tcgggatgtc ccgtataaaa ttccgtgaat tagcacataa
     4801 aggtttgatt ccaggagtaa aaaaagccag ctggtaatcc aagtctggct gtaatgtaaa
     4861 ggaggagggc gcatggacca ctccttggat catcaaaaaa gatacgttcg cttcttctag
     4921 cagaaatgct ggtactagaa gcagacgtat ctttttttac gcacagctgg tcctttttta
     4981 tatgtaaagg ctgcatggtt acttcgctat atttatttag taacaggagt cagtgcttcg
     5041 atacccacac taattttcaa tgctcggtca acttctgcca taatgggtcg atccaaatgg
     5101 cagacctttt ctttcaatct tgatttatcg atcgttctga tctgctccaa caggataacg
     5161 gagtctcttt ctattcctgt tccagtagct ttgatcccaa tgtgagtcgg aagcttaggt
     5221 ttagccattt ttgccgttat ggccgcaaca atgatcgtcg ggctaaaatg gttacctaga
     5281 ttattttgaa taaccaaaac tgggcgtgtt ccgccttgct ccgatcccac gactggagaa
     5341 agatcagcaa aataaatatc tcctcttttt accataggat cctccttatt cttgatattc
     5401 tcttggtatc cgctgtccta atccgcaagc cacttcatag tggatcgtac ctaattgatc
     5461 tgctatatcc tgcatagtga tttctttgtc tgcatttttg ccgataagcg tcacttttgt
     5521 tccgacagga aattcttgag gcaatcggat catcagttga tccatacata cacgcccaat
     5581 cgtttcacag tagttccctt ctaccaatag agaaaaaccc tgcatctttc gtggccagcc
     5641 atctgcataa ccgatcggta tcgttccgat ccactctgct tcaggagtga tatatgtttc
     5701 tccatagccg attccttcac ctgctgggag ttttttgact tgaatcaatt cagaaaccag
     5761 ctcaagtgct ggctgcaatg gataaacttc cggtagtgcg tgcccagagg gattaagtcc
     5821 gtacattgct actccataac gaatcatatt gccgattgtt tcatcatgcc ataaagccgt
     5881 tgcactattg ctgacatgta catatcgagg aagttcggga agttttttca gcacttctat
     5941 aaagcgttct ttttgcagat tccaatatgt atcatctgcc tgatctgctg tagaaaaatg
     6001 ggtaaataaa ccttcccatt ctaatacacg actttctttg atgaatgcag ctgcctcttt
     6061 tacttcttca ggtgtacaaa atccgatacg acccatccca gtgtctgctt ttagatgaat
     6121 cgaaagaggc gtctctagct tacttttctc taaaacagca gctgctgctt gcaaccattc
     6181 ccgtgtacct gcagttactg ataaatcatg agcaacagct acagtcagtg cgtcgtaagg
     6241 aaccatattc aagataagga tgggctgagt aaatccagct tctctcaact cgactccttc
     6301 atctaaattt gaaacacaaa agcccgaagc tccacctgca actgctgcct cagctgtttc
     6361 aatagctcca tgaccgtatc cgttcgcttt tactactgca aacaattctt ttccttgcgg
     6421 aagtcttgca acttcatttg ctacattttc agttatagct tttttatgta tcactgcttt
     6481 cgttgggcgg tgccaagcga caaccatcgt ttttccttct ttctagtctt ccaaaataat
     6541 ttgtgcaaaa gctgtttcat ttgtatgagt aatactgacc catacattgc cttcatgagg
     6601 tgaacgcgtc acaacaggct gaccattttc attttttaaa atctcgacat cttgaaaggt
     6661 aacttttccg atcccagttc cccaagcttt agagaaagcc tctttgcaag cgtaacgccc
     6721 gcctaaaaat tctacttggc gatgaaaagg caaggactgg aatagtttca tttcatcaga
     6781 ggtcaagatt cgtgcaatga attttggttt ttcttcgatc agccgagtga tcctaggaag
     6841 ttcgactgca tcgatcccaa ttccttttat catatgcttt tctcttttca ataaatttag
     6901 aacactctca ttctaccaaa ttataaccat tttgccacaa ataagtccac aaataacaaa
     6961 aatgaaagtt tctcttaaaa aagagaaact ttcattttat gtttaatcgt tatttgaacg
     7021 gattacaaat ccgcgttttt tatcattgtg acgtttaccg ccatcttttt ggctgttatt
     7081 atttttgcta taaccgcctt ttgatttgtt tccacgataa ttaccgccgt cacgacgatt
     7141 acggttgttt ccgccaccac gtttgccatt tttattgtat ccttttttct gcattggcaa
     7201 tgggcgttct ggtgtgatct tcactggcac tgcatcagct ggatcttttg ctgtcgtttt
     7261 tagcaaaagt gcaactaaat cttgtgcaga atattcttct aataatttat ctgctgtttg
     7321 caggtattta tctagaccat tttctgcaag ttttgtttcg atttgttcaa ctgcagcacc
     7381 taattgacct ttgaatgctt ctttttcggt tggtggacgt aaagtcgtca tccgtttttt
     7441 cgtcaagttt tcaatcacat gcaagtagtc catttcattt ggtgtaacga aagtcacaga
     7501 cataccgcct tttccggcac ggcctgtacg tccgatacgg tgaacgtagc tttctggatc
     7561 ttgagggata tcatagttgt acacatgagt gacacctgaa atatctaatc cacgtgcagc
     7621 aacgtctgtc gctacaagga tatctaaatg accgctctta aatgaacgca atacgctcat
     7681 tcgtttttgt tgagacaaat cgccatggat tccttctgca cggtaaccgc gtgcttctaa
     7741 tccgcgtgcc aattcgtcta cacggcgttt tgtacgtccg aagacgattg ttaattcagg
     7801 tgtttgaaca tcaaacaagc gagtcatgat atcgaatttt tcgtattctt ttgcgcggac
     7861 atagtattga tcgatcaagt cagctgtcat ttctttcgct ttgatcttca catgatgtgg
     7921 atttttcatg aatttaacgc caatattctt gatagctggc ggcatcgttg ctgagaaaag
     7981 taatgtttgt ctttgatcag gaacttgtga aatgattttt tcgatatctt ccaagaagcc
     8041 catattcaac atttcatctg cttcatccaa aactaatgtt tgaacggttc caagtttcaa
     8101 tgtgtggcgg ttaatgtggt caagcatacg tcctggtgta cccacaacga tatgtggacg
     8161 atctttcaag ccacggatct gacggccaat atctgctccg ccgtaaacgg cttgtacacg
     8221 tacttttttg tcgcgcccta aacgatataa ttcttcttgt gtttggatag ctaattcacg
     8281 tgttggtgcg ataactaaac cttgtaattc gtgacgatct ggatcgattt tttctaacat
     8341 tggtaatcca aatgcagctg ttttccctgt acctgtctgt gcttgtccaa taacgtcttt
     8401 tccagctaat gctagtggaa tcgtttctgc ttggataggc gttgcttctt caaaaccagc
     8461 gcgttcgact gatttcaaca attctggtga taattctagt tctttaaatt tcaaatgaat
     8521 cctcctatat ggttgttaac tgaggatgta gcttttttgc taccccgtcg atttgttcgg
     8581 tcaaagtgta ttgtttcttt ttgacagtgg ccacgcacgt ttttatccat tcttagcccc
     8641 tgtggcacca ccgaaaaatc actttgcgaa gtttaggcat tagaaaagcc aaacgggtcg
     8701 cgttcagctt tatcattatt atgacatctg gcttcaacaa gttgtttcta tggcaaaacc
     8761 agtccaattt ctcttcatct ttcggcaata ggttattcta acatagtttt tcgttttctg
     8821 caagtttaca gatttttatt tcatttgctg aagttgttct accacttcaa ataagcccat
     8881 gctgttgctg ccttttagca taatactatc tgttgctgca agattttgtt tgatagcatc
     8941 aatcaattta ttcttttctt tttcaaaata gaacacaggt agttgggggt atttttggtt
     9001 caattcttct tttagtgcat gcatttgttc cccataaagg aaaatcatgg cgaatttctc
     9061 aggatcgata tgttcactca tttgcgcatg catatttaaa gactctttcc ctaattccag
     9121 catatctgct aacacagcga ttctgcgtcc ttttgttggc agatttttaa aagtatctaa
     9181 taccagtccc atggctgttg gatttgcatt gtagacatcg ctcaaaatag ctgcaccatt
     9241 tcctgccgtt agccattcgg ttctgttttg agtgatagaa acatgggcga gcccttggcg
     9301 tatctcaggt atggacagtc caaagaagcg cccgatcgta tacgcaatca aagcattcgt
     9361 tacattataa cttccaggca gcgggatcgt aaatgtttct ccttctacta caaaatcggt
     9421 ttgtgctttt ccttttgctg tcacttccgc atgaacatcg ccatttccaa taccgaatgt
     9481 tgtgactgtt tgtactaatg ttgccgttaa aggtgtcagc aacggttcat ctgcgggcac
     9541 aacgagtaat ccgtctgata caagtccgtc agtgatttcc attttggctt tagcgattcc
     9601 ttctcgcgac ccaagatttt ctatgtgtgc ttcaccgatc attgtgattg ctgcagcttt
     9661 tggttgagcc ataagtgaaa gttccgtgat ttcattggcg tgatccatgc ccatttccaa
     9721 aacgatcttt tctgtatctt ggggcatgtg caaaatcgtg taaggaagac caatattatt
     9781 gttgtaattt ccttgcgtct tatatgtttt gtatttttca gacaagacag acgcggttag
     9841 atctttcgtg gtggttttac cattgcttcc tgtaattgca acgacttctg cgcctacttt
     9901 ttccaagtaa taggttgcca attgctgcat ggctttcaaa ggatctgcta caggaataac
     9961 tggaaaattt tttggagctt gttcaacagg caagctccaa aatgaagcag atgctccgtt
    10021 tgtgatcgct tgctggataa attcatgtcc atctcgcgta ccagcaagag gaacgaataa
    10081 acttccttcg gttatcttcc ggctgtcaaa ttcgacattg gtaatctttg tcagtgcaga
    10141 gcattcgctg cccacggctt gtgcaatttc ttgtattgtt agattcattg agatctctcc
    10201 taaaaaaggt tgtgaaaaaa gcgtttggcc taaacggcgg gttcgcatga ctctcacttc
    10261 caagtaaatt tctctattcg tatttagaag ctagaatgaa cgcggttacg caatcactcc
    10321 agcctcttct ttatttctct ttattcattt ttttcaaaaa aagattgacg ctgatggtat
    10381 cgattcattc ctaactggat cagttcttca atcaaatcac cgtatttcaa gcccatattt
    10441 tcccataaga gtgggtacat actgaactcc gtaaatcctg gcatagagtt taattcattc
    10501 aggaataatt catttttatt tgtcaaaaag aaatcgcacc ggctcaatcc gcttccacct
    10561 aacatcgtgt aagctaactt cgcgtactct tgcgcttttt gataaacttc ttctggcact
    10621 tcggctggaa tctgcatttc gattttatta ttgatatatt ttgcttcata atcatagaat
    10681 gctacgtctt ttacgacttc accaggcaaa gtcgtccgaa cgtcttcatt tcctaataca
    10741 gcaacttcga tttcgcgcgc ttcaattcct tgttcaacga ttgctcgaga atcatactga
    10801 taggctgttg ctaaagcatt ttgcagctct tctcggtttt ctgcttttgt aatgccgaca
    10861 ctagaaccca tattcgccgg tttgacaaac atcggataaa gcaaagaacc ttcacattga
    10921 tcaaatactt ttttaggatt ttctttccat tgattcttaa gtactggtac ataaggaact
    10981 tgcggcacac cagcagcttg taaaatatac ttggtcatga ttttatccat ggcacatgca
    11041 ctggtcaata cgcctgcgcc gacataaggc atattcaatg tctctaagaa gccttggatc
    11101 gttccatctt ccccgtttgg cccatgtaaa actggaaaaa cgatggctcc ttcttctttg
    11161 atttcgcccg gattgatcac ttttcctgta aagccttcct ctgtctgtcc acttgggtcc
    11221 catgaaagat gcaagacatc tttgctggca ggtttttctg ttagtaatgg acctttgacc
    11281 cattgtcctt cttttgtaat aaatacgagt tgaacttggt aataattata ataaatggca
    11341 tttaaaactg aaaatgcgga aagaatggac acttcatgct ctgcgctgcg tccgccatat
    11401 agtaaagtaa tcttcaaaag attgtcctcc tgtattctaa atcgttcagt gatttactct
    11461 catattttag cacaaaacaa aaataaacga ataacttatt tataaaaaaa cgagccagct
    11521 tctaaaagaa cgagcgtcat ttattttttg tcagctagat tatttgtccc gttttttata
    11581 aaaagttatg ataaagaaaa ggagggatga tgatggcgca aacaaatcca cgcagccgct
    11641 tggcagaaat cattcgagtg ttcatcaatt acaatgtagt gccaaacttt gtacagcaaa
    11701 aaaatcccga acaggtaaaa aaagcctttg aagaactagg ccccaccttc atcaagatag
    11761 gacagatgct ttctgttcga gaagatttgc tttcttctgc cttcactcaa acattcaaaa
    11821 cattacaaga tagtgtacct agcgacacat tttcaacggt caaaaagacg atcgaaacag
    11881 aactttctct ttcattatca gatatattcg atgatttttc aaaatcgcct tttgcctctg
    11941 cttctatggg gcaggctcat cgtgccaagc tgaagaacgg tgacgcggta gtcgttaaga
    12001 tccaacaccc taacatcgcc gaagagatcc gacttgatct tcaactattt gaacgagcga
    12061 tccctttgat caaatacatt cctgaaacta gcgtagttga tctaaaaggg gtactccaag
    12121 aagtaaaacg ttccttgatc aatgaaatgg acttcttaaa agaaagtcaa aatggcgaac
    12181 aattctatca aaagaacaat ggctggaaag agatccgcag ccccaagatt tatgacgctt
    12241 tttgttcgaa aaaagtgatc gtgatggaag aaatgtctgg taaaaacttg aatcatttaa
    12301 tgaacgccga aaacaaaact gaaactttta tcactggaat tcaaaacaag cagctgaaac
    12361 aagaagttgc aaagctttta gttgagaact ttatgaaaca ggtgtttgat gatggttttt
    12421 tccatgctga tcctcatcca gggaatctgc tattccatgt actaacaaaa gaagaacaga
    12481 cacaagctag ccgaaaaact gaaacagtcc atgaaaaaga gttcggttcc tttgcttttc
    12541 gagcctccac ttctgcagaa gaccctgttg cgccatatac catcaactac atcgattttg
    12601 gtatgatggg gcatctttct gcaggactta ggcaaaaact cacacaagcc gttttagcgc
    12661 tgtatacgaa agatgcttat cgaatcgaaa aagcggtcct tcgactttgt cagcaagaag
    12721 gctcgtttga tgaaagtcgg ttccaccaag aactgacaag ttttcttgaa cagtattatg
    12781 attctcctat tgatgaaatc aacttgcaag aagtattcac acatgtcgtg acgatttgtc
    12841 atcaaaataa tctgcagttc gatcgtgata tcacgctttt gctgaaagca tttgggacat
    12901 tagaaggggt gatccgcgtc ctcgatcctg aagtttccct catggaagtc gcttcgcctt
    12961 ttgctcaaca ttattttctc actcatctgg atgtagaaga tacattgaaa caaagcggat
    13021 tagacttatt ggaagggatg aaggctgcgc ctaagatccc tcaacagctc caccatctat
    13081 tagaaatgtg gacttcggga caaggaaaag tcaatttgga attgaaaaag caagacaaat
    13141 tattgagcag gatcgaaagt atgatcaatc ggcttgtttt cggtatgatt ttagctgcat
    13201 tgatcgtagg gtcctccctg cttgtccaag cagcacctgt agaaaacgca gaggtcgttt
    13261 ccttattagg gatatttacg tatgctattg ccgcattcgt cattattttt ttggcaatcg
    13321 atgcattgat ccaaatgtat aagaagagga aaaagtagaa aaaggttgtg acagaaagaa
    13381 tcagcttcaa gaaataagaa ggggttttac ataaattgtt tctcaaattt ttgtgaaaat
    13441 tcggcttatt tttcgaagag gctacttctg aaacaccgag aataaaaaat gtgaaaaagc
    13501 tgttctgcac ccagtaataa gaacaatcaa aaagggaaca tggaacacat ttatcctgtt
    13561 tccctgttcc ctttttgatg tattcgagca taaataaaat accttcgtta cctactatct
    13621 tctgtacttg gtaaacctta gtcaattgcc ttttttaacg cttgacgaaa agacatatga
    13681 tctttctccg taaagggttt tggacccttt gtccttatgc cagcttttct taactttcca
    13741 ctatgataac gttgttctag aagcgtgccg atattcgctt cgtcaaattc ttttccagag
    13801 tgatggaaca ctcgcacacc cttcggcaat acaagtgaag ctaaaccata atcttgcgtg
    13861 atcaaaaagt cattcttctt aaccataccg acgattttat aatccgcact atcagctccg
    13921 cggtctacgt agacgatttg gacatgatta gcgtattctt tggtcgtata atggtccaca
    13981 ctggtcacga taaccacgtc aagaccatag gattctccta attttatgac atcttctttt
    14041 actggagaac catctccatc aatgaatagc ctcattcttt ttcgccttct tccataaatg
    14101 ataaacagtt actaatccgc tcagtaaaag accaacattg atccattttg ccaagataag
    14161 cagttgtaca ctttctatcg gcactgattc atggttcagc gtgatcaata atagatggat
    14221 tggctctccc atttcttttg ctaaaacaaa caaaagcgtc atcaataaaa actggtggat
    14281 cacactccct ttattttgag tagtatactt gacaacgatc cggcgatcca ttggaatagc
    14341 tgtatcagtt tgataatatt ccttatttag ccagctgcct gttactacgt gccagaaact
    14401 aagcagaaag acacctatcg caaaaccatt tggaatcaag aggaccaata aagaagaact
    14461 cagtccaagc aattgcatag ccaatggaga ccatgttaaa cgtttcaaaa tccatggtcc
    14521 gactattttt gctgcaatca agcctgctgc ataaggcaaa tacataaaaa tcgaaagttt
    14581 ttctaatccg tacagtatgc caacataaaa actgctaaat aaaaaaagga aattccctag
    14641 cattccattt agaaaagtaa acagatttag ccagcttggc aacttccatt ccttcttctg
    14701 gtgtcctaaa acaaacacga acaagatgaa cagcagcaag aaaccataaa tagcaagatc
    14761 aaaaagcctt tcatcgaata acaatcgggc atttctcaaa agaaacaata agccaaagaa
    14821 tatcaaaaat aaaacaagct ctcgcgtagc taccagattt cttttgatgt ctttgaaatc
    14881 cagttcgtaa cgcggataat gactaaccgt atggtaggcc atgacgtaaa aaagtgtata
    14941 aatagtaaag cttgctgcaa tctgagtcgg tgcaggcaac tcaatcgctt gatacaggag
    15001 aaatagccag atagctgcaa ataagtactt tttcccatcc atattgataa aaccttgcgt
    15061 cttttcgtga aaattgaccg tggtattagc aggtacaagc catgctgcac tcagccctag
    15121 caaaacactt gaaaaataaa ataaaggaaa ataaaatacg cctaatattc caagcaatga
    15181 accagcacca ccaatcagca gagcgatcat cagcaacgtg taactatcta gaccaaatcg
    15241 tagactgttg actaaaaata gtccagtcat ccgaaaggca taaaacagcg taaatggcaa
    15301 aatcgtcagc caattatgcg agtctgttag tcccaaaaat aaaagataag gaataaaaat
    15361 aaccgtgtta cttaagataa agggcgttgt ttgtgtaaaa ttacgcccaa atcgtttgag
    15421 tttttcgaac atgacaatgg ctccttaaac agtcttataa ctaatctgct cttcgatttt
    15481 agcattaaag gggaaataaa ccaattttac agaatatatt cttgtaaaaa aaaatacaga
    15541 aagaagcata agcttctttc tgcattttta cggtacttga ttactcaagc agacaagccc
    15601 ttctccacaa aagtcttgcc cgacacccct gcatccggca ggtgataaag atagaaatca
    15661 attatcctgt gcctccaaaa tcaattagga gctctgtaaa ccagagtcct tctcgacccg
    15721 tcgcataaat ttactttaac acagttccta tgtttatgcg agagcttttt tcattttata
    15781 atcgaccatt tgaaatcctt gaccgatatc tgaaataagt tcttctgtaa tctcaaatcc
    15841 ttcatgctta taaacctcga ttgcttgatg attcccctga ttcactcgca aaaagatctc
    15901 tctttccttt gtttgcgcag caagttcttc ataccagcga aaaactgaag aagtcaatcc
    15961 ttgaccacgt actttattta gcaaatataa tttacttaaa taaatttttc catttatctc
    16021 ttcataagca gtgtaaccga cagtcttttc ttcgcttttt aataaaaagt aggacacacc
    16081 ttctgtcagt tctttttgaa tctgtttttt tgactgataa gttttcagca tgtaatccac
    16141 ttgtttttga ccaatgatcg gcacaaaagc ttccggccat acttcttgta tgactgccat
    16201 cagctcatcc agttcatttt ctgttacttt tattttttcc atacacccac gtcctttttt
    16261 acattataaa tgaccttgcg cttgatggaa taaaaagagc atgatcatgc tatacacacc
    16321 caccgaccat ggtttcaaga aaatcacaat ctgcataaat tccctaaaag ataatttcgt
    16381 caaaccagct actagacaga tcaagtcgtc tggcgcaaag ggaagcagca aggtaacaac
    16441 tagtactttt ttaatatctt tcgtttttct tgtcagcaat tcatcaaatt tctgatactt
    16501 ttttggcgat aaaataagct ggacaaaggc tacgccatag taacggacaa gcaaaaatcc
    16561 gattgcttcc ccaatcacta ggccaataca actatataaa agcccaaatc cattgccaaa
    16621 catcagcatg cccgctactg aggagatccc tcccggcaaa ataggaatga tcacttggat
    16681 cacttgcaac agaacaaata tcatcactgc atacttgcca aattgctgga taaacgcttg
    16741 caaagacgtg ctcgatcgaa aaatcccatg atgataagca taaagaatca acacaaagaa
    16801 aaaaaacaga ccaataatgg gcatgatctg aagacatttt tgtaaaactg atcctttttt
    16861 agacatctat attccccaca tcttttttca tttattaaaa ctgcctcctt
//
LOCUS       DAKBVG010000041        16640 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000041 DAKBVG010000000
VERSION     DAKBVG010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.041"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            15..1355
                     /locus_tag="OUD09_000971"
     CDS             15..1355
                     /locus_tag="OUD09_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCT9692254.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVIVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            1573..2016
                     /locus_tag="OUD09_000972"
     CDS             1573..2016
                     /locus_tag="OUD09_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HCT9692255.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILTSKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(2281..3699)
                     /gene="nhaC"
                     /locus_tag="OUD09_000973"
     CDS             complement(2281..3699)
                     /gene="nhaC"
                     /locus_tag="OUD09_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HCT9692256.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPKLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(4102..5325)
                     /locus_tag="OUD09_000974"
     CDS             complement(4102..5325)
                     /locus_tag="OUD09_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HCT9692257.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKEGFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVEQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(5356..6195)
                     /locus_tag="OUD09_000975"
     CDS             complement(5356..6195)
                     /locus_tag="OUD09_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HCT9692258.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETTVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNAALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDEKNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(6212..6892)
                     /locus_tag="OUD09_000976"
     CDS             complement(6212..6892)
                     /locus_tag="OUD09_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT9692259.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(6894..7967)
                     /locus_tag="OUD09_000977"
     CDS             complement(6894..7967)
                     /locus_tag="OUD09_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCT9692260.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IIEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRQKALDYLIKS
                     GVKINILQKNELDSRIKVIEGGR"
     misc_binding    complement(8023..8261)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(8303..8536)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8613..9284)
                     /locus_tag="OUD09_000978"
     CDS             complement(8613..9284)
                     /locus_tag="OUD09_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCT9692261.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYFDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLD
                     KKTEK"
     gene            complement(9371..12049)
                     /gene="mgtA"
                     /locus_tag="OUD09_000979"
     CDS             complement(9371..12049)
                     /gene="mgtA"
                     /locus_tag="OUD09_000979"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HCT9692262.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLTFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASMFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGKFMVYFGPTSSIFDITTYLLMYFVICPAVVDGSFHTLDSSQQVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNVTFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIHRFQELL"
     gene            12708..13145
                     /gene="ndk"
                     /locus_tag="OUD09_000980"
     CDS             12708..13145
                     /gene="ndk"
                     /locus_tag="OUD09_000980"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCT9692263.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAVDAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(13167..14033)
                     /locus_tag="OUD09_000981"
     CDS             complement(13167..14033)
                     /locus_tag="OUD09_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCT9692264.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICSKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLSLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEKKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(14193..16286)
                     /locus_tag="OUD09_000982"
     CDS             complement(14193..16286)
                     /locus_tag="OUD09_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HCT9692265.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWMKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVLYWLDIATFIGILVWLTIKKIPIKSQPVSKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFSESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSAIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWNEFDN
                     AQMQRVPLMIHIPGYTKGSVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSPQHD
                     QVVAFRNGNFVTPKYTILGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKSALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNIAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKDNQ"
BASE COUNT         4800 a         3447 c         2928 g         5465 t
ORIGIN      
        1 gaaagaggtc atttatggaa gataaaaaat tgaaaaaaca acttagctcc agacatatca
       61 ccatgttagc tttaggtgga gcaatcggtg caggtctatt taaaggaagt ggcgaagcga
      121 ttgggatcgc cggcccttct gtattgattg cttttttgat tggcgggctg attttatata
      181 tcgtgatgaa aggtctggga aaaatcgttt taagcggtgg cgacacgcat cacggattat
      241 ctggtttgat acgcccttat ctaggtgctc attctgctga ttttactgat tgggtctact
      301 ggtctatgtg gattatcaat atcatcgctg aagctgttgc agctgcttcc tttttgcagt
      361 tgtggttccc gcacattcca gcctgggtat ttgtttttat gttggctgtt ttaactacga
      421 tcatcaatct ttattcggta cggttattcg cagaaacaga atattggctg gcttttgcta
      481 aaatcagtgt cattatctta ctaatcattt ttgctactta tcttgtcggt cagcaaatgt
      541 tgggcacagg cgttttccct acccttcagc aattgactga ccatggtggg tttactcctc
      601 atggtatgaa gggaatcatc agttctttgc ttgtagttat ctattcttac ggcggttctg
      661 aactgattgc tatcacggta agcgaagcag atgatcctaa acgtgcgatt cctaaagcca
      721 tcaaaggtgt catgggaaga atcatttcct tttacatcat tcctttgttt ttgcttttga
      781 ttctttttcc ttggaatact ttagcaggca cgaatgtcag tccttttgtg attgtatttg
      841 aaaaaatgaa tatcccattt gcagcggata tcgtcaattt tgttattgtc cttgctttgt
      901 tctcatctat caattcgggt gtgtatgcat cttcaagaat tttgttcttc cgtttgaagg
      961 atcgaaaagg ttcctctaga aaactagctg ttttaaacaa gcatcaagta ccgcaacgtg
     1021 ctgttttatt ttgtaccagt gttctttatc ttggtgtagc tctttcttat tttgtcggtg
     1081 acaaattatt tggctatctg gcaggttccc tttcgtatac agtattattg atctggatca
     1141 ttatcagtgc ggcatccttt gtactggcac taagaaaagg aacattatgg gataaaggaa
     1201 tcagcttttt tgcattagct gtcttaggat tgattttcct tggtatcctg ttcacgaact
     1261 ccattggggt aactgtattg actggcctgc tttacctgtt catttatttc agttatcaaa
     1321 aaaagaatga tgcgttcgta ttgacgaatg aatgaaatcg atagaaaatt ttattttatt
     1381 tatgtggaat agatggaact attgaagaaa ggagttgtga caaaagtctt gttgcaactc
     1441 ctttttccga ataaacagtc tcatgacctc ttggtaaacg gtgttcaaaa accctctgtg
     1501 caaaataatt gattgttctc catgctcatg acatgataga agaaaggaat gaaaaaaagg
     1561 agtttgtttt aaatgaaaaa gtattggaag tttttgttga tgatcggtgt ttcaacggta
     1621 gttatgtttg gtatgatgta cttgaatgtc tatgcgctcg atcatatctt cttcagtcgt
     1681 acacgagtat tcatggcgtt gatgatgggt gcagtcatgg cagtcatcat gctgttgttc
     1741 atgtggaaaa tgtatgaaaa caaaaagata aatgttatga tcttagccgc tagtcttctt
     1801 gtttttgccg gttccttgtt tatggtacga agccaaacta ctgtcaacga cacagcttgg
     1861 atgaaggcca tgattcctca tcattctatc gctatcctga caagtaaacg agctgattta
     1921 aaagatcctg aagtaaaaaa attagcggaa gaaatcatcc aggcacaaga agaagagatc
     1981 gacaaaatga aaaaaatgat caaagaaatg gaatgaccat tatccgtcca aataaaaaaa
     2041 gaggatgttc cagaagtgat cagcttcaag aaataagaag aagctacttc tggaacaccg
     2101 tgaataaggg gttgtgaaaa agctgccctg catcaagtaa taagaacagc caaacaaaaa
     2161 tagcttctcc tatttttcgt ttggttaggc ttattaccgc agatgcaagc ttttgaacac
     2221 cgtttattcg taaaagggat tgtgacaaaa aaagtcttgt cacaatccct ttttatgttt
     2281 ttattgttct aattgcgctg tcttgcttac tgcttcttgc ggtttcacaa atttttttga
     2341 gattccccaa cctgtgaagg cactgaccat cgaaaacaca ggagaaagca aactgaaaaa
     2401 tacgaatggc aagtaagtga gtgtaggaat accaaatgta cttgctacga aacttccggc
     2461 aattccccaa ggcactaagt agttgattac tgttccacca tcttctagcg ttctacctaa
     2521 tacagttgga tcaaatcctg ctttttcata agtttcttta aatgcattac caggcagaat
     2581 aacagatagg aattgttcac caataaaaat atttactccg atacttgtca tcaatgttgt
     2641 aaaaatcaat tttggtgtgt tcgtcatctt tcttgatact cgttccatca cggttgagat
     2701 caagccaaat tcgatcaaca atccacctaa tgacaaagtt agaataatca atgacacagt
     2761 aggcatcata ctttcgattc ctccgcgact aagcagtgaa tcgatttgtt ggtttccagt
     2821 atttgaaaca aaccctgatt cgatgacccc agccaacttc gttaaggaag tttgtgggtt
     2881 ttgaataaaa atcattccta ctgctacagc gatattcaac aaaatcgtca aaattgccgg
     2941 cacctttttc catgcacaca gtaacattaa aaataaagga atcagtgacc agattgaaat
     3001 agtaaagttt gcttctagaa catcagtcac ttcacgaata gatgaaagac ttgcggcttt
     3061 acttgtgtgt cctaaaatca tgaataaaaa gaaagaaaca acaaatgccg gaacagttga
     3121 ccacatcata tgcttgatgt gtttgaacaa atcggcatca acaatagctg ctgctagatt
     3181 tgttgattcg gataatggag acatcttatc tccaaaaacg gcacctgaaa caatagctcc
     3241 aacaaccagc gctggattta ggcctagtgt tgtcccaata ccgaaaaatg caatcccaat
     3301 cgttgacata acagtaaagg cactccctac agcgctacct acgattgcac acacaacaaa
     3361 tacagaaggg acaaaccact ggacactgat caatttgaac ccaatgacca ttagagttgg
     3421 aatgatccct gcttgtatcc atacagcaat caatgaacct actaaaataa aaataaagat
     3481 tgggatgatt cctggtttga tgccattgat gattccttcg tgcagccaat ctgtcgggta
     3541 tctttttatc actgcataca acatgaccat tccgatcgct gctaagatcg tgatattagg
     3601 tgagaccttt aatccgacaa cgccaatcgc aatgcctaaa acaataacca ttaatacaag
     3661 tgttgcttct aaaaaacttg gtgttccttt ttttcccatg ataatctctc cttttttagt
     3721 tgttcttatg ttctaaaaaa gttaagatat cggattactc cgccgcaatt catccgctat
     3781 tcctccttta taaaaataaa aagacctgtt ttaaactaag gacgaatgac tccgcggtac
     3841 caccttaatt aaaaacagat ctgtttttca ctttcaaact tttattcaaa tgtatggact
     3901 ttccatgtaa ttcgtcaatt aggcttgatc ggttcacagc gaccaccgac tttctgaaca
     3961 aaacacctaa ccgctactgc gtgaaaagtt gacttgtatg aagtttacct tccataagat
     4021 catttgtcaa tgaaaaagaa aatatttttt catttttctg aaaattattt ttataacttt
     4081 cacacacaag tttatttttt actagaaagt agcaccctct tgtttttttc ttgctactat
     4141 ttcttgatgt tcttgcttga ttctttctaa tagctttggc tctttcaaca gatcaagagc
     4201 tgtattggcg agtagttctg ctgcaatcgc aatagaagca aggcctttgt cacttttagc
     4261 agcagctttg aattcttctg tgtgtccggc aatatatgtc tcagagattg ctaccgtagg
     4321 ctggattgtt ggtatgactt ggctgacatt tcctacatca gaagaaccga gacttgttct
     4381 ttgttccgtt tctatttctt catctggaac acctgcttct tttgcatgct ggaagaataa
     4441 gtcatcaaaa gaaggagtaa cgatgacatc atcaactgca ttttggaaca atccaaactc
     4501 aaaagtcgtg cccgtactta tcgcagcacc tcgtacgata ttttccactt tttcatatac
     4561 tttatctaat gtttgccgat ttcctgcacg taaatagaaa cgtccagcag cgtaatctgg
     4621 aatgacgttg gcagctactc ctccgtctgt aatgatccca tggatattga catcttttgg
     4681 caattgcaag cgtagagcgt tgattccatt aaacacttgg atcaatgctt ccaaagcatt
     4741 tactcctttt tcaggtgctg ctgctgcatg ggaagcgaca ccgtaaaatt tgatgtcgac
     4801 tggatcattc gctaaagatt ctgtcgtttt cccataacga aaagctggat ggacgcataa
     4861 cgctacatcg acatcctcaa aaaaaccttc tcgaacaaag ctgcctttag ctgatccgtt
     4921 ctcgccccct tcttcccctg gggtgccata tacacggatt tctccaccag tttcatcaat
     4981 aaattgctcc actatgctgg cggctaatac ggagtaagta ccaaacaaat tatgcccgca
     5041 tgcatgacca atacctggta aagcatcgaa ttctgccaaa aaggcaattg tgggtccggg
     5101 tttattggaa atataacgtg catcaaaggc tgttcggtgt ccagctactt ctttttctac
     5161 ccaaaaccct tctttttcta attgtttaat caacgctgct gaggaataaa actcatagtt
     5221 acttacttca ggatgttcgt ggatatctag agcaagtgct tgatattctg gtaaacgata
     5281 ttggattaga tccttcaatt ggtttgttga gattgttgtt gtcataaaaa attcctccta
     5341 tgctagtcat ttttcttatt ttctcccgaa tgtttcccat gcaggcacgc ttgatccttt
     5401 tgatgttttc tcgattactt tgactgtgtc ttctgtttga taagcgtcta ctattttttg
     5461 atacgtttta tttttttcat cttcttttct tgcaacgatg atattcacat aaggacgtgc
     5521 atggtcatcc actggttcta agaagatggc atcagaagca ggattaaatc cagcatctac
     5581 agctacaccg ctattgatca ctgaaatgtc tacatctgac aaagcacgtg ctgtttggga
     5641 agcatctaat tcagaaatgt ctagattttt tctattctcg gtaatatcag aaacagtggg
     5701 cgcttgtcct tttgccttat ccactttgat caatccagca gtttgcaata acagaagagc
     5761 tctgccgcca tttgtaacat cattagggat tgctactttt gctccgtctt tgatatcagc
     5821 tacatccttc aaagtgttag aataaattcc cagcggtgca ttgacagtat ttccgatcga
     5881 tactaaatca gttccgtgtt ctttattgta attgtctaaa aagaattgat gttggaaaga
     5941 attcaaatca atcgatccat ctgctaaagc cgcatttggt tgattgtaat ctgtaaattt
     6001 cacgtattcc aaattaatgt tctccttttt caaacggtct ttcacgttgt cccatacatc
     6061 tgtatcttct ccaataattc ctagttttac ggtagtttct tttttgtcag ttgtatctgc
     6121 ttttccttgg ctacaccctg ccaaccaaat aattgctaca aatgtaatga ctacaaaacg
     6181 aataattttt ttcatttttt ctcctccact ttcaatgtgt aattttttta atcagttgat
     6241 tactgataaa ttgacttaaa aaaactaaga ttagaatcaa taatgtagcc acaatcgtta
     6301 catctgtttg gaatcgatta taccctctcg atatagcaag attccctaat ccgcctgcac
     6361 ctactgctcc agccatggta gtcagtccga tcacgttgat gatagtcaac gccgagaccc
     6421 gaatgatacc aggcaaacct tctaacaaat acacacgtaa aataataccg attggactgg
     6481 ttcccatcgc ttctgccgct tcgatcaccc caggatcaac ttctagcagt gcgttctcga
     6541 tttgacgagc aaaaaacgga acaactccaa aaatcaatgg cactaaagct gctttcgcac
     6601 caatcgtcgt tccaactaag aaacgagtag tcaatgctaa aagagaaagc aagataatga
     6661 aagggatgga acgaatgata ttgatcagct tatcaagtat ttcaaatagc agacggttag
     6721 ctagtaatcc attagggcgt gtcactacta acgtcacacc taagatgatt ccgatcatcc
     6781 cgccaatgaa tgctgtccaa aaagacatat atagtgtttg cttcgtcgct tcgacaaatt
     6841 catccggaat caaataaaca ttgggcaaat atgttttaaa gaaatcagtc attttatctt
     6901 cctccttcga tcactttgat acgactatct aattcgtttt tttgtaaaat atttattttt
     6961 acaccagatt tgatcaaata atccaatgct ttttgccgct gtttggtttc tccagctaaa
     7021 gtaacgatca aactccctaa cggcgtttct tgtaataact ctacattgcc atataaaata
     7081 ttcgctgtta cttgatattt gctgtacaga tgagcgatta gaggttcgtt cgtctgattt
     7141 ccaatataac ttaattccac cagccattta ttggcgatct gatcggcaaa agcagaatgg
     7201 gcaatgatcg tttctaatgc ttggtcaaga tgagtagctg ttcgaataaa atctttggtc
     7261 aattcttctt caggattgct gaagatctgg atgctactac ctttttcaat gattcttcca
     7321 tcttccataa ccgctacctt gttgcagatt tctttgacaa cttgcatctc atgagtgatc
     7381 aggacgattg ttaaacctaa ctggcgattc aattttttta acaatgcaag gatctgaagg
     7441 gtcgttttcg gatcaagtgc actggttgct tcatcacata ataatacctt aggttcattc
     7501 gccaaagctc gtgcgattgc tactcgttgt ttttgtcccc ccgacagttg tttaggataa
     7561 gcagatgctt tttcttccaa accgacaagt tccagtagtt cattcacttt ttgacgacgt
     7621 tcctgtttcg attttccaga atactttaaa gaaaaatcaa cgttgtcaaa aatagttcgg
     7681 ctttccatta gattgaaatg ctgaaagatc ataccgatgg cctttctttc ttttcgcaat
     7741 tctttgggag agagctgact caaattcgtc tgattgacaa tgacttctcc tgctgttggt
     7801 ttctgcagca aattcatcac acgaactaac gtacttttcc ctgcacctga gtaaccgacg
     7861 attccataaa catcgccttt ttcaatcgtt aaatctacag aatctactgc ctgtatctct
     7921 ttttcttttt gtttgaaaca tacagaaaca tctcgcaatt caatcattaa aattcctcct
     7981 tatctaaaaa aagcgtcctc aagaaaatga aaatgtcatt ttcttgagga cgcttttttg
     8041 cgtgttacca cctcatgttc aagctgtgtt cacacacaac tcctcttcaa gtacgatcat
     8101 actctagcaa gatatcggtc gcagccgtca tcgccttaca tctatgctcg ggatgaattg
     8161 ctcgatggcc attttccaat agtcgttcct tgcttcttct cagctaccga agctctctgt
     8221 caaaggttct tctatcgtac ttaccatttc aatgcaaata atattcaaaa aaaaatcctt
     8281 ttactaacat aaactatgtc agtaaaagga cgacgtatcg cggtaccacc ttttttcgga
     8341 agtttccttc cctcaagccc aacatctcaa aaagagagat tggcgttcgt tttaacggtc
     8401 gaacagttcc gaatttgcct acttatcgac aaattattct ccaaggccat tttcagcttt
     8461 tcttacttga ctcttctcac cagccagagc ctctctgcaa agtttccaaa cttactttcc
     8521 ttttcaacga aatcgatatt ttgttgttgg gattaatcta catgaggaaa actaaaaagt
     8581 caagcacgaa attcaaatta ttttttctat atttattttt ctgttttttt atctaacatt
     8641 tcaataacag ttcgaagatc ttttccttca gcaagaagtt tcaatttttc cttttcttcg
     8701 acggtataat ttcggacaaa actggaatca ggtatcaaaa caacgcctaa tcctgctcgt
     8761 gaagcagctg ttgctcctac taaagaatct tccactgcaa aggctgaact agcttctaat
     8821 cctgttcgtt ccaaagctgt cagataagga atcggtgaag gcttgttctc cgacacttcg
     8881 tctccgaatg taaacgtgtc aaagtaatca gctaatttaa agtgatccaa gatatctgtt
     8941 gctcttttcg taacagtggt cgtagcaatg cctagtttca tatgacgttc ttttaagtct
     9001 gccaatattt cttttgcata tggttttaag tggatggctc cgcttgtcag ctgttgataa
     9061 aaatattctt cacgcttcgc tcggacttcc tgtacttttt cagggctacc tgctgctttc
     9121 actaaaatat caaaactttg tttccagttt tttccgcccc aagcgtcaac gatttctttt
     9181 gggatatgga tatcaaattg ctgaaatccc caaagccagc catcacgata cgttttttct
     9241 gtgtcaacta acaaaccatc taaatcaaat attgcacctt tcatctatac attaatcctt
     9301 tccaaaacaa tacacatagt ttagcacgac tggtccaaaa ataacagaag caaagaacca
     9361 gccttctcgt ttataagagt tcttggaatc gatggatata gattttcttc acagctgtca
     9421 caagcgccag ataagcgatg actgtagcaa ataaccataa ccagtaattg ccaggaagtg
     9481 gcatcaagcc taattcagca ccaaaacttg taaaaggaag gacagaaccg acaataattc
     9541 ctaatgtagt gataacaaac atggcaaatg ttacgttgct ttggataaat ggtactttcg
     9601 gtgtacgcag tgcatggagc actaaggttt gtgtccatag tgattcgaca aaccatcctg
     9661 catggaataa tgcgatgaaa atcacttgct gtgaactatc caatgtatgg aaacttccgt
     9721 caactacagc aggacaaata acaaaataca tcaacaagta agtagtaata tcaaatatcg
     9781 aacttgttgg gccaaagtaa accatgaatt tcccaatact agatgcattc catttttttg
     9841 gctcgtccaa atattcttta tccatccgat cccatggcaa ggaaatgcag gaaacatcat
     9901 agattagatt caaaaacaga agttgtaaag gcagcattgg taaaaatggc aggaacatac
     9961 ttgcaatcag cacggaaaac atattgccaa aattagaact tgctgtagcc ttgatatatt
    10021 tcatgatatt cccaaaagtt tctcgtccag aaagaaggcc tctttccaaa atcgtcaaat
    10081 ctttttctaa aaggatcacg tctgcagatt ctttcgcaat atccacagct gtatctacag
    10141 aaataccgat atcagccact ttcattgcag gagcgtcatt gataccatct cctaaaaatc
    10201 cgacagtatg accattttta cgtaaagctg tcgtcaaacg tgctttttgc tgaggattca
    10261 attttacgaa tacttcatgg tcttcagcta cttgtgctaa ttcatgatca ctcatttccg
    10321 agattttctc accggtaacc aattcattga tctctagacc tacttgacgg cacactgaac
    10381 gtgtaactaa ttcgttgtcc ccagtcagaa cttttacagc tacaccatgt tctttcagag
    10441 ctttcaacgc ttgtttcgtt gtttcttttg gtggatcaag gaaagtaaga taaccaatca
    10501 aaaccatgtc tgactcatct ttgactgaaa attctccaac gacgcttgga ttggtctttt
    10561 gtgcgacacc aatcactcgt aagccatctt cattaagtga ctgtactttt gcaagtacgg
    10621 cttcttttcg ttcttttgtt aatggtgtaa cagtaccgcc aagatcaata aatttagaaa
    10681 tgttcagcat ttcttcaatc gcgccttttg tgatcatttg tgttttacca gctggatcag
    10741 caaccacaac actcatacga cgacgttcaa aatcaaaagg aatttcatcc actttttgat
    10801 aattgatctt atctgtatct aaagtttctg ttgcttcatc aatgatagcc acatctaaca
    10861 aatttttcag ccctgtttga tagtagctat ttagatacgc atggcgcaaa acacgatcat
    10921 cttcatttcc ttcacaatcc atatgatatt caagaatgat tttatcttgt gtcaatgtcc
    10981 ccgttttatc cgtgcataac acatcgattg caccaaagtt ttggatggca ttcaaatttt
    11041 tgatcactgt tccttttttg gccatcacac ttgctccttt gaccaaattc gtcgtaacga
    11101 tcattggaag catttcaggt gttagaccaa ctgctacaga tagtccaaaa agaaaagctt
    11161 ctagccaatc cccttttgta agcccattga tgacgataac tgctggagcc atcaatgaca
    11221 taaatttgat cagtagcatg gatgttttat ggatgcctaa ttcaaagctt gattgaattg
    11281 gtttttcaga tagcgtttct gctacatgac caaatagtgt ttcatttcca acagaaacaa
    11341 tgattccttc cgcacttcca cttatgacat tacttcccat aaagacaaga ttttcgtaac
    11401 ttgtttcgct ttgctgatcg tcttcacata ctttcgcatg cttttctact ggatagcttt
    11461 cccctgtcaa tgctgcttgg gaaatgaata aatcctttgc ggacaataaa cgcatatccg
    11521 ctggtatcat atcccctgct gaaagccgaa caagatctcc acagacgatt tcttccatag
    11581 gaatctctgt gtactttcca ttacgtttta cagctgccgt tactttcaca agtgatttca
    11641 gtttttcagc cgcttgattg gaacgtacgg actgaatcaa cgtcattgtc ccgctgacga
    11701 tgaccatagt gaagataatg gctacaccga aaagatcttt atctccagga gctgctaata
    11761 catattccgt gatgaatgaa ataattccta atgcgatcaa cactaatgta aatggcgtga
    11821 tgtatgcttt caatatttct acgatgaatg gtgttttttt gccatatgaa atcgcatttt
    11881 caccgtaatt ttctcttgaa cgttcgattt gttcttctgt caatccattt gctgatgcgt
    11941 ggaatagttc aaataattct tgtttcgttt ttccagcaaa agctgcatag attttttgtg
    12001 tatctttctt tttttccatc tcaatctctc ctttgtgtcc aaaagacagc tgttctcgct
    12061 gcttttggtc tttagctatt catcggatcg atagagaaaa aactgaaagg ggctgagaac
    12121 agatagataa tagccttggc ctttcctcta gatccttgtt tttcgaatga ctgtcatcca
    12181 tttgttctca tctcctttac taagtaattt taattcatga cttccaatca tttttcgaat
    12241 agttattttc ccaattagac cacctccttt ttatttttca ccgatgggga tttgcggcaa
    12301 caaaaaaacc tctcgccaaa tactcagcga aaggtttgaa aaagcatatt aaaaaaagct
    12361 tctcagatct catcgttgag ttttgacact gtacaacgta aaaagcagat acctgctttt
    12421 agctacctta tgaaaagcct tatctcgaca attcctgttc tacccattgg cgtctctcga
    12481 cgtttttggg cagtagccta tgtttgtata ggagcctcac ctaacaaatc ttattcggtt
    12541 ttccttcgtt aatataacaa ctaaaaaaga ggatgtcaaa tcaattttcc atgtttttca
    12601 tacttttttt aaaatacaca cgttatattc ctgtctcacg cggttttatt tgtctcattt
    12661 tccacctccc gctataatag aaatatagta ggaaacgagg tgctgttatg gaaagaacac
    12721 ttgttattat caagccagac ggcgtgagac gccatttagt cggaagcatc attcaacgtt
    12781 ttgaagcaaa gggactggcc attgctgaaa tgaagtttga aactatgact cctgaattag
    12841 caaaagaaca ttatcagcac ttgactgagc gcagtttttt tgatgaactg atcgattaca
    12901 tgacttccgg tcctgtcgtt tatcttgtgc tggtcggcga ggaagtgatc gatatcgtcc
    12961 gtaagatggt tggggcaaca aaagctgtcg atgctgttcc aggtacgatc cgaggagact
    13021 acgcgctgcc ggggactgaa aacatcattc acgcttctga ttcccgtgat gctgcggtaa
    13081 aagagatcgc ccgctttttc ccgccattcg atataaacaa accgtctgaa aaagcggcga
    13141 agtgatacaa attctaaact gctggtctag ttgctgggtt cagcaatggc cagcagtttt
    13201 ttctctaagg acctccgttg attctttcct aacatatatt cgagcactgt gaagaattct
    13261 tggtagtctt tcgtcttttt ttcccctaaa gcagcagaaa tcaatctttg ataataatct
    13321 gccatcaatg tttcatagat cactgtactt ctttctttat gtgctaagct ccgtttcata
    13381 tcttcattca cactacgtgc ctttttcaac cgaccatttt ctatataaaa cgaagtcagg
    13441 ttcattaaaa aagtaaagag atactgctca tgattgttca actgtgctaa tcgtcgcatt
    13501 ctttttttga ttttcaaata attgttctca atgaaccgat cactgaatat ccaaggaaaa
    13561 tcagctagaa tagatagttc aaattgacca tagtctggta tctgcatcaa gtattcttca
    13621 atgattcggc attcataacg taaaaattca aattctgtct cagatacttc tgttttttcg
    13681 accatgtgaa gactcaaaac caaataatac agatcttctg ttttcttaaa atgcctacgg
    13741 ttattgctga tctctttata aggatcaaag ctgtcttctt gtacccgatc tagcattcgt
    13801 ctttttttct ttaactttat tggttcatcc agctggtttc gcatccattc atattctgca
    13861 agtgtcatgt tcattcgctc taaaaatgat ctcaatgtta caaatgacat ttttttcact
    13921 gaatttgtct caatcccgac tagagaagtc ttagaacaga ttccttccgc taattgggac
    13981 tgtgtcaact tcttgttgac tcgaagtttc ttgatcaatt caccatcata cataatagtt
    14041 ctccttttta atttaaattt ttccacattc aataaataga tacgttaaaa aggaaaaata
    14101 cacgaaaaaa ataaaaaaat gtttttttca taaaaaatac caaccttcaa gcattagaat
    14161 gttgtctaac tactgaaggt tggtttgatt gtttattgat tatctttttg ctgttcaacg
    14221 tctgttcttg ttactggata taagttggtt gttgttttat tgccattttt tgaaaagaca
    14281 cttgtcgaat gttcgccttt ttgcttttcg atggctatgt tacgttctgc ttcattttta
    14341 tagtcatatt ttttcggatc gatcgtttca aatccgtttg gcacataaaa acgcaagaga
    14401 ttttcttgat tcagcttatc tgaaagactt aaagcagatt tgactttttc ctgatctttg
    14461 gaaatttcct cgtcgattcc tgctgctttc gtatcgatga tttctcctgt ttggttattg
    14521 taagcttttc ctcctaagat ggtgtatttg ggtgtcacga agttcccatt tcgaaaagcc
    14581 actacttgat catgctgagg agataataaa tccgtaccaa aatggagata gtctttatca
    14641 tcgattccca agagatgaag taatgtcggc aaaacatcga tttctccacc gtattcgtga
    14701 ttgactgacc ctttagtata accaggtata tgaatcatta atggcacacg ctgcatttgt
    14761 gcgttgtcaa attcattcca tgtatctggg tcttttccaa gagcacttgc taagtcttta
    14821 ttatctgtat ttgagatgcc aaaatgatcc ccataaatca caaagatcga attttggtaa
    14881 atagcggatt ttttcaggta cgtaaaaaat tgttccaatg actgatccaa ataatgtgcc
    14941 gttcgaacat agttatctac tgtgctattt ccagtgttca aagatggaaa atcgaaattc
    15001 ttatcatccg tataataagg cgtgtgattg gttacggaaa ggaatttcgt gtagaacggc
    15061 tgttgtagat gttcaagata ttgggcagat tcactgaata aatctttatc taatattcca
    15121 taacctagcg tttcatctga ttcatcaaaa taagatcgat caaagaaatt gtcataacct
    15181 aagtttttat atacatgatc tctattccaa aaactagcta cattcccatg gaaaacagcg
    15241 cttgtatagc cttgcttttg tccaaggatc gctggtgcgg cttgaaagac attatctgac
    15301 ccaagttctg tgaataatga gccttgaggt aaaccaaatg tgccagtttc caacatattt
    15361 tcagcatcac ttgtctttcc ctgtcctact tcatgaaaaa aattatcaaa gctgatcgtt
    15421 gcttgattct ggaaaatact atttaaaaac ggtgtgactt cttgtccgtc taccttcata
    15481 ttaatcaaaa actgctggaa actttctaaa tggagaacga tcacattttt ccctttagcg
    15541 atcccaaatg tttcgggatt tggctctgca taatgtttct ttgtataatc taaaacttca
    15601 tcaatcccat tactgcttgc atgcgcgcga acactgctag tcattcctgt tttgattcca
    15661 tcataaatag tatacgcatc gattcctaga tatttgacaa tatatgaacg gtcaaatgtt
    15721 cgctgcagca attgtggacg gttggcttcg ctcagcgcta gatttcctga aaaaataagc
    15781 cctgaaagac aggtcacagc gatagccatc ggtttgctga caggttgact tttgataggt
    15841 attttcttga tcgtcaacca aaccagtatc ccaatgaacg tagcaatatc cagccagtag
    15901 aggacatcat ggggtttcat caaagaaaag gagcttcctg ataacccttg cgacactttc
    15961 gaaaatccca atacagattt gactgtaatg aaatctgtga attcccgata aaaaataatg
    16021 ttcaagtaca acaaaagcgt gtttaaaata tcaatgatca aaagaatggc taatgctggc
    16081 tttggctttt taatatacaa agaaaggcca aaaaacaata aggtagttgc aatcgggttg
    16141 atccacaaga taaagtactg gatcgtacct tccacaccta acgaaaaatc tgcatagtaa
    16201 gcaataatcg tcttcatcca gaaaaagaaa acagctgtca aaacgattgc taaacgattt
    16261 tggcacaatt ttttaagttg tgtcacgtat atcacttctc tctattcaca tttttcctta
    16321 ttttgctcct gaaaaatgaa aattccctaa aaaagttgtt atcttaagat ttttttattc
    16381 tttctaaaga ttattccgtg cacttttcac agcataaaac cgctgtgata taagcgattg
    16441 aaacagtaag gtgataaaag tcttgtcgca gctcctgttt agttgatgca ggacagcttt
    16501 ttcacaactt cttctttaaa cggtgttctc ctgccaactc ctcggccaca agtcacaata
    16561 tttataaaac atgagaagct gttttttgaa attgttcctt gtggctcaga gttaaacggc
    16621 aagctcaaga cctcttgata
//
LOCUS       DAKBVG010000042        16421 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000042 DAKBVG010000000
VERSION     DAKBVG010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.042"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            7..1128
                     /locus_tag="OUD09_000983"
     CDS             7..1128
                     /locus_tag="OUD09_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HCT9692266.1"
                     /translation="MVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRINKVPMPS
                     AGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPKQKSIGI
                     LIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDGLDGLAA
                     GISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLGDTGALF
                     LGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTADKKHLH
                     HRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAILLFELIG
                     LINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            1121..1921
                     /locus_tag="OUD09_000984"
     CDS             1121..1921
                     /locus_tag="OUD09_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCT9692267.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            1921..2748
                     /locus_tag="OUD09_000985"
     CDS             1921..2748
                     /locus_tag="OUD09_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCT9692268.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            2726..3460
                     /locus_tag="OUD09_000986"
     CDS             2726..3460
                     /locus_tag="OUD09_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCT9692269.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            3560..4426
                     /gene="rfbA"
                     /locus_tag="OUD09_000987"
     CDS             3560..4426
                     /gene="rfbA"
                     /locus_tag="OUD09_000987"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCT9692270.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            4440..5012
                     /gene="rfbC"
                     /locus_tag="OUD09_000988"
     CDS             4440..5012
                     /gene="rfbC"
                     /locus_tag="OUD09_000988"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HCT9692271.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            5034..6062
                     /gene="rfbB"
                     /locus_tag="OUD09_000989"
     CDS             5034..6062
                     /gene="rfbB"
                     /locus_tag="OUD09_000989"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCT9692272.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            6160..7011
                     /gene="rfbD"
                     /locus_tag="OUD09_000990"
     CDS             6160..7011
                     /gene="rfbD"
                     /locus_tag="OUD09_000990"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HCT9692273.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            7046..9079
                     /locus_tag="OUD09_000991"
     CDS             7046..9079
                     /locus_tag="OUD09_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9692274.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            9123..10403
                     /locus_tag="OUD09_000992"
     CDS             9123..10403
                     /locus_tag="OUD09_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HCT9692275.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVTEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            10613..11419
                     /locus_tag="OUD09_000993"
     CDS             10613..11419
                     /locus_tag="OUD09_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT9692276.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            11431..12651
                     /locus_tag="OUD09_000994"
     CDS             11431..12651
                     /locus_tag="OUD09_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT9692277.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGKVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            12641..14227
                     /locus_tag="OUD09_000995"
     CDS             12641..14227
                     /locus_tag="OUD09_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCT9692278.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLYINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAQPQNSNYVKVLEVIQQTKQDEIIQQIPFNNFTGENETLSFHVSELA
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKDLPEFFLTDIAG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHYLIENNTRLD
                     QTLKTTINRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSLCIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVLREEVNQAKQLAEGAKAGFELRILCEK
                     ERSFLVEAVAQNGQSWIIEM"
     gene            14266..16404
                     /locus_tag="OUD09_000996"
     CDS             14266..16404
                     /locus_tag="OUD09_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCT9692279.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEVQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALKMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPAEHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVHPGRISGFYEITYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     ILEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPSYIAHDPFYNDNLTKEGIGDFSLRPD"
BASE COUNT         5206 a         2721 c         3498 g         4996 t
ORIGIN      
        1 tttagtatgg ttataagatt ttttctgaca attttattat cactgatatt aacacctgta
       61 ttcaaaatca tttcagtaca aacaggtatg gtcgacaaac caaatgaaag aagaataaat
      121 aaagttccaa tgccttcagc gggcggactg cctatcttta tttcatttgt tatatcgact
      181 ttattcttat tccgtaaaat tatccctcaa tcgtatattg taccgatctt gattgcttct
      241 ttagtaatca tagtcacagg agtgctcgat gataaatatg agctgacacc taaacaaaaa
      301 agtataggga tcttgattgc ttctcttatt atttattttg tagcagatat acgtattgat
      361 tccttcacgc ttccttttat tggatatatc caactaggct ggcttagttt tcccgtaacg
      421 attttttgga tcttcggtat cacgaatgcg gtcaatctga ttgacggttt ggatgggctg
      481 gcggcaggga tttcattgat cggtttaatt acgataggaa tcattggata ttttttcctc
      541 catgcttcta cagtgtatat tccagttgtt attttctgtc ttgtagcaag tattatcggt
      601 ttttttcctt acaattttta tccggcaaag atttatttag gcgatacagg agctctattc
      661 ctagggttta tgatggccgt cctttcacta caagggctga agaacgtgac ttttgtttca
      721 tcgatttctt tgctgatcgt catgggagtg ccggtaacag acacgttttt tgctatcatt
      781 aggagaaaag caaaccgtgt ttctttttct acagcagata aaaaacatct tcatcaccga
      841 ctaatcagtt taggctttac tcataaaggt gctgtcctga cgatttatgc gatggctttg
      901 atgttttctt tcacagctat ggtcatgaat tatactggaa gaatcggaac gatcatattg
      961 attattgcca tgttgtttgc tgcaatttta ttatttgaat tgattggtct gatcaatgag
     1021 aaacatcagt ttattttaaa aacattgcga tttttaggga ataaagagta ccgtacgcaa
     1081 gtaaaacaga aatatttgaa gaaatggaac cgtcatatag atgaataaca aattaaacca
     1141 gccgatcctt attagtattg tgacggcaaa cagtgataaa atttttcgga cactagataa
     1201 ttttatttca actgtaaaag atgaaacagc agtaaaaatc cgaatttttg acaacaattc
     1261 acttcccgag tacaaaaaaa gactaacaga gtataggaaa tatccctaca tcgatatcta
     1321 ttttcatgat gaaaattccg gatttggtta tggacataat cacaatctga tagctagcga
     1381 ttctcgctat ggcattattt gtaacccaga tattttagtg gatgaagaga cagttacagc
     1441 attggtcttg ttattgaaag aacatccgga atgtgcgatg ttagcaccta agattcttaa
     1501 tgaagatgga acaactcagc acctcatccg tgagcagctg gctgtttttg attacgtttt
     1561 gcgctttgtg cctttcaaat gggtcaaaaa aaagtttgac aaacgactgg ctgtgtttga
     1621 atgccgagga ttgcctactg atgctaatag ttatgtacgg atgatctcag gtagttttat
     1681 ggtagctgat attgataaat ttaaagagat aaaggggttt gatgagcgtt tcttcatgta
     1741 ctttgaagac aacgacttgt gtttgagttt tgaaaaagca ggttacaagt tattgtacac
     1801 acccttttat gcagttatcc atttttacgg gaagggtgcg catcgaaatg caaaactgtt
     1861 tcgcgtgttt ttgacttcta tgatgaaatt tttcaataag tgggggtggc gttttttctg
     1921 atgaaaccag aaatattagt agtcgttgtg ctttatcagc aactattcag tcaatctcca
     1981 gcttatggac cattaagcaa agcacttagc gaaaagcaaa ttcagcttat tatttacgat
     2041 aatagtcctc aaaggcagca aaatgagctg tttgaaaaag aaaatatcgt ttatcatcat
     2101 gatccgcaaa atcctggttt agcggtggct tataattttg cgttgtctca agcagatgaa
     2161 ggaacacgtt gtctcgtcac tctggatcag gatacgaggc tagtcgacga ttattttgaa
     2221 ctattgcgta aagtaacctt tacagatgaa tgtgttgctg cagttccgat gattttttct
     2281 gggagccgac aagtttctcc cctatattca gatcgttata tcaatcgtac agctcaagca
     2341 gtagaagttg aaggcccaac agctgaacga atcatggcaa tcaattcagg tgttgcctgg
     2401 tctgtcaaat ttctaaaaaa gattggcggg tttaatcctg ctttttcttt agattttttg
     2461 gatcattggc tgttttggaa agtggatcag ttggaaaaaa ctgtggagat ccttcctgta
     2521 cgaatggaac atgatctttc ggttttagac tatgaaaaag tttcaagtaa tcgataccgt
     2581 tctattttac aagcagaaaa tcgattttat catgaatatg atcaaaggca tttatccagt
     2641 caccgacgac aattattgtt aagaacagct aaacagttct tgaccgtcaa aaaccggcat
     2701 atctggcgga tgactttgcg ctcatatgtt aagaattgga aggtgtgaat tcgtgcgttc
     2761 ggtttgtata gctaccttta atggaggcag atatttaaga gagcagttag atagtatttt
     2821 atctcagctt tcagcagaag atgaagtagt catttctgat gatgggtcag tagatgatac
     2881 atgggatata ttaactgaat acgcagaaaa agataagcga atccagcttt tttcggggcc
     2941 tcgtcaagga ttgatcgcta attttggtta tgcgatccag cagacgaaag gagatctaat
     3001 ctttttggca gaccaagatg atgtttggtt ggagacaaaa gtgaaaaaga tcactgacta
     3061 ttttgagcag aatccggatc agctagtagt agtttctgat ttaatcattg tggacagcga
     3121 gctgcaagag atccatgatt cgtatttttc gtttcgaaat tccaaagaag gatggcttaa
     3181 taatgtgata agaagtcact atattggtgc tggtatggct ttccggtctt ctttaaaacc
     3241 cgatattttg ccgatcccgt cagaagtgcc gatgcatgat atgtggattg gtttgctggg
     3301 aggaaagaag actgggttcc taagagaacc gttaactctg taccgaagac atgatttaaa
     3361 cgcgagtgag attgaaacac attctaaatt acggcaaaaa atttcttggc gtgttcatct
     3421 gattacggcg ttgatcaaac gaaaatggtt taaacgttga taaaagatgt ttaaatcctt
     3481 aatatttcca tgacaaatat aacaattgat gagataataa caaagtgaaa tttgaaatca
     3541 aaaaggagag cagatatcca tgaagggaat cattcttgct gggggcagcg gaacacgtct
     3601 gtacccttta acaaaggcaa catcgaaaca attgatgccg atttatgaca aaccaatgat
     3661 ttattatcca atgtctactc tgatgttggc gggaatcaaa gaaattctga ttatttcaac
     3721 gccacaagat acaccacgtt tcaaggaatt atttggaaac ggaaatgatt taggactgca
     3781 gatcgaatat gcagtacaag aaagtccaga tggattggca caagcgttta tcattggaga
     3841 agaatttatt ggagatgata gtgtctgctt ggtattaggg gacaatattt actatggtgg
     3901 cggtctgtct aaaatgttgc aacgagcagc ttcaaaagat acaggcgcga cagtatttgg
     3961 ctatcatgtc aatgatccag aacgctttgg tgtagtagaa tttgatgaga atatgcaagc
     4021 tttgtcgatt gaagaaaagc ctgctcagcc aaaatcaaat tacgctgtaa ctggccttta
     4081 tttttatgac aatgaggtaa tttctatcgc taaaggtatc aaaccttcag aacgtggaga
     4141 actagagatt acggatgtca ataaagcata tttggataaa ggaaaactat ccgtagaact
     4201 tatgggacgc ggatttgctt ggcttgatac aggaacacat gaatcattgc ttgaagcatc
     4261 tacctttatc gaaacaattg aaaaacgtca gaacttaaaa gtggcttgtt tggaagaaat
     4321 tgcttaccgt atgggctaca tcgataagga acaattgctg gcccttgcac agccattgaa
     4381 aaaaaatcaa tacggtcaat atttattgaa tttggccgct gaataaggag tccttacaga
     4441 tgaaagtaac agatacaaaa ttaacagatg tcaaaatcgt agaaatggac gtctttggcg
     4501 atcatcgcgg tttcttcaca gaaagttatt caaaagcaaa atttgcagaa catggattga
     4561 aatatgactt tgttcaagac aaccattcac tatcagctga agctggtgtc cttcgggggc
     4621 ttcatttcca aaaaggagaa gctgcacaga caaaattgat tcgggtagtc actggagcag
     4681 ttttagatgt agtagtggat atccgcaaag gtagcccgac ttatggtcaa tgggacggat
     4741 ttatcttatc tgaacataat catcgacagt tgcttgtacc taaaggatat gcccatggat
     4801 tcgttacttt gacaccaaat gtgaacttcc tttataagtg tgataattac tataatgcag
     4861 aagcagatgg gggcatcgca tttgatgatc cagatttagc gattgattgg ccaattgatc
     4921 catcaagagc gattatgtcg gaaaaagatc accgtcatcc gacattaaaa gaatttgaag
     4981 cagaaaatcc atttgtttac ggagaaatct aagaattaag gagaaatttt cttatgaaaa
     5041 acatcattgt taccggaggt gccggattca tcggctctaa tttcgttcat tacgtggtaa
     5101 acaatcatcc agaagtgcat gtcactgttt tggataaact gacttatgca ggaaacaaag
     5161 agaacttggc cggacttcct agcgatcgcg tagaattggt cgtaggagat gttgcagatg
     5221 cagaattagt ggaccgtcta gtaaaagaag cggacgcagt tgttcattac gcggcagaat
     5281 cccataacga taattcatta aaagatcctt tcccatttgt acaaacgaat atcattggta
     5341 cgtatacact tttagaagct tgccgcaagt atgatgtacg ctatcatcac gtatctactg
     5401 acgaggttta cggcgatctt ccattacgtg aagacctgcc aggacatggg gaaggcgaag
     5461 gagaaaaatt cactgcggaa acaccatata atccttcaag tccatattct tctacaaaag
     5521 ctggttcaga tctgttagta aaagcatggg tgcgttcttt tggcttacgt gcaaccattt
     5581 caaactgttc aaacaactat ggtccttatc agcatattga aaaatttatt cctcgccaag
     5641 taacgagtat cttaagcggt attcgtccaa aattatatgg cgaaggaaag aatgttcgtg
     5701 actggatcca tacgaatgat cattcttcag cagtgtggct gattttgacg aaaggcagaa
     5761 ttggcgaaac ctatctgatc ggtgcagacg gtgaagaaga taataagacc gtgatggaat
     5821 tgatcttaga aatgatgggc caaccaaaag acgcatacga ccatgtaaac gaccgcgcag
     5881 ggcacgattt gcgttatgca atcgattcga caaaattgcg agaagagcta ggttggcagc
     5941 ctgagtttac gaacttccgt gatgggttgg ctgacacgat caaatggtac gaagaacatg
     6001 aagactggtg gaaaaaagaa aaagaggccg ttgaagcagc atatgccaaa aatggacaat
     6061 aatacagagg aaatttcact gtattaatgg aaaaacacat gtgaggcagg cgctcgcctg
     6121 tctctttttt aaaataaaaa tcaaggagcg gataaccata tgtttttatt aactggagga
     6181 aatggacaat taggtacaga attgcgccat ttattagatg aaaaaaatct gccatatgtt
     6241 tcaacagatg caaaagactt ggatatcaca gatgctgaga aaacaatggc ttatattaca
     6301 gaattaaaac ccgatgttat tttccattgc gcagcttaca cagcagtaga taaagcagaa
     6361 gaagaagcaa aagaattaga tgagaaaatc aatgtcgatg gcacaagaaa tgtagcactt
     6421 gcagcaaaag ctgtgaatgc acaattcgtc tatatcagta cagactatgt tttcgatgga
     6481 aaaaacaaaa atgtagaata cgaagtagac gaccaaacga atccactaaa tgaatatggt
     6541 cgtacgaaat tattaggcga aaaagcagta gaagagattt tagaagacta ttatatcata
     6601 cgaacttctt gggttttcgg gatctatgga cacaatttcg tcttcacgat gcaacgccta
     6661 gcggaaaccc gcgacaaact gactgtagta aatgaccagt ttgggcgacc aacttggaca
     6721 cgtactttgg cagaatttat ggtatatgtc atcgaagaaa aagcaccatt tggtatttat
     6781 catttgtcga acgaaaatag ctgttcttgg tatgagttcg caaaagaaat attgaaagat
     6841 acagatgtag aagttgcacc tgttacgtca gaagaatttc cgcaaaaagc aacacgtccg
     6901 cagtattcag tcatgagcct gaaaaagaca gaagcgttag gttttgtcat accaacttgg
     6961 caagaagcac ttgcgcaaat gctggaagct gccaaaaaaa tgaataagta atttcctttt
     7021 tatcaaatag aatttggagc atgatatgga aaaacaaaca agaagtgcac gacgaaaaga
     7081 aaagccaaaa gcgaatcagg cagattcgaa tttggtgctt gacctgtttc taaaactccg
     7141 ctatcttatt ggcgttttag tcatcgtggt catcgttact tttaatttaa acggaagttc
     7201 acttggtgtt tgggataaat atgtgtcgca aagagacgat gggaaaaaaa ctgatgtgat
     7261 ttttggacag aatcgggaag tgcggtcaga cgaatggctg gtgcagacac ctttttacct
     7321 ctcacaagct gaagaggact tttcgttggt gaacgaggat tattcattaa acggacaaaa
     7381 catgatcatt gcgtataatt ctcctgtaaa ggatattacg gttatcggaa aaccattcaa
     7441 ctggggattc tttttcctcg gaagagatcg aggactttcc ttttattggg cgatgaaatt
     7501 agtcgtcatg gtcttgcttg gattcgaagt actgatgata ttaacgaaga ggaaaaaagc
     7561 tctatcgcta atcggtgcat ttgcgctgac attttctcca gcagtccagt ggtggttcat
     7621 gcaacatgtg ggcgatttga ttttctttac tttgggactg atgattgctt tttatcatta
     7681 tttctacaat catgataaaa aatggctacg aacgttgatg atgcttttgg tggttatttt
     7741 cggtattggg tttattttag tgatttaccc agcccatcaa gtgatgctgg cgtacttgct
     7801 gattttttat tttgttggcc tgcttattta ctatagcaag aaagtttcat gggatctgtt
     7861 ggatgtagtg ttgattggag gggcagtttt atttattggc ggagtcatgg ttcatttctg
     7921 gctaacgtca aaagacgcat tgttagcttc tttgaatacc ttatatccag gaaaacgggt
     7981 aagtactgga ggaaagtgga cgattggcga atttttttct ttccttacaa attggaaaat
     8041 tccttttgaa gatatcaact attcgaataa ctctgaagtg gccctgttct atcacttttt
     8101 tccaactgta ttcctagcaa gtccatttgt gttatttggc aaaaaagata gcgaacaaaa
     8161 gttactggga cgtattttga tgattttttg tctgttttct atcttttgga ttaccgttgg
     8221 gttgccaaaa tggctagcag aaatcactct gctgtcttat gttcctccat taagagccat
     8281 ccttactttt agttttgcgg cttgtttatt atcgatctgg tttatcgctt atatttggag
     8341 agaagatgtt attcctattt ggtataaatt cattttgttg gcagtaaatg catggtctta
     8401 tttctatgcg attacgcatt cgaaaatgga taattatttt tcggattttg aaaccatcgc
     8461 agttgctgtg ttgggaacag tgattttagc ttttgcatta tttggctgga gacgcttgtt
     8521 ttacctcgct tttgtcgcct tagttatcgt atctggcttt ttcgtcaatc cagtcgtaga
     8581 aggtacagga gcgatttttg aaaaaacact ggcaaaagaa atccaaaaaa tcgatcaaca
     8641 agatcctggt aaagtttggt tgtctgaaga tgaattatat aacttcaccc caacattggg
     8701 agtgaaatcc ttcaatacag tacgctttta tccagatata gctgcatgga agaaaatcga
     8761 tccgaaagaa gaatacgaga agtattataa tcggtatgct catacaagag cttttatcgc
     8821 taaggaagat acaaagttca ttttagatcg tccagatatg ttttcggtca cgttgaattt
     8881 tgaagacgca gaaaaattag ggatcgacta tgtgatcagt aaacgaccat taactgatta
     8941 caaccaatta tatgaagcgc agttcacgca gctatatgga ccggataaag acggttatat
     9001 gatttttaaa gtagaatatc cggattatcc agatgtacca caaacacaaa ctcaggaaaa
     9061 tcagctagtc gtacagtaat aggctagctg agaataatcc tgaaatatta gtagaaagga
     9121 gagtgaagat aattcaagca atcgtacaaa aatttgtcaa caaaatgagt gtcggtttct
     9181 tgcttttgtt gctgacgcta gtcttttttg tttggacagc aggggtaggt tttccagcaa
     9241 ctttgtttat atataacaat agttcattac ttatcgcttt tagtgtggct attttgctca
     9301 ttttaaatgt ttataaagca aaaggcgtcg attttcttta tctaggtgtt gcgcttgccc
     9361 ttttcatttt cttttcttac tttcgggaat taagaaatga gtctttattt ggagacttac
     9421 tgtttccttt gattatttta ttttgtttag taattaaatg gatcgagtgg acaaaatggg
     9481 atcgcggtat ttatctgatt gtctttacgg tcttcctcgg aatcacgtta tatcgtgtat
     9541 ttacagagat ccaagtacca gaaggacaaa gcatctgggc tccaggcaat tcgctttctg
     9601 atatttggat caatacgaat acaatcggaa gttctttgat gactcttgct ttattgatca
     9661 gtggatttgc cagttctttt gaacgatggt atgttcgaat catcggtggg atagcagtcg
     9721 ttgcagcctt tttaggtatt tgggtctgcc aatctagagg tgcattagtc gcgttgattg
     9781 tttttgcgat attggatctt ttacctaaat cgttgatgcg tgtcattcgt gcgccattta
     9841 ttgcctacac ggttacgatt cttttagctt tgccgatttc ttatctggcg gctgtctctg
     9901 aaaaagtcaa tttgtttacc ggtcgagaag atatttggca taaattctat cagacgctag
     9961 gagaaaaatc tgaacaagtt ttactaggaa tgaaaacatt tatcttccag cgaggaaacc
    10021 aatttttagg aaaccacaat agttataatt ctattttgaa tatctatgga ttgataggat
    10081 ttgggattgc tgctttgctg cttatccttt ttattggtcg cttaacgttg aaagcagatt
    10141 tatccaacgg gcagatgact tttatctggg ccttttttgc ggtgatgatg cagtcattta
    10201 tggaggatac attgacttca tacccatggg ttccgattat ttatatcctg ttggctatgg
    10261 ccgttcatcg atatgatgaa ccagaagtta cggaggaatt ctatgttatg gaagaggcat
    10321 atgacgatat gacttatgat gatacatctt atgaggaaca accagatagc gctcattctt
    10381 ccaggatcca acgctatcat tgataggcac aaaatacgag agaggctgtg acaaccgtcg
    10441 cagccttttt tggtttattg caattgtcta gcatcttctg taaaatagaa acgttagaga
    10501 aaatcttgta tcatagaaaa ttaagaaaga aataagaacg cagctcggta ccaaaaatat
    10561 ggtaaaatgg cacttgactg taattcactg aaaatcgctt ggaggaacac aaatgtttaa
    10621 agatttgtat ttatttatga aggacattta tcagaacaga aaattactgc tacagttttc
    10681 gatcaatgac ttcaaatcaa gatatgcagg atcgttttta ggaatcttat gggcgtttat
    10741 caatccgcta tttactgtat tgatttattg gctggtattt ggtgttggac tgaaagcaca
    10801 aatgatcgat gcaaaatacc catttatcgt atatttagtt accgggatga ttccctggtt
    10861 cttcttctca gatgcatttg catcaactac ggttgttttt cgggaatata cttatttagt
    10921 aaagaaagtc gtattcaata ttcgtatctt gcccacgacg aaaatcctat ccaatctgta
    10981 cacccattta ttcttcattc tgattggcat agctgtcgcg cttataaatg gtatatatcc
    11041 ttcagcaatg tctttacagc tgatttatta tttattttgc atgtgtgctt tcttgacagg
    11101 attgacttgg ctgacggcat cgatccaacc atttttacct gatatcatgc aattaattac
    11161 gatcgtgctg cagttgatca tgtggacatt accgatatta tggagcccta atcagtttga
    11221 ttcaggtctt gtaagcatat tgaaattaaa tccgctgtac tatgttgttc aaggttatcg
    11281 tgagtcattt ttaagtgaag cttggttctg ggagcatgga acatatactt tgtatttctg
    11341 ggttttcacg ttagtaatgt tcgtaatcgg ctctgcagtg ttccgtagat tgaaaccaca
    11401 tttttcagat gtgctgtaag gagaatagct atgacagaat atgcaattag actaaaagat
    11461 atcacaaaat cttataatat gtacgcaaaa ccttcggatc gatttcgtga agccctcaat
    11521 ccttttaaga aatcctatca tgatgtattt tatgcgttga aaaacgtgaa tgtagaaata
    11581 aaaaaaggtg agatgatcgg tttcgtggga gaaaacggat cgggaaaatc tacaatgttg
    11641 aaaatcatta cgggagtact gacaccaaca tccggcacga tggaaatcga aggaaaaatc
    11701 tcagctttgc tagaactagg atcaggattc aatcctgagt attctggcta tgaaaatatc
    11761 tatttaaatg gaatggttct aggattttca agagaagaag tcgatgaaat ggtcgatgat
    11821 atcattgaat ttgctgacat cggcgaccat atttatcaac ctgtaaaaac ctattcaagt
    11881 gggatgttcg ttcgtctagc ttttgctgta gcgatcaatg tgaatcctga tattttgatt
    11941 gtggatgaag cattggcagt aggagatctt gaatttcaac taaaatgtat ggaaaaattt
    12001 acagaaatca agaactcggg aaagacgatt ttattcgttt ctcatgatat tagttcgatt
    12061 cgccgttttt gtgatcgttc ctattggttg aaaaatggaa aagttgtcga atatggtgat
    12121 acgatggaag taacgacgaa ttacgaaaac tttttgaaga aaaaatcagt taaaacagta
    12181 gatcgaaata aaaaccaagt agaacgcttc gcagctccag atatcgtaga cgtgacgaaa
    12241 gccgaattgt tggataaaga taaaaatcca gtggatatgc tggaacaagg agaaaaacta
    12301 tacgttaaag taacctatga agtaaaagac gatacaatca aaaatccagt tttaggaatt
    12361 gctctgcgta cagtcgacaa tttttatgtg tgcggactca atactctact agatggtatc
    12421 aagatccctt ggaaaaaagg tgagaatatc ttttatctgg agtatgaaaa gttaggtttg
    12481 ttagctggtg aatattattt tgacgttgcg gtctttgaag aaaatgcaac ggttcctttg
    12541 gtatacaaga caaaatacat gaacttgttt gtcagcggtt cttatatcgg agaaggcatc
    12601 gtggtcttag accataaatg ggaagaaggg actcatagca atgaaatatg attttgaaat
    12661 ggacctagat gaacaaagca gtgtgggaaa aattgccgca cagatcaaac caggaagtaa
    12721 agttttagag tttggtcctg gaaacggccg attaacgaaa catttgatcg gagcaaaaca
    12781 atgtgaagtg agcattgtcg agctagataa ggaattgttc gattttgtca gcgagtttgc
    12841 ccaagatgga ttttatggag atatcgaaag ttttgaatgg gcaaactatt atgctggaca
    12901 aacatttgat tacgttttgt ttgccgatgt tttagaacat ttggtagatc ctggaaaaac
    12961 gttgaagaaa gttagagaat ttttgaatga agaaggagaa attctaatta cattccctaa
    13021 ccttgcgcat aattctgtca tgattgattt gttcaataat caattgccat gggcttctta
    13081 tggtttgcta gatgaaaccc ataattcttt ttatactcat gatggcttcc aaaaagtttt
    13141 tgaaaaagca ggattataca tcaatattga agattatttg tacttagctg taggtgatac
    13201 agaattaaaa tcaacttatg aagaattgcc ggaagctgta cgttatgatt tcaaaatgcg
    13261 accatttggc gaagtctacc aatacttctt ctcattgatg aaacatccag tagcacaaag
    13321 ttccattgcg cagccgcaaa attctaatta tgtgaaagta ttagaagtaa tccagcaaac
    13381 gaaacaagat gaaataattc aacaaatacc attcaataat ttcacagggg aaaatgagac
    13441 actaagtttc catgtatcag aattagcaga acgaatggtg tttcgcttcg cgcagcaacc
    13501 tagctttatc gaattcagcg cagaagctgc tggagaaaaa ctcacattta tcgattcaaa
    13561 tgctgtagtc aaaacagtaa atgattgtta tttgtttgat ggtaaagatt taccagagtt
    13621 ctttttaacg gatattgcag ggaaagaagt gactatccat tgtcactatc gttttatcgg
    13681 agaattgaca ccaacaatga aagaattgct ggaaacaatc cgtccaatgg ctgaagttac
    13741 aaagcagctt tctgagaaaa atgatgaatt agaaagagaa aatcattatc ttattgagaa
    13801 taatacaaga ttagatcaga cgttgaaaac aaccataaat cgctattgca cgttgttgga
    13861 aagtgatgaa tggacaatca aacatagatt agggcgacgt aagaaagaaa catcgaaaaa
    13921 aatccaagaa aaagagcttt ctctatgtat tgacgaaaaa atctgggatg cagaaacaaa
    13981 gatattgaaa atcatcgggt ggggaatcgc taattcggat cgccaaccat tgtcctataa
    14041 gttatcagcc gaccaatcac cattttttga agcaattcct gttttacgag aagaagtcaa
    14101 tcaagcaaaa cagctagctg aaggagcaaa agcaggattt gaactgcgga tcctttgcga
    14161 gaaggaacgt tcattcttag ttgaagcagt ggctcaaaat ggtcaatcat ggatcattga
    14221 aatgtaaata atgattaact tataaagata aggagtaaac gaatcgtgca aaaaaaagaa
    14281 attgccgtat ttatcgatca aatcactcgt gaacgggcca ctggcgattt gcttattgtg
    14341 ggctgggcaa ttgatgaagt gaccaaagaa atccctacga taaaggtaga aaaagaaaat
    14401 gtgattgctg aagcaacaca tgtcgtaaga ttggatatca atcatttata taatcttgat
    14461 gttaagaccc aaagcggttt taaggtacgt ttatcaggga aaatgagagg aaaagcaatc
    14521 ttagacttcc aaacagcaag acatcaaaat ggcatagctg tgaaattaaa cggtaaatat
    14581 ccttatgatg acggcattga atcttcatgg gaaagaaaaa aaagattact gaaaaaaggg
    14641 ataaattatg caaggactca tggcgtaaaa aaagcaatcc gccgggtcaa actggaattg
    14701 aaaccaggat cgatcgacta tgcagagtgg atcagtcgtc atgagaaacc tgatctgaaa
    14761 gagcaaagga aagaagtaca aggatttgcc taccgcccat tgatttccat acttatgcct
    14821 gtctataacg ttgaaatcaa atggctcgaa aaatgcattg attcggtact tgatcaaacg
    14881 tatgatcact gggaactatg tatcagtgat gatgcttcta ctgatccagc tatccgaaag
    14941 tgtctagaat cctatcaagc aaaagatgat agaattaaag tcgttttccg acaagaaaat
    15001 ggacacatca gtctggcaac taattctgca ttgaaaatgg cagaaggaga gtttatagcg
    15061 ctcttagata atgatgacga attacctcca tttgccttat atgaagtggc aaaagtgcta
    15121 aacgtacatc cagaactgga tttgatctac agtgacgaag ataaaatcga tgcagatggg
    15181 aatcgctttg acccgcactt caaagcagac tggtctcccg atacattgat gggaaataat
    15241 tatatctctc acttaggcgt atatcggaca agcattgtta aggaacttgg cggtttccgt
    15301 aaaggatatg aaggctctca agattatgat ttggttcttc gtgtgacaga acaaattcct
    15361 gcagaacaca tttaccacat tgaccgtgtc ttgtatcatt ggcggacgat cccgggatca
    15421 actgcaagca atggagaagc taaaagctac atctatgatt ctggagtgaa agcattgaca
    15481 gatgctctat ctagaagaaa tatcaaggga agcgtacatc ctggcagaat ttctggtttt
    15541 tatgaaatca cttatgatgt tttgcaagaa gacttggtca gtgtgatcat tccaactaag
    15601 aatggctatg aagatctaaa aacttgtgtg gattcgatca tcgaaaaaac gtcttatcca
    15661 aattatgaaa tcattattgc agataatgga agtacagatc ctaaaatgca agaattattt
    15721 gcagaataca agcatcagct gaaagaccga tttatcgtcg aattaattga cattccattc
    15781 aattattcac gtatcaacaa tctggcagca gaaaaagcaa acggcaaata ctttttattc
    15841 ttgaataacg atacagaagt gattgaacct gactggatga cagcgatggt ttcttatgca
    15901 cagtttgatc gaatcggctg tgtaggagca aaattatttt atccggatga tacaacgcag
    15961 catgcaggtg tattattagg gatcggtggc gttgcaggac atgcattgaa taactatgat
    16021 cgaacacatt gtggttactt tggccgattg gtcattgacg tcaattactt agcagtcact
    16081 gcagcttgta tgatggtcaa ggcagctgac ttcaatgctg tcaatggatt tgatgaaata
    16141 ttagaagtag cattcaacga tgttgacttg tgcttgaaag tttatgaact gggtcgttac
    16201 aatgtctatg cacatcaagc agaattgtac catttcgaat ctaaatcaag aggttatgaa
    16261 gatacaccag aaaaacaaag acgttttgct ggggaaatca aaaaaatgca agataagtgg
    16321 ccttcttata ttgctcatga cccattttat aacgataatc taactaaaga aggaatcggc
    16381 gatttttcgt tgagacctga ttgattaaaa gaactgtatt t
//
LOCUS       DAKBVG010000043        16374 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000043 DAKBVG010000000
VERSION     DAKBVG010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.043"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            2..550
                     /gene="hslV"
                     /locus_tag="OUD09_000997"
     CDS             2..550
                     /gene="hslV"
                     /locus_tag="OUD09_000997"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HCT9692280.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            564..1964
                     /gene="hslU"
                     /locus_tag="OUD09_000998"
     CDS             564..1964
                     /gene="hslU"
                     /locus_tag="OUD09_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HCT9692281.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALIKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            1982..2773
                     /gene="codY"
                     /locus_tag="OUD09_000999"
     CDS             1982..2773
                     /gene="codY"
                     /locus_tag="OUD09_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HCT9692282.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            2855..3730
                     /locus_tag="OUD09_001000"
     CDS             2855..3730
                     /locus_tag="OUD09_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HCT9692283.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNREIFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADAVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(3862..4500)
                     /gene="plsY"
                     /locus_tag="OUD09_001001"
     CDS             complement(3862..4500)
                     /gene="plsY"
                     /locus_tag="OUD09_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCT9692284.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            4627..5061
                     /locus_tag="OUD09_001002"
     CDS             4627..5061
                     /locus_tag="OUD09_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCT9692285.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEVADDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            5227..7272
                     /gene="parE"
                     /locus_tag="OUD09_001003"
     CDS             5227..7272
                     /gene="parE"
                     /locus_tag="OUD09_001003"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCT9692286.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLTVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEVPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            7284..9734
                     /gene="parC"
                     /locus_tag="OUD09_001004"
     CDS             7284..9734
                     /gene="parC"
                     /locus_tag="OUD09_001004"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCT9692287.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     VTIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            10011..12242
                     /gene="pflB"
                     /locus_tag="OUD09_001005"
     CDS             10011..12242
                     /gene="pflB"
                     /locus_tag="OUD09_001005"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCT9692288.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            12486..13247
                     /gene="pflA"
                     /locus_tag="OUD09_001006"
     CDS             12486..13247
                     /gene="pflA"
                     /locus_tag="OUD09_001006"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HCT9692289.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGNKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            13332..14258
                     /locus_tag="OUD09_001007"
     CDS             13332..14258
                     /locus_tag="OUD09_001007"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HCT9692290.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            14440..15336
                     /locus_tag="OUD09_001008"
     CDS             14440..15336
                     /locus_tag="OUD09_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HCT9692291.1"
                     /translation="MNYYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKK
                     EFSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLS
                     VDEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRATAGNEKYRFITLSSIEHF
                     PLTLANYFFVQKNQLPVTKAFKELAELIGDVNEAYFFDQIEVIVDRIRKNKYKNRRPS
                     IFHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEETAFLKRQIFSELS
                     KKFIPHAFSYIKEYRTVLVSKT"
     gene            15470..15595
                     /locus_tag="OUD09_001009"
     CDS             15470..15595
                     /locus_tag="OUD09_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCT9692292.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYSILWEELAA"
     gene            complement(15729..16361)
                     /locus_tag="OUD09_001010"
     CDS             complement(15729..16361)
                     /locus_tag="OUD09_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9692293.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
BASE COUNT         5814 a         2783 c         3497 g         4280 t
ORIGIN      
        1 catggtcgaa tcaccatttc attcaaccac tatctgtgcc gtcgaaaaag acgggaaatt
       61 tgctatggct ggagatggcc aagtaacaat gggtgaatct gttgtcatga aaggcactgc
      121 aaagaaagta cgacgtatct acaatggaga agttgttgta ggtttcgccg gaagtgttgc
      181 agatgcgttt actttagaag aaaaatttga aggcaaacta aacgaatata acgggaatct
      241 acaacgcgca gctgtcgaat tagctcaaga atggcggaca cagcaatcta tgcaaaaact
      301 tgaagcgatg ctgatcgtaa tgaatgataa agaaatgctt cttgtttcag gtacaggaga
      361 agttatcaca ccagatgatg gtatcttagc tatcggatca ggagggaatt ttgcgttggc
      421 tgcagcccga gcaatgaaaa actatgctca aaacgacatg agtgcgaaag aaattgcaga
      481 aaatgcgtta aatattgccg cagatatctg tatctttact aatcataata ttatcgtaga
      541 agaactgtaa gaggtgagta gatatgtatg aattaaaaca aacaccaaga caaatcgtaa
      601 gtgaattaga tgaatatatc gtaggacagc acgcagcaaa aaaatctgtt gccgttgctt
      661 tgcgtaaccg ctatcgccgt ttgcagttag atgaaaaaat gcaacaagat attacaccaa
      721 aaaatatgct gatgatcgga cctacagggg tcggtaaaac agaaatcgca cgacgtttgg
      781 caaaaatcgt caatgcacca tttgtcaaag tcgaagcaac aaaatttact gaagtcggtt
      841 atgtcggaag agatgtagaa tcaatggttc gggatttagt agaaaatgcg atccaaatcg
      901 tagaaaaaga gcagtacagc cgtgtataca caagtgctaa aaaaaatgca gaaaaacgtt
      961 tggtaaaaat cttggtacct ggtatcaaaa aagaacagaa acaatccact aatcaatttg
     1021 aacaaatgat gaatcttttt aatgctagtc agcaaaatac cgctgaaccg caagaagaag
     1081 tgacagaaga tattcgaatc aatcgccgta cagtcttgga acaactacaa aagggtttac
     1141 tggatagtcg tgaagtaacc attgaagttg aagaccaaaa gaaacctgca ccaatgatga
     1201 acaatggatt ggagcaaatg gggatcgacc taaatgaaac tctaggtgct ttgacaccga
     1261 agaaaaaagt ggaacggacg gtaacagtca aagaagcttt agagttattg atcaaggaag
     1321 aatcagcaaa attagtcaag gatgctgata tccatagtga ggcaatcaaa ttagcggaag
     1381 aaagtggaat tatttttatc gatgaaatcg ataagatcac ttctaaatca cagcaatctg
     1441 gagaagtatc cagagaaggc gtccaaagag acatattgcc aatcgtcgaa ggttcacaag
     1501 taaatacaaa atacgggatc atccaaacag atcatgtctt attcattgct tcaggagcgt
     1561 tccatttatc taaaccgagt gatttgattc cagaactcca agggcgtttc ccaatacggg
     1621 tagaattaga tgatttgaca gctgaagatt ttgtgaaaat tctgactgaa ccgaataatg
     1681 ctttaataaa acaatatgta gcattgattg gtacagaaaa tgtgtcagtt acgtttacaa
     1741 aagaagcaat tgaacggatc gctcaaatcg ctttcgatgt caaccgtgac acagataata
     1801 tcggcgcacg cagactgcat accattttgg aaaaactact agaagatcta ttatttgaag
     1861 caccagatat gcaaatggga gaaataacga tcacagaagc atatgtggat gacaaacttg
     1921 gctcaattgc ggcaaatgaa gatttaagtc gctatatttt ataaaaaatc ggaggacgac
     1981 aatgacagac ttgctaacga aaacccgcaa gataaacaca cttttgcagc aaaagaatac
     2041 ttttgacatg caagcagatt tgccttacga taaaatggct gtcacactag gtggtattct
     2101 tgacagcaac acctatatca taggaagtga cggagtatta ctaggttata atgaacgtca
     2161 cgatgtgaac aatgaacgaa tcaaaacaat gtttgttgag aaacaatttc ctgaatctta
     2221 tactgataca gtggcacagt tgatgaaaac tgaagctaat atcccaatta ctagtgatat
     2281 tactgcattt ccggtagagt caagagagaa atatccattt ggtctgacga ctgtcgttcc
     2341 gatctttgga gctggcgaac gtttgggtac gattatctta tctcgtatgg aacaatcgtt
     2401 tgcagatgac gatctagtct tagcagaata tggagctacg gttgtcggta tgcagatcct
     2461 ttatcagaag tctcgaagca tcgaagcaga tgtacggagt gcaacggctg tccaaatggc
     2521 aatcaacaca ttatcttata gcgaattgaa ggctgtacaa gctatctttg aagcattgga
     2581 tggagatgaa ggtcgtctga ctgcttctaa tattgccgat agtatcggca tcactcgttc
     2641 tgtgatcgtc aatgctttac gaaaattgga atcagctgga atcattgaat ctcgttcgtt
     2701 aggcatgaag ggaacttatt taaaagtatt gaattcccgt tttaaagacg agttagcaaa
     2761 acacagttat tagaagtaag gggactgggg cagatagaaa tgtttgttcc agccttccca
     2821 ttttaaaaag taagtcgaag ttggaggaat ggcaatgact gttattattc aaaatgacca
     2881 gctgatcgcc gaaatcgctg aacacggtgc ggaattgatc agtctgaaat caaaagaaca
     2941 tggtttagag tatatctggc aaggtgatcc agcgtattgg ggcagacatg caccggtttt
     3001 attccctttt gttggacgtt taaaagatga tcagtatgtt tatcaaggga aaacatatca
     3061 aatggggcaa cacggatttg ctcgggatat ggattttgaa gtcattgaac atggacaaga
     3121 aatggctagt ttcatcttaa ggagtacgcc ggagacatta gaaaagtatc catttgattt
     3181 tgaattagtg atcagctatg aattaggagg agacggcatc acggttcatt atcaagtggc
     3241 aaataccggt gaaaaagaaa tgttcttttc tatcggtgga catcctgctt tcaatgtacc
     3301 tcttgaagaa ggattgtcat ttgaagattt ctatctggca ttttctccat tgaaatcaag
     3361 attacgccta ccattgaaag gcccattcat cgatatggat caaaagacgt taggtcagac
     3421 aaatacgaat ctggctttga atcgtgaaat ttttgagcaa gatgccttgg tttttgaaac
     3481 aaggggtcta aatgcgtatg ccattcgaag tgaaaaatct tcccatagcg taactttaag
     3541 ctataaagat atgccttatg tagggatctg gtctccttat ccaaaagaag cgcctttcgt
     3601 ttgtatcgaa ccgtggtgcg gaatcgctga cgcggtgaac agtagtggaa acttgatgga
     3661 aaaagtaggg atcaatcaac taggtgccca tgaaatcttc aagacaaaat attccatcac
     3721 agtgaagtaa gaggttgtga ccgagaagtt agcagggaac accgtttacc aaggtgtgta
     3781 aaaaagctat cctgtaagta ataagggagt gtgacatcga tttgtcacac tcccgtttta
     3841 tctctttgga atgaaaaagt atcaggattt tttcttgctg ctattcaaac caaacgggat
     3901 tcgactttca gtatggtttc tgatacgctt gatattgtct ttatgccgat aaaagataaa
     3961 ggaagaaact aaaaaggcaa tgatcgtcag taaccaattg ggcttagcca aaatcacagg
     4021 agctacataa ggaaggacga tcgtagataa agtgatcaat actgcactga tcatactgct
     4081 caaactcacc atacttgtac aaaataaact aacgataaag atgatacaag aataaacaaa
     4141 aaatacagga ttaaatccta aaagcattcc tgcacttgtc gcaacagctt ttcctccttt
     4201 gaacccagca aaaatcggaa atgtatggcc aagaatggcg cataccccaa accaaagagg
     4261 gttaatccca gaaaggtgga agatcatcgg caaacaagta gccaatgtac cttttaaaat
     4321 atccataagc aggacaacaa tcccggctgt tttgcctaat acacgaaatg tattggtcgt
     4381 tccagtatta ccgctcccgt gttgtcggat atcttttttg aaaaacaatt tgccaatcca
     4441 gacaccggaa ggaatagaac ctaaaagata tgctaaaata agtaataaga tcaatttcat
     4501 tttttaccct ctttcaagtt gaaaactatc ctttatttta gcatgaatag gtaacaggaa
     4561 caattgaaaa aatgaaagat acaagtataa tagatggaat aaaaagaaaa gcaaggggtg
     4621 gcagaaatgt ctgtaaaaaa tccaacacaa gaaacgattt tcaactattt ggaagaagca
     4681 aaaaatattg ccgtcgtagg cttgagcaat aaaccagaac gaacaagtta catggttgca
     4741 caggtattgc aagacaatgg atataaaatc ataccagtaa atccattatt ggctggtgaa
     4801 gaaattctag gcgagaaagt ctatgcgaat ttgacagaaa tcgatgatcc aatcgatatc
     4861 gtagatgtat ttagacggag tgaattttta cctgaagtag cagatgattt tttgaaaact
     4921 gatgctaaag tgttctgggc acaattaggg attgaaaaca aagaagcagc tgagatgcta
     4981 agaaaagcag gacgtacaga tatcgtgatg gatcgttgta ttaagattga acttggtaag
     5041 ttgaaagaaa accaaaaata aaaatgacca caaagaagag atgatcgata gaggggaatg
     5101 tcgaaaaagc ggttaggaga taattaaaat tgatttctcg tctaccctct tttttttcac
     5161 gaccttttct agtatgataa tcgtggtgcg gttttgccgc aaagtattat tgaaggagcg
     5221 tttgccttgg caaaaaaagt aaacaacgaa tacaatgact cgtccatcca agtattggaa
     5281 ggattagagg cagttagaaa aagaccaggg atgtacattg gatccactga cagccgaggc
     5341 ttgcatcatt tagtctatga aatcgtcgat aatgcagttg acgaagcatt atcaggttat
     5401 ggcagtgaga ttgatgtgac catccatgaa gacaatagta taactgttgc agatagcgga
     5461 cgtgggatgc ctgtaggaat gcatgcttcc ggaataccaa cagttgaagt gatttttact
     5521 gtgctgcatg ctggcggaaa gtttggtcaa ggagggtaca aaacttctgg tggactgcac
     5581 ggtgtaggag ccagtgtggt aaatgcctta tcgaaatggc taactgtaac gatcgttaga
     5641 gatggcgtag agtatcagca gaaatttaaa aacggcggta aacctgacgg tacgttgaaa
     5701 aagataggaa aaacaaaaaa agcaaacggg acgactgtcc attttttacc agacgataca
     5761 atattcagta caacgaaatt ttcatatgaa attttagccg aacggttaag agaatctgcc
     5821 tttcttctaa aaggcgttaa aatctctctt tctgatcttc gtggagaagt accagtcaag
     5881 gaaatttttc attacgaaga aggaatcaaa gaatttgttg attatctgaa tgaagaaaaa
     5941 gatacattga caccagttgt ttatttttcg ggagaaaaag aaggtatcga agtagaggtc
     6001 gcttatcaat acaatgacgg gtattcagaa aatgtcttat cttttgtcaa taacgttcgg
     6061 acaaaagatg ggggaaccca tgaagctgga atgaaagcgg caatgacgaa atcttataat
     6121 gaatatgcga gaaaagttgg tttgctgaaa gaacgggaca agaatctgga aggtagtgat
     6181 ttcagagaag ggttagctgc tgtactttct atccgtgtac ctgaaaatct tctgcaattt
     6241 gaagggcaaa caaaagaaaa acttggtacg ccagttgctc gtaccgtagt agataacgtc
     6301 atcagtgaac aaatgggctt ttatttgcag gaaaatagcg aaatgagcca gatgttggtc
     6361 cgtaaagcaa tcaaagccag agaagcgaga gaagcagcac gtaaagccag agaagaaagc
     6421 cgaaatggga agaagcgtaa aaaaggcgaa tctcttcttt caggaaaatt gacacccgca
     6481 caatcccgaa accctaagaa aaatgaattg tatttagtcg aaggggattc tgctggcgga
     6541 tctgctaaac aaggaagaga cagaaagttc caagcaattc tacctttacg aggaaaagtc
     6601 atcaataccg aaaaagctaa aatgcaagat atcttgaaaa atgaagaaat caatacgatg
     6661 atctatacga ttggtgcagg agtcggtcct gagttttcta tcgaagactg taattacgac
     6721 aaagtaatca tcatgaccga tgcggataca gacggcgccc atattcaagt attactgctt
     6781 actttctttt atcgttatat gaaaccgctg attgaagctg ggaaagtata tatcgcgtta
     6841 ccgcctttat ataaagtatc caaaggacaa ggaaagaagc aagtcattga atatgcgtgg
     6901 acagatgacg aattggcagc aatgattaaa aaagtcggca aaggatatat gctgcagcga
     6961 tataaaggac ttggagagat gaatgcggaa caattgtggg aaacaacgat ggatccgact
     7021 tcccgtacgt tgatccgcgt ccgtatcgat gatgctgcgc aagctgaacg tcgagtaacc
     7081 acactgatgg gtgacaaagt agaacctcga agaaaatgga tcgaaaatca cgtgcaattt
     7141 acgctcgaag aagatggcag tatcttagat aaaaaagagg atactgaaat ctctccttca
     7201 gtttctaatg atttattaga tgaagaacga gcagataaaa atgaaaacaa tcagttattt
     7261 gaagtagaat aggaggtcac ctgatggaaa atcgacaaga agtacaagaa ttaactttag
     7321 aagaagtgat gggtgaccgc ttcggacgtt attccaaata tattatccaa gaacgtgcac
     7381 tgcctgatat ccgtgatgga ttgaagccag tgcaacgccg gatattattt gccatgaaca
     7441 aagatggcaa tacgtatgac aaaggattcc gtaaatcagc aaaatctgtc ggaaatatca
     7501 tggggaatta tcatcctcat ggtgacatca gtatttatga agcgatggtc cgcatgagcc
     7561 aagactggaa gttgcgggaa gtactgatcg aaatgcacgg gaataacggg agtatggacg
     7621 gtgatccacc tgctgcgatg cgttatactg aagccagatt atccaagtta agtggagaga
     7681 tgctagcaga tatcgaaaaa gaaacagtcg atctcgtgtg gaattttgat gatacagaaa
     7741 aagaaccaac agtccttcca gctcgatacc caaaccttct cgtcaatgga tcgacaggta
     7801 tatctgctgg atatgcaaca gaaatcccga ctcataactt agctgaagtg attgatggca
     7861 caatctatat gatcgatcat ccacaagcga gccttgaaaa attgatggaa tatattcctg
     7921 gacctgactt tccaacagga gggatcttgc aaggaaaaga tgaaatcaaa aaagcttatg
     7981 aaacagggcg aggaaaagtt attcttcgtt cgaaaacaaa gatagaggca atgaagggta
     8041 ataaacagca aatcgtgatc acagaaatcc catatgaagt aaataaagcg accttggtga
     8101 aaaaaatgga tgagatccgt ttgaataaga aaatcgatgg aatagctgaa gtacgagatg
     8161 aaagtgatag aacaggtctg caaattgtcg tcgaattgaa aaaagacgcc aatgctcaag
     8221 gaattttgaa ttatttgttc aaaaacactg agctacagat caattacaat ttcaatatgg
     8281 tcgctatcga ccatatgact ccgcatcaag ttggactgaa agacatttta agaagttata
     8341 tcgagcatcg aaagcaagtc ataacaaaac gtagtcaatt cgatttagcc aaagctcaaa
     8401 aaagacagca tatcgtagaa ggattgatga aagctttatc gattctggat gaagtaatcg
     8461 taacgattcg tgaaagtaaa gacaaaaaag acgccaaaaa gaatttagtc gacgtctttc
     8521 aatttacaga agaacaagca gaagcaattg tcacattgca attataccgt ttgacgaaca
     8581 cggatatcac agagcttcaa aaggaatccg aaagcttgat tgcgcaaatc acagagttga
     8641 ataaaatcct ttcaaatgac aaagagcttt tctctgttat gaaaaaagaa ttgcgagaag
     8701 tgaaaaaaaa ttattcttcc gctcgtctaa cgacaatcga agacgaaatc gaagaaatca
     8761 aaattgatac gcaagtctta gtggcacaag aagacgtaat tgtcagtgtg acgcgagaag
     8821 gctatgtgaa gcgaacaagc cttcgttctt atagtgcttc caaaccagaa gaaatcggga
     8881 tgcgagaagg cgactacttg ctatactcag gtgaattgag tacattggac catgttcttc
     8941 tgatcacaaa taaagcaaat gtgatctacc gccctgtcca tgaactaccc gatttgaaat
     9001 ggaaagatgc tggtgagcac atttctcaaa caatctccaa tctgtctgta gacgaatcga
     9061 ttttagctgt tttcccatat caaaaaattc aagcagagaa aacatttgta ttcatcagca
     9121 agaatggatt gatcaagcaa acacgaatga cagagttcga accatggcgc acctataaga
     9181 gccgtccgct cagctgtatg aagctaaagg cggaagaaga tgaactgatt gctgtgtatc
     9241 tgaaagaaga tcagccaggc ttagatgttt tcttagtaac gcatcaagga atgggtttgc
     9301 gctatccttt aacagatgtt cccgttgttg gagcaaaggc agctggagtg aagtctatga
     9361 atttgaaaga tggagatagg attatcaacg ggctgcttgt gttagcagaa ggtgatacgc
     9421 cgatcgtaat cgttactcag cgaggagctg ttaaacgcat gcttgctcaa gaaatcagcc
     9481 aaacttcacg tgcgaagcgg ggagtgacgg tcatgcgtga gctgaaaaaa aatccgcacc
     9541 gtattatcta tatgtcggaa ggaagatcaa aagaaataac cttgatgaat caaaaagggc
     9601 agcagttaac gattgaccca actgatttcc ctataggtga tcgaacatcg aatggatcat
     9661 ttgcgatgga tgagaaaaaa ggtggagaag tcatcgaagt tatcgatgct cctgagattt
     9721 tcatcgagtc gtaactgttg tagtatttta atgaagagac gccatctgta ttaatacagg
     9781 cggcgtctct ttttcattca tttttaataa atccttatac gtttgcaaaa aaagatgaaa
     9841 gtgttcacaa tgcttaaatg ttggtatgtc aacgttgatt taaatatatg taaataaaac
     9901 ataaaaattg ttaaataaaa aacaagaaaa cccttgcaaa gatagggtcc tgtgttatac
     9961 taatcacgta aaaactgtta cataaaacag aaatttagga ggtccataca atggagcaat
    10021 ggaatggatt taaaggtacc aaatggaaag aaggcgtaga cactagagac tttattcaaa
    10081 ataactacac tgaatatcgc ggtgatgaca gctttttgga aggcccaaca gaagcaacag
    10141 aaaaattatg ggatgaattg caaaagttaa atgatatcca attcgagcgt aacggtgtct
    10201 atgatatgga cacagaaatc gtatcaacta ttacttcaca cgatgcaggc tatttggata
    10261 aagatttgga aaaaatcgtc ggtctacaga cagacaaacc tttgaaacaa gcattcatgc
    10321 cgttcggcgg aatcaatatg gctaacaatg cgctaaaaag taatggctat acaccaaacg
    10381 atgaattgac acatattttc actgactggc gcaaaacaca taatcaaggt gtatttgacg
    10441 cttatactgc tgaaatgcgt gctgctcgta aaaacaaaat cattacaggg cttccagacg
    10501 cttacggtcg cggacgtatc atcggtgact atcgtcgtgt ggcattgtat ggaatcgact
    10561 atttgatgga acaaaagaaa aaagatcatg atatgactgg ttatcataca atgagtgaag
    10621 aagtgattcg tttacgtgaa gaaatcactg aacaatatcg tgcattagcg caaatgaaag
    10681 aaatggctgc gaaatatggc tatgatattt ctaaaccagc tgcaaatgca aaagaagctg
    10741 tccaatggct atacttcgga tatttagcag caatcaaatc acaaaatggt gctgcgatgt
    10801 caatcggacg tatctcagca ttccttgata tctatatcca acgtgatttg gataatggag
    10861 taatcacaga aaaagaagca caagaattga tcgaccactt agtaatgaaa ttacgtatgg
    10921 tcaaattcgc gcgtacacct gaatataacc aattattctc aggattccct atctgggcta
    10981 ctttatccat tgcaggtatg ggattagatg gtcgttcatt agttacaaag aatgatttcc
    11041 gtctgcttca tacattaaca aatatgggac cttcaccaga accaaacttg actgttttgt
    11101 attctgaaca ccttccagaa ggattcagaa catatgctag cagaatcgct atcgaaagtt
    11161 cttcgattca attcgaaaat gataatcagc ttcgtgaaca atggggttct gatgattgtg
    11221 cgattgcttg ctgtgtatca ggaacagttg tcggaaaaga catgcaattc tttggtgctc
    11281 gtgctaactt ggctaaaaca ctattatatg ccatcaatgg cggagtcgat gaagtgactg
    11341 gcgcccaagt tggaccagaa tatcgtccaa tcacatcaga aatactagac tttgaagaag
    11401 tgaaacataa cttcatgaat atgatggatt ggttagcaga actatatgtc aatacgttga
    11461 acatcattca ttatatgcat gataaatatt cttatgaagc agctcagctg gctttgatgg
    11521 attctgactt gcaacgcaca tttgctacag gtattgctgg gatttctcat gcggcagaca
    11581 gcttgtcagc gatcaaatat gcaaaagtaa aagcaatccg taatgaaaaa ggaatagcag
    11641 ttgattttga agtggatggc gacttcccta aatacggaaa tgatgatgac cgtgcagatg
    11701 aattagtttc atggatcttg gaatacttca tgggacaaat caaacgtcat aaaacttacc
    11761 gcaactcaaa accaacaact tcattattga cgattacttc aaatgttgtt tacggtaaaa
    11821 atacaggaaa tactcctgat ggccgtgaag ctggaaaacc acttgcgcca ggagcaaatc
    11881 caagttacaa tgcggaacaa agtgggttat tagctagcct gaactcaaca gctaaaatga
    11941 attacggctg tgcattggat ggaatttcaa atacccaaac aatcaatcct tcaggtcttg
    12001 gaaaagaaca agatacaaga atcgaaaact tgcgtaatgt cttagatgga tacttctcaa
    12061 aaggagctta tcatttgaat gtcaatgtat tcagtaatga tttattgcgt gatgcaatgg
    12121 aaaatccaat gaaatatccg aacttgacga tccgggtatc tggttatgca gttaaattcc
    12181 gtgacttgac aagagaacaa caattagatg tcttgatgag aacttcacac gacagaatgt
    12241 aaaaagttcg tgaaaaaagt ttttcgactt agcgtcgaaa ggacgttgaa tagccatcgc
    12301 atcaaaatcg aataatcagt atgcttattt gaaagaaacg atttacaggg gcaggacttg
    12361 tatgttccgt ccctgtaaat tttacacata acgaagcggc aattgcgaaa aattttgttc
    12421 agaaagcttc gcttcgacag ctaacttgta cttaaaatga aatcaaagaa aagatgtgaa
    12481 cttaaatgga agaaaaaaca atcggctatg ttcattctat cgaaactttc ggttcagtag
    12541 acggacctgg actgcgcttt gtcgtgttta tgcaaggatg ccgcatgcgt tgccaatttt
    12601 gtcacaatcc agatacttgg aatattggcg gagggaaaga atacacagca gatgaacttt
    12661 tagataaagc agaacgcttc agaccttact ggggaaataa aggtggtatt actgttagtg
    12721 gtggagaacc tctattgcag atcgattttt tgatcgaact atttaaaaaa gcaaaagagc
    12781 ggaagatgca tactacattg gatacatgcg gaaaaccttt tacgtatgaa gaacctttct
    12841 tttcacgttt tcaagaattg atgaagtata cagatttact gttatttgac atcaaacaca
    12901 tcgataatga agagcataaa aaactaacgc attgggacaa tgaaaatatt ttagaaatgg
    12961 ctcaatattt gtcaaaaatc aacaaaccgg tttggattcg ccatgtacta gttcctgagc
    13021 gaagtgatta tgatgaatat ttgatccgac tagacaactt tattcaaacc ttgtcaaatg
    13081 tagacagagt agagattctt ccttatcata cgatgggaaa atacaagtgg gaaacacttg
    13141 gtttgaaata tccattagaa gggatcgaac cacctacaaa agaacgtgtc gaaaatgcta
    13201 aacgattgct tcatacagaa gagtatacaa aatatttaac gcgataggtc gcatcggaaa
    13261 gaagataagt attgcttctt tccgtttttt tgctattatt aaataggaga aatcacaaag
    13321 gagcgaatga catgtcaaaa attttagttt ttggtcacca aaatccagat acagatgcaa
    13381 tcggggcagc cattgcgttt tcacacttgc aaaatgaatt aggtaaagaa acagaagcag
    13441 ttgctctagg tacacctaat gaagaaacac aatacgcatt agaccacttt cagctatctg
    13501 caccacgagt agtagagtca gctaaagcag aaaatgcaga tgtcatgctt gtggatcata
    13561 atgaattcca acaaagtatt tcagatatca gcgaagtaaa tattttagct gtagtagacc
    13621 accatcgtat tgcaaacttt gaaacagcta atccattgta ctatcgtgca gaaccagttg
    13681 gatgtacaag tacgattatt ttaaaactat ataaagaaaa ccaagtaaca attcctaaag
    13741 aaatcgcagg aatcatgctc tcagctatca tttcagatac attgttgttc aaatcaccaa
    13801 catgtacgcc tgaagatgaa gcagctgcaa aagaacttgc agcaatcgct gaagtcgatc
    13861 taaacactta tggacttgaa atgttgaaag caggaacaaa tctaagtgat aaatcagctg
    13921 cggttcttct tgatttggat gcaaaaagtt tcccaatggg cagttccaac gttcgtatcg
    13981 ctcaagtcaa tacagtggat cttaatgaag tgatggatcg acaagcagag ctagaagcag
    14041 caatggaaga tgaaatgaaa gcaaatggct acgatctgtt cgtattagtt gtcacaaaca
    14101 tcttagacag tgattcagaa ttacttgttg ctggtaaagg aaaagaaaaa gtagaagaag
    14161 ctttccaaac aactttgacc aacaaccgtg ctttgcttaa aggtgtcgtt tcacgtaaaa
    14221 aacaagtcgt acctcaattg acggctgtat ttagctaatc aaagggtagt atgaagcatt
    14281 caaaatgaat ggataagcga ttgatataaa aagaaacata gaacatacta aaggccgaag
    14341 aaatggagga gaataacagt caattatgac tcttgttctt cctccttttt acgtaaaagc
    14401 cttttaagta aaataaaacg gatagaaaag agaacatata tgaactatta ttattcaaaa
    14461 aataaggaaa acttttatca aaaactgacc gatgatccgc ttttttcttc attgaccgat
    14521 tatctatatg aacatcgaga acaagaaacg attctaagag aattgaaaaa agaattttcg
    14581 caaaataaat tttcccattt tcttgatcta ttgatcgatg caggattaat caagagggaa
    14641 gaacgaagat atcatttgaa ctttccaata tttgatccta aagattactt gcaacaagcc
    14701 acttcggctg ctgaaaccat agctgaacaa ttgaagagac tctctgttga tgaacaaaaa
    14761 ttagcgatgg gagaagttat ttgggcgtat tgtttcgaag atgaacggaa agaagcgtat
    14821 ttttatggag taagaaacag ccgagagaca gaattgctga gggcaactgc aggaaatgag
    14881 aagtaccgtt ttatcacact ttcctctata gaacacttcc cgcttacttt ggctaattac
    14941 ttttttgtac agaagaatca gcttcctgta acgaaggcat tcaaagaatt agccgaactt
    15001 attggagacg taaatgaagc gtactttttt gatcaaatag aagttatcgt tgacaggata
    15061 cgtaagaata aatataaaaa tcgccggcct agtattttcc atcagtctct acttgtgacg
    15121 aatactataa aggaagaaga atcattcaca ttagaattac ctattgtcga aaaaaacaat
    15181 tttgagattg aattaccaac attggatcct tcattgacaa tggaggaaac ggcctttctt
    15241 aaacgacaaa tcttttctga gctgagcaag aaatttatac cgcatgcatt ttcttatatc
    15301 aaagaatata gaacagtttt agtgagtaaa acctaagaga gcagtaagca aaaattatac
    15361 tgactcttgc aggaggagcc tttctcttcc tttatggtta aaagaattat ttgagtattt
    15421 tgcctatcta ccacctattg gattcactac atggtctgga aacaaaggaa tgcaaaaaaa
    15481 tggtgagaaa tgtggaatga caaaggaagt agtgataaga aaagttagat ttttaaataa
    15541 tcaatattat gattcagtca agtatagcat tttatgggaa gaactagctg cttagattag
    15601 gaagtccatt tgatgatcca gaatcactaa gaaacgaatg gcgagtcatt aataatttat
    15661 ccaatcttgg gttattcttt tagattgaag tgtttaaaac taaaaaagca tttatgaagt
    15721 cgtaatagtc aggcttcaaa atgcttttta ttttgtttgt tttttctttg ccacataaat
    15781 acacctagac tgacgatgaa gagcatatcc aacagatttc ctgcgacttg gatcgtcgaa
    15841 gggttggtca gtggcattaa agcaaggaca aaaaaggaaa acaagctgta aataaaacag
    15901 gtgacaggaa ctaatcgaaa gaagagtgga catttcaatt ggctaaaaat gagtgaaaaa
    15961 ataaaataag taaaaatgga taaaatcacg actaaaccat tcatgataaa gaagaaaagc
    16021 gtattgtgat taaacgaata aaaaagaatt gctaaaaaag aaaacgtttt aaatgcaatc
    16081 aatacgcttg aagcttcgtt taacgtacca ggatgtcgat gaaaaaaata ggcaccgaaa
    16141 taaacagaaa acagtaagcc gcaaagagaa gacaaaatca aaaaaaacgg attttcttcg
    16201 gcttctgtga tatcagaatg atccaaaagc aaccagccta atgcactaga aggcgcatgt
    16261 gaggtcttaa ggtatgaata acatagaaaa aataagatcg tcaaagttgc aaaaaaagag
    16321 cctaaatgta aaaaattagt ggttctacgt tgaatttcca taatatctcc aaat
//
LOCUS       DAKBVG010000044        16347 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000044 DAKBVG010000000
VERSION     DAKBVG010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16347
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.044"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(95..493)
                     /locus_tag="OUD09_001011"
     CDS             complement(95..493)
                     /locus_tag="OUD09_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HCT9692294.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(490..1263)
                     /locus_tag="OUD09_001012"
     CDS             complement(490..1263)
                     /locus_tag="OUD09_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HCT9692295.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1260..1943)
                     /locus_tag="OUD09_001013"
     CDS             complement(1260..1943)
                     /locus_tag="OUD09_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HCT9692296.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2110..2997)
                     /locus_tag="OUD09_001014"
     CDS             complement(2110..2997)
                     /locus_tag="OUD09_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCT9692297.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(2994..3863)
                     /locus_tag="OUD09_001015"
     CDS             complement(2994..3863)
                     /locus_tag="OUD09_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCT9692298.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4000..5286)
                     /locus_tag="OUD09_001016"
     CDS             complement(4000..5286)
                     /locus_tag="OUD09_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCT9692299.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5559..6137
                     /locus_tag="OUD09_001017"
     CDS             5559..6137
                     /locus_tag="OUD09_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCT9692300.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6137..7066
                     /locus_tag="OUD09_001018"
     CDS             6137..7066
                     /locus_tag="OUD09_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCT9692301.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7063..8799
                     /locus_tag="OUD09_001019"
     CDS             7063..8799
                     /locus_tag="OUD09_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HCT9692302.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8862..9698)
                     /gene="pheA"
                     /locus_tag="OUD09_001020"
     CDS             complement(8862..9698)
                     /gene="pheA"
                     /locus_tag="OUD09_001020"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HCT9692303.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9695..10204)
                     /locus_tag="OUD09_001021"
     CDS             complement(9695..10204)
                     /locus_tag="OUD09_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HCT9692304.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10197..11492)
                     /gene="aroA"
                     /locus_tag="OUD09_001022"
     CDS             complement(10197..11492)
                     /gene="aroA"
                     /locus_tag="OUD09_001022"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCT9692305.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMSADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11505..12596)
                     /locus_tag="OUD09_001023"
     CDS             complement(11505..12596)
                     /locus_tag="OUD09_001023"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HCT9692306.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12613..13779)
                     /gene="aroC"
                     /locus_tag="OUD09_001024"
     CDS             complement(12613..13779)
                     /gene="aroC"
                     /locus_tag="OUD09_001024"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCT9692307.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13781..14845)
                     /gene="aroB"
                     /locus_tag="OUD09_001025"
     CDS             complement(13781..14845)
                     /gene="aroB"
                     /locus_tag="OUD09_001025"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCT9692308.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14878..15900)
                     /gene="aroF"
                     /locus_tag="OUD09_001026"
     CDS             complement(14878..15900)
                     /gene="aroF"
                     /locus_tag="OUD09_001026"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HCT9692309.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
BASE COUNT         4713 a         3619 c         2906 g         5109 t
ORIGIN      
        1 ttcataagta ttcacgagaa aaatacaaac aaaaaagcgg aaataacgct catccgcctt
       61 cttgtttgtg tttatttcct cttcaaacgc ttgtctatga ttcagacttt tctctatcgt
      121 tattttcatc taaaagttct tgcattccga taatttctcg atattgatga atcttagtga
      181 taccaatgac tttgtctaca tcaatactaa aacaaatacc attacctgga atatcgatgt
      241 tgatgccttg accaatcgca tccattactt tattcgtcaa actatcgggt actaatgtca
      301 acacaatatc tttttctggc gaaatgtcca aacctaaaaa tttccctgaa tcatgtacac
      361 cagaacctct tccatgtaag atcgttccgc ctgctgcacc tgcttctttt gaaaagtcga
      421 taacatcttc gcccattcct ccgtcaacga tcgtcacaat catgtgaaga ttcaatgcta
      481 caagatcttt catttgcttt cccccaattt ttgcaatctt tctttttcga ttctgtcttt
      541 ttcatctttt cgtttataaa tatatcctag tactaaagtg gataatattg gtgtcaatgc
      601 aatcattgca accacaccaa acccgtcaat gatcggatct cttccttcaa tttgtgcagc
      661 gattgccacg ctcattgata aaataaatgt cgaacacatc gggcccgttg ctactccacc
      721 gttatcaaaa gccattgcta aaaagatctt gtccactttt cgtcctaaaa taaatgctag
      781 cccgtatcct gggatcaaga aataataaag agagaatccg acgagcaaac gccacataga
      841 caatcctact gctgcgccga tcccaattga aattaccatc agcatgattt tcgatcggat
      901 tctcccctcg ctcaattctt caacctgttt gatcattaca tgaatcgccg gctcagcaaa
      961 ccctactaag aaccccatta aaaagccaag gggaaccagt atataattgt aaggcaatcc
     1021 cgcaatagca ctgcctaaat gctgtcctac cggtacataa gccatattga ctccgtgcag
     1081 gaaaaggatc aacccaatag tcgttatcaa gaaacctttc ataatgtcaa agaaacgctt
     1141 tttgcgcata cgaaaactaa tgatattcat taaaacaaat aggattacga taggtgccac
     1201 tgccaaaatg acttccatgg taacgatttc gattccatct atcacttgct gccagaaact
     1261 cattggaaga tcacccccaa aatcatcacg gcaagaatcg gccctaacga agcaataccg
     1321 accattccaa aactgtcatt ttcatttttc ttttgtttga tcatactagt catccctccg
     1381 gcaagggcca agataaatgg aacagttact ggaccagtag tcactccccc cgaatcaaaa
     1441 gcaatcggca taaattcgga actggtgaaa aatgaggcga tcaaaacgcc tatgtagcct
     1501 atagccatca attgataata ggatagcttg aatacgaccc gaagcagcgc aaaggctaga
     1561 aatactccag tgccgacgct gacgatccca atcagcaaca ctctgccaat ctctccgtca
     1621 gatatatcat atacttgttg tcccaaaact tgcacggaag gttcagcaat cgtgatcacg
     1681 atcccgatag caaatcccaa actgaccaaa acggtcaaac ttttcttttt gatcatatat
     1741 ttccctacta gatctccgac ttccatcata gaataatctg ctccaaacaa aaagagcgtc
     1801 attccgatca tcatgattgc cgcaccaacc agaaatgaag cgaggtcttc tccttctaac
     1861 ggtgcaaaaa caaacgttaa aatgacaatc aggacagtca tcggcaatat agcagcaatc
     1921 acttctttaa aattttttag catgtatcct cctcctttac tctttaagtt taccttaaaa
     1981 tgaaagagtt ttcgagcaca aacttaaaac gttatcattt aatttttctc aacataaaat
     2041 aacacacgtc ccaaaagtat ctttttactt ggaaaaatga ctttgaagga acgcgcttat
     2101 tgtgtttttt tattttacag agccgatcat tccttgaacg aattgctttt gcaaaacaaa
     2161 aaatacgatg gcagttggta aagtagcgat gactaatcca gccatgatca ttccatagtc
     2221 tggtgtatag gaagcaccca tagcagataa aacaagcgga accgtccgtt tatcaggaga
     2281 ttgcaaagct actaagggcc ataaataatt gttccagcta ctcatgaatg tgatgattgc
     2341 agccgcggca taggtatttt tagccgtcgg catatacact ctaaaaaaaa tacttagttc
     2401 ttttagaccg tctaatcgag cggcttctac tagatctttt ggaaaagatt taacattttg
     2461 tcgaaagaag aagatcaaaa aagcagtgct aacagatggc aggatgacaa ccaagaaact
     2521 gttgattccc aaaaccgttc cgttcaattg tgaaaacatt cgatataaag ggatcatcaa
     2581 tgccgcaaaa ggaaccatca tcgatagcag tagaatattg aaaattaggt cttttcgttt
     2641 actttgataa atctcaaatc catatcctgc cattgacgaa atcagcaatg caaacacagt
     2701 ggtcaccaaa gcgatcatcg ctgaattcca aagggaacga gtaaaattca gctcattgga
     2761 aaacaaattt tgaaaattga tgatcaagtt actgccaatc tttatttttc ccgatgtgat
     2821 atccacgggt gtattcgtca ttcccaaaac catccagaca aaagggaaaa tggaaataaa
     2881 tgataggatg cttaatgctg tatactttaa tactgaccca attgtcgaac gaagttctaa
     2941 tgcttgtttt ttgacgttgg ggcgttcgat tgtttctttt tctaccatat ttttcatcga
     3001 ctttctcctc ctactttcgt ttggattatc gacaatatca caataaagga aacaataaca
     3061 aaggaaactg ctgccgcata tccaaaatta ggcgtgaatt tataactcaa attatagatg
     3121 tattgagata gggtggtcgt tgcatttgct ggtcccccgc cagtaatatt ttgtacttca
     3181 tcaaaaagct gcaatgtccc gatcgtcgaa gtaatggaag taaacaaaat gattggtttt
     3241 aaatttggga tcgtcacttt gataaactgc tgccatttag aagcaccgtc aatagatgct
     3301 gcttcataaa tctccgaatc aatattttgc atgcctgaaa ggaaaaaaat catattgtat
     3361 ccagtccaac gccaagtgat cgaaagaatg atcagaactc tcgcccaaat tggatgagtc
     3421 aaccagccaa tcggtgtgtc aatcatacca atgcccaaca acaagttgtt tacaaggcca
     3481 gattgagaaa atagactttt catgatcaga gaataactga ccatcgaagt gatacatgga
     3541 agaaagatag cggttcgaaa caacccttta tatttcaact ttttatcatt taaaaggttg
     3601 gaaataaaaa gtgctagaaa caacatgatc ggtacttgta caagtaaata caggaatgta
     3661 ttaaaaaaag cttttttaaa agttgcatct gtcaacatcc gctgataatt acctaacccg
     3721 ttaaatgtca tattgacagg gggaccactt tgaaaagacg tgatcaaagc caaaaccatc
     3781 ggaataaaaa ccatgattga tatcaaaatg actggcaaga ttaaaaaagc atgcccgttt
     3841 ttgaataaat ctttctgctt cattcttttc cctccaattc tatcattgat tatctacatg
     3901 aaaagacaag aaagacaata tctgtaaggc agacgtgatc ttttttgtct tttcgtttgt
     3961 ctgatgaaaa agcgaagaaa ttcacttttt taccaccgat caattagcca attgtgcttc
     4021 tacttgtttt tgtgtatctg ctagtacctt gtctgtctct tctccattga tgattcgatg
     4081 aagagcttca gctacgactg cttcaattgc ataggtctca tgtccgtaat tcacttctgg
     4141 tatttcagat gtccatgttg aaaaatcgtc aaagatcttt tgaccactgt aaaattcaga
     4201 agcttcttga taagcttctt gttcattcgc gctaagcatc gtagatacta gtccgatttc
     4261 tttcgcaaga gtgcccatca gttcttcatt cgaagcaaac gttgcttcta ggaaatcttt
     4321 cgctgaatct tctccagcta ctcctttgat cacataccag ccagcgccgc caaggttaga
     4381 agcttgcgct ttttgcagat tttctggaag tgcaggtatt ggtgcaatct tccattttcc
     4441 agattggttt tctgctcctt ggatcgttga agaataccaa gcgccagttg ggctagaagc
     4501 tactgctcct gattgtatcg cagttactcc agcattccag tcagaggttt gttcgactag
     4561 atcttctttc aaaagagtgg caaacaattc aagaccatac tttaaagatg cattgtcttg
     4621 gatcgtgact gtctccccat cttcagatgt ataccattct cctgcctgtt gcataatcat
     4681 ccttactcga ccaaggtcag aaggattgac ttcagcgatt ttctttcccg tcttttcttt
     4741 gacatctcgc gctacttgaa tatactcgtc ccacttcaga ttctccatat cctcttctgt
     4801 ataacctgct tctgccatca gatccgtacg atagaagttt gctgttacgc ccgaatcgaa
     4861 aggaacaccg tatattttat ctcctacttt gtttaccgca aatttatacg gtgcaaaatg
     4921 atcttccttt acaatagcag acaaattagc gaatgcatct ggataagaag tcaaatagcc
     4981 ttgtatccta taatcttcaa tcaagacgat attgggcaat ccttctgtat tcccgctcgc
     5041 taaagcggtg ttcagtttct gaacgatatc atcctgagac atcgtaacaa cttctatctc
     5101 tgtttcttcg ttctcataaa ctttttttgc ttcattcact gctttgatat tgaatgtctc
     5161 atcccacgcc cagacagtga ctttttcttc tttgtctgct gtactactag cacttccgca
     5221 tcctgttaag aaacctgatg ctgccgctaa acaaacgact ccagcaatta actttctgat
     5281 gttcattttt atccgctcct tttccatcca ttagttgttt acgtttgcta cattcatcat
     5341 accaaaattt gaaaacggtt tctttattat tcagatactt caaaaatttc tcctaaaata
     5401 ataaaaaaat acaaatcaac tatgacaaaa ggaataatct tagcatctag accaaagatc
     5461 gaaatggtga ggtttcttta aaataaaaat atccttgcat tttctcctag aaataagtat
     5521 ttttaaatta caagactatt cttaaggcgg gtaaaaaaat gccaatacgc gtaattctta
     5581 tcgatgatga acagacgatt ttagatggaa tgaaacagtt gatcaattgg tctgagcacg
     5641 ggtttcaatt aatagggact ttttccagtc cgctgaaagc gcttgatttt tctttggtac
     5701 atgcaatcga tatcgtggtc acagatgtaa tgatgcctga attaaatgga atcgaattat
     5761 cgcggctatt aaaacagcaa caacctgaaa cacagatcct tatcctctct agctatgatg
     5821 aatttgatat ggttaaagat tcttttaaag aaggcgtcgc tgattatctt ttaaagcctc
     5881 gattgagtcc tgattttttt ctaaattcct taactgccgt ttcacaaaaa atcaaaaaaa
     5941 agcccaacaa aagcccaatc accaatcgtt atgagcagat ctgcgagaat ctttcacagc
     6001 agattgctgg acaaactcgt ccattgcttg accctcttct ttttgaacac accttgtttt
     6061 tctgttatat accgaaaaac ggccattcag ttcaaaacaa ggctctaaac aaaaacagtc
     6121 gatcacccat tcattgatgg atagcgacga ttcgtttact atttatcctg tttctacctt
     6181 ttcggaagaa tccggctatg taatcaatac tgattccaaa gatacactta ggaagtttgt
     6241 tcaaaagttt cgtcaagaag tttttccagg tgattttttt gttgtttctg attcttttgc
     6301 aatccaagat atcaatgaaa tattccaaga gatcagaaat tgcaccaaag gacaacgttt
     6361 ctatcataag ggaaagaacc ttgcttatca gcatgaactt cttccgttat taaccaacca
     6421 cgagcagcaa gcaagtagtt atttaacaaa aatcgttcat aaagactacg ctttagctgt
     6481 caaagaactt gagaatttct tcacgacgat attggaacag cctattgatc cagcttattt
     6541 gaaacatgag gcaatcaatc gtttctatgc attactcaac gctttggctg acgagcatgt
     6601 tgggaatgaa gaactttctt tgctcaagat cgcccttcct tctttattag ccgatgctga
     6661 gtatttcgaa gatttttcta ttcttgtcaa agaaacactt gagaaactca cccacttttc
     6721 tgcaaatgca aaaaaagaca atttcgatat cttgactttg atctacgagt tcgtccagga
     6781 aaattatgag aaagaaatca atttacaact tttatccgaa aaatatcatt tttcatatag
     6841 ctatctctca gccatcttta ctgaaaaatt tggtatcaac ttttcaaaat atctaaaaaa
     6901 agtccgtatc agtaaagcca agctcttttt gacggaaaca cggcttactt tgacggaaat
     6961 ctgtgagaaa gtcggctata ccgaactggg ttatttctcc agagttttta aagaagagac
     7021 aggaatgaca ccttctcaat accgtaaagg aacttttgta aaatgagaaa aaaaatcaaa
     7081 gagcatgaac tctttgccaa acttctgttc attttgttca tcggtctttt tctgcaagcc
     7141 atcgtcattt ctttgtttat ttatcatcga tcaagggatg cctatatcca gctgttcaat
     7201 caatcgaatg atgtggtact aaaaaagatt caatctgaat ttgaaagttt gaatgataca
     7261 atcgagaata cattagccgc ttttgattct aatccagctg tcaaaagcta tttttccaat
     7321 gatccagccc aacatatgga acagcttcag caactaagaa caatccaaaa aatgaatgac
     7381 tctctttcaa aaatccatcc aatgatcgat tacgatgtat tgatctttgg agaaaatgga
     7441 agaacttttg tcggaaacga catgctcact gccgtatctg ccgattcttt ttttcaatct
     7501 gcgattgctc agagagtgaa tgaacgtgct gctgatacac aaatgctttt tgcacaccac
     7561 ggattgactc ttcgcgataa gaaagcaccc agcgtcttct ttgtcaaaaa actgaaaaat
     7621 acattgaatc acgtttacgg atatgctgtt ttatcgatct catccaagca actagcgaat
     7681 ctttttcaat ctgtagtaaa tcctgagata tcgaagatct ccttcatgaa cgaagaacag
     7741 acgatcatcg catcaaatga agaaaataca attgggagcc gatcaatgat tttcaaaaaa
     7801 ctaacagtag gtgaaacaac gatttcaaat ggacagcagt tgacccgtct tcctctgtac
     7861 cgccaggatt gtacattact cagtcaagtc gatcttcatt ctttatctaa ccaaatgggc
     7921 gtgatttttc cgattatttt gtataatatc gtgacacttg cttttgctgg ctcattggta
     7981 tttatgtatt tggatcgaca cacaaaatcg atctaccgct tgattgattc gctaaaacgg
     8041 ctagaggaag atggcttaaa agctactgtt cctgtacaag gaagccatga agtccaattg
     8101 ctagggaata cgatcaatca attgctaagt aatgctcagc atcattatga acagacgatc
     8161 caagacgaaa agaagaaacg ttcgcttgaa atccaaacta tgcaagcaca gatacagccc
     8221 cattttattt ataatacgct gactagcttg aaatttttga tttggcagaa aaaaacagat
     8281 gaagcagttc aaggaatcga cagtttcatt actttactaa gaagtacgat tgggaaaaaa
     8341 gaagaagtga ttccagtcaa ggaagaaatc aaaagcgtac aaagttacat cgatcttttg
     8401 agtatgcgat atggcgaaag aatctcggct aaaataatga ttcctgatga actgcagtca
     8461 ttatctatgc cgaatatgat ccttcaacca attatcgaaa acgcctatct tcatgctttt
     8521 cacaataaaa cgcaaggatt catcacggtg tacggcaaaa caaaaaatca gcttctttta
     8581 ttcgaagtga ttgataacgg cgatggcttc gatactgaaa ataccaaaca aaaaaatgat
     8641 catttttcag gaatcggcat cagccatgta gatgagcgga tacgcctaat gtatggcgat
     8701 gcatatggct taacgatcca atcagtgatt gggacaggaa cgactgtaac gatttgtcta
     8761 ccgcttcttc ctaaaaatca atcagaatat caaaaataaa gaatagccaa gcgctgattt
     8821 ttttatttta agttaaataa atcaatcagt attcttgact ttcatctacg cgtctcttct
     8881 actgtcagca ctggataaca gcccaatatt tgactttctc ctccgagcat cttgatttct
     8941 tggagcgcat tctctactaa tgtcattgga cggtccaaca acaaatcgat cacgaaaaaa
     9001 tattctccca agctagtttt caatggtcgg gattcaatct tacttaagtt gatttcccgc
     9061 cagccaaaag ctgccagcat tttatgaagc atccctggcc gattagcagg tagtgtcaag
     9121 atgacactca tttttactgg agctgactgc tggctggtca tctttcggtc accgacgatc
     9181 caaaaacgtg tctggttcaa ctcattatct tggatattct cagctaagat ctccaatcca
     9241 acatgctgtg ctgtttctaa tgaagcaatc gctgctgcgt cttctttcgg atgttctgct
     9301 acatacattg ctgcagcagt tgttgattct gttgctacta aaggaacatt aggataatgc
     9361 gtttctaaaa actgttgtga ttgtgctagt gcttgtggat gtgaaaggat ttttgtaatc
     9421 tttgttgctg gattgcctaa aagctgctgc ttgataggaa gaacgatttc acttttaact
     9481 tcgacttctg gatgtttgga aagaagatcg attgtgtgat gaacagctcc ttccaatgaa
     9541 ttctctacag gaacgacagc cagatcgact tgtttgcgaa acaaagcgcg caaacaaaca
     9601 gggatggaag catacgcagt caattgttct gtaggaaata gctgacaagc cgcctgataa
     9661 gtgaatgaac tttctggccc tagatagctg acgttcatat gcttttgatc ctttctataa
     9721 tggtatcgat gatcttttct ggtgtttgat tggtcgtgtt gattcgatgt gtcgcacttt
     9781 cttcatacca agaaagtcgt tgtgccaata atgcttttat ttcttctctt gtttttccaa
     9841 gagctaaagg tcgaatagac gtttcatctt gttggatacg atcaactaga cagtccgctt
     9901 ctgcctctaa gtaaagaaca atcggctgtg tttttaaaaa acgccgattt tctgcacttt
     9961 ggacaatccc tccaccagtc gcgataattt tgtcttcatt tgacagcttt cttaaaatat
    10021 ctgactccac ttttcgaaag cttttttcac cgtaatagtg aaaatattcg gcaatcgaca
    10081 attggatatg ttcctcaatc tttgtatcta aatctacata ctctttttgt aaacattgag
    10141 ctaacccttt accgattgtt gtttttccag cgcccatgaa accaatcagc aaaatgctag
    10201 ccatgagctt ctcctcctga cacaagttct gctaaatcgt caaaaaatgc cggataagag
    10261 attgaaacag cttcagctcc ttctagttcg caaggtccat cagctatcaa agcagctact
    10321 tgcagcatca taccgattcg atgatcgcct ctgctggaca cttttgcgcc atgcagaggt
    10381 gtcggcccat ggatgatcag accgtctgct gtcgctttta tctttgctcc cattttctgt
    10441 aattcttctg ccaccgcatc aattctgttg gtttctttta cttttaattc ttctgcgtct
    10501 ttgatgatcg tgattccttc tgcttgagta gccactaaag ctagaatggg caattcatca
    10561 atcagacgcg gaatgatttc tccaccaatc gttgctttct tcaaatgact cgtcttgaca
    10621 cttaaatcag ccgattggtt ctgttcattg tgattcgtct gggtaatcgc tgcgcccatc
    10681 tcctccaaca catctaagat accagttctt gttggattga ccccgacatt ttttaacaac
    10741 agttggcttt ccggaacaag aagtcctgct gctagaaaga aagcagctga tgaaatatca
    10801 cctggaacag ttatctgttg accagttaat ttttgcggtc cggtcactat gatcgtttta
    10861 tcttccactg tgatccttcc gccaaattga cgaatcattt cttccgtatg gttacgggaa
    10921 cgttcttttt caacgattac ggatgtgcct tctgcttgta atgccgcaaa cagcaaagca
    10981 gatttgactt gcgcactggc tacaggcata ttgtatcgga taggtgataa agagcgggtt
    11041 cctgtcacag tgattggtag atactggtca ttttcttctc cttgcaagtc agcgctcatc
    11101 tcgcgtaaag gttccatcac tcgccccatt ggacgtttag acaacgaagc atccccgaac
    11161 agagtagtcg taaaaggctg tccagccaaa atacccatca ataaacgtat ggtcgtaccg
    11221 gagtttccca tgtcaattga ttcggttggt tggacaaatg agtggctacc gcgaccatga
    11281 atcttgatca cctcttcctc ttcttcgatc tcgacaccta attgttggaa ggcatgtaaa
    11341 gtactcagac aatcttctgc gcgcaaaaaa ttttgtatag tcgtcgttcc ttcagcaatt
    11401 gctccaaaca tgatgctgcg atgggaaatt gatttatctg ctggtatcct aacagtccct
    11461 cttaatccat gtatttgttg taataattgc atttatccag cctcctattt gatccagcaa
    11521 tgataactgg tattagacat aatgattctt tttgcttgta acaaatcctg ttggttttta
    11581 aaactgattt gcagtattcc taaaatatct tccctggtct cttggatctt caaattaatc
    11641 aaagaaactc ccgcttttcc gatcagtcca gttacttctg caataacgcc aggttcatct
    11701 ggcacatcga taaataaatc aaaaaaggca ggaatcgcgc cttgcttatg gacaggcaac
    11761 tggtctcgac tttcttttgc ccgattgaaa aaagaaaaga tttccgattg gttctcagtc
    11821 atgatccatt ctgctatttg tgtcatttct tgttgccaat ctgccaatcg ttccaataat
    11881 atctgcttat tgctcaacaa aatatctgtc cacatgacag gatctgagga agcgatccta
    11941 gtgatatctc gaaagcctcc tgctgccagt tgtttggctc gcggatactc ttgattgaat
    12001 tgttctgcct gattgactaa gccagctgcc agaatatgtg gaaaatgact tagcattcct
    12061 gttatacggt catgttctct tgctgtaaga gttacatatt ttgctttggt tccgcaaaat
    12121 agttcttgca gtttttcaac atcttttttc gtcttttcca aaggtgtaaa gatgtaatag
    12181 gcattttcaa atagtttagc atctgctgcc tgtatacctg atttatgaga accagccatt
    12241 ggatgtccac cgacaaaccg aagatttttc tgctcagcgg atgtcacgac ttgctgcttc
    12301 gtactgctga catccgtaat gatgacgtga tcagacaagt tgattctttc aaggacatca
    12361 agataagcta aggttgtacg gacgggaacg gctaataaga taacgtctgc ttgttctgca
    12421 cctgtttcaa aatcacttgc aacgtaatca acgatctgct gttctactgc ttgctcacgg
    12481 gtttttgact gactatccca accaactact atcagttctg gattggtctg acgaatacaa
    12541 agtgccatag aagcaccaat cagtccaagc cccacgataa aaacatgttg tgccatctta
    12601 tttgccccct ttttagtaat gtttcaagta gtcacggtac tccttcactg cagctttcaa
    12661 ctcaacaagc gaatcacttg ggaattttga caatagctct gtcgctacca cagtggctac
    12721 aacattttcc gcaacgacgc tagctgcagg aactgctgta ctatccgaac gttctacact
    12781 tgctttgtaa ggttcttttg tatcgatatc aacactttga agtggcttgt acaatgtagg
    12841 aatgggtttc atgaccccgc gaatgacaag tggttcgcca ttggtcattc cgccttcgaa
    12901 gccgcctaga ttattggtac gtcggaaata gcctctttct tcttcccata ggatctcatc
    12961 catcacttct gagcctggta atgaaccaga agtgaatccg accccaaatt ctacgccttt
    13021 aaaggcattg atactaacca cagcttgtgc gattttggca tctagcttcg tgtcccattg
    13081 tacgtagctt cctaatccag ctggaacgcc tccaacgagc acttctacaa ttcctccgat
    13141 cgtatctcca tttttctttg tctgatcgat caaatctttt attctttgct caactgctgg
    13201 atcaacaact cgtacttctg acgcttcaga aatttctttg atttcttgaa tcgagatatt
    13261 tccaggtatt tctgcttgga ttccaccaag acaaatcacg tgtcctgcta cgatgatgtc
    13321 aagttcttcc agaattttct tggcaattgc gccgattgct acacgcatgg tcgtctctct
    13381 tgcagaggac cgttcaagca cattccgaag atctcgatga tggtatttca taccgcctac
    13441 caaatccgcg tgtcctggac gcggacgtgc aactcgccgc agtgattggt ctttttcagg
    13501 cacttctgct acagacatga cagttgtcca atttttccaa tctttatttt ctaccactaa
    13561 agtgacaggt gaaccaattg ttttgccgtg acggatgcca gacgtgatcc gaacacgatc
    13621 tttttcgatc ttcatccttc ctcctctgcc gtaaccagct tgtctgcgag cgagttcgtg
    13681 attgatttct tcttctgaca gaggtaatcc tgctggtagt ccttcaataa tggctgttaa
    13741 ttctggtcca tgggattctc ccgcagtcat ataacgcatc ttacagtcct cctaagtaat
    13801 ctttgaatgt ctctgtggga acaggaacga tttttgcttg cccaatcttt tctagcaaga
    13861 tgatattcaa ttggcttcct cttgctttct tatcatgggt tatcgcgtgg aataattcat
    13921 ctggatgact tagatcgtac gtaatcggca gatggaattt ttgaagtatc ctttctaaag
    13981 cagctgtcga tccagttgga gacaagccga ttttttctgc atgagaagtg atggctacca
    14041 ttccaatagc tactccttct ccatgactga ctaccccata tcctgctgta ttttcaattg
    14101 catggccaat tgtatgacca aaatttaaaa taagtcgatt tccttggtca aagacatctt
    14161 cttccactac ttttcgtttg atttccaacg tagcagtaat gattggaaca gcatgttcca
    14221 gaagatcttt ttcgtctttc agctcgttca gctgtttcca aagcgttgca tctgctatag
    14281 ctgctgattt gatgatttca gcgattcctt ctcgtaaacg acgtaccggc aaagttttta
    14341 gaacctcagg atcaatcaat acaccagctg gctggctaaa tgttccgaca aggtttttcg
    14401 ctttgtttgt attgactgct gtcttcccgc caatactaga atctacttgt gcaagtagcg
    14461 tagtcggaat ttgaacgaaa tggatcccac gcatgaacgt cgaggccacg aatgctgcta
    14521 gatcacccac cattcctcca ccaagtgcaa gtatcccatc gctgcgggta aaatcattat
    14581 ctgctaaaaa atcgtataac tcagctgctt gttctaaaga tttactggtc tcacccgccg
    14641 gtacgattga aaggactggt tcaaaattag cttgtctcaa ttggaacatg attttttctg
    14701 catatagtgg agcaacgttt gtatcagtga tcacagctac tttttgaggt ctccaaatcg
    14761 tttgaagcca tgatccagcc tgttctagtg cgcctttttc aatatgaata tcgtattttt
    14821 ggttatcaac atgtacagta agcattcgtt tcacttcttt cttttaaaaa gctggaatta
    14881 atcgagtgtc tgaatctttt ccattgcttc tttcatgatc gcaacttctt tcatcatgcg
    14941 tgagtaagtc ttttcattca acgattgggg accgtctgac caagcatttg caggatcagg
    15001 atggatctct acgatcatgc catctgctcc gcttgccaca cccgcacgag ccattggtgg
    15061 tacaagttcc caaattcctg taccatgact tggatcaaca atcacaggga aatgagttaa
    15121 ttttttcatc aatgggacag cacttaaatc aaacgtatta cgtgtcgctg tttcgtaagt
    15181 ccgaatccca cgttcgataa aaatgacagg agatttgtct tcgactgcaa tgtattctgc
    15241 agcattaagc cattcattga tcgtaccgga aattccacgt ttcaatccaa caggtttacc
    15301 tgttttaccg acagcagata gcagtttaaa attttgcata ttacgtgcgc caatctgtag
    15361 gatatcgctg tactcagcca ccatatcgat atgcccttca tccatcactt cagtaatcac
    15421 tttcattcca aattcatcag ccgcttgacg gatgtatttc aatccttctt cttctaaacc
    15481 ttggaaagca tatggagaag tacgtggttt gaaggcacca cctcgcagaa tttttgcgcc
    15541 cccggctttg gccattcgcg cacattctct gatttggtct aatccttcga tagaacaagg
    15601 accagccatc gtcacgaaac tgccgtcacc aatttttact ccgtctacat ccacgactgt
    15661 attgccagga tggaattctc gagaagtcaa tttgtatgtg tttaaaatac gcacagcatt
    15721 ttctactcct tcatagcgtg taaatgctcc ttcttgtaaa cttcgcgtat caccttttag
    15781 tccaatcact actcgttctg ttccttgatc gattgagact ttgaatcctt ctttttcgat
    15841 atgatcgacg acttttttta tttgttcttc cgttgcttct tttttcataa ttactatcat
    15901 tgtttttccc gctttctttt gtttacttat ctatcttggc tgattttctc tactttttca
    15961 aggtggtttt acattaattc ctactaaatg accgtgtcct gttgttctca ttttcgctcc
    16021 ttctttccta cttatgaccc agataatcaa caaaaaaaga tgtccctgta ggaaattgcc
    16081 tctacagtga acatcttcaa cgagccttaa aaagcgctgc aaaaatataa aatagccact
    16141 atagatgaat ttctctacgt ggctgtaagt cgtgtttaaa aatctttagc catcatagat
    16201 acaaacctgg tttgtttgta cccacgaagt catgaataca aacctatcta aaatagctaa
    16261 agtaatacgt attcagtttt gtaagttttc tagatttcat aaaaaatcgc tccaaactga
    16321 tgtaagaatt gtacctagta tagacat
//
LOCUS       DAKBVG010000045        16228 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000045 DAKBVG010000000
VERSION     DAKBVG010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.045"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(544..2175)
                     /locus_tag="OUD09_001027"
     CDS             complement(544..2175)
                     /locus_tag="OUD09_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HCT9692310.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(2189..3862)
                     /locus_tag="OUD09_001028"
     CDS             complement(2189..3862)
                     /locus_tag="OUD09_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HCT9692311.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(3875..4192)
                     /locus_tag="OUD09_001029"
     CDS             complement(3875..4192)
                     /locus_tag="OUD09_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCT9692312.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(4217..5617)
                     /gene="lacG"
                     /locus_tag="OUD09_001030"
     CDS             complement(4217..5617)
                     /gene="lacG"
                     /locus_tag="OUD09_001030"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HCT9692313.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            5854..6639
                     /locus_tag="OUD09_001031"
     CDS             5854..6639
                     /locus_tag="OUD09_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCT9692314.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            6642..7334
                     /locus_tag="OUD09_001032"
     CDS             6642..7334
                     /locus_tag="OUD09_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCT9692315.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(7608..7979)
                     /locus_tag="OUD09_001033"
     CDS             complement(7608..7979)
                     /locus_tag="OUD09_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HCT9692316.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(8077..9690)
                     /locus_tag="OUD09_001034"
     CDS             complement(8077..9690)
                     /locus_tag="OUD09_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCT9692317.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFERPSFQRMIEDIEVGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAYYGA
                     YAPLGYKKHPDIKGKLLVDEETKWIVEKIFSLAYQGYGSAKITKILREEKVPTSSWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAQIKGILKSEVYIGNSVHNRQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIEKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     ATNKANKTPYSYYACSFYGQFGKGYCSMHYIRYDVLYQAVLERLQYWAKAVQQDEEKV
                     LHKIQKVGNAERIREKKKKASALKKAENRQNEIDCLFAKMYEDRACEKITERNFIMLS
                     GKYQKEQIELEQQITDLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEATTNEENERIQEIEIYYRFIGKVD"
     gene            complement(9802..10012)
                     /locus_tag="OUD09_001035"
                     /pseudo
     CDS             complement(9802..10012)
                     /locus_tag="OUD09_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
     gene            complement(10731..11945)
                     /locus_tag="OUD09_001036"
     CDS             complement(10731..11945)
                     /locus_tag="OUD09_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HCT9692318.1"
                     /translation="MNDKNFIEELRQKREEYGVTQTRIAVACGISREYYNRIEKGKQP
                     LNNELKEIIEKQIERFNPREPLFLLIDYFRVRFPTTDALKIIRDVLQLKADYMLYEDF
                     GKYGYESKYVLGDINIMCSMQEHLGVLLELKGRGCRQMESYLLAQERSWYDFMLGCMT
                     AGGKLKRLDLAINDKAGILDIPKLIEKYKAGECISYFRKQKDYGGTEKNGYDTPQNTG
                     ETLYLGSTSSELYMCAYQKNYEQYVKNGTEVEDTEIKNRFEIRMKNERAYYAVVDLLT
                     YRDAERTAFSIINHYVRFVDREDDKPKSQWKMNDDWAWFVGENREPIRLTTKPEPYTL
                     QKALHWLQRQVAPTIKMVQALDKENHTTILKDMIEQAELKDKHKHLLQLEKSTIEERI
                     DTVVPQENDGIF"
     gene            complement(12564..13031)
                     /locus_tag="OUD09_001037"
     CDS             complement(12564..13031)
                     /locus_tag="OUD09_001037"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HCT9692319.1"
                     /translation="MNTFEGNLVAENIKIGIVVARFNEFITSKLLAGALDNLKRENVD
                     EKDIEVAWVPGAFEIPMIASKMVKSKKYDAIICLGAVIRGSTSHYDYVCSEVSKGIAQ
                     ISLNTETPVMFGVLTTDTIEQAIERAGSKAGNKGSECAQGAIEMVNLIRALEV"
     gene            complement(13032..14237)
                     /locus_tag="OUD09_001038"
     CDS             complement(13032..14237)
                     /locus_tag="OUD09_001038"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HCT9692320.1"
                     /translation="MKYKKIKEALQALQEGKLVLVMDDKERENEGDLICSAQFATTEN
                     VNFMATYAKGLICMPMSESLANRLMLSPMVENNTDNHETAFTVSIDYKDTTTGISAEE
                     RGLTARMCVVEDINPFDFRRPGHMFPLIAKNGGVLERNGHTEATIDLLRLAGLKECGL
                     CCEIMNENGKMMRTSDLIQFSQIHHIPTLTIKELQEYRKVYDMLVECVSVVEMPTKYG
                     DFKAHCYINKLNGEHHVALVMGELNSGNDVLCRVHSECLTGDVFGSLRCDCGQQLDRA
                     MKMIAENGSGVLLYMRQEGRGIGLVNKLKAYHLQDKGMDTLDANLALGFQGDLREYYI
                     GAQILRDLGIKSLHLLTNNPDKVYQLEDYGMQISSRVPIEIKANPYDSFYLKTKKDRM
                     GHILNMEEK"
     gene            complement(14257..14892)
                     /gene="ribE"
                     /locus_tag="OUD09_001039"
     CDS             complement(14257..14892)
                     /gene="ribE"
                     /locus_tag="OUD09_001039"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HCT9692321.1"
                     /translation="MFTGIIEEIGKVERIQKDTYNCKLSIKSSKILEDIHLGDSIAVN
                     GICLTVTCFTRQSFIVDVMNETWNRTALNLLKHGSLVNLERAMHMNGRLGGHIVTGHI
                     DGTGRISSVRRDKNAVWYQINTQREILNLIVEKGSVTIDGISLTVAKVSKADFSVSVI
                     PHTLEQTILKNKQVGDMVNLENDILGKYVKKLIGNSNETELSKELLCRYGF"
     gene            complement(14877..15977)
                     /gene="ribD"
                     /locus_tag="OUD09_001040"
     CDS             complement(14877..15977)
                     /gene="ribD"
                     /locus_tag="OUD09_001040"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HCT9692322.1"
                     /translation="MSDIEYMKIAIELAKKGAGYVNPNPMVGAVIVKNNRLIGQGYHE
                     VFGGLHAERNALKNCRESPVGATLYVTLEPCCHYGKTPPCTETIIKSGITRVVVGTLD
                     CNPIVSGKGVKQLEENNIQVDVGILEIECQQLIKVFRKYITKRIPYVFMKYAMTMDGK
                     IATYTNQSKWISGEKARKQVHQFRHKVTAIMVGVNTVIQDNPLLTCRLENGKNPIRIV
                     CDTDLRTPITSKIIQTANDIKTYIATSSIDESKIALYRKCGCEIIYTKKKGNHIDLMN
                     LMQCLGNMQIDSLLLEGGSALNWSALEQQIVDEVQTYIAPKIFGGSGKSPVSGQGVVF
                     PKDAIMLKPYAFSQVGNDYLIESEVIYPCLQE"
     regulatory      complement(16113..16227)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
BASE COUNT         4685 a         3582 c         2607 g         5354 t
ORIGIN      
        1 atagtatcgc attctgtttt atacacttta cgttgaaaac agagtgggaa taaagagcca
       61 acataatatg gagggggaat ccctataaat caacattctt caatagctaa aatgcttgat
      121 tttcattaaa aataagatgg ttccctacca aaagagacta taatataagt taatttgttt
      181 cattcttatc ataaaaagaa gttctcgaaa aacgaactgt tttccgagaa cttttttcta
      241 tccatctaag tgctcaaatc atattcacga cggaattcta aggttaccct ttcttaaaca
      301 aactttatgt tatctgcaca actttttgca attttttcat tgcttcttac gaaatcgttg
      361 aactctcttt agcataatac aaagtgtaga tgaagtctgg tgctttactc gttttggtaa
      421 aaatgactgg aaatcgtttc tggttatttt tggcaaaggc gaggtgcagg ttcgattcct
      481 gccttctacg ctaacgatgt ggctgaaggg aacctccgat cttgaatcga aacgatccgc
      541 agtctattgt ttcaccaatg aaacaaaatc aacgttcgtc caagcatttc ctgaagcctt
      601 gctataaacc ccaatttcac aaacacctgt tgtaattgga atgtctttga ttgtaatttt
      661 tgtccattca cttgttttaa caaccgcagt atttagttcc gctccaccat aatttttgat
      721 atataactga ttagtatcaa actctccccc agacattaca taggcactca atgtgtaagt
      781 tcctttgctt atgccactaa cattttgata agctgatgcc tcgtatgctt gatcctgcca
      841 aaaagttagt ttgtagtccc caaaaaaagt gcccgcttcc gttttgatcg tacccttagc
      901 ggttccttgt tgaaaccagg tttgccaatt ggaaggactg gtcgttggtc catcgctttc
      961 gaaacttgca tttttaacta gatttatttg cgcccctgct tgtttaaacg cacgcaagct
     1021 tggcaaggcg tttccagcaa aatcaaacat cgcttggttg tcccattcgt ttcccatagt
     1081 ggattgaaca ctgagataat tcatacctgc ttgtgttgcc caactcacat ttccgttgta
     1141 ccaaagaggt tcccaataga agaagcccgc cccgcgatta ttcggtactg cctgaatccg
     1201 atcggcaaga tccagcaaat actctgcttg tcctgtcggt gttggttcat atccccccgc
     1261 agctgcttcg gcttcaccaa aagcatttgg catagtatcg gcattcgctg ttgtatgccc
     1321 ataagctgtt tcaactacga tcacttcttt accataccgt tggctgatcg cattcatatt
     1381 ggtttgaagt tcattcattg aaccgtgcca atacggataa tacgacactc caattaaatc
     1441 aaatgacaca cctcgactcg tgatttcatc aaaccaccaa gtaaatgctc ctgtatcgcc
     1501 accatcagct aagtggagca ttactggagt tgttttaggt tgagtgtctc gaacagcttg
     1561 gataccagac ttcaaaaaag cagccaatcg atcaaattct cccccgcctt ctccccaaga
     1621 cttgccataa ggccagagca ttccgctatt cagttcattg ccgatctgaa ccatatcagg
     1681 ataaacgtcc agctgcttca tttccgacag aacgttcttt gtatagtcgt acaatgccgt
     1741 attcatttct tcaaaggtca gaccttgcca tgctttagga agattctgtt tcccaggatc
     1801 aacccaaaaa tcactgtagt gaaaatccag cagcacctta agtcccagat tttttgctcg
     1861 ttttgcaagg tcaatcgtcg tttccagatc atttgtccct gctccataac tttctccatt
     1921 actattataa ggatcctgcc acaaacgcag tcgaacatag ttgacaccat tattttttaa
     1981 aatctctaag gcatcttttg aaatatcgtc ttcataatat tttgcaccag atttttccat
     2041 atctgacagg atcgaaatat ctgctccgcg aataaattcg ctggcagatg cttcaacagt
     2101 ttttgaaaca gcccccaaag cccctaacac agctgcaaat aaaacagctt tacgcaacca
     2161 aattcccatt ttcattatgg ctcctccttt attgaaattg gttcagaata aactgaacag
     2221 ctttttcagg atctctcgtt aatccgatat attctgctcc ttttgtcgca accagcttga
     2281 ttcctaagcg atcggtgtct tctttgatat catcatagta agaattgact tgcggtgcta
     2341 agaccaccat atcaaaatcc tgcagcattt cgtaatgaga accataagca cccgccattg
     2401 cactgatcgg tgctttcgat tcagatgcac ctttttttaa agcattagct aacatcgcac
     2461 ttgtccctgc gccagcacaa agtaccaaca cattgatttc tttatttgca tcgctgataa
     2521 cttgactcgc tgccacaggt acttcctcac gtacatcggc agaagcacct tcctcggttg
     2581 cgatcacctc tgctttttgt gcttctttct ctactaagac agcatcgtat gctttgcaaa
     2641 atggtagata gatcaggaag tcaacgacga ttagaaccgc tgctagaaca aaagctaaga
     2701 tacccatgcc cgttcctaga accaaaccga tcggtgctgg agttgcccaa ggcaaaacgt
     2761 acataaagct gttcatgccc aatacatcga caaaaaactt gaacaggcaa acgtttacaa
     2821 caggtgttaa caaaaatgga ataaaaaaat aagggttcaa aatcatgggt gccgcaaata
     2881 gcaatggttc gttgacagcg aaacttactg gaataaagga agctttccca actgccttca
     2941 actgcttaga cttagcaaag agtaggaaga taaaaggaac aaccaatgtt gccccagttc
     3001 ctcccatagt tcctacaaaa ttccctaaac ccaccgtcag tactttagaa gcttgttcac
     3061 cggaattgaa caattgcaga ttggtttcca cattggcata gataattgcc gcaattgccg
     3121 gctccacgat cgacggtcca tgaacaccga caaaccaaaa cagcgccatc gctccccaga
     3181 taatagcgat tcctaagtac ccgtcagctg ctgtaaatag cggctgcagc accgtaatga
     3241 ttgcttctgc aaatgagatt tggaagattt gtcgaatgcc taaatcaatt aaaacggccg
     3301 caaatactga aaaagagaaa ggaaaaatat ctcggaaggt ttgcgaaatc gttccaggaa
     3361 cttctttagg cattttaatg gtaatatctt tcagcacaca aaacttatat aaattaactg
     3421 ttaaaaatgc agcgacaaag gaagccagta gtccctttgt ccccatatat tccgttgaaa
     3481 atccaccttc aatcggagag acgcttaaca acaggaaact taccacagag gccagcatca
     3541 cagaggtggt gttgattact ttgtttttcg gtaagcgacg attcatcgaa tcagccaagc
     3601 agcgagcagt ggttgctgaa acgagtaacc ctacaacacc catcgaataa ttatagactt
     3661 tccatagcca tgtggagaca gattcaggcc attggtaacc aaaaatctca ggaaccgcag
     3721 caatcaaaat gaaaatgctt gaaaataaaa tagctggcat cgctgttaaa aatccgtcac
     3781 gaacggctcc taaataacta ttacgtgaga ttttttcaaa aaatggtttc cctttttcga
     3841 tttgtttaat gattccatcc atagcgttct tctcctatcc ttgattctgt ctgtacagta
     3901 caataaagtc ttgaatcaga tctctcagca ataaagtggt cattaaatga tcctgcccat
     3961 ggataaaaat gaatcccatc tccatctctt tcccagatgc ttcttccgct aaaatttttg
     4021 tttgcgaatt atgtgcaagg gttaaatttt cgtcggcttc tttcaacgcc gaatcaacat
     4081 gttcaaagtt tccttgtctt acttctttca ataatttcaa taatgtactg cgagcgtcgc
     4141 ctgaatatgc aacgatctca aaaccaagca tttgtaattc ctctttattc atcttttctt
     4201 cctccattat cacttattaa ataatcgttt tggtttcact gaccaacttg taccagtaag
     4261 ctgattcttt tgggtaacgc ttctgggttt caaaatcaac ataaaacaag ccataccttt
     4321 tgttataccc atttgtccat gagaaaagat ccataagcga ccacaaaaaa taccccttca
     4381 catttacccc ggctgttatc gccttgctta aggattccaa atagactctc aaatagtcga
     4441 ttctcggctg atccataata atgccatctt caaactgatc tttatacccc atcccgttct
     4501 cagtgatata gattttgttg taatgtgggt aatcgctttt aattcgcagc aacaagtcat
     4561 atagaccttc tggataaatc agccagtccc aatcagttcg aggaattcct tctttataaa
     4621 tacgttctcc gattcccttc actttataaa cagaggtccc tttttcaccc gtaccgttat
     4681 gatggattgc attttctcca tcgtaagctt tgacaaaatg gcattggtag tgattgatcc
     4741 ctaagtagtc attacgcgtg gatgcttttt tcaattcaac gaaatcttct tcaggaaaat
     4801 gatagctggc ctgatttgct tcacatatct catcaagagc agtcaaggtt tcagttgaat
     4861 aatagcctaa ataggtagca tctaataaga aacggatcga caatgcatcg tctaaaaagg
     4921 cagcatgctt atcttccggc gcgtctgttg ccgcatattt cgtctctaat gagtgaacta
     4981 caccaatttc acccggaagt tcgttttctt tgaaatagtt cactacacgg gcatgagcaa
     5041 ccatcatatt atgaagacag gcgacgatct ttgtaaaatc atatttgatt cctggtggga
     5101 aaacacctaa caaatattga ttggttgcaa ctggatagat ttcattaaag gtactccaca
     5161 ccttgacttc tttgaattca tgaaaacaaa aaatggcata ggaaacaaat gcttctattg
     5221 tttctcgatt caaaaaatct ccatggtcaa acaaacgttt aggggtatca aaatgatgca
     5281 acgtcacaaa cggcgttacg tttcttttct tacattcttc gaacacacga tgataaaaag
     5341 cgattccttc tggatttggt ttcccagctc catcaggaaa aatgcggctc caagcaatgg
     5401 ataatcttag accatttaca ccaaaacgtt cacacaattc aatgtccttt gcatattgat
     5461 gataaaaatc acttgccgga tcgggactga atcgcccttg ttcctctaaa aagtcatccc
     5521 aagcaacagc gcctttgcca ccttccttag tggctccttc aacctgatag gctgctgtgg
     5581 ctccgccaaa aataaaatct tctggtaatt taagcatgta catctctcct caaatctata
     5641 atcagttttc gctctttcta actgattgca ttgtaaacgc atccaaaaca tttgtcaaca
     5701 aaaacaaaca caaataatca taaaaaccag aaaacagcaa taaaatctat tgtaacaaac
     5761 ataaaaacca ccctaaatgt ttacttttgt tcgaattaga ttgactgttg tccgtttata
     5821 tgatagatta tccttaaatt agaggtgaag acaatgttga aaaaagaaag attgcttact
     5881 atcgtagaaa tggtcaataa aaaaggaatc cttaccgtta atgaaatcat caatcaatta
     5941 gatgtttctg atatgaccgt tcgcagagat ttagatgaat tagaaaaatc gggcaagctg
     6001 ctgcgtgtcc atggaggggc tcaaagtctt tcctacactt tagatcaaga actttctcac
     6061 actgaaaaag ccactctgca gattcaagaa aaaaaagaaa ttgctgccac agcagcctcc
     6121 ttgattcacg agggagaaac gattttctta ggaccaggta caacaatcga actattggcg
     6181 acgcatttac tgaacaagaa aattcggatc attacaaata attatccagt ttttgatatt
     6241 cttgctcaag cagatggacc agaaattcta ctgattggcg gtgatttccg gaaaaatacc
     6301 ggcgcatttg tcggaccgat caccaacgac aatttaagaa gactaaagtt tacaaaagca
     6361 tttatcagtt caaatggcgt tcacaacgaa gcgatctcta cttacagtac cgaagaagga
     6421 gaagcacagc agatcgctct aaataattcc cgtaagaaat atctactcgt tgataataaa
     6481 aaattcaatc gagatgattt ttatgtgttt tataatttgc atgatttcga tctgctcatt
     6541 acggatcgcc agatctcgcg ggaggttcaa gagcactatg cacaatatgt cgatctgcaa
     6601 attgctgatt caaccgaaaa taccactata ggagcgtaac tatgaaaggg attatatttg
     6661 atttcaacgg aacaatgttt ttggactctc atctgcatga agctgcttgg ctgcatatga
     6721 tacatgatca tacaaaagat ggtttatcag acgaggatat tttaaaaaat attcatggtc
     6781 ggacgaatac ggagattttg aaacatttca tttcagagca tttgaccgcc tctgagatcg
     6841 ccgtattgtc agaagaaaaa gaaagctatt atcgtatgct ttgcttgcaa aatgaaaacg
     6901 agcttcagtt gacaaaaggg cttgaagcga ctcttgacca gctgactgac caacagattc
     6961 cattaacgat tgccacggct actgtcaaag agaatgttgc tttttatttt gatattttcg
     7021 atttagcccg ctggtttgac tgggacaaga ttgtctatga cgacggctct ttccctggga
     7081 aaccgcaacc ggatattttt cttaaagcgg cagacaaact gagcttagct ccggctgatt
     7141 gtcttgttat cgaagatgct tactcaggat tacttgctgc caaacgtgcg gggatcggaa
     7201 caattattgc aattgatcca ttcggtaaaa acaaaacagt atttgaacag gcacagctaa
     7261 ataaagatgg aaccatcaaa gattttactg gtttttggac agagcacctg tctgccacat
     7321 ccgttaattc ttaaacgatt caactaacta ccaaagaaaa gcaactgaaa tgcacacctc
     7381 cttttataaa aaactgccag tgaaacgaca tgaaagatac actgctatat ccttagtgaa
     7441 atatagcagt gtatctttga ctttttcatt cttttaacca tatgacgcgt tcatctctat
     7501 ctataattaa cattaaaaat agtgttttca cattatttct caaaccaatc ttaaaaatca
     7561 tcaggagtac ttttttgata tacttttatt aatgctgggc gtaatggtta aaagatttcc
     7621 actagttcct ctccaccaag ctgagttgaa aaatgcttat ctgtaaacaa catagacaaa
     7681 gaggaaataa acgtttccgt atctttctct gtcccaagga tattaattaa ttttcctact
     7741 aggtaagtat cattaaactt aagatctgcc ttcacatcaa aagttttgat aaaagctttt
     7801 atcatgcgta caattttgtc tttatcgcca taattgattt caccactcat atcaatatag
     7861 tcactaatcc ctgaagaaat aatggcaact gcttcttctt tcgccttatc agataattca
     7921 tttaatgact gggattgatt gatgacttct tgagttttat ctgttacgta atctctcatt
     7981 atcctatcgg catactccgc aaagtaatct tgaaaaaatt cttctacagg gtaaccaact
     8041 tccgtctccc ttagttaaag atatgaaccc tatttatcag tctacttttc caataaatcg
     8101 gtagtatatc tctatttcct gtattctttc gttctcctca ttcgttgttg cttcgtgaat
     8161 gagtattttt tctatcattg catttaataa cggtgctgtc agttcctttg gtacggaata
     8221 ctccttgatt aactcaatcc atttttcagc acctatcata tcctgttcca ttttacttaa
     8281 ttcttctctt aagtcggtta tctgctgttc cagttctatc tgttcctttt gatattttcc
     8341 cgacagcata atgaagtttc gttctgttat cttctcacag gctctatctt catacatttt
     8401 cgcaaataaa cagtcaatct cattttgtcg gttctcggct ttcttcaagg cacttgcctt
     8461 tttcttcttt tcccgtattc gctcggcatt accgactttc tgtatcttat gcaatacctt
     8521 ttcttcgtcc tgctgtactg ccttagccca atactgcaat cgctccaata cagcttgata
     8581 cagcacatca tagcggatat agtgcataga acaatagcct ttgccaaatt gtccatagaa
     8641 actgcaagca taataactgt aaggcgtctt atttgcctta tttgttgcaa atctcataga
     8701 ccagccacaa tccgcacact taacaagccc tgcaaatatc ggcgttgcct tttcctttgt
     8761 ctgtctacgc cttgatttta tctgttcctg aacacgatag aacacttctt tttcaataat
     8821 tggctcgtgg gtattttcta ctcgaaacca ttcgctttcg ggctttcgca ctttcttctt
     8881 gcttttgaat gatactgttg actgtcgatt atgaacgctg ttgccgatat aaacttcact
     8941 tttcaaaatt cctttgattt gtgcaatcgt ccactcataa cgcttacttt cgggtttgcc
     9001 ctcaaaaata tgtgcaaaag taccatacct tgtaaaattc aaccaagacg atgtcgggac
     9061 tttttcttct cgcagtattt ttgtgatttt tgcactaccg taaccttgat aggcgagaga
     9121 gaagattttt tcaacaatcc attttgtttc ctcatcaacc aacagtttcc ctttgatgtc
     9181 ggggtgtttc ttatatccta acggagcata agccccataa tacgcaccct ctgcaaattt
     9241 tgtcttaaag gctgatttca ctttacggct tgtatctctt gccacccatt cattgataat
     9301 attcttaaat ggtgcaatct cactttcgcc tttttcgctg tctacaccgt cgtcaatcgc
     9361 tatgtaacgg acattatggc tgggaaaata caattctgta tattgtcctg tcataatata
     9421 gtttctgcca agtcgggaaa ggtcttttgt tacaacacag ttgatttttc ctacctctat
     9481 atcctcaatc attctctgaa aactcggtct ttcaaaattt gttcccgacc agccgtcatc
     9541 aatatattca tcaatgacat tcaaatgatg ttcttttgca tatagacgca acatacttct
     9601 ttgtgtggta atgctggaac tttcgccttg taattcatca tctcggctca acctaagata
     9661 aagtgcagta ttgtaaatct gttgtttcat tgtcagttat cctcctttat aactaacccg
     9721 tgtttacaat acctgcttgc aagtaaagta taacacgggt actttttgcc attcaatcta
     9781 tttcttacaa ctgcaccgaa tttatgcgat tgtctgcttt gctttctcaa gcataacatc
     9841 agttaaaagt tcttccattg tctttccctt ttcggaaaaa tgctctttta caacaatctt
     9901 tgtctttcct ctgtttccaa tgccacactt gttatttttc ttttgaattg tttttacagc
     9961 attatcaata acacataccc ccctttctgt aaaatctttc taagcaattc aacagctttt
    10021 gtcatagccc acaggagttc cacctctgca ttatccaatg tagccgtacc cattgcgtgc
    10081 gacgctttta acgctcaagc tgtggctgta cgggagtatc attatcagac aggacaggtc
    10141 atggcgataa aagaggacaa gtcttttaca agaacacaga taggttgtcg ctcgcttttg
    10201 caggggaaag gtcatggcgt atctgtttca atggcttact gtctgtctaa tgtattgaat
    10261 tgctttattc aattgtcaaa gaacacgaaa gaagaaaatc gtctttccta tataccgcgt
    10321 tggaaaagag caaaagcgta accaatactc aaaacttttt tctttttctg taccatatag
    10381 ggaaagaaaa tgtcattctg ctaagttgat gatgaaaaga tttcttttca caccctaact
    10441 acccatgcaa caccgttact gccaactatg gaatgaaaga ttttctttgc tccatatagg
    10501 gaatggaaac tgctgattgt taagttgaga atgaaaaaaa tttcttcttt tcacataaca
    10561 ctgtccgctt aactcgattt tccgacattt ttcaaaactt ttcttctttc acttcatata
    10621 gggaatggaa acctcgattt aatgacctat tttcaaaaaa gtttagattt ccttttctcg
    10681 taccatatct gtacgctagt tgatgttttg ttgcagattt tttaaagaaa ttaaaaaata
    10741 ccgtcattct cttgtggaac aacggtatct atgcgttctt ctatggttga tttttctaac
    10801 tgtaataaat gtttatgctt gtctttgagt tctgcctgtt caatcatatc ttttagtatc
    10861 gttgtatgat tttctttgtc taatgcctgt accattttta tggttggtgc aacctgtctt
    10921 tgtagccaat gcaatgcttt ttgtaaagta taaggctctg gctttgtggt taagcgtatc
    10981 ggctctctgt tttctcctac aaaccacgcc caatcatcat tcattttcca ttgacttttc
    11041 ggtttatcgt cctctctatc tacaaaacgg acataatgat tgatgataga aaaagcagta
    11101 cgttcagcgt cccgataagt cagtaaatct acaaccgcat aataggctcg ttcattcttc
    11161 atgcgtattt caaaacggtt cttgatttct gtatcttcaa cttctgtgcc attcttgaca
    11221 tactgctcat aattcttctg ataagcacac atatataatt cgctacttgt tgaaccgaga
    11281 tataaggttt cgcctgtatt ttgtggtgta tcatacccat ttttctctgt accaccgtaa
    11341 tctttctgtt tgcgaaaata ggagatacat tctccagcct tatatttttc tattagtttt
    11401 ggaatatcca agatacctgc cttatcattg attgccaagt caagccgttt cagcttaccg
    11461 cctgccgtca tacaaccaag cataaagtca taccatgagc gttcctgtgc caagagataa
    11521 ctttccattt gccgacaccc tctgcctttc agttccaaca aaacacctaa atgctcttgc
    11581 atggaacaca tgatgttaat gtcgcctaaa acatatttac tttcatatcc gtactttcca
    11641 aaatcttcat aaagcatata atcggctttt agttgtaata catcacgaat aatctttaat
    11701 gcgtccgtag taggaaagcg gacacgaaag taatcaatta gtaaaaataa cggctctcgt
    11761 ggattgaaac gctcaatctg tttttctatg atttccttta attcgttatt caatggctgt
    11821 ttgccttttt caatgcggtt ataatactca cgactgatac cacaggcaac agcaattctt
    11881 gtttgtgtta ctccgtattc ttcacgcttt tgtcttaatt cttctataaa gttcttatca
    11941 ttcattcttc aattcctcca atcaaaaaag acaactacca tttactgata attgtctata
    12001 cttaaatttc ttcaaaagaa aaagacaatc aatttttcat ttgaaatcaa ctgtctttcc
    12061 tatgtcttat tcaattttat gtgttaaatg agtgtctttt agttaacacc ctgttaaaag
    12121 cggttttgcc ttgtaaatac agcgttttcc gcttagtagt gtctattgtc tcttgttttt
    12181 tgtgcccccc ttgttagata acgggggctt actgttcgct cgcaagctcg ctcaactgcc
    12241 gaagtgaacc agccactaaa aacggcggaa aaatctaaga gattttccca ccgtcgtgtc
    12301 tggatcactc cgcctgttat gttttttcgc tttagctttt aaccccaagc atattatact
    12361 gttacaacgc attttatttg atgccataat ttgcagaatt cggaaaatta aaacatccca
    12421 tttttagcct tgttataacg gtaataattt tttctattac tgttataaca aggattccag
    12481 gggttcgggg atggaagatc cccgtattat ttctgcacta tttatttctc tgaaaatggc
    12541 atattataat gtgctgatca acattatacc tctaatgcac gaattagatt taccatttca
    12601 atagctccct gtgcacattc agagccttta tttcctgcct tactgccagc tcgttctatc
    12661 gcctgttcaa tcgtatctgt cgtaagaaca ccaaacataa ctggtgtttc agtatttaaa
    12721 ctgatttgag caattccttt tgatacctca ctacacacat aatcataatg gcttgtacta
    12781 cctcgaataa ctgctcccaa gcagataatt gcatcatatt ttttactttt taccatttta
    12841 gacgctatca taggtatttc aaatgctccc ggaacccaag ctacctctat gtccttttca
    12901 tcaacatttt ctcttttcaa attatctaat gcacctgcta atagcttaga agttataaat
    12961 tcattaaacc ttgctacaac aataccaatt tttatatttt ctgctactaa atttccttca
    13021 aaagtgttca tctatttttc ctccatattc aaaatgtgtc ccattcggtc tttctttgtc
    13081 tttaaataaa aactatcata aggatttgct tttatttcaa tcggtactct gcttgaaatt
    13141 tgcattccat aatcttctaa ctgataaact ttatcaggat tatttgtaag taaatgcaat
    13201 gattttattc ccaaatctct taaaatctgt gctccaatat aatattctct taaatcgcct
    13261 tgaaatccta gtgcaaggtt ggcgtcaagt gtgtccatac ccttatcctg taaatgatag
    13321 gcttttagtt tattgacaag cccgatacct cgtccctctt gtcgcatata aagcaatacg
    13381 ccagaaccat tttctgcaat cattttcatt gctctatcta actgctgtcc acaatcacag
    13441 cgtaaagagc caaagacatc tcctgttaaa cactctgagt ggacacgaca tagcacatca
    13501 tttccactat ttaactctcc cataaccaat gcaacatgat gttcgccatt caatttattg
    13561 atataacaat gtgctttgaa atccccgtat tttgtaggca tttctacaac tgaaacacat
    13621 tctacaagca tgtcatatac ttttctgtat tcctgtaatt ctttaattgt gagggttggt
    13681 atatggtgta tttgggaaaa ttgaattaaa tcagatgttc tcatcatttt cccgttttca
    13741 ttcataattt cgcaacataa gccacattct tttaatcctg ccaacctcaa taagtcaatc
    13801 gttgcttccg tatgtccgtt tctttctaaa acacctccgt tctttgcaat cagaggaaac
    13861 atgtgccccg gtctacggaa atcaaaggga tttatatcct caaccacaca catacgagct
    13921 gtcaatcctc tttcctcggc agaaatacct gttgtggtat ccttataatc aatagaaact
    13981 gtaaaagcag tttcatggtt atctgtatta ttttcaacca taggggaaag cataagcctg
    14041 ttagccagac tttcactcat aggcatacag attaaacctt ttgcataagt cgccataaaa
    14101 tttacatttt ctgttgtagc aaattgtgcc gagcaaatta aatccccctc attttctctc
    14161 tctttatcgt ccataactaa aacaagtttt ccctcttgta acgcctgcaa agcctctttt
    14221 atttttttat atttcataat tcctttatcc tttctgctaa aatccgtatc ggcataacaa
    14281 ctctttcgac aactctgttt cattgctgtt tcctatgagt ttcttcacat atttccctaa
    14341 tatatcattt tcaaggttta ccatatcccc gacttgttta ttcttgagaa ttgtttgttc
    14401 caaagtatga gggataacgg atactgaaaa atctgctttc gatacttttg caacagtaag
    14461 actaatacca tcaatagtaa ccgaaccttt ttcaacaatt agatttaaga tttctctttg
    14521 cgtattgatt tgataccaca ccgcattttt gtctctacgc acagacgata ttctgcctgt
    14581 cccgtcaata tgccccgtaa caatgtgacc gcccaatctg ccattcatat gcatggctct
    14641 ttctaaattc acaaggcttc catgttttaa caggttaaga gccgttctgt tccatgtttc
    14701 attcatcaca tcaactataa aagattgtcg tgtgaaacaa gtaactgtaa ggcagatacc
    14761 attgactgct atgctgtcgc ctaaatgtat atcctctaat atttttgaag atttaatgga
    14821 tagtttacag ttataagtat ctttctgtat tctttctact ttaccgattt cttctattat
    14881 tcctgtaaac atggataaat cacttcactt tctatgagat aatcgtttcc cacttgagaa
    14941 aaagcatatg gtttgagcat aatggcgtct ttaggaaaaa caacaccttg accacttaca
    15001 gggctttttc cactaccccc aaatatttta ggtgctatat aagtttgtac ctcatcaaca
    15061 atctgttgct caagagcact ccaattcaac gcacttccac cctctaaaag cagactgtct
    15121 atctgcatat ttcctaaaca ttgcattaaa ttcattaagt caatatgatt accttttttc
    15181 ttcgtataaa taatttcaca accacatttt ctgtaaagag caattttact ttcatcaata
    15241 gaagaagtag cgatatatgt tttaatgtca ttcgctgttt ggataatttt tgatgtaatg
    15301 ggtgttctta aatctgtatc acatacgata cgaataggat ttttcccgtt ttctaatcgg
    15361 catgtcagta aagggttatc ttgaataacg gtatttactc ctaccatgat tgcagtaacc
    15421 ttatgtctga attggtgcac ttgttttctc gctttttcgc cacttatcca ttttgattga
    15481 tttgtataag ttgctatttt cccgtccatt gtcattgcat atttcataaa aacatacgga
    15541 atacgctttg taatatactt cctaaatact ttgattaact gttgacattc tatttctaaa
    15601 atgcctacat caacttgaat gttattttcc tcaagttgct ttacaccttt tccagacaca
    15661 atcggattac agtctaaagt tccgacgact acccttgtaa taccactttt aataattgtt
    15721 tctgtgcaag gtggtgtctt accatagtga cagcatggtt caagtgttac ataaagcgtt
    15781 gctcctacag gtgactctct gcaatttttt agtgcatttc tctctgcatg taatccacca
    15841 aaaacttcat ggtatccctg tcctataagc cgattatttt ttacaattac tgctccaacc
    15901 ataggattag gattgacata gccagcaccc ttttttgcca gttctattgc tattttcatg
    15961 tattctatat cgctcatttt tacacctcca aaaaaataca tgccttgaat aaaatctatc
    16021 caagacatgc aaaagaggat ttatgcgatt aaagaaaatg ctctgaaaca atataaaatc
    16081 attccagagc acgcaaaaaa gcataaactt acttttatct tctttcatcc agactatact
    16141 gtcggctttg gaattacacc aaatcatgcc tttcggctcg tgggctgtac caccggtagg
    16201 gaattacacc ctgccctgaa gatagtta
//
LOCUS       DAKBVG010000046        16219 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000046 DAKBVG010000000
VERSION     DAKBVG010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16219
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.046"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            <1..254
                     /locus_tag="OUD09_001041"
     CDS             <1..254
                     /locus_tag="OUD09_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCT9692323.1"
                     /translation="NKLKEYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCF
                     EELKSAIDSYIYYYNNERIKQKLNWQSLVQFRKTTVTVV"
     gene            443..1111
                     /locus_tag="OUD09_001042"
     CDS             443..1111
                     /locus_tag="OUD09_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HCT9692324.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1311..1697)
                     /locus_tag="OUD09_001043"
     CDS             complement(1311..1697)
                     /locus_tag="OUD09_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9692325.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1669..3051)
                     /locus_tag="OUD09_001044"
     CDS             complement(1669..3051)
                     /locus_tag="OUD09_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HCT9692326.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(3207..4394)
                     /locus_tag="OUD09_001045"
     CDS             complement(3207..4394)
                     /locus_tag="OUD09_001045"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCT9692327.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4401..5432)
                     /locus_tag="OUD09_001046"
     CDS             complement(4401..5432)
                     /locus_tag="OUD09_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HCT9692328.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5478..6902)
                     /locus_tag="OUD09_001047"
     CDS             complement(5478..6902)
                     /locus_tag="OUD09_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCT9692329.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMED"
     gene            7077..7982
                     /locus_tag="OUD09_001048"
     CDS             7077..7982
                     /locus_tag="OUD09_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCT9692330.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            8073..8750
                     /locus_tag="OUD09_001049"
     CDS             8073..8750
                     /locus_tag="OUD09_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCT9692331.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8747..8917
                     /locus_tag="OUD09_001050"
     CDS             8747..8917
                     /locus_tag="OUD09_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9692332.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            9031..9927
                     /locus_tag="OUD09_001051"
     CDS             9031..9927
                     /locus_tag="OUD09_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCT9692333.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9982..11352)
                     /locus_tag="OUD09_001052"
     CDS             complement(9982..11352)
                     /locus_tag="OUD09_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCT9692334.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11590..12336
                     /locus_tag="OUD09_001053"
     CDS             11590..12336
                     /locus_tag="OUD09_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HCT9692335.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12333..13172
                     /locus_tag="OUD09_001054"
     CDS             12333..13172
                     /locus_tag="OUD09_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCT9692336.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            13173..14123
                     /locus_tag="OUD09_001055"
     CDS             13173..14123
                     /locus_tag="OUD09_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HCT9692337.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14264..15016
                     /locus_tag="OUD09_001056"
     CDS             14264..15016
                     /locus_tag="OUD09_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9692338.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            15035..16186
                     /gene="queG"
                     /locus_tag="OUD09_001057"
     CDS             15035..16186
                     /gene="queG"
                     /locus_tag="OUD09_001057"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCT9692339.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5278 a         3031 c         3262 g         4648 t
ORIGIN      
        1 gaaacaaatt gaaggagtac aaaatctttc aaagcatgtc tcggaaaggg aactgcttag
       61 ataattcact tatggagaat ttttttggct tattaaaaca agagattttt cacgggaaag
      121 tttataactg tttcgaggaa ttaaaatcag ccatcgatag ctatatctat tactacaata
      181 atgaacggat aaaacagaaa ttaaactggc aaagtctcgt ccaatttcgt aaaacaaccg
      241 taacggttgt gtaaaaatat cattttagag cataaaaaaa aggagtggat ttctccactc
      301 caaaaaagtc taacttttgg gggtcactac agtgtcacag tccttttttt agtaaaagga
      361 tacatttctt ccttgtatat tccatgcgtg ttcgtgagaa agcatatata atttaattaa
      421 cagatagtta gggggagaaa aaatgatttt ttcaattaca atgaaactgg tgttcgggtt
      481 gatcggttta ttattagtgg tacgtttgct tgggaagaaa tccatgtcag aaatcacacc
      541 gtttgattta gtctatacgc ttgttttagg tgggatctta gaagaatcaa cctatgatga
      601 caatgtccat gtagggcatg tcctatttgc cattgcgctt tgggcagtaa tgatttatgg
      661 tatcgaacgt atcgtccaaa aaaatgaaaa ggtcaatcgt tgggtcaaag gcgagccatc
      721 tgttctaatc aaagacggcg tcatcaacat gacagagttg acaaataacc atatcgagat
      781 ggagcagttg cgagcaatct taagacagca agaatgcttt tttcttgaaa atgccaaaca
      841 tgttatccta gagaatgccg gtcaaatgag tgtattgaaa aaatcagatg aagacaaagc
      901 tttatcgatc ctgcttgtag atgaagggca aattcagcat aaagtgcttc agtccaatca
      961 attgactgaa gcatggctta tggagaactt gaagaaagaa ggctatgcag atgtaaaaca
     1021 gctgatttat gttgaatggt cagaagaaaa agggctctac atactaaata aagaagacgt
     1081 catgcatgaa tcttaccgaa tagatggata gatagacaga ggttgtgaaa tcagcgcctt
     1141 ggcttgttac tcaaaactaa gcgacttttt cacaacctct gatatctaat atacgttgtt
     1201 ccagaagttg cttcttcgaa aaataagcca aaattccaca aaaatttaag aagcaatttt
     1261 cgaaaaatcc tttcttattt tttgaagttg atcacttctg tcacaacctc tcatgtcaca
     1321 aaccattttt ttgtttcctt taactcaata taccgtacgt cttcccacaa tatcggctga
     1381 tggtcaatca taaagtaatc ttcataggct tcaccaatga agtgcccttc gacctcatcc
     1441 aagagtcttc caaattcatc ttttaaattg agctgaatga ttactgcttt acgagataaa
     1501 aaggcttctg tcagtatact atcgatttct tgcaatgtca tttgttgttt aggaagaggc
     1561 tgtttgcgtg cttcttcttc attttttttg ataccgttga ctagttcttc catcgcaaaa
     1621 gcagtccccc attttaatcc aaatggccga tctcgatact cgttatagtc aatgaatttt
     1681 tttgctgtcc gtctcatctt ttccaccttc taatccactc ataccacctg catgcccgcc
     1741 aacgagagaa ctacgtgcga tggcgcgtcc tccttgcaag agactatgcg catggatgat
     1801 tgattgaaag ccgtattttt ttctaatcgt atcgacaaca aaatcaactt tcgcttcatt
     1861 tatctgcttt tcaggatctt caaaaaggtt aagctgtaaa cctgtcgcgt atacgaggtc
     1921 ggaacagttc acgcctacac gccgaatatc ctggtagtgg taatgctggt ggaaaatggt
     1981 caataaagca tctgctagat ctctgctagc atttgtcgct tcgattttca tttgctggtg
     2041 aaacccggtc ttgccatttg gatcaagata gcctaaagag tatcctaccc ataagctgac
     2101 tacttgtgtt tttgctgcag atcgtctcaa gcgagtgccg acttggtcgg ccatttcttt
     2161 gataacgatc tcgatttctt ctcgtcttgt ataatctcta tctaggattt gactatttcc
     2221 gatacttttt gattttggtg tatattcttg tcccaaaaaa cttcgatcga tgccccaagc
     2281 atgggcataa agctgagtac cgatgacccc tagcttgctt ttcaagtgat aataatccgc
     2341 gtgagctaaa tcataaacag aatgtatacc catttgtttc agacgttttg ctgttcgttt
     2401 gccgattccc caaaagtcag tgatatctga gattttccat aacttttctg gtacatcttc
     2461 ataacgccat tcagcaaaca tttgctttcc gtcttttgag ccattgtcta aggctagttt
     2521 agcaagtagc gggttatcgc caattcctat agtcgtgtag attccggtct gctcaaacac
     2581 atctttatgg atcagtttag ccatgtcgta cgcatttgtt ttaccaaaga gtgccatgct
     2641 atttgttaca tcaaggaagc tttcatcgac ggaatagaca tgatggttcg cttcgtctgc
     2701 atatttttta tagatagaat tgatttctct gttcttttcc atataatagg ccatccttgg
     2761 ggcaacaata tataaatcac ttggataggg gaagggaaga tctctggcac ggctgatatt
     2821 ggtgatgccg taagcttttt ttgctgcagg actggaagcc aagattaatc cgctaccccg
     2881 tgtttctgtc gaatcggacg gataggacat gactactaat ttagttgtaa ggggatcaag
     2941 atccttttca acgcactcta cggaggcgta aaaagatttg cagtcaatac ataaaatatc
     3001 tcttgaaggt tctttttcat aaacaaatac cggattttga ttcgcccaca taacaatcac
     3061 ctcttcaaca caattagaac acacgttctc tttccgtgtc aataaaaaac agcaattttc
     3121 aaatacgagc aaaaggctca catttggaaa ttgctgagag gttattttta tctatttgtt
     3181 tttagctgag atcgttgatt tttttatcaa acatgaacag cttctggatc gatctttgtt
     3241 gtcccatttc caatgtgtgc aacataaaat tccggagcat agccgacaac tttcatgtat
     3301 tcatcataga cattgttttt gaaggcaggt atattttctt ctttgacaag agcaatcgca
     3361 cagccaccaa atccagctcc tgtcattcgt gcacctaaga caccttcttg tttttgagca
     3421 gcatctacta gtgtatctaa ttcgatccca gttacttcat aatcatagcg aagagagtgg
     3481 tgagaatcat ttaacagttg accaaatgct tctaagttcc cagcttctaa ttcagctttt
     3541 gccttcaaag tccgctgatt ttcagaaacg gcatggcgag ctctgcgtat caatgtatca
     3601 tcgccgattt ggtcaatagt tgattcaaat tcttcttcat ctaagtctcc taaagaagag
     3661 ataggcaaag ttttttgcaa acgtgccaag gcttcttcgc attcgctacg gcgttcattg
     3721 tattttgaat ctgccaattc ccgccgctta ttcgtattca tgatgacgat tgcataacca
     3781 tctagtttca ctggaaccat ttcgtatttc agcgtatttg tatctaataa gattgcatga
     3841 tctagctcac cgaagccgat ggcaaactga tccatgatac ctgaattgac accgatgaat
     3901 tcattttcta cttttttacc tgtttggact agttccaagc gatctgtttt taaatcaaac
     3961 agatcttcta atacaacacc tactaataat tctaatgaag cgctactgga aagtcctgca
     4021 ccattaggga ttgttccttc cactaataac tcaaacccat gatcgattga gtgtccagct
     4081 tcttttaatt taaggaccat tccttttacg taatttgccc agtttgcttt tttatcatat
     4141 tctagctcat ctaaagtaaa actaatgaca ccgatatcgt caaagtttgt ggaataaact
     4201 aatattttat tatcgtctcg aggtgctgct acaccttgcg ttccataagt gatggaagcg
     4261 gggaagacat ggcctccatt gtaatctgtg tgttcaccaa tcaaattgat gcgtcctggt
     4321 gagaagtatt gtgtagtttt tttaggtccg aatgtttcgg caaattttgt gtttaagctt
     4381 cttttgattt ccatgatgag ttattccttt cttgttttga ttttgaattc tgtcactgtt
     4441 tcaaatactg atccggcttt taaatgaatc gaaccaaatg ctgagaagcg ctctgcacct
     4501 ggagctgttt gagtctcaaa tgtgatgcca ccgtgatgtg ccagttttcg gttgcgcatt
     4561 tctggtgttt ctgtgccgaa atttgcagtg aaaataacca cactagaagc atctgtagca
     4621 atatctactt ggattctttt atcggggcta gtcaagcgag cggcttcttt acccaagccg
     4681 gtttctttta agaaaaatgg atgatcgatt ccgtcaacaa gatttttttg atccaaatca
     4741 ctagcaaaaa cggacgctaa ttttttaggt atttgaaaat cgaaagcact tcctgcagca
     4801 ttttctttta cccctatagg aatactatct gttcgcaaag gagcataagc ttcgctattg
     4861 agccaaagct catgttgatc gattgactga gaagcatctc ccgtcaagtt gaagtaaaca
     4921 tgatttgtcg gattgaataa agtatcttgg tcactggtgc cgcgagaggt cactcgccag
     4981 atattatctt ttgtcaaagt atagcggatt tccacatcca ttgtacctgg aaaaccatgt
     5041 tctccgtcgg gacttgtagt agtaaaaatg acagaagctt cattttcgcc attcaaaatg
     5101 acataagacc atttttttaa ttcaaatcca ggagcaccac catgcagatt atgtccagtt
     5161 tttggatctg tggctaattg gtaagttttt ccattgagag aaaagcggcc attttcgatt
     5221 ctccctgcgg tgcgaccgat agaagcaccg atatacggat ctttttcgat atattcttca
     5281 gcggtgtcaa atcccaaaac taattctcga tcatttgatt ttaaactgac gattctagca
     5341 cctaaatcag agatagaaag tgctagcttg tttttgttct ccaaggttat caagtgataa
     5401 ccttggccaa aatctttttc tgtaatcttc attttcaaga tcctttctta atgggaagga
     5461 actactgcat cgtgaagtta atcttccatt gtatcagctg gagccaattt gttttccaat
     5521 ttctcaacga tttgtgcatg ttttttctct gtcaatgtaa tcttccaagc aaaaataata
     5581 gcggagaaaa tcatcaaagc agcaggagca tagaatgcat aagctttgaa agtcgttacg
     5641 ccatgagcag tgatatcagc agcagtagca ttaccagtca tcccagaagc gatagcgaca
     5701 aaccctacga tcccgttaga caaagcacca gcgattttat ctagtaaagg gcgaattgat
     5761 agagtaacag cttcgttacg gataccattt ttccattgac catattcaac agagtctgtg
     5821 atcgtcatca aagcggataa gaaaatcaat tgttgaggga agtagaacaa tactagcccg
     5881 atgataacga ttggcaaact tgaacctgcg atcgtaaaga aaaagtaaga agaaatcatc
     5941 attgcgatcc ctccaaggta aacgtagcga cgagagatga cgcgtgtaag cattgggaat
     6001 agtggaacag ctactaaacc aagtaaggca gcaactagac caacgatcca aaattcagtt
     6061 ggttggccaa gtacatattt gaagaagtag aataatacgg cagttgttgc tgcattggca
     6121 atcgcataag cgatataagc aagggcaagc cacatcaact gatcattttg agcaactgct
     6181 ttgaacacat ctttgatccc agctttttca ttttgctgac ggatcgcagt atcgacttct
     6241 tttgtgccca aggcagtgat aatcgcagta ccgcctgaaa taacagcagc aatcacaccg
     6301 aaccagaacc agcctgaagc gccttcaata tgtttatgag taacaagata ggtaaagaaa
     6361 gtaacgatag ggacaacgac tagagtagta ccatttgcac ctagagaaga gccgaaacga
     6421 gcaaacgtag caaatttttc acgttccttt gtttccgtac tcaaagctgg aatcattgac
     6481 caaaaagcga tatccttaaa tgaataaaaa ctatccaata agatgaaaac gattgcaaat
     6541 aatatgataa ataaagtcgt attatgagta gctaagccaa agaaattggt aaagattgct
     6601 accaagagta cggaactaac aacgccgccg ataactaacc atggtttgaa tttaccgtat
     6661 tttgtacgag tattatcgat gatgccacca atcaaaggat caaaaataat ttcaactaag
     6721 cggataccta caactaagct tgtaactgta gcaatcattt ttgcattcgt tgccttatct
     6781 gttcctgcga acatctggct tgtcacgaat accataaaat aagtgcttaa tgtaacatag
     6841 aatgtgtcgt gtccaaaagc gcctaaagcg taggacacac gttggatcat tttgcttttc
     6901 ataatctgta ttcctctcct gttcaatata taatactaag taaaccggtt acaacgaata
     6961 acaaagatct tttaatgact taaacttaac aagaaatcgc ttccttttta atatatttct
     7021 gtgactttta tttaactttt tgttgcatta tactaaagga gtaaggaggg atagacgtgg
     7081 agcaatcgat ttttttaaaa aaggaacatc aacaagtaga aaaagaattg tattttgatt
     7141 tttgtggttt ctctaaaacg atgccgtttc attcttttgg tcctgctatt cgcaatgatt
     7201 acatcctgca tgtcgtgatg gaaggcaaag gatcttatca tgtgaaaaat caacaattcc
     7261 aattgaaaaa aggtgatctg ttcttgattc gtccaggaga ctcgacgttt tacttatcag
     7321 atggagaaga tccgtggatg tattgctggc tttcatttgg tgggagtgct gcagaagaaa
     7381 tcattcggca gtcgcttttc agagaagacc aatacacgat ggtctcttct gacatacaaa
     7441 aatatatcga cattatttta gaatgcatga aagatacgga agaaaccttg gaaaatgaat
     7501 tacagttaac agcattaacc taccgttttt tatcagtcct tttaaaagat ggcggaaagg
     7561 tcagcggagg tgtacaaaag gttcattcct ctttagctgt tgaaacagtc aaatacatcg
     7621 aggcacatta tgctgaaaaa ttgtctgttg aggaaatcgc ccagcattta gcggtcaatc
     7681 ggagccattt gtcgcgagta ttcaaaaatc atatgggcac cagtatcaag gaatatttga
     7741 tcggggtacg gatcaatcga ggggcgtttc ttctttctct gacggatgaa tccgtggaaa
     7801 gtattgccta tcaagtaggg ttcaatagtc tagtcgtatt tagccgaatg ttcaaaaaga
     7861 gtaccggaga aacggctacg aactatcgta aacgtatgaa aaacgaaaat tttaagaata
     7921 ttgctttgga taccttgaaa aaacagttgg aaaaacaaac cgcagtttct tggacaacct
     7981 gattagtctg gttaaaatga caagcatcgg caaaagaaac ttgtggcggt tatgctacac
     8041 tgtaaagcga agactaaaaa ggaggaaaag ggatgcgtac acttatcatc attagccatc
     8101 caacgatcga agaatcttta aatcagcaat ttttcaaaga agcttctgct ggtttaccag
     8161 ctacttggca tcatttggaa tcaagttatc cagacggaaa aatagatata gagactgaac
     8221 tacagctctt ggcccagcat gatcggatca ttttccagtt tccgttttac tggtatagtt
     8281 ctcctgctca tttaaaaatt tggcaagata atgtcttgga acgagcagca catgtattaa
     8341 aagggaaaga actaggaatc gtattgacaa caggtgtggc ggaaaaagaa taccaagctg
     8401 gaggaaaaga agaatatacg atttccgaat ttttgaggcc gtatcagcga atcgcaaata
     8461 aattccatat gacctatctt ccgccattcg ttttagcaca atttatgtat ttatcccagg
     8521 aaaaacgttg ggagaagcta attgcttatc agcagtatct ctcattggaa ggcaaaccat
     8581 cgctgactca gcggattgac tggtttattc aaagagtaca ggaaaatcag aaaatgcagg
     8641 aagaagaatc tgaaaaacaa gcatatatcg tagaagcact gactgaggca aaagagcaga
     8701 tcgaagactt gacctttaca ctgcaagaaa tgaaaggaac ttcattatga caaatgattg
     8761 gctgatccaa caattagatg aattaagcga aaaatcagaa tatcaagacc gtgcgctctt
     8821 atatgaaata aagaaactga tggctgagat agacgaacga acaatgcagc tgcaaggaga
     8881 aatcgacgga caatcttgga atcatctcaa ctggtgaaat tttcttataa ataacaaaga
     8941 taaaaatgtg cacagcgaca attatttgct acaataagaa tatgaagcgt ttgataacgc
     9001 tattatacat aaaaattggt ggtgaaaaaa atgtatgatg aaaatgagtg gggagaaaat
     9061 gttcggatga cccgaagaag acagcaaaaa caggcagctc gtaagcggat gatcatgatt
     9121 ggggcagcag ttgccgtgtt tttgatcata gcaggaggag ttgcgggtct atattttcat
     9181 ggaaaaaatt cgtcagaagc gaaaagtacg accataacgg aaacaacgac aaagaaaaca
     9241 gagacgaaac aaacaagtga gtcatcaact aaaacaaaag agacacaaac ttctgaagaa
     9301 aaaacagcaa atggtttgcc agagcaactt caagcaatcg taaatgatta ttcgaaaaag
     9361 atcgaagaaa agacgcctgc attgattgaa gaataccaga cagaaattca gggaaatcaa
     9421 gagggaatag ctggtttatc agcagttgct aaccaaaaag cacgtgagct tcaagcaatc
     9481 tccgacgaag ggatcagaaa attacgtgca gcgtatcagg cagcagaaaa taaagacggt
     9541 gtagacttag atactttgat caatcaatta tcggctaatt atacgaatca tgtcgctaag
     9601 atttccgata tttatttgca gactagcgcc tctcttcaag cagagtcaac aagcacacaa
     9661 gacacaacat cttctgattc tgagacagca gagtcggtag aagcttctga aatgcaagct
     9721 cgaacaacac aagacacaac agattcctct gaaactaata ccaacagcgc gacttcacag
     9781 gctgcgacaa ccgtagtttt ggaaggagaa ggtccaaatc aaatagccga acgaactggc
     9841 gtaccagtag aaacgatctt gtcgctgaat gggatgagca tggagaatta tttcctgact
     9901 cccggtgaga cgctaaaatt gaattgataa aagcaataaa aaagaacgaa gcttccacta
     9961 aggtgaagct tcgttctttt tttattttcc ggttcttttt tcgcgtacaa agtaatggat
    10021 cgtcatgtaa agcaagaaaa cgagtccaat aatcaatgag attctcgttt caggattgat
    10081 aaacatgaaa atcaatacga tagctaaaaa agcaagtgtc acataattcg tataaggaga
    10141 tagtggcatt ttaaaaggat gatcatgcat tttatctgcg tgcttctttt tgaagcgaat
    10201 ctcgctgatc aaaatcacta cccaaggaac cataccaggc aaaatacttg agctgtatac
    10261 gaaaacgaaa atattttctg actggttgat gaacattggt agaagataat ttaagatcag
    10321 accaattaag attccacctg agattgctac gacaggataa aaaggcgcac cgtgacgaga
    10381 tagtttcaga aattttttag acatctttcc atttatggca agtgtataga ccatacgact
    10441 tgcgctgaaa atccctgaat tgcaacctga taatgccgct gtaatcacga caaaattaat
    10501 cagtcctgca gcaaatgtaa tgccgacttt agaaaaagtt tgtacaaaag gtgaaccaat
    10561 ctcactcaat tgattccacg gatagatact cacaatgata aaaatagcac caatataaaa
    10621 aatcaagatt cgaccgatag tggactggac agcttcaatg atcgtctttt taggattctc
    10681 tgcttcacca gcagtcattc cgatcagttc gattccttga taggaagcaa caacgatcga
    10741 taatgcaaag aaaaatcctt tgacgcctcc agtgaagaat ccaccatttt tccaaagatt
    10801 tgaaatccct actgcctgac catgattacc aaatccaaat aagattaccc caagacctgc
    10861 aatgatcatc aaaatgatcg ttatgacttt gatgatcgaa aaccaaaatt ctaattcgcc
    10921 aaacgccttg acagaaataa gatttgccag cataagaaac aagacgacaa tgattccggg
    10981 aataattgcc ggcaaatcag gccaccaata attcatataa gatcctacag caatcacttc
    11041 acttattcct acgacaatat attgaaaaat attactccaa gcggtcaagt agccagccac
    11101 aggatggatg tactcgctcg cgtaatttgc aaaagaacca gtcgaaggat caacgtaaag
    11161 catttcgcct aaagcacgca taatcaaata caaaaagaat cctgcaatcg cataggctcc
    11221 catcactgag ggccctgtcc aacgtatggt tgaggaggca cccataaaca gaccgacacc
    11281 gatcgttcca cctagtgcga tcatttggat atgacgggca ctaagacttc gagttaactt
    11341 ttcttcttcc atacaaaaac tcctttcgtt gttgagtcaa caagaaaaaa taatcaaacg
    11401 tttcaacaca tgtttttcat tattaggtta gccttattga actcttgatg tgattctaca
    11461 gaaaattttc ataaaatgcg aggaaaattc gaaaaaaact ttttcatcat tctacttgaa
    11521 aatgctttcc attttagtta aactatattt aaataaaaag ttaggtaaac ctaaaaagga
    11581 gtgtaagatt tggaacgtac ggccattgta attcaagacc tttcagccgc ttatcaaggg
    11641 aaaactgttt tgaaaactat caacttgacg ataaatccac aaaaaattac cggtattatc
    11701 ggaccaaacg gtgcaggaaa atcaacattt atgaaatctt tgctagagtt gataccagct
    11761 actactggta aagtcacctt ttctggacag ccggtaaact cagtccgtaa aaaaatagcc
    11821 tacgtggaac agcgaagtga attggatctt tcctttccaa ttgatgtatt aggcgttgta
    11881 cttttaggaa catatccatc tttacgaatt ggacaaagac ctgggaaaaa agaaaaagaa
    11941 cgtgcaagac aagctttgaa aaaagtaggg atggaagaat atgccaaaag acagatcagc
    12001 gaactatcgg gtggacagct tcagagagtt tttattgcaa gagctctagc ccaaggagca
    12061 gaatggatct ttttagatga accattcgta gggattgatg cgttaagtga acgaaagatc
    12121 tttgacatct tgcaggaatt gaagaattca ggaaaaacga ttttgatcgt ccatcatgat
    12181 cttcataaag tagaagaata tttcgatgag gttattcttt taaataaaca gctgatcgct
    12241 tccggtccag tacaagaagt ctttacatcg aagaaccttc aattggccta tggtgagatc
    12301 atcagacatt tagtaaaagg aggagagaaa aaatgatcca atcctttatt gatggattaa
    12361 tgaattatca attcctgcaa tacgctttgg tcacttccat gcttgtagga cttgcttctg
    12421 gagtcatcgg ttcttttatt attttacgag ggatgtcttt gatgggagac gctatctctc
    12481 acgcagttct tccaggtgta gccatttctt atatgtttgg ttttagttac atctttggag
    12541 ccactgcttt tgggatgcta acagcagcag tgattggttt cgtgactcag cggagccgat
    12601 tgaaaaatga tacagcgatt ggaattgtct ttagctcctt ttttgcatta gggattatct
    12661 tgatttcata tgcccagagt gcgacagatt tgtatcatat tttatttggg aatgtcttag
    12721 ctgttaggga aagcgatttg ttgctgacag cactcgttag tggaatcgtc ttgatatttg
    12781 tctttttctt ttataaagaa ttgaagatca catcattcga tccgacaatg gcaaaagctt
    12841 atgggctgaa cacatcgctg atccattatc ttttgatgtt ctttttgaca ttagtggctg
    12901 tagtcagttt acagacagta ggaacaatct tggtgattgc catgttgatc acaccagccg
    12961 ccacggctta cttgctaacg aaccatttac tgaaaatgat cattacagct gcaggaatcg
    13021 gtatgctaag tgcagttgtc ggtgtgtttt tcagttatag ttacaattgg ccatcaggag
    13081 ctacgatcgt gttagcatgt accgcatttt ttatccttgc ttttttattt tctccaacaa
    13141 aaggaatttt atttcagaaa ggaagcagat agatgaaaac aaaaaaatca ctctttttaa
    13201 tcttggcagt tagtttcttg gtattagccg gttgcggaaa gcaggcttct gaccaagcgg
    13261 atgaaggaag taaagaaaag ttgtcagtcg tggctaccaa ttcgatcttg gcggacatgg
    13321 caaaagaggt cggaacagat caaatagata tccacagtat cgtaccagtc ggaacagatc
    13381 cgcatgaata tgaagtacta ccagaagaca tcaaaaaggc aagcgatgca gatgttattt
    13441 tatacaacgg tttgaatctt gaaacaggta acagctggtt cgataacttg atggaaacgg
    13501 ctaaaaaaga agaaggaaaa gattattttg cagttagcaa aaatgtagaa cctctttatt
    13561 taactagcgg tgaagaacat acaaaagcag atccccatgc ttggctagac ctatctaacg
    13621 gaataaaata tgtggaggaa atcgcacgta tattctctga aaaagatgca gaaaatgcga
    13681 cactctataa aaaaaatgca gaagcatatg tggaaaaact aaaagaatta gatacacaag
    13741 cgaaggaaag ttttgcttct atcgaagaga acaaaaaatt attagtaaca agtgaagggg
    13801 cattcaagta tttttcacga gcatatgatt tgccagcagc ttatatatgg gagatcaata
    13861 cagaaagtca aggtacgcct gatcaaatga aagcgatcat tgatcagata agagcaaaag
    13921 aagtaccggt tttattcgtg gaaaccagtg tagactcaag aagcatggaa cgggtagcaa
    13981 aagaaacggg tttgaaaatc tatgataaac tgttcactga ttccatagca aaagaaggag
    14041 aacaaggaga ttcttactat cagatgatga aatggaatat tgaaacgatc catgaaggtt
    14101 tgtcacaaac aaaagaaagc tgaaaagtgc aaggtctagc ttgaggagca gaccttgcat
    14161 tttttataag agatatgaaa aaaaattatg ctgtttttcg tcgtacagat accttttttg
    14221 aagaaattcc gttatagtta gagtaaggaa ggaaggtgtg gctatgaatt ttacacgtaa
    14281 agataaatat acgattaaag aagaagtctt tcgattgatc agccagcagg aaaataccga
    14341 caaggatcaa gaccttcaat ttcttgaagc agaattagct aaattattca tagcaaatca
    14401 aaatgagaca acagaaccag atgaagagct gtacataggc aaatggttgt ttgatgaatt
    14461 tcagccttta gatgaaaata ccggtatcgg ttattttgtg ttcctttctc cagtcggctt
    14521 cacacctggc gtattagtta aacaaaacga cgaaataacc gtcaaagcat ggcctgcatt
    14581 attttcagtc atcaaagcag aaggagtaat caaaaagatc cagtcggcct attataaaag
    14641 tcaatttatt gccaacagca aattaatgaa tatgttttat aatcgtctag tagaaattgc
    14701 taggcctggg aaaatcccgc aaacattcac gtacacacag caaaaaaatc tctatgaaga
    14761 agtattgtat tcccgcgatc tgtctgagtt ttcgccatgg gaagaggtaa ggctccatga
    14821 actgttaagt acaaaaccag tttcattgaa tttttttaat gcattagata acaaaagaaa
    14881 attaatttca acaaattatt gggaagtatt tgctgcaaga aaaaaatgga tggaagaaaa
    14941 gaagattgcc gatcagcaga tccagtttgt ccagctgaaa aaagaaaaac tatattatgg
    15001 gttatacagc cgttaacagc aagcgaggaa gaccatgaca ttaaaagaag aaatcataca
    15061 agaaagcaaa cgattaggaa ttgacaaaat cgggttcacc acggcggaac cttttgattc
    15121 attaaaagaa tcgttggaag aacaaaaagc agctggccat acttctggct ttgaacatcc
    15181 aaatatcgat gagcgtcttt atccagaaat gacatttgat cagccgaaat cgatcatttc
    15241 cattgcactt gcgtatccta ctaggatcca tgaagaagtg cctagggatg aaaaacgcgg
    15301 gcagtttgct cgtgcttctt gggggatcga ttaccatgat atactcagag accgtttgaa
    15361 ccgattgatc gaattcatcc aaacaagagc agaaaaatgg caaaaagaag aggaatggcg
    15421 tctagctccg caagtagata ctggagaact gatcgatgtc gctgtagcac agcgagctgg
    15481 tttaggattt attggacgaa acggattgtt gatcactgaa gagttcggtt catttgtcta
    15541 tcttggtgag atcgttacta atattgtatt tgagcctgat aaaccaggag aatttggttg
    15601 cggtgactgt atgcgctgcg tgactgcttg tccgacaaaa gcattgcttg gtgacggaag
    15661 aatgaatgcc cagcgatgtt tgtcttatca aactcagaca aaagggatga tgccggaaga
    15721 atatcgtaaa aaaatgcgaa atgtcattta tggatgtgat atctgccagt tagtctgccc
    15781 ttacaatcaa ggaaaagatt ttcattttca tgaagagatg gaaccaaaag tagacgaaat
    15841 atatccaaaa ttaaagccaa tgctttcgat gtctaataaa gacttcaaac agcaatttgg
    15901 gcatttagct ggttcatggc gtgggaagaa acctttgcag agaaacgcat tgattgcttt
    15961 agctaatcta ggtgatcgaa cggcattgcc tgagatttca gaatgtttaa cagatgtccg
    16021 ccctgtcatc agaggaacag cagcttgggc tattggacgt ctaggaacaa aagaaccgga
    16081 aaaatggctt gatgtattag atgaaatatt agctaaagaa acagaagaag tcgttataaa
    16141 cgaattggaa catgcccaga aattattgcg gagaaagtta aaataaaaaa atagttaaca
    16201 ttattcaaag tttttgtcc
//
LOCUS       DAKBVG010000047        15964 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000047 DAKBVG010000000
VERSION     DAKBVG010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 15964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15964
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.047"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            249..2015
                     /locus_tag="OUD09_001058"
     CDS             249..2015
                     /locus_tag="OUD09_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HCT9692340.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPVHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2117..3376
                     /locus_tag="OUD09_001059"
     CDS             2117..3376
                     /locus_tag="OUD09_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCT9692341.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3523..4824
                     /locus_tag="OUD09_001060"
     CDS             3523..4824
                     /locus_tag="OUD09_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCT9692342.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            4827..5678
                     /locus_tag="OUD09_001061"
     CDS             4827..5678
                     /locus_tag="OUD09_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCT9692343.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            5691..6506
                     /locus_tag="OUD09_001062"
     CDS             5691..6506
                     /locus_tag="OUD09_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HCT9692344.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            6657..7823
                     /locus_tag="OUD09_001063"
     CDS             6657..7823
                     /locus_tag="OUD09_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCT9692345.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            8053..8700
                     /locus_tag="OUD09_001064"
     CDS             8053..8700
                     /locus_tag="OUD09_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HCT9692346.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            8784..9728
                     /locus_tag="OUD09_001065"
     CDS             8784..9728
                     /locus_tag="OUD09_001065"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HCT9692347.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTIQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            9863..11314
                     /locus_tag="OUD09_001066"
     CDS             9863..11314
                     /locus_tag="OUD09_001066"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HCT9692348.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            11335..12171
                     /locus_tag="OUD09_001067"
     CDS             11335..12171
                     /locus_tag="OUD09_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HCT9692349.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            12171..12830
                     /locus_tag="OUD09_001068"
     CDS             12171..12830
                     /locus_tag="OUD09_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCT9692350.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            12956..13645
                     /locus_tag="OUD09_001069"
     CDS             12956..13645
                     /locus_tag="OUD09_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9692351.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            13651..14607
                     /locus_tag="OUD09_001070"
     CDS             13651..14607
                     /locus_tag="OUD09_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HCT9692352.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            14651..15163
                     /locus_tag="OUD09_001071"
     CDS             14651..15163
                     /locus_tag="OUD09_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCT9692353.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(15419..>15964)
                     /locus_tag="OUD09_001072"
     CDS             complement(15419..>15964)
                     /locus_tag="OUD09_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCT9692354.1"
                     /translation="NYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQE
                     DYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWHL
                     YEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSLL
                     VVSNDGAFQEAADKIKKGLLI"
BASE COUNT         5218 a         2813 c         3247 g         4686 t
ORIGIN      
        1 aaaactgtta aaaaactttt actttagaaa attggagaaa ttgatcgctt aagataaatt
       61 caggacacta aatagtacag gaagtagact aaaactttta aaagttttaa gtctatctat
      121 ttggcaagtt taaaaaataa aaattcggta tcaaaaaact gttaccgata acatttttga
      181 aagcgttgtc ttttgcttct ctatcactta tagtgatgac gtcatattaa aacacaagga
      241 ggaaatcaat ggatacagct gcaatttatc atcgaccgga aagtgaatat gcatttctat
      301 atacgaatac aagatttcgt attcggcttc gtactaggaa aaatgatatc aaaaaagtat
      361 atgtgctatg tggagatcca tataccataa caactgaaaa atggtatcaa aaacaacgac
      421 caatgaaaaa atgtctaagt accaatgttc atgattattg ggaaattgaa gtgacgagcg
      481 aaacaagtcg cttacagtat gcgttccatg ttgttgggga agataacacg gactgttttt
      541 acggtgatca aggaattttt ccttatcgag aaaatgtgat tactgaacca aatttttatt
      601 ttcgaatacc ttattttcat caaatcgatc gattcactat tccagaatgg gtaaaagaaa
      661 cagtatggta tcaaattttt cccgaaagat ttgcaaacgg ggacaaagca aatgaccccg
      721 tacacacttt accttgggga agtaaagatc cagaccgaga agactttttc gggggagatt
      781 tgcaaggtgt gatggatcat ttggattact tgacggacct tggcatcaat ggcatctatt
      841 tttgtcccat atttaaagcc acatccaatc ataaatacga tacgattgat tattatgagg
      901 tggatcccgc ttttggcgat aaaaaacttt taaaaaactt gattgatgaa gcacataaaa
      961 gagggatacg tatcatgttc gatgctgtat tcaatcatat gggtgtccat tcaccacagt
     1021 ggcaagacgt tctcaaaaac ggagaaaaat cgatctataa agactggttc cacatccgtt
     1081 ttttcccagt agattcttat cagatgactg acttaccaga aactgctgaa aatatcccat
     1141 atgatacttt tgcttttact ccattcatgc cgaaactaaa tacagcaaat ccagaagtgc
     1201 aaaaatattt gctggatatc gcaacttatt ggataaaaga attcgatatt gatggatggc
     1261 gtcttgatgt tgctaatgaa gtcgatcatc atttttggaa aaaattcaga gaagcagtta
     1321 cagaaatcaa accagacatc tatatccttg gagagatttg gcattcctct caagcttggc
     1381 tgcaagggga tgaattccat gcagtgatga attatgcatt tacagattcg attaaagatt
     1441 attttgcaaa aaagaaaatc acagcttcac aaatggtcag tggaatgaat catcagcaaa
     1501 tgctttacag agatcaggtg aacgaaggaa cattcaactt attagattct catgatactg
     1561 ccaggatctt gaccctttgc caaggtaaca aagaattgat gaagtctgtg atggccttta
     1621 tgtttcttca aaaagggtct ccttgtatct attacgggac agaaataggg atgactggtg
     1681 aagatgaccc ggattgccgc aaatgtatga tatgggagaa ggaggaacaa gatctagagc
     1741 tattcggttt tataaaagaa cttgtttctc taaggaaaca gttgtctaag attatttctg
     1801 aaggttctac tcagtggcta atagtaaatg atcgtgaaga taagctctat ttcacacgga
     1861 aactagaggg acagatcatc tatgtttact ttaatcagtc aaaagaacct tgggtagtag
     1921 aacaggaaaa tgaggtgatt cttagtcaaa attgtcagtt gttagaagac gggaaagcag
     1981 agatacgaca atacggattt ttgatcatgt gttgaaaact gttaccggta acaacaagaa
     2041 agcgtttgca aatgaagatt tgtgcccatt ataataaggg tgtaacagca cgttgaatac
     2101 aaaggaggaa atgaagatga aaaaaagaag tgtgaaacag ttgggtttag ggttaatctc
     2161 tttaggaatc ttaggcggat tagcggcttg tggcaatggt aactcttcag cagaagggga
     2221 agccgatgaa ggaaaaacgt taacagtttc cgttgattct ggatataaag attatataaa
     2281 cgaaataaaa aacactttcg aaaaagaaaa cgatgtgaaa atcaagctag tagaaaagga
     2341 catgttcgat cagttggaat cattagcgtt agatgggcct gctggaaagg gacctgacgt
     2401 tatgatggca gcatatgacc ggatcggtgc gttaggacag caaggacatc tggcggaagt
     2461 aaaactggga aatgaagcag cttacgatga aacagacaaa gcccaagtga cttatgatgg
     2521 aaaaatctac ggagaacctg cagtaatcga gacccttgtt ttgtactata acaaagattt
     2581 agtcgataca gcaccatcaa cgtttaaaga tttggagaat ctatccaaag acagtcgttt
     2641 tgcttttgag tcagaagcag gaaaaaatac aggattttta gctaaatgga ctgactttta
     2701 ttattcctat ggtttaattg caggttacgg tggctatgta tttggtgatg atggcacaaa
     2761 tccttcagat atcggtttga ataatgcagg tgctgtagaa ggaatctctt atgcaacaga
     2821 ttggttcaaa aatgtttggc caaagggaat gcaagacata aaaagtgcag gagacttcgc
     2881 cagccagcaa ttcatgtcca ataaaactgc agcaatcatt gacggtccat ggcaagcgca
     2941 aacatacaaa gaaaacaata tcaattatgg tgtagccaaa attcctactc taaataatgg
     3001 acagccttat caaccgtttg gcggaggaaa aggatgggta gtcagcaatt atgctaaaaa
     3061 caaagacctc tctcaaaaat ggcttgatta tgtaacgaat caggaaaatc aagaaaaatt
     3121 ctttgaaatg gtaaatgaaa tccctgcaaa ccaacaagct agggaaacag caaaggctaa
     3181 aaacgatgaa ttaacaacag ctgtgattga acaatatgaa acagctcaag cgatgccaaa
     3241 catcccagaa atgggcgaag tttggacagg tgcagaaaac ttgatgttcg atgcagcatc
     3301 aggttcaaaa acaccaaaag aatccgcaga tgaagcagta aaaacaatca gtgaagccat
     3361 agaacaaaaa tactgattaa aatgataacc gtccattttt ataaaaaatg gaacaggtgc
     3421 ttcagataaa agtggcaaga gcgagatgga aaattatctt gctcttgttg ccattatctt
     3481 aaaagcagtt tgcggtttag caagtttaga gggggcattc agatgcagca agataaaaca
     3541 agcaatatca acgaaaaaaa aatcacgctt tgttcactta ttccagggat ggggcagttt
     3601 gtaaatggcc aagcttttaa aggtatcgtt tttttcgcca tattcgtcct attcattggt
     3661 cagatgcttc tgggcggatt tcatgcattg gtaggattgg ttactttggg aagtatacca
     3721 atggaagatc attcattatt tattttgatt gaaggcactt tgcaattcat cattacaatc
     3781 attttcttga ttttttacgg tttgaatctt ttagacgctt atcgagtagc gaagatgcgt
     3841 caagagggca aaaaaatcaa tcggacgatg aaggaagtca tacataatat aggtgatgaa
     3901 ggttttcctt atctattgac acttcccgct tatcttctca tgatttttac gatcattttc
     3961 ccagtcctag ttacattgtt cactgcattt actaattatg acttcaatca tattccacca
     4021 gccagtttaa ttgattgggt aggggctaaa aactttttta gtatcttctt tttaagctca
     4081 tatcgtaata ctttttttgc agtcttttcg tggaccgtga tttggacttt atgtgcaaca
     4141 actttgcaaa tcacccttgg tattctcaca gcggttgttg ctcaccagaa atttattaaa
     4201 ttcaaacgta ttttcggtat cattttctta ttaccatggg cggtcccagc ttttattact
     4261 atcatgagtt tttcgaatat attcaacgat agcgtaggtg ccatcaatac tcaagtgatt
     4321 cctttcataa accagcttcc ttttgtagat atcagtgcaa tctcttggaa aacggacccg
     4381 aactggacga aagtagcaat tatcttagtg cagggctggt taggatttcc ctatatctat
     4441 gtgatggtta caggaatctt acaatcgatt tcagaagact tatatgaagc agcaaaaatc
     4501 gacggagcaa atgcatggca gcgcttttca aacatcacgc ttcctgctgt cttcctagtg
     4561 gcagcgccaa catttattac acagtatacg gggaatttca ataatttctc catgatctat
     4621 ctgtttaaca atggcggtcc cggaagcgta ggcggaggag ctggttctac tgatattctt
     4681 atttcctgga tctataaact tacgactgga acttctccgc agtattctat ggcttcagca
     4741 atcacgttga ttatctccgt catcgttatc tgtgtgtcat tgctggtatt taaaaagaca
     4801 aatgcgttca atatggggga ttaacaatga aaaaatttca ttcgagtgct cgattccaaa
     4861 aacgactttc tcactttttt acttactttt ttcttttaat actgtcagtc attatcatct
     4921 atccgctttt gatcacagca agtactgcct tcaagtcagg aaatattgca gcttttagtt
     4981 tagatctttc aagtgactgg actttaggaa actttactcg gctattcagt gaaacacttt
     5041 acgggacttg gtataaaaat acattgatca tttctgtcct aacaatgatc gtacaagtga
     5101 ctgtagtaac tttggcgggc tatacttata gtcgctatcg ttttatcggc cgcaaatcaa
     5161 gtttgatgtt ctttttagtg attcagatgg taccgactat ggcagcattg actgcctttt
     5221 atgtcatggc tttcttatta gaagcgctcg ataagtactg gttcttgaca atgatctata
     5281 taggcggtgg aattccaatg aatacttggc tgatgaaagg atactttgac actgtaccaa
     5341 tagatttaga tgaatctgct aaacttgatg gggcagggca tttcaggatt tttttccaga
     5401 ttgtattacc gcttgtcaaa ccgatgctcg ccgttcaagc attatgggct tttatgggac
     5461 cttttgggga tttcatgtta gctaagttcc tgcttcgaac acctgaaaat cagactgttg
     5521 ctgtcggctt acaaacattt atttctgatg tgacaaacca aagagtctct ctttttgctg
     5581 ctggggcaat tttgattgca ctaccgattt gtctattatt cttcttttta caaaagaact
     5641 ttgttaccgg cctgacatct ggcggaacaa aaggatagga gcagagtatc atgcaaacat
     5701 ttccgttaaa ctatttttcc gcattacgta cgccaactca agtgtttgcg ggacgcaaac
     5761 tattatcatg gcctaaattt ttcttgatat ttgtattttt agtttccttg atggtcatgc
     5821 ctgtgactct tttttatgcc aatcagatcc aagcgattcc attagaacaa tttctatccg
     5881 ttcattcgct gatcgatgaa caaggaaccc aaaaattttc tgaacttgaa ttatctgaaa
     5941 ctgggttaca agcatcgcaa caaacgatag ctgtgacccc tgaaattttg gtaggtgtgt
     6001 ctttatctga gaaacaacaa tccgatcatg gaacttttat tgattttgag aaggaacaat
     6061 gggtgatcca acaaaaggat aaatctggta tacgccgcta tacgatgaat tatagcccat
     6121 ctttccaacc agattctgta cgtacgcccg aagatttcca acgattttta gaaagagaat
     6181 tttatgccag taatcgtcca acaatcattt tatcttatag tttaagtctt ggtcttgtcc
     6241 tatttgtcat gacgagtctc attttattcg gggcttcctt tttcttgtgg atgacaagaa
     6301 aaagccaact ttcatccatt catacattca aagagagtgc aaatttgatg ctgaatgtta
     6361 tgggaatagg ttccatggca gcagcagttg tagggtttat ccactttgac tttatcttga
     6421 tgctaagtgt ccaaacaacg ataactgtct tgctgctttt atgggttttt gtcaaaacaa
     6481 gattcaaaga taagcgtgtc gaatgacaag tttaagcgat ttatgcgaca atgaatgtga
     6541 aaaaaatgaa aaataaagta gtttttgaaa aatttctgaa aaaattaaac ttcttagttg
     6601 attcttttct tttttagtat taacgtaata gggtagtttt tttaaggagg aaaatcatgt
     6661 tagagaaaac tttatacaat caattatttt cacgttcttt ctcgctccct gtcgaagtaa
     6721 attattggga cggtacaacg aaacaatacg gagatacgga cactcctccc caaatcaaaa
     6781 tcaatatcca tgaagaaatc ccaatgaaag agattacaag taacgcatct ttggcactag
     6841 gagaagccta tatggatcat cgaatcgaga tcgagggaag tatccaagca ttgatcaacg
     6901 atgcttatca gcaagcaaac agctttttac gaggaaaaga ttatctaaaa tggctgccaa
     6961 agaaagaaaa acatacgaaa caaaaaaaca aagaagatat ccatgcgcat tatgacttag
     7021 gtaacgactt ttataaactt tggctagatc caactttgac ttattcttgt gcttatttca
     7081 atacacctga agatacgttg gaacaagcac aaatcaataa agtccatcat atcttagaca
     7141 aactctttat caaagaaggc gaaacgttgc ttgatatcgg ttgtggctgg ggaacattga
     7201 tgttcactgc taccaaagag tataatgtca aggcaactgg gatcacacta agtgaagaac
     7261 aatacgattt gattaccaaa agaataaaag aagaacattt agaagataaa tgccgtgtat
     7321 tattaatgga ttaccgcgaa ctaaaaggcg aaacgtttga tcatatcaca agtgtaggta
     7381 tgttcgaaca tgtaggttct gaaaatcttg aaggttattt caaagtggta aaagatttat
     7441 tgaaaccaaa aggaacagct ttgatccatg gaatcagccg acaacaaggc ggtgcgacta
     7501 atgcttggat caataaatat attttccctg gcggttatgt ccctggtgtt actgaattag
     7561 ttgataatat tacaaaaaac gatttgcaac tgattgattt ggaaagctta cgtcgtgact
     7621 accaattgac attagaacat tggacaaaga attttcatca agtccaagat caagtcacga
     7681 aagaaatggg tgattccttc tatcgaatgt gggatctata tttacaagct tgtgcagctt
     7741 catttgaatc aagtaatatc gatgtgattc aatatttgct tgttcaacct ggaaacaatg
     7801 acatcccaat gcatcgtgca taattgaggc aaaagtactg tttaaagact atttaaaaag
     7861 aagctaagtt ttatgatgct ccagaagtga acttttttgt ctaacttcta ggattgtctc
     7921 taaagcttag tttctttttc tttatatttt cataataaaa gtttggttct ttaattttta
     7981 aacatgacag aaaatttttt cttcgttata cttgtaatag aatactattt tataacaaag
     8041 gggatcgaat caatgaaaaa aactgttatt tatactagtt tatttgcagc tttgttttta
     8101 gctgcttgtc aatcaaatac caatcaaggc acatctggga atacgtcttc cagtagcgaa
     8161 cagcgctctt cacaggtatc ggactcctca acaaaagcaa gcacatcttc gtctagccag
     8221 acaaaaagtt ctagtgaagc aagtactcag tcagatatca aagtgtctgc agctcgtgcg
     8281 atcgaattgt tccaagataa atacccagat actgcaatta ctagtttgga attagatagt
     8341 gattggggat cttacttcta caagatagaa ggtgtcgatg atcaaaaaga atacgaagca
     8401 agaattgatg ctcaaaatga aaaaatggag acagagaatc cagaaactct agatcgagat
     8461 gaacaaaatg gtcaaaagaa aaaagaagat ggtctggatg ttgataaatt gatcacgatt
     8521 gaggaagcag caaaaatcgc cgtcgacaaa gtaggaagcg gtacggcaac agattgggat
     8581 ttggataaag atttaggtac tacttattgg gaagtcaaag taaaagacgg caatcaaagt
     8641 acatcagtca agatcaatag ccaaacaggc gaagtactgt caactgaaca agatgattaa
     8701 gaatttcttc ggaaaacaag agttgatttt tcttttagtc atgatacact agacaaaata
     8761 acttgtttga aggagcgatt tacttgagtt tagaaacgac ctttgccaat catatctttg
     8821 ccaatccttt gatgaatgca tctggtgtcc actgcatgac tatccaggaa ttagatgaac
     8881 tggctcattc agaagcagga gcatttatca ctaagagctg tacgatcaat gaacgaaaag
     8941 gaaatcccga accacgctat tttgatgttc ctttagggag tatcaattct atgggacttc
     9001 caaacttagg tttttcttat tatctagagt atgctcttgc gtatgaaaaa gtgcaggaaa
     9061 accaaaatca accgttattt ttttccattg ccgggatgag tgtccaggaa aacttggaaa
     9121 tgcttgagaa aatagaaaaa agtgggttta atgggatcac tgaattaaat ttatcttgtc
     9181 ccaatgtgcc tggaaaacca caactagctt acgattttga ggccacttat gaaacactga
     9241 aagaagtatt cagcatcttt tctaagccgc taggaatcaa attgcctcct tattttgatt
     9301 ttgctcattt tgatcagatg gctgatatct tgaatcagtt tccattgacc tatgtcaatg
     9361 ctatcaacag tgtgggcaat ggtttgtata tcgatacaga acaagaagca gtagtcatca
     9421 aaccaaaaga agggttcggc ggcattggcg gagagtatat caagccgact gctttggcga
     9481 atgttcgcgc tttttataca cgtttgaaac cagaaatcca aatcattggt acaggtggga
     9541 ttcggacagg acaagatgca tttgaacatc tgctatgtgg cgcaagcatg ttacagattg
     9601 gtaccgagct tcataaagaa gggccggaaa ttttttctag aatcataaaa gaattaacgc
     9661 agattatgtc agaaaaagga tatacgtcaa ttgacgaatt taaaggcaaa ttacgcacca
     9721 tttcatagca aagagacgaa ggtcatgatt gatcttcgtc tttttttata aaaaacactc
     9781 gtctgttcat tcctgtgcaa gcgtactcat tgtatgttag acgaacttgt gggaaaataa
     9841 cagaggcaaa aaggaggata atatgaatga tgcaacagaa ttagcaaaag agcttaaaga
     9901 aaaagaattt agttttcaag aactagttac ggatatacag aaaaaaataa agaagcaaaa
     9961 tcatgaattg aatgcgtttg tcgcgcttga accattcgag agtaattccc acgagctatt
    10021 gattccccag tcagaaaaag ggccattggc tggtatccca tttcctttaa aaatgctcgg
    10081 tcaagaaaaa aagggatggc tagctacttc aggatctcgt ttatttgaaa cacaccgagc
    10141 ttctcataca tctaattatg tgcggcaagc agaagcgata ggtctagttc cttttggaca
    10201 aaccaatgcg ccagagtttg gtttcaaaaa tattacagac ccagtcatct atggcccagc
    10261 aagaaaccct tggaaccttg aatgtacacc gggcggatca agtggaggag ctgctgctgc
    10321 agtttcatca ggaatcgttc ctttagctgg tgctagtgat ggcggtggtt ctattagaat
    10381 acccgcttcg ttttgcggat tgattgggct gaaaccttcg cgagggacga tgccagttgg
    10441 tccatatgct tggagaggat ggcaaggagc tgctattgat ttcggcttga cagtttctat
    10501 gagagacacg gaggctttat tttatgggat gagaagtatc cattccggtg cgccatatca
    10561 agtatcgcca gttgagtggc aaacacatcc aagaaaaaaa cggttgaaga tagctttttg
    10621 tgttgattca ccgatccact cacagattag tgaagaggca gtacttgctg taaaaaaagc
    10681 tgtagatttc cttggaaaag aagggcatga tttgacagag attccttacc cactagatgg
    10741 tcgtaaactg atcacgtctt attatcagat gaatggcgca gaaacagcag caatgatccg
    10801 ctcgattgaa acaggcatcc agcgagctgt cagaaaagaa gaacttgaac ccatttcttg
    10861 gactttgttc caatatggtc agaagctctc tgccgctact tacatcgatt ctctgcatgt
    10921 atgggatgaa gcagcaattg tcatggaaaa cctacatcaa gaatacgatc tattcctttc
    10981 accaacgact gcatttaccg cccctaaaat cagcgaagat ttgcaaagtg atgcgataag
    11041 agcacagatg gaacacatca gtgattgttc tgaagtagaa ggtttagacg ttgtttcttc
    11101 catgtttgag gagagcttga agatcacacc ttatacacag ttggctaatc tgactggaca
    11161 accagcaatc agcttgccta ctcatttgac aaaagaaaac atgcctctag gaatccaatt
    11221 tatggctgcc agaggaagag aagatttgct atttcaaatc ggctatctat ttgaagcaaa
    11281 tcatcagttc catttaccaa atccatggaa atagcagaaa caaggagaag aagaatgaag
    11341 aaaaaaagat tgtttgctct aataactagt gcctttttta ttttaagcgc ttgcggtaca
    11401 gctgccgcta atcaggaaga taacagctgg agtaatgtag aaaaaaagaa agaattagta
    11461 gtagcgactt ctggcacact gtttcccgca tcttattata atgatcaaaa tgaattagtt
    11521 ggttatgatg tagatttggc aaaagaggtg gctaaacggt taaatgtcaa aatctccttt
    11581 aaagaataca acgtggatgg acaaatcact tctgtttcaa aaagagaatc agattttgct
    11641 gcgaacgatt ttggtatcac aaaagaaaga gaaaaaaaat tctccctttc agagactttc
    11701 aaatattcat ttgacagtat gatcgtacga aaaaaagatg attcaggaat ccactcactt
    11761 gaagatctta aagggaaaaa agcagctggc gaacccaata ctagctatat gcgtttagcg
    11821 gaaaaacacg gagctaaatt agtcacatat gacaatgcca cgaatgatca gtatttaagc
    11881 gatgtagcaa atggtcgaac agatgttatt ctaaatgatt attatctgca gaaaatggcc
    11941 gtatctgctt taccagatat ccctgtaaaa atactggaga acgtttattt caatgaaaac
    12001 gaaagtggtt ttttattgaa agaaggaaat gatgctctga aaaagaaaat cgatggcgta
    12061 ttaaaagaga tgaaagaaga tgggacgaca aagaaaatat ctgaaaaata ttttcaaact
    12121 gatgtttcag tcaaaccgaa agaaacgatg gctgagtcgg tgagtgagtg atgtactttc
    12181 ctctaattga tttttccctc atgtggactt cacttccttt cgtactgcaa ggcttggtat
    12241 acacattagg gattggcttc gtgtcgtttc ttttaggaaa tctggtaggg ttgcttttga
    12301 cagttttagg tcttttggat tggctgcctc tgaatgtatt tattcgtttt tatttatcgt
    12361 tctttagagg cattcccgca ctagtcctgc tgtttctgtt atattttggg ttaccttatc
    12421 aattaagtgc cttaacggct tcagtcatct gctttacgat taccagcagt gcatttattg
    12481 gtgaaatcta tcgaggatca ctagcaggcg tttcttcagg tcagtgggac gctggtttag
    12541 cttctggttt ttctttcttt ggggtattga aatatatcgt tttgccgcaa gcttttcgaa
    12601 tctcagtacc ggcactcagt aacgtagcta tggatctagt gaaaggtact tcgttagcag
    12661 caacaatcac agtgccagaa atatttcaaa aagcaaagat cataggcggt cggacattcg
    12721 attatttttc tctctatatt ttagttgcac tgatttattg ggtcatttgt atcgggatcg
    12781 aaaaagtgca acagtattta gaaaaacgtt ctatgcatca gtcaatctga aaaatagaaa
    12841 tggtgctagc gtggctggca ccatttctat tttttgtttc ttgtatattc tgcctagtat
    12901 tttaaaacca tttcatgtaa aatgtaaatc gaaaatatgt tcggaggtag aaaacatgaa
    12961 gaccccgcta accatcgaaa tagaatcctc gttataccaa tattgcatcg agcaaggcgg
    13021 cttggttgta gaagaagtca cgatgcctga tgaccaagga attgtagata cgcttagctg
    13081 cttgacaaaa tttgatggaa gccgagaatg gcgatgctat gagctgaaag tgacaaaagc
    13141 agattttcgc tctacagcca aactgtcatt tgttggacat tataattatt ttgttttacc
    13201 aagtaaacta tatgaagaag taaaaactga aatccctaca ggaataggtg ttttagtgta
    13261 tcgtccgtac acggttgaag aggagatgcc tgcctctggt acatttctga ttgcgaaaaa
    13321 agcagcaaag aaagagcttg ctgtaccgga agaagctttg acaaaccgct tcatggcttc
    13381 tttattcagg gaagtaagaa aagcaaagca aatggagcga ggcgtaaaat attttccaac
    13441 agatcaattg tacaaagaat tgaaaagacg ttcagaaaat caagatccat tttctaacga
    13501 acgatattat gaacggtttt tagaggaaat agctagtcag gcaatcaatg agttgcaaga
    13561 agaagttatc gcgcttcagc atgattatga atatttgagg caacaacagc agatccgtcg
    13621 gttgcctaca gagccattgg agtgaaaaat atgtactatc cttatttaag aggaaaacaa
    13681 tttgacctat tagcattaaa agaagcactg tcgcgaggat tgctctccaa taaaatccaa
    13741 ccagtgatcg aaccagtacg tgattccgct acgcttaaaa atgtgatcga attatttcaa
    13801 aaaaagcacc atcgaatcgc tgtgatccaa aatccccaag taggacaatt caaattattc
    13861 gatcagcatg tgcacagttg gacagtcaaa gaaaatagct ctgtcgtccc tgcacagatc
    13921 ttaacgccag aaaatctcag tgaagtactg gattcacctc cagcgtttct tgttttcgat
    13981 ggacagcact acccaaaaga tacagaggtg tggaaacagt tggcaggagt tgattcgaaa
    14041 tttttgatac cagatactag tcgctttcgc atctggctgc ccgaaaacaa aattgtgatc
    14101 cgtgactctt ttcaaacgag aaagcatgtt gaaagttatg cagataaaac agatgacttt
    14161 tttagtgatg actatctttt tttccacgca gacggatata ttggattttc tgatttcacg
    14221 atcgaaggta gccgttattt cgataaaggg ttccctagcc gagcgcttgc tatccatcta
    14281 acgtatattg atgcttacgg caatatacgc gtgaaacatt ttgtctctga cagtaacgac
    14341 tctgctaaag accaagcatt gaagtttctt gaagcaggag ataaaatgaa aaaatggatc
    14401 atgcgtcacc atcatcaact cttgattact tcaggaatga ttgaattatt ggctctttac
    14461 cagcaacaaa aattccctgg actgggagtg ttgaaaaagt ggtcacttat gcatcatttg
    14521 gaacttatta gtcacctgct agatcatcca aaagattggc ttaaatatta ttcaaaagtt
    14581 caggaactat atgaagtgat ccaatgatta gataatgtaa atgaaaaagg aatagaaaag
    14641 aggaaactgg atgtatattc ggcaaacaag gaaagaagaa gtagaagaag tggtagaaat
    14701 cattaatgcc gcagcggatt tcattgcttc gcaaggctct cctcaatggc aaaacgggca
    14761 gaaaccaaca aaagaaacga ttttgaacga tattgaaaga aaagaaagct atgtattaat
    14821 agtggaagat caaattgctg gaacagctgc attagtctca agcgtggatc ccgtttacac
    14881 aaaaatagat ggtagttgga aagaatcaac tggtccttat ttatcgattc atcgtgtcgc
    14941 tttagcagaa agtactcgag gaaaaaaact agcaaaaccg ttcttagaac atttgttgac
    15001 gattgcagcc agcaaaaata tccaagatat ccgtatcgat acttatccag ctaatgaacc
    15061 gatggaaaag accatctatt ctgctggatt cacttatcaa gggatgattg aatttccttt
    15121 tgaacacggc gaaagaaaag catatcaaat aatcatcaat tgactataaa atcatctgag
    15181 caaagataaa gacattaaca ctactgaaaa aaacaatcat atgatagcca agaggttgta
    15241 acagaagtgg ccagcttcaa aaaacaagaa agaatttccg aaaatgcttc tcaaattttt
    15301 gtggaatttt agcgtatttt acgaaatagc tgctttctga aactcccttc atcgagaggt
    15361 cgtgacatag cttttgtcac gacctctcgg cttatcctac gagtattatt cgatacattt
    15421 atatcaataa tccttttttt attttatctg cagcttcttg gaacgctcca tcgtttgaca
    15481 cgactaaaag tgaggggata gctttcaaac gtttcaactg tgctaaatct tctacataaa
    15541 ccgccagtct ggctgaaatc tgttcagatg aatctccacg ttttttcagg cgcgattgca
    15601 atgtttcttt agagatcgta ataaagatag gacggactac cgtcccgaaa tactcatata
    15661 aatgccaaaa accatttgct gtcaaaacgg cgtaacaatt tcctactgtc agtttctttt
    15721 taatttctgc tttactggaa ccatatttat gttgagcata ttggtcccac tctgcaaact
    15781 catttccctt gatcatctga ttgaatgtat tgtcagaaac aaaataataa tcctcttgat
    15841 ctttttcccc aggtcttggt gatcgattcg tgtaagtaat gatcttttgt tccggctgaa
    15901 aaatagcttc tgcgagtgaa gtttttccag aaccagaagg accgataaat acatagcaat
    15961 aatt
//
LOCUS       DAKBVG010000048        15940 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000048 DAKBVG010000000
VERSION     DAKBVG010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 15940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.048"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            35..730
                     /locus_tag="OUD09_001073"
     CDS             35..730
                     /locus_tag="OUD09_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCT9692355.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            complement(1172..1969)
                     /locus_tag="OUD09_001074"
     CDS             complement(1172..1969)
                     /locus_tag="OUD09_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HCT9692356.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(1966..3150)
                     /locus_tag="OUD09_001075"
     CDS             complement(1966..3150)
                     /locus_tag="OUD09_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HCT9692357.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(3175..4422)
                     /locus_tag="OUD09_001076"
     CDS             complement(3175..4422)
                     /locus_tag="OUD09_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HCT9692358.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            4637..6382
                     /gene="dnaX"
                     /locus_tag="OUD09_001077"
     CDS             4637..6382
                     /gene="dnaX"
                     /locus_tag="OUD09_001077"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCT9692359.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            6402..6716
                     /locus_tag="OUD09_001078"
     CDS             6402..6716
                     /locus_tag="OUD09_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCT9692360.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            6741..7337
                     /gene="recR"
                     /locus_tag="OUD09_001079"
     CDS             6741..7337
                     /gene="recR"
                     /locus_tag="OUD09_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCT9692361.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            7401..8045
                     /locus_tag="OUD09_001080"
     CDS             7401..8045
                     /locus_tag="OUD09_001080"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCT9692362.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            8060..8389
                     /locus_tag="OUD09_001081"
     CDS             8060..8389
                     /locus_tag="OUD09_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HCT9692363.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            8403..9341
                     /gene="holB"
                     /locus_tag="OUD09_001082"
     CDS             8403..9341
                     /gene="holB"
                     /locus_tag="OUD09_001082"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCT9692364.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            9377..10201
                     /locus_tag="OUD09_001083"
     CDS             9377..10201
                     /locus_tag="OUD09_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HCT9692365.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            10194..10541
                     /locus_tag="OUD09_001084"
     CDS             10194..10541
                     /locus_tag="OUD09_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HCT9692366.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            10610..11482
                     /gene="rsmI"
                     /locus_tag="OUD09_001085"
     CDS             10610..11482
                     /gene="rsmI"
                     /locus_tag="OUD09_001085"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCT9692367.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            11591..12712
                     /gene="dinB"
                     /locus_tag="OUD09_001086"
     CDS             11591..12712
                     /gene="dinB"
                     /locus_tag="OUD09_001086"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCT9692368.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(12766..13368)
                     /gene="nrdG"
                     /locus_tag="OUD09_001087"
     CDS             complement(12766..13368)
                     /gene="nrdG"
                     /locus_tag="OUD09_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HCT9692369.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(13683..15872)
                     /gene="nrdD"
                     /locus_tag="OUD09_001088"
     CDS             complement(13683..15872)
                     /gene="nrdD"
                     /locus_tag="OUD09_001088"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCT9692370.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
BASE COUNT         4960 a         2979 c         3338 g         4663 t
ORIGIN      
        1 tacctttgga ctttaaaaag ttttaaagta ctgtatgatc tttttagatg aaagtttgga
       61 tgggggagca tcattggata gtagagtctt aaaggaatat ttggataaaa atgattttcc
      121 gattatacgc aaaggatatc ataaatattt gacattcgaa gggatggaag attcccatac
      181 gtatatcttg aaagagggaa cggtaaaagc aagtgttatt tcaaatgatg ggagagaatt
      241 taaccttcgc tatatcaaag atcttgaaat cgtctctctt ttaaaagacg aatattcaca
      301 attcatcgat tcgccgtata atattcgagt tgaatcagaa tatgcagagt tttatcgaat
      361 caatcgtgtg cgcttttggc aaaatatcaa ccaagaccca aagctacaac actatgtaaa
      421 agagtattac cgctatcgtc tgctttattc gatgaaaaaa atgcagcaaa tgctattaaa
      481 cggaaagctg ggagctgttt gtacgcaact ttacgaatta tatgatttat tcggtgtgcg
      541 gacagaagaa gggatgctga tcgatttttc gatcacgaat gaagaaatcg ccaaattttg
      601 tggcatctca actgctagca gtgtaaacag aattttaaaa caactgaaaa aagatggtat
      661 tatcaccgtt aaagaccatc atatcgttat tttgaatatg gacttgttag aagacaatat
      721 tatcttttga tgaacaaaga ggttgtgaaa tcagcgtttt gacctgagta ttagagaaac
      781 aaacggaaaa atagttcctc atattttgcc gttttttgag ctaataccga gggtcagctg
      841 attgaacacc gtttaccaag aggttgtgag cctgccgttt aactctgagt cacaaggaac
      901 aatttcaaaa aacagctttt catgtttgat gaatattgtg acttgtgacc gaggagttgg
      961 taggagaaca ccgtttacca agaggttgtg aaaaagctgt tctgcatcaa gtaataagaa
     1021 gagccaagta aaaacagtct cttatgtttt taacttggct ggacttatca ccgtagatgt
     1081 aagcttttga acgccgttta tttgcaacaa aaggggctgt gacacgattt ttgtcacagc
     1141 cctttttttg cgaacattca tttagcaatt tttagaaact tctgccgcca ccgccaaaag
     1201 tcccgccacc gctagaatgg gtagtacttc cgccaccgcc gcttccagga ggtgggcttt
     1261 tggggattct tcttgtcgtg acaaaagaat ttgtcagacg atcggttttt tcagttaatt
     1321 tgatactgga tttttctcgg aatggatatt tgtagatgcc tgatttcaat tgataacgcg
     1381 aaacagtcac aaaataaaag acaagactaa ggataagagc caaaacaaca gcaatgatga
     1441 tttctactgt cgtcaaaacc ttatagcgtg taattttgcc agtttcttcg tctacacgat
     1501 aatgtcctcc tggaactccg tcttccacat atgttgctga tttagacagg taagcttttg
     1561 cagcattgta ataggcagca tttgtcatct gatcataaac atcatctaag atcgaatcga
     1621 ttctgctatc agtcaaataa tcgatcatat taccagaagt cgaaatgtag atttcacgct
     1681 gtcccatatc taatagcaat actgagccat tttgatcatt tccgactgcg tctcttaagt
     1741 aattatcagc aaagtctctt ggttcttctg aattagatgt agtcgttacg atcaagactc
     1801 gtcctttgat tttttcattc agcttttctg cctgttgttc caaagattgg atttgatctg
     1861 ttgtgaataa cccagcctca tcatttaccg tagctgtatc tgcatgagta gccaccggcc
     1921 agccaacact tactagcaaa agaaagaata aaaaaaattt tttcatcata tcaagtaacc
     1981 tccgatcaga aagaggatcg ctagcccagc gaaaatccca agagcggtca gtcccaattt
     2041 tttctggctg atcggcagga caccgctgac ctttcctgtt tgtccattca ttgcgtagta
     2101 gtagactttt ttacttgagt cattgcttcg ataagtaaca agccataccg gcagtaatac
     2161 gtaattattt acttcgtttg tgattgaagc agaagttcgc acaccggtca atgttgtata
     2221 tccatttgcg gtatctttta acagcttctc tgaatagtct tttaattcag attgaatctg
     2281 tgatttgatt gcctcatatt cgatatctct tttttctgct tggaacccgg ctaaatactg
     2341 gcttttaaaa tcaattgctt tatctaacgg gaaaggttgc acgccttcga ccatcttttg
     2401 ttgcgtattt tttgataagg cattttttac tagttcttta aaagaaagct tgcctgctct
     2461 tgaaacagca aattgcttcg tttctgtgta ttcgatgtca cctatccgcc atatgcggat
     2521 aactgtacca ttcgcctgca tggcaccatc tacttcagca tctgtagccc aataagggaa
     2581 atacacacct gtcaacttat cgatttgatc cttattgaaa aaagctttgg gaacaaacca
     2641 ttttttcttg gtccaagcga gaaatctgtt gattgcttct tctttctcga cagcaaaagg
     2701 aagtacttta tttggcaaga attctccgct taaacgtccc gatagtacga cgggattatg
     2761 gcagtagtaa caataagtcg cagcagttgt cgcatcagtc acgatctctg caccacagtt
     2821 gggacaaaga aaaagatcta tcgctgcggt attatctgca gattcgtccg ctgtatctga
     2881 aaaaccgcct gcctcgtcaa ccgctttgcg ctcggcttca ttcatatcgg caagctgttc
     2941 ttgggctgta aaagtaaaat cttcggtagg tgaaggttct tctttgcttt cttcgccagc
     3001 catacgcgct tctttttgct tttgctcata ttggctgact tcttcttctg tatagatatt
     3061 caggcagtat tcgcaatgaa atttttggtc tttgggatca aaagtcaacg gaccgccgca
     3121 attaggacat ttatgtgtaa gaacatccat cagctgcaca cctttctaaa taaattaatt
     3181 aacggggatt cctttaaacg gtttaccaca gtttggacag aatttaggga tgccgttgga
     3241 aagatccacg atctcattgc attcactgca tcgcatttgt agcttaggct gaacgttttc
     3301 tacaggtttg gcagctccgc agttggaaca gaattttccg gaattttctg tcccgcaaac
     3361 tggacatgtc caagtatccg catttccttg tgcagtttgt tgattttgtt tttctgcttg
     3421 ttgtttgatt tgttcttggt tattttgagc tgcttgagaa agataagaac catttgcatt
     3481 cattcccata cctacaccca taaagccggt cattgctcct gcttcatttt ttccggctgc
     3541 ttccatacca cgtgcaatgg aaccttgcac gtatccttca cgtacgctag gatcacctag
     3601 catcgcccct tcgttacgca tattgatcag tttcacagaa tcatcggtgt aagaaatgct
     3661 ggctacagcc acagaaacaa tctccatccc acgtaattca cgccaagaat catccaatgc
     3721 agtatccatg tatttgctta attccaaact cttagatggt acataagaaa tccgttgacc
     3781 atctgcagac atctgattga ttgctgattg tagcgctgtt aggaattcag ctaaatactg
     3841 ctcattgata tcattgattt ccacttgtgt tttgttttta gggattgcgt ttgtataaaa
     3901 taaaatcggg tcaacgacat gaatagaata agtgccatgt gcccgtaaga ataattctgc
     3961 gttatagaaa ttgtcaaagt agttcaacgg cgcgcttgta ccgaatttga ttccctttat
     4021 ttcttggaga ttgatataaa aaacttgttg tttctgcggt gtgacgccgc caaatttgaa
     4081 tcggtcaaaa gtttcagcaa ttgcttcttt cagtgttcca ttgaacatgg aaggagctgc
     4141 gtcattttta atggtgtaat aaccttcctc agcggtataa tcgatgattt ttccaccatc
     4201 gaccaaaagc atcatcatgt ttggatagac atggacgact gttccgtctg ttaaaacatc
     4261 ttctgttcct ttacgattgg agccacgttt gtcatctttg cggactttga caccttttgt
     4321 catgaccgtt gtatcactca tattgtctgg ttcgatgact tctagccatt gatctgccag
     4381 accgccgcca accatacttg ttgctgcttt gataagaccc ataaaaaatt cctcctctga
     4441 ttcagctagt gccaaacaat tattgtttat ttattatacc ataggaagat gtgaaacttc
     4501 tcggactaga gacggaattg aaatggaaaa ttgcgtattg tttaaatacc tgaaaaagcc
     4561 gagaaggata gtagtttttt tacctaatat cacgtataat aagagatagt gaatttttta
     4621 aaaaaggggg atccttatgg catatcaagc actttatcga gtctggcgtt cgcagcgttt
     4681 cgaagacatc gttggacaaa aagctgtgac acagacacta aaaaatgcta ttgtttccca
     4741 caaaacctca catgcctact tgtttacagg acccagagga accggaaaga cgagtgcagc
     4801 aaagattttc gcaaaagcaa tcaactgtcc gaacagtaaa gacggtgagc catgcaacga
     4861 atgcgaaatg tgtcgatcaa ttactgctgg tacgcaagaa gatgtgatcg aaatcgatgc
     4921 tgccagcaat aacggagtcg aagaaatccg atttatccgt gatcgggcaa attatgcgcc
     4981 tacaaaggca gcgtacaaag tgtacattat tgatgaggta catatgcttt caacaggagc
     5041 tttcaatgcc ttgttgaaaa cattggaaga gccaaaagaa agtgtcatat tcatcttagc
     5101 aacgaccgaa cctcacaaaa taccagctac gatcatttca cggactcagc gatttgattt
     5161 taaacggatc aatacggcag atattgtcga acatcttgaa tttatcttga aagaatcgac
     5221 tatcagctat gaaccagctg cattgaatgt gattgcccgt tctgcagaag gcgggatgcg
     5281 ggatgcattg agtattcttg atcaggcaat ctcgtttagt gaaggaacaa ttactttaga
     5341 tgatgccatg caagtgacag gtagcttaac tgacgaaatg atggacagtt acttgagtgc
     5401 ttgcgtgaaa aaagaagtac cagaagcatt ggaaacattg aaccaaattc tcgcagccgg
     5461 caaagaatca cgtcgctttc tagaggatat gttacaatac tgccgtgact tgctgatgta
     5521 tcagcaagca cctaacctag ttgaggaaca atcgagtacg atcagaagta catttacaga
     5581 attggcacaa actgtttcag cggatcagct gttttcaatg ataaagattt tgagcgatac
     5641 tcaaaatgaa attcgtttta ccaatagtgc cgctatttat ttggaagtcg ctacagtcaa
     5701 acttgcacag ctaccgtctg cttctgttcc ttcagcagat gtagcatttg cagatgatca
     5761 gatagtaaaa cagctacaac aagagattaa ggaactaaga aacgaaatca aggaactacg
     5821 cagtaataca ggaattgaga atacaaaagt acagccggta cagcaaacaa atcataaaaa
     5881 acagacaagt acctatcgtg taccgacgga acgagtatat aaagttctgc gggaagcgac
     5941 aagacagcat ctgaaccaag taaaagaagt atgggaagac ttgctgatga gtctatctgt
     6001 cactcagcga gcaatgctta aagcaagcga accagttgca gctggaccta caggactagt
     6061 gattgcgttt gattatgaga ttgtttgtca aagagcagca aatgacgaag aattgcagct
     6121 ttcggtacac aacaacttaa gccgaatgat caaagattat gcgcctgatc ctgtttatat
     6181 tacaagagaa agctggccag aacttcgaag aaattatttg acgcaggcac aagaagaggg
     6241 aacagaaaac tcttttgctt ctgaagactc agaaggagaa atcgagttgc ttcctcaaga
     6301 acaagaagta ccagaagaaa cacttgtagt aaaagcagag gaactgtttg gctccgaagc
     6361 tgttacagtg gtagatgatt aataaaaagg agtagataat tatgatgcgt ggaatgggca
     6421 atatgcaagg catgatgaaa caagtacaaa aaatgcaaaa agaaatggca gaagcacaag
     6481 agctgctaaa taaaacagag tttacaggta aagccaccaa tgaattggtc aaagcggttt
     6541 ttacaggcga tcgtcgcatg aaagatatcc aaatcaatga agaagtcgtt gatcctgatg
     6601 atatcgagat gcttcaggat cttgtcgtaa tggcagtcaa tgatgcgatc gcacaaattg
     6661 atgtggaaac tgagaaaaaa ttagggaaat acgcaaaagg tttgccagga ttttagtttt
     6721 aaactgaagg agtaatgaat atgcaatatc cagaaccaat tgcgaaactg atcgacagct
     6781 acatgaaact tccaggaatc ggtcagaaaa cagcgacaag actcgccttt tatacgatcg
     6841 atatgaaaga tgaagtagta aatgaatttg ctaaatcttt gttaagtgta aaacgcgatt
     6901 tgcatttttg tagtatctgc ggaaacatca cagaagaaga tccctgcgaa atatgtagaa
     6961 atccttcacg tgatcgaagc gttatcctag tggtcgaaga gccgaaagat gtaatggcaa
     7021 tggaaaaaat gcgagaatat cacggacttt atcatgttct ccatggtgtg ttatcaccga
     7081 tggaagggac aggtccagaa gacatcaata tttcttctct gttgcagcgg ctgcatgatg
     7141 cagatgtaaa agaagtgatt atcgcaacaa atgcaacaac agagggagaa gcaacagcta
     7201 tgtatctctc acgtctgatc aaacccgcag gtatcaaagt gactcgtcta gcacatggtc
     7261 tgtctgtagg ctctgatatc gagtatgcag atgaagttac tttattgaaa gctgtcgaag
     7321 gaagacgtga gttataaatt tctaattaag tgttttttca gatacaatat aaaagcagca
     7381 caagagatgg gggcaacaat gtgaacggac tatttattac aatcgagggg cctgatggtg
     7441 caggaaaaac cagcatcttg aatgaacttt accctctttt gaaagaagta gcaaaaacag
     7501 aaatcattca aacaagagaa ccaggaggca ttccgattgc tgaagagatc cgagcagtga
     7561 tccttgatcc aaaaaatgat cggatggatg aacgtacgga agcactgctt tatgcagcag
     7621 caagaagaca acatctagtc gaaaaggttc tgcctgctct ggcaaaaggg aaaatcgttt
     7681 tatgcgatag atttgtcgac agttctcttg cttatcaagg tgcaggccga cgcatcggcg
     7741 ttaccgagat tgctcggttg aatgagtttg ctacagaagg tactacgccg gattttaccc
     7801 tttatcttga cgtagattca gacaccggat taagaagaat caagaaaaac agacaaaatc
     7861 aaatcgaccg tttagattcg gaaggtttgg aattccacca gcgtgtacgt catgcttact
     7921 taaagctggc agaagagaat ccggaaagaa tccacaaagt tgatgcaaga aaaagctttg
     7981 aagaagtgct gcaaacaagt tatcatacaa ttattgaaca gtatccgcaa ttttttgaaa
     8041 actaggaagg tgaacgttta tgaaaattat tttagcgatt atccaagaca aagacagcaa
     8101 ccgtttatca aatgaattga ttgatgcaaa tatccgtgcc acaaaactat catcgacagg
     8161 cggcttcctt aaggctggaa acagtacgtt tatcgtgggt attgaagatg accgtgtaga
     8221 agaagcattg gaaatcatca aaaaaacgtg tgaatcaaga aagcaatttg tatctacacc
     8281 tgttacactg gatatttcga tggatggggg cgtcccttat ccagtagaag tagaagtagg
     8341 cggtgccaca gtcttcgtct tacctgttga aggattccat caattttagg aggagctgag
     8401 ctatgaatca agaaataaac ttggctcaaa tgcaaccgat cgtgtatcag cagcttcaac
     8461 ggagttttga gcatgggcgt atcgctcatg cttatctttt tgaaggggag aaaggaacag
     8521 gaaaacatga agtaggtata tggcttgcac agcacctgtt ctgtacccaa atgaaagaca
     8581 atctgccttg tggaaaatgc aacaattgtc agcggatcga aaagaaagaa catccagatg
     8641 ttttggttat tgaaccggaa ggacaaacca tcaaagtcga tcagatcaga cgtttgcaga
     8701 cggaattcag tcgcagcggt tacgaatcaa gaaaaaaagt ttttttgatc aaagaagctg
     8761 aaaaaatgaa tagcagtgcg gcaaacagtt tgttgaaatt cttggaagag ccccctggag
     8821 attttttagc ggtgttagaa acagatgcag taggaagaat tctccctact attcaatctc
     8881 gttgtcaagt tctgcatttt caagaacttt cgaaagaagc actgatccat cgattgcaag
     8941 aagagcagat tccattggaa aaagcaaaac ttttggcttt cttaacaaac agtttaggaa
     9001 aagcagttga aatctctcaa gatgaatggt ttaatgatgc taaggacttg atttatcaat
     9061 ggttcgttta cttgcaaaag caagatactc aagcctttat ctatgtccaa aaaaaattgg
     9121 taaaatcatt taaagaaaaa acacaacagt ttacaggatt atctgtcttg atgttttatt
     9181 atcaggaagc acagcgtcaa gcagtagcaa acggcgaata tggcaaagtg aaacagataa
     9241 accagataat tgaacggata ttgatcgcag agcagaaact acgcagcaat gtcagttttc
     9301 aggctgttgc ggaacagttc gttttgcata cgatttttta gcttgccgca aaggatgatg
     9361 atcaaggagg atggaaatgg tagaagtagt aggtgtccga tatagagatg caggtcatat
     9421 ctattatttt gcaccaggag actctgaata tgtttatagt gacaaggtat tagtcgaatc
     9481 tcagcaatcc ttacatattg ctactgttgt ccttcctaaa aaagagattg atcccaatga
     9541 ccttcctgaa gctatccgtc cgatcttgca taaagcgtca caagctgaat tagaaaaaga
     9601 caaaaaaaac aaaaaagatg cttatgatgc attgaaaatc gcaaaagaaa aagtgcagtt
     9661 gcgtgagttg gaaatgaaaa tggtcaaagc cgaatacaca tttgatcgaa ataaattgat
     9721 tttttctttc acagcggacg ggcgaattga ctttagagaa ttggtaaaag atctggccgc
     9781 tgtatttcgt actcgtattg agcttcgaca aattggtgtc cgcgatgaag caaagataat
     9841 aggcggaatt ggtccatgtg ggcgtccatt atgctgttct tcttttttag gcgatttcgt
     9901 gcctgtatca atcaaaatgg caaaagatca aagtttatct ttgaatccaa caaaaatttc
     9961 cggtttatgc ggacgcttga tgtgctgtct gcaatatgag agcgaagaat acgagacagc
    10021 gaaaaaagaa ctccctgact ttggaaaagt aattgctaca cctgatggta aagggaaagt
    10081 cgtagggttg aatctgctta gccgtatcgt tagtattcgg ctgactggac gggaaaatcc
    10141 tgtcgaatat gaatgggatg aattgaaaga atttatccaa gtaggtgaga tgaatggata
    10201 agagatcatt atacgacgga ttgaatcaat tagaatcaga gttgagaaat acattaacag
    10261 aacttgttga aatgaagcat gctttgcatg agatcgtaga aaaaaataca actttggaga
    10321 tggaaaatca acgtctgcgg gaacacctgc gggaattgaa ccaagttgca caagctccag
    10381 ctgaaaatgt gaagcaggaa ctttctaagt ctcgtatgaa tcttgaaaaa atttatgaag
    10441 aaggattcca tgtctgctac gatttatatg gatcaagaag ggacaatgac gagccttgtg
    10501 cgttttgtct agaagtgatc tatcgcgaaa gcggtcgatg accaataaca agaggagact
    10561 ggagcggaga aatactgctc cagtcttttt attaaggaga ttgtgacaga tgtataatca
    10621 aaaaagtttc aagggaaatc cggtatatgg ggatttatat cttgtaccga cgccaattgg
    10681 aaatttagaa gatatgagct acagaagtgt tcgtatgctg caagaatccg atcttatcgc
    10741 aagtgaggat acgcgtaata cacaaaagtt attgaatcat tttgaaatcc atacacccca
    10801 aaaaagttta catgaacata attataaaga acgtgtccct caattgattg ctttgttaaa
    10861 agaaggaaaa acgattgctc aagtcagtga cgcaggaatg ccttccatca gcgatcctgg
    10921 acatgagcta gttttagcct gcataaaaga agggatttct gtgatctcta tcccaggacc
    10981 tacagctggg atgacggctt tgattgcttc agggctagtg cctcaaccat ttttatttta
    11041 tggcttcctt ccacgtaaga aaaaagaaca gaaagaaaca ttgaaaaagc taaaagaaca
    11101 gacagttacg ctgatttttt atgagtcacc ttatcggatc actgctacat tgacgaattt
    11161 cttagaaatt tttggaaatc gccaagtcgt tttgtgtcgg gaattgacaa aggtccatga
    11221 agaatacctt agaggaagta cggaagagct actcgcttat atcgaagaac atccagtaaa
    11281 gggtgaatgt tgtctgcttg tagaagggaa tttgttcgaa atgccagtga cggaagttga
    11341 tgaagagttg ggaacgttaa aggaacaagt agaagcaaag atcgcttcag gcgaaaaacc
    11401 aaatgctgct atcaaagcag tagcgctgaa aaatgggctg aaaaaacaag aagtctaccg
    11461 tcagtaccat gaattagact gagatagtat aagttgtcta taatgaaaga ctaatgagga
    11521 aatcagctac taacttttca aatctcttga acgcgaacga atgttcttgt ataatgaata
    11581 agaggtgagg atgatggagc tgcaatttcc tttacataat gatacatcaa gaaaaatcat
    11641 tcatattgat atggatgctt ttttcgcttc ggttgaagaa cgagatcatc ctgagtttcg
    11701 cggacatcca ttagtaattg ccagacaccc cagtgacact ggtgggcgtg gagtagtgac
    11761 tacagcaaat tatgaagcaa gaaaattcgg aattcattca gcaatgagtg cacaaaaagc
    11821 gtatgagctt tgtccgcaag ccatctttaa gcctggcgac catcataaat acagcgatat
    11881 ttccaaacaa gtacgggaag tattctatcg ttatacagat attgtggaac cagttagtat
    11941 cgatgaagcg tatcttgatg tgaccgaaaa taaaatcaat tgcaagtcag ccatcaaaat
    12001 cgcccgaatg atccaagcag atatttggga gagtgtccag ctaacatgtt ctgcgggagt
    12061 aagttacaat aagtttttgg ccaaattagc atcagactat caaaaaccta gaggaatcac
    12121 ggtagttgcc cctcaagacg ctgtgccatt tttaaaagct ttgccaattg aaaaatttca
    12181 tggggtaggg aagaaaactg tacctagaat gcatgagcta gggatttata caggaaaaga
    12241 cctatatgaa tgcacagaaa tgatgttgat tcgaaatttt ggaaaaatgg gctattctct
    12301 ttatcgtaaa gtcagaggaa tccatgattc tccagtaaac gttacaagag agcgaaaatc
    12361 tgtgggaaaa gagcatactt atgggcagcc gttgcaaacg gaagaagcag tgctgactca
    12421 gttgcgccaa ctagctgaaa aagtagaaga agcactgaga agagtccaaa aacatgggaa
    12481 aacagtagtt ttgaaggtca ggtacacgga ctattctact gtgaccaaaa gagtgacatt
    12541 gccagaatat atctataaaa aagaagcact cttttatcaa gcaagcctta tatgggagga
    12601 aatcttggga gtagaaaaag gaatccgatt gttagggatt actttgacga atctggaccc
    12661 gatgacttat gaaaacatcg tccttccatt atgggaaaat caagaaatat aaaaacagct
    12721 ttgagagttg aaaccggaaa tccgttttct ctcccaaagc tgtttttata tcaagtcttg
    12781 ctttttgatt tgttcatagg tttctgttgc atccgtacat ttttcccaaa tcacaacttg
    12841 attggattct aaagattttt ggacgtcaat gatccgctgg ttgctacttc cgcgaaattg
    12901 tagttttagg tcacgtttgc ttaattcaaa gcgtccgtca acaaggatat cgatttgcga
    12961 aaggagttct aatttatctt ctgaatcttg tagcaattca tcaaaggtat agcctgtcca
    13021 tgaccagata tcttttgtcg taccgaactc ttttcggata cgtcgtacga cttgcagaca
    13081 aacatctgta ttcaaaaaag gttcgccacc aagtaaagtt aatccttgca cgtaatcgtt
    13141 gcgcaagtca gcgatgattt gttcttctaa ttcaggtgtg aatggtttgc catatcggaa
    13201 attttgtgca gctttgttga agcagccttc gcaggcaaat aagcagccac taacgtaaag
    13261 actgttgcgg acaccttcac catcgacaaa attaaacggt ttgtagtcag caatatagtt
    13321 ttggctgtag tcttttgcta accattcttt tggttttgga tttctcataa aaaaacttct
    13381 ttcatttaag gttcaaagat atagctattg aagataaaat ctactcgacc acataaataa
    13441 gaacaaactg acctaaatcc attgttcaag aaagcttgtt cttattttga tagagggcgt
    13501 tcaaagatac aaagccatcg aaagagggtg gtgaaaaaag acttgtcata gcctcttatt
    13561 ctgaataaac ggtgttcaaa agcttgcatc tgcagtaata agcccaacta aacgaaaaat
    13621 atgaggaact atttttgttt agctattctt attacttgat gcaggacagc tttttcacaa
    13681 cctcatttca tgtgtttcac acgtgaggaa atttctttat ggcggccatg taccatcgga
    13741 cgagcttgcg gatttcctaa atagccacaa gtacgtttga ctacatcaca tgtttttgga
    13801 tcatgatttc cacattctgg acattcgaac cctcgttctg tcggtttgaa atcaccttcg
    13861 aaaccacatt gatagcaatg atcgattggc gtattcgtac caagataccc gattttgtca
    13921 tatgcaaaat cccatacagc ttctaaagct ttcggatttt gttggagcat agggtattca
    13981 cagtagtgga tgaatccacc tgaacaatat tttggataat ccttctcaaa ttccaatttt
    14041 tcaaatggcg ttgggttttt acgcacatca tagtggaaac tgtttgtata atattcttta
    14101 tctgtgatat tttcgatgat gccgaatttt tctgtatcca aacggcagaa acggtcagtt
    14161 agactttcac ttggagttga atagacactg aaatgataac catattcatc cccccatgca
    14221 tctgcattag cttttaattc tttgacgata tcaagtgtga aagcttttgc ttctgggttt
    14281 gtttcccatt cgccaccgaa gaaagctgaa gcaacttcat acaatccaat gtagcctaac
    14341 gatacagtag ctcgacggtt tttgaataat tcattgacag aatcggtacg tgaaagacgt
    14401 ttcccgaaag caccatacat atataaaatt ggcgcatttg ctggaatggc ttctttacaa
    14461 cgttctacac gatagagaag cgcatctttc atgatttcta agcggtcaga cagtaactgc
    14521 cagaatttat tctgatctcc ttttgattcg atcgcgattc ttggcaaatt caatgtaaca
    14581 acacctaaat tcattcgtcc gacatttact tcttgaccat tttcgtcttt ccacccttgc
    14641 aagaatgaac gacagcccat aggtactttg aaacttccag ttaattcaac gattttatcg
    14701 taattcagaa tatcaggata cattcttttc gttgcacatt ccaatgctag ttgtttgatg
    14761 tcgtaattag gatcttcagg gtttaagttc acaccttttt tcaaagtgaa gatcagttta
    14821 gggaagatag ctgtccgctt ttcactccct aatccattga ttcgaatctg taaaattgct
    14881 ttttgaattt ctctttcaaa ccagttttct cctagtccaa atcctaatga agtgaaagga
    14941 gtttgtccat ttgaagtaaa taaggtattg atttcatatt ccaaactttg catagcatcg
    15001 aagatatctt ttttggtttt tgcttttgca tattcttttt ggcgttctgg cgaatcgatc
    15061 cattcttccg cgtcttttag atgtttttta tagttcaatt ctgcaaaagg agcaagcaat
    15121 tcatctgttc ggtcggcaga acagccacca tattgacttg acgctacatt ggcaataatc
    15181 tgtgagattt gtgctgtggc ggtctggata gattttggcg attcgacttc ggcattgccg
    15241 attttgaacc cattgttcag catgcctttg aaatcaatca agcagcaatt cgtcatcggt
    15301 gtataaggat gataatccaa atcatggtaa tggatatcgc ctttttgatg cgcatttgct
    15361 atatgtggtg gcaacatttt cagtccgatt gattttccaa cgatcccggc tgtcagatct
    15421 cgctgtgtat tgaaaacatt actgtcttta tttgcgtttt cgttcacgac agtttggtcc
    15481 ttgttgatta gcttttcaat cgaaaagttg atatctgttg ctttactgcg agcaaaatct
    15541 cgtttggtac gataattgat gtactcttct gctaattcat attcgttttt ttctagtaat
    15601 atatgttcca cgatgttttg aatctcatag attttgacat tttcagcaaa ccggcttttg
    15661 atttcttgga caaccaagtc tgcaatcatt tgaatttttt cataagtaag tggtgataat
    15721 gagccgtgaa ttttcttttc tgctttgatc agtgcttcat tgatcttttg ttcatcgaaa
    15781 tcaacatggc ggccatcgcg tttgatgact ttcatctggg atgtctctgg ctgctgagta
    15841 acgacagttt tttcttcata atttactttc attatcttca actcctgcta aattgttctt
    15901 gtctatcata aaacaaaatc ggggaaggag caactaaata
//
LOCUS       DAKBVG010000049        15793 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000049 DAKBVG010000000
VERSION     DAKBVG010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 15793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.049"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(28..330)
                     /locus_tag="OUD09_001089"
     CDS             complement(28..330)
                     /locus_tag="OUD09_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9692371.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            complement(550..916)
                     /gene="ssrA"
                     /locus_tag="OUD09_001090"
     tmRNA           complement(550..916)
                     /gene="ssrA"
                     /locus_tag="OUD09_001090"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1087..1860)
                     /locus_tag="OUD09_001091"
     CDS             complement(1087..1860)
                     /locus_tag="OUD09_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCT9692372.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            2004..2354
                     /locus_tag="OUD09_001092"
     CDS             2004..2354
                     /locus_tag="OUD09_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HCT9692373.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2335..3051
                     /locus_tag="OUD09_001093"
     CDS             2335..3051
                     /locus_tag="OUD09_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCT9692374.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            3051..4499
                     /locus_tag="OUD09_001094"
     CDS             3051..4499
                     /locus_tag="OUD09_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCT9692375.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4569..5078)
                     /locus_tag="OUD09_001095"
     CDS             complement(4569..5078)
                     /locus_tag="OUD09_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9692376.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(5068..5793)
                     /locus_tag="OUD09_001096"
     CDS             complement(5068..5793)
                     /locus_tag="OUD09_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9692377.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(6016..8136)
                     /locus_tag="OUD09_001097"
     CDS             complement(6016..8136)
                     /locus_tag="OUD09_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HCT9692378.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            8366..9544
                     /locus_tag="OUD09_001098"
     CDS             8366..9544
                     /locus_tag="OUD09_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HCT9692379.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            9560..10318
                     /gene="aroD"
                     /locus_tag="OUD09_001099"
     CDS             9560..10318
                     /gene="aroD"
                     /locus_tag="OUD09_001099"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCT9692380.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            10341..10826
                     /gene="ybaK"
                     /locus_tag="OUD09_001100"
     CDS             10341..10826
                     /gene="ybaK"
                     /locus_tag="OUD09_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HCT9692381.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(10897..12150)
                     /locus_tag="OUD09_001101"
     CDS             complement(10897..12150)
                     /locus_tag="OUD09_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCT9692382.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            12267..13616
                     /gene="gdhA"
                     /locus_tag="OUD09_001102"
     CDS             12267..13616
                     /gene="gdhA"
                     /locus_tag="OUD09_001102"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HCT9692383.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            13728..15074
                     /locus_tag="OUD09_001103"
     CDS             13728..15074
                     /locus_tag="OUD09_001103"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCT9692384.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(15382..15768)
                     /locus_tag="OUD09_001104"
     CDS             complement(15382..15768)
                     /locus_tag="OUD09_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCT9692385.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         5164 a         2826 c         3174 g         4629 t
ORIGIN      
        1 cgggctacct tttttaggta gcctacttta atctttatac ctttctggat caacgaaagt
       61 atacttcaaa tagtcataac gccgatgatc gctccgtgcg tctggcacat cagtcacgat
      121 atcaaacaaa aaatacacat ctttcttcat tctggttttt gttgccggaa ttttaaagta
      181 gttcttatta gagtaataga gattgattaa taagctatct tcgattgcta aaaagaaaac
      241 ttctgaatcc cacaccttat aaaaatcttt gacaaatcta ttcgaaggat caaatttaaa
      301 ccataattgt gtttttcctt ccatcaacat aatgttcacc tcaaaaagag tatacgaact
      361 aatgttcttt ttgtaaagta ggaatatcat tgataatatg gatggaacat atatattaaa
      421 aagtgttctt tttgaatgct attttgaatg ccattttcat tcatttaacg aaaatctgcg
      481 aaaataaaaa atgctcaaac ccttataaaa taaggattta agcatctagc gaaatctgct
      541 gaaatctcac aatggagacg gcgggagtcg aacccgcgtc caaacacatc gccacttaaa
      601 aatctacgtt catagtcaac tcatttaagg attcacttta cagcttgccg agtgacaggc
      661 attctgtatc gctagtctga tgttctcttt ttcaacttac agacggaaag ttgaaacgta
      721 tcccactaaa gttaggaccc ttactcgagc acatgggcga tgccgagagg atctacgcta
      781 actgttttta ggcagctaaa gcgtaagagt tgttttcgtt tttagcagtt atatttaact
      841 gtaacgtttt tacgtagacg taacctacga aacgcaattc aagctcgact gtgcctgtcg
      901 aatccgtaac gtccccgtta gctaacaagg gttacgtgta tttttcatac aaaattagta
      961 tagcatatat tgggataaat tcaataaaaa agcgaatcat atcccattat ttatataata
     1021 actgcccctt tcttaagaaa aaataagcga ttttcttaag aaaagaggta gcttcaaaaa
     1081 aagatcttaa atcagatcaa aatacttcaa tgcgtggaca attccgcctt ctgtattttt
     1141 ctttgtgata aaggtagaaa gttcttttaa tccgtcatag gcgttgccca tagcgatttt
     1201 gttgtcgcaa gcttccaaca aggcaaaatc attcggacca tcgccaaatc caaaagtagg
     1261 aactttttcc agctgaagtt ctttttgcag acgtttgaca cctgcccctt ttgagattcc
     1321 tttcttcact gtatcgatcg agtaaggacc attccgataa aaagttagct caggaaaatg
     1381 ttcgtgatag tattcgtcac ctgattgaga taagatcagc aacatattta ctgtgttttc
     1441 tttaaagcct aaaggatcaa tttccggaac atctgaatga atgaagtcgt aagcattgat
     1501 cattgtttgg ctatgaccgg tacaccagat tttctgatcg ttatagtaag aaagttcatg
     1561 accgttcaac tgtacatgat catacatttt ttgacattct tctgaagaaa atgtttgaga
     1621 atagatttct tcgccgtcta ctcgaatgaa agaaccattc atggtgaccg cagaagtaat
     1681 gcccgcatct tccatgatag agtggatttc tgcttctgta cgtcctgtag cgatgacggg
     1741 gagaacattg ttttctttta atgcgttcat agcttttgcc acttcgggtg tgatttttga
     1801 tttcgcatct agtaatgttc catcaagatc aaagaaagtg attgcacgat aatttgacac
     1861 aaataaaact ccttttttat tggaataagt ttatcttaac aaagaatgaa caaaagacaa
     1921 acctttcgtg aagaattcct gaacatattg gttcagtcaa caatttagag tatactataa
     1981 gaagagaatt taggagggaa agaatgattt ttttcagtat tggtatctta ttcttaataa
     2041 ttggcagtct ttttcggatt ctgccctcaa aaggaaatct gcctttttat gggtatcatt
     2101 cgcctcttgc cgcaaaaaca gacgctcatt ggcggctggc tcaaaagaca agcggaaact
     2161 ggttttttct tatgggctta ctgatggcat tgatcggcta ttatttaaag acatctgggc
     2221 atacgaatta ttttctgatt gaaatgcttc tgctagtatt tccaattatg cctattttta
     2281 ttatgacaga aaagaaatta cagaaatatg acctggaaac tggagggaac gataatgaat
     2341 attttaatga ttgaagataa tcaatctgtt tctgaaatga tgcagatgtt tttcttaaat
     2401 gaaggttggg aagcaacttt caagtatgat ggaaaagaag ggctcgatgc ctttttagaa
     2461 aacccacaac attgggatat gattacacta gacctgaatt tgcccacact ggacggtatg
     2521 ctagtaggaa gagagatccg caaagtttca agtactgtgc cgattatcat gctgacagct
     2581 agagattctg aaagcgatca ggtgatcggc ttagagatgg gggcagatga ttatgtcaca
     2641 aaacctttca gtcctctgac gcttattgct cgaatgaaag ctttgcatcg ccgtagtgag
     2701 ttggcagaag caagagaaga agcagtagtt tcagatgatc attttgatat cgagactagt
     2761 catttcaaga tgaatacgaa aacacgagaa gcgtatctgg acaaccggcc aattgaagga
     2821 ctgacgccta aagagttcga tctattgttt actttagcaa aaaaaccacg tcaagtattc
     2881 tctcgggagc agttgcttga attagtctgg gattatcagt actttggtga tgaacggaca
     2941 gtggacgccc atatcaagaa attacgacaa aaaatcgaaa aggtcggacc acaagtcatc
     3001 caaactgttt ggggagtcgg ctacaaattc gatgattctg gtgttgccta atgcgttatt
     3061 tgtatcagca actcttagct ttctgcggaa tggtcgcgtt gatcttattg atcgttggag
     3121 tttcttttac acaactgaca aaacaaacga ttgaagaaaa caattaccag caactgtttg
     3181 gatatgctga atcagttgaa aagacaacgc aaacctatgc cgatacgctc ccgcagttta
     3241 gtcaagatca ggcgtttcaa aatgcattgc tgctgacaga gcaagtactg acagaacaaa
     3301 atgtgaactt tatatttatc gataaaaacg aacgtgtcat ttatccgact aatacagctt
     3361 tactgaattt taccttaaca gctgatcaat ggaaaaatct gaggaatgga cgtcaagaaa
     3421 aattcacatc gaataaaaat attttaggac aagcacaggc gacctcttac gcacttgtgc
     3481 catttaattt gaatcatgaa ttctatggag cacttgtagt tagtcaacct gcgcgaaata
     3541 tcgataacag cgttcgtcca attacactga acctgttcaa aggatttatt ttttccagta
     3601 tcgttgcagt tattgccagc tatgcttttg cagcattcca agttaagcgt atcaatcggt
     3661 taaggaatgc gacgaaagaa gtgacgaatg gtaattttga tgtacagctc cccgtacatg
     3721 acaaagatga atttgatgag ttagcagatg atttcaataa aatgacgaat tcattgaaag
     3781 aatctcaagc agaaattgaa gaacaagaaa atcgtcgaag acaatttatg gcagatgctt
     3841 ctcacgagat gagaacacct cttacgacaa tcaacggact tctggaaggg ttagaataca
     3901 atgcaatccc tgaaaatcag cgagaaaacg cgattaaatt gatgaaaaat gaaacagagc
     3961 gtctgatccg tttggtgaat gaaaacttgg attatgaaaa aatccgtacg aatcaaatca
     4021 gtatggtcat taaaaaattc aatggaacag aaacattgaa aaatattgtt gcacagttag
     4081 aagctaaagc agaagcagca ggagatacat tgacattgaa agccgttgat gatattgatg
     4141 tttacgctga ttatgaccga tttgtccaaa tcatggtcaa tattattcaa aacgcgatac
     4201 agtttactga aaacggccaa atcacgatta ctttagaaaa aggctatctt gaaacgatca
     4261 tcacgattga agatacggga attggaatgt ctgaacaaca aatgaaaagt atctgggatc
     4321 gttattataa agtagatcct tcaagaaaaa atacaaaata tggtgaatca ggattaggtt
     4381 taccaatcgt gcagcaactt gtacgcttgc ataaaggaaa attagaagta gaaagcgaac
     4441 taggaaaagg aacaaaattc gctgttcgtt tgcctgacgt agaaatcaaa aaagagtaaa
     4501 aaacaagcgg tataaaagat catgaatcga tcttctatac cgcttgtttt tactataaaa
     4561 tgattgattt agcccgcggg ctctaaacga tcaatataag gactttgagc aatagtagca
     4621 tcatgagtaa agagtaagat accttgattt tcttcatgag ctaaataaga aagcaagtgg
     4681 agtagctctt ggatctccaa tacactaagt tcatcaaatg tatcttcaat aacaatattg
     4741 tttcgcttgg aaagcaaggc atgaagtaat tgcagcttta ctttttcaaa agacgtcaac
     4801 tcagctgccg cacaattaag caaggaagga gagatatcaa gtttttcttg ataggccaaa
     4861 cgatcttgtt tgatttcttt ttccggtaca tccaacagga catggtcttt aagattcaga
     4921 taaggaaaca tcggccaatt tttttggatg atcgcaaaat tatccagttt tgcaacaaga
     4981 acctcttttt tcaagtgctc caatatttta tgctgttttt cgagaggaca caaaataacg
     5041 gtcaagtgtt cagcatgaat caaatattca ggtttcatta cgtccaggcc tcctactttg
     5101 caaatgatgg ataaaaaaag tgaaagggaa tagactaaga atgagacagc atccactgag
     5161 cccaataaga gtttgggcaa atcccttaag tatatctgtg aaaaaaagat cattttggaa
     5221 atcgatggta aaaaaagtct gttgatccat cggaattgta agtccgctta ccgaagtcaa
     5281 gatatcacgg atatctggca gttcagtagt ctttaagaca agaaaattga tcctttgaag
     5341 aagcgcttca taaactgact ggaagcttac gatccacaat aaaaccaaga tacaacaaag
     5401 aaagagagga aagaacagct gccagaaaat aaaaaggaat aatttggttt tagaaagacc
     5461 agtcaggtgc caattgatca attcctgctt gttttgcctg actgaatagt aaaagaaaaa
     5521 gataaaaagg aaggcaaata gaaaaaataa actgaatatg ccttttatat aaaatcggct
     5581 agctaattct aaagccggaa gaagctctgt agatgcagaa gcgccaataa atgaaagccg
     5641 atcttggact tgattggcta aagccgattg caaatggtaa agagtcaggc atgctatcaa
     5701 taaaaaagca gaaaagcttg aaaataaaca atagagtatt gtctgctttt tgtaataaat
     5761 taatcgagca gtagcatagc ggattatttt catacttttc acctctataa aaagtataga
     5821 aaatgaatat gaataaagta tgaaattcct ttagtgaaat cacgaattaa aaccagaaaa
     5881 tattttgtaa aaagcaagag gttgtgaaga agctgcccta ttctattacc gtagatgcaa
     5941 gcttttgaac actgtttatt cgaaaagagg agcagcgaca ataattttgt cgctgctcct
     6001 tttaaattta ttttatcatt cggaagaact tgtggtcgtt ccgtgaagtt cttggtacgt
     6061 ttgcgtttta taaagatcaa cagttgattg tccgttgtgt tcactaaaga cactagttga
     6121 cttgtcacca agagattttt cagcttcttt taatttctct aagccattct tgtagtcata
     6181 atcactggta tcaattggat ctaatccact attcgtatag aaccgaagaa gatcaccatt
     6241 attgatttgg tcagaaacag ctaactgttt atcaactttt tctttcagtg aatcaatttc
     6301 attctgtact tcctcagtag gttcagtaat agcttcacca gtttcgttac tatagatcgt
     6361 accaccgtag taagtatatt ttggtgtaac aaaatcacca tcacggaaag aaacaagctg
     6421 ttcatgatct ttcgagaata gatcttgtcc taactgaata taattttttg tatctacacc
     6481 tagtaagtga agtaatgtag gcaaggcatc aacttgtccg ccgtaagtgt gattgattcc
     6541 gccctgtgtt tgtccaggga tatggatcat ataagggaca cgctgcattt gtgcattgtc
     6601 gtagtttgtc caagtagaac ttgtttttcc aactaattca gctagatttt tatttcttga
     6661 attcgagaca ccgtaatggt caccgtaaag gacgatgact gaattatcat acaagccgct
     6721 ttctttcaaa taattgaaga attcttgtac ggcggtatct aaataattgg ctgtcgcaaa
     6781 gtaaccgttg atcgtttcat catttgtatt ggctaacggg aatcctgctt cgtcattggt
     6841 gaattttgaa taaggataat ggttagatac agcaatgaat tttgaataaa atggttgttg
     6901 cagacgttcc aagtattgta cggattgttg gaaaaatggt ttatcatgca acccgtattg
     6961 gaacgaattg tcatcattaa catcataata agaagaatca aagaaataat catatcctaa
     7021 attcttgtat gtctcgtttc gattccagaa tgtaccagcg ttcccatgga ataccgcact
     7081 aatgtaacct tgtgtttgac ttaaaatatc aggtgcagct tggaaagtat tttttccgcc
     7141 taattgtgtg aacaaggagc cttgattcaa gccaaacaat gaattttcca tcagcgtttc
     7201 cgcatcgctt gtttttcctg cttttacttg atggaaaaag ttgtcaaaac tgaacgtgct
     7261 gttgctgtgg taaagactat tgatgaaagg tgtaacttca tgctggagac cattctcatc
     7321 tgtcaattta taatcgatca ggaattgctg gaagctttct aaatggatat aaatcacatt
     7381 ttttcctcta gcgagcccga acaaatcgtc atttggtgct gcataatgtt cttttacata
     7441 attttctact tctttcatat cattgggact tgcttctgcc cgcacttggt ttgtctggta
     7501 agtttgaaca ccgtcataaa ccatgaaagc attcaagcca agatatttga ccaaataatc
     7561 acgagagaat gttcgagtca gcaattctgg acgatcagct tccgccataa aaagatttcc
     7621 tgagaaaatc ataactgcta atgtagagat cgcaaaagcc attcttgctc ggatcgggcg
     7681 agggtccaac ttgatttttt ttgcaatcag cagaacagtc aagataataa aatcgatcca
     7741 atagatcaca tcatatgggc gaaacagtcg aatggcactt tctcccagac cactcgctac
     7801 tttcccagca cctaaaatcg tgttgatcgt aatgaagtcc gtgaattctc gataataagt
     7861 gacattcgaa aaaacaagca atgtcaaaag aaaataaatg accatcatcg tgatataagc
     7921 aactctaggg cgtctgatat aaagagcaat tccaagtaat aacaatgtag ttgcaatagg
     7981 gttgataaac aagataaagt attgaatgcc actttcgatt ccgagatgga aatcaactaa
     8041 ataggcaaaa atcgttttca accaaagtaa aacgactagt aaagaaaaaa atccgagcct
     8101 cttattcaaa aaggcaggtg tttttataca tttcaagata atcttccttt ctgcacttct
     8161 taatcacaga agtgtcctga aaccatgtta cacagctcac ttattctcat ttattttact
     8221 agtatgacaa tatagtgtca attgaaacca gaagtttatg ctgtttcatt aaggttttct
     8281 ttaagcttca tgaaaaaatg agcagtaaga caagtcctat gttttttgtt atacttggga
     8341 agagaaagct aaagcaggtg aaggaatgaa agtaaaagta aaaaaaagag caagcaatcg
     8401 attgaaaaaa ggatatccac tgatccaaaa agaagacctt gctcatcccc aaaaagaaac
     8461 cacgcaatgg gtggaattag ttgattcgca aaaccaattt atcgcaaaag gatatctagg
     8521 aaagcagaat aagggaatcg gttgggtatt gagccagcag aacatcccat ttgatcaatc
     8581 tttttttgaa gaattttttg tgaagtcaaa agaaaaaaga actttctttt tttcagatga
     8641 ttctactacc gcttttcgta tattcaatgg agaaggagac gggatcggcg gtctgattgt
     8701 tgattattat gcaggttatg ctgttttttc ttggtataac gaaacgttat atgcacatcg
     8761 tgaattattg attcaagctt ttcgtgaaat ctacccggaa atcataggag gctatgagaa
     8821 aattcgattt gaatcagtaa atctgcctga gagccagttc atttacggaa aagaggcacc
     8881 ggaacccttg atcgtaaaag aaaacggagt aaaatttgcc gtttatttaa atgaaggatt
     8941 aatgactggg atctttttag accaaaaaga agtacgggga tcattagcag aaggtttcgc
     9001 ttctgggaaa acagttttga acatgtttag ttataccggc gccttttctg ttgctgccgc
     9061 aatgggaggg gcaatcgaaa caacaagtgt cgatctagca aaacgcagtt taaaaaaaac
     9121 acaggaacag tttgaagtga atggtttgga tgtcgatcag caaaaaatca ttgttatgga
     9181 cgtattcgag tatttcaaat acgccaaaag aaaacaattt ttatatgaca tgattgtatt
     9241 ggatccacca agttttgcca gaaataagaa aaaagtcttt cgcgtagcaa aagattatgg
     9301 ggaactggtc aaggatagtc tggatatttt agcagaagac gggatactta ttgcgtctac
     9361 aaatgctgcg aatgtctcga tagaacaatt ccaaacaatg attgaagagg aattccatgc
     9421 caaaaatgtt tcttttgaac aacttgcttt atatcgtctg ccaaaagatt tccagacgac
     9481 ggataccttt ttagaaggca attatctgaa agtgtttgtt tatcaaatta aaaataacca
     9541 ttagaaggag cgatgcagaa tggttcgggt aaagggattg aatataggaa gcggtagacc
     9601 aaagatttgt gcgcctctta tcggggccgt ccgagaggaa gtattacgtg aagcagttct
     9661 tgcacgtgaa gcaggggccg atttagtcga atggcgagtc gatcattatc gtgaagtttt
     9721 ttcttttgat gaagtagcag aaacattggt tcttatccat gagatgcttg atggactgcc
     9781 gttattattt acgtttcgta ccttagctga aggcggagaa caagacatat cgatccgtca
     9841 gtatcgcgac ctttatgaac atgtgataca cacaggatta gtagatatgg tcgatatcga
     9901 gttgttcaaa gtagaaagtc taggaaaaag actgatagag gaaatcaagt ctttaaacat
     9961 tcctttaatt atcagtagtc atgattttaa agaaacacca gcagaccctg tcttgcttta
    10021 ccgattgaat gttatggaac attttggagc cgatatcgga aaactggcag taactcccaa
    10081 taatgaacga gatgttttgc ggttgatgga gttgactaga cgggcaaatg cttttgtctc
    10141 catgcctatt attacgatgt ccatgggaaa tcttggtaaa atttctcgtc tggttggcgg
    10201 aacgacgggt tccgttatga catttggttc cttaagcagc gattctactt ctgctccagg
    10261 acaattaccg gtcaaagcat tgaagcaaat tattcaatta ttaaatgaac cgttgtagaa
    10321 caagtttgag gtgatatttt ttggcaaaga aaaaaatagt aaaaacgaat gcaatccgta
    10381 tggtcgaaca aaaaaagatt ccctatacag agcacgaata tgagtgggac gaaagccacc
    10441 taagtgcgtc atcggtcgct gaacagcttc cagaaagcca atctcgtata tttaaaacac
    10501 tggttgctgt gggcaatgtg acggggcctc ttgttgctgt gataccggga gaagccgaat
    10561 tgaatctaaa aaaattagca aaagtttcag ggaataaaaa agtagaaatg ctccatttaa
    10621 aagatttgga agcaacgacc ggatacatac gtggtggctg ctcgcctata ggtatgaaaa
    10681 aactatttcc tacttattta gatcaaattg cagaaagcta tgaacaaatc attgtctcgg
    10741 ctggcagaag agggctacag atggagttag cacctcaaga tatctgtgca ttaacgagcg
    10801 gacaatttgc agatataaaa caataaatca tacgtataaa agaaagaagg aggccgggat
    10861 gttgtttgtc cagtctcctt ctttcttttt tatacattat gctctttgcc cggcattcat
    10921 tcctgctaca tgccctgtag cgaaagccgc tgtgatatta aagccgccag tataaccatt
    10981 gacatccagt aattctcccg caaaatacag accatcaatg attttactct ccaatgtttt
    11041 aggataaatt tcctttagag aaacccctcc gccagttaca aatgatttct caataggaaa
    11101 agttttgttg attgatagct taaactcttt gcatagttga acaaaatctt gaatttgttt
    11161 ttgagaggct tgttgtcctg tcagatgatc catttcaagc cgctcaagaa agaaaactaa
    11221 caatcgctca ggcaatatac catgccaagc attgaccaag ttctttttgg ttgctttact
    11281 tttttctgtc aatatatgaa taagctcttg tttggtttgt gtagggaaac aatccagaga
    11341 aagtgtcact ggttctcccg ttttctctaa ttctttattg acaaaacttg aacaacgcag
    11401 tgcagccgga ccagaaatac cgaaatgggt gaaaagtaaa tccatcgtat gttcagtcaa
    11461 tacacgacct tttttattta aaacgcgtaa agtgatgtct tgtaaagaaa gcccttgaag
    11521 tgttttctcc tgaatatacg gttcatccga tatcaagggg gactctgtcg cataaagagg
    11581 cgtcaccgtg tgacccactc gcttcacgat cttgtaccca tctccagtag aaccagtaga
    11641 tggataagtc cgtccaccag ttgttaagat aatggcagga gcatggaact cttcgaattc
    11701 cgtgcgtata ccgtaaattt tatgttcttt gtggatcaat ttctctactc gagtagagaa
    11761 tagaagcgtc acatctagtt ctttcagacg attgagcaat gcttcgataa tcgtttttga
    11821 tttatttgtt acaggaaaca tccgtccatg atcctcttct tttaaatgta cgccttgtga
    11881 ttcaaagaag ttcatgatat cgaaattatt ccattgcgca aacgtactat ataaaaactt
    11941 tccatttcca ggaatatgtg caatcaaatc atcaacagga cgattgttcg taacattgca
    12001 ccgaccacca cctgtcatca gtagtttttt tcctgctttt ttgttttttt cgatcaagag
    12061 tacgcgtgcg ccttgttcag ctgcactgat cgctgccatc atcccgcttg ttccagcgcc
    12121 gactacgatc acatcataca gtttttccat tccctcacct caaggaaagt gtagcatatc
    12181 tgaagaaaga aaacagaaaa aaatgttttg agccaaaaga gaaaatgagc taaaatgagg
    12241 gaagataagc aaaggagcgt ggaacgatga caaacgcaac agaatatgtt caagcaattc
    12301 aagaaaagct acatcaaaag gatcaagggc aagaagaatt tttacaagca atcgatgaat
    12361 tcatgcccac ggttatgtct tttttggaca ctcatcccga atatatcgaa aagaatatct
    12421 tagaactttt aacggaacca gaaagagtta ttcaatttcg agtgccttgg caagatgatg
    12481 aaggaaactg gcgagtaaat agagggtacc gtatccaata caactcagct atcggccctt
    12541 ataaaggtgg attgcgcttc catcctagtg tcaatctaag tatcttgaaa tttttggctt
    12601 ttgaacagat tttcaagaat agtttaaccg gtctgccaat cggtggagga aaaggcggaa
    12661 gtgacttcga tcctaaagga aaatctgata atgagatcat gcgtttttgt caaagtttca
    12721 tgctggaatt gtcaaaacat atcggcccat cgattgatgt tcctgcggga gatatcggtg
    12781 taggagctag agaaatcggc tatttgttcg gcgaatataa acgattgaaa aaatatgata
    12841 ctggtgtgtt gacaggtaaa cctttggatt tttggggaag taagatacgt acggaagcta
    12901 ctggttatgg attagtgtat tatgtcaaac atctattgaa cgaagaaaaa gattcattta
    12961 atcaaaaaac agtttttgtt tccggaagcg ggaatgtagc catttatgca atcgaaaaag
    13021 tgcaagagct aggcgggaaa gttgttacat gttcagattc tagcgggtac atctatgatc
    13081 cagaaggaat agatgtgaaa ctattgaaag aagtcaaaga aatcaataga caacgtttga
    13141 cggaatacgc aaaccaacgt ccttctgcta tttatcatgc tggagaatct gtttggacat
    13201 tgaaagaaaa agcggatatc gctttgccat gtgctactca aaatgaaata gacgaaagct
    13261 tggccgaaat cttagtagaa aatgggatca aaatcgtagc agaaggcgct aatatgccat
    13321 gtacagtaaa agctgtttct gttcttcatg aagcaggcgt gtggtattgt ccaggaaaag
    13381 ctgctaatgc tggaggagta gcagtatctg ctttagaaat gagtcagaac gtccagcgat
    13441 tgacttggga aagagagcaa gtggatgagc agctggatca aatcatggaa acgatttacc
    13501 atacgtgtcg ccaaacagca gccgagtacg gcgacgaaaa aaatctgctt cttggagcaa
    13561 atgttgctgg atttgaaaaa gttgccaaag caatggcagc agaaggttta gtttgaaaaa
    13621 tgaaactttc ttggaaatat tgtcagcatt ttgtttattt gcttgaaaaa taataaaaaa
    13681 aaacgtaaag tattacctgt agagtaaacc tataaggagg agaacaaatg gcacacattc
    13741 attttgacta ttcaaaagta gcaccgtttg tcaacgaaca agagttaggg tacatgcaaa
    13801 gccaagtaac agcagcccac aacgaattac gggaaggtac tggcgcagga agcgacttcc
    13861 gcggctggat cgatttacca acgaactatg acaaagaaga atttgcacgt atcaaagaag
    13921 cagctaaaaa aatccaatct gattctgaag tattagttgt tatcggtatc ggtggttctt
    13981 atttaggcgc tcgtgcagcg atcgacttct tgaatcacac attcttcaat ttattagata
    14041 gcaacacaag aaaagcacca caaatcttct ttgccggaaa ttcaatcagc tcaacatata
    14101 ttgctgactt gatcgaagta atcggtgatc gcgacttttc agtaaacgtt atttcaaaat
    14161 ctggaacaac tactgaacca gcgattgcgt tccgtgtatt caaagagctt cttgttaaga
    14221 aatatggaca agaagaagca aacaaacgta tctacgctac aacagataaa gcaaaaggtg
    14281 cagttaaagt agaagcagat gcagaaggtt gggaaacttt cgtgattcct gatgacatcg
    14341 gcggacgttt ctctgtatta actgctgttg gtttgttacc aatcgctgtt agtggtgcag
    14401 atatcgatgc attgatgcaa ggtgccgctg atgcaagcaa agcatactca agcgacaaac
    14461 tagaagaaaa cgaagcttac caatacgcag caatgcgcaa cattctttac cgtaaaggca
    14521 aagtaactga gttgttgatc aactatgaac caggaatgca atacttctca gaatggtgga
    14581 aacaattgta tggtgaatct gaaggaaaag accaaaaagg catctatcct tcaagcgcaa
    14641 acttctcaac tgatttgcac tctttaggac aattcatcca agaaggacgt cgcaatattt
    14701 ttgaaacagt tgtgaaggtt gaaaaacctc gtaaatcaat cacaattcct gaacaagcag
    14761 aagatttaga cggtctaggc tacttgcaag gaaaagaagt ggacttcgta aatacaaaag
    14821 catttgaagg aacattgctt gctcatacag acggtgacgt gcctaacttg ctagtcaaaa
    14881 ttccagaaat ggatgcttat acattgggtt atacaatgta cttctttgaa attgctgtag
    14941 gtatttcagg ttacttgaat ggaatcaacc cattcgacca accaggtgta gaagcttaca
    15001 agaaaaacat gttcgcactt ctaggcaaac caggctttga agatcttgct aaagaattga
    15061 acgaacgcct gtaagataag ggtttttcgc tagatttaaa taatcagtga tagaaacatg
    15121 ttcgctttaa aatgaaaact ggtacccatt taggtactca aacatactgc atcgtttatt
    15181 cagaataagg ggctgtgaca agacgtttgt cacagtccct tgtttgtata gaaatcgatt
    15241 gattgccttg ctctttagtt tgttttagga ctttaagaac agtgaggctg acttcgaagg
    15301 ttaattctgt ttttttgttt tctagaatca gtaagcaaaa aagcactcac ttcgagtgct
    15361 tttttgcgat atattcttag cttaagatag gcctaatttt tcttttacag atgagggtac
    15421 cttatcgctg gtaattactt ttttctctat gatccgcttt tcgcttgctt tcaactgcaa
    15481 gatcgttcct tcggggtaag cagaaccatt ggcagagtca ccagaagtaa tgacatcgaa
    15541 atctaactgt cttttttcac cgttttcagc ataagcatct acgttgtatt taaaacctct
    15601 tccaattact tccccattat ctgctttgat atcagtttct gcaggcattg gagctttgac
    15661 taccgcataa taatctttac ctacataagt agactgatag tagtcccaac ctttccatcc
    15721 tataaaagca atcaatatga taacgatcaa ccctaaaatt tttttcatga aagatctcct
    15781 tttttatttt tat
//
LOCUS       DAKBVG010000050        15572 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000050 DAKBVG010000000
VERSION     DAKBVG010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 15572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.050"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(<1..829)
                     /locus_tag="OUD09_001105"
     CDS             complement(<1..829)
                     /locus_tag="OUD09_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCT9692386.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     "
     gene            complement(1053..2993)
                     /gene="thrS"
                     /locus_tag="OUD09_001106"
     CDS             complement(1053..2993)
                     /gene="thrS"
                     /locus_tag="OUD09_001106"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCT9692387.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3330..3509)
                     /locus_tag="OUD09_001107"
     CDS             complement(3330..3509)
                     /locus_tag="OUD09_001107"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HCT9692388.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3556..5379)
                     /gene="cadA"
                     /locus_tag="OUD09_001108"
     CDS             complement(3556..5379)
                     /gene="cadA"
                     /locus_tag="OUD09_001108"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HCT9692389.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5657..7936
                     /locus_tag="OUD09_001109"
     CDS             5657..7936
                     /locus_tag="OUD09_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCT9692390.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8029..8676)
                     /locus_tag="OUD09_001110"
     CDS             complement(8029..8676)
                     /locus_tag="OUD09_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9692391.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNNVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8855..9574)
                     /locus_tag="OUD09_001111"
     CDS             complement(8855..9574)
                     /locus_tag="OUD09_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCT9692392.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9705..10856)
                     /locus_tag="OUD09_001112"
     CDS             complement(9705..10856)
                     /locus_tag="OUD09_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HCT9692393.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKTTGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTYSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDVGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11073..11813)
                     /locus_tag="OUD09_001113"
     CDS             complement(11073..11813)
                     /locus_tag="OUD09_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCT9692394.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEIFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11817..12173)
                     /gene="rsfS"
                     /locus_tag="OUD09_001114"
     CDS             complement(11817..12173)
                     /gene="rsfS"
                     /locus_tag="OUD09_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCT9692395.1"
                     /translation="MIDSKEQLKIAVEAADSKRAEDIIALDIRNVSLLADYFMICSAN
                     SERQINAITEEIIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERSFYNLE
                     KLWSDAPLVDLSEWLD"
     gene            complement(12197..12790)
                     /gene="yqeK"
                     /locus_tag="OUD09_001115"
     CDS             complement(12197..12790)
                     /gene="yqeK"
                     /locus_tag="OUD09_001115"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HCT9692396.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12774..13424)
                     /locus_tag="OUD09_001116"
     CDS             complement(12774..13424)
                     /locus_tag="OUD09_001116"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCT9692397.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13434..13745)
                     /gene="yhbY"
                     /locus_tag="OUD09_001117"
     CDS             complement(13434..13745)
                     /gene="yhbY"
                     /locus_tag="OUD09_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCT9692398.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13761..14870)
                     /gene="yqeH"
                     /locus_tag="OUD09_001118"
     CDS             complement(13761..14870)
                     /gene="yqeH"
                     /locus_tag="OUD09_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HCT9692399.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNEIQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14867..15394)
                     /locus_tag="OUD09_001119"
     CDS             complement(14867..15394)
                     /locus_tag="OUD09_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HCT9692400.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
BASE COUNT         4440 a         3374 c         2703 g         5055 t
ORIGIN      
        1 attcttggat tgcttctcgt gtttttttca ctttttcgct aagcttttcg tttttagcca
       61 tatagaaata aaaaatgata tcgatcgtag caaattgggc caaaagtgaa ttagttgccg
      121 cacttcgatt cgtgatttct tttggtctag atgtttgtac gagtacatcg gctatcttgc
      181 taagcgggtt atttccaaat tgcgtcaacg caatgatttc gatattcatg cttttcgcca
      241 gctcggctaa tgcgactaca tctgcacttc tacctgagtt tgaaacaagc caaagcactt
      301 tctttttctc attgctgaca agttgcggga gcaagacgtg gatatctttt tcaaaaatga
      361 ttggcttacc gacacgagac catttttgca gaatatcttc tactactaaa gaagaggcgc
      421 caaccccata taaaaaaaca gtctctgccg attccaaaca ctctacgact ttttggatcg
      481 tttcttcttc tagaatatct actgtttctt ttatcgctac ttgtgcattg ttaccaagtt
      541 tgtttttgat agagtaaaag gactcatttg cttctatatc actaaaacct tcatcgattc
      601 cagtctgtag ttctgttgaa atcgtgattt tgaagtccgt cagactttga aaaccgattt
      661 ttttggaaaa acgtacaact gtcggcgcac tcaacccgga agcttctgct aattcattag
      721 ctgtcattgt agggaccttc tcaatattac cgataatgta gttggccaac tttttttctg
      781 ctgaagaata atcttccatc atactttcaa tacgactaat tacgctcata agttcctcca
      841 cccttttaat gatagtctat gtaagtatac catatcagac ccttattcac ggtcgtatac
      901 tggataaaaa acaaaaaaac aatccgccca acagaatatc ttatcccatc aatcccacag
      961 attctattga gcatctgtgt ctttgaggaa tacctgttat ctattgttcg gattgctttc
     1021 tatctactac ttttttacag gaaatttacc tattagtttt ctctgctgta atttttgact
     1081 tctgcttcca tagcatcaat aaagatattc aaatcttcta cagctgtttc tttacttcca
     1141 taacgacgta cgttcactgt tgcatcttcc aattccttgt ctccaacaac taattggtaa
     1201 gggatctttt gtgtttgaga ggcacgtatc ttatagccca ttttttcatt acgatcatca
     1261 acttctacac gcaacccctt catttgcaaa cgttctttga tttcataagc ataatcgcca
     1321 tgtgcatcaa cagaaacagg gatgattgtt gcttgtatag gtgccaacca tgttggaaaa
     1381 gcgcctttgt atacttctgt taaataagcg acaaaacgtt ccattgtaga aacaattccg
     1441 cggtggataa ctactggacg atgcgtattt tccccgtctt ctccaacata tgtcaaatca
     1501 aagcgctctg gcaataagaa gtctaattgt atagttgata atgtttcttc tgtaccaaga
     1561 gctgttttga cttgaacatc tagttttggt ccgtaaaagg ctgcctcgcc ttctgcttca
     1621 aagtaatcta gttctaattc atccatagcg gctttcagca ttgcttgggc tttttcccac
     1681 attgcgtcat catcaaaata tttatctgta ttgtttggat cgcgatagct caagcggaaa
     1741 cgataatctg taatattgaa gtcagcatat actgccacca tcaattcaag cgtacgttta
     1801 aattcatctt tgatttgatc aggacgaaca aatgtatgac catcattcaa cgtcatttct
     1861 cgtacacgtt gtaaacctga caaagcacct gatttttcat agcgatgcat cattcctaat
     1921 tcagcgatac gaattggtaa ttcacgataa gaatggatcg tgtttttgta gaccatcatg
     1981 tgatgagggc agttcattgg gcgaagaaca agcatttcgc catctcccat atccattggc
     2041 gggaacatgt cttcttgata atgatcccag tgtccagatg ttttgtacaa ctcaacatct
     2101 cccattattg gtgtatatac atgttggtaa cctagactaa cttctttgtc tacgatataa
     2161 cgttcgatcg tgcgtcggat tgttgcccct tttggaagcc agaatggcaa accagaacct
     2221 acttctgggg aaaccatgaa caagtcaagt tcttttccta gtttacgatg gtctctttct
     2281 tttgcttctt cacggagacg gatatattct ttcaaagctt tcttgtcaaa aaaggctgtc
     2341 ccgtatacac gttgcatcat ttgattgttt gagtttccac gccaataagc acctgccact
     2401 gataataatt taaatacttg tatacggcca gtggatggaa catgtggtcc acgacacaaa
     2461 tcgatgaatt cgccttgttg ataagcagta atcacttctt cttctggcaa ttcactaatc
     2521 agttctactt tgtacggatc acttgcaaaa atttctaatg cttcctgttt tgaaagaaca
     2581 cggcgttcga tcggcagatt ttctttgacg attttcatca tttctgcttc gattgccggc
     2641 aaatcttctg cagttacttg gttttgtcca ttatctgtat cgtagtaaaa ccctgaatcg
     2701 atagctggtc caacgccaaa gtggatattt ggatagaggc gacgcatggc ttgggccatc
     2761 aaatgagctg ctgaatgacg gactaagcca agtgcatctt catggtctgg tgtgatgatc
     2821 tcaatacttg catcttcctc gattggacga gtcaaatcaa cgagttttcc gttcacttta
     2881 ccagctaaag ctttttttgc taagcttttt gaaatgcttt cagcaattgc caatgttgtt
     2941 gttccagact caaattcttt gactgcacca tctggaaaag taatttttat cataatagtt
     3001 tcctccttga aatgttttga ttgatcagat catcaagctg aaaaacaaaa aaatcctagc
     3061 atctcttgaa aagagatact aggacgaaaa gttcgtggtt ccacctaatg ttcaaacgca
     3121 aatgcgttcc tcaagcgttg taacggtccg accgcccttg cttcaacaag ggtttcgaaa
     3181 gtggtcatcc tttggttttc tcctggaaaa gtttcagcct agcttttcct ctcttatcga
     3241 agactctcaa attcagttgg ctttctcatc aatcaattat gtgtttattt aaccactaat
     3301 aaatagaaaa atcaagtggg aaactaaact tattttttcc attccccatt tacaccgtat
     3361 gttccttttt tcatctcatt gatgatgaca tggatgtgtt cttttggtgc ctgcgtgttt
     3421 ttatgaacag cttccgtgat atctttcatc atattttcta attgttccgg actgcgacct
     3481 tctactaatt cgacatgtac gaatggcata atgctctctc ctctttctga tcgttctttt
     3541 ttcaactcga ttctatcata ctatgtttgt cttgggtact gaatcaggaa aaatggctgg
     3601 atcttttttt gctctcaagg cactcaaaat agagaccgta tccacgactt cttgcaaaac
     3661 agctccgaat aatgcgggga ttagtcccgt acttgcaatt aacatcaaaa aaatacaaat
     3721 aaagatgcca atcagtacag actgtttcgc tattctcatc gtatctcttg aaatcactac
     3781 tgcttttgct acacgcaaga gatcatcttt caagatgacc gcatctgcac tttcactagc
     3841 agctgtagaa ccatgagccc ccattgcaat accgatatct gcagcagcaa gtgctggtgc
     3901 gtcattcacg ccgtctccta ccatcgcaac aggtctttga tctttaggga tactttctaa
     3961 aatggtcagt ttatcttgag gcaagctacg ggcatgtaca gaatctaatt ggactttttc
     4021 tgcaatcgtt tgagcaatgg cagatccatc cccggtgatc atcatcaaat ttttcacatc
     4081 aagttttctt agctccttga ttgtctttgc tgcttctggg cgaatctgat ccgaaaatag
     4141 gatcgttcct gcaaaagatc gatcaattgt tacatagatt gctgtattgg acgtttctat
     4201 ttcttcagct gaaacaaaag aggcacgtcc aatctttact tcatgattat tgactactgc
     4261 ttggattcct tgaccggtca cttctttcag ttgactaaca ggatacagtt ggctgttttt
     4321 cacgttgttc actaacgaac gagctaagat atggctagat tcctgttctg cactagctgc
     4381 atacagaagt agctcttctt tttttattcc attgactggc tgaatcgcct ctacttccaa
     4441 tatccctttg gtaagcgttc ctgttttatc aaaagcaatc gtttttgtac gtgcgagctt
     4501 ttcaatcgtt gttccagttt ttacaacgat cccgttttta cttgaacggc tcattcctgc
     4561 aactaaagca atgggcgcag ctagaatcag tggacaaggt gatgccacta ccagtacctc
     4621 agcaaatcgt aaagggtctt ttgagaacca ccaagaaata ccaccgatca aataagcaat
     4681 caacgtgaaa gggactgcat aacgatcagc taaacggaca aatcgggcag gcttttcttt
     4741 tgattcttcg actagtttaa taatcatttg atactggctg tctgcggcac gtttttcagc
     4801 gatcatttgg atcgatccgt ctccatttaa agaccctgac ataatctgat ctccaggttt
     4861 cttggtttcc gggcgggact cacctgtcaa agaagattca tcgaccgaag attctccttt
     4921 ggtcactcga ccatctgcgg gtacaagttc gccaggcttg actagtaact ggtcatcaat
     4981 atttatttca tcgattacta tatcctgcaa ttttccttct ttgaatcgat gggcaattct
     5041 tggggtgtta tctaacaaag actgcagttc tttacccgct cgtttattcg cgtaatcctc
     5101 tagactatct ccgcccgtaa gcatgatcaa tatcatcaaa ctagcccagt attctttgac
     5161 ggctaatgtt gcaaggattg cggtaatcgc taatacatct acgccatatc ttcctgaacg
     5221 taacgtcttg atcatttcca aaaacatcaa gaaggcgaga atccctccag taaatgcgac
     5281 aatcgcaaat gcaagggccg gctggtgaaa aacaaattca cttaataaag ctatcccacc
     5341 aataagcaaa gtaagtaaaa attttttgaa atgtgtcatt gttttttctc ctcttttatg
     5401 tgacttagag aggttagtaa taaatttcca gctgcctctt tcatcttaag catagtcctt
     5461 gactagcact ttttaaaaca ataggcctta attgaaaaca actatcacta tcagcgtttc
     5521 caaaattcat cagttctaca aacaaaaata ttcagcaagg attacttttt cgattcatca
     5581 gttaaaaagt accttttttc gaaaaactat gctatacttt taattcgtta tttacggcgg
     5641 aaggagcaaa catgcgatga atgagaaaca actagaacaa acgcacgtgg atgaaacgat
     5701 cgaactaatc cagatcgaac aaaatatgct ggatacaaaa caacaagaat tgactcagga
     5761 aatgaatcat tcgacaaaag acacagctaa tcacaagatt cgagctggtt ccaatgaagc
     5821 tttttatgaa tctgctgttg aatatcgcca acatgagcag gaattgcttt tgaaatacca
     5881 tactcttgaa gcacagcaaa aacgcctgca aacgttgtct gtcatgaaag atagtcctta
     5941 ttttgcccgt atcgacttta aagaagaccg tgaaacagaa acattatatc taggtatcgc
     6001 ttctttaaga gatctggcag aagaaacaat cgttattgac tggcgcgcac caattgccaa
     6061 tctttattat gaaggtgaga ttggtcccgc attttatgaa actgaaacag atagaataga
     6121 tgtcgatctg cagttgaaac gacaatttaa aatccaagaa ggacgtattt tatctatggt
     6181 agatacgtcg gaagtaatca atgatgactt tttgctagag atattggatg aatcatcaag
     6241 caaccatatg aaaaacatcg tctctacgat acaaaaagca caaaatgcaa tcattcggga
     6301 cacaacaagt aaagtaatgc tgattgaagg aatcgccgga agtggaaaga catccgcttt
     6361 gcttcaacgt attgcttttc tgttgtatca taatcgcaag tggctcgagg ctgaaaacgt
     6421 tcttctattt tcacccaacc atttgttttc tgattacatc tcgactgtat tgccttcatt
     6481 aggtgaaagc ggcgtgccta cacagacatt caaaaattat ttggtgcagc tgctgccaga
     6541 gttttcttta ctagatgaac agcaacaaga aagtggtttt ttgctagggg aaaaagatcc
     6601 gatacaagtg atgaaaagcg gattgacctt agtcgatcag attgatcgct atatccaagg
     6661 catcacaaac tacggtccct tgttccgtga tatgaaaata aatggccgta cgatcctatc
     6721 aaaggaatcg attcgccaat ggtataaaga aacaaatgaa caattacctt tacatcatag
     6781 actttctcta ttacaaacga agctcctgaa aaaattaggc ggtctgcaaa aagacgaaac
     6841 acgtcagcaa tgggtcaaag atctggcgga agaacagctc caagaactat acgcaacaga
     6901 tccaaatctt gaatatacag aacaaaagga gcgttcatta cgtaaaaaac tggccaacca
     6961 aatcgtacgt aaaagattcc gtcgaatcca tcgagggatc atgaattatc aatttgtcaa
     7021 tataacgaaa caatatctcc actttctaca aactgtgcca ctttctcttc taacacgaca
     7081 taaaatcagc gagaccaggt ggcaggaaca tttgacaact gttcgtacac acttgcgtca
     7141 aagagaattg cgtcaagagg atgctgtact attcttttta ctgatgcgcc gaacatatcc
     7201 agtgacggtt acacacaaag ctcgctatat ctttattgat gaaatgcagg atttcgcacc
     7261 atcacaagtg gcattgctgc aagccttgtt cccaaaagca aatttgactt tctgtggaga
     7321 tctaaatcaa aatgtctttg gaaatgaaac gatcgttgga tcattggaag accttttcgt
     7381 ggaccgtgaa gtcactcgtt tccaattaac gacaagctat cgctccacaa aagaaatcac
     7441 tgattttgct aatcatttta ttgtgaaaga acatcaagta gaaacaacag caagaaaagg
     7501 agaccttcct ttgctcatcc aaggaacaac agatgatatc aaattgaatt ggttagcaga
     7561 taccattcga acgactcagg gaaaagcccg gtattggcgt acagcaatca tagctaaaac
     7621 acaagaagaa tgcgaagctc tttatgctca actgccagac gagttgaaac aacaagtcca
     7681 gctgatttta aatgagagtg actttatgaa gcgttccatt attttacttc cagccttttt
     7741 agcaaaaggt ctggagtttg accgagtttt cttgtgggat gtagatgcag aacatttttc
     7801 ttctcagcaa gacaaattga tcctttacac catgtgtaca cgtgcgatgc atgaattgac
     7861 cctttttacc tccaataatg aaagtccttt gttaaacaat atggatattt catcttatga
     7921 gaaaaaagta ctgtaagtaa aaagacaact gctatcatta atgttagcag ttgtcttttt
     7981 atttacaata ataattgcag aaaaagtaat tcaatcattt aaggtcattt attctatata
     8041 taaatccaaa tctgtttcag gatattttaa tttgacaagc gaaataatca ttttcagttc
     8101 ttccatgttt tctggatatt ctgggtcaaa taccttgcta tgacggccaa tcccgccatt
     8161 gtcgatcaat cggtaaattc gattccattc aatgaattgg tcaattgatt ggaaattgct
     8221 gattggatag ccgccacaat ccaaaatctc tgatacacga aatgctaggt atgcactatc
     8281 gatcacatta ttttttttcg tgtctatctt ctcatttctt tcgataagaa ttttttcagg
     8341 acgatgcttc aagctccact ctaatagtag aaagaaagcc tccatccgat actcttcttc
     8401 aaataaccga aaggctgaat cagaacgacg aaaaagcagc ggaatcagat aattttgtct
     8461 ataaatcagc attggtgcgc ggtccaccat acactctaaa taacggttca gataccctgt
     8521 accgacatag tttcctaatc gatagccttc aacgccgaat cttgcgtatt tttgcgctaa
     8581 ttttgaacaa tcagttacgt tcacagctaa ctcttctact ttgcggtaac cgcttaagat
     8641 acgttcaaaa ggttctaaag tgatttttgc ttccatattc ttccccgtcc atcttccaca
     8701 tacttcatat catttcacat gatacttatg ctctcatcat accacaggtt gaattagaaa
     8761 agaaaataaa tcacacaaaa aagcaagaca atctgttagt gagattgtct tgcttttcga
     8821 aaaagactga atacttttcg ttaaaactgg ttgcttagtc ttctaatgcc acattatgga
     8881 acacatgctg tacatcttct agatcttcca gtgcatcaat cattttttca aatgtttctt
     8941 gatcttctgg agatagtgtt acctcgtttt gagggatcat ttggatctca gcgattgaaa
     9001 aatcagtgat tcctttttct tttaatgctt cttgtatact atggaaatcc gctacttcac
     9061 cgtaaacgat gatttgtcct tcttcttcca ttacatcgcg aacatcgatg tctttttcca
     9121 ttaggtattc aaggatctcg tcagcgtctt ctcctgcaaa agcaaagatt gctgtattgt
     9181 caaacatgta agaaaccgct ccggaaacac ccatgttccc gccatttttc ccaaatgctg
     9241 cccgtacgtc tgctgctgtt cgattcacat tgttcgtcaa tgtatcaaca atcaccattg
     9301 agccgcttgg tccaaatcct tcatagcgca attcagagta tgtttcatct cctgcacctt
     9361 ttgctttttc gattgctcgg tcaataatat gttttggtac attataagtt ttagcgcgat
     9421 caatgacgaa acgtagtttt tggtttgcat gcggatcagg atcacctgat tttgctgccg
     9481 cgtagatttc aatcccaaat tttgcataaa tccggctatt attagcatct ttagcagttt
     9541 tctttgctac gatatttgcc catttacgtc ccatattttc acttcgcttt ctgatttgat
     9601 agtttcataa cgcaaaaacg tcctgttcca ttatacttgt gaatcacgat tctgtgaagc
     9661 tttttttctt tcttccttca ttcttctcat ttttagtcaa ttatctatac tcggattggg
     9721 atttgcccga aattctgttc cggaatattg cctcccaatt gatagatttg atcacttcta
     9781 actaacagtt ccgcttcttg tgcctctttt tttccgattc gggaataaat agaaatctct
     9841 tttttttctt ttaagaatct tcttccaaca tcggtaaatc caagtagtcg aagataattt
     9901 ttattttgtt ggacaggaat ctcttgcttt ttgatattca gcaagacata acaagataat
     9961 cgttggatcc gtgtccatgt atatcttttt gtttttacct ttgacacaaa atcctcaaaa
    10021 ctatcagcag ttgcagctgc tgttttcatg cgcgattcta ttccttcggt catctgatag
    10081 atatctgtca gttccagcaa agtagaagat aacaaccgat aacgcaaata cggccaataa
    10141 gattcccaag tctgcagcga ataagttgaa agatcttctt ctgtctgagg cggcacaagt
    10201 ccagctattc tttttccttc ttctaacgca tggcgaatgg ctgttgcact ggctatcctc
    10261 tgatctgtga tgattttttc atgatagcct gcacttttac gtcggatcgg atgtaattcc
    10321 atagggtgga catacttggc attttccttt gcataactta atcccaagat atgatttggc
    10381 gaagaaaaat caagcgtaat atcgggatac agactgcgaa aaacctcagt cattttttgc
    10441 ggataactat acgtttgatc agacagttct tgaaatgcct cttctatcct atcttgattc
    10501 tcttggacaa agcgtccgaa actgctgtaa tcaaacgaag tctcttcttc tgttccaaaa
    10561 cacaaactgt cacagtccag tgtttgcaag atttttactg cacctttagc aaaatagtca
    10621 gctgactgaa ccgcccattc gacgggaagc tccactacta aatccacacc attttgtaaa
    10681 gcttcattcg cgcgatgcca tttatcgatg atagccggtt cgcctcgttg caaaaaattc
    10741 ccgctcatta cagcaatgac gacctctgct cctgttgttt ttcgtgccat ttccacatga
    10801 tagcgatgtc cattgtgaaa aggattgtac tctacgataa tcccgcagct tttcatgcgc
    10861 atcctccttc atagttatcg ataaaaaaga aatagagaca agtaaaaaaa ggcaaaaatc
    10921 ggcaaaaata cccgaactat ataaactatt ctcgcaaaca caatacttct gcgacgaaaa
    10981 gctgcactgc aagagcaatg cctatatcgt tcggatatat aatttatgtt gaagatttat
    11041 tcttttcttg ttaactcgag caggcaaaac cgctagtctt tgtatgctac aaagaaccag
    11101 cgctttgatt tctctgatgc tgcttcgtct gtaaaatcag aaaatgcttc gactttcgaa
    11161 aagccgctgt tttccagcat tcttaaataa ctttccaatg gataggtacg ctcttgatgt
    11221 agttcatctt cacgaataaa tcgaccagac ccttcttgtt cttcaacgaa aaaggtaaga
    11281 aaatgttcga tgctgtgttt ctcgcttcct ggataactat cccacaaaaa agcaaaatta
    11341 tccccttgat aatgatagct atattctgga aaaatttcat ctacttgata ggtggagtgt
    11401 acgtcaaata aaaatcggcc attttcttct aacgcttgat agacttcatc aaacacttgc
    11461 tgtacctctt gtctattttt catatagcac agcgaatcag aaaaacaggt aattgcctga
    11521 tattgtccga tttctgataa atcgagcata ttgccttcca caaattggat cgatgcgtct
    11581 tcttcatatg ctcgctggct ggcaatcatc aacatctctt ctgaaaggtc taaccctgtc
    11641 actttatatc catcttttgc aaaggcaact gctaatgctc cagtaccgca tgccagttcc
    11701 aaaatatttt ttgtgtcttc ttgtaaatga cgtttcgaaa aagctagcca ttgctgataa
    11761 agtgtttcgt ccatcacttc atcgtaaatg aaagcaaatg tttcataagc catatgttaa
    11821 tccaaccatt cactaagatc gactagcgga gcatctgacc atagtttttc cagattatag
    11881 aaacttcgtt ctggtgcatg gaaaacatgt acgatcacat ctccaagatc gatcaatacc
    11941 cattttccgc cttcttttcc ttcaatacgt tttacttcat atttgttttc ttcttcttta
    12001 tcaatgattt cttctgtaat tgcgttgatt tgacgttcgc tatttgctga acagatcatg
    12061 aaataatctg ccaacaagga aacatttcga atgtctaaag cgataatgtc ttctgcccgt
    12121 tttgaatctg ctgcttcaac cgcaattttt aattgttctt tactatctat gatgatttcc
    12181 tcctaatatg agcagcttat ttttttgcta cccagtaatt atatgtttcg attgtttttg
    12241 gataaatctg ttgttcttgc gcaagcaaat gggccaatgt atgttttgtt tcaaatgcta
    12301 cagcttcgtc tagatcagcc catgcaatgg ctcgtgcatc ttgtacccca ggaaaatctc
    12361 gacctggttc aatatagtct gctacataaa tgattttgtc aaggagactc atcttagcag
    12421 ccccagtcgt atgcacccgg attgcatgaa gaatttctgc atcggtgatt cctaattccc
    12481 gctctacaaa cgaagcgcca acaagaccat gccagattgc attattataa ttcaataact
    12541 ctggatcaaa accgtctctt acgattacca ttttgaactc ctcatctggt cgttcttttg
    12601 cataatcatg tgtcaatgca gcaatactag ctttttcagg agaagcaccg tattttttgg
    12661 caagagccac ggctgtttct tctactccaa gcacatgctt gaagcgttgt tcgctcattc
    12721 tcatctgtat tttttgcatc aggttttctc tagaataagc agtatattca ttactatagt
    12781 ccatattcat acaacccttc attttgaata taatcaatta ctttatctgg aacaaggtag
    12841 cggatagaac agccttcttt gatcttttga cgtattttcg tggaactgat gtcgatctca
    12901 ggtacatcta cccaaattac aggatatgga gtatccgttg tgtatcctgg tcgacgtata
    12961 cccacaaagt tgaccataga agtcagctca tcgattttat accattttgg caaatattct
    13021 accatatccc caccgataat aaagtaataa tctgtatccg gattgttttg cgtcaattcc
    13081 ttcatagtat cataggtgta acttttccca ccacgagcta attcaatcgt ttcaatctgc
    13141 aaaaaaggat tgtcttccac agctaattcc aacatgttga gtcgatgttt tgcatcaatc
    13201 gtctgtttct catcaacatg aggtggctga taagaaggca tcaaaaatac gcggtcaaga
    13261 cctaaattcc ggccagcttg ttcagccatc actaaatgtg caaggtgaac gggattgaat
    13321 gttccgccca agatccctac ttgctttttc ttctctaaga attgaggcat ttcttcagga
    13381 aataattgtg aacgaacaaa tgtcttagct tgcgtattca tcatggtcac tccttatatt
    13441 gcttttactt cttttgatag cctttgatac ttttcttttg tggaaggttt gtataataca
    13501 ataacacgtc caatgatttg gacgatttcg caatctattt ctttttctaa tacttgagct
    13561 gcttcttctg caatctcatc tgtattttga agtaacgata ctttgatcag ttctcttttt
    13621 tctaaagctt cttcaatttg aacgatcatt gctgaattga tcccaccttt tccgatttga
    13681 aaaatcggct gaagatgatg cgctttgcta cgtaaaaaac gcttttgttt tcctcttaaa
    13741 ctcataattt tccttcttcc ttaaatcaat gctttgcgtt tcaatacatc tacacccttt
    13801 ggcgcccagc ccgcaattac tactggcgct gttacagtga tccagcctag accggcaaaa
    13861 acgatgtctg ttttttcttt cacagaaaac tcaaaacgaa caagctccgg aaatgcctcg
    13921 acttcatcag gacgaggagg ctgtaataat ccacctacat gtttttgata aaattcgtct
    13981 gctttttcca gtttcgttcg atgaatattt acatcatttg acacataagc aataaatgaa
    14041 gcacgattgc cgctgataaa atcaaatctt gcaagtccgc ccaagaaaag tgtttgccct
    14101 tcattcagct gataaacttt aggtttgatt tcttttaacg gtgcaatgat ccgcaaatct
    14161 tttttaccta agtagtgcgc catttgatgg cgatggataa ttcccggtgt atcaatcaaa
    14221 aaatgcccgt catctaaagg aatctcgatt ttatccaagg tcgttcctgg aaactgcgaa
    14281 gttgtgatta catcttgtac tcccgctgta tttttgatga tttgattgat aagcgtcgat
    14341 tttccgacat ttgttactcc gacgatatag acatcccgtc catcgcggta ttcttcgatt
    14401 ttttctaata gttccgtcat ctcatgagat ttcttagcac tagttaataa tacatctact
    14461 ggacgaattc ctgcttcgta agcacgttcc ttcatccatt gtgttaattt agtttttttc
    14521 aatgatttag gtaatatatc cactttgttc ccaactaaaa gtactggatt gtcaccgaca
    14581 aaacggtgta gtcctgggat tagtgaacca ttgaagtcaa aaatatccac cacattgacg
    14641 atcaatgcat ctttctgccc taattcattc agtaagcgca aaaagtcgtc atccgttaat
    14701 gagacatctt ggatctcatt ataatggcgt aatctgaaac aacgctgaca atagacttcc
    14761 ccggaagcta aacctttttc caaagccgtt tgcggcgtgt atcctaattc accgggtttg
    14821 tttgtttgta taacggcacc acagccgata cattgtaatt cttctgtcat tcgattccac
    14881 ctcgccagcc catatcaggg tgttttgcta atagataggc cataattctt ttttcccaaa
    14941 aacgattaaa ttgtgtcttc caactatctg ttgcgataat gggttttaca aggatgctac
    15001 gtatccctgc acgattggct ccacgtatat cagtcataat ctgatcgcct accattacta
    15061 tctcatcatc tgctaaatcc aattgtttct ttgctatgtt gatccctctt gctaaaggtt
    15121 tcattgctcg tgcaacatac aaaagatcaa atttttcaat cgctcgcgcg acacgttttg
    15181 aattattgtt tgaaacaaca acaacaggga ttcctgcatt tttcatttct aaaatccaag
    15241 taagcaactc ttctgttcca tctggattat tccatgcaat caacgtattg tctaaatcgg
    15301 tcaatactgc tttgatgcct agattcttta attgggcagg tgtaatctga tagatggctt
    15361 ctaccatcca tgttggtttg taatttgaaa acatgctgtc tcctcatctg caaaaaatga
    15421 aggagcacat aaagtactcc tcaaccccgt attatactgc atatcagcaa aaaaaactac
    15481 ctctatactc gttcgtcttt cagaaaaagc atttctttac aaaatgacca aaaataaaga
    15541 ttgatcacag aaaaatttag aaacaaaaaa ca
//
LOCUS       DAKBVG010000051        14977 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000051 DAKBVG010000000
VERSION     DAKBVG010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14977
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.051"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            85..942
                     /locus_tag="OUD09_001120"
     CDS             85..942
                     /locus_tag="OUD09_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCT9692401.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            1057..1977
                     /gene="pstC"
                     /locus_tag="OUD09_001121"
     CDS             1057..1977
                     /gene="pstC"
                     /locus_tag="OUD09_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HCT9692402.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            1977..2861
                     /gene="pstA"
                     /locus_tag="OUD09_001122"
     CDS             1977..2861
                     /gene="pstA"
                     /locus_tag="OUD09_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCT9692403.1"
                     /translation="MNAKRSDKIATGVLYVVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            2876..3682
                     /gene="pstB"
                     /locus_tag="OUD09_001123"
     CDS             2876..3682
                     /gene="pstB"
                     /locus_tag="OUD09_001123"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HCT9692404.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            3698..4456
                     /locus_tag="OUD09_001124"
     CDS             3698..4456
                     /locus_tag="OUD09_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HCT9692405.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            4469..5146
                     /gene="phoU"
                     /locus_tag="OUD09_001125"
     CDS             4469..5146
                     /gene="phoU"
                     /locus_tag="OUD09_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCT9692406.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            5383..6414
                     /locus_tag="OUD09_001126"
     CDS             5383..6414
                     /locus_tag="OUD09_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HCT9692407.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGRLMNTNQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKHGLFALGSLIINIVLLLLLLLIFTKIEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNLWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            6411..7178
                     /locus_tag="OUD09_001127"
     CDS             6411..7178
                     /locus_tag="OUD09_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HCT9692408.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTTIELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            7278..8846
                     /gene="liaX"
                     /locus_tag="OUD09_001128"
     CDS             7278..8846
                     /gene="liaX"
                     /locus_tag="OUD09_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HCT9692409.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            9143..9463
                     /locus_tag="OUD09_001129"
     CDS             9143..9463
                     /locus_tag="OUD09_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HCT9692410.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            9463..9813
                     /locus_tag="OUD09_001130"
     CDS             9463..9813
                     /locus_tag="OUD09_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCT9692411.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            9980..10918
                     /locus_tag="OUD09_001131"
     CDS             9980..10918
                     /locus_tag="OUD09_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HCT9692412.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            10933..11763
                     /locus_tag="OUD09_001132"
     CDS             10933..11763
                     /locus_tag="OUD09_001132"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCT9692413.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            11807..12832
                     /locus_tag="OUD09_001133"
     CDS             11807..12832
                     /locus_tag="OUD09_001133"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCT9692414.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHCLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPVEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            12847..13785
                     /gene="galU"
                     /locus_tag="OUD09_001134"
     CDS             12847..13785
                     /gene="galU"
                     /locus_tag="OUD09_001134"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCT9692415.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            13910..14350
                     /locus_tag="OUD09_001135"
     CDS             13910..14350
                     /locus_tag="OUD09_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HCT9692416.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            14353..14955
                     /locus_tag="OUD09_001136"
     CDS             14353..14955
                     /locus_tag="OUD09_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCT9692417.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIIIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
BASE COUNT         4927 a         2556 c         3246 g         4248 t
ORIGIN      
        1 ttgttattta atgattttga ttcgttttca tcaagtaatg aaaatgataa aataaacata
       61 ctttaggatt aaaggagatt tctaatgaaa aaacttaaat tattcttacc aataatagga
      121 ttagctattc ttttatcagg ctgttcaaag tggattgatc gaggagaatc cattacggct
      181 gttggctctt ctgctttaca gcctttagta gaaacagttg ctgaaactta ccagacagaa
      241 aatcccggaa aattcatcaa tgtacaaggg ggcggaagcg gaactggttt gtctcaagta
      301 cagtcaggag cagtcgacat cggaaactct gatttgtttg cggaagaaaa atcggggatc
      361 gatgcctcag cattagtcga tcatcgagta gcagttgttg ggatcactcc tattgtgaac
      421 aaggatattg gcgtaaaaga catcagtatg gaaaacctga aaaaactatt tacaggcgag
      481 atcaaaaact ggagtgaagt cggtggaaaa gatcagccag tagttatatt gaaccgtgct
      541 tcaggaagcg gaacccgtag cacgtttgaa aaatgggtat tggacggaga aacagctgtt
      601 caggcacaag aacaagattc aagcggaatg gtgcgccaga tcgttgccga cacacccggt
      661 gcaatcagct atgttgcttt ttcttatgtt tccgatgatg tagatacgtt gaatgttaac
      721 ggtgtagaac cgacagatga aaatgtgaca acgaacaaat ggacgatttg ggcatatgag
      781 catatgtaca caagagatca tccaaaaaaa ctgacaaaag aatttttaga ctatatctta
      841 tctgatgaag tacaagataa catcgttgga gaactaggtt atatccctgt ttctcatatg
      901 aaagtcgaac gtgattggca aggaaatatc gttaaagagt aatctttaca caaaatttac
      961 aaacttagct aaacttttac atgttattaa aaccaaattg atgttcaatt tgtacactga
     1021 gtgagtatgt taaacctgat taagggggag tcatctgtgg aagatgtgaa aaaagtatta
     1081 ttaacaaaat caaaacgttc aaaaatggaa cagcgtggaa aattcatcag ttttttatgt
     1141 atcgcgctga tcgtgttggt tgtattatct attttttatt ttgtagctag taaaggattg
     1201 gccacctttt ttactgataa gatcaatgta tttgattttc tgtttggtac agattggaat
     1261 ccaagccaaa tcggagcaaa tgggaaacca atggtagggg cattgcctat gatcacaggt
     1321 tcgtttattg tgaccttttt atctgctatt gttgccacac catttgccat tggtgcagca
     1381 gtattcatga cggagatctc tcctaaaaac ggaacgaaga tcttacagcc ggtcatcgag
     1441 ctgcttgtag gtattccttc tgtcgtttat ggttttatcg gtttatcagt tatcgtgcct
     1501 tttgtcagaa gtatttttgg cgggacaggg tttggtatct tagctggtac atttgtgttg
     1561 ttcgttatga tcttgccgac tgtcacaacg atgactgttg atgcattaaa ggcagtacca
     1621 cggcactatc gggaagcttc attagcgctt ggtgccacaa gatggcaaac aatctacaaa
     1681 gtcgtgctac gagcagccgt gcccgggatt ttaacagcag tcgtttttgg tatggctcgt
     1741 gcgtttggtg aagccttagc tatccaaatg gtcatcggta atgcagcatt gatgccaaca
     1801 ggacttacta ctcctgcttc tacattgacg tccatcttaa caatgggaat cggaaataca
     1861 atcatgggaa cagttgaaaa taatgtgtta tggtcattag cgctgatctt gctattgatg
     1921 tcattattct ttaatatcgt gatccgtatg attgggaaga aaggagagct gaaataatga
     1981 acgcaaaacg ctcagataaa attgctacag gtgttttata tgtagtttcg gggatcatcg
     2041 tattgatttt agcagcttta ctactttata tcttagttcg gggaataccg cacatttcat
     2101 ggtccttctt gaccgaacct tccaaagcct accaagaagg cggtggaatc gggatccagc
     2161 tatttaactc gatttatttg cttttgatca cgatgatcat cagttttcct atttctttag
     2221 gtgcgggtat ctatctttca gaatatgctg gaaaaaactg gctgacagat gtgattcgca
     2281 cgtcgattga aatcttgagc tcattgcctt ctgtcgttgt cggtttattc ggtttcttgg
     2341 ttttcgttat tcaattccag tatggtttct cgattatttc tggtgcattg gctttgactg
     2401 tattcaactt gcctcttttg actagaacag ttgaggagtc tttacaagcg gtccattaca
     2461 cacaacgaga agcaggattg gctttaggtt tgtcacgctg ggaaacagtg ataaaagtgg
     2521 tggttccaga agcgacacca gggattttga ctggtgtgat cctaagttct ggaagaattt
     2581 ttggtgaagc agctgcattg atctatacgg ctggacaaag tgcacctgcg ttagatttta
     2641 cgaactggaa tccattaagt gtttcaagcc cgatcagcat tttccgtcaa gctgaaacat
     2701 tagctgtcca tatttggaag atcaatacag aaggcacgat gcctgatggt gtttctgttt
     2761 cggctggcgc ttctgccgtg ttgatcattg ctgttttact gttcaacttc ggcgctcgtg
     2821 cgattggcaa acgattatac cgaaaaatga cttcagccta aggagaagaa gcaaaatgaa
     2881 aaaatataac tggaatgaaa caaatgtcat caccatgcca aaagagaaag aagtagcact
     2941 atatacggat gatctacatg tctggtatgg cgacaatgaa gcaatcaaag gagtggatct
     3001 ggaatttgaa aaaaataaga tcactgcact gattggtcct tccggctgcg gaaaatctac
     3061 gtatcttcgt tcgttgaatc gaatgaatga tgggatcgct aatacgaaaa tcactgggaa
     3121 aatcatgtat caaggcgtag atattaatgc gcctcaagtg gatgtttatg agatgcgcaa
     3181 acggattggc atggtttttc aacgtcctaa tccgttcagc aagtcgattt atgacaatat
     3241 tacatttgct ttgaaacagc acggcgaaaa aaacaaaaag aaattagatg aaatcgttga
     3301 aacaagcttg aaacaagctg ccttgtggga ccaagtaaaa gacagcttgg acaaaagtgc
     3361 cttggcttta tctggcgggc aacagcaacg tttatgtatt gctcgtgcga tcgcgatgaa
     3421 acctgatatc ttgctgctag acgagccggc aagtgctttg gaccctattt cgacaggaac
     3481 ggttgaagaa acacttgtaa acttgaaaga agactatacg atcatcatcg tgacgcataa
     3541 catgcaacaa gcggctcgta tcagcgacta tactgccttt ttctatctag gaaaagtaat
     3601 ggaatatgac aaaactaaca agatatttac cagaccaaaa attcaggcaa ccgaagatta
     3661 tgtgtccggt cattttggtt aagacggagg cctcaacatg acaaaagaaa ttatttcatc
     3721 taaagatctt catctgtatt atggaaagaa agaagcacta aaagggatcg atctaagttt
     3781 caatcaaggg gaaatcactg cgatgatcgg tccttctggt tgcgggaaat ccacttatct
     3841 gcgttctttg aaccggatga atgacctgat tccgaatgta acgatcaccg ggagtgtttt
     3901 atataaagac aaagacatct atggtccaaa aacagatgtt gttgaactaa gaaaagaaat
     3961 cggcatggta ttccagcagc caaacccatt tcctttttct atctatgaaa atgtcatcta
     4021 tggattgaaa ttaaaagggg aaaaagataa aaaagtcctt gatcaagtag ttgaagaaag
     4081 cctgaaggca gcgtctgttt gggatgatgt gaaagacaaa ttgcataaaa gtgctttatc
     4141 tttatccggt gggcagcagc agcgtgtatg tatcgcacgt gttttggcag ttaatccgga
     4201 aatcatctta ttggatgaac caacaagtgc gcttgatcct gtttctactg ggaaaatcga
     4261 aagtatgctg ttagaattaa gagagcggta tacgatgatc atcgtgaccc ataatatgtc
     4321 tcaagcttca cgtatttcag ataagacggc attcttttta gatggtcact tgatcgaata
     4381 caatgatacg aagaagatat tcatgaatcc agaaaaacaa gagacagaag attatatttc
     4441 aggtcgattt ggataaggag gaaacggaat gctacgtaca caatttgaag aagaattgct
     4501 gaatctccat aatcagttct acgaaatggg gatgatggtg agcagcgccg tgcacaagtc
     4561 agttcgtgca tacatcaaac acgataaaat cttagcccaa gaagttatcg aaaacgatat
     4621 aaatatcaat aacatggaaa cacgcttgga gaagaaaagt tttgaaatga tcgcattgca
     4681 acagccagtt acaactgatt tgcgtatgat cattacggtc atgaaagcta gctcggattt
     4741 agaacgtatg gccgatcatg cggtgtctgt tgctaaatcc acgattcgcg taaaagggca
     4801 gacgagaata ccggaaatcg aaaaagagat ctccgatatg tccgattatg tgaaaaaaat
     4861 ggtggacaac gtcctagttg cttatgtcaa aacagacgaa aaagacgcac gtacgatcgc
     4921 caatatggat caaagagtca atgactattt caatcgaatc tataatgcaa caatcaaaaa
     4981 tatgcaggaa aatcctgaga cagtcatcag tggaacagac tacttgaatg ttgccagtta
     5041 tctagaacgg atcggagact atgtgacaaa catctgtgaa tggatcgtct acttggctac
     5101 agggaaaatc actgagttaa atacgaacca caatgagatt ttctaaacta aagcggtgat
     5161 tctgccgttt gtttaggaaa agaggttgtg aaatcagcgg ttgtgaaaaa gctgttctgc
     5221 atcaagtaat aagaacagtc aaacaaaaat agctcctcat atttttcatt tggctggact
     5281 tattaccgca gatgcaagct tttgaacact gtttattcgg gaaagggttt gtgacaaaat
     5341 atgtcacaaa cccttttcat catttcatag aaaggataaa gaatgcaaaa aaaaattagc
     5401 atcatcacta tattttttat ttgtgtgctt acttcatggg ttattcagca tcaagctgct
     5461 ttatatgaag tacctgtcat cgagatcgaa aaagtacgaa taaaagagga tcatcaattg
     5521 attacaggaa gactgatgaa cacgaatcaa aaaggagaat cgatttattt atcggttcct
     5581 tatcaaaaaa atcaaataga aacagcaaat ataaaagcga gacaaatgta tctagtccaa
     5641 atgcatggag atgaatggca gctagtagca aaaaaaaatg atggctggct tttcttcttc
     5701 acaagtatct tcattggcgc catgttgctt attggcggaa aacatggtct atttgcttta
     5761 ggaagtctca tcatcaatat cgttttactt ttacttttgt tgctcatctt tactaaaata
     5821 gaagggattt ctctattaaa tatatctatc ctgttcgtcg ttatagggat gattattagc
     5881 atattggctt tagatggatg gaatagaagc agtataatca aaatttcagc tgcagttacg
     5941 agtgtttttt tagcattttt tatttgtctt ttgacaatga atctttggaa agaccaagga
     6001 ctgcgctatg aggaactgaa ttatctgaca cgaccgtatc gttctgtttt tctttctagc
     6061 gtactgattg gaacagctgg aggaacactc gataccgtta tcacagtcat tgcgacatta
     6121 gaagaattga ctaagcgaca accagaaata acagcgaaac agttatggct ttcgggaagg
     6181 caagtgggtc gagatgtgat tggctcgatg accaatatcc ttttgttcgt ctatatcagt
     6241 gggtcagttc catcgcttct tttatattta ggaaatcaat ggtcgttcaa agaaacgata
     6301 gaacagcatt tatcattgga atttttacgc gtcattgctg gcagcttagg aatagtatta
     6361 tcgatcccta tctcggtcct cttttttctg actgtaagga ggctgaaacg atgaatgtac
     6421 ttattatact tgttgggatt ttagcaatca gcgtgctact tattggcggt acgcaaggta
     6481 tgtatattct tttaggctta ttcatcaatt tagggatctt tttcttgttg ttattcggtt
     6541 atcatcagaa gtggccgata ctggcattgt cggtcatagg atttttattg attgctgtgg
     6601 ttatcttatt ttttatcaac ggttataatc taaaaatgcg tgcagctttt gtgagtatac
     6661 tgatcttttt attttgtttc ctactattgc ttcctatcac tgatttttta gcaatccaag
     6721 gttttacaac gatagaacta gaagaattat ctggcttaga taagacttta gcgattgatt
     6781 ttagattact tacgagatcg ttgcttctga ttagtcttag tggtgctgtt ttagatgcga
     6841 gtgtagcaat ttctagtggg acattcgaag tatatcaggc aaatccgcaa ttatcttttt
     6901 accaattgcg ccattctagt ttcgctattg ctaaaaaaat attggcgtca acagtaatga
     6961 cacttctttt tgcattcatg ggaagttctt tagcgttgat cctctggttt attgatttag
     7021 acattccttt tcagcaagtg atcaatgaaa aatcatttgt tctagaatat acgatggcta
     7081 ttcttaccac attgtccgca ctacttgttc ttcctttaac ctgcgtgact acagcatatc
     7141 tctttacaaa aaagaaagaa caactcaaga tggagtaaaa gttacaactt aagtcctatg
     7201 acatttttac tgaatcagtg cataatatgg atgtaaggaa aagcttgcaa acaattttgc
     7261 ttaaggaggc aatttccatg aaagaaagag aacgtatttt agatttagtg aaaaaaggaa
     7321 tcctttcgac tgaagaaggc ttagatttat tagaaagcat ggcaactgaa aaagacgaaa
     7381 agcaaatcaa aaaagaagca gacaaggtaa ctgctagtca taaagaaaaa gatcaggcaa
     7441 gtcaattgat cgataaactt gaaaacggcg aagaagaaat ttctgagcca gttgatccaa
     7501 aggaaagaga aagacaagat caagaaaact tagaaaagat cttagatgaa ctagcaactg
     7561 aagcgaataa aacttcggcg cgtctagatg aagtaaatgc tcaattcgct gacaataagg
     7621 cagcacgcaa tgagaaacag gaagcattga tgcagctgaa tacaaaagaa gaattaggcg
     7681 agttgacaga agaagaatta gcacagcgtc agacgttaga agcagaaatc aaagagttag
     7741 aagctagtgg cgatacactg ttagaagaac aattgaaatt agaagcagaa ttaaaagata
     7801 tccgtaagaa tcaatggtct gagaaaaaag aaacgtttac tgataagttt gaattgccag
     7861 atgattggaa agatcaagca acagatacac tgaatcaagt aggcgaaaaa atgtcagaag
     7921 ctggatcaca gttaggaaaa ttcttgaaga aaacattcca aactgtttca gaaacagtga
     7981 atgacaacat ggaatggaaa gatgtcagct tgcgtgtccc tgggattgcg acaacgaaat
     8041 ttgagcatga attttactat gaagctcctg cagcaagtat ccttgatatc aaggcagcaa
     8101 acggaaacgt gacgttaaaa acgtgggaca gcaacgatgt gaaagttgaa gctaagatca
     8161 aactttacgg aaaaatgggt gctgaaccat ttgaagcatt ctctgaacgg agccagattg
     8221 aggttaatga agatcatatc tccttccaaa ttccaaataa acgtgtgcgt gcagaccttg
     8281 ttttctactt acctaaacga gtatacgacc acgcagctat taaacttcta aatggcaaca
     8341 tcatgattga aacattggaa gctaaagata tttatacaaa atctacgaat ggtaacatca
     8401 tcgtggacca attaactgca acgatgctgg aagtagaagg tgtaaacggc aatatcgata
     8461 ttcgaaacgg gaacatctta gattctatta tcgaaacagt aaatggtaca gtgacatttg
     8521 gtgcgacagc agaaaacctt tctgtgtctt tggtaaatgg tgatgtacgt cttacgatta
     8581 aagaagataa tttgaaaaaa gtagaagcaa gttcagtgaa cggaaatgtc aaagtcgctt
     8641 taccagatgg catcggtttg gaaggacatg ctaaaacaag tcttggaagt atcaacagcc
     8701 gtctgtccaa ttacgaagta gttcgtgaga aaaaagaacg gacaaaccaa atgttgcaat
     8761 tccgtcgtgt ttcagatggt gaaatcgcgc aagtgcaact ttcaacgaca acaggaagta
     8821 tctatttaaa agatacagat aaataaagag gttgtgaaag agatgttcag ccttgagcaa
     8881 taagaagcga ttttgtaaaa tagcttcccc tattttacaa aattgtgcct tattgccgat
     8941 aggctatctc ttgaacaccg tttaccaaga gattatgaac ttgccaatta tcgccgagtc
     9001 acaaggaata atttcaaaaa aagctcgtca cgttttatga atatggtaat ttgtgaccga
     9061 gaattttgca aaaagacacc gattaagaag aaggttgtca caacgatttt cttcatttaa
     9121 aatagtagaa cagaggtgag acatgatgaa aaaaaagttg acaaaatcca ataaaaatgt
     9181 tgttttgaca gggactttag caggaatagg agaatacatc gggattgatc cgactgttat
     9241 ccgtgtgctt tatgtattcc tgagcttagt tgcttttgga agtccaatca ttttatacat
     9301 tttattagct ttgattatcc catctgcgcg ttctgcacga acaaactatg gacataacaa
     9361 tgcatattat gaaaaaaaca attatcgtga acaaactcgt gaacaaacaa aaaaacgcaa
     9421 agaagcggaa aaaatcgatg atgacgaatg gagtgacttt taatgtcgta ctttcaacgt
     9481 attttagtta atacgttaac attcatatca ttggctgttc tttttcctgc gatggtccat
     9541 gtgagcagta tttggacggc cgttcttgcc agcttcgtat tgtctgtgtt gaatatgttt
     9601 gtcaaaccga ttttgacgat actgtccctg ccctttacat tagtcacatt gggattgttt
     9661 acgtttgtta tcaatgcagc gattcttaaa atgacctcct tttttgtggg agcagaacat
     9721 tttagttttt cttcattcgg tgcagcgttg ttagtagcta tcttgatgtc gcttgtcaat
     9781 gtaattgtca gcgaacataa cagtagaaaa taaaagaaag ctgtgccgaa ctatcggcgc
     9841 agttttttta tttagaatca ggaatgcgtt ttctattaca ttggtaatta aataaaaacg
     9901 ttttttttaa ttggtagtac ttttggctag acaatgataa aatgatagag aacactctaa
     9961 tagaaatgag gtagcatgaa tgtcagaagt tgtaaaagtt agccaattag tgaaaaaatt
    10021 atctttggaa atcgtgacag gcgacgagaa aagcttagac cgtgtcatcg taactgggga
    10081 catctcacgc ccaggactag agttgactgg ttattttaat tattattccc ataatcgttt
    10141 acagctattt ggcagtaaag aaatcagttt tgcggaacgg atgatcccag aagagcgttt
    10201 gatcatcatg agaagattat gtagtgaaga tatgcccgca tttattattt caagaggatt
    10261 accagctcca gaagaaatga tccaggcagc aaatgaaaat gggatagctg tactgcgttc
    10321 tcccatctca acttctcgct tattaggaga attatccagt tatttagatg gtcgccttgc
    10381 ccctcgaaca agtgtccacg gtgttttagt ggatgtttac ggattaggtg tactgattca
    10441 aggtgacagt gggataggaa aaagtgaaac agcgttggag ttgatcaaac gcggtcatcg
    10501 attgatcgca gacgatcgag tagatgttta tcagcaagat gaattaacgg tgatcggaga
    10561 accgcctaaa atcctggaac atctgattga aatccgagga atcgggatca ttgatgtaat
    10621 gaatcttttt ggtgcgagtg cggtacgtgg ctctatgcaa gtccagttgg ctgtttatct
    10681 ggaagcatgg gcaaaagata agaaatacga ccgtctgggc tctgaagaca cgaccgttga
    10741 aatcgcggaa gtaggcattc cacaaataaa aatccctgtc aaaacaggac gaaacgttgc
    10801 aatcatcatt gaagtagccg cgatgaattt ccgtgcacgt acaatgggct ttgacgcaac
    10861 gaaaacattt gaagaaagat taagtcgctt gattgaagaa aactcaggca acgactagca
    10921 aggagtgaca atatgttagc acaaatcaat cgagttgctt ttgagcttgt agggattcct
    10981 atctactggt atgcattgat catcgtatca ggtatcatta tcgccatgtg gcttagcagc
    11041 agagaggctg ttcgggtagg attaaaagca gatgatgtga cagattttat gttgtgggga
    11101 ttgcctatct ccattattgg cgctcgctta tattatgttt tatttgatct tccccagtat
    11161 atagctgatc caatccaaat attcaatatc cgttcaggag gcttagctat ttatggcgga
    11221 ctgattgcag gcgcgatcac gctgtatttt tttactaaat atcattttat ttctttgtgg
    11281 acttttttag atatcgctgc accaagtgta ttattagcac aagcaatcgg tcgttgggga
    11341 aatttcatga accacgaagc atatggacca gacacgacgc gaagcttctt agaaagtctg
    11401 catttaccgc aattcatcat tgataatatg tatatcgaag ggatctatcg tcaaccaaca
    11461 tttctttatg aatcactttg gagtttgatt ggatttattt tgcttttgtt tttacgaaag
    11521 aaaccacacc ttttgaaaaa aggtgaagtc cttttatgtt atctaatgtg gtatagtttc
    11581 gggcgtttct ttattgaagg attacgtaca gacagtcttt actttttagg tgtgatccgt
    11641 atttctcaat tattatctgc tgtgttattt gtcggtacat tgattctatt tatttggcgc
    11701 agaaaaaaag gaaatttacc ggattatgac cgctcattag gaaaaaatca acaaattatc
    11761 tgatttttag agcgttaaaa atgaaaaaaa tttcaggagt ggtaaaatga attatagagt
    11821 agcggtttta ggaccaggct cttggggtac tgctttggca caagtcctcg ccgaaaacgg
    11881 acatgacgtt cggatttggg gacatcgtgc ttcacaagta gaagaaatca acacccaaca
    11941 cacgaatcaa cgttatttac cagatcattg tctacccgat tccatccaag cattcaccaa
    12001 tatggaagat gcagtaaaag acgcagatgc ggtgctattc gtcattccaa cgaaagcaat
    12061 acgttctgtt gcaaaagaac tagtgcctaa attgaataca aagccagtga tcatccatgc
    12121 aagtaaagga ttagaacagg ggacacataa aaggatctct gacatcttgt tagaggaaat
    12181 cccagtagaa aaaagaaaag ctgttgttgt tctttctgga ccaagccatg cagaagaagt
    12241 agcgaaacat gacatcacta cgatcacagc tgcaagtaca gatgcaaaag cagcaaaata
    12301 tgtacaagag ctgttcatga ataattattt tcggatctat accaatcctg atgtgatcgg
    12361 tgtagagatg ggggcagctt tgaaaaatat tattgctatt ggtgctggcg ctattcacgg
    12421 cttagggttt ggcgataatg caaaggcagc aatcatgacg cgcggattag ctgagatcag
    12481 ccgtttaggt gtagcaatgg gtgccaaccc gctgaccttt atcggattaa gcggagtagg
    12541 cgatctagtc gttacttgca caagtgttca ttctaggaac tggcgcgcag gcaacttgtt
    12601 aggacaaggg cagcccctag aagaggtgct agccaatatg ggaatggtcg ttgaaggtgt
    12661 gaatactacc caagcagcga tggaactagc ccaaataatg gaaatcgaaa tgcccatcac
    12721 tcaaacaatc tatgaagtgc tgtatgaaca aaaagaaatc aaaacagccg caaaagaaat
    12781 catgttacgt gatgggaaaa aagaaaatga attctcatta acaatttttt gaaggagcga
    12841 atgtatatga aagtcaaaaa agcagttatt ccagcagcag gattaggaac aagattttta
    12901 ccagcaacaa aagctatggc aaaggaaatg ttgccaattg ttgataaacc aacaatccag
    12961 ttcatcgttg aagaagcgtt gaaatcaggt atcgaagaca tcttgatcgt tacaggaaaa
    13021 gctaaacgac caatcgaaga tcactttgat tcaaatattg aattagaaac aaacctaagt
    13081 gaaaaaggaa agaccgaatt attgaaatta gtagaagaaa cgacagatgt caacttgcat
    13141 ttcatccgtc aatctcatcc aaagggttta ggacatgccg ttcttcaagc aagagccttt
    13201 attggaaatg aaccatttgt cgttatgcta ggcgatgata taatggaaga cgaagtacca
    13261 ttgacacagc aattaatcga tgattacgaa cgtactcatg cttctacgat cgccgttatg
    13321 caagttcctc atgatgaaac ttcaaaatac ggaattatca atccaggaga agtattggaa
    13381 gatgggttat acaatgtcaa aaactttgtt gaaaaaccag atccaagcaa agcaccaagt
    13441 gatttagcta ttattggacg ttacttattg actccagaaa tttttgatgt attgtccgag
    13501 caacaaccag gagccggaaa tgaaatccaa ctaactgacg caatcgatac attgaataaa
    13561 acacaacgcg tatttgcccg taaattcaca ggtaaacgtt acgatgtcgg tgacaaattt
    13621 ggttttatga aaacaagtat tgaatatggt ctaacacatc cagaagtagg tgcaccttta
    13681 cgtgagtaca tcctgtcttt aggtaaaaca ttagaagcag aaggaagcaa gatcgatcat
    13741 aatccaaaag gaataacaga aatcaaagaa gaaaagaaaa aataaccctt ttttctaacg
    13801 agcttcagct gctaattgaa taaaaagtag ttgaagctcg tttttatttg tttaaaaaac
    13861 acgaaaatgc tatccttata gaaaagagag acaagaaggg agtttgatca tgtcaggagg
    13921 agaaattgca ggattgatcg cagcaatcgc ctttgcgata cttgtcgttt ttatcgttcg
    13981 tgtgttgttg caagtttcta aaactttaga aaaagtagat cagactgtag cagaagcaaa
    14041 tacgacgatc gaaatcgtca caaaagacgt agatcttttg tctagacaag tagaaggctt
    14101 actggtcaaa agtaatgaac tgctcttgga tatcaatgga aaagtcgcaa cgatcgatcc
    14161 attgttcaca gcagttgctg atttaagtga aagtgtatca gaacttaatc attctagtaa
    14221 aaacatcgcg acgaaagtcg gcggaatcgg taaaacgaca gctaaagcga cggttgccgg
    14281 aaaagtcggt gggacagcga tgaaattttt taaaaacaaa aaacatactg aaacgacagg
    14341 aggaaaataa aaatggctaa aaaaggtgga tttttattag gtgcagtagt cggaggcaca
    14401 gccgcagcaa tcgccgcgtt attgttagca ccaaaatctg gtaaagaact tcgggatgaa
    14461 ttagctgccc aagcggatga tttgatggat atggcttcag attacacaga tatggcgaaa
    14521 gaaaaatcga ctgagttaac aggtattgca aaagaaaaag cgcaagatct ttctcaacaa
    14581 gcaggtgatc tagcaggtag tgtcaaacaa aaagtaagtg aatcagcaga tgaagcagga
    14641 agtgaatctg acgatatctt gttaaatctt aaagaacaat cagctgactt atctaatcgt
    14701 ttcaaaaaaa cggcagaaga tatgaaaaaa cctgcagatg aattaggaag tattgctaaa
    14761 gatacttcag aagatatcat aattgatgta aaagacgctg ctggtaaagc aggagatact
    14821 gtcaatgaag gaatgtcaga agcaaaacca gttgcagatg actcaaaaga aatgatgaaa
    14881 atggcaaaag aagatgcgaa agaagctaaa aaagaaataa aagatacaat gacagattca
    14941 tctacaaatc actaaaatca caaatttata gaataaa
//
LOCUS       DAKBVG010000052        14777 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000052 DAKBVG010000000
VERSION     DAKBVG010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14777
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.052"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            165..1904
                     /gene="yfcC"
                     /locus_tag="OUD09_001137"
     CDS             165..1904
                     /gene="yfcC"
                     /locus_tag="OUD09_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCT9692418.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2061..3245
                     /gene="iadA"
                     /locus_tag="OUD09_001138"
     CDS             2061..3245
                     /gene="iadA"
                     /locus_tag="OUD09_001138"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCT9692419.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3356..3559
                     /locus_tag="OUD09_001139"
     CDS             3356..3559
                     /locus_tag="OUD09_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCT9692420.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3660..4322)
                     /locus_tag="OUD09_001140"
     CDS             complement(3660..4322)
                     /locus_tag="OUD09_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT9692421.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4335..5837)
                     /locus_tag="OUD09_001141"
     CDS             complement(4335..5837)
                     /locus_tag="OUD09_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT9692422.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6385..8064
                     /locus_tag="OUD09_001142"
     CDS             6385..8064
                     /locus_tag="OUD09_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HCT9692423.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8191..8670
                     /locus_tag="OUD09_001143"
     CDS             8191..8670
                     /locus_tag="OUD09_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HCT9692424.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8889..9569
                     /locus_tag="OUD09_001144"
     CDS             8889..9569
                     /locus_tag="OUD09_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCT9692425.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9577..10908
                     /locus_tag="OUD09_001145"
     CDS             9577..10908
                     /locus_tag="OUD09_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCT9692426.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10923..11885
                     /locus_tag="OUD09_001146"
     CDS             10923..11885
                     /locus_tag="OUD09_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HCT9692427.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11996..12334
                     /locus_tag="OUD09_001147"
     CDS             11996..12334
                     /locus_tag="OUD09_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HCT9692428.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12413..13120
                     /locus_tag="OUD09_001148"
     CDS             12413..13120
                     /locus_tag="OUD09_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HCT9692429.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(13223..14704)
                     /locus_tag="OUD09_001149"
     CDS             complement(13223..14704)
                     /locus_tag="OUD09_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCT9692430.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         4710 a         2540 c         3169 g         4358 t
ORIGIN      
        1 gaaagagaaa aaaataatca tacataggag aaaaaaaaca aaatagaaaa aagtgatttt
       61 ctaataaatt attcatgtgg aaatgtcatt tttactgtat aattcatttg attatccatt
      121 ttttattcat caaaattact gaaaaaagaa aggaagaaat aaatttgaag aaaacaaatt
      181 cttcaggaaa accgaaacgc gacttcacta aattatcgac accccatacg tatgtcatta
      241 tttttggtgt agtcatcttt gcatggatac tgacttttgt cgttccagca ggaaagttca
      301 gcacgcaaga tattgaatac aaagatgcaa atggcgaaac aagtactcgt actgttttac
      361 gtcaagattc cttccgttat gcttacgagc tggataagtc gtatgtgttt gatcaattag
      421 aagaactgca agatcatcca gcagaacgtg aaaaactgga tgttccggaa aaaggattgg
      481 aaaaagtcat tgctgacgga gaaaaaaatc ttacgcagga aaaactagat gaaatctcct
      541 tgacagatga tgtgctttac gatcagtacg gcgaaaatat ctatgatact tctaaaaaat
      601 tgcataaaac agccaaaata tggggaacag atgattttgg cggctttggt ttcttaaatt
      661 ttgtttttga aggactagta tctggagata agtatggatc tgctgtaggg atcgctgcat
      721 tgatccttgt tgttggaggt gcttttggta ttattatgcg taccggagcg atcgacgcag
      781 gtatctatgc gtttatcagt aaaactaagg gactggagcg tttagcttta ccgctattat
      841 tctttgcttt ttcattcggt ggcgctacat tcgggatggc agaagaggta attccattct
      901 caatggtgat ggttccattt gtcattgcgt taggatatga ctcgatcgta gcagtaaccg
      961 tgacttatgt cgcatctcaa gtcggaaatg cgacctcttg gatgagcccg tttagcgtag
     1021 cggtggctca agggattgct ggaatccccg tgttgtctgg tgcgacattc cgattgatta
     1081 tgtgggtagt tgttactgca ttagccgctg gttatatgat gatatatgca gaaaaaatcc
     1141 gtaaaaaacc agaaagttcg ttgacttaca aatcagatga ttatttccgt tctcatatca
     1201 aaaagacatc tgatgaaaat aaaaaattca tgcttgggca taaattgatc ttattagaga
     1261 tgttagttgt ccttgtctgg atcgtttggg gcgtgactca aaaaggctat tacattccag
     1321 aaatcgcttc tcaattcttt gtgatgggat tagttgctgg aatcattgct gttcttttca
     1381 aattagatgg aatgaaagtc aatgacatcg cttcttcttt ccaatctggt gcagctgatt
     1441 tagctggtac agcaatcgta gtaggtatgg ccaaagggat tcttcttgtc ttgggaggat
     1501 ctgatgcatc tgttccttca gctttgaata cgattttgca tgggatcggg acggcgttga
     1561 ccggtgttcc agctgtcatt ggtgcttggg cgatgtacat cttccaaagt ttgttcaact
     1621 tggttgttac atcaaattca ggacaagcag ctttgactat gccaatcatg gctccactgg
     1681 cagatttagt aggtgtatcc agacaagtag cagttcttgc ttatcagctt ggttcaggtt
     1741 ttatggacgc ttttacaccc gtatcagcca gcttgattgg tgtgctaggt gttgcacgga
     1801 tcgaatgggc aaaatgggca agattccaaa tcaagatgca aggattcctg tttgttttag
     1861 gaactatttt catcatgatt gccattgcaa taggtctgca gtagtactgg agacctgtaa
     1921 ataaaaaagt aatccaaaca gtagataagg actccaagaa ccgtcatttt acggtaaaat
     1981 caaaggagta aagatcctat cgtttggatt attttttaag atcctatcat cagttatact
     2041 taaagaaaga ggaaaaatgt atgaaaatca ttcgacaaat tgaagtgttt gcaccagatt
     2101 acttaggaaa aatggatgta ctgattgccg gtgggaagat tttggctgta gaagaacagc
     2161 tagaaggcgg ctacgaagga gtggaagtag aagaactttc aggtgaggga aaagtactaa
     2221 ccccgggttt cattgactgt catttccata ttttaggtgg aggaggagaa ggcggttatc
     2281 aaaatcgaac gccagaagtc acattgagcc aattgactac agctggtgta acgacagttg
     2341 tcggttgttt gggaacagac ggagaaggta gagatatgac tgcgttgatc agcaaagcga
     2401 aaggtctgga agcagaagga atctccactt atgtatacga aggttcgtac cgattgccgg
     2461 taaaaacagt aacagattct atcatcaaag actttttgac aattgataaa attatcggaa
     2521 tcggagaaat cgcagtatcc gatcaccgtt cttctcagcc aagttttgaa gagttcgctc
     2581 gtgcagtagc agatgctcgt gtaggaggca tgttatccgg aaaagctggg atcatcaatg
     2641 tccacttagg cggaggaaaa agaaagctag agctattgac acgaatcgta gaagaaacag
     2701 aaattccgat cacacaattt ttgcctactc atgcaaatcg cacaccagaa ttatttgaag
     2761 cttgcgtggc atttgccaaa cgtggtggca cgattgactt cactgctagc gaagatcctg
     2821 atttttggga gaagacagat ggtgaagttc gcttcagcaa agcactgaaa cgattgatcg
     2881 aggaagatgt ttctttagat aatttcacga tgacctcaga tggacaagga agcttgcctt
     2941 attttgatga aaacaatcat ttcctagggt taggagtagg aagtgccaag gcattgctag
     3001 tagggatcaa agaagctgtg caaaaagaaa gtatcccttt ggaaatcgcc ttacgagcta
     3061 taaccagtaa tccagcacgt atattgaaat tggataaaaa aggaaaaatc gaaattggtg
     3121 ctgatgcaga tctgtgtatc ttggataaag aaacgcttga tattgataca gttatcgcta
     3181 aaggagaaat aatggtccaa gaaaaagaag tcaaagtatg ggggactttc gaaaaaagtt
     3241 tttaatcaac agctctaact atctttttta gaaaacgagc gttctgtttg tctgtatctg
     3301 ttgttgatgt taccctgaag gtagatcaat aaacaaacta agaggaggaa gcacaatggc
     3361 agatttaaaa ggacgttcta aagatatgaa agataaagta gtcggtgaag gaaaagaagc
     3421 atacggcaaa gcaacagacg acaagagcaa agaaacagaa gggaaagcac aatcactagg
     3481 tgctgacatc aaagaaaaag ctcgaaattt aggcgacgaa gtcaaagaca agttttctcg
     3541 aaaagacgaa aaaaaataaa aaccacttat tactaattga atcaagcaaa taaaaaatcc
     3601 gaaccatatc tgcgagacag atatggttcg gattttttat ttgatcatta tcctgttttt
     3661 taaacttcga ttttttgaaa ttttttattt tttagttcaa agacttcatc acagcttttt
     3721 gctacttcaa tctcatgagt cacgatgata atacagcgat tttgttcatg ggcgatttct
     3781 tggaaaagtt tgacgatatc ttgtgaagtt gtttcatcta gatttccagt aggctcatcc
     3841 gctacgatta gttgatgttc acagcagata gcacgaacga tagcgactcg ctgctgctga
     3901 cctcccgaaa gtttggtcac ctgttttttt gctaatattt catcaattcc tacacgagcc
     3961 agtcgatcta aagcaacttg tcgataattc tcttgcttgg tcttagtgat ttccattgct
     4021 gctatgacgt tgtctagagc cgataaataa ggaagcaagt tgtaagcttg gaaaataatc
     4081 gagacatcat ttttacgata acttcttaac ccgattttgt tcagtgaacg atcttgatag
     4141 tatacctttc cttcttttgg tgcatctagt ccagaaataa gcgaaagaaa agtcgttttt
     4201 ccagaaccac ttgctcctaa aatagcgtac atttttccag cttcgaaatt cagttctaca
     4261 tctttataaa gcgcatcttg cggatcttga taccaaaatc ccaattgttc tgtccgtaac
     4321 atcaaattcc tcctttagtt taataggatt tttcttgggt ttaatcttaa tatccctata
     4381 gaagctaaaa agacagaaaa cagactgatt ccaaatgcta ctccggctaa tagtcccaat
     4441 tgtgcaggtt tgacattgat ttccaattcg ctgacttgat cagaaacagc aaatggatta
     4501 ttcgtggttc cttgttgacc atcgcgttga ccgccttgtt cttgtctgtt acctggcatt
     4561 tgtccaccgc caggagcttg ctggttttgt gtcgtggttt ctgtctgttg gtcaagtagc
     4621 tgctgtccca cggcattcgc gacgacgttt ccgctaagcg aagcaactcc taatgcaaga
     4681 atcatacaaa tagccacttc ggtaaacaat tggagaatga ttttacttct ggattctcct
     4741 aaagatagca agacgcctaa ctcatgccga cgctcccgaa tggtaatcat aatgatcaga
     4801 gtaagtatga taataccggc tacagccact agtaaaacaa catttttaga aaaacttgcc
     4861 acgttgttta aaggttccaa cattgactga tacatggaat catttgattg taagctaaat
     4921 gtatctgtgt cgatcagctt ctcagcttct ttgagaaact catccatttt atctggatca
     4981 gatagagtgt aaatagcaga atctatcgtg ttatcgtcag atgtcccatt tagttcattt
     5041 gcaaaagtgt aagaagtgta aagtgtattc gacggattca tgaagttgaa tttcataccc
     5101 atactgccgc ttgtttcgct ggattcataa attcctttga tcgtcatttc ataagtggta
     5161 tcttcatctt cagtactcgt gattgtgaaa gtatctccga ctgccaggtc atttgtttct
     5221 gctagagtag aatcaatgac tacattgttt gtgcctttgt cgctgtcatc tattccttca
     5281 ccatccgtga tttttgctgt tccatcagaa aaattactat tttgtgcagt tgctgacaca
     5341 ccactgattt gaaagtctgc ttgagtcatt tgcggattcc cgcccattcc tccagcaggt
     5401 tcaccgcctg catcagtagt ttctgaactg tcttcacttg aatcagaact agtgatggca
     5461 gttattccat cttttgccat agctgaggcg gaactttcaa aggaataact ctttacatta
     5521 tctaattcag ctattttttc cgcgtccgtt aaggaaactg gtgtaagaga aaaactgcct
     5581 ggatctggac gttcttcagt ggtagaaccg cttgaagaag tttgttcctg ttttttaaat
     5641 gaagcttctc gattggcact taaagtcact gttgctccaa cttctttttg tgcttgattc
     5701 gctgcttctt ctgcagcgct tcgaatcgtg aggccagcta gcacaaaaat aagaatggct
     5761 gtgaacacgg caaacaataa caatgaacgt ccccatttta cttttgtact tttccatgca
     5821 cgtttcataa aattcatatt ttgcatccct cctagtagat gcatttgata atagacaacc
     5881 tatcttaaag ctagcttaaa tcttatgctg tttgcacacc atcaacatga ggttctgtat
     5941 atataaaaag tcactatagt tattgcaaaa aatgtggtaa aatgaaaaaa gtatgtttca
     6001 aaccgacgaa gaagaaatga aagaggaata gcaaaagaaa tcctagatgc tacagaggga
     6061 tttttacgtg catagttgtt ctaacaattg ctgatactag cgatttagtt tatttgttgt
     6121 ataataatgg ggaacagctg aatacttata taagtattct ggctatagcg tttcgcaaaa
     6181 gatccatttt gttaatgaag tatatccaaa ataaaatgcc catccaactg tcagaacgcg
     6241 gatgtgctgg taattttcct gctgattctc cataagttga gaaatagtga ttttgcatat
     6301 gcttcattgg aatggctcat atatttagat aaagaagaaa atcgacatct ttaaacttgg
     6361 atgtcataag aagaggtgaa gtttgtgagt accattaaaa ttatcccatt aggcggcgtt
     6421 cgtgaaaatg gaaaaaacat gtatgtcgca gaagtgggcg aggatatttt tattttagat
     6481 tgtgggctaa aatatcctga aaatgagttg ttggggatcg acgtcgtaat ccctgatttt
     6541 acgtatttag aagaaaacat tgatcgtgta gctggtgtct ttttaacaca tggacatgca
     6601 gatgcgatcg gggcacttcc ttacttgttg tcaaaaattt ctgtaccagt attcgggaca
     6661 gagttgacga tcgagttagc aaaattgaat gtcagtcgta atgaagcagc caaaggattt
     6721 aaggatttcc atgtggtaga tgagcataca gaaatagact ttggtgaaac agtagtgagc
     6781 tttttccgta ccactcatac gataccagat tcagtgggga tcaatctgaa aacaaaagaa
     6841 ggtagtatcg tttatacggg agattttaaa tttgaccaaa cggctatccc aatgtatcaa
     6901 acagattatg cacgattggc ggaaatcggc aaagaaggag tcttagcctt actaagtgaa
     6961 tcggcaaatg cagaaaatcc tcagcctgta gcttctgaag cccaaatagc agatgaagta
     7021 tttgatacga tcaaatattg ggaagggcgt atcatcgtcg cttgtgtagc aagtaatttg
     7081 caacgagtgc aacaagtatt ggatgcagca gaaaaagcac aacgaaaagt tgtcttgact
     7141 ggtcaagatt tcgaacgaat tattcgcaca gcgatgcgtt tagaaaaact tcaattacca
     7201 agtgaagatc tgcttgttac gttgaaagac atgaaaaact atgatccaga agaactgatc
     7261 attttagaaa ctggccgaat gggtgaaccc atcaagtctc tgcaaaaaat ggcaaacggt
     7321 acacaccgta atgtcaagat tgaagaagga gatttggttt atatcactac tacgccaagc
     7381 atcgcgatgg aaaccatcgt ggcgaaaacg gaagatatca tttatcgagc tggtggaaca
     7441 gtgaagttga tctctgagaa tatgcgcgtt tctggccatg ccaatcctaa tgacttgcaa
     7501 ttaatgatca atttgatgaa accaacatat tttgtgccag ttcaaggaga gtatcgtgaa
     7561 ttagctgctc atgcagattt ggctcatgca gtagggatgc catacaaaaa tatctatatt
     7621 actggacgcg gagacgtgct ggaatataag aacaagcgta tgagtgttgc aggaactaca
     7681 agtgcagaaa atgtaatgat tgatggatta ggcataggtg atatcggaaa cattgtttta
     7741 cgtgatcgaa aaatcttgtc agaagacggg attttcgtag cggttgtcac tatcagtcgg
     7801 cgagaaaaga aaatcatttc aaaacctcaa attacttcac ggggattcgt ttacgtaaaa
     7861 gctagccgtg atctgatgaa agaaagcagc aatatgattg aaacaatcgt tgagaaacat
     7921 ttagaaagca atgattttga gtggagcaaa ttaaagcaag acatccgaga tcaattaggc
     7981 cgttacttat ttgaacaaac aaaacgccgc ccagtgatct tgccagtcat tatggaagct
     8041 actcaacgtc gaaataaaaa ataaacaaaa agagtaagat gaacctctct atagagtgct
     8101 cgtcttgctc tttttttgcg aaaaacaaat aatttttgaa tacagcatcc ctcgctttat
     8161 aataaacgat agaataaagg gagtgaatca atgatgagtc aaacaaaagt cgaacgttat
     8221 ttagaagaac aaaagatcag ctatcaacca cttgatttta gtcatgtggt agaagaatct
     8281 ttcacagaag aactacaaaa aagagggatt gatccagctt tgatatgtaa aactctcgta
     8341 gtgaaaggaa acaaaactgg ggtgatcatc gcattgattc ctttgaacga tcacttagat
     8401 tacaaaaaaa cgagacaagt gacgaaagat cgtaaagttg gttttcctgg aatggatttt
     8461 gtattaatgc atacaggata tccccacgga gcaaacaccc ctattggaat aaaactcgca
     8521 catcctgatt attgtttttt agcagacagt tctttaaaag aaaaagaaga agtaattgtg
     8581 tcaagcggtg aaatcgggag aagccttcgg ataaaagtag ccgatttaga aaagatcgtc
     8641 catcctattt acgctgattt aattagttaa gggacgagaa gagaagctaa gcagagcaga
     8701 gacctcaaac ttgagattat atctgcttgg tacttggtgc atcctgtatt atctgttaca
     8761 attgctttgt acatactgta ctatcaatcc aatgaaaata tatttagcag ctatattttt
     8821 tcattgatgt gcaagtgatg aaatcgtttt taataaaaaa gatacagaga aaacgcgagg
     8881 tgtaaaatat gaaaattacg tatcatggac attcctgttt aacaattgaa atggaaaatg
     8941 gccaacgcgt gatgattgac ccgtttatta caggaaatcc attgacagac ttaactgcag
     9001 aagaagtaaa gacagattgg ttgctgatta ctcatggtca tagtgaccac ataggagata
     9061 tgattcctat tgcaaaagaa aatcaagcca caattatcag tattgttgag attgctgatt
     9121 atgcgaacag cagaggagta gattcattcg gtatgaatat cggtggaaaa catgcgtttc
     9181 cattcggcgt ggtaaaattc gtacatgcac agcatagttc aagctatgaa gtagacggta
     9241 tcgttcagta tatgggcgag cctagcggaa ttattattca agcagaagga aaaacgattt
     9301 atcatgcagg ggatactgct tatttcagtg atctaggatt gcttgcagaa gaattcgata
     9361 ttgatgtcgc tttcttgccg attggcgata actatacgat ggggccagaa gatgcagcaa
     9421 aggctgcgca aacaattcgt gccaagaaag tagttccaat ccattacaat actttccctg
     9481 tcattgaaca agatcccgaa tcttttgtag agttactgga agatagtgaa ggaaaaataa
     9541 tgagcgtctc ggaaacgctg gaagtgtagg cagaaaatgg cgacaaaaca tgatttgatt
     9601 ttagaatata ttgaaagttt acctgttggc aatcgaattt ctgtaagaag catcgcaaaa
     9661 gaattgaatg tcagtgaagg aacagcgtac cgtgcaatca aagatgcaga aaatgtcgga
     9721 ctagtttcaa caattcaacg agtagggacc atccgtatcg aacgcaaatt aaaaaaacat
     9781 attgaacgtt tgacatttgg cgaggtcgtc aggatcattg aaggggacgt tttaggcggg
     9841 gcggcaggat tagacaaggt cttgaataaa ttcgtcatcg gcgcgatgac taaacatgcc
     9901 atggaacgct atattacgcc aggatcacta atgatcgtcg gcaaccgggc ggaagtccaa
     9961 aaattagcat tggaagatgg cgccgcagta ttgatcacag gaggattcaa tacgactgct
    10021 gagattgcaa agctagctga tgatcttgaa atgccgatct tacgcacctc ttatgataca
    10081 tttacagtcg caaccatgat caaccgagcg ttaagcgacc aattgatcaa aaaagatatc
    10141 atgcttgtca gtgatatcta tatgccagct gaaaagactc attatttgca gcagacagat
    10201 acgatttctg attatcagcg tttgtcagaa gaaacgcagc attcgcgttt tccagtcgtc
    10261 aaccgccatc atcgtttgat ggggattgtt acggcaaaag atgttttagg gaaatcgcca
    10321 aaccagatca tcgatcgggt gatgacgaaa gaaccaatca gtgtaaagaa aacaatgagt
    10381 attgcctctg ttagccatca aatgatctgg gatgggctag aagtcatgcc ggtcgtttcc
    10441 gatgacttga cattggaagg cttgattacc cggcaagatg taatgaaagc gatgcagctt
    10501 gtccagcgac aaccgcagat tgcagatacg atttcggatc agatatctgg aaatatccat
    10561 acgattgata ctgaccgtga aggaaatcgt ttggaaaacc ctaagttcaa attcgtagta
    10621 gcaccacaaa tggtcaatgg cgttggaacc atttcttttg gcgtacttag cgaaatcatt
    10681 tctgatgtcg cacaaaaaac aatggtgatg aaccaaaaac gaaatatctt gattgaacaa
    10741 gtgaacctac attatttgcg gctgatccaa ttggaaagcg aattagacat tcgtccacgt
    10801 gtattggaaa tcggcagacg ttccgctaaa ctggatatcg aagtgtttat tgaaaacacc
    10861 atcgtttcca aagcaatcgt tgtttgtcaa gtaatggaac gttcttagaa agcaggagtg
    10921 aaatgacagt acagcaagaa atagtagata caataaaaaa atacgataaa atcattatcc
    10981 acaggcattt gcgccctgat cctgatgcat taggatcaca agtaggatta gccgaactat
    11041 tacgggaaag tttcccagaa aaagaaatac gtcaagtagg tgaaacgatt gaagggcttc
    11101 gctttttagc agaaatgcaa gaagttgaag atgtcttcta ccaaggagcg ctagtcatcg
    11161 tgactgatac agctaattca cccagaatca gtgatcaaag atatcagttg gcagaaaaac
    11221 tgatcaaaat cgaccatcat cccaatgatg acccttatgg agatcttgtg tgggtaaata
    11281 caaaagcaag tagctgtagt gagatgattg cagaatttgc tttgatgttt cctgaatctt
    11341 taaagatgaa cgttaaggct gcacggttac tttatgcagg aatcgtcgga gatacagggc
    11401 gctttttgta tccctccacg acgtcccgta cattagagat tgcttcgata ttgagaaatt
    11461 atccatttga tgcaagtgct ttgaacagag agattgaaca aatgccgatg aaagtcgcta
    11521 aactttccgg ttatctttat caaaatatcc aggtggatga aaatggtgca ggaagaataa
    11581 tccttccgca agaaattctt gataaatatg gaatcgatga ttcagagaca gcttcgatcg
    11641 tttcacttcc tggagtgatt gacgaagtgc tggcttgggg gatttttgtt cagcagcctt
    11701 cagggtatta ccgcgtacgt ttacgttcta aggggccgat catcaatgaa ttggctaaac
    11761 gccatcatgg aggaggccat ccattagcaa gtggtgcgaa tgcaaaagat ttagcagaag
    11821 tagaggaaat ctataaagag atccaagata tatgccaatc gtataagagc agaaaaacag
    11881 aatgatactt cgttcgaaaa cgtgcctttt gtagtaaaaa tcacaaatgt atcttttcaa
    11941 gatcttcttt ttccgttaca atagtgctca ctaaagattt taaaggagaa agaagatgac
    12001 attaccaaat tgcccagaat gtggctcaga gtacacatat gaagatcgag gcttgttgat
    12061 ttgcccagaa tgcgggaacg aatggaacaa agaagaacaa gaaactgtca tagaagacgg
    12121 attagtggta ttagatgcaa atggaaaccg tctgttagag ggcgatagcg tgacagtgat
    12181 taaagacttg aaagtaaaag gtgcgtcagg tgccattaaa caaggaacaa aagtcaagaa
    12241 catccgttta gtagaaggcg atcataatat cgattgcaag gtagaaggat ttggaccgat
    12301 gaaactgaaa tcggaatttg tgaaaaagaa ttaaagaata ttcaaggtct tttcacattc
    12361 ttttcctact actaatgata aaataaagta aacgaaaagg agtgaaacga aaatgtatgg
    12421 ttatggaatg tatggttttt tagatccgac atttcttttg gtcattcttg gattagttat
    12481 ttccggaatt gcgtcagcgt acgtcaatag tactttccga aaatacgatc aagtgagaag
    12541 caaaaacaat gtgactggta cacaagcagc acaatatata ttacaaagtc aacagataaa
    12601 taatgtcggt gttcaacaaa ttgccggaga tctcacagat aactacaatt caggtaataa
    12661 gatgttgagt ttgtctcaag caactgcaca gtctacttca gttgctgcga taggtgtagc
    12721 agctcatgaa tgcggacatg cggtacaaga tgcttcgggt tatgtcccat tgaagattcg
    12781 tgcagcactt gttccagcag ctaactttgg ttcgatgatt tcgttcccct tgattatggt
    12841 aggtgtattg cttagctgga actctacgtt aatcaatatc gggattttcg ctttttcttt
    12901 agcattactt ttccaattgg tgacattacc agtcgaattt aacgcgtctc ggcgagcaat
    12961 ccaaattttg agtgaaggcg gactactgac ggaagaagaa gtaccgatgg caagacacgt
    13021 attatttgca gcagcactga cttatgttgc agcagcgctt tcgacattcc tgcagctgtt
    13081 gcgcttgatc ttattgttcg gcggcaaccg aagagattaa aataaagaag ttgtgaaatc
    13141 agcgctttga tctgagctaa taccgagagt tagctgattg aataccattt atttggaata
    13201 agaggatgtg acaagaattt tgtcacatcc tctttgtttt gttctcataa ccttttttca
    13261 tatacagttc atgaaccgct tttattaact tgccaaataa cactgtattg tgcgttgtat
    13321 ccagatagaa agtctccgtt tcatctgaaa ttttctcatg ctgatctgta atgatcaaat
    13381 cagcatgttc tgaatatgcg gtaattttaa cagcatcttt gcgaaataac tgctgcaaat
    13441 ggctttgaag atgataattt cctgtaaact gtttggttat ctgcaaatgg atgagtaact
    13501 gctctttcgt ttctgtttgt agtaagtgtt ggatcaattg actaaggtat tgactgattt
    13561 ggtttctttc cccatttttt gaaaaaattg tatcgatttt ttctcttaat ttcttttcct
    13621 gtataaaaga actagaatga ttggacgtgg cttctggaaa gcaaagtgta gaaaaactac
    13681 tgctggattc acccaacagt tcgaaaaaaa caactgccaa cacaagaaaa taaaaataaa
    13741 ttggtatttt attttctaaa tggttgagag gaaactgttg agttaagtaa gtcatcaatt
    13801 gaccgacttg tcgacaaata aggtttttgc ttgtggaaaa taaaaaacca atctcctgtt
    13861 tttgtcgaaa agagatttga tttggaaaaa aacaatagaa aaaataatta aagtaggctc
    13921 tctctgtttc tgtctcttca caagggcgac tcactgaaaa agaagtaaag tattctgaat
    13981 aatcatgtgt atgctgcaat aagtccatca attcagtgat ttcagaagaa gtttttggaa
    14041 aatatgcctg ttgtttgttt cgtaatatga ataaagttaa cagcaagtag atattttttt
    14101 cttgttttga aattttttct atttgctcat ctgtactgtt catttttagt gcatttaatt
    14161 caagtgagga aaatggccaa tcgttacctc caaatgtatc tgaaagaaaa aagtaaagat
    14221 agagacggat agataactca tttccttcaa gagtacagca attttttaca gataggatcc
    14281 gtaaatggaa tttttttaag gaatgattga gctgttttgt cagtttttcg agataggagg
    14341 gcgatatata aagttgttca agtaaatatt tttttggtac agcagtttgt ggataattga
    14401 ttagtaacat caaatagtta aaacgattgg attcttttag gtagcataat tttaacttag
    14461 aaaaaacagc taacttttta gtttgatcgg tttctttggt ttggatctct cctttatgat
    14521 tagaataaat aaattcatca atcaataaaa attgcttgag tgatacattt atttttcgga
    14581 tcagttcttt gattgtccgt tttggaatat gtaagtcatt ggataattct tgtaaagaac
    14641 aattattttt ttgaatgacc atttgtagaa caagtaactc tttttttctt gcttcaatta
    14701 gcataaatac ctccttttta ttctgttttt gtatatagtt agacaaaaac tgtgagtgga
    14761 aagtcatttc agtctgt
//
LOCUS       DAKBVG010000053        14658 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000053 DAKBVG010000000
VERSION     DAKBVG010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14658
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.053"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            3..815
                     /locus_tag="OUD09_001150"
     CDS             3..815
                     /locus_tag="OUD09_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HCT9692431.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            812..1612
                     /locus_tag="OUD09_001151"
     CDS             812..1612
                     /locus_tag="OUD09_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCT9692432.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1773..3041
                     /gene="rseP"
                     /locus_tag="OUD09_001152"
     CDS             1773..3041
                     /gene="rseP"
                     /locus_tag="OUD09_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HCT9692433.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3109..4818
                     /locus_tag="OUD09_001153"
     CDS             3109..4818
                     /locus_tag="OUD09_001153"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCT9692434.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5030..9382
                     /locus_tag="OUD09_001154"
     CDS             5030..9382
                     /locus_tag="OUD09_001154"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HCT9692435.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9526..9999
                     /gene="rimP"
                     /locus_tag="OUD09_001155"
     CDS             9526..9999
                     /gene="rimP"
                     /locus_tag="OUD09_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCT9692436.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10023..11198
                     /gene="nusA"
                     /locus_tag="OUD09_001156"
     CDS             10023..11198
                     /gene="nusA"
                     /locus_tag="OUD09_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCT9692437.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNSKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11220..11513
                     /locus_tag="OUD09_001157"
     CDS             11220..11513
                     /locus_tag="OUD09_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HCT9692438.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11510..11821
                     /locus_tag="OUD09_001158"
     CDS             11510..11821
                     /locus_tag="OUD09_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HCT9692439.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11834..14140
                     /gene="infB"
                     /locus_tag="OUD09_001159"
     CDS             11834..14140
                     /gene="infB"
                     /locus_tag="OUD09_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCT9692440.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14167..14514
                     /gene="rbfA"
                     /locus_tag="OUD09_001160"
     CDS             14167..14514
                     /gene="rbfA"
                     /locus_tag="OUD09_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCT9692441.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
BASE COUNT         4999 a         2589 c         3267 g         3803 t
ORIGIN      
        1 gaatgttacg tttttttccg caaaaaaaca aatatattca agaaaaaagc gaatttcaat
       61 ttaataccga tggaccaatt cctcagcaca ttgccatcat catggacggc aatggtcgtt
      121 gggcgcaaaa ccgtcgtttg ccacgagttg ctggccataa agaaggcatg gaaacagtaa
      181 agaaagtcac aaaaaaagca tcaaggcttg gagtgaaagt gttgacgctt tatgcctttt
      241 cgactgaaaa ctggaagcga ccaaaagatg aagtaagttt tctcatgcaa ctacctgtcg
      301 acttctttga tacgttcgta ccagaattga ttaaagaaaa tgtcaaagtt catgtcatgg
      361 gttatgaaaa cgtattgcca gaacatacgc aagatgctgt aagacgtgca attgagcaaa
      421 caaaaaacaa tactggaatg gtattgaatt ttgctttgaa ttatggaagt agagcagaga
      481 tcgtgactgc tgtccaagaa attgccgaag aagtagcaaa aggagaaata cacgcagaag
      541 aaatcgatga tgaattgatt gctaagcatt taatgacagg tttcttgcct aaagagttac
      601 aagatcctga gctgatgatc cgtacaagtg gagaagagcg aattagtaat tttctgttat
      661 ggcagatcgc atatagtgag ctgtatttta caaaagcgct ttggcctgat tttgatggtg
      721 cgcatcttga agaagcaatc gcctcttatc aaaatcgaga ccgtcgattc ggcggtgtaa
      781 aaaaaacaga agatgagaaa ggggatcagt catgaaacaa cgtgtgatca cagcagctgt
      841 ggcgctagtc ttgtttattc cgattatatg gatcggaggc atagcggttg agcttacagc
      901 agcaatctta gcggtagtag gagtttacga gctgttccgg atgaaaggat tgacactctt
      961 aagttttgaa ggagttttat ccgccgttgg tgcagtgtta cttgttttgc caaaagaaag
     1021 atggtttttc tttcttccag agaatgtaag tacatttatg ttattttatc ttatagttat
     1081 gattctttta ggaacatcag ttatttctaa aaatacgtat acgattgatg aagcaggttt
     1141 tcctgtgctt gtcagcttgt atgtgggaat aggtttccag aattttgtca atgcacgaac
     1201 agaaagcttg gctgttttga tatttggtct atttattgtt tgggcaactg atatcggagc
     1261 atatatggtt ggtcgacgtt atggtcagca taagttaagt cctgatattt cgcccaacaa
     1321 aacgattgaa ggtgcgctcg gtggtatcgg tagtgctgtt gtagtcgcat ttctttattt
     1381 attgctatac ccagcaaaag aactattcaa tcatggtatg tttgtcatgc ttctgatgac
     1441 gattattttt tcagcagtcg gacaatttgg tgatttggtc gaatctgcaa tcaaacgtca
     1501 ttatgatgtg aaagactcgg gaaatatctt accaggccat ggtggaatct tagaccgttt
     1561 cgacagtttg ctgtttgtat ttcctatcat gcatttattt ggcattattt agtaagtata
     1621 cctgcgtttc tttgtgaatt tcataaaaga cgcaggtttt tatttttcat tatattgata
     1681 ctattggtgt ctttggtata ctatggtaaa attatagtgt tttatcgttg ttttataaaa
     1741 aacacaaaaa acaagtaaag gagaaaaaag ctatgaaaac gattctgaca tttatcatcg
     1801 tttttggtat attagtgatt gttcatgagt ttggtcattt cttctttgcg aaacgatcag
     1861 gaatcctcgt ccgcgaattt gctatcggta tgggtccgaa aatttatggg catcaagcaa
     1921 aagacgggac aacatatacg cttcgcctat tacctattgg cggttatgtt cgaatggcag
     1981 gcaatgggga tgatgaaaca gaaatggcac caggaatgcc tctttctctt ttactaaatt
     2041 ctgacggcat cgtagagaag ataaatttaa gtaagaaaat ccaattgaca aatgctatcc
     2101 cgatggaatt gagccgttat gatttagaag atgagctgac tatcactggt tatgtgaatg
     2161 gagacgaaac agaagtagta acctaccctg ttgatcacga tgcgacgatt attgaaaacg
     2221 acggaacaga aatcagaatt gcgccaaaag acgtacagtt ccaatcggct aaattgtggc
     2281 agcgtatgct gaccaacttt gctggaccca tgaataactt tattttggca atcgtgttat
     2341 tcatcattct ggcatttatg caaggtggtg ttcaagtgac gaacactaat cgcgtaggag
     2401 agatcatgcc taatggagct gcggcagaag caggcttaaa agaaaatgat gaggtagtca
     2461 gtgtggacgg aaaagaaatc cattcatgga atgatctgac aacagtcatt acgaaaaatc
     2521 caggtaaaac attagacttt aagatcgaac gagaaggtca ggtgcaatct gtcgacgtga
     2581 caccgaaatc tgtagagtca aatggcgaaa aagttggcca acttggtata aaagccccaa
     2641 tgaatacagg ttttatggat aagatcattg gagggacgcg tcaagctttc agcggttcat
     2701 tggaaatttt caaagcctta ggatcactat ttacaggttt cagtttagat aaattaggcg
     2761 gacctgtcat gatgtaccaa ttgtcttcag aagcagcaaa tcaaggtgta acaactgtta
     2821 ttagtttgat ggcgcttctt tcaatgaatc tcggaatcgt caatctgctt ccgattcctg
     2881 ccttagatgg cgggaaatta gtcttgaata tttttgaagg gattcgcggg aaaccattaa
     2941 gtcaagaaaa agaaggaata ttgacattag ctggctttgg atttttaatg ttgttgatgg
     3001 tgctagttac atggaacgat attcaacgat tctttttcta gaaaaagttg acaaataggg
     3061 tataataaca aacgataact attttgagga aataaaggag tatatagaat gagacaatca
     3121 aaaatgttga ttccaacatt acgcgaagtt ccaaatgatg cagaagtatt aagccatcaa
     3181 atcttgctaa gagctggata tatccgccaa gtatcagcgg gtatttattc atatctgcca
     3241 ttagcaaatc gtgtattgga aaaactgaaa cgcattatgc gggaagaatt tgaaaaaatc
     3301 ggagcggtag aaatgctgat gcctgccatc ttgccagcag aactttggga agaatccggg
     3361 cgttacgaaa catatggacc aaacttgtat cgtttcaaag accgcaatga tcgaaaaatg
     3421 atccttggac caactcacga agaaactttt actgatttga tgcgtaatga aatcaattca
     3481 tataagaaat tacctttaaa tctttatcaa atccaaccta aatatcgtga cgaaaaacga
     3541 cctcgcttcg gtttgcttcg cggaagagag tttatcatga aagatgcata ttctttccat
     3601 gcaacggaag aaagtttgga cgagacatat aaagattacg aaaaagccta taccgaagta
     3661 ttcaagcgtt gtggcttaga atttcgctcg atcatcggag acggcggcgc aatgggcggg
     3721 aaagactcaa aggaattcat ggcgatctca gaaatcgggg aggatacgat ctgttattcg
     3781 actgaaagtg attatgctgc aaacttggag atggctacaa gctactatgt atcaaaaaaa
     3841 tcgcatgaaa cacagcttga attggagaaa gtagctacac ctaacgtaaa atcaattgac
     3901 gaagtagcag atttcttttc tgttgaacct caaaaaatca tcaagtctgt attgttcatt
     3961 gcggatgaac aacctgtcct tgttttggta cgtggagacc atgaagtcaa tgacgtaaaa
     4021 ctgaaaaact ttttatctgc tgacttttta gaagaagcaa cagaagaaga agcacaaaaa
     4081 tatctaggtg ctgagtttgg ttctgtcgga ccagtcggtg tttcagatga cgtaaaagta
     4141 tatgcagacc gttatgtcca agatttagcg aatgccatta ctggagcgaa cgaaacagga
     4201 ttccactata aaaatgtgaa tccagaacgc gatttcactg tgctttctta tgaagatctt
     4261 cgttttgtac aagaaggaga tccttcacca gacaacaacg gtgttcttgc ctttactcga
     4321 ggaatcgaaa tcggacatat attcaaaatc ggcactcgtt acagtgaatc tatgggagca
     4381 actgtactag acgaaaatgg acgagaaaaa tttgtgatta tgggctgtta tggtatcggt
     4441 gtaagtcgtt tattatcagc aatcgtagaa caacattcag atgaacaagg aatcaattgg
     4501 ccaaaaggaa ttgcgccatt tgatctgcat atcgtccaaa tgaatctaaa agacgaatac
     4561 caaacaagcc tgacgaatga actagaagaa gcgatgacaa aagctggcta tgaagtactg
     4621 gttgatgaca gaaatgaacg cgcaggagtg aaattcgcgg attctgattt gattggctgt
     4681 ccaatcagaa tcactgtcgg taaaaaagca gtggatggaa tcgtagaagt gaaaatcaag
     4741 aaaactggcg aaatggttga agtacgtaaa gaagaattgg cgaatacttt accaatcctc
     4801 ttgaaccaag gcgaataata agcgttaaaa cacagaaaat aaaaatagct gaatcagctg
     4861 tttttgacaa tctgatgatt cgcttttaaa attggaactg cgatttgaat cgcagttcca
     4921 attttttaat gtaaaactca gaaaaaacga gtatactaaa aggtactgaa aaagaagagg
     4981 ataagatgcg atcaattgca ttgtttctca tcagaaaggg cgtatatctt tgtcagaaaa
     5041 acgagattta tttgaaaaac tgttggaaca aatccaacta gaggaagcag aaaaaaatca
     5101 tccactgctt actgctggtg aaatggatag tgtagtggtc catcgtaaat cgcgtttatg
     5161 ggaatttacg ctacattttt ccaaaatttt gcctattatg ctatatcggt cattgactca
     5221 gcatctgact atcgctttta aagatattgc acaagtcaaa ctgaatatct tagcagatga
     5281 ccagacattc gatgaacagc tcttgcaaga ttactgggcc caagctttgg aaaatcagca
     5341 atgcgataca ccactagcac agcaagtatt gaaaacgcag gtacctgtga taaaagatcg
     5401 taaagtcatc ttgccaatcg acagtacagg agctatttct tatttgaagc aacaatactt
     5461 gccattagta gaagagttgt tcgtcagtta cggattccca aaatttcgta tcgaaccaga
     5521 agtggatgag cagcaagcgg aacgtgtatt gaaacttttt gaagaacgca aacaagaaca
     5581 ggcagaagcc tttatgaagc aagcagctga aagtctagtt gtccacgaac aaaagaaaaa
     5641 ggaaagaaaa gagcaatcgc cagctcttga aggacccatc cagctaggac gaaatattcc
     5701 agccgatgaa ccaacgacac caatgatcag tatcgttgaa gaagaacgaa gagtcacact
     5761 tgaggggtac gtattcgaca aagaagtcag agaactacgt tcaaaacgca agatcttgac
     5821 tttgaaaatc acagattata cttcatcatt tatcgtcaaa aaattctcga acggcgaaaa
     5881 agatgaacaa gtatttgatg cgatcagtgt cggtagctgg ttaaaggtac gaggaagtat
     5941 tcaagaagat acgtttgtcc gtgatttggt catgaatgcg caagatatca tagaagtcaa
     6001 acatacacct cgcaaagatt atgctcctga aggggaaaaa cgagtagagc ttcatgttca
     6061 tagcaatatg agtacgatgg atgcaacaaa cagcatctct gatcttgtgg cacaagcagg
     6121 aaaatgggga caccgagcta tcgccattac agaccatggt ggtgcgcaag cctttccgga
     6181 agcacatagt gctggaaaaa aagcaggtgt gaaaatctta tacggcgtgg aagcgaatgt
     6241 cgtagatgat ggtgtcccaa ttgcttataa tgatgcacat gaagcattga gtgaagccac
     6301 atacgtcgta tttgacgtgg aaacgacagg attgtctgca gtatatgaca cgatcatcga
     6361 gctagccgca gtcaaaatgt ataaaggaaa tgtgatcgaa agctttgatg aatttatcga
     6421 tccaggacat ccattgtcac gaacaacggt agatctgact ggtatcacgg atgaaatggt
     6481 tcgagggtca aagtcggaag aagaagtatt gcggatgttc cttgaatttt caaaagatac
     6541 aattttggta gcccacaacg cggcttttga tatggggttc ttgaatacta gctacgcaag
     6601 gtatggtatt cctgaagcgg ccaatccagt cattgatacg ttggaattag ctagatatct
     6661 gtatcctcaa ttcaaacgtt tcggattagg tgtgttatct aaaaagtttg gtgttagctt
     6721 ggaacagcat caccgagcaa tctatgatgc tgaagcaact ggtcacttag catggatctt
     6781 tgtgaaagag gcaatggaca atcataacat gctttatcat gatcagctaa atgagcatat
     6841 cggagaagga gattcttaca aaagggctcg acctttccat gtaacgattt tagcaaaaaa
     6901 tcaggcagga ctgaaagatt tgttcaaact gatttcaatg tcgaatgtcg aatacttcga
     6961 acgcgtccca cgaataccac gctcccaatt gaaaaagatg cgtgaaaatc tattgatcgg
     7021 ctcagcttgt gataagggag aaatattcga agccatgatg caaaaagggg tagaagaggc
     7081 aagaaaccga gcaaaattct atgattatat tgaagttatg ccaaaagcgg tctatgcgcc
     7141 tttgatcgag caagaattgg taaaaaatga acatgatttg gaagaaatta tccaaaattt
     7201 ggtagaaatc ggaaaaagtt tggataaaat cgttgttgca acaggtaatg tacattatct
     7261 gaacgaagaa gatgccattt accgaaaaat tttgatcaat tctatgggcg gagcgaatcc
     7321 gttgaatcgt catagtttgc cggatgtcca tttccgaaca accgatgaga tgctaactgc
     7381 atttcacttt ttaggggaag aaacagcgaa agagatcgta gtagaaaata cgaataaaat
     7441 cgcagatatc tgcgaagaag tgattccagt caaagatgaa ctttatacac cgaagattcc
     7501 aggttcagaa gatgaaatat cagaattgag ttacacaaaa gcaaaacaaa tgtacggaga
     7561 tccgttacct gagatcattc aaaaacgatt agaaaaagag ttgaattcta ttaatggtaa
     7621 tggcttctcg gttatttatc tgatcgccca aaaactagtg cataaaagta atgaagatgg
     7681 ctatctagta ggttcacgtg gctctgttgg ctctagcttt gttgcgacga tgacaggaat
     7741 cactgaagtt aatccattag cccctcatta ttattgtcca gagtgccaat attccgagtt
     7801 ttttgaagat ggtacgtatg gttctggatt tgacatgcca gaaaaacaat gtccaaaatg
     7861 tggtgcacgc ttaaacaaag acggtcatga tattccattt gagacatttt taggattcca
     7921 tggagacaaa gtgcccgata ttgatttgaa cttctcaggt gattatcaag cagaagccca
     7981 taactatacg aaagtattgt ttggcgaaga ctatgtttac cgtgccggaa cgattggtac
     8041 agtcgccgac aaaacagcat atggctatgt aaaaggatac gaaagggaca ataatctgca
     8101 gtttcgaagt gcagaagtcg acagattagc aaaaggcgct actggagtca aacggacaac
     8161 cggccagcat ccagggggga tcatcgttat tcccgattat atggatgtct atgattttac
     8221 gccgatccaa tatccagcag acgatcaaaa ttcagaatgg aaaacgactc attttgattt
     8281 ccactcgatt cacgataatg tattgaaatt agatattcta gggcatgatg acccaactgt
     8341 tattcggatg cttcaagatt tatcagggat cgacccacaa acgatcccta cggatgaccc
     8401 ggaagtgatg cggatatttg ccggtcctga agtacttgga gtaagtcagg aacaaattta
     8461 ttccaaaaca gggacattag gtattccaga attcggcact cgttttgttc gagggatgtt
     8521 agaagaaacg catcctacaa cttttgcaga actactgcaa atttccggac tatctcatgg
     8581 tacagacgta tggttaggaa atgcggaaga attgattcga cgaggagatg cgacactggc
     8641 ggaagtaatc ggctgtcgtg atgatattat ggtttatttg atccatgctg gattagatag
     8701 cgggatggca tttaaaatca tggaaactgt tcgtaaagga caatggaaca agatccctga
     8761 tgaattgcga gaaacctatc tatcggcgat gaaagaaaat aatgtgcccg actggtatat
     8821 cgattcttgt tcaaaaatca aatatatgtt tccgaaagcc catgctgcag cttacgtatt
     8881 gatggctctt cgtgtcgctt atttcaaggt ttatttccct attttgtatt actgtgcata
     8941 cttttctgta cgtgcagacg actttgatct agtctccatg tgcaaaggaa aagatgctgt
     9001 gaaacaagca atgaaagaaa tcacggacaa gggattagat gcttccgtaa aagaaaagaa
     9061 tcaactgaca gtgctggagt tagccaatga aatgctggaa cgaggattca aatttggaat
     9121 gattgatttg tataaatcgg atgccgttaa ctttgttatt gaaggtgata cgttgatcgc
     9181 accatttaga gctgtcccaa gtttgggaac caacgtggcg aaacaaatcg ttgaagcaag
     9241 aaaagacggt ccatttttat ccaaagaaga tcttgccacc cgcggtaaag tttcaaaaac
     9301 attgatcgaa tatatgaacg acaatggcgt attgaaagat ctaccagatg aaaatcaatt
     9361 atcgttattc gatatgctat aagcatctgt tttcctgtct gcttgtaatt ttaagcagag
     9421 tatggtatag ttcttatagg taatgatact aactgtgagt gagcggattt ttcgctcact
     9481 ctttttaatg gaattacgca cttaattttt aaggaggcga aacttttgag tagcgtcgtt
     9541 gaaacagtca cagaaatggt gacaccgatt ttagatgaac aaaattttga acttgtcgaa
     9601 gtcgaatttg taaaagaagg aaaaagctgg ttcttacgag tatttattga caaagaaggc
     9661 ggtatcgata tcgaagaatg cgcctttgtc agtgagaaac tgagtgaaaa acttgatgca
     9721 gcagatccag atccgattcc tcaagcttat ttcctggagg tatcctcccc gggagcagag
     9781 cggccattga aaaaagaaag cgactatgaa aaggcacttg gtgattatgt ccatgtctcc
     9841 ttataccaac cagtagatgg cgaaaaacaa tatgaaggat ttcttcaatc atttgatgct
     9901 gaacagctta cattgaaaat acgcataaaa acacgagaaa aagaattgac ttttgaccga
     9961 aaaaatattg caaaagctcg cttagctatc caattttaat cacggagagg aatcagaaaa
    10021 aaatgagtaa agaaatgtta aatgcgttag acgctttaga agctgaaaaa gggatctcaa
    10081 aagaaatcgt gatcgatgca ttagaagcag cattggtttc tgcatacaaa cgtcactatg
    10141 gccaagcaca aaacgtcgaa gtggaatttg atcagaaaaa aggaaagatc catgtctatg
    10201 cggtaaaaga agtaacggaa gaagtgatgg attcacagtt ggaagtctca ttgaaagatg
    10261 cacttttaat caatccagca tatgagatcg gtgatacgat ccgttttgaa gtaacaccaa
    10321 aagattttgg acggatcgct gcgcaaacgg ctaaacaagt tattttacag cgtgtacgtg
    10381 aagcagaaag aacgattatt tataacgaat tcagtgctta tgaaaaagac atcatgcaag
    10441 gaatcgtcga acgccaggat aaacgttata tttatgtcaa tcttggtaaa attgaggcag
    10501 ttctttctaa acaagatcaa atgccaaatg aattttatca accacatgat cgaatcaaag
    10561 tttatgtttc ccgagtagaa aatacgtcta aaggtccgca agtatttgtt agccgcagcc
    10621 atccagacct attgcgtcgc ttatttgaac aagaagtacc agaagtctat gatggaatcg
    10681 ttgaaattgt cagcatcgcc cgtgaagctg gcgatcgttc aaaagtggca gttcgctcaa
    10741 atgatccaaa tatcgatgca gtcggtacat gtgtcgggcc aaagggacaa cgagttcaag
    10801 caatcgtcaa tgaattaaaa ggcgaaaaca tggatatcgt cgaatggaat gaagatccag
    10861 ctgtattcat cgcaaatgca ttgaatcctg cacaagtcat ggatgtgatc ttcgatgaga
    10921 caaattcaaa ggcctgcaca gttgtcgttc cagactacca attatcgcta gcaatcggta
    10981 aacgcggaca aaatgcacgt ttagctgcta aattgactaa ccataaaatc gatatcaaat
    11041 cagaatctga catgacggaa ttttatgaaa atcaagaaca acaaaaagaa acagaagaac
    11101 tgcatgatga agcaatcgtt cagtcagatt taacagacga tgaatatgaa acaattgcat
    11161 tcaacaatga aacggtcgaa gaaaaaccag aagcttaact ttagtaagga ggcaaagaga
    11221 tgaaacaaag gaaaattcct ttacgcaaat ccgtggtttc aggtgaaatg aaacctaaaa
    11281 aagagatggt ccgaatcacc cgttcaaaag aaggggaagt atcgatcgat ccaactggaa
    11341 aaatgcctgg acgaggagcg tatgtctcac ttgagccgga agaagtacaa caagcttggg
    11401 ataaaaaaat tctcgatcga gtactcgaaa caacattaac agatgagttt tatcaagaac
    11461 ttcttgacta tgtcactcac caaaaagcac gtaaagagtt atttggaaaa tgaatacagt
    11521 taatcggcaa aaagccatga atctcatcgg tctagcgatg cgcgcgggaa aactaatcac
    11581 gggtgaagaa ctgaccattg ctgaaatccg caaacaaaaa gcaaaattgg tgtttgtggc
    11641 aaccgatgca agtgagaata caagaaaaaa gataaaagat aagagttcat attatgaagt
    11701 tccttgcttt gagctatttt ctgaagcaga aatcacgcag atgatcggaa aacctcgcaa
    11761 ggtattcgga ataatggatg ccggatttgc gaagaagacc aaggagctaa ttgaaggtta
    11821 ggaaggtgat tgcatgggca acaaaagaat ctatgaatta gcaaaagaac tcaaaaaatc
    11881 tagtaaagac gtcgtggaaa aagcacaaca acttggcatc gatgtgaaaa accatatggg
    11941 tgcgattact agcagtgatg agaagaaatt gcaacaagcg ttcaaaaacc caagcacgaa
    12001 acaacaagct gcacaacaag caagcagaaa accagccaat caacagccga acgatcaacg
    12061 taaaaaagaa acaacgaaca atcaacaaaa aaataaaaat aaccgcagtt atcaagatcg
    12121 cggtcaaggg agcggtcaag tgaatcaagg aaaaaaacca acaacaaacc aaaataatac
    12181 gggaaatacc agcagccaaa atcgtcaagg aacgagccaa ggcggcaatc aaaaccgtac
    12241 taacaataac aacaaaccaa acaataacaa tcgcggcaaa tttaataata ataaccgtaa
    12301 tcgttataat aaaaaaggta aaaaaggcaa acagcaacaa tcaactaaac cagctgtacc
    12361 accacgcaaa ttccgtgaat tgccagatgt attagagtat actgaaggta tgaacgtagc
    12421 agatattgcg aaaaaaattc atcgtgagcc agcagaaatc attaaaaaat tatttatgct
    12481 tggtgtcatg gtcaatcaaa accaagcact ggataaagat acgattgaac tgttagcgac
    12541 ggactatggc atggaaccac aagaaaaagt ccaagttgac attgctgata tcgataaatt
    12601 cttcgaggca gaagaagtcg atccagataa attagtctca cgtccgccag tagtaacgat
    12661 tatgggacac gtagaccacg ggaaaacaac cttattggat acattgcgcc attcacgtgt
    12721 aacaagcggc gaagccggcg gaatcacaca gcatatcggt gcctaccaga tcgatattga
    12781 tggtaaacca atcactttct tggatacacc aggacatgcg gcctttacaa gtatgcgtgc
    12841 acgtggagca agtatcacag atatcacgat cttagttgtt gctgcagatg atggcgtcat
    12901 gccacaaaca gttgaagcaa tcaaccatgc caaagctgca ggcgttccaa tcatcgtcgc
    12961 tgtaaataaa attgataaac caggtgcaaa tcctcagcat gttatgcaag aattaagtga
    13021 gtatgaatta attcctgaag catggggtgg agaaacgatt tttgttgaga tctcagcgaa
    13081 attcggccaa aacatcgaag aattgctgga aatgatcctg ttagtcgcag aagtagaaga
    13141 tttaaaagca gatccaacac aacgcgcaat cggaacagtg atcgaagcac gtcttgataa
    13201 aggtaaaggt cctgtgtcaa ccttacttgt ccaagaagga acacttcacg ttggtgaccc
    13261 aatcgttgtc ggtaatactt acggacgtgt ccgtgtaatg acaaacgatc ttggccgtcg
    13321 cgataaagaa gcaggacctg caacgccagt agaaatcact ggcttgaatg atgtacctca
    13381 agcaggtgac cgtttcgtag tcttcgaaga cgaaaagaca gcacgtgcag cgggtgaaga
    13441 acgcgcaaaa cgtgcattat tggaaaaccg tgctgccaca agccgtgtga ctttggacaa
    13501 cttatttgaa agcctgaaag aaggcgaatt gaaagaagtt aacgtaatca tcaaagcaga
    13561 tgtacaaggt tcagcagaag cactggctgc atcattgaag aagattgatg tggaaggtgt
    13621 ccgtgtaaaa atcgtccatt cagctgtcgg agcaatcaat gaaagtgatg taaccttagc
    13681 agcagccagc aacgctatta tcatcggttt caacgttcgt cctacaccgc aggcaaaagt
    13741 gcaagctgat actgaagaag tcgatattcg tcttcaccgt atcatctata aagccatcga
    13801 agaaatcgaa actgcgatga aagggatgct agaccctgaa tttgaagaaa aaatcaccgg
    13861 tcaaatgatc gttcgtgaaa ccttcaaagt ttctaaagtt ggtacgattg caggtgccta
    13921 tgtaacagaa ggatatattc gtagagatag cggtgtccgt gtaatccgtg acggaatcgt
    13981 aatctatgaa ggccaacttg caagtctgaa acgattcaaa gatgacgtga aagaagtcaa
    14041 aatgggttat gaatgtggtg cgatgattga aaaattcaat gacatcaaag tagatgacgt
    14101 aattgaagga tttatcatgg aagaagtcaa aaatgactaa aaactgataa aaggaggcca
    14161 atcattatgg ctaactaccg tgatcgccga gtcggtcaag aaatcttaaa agaagtcaat
    14221 gatattttgc gcaaaaaggt ccgtgaccct cgtgtggaga acgtaacgat cacagatgtg
    14281 aaagttacgg gggacttaca gcaagcaacg atctattaca gcattttatc tgacttagca
    14341 tcagataaga aaaaagcaca agatggattg aataaagcat caggattgat tcgtcgagag
    14401 ttagggcaca atctaagtat ttataagaca cctgaattgt tctttgaact tgatgaatca
    14461 gttgtctatg ggaaccatat cgatgaattg cttcgcgact tgaacaaaga ctaatcaaaa
    14521 aatcccgcaa atcctgtgaa atcaggggtt gcgggatttt tttgttttca ttaagacgta
    14581 ttacctaaga gttccatgac tttagtctgg tagcgtgtca gctaaccaaa aaagagaaaa
    14641 agaaaagtaa aaaattcg
//
LOCUS       DAKBVG010000054        14531 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000054 DAKBVG010000000
VERSION     DAKBVG010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.054"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(63..257)
                     /gene="ssrS"
                     /locus_tag="OUD09_001161"
     ncRNA           complement(63..257)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="OUD09_001161"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(329..1615)
                     /locus_tag="OUD09_001162"
     CDS             complement(329..1615)
                     /locus_tag="OUD09_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HCT9692442.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            1753..2424
                     /locus_tag="OUD09_001163"
     CDS             1753..2424
                     /locus_tag="OUD09_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HCT9692443.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            2390..2878
                     /locus_tag="OUD09_001164"
     CDS             2390..2878
                     /locus_tag="OUD09_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HCT9692444.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            2891..3838
                     /gene="prmA"
                     /locus_tag="OUD09_001165"
     CDS             2891..3838
                     /gene="prmA"
                     /locus_tag="OUD09_001165"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCT9692445.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            3839..4603
                     /locus_tag="OUD09_001166"
     CDS             3839..4603
                     /locus_tag="OUD09_001166"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCT9692446.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            4836..7049
                     /locus_tag="OUD09_001167"
     CDS             4836..7049
                     /locus_tag="OUD09_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HCT9692447.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            7060..7506
                     /locus_tag="OUD09_001168"
     CDS             7060..7506
                     /locus_tag="OUD09_001168"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCT9692448.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            7677..8936
                     /locus_tag="OUD09_001169"
     CDS             7677..8936
                     /locus_tag="OUD09_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HCT9692449.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(9025..9972)
                     /locus_tag="OUD09_001170"
     CDS             complement(9025..9972)
                     /locus_tag="OUD09_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCT9692450.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            10366..11658
                     /locus_tag="OUD09_001171"
     CDS             10366..11658
                     /locus_tag="OUD09_001171"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCT9692451.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            11673..13442
                     /gene="aspS"
                     /locus_tag="OUD09_001172"
     CDS             11673..13442
                     /gene="aspS"
                     /locus_tag="OUD09_001172"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCT9692452.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            13551..14426
                     /locus_tag="OUD09_001173"
     CDS             13551..14426
                     /locus_tag="OUD09_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCT9692453.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
BASE COUNT         4757 a         2625 c         3163 g         3986 t
ORIGIN      
        1 aataagcaac aaatagaaaa agcaatttgc caagagcaaa ttgctttatt ctttaaaaga
       61 tggtaatctc cgttaatgcc gttgtttgtc ctgtagtatt gatcccgcga attcaagcag
      121 gtgggtccca tggtcgtagc ttcgaactac caaatgataa ggcatgttac cagcagtgac
      181 acaagacggg tcccaagata tcaataaaaa tgttcggtca acacacaaag aggacaattc
      241 gaacatcaca gatttcccac ctctatagta gcatatgaat ttaaaaagag caatgagaga
      301 gctcgcagaa acagaaccgt tttcttgtct agttttttgg aggatgcttt ttccactctt
      361 ttatacggta atactgttga cccagagcct gttcatattt ccctgtatct tttggctgat
      421 aatactgagc attcttcagt ttatctggta aatattgctg attgacccaa gcctgatcaa
      481 aatgatgagg atactgataa cccacgccac ggttgagtga tttcgcacct ttgtaatggc
      541 tatctctcaa atgatcagga acatctccag ctttgccctc tcgaatatca gcaagtgctg
      601 catctaatgc catgtaggca gaattggatt ttggcgacaa gcaaagatcg acgacgacgt
      661 ctgctagtgg gatacgtgcc tcaggcaatc ccaatttttc agcagctaat acagcgttca
      721 ctgtacgagc cgcagcagca ggattaccta aacctatatc ttcataaccg ataaccatca
      781 gacgccggca aatacttgcc agatcacctg cctcaactaa acgggcgaga taatgaagtg
      841 cagcatccac atcacttcca cgaatcgatt tttgaaaagc cgaaataacg tcataatgag
      901 catctccatt tttgtcatgg gtaagagctt ttctttggac acattcttca atgatcgaca
      961 gtgtaagatg aatccttcct tctttatctg aaggagtcga tagtgtagcc agttccagcc
     1021 cattcaatgc actccgcaaa tcgccgttcg ttgcacgcga aaggtgaaga agagcatctt
     1081 cgtctaactg gattgcttgt tgtccaaggc cgcgttcttt atcccttaaa gcatgttcga
     1141 ctgccaactg gatgtcttgt tctgttaatg gtttgacttc aaagatctgt gtacggctgc
     1201 ggatcgctgg attgatggta atataaggat tctcagtagt cgctccgatc aagatgattc
     1261 ggccactttc taaatgagga agcagaaaat cctgttttgt tttatctaat cggtgtactt
     1321 catccaataa gaggatcact gtaccactca tttttgcttc ttccgctaca acttgtagat
     1381 ccttttttga atctgtagcg gcgtttagca tgcgaaatgc ataattagta gagccggcga
     1441 tcgcacttgc gatactcgtt tttccagtac caggaggtcc atatagaatc atggaagaga
     1501 gcatgcgagc atcgaccatg cggcgtatga tttttccttc cccgactaga tgctgctgac
     1561 caacgacttc atcgatcgtt cgaggtctca tgcgataagc caatggctgt tgcataggat
     1621 tttcctcctt cttgttttta cgtcccttaa ttatatcata tttagcaaga tgcctagaaa
     1681 gaacagcctt ctgttgtttt gttttcatag cttatccaac taatattgtg tatgataaag
     1741 taggtgagaa aaatgacgga taaacaaaag atagatgaat ttttttggaa taaaacgacg
     1801 ccagaagtgg cacgggattt attggggatg tatttggagc atgacacacc agaaggaaga
     1861 ttagctggat atattgtaga cgcggaagca tacctaggtc cagaagacga ggccgcccat
     1921 agctatggtt taagaagaac accgcgtgtg cgggcaatgt atgaaaaacc aggtacgatc
     1981 tacctttaca cgatgcatac tcatcggatt ttgaacatca ttactcagcc agaagggatt
     2041 cctcaaggag taatgatacg tgcaatcgaa cccgccgcaa tgattgatca aatgagtaaa
     2101 aatcgtggcg gaaaaactgg tccagatatc agcaacggtc cgggaaaatt agtggaagca
     2161 ttagcaattc ctcaagaact ttatggtcag tctatagcag atagttcgtt acgcttagtt
     2221 tttgagaaaa aaaagacacc aaaaaagatc aatgctttgc ctagaatcgg tatcccaaac
     2281 aaaggtgtat ggacagaaaa gccgttgcgt tttgttgtgt cgggaaatcc ttatatctct
     2341 ttacaaagaa aaaaccaaat cgagaaagat tggggttgga ggaaagaaaa tgaaaaagaa
     2401 ggatctatta acatatttag atgaaataat tgaaaaacaa gtgacagact atgatgtagc
     2461 tttagattgg gatacaaaaa atcatacgat cgagatcgtt gttcgcttgt ttgcagaaaa
     2521 taaagcacaa tttgagattg atgatgcaga agggatcgtt tcgcaagaac caattatcga
     2581 atttgaagac ggcgtacttc tttttaatcc tcagaaatct gtttttgatg aacgcaatta
     2641 tctagcagta atcccttatg aaggaaaaaa agggttggca aaatcagtag ctgattcttt
     2701 agtggaatat ctaaatgaag tattagctca agggcaaagc gatttgcttg attttttgga
     2761 tgaagacgat gacgaagctg tattcgagct tcactgggat aatcagcaac tagcagaatt
     2821 agtagaagta aaacagcaaa atgaagcaga tacttattta ccgtacccaa gttattaagg
     2881 agtgacagat atgaaatgga atgaagtaaa agtagaaact gaaagtgaag cagtcgaagc
     2941 agttgctaat attttgatgg aggcaggggc gagtggtgtc gcgattgaag attctttaga
     3001 tgttgaacat tttcagtcgg atcaatatgg tgagatttta gataaaaatg attttacatc
     3061 gctgaaagaa ggcgcactag tcatggctta ttttccggaa acgactttct taccggaaat
     3121 cttgcctttt atcaaagatc gtatcacaca attacctgag tttggtctag ctattggaaa
     3181 aaataaagta acagttagtg aagtagagga aagcaactgg gcaacagcct ggaagaaata
     3241 ctatcatccg gtgcgtatta cgcgaatgct gacggttgcg ccaagctggg aaaactacca
     3301 gacagaagat cctctagaaa aaattatcac actagatcct ggtatggcat ttggcacagg
     3361 cactcatccc acgacttcct tgacattgac agcactagaa acagtcttgc gtggtggtga
     3421 aacattgcta gatgtgggaa ctgggtcggg cgtgctaagt attgcagcaa aatatcttgg
     3481 tgcaaaagat gtctatgcct ttgatttaga cgaagtagcc gttcgttcgg cgaaagaaaa
     3541 tatggatatg aatgaaatag ccaaagatgt ccatgtatca gctaacgatc ttttgacagg
     3601 aatcgagatt gaaagtgatg taatcgttgc gaacatttta gcagatatca tcttattgat
     3661 gatcccagat gcttggcgtt tactgaagca aacaggcacg ctgatcgttt caggaatcat
     3721 tgaagagaag aagcaaatgg ttctggatgc catgttagaa caagggttta tcgtggatca
     3781 aattttccag caaaaagact ggtatgcaat tattttgaaa aaaccagagg tggaatagat
     3841 gcagcggtat tttttaaaag aatcatatga atcaaaagac tattttgttt tagaagggga
     3901 aaactatcac cacattgtac gggttatgag gatggaaccc gaggatgaag tttttttggc
     3961 attcaatgat caaatagcca ttgttgcaga aatcacggag attactgaag aggctgttta
     4021 tctgaaagaa attaaaaaag aagaaaatca aaaggaactg ccaagttcga ttaccatagc
     4081 gagtggctat cctaaagggg aaaagctgga ttggatcgtt cagaaatcca ctgaattagg
     4141 agcgcaagct tttcttggtt tcccggcatc agcctctgtc gttaaatggg atgcaaaaaa
     4201 acgtatgaaa cgacaacaac ggcttgaaaa gattgctcaa gaggcagcgg aacaatccca
     4261 tcggcagatc gtgccatcag tagagttatt ggaaacgaaa caagatttat ttgagcggct
     4321 agatgattat gacgtggtct tggttgctta tgaggaatca gcaaagcaag gcgaaaaatc
     4381 aaatgcagcc aaaattctta gtgaaacaaa accagatagt cgaatcttag ctgtctttgg
     4441 accagaaggt ggattatccc gcgatgaagt cgattatttt gtagaacaca aagggatact
     4501 ttgtggatta ggaccaagaa tccttcgtac agaaacagca cctctttatt tattaagtgc
     4561 tgctagctat caatgggaat taatgaatcc ttctttcagc taacgttctt cattaataaa
     4621 agagtatcga cagaaagaat tgaaaaaata gtcaacaatt tgtataatag aaacaattat
     4681 ttatacaatg gatacgaaaa atcgtttgaa aaagctttta aaaagcgaac atttccgtaa
     4741 aaaaacgtat aactgcacgg attttgggac aattatcaaa cagatatttg tcccgattcc
     4801 cgttttaccg aaaaaagaaa gggggatcga ttcctgtgac aaaagaagaa attttaacgg
     4861 gacccggtgt cataaagctc gttagtcaat acatgggtcc cgaacatgta gcatttgttg
     4921 agaaagcatg cgaatacgcg actgctgcac atgacggaca gtttagaaaa tcaggcgaac
     4981 cctacattat tcatcctatc caagtcgcag gtatattagc agatttaaaa atggatcccc
     5041 atacagtggc tacaggcttc ttacatgatg ttgttgaaga tacagaaatc actttagaag
     5101 atctgagaga agaatttggc gatgacgttg ctatgttagt ggacggcgta accaaattag
     5161 ggaaaataaa atataaatcc cacgaagagc agctagcaga aaaccaccga aagatgctgc
     5221 tagcaatggc tcaagattta cgagttatca tggtcaaatt agctgataga ctacataaca
     5281 tgcgtacgtt gaagcacctg cgagaagata agcaaagaag gatcgctcag gaaactttag
     5341 aaatctatgc gcctcttgca catcgtctag ggatcagccg gatcaaatgg gaattagaag
     5401 atacagcact tcgttatcta aatccaaaac agtattaccg tatcgtccac ttgatgcaga
     5461 cgaagagaga agaacgtgaa aaatacgtaa gtggtactgt tgaagatatt cgaatagcga
     5521 cagaagagct ggggattttt gcagaaatct atggacggcc aaaacacatt tattcaatct
     5581 atcgtaaaat gaaagatcag aaaaagcagt tcaacgaaat ttatgacttg ctagctattc
     5641 gagtgatcgt agattcaatc aaggattgtt atgctgtatt aggagcaatc cacacaaaat
     5701 ggaaaccaat gcccggcaga tttaaggatt atattgccat gccaaaagct aatatgtacc
     5761 aatctttgca tactaccgtg atcggtccgg caggaaatcc ggtagaaatt caaatccgaa
     5821 cacaggaaat gcatgaaatc gctgaattcg gggttgctgc acactgggcc tataaagaag
     5881 gaaaaaatga aaaagtagaa ccagatggta tgacgaaaca attaagctgg ttccatgaga
     5941 tactcgaact tcaagacgaa agctatgatg cttctgaatt tatggaaggc gtaaaaggag
     6001 atatctttag tgataaagtc tacgtcttca caccaaaagg agacgttact gagttaccaa
     6061 aaggatccgg accattggac tttgcataca gtatccatac agatatcggt aacaaaacca
     6121 ctggtgcaaa agtaaatggc aaaatggtgc agcttgatta caaattgaaa aacggagata
     6181 tcattgagat catgacttct ccaaattcat ttggcccaag tcgcgactgg ttgaaattag
     6241 ttgctactag caaagcaaga aataagatca aacgtttctt caaagcccaa gatcgagaag
     6301 aaaatgtgat caaaggccac gaatccgtgg tcaaatgtat tacagatcta ggatttacgc
     6361 ctaaagatat tttgacgaag aacaaactgc aagaagcact cgatcgtttt aattatcaaa
     6421 cagaagatga tctctatgca gctgtagggt atggagaagt tagccccttg acgatggcca
     6481 atcgtctgac tgaaaaagaa cgtaaagaac aaaaaatcga gcagcaaaag caagaagcag
     6541 aagaaatcat gaatcagccg aaaaaagaac ctgacaaaat gaaagtacgt catgaaggtg
     6601 gcgttgtcat tcaaggggta gaaaacttac taattcgtat cagtcgctgc tgtaatccga
     6661 ttcctggtga tgatatcgtt ggttatatca ctaaaggcag agggatatcc attcatcgtc
     6721 gagattgtcc gaatgttcag cctgacaaac caaatgtagc agaacgtttg attgaagtcg
     6781 aatgggaaga tacatcgaat acacgaaaag agtatgatgc agatttggaa atttacggct
     6841 ataatcgttc aggcttattg aatgatgtac ttcaaacagt caatgcgcta acgaaaaatc
     6901 tcaacagcgt tgaagcacgg acgaataaag ataaaatggc gacgatccat ttgacggttg
     6961 gtatccagaa tttatcccac ctaaagagta tcgtggataa aatcaaagca gtacctgatg
     7021 tctacagtgt acgccggacg aatggatagg aggacagata tgagagcagt tgttcaacgg
     7081 gtaagtaaag ctgctgtgtc agtcgatcag caagaaattg gaaaaattgg ctacggccta
     7141 cttgttttat taggcattca tgaaaccgat acgcaaaaag atgtggacta tctggtaaaa
     7201 aaaattgcac aaatgcgtat ttttgaagat gaacaaggaa aaatgaatat tagtgtagaa
     7261 gatgtgggag gacaaatttt aagtatctct caattcacac tgtttgcgga tacgaaaaaa
     7321 ggaaatcgac caagctttgt ggaagccgca cgaccagaaa cagctattcc tttatacgaa
     7381 tcattcaatg aaggaatcag aagtcgcggc atcacagtcg ctaccggaca attcggagca
     7441 gatatgtcgg tttctctcgt caatgatgga cctgtaacga tcattattga ttctaaaaac
     7501 aaataaagta atctggtttt cactagtaaa aactatgaaa cacgtcgctt aagttagact
     7561 gataaactct aacttaagtg gcttttttgt ctgcaaaaag cgcttctttt gtcagcagtc
     7621 ggaaaaaaag ttaagcttaa attatacaag ttatttttta taggaggttg ctgaagatga
     7681 aagaagtacc agtagtagat atcaaaatca cagatttaaa aaaattgtat caaaaaggat
     7741 acaacatgtt ggaggagtta cgccatgaag cggacgctcc cgtcgtcaaa gcaaagattt
     7801 tcaataaaga agctatcacg atctacggat catcagcagc aaaagtattt tatgatccaa
     7861 ggaatttcaa acgaaaaggt gctatgccaa aattagtatt gaagacactt tttggtcaag
     7921 gtggagtaca gactttggat ggagctgctc atcaccatcg gaaaaatatt tttatggatc
     7981 taatgacacc ggaaagaatg gaagattatc atcgtatctt agataagaat ctgacacaag
     8041 cacttgaagc acagcatggt caatttgaac tatttgattt aagtaagatg gttttcttta
     8101 catctatttg cgagtgggca ggtatcaatt tatcagcgat ttctaaagat gaggtcgaaa
     8161 aattagcaga atatcaaatc tctatgatca gcggaacgtt tacttctccg attgatcaca
     8221 taaaaggtgt cgaaaatcgc aaaaaatcag aaaaatgggc gcaaggcttg attgaagaag
     8281 ctcgacaaaa tcctgtggct ggaaaagaaa atgtggcatt atatgctttt gccaatgcca
     8341 cagatttaga tggtcagctt ttacctctgg aagtggcagc tgttgaatta ttgaacatca
     8401 tccgtccaac ggttgctcta actgtgtggg cggcattgat gggacatgca cttttcagta
     8461 gaccagattt gtatcagcaa ttaaaaaatg acttttcgac gttacaagat ccgtttattc
     8521 aagaaatgag acgctattat cctttcttcc caatgctacc tgcgatttcg ctgaaagaag
     8581 tagaagtaga tggttatcgt ataccagaag gcagttgggt tattttagat ttatatggaa
     8641 cagaccatga tgaacgaaca gttgaagcgc cagattcctt tatgatcaaa cggtatgtag
     8701 ggaaagcaaa agatatttcc tataaagaag aatatgagat gattgcacaa ggcgggggaa
     8761 attttcgaca aatgcatcgc tgtgcaggag aatggatcac tcttcatagt ctacgtgtgt
     8821 tttccgatca gctagtaaat aagtttgagt tctctgtacc tgaacaagat tggacgattc
     8881 cattcaatca gtttccgact tatccgaata gccgagcgct actttacaag aattagatta
     8941 gcataaactt atcgaaaaaa aggaagatca aacagtgctg atcttccttt tttcatatta
     9001 ctataaattg ataaggtcaa ttgatcaaaa tccacgaaat gcttcatcaa tggtttgata
     9061 atcttcattg gataaatgca aatctaaagc ttggacattg tcagatactt gctctggacg
     9121 cttagctcca ggtataacta cagacactcg tggattcttc atgtaccaag ctaaaacaag
     9181 ttgcgcagga gtggcttggt atcttttagc aatggggcga agtacatcga cagcggttaa
     9241 aatttctctg aaacgttctc cttggaaatc tggatttctt tttcgaggat ctccttcacc
     9301 aaattgtttt tcctcaccaa gttcatattt tcctgtcagc aaaccggaag caagaggaaa
     9361 atagggaaca aaactgattt tgtttttttc aagataaggg aataattctt tttcagcctg
     9421 ccgatggatc aaactgtatt tatcttctac tacatcgaca tatccatctg cattcgcttc
     9481 tttcaattgt tctaacgtga agtttgacac accgactgct cggatttttc ctgcttcttt
     9541 taattcgtgc aatgtagcaa ctgattcatt ttttggtgtg ctttcatccg gaaagtggat
     9601 ataaaaaatg tcgatgtaat cagtttgcag acgttttagt gcatcttcca ccgattgttt
     9661 gagaaactca ggagaattat caaatgttcg ccctttattt ggtacatgag cagcttttgt
     9721 agcgatgacg acacgtgagc gatcatattc tttttccttc agtacctcgc caatcagttc
     9781 ttcggaacgg ccgtttccgt aagcataagc ggtatcgatc aaattgatcc cgctattcaa
     9841 agctgtacga actacttgtt ttcctgtctc atcttccaaa ccgtcaaaaa gattatgtcc
     9901 tccaacggca tttgctccta atccaagctg ttctgcgtag acttgtgaat gtccgattct
     9961 tatttctgcc atagctgatt cctccttttt gtctagtcta aaacaaaata ccaaaaagct
    10021 gaactaaaac gtttttgtga ggggaaatag tcagatatct cttgactttg agactggttt
    10081 ttagtaggtt ataagtaata tcgaatgtca atgaaagagc gagtaagttt ttgaaacttt
    10141 acaagagagg atcatcgagg ctgaaaatga tctaaagttt cattagccta aagacactct
    10201 ggagatggac gagtgaaaag ccgtcccgcg tacgagcgtt attcgtaaga ggaaagagaa
    10261 atctttcaaa attaggtggt accgtgtatg aatttcattc acccttgttc atgatgaaca
    10321 agggtgattt ttttatttac atagctaaga aagggagaga aaactatgag tttccaaaga
    10381 ccaaaaggaa caaatgatat tttgccaggc gaatcagaaa aatggcagtt tgtcgaagaa
    10441 acagcacgtt tattattcag agattatcag tataatgaaa tgcgtacacc gatttttgaa
    10501 cactatgaag tgatatcccg aagtgtaggg gatacaacgg atatcgtttc aaaagagatg
    10561 tatgattttt atgacaaagg ggaacgccat gtgacgcttc gaccagaagg tacagcacca
    10621 atcgttcgtt cttttgttga gcataaattg ttcggcccag agtatgcgaa accgttcaaa
    10681 actttttata tgggaccaat gtttcgctac gaacgccctc aaaaaggacg gttacgtcaa
    10741 ttccatcaaa tcggagtaga agcatttgga tcagaaaacc ctgcaactga tgtggagagc
    10801 atggtgatgg cacttgattt cttcaaacag ttggggatca cgcaaatccg gctagtcatc
    10861 aattcattag gtgataaaga aacgcgtcaa gcctatcgtc aagcattgat cgactatctt
    10921 ttaccttttg aatcagagct gagtgaagac tctaaacgcc gccttcatga aaatccatta
    10981 cgcgtattgg acagtaaaga caaacgagat caaaaatttg tggcagaagc tccatctatc
    11041 ttagattatc taagcgagcc tgctcaaaaa cattttgata ccgttgtttc catgctggat
    11101 gcattggctg ttccttatga aatcgacagt aatatggttc gcggactaga ttattacaca
    11161 catacgattt ttgaaatcat gagtgacgca ccaaaaatgg gggcacaagc aacgatttgc
    11221 gcaggcggac gttatgacaa tctagtggaa gaactaggtg gtccttcaac acctggcttc
    11281 ggatttgcaa tgggtatcga gcgattactc ttgactatgg aggcagagga agtagttatc
    11341 cctgcattta atgagttgga tgcttacgtg gtcgcactcg gagacgaaac aaatatcgaa
    11401 gcattgaaag tggtccaagc gattcgaaac tttggttttt cagctgaccg cgacttcatg
    11461 aaccgtaaag caaaagcgca atttaagaca gcagataaag cgaatgccaa attagtattg
    11521 gtgatcggtg aagatgagct tgctagtcaa acgattaatg tcaaagcaat ggcaagccga
    11581 aaagaaaaaa gttttccatt agaagagata tatgaacatt ttgatgaagt ctatgatgaa
    11641 atgactttat tcgagtagga ggaaaaaaat aaatggcaca aagaacgatc tattgcggat
    11701 tagtttccga acaacaatta gaacaaacag ttacattaaa aggatgggta caaaaaagac
    11761 gagaccttgg gggagtaatc tttatcgacc tacgtgatcg tgaaggaatc gttcaaatcg
    11821 tctttaatcc tgaaatcaac aaagaagcat gggaaatcgc ggataaatgt cgcagtgaat
    11881 acgtgatcga agtgactggg gaagttagaa gacgcggaga aaatgcagta aatcctaaaa
    11941 tggccactgg tgaattcgaa gtgattgcaa gtgaaatcgt gattttgaat caagcaaaaa
    12001 ccccgccatt tcaaattgaa gatgatcaag caatcagtga cgaaatacgt ttgaaatatc
    12061 gttatttgga tctgcgtcgt ccaaaaatga ctaaaaacat caaattaaga aatgcgacaa
    12121 caaaagcgat tcgtcattat ttagatgata atgactttct cgatatcgaa acaccttacc
    12181 tagggaaatc tacaccagaa ggcgcaagag actacctagt tccttcacgt gtccatgctg
    12241 gacatttcta tgcgttgccg caatctcctc aattgtttaa acaattgctg atgaatgctg
    12301 gattcgaccg ttattatcaa atcgttcgtt gtttccgcga tgaagattta cgtggagacc
    12361 gtcagcctga atttacgcaa gtagatattg aaacaacgtt cttaacagca gaagaaatcc
    12421 aaacttacac agagggattg attgcgaagg taatgaaaga agttcgcggt atcgaagtca
    12481 cattgccatt tcctcgtatg acctatgatg aagcaatggc tcgctacggc agcgacaaac
    12541 cggatacacg ttttgatatg gaattgatcg atctaagcga tacagtcaaa gaagtagaat
    12601 tcaaagtctt ccaaatggct cttgaaaacg gcggtgtcgt gaaagcattg aatgctaaag
    12661 gcgctgcaga tcgctactca cgaaaagaca tggatcagtt aggtcaatat gtaggacaat
    12721 tcggagctaa ggggcttgca tggctgaaag tagaagaaga cggattgaaa ggtccaattg
    12781 ctaaattcat gggagaagca acagaagcaa tcattaaagc aacagatgca aaaccaggtg
    12841 acttgctgat gtttggcgca gataaaagtg aaatcgtcgc tgctgcatta ggtgctatcc
    12901 ggacacgttt aggaaaagaa ttaggactaa ttgatgaatc gaaattcaat ttcttatggg
    12961 taactgattg gccacaattt gaattttcag aagaagaagg tcgatatgtc tctgctcacc
    13021 atccattcac gatgccaaaa gaagaagata tcccgttatt ggctgaagac cctgcaaaag
    13081 tctatgcaga agcttatgat atcgtcttga acggttatga actaggcgga ggttctatcc
    13141 gtattcatac tcgtgagctt caagaaaaag tactagaaac tctaggcttt acaaaagaac
    13201 aaatggaaga acaatttggt ttcttgttaa cagctttaga ttacggattc cctccacatg
    13261 gcggtattgc attaggattg gaccgttttg caatgttatt agccggagaa gataatattc
    13321 gtgaagtcat tgcctttcct aaaaacggaa aagcagcaga tgtgatgagt gatgcaccaa
    13381 gtatggtttc gcctttacaa ttgttcgaat tgaatattga tgtaacgaat atagaagaat
    13441 agccgataaa aaggtaaaaa ggatttgtcg aatacgcttc ttttgacaac cttgatttaa
    13501 ttgaaaaaca ctttataatg aagccataac atataaagcg gggcttttcc atgaaaaaaa
    13561 cattcgaaaa ttgggcttat catgattaca cgaccaagct ttcggtatct atcgtttatg
    13621 cgttgatcgc atcagtcgca ttgaatttct tttatcagcc aggacatatt tattcaagtg
    13681 gtattactgg tctagctcag attttgacga ctttgtcaaa atcactgata ggattcgaag
    13741 taccggtatc aacaactttg tatttactga atataccttt gtttgtattg gcgtggttaa
    13801 aaatcgggaa gaagtttaca attttcactt ttatcacagt cactttgacc tccctttgta
    13861 tccatatttt accggaaaca gtcctatcta ccgatccaat catctgtgcg ttgtttgggg
    13921 gggctgtgat gggactaggt attgggctag cgttgagaaa cggcctatca tcgggcggtc
    13981 tagatatcgt gagtatcacg attcgtaaaa aaacaggaaa gtcgattggt tctatttcga
    14041 tctacttcaa tgcattgatc gtttttgttg ccggaattct atttggctgg caatatatgt
    14101 tttacagtac gttgtctatt ttcgtcagtg gaaaagtaac tgatgtcgtt tatactaaac
    14161 aaaagaagat gcaggtaatg atcgtaacga aacatccaga aaaagtagta aatgaaatcc
    14221 aaaactgtat gcgtagaggg atcacgatca tcaatgaagc agaaggtgct tataagcatg
    14281 ataagcagac agtattgatg acgatcgtta cacgatacga actgcctcta cttagagaag
    14341 caatgaaagc ttctgatcca caagcttttg taagtattgc agaaaacgtc cagatacttg
    14401 gtcgtttcta tgaggatggc atttagatcg agtttgacaa aaattcatga aatactgctg
    14461 tagacagacg ttcgtttgtc tgcagttttt ttctaccata gtagaatcag caaagaaaaa
    14521 ctgctttttt a
//
LOCUS       DAKBVG010000055        14530 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000055 DAKBVG010000000
VERSION     DAKBVG010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.055"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(292..987)
                     /locus_tag="OUD09_001174"
     CDS             complement(292..987)
                     /locus_tag="OUD09_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HCT9692454.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(1077..2177)
                     /gene="ychF"
                     /locus_tag="OUD09_001175"
     CDS             complement(1077..2177)
                     /gene="ychF"
                     /locus_tag="OUD09_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCT9692455.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(2186..2377)
                     /locus_tag="OUD09_001176"
     CDS             complement(2186..2377)
                     /locus_tag="OUD09_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HCT9692456.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(2398..3288)
                     /locus_tag="OUD09_001177"
     CDS             complement(2398..3288)
                     /locus_tag="OUD09_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCT9692457.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(3275..4042)
                     /locus_tag="OUD09_001178"
     CDS             complement(3275..4042)
                     /locus_tag="OUD09_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HCT9692458.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(4054..4767)
                     /gene="rsmG"
                     /locus_tag="OUD09_001179"
     CDS             complement(4054..4767)
                     /gene="rsmG"
                     /locus_tag="OUD09_001179"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCT9692459.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(4999..6900)
                     /gene="mnmG"
                     /locus_tag="OUD09_001180"
     CDS             complement(4999..6900)
                     /gene="mnmG"
                     /locus_tag="OUD09_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCT9692460.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(6917..8314)
                     /gene="mnmE"
                     /locus_tag="OUD09_001181"
     CDS             complement(6917..8314)
                     /gene="mnmE"
                     /locus_tag="OUD09_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCT9692461.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            8613..8996
                     /locus_tag="OUD09_001182"
     CDS             8613..8996
                     /locus_tag="OUD09_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9692462.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(9164..9922)
                     /locus_tag="OUD09_001183"
     CDS             complement(9164..9922)
                     /locus_tag="OUD09_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HCT9692463.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(9940..10758)
                     /locus_tag="OUD09_001184"
     CDS             complement(9940..10758)
                     /locus_tag="OUD09_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCT9692464.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(10755..11111)
                     /gene="rnpA"
                     /locus_tag="OUD09_001185"
     CDS             complement(10755..11111)
                     /gene="rnpA"
                     /locus_tag="OUD09_001185"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCT9692465.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(11229..11363)
                     /gene="rpmH"
                     /locus_tag="OUD09_001186"
     CDS             complement(11229..11363)
                     /gene="rpmH"
                     /locus_tag="OUD09_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCT9692466.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            11886..13220
                     /gene="dnaA"
                     /locus_tag="OUD09_001187"
     CDS             11886..13220
                     /gene="dnaA"
                     /locus_tag="OUD09_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCT9692467.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            13418..>14530
                     /gene="dnaN"
                     /locus_tag="OUD09_001188"
     CDS             13418..>14530
                     /gene="dnaN"
                     /locus_tag="OUD09_001188"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCT9692468.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLIT"
BASE COUNT         4021 a         3063 c         2615 g         4831 t
ORIGIN      
        1 gttgggttta ttaccgcaga tgcaaacttt tgaacaccgt ttagtggaga ggctgtgaaa
       61 aagctgctta gtttcgagta gtaagaaaaa ttttgtgaaa atggctttcc acatttttgc
      121 ataaattgga cttaataccg agaaactggc ttttgaacac cgtttatcgt aaaaagggac
      181 tgtgacaaga cttttgtcac agtcccttac tattatttaa ctatggataa acaaaatgag
      241 gttctttact acccctagat atctagcaaa atagctgatc tttttgctag tttatcttct
      301 tgggacggat aaacttccaa taatcccgta ttttttcttc aagtagtaac ggattgctaa
      361 agcagttgcc ccgataatga taactactac tggatctaat actggattga taactggtgg
      421 caatagtgtt gtcgcagaaa acagtacgac ccaaagaagt gtcgttccac ctaggataag
      481 caatgtctta cccattcctg gtttcttgct tcgatctgct ccgggatatt catattgata
      541 gatgtatttg tacatcatat aaaagaccca gccaccgatt gcagaaccta gaacgagtgt
      601 agaaaagcca taagctggcg ttgttccacg tgaaaacaat ccggtaatcg caatcatgat
      661 acttaataga ccaagcaaca gcaacgtatt atctaaccac atcaataaag gggtggatga
      721 attttttact tctgctggct tattgatgat tgcttccgtt ctttcagaga ctgtcccaaa
      781 caattgacgg gctgttcttc cgcctttttg ttctgcaact aaagtaggca gaatgtcatg
      841 aagggctttt actttttctt cttcagacaa attcgctgct tctaaagatt ttttcaaatc
      901 aaaaatgtac tgttgatttc ttttggttaa ttttgtttct aaactattgt tttcttttac
      961 gattgctcgt aattgttctg gttccattat ctaatagtcc tccttagccg ttccttttct
     1021 caacggtcgt cacaaaagac attctaccat attctacagc tagtttgata gcccttttac
     1081 acattaaagc ggaataacat tacgtcgccg tcttgtacga cataatcttt tccttctaat
     1141 cgaacacgtc cagcttcttt tgctgcatgc atgtttccgt aatgatccaa gtcttcaaaa
     1201 gagacagttt ctgcacggat aaatccgcgt tcgaaatctg aatggatgat tcctgctgct
     1261 tgtggtgctt tgattccttt tttgaatgtc caagcacgta cttcttgttc cccagcagta
     1321 aagtaagtag ctaagcctaa caaatcataa gcagcacgga tcaactgatc caaacctgat
     1381 tcttcgattc ctaaagcttc taaaaattcc gctttgtctt cttcatctaa ttctgcgatt
     1441 tcttcttctg cacgagcact gaccacgatc acttctgcat tttcttctgc agcaaagttg
     1501 cgcacttctt gtacataatg attcgcgtct gcatctgcta cgtcatcttc tgagacattg
     1561 gctacataaa gaattggttt tgtcgtcaat aaaaatagtg atttaacgat tttttgttct
     1621 tcttcagtaa attcgatcgt tcgggcagat tttccttctt ccagtacagg tttgatttta
     1681 tccagtacag ctagttctgc cactgcatct ttatctttgg ttttagcaac ttttgctaca
     1741 cgagtatagc gtttgttgat agattctaag tctgctaaca ctagttccaa gttgatcgta
     1801 tcaatatctt ctagtggatc aacacgtcct tctacgtgag taatattatc atcatcaaaa
     1861 cagcgaacaa cgtgacaaat agcgtctact tggcggatat ggcttaagaa ttgatttccc
     1921 aaaccttctc ctttacttgc gccttttacg attccagcaa tatctgtaaa ttcaaaagtc
     1981 gtcggaacag tttttttcgg atgtacaagt tctgttaagc gttgcaaacg ccagtcaggt
     2041 acttctacca tccctacgtt ggggtcgatc gtcgcaaagg gatagtttgc tgcttctgct
     2101 cctgcttttg tgatcgcatt aaacaaagtt gattttccta cgttgggcaa cccaacaatt
     2161 ccagcagtca atgccataaa tttattcact ctttctctgc atttttttca attacttttt
     2221 ttaatttctt ttcaaaatct cgacgggtca tcatgacgat atgtccgcaa ttcatacatc
     2281 tgattttgat atctgcgccc attcgaatga cttcccagcg attcgtctgg caagcgtgag
     2341 gttttttcat ttcaacgata tcgtgcaagt cgtacataaa aatagcctcc tttttgttta
     2401 ttcttcatca aattgaatat tcaagatatc aagaattcga gtcaaatctt tttgagaaag
     2461 atattcaatc tcgatcttac ctttcccatt tttttcttgg atagctacac ttgtaccaaa
     2521 tttatccatc aaacgatctt cgctttctcg gatataatat ggtttttcct ttgctagcct
     2581 tgggattttt ttcttttctt ttccatcatt ttgatttaac tcagaaacaa gctgttccag
     2641 ttggcggaca gttaaatttt cagttactgc acgattggct aatttcaaga tcaatgtttt
     2701 attttttaaa cctaacagcg tacgggcttg tcccatagat aaacgctgat cttgtaccat
     2761 ttccttgaca agatccggta aagaaagcaa gcgcaaataa ttggcgatat aaggtctgct
     2821 ctttcctaaa cgactagcta cttctgcttg tgttagtttc aggtttttca tcagcatctc
     2881 atatgcttct gcttcttcta acggattcag atcttctcgc tgcaagtttt ccagcactgc
     2941 tacttgcatc atcgcttctt cgtcaaagtc tctaacgata gcagggatcg tttctttttc
     3001 agctagtttg gacgctcgaa aacgtcgctc ccctgcaata atctcatatc cttttactgc
     3061 ggaacggcgt acgatgatgg gttgaaagac acctgattgt tcaatagaat tagctagttc
     3121 ctgcaaagat acttcttcaa atgttttacg gggctgatac ggatttggtc gcagctcatt
     3181 caatggtagc tggacaacgg tatcatcttt tacatccacc gtctctaagt tttctaaatc
     3241 ttgaaacagt gcatcgattc ctcggcctaa tcctttactt ttcttcacga cttaccactt
     3301 cctttgccaa tgcttggtag acttcagctc ctcgagaacg tggatcgtag tcgatgatcg
     3361 gttttccatg actaggtgct tctgataaac gaatatttct tggaatgatc gtttcgtaaa
     3421 ctttttcgcg gaagtattta cgtacttctt ctacgacttc atttcccaga tttgttcgtg
     3481 catcatacat tgtcagtaag actccctcga tctctaattc tggattaaag tgtttttgga
     3541 ctaaacgaac cgtattcagt aattggctta atccttccaa tgcgtaatac tcacactgaa
     3601 caggtatcaa aatcgaatca cttgctgtaa atgaattgat tgtcagatgg ccaagagaag
     3661 gcgggcaatc gatcaaaata taatcatact gactgcttac ttctgctaaa gatcctttta
     3721 atcttgattc tcttgccatc attgacgtca attcgatctc tgctcctgct aattggagag
     3781 tcgcaggcac aatactcaaa ttttcgtgtt cagtgatcaa agttgcctca tcaattggca
     3841 attcattgac tagaacatca tagatgtctc ttgtcacatc aggttttctg atcccgacac
     3901 cactggtagc atttccttgt gcatccatat ccactagcaa tacttttttg ccaaggctag
     3961 caagacaagc acctagattc accgtcgtcg ttgttttgcc gacgccacct ttttggttcg
     4021 caacagatat tatttgtgcc atctcttctc ctcctatttg attggctgct tattaggcag
     4081 tccgggcttt cttgggtatt tctttggggt tggtttcttt ttttgaataa cgatgatatg
     4141 ccgttgatca gagacattcg gcaaggtaac cgcctcttct ttgaggaatt taccacctaa
     4201 taaagcaatc gcttgttttg cttctgtcaa ttcctcttca ctttttgctg ctttcaatgc
     4261 ataaaaatag ccttcttgtt tagccaaggg caaacacaat tccgctaaca cattcaaacg
     4321 agcaaccgca cgagcagtga caaggtcaaa tgatgcacga aagtgaggat tttgcccaaa
     4381 tgtttctgcc cggtcatgat ataaggaagc atcgattcct aataatcggg ttaactcaga
     4441 taagaaggtt attcgtttat tcagagaatc aacgatcgtt atctgcagtt ctggaaaggc
     4501 aatttttaaa ggtatgctag gaaaccctgc ccccgaacca acatcgcata aagataaagg
     4561 ttgcgtaaag tctacttcaa atgctaatgt aagtgaatcg taaaaatgct tcagatatac
     4621 gtctttttca tccgtaatag ctgtaaggtt catcttttcg ttccattcga ctaatgtccg
     4681 ataataaata gcgaattgat tcatttgatg atcggtaagc tctatccctt tttctgccaa
     4741 ggattggcga aattcttctg gtatcataaa tattcctttc attgtgaaac agcaattgtt
     4801 tcacagattc tctccctact ttaacgcaaa atctctagac atgcaatcct tccatatgga
     4861 ttcttaaaaa tttgaaaagt aagaaaagcc gatagacttt ttctttctag tatgactcat
     4921 aaaatataca cagaagagag aactagcttt gagcaatttc tgcaaaaact agttctctct
     4981 tctatttatt attacgtatt acgctggttc actgactttc gcaatcttcc cttgttcgat
     5041 atagaccatt aggatactta ggtctgcagg gtttacgcca ctgattcggc tagcttgggc
     5101 aattgtttct ggttggattt tctgcaattt ttgttttgct tctgttgcta atccgttgat
     5161 tgctgcgtag tcgatgtttt ctggaatctt tttcgcttcc atgcgtttta atttttctac
     5221 tttttccatg gcttttttga tgtagccttc atatttgatt tggatttcta cttgttcgat
     5281 ttcttttcgt gtcaattctt cattttctgg aataaaccgt agaagttctg cataagtaat
     5341 ctcaggacgt cttaaaaagt cgcttgctag tacaccatct tttaatggtg ccgcattaat
     5401 cgttgctaaa aagtcttgga cttcttctgt tggtttgatt cgtacattca tcaaacgttt
     5461 gatttcttgt tcaactgctt cttttttctt caaataattc ttgtattgat cttcttttac
     5521 taagcctaca cggtgaccca tttctgttaa tcgcaaatct gcattatcat gacgtaaaag
     5581 cagacggtat tcagcacgtg aagtcagcaa acgataaggt tcgttcgttc cttttgttac
     5641 caaatcatcg atcatgacac cgatgtaacc gtcacttcgt ttgatcacta aaggttcttt
     5701 tccctgaact ttcaaagctg cattgatacc ggcaatcagc ccttgtccag ccgcttcttc
     5761 ataaccgctt gtaccatttg tttgtccagc tgtataaaga ttttcaacga ctttagtttc
     5821 taatgtcgga cggagctggt gtggctctac tacgtcatat tcgatagcat atcctgtacg
     5881 catcatttct actttttcta atccagcaat agaatgaagc atgttgactt gaacatcctc
     5941 tggtaaagaa gtagataaac cttgaacata gacttcttcc gtatttaagc cttctggttc
     6001 caaaaataat tgatgacgtg gtttgtctgc aaaacgcacg atcttatctt cgatggatgg
     6061 acaatagcga gcgcctaccc cttcaacgat ccctgtaaac attggggcac gatgtaagtt
     6121 gtctcgaata atttggtgag tcgtctcacc tgtataggtc agccagcaag attcctgatc
     6181 tagtttatag gcactatctg gtgtgctgaa gctaaaatga ttcggctctt tgtcaccagg
     6241 ctgctcttct gtaacagaat agtcgatcgt acttgatttt actcgaggcg gtgttcctgt
     6301 tttgaaccgt tcgatagcaa agccaagttc ttttagatga ttcgctagtc caatagaagg
     6361 ttgagagtta ttcggacctg atgaatattt caattcaccg ataatgattt ctccgcgaag
     6421 tgctgttccg gctgtaataa caactgcttt ggcccggtag ctcgctcctg tggacgtcac
     6481 tactccatga caaacaccat cttcgacgat cagttcttcg acgattcctt gtcttagcgt
     6541 caaatttggt tctttttcga tcgtatgttt catttctgtt gaataagcat gtttgtctgc
     6601 ttgtgctcgc aatgctcgga cagctgggcc ttttcctgtg ttcaacatac gcatttggat
     6661 ataggtttta tcgatattct tacccatttc tccgcctaac gcatcgattt ctcgaacgac
     6721 aacgccttta gctggtccgc ctacagaagg attacatggc atgaatgcta ccatgtccaa
     6781 atttattgtc agtaataatg tttttgatcc catacgtgca gcagcaagtg cagcttctga
     6841 accggcgtgt cctgcgccaa caacgatcac gtcatacgca tctgcttcat aatgttccaa
     6901 tcgtttgttc ctccttttat tttcctaaac aaaattgact gaataattga gtgattagct
     6961 catcttgtac actgtcgccg acaacttctc ctagatagtc ccagcaacgc gtcatatcta
     7021 tttgaacgag atccacaggc attcctgaat caatacctgc aatgacttct tcaagtgaga
     7081 gggaagcttt ttctaacaac gcgatatggc gggtattaga aacataagta gcgtctcttt
     7141 ctcctgtttc tccgctgaag aataaatcag aaatagctga ttccaaacga tctagtccgt
     7201 cgtttttagc tacggaaatt gaaaaaactg gttcattttc gatcaatttc ttcaattttt
     7261 cttgttccag ctgtgctggc aaatctgttt tatttagaag aatgatccgt ttcaatccac
     7321 ttgtcgcttc caataattgt tcatcttctg ctgtcagtgg ttcgctttgg tttagaacaa
     7381 gtaagatcaa gtctgcttct gctaatgctt ttcggcttct ttcgaccccg atccgttcca
     7441 ccacatcttc tgtttcccga attccagctg tatcgattag cttgagcggt accccccgaa
     7501 cattgacata ctcttcgatt acatctcgag tcgttcctgc aatatccgta acgattgctt
     7561 tttcttctct taacaaatgg tttaacaaac ttgatttgcc gacatttggc cggccgatga
     7621 ttgccgtact taacccttcc cgcagaacct tcccttgctt ggatgtagca agtagcgctt
     7681 ggattctttg ttggatcatc tgcgcttttt caagcaataa cttcgtagtc aattcttcga
     7741 catcatcata ttctggataa tcaatattta cttctacttg tgctaatgtc tctaaaatct
     7801 cttgtctcaa tgagcggatc aaagcagaca gattgccgtc taactgatta agagctagtc
     7861 ccattgcttt gtctgttttt gcccgaatca aatccatcac agcttctgcc tgtgaaaggt
     7921 caacacgacc attcaagaat gctcgttttg tgaattcgcc tggctcagct aatcttgcac
     7981 cttctcttaa caatagctgc aaaatttgat tgactacaac gatcccaccg tgacaattga
     8041 tttctactac atcttcccgc gtaaatgtct ttggtgctct catcacggag accatgactt
     8101 catcgacaag ctgattgctc ttgggatcaa cgatatgtcc ataatggatc gtatgacttg
     8161 gtacttctga taatcgtttg tttccagatt gataaacttt atctgctagt tgcacagctt
     8221 gatcgccact taaacgaacg atactgattg caccttctcc aggtggggta gaaattgccg
     8281 caatcgtatc aaattctaaa gtaatttcag ccattttttc acttcctttt tcattttgcg
     8341 cacaaaaaaa gtgcccactc caccctttat gcacaataaa cggtgaactt cgcactttga
     8401 ctgcttgttc ttgattaaat tctggtcata gattatcaca cttgctataa ggaatcaagt
     8461 gtttaaaaca attagatgaa gttgagcctg ttctgcttcc attaccttat atttaattgt
     8521 ccttggatgt ctctttgcgt tctttggttt attcgaagag aagatactgt ataaacaagg
     8581 ctttatcttt cttaaaaaaa acggtacact aaatggtagc gctatctaaa ctatacactt
     8641 ttgaaaggag aaaatatgtg agcccgttca gatctgctgc attggaagaa gaaagtgacc
     8701 ctcggatcaa cgatgtcgtt ttatctacag ggaatatcaa ccagaaatat atcgtccgcg
     8761 atatcgtttt tgttgtggaa tgcttagaag ctgatttgtt tgacccatct tttcccaaag
     8821 atgacctttt cttaacaacg aagaggaaat taaagacgaa ggtacttgaa tatggtgcga
     8881 atgcagtaat caactgtcat tttgagtatc agcaaaaaaa acgcgacgga aagtattttc
     8941 tggaactttt tgcttatgga acagtgatcc aatttatcca aacaacgatt ggttaaatga
     9001 aacagacaaa aaaagcggat caaaactgaa gacagtttcg atccgctttt ttctatcaaa
     9061 gttagtgact acttcatcgt atactctgat aagaatgatt tctagtaaaa tatacttggt
     9121 ttgaaaaact ttgtcaacgt gatttattca aaaagtaatg gaatcaataa tattttttag
     9181 caggttctac tactaaataa cgataaggtt cctcaccttc cgaatgtgtt tttacatgtt
     9241 catctttgct taatgcagca tggatttgtt tacgttcaaa tgcaggcatt ggttctaaga
     9301 aaacaggctg ccctgtccgt tttacttttt cagccgtgcg atcagctaaa cgctgaatga
     9361 tctcttggcg cttttcacgg taattgccta cattgactac tacagacagc ttatttcttg
     9421 ctaaacgatg gatgaagact tgtgccagat attgcaatgc attcagtgtt tttccatgtt
     9481 tcccaatcag tatgccttgc ttttgtgttt ctaaatggaa aacaaccata ttatttgatt
     9541 ctctttccat tttgacaaga gctggagcat caagctgttt tgaaatttca gtaagataaa
     9601 ctgccaattc cgttaaagct gcttcatcac ctagctcact tacttcttct gttttttctt
     9661 caagaatcgt ttcgttgact acctcgatct cagctgttgg ttcttcttcc aaaatttctt
     9721 ctacagtctg ttctactgtt tcagaaactt tttcgctgat acttggctgg atcgaaagcc
     9781 gtgcattctt tcgacccatt cctaggaaac ctttttttcc ttcttccagt atttcgattt
     9841 cgacttggtc tttggccaaa cccaaatcag ccagacctag ttggacagct tcgtctattg
     9901 ttgcagcttc ataaatcggc attagcatta cctcccctat tatttccgtt ttttcttagg
     9961 attcattgct ttcttaagag cacgttctct ttcgcggatt ctccgtgctt cttcttcacg
    10021 ctcttttcga attttgaaag ggttgttgat cagcagcgtt tgtacaactt ggaaggcatt
    10081 ggaaactacc cagtataatg aaagaccgct ggctaaattg acacccatca ctaaaatcat
    10141 tacaggcatc acgaaattca tgatcttcat tgatgcattc gattcaattt gactcatgct
    10201 agataaatac gtacttgcaa aagtaaagat tgctgctaaa atcggcaaaa taaatgttgg
    10261 atccgcgttc cctaagttca accataagaa atgcccttgt ttcaactctg gtacacgtga
    10321 aatagattgc cataacgcca tcaagattgg catttgtact aataaaggaa gacaaccagc
    10381 atatggattg acgccatttt ctgcatataa acgctgctgc tcttctctta acttactttg
    10441 tgtttccgga tctcttgaac tatattgttg ctgcagtgct tttaatttcg gttgtaattc
    10501 ttgcgtttta cgcatgctct ttgtttggaa atgcatcaac ggcaatagga tcacgcggat
    10561 gatcagtgta aataaaatga tccctatccc tgcattacca aatgacaacg ccttgatcgc
    10621 ctcagcaaag taataaacaa tataacgatc ccaaattcct gtactctgtg cgctgacttc
    10681 cccagttccg caaccggata agacgatgac caaactaacg ataccggcca tcaaaagtaa
    10741 acgtttgtat ttcttcactt actcttttcc tctctgtcga ttatttttgc aagttttaag
    10801 acgtggacta aattggagcg aacctcatca taagtcaacc ccttcacact tggtctagca
    10861 atcacaataa aatccaactc cggatcaatt tcttctttta tagaataaac tgctgctcgg
    10921 atttttcgtt tgacttcatt gcggtttacc gcgttgccga ccttttttcc tacagataaa
    10981 ccaactcgaa aatgaggctg tcccggtttt tccaaacggt aaacgacgaa tttgcggtta
    11041 gcaaaagaat ctcccgtatg gaacacggct tgaaactctc gttctttttt tactcgatac
    11101 gcttttctca ttagttttct ttccttttct atctctcact tacgctcata atcataccat
    11161 aattacgatt gtcctttcat catttcccta aaaaagagta taaaaaaaac cactgaaacc
    11221 ccagtggcct aagctgaaag aacttttctt cctttgcgac gacggctagc taacacacga
    11281 cgtccatttt tagtactcat acgtttacgg aatccgtgaa ctttttgacg tttacgttta
    11341 tttggttggt atgttctttt catatattcc acctccaaaa tgttcttatc tattttataa
    11401 acacagacat actttgatag tataacgaca aagtagtgat tttgtcaata taaaatagaa
    11461 gatcgattgt taaaacttat ccacaagatt atttttttct atgggaatcc tatgtggaaa
    11521 aagttatgca cagggattat ccacagataa taaaaggctg gttttacaca gaaaacacac
    11581 gtgtaaaaaa agtaatccac aatcaggcga tttcctgtgt attccgtctc ctaactattg
    11641 gtatatctgt attttttatc cacagacgca agaggaaatc tattggtttt tacgagtttt
    11701 ccacctgtgc attagtggtg tggaaaactt tttttgacaa ctgtgttttt ctcttttttc
    11761 tttttgtcga ttgtgtggat aattgtaatt aaaaaatttt tttcttcggt ttctgtggaa
    11821 aactattgaa aaaaatgcta aattagtact agttattcac tttttaatga aggaggaggc
    11881 cttatatggt atccctcgat gctttatgga atgaattaaa agcaacatac caaaaggatt
    11941 tatcgcctgc tagctataat acatggatcg aaacagcgaa ccctcgaacc cttgatcaaa
    12001 accagctcgt tgttgaggtt cctagcaaga ttcataaaga atattgggaa aaaaacctgg
    12061 cgacaaaaat cgttgaagtc ggttatatgt tatcaggaaa tgagattatc cctcgcttta
    12121 ttactggcga agaagcagag caagaagaag ttatagaaga aaaaaatcca aaagttgtgg
    12181 cacctagtcc gctaaaaaaa gccatgctga accctaaata tacctttgat acgtttgtca
    12241 ttggtaaagg aaaccagatg gctcatgcag cagcgctcgt tgtagcagaa gatccaggtt
    12301 ctatttataa cccgctcttc ttttatggag gcgtgggact tggtaagact cacttgatgc
    12361 atgcaattgg tcatcaaatg ctgcaaagtc agcctaatgc caaagtaaaa tatgtaagca
    12421 gtgaaacttt tgctaatgat ttcatcaatt cgatccaaaa caaaacagca gaagagttcc
    12481 gtcaagaata tcgaaatgtt gatttactgc tagtggatga cattcaattt tttgcagaaa
    12541 aagaagctac gcaagaagag tttttccata ctttcaatgc cttgtacaat gaagggaaac
    12601 aaatcgtact aaccagtgat cggttgccaa acgagattcc gaaactgcaa gaacgccttg
    12661 tttctcgctt tgcatggggg ctgtctgttg atattacacc gccggatctt gaaacaagaa
    12721 cagcgatttt acgtaaaaaa gcggcggctg aacgtctaga gatcccagat gacacactaa
    12781 gctacatcgc aggacaaatt gattctaaca ttcgtgaatt agaaggtgcg cttgttcgag
    12841 tgcaagcttt tgctgcgatg aatagtgaag atatttcgac tagtctagct gcagacgcac
    12901 tgaaaacgtt aaaatcagga aaaggacatc ctcaattgtc aatcctgcag atccaagaag
    12961 aagtggcaaa gtattatcac atccaattga aagatctcaa aggaaagaaa cgcgtcaaat
    13021 ctattgttgt tcctagacaa atcgctatgt atttggcacg tgagctcaca gataattctt
    13081 taccgaaaat cggagctgaa ttcggtggca aggatcatac aacggttatc catgctcatg
    13141 aaaaaatcca gcagctgatg gattctagtg tcagtatgca aaatgaagta tcagaaatca
    13201 aaaacttttt actaaattaa tttgtcgatt ctatcaactg aaaatagcat gtggataaaa
    13261 gaaaaaaacg ttcaaaagtt atacacactt tttccacagc tgttttttcg tttctctctt
    13321 acgttttttg agttttccac agaatcaaca tgccctatta cttttattat tctttttaac
    13381 taataataaa taaacaaaca ttcaaaggag atacatcatg aaagttactt taaaccgagc
    13441 tagctttatg caggaattgc aaactgttca acgagctatt tcaagcaaaa ccacgatccc
    13501 tattttgaca ggtgtaaaaa tcacactgac acaagaaggt ttgactttga cggggagcaa
    13561 cgctgatata tcaattgaaa cttttttgtc tgttgaaaac gaaaaagcaa atatgcaaat
    13621 cgaatctact ggttccattg ttttacaagc acgtttcttt agcgaaatca ttcggagact
    13681 tcctgaagaa acatttactt tagaagtttt agaaaataaa caagtagcga tcacttctgg
    13741 aaaagcgaat tttatcgtaa atggattaga tgcagataac tatcctcatc ttcctgttgt
    13801 cgaaagccat aaccagatga aattacctgt acacgtattg actaaactaa tcaacgaaac
    13861 agtttttgct gtctcacaac atgagagtcg tccaatcttg acaggtgtgc atttcatttt
    13921 atctgataat tctttattag ctgtagctac tgattctcac cgtctaagtc aacgcgtgat
    13981 tccagtagaa caagcggctg atcattttga tattgttatt cctggaaaaa gtttgatcga
    14041 attatctcgc tcattaacaa atgaagaaga aatcgtcgaa atcagcatta tggaaaacca
    14101 agtgttgttc aaaacagaaa cgatgtattt ctattcccgt ttgctagaag gaaactatcc
    14161 agataccaat cgtttgattc cttcaagttt taatacagag gttgaatttt ctgttcctag
    14221 ctttttagcg gcgatcgaac gggcttcttt actttctcat gaaggccgta acaacatcgt
    14281 tcgtttatct attcgtccag atgcggttgt tttatacgga aattcgccag aaatcgggaa
    14341 agtagaagaa agcttgagtt atacagcaag cagtggcgat ccattagata tttctttcaa
    14401 tccggattat atgaaagcag cgttgcgtgc atttggagat atgagcatca aagtgaaatt
    14461 tatctctgct attcgtccat ttacattgga accaacggaa gatggcgttc aatttattca
    14521 gctgattact
//
LOCUS       DAKBVG010000056        14318 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000056 DAKBVG010000000
VERSION     DAKBVG010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14318
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.056"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(35..898)
                     /locus_tag="OUD09_001189"
     CDS             complement(35..898)
                     /locus_tag="OUD09_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCT9692469.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(1094..3235)
                     /locus_tag="OUD09_001190"
     CDS             complement(1094..3235)
                     /locus_tag="OUD09_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HCT9692470.1"
                     /translation="MMKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDA
                     AMQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIE
                     KNEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHRED
                     TKEEHAKELLKMISEKIAQENKVEPEHEAEKDTEKDISGVNAAGKESLKTNDLKTNDL
                     KTDIPEKTETLSEKQSEDEKSNGLPEADKKEMLEKADKKATESDGINNEADAETQSAS
                     EDSTALEAKIEKEENQPELIEAETEPITIVPNTEKTPQESNPEKKDLDKTQKKKSKKV
                     PYLALAAVLLLGGGGWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFI
                     RADMIHQDLSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDH
                     LADDIKLKADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVV
                     DSATRDQYKTAKEAVEKVKNTDLSAQLNEQLQQVDKMLTDKEKKEEEEKKKAEEAKKA
                     EKAKKAAEEQAKQAAQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARAS
                     DIADASNPAWTWAPGVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKS
                     ALMKGIGESALPFYIVTINCKTGWFGGNGSN"
     gene            complement(3452..4561)
                     /gene="rpoD"
                     /locus_tag="OUD09_001191"
     CDS             complement(3452..4561)
                     /gene="rpoD"
                     /locus_tag="OUD09_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCT9692471.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(4585..6426)
                     /locus_tag="OUD09_001192"
     CDS             complement(4585..6426)
                     /locus_tag="OUD09_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCT9692472.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(6608..8923)
                     /locus_tag="OUD09_001193"
     CDS             complement(6608..8923)
                     /locus_tag="OUD09_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HCT9692473.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(9108..9968)
                     /locus_tag="OUD09_001194"
     CDS             complement(9108..9968)
                     /locus_tag="OUD09_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HCT9692474.1"
                     /translation="MEKRQKMENQKVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(10027..10659)
                     /locus_tag="OUD09_001195"
     CDS             complement(10027..10659)
                     /locus_tag="OUD09_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HCT9692475.1"
                     /translation="MKRKRLSIIRILFYMIILTVIGAVLFLGGRTYRSVKRVESYQIE
                     ITTAAKNYHMENYENLIKAIILTESKGKGTDLMRSSESLHGEAEIIDNPADSIDQGVK
                     YLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHGGENTVELAEAYSKDVLSPLLG
                     NEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKMNEKIIETYEKVFK"
     gene            10826..11587
                     /locus_tag="OUD09_001196"
     CDS             10826..11587
                     /locus_tag="OUD09_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCT9692476.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            11613..14204
                     /gene="mprF"
                     /locus_tag="OUD09_001197"
     CDS             11613..14204
                     /gene="mprF"
                     /locus_tag="OUD09_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HCT9692477.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLVSVLPMIGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDP
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
BASE COUNT         3861 a         2980 c         2516 g         4961 t
ORIGIN      
        1 tataagctag ttaagaaaaa catgcaaggg attctcaatt agattttttg actagttttg
       61 tcacaccgtt ttcttgtgta atcaatcctt gtttcaaaag attacctaat gcgcgcttaa
      121 atgctccttt gctgatacca aaagctttca tgatttcttc tggatccgat ttatctgtaa
      181 agttgatttg ctgatctgca gaacgttcta aaatggttaa aatcatttgt gcatcatctg
      241 atatagcttc atatgctctt ggcttcaatg aaagattcag tacaccgtct ggtcggacac
      301 caatcactcg accttttatt tgctcgccta gacgaggttc attatatcgt tcagaaggat
      361 ggataaaccc aagatagaaa tcttccgtta aaacgtacgt acctgttaat ttcaagcggt
      421 atgctgttcc tatcacatct ttgtttttca tttcttctgt tccatatttg cttaaagatt
      481 tgaatatttt ttcatctgct aaactgcccc aaatacggtc ttttgcatca actctaaggg
      541 aaatcatcaa gcgatcccct tttttaggcc atagctctgt cattgtcggc aattcatcaa
      601 gagatacgac aaagtcttta tctggaagac cgatatcgac aaacgcacct aaatcttttc
      661 gtgtagcagt aacgattcca aaagcgtaat gaccgatacg gcttttagga atttgtgtag
      721 tgatccgatt ttcatgtttt tgattttggt agccgaatcc ttccactgct tcaccgattt
      781 tgtgttcacc ttctgatttg cttaagccaa aggtttgtcc gtttttttgg acaaagtagt
      841 ttttttcatt ttcatcaata ataatacctg taaatatagt tgctaataaa tcgttcattt
      901 ttttctccct ctatagtgaa aggttttctt aatacatttc tttgctatca taacataatg
      961 ttatctactt gaatagctga gccttggttt ggaaaggtga aaaaaggagt atgacaacag
     1021 ttgtgtcata ctcccctttt tcgaataaac ggtgttaaac ggcaggctca cagcctcttt
     1081 catttgccta aaattaattg ctcccatttc ctccgaacca tccagtttta cagttgatgg
     1141 ttacgatgta aaatggcaaa gcactttctc caatgccttt catcaatgca gattttgttg
     1201 aagtggcata tagattgtaa tagccttccc cattttcgat tctggctttt tctaatctgt
     1261 atcctccttc aacgatatat ccacgttgga tacatgtagc aatcactttg ctttggacac
     1321 ctggtgccca cgtccatgct ggattagatg catcggcaat atcacttgct ctggcagatt
     1381 tgattggctg attcgctgag tttgaagagg aaggggtcga ggatgccgct tcttgctgtg
     1441 ctgcttgtgc gaccgcagct tgttgagcgg cttgagctgc agctgctgct tgagcggctt
     1501 gttttgcttg ttcttcagct gcttttttcg ctttttctgc ttttttcgct tcttctgctt
     1561 ttttcttttc ctcttcttct tttttctctt tatcagtgag catcttatct acttgttgca
     1621 attgttcatt caactgagca gatagatctg tatttttcac tttttctact gcttcttttg
     1681 ccgtcttgta ttggtcgcgt gttgctgaat ccactacttt tccgtcttta aagactatag
     1741 ctgttttttc tttagctgtt tgcaattcag catattgatt tttagcttct gtttgtgctt
     1801 gttgaatcaa ttttccaaaa tctgtgttat ccgtttcgat tgcttggatc tctttatctg
     1861 ctttcaactt gatgtcatct gctagatgat catcgacgat ggcaggtgct atgaaccact
     1921 cattcacttt ttggatatct cggatcttag attggatatc ttggtaagtt gtttctaact
     1981 ctgaatatcc tttttcattc ttaagactgg ataaatcatt ttttagtttt gataaatctt
     2041 gatgaatcat atctgcacga ataaattgat ggtgatcatc tgtataaaag gcagcaagtt
     2101 tctcatgaag ttcttccatt tgttgctgtc tttttgcttc tgctgctact tcggcttcta
     2161 ctttttgatg atggtcataa taagcccagc ctcccccacc tagtagaaga actgctgcta
     2221 aagcgagata cggcactttt tttgattttt tcttttgtgt cttatctaaa tctttttttt
     2281 ctgggtttga ttcttgaggc gttttttcag tatttggcac gattgttatt ggttctgttt
     2341 ctgcttcaat cagctcaggc tggttctctt ctttttctat tttagcttca agagcagtgc
     2401 tatcttcact tgcagattga gtttcagcat cggcttcatt atttattcca tccgactctg
     2461 ttgccttctt gtctgctttt tcgagcattt cttttttatc tgcttccggc aacccattcg
     2521 atttctcgtc ttctgattgt ttttcagaca atgtttcagt tttttccggg atatccgttt
     2581 ttagatcatt cgtttttaga tcattcgttt ttaatgattc tttacctgca gcgtttactc
     2641 cactaatgtc tttttcagtg tctttttcag cttcatgttc tggctctact ttgttttcct
     2701 gtgcaatttt ttcactgatc attttgagca gctctttcgc gtgctcttct tttgtatctt
     2761 cacgatgtgc tttgatataa gcggatagta tcgaattttc ttcctcatta tcgacgattt
     2821 ctttcagttc agctactctt cttttttgtt ttgattcaag cgtttcttct tgattctttc
     2881 ctgattcttc cgaagtctca tgaacagttg ctttcttctg ttcatttttc tctatattag
     2941 ttacttgatc gtgagcaggt ggttcttcgt cttctggttc tgctttttcg aataattcca
     3001 ttacagattc tagaggtaaa tctttcaatt ccgaccattc gattggatcg tttaattctg
     3061 tatcaggaaa gactgcatct tcagttgatt tatcctgcat agctgcatct gtatttgttt
     3121 cttcaattga ttcttttatt tgctgatctt ctgcagttat ttgatgaccg cagtctggac
     3181 agatagtatt ctgctccatt tttttcgatc cgcaatttgg acattttttc atcacttagc
     3241 gatcggctcc tttactaggt ttacgctacc atttaatcta tcatattcta tacgtcacgc
     3301 caatagctta tttttaggat ttacttaaaa ataatgaatg aatttccttc ctatagaaaa
     3361 aaaagcttga gactgcgtag tctcaagctt tttttctttt aaaataaacc aaacagtttt
     3421 actgatttct cttctggtca atagttcatg cttattctaa gaaatctttt aattgttttg
     3481 aacgagaagg atggcgcaat tttcttaatg ctttagcctc gatctgacga atacgttccc
     3541 gagtaacacc aaatactttc cctacttctt ccaatgttct cgtccggcca tcatctaaac
     3601 caaaacgtaa gcgaaggaca ttttcctccc ggtcagtcaa tgtatctagt acatcttcca
     3661 attgttcttt tagtaactca taagctgcgt gttctgctgg acttgttgct tcctgatctt
     3721 ctatgaaatc acctaagtga gagtcatctt cttcaccgat tggcgtttca agtgaaacag
     3781 gttcttgcgc gattttcaag atttctcgga ctttttctgt tggaagatcc atttccgcac
     3841 cgatttcttc tggcgtaggt tctcttccaa ggtcttgcag caattgacgt tggatacgta
     3901 tcaatttatt aattgtttca accatatgaa ctggaatacg gatcgtacgt gcttggtcag
     3961 cgattgctcg agtgatggct tgacgaatcc accatgtagc ataggttgaa aacttaaatc
     4021 ctttgcggta gtcaaatttt tcaactgcct tcatcagtcc catgtttcct tcttggatca
     4081 agtcaaggaa ctgcatgcct cgtcctacat aacgtttagc gatacttact actaaacgaa
     4141 ggtttgcttc tgccagacgc tgtttcgctt cttggtcgcc ttcttcgatt ttcaatgcta
     4201 acgctacttc ttcttccgca gttaaaaggg agacacggcc aatttctttc agatacatac
     4261 gaaccgggtc attgattttt acaccggttg gcgcagatag atcttctgtt tttgcttgtt
     4321 cagctttttt ctcagcactc ttcaagctgt gaattgatgg atcaccattt tcatccacca
     4381 cactgatacc tgcgtcttca atcttttgga tcaatttgtc catttcatct gcattcaaag
     4441 tgaatggtgt agcaagctga ttggataaat catcatatac aacttgtcgt ttgggtttgt
     4501 tttctttgat gaaagctgct actgctgctt catacgtttt tttatctgtt ccatttgcca
     4561 taaaagaagg ccccccttga gtagctattt ggcttgtttt aactgtttcg ttaaattgat
     4621 gatttcaatc gtcaattcaa gctcaagctg tttgtttcca gttcgtctgg cttcttgctg
     4681 catttgtttt tttaatgtta tttcatcagc taagccagat ttgctgatca catctagcaa
     4741 gtcttggatt tctcgtggcg aactttcttc tgccaccggt aaatattcga tttccaccac
     4801 caaccgcctt aaattttcat cttgcaaaaa gtctaaaaac tctgcaaggt tgaattcata
     4861 atgttgtgag acatatgcat cgaataaaaa ataaacttct gcatatgcat catgaataaa
     4921 ctgaaattct cgttctttta aaatattacg gacagaaact tctttaaatg cacgatataa
     4981 tagaatctgc tctgcctttt ctacttgcgt caaaggtctc tttttccgtg cagtttcttg
     5041 tctcgtaacc tgagtgtgtt gactttcctg aggttcttga cgattttgct gatttgtcct
     5101 tcgaagatcg cgcagttgtt tttgtatcgt ctctctgccg atctgaaact ctgttgacag
     5161 ttgagataga tacatatctt gttcgattgg tgaaacaact cggctaagtt caactagtaa
     5221 ttcttcgagg tattcgatct tgtctttttc attatctaaa tttttccctt gttttaaata
     5281 acgtgattta aaagtaaaga ctgtttcgcg cccatgagcc actaattctt ggaaagatgc
     5341 ttctccatac ttacggagat attcatcagg atcaagcttt tctggaacga ctacgacaga
     5401 aagtgctaag cgactgtttt gccgaagcaa ttcaatccct cgattggttg cttcaaaacc
     5461 tgcgttgtct ccgtcatagc aaaagaccaa ttctttcgtc agcttttcca tttgttggat
     5521 ctgttgagct gttagacttg tccccataga agctaatcca gttttgatac cagcctgcca
     5581 tgcagcaatc acatccataa acccttcgaa caacaaaatt tctccgtttt tccggatatc
     5641 gcttcttgct ttatcaagat taaataaaac ttgtctttta ttgaacagtt ctgtttctgg
     5701 actattcaag tatttgggct gatctttttc ctgccctttt tcaggtgcaa gccagcgtcc
     5761 tgaaaaaccg atcgtctggc cttgagaatt acgaagtgga aacatcactc tttgataaaa
     5821 acgatcagac atacggccat catcatgcgt gacaaacaat ccagagcgtt ctaacaattc
     5881 atttgaaatt gattcattct tgaatacttg ctgaagaaaa gaacgctcat taggtgcaaa
     5941 acctatgttg aattcttcaa ttaattctaa agacaaccca cgactttgca aatattctaa
     6001 agctggctgt ccagcttttg tatgcaatag catgtgatga tagatttccg ctgctttttc
     6061 atgagcagca atcagtttcc ccgtagcaga ttcaggattt tggacagcta tagtacgctg
     6121 acgccattga tcttctaacg gtatctgttc gaagtctgcc acttttacca ctgcttctgg
     6181 aaaacttaga ccttctagtt cttgaagaaa agtaaacacg tttccgccct taccacaacc
     6241 aaagcaatga aaaatctgct tgtcttctgc aacagaaaaa gacggtgtac gttcttcatg
     6301 aaaaggacac agccccaagt aatttttacc agactttttc aactggacat attgtccaat
     6361 cacctcaacg atattcgttt gactgcgtac tgtttcgata acctcttgag gaatccgttg
     6421 agccattttt ccaccccctg ctctattgat atcttttggg tttaacagaa gatatttcag
     6481 gagaataacc ggtaaaaaat cgcactaact gaaatcagtg cgattttaaa ggacacaatt
     6541 atacatgttt gattgtatca tatttcattg ctttttcaag tataacacat ttctatctca
     6601 ttaagcatca atttgctact cttttcagta actgattttg aatatcagcc ttccagtaca
     6661 atactcgaag aacaatattt acgagcatga atacaaagaa gtatcctacg ctctctccca
     6721 accctaagtt tacttccaaa attccatgga acagcattgc agctacatat atccctacga
     6781 tcgtcgttac tgctaggaag atgcgcaatg ggttgaaggg ataacaagct tttacaactg
     6841 ccatgcaact gatcccaatc agcaagtaat acatcaaagt agtagtttca gcatttcccc
     6901 atgcgaatgt ttttccaagt agatacaccg caatgatatt cactactaca agcaaggcat
     6961 ttggtaaagc attaataagt gctgtaggca aataacggta agtgattttt cttttatctt
     7021 gttcaaaaga taaaaagaag gaaggatacc cttcaatcgc taaatcaatc agcgtgattt
     7081 ggatagggat aaacggaaat ccaattgctg taacggcaca tataatcgat aaaataaatg
     7141 aataaatcgt tttgataaaa aagatgcctg agactttcgt cacattgttg accactcggc
     7201 gtccttcaaa caggacttct ggcaatgtag taaaatcaga gtccaacaat accagattcg
     7261 caatctgacg tgttgctcca tcaccttctg ccatagcaat gctacaatct gcttctctta
     7321 acgccaaaac atcattcaca ccgtcgcctg tcattgcaac cgttctgccg ctttctttca
     7381 attcatttac cagcaatttc ttttgctgag gactcactcg tccaaaaacc gtatagcgat
     7441 gagcagcttc tcgcacttcc atttcttcag taatttgaga aagatcgaca tacgcatcat
     7501 aattcggtaa tcctgcacgt ctagcgatat tggagacagt aaaaggattg tctccagaaa
     7561 tcactttcag atccacacct tcctcatgaa gataagctag tgtttcttga gcgttttttc
     7621 ggattggatc atcgatttca aacagagcaa ccggctcaac aaatgctggt ttttcttcgt
     7681 tcaattgctg atttggagcg atccctaaca taagtacgcg atagccattc gcttgggctg
     7741 tttttagact gtccggaagc cgatcttctt ccaccaaacg ttcaggcgct cctacgtaca
     7801 cgactcccag ttctggaaat tccatcgcgc cccatttgcg atcagaagaa aatgcgactt
     7861 tgtttgtcac atcataccga ttagaaagct caaaataatc tctcatagcc cgcatagtga
     7921 tgttgttatc actgctttct gccagatacg tccccataat ttctgggatg gctttctcgt
     7981 acagcggatg tagtgcttct acccgctgga tactcatttt tccttcagta atggtccctg
     8041 ttttatccaa gcataatgta tcgacatggg ccagcgtttc aattgaatac atgtcttgaa
     8101 caaggattcg tttttttgcc agtttggtta cccctgtcgt taatgcaata cttattagca
     8161 ataccaaacc ctttggaagc atgcctaaca aagctgctgc agatgcaata acagaaattt
     8221 tgatagaagc atcgcggatg aacagtgctt ccaaaaatag tatgatcccc aaaggaataa
     8281 tcacgaaact agtgaatttg gatactttgc gaatcgaatc tacaagttca gaacgaatag
     8341 ctttgtgagt ctttgcctcg ctagtcaagc ggacagcata attatctgca cctactcgga
     8401 taacttcagc catgacagaa ccgcttgtca aaaagcttcc agaaagcaat tcagctccag
     8461 tttctttgac aatcaaatca gattcgcctg tcaaaagagc ttcatttgct tctgcccgtc
     8521 cagatataac ctgtaaatcg gctggtatct gttctcctgc tccgattttt accaaatctc
     8581 ctaataccag ctctttagta gaaatcgatt gttctttacc gtcccggatc actagtactt
     8641 ctgattggga aacaatcgat aatttccgta caagatttct tgcatgtatt tcttgataaa
     8701 ttccaataac gatattgacg acaataattg ctaaatacgc catgttggaa tacgcaccta
     8761 caaataacag acaaatccca ataccaaagt tcagaaaatt gaataacgtc aatatattat
     8821 cgctgaatat ttgttttgta ctttttgatg catcttcctg gtagtcattt tgctggccag
     8881 aagccatttt ctgaactacc tcttgctgtg taagtccatt cattttcttt ttctccattt
     8941 tccgccaact ttcaaaaata atcacgtgtt taagtctagc gtgttccaag attaaaaaca
     9001 acttgttttt ataaaattta tagaaaaaca cacgaatgat tttacaaaaa agatgccaac
     9061 ctttttgttt gaatcacccg tgtgctgttt ttattatcaa tctatcttta cttatattgt
     9121 tcctccaaac ggctcatcca ccaacctagt gctgtaccgg ctataaaaag aacaggacaa
     9181 gctagtaccc cagcaactcc caatcccgcg taatcggtag gaatgatcat aatcgttgaa
     9241 gcaaaaacta cacctaaaat gaaatggaat agcttgctat atgcctttga gaaaatgtaa
     9301 tccatgattt ttgacaatcc aagaacacaa agaatcccac caattccgat cggcacaatc
     9361 acttcaagtc gtacttcttt aaatccgtca gccatcgctt tatacaatcc catatacacc
     9421 aaaaaattag aagggctcaa tcctgggacg atcataccca aaccaatcaa tccgccggca
     9481 atcagccatg ttccaaaatt ctgatcgact tgcccgtcaa ataattgagc gccgtataat
     9541 aaaaacaaat aagccagaac aaaagtaaca accatgatga tcacatcaga agtatctctt
     9601 ccttttttcc ctgcttcttt ccataacgca ggaacagttc cagcgataca tccgacaaaa
     9661 aaccataata tcgttgtttc atacgcacct aataaaaaac tgactaaaaa agaaagtaga
     9721 aagatgcctg tcactccgcc tagaccaaca ggcagaaaaa acaaaacatt ttctttgaaa
     9781 ttttttgtaa ggtgggctaa aaaagaaatc agccgctcat acatgccaaa gacagctgct
     9841 aatgcaccac cgctgactcc tggcaagata aatcctgaac cgataaacat tcccttcaca
     9901 aaccgtaaca tccagtcttt ggttgtgtac cccacttttt gattttccat tttttgcctt
     9961 ttttccacta tttgcgcact ccttctttca tttaatttta ttcatgattg aacatttcct
    10021 gctattttac ttaaaaactt tttcataggt ctctattatt ttttcattca ttttgacaat
    10081 atcaggataa aacatgttac ctccattatg atagagatag ccgccattgt aaagcaagga
    10141 ctggatccgc caatatcggt attgtttccc ttcctcattt cctaaaagtg ggctcagcac
    10201 atctttagaa tacgcttctg ctaattctac tgtgttttct ccgccatgat tttttacata
    10261 ggcaatataa tctttcccaa aattatatgc ttggacagct gtccataaat cgcatccttg
    10321 tttctttgct tctactatca tttccgccaa atatttgaca ccttgatcaa tactgtcagc
    10381 tggattatca atgatctcag cttctccatg cagactttcc gagctccgca tgagatctgt
    10441 cccttttcct tttgattcgg tcaatattat cgccttgatc aaattctcgt agttttccat
    10501 atgataattt tttgcagctg ttgttatttc tatttgatag ctttccactc gttttaccga
    10561 acgataagtt cttcctccta aaaacaacac cgcaccaata accgtcagaa tgatcatgta
    10621 aaaaagaata cggataatag ataatcgttt tcgcttcatt tcatcaccta gttattttga
    10681 catctttttt tatcttacta tttctttatc ttcttggcta tttcttcctt gatttctgtg
    10741 tacgccttaa gaaaaaatga attttcctgt attttttgtc atcttacgaa aatgatcgtt
    10801 gtttttcagt aaaaacaatt gcccaatgac tgatataatc gttatacttt acacagtagg
    10861 tgattatgtg aaaaaagaaa agaaatatgc aaaaatgcca gatgggagcg aaatttacta
    10921 tgaaaagagc ggtcaaggct tccctctctt tttactgcat ggcaatgacg gcagcggccg
    10981 ctttttttca gaacaggtcc ctgtactcga acgctactat accgtgtatc tagtagatag
    11041 ccgaggacat ggtcgttcga caaacgaagc atctatgttg aattttcagt taatggccga
    11101 ggatctgaat acgatcatgt tgctggaaaa aatcgatcag gcagattttc ttggatttag
    11161 tgatggagca aatctagcac tggtatttgc cagcagcttc cctaaaaaag ttcatcgatt
    11221 gattttaaat tctggaaaca cgcttgttaa aggcgttcgt ttttctgcca gagtcatcag
    11281 taatattcat tatgcatggg tatggttact ctctttattt cgcccaagtc ttcgcaaaaa
    11341 tcttttagtt atcaaacttt tgctgcatga catcgggctt acagaaaatg atttaaaaaa
    11401 aatcaactcc cccactttga tcatcgtggg aaaaaaagat gtaatcaagc taaaacattc
    11461 tttgtatata gctaaaacta taccaaaagc ttcctttgtg cttgtgaaag aacaaggaca
    11521 tgaattagct agaaaagatc ctgaacgctt caatcgagaa gttcttcaat ttctttctga
    11581 aacataacta ttaggaatag aaggtgattt atttgttaaa aaataccata caatggctga
    11641 aaaaccacct aggactgttc aaaacaatct ttttgatttc tgtcattgtc attattgtcg
    11701 gtgaattgat gtccatcggt aaaactctat ctatccagca attaggagaa acgtttgcca
    11761 ctatacctgt atggaaaagc ggacttatgc tgttgattgg tcttgtctct gtgttaccta
    11821 tgattggtta tgatattatt ttgaatcgga tgcttgaaca aaagcagaat cctcgttact
    11881 tgtttgagac aagctggctg atcaatacaa tcaacaatat tgccggtttc ggcgggtttg
    11941 ttagtgtggg gcttcgctct gaactatatg gacaaaagaa agagagcaga aacgtgatcc
    12001 aagcattatc gaaagttttc ttgtttttga tggctggact ttctatttac agtctgcttt
    12061 cttttttact tgtagtcttc acgccagttg ctcctttttt gaagcaatat tggatatggt
    12121 tgatcggcgg cggtctatat tttcctgtcg tctttttatt tactactttc aaaaagaaag
    12181 gattccttgg tggtacctca cttaaaactc aaggacagct attgctagtt tcattgttgg
    12241 aatggaccgg tgtcctgttt agtttccttt ctgttggtta tttaatggaa atccggattg
    12301 atctgtggca aacaattccg ttattcattg ctgcttcagt gataggaatc gtgtctatga
    12361 ttccaggaga aattgggagt ttcgatgtaa tgatgattat cgggttgtcc gctattggtg
    12421 tgccaagaga aatagtcgtt gtgtggatct tgctctatcg cctgttctac tacatcattc
    12481 cgtttttgat tggaatcgtc tttttcttca aaaatatcgg ttccactttt gatcagcgtt
    12541 attctggtat ccctaaacaa ttagcaacag aaattgcgca taaaatcgtg gttgtcttat
    12601 tatacttttc tggaatcatg ctagtacttt ctgccactat cccgcaagct tttacagagt
    12661 ttcgctggct tcatagtttg aacccattga aatttcattt tattatccaa tttcctagta
    12721 tactattggg atttctttta atcattatgg gcagagggat cgcagcacgt gtgaaacgag
    12781 catatttgcc tacaatcttc ttgattgttc tagctttact ttatgtttta ttaagtgatt
    12841 ttagtttcac accggtggtt ttcttgtcta ttctgcttct gatcattctt gcttctaaaa
    12901 acgaattatt tcgggaacaa ttgatctatt catgggagtg gcgtacgatc gatggaatca
    12961 ttattggtgc actgtccttg ctttacatcg ttattggggt atacaatctg ccggatttcc
    13021 ctcatcggcg tcaccatttt atttcctttt tcttatttcc ttctgaaaag atttggtttt
    13081 cgggattgtt ggccatcatc gctgttagtt tcatgatcgt tctatttgtt catttccttc
    13141 aaggagaaaa gaaacagatt ggcgaagcct ttaatgaaga aaaggccctg aagatcctta
    13201 caacttatgg aggaaactcc gatagtcaat tgatcttctt aaaagataag cggatgtttg
    13261 cctatgaaaa agacggtgaa ccaactgttc ttctccaatt cgcttgcttc aataataaat
    13321 gtattgtaat gggtgatcct tctggtaaga aagaagattt tccagaagca atcgaagctt
    13381 ttatcgaaga aacagatcga ctatgctatt taccagtttt ctacgaaaca agcgaagaga
    13441 ttgttatgat tttgcatgaa ttcggctacg atttcattaa gatgggggaa gaagcttatg
    13501 tagacctgaa tagttttaca acttcaggga aaaaaatgaa gggcactcgt gctgtcttga
    13561 atcggatcga acgagaagga tttacatttg atgtcttaca accaccattt tctactgagc
    13621 aaatgagtac tttcaaaaat atctctgata attggttggg ttcacgaaag gaaaaagggt
    13681 tttctttagg ctttttctct gaagattatc tacaaagagc gcctattgca gttgtcaaaa
    13741 atacccaaga agaaattgtc gcttttgcaa cgatcatgcc tacttacacg aacaataaag
    13801 taggaacgat tgatctgatg cgatatgatc ctaaaaaagc accttctggc agtatggatt
    13861 tcttgttctt gcatttgttt gcctatatga aagaagaaca tatccaatgg ttcaatttag
    13921 ggatggcccc tctttcaaat gtcggaactt ccagaaagag ttttttacag gaaaggattg
    13981 catacttggt ttacgagttt ggttctcatt tttattcttt ccatggatta agagaatata
    14041 aaagcaagta cgctacaaac tgggtctcac gttatactct atattctaga gatagttgga
    14101 ttgcatatgt tatgattgct ttgctgatta ttgataatgc gccagttgag cagacacaag
    14161 gtagaataag tggtttgaaa aaatggatac gaagaaagta ttaaaaactg cataccgata
    14221 gatgaacact ctcatgatga ataaaagaga ctgaagtaaa acgaaagttc atgcttctac
    14281 aatctcttct tatacaattg aagaacaaag agcggtcc
//
LOCUS       DAKBVG010000057        14226 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000057 DAKBVG010000000
VERSION     DAKBVG010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14226
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.057"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            63..581
                     /locus_tag="OUD09_001198"
     CDS             63..581
                     /locus_tag="OUD09_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9692478.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            562..954
                     /locus_tag="OUD09_001199"
     CDS             562..954
                     /locus_tag="OUD09_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HCT9692479.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            1030..1965
                     /locus_tag="OUD09_001200"
     CDS             1030..1965
                     /locus_tag="OUD09_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HCT9692480.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            2098..2877
                     /locus_tag="OUD09_001201"
     CDS             2098..2877
                     /locus_tag="OUD09_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HCT9692481.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            2870..3187
                     /locus_tag="OUD09_001202"
     CDS             2870..3187
                     /locus_tag="OUD09_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HCT9692482.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            3431..5041
                     /locus_tag="OUD09_001203"
     CDS             3431..5041
                     /locus_tag="OUD09_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCT9692483.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            5198..6046
                     /locus_tag="OUD09_001204"
     CDS             5198..6046
                     /locus_tag="OUD09_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HCT9692484.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            6277..6756
                     /locus_tag="OUD09_001205"
     CDS             6277..6756
                     /locus_tag="OUD09_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9692485.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            6789..9017
                     /locus_tag="OUD09_001206"
     CDS             6789..9017
                     /locus_tag="OUD09_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCT9692486.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            9146..10789
                     /locus_tag="OUD09_001207"
     CDS             9146..10789
                     /locus_tag="OUD09_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCT9692487.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(10862..11407)
                     /locus_tag="OUD09_001208"
     CDS             complement(10862..11407)
                     /locus_tag="OUD09_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HCT9692488.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(11430..11915)
                     /locus_tag="OUD09_001209"
     CDS             complement(11430..11915)
                     /locus_tag="OUD09_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCT9692489.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(11905..12717)
                     /locus_tag="OUD09_001210"
     CDS             complement(11905..12717)
                     /locus_tag="OUD09_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HCT9692490.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(12856..13968)
                     /locus_tag="OUD09_001211"
                     /pseudo
     CDS             complement(12856..13968)
                     /locus_tag="OUD09_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
     gene            complement(13989..>14226)
                     /locus_tag="OUD09_001212"
     CDS             complement(13989..>14226)
                     /locus_tag="OUD09_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HCT9692491.1"
                     /translation="PRKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNSRSVK
                     GLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         4559 a         2587 c         2867 g         4213 t
ORIGIN      
        1 cttttctgta tgtactaaat ggacgtgaat aacttgctga tgaaggaggg atttcattag
       61 gaatgtccac aaataaaata gcacagctta gcttattgtc tgctgcttgt gtagcaggta
      121 ggatcgcttt tcaatttatt ccgaatttcc agccaatgac tgctattttt ttatttatag
      181 tactttactt aagcttaaaa gatgcgttaa ttgtcatgag tctttctatt gcgatcagca
      241 gtttttattt tggtgttgga ccatgggtca tcggccaatg gatcagctat actgttgttc
      301 tgagtttgtt ttcaatcgtc tgcttgagaa aaacagtgca aaggaatcta tggttaaagg
      361 gagtctgttt ttttctggcg ggcatggttt acggaatagg tatgaccgtt tttgatacgc
      421 tgttatatcg tttgcctcaa ccttggatct attaccttca aggggtgtcg tttgatttga
      481 tgcacagtat aggcaatgtt gtgttctttc ttgtttttct gccagtcgtc aaacgttttt
      541 acaatcattc aggaggaaaa aatgaaaaaa ataatttata gtttgcttgc aatgagtgtc
      601 agtatactca ttttgacaag ttgcagtaca acctatacac aatcggcttc caaccaaaag
      661 gaggaagtaa aaacatcgca aaaagcaaca atcacgttac aagaaaatgg tcaaaacttt
      721 tcggaaaaag aagtgttctt caaaaaaggc gatgatttac tgagtatttt gaaacgaaat
      781 tttgaagtca aagaagataa cggcttcatc acttcgcttg aagggcactc acaggatgag
      841 aaaaatcaac tctattggat gtttactgtg gacgggaaaa gtgctactac tggtgccaaa
      901 gagatcaaat tgaatgatgg acaatcggtc attttcaatt tgtctaaact ataagtttca
      961 taaaaaactg ttaaagggcg aatattgcca taattcagtg agttcagttg atgaaagaag
     1021 gaaaataaca tggaaaattt taaaaaaagc ggaatgtttt ccgtaattgc tgctctaggg
     1081 gctaatattc tagttgctat cagcaaattt gtcggctatg caatcagtgg cagtgccgcc
     1141 atgctaaacg aaagcatcca cagtatcgtt gattgcagca atcagatttt gctgttattc
     1201 ggtgataaac aagccaataa aggacagagc gagttgcatc aatttggtga aggacgagca
     1261 aaatactttt tcagtacaat cgtcgcaatg atgcttttct tcggcggtgg ggcacttggg
     1321 gtgatggaag cttttgaaaa gttgacgcat ccatcccatg aagtgggtaa tccgatcatt
     1381 gttatagcaa tactgttatt tggtttggta atcgaaggaa gttcgttacg cgtggcaatg
     1441 aaagaaatca aagaattgaa tactgaaaaa ctttccacga tgcgcttttt gcgcgaaagt
     1501 cgacacagtg aaatactgat tatttttacg gaagacttgt gtgctgtaat cggtttggtc
     1561 ttggctttag caggaacaat attgacttat gcaaccggga ttgctgcatt tgatgcgatc
     1621 agcggattgc tgatcggttt cttgctgatg ggagccgcca ttttcctagc aaaagaattt
     1681 tatagtctga tcatcggtga aagcgtgaca gcaagcgatt tatcaaaaat caagttagct
     1741 tttgaacgtc ctgagatcag tcggctgatc gatataaaaa cagttcattt aagtccaacg
     1801 gagattttag ttgcagcaaa agcagatatc gttggtaaca aagagaatga agcttactcg
     1861 gtcatcaatg atattgaaaa aaatatgcgt cagtctcttc ctgacaagaa gatgtatata
     1921 tacattgaaa cagataaata tgacccaaat tattctcgta aataaaacgt ttattcatga
     1981 attgaagcaa aaatgagaaa aatataatct tgtttttaaa taaaacggct acaatttagc
     2041 gaatttatgt tacaatgagt ccgctgttta tttgttgtaa ttaaaggaga tattttaatg
     2101 aacaaaaaga aaagtgcgcc atggcttcaa gctttgcatg cagtgatgcc actttgtata
     2161 agctatatac cagtaggatt agcttgcgga gtattgttgc aaaaagttgg atttgatcct
     2221 ctcttatcag gactgttatc gattctggtc ttttccggtg gcgcgcagtt tttagttgca
     2281 tctatgctta cgacgcaagc atcatttgca acgacattat tgatggtttt ctttttagaa
     2341 ttacgctata cattattagg agcaagtctt gccggtttta tgaaaaaaga aaagcggacg
     2401 tttttggctg tcttttctca atcactgaat gatgaaaact atgcggtgaa ctacttaaag
     2461 ttttcaactg acccttcctg gaataaaaga aaggctttat atgtcaattg gttctcaatg
     2521 actgcatggg ccggtagcaa tatgatcggt aattttttcg gctcggtgat tcgagtagat
     2581 gctgaccttg tccattttgc tttgacggcc atgttcattt tcatgtttat catgcagatg
     2641 aaaagcgcac tactgatctt tacaggttta ttctcaggcg tattaggggt cgtattcatg
     2701 gtattattcc aaaatacttt cggattgatt gcagcaacag tcattgcttc atttgccgga
     2761 tttatgctgg agaaggcatt caaaaaagaa aaagctaaga aactaagaga ccgcggtaaa
     2821 gatgtcaatg agccgctctt caacttcccg gaacaggagg ccaatcacca tgactaaact
     2881 atatttgcta cttttagttg cttgtctgtt catcgtggca tatatcccgc gcttgttccc
     2941 gatgctttat tttactcatc gaaaagtgcc atcctggttc agtgactgga tgaaatatgt
     3001 accggtggca ttatttgcgg cgttagcttt taaagatgtt ttcattactc atgaacatct
     3061 ggatattgct tggaatatca aaatagctgc aatgattcta gtagcaggcg tggcttacaa
     3121 aacacgttcg atggcgttgt cagttttgac aggacttgcg tctgtctttt tgctgtctat
     3181 gctttaaact gttttatttt attaaaaaca gagattgcga cataagttgt tcaaactgat
     3241 ctatccgaac tgcatttgca acagtcaatt ctggatgcaa gtttattcgt agggataaaa
     3301 aagattagtg ttggcttatg atgcaagctc ttttttattt taggaattag agagggattt
     3361 gtccttctat gattttttgc tataataggg aagatgttta ttttatgaaa gaatagttga
     3421 gggaatttaa atgaataaaa aattgatgca aggaacattt tggctaacct ttgctaatct
     3481 tctatgtaaa gttttagggg tagtctattt gattccctgg ctaagtatga tggggaacaa
     3541 tcaagatggt atgcttgcta cgacattgta taatgttggc tatcttcctt atggattgtt
     3601 cctgatgctt ggtacagtcg gatttccaaa tgcgattgca aaaaaagtag ctgtagccac
     3661 aaaagaagga gataacgctg cttgtcggct gatttttaga agtacgatca atatcatgtt
     3721 tgtcatcggt atcgcctctg ctgcactgat gtatttattt gctcctttac tagccggaat
     3781 cagtccgatt gctaacgtta ataatgggat actagctatc agaagtttat gcccatcttt
     3841 gattgctatc ccaattctta gtgcaatgag aggatatttc caaggaaaaa atgatctgcg
     3901 gccttatgga acttctttga ttatcgaaca ggctgtgcga gtgatcgtta tcttggctgg
     3961 tacttattac ttgcgcgtat tgactgacgg aaccatatta gaagctgtat tgatcagtac
     4021 agtcgcttct ttcttcggtg ggttagctgc gattatccat atgtatatcg tcggacgcag
     4081 aaaagacttc ttcgaactta aagatttttt gatttccagc cgttatttcg aaagagaaaa
     4141 ccgatctgcc tctgtctcta tcattcggga aacactaccg tttatttttg tcggctcagt
     4201 cattactatc gttcaaatga tcgaccaagt aacgatgaaa ccactgcttc actttttccg
     4261 tccagagatc gcagatcagc aactggaatt cttattcagc cgtgcttctg tcaatccaaa
     4321 taaattgaca ttgatcctga tctccatggt aggaacagta gctataagca gcttgccaat
     4381 attaagcacg atgaagaaaa aagatcggat acaaatcgaa aaaacagtag gagatagttt
     4441 ttcgattgcg ttattgattc ttcttccttc attgactgga atgtctttat tagctggacc
     4501 actatacaca ttgttttttg gctatgatcc tgaaagtgtc ggatatttcc aaatggcttt
     4561 gctagcttct ttgtttttct ctttattcac gatcctatct acgatgcttc aatctttgaa
     4621 tcaccatcgt tttgcgatcc gattaactgt agaagcaatt atcctgaaag tcatcttcca
     4681 agtaatcgga ttaggattag ttggcggata cgggatgagc ttatcaaatg gcttagcatt
     4741 cggtattgtc tttgttcgcg gctatcactt tatgtgcaaa gaataccgaa tagcaccttt
     4801 agcaaagatc agcaattttt tcttaaaaac tttccgctcc accctgatta tgcttgcagt
     4861 atgttttgcc gttttctttt tgctgaatca gcgattgtca atggaatcaa aaagctatgc
     4921 aatgatttat tgtgtggctg taggaagtat tggcggctta gtatttttat tttctcaatt
     4981 cggaaaaaat gggtttcgaa tgttgaaaaa tgttaaacat catagcagaa gcaaagaata
     5041 ggttaactaa tcgttttttg ctgtcacttg aaaaacggca actcgtatga ttacgcttgt
     5101 cgtttttcta ttttccaatt ttagtttcat atctaatctt acttatgata tagttataag
     5161 gtctggaaaa attctttgaa acgaggaaat taaacacatg aaacttgcag tagttacaga
     5221 tagttcggca tatctgccgg aacgtatcaa gaaccatccc gacttattcg ttattccaat
     5281 ccctttgatt atggatggga aaatttataa cgaaggaatc gatatcgaag cggatgaata
     5341 ttatggactt ttaaaaaaca gtaaagattt tcctactact tcacaacctg tcgtagggga
     5401 agttttgggt ttatatgagg aattgaaaga gaaagggtat gacacgattt taagtatcca
     5461 tctttcttca gggatttctg gctttgtcaa cacattattt gcgatgaaag atgacatcag
     5521 cggagtaacg gtcattcctt atgactctaa gatcactagt atgccgatgg gacacatggt
     5581 agaagctgcc ttagacttaa atgatgaagg tcagtccctt gatgaaatac ttgcacatat
     5641 cgatcgaatc cgagataata cctacgctta tctgattgta gatgatttga acaatttagt
     5701 acgaggagga cgtttgacaa atggtgcagc gctgattggc agcttattaa aaatcaaacc
     5761 gatcctgaca ttttctgaag ggaaaattgt tttattcgaa aaaatccgtt caagtaaaaa
     5821 agcatttgct cgagctgaag aaattattgg tagaagaaat aacgaaatac aaccgccagt
     5881 caaactttac gtgatacatg caaataattt agaagtagcg attgcggaga aagagaaatt
     5941 gcagaaaaaa tatccaaatg cagaaatcga aattggtcat ttcggaccag tcattggtac
     6001 acatttaggt gaaaaagcca tcggcattgc catttcagcg caataattcc ttttattcaa
     6061 aaaacatacc aaaaatattc caacatcatc ttggactttc tacatgttac taatgagcgt
     6121 gtaaaagaac gagatggtgt ttttatttct atcaatgaag ctctttttct ttgcaatcct
     6181 cacttcaatc ctctataatg tcgcatatag tttagtaaat gagtaaacta ctaatttact
     6241 tatttactaa aaataaaagg atgaggagtt aaaataatgg aaataccaac aaatttaaaa
     6301 cataaaccag taatcgttgt agaagactat gataaagttg atggaagaaa tgcattaaat
     6361 acggatgcaa aagggctttc actgggcctt gctcagtgga atgatcgtgg aaaagtcgat
     6421 atctcaggca aggtatggcg acatactgga gaaaaatggt cacgacagtc ggaagaatta
     6481 ccaatcacac gtattttaga tttagcgatc ttagctgcac aaggaagttt atacttccaa
     6541 gatgcttatc gttatgaaaa attttatgat ccagaaaatc cagtcgtaga catcattgga
     6601 ttacaaggga accgaatgac ggtagaagta gataccaaaa atccaaagat cgatgaagac
     6661 atcattttgt attacgatac actacaaaaa gacggagaac tgattgggga acgattccgt
     6721 attttaaaac gtcttttaga tgagctagga tattaataga cagagatgat caaaagggga
     6781 agagaagcat ggagaatttc tcactaacag aaaaccgaac cttttcacgg ctagaaaagc
     6841 aaatgatctg gaagaaaccg aaaagccatc ggacaagtgc agaagaatgg cggatcgcct
     6901 ccgaaatcaa agggaattgg caagatcctg aaatgaaaat cagtaacgta cttttggaag
     6961 gagatgccgg gtcggggaag acacaattag ccaaagcact atctgctgat ctgcaacttc
     7021 cctatacaaa agtgacctgt tttgcagata tggacaaatc agatgttttc ggagccttat
     7081 tacctgtagt agattctaat aaggaacaag accaagaatt actagaagct atttaccaaa
     7141 cagatacact tgaagctgta ctgacactga tccagaacca ttacggcatt gatccttttt
     7201 ctgctaaaga aaaattagca gagctagttc aaaggatcga agctgattat ccagatgcaa
     7261 tccattacag ctattaccct tctgaaatcg ttcgagcgat tgaaaaagga tatttattag
     7321 agattcaaga gccgactgtc atccgagatg cctctgttct tgtggcactt aactcagcgc
     7381 tcgaaccaaa tgggatgttg aatttaccga caggaatcat tcgtcgccat ccagattgcg
     7441 tcattgtgat cactacaaat cgaaattacc aaggaaatcg cccattaaat gaatctttaa
     7501 gagatcggat gcaacatgcg gaaaaaatgg atcttcctgc cttagaagtc atggcagaac
     7561 gtgcgatggc gaaaactatg atccaaaaac aagaacttct cttaaagatg gcagagatta
     7621 tccgtttgtt ggatgaaact gcaaaagcta acgccatcaa aggtgtagca ggaatgcgtt
     7681 cttattttta ttgggcaaat acctataagc aaggacaaga tttactccag tcgatttatc
     7741 caaaagtttt gtataaatta tccactgatt cagatgaatt ggcaattctt gaacaagctt
     7801 tgtctgacag tggattgcta ctggaacttc aggacatatt gagaaaagaa aaatggggtc
     7861 atcctcaatc tgagcataca aaaggaagaa ccattagcgc cgaagaagca aatgaacgaa
     7921 acatcactga aaatgctgaa gaaattctgc gcttagatga aaagaaatcg ttgtctgctg
     7981 aagaaccaaa tgagggagaa gagaaacaag aagaaacaat agaaaaaaac gagattcctc
     8041 ctcaaatgca aaaagaacgc agtagcgata tcgaaggaaa aaatcaaaaa gaacaacaaa
     8101 caggagatga ttctcaagaa atgtctgctt ctgacatgga agcacatgat aaggaaatca
     8161 agaagcaact gaataaagaa gcgcgacaaa tcatgaagga aacgattcat gaaaaagaag
     8221 gtctcatcgt tcaccgacca gctttccaag gaaaaacgcc ggaagcacgt gagctacaca
     8281 tgcaagtaat gccaatagta gaatcactta gccggcaaat tttagagtta ctggataatg
     8341 aacaaacaga gacgtaccaa aaaggaaaat acgaaggtca gcggttcaat gcaagcaaag
     8401 tagcatatgg tgatttgaga aattttgaca aaaagaaccc gccacatgag caaccttctt
     8461 tagcggtagc tgtacggatc gatgaatctg ggtcaatgat acgggatgat cggatcgaag
     8521 cggcaaaaaa agcagcgatt gccattgccg aattttctaa gaaagtcgat atcccattgt
     8581 tgatttacgg agatacagca gaccgttcgt caagagaaaa gacttctttg ttttcttata
     8641 aagaatttga agacggattt caatggttaa atgaaaaatt agtcactatg aaaccaaggc
     8701 aaaacaatcg tgatggtgct gcacttcgtt tagcagcaga aaaattaaac agacaatccg
     8761 caacaaccaa actgttgctt aatattagtg acggtcagcc aaaagcgttg ccggattata
     8821 caggcaaaaa agcaaaagaa gatatccagt ctgttttgaa agaatatgaa cgtcaaggaa
     8881 tcctatttgt ctccgcagct attggccaag acaaagaaga aatcaagtcg atctacggtg
     8941 aaagtcgttt tgtagatatt actgacttga atgaatttcc caagcaaatg attcaactga
     9001 ttgcccgcta tctatgataa aataaagctc aacgaacgca cttagcagat tcgttgagct
     9061 tttcgtttct agttaccggt aacaaggata cttctaaaat agaatcggtt ataatcaaga
     9121 gggaccgtta aaggagtgaa acgatatgat acaagaacaa aaatggtgga aagaagcagt
     9181 aggctatcaa atctatcctg ctagtttcaa agatagcaat ggcgatggtc aaggagacat
     9241 caatgggatc cgtgaaaaac ttaattattt gaaagatctt ggtatcggct ttatctggat
     9301 caatccgatc tatcaatctc catttgtcga taatggttat gacgtaagtg actatcaaga
     9361 tatagaagag agattcggaa cgatggaaga ttttgattta ttgctaaaag aagcacatga
     9421 attagggatc aaagtgatta tggatcttgt catcaaccat agttcggatc agcatcaatg
     9481 gtttgaagaa tcgaaaaaat ctaaagaaag cccgtatcgc gattattaca tttgggtcga
     9541 cggggtagat ggcaaagaac cgaataattg gacatcgatt ttcggcggtt ctgcttggga
     9601 gtattctcat gaaactggac agtattatct gcatgttttt gcaaaagaac aaccagactt
     9661 aaattgggaa agcgagaaat tgaaagaaga actattcaat atgatccgat ggtggttgga
     9721 taaagggata gatggatttc gtttagatgc gatttctcat gtaaaaaaag atgaatattc
     9781 agtaaaagcc acagagaacc ctttctcacc ctttcaaaat gtctcaggaa tcgaagaaca
     9841 tttgaccgat ttgaaacatg tttttgaaga gtacgatatc atgactgtcg gagaagcaag
     9901 tggtgtcact gcagaagaag gaccacaatg ggtagggaaa gacggttatt tcgatatgat
     9961 attcgaattt gatcatatcc atatctggca gcaagaaaaa gaagggcaat tagatgtcct
    10021 taaattgaaa cttgcattaa gcgcttggca aacttcattg gatggtatcg gatggaacgc
    10081 tttatatatg gaaaaccatg atgttcctcg tgccgtttca gtgtttggag atactcgtcc
    10141 cgatttttgg gctatgtctg caaaagcaat cgctatgatg tatttctttt tgcaaggtac
    10201 gccatttatt tatcaaggac aggaaatcgg aatgacgaat atgccctttg aatcgatcga
    10261 tcaagttgat gcagtagatt cgaaaagact ttataaaagg ctgcttgcgg aaggtaagac
    10321 aagagaagaa gcattggata tcattcgtga aacaacgaga gacaacagca ggacacccat
    10381 gcaatggact tcggaacaat atgctggttt ttctacacat gaaccttggc tgatcactaa
    10441 tccaaatacc aaaacgatca atgtagaaca acaagaatat gaaccagagt cggtcctgca
    10501 attctataag aacatgatcc gcattagaca aaccaataaa ggtctgatat acggttctta
    10561 taaagaatat cttcatgaac atccacaact ttatgtctat gaaagatatt tagaagatga
    10621 acaatatctt attatggtaa acttaacaga atcacttgct gactatgaac ttccaaaaga
    10681 agcagatcag tcctggacat tactcttgtc aaattcctct agcggagaat ttgaagctaa
    10741 aggcatacta gcaccttatg aagctcggtt atataaaaca aacaagtaaa acttcctcgt
    10801 taaagttcat tagttggact ttgcgaggaa gtttttcaga ttgctctgtc aattactcat
    10861 ttcaataatt cgctctagga ccaccgatat atttaggacg agagcgaagg gctgtcacgt
    10921 gagcttgtcc aagggttttc aaacgaggtc tgacaggtac ttggacatgt ttgatatgca
    10981 taccgattga tgtgtcaccg atatcgatac cggcttgtcc aactacgtgt tccacttcga
    11041 caggatcatt gaactggtca aaagcagcaa cagaacaagc acctccagca tgtagtgcag
    11101 gaacaacaga gacaatttcg aaattttttg cttctgccac tgttcgttct actactaatg
    11161 ctcggttcaa atgttcacaa ccttggacag ctagcgcaat ttctttagga tccaacaact
    11221 cttttaacgt acggatgatc cattgaccaa cttccgcact ggaatttttt ccaatatgtc
    11281 ctccaacgac ttcgcttgtg ctgcatccta aaacaaaaag atcacctttt ttcaagtgtg
    11341 cctcagccaa tatgtcatta actatttcag tcaattggtc ttttaattct ttttctgaca
    11401 acttcatttt ctaaaacctt ctttttcaat taatattgct tccgtgcttg aatacgttga
    11461 agacttgcag ttataggaaa ggcaagtccc attccaatca gattttggaa gatgtttcct
    11521 ggtatagaag ccactccagc cggccaatca tataaaatcc aaccggcgat accatagccg
    11581 atgatcataa ctaagcttcc taataaaagc cctaatcccc acatacgagt agaagatttg
    11641 ctgaaagaac cagcaacata tccttgtaac ccgtggatca ctaatgaaaa aactgcccac
    11701 tgcggatatc ctgaaatcaa atcgatcaat agaccactgg aagcaccaac aactaatcca
    11761 cccgttggac cataaagaaa agcagcggta tagatcccgg cctcgcataa tgtcacaaat
    11821 ccattcgtag ccggtacagg gatcaaaatt gttaatgaaa gggcaactgt caacgcaatc
    11881 agcatagcat agatcgttaa ttgcttagtt ttcatttctc attcctcctt tttgaatacc
    11941 gccagaccag acacttccgg tatgatcgtt catcagcacg ccattagaaa tacaatgggc
    12001 aacaaatttt ttactttctg ctactgcttt ggtaaaatct gcccctaaca caagattcgc
    12061 tgttattgca gaagaaaagg cacaaccggc cccgtttact gttgtagtgt cgattttttt
    12121 atgagaaaat acctcataat gatcaattga atagagaaga tccacagctt catctccagc
    12181 catccttgtg ccgcctttga tcacaatgtt ttttgttcct gaagcagtga gttctttagc
    12241 gagatggatc atttctgtta acgaagaggg aagagcttga ccagaaagta aagcagcttc
    12301 tgccaaatta ggcgtaatca tcgtaacaag tggaaaaagt tcttcgatga gctgcgaaat
    12361 atacaattga ttgatcacag cttcagtttc tttgaatgcc aaaacagggt ctaaaatgat
    12421 tgggatattt tgttttgttt ttatgaagtc tcgaacaacc atgattgctt cacttgaatt
    12481 caataaccca attttgatac catctaatga agtcatttta tcaatcgaaa ctaactgtcg
    12541 tttaagcaat tccaaagata tagattccag cataaatcgc tcattaatat ctagtgcccc
    12601 tatagaagtc aaggcgctta gtccgaatag accaaaattc tcaaaagttt ttaaatctgt
    12661 ttggattcct ccaccagcaa atggatcaga accgccaatt gtcaagattc ttttcataac
    12721 attttcctcc atgaataaga gtataacgaa attttcaaaa aagaaaaaca gccaattggc
    12781 atatttttat ctcatccaat ttaaaaaaac aaaaagaaaa gaaagaccaa aatggatctt
    12841 tctttcctta aatagttagg acttttgtga ttttaattct ttctcgattt gaagccgttg
    12901 cactttcatt gtagctgttc gcgggattgc atcaaaatca cggatgatag ggacgttcaa
    12961 gcgaggaagt tcatggactt gctcccacca tcgattccaa tccatttctt tatcaggagc
    13021 taaagcaata atcggttgtg gagaatgatt tttatccctt acaatcacta cttctgccaa
    13081 aaaatctaac gaatctaata aaaagtcttc aatagctaat gtactattga tcgtttcgat
    13141 caaatccact tgccgatctt tcaaaaatag gtggccatgc ttatccataa aaccgtaatc
    13201 tccgctatcc caccaatcgc cataaacatt ttcctggaac cgtgcatctt ccttgtaata
    13261 agtcaaagcc cgtcctttag acagtaattg tatatgacca tctgtgtttt caggtaggac
    13321 attcccaact gaatctgtaa tccgagcgct tgtcatgtct tccagtccaa cgcccatatc
    13381 tcttgcatca cttgttttca aactctctag tgtatgagcc ttaagaatca ttggtccgca
    13441 ttcactttgg ccatatactt gaagaaatac cgcatcctgt gccatcgacg ttcgcaaaaa
    13501 tgtcttcatt gtctggttgt ttatagcatc aaaagtggag tgatagtatc ggatacccga
    13561 aaaagcttct ggatgctcct tagcagtaaa acgccactga acaaagttat tcggatgggt
    13621 ctctagtgca attggccggt gagcttccaa catatgaaca acttttgaac tttgtgcgtt
    13681 tgctaaaggc atcataggaa atcccatgga catcaaagaa gagataccaa tattgaatct
    13741 ggaatgcact ggagagatgt gaaaagcgat taatttcttt tccgaaattt ttgtgaaaac
    13801 tgttttttgc catttcgtcc gccatcccat agaatgagca gaatgacaaa taagtttagg
    13861 gataccagtt gtaccagaag tatgggtcat ataagaaatt tcatttttgt ctaaaaatga
    13921 ttgagaaaca gacaaagcag gttgttccat taaataaaga ataggaatac ccgtggtgct
    13981 agttaatttt aatatcctaa agaatatctc aaagttcctt caaaacgtcg ttgtgctcga
    14041 cttaatagaa cactgtaagc cagtaatatg ctttcaaatc tactctctag ccctttaaca
    14101 gaacgtgagt tgaaattttg acaacctaat ttttctaagg aagaaaaaac agtctcaact
    14161 gtttttcttt gttttccaag taagctgtgg tctagttttt ctgatttatc catgttttta
    14221 cgtggc
//
LOCUS       DAKBVG010000058        14189 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000058 DAKBVG010000000
VERSION     DAKBVG010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.058"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(56..880)
                     /locus_tag="OUD09_001213"
     CDS             complement(56..880)
                     /locus_tag="OUD09_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCT9692492.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(853..1533)
                     /locus_tag="OUD09_001214"
     CDS             complement(853..1533)
                     /locus_tag="OUD09_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HCT9692493.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(1556..2494)
                     /locus_tag="OUD09_001215"
     CDS             complement(1556..2494)
                     /locus_tag="OUD09_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HCT9692494.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(2739..3602)
                     /locus_tag="OUD09_001216"
     CDS             complement(2739..3602)
                     /locus_tag="OUD09_001216"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCT9692495.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(3744..6317)
                     /gene="mprF"
                     /locus_tag="OUD09_001217"
     CDS             complement(3744..6317)
                     /gene="mprF"
                     /locus_tag="OUD09_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HCT9692496.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLP
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            6458..7264
                     /gene="proC"
                     /locus_tag="OUD09_001218"
     CDS             6458..7264
                     /gene="proC"
                     /locus_tag="OUD09_001218"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCT9692497.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(7343..8899)
                     /gene="rny"
                     /locus_tag="OUD09_001219"
     CDS             complement(7343..8899)
                     /gene="rny"
                     /locus_tag="OUD09_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HCT9692498.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(9310..10359)
                     /gene="recA"
                     /locus_tag="OUD09_001220"
     CDS             complement(9310..10359)
                     /gene="recA"
                     /locus_tag="OUD09_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCT9692499.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(10468..11709)
                     /locus_tag="OUD09_001221"
     CDS             complement(10468..11709)
                     /locus_tag="OUD09_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HCT9692500.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(11949..12527)
                     /gene="pgsA"
                     /locus_tag="OUD09_001222"
     CDS             complement(11949..12527)
                     /gene="pgsA"
                     /locus_tag="OUD09_001222"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCT9692501.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(12736..13659)
                     /locus_tag="OUD09_001223"
     CDS             complement(12736..13659)
                     /locus_tag="OUD09_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCT9692502.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
BASE COUNT         4064 a         3157 c         2475 g         4493 t
ORIGIN      
        1 aaaaagaagc ctataaaaaa tttttcgttt ttataaggct tcttttcttt ataatttagt
       61 tattttttct tctgaaggca accaatattc gtttgatgat atttacaatc aggaacaaag
      121 caataaagac taatgtgatt gttgctcctg gaggtgtatc tagataaaac gagctagtta
      181 gtcctgacag catccctaga aatcctacac aaacactgat agcaatcacg acattgaagc
      241 ttttgcctaa tcgcataccg attgctgcgg gaagaaccat gattgcggat atcaaaagtg
      301 caccggcaat cgggatcatc acagcaatag ccacaccagt aatcacattg aacatcatcg
      361 acatcaaatg aacgggtagc ccatccacgt gggcggtatc ttcatcaaaa gtcagaacat
      421 acatcggccg tttgaataaa aagaacaata taccgatggc aacgaataag attcctaaaa
      481 agatgacttg ctcccatgta atggtcacga tcgaaccaaa cagataggat tggatacttg
      541 ttgctgaatt ccctccgctc aaattcatca atactaacgc tagcgccaaa ccaccagcca
      601 ttaaaatggc aatggagatt tctgaatagg ttcggtagat cgttcgcaga tactccaata
      661 tcacagctgc taatacaacg actagcatgg tcgtaagtgt aggattaagg ttcaaaaaga
      721 atcctaacgc tacccctgct aaagagacat gagaaagcgt atcagccatc agcgattgtc
      781 ttctgatcac taaaaacaca cctagcatcg gggcgattcc ggcaatgaag atagcggcaa
      841 gaaacgctct tctcatgaat tcatatgaaa gcaacgccat tgagaatcct cctttctcac
      901 cagtcggatc tgtctgtccg catactgttt gatatcttca tgatcatggg tgatcattag
      961 aactgatttc ccatggacat gcgcactatg tcggagaaga cgataaaatt catttctgga
     1021 ttcttcatcc atacctgttg ttggttcatc aagaacaaat aagtcagggt cagtagcaaa
     1081 aatacgcgct aaactgattc gctgcttttg tccgccagac aattcaccta ttcgccgatt
     1141 tcgcatatcc cacatcccta cagcttctaa agcacgctgt acatgttgat gatctttttc
     1201 agagagacgc ttgaaccagc ggcctcttgg atatcttcct gaacggacta attcgatcac
     1261 cgtactagga aaacctgcat tgaaagatgc aacttgttga ggaatataac cgatacttaa
     1321 tttttttcct tcttgatttt tctcagcaat ctttatcatt ccttttgaag gtttcagtaa
     1381 tcctaacgat gctttcacca aggttgactt ggctgctcca ttttctccag ttaaaatgac
     1441 aaattcaccc gcatctacat gataagagac atcttctagt acaggttcat catcatagta
     1501 aaaggtcaaa tcagatattt cgatataacg caccttacaa cctccattct aaaacttaat
     1561 tgatactttc ttttaaagct ttcagatttt tttccatgac agaaatataa ttttcgcctg
     1621 ctttgatttg ttcattagtc aagctttcta aaggattcaa cacttctagc ttcactcctg
     1681 tttcattcga caatgtctca gctaccttcg aagaggcatt ttcttcaaaa taaatcactt
     1741 tgatgttgtt tttcttcaca aattctttta gctcagctaa gcgacttggt gtgggttcct
     1801 gatctggtga aacgcccgaa atcgctactt gttttaagtc gtattgattc gctaaataag
     1861 caaaagcagc atgctgagtg acaaatgtgc ggtttgttgc atcttttagc tcatcggagt
     1921 atttttgatc cagtgcttca agctttttga tataagcatc ggcattcttc gtaaaagttt
     1981 cctttttatc tggatatttt tcgcctaatt cttcagcaat cgttctgact tcttttatag
     2041 ctaataccgg atcgagccaa acatgtggat cgtattcatg tgaatgacca tgcccttctt
     2101 ctgattcttc atgttcgtct gcttcttgcg attctaaacg gtcgattcct ttagctgctt
     2161 cgatcatcaa ggtctgtttg ctatccactg aatcctttaa cgaatccaca aaaaattcca
     2221 tgtctgaact attatacacg aaaacatctg catcggaaat ttctgctctt tcttttgcac
     2281 ttggttcgaa atcatgcggc tccgttccag cagggatcaa aagtttcacg tttccttcat
     2341 cccctacgat ctcttttgta aaatcataca tcggataaaa agtcgtaacg atcgtcaaat
     2401 cctctttttt gtctgcttcc ttgttcgtat ttccacatgc tgcaaacagt aaagcaccta
     2461 tcattaccgt agctgtcaaa agatattttt tcatttatat gctcctttca atgaacaaat
     2521 cggaatttta cgatttatta acgtgaacta atgtatcaaa ataaaaaaac acagtcaata
     2581 gaaatccatc aatttctgat ttaagcatgt atattcttga ttaaatcgta aaatggatga
     2641 gttgctgctc atatctagcc atttcactac aataaaaaac tgaagaatac cattttagga
     2701 ttcctcagtt tttcatcacc tattttactt cttatatttt attgcagggt tcgtactaaa
     2761 taaacttcct cacaaaaacc ttttaaccca ttatatactc tctgcgctct tgaataatgt
     2821 tgacacaagg caaaaacggt tggtccgctt ccactcatca atgcagcatc tgctccgtac
     2881 ttgatcatac gctctttgat ttgatggatg actggatgcc gctttcccgt tatatcttct
     2941 aaactgtttc ccattttttt gatcatctgc tgataatctt gctctcggat ggcttcagca
     3001 agtccgtgga tgtcttgatg ttggatggct tgcgtatcta cttctctaaa cactgtacgt
     3061 gtagagacac tcattcttgg tttgaccaac acaacccagc actgtggcat agaaggtaat
     3121 acttggattt cttccccttt gccaccgaca aatgccgtac ttccgtagac acagtaagga
     3181 acatctgtac cgacttccaa accgatttct gccagttctt ctaacgaaca gcccagattc
     3241 cataattggt tgatccctct tagagcagct gcacagtctg tacttcctcc gcctaatcca
     3301 gctgctactg gaatattctt cttaacaaag atcttgacac cggttttgat attgtattta
     3361 ttttttagca ataaagcagc ttgataaaca ttgttccgtt catctaccgg taaaaatgct
     3421 ttatttgttt ctacaatgat tttgtcttct gttatttctt caaaaaatag atgatctgcc
     3481 aagtctacgc tcgacatcac catttccaac tcatgatacc catcttgccg tctatataat
     3541 acatccagtc ctaaatttat cttggctggc gctttttcaa tcaattccat cctctcaccc
     3601 attcgaaaag tctaagtcgc aagtattcta tcatagggaa aaataaaagt atagcttatg
     3661 tctgcctagt ggttgatttt gtcatcgttt caacagcttt cacagaattt tctcttcata
     3721 atccaaacaa gttctagtga ttcttataat tctgtgagca cttcttctgc caaactcgga
     3781 acatttcctt tttcttttcc tatcagactg agtagctgca tggtagcaaa tccgaaatta
     3841 cttcctttcc tataagcaaa ataacgtcct tcccaatgat cgacatattt taatttcttt
     3901 tcataagcag actggaagga atagaaaaaa ttaccataat tatatacgat atgcatgact
     3961 ttttcttgaa agaaagaaaa atctgtttct ccaacattag ctaatggagc taagcctaga
     4021 ttggcttccg agtagcccat tttctcgtat acaataaaca gattggctaa cagatagtca
     4081 gctaattcct cagattcttt aggtaagacc cgcaacaaat catatcccgc ctgcttcttc
     4141 gtcatcggct gagcggtgat gaagccaacg actttttgat ctttacggac aataccgaca
     4201 ccgctagata atacatatga tttatcaaat cgtcccacag agaagtattt ttctcgtgca
     4261 ccttgaagcc atttttgtga aacttcattt acagcttgat gtaattcttc cggcagttga
     4321 tcgtaccaag taaaatggta tccttctttc atcaaacgtt gatattcaag ttgctgactc
     4381 agccgagaca agtcttctgt tgctagatca acaatccctg cttcacctac tttcgtgaag
     4441 tgaaaaccca attcatgaag catcactacg tagctttctg atatcttata aaacgcaagc
     4501 tgatagccta aacaatctgc ttgtttcata aactgattcg ttgctgcccg catcatttta
     4561 ggattgccaa ctggatctcc taaaacaaag catttgtttc ctttgatctg ataaccaaaa
     4621 acgacttgat cttggtcgtc ttcttggtaa taaaaataac tgtaacctga caagcgaagc
     4681 tgatggcttg tagctgtgcc tccgtatgtt tccaataaac ggtcaaaacg gtctttttcg
     4741 tactcactgc caagcttcgt agtatcttca gctaaatatt gataaagcgc cactaaagct
     4801 agcaatgaga tgcccaaacc aataagccct gagaaccaca catcatctga aggaaacaaa
     4861 ataaaatgat ttcctgttat tcgaccgact aaaaagcgtc cgctgtgata gcctgcaatt
     4921 gcgtaaacga taaacaaaaa gccgaacaag ctagcatcaa agattatagc tccccaagaa
     4981 taaacgaatt ttgttcgata aaattctttt ctggataacc acacagctaa cagaatcaag
     5041 acatatacca atatcaactg ccaagataac gtacgtgtga ttgtatttaa aataccaaaa
     5101 gatagtaaag caatcgtagg cagaaatgcc tttttgactt ttactgaaag tgctctagca
     5161 aggccgagta ataaaaagcc aaccaataga ttcactgtct gatctaaaaa attgaatgag
     5221 aacggcaata atacttgaaa tagccgactg attgcagaca gattagtgat tgttgataga
     5281 agtaccatca tgatccccgc aaaatagaca gcacttacga gcacgatatg cgcaaccttt
     5341 tggaaaaaca gtcgcggaat gttatctaaa aatcgattga ttttgactcc tgtatgtgtg
     5401 atgaacaata gaagtcccgt cataaaaggt agaacataat aaaacaaccg ataatataat
     5461 agccaaacta ctgctacatc ttgccgaaca ggcaattgac tcattcctaa gatcatcaaa
     5521 acgtcaaaag tccccattcc acctgggacc atcgtcagca ttcctatcaa tgtagcaatc
     5581 atgaacatag gatagatcga aagcatcgaa atatctattc tcatcaactt gccgatgatc
     5641 aaaaataccg caagggctcc cgtccattgt ccaaaggatg ccagtagcag accaagcaag
     5701 ccttttggat aaaactcgaa gaacaatgtc tttctcttta gataagcaaa aaagaaaatg
     5761 actggtgcta aaaaactacc gccgaaaagc cagatccaat agcttcgaaa aatactttct
     5821 gactgaataa aaaagacatc aataaatgta agaaacgacc atacagacaa accagtcagc
     5881 ataaatagtg cgacttttga tacagtggct aaaaccattt ttctatttgt ctgctttcca
     5941 taaaaactag cccgcagact tgctcctacc acaccaccaa aaccggcgag attgttgatc
     6001 gtgtttgtta cccacgcagc aagaagaaac tcttttcttt ccatttttgg ttttccttgt
     6061 ttttctaaga ctctgatcgt cacccaatca tacaacaaca tcggagtgac acccagcagt
     6121 ccagccagaa tcatgagcag gatcgtcatg cggctttgtt gtcccatcct ttgccaaata
     6181 tcttgccaac tcatgccatt agcaatattt gcgacttgat ttgccacaaa gactaacacg
     6241 gagccgaaaa aaatcagttt taaaatcaga ctatgttcct ttatccattg cagcatcaag
     6301 tatcgtttat ttttcacatt ttcccattcc taacttgtat acttttgtgt cataccccaa
     6361 tgttatctta tttattttaa tttttccact ttagacctgt tctttgcttg cgatgataga
     6421 aaaagaaaga tatcctaatg ctaaaggaga ggataacatg aaagtaggat ttatcggcac
     6481 agggaatatg gcacaagcta ttattgatgg tttattaaaa aaaggaacag tctcgaaaga
     6541 agaggtgttc gtttccagtg gacattttga aaatgcacaa aaatttgctg ctgaaactgg
     6601 cgtccatgca tgcaaaagta atcaggaagt tgcatcacaa tcagatatcg ttcttttagc
     6661 cgtgaaacca aaaatcattg aaaaagtatt ggaagaacta agagaatata gccagaaagt
     6721 cttattcatt tctattgctt caggaaaatc attaacagat ttagaaacag cgcttgaaga
     6781 tcctcaggcc tccatcgtac gtgtgatgcc gaatgtcaat gtcagcgtag gagcaggcgt
     6841 ttctgcgatt tgcaaaaatg aagctgccac ggaggaacag cttcagcaag ctacgatgat
     6901 ttttgaagca atcggcagcg tctatccttt agcagaatca gatttcagta cattcattgg
     6961 ccttgctggc agctcacccg cattcgttta catgctgatc gatgcgatgg gacggactgg
     7021 tgtactgaat ggtttgtcca aacagacagc cactgagatt gctgcaaaag caattatggg
     7081 ttcttgtcaa aaattccttt caagtgataa aaatccatgg gaacttgtag accaagtttc
     7141 ttctcctggc ggtacaactg ttgcaggtgt agttgcctta gaagaagcag gattcatccc
     7201 agcaatcatc aaaggaatca atacgacgat cgagaaagat cttgagatgt tgaataaaaa
     7261 ataatgatag caatgttttt tcgtgcctta caaaaaaaga agagctgtgg cttttgccgc
     7321 ggctcttctt ttgtattttt tattatttag cataatcagt tgcacgagtt tcccgaataa
     7381 cgattacttt gatgtgtcca ggataatcca gttcttcttc gattcgttta cgaatatcac
     7441 gtactaaacg aacagcgtct aaatccgaga tttcttctgg cttgaccatt acccgaactt
     7501 cgcgtccagc ttgaactgcg taacttgatt ctacaccggc gaagctgttc gaaatatttt
     7561 ccagacttct taaacgacgg atgtagtttt ctaatgattc acttcgcgca ccaggtcttg
     7621 ctgctgagag cgcatctgct gcagctacaa ggacagagat aacagaagta gcttccacat
     7681 ctccatgatg agaagcgatc gcgttgatta ctactgggtt ttctttgtac ttggcagcta
     7741 attccgcacc gatctcaacg tgagatcctt caatctcatg gtcaagtgct ttcccaatat
     7801 catggagcaa cccggcacgt tttgctaatt ggatatcttc gccaagctct gctgctaaga
     7861 taccagctaa ttttgcaacc tcgaccgaat gcttcaagac gttttgtcca taactagttc
     7921 tgaatcgcag acgtccgatg atcttgatta gatctggatg aagtgtatga gcaccgactt
     7981 caaacgctgc ctcttccccg tattcgcgaa tacgttcatc catttcttta cgagatttct
     8041 cgaccatttc ttcgatacgc gctggatgga ttcgtccatc ttgtatcagt ttttccaagg
     8101 tcatccgtgc gatttctctt cggattggat cgaatccgga aagaacaact gcttccggtg
     8161 tatcatcgat gattaaatcg ataccagtca gcgtctcaag cgttcgaata ttacgacctt
     8221 ctcgtccaat gatacgacct ttcatttcat cattcggaag ggtaacgact gaaacagtcg
     8281 tttctgaaac ttgatctgct gcgcagcgtt gaatcgctaa tgataataag tttttcgctt
     8341 tgcgatctgc ttcttctttg gcacgctgtt cagattcttt taccattaga gttaactcat
     8401 gattcagctc ttcttcagtc gatttcatga tgacatctcg tgcgtcctct tttgataaac
     8461 cagcaatttt ttctagttct ttttgttgat cttcaatcat tttttcaacg tctttttctc
     8521 gctcttcaat taattgctgt tttgaactaa gtgcttcttc tttactttca agtgagttct
     8581 cgcgtttttc taacgagtca tctttacgat tcagcgtttg ttcgcgttga atcaaacgat
     8641 tctcttgaga ttttaattct aacttacttt ctctcagctc actttcaact tcagaacggt
     8701 atttctgatt ttcttcctta gcttctaaca atgcttcttt ttttagagtt tctgcttctt
     8761 tttttgcact atcgatgatt ccagcagctg atgattgtgc accagctatt tctttctcgt
     8821 gacgtgactt tgcgaccata aaccctaaac caagaccgac aattaaaccg atgatagcga
     8881 ggagaatgtt gaataccata aaatccacct ccatactatc tttttcattt ttatcatcta
     8941 tacagcttct atgataaatt attctaaact cgtcaatatg catcttcgca tatatgtgta
     9001 caaaaaacat gcacaacttt attctaatgt ttgtaccccg tagtgtcaac tttaaataaa
     9061 caaagaaacg gctttctact tattcattaa acgatggttt ttaaaataaa tgacactagt
     9121 ttcatatagg taaaccttta aattgagaaa cttttctatt aaattattct ttgtagatga
     9181 acagatattt ttattttatg tacccactcg tataattaaa taactcacga tttctttatt
     9241 caataaaaaa acggcaggtc aaggagagta cctactcttc agaccagccg tttttgttta
     9301 tcataatttt tattcttcta ctaaaggcaa ttcttcttgt cctttttcat cttctgggac
     9361 agcgacttct tcaccaatac cataagctgc tctaactaga gcagaaactt ctgccatcat
     9421 ttcaggatga ttagccatat agattttagc gttttcccgt ccttgaccaa tccgatcttc
     9481 tttataagaa taccaagcac cgcttttatc aacaatgtct ttttctactg ccatgtctag
     9541 aagttctcct tcttgtgaga tccctaaacc atacatgaca tctacttcag cgattttgaa
     9601 aggaggtgct actttgtttt ttacgacttt gatctttgta cggttaccaa caatatctgt
     9661 accttgtttc aattgttctg cacgacgtac ttccaaacgg atcgttgcgt agaatttcaa
     9721 tgcgcgtcct ccaggagtga tttctggatt cccaaacatc acgcctactt tttcacggat
     9781 ttggttgatg aaaatagcga ttgtttttgt tttgttgatc gaaccagaga gtttacgcaa
     9841 tgcttgagac atcaaacgtg cttgtaaccc gacatgtgag tcacccattt cgccgtcgat
     9901 ttctgctcgt ggaactaaag cagcaactga atcgactact acgatatcta cagccccact
     9961 tgatactaaa gcatcagcga tctctagacc ttgttctcct gtgtcaggct gtgaaagaag
    10021 tagttcatcg atattcacac ctaatttttg tgcatattgc ggatctaacg catgctcagc
    10081 atcaatgaaa gcggccgttc cgccattttt ttgtacttct gcaatagcgt gtagtgcaac
    10141 tgttgtttta cctgaacttt caggtccata tacttcgatg atacgtccac gggggtaacc
    10201 gccgacacct aaagcgacat ctaacgcaag agagccactt ggaattgtag agatttgttg
    10261 gtcgattttt tcgcccaatt tcataataga gcctttacca tagctctttt caattttttt
    10321 cagtgcagca tctaaggctg ctttacgatc atctgccaat acgataatcc tccttatatt
    10381 tacaaatcag agcaacattt cattatcctt atcatatctt ttttagtgaa aaaaagcaag
    10441 aaaaaaccga acaaatattc gcatttatca ttttcttaaa agctgttttc tcaaaagatc
    10501 aaatccttgc ataaccgcat tatatctgat agcagcacga tctctggcaa agtgatattt
    10561 ttccgaaacc acgccgtctt tagatgaaat agcgatccat actgttcctg cagattgacc
    10621 ttctaacgtg tccggtccag ccactccggt gaaagctaca ctgtaatctg ttcttgctat
    10681 ctcacgtgct tgaatggcca tctgctccac acattctttg ctgactgttc catattctgc
    10741 taatagctcc ggatcaatct ccagaaaacc agcttttgtt tggagcgagt aggtaacgaa
    10801 tccgcctgga aatacttcag atacaccagc aatgtcgcct aatgcggctt ggaaggcgcc
    10861 ggctgtcaaa ctttccgctg ctgttacagt ctgcttgttt tctttcagta gttccactac
    10921 gacttttgct agcgaattat catcgccgta tccataaaaa tattccccta ctcgttcttg
    10981 gattttttct tctaatgcta aaagagcttg attccctgcc tgaacatcat ttgttttcac
    11041 tgtcagtcgt aatgtcactt catttggttt ggcatatggt gcgatcgttg gattgatctg
    11101 tgtctcaatc agatctttta gctcagtgac cagtcgcgat tcaccaatcc cataaaagcg
    11161 caaaacttta gaaattagtt tttcttcaga aggaaaacgt tcctctagta aaggacgtac
    11221 ttgttccaca aacattggtt tcaactcatt aggcggaccg ggaagaagga tatacgcatg
    11281 tgtatctgtt tgataaaaag tccctaaagc aagccctgta cgatttggta gaggaacccc
    11341 gccttcgata atctgggact gttgcagatt gtttttggtc atttttcgat gggttgtttc
    11401 aaaatatgcc aataattttt tgtatccttc cgtattttgt atgagagact taccgaggtg
    11461 agccgcagtt gcttcttttg tcaggtcatc ttctgtcggt cctaatccgc cacataagag
    11521 tatgagttca cttcttgttt cagctagagc aatcaactct tctaaacgct gctgattatc
    11581 tcctacaact gtttgataat aaacttcgat acctagatca gctagctgtt ctgacaagaa
    11641 agtggcattc gtattcacga cttgtcctag taaaagttct gttccgacag ctattatttc
    11701 tgctttcatt acttcccgct cccttatata gtatacgctg tatttttata tttttttatt
    11761 taaaaagagg gcaaggagct gtgacaaaaa tcttgtcaca gctccttgtt ccaaataaac
    11821 agtgttctcc tgccaaaacc tcggtcataa gtcacaatat acataaaaca tgaaaagctg
    11881 ttttttgaaa ttatcccttg tgactcagag ttagacggca ggctcacaac ctctccacta
    11941 aacgatactt acattgatcc tttgaacaca ccgctgtttt tgatgaagta atccacacct
    12001 gaatagatag taaagatcag acaagcataa agcatgattt gatctactgg aataccgatc
    12061 aggctgaatg ggatattatt gataaacagc aagataatag caaccatttg tgtagccgtc
    12121 ttgacttttc ccggccaagc tgcagccatg acttctccat gttcgacaag tagtaaacgc
    12181 aagcctgtga cggctaattc acgacagaca atgatcgcta ctacccaacc aggggccttt
    12241 ccttggtcaa ccaacatgat aaaagcagtc ataactagca ttttatctgc caacgggtcc
    12301 gcaaacttgc caaaattagt gacaaggcct cgcgcacggg caatttttcc atctaaccag
    12361 tctgtgatac tagctaaagc aaaaataact gcacctacta gctgtgtgac agccagtgtg
    12421 gtgtctccta cgttaaattg tccccaatct aaaggtaaac ctacaaccaa aatgaaaatc
    12481 gggatcatac aaattctaag tacagttaat ttatttggta aattcacact atcgactcct
    12541 ttcttcccta ttctacgtga ctatcagcga agtttccacc gatattattt acttttccaa
    12601 atgaatttat tcaaaggagt atttttattg aaattgatct ttttgaatta acgaagaagg
    12661 accgcgacac agatttgtca cgatccttct tttttatacc tacaagtaaa cagatgcgta
    12721 aactaattta tacgatcatt ctgcgtattg aagagtaagg ttcacatttt ttggactctg
    12781 attgttttcc cctggattaa ctggtacgga ttctccattc gctttgatcg ttgcattgct
    12841 tgctgcacca atcgtgatgg tcgcatttgt tgctgtttcc ggtaacacgg tactctgtgt
    12901 ttcatttgct gccagtgtcc cttgataaac ataggcattg tctactagca cacctaacca
    12961 tacacgattg acagcagtaa attcaagggt tacaggcttg gtagcgtctg tgatatttac
    13021 tgcgatggca ctgccagaat cttctcctgt agctattgcc attttctttt cttcacttga
    13081 ttttgttgtt gattctactg tagaagactc tttggtctgc gtgctttgct ctgaggaaga
    13141 ggactcctcg actgatcctt ctactttaag cgaagaagct gtgtctccga ttattggatc
    13201 agcttgacga tcttgccaag tcatatagcc cacgacaacg acaatcgcca aagcaaccaa
    13261 tcctaataaa atgacaggaa gacttgtcca aaagcgactt ggatgcgatt cttcctgatg
    13321 cattgctttt ctagaagtac ttactgtttc aggctgcgga cgttcaggga ccgatcctgt
    13381 caatttgctt ttcccatcaa aaacatcgac taaatggtct ccatcttctc ctacagcttc
    13441 agcgtattgc cgaataaatg cccgcacgta aaatttacca ggcaattggt caaaatcatc
    13501 attgtcgatt gcttccaaat aacgtttttg aatttttgta atctgctgta attcatctaa
    13561 agatattttt ttattcaggc gtgcttgccg taatttttct ccgatggttt caaaagccac
    13621 tcgttcatct tctccagtct gtttgattct ccgaagcatc tgctgttgca acgaaagacg
    13681 ttatgtttgg gtgctgtgac aaaagtcatg tcacagctcc ttttttccga ataaacattg
    13741 ttctcctgcc aactcctcgg tcacaagtca caatatccaa taaatataag aagctgtttt
    13801 ttgaacttgt tccttgtgac tcggagttaa acggcaggct cacgacctct ttcttaaacg
    13861 gtgttcaatc agctgttcct cggtattagc tcaaaaaccg gcaaaatata aggagatatt
    13921 tttccgtatg tttctctaat actcaggtca aaacgctgat ttcacaacct cttccttaaa
    13981 cggtgttcaa aagcttgcat ctgcggtaat aagtccaacc aagttaaaaa cattaagaag
    14041 ctgtttttac ttggctgttc ttattacttg atgcagggca gctttttcac aacctcttgg
    14101 tacacggtgt tccagaagta gcttcttcgg caataagccg aaatatcaca aaaatttgag
    14161 aagcaatttt cgtaaattcc ttcttattt
//
LOCUS       DAKBVG010000059        14116 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000059 DAKBVG010000000
VERSION     DAKBVG010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14116
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.059"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            57..716
                     /locus_tag="OUD09_001224"
     CDS             57..716
                     /locus_tag="OUD09_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HCT9692503.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     CVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVS
                     I"
     gene            801..1706
                     /locus_tag="OUD09_001225"
     CDS             801..1706
                     /locus_tag="OUD09_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HCT9692504.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWEIALIISLVLSIWMLIALWRRIR"
     gene            complement(1708..2340)
                     /locus_tag="OUD09_001226"
     CDS             complement(1708..2340)
                     /locus_tag="OUD09_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCT9692505.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGNQSYLDAKN
                     MVLRESHIQKLTMQKTGLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTTIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     misc_feature    2491..2616
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            2660..3184
                     /gene="infC"
                     /locus_tag="OUD09_001227"
     CDS             2660..3184
                     /gene="infC"
                     /locus_tag="OUD09_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCT9692506.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            3256..3456
                     /gene="rpmI"
                     /locus_tag="OUD09_001228"
     CDS             3256..3456
                     /gene="rpmI"
                     /locus_tag="OUD09_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCT9692507.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            3509..3868
                     /gene="rplT"
                     /locus_tag="OUD09_001229"
     CDS             3509..3868
                     /gene="rplT"
                     /locus_tag="OUD09_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCT9692508.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            4304..4645
                     /locus_tag="OUD09_001230"
     CDS             4304..4645
                     /locus_tag="OUD09_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9692509.1"
                     /translation="MLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLIRKV
                     VELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVKSTILCKS
                     STSSKAQCFAL"
     gene            complement(4752..5939)
                     /locus_tag="OUD09_001231"
     CDS             complement(4752..5939)
                     /locus_tag="OUD09_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HCT9692510.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
     gene            6036..6932
                     /locus_tag="OUD09_001232"
     CDS             6036..6932
                     /locus_tag="OUD09_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HCT9692511.1"
                     /translation="MDVIGISVPVALFSFMGSIMAGIFANIVNFKQYQTIYAGFNPAA
                     ESYTYNDYFQIGMIGMIVSLVVVLTVANISMNKKKRYAMAANVPAEGGDAPMISWLAV
                     LLPVLGVVLLDLQIILGFILAGIWALLFTGKLRGGYKEICRQFAKLFTDGAVDVAPMV
                     GFLMTLAMFNNSAAYASPYFSAIFGDWIPQSPLVLAIVFAILTPLGFFRGPMNLVGSG
                     SAILAVVLAVNPTMSPAFLFPLFAITTIAPQHLDITQSWVAWGLGYTKVTSREYMKKS
                     IPTGWIIGAILCLITFLLYGNA"
     gene            6980..9109
                     /locus_tag="OUD09_001233"
     CDS             6980..9109
                     /locus_tag="OUD09_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HCT9692512.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKNLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKAIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPHTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            9084..9773
                     /locus_tag="OUD09_001234"
     CDS             9084..9773
                     /locus_tag="OUD09_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9692513.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKEIE
                     KRCTEYDLDYK"
     gene            complement(10181..10867)
                     /locus_tag="OUD09_001235"
     CDS             complement(10181..10867)
                     /locus_tag="OUD09_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCT9692514.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(11003..11170)
                     /locus_tag="OUD09_001236"
     CDS             complement(11003..11170)
                     /locus_tag="OUD09_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9692515.1"
                     /translation="MYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKESEAFAKRIGG
                     RVKPIQEVRPD"
     gene            complement(11247..11687)
                     /locus_tag="OUD09_001237"
     CDS             complement(11247..11687)
                     /locus_tag="OUD09_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HCT9692516.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELEQLFQAYASLDDVNS"
     gene            complement(11691..12536)
                     /locus_tag="OUD09_001238"
     CDS             complement(11691..12536)
                     /locus_tag="OUD09_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HCT9692517.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVDWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLVANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAVYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            12685..14103
                     /gene="pepV"
                     /locus_tag="OUD09_001239"
     CDS             12685..14103
                     /gene="pepV"
                     /locus_tag="OUD09_001239"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HCT9692518.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
BASE COUNT         4416 a         2626 c         2936 g         4138 t
ORIGIN      
        1 tttaaaaaag gttggagtta aacacaatat ttagtgatga agtgagctgg aggtaaatga
       61 gcgtgatcgt tttagcaggt acaattggtg ccggaaaatc aagtttaaca gaaatgatgg
      121 ctgaacactt tgatagtcag gcattttatg aatcgattga tgataatgaa gtcttgccct
      181 tattttatgc aaaccctgaa cagtatgctt ttttgctgca aatttatttt ttgaataaac
      241 gttttgcaag catcaaacaa gcaatgaaag atgacaataa tgtattggac cgttcgattt
      301 atgaagattc actgcttttc catctaaatg cagatctagg aagagcaaca gaaacagaag
      361 tttgtgtgta tgatgaactg ctggaaaaca tgatggaaga acttccctat gctgcccata
      421 aaaaacatcc ggatttatta gttcatattc gtgtatcttt tgatacgatg ttagagcgta
      481 tcgaaaaacg tggtcgttcg tatgaacaat tgtcctttga cccaagtcta tacgattatt
      541 acaaagaatt gaacagacgt tatgatcaat ggtatgaaga atacaaagaa agtccaaaaa
      601 tccaaatcga tggagaccgc tataattttg tggaagatcc tcaagcaaaa gaagaagtct
      661 tgaaaatgat cgaagaaaaa ttagcagaga ttcgccaaac caaagtatcc atttgattta
      721 cagttgctca aataaagggt aaacctttat ttgagcgact tttttcttta tactggaaac
      781 aaatgtcagg aggatagagt atgcgggaaa aacactattt taatcaagac cgtaattttt
      841 ggatcaatgt acagcaaaac gatgtgctag ctgttaaaaa cttaaaagaa gaatatggca
      901 ttagtgacga gatgctgact tattctctag ataaaaatga acgggcgcgc gtagagtacg
      961 attcttttga tgaagcgtta ctgcttgtat caaatgttcc gcaccagcaa aaagtcgata
     1021 atcattatga aactagtcct atcgctttta ttttaaaaga agacggtttg tttacattta
     1081 ccacgccgaa taccgagtac gtgattcgac taattcgatc gcttttggaa cggatgccag
     1141 acatgagtgt atacagttta ttgtttcgta cattgttttt gatttctgat tccttttttc
     1201 ctttaatcga ggaagtgaat agtgaacgtc agcggcttaa tctgaaatta agagaaaaaa
     1261 caacgaacaa gaatctgctg caactttcag atttagagat cggtttggtt tacttagtta
     1321 caggaacaaa acaaaatgta gtacttttag aacaaatcaa ggcactggct atttatcgca
     1381 agttgtcaga aaaggaaaaa gaagagttgg acgatgcact gatcgaagca cgacaagcag
     1441 ttgaaatgac gaacttagct tcgcagattt tagaccagct ctctggcact tataataatt
     1501 tgctgaataa caacttgaat gacacgatga aatttttgac ggtctggtcg ctgatcttga
     1561 ccgttccaac tattgtgaca ggattcttcg gaatgaatct ccagttaccc tttacccatt
     1621 ccgttttcgg atgggaaatc gccttgatca tcagtttggt cttatctatt tggatgctta
     1681 ttgcactgtg gcggcggatt cgttgatcta gtctttgatc tgcaaaccaa gggcttgggc
     1741 gatcacaagt gcttgctctt gattgacgat tagtcccgcc aaagaatctt gagataaagc
     1801 gattgttgtg aagaaattag tcgtaaagtc taatcccttc agagaagttc gaaaccagtt
     1861 cgaaccggtt aactgattgt cagacaaaaa aaggtttttc caagtgacct cataaaattc
     1921 gctgtcttct aattgacaat gatcgaagga gacagctttc aagtttgtac tactaaagga
     1981 agccatcgaa gcaatacaat cagtgaaagt acagtctcga agataacttt cggcaaaatt
     2041 cgtacctgtt aatttacatt gatgaaacac gacttggtgg aaactgccac caatccactc
     2101 cagattggaa aaatcacatt tttcaaaaat gacattactg cattcaaaat gacgtaaacc
     2161 tgttttttgc atcgtaagtt tttggatatg actctctctt aataccatat ttttggcatc
     2221 gagatagctt tggttacctc cgttgatcag aagaccagag aagatctcct catcttctaa
     2281 ataaaactct ccctttgtaa gaagaggaag agaaggtgga ataggttcga tttttttcat
     2341 gtgggttcac ctcgtttttc tttagtatag caaagaagcg ggaatcactg ctagaaagcc
     2401 tgctaatcta atcattttca gaaaaggaag acaaactgta tgaaaaacat tgacaaaaag
     2461 attagttttt gctatactat aatggttgag aaataaaagc agaagcaccc gcttctcgcc
     2521 ttagttgata atgtcactgg gctagatagt tttcactggt tctattaggt gaagtacgcg
     2581 cgggttctgt aagagaactc gtttttttat tgccgtttat tcggtaagct tttctttctc
     2641 tcataatatt tggaggtgaa tgaccatagc aaaggatatg atggttaacg acggcattcg
     2701 tgcacgagag ttacgattga tcggttcaga tggtgagcaa ttaggagtta agacaaaagc
     2761 agaagcattg aatattgcag aacaagcaaa cttggatctt gtgttagttg cccctggtgc
     2821 gaaaccacca gttgcgcgaa tcatggacta cggtaaatac cgtttcgaac aacaaaagaa
     2881 agaccgcgaa gcgcgtaaaa aacaaaaagt gatcaatgta aaagaagttc gcttaagtcc
     2941 tacgatcgac ttgaatgact tcaatacaaa acttcgtaat gcacgtaagt tcttggaaaa
     3001 gggcgataaa gtgaaagctt ctatccgttt caaaggccgt gccattaccc ataaagaaat
     3061 tggtcaaaaa gtcttgaatc gcttagctga agaaactgcg gatattgcaa cagtggaaca
     3121 aaaagcgaaa atggacggac gcagcatgtt cttgacgctg gcaccgaaaa acgacagtaa
     3181 gtaatttgaa tgactaactg gctgttggac agccgatagt cggataacga aagattttga
     3241 ggaggaaata tagtcatgcc aaaacaaaaa acacaccgcg gatcagcaaa acgtttcaaa
     3301 cgtactggta aaggcggatt gaaacgcttc cgcgcgttta caagtcaccg tttccacggc
     3361 aaaacaaaaa aacaacgccg tcaattgcgt aaagcaagca tggtttctag tggcgacttc
     3421 aaacgtattc gccaacaact agcaaaaatg aaataagaag tacaaataac agaatctgaa
     3481 gatttatttt tattaggagg aattatacat ggcacgtgtt aaaggtggaa cagtaactcg
     3541 taaacgtcgt aaaagagtgc taaaattagc aaaaggttac tacggttcaa aacacacttt
     3601 atttaaaaca gcaaaagaac aagtaatgaa ttcatacaac tacgcatatc gcgatcgtcg
     3661 tcaaaagaaa cgcgacttcc gtaaattgtg gattgctcgt atcaacgcag cagctcgtat
     3721 gaatggcttg agctactcaa aattaatgca cggtttgaaa gttgctgaaa tcgacatcaa
     3781 ccgtaaaatg ttagctgact tggctgttaa cgatgcagca gcatttactg cattggctga
     3841 ccaagcaaaa gaagctttgg ctaaataagc taaacaatta taaagaggaa agaagataac
     3901 gaaaagttat gttctttcct ctttttttat ttacaatcct ccatcaaatg aaacaggaca
     3961 ataacgtata agtctaaaat aacataatta aggtttttta attgaaaact gaacatttat
     4021 ggtcatcctt taatgaaagc gctaactaca taagttagaa tgagtttgac tgatatatta
     4081 gtttgcttat cagaaaaaca aaatggacgg aggaataaca tgagtgatat tattattggt
     4141 attttgttag ttatctcatt ctttttcatg gtctggtact gtgtaaaagg ctacaacttg
     4201 atggtcggct tttgtcatca tggctactgt atggatggga ttagcgctag tcggaaacac
     4261 gttttcacca aactcggcga tggaagggca aggagtaatt gatgtgttga cgcatatcta
     4321 tacgacgggg ccagctgagt acgccaaatc gatcttggta aatgtcttct ttggtgcctt
     4381 tttcgggcgt gttctagtag atagcggaat cgcagctacc ctgattcgaa aggtagttga
     4441 acttggaggg gacaagccaa gaatcacgat gagcttacta tgtattgtaa ccgctgttat
     4501 attcatgtcg atgacaggaa tcggaccagt gatttctatt gcagttattg tgttgccgat
     4561 cttgatgtca ttaggtattt cagtcaagtc cacaatcttg tgtaaaagtt caacctcatc
     4621 aaaagcacaa tgttttgcgt tgtgaaccta actgccatgc ctttctttct gaggagagag
     4681 gtcaggagca gtagcgcagc gactcttgat gtctctcctc agaaagaaga gactcgcaca
     4741 ggttcatggt gctaaatgtt attcgaatta aattctaaat attttcgtgg agaagccagc
     4801 cactcagtgt ctttttccat caaaagtgtt ccaattatcc gagtgatgga attgatattt
     4861 ggaaaaatcc gaatcactcg ttctctccgg cgaatctctt cgttcaaccg ttccaacaca
     4921 ttggttgtac gaatccttcg gcggatattt tcaggaaaag acaaaatttg aatagcatct
     4981 tcaaagccgt tttctaaaat gtcgcaagca gcacttaatt ttgaatcaca accatatttt
     5041 tccaagaagt gctcttttct ttcacgtgtc aattcaagtt cagaggcttt aaagatactc
     5101 ttcaactcgt cttttacatc agaagaaact ttctttggca gcttatcaaa aatatttctc
     5161 aaaaagtggg cttggcatct ttgccagctc gcacctaaaa agttcttgcg aacagcctta
     5221 actaaccctt tgtgtgcatc agaaatgacc agttttacac cgaacaaacc tcgttgaacg
     5281 aacgattgaa aaacggtatc ccaattatct tccgattcgc cgtcttgaac atcaaaacca
     5341 agaatcgtac gtcgtccatc agaatcaatg ccgataccaa tatatacacc ttttgagaca
     5401 atacgatgat tttcccttac tttcatatac agggcatcac acatgaaaaa tggatattcc
     5461 gagccttcta ggctccggtt tctccactca aaaacaaatg gatccaactg tttcatcaga
     5521 tttgaaacga atgatttaga aacagtcgca ccatccgtta gcagttcaac tgttttagtt
     5581 actttgcgag tggagacacc agaaatgacc atttcaatca tggcagcgat caaagctttt
     5641 tcgcttcgct gatatctttc aaagatagta ggactaaact taccgtctct ggttcgtgga
     5701 acatgaagtt ctaacgtacc aattcgagtt gtaaacgaac gatcgtagta accatttctt
     5761 tgtgattgcc gttcttctga acgttcatag gcatttgcct gaatatattg atttcgttgt
     5821 tcttccatca gctgattaaa catgatagtc ataagtgttt ttgcggtatc attcgcaccg
     5881 ctttcgttta taattgcttg aatttcttcg ctttcaaaag taaaatgtac ttgagtcatt
     5941 gatatcagtc ctattcgagt atgttttctg caaaaaacat tgtactccta actgagatca
     6001 atggcttttt tgatgcttca cttttacaca attatatgga cgtaatcggt atttcagtac
     6061 cggttgcact gttctcattt atgggctcga ttatggctgg aatctttgct aatatcgtca
     6121 atttcaaaca atatcaaacg atttatgctg gtttcaatcc agcggctgaa agctatactt
     6181 ataatgacta tttccaaatt ggtatgattg ggatgattgt aagcttagtt gtggtactaa
     6241 cggtcgctaa tatatcaatg aacaaaaaga aacgctatgc aatggcagca aacgtaccag
     6301 ctgaaggtgg cgacgcacca atgatctcat ggttagccgt attattacca gttttagggg
     6361 tcgttctttt agatcttcaa atcatcttag gttttattct agcaggtatc tgggcattgc
     6421 tgtttactgg aaaattgcgc ggaggttata aagaaatctg tcgtcagttt gcgaaattat
     6481 ttacagatgg cgcagtcgat gttgcaccaa tggttggttt cttgatgacc ttagcgatgt
     6541 tcaataattc agctgcgtat gcttctcctt atttttcagc tatttttgga gattggatac
     6601 cgcaatcacc attagtcttg gcaatcgtat ttgctatatt gacaccacta ggatttttcc
     6661 gcggtccaat gaatctagtc ggttcaggct cagctatttt agctgtcgtg ttagctgtta
     6721 atccaacgat gtcgccagcc tttttgttcc cgttatttgc aattacaacg attgctcctc
     6781 agcatttaga tattacacaa tcttgggttg cttggggatt aggttataca aaagtaactt
     6841 ctcgagaata tatgaagaaa tccatcccaa ctggttggat catcggtgcg atcttatgcc
     6901 tgatcacgtt cttattgtac gggaacgcct agtaaacaaa acgtcgataa aaacgattag
     6961 ataaatggag gaaaagaaaa tgaaacgttt tgtgcgaatg ggaattgatg ttggcggtac
     7021 acatacgaaa gcagtcgcaa tcgataatgc gacgcacgaa atcatcggaa aatcttcagt
     7081 gaaaacaacc cacgatgatg tccgtggagt agctgcaggt gtcgttcagt cttttcaaaa
     7141 ttgtttgcgg gaaaataata tcagtccaga agatgttgtg tttgttgcac attctaccac
     7201 acaagcgact aatgcactga ttgaaggaga cgtagcaaaa gtcggcgtaa tcgggatggc
     7261 aaaaggtgga ttagaaggtt ttttagcaaa acgacaaact agattgaacg atatcgattt
     7321 agggaataag aaaaaaatag agattgtcaa tgcattctta cctgtcaaaa acttaaatgt
     7381 agaccgtgtt tcagaaacga tttcttcttt agaaagagaa agagcggagg tcttggtttc
     7441 ttctatggca tttggagtag ataatgggga accagaacga gttgtttatg aagcagcatc
     7501 tgtgaaagcg atccctacaa cgatggcttc tgacatcaca aaattatatg gattgacaag
     7561 aagaactcgg acagcagcaa tcaacgcatc tattttacca aaaatgctag atacggcaac
     7621 ctcgacagaa gactccgtac gagaagcagg ggtcaatgtt tctttgatga ttatgcgcgg
     7681 agacggcgga gtaatggaaa tcaatgaaat gaaaaaacga cctgttttaa ccatgctttc
     7741 cggtcctgct gcatcagtca tgggctcttt gatgtacttg agagcgtcta atggtgttta
     7801 ttttgaagta ggcggaacaa caaccaatat cggggtcatt aaaaatggac gtccagcaat
     7861 cgattattca attgtaggtg gccacccaac atatatctct tcattagatg ttcgggttct
     7921 aggtgtggca ggcggttcga tggtacgggc taatcagtca ggaatcatcg atgtcggtcc
     7981 acgttctgca catattgccg gtctagatta tgcagtattt acggagacag aaaaaatcaa
     8041 aggtcctaag gttgaatttt tctctcctaa agaaggagac cctgctgatt acgtcaaggt
     8101 agtgatggaa gacggagaag aagtgactat tacaaataca tgtgcagcca atgttcttgg
     8161 attagtgcaa gaagaacatt tttcttatgg aaatgtccca tctgcacgaa aagccataca
     8221 ggcattagca gactattgtc atacgacggt tgaagacatc gcagagcaga tcatggaaaa
     8281 atcctatgct aagattgaac cggtgatttt agaattggca gacaagtatc acttggagaa
     8341 agatcagatt tctcttgtag gtgtaggggg cggcgctgcc tcattgatta cttactttag
     8401 taataaaatg ggtgtgaaat attcgattcc tgaaaacgcc gaagtgattt cttctattgg
     8461 tgtagcactt gcaatggtcc gagatgttgt agaacgtatc atcccttcgc caagtaaaga
     8521 agatatacga tcgttgaaaa atgaagcgat gaataaagcg attgaatcag gcgctacacc
     8581 agaatcgatc gaagtccacg tagaaatcga tccacatact tcaaaagtaa cggctatcgc
     8641 tactggttct acggaagtga aagcaaccga tctaaccaag gagatcacaa cagaagaagc
     8701 actagagctt gctgcagagg atatgcgatt aaataaaaat gaagtttgct tattggaaaa
     8761 tacacctttc ttttatgtat gcggcgaaca gaatcgttca aaaaatgctg gaagtcttcg
     8821 gatcatcgat caaaaaggat ttatcaaggt ccaacgtgga catgcttcat gtatgaaaac
     8881 aactgcagcc aattatatga cagctgtcga acaactttgg gaagatatgg cagtttatca
     8941 aactgaattg attgcccgac cagaatttta tctctgctta ggagcaagag tctctgattt
     9001 tacagcgacg gatctggaac aactacagct tttgatggat ctggaagtat ctactatgga
     9061 accggaggaa gaagtgatcg tcgttgccgg aaatatcaaa caaacataag ttagaagagt
     9121 tactaaacga attacaacag atccccgaag aaatttgggg attctaccag tttcggcgag
     9181 atttattttg gaaaaagatt cctttgtcca agcagaaaat tcttatacaa caatcaatcg
     9241 attgtggtat cgaaacggca tgtagcatca aaaaaaagta cccttttgcc gatgtaggag
     9301 aaatctgtga acaaatggct gttccaatcg tttcctgtga atcagagcaa ataaacgaac
     9361 gaatcacttt tgcaacttat gcagaagatg aagggatccg attgatgacg gagccgttag
     9421 aaaaattaaa atgttcagga ctagcttcga tctctaaaga aacagcgcaa gctttgatta
     9481 ttggacatga actatttcac cacatcgaag cttctgttaa gggaatttat acgcaaagcg
     9541 aaaaaattgt gctgtggcgg ttgccttttt atacacatca atcaaacatt cgtgcattga
     9601 gcgagatagc agcgatgagt ttttcaaaag agatgaatca aagcgatttt tcgccttatg
     9661 tattagaggc agtcttactt tggccatata atgaaattcg tagccaaggg atcttagaag
     9721 aaataaaaga aatagaaaaa agatgtacgg agtatgattt agattacaaa taaagagact
     9781 gtgacaaaag tcgtgtcaca gtcctttttt tccgaataaa cggtgttctc ctgtcaactc
     9841 ctcggtcaca agtcaaaata ttcaacaaac atgagaagct gttttttgaa attgttcctt
     9901 gtgactcgga gatgaacggc aggctcacga cctcttggaa aaaggtgttc aaaagcttgc
     9961 atctgcggta ataagaacag ccaagttaaa aacataagaa gctattttta cttggctgtt
    10021 cttattactt gatgcagggc agctttttca caacctctca ataaacggtg tgcaatcagc
    10081 tgaccctcgg tattagctca aaaaaaacgg caaaatatga ggaactattt tgccgtttat
    10141 ttctctaata ctcaggtcaa aacgctgatt ccaaaacctc ttatttgatg ggtctgtcct
    10201 gaatatcttc aaaataatcg gggtaattat gaatcacgta ttcttgctcc tcgataatca
    10261 tatgtaaatg catcgtgcaa agaaatccga cttcatcaaa gttatgatca atcattgatt
    10321 gaagcattgt ctgatgttca tctaatactc gaccccagtc taattctgaa atcgtcaaac
    10381 gtaaccatct gaaacggttc aagtgcgtac tataactttc tagccaatcc caaatttctc
    10441 gttttccagc aatttcataa caagttcggt gaaaatcagt atccaaatca aaaaaagctt
    10501 ttttattttg agcgatcact gcctgatcgg tttgttctag tattttttct agtttagctt
    10561 gatcttgctt ggtcattttc gcactgcatt cttgcaggat cggtttttcc aacttttccc
    10621 gcgtataacg ggctaatgaa gcagatcgga tatcgatttt tgaaacataa gtgccggact
    10681 gtggaatggt ttttacaagc tcttgattgc gcagttggat cagtgcttct cgaatcggtg
    10741 tacgcccgat agctagttgt tcttctaaca ctttatcgga gattttactt ccgggtgcta
    10801 actcggaata aatgatttgt ttacgtatag attcataagc ttgctgttgc aaattattga
    10861 taaccattta accttccttt ctatgcagat ataatctatt ataacataaa tcgcttacaa
    10921 atcgtgtggg cataaaaaca cagtctgcaa aaaaagccct gataggcatg cctattagag
    10981 cctcgaaaag aaaaatattg aatcaatctg gtcgtacctc ttggatcggt ttgacacgac
    11041 cgccgattct tttagcaaaa gcttcggatt cttttttgtc tttgaaaatg agcagattgt
    11101 ctaagttttt ttcccgagtg cgacagccag ttttatcgat gtaaccgtgc attactttaa
    11161 cgacatacat ggggatcacc tgcttttttc ttttcattat acgaactact cgacacaata
    11221 caagaaaaat gcttagacgg tctttattaa gaatttacat catccaaaga agcgtacgct
    11281 tggaataact gttctaactc cggattgact ttgctcagct tcactaatcg ttgcgaatca
    11341 gaaagtaaaa aacagtgttt actactgcca gtagttttta attttttgtc ttcagttccc
    11401 acaatttcgt agctaaaatc caaccgtgtt cccgtatact tttggatagt tgggtggatg
    11461 ctcacttttt ctccgtaatg gatcatttct ttatagctgc aatttacttc cagaacgggg
    11521 atgatgatcc cagattcttc gattttttta tagggaaacc ctagatgtac caataaggct
    11581 acccgagcct cttcaaacca gcggatataa ttgctgtgat gaataatccc catttgatct
    11641 gtttcataat aaaaaggttc tcgcacataa cctgtaaatt tctccatgac tcatgcctcg
    11701 ttttccattg attttttaca aaaataattc atccacttcg gaagtgcttc gctgatttgt
    11761 gtcggcaaaa caatatattg ttcttttgcc agctggtctc caatgaaact atgaagataa
    11821 acagctgctg caatcgtttg gtcattttga ggaaactggg ctaaaaatcc tgtgatcatc
    11881 ccagctaaag tgtcacccat gccgcctgtt gccatagcag cagaacctaa aggattggtg
    11941 tagaagtgct ggtgaccata aatttttgtc tcatgacttt tcaaaacgag cacagcaccg
    12001 atttcctgtt gcttcttttt gttttcttta tcccgttgtt cttgtatcgg tagagaactc
    12061 aaccgttgcc actccatttg atgaggtgta agtacagttt gttcgggata gggtagcaaa
    12121 agatgattag caacaagtaa acttaaagca gagccatcaa tcaccagcca ttgttctttt
    12181 gattggtttt ctagtacttg tttgaaacgt tccaaactct tagaagaagt acccaatccg
    12241 ggaccaatca agagtacatc tgcttgcttt attacggaaa gaaactggtc agtatccgtc
    12301 caatctacaa tcattgcttc tggcagatga ctgtgaagag cactccgatt tttttcatca
    12361 gttactacag tcgtgagacc cgctccactg tgtacggcag ctaaagcact cataatgatt
    12421 gctccgccat attgttcatt gccgccaatc aaaacgatac gtccaaatgt tcctttatgt
    12481 gaaatctttg gacgaggtgt gatgactctt ttcactagtt gttcgtttat cagcatcgaa
    12541 aaacctcctt ttactcatta tagcgtatat tttttttaga gaaaaacaga taagaaaagg
    12601 aattgtcaga ttttttgaac tatttttttc tgtaatttgt ggtatcatga acacgaagac
    12661 ttaaataagt ggaggattaa ctttatgaca attgattggc aaaaagaagt agaagcacgt
    12721 aaagaagact tgcttgaaga cttgaaaaat cttttacgtg tcaacagcga gcgcgatgat
    12781 tcaaaagtaa caccagatgc accatttgga cctggacctc gcgacgcatt gaaacatatg
    12841 ttagcttatg gcgaacgtga tggatttaaa gtaaaaaatg tcgataacta tgcagggcat
    12901 attgatcttg gcgaaggaga cgaaacgcta gggatatttg gacacatgga tgtcgttcct
    12961 gcaggagatg gctgggacac agatccttat gaaccagtaa tcaaggatgg aaagatcttt
    13021 gcacgcggat caagcgatga caaaggtcca agtatggcag cttactatgc gatgaagatc
    13081 attaaagaac ttgacttgaa actatcaaaa aaagttcgct tcgtagtcgg cagcgatgaa
    13141 gaaagcggct ggggcgatat ggcttattat ttcgaacatg aagaagaacc agattttggt
    13201 ttctcaccag atgctgaatt cccaatcatc aatggtgaaa aaggaaatgt ttctttagct
    13261 ttacgcttca aaggcgacaa tgcaggagat tatgtcttga aatcttttgt ttccggactt
    13321 cgcgaaaata tggtgccagg aacagcaact gcagcacttc aagtaccaag tgcagatgca
    13381 gctatcgcga tggaagaagc attttaccaa ttcatcgaag caaatccagt aagcggaacg
    13441 attgaagcgg acaatacgta tgtaaaaatc gaattagttg gtaaaggcgc acatggtgca
    13501 agcccacaat ctggtatcaa tgcaggttct ttcttggcat tgttcttaga taactatgaa
    13561 ttcctaggat ctgctaaaca atttatccat gtagcagctg cttatgtcca tgaagatttt
    13621 tatggagaaa aactaggagt cgcttatgaa gatgaaaaaa tgggcaaatt gacaatgaat
    13681 gctggactat ttgcttttga agaaaatggt acagaggaag cgaacttcat caatatgaat
    13741 ttccgttttc caaaaggtgt aacagtagac ggattgcaaa gtgacatcga acaaacagtg
    13801 gggcaagaag gcgcaacagt tactcgaggt gctcgcgtaa tggaaccaca ttacgtacca
    13861 atggatgatc cattggtggc tacattattg caagtctatg aagaccacac tggagaaaaa
    13921 ggctacgaac aaatcatcgg tggcggaact tacggacgtt tgttgaaacg tggcgttgct
    13981 tatggagcga tgttcccagg atatactgat acaatgcatc aagcaaacga atttatgagt
    14041 cttgatgact tgttcagagc aacagcaatc tatgcagatg ccatctatcg tttggcgaaa
    14101 taaaaatact aaaaaa
//
LOCUS       DAKBVG010000060        13989 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000060 DAKBVG010000000
VERSION     DAKBVG010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13989
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.060"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(240..1967)
                     /locus_tag="OUD09_001240"
     CDS             complement(240..1967)
                     /locus_tag="OUD09_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HCT9692519.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(1960..3153)
                     /locus_tag="OUD09_001241"
     CDS             complement(1960..3153)
                     /locus_tag="OUD09_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HCT9692520.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3340..3984)
                     /locus_tag="OUD09_001242"
     CDS             complement(3340..3984)
                     /locus_tag="OUD09_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCT9692521.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4078..4212)
                     /locus_tag="OUD09_001243"
     CDS             complement(4078..4212)
                     /locus_tag="OUD09_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HCT9692522.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4214..6346)
                     /gene="feoB"
                     /locus_tag="OUD09_001244"
     CDS             complement(4214..6346)
                     /gene="feoB"
                     /locus_tag="OUD09_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HCT9692523.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6343..6816)
                     /locus_tag="OUD09_001245"
     CDS             complement(6343..6816)
                     /locus_tag="OUD09_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HCT9692524.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7217..7441
                     /gene="nrdH"
                     /locus_tag="OUD09_001246"
     CDS             7217..7441
                     /gene="nrdH"
                     /locus_tag="OUD09_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCT9692525.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            7600..9759
                     /gene="nrdE"
                     /locus_tag="OUD09_001247"
     CDS             7600..9759
                     /gene="nrdE"
                     /locus_tag="OUD09_001247"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCT9692526.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            9809..10774
                     /gene="nrdF"
                     /locus_tag="OUD09_001248"
     CDS             9809..10774
                     /gene="nrdF"
                     /locus_tag="OUD09_001248"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCT9692527.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(10863..12002)
                     /locus_tag="OUD09_001249"
     CDS             complement(10863..12002)
                     /locus_tag="OUD09_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCT9692528.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12311..13024)
                     /locus_tag="OUD09_001250"
     CDS             complement(12311..13024)
                     /locus_tag="OUD09_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HCT9692529.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13278..13979)
                     /gene="nagB"
                     /locus_tag="OUD09_001251"
     CDS             complement(13278..13979)
                     /gene="nagB"
                     /locus_tag="OUD09_001251"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCT9692530.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
BASE COUNT         4247 a         2916 c         2444 g         4382 t
ORIGIN      
        1 tttgagaatg cacttcgtac ataataaact ttaatcacat tagtctttca tacctacgat
       61 cgatctcttt gactcgcacc cataaattca taatagtttg tcggcgtctt tgaacgtttc
      121 gaagatgagc tacgcattct ctttttttat agacaaaaaa gatagagacg aacatggagc
      181 ttccaaattt ggatatttga tccaattttg gaagctccat ttatttcgtg tctgtctttt
      241 tattttttcc agctttcgac taattcttgg ttctctttga tccagtcttt tgctgcttgc
      301 tgtggatctt ttccattgtt gatttccaac ataacttttt ccatatcttt ttctgaccaa
      361 tggaaattgt ctaatacttt gtaagcttct ggctggtctt ctttcaatcc ttttcgaacc
      421 atcgtatgga tctgctcttc gcttcccatt ccgttttcag gatcttctaa gtatttcaga
      481 tcatatttgg caaacatcca atgaggtgtc catcctgtaa ctacaattgg ttcgtgtttt
      541 ttgatcgctt gacctagtgc aacggtcatt gctcctgaag aagatgtctc tactttccag
      601 tctttcaagt tgtcatattt ttggatcgtg ttttcagcag ccgtaactac accagctcct
      661 ggctcgattc ctatgatttt cttacctgcc tgatcggtta actcatcgat cgaatttaca
      721 tccatgtagg ctggcactgc taaaccaact tttgctcctg tcaaattggc acctaggtct
      781 tcgacttgat ctttatattg tgcatattgt gaaccatgag ttttaggtag ccaagctgaa
      841 accatggcat ctgactcgcc atttgaaaca gatttccaca tgattgaatt atctaatggc
      901 gtcatcgtta catcatagcc catttgtttc aatacttcac cgactacatt tgttgaagca
      961 acttctgtat cccattcaac ataagataag ttgatacgtt ttgtttcttt ggcagatgaa
     1021 aaactcccta taccaatcgc acctaagatc actactacta cagcaccaat gatcagtcct
     1081 tgacgttttt tacttttctg tgttgttgct cctgcagctt ttttattgtt tggctggttc
     1141 aaatgctgtg taaaacggtc aatgatgatt gctaagataa ctaatgcaac cccgttgaca
     1201 aatccattcc cgacttgtgc tctttgcaat gctgataata cgccgcgtcc taagccgggt
     1261 gcaccgatca tagaggcaat aacgaccatt gaaagtgaca gcattgttgt ttgattgatc
     1321 cctgccatga tcgtactttt agctaaaggc aattctagtt tgaatagttt ttgacgactt
     1381 gtgctcccga atgaatcaga agcttctact aattctttag gaacttgtcg aatccctaaa
     1441 ttcgtaaaac gaacggttgg tggcagagca aagatgactg aggcaaatac ccctggaacc
     1501 atcccgatac cgaaaaatgc aactgctgga atcaaataaa cgaatcctgg cattgtctgc
     1561 ataaagtcaa ggatcggttt gataatactt tgggctttac tgctttttgc catcaagatc
     1621 cctaatggta ccccaatgat gatcgagatc acactagata aaagaacaag tgtgacagta
     1681 ttcatcaaat cattccatag accttggtta taaataaacc atagaccgat taatgtgaac
     1741 aacgtaagtc ctggacgttt cttagaaata aagaaagctg ctaccgtcaa taacaagata
     1801 aacaaaggag ctggaatcac taacagtaaa tttgtaatcc ccgacatcac tgcctgtcca
     1861 atcgtttgta aaaatgaaaa taggccagaa aaagttgtcg taaaccattc tgtgatattt
     1921 tctacccagt ttgccactgg taattgatag ttcaatagat tattcatgtt cactcacctc
     1981 gcttgtttct gctaatgctt caatcacact acctcggatg acgacacctt ttaattttcc
     2041 atcctctaca actgcaagtg gagtcggtga gtcataaata atattgaaaa tatcacttac
     2101 caccatgtct ttcccaatca ccgtcacatt ttcatctagg aagtcgatca atggacgttt
     2161 ttgcttccta gcttctaacg cttgatctgc agtgatgatc cctttcaggt gtctctttct
     2221 gtcaacagcc atcagcatgc tgacttcttc attacgcatc cttgtcaatg caacgtttgg
     2281 tccatcagat tcgatattcg tcgtcaatgc aggtaccatg atgttttgag ccgtaagcac
     2341 tttggaacga tcgacttctt ccacaaattc acgtacataa tcatttgctg gattggtcaa
     2401 tatttcttca cctgttccga tctgcatgat ttcgccgtct ttcatcaagg cgattctgtc
     2461 cccaatccgt aaagcttcat tcaaatcatg ggtaatgaaa ataatcgttt tttgaacatt
     2521 ggcttgcagg tccagtaatt cgtcttgcat ttctcgccgg atcagcggat caagagccga
     2581 gaacgcttcg tccatcagca agatctcagg atcatttgct aaagctctgg ctaggccaac
     2641 ccgttgttgc atcccaccag ataactgact aggatattgg tctttaaatg aaagcaaact
     2701 agaattttct aatgcttttt ctgctttttc ttgtcgttct tctttaggta caccacggac
     2761 ttctaacccg tattctgtat tttccaagat cgttcgatgt ggaaacagac cgaagttttg
     2821 gaaaaccata ttgattttat gacgacggac ttctcgcaat tcttctttgc ttaacttcga
     2881 tacatcttct ccatcaatat agatactgcc cgaagttggc tcaatcagac gattcaatag
     2941 acggatcaat gtggatttcc cactcccgga aagccccatg atcacgaaga tttctccttc
     3001 ttttacgtca aaattcacgt catacacacc aaccgttgca ccggttttct ttaaaatctc
     3061 cgttttcggc tggtgatcat tcatcatatc taaagcggct tgtgtctttt taccaaatat
     3121 ctttgttaaa tgttttactt ctacttttac caaacaaatt ctcctttttt atgtaaattc
     3181 atttttcttt ctgaaaaaag tgaccacact atgttaacat catgtagtca ctttgtcaaa
     3241 aatgaaagaa atttgcttgt ccaaactgaa aaaaacttgg ctaaatcttt gtaaataaaa
     3301 ggtttagcca agttaatatt tttttaatat ttcacacaat cattctgcaa gctgatgctc
     3361 cgggtaaaaa aagtttgtca tacgctgtag tgtatattca tttccaataa aatgaacgat
     3421 gtcccctgcc gtaaatttag cataggggcc aggtgaaaca agcagttctt cattatgaga
     3481 aattgcgata actgtggcgc cagtcgcctg ccagatattc aactcgctaa ttgaacggtc
     3541 cagatgcatt gctttttctg ataaagataa ttcaaacgga agtaaaggat tcatcttatg
     3601 tattcgcttc gtttgattga ccagctgatc caataattga gaaaaatgat tcagctcatc
     3661 ttgttgcttc tccacacttg ccatgatttc tgcttttgta tcttggatgg attgtacatc
     3721 ttgatattgc aataggaaat cttttgcttt ttccttcgaa gcaacaaatg ccccacttcc
     3781 atgccgtact tccatgattc caagatctac cagcacattg atagccttgc gggtagtctc
     3841 aggagaagca ttgtacgtac tggcaagtgt tgagcgggca tggatcttct cacccacttt
     3901 atatcggttt tcaacgatac gttcggctaa atcaacagca atctgctggt agcgcgggcg
     3961 gacgattttt ttctttccag acattttctt tcctctttct ttttttactt tccctcatct
     4021 tactatgctc tagcaaaata agcaaaaaag gggagaatag cctcttgcaa cgactgatca
     4081 tcgcttgatc tgttcctttt ttactgggca agctgtttga cagtcatcac aattcttacc
     4141 ggattttaaa cgagtataga caattgcgcc tgcacttcca aagattaaca cacttaataa
     4201 gaatgtcgcc atcctattcc tctccttcta atgacaatat agtataaaac tggttcttgg
     4261 attttggctg acggaacaaa acataaacga gtcctatcat cagtaaaata gcaagatacg
     4321 tccctaacgc tacctgtcca ccctccaata agtgtcccaa ctgataaatc acaaaactaa
     4381 ctaaataagc taaaccgcat tgatagccta tggcgatcca agtccatttc ttggcattca
     4441 tttcccgatg gatagcccca attgccgcaa aacaaggcgc acaaagtaaa ttgaaggcta
     4501 acaatgaata agctgctgcg ggagtatatg tgtgttgtag cacttgccag atttctctgc
     4561 cattttcgga tacttccgca tgaccaaaca aaataccaaa tgtgccaata atattttctt
     4621 tagcgaccaa tcctgtaatc gctgcgactg ttccttgcca gtttccccaa ccaagcggct
     4681 gaaaaacaac tgccaggatt cgcccgaaaa aggctaagat actttggtct tcagaaacag
     4741 gttgagcata aaaattataa gaagagctga accagatcac gatactagaa acaaagataa
     4801 tcgttcctgc ttttttaaca aaagacttgc tgcggtcata ggtttgtctt aggaccgctg
     4861 accattgagg aaaatgatat gcgggcaatt ccatgataaa aggagctgta tctccagcaa
     4921 acaaacgtgt ttttttcaag ctgattccag agaaaataat tgcagtcata ccaagaaaat
     4981 aggcagaagg agctacccaa gattgatgag gaaaaaaggc ccctgctact aaggcaataa
     5041 tcggcaactt tgcagaacaa ggcatgaagg ttgtgaccat gatcgtcaaa cgacgatctt
     5101 gctcgttttc aatcgtgcga ctggccatga cgcctggcac tccacatcct gtagcaatca
     5161 gcataggaat gaaggatttt cctgaaagac cgaatttcct gaaaagtcga tccatgacaa
     5221 aagcgatcct cgccatatat ccgcagtctt ccagaaagct caagcataaa aacaatataa
     5281 tcaattgcgg tagaaatcct aacactgctc ccacgcctgc gatgatgccg ttcaagatca
     5341 gctcctgcat ccatcccgct acttgccatt cagctaacaa tctgccaaca tgttctggta
     5401 accatgtacc aaacaatgtg tcattgatcc aatctgtccc cattgttcct actgtttgaa
     5461 tagccagata atagatcagc cacatcacaa atgcgaagat cggcaaggct aaccaacgat
     5521 tcgttgcaat ctgatcgatt ttgtcactca tcgataattg gaaagaatca tttgacacaa
     5581 cacagagtgt acatagttgg gtaataaatt cataacgttc attgaccaga atcgtctcac
     5641 tatcgtcgtc caatagtttc tctgttaact ggatcagttg ctcgatctct ttttgctgag
     5701 aaactgctat ctcgtaatcc tctcttacct tctgatctcg ttcaaaatat ttcattgcat
     5761 accagcgttt cttgccgctt tgttctccta gtacatcttc aatttcatgt aaagctgctt
     5821 ctaaacgtgg atcatatgta cagggaacag gaatagaagg ccgctttttt gcagactcct
     5881 gaatcttttc gattagtttt tcgattcctt gctttttcaa tacgctgatt ggtaaaactg
     5941 gtacaccaag accataggaa agcttttcta gattgatttt ttttccttct tttttgatga
     6001 catccatcat attcagacca atgatcatag gaatgtctaa atccaataac tgcgtagtta
     6061 aataaagatt tcgttctaaa ttggtagcat caataatgtt gatgatcagt tcaggctgtt
     6121 cctgaaccaa ataatctctt gcaatcactt cttctggtgt ataaggagac aaagaataga
     6181 tgccgggtag gtcctgaata tgaatgttcg gatttttttt caacgatcct tctttttttt
     6241 ctactgttac gcccggccaa ttccctacat attgattcga accagtcaag agattgaaca
     6301 agctagtttt cccgctgttt ggatttccga ctaacgcgat attcattctt tttcctccct
     6361 ttgtatcaag attagttcgg cctctgactt tcgtaacgtc agttcatacc ctcgcactgt
     6421 cagttcgatt ggatctccca gaggggctag ttttcggata actattttta catttcgcgt
     6481 caatcccata tccattaacc gtcgcttcac tgcgccttgt ccgtggattg ctacgacgat
     6541 cccagtctct cctgctttta attgatcaag agaagtccag ctttcagagg aagcagtctt
     6601 tttattgaca aagatttgac ctagtatttc ccgacttact gccactcgat gactttttac
     6661 cagtaaaatg gcattgttgc ctgataaatt gacgacgacg attttacttc cggcaacgat
     6721 cccgatattt tgcaaatggc gttttgtttt ttcttctgtt tcgattcgtt cgacttgata
     6781 aacgaaattc tgctttgcct gcgctaaggt ctgcatattg attcctctct tgtgaaataa
     6841 atcatgtatt aaaatcattc attatatgtt ctaattttct tacgtgttac gcaaataaat
     6901 gagaatcatt ctcacataga atactctcgg taattgagaa tgtcaattat acttgtgata
     6961 ttatgagcga atctttagta ttgatagata aaaatgagaa aaaaatattt cttcatagtt
     7021 ttccacaatt ttttctctat taaatttgct attttcccac acgatacttg ttttcagctc
     7081 ttttttcaaa aacacaaaac ttagtattcc acagtttcca caacacaata tgtagtgttt
     7141 gttgtttgac ttttttacta agagttttat actatctatt atagataata agaatgtgtt
     7201 agaggaggca ctcaccatga acgtaaaaat cttttctaaa aataattgta tgcaatgtaa
     7261 gatggtcaaa cggtttttaa cagaaaatca aattgctttt gaagaagtca atatcgatga
     7321 acagcctgaa gcagtagact ggttgaaagc acaaggattc caaagtgttc ctgtcattac
     7381 ttctgatgca gaaacagtta tcggattccg tcctgatcag ttgcgtaaac ttgccagcta
     7441 acatgactaa aagaatcaaa acgtgataca tggatttgaa gggcttgaga cagcaccagt
     7501 ttttgtcgca aacttccaaa tcctttttta aagcttaaaa acaatgcaat gatttgcaat
     7561 caagcatcat caaatacgaa atgaaggttg ggcgtacgta tgagtcttaa aaatttacaa
     7621 gatgtcagtt atttcaagct gaataatgaa atcaatcggc cagtcaatgg tcaaatccct
     7681 ttgaataagg ataaagaagc tttagttgcc ttttttgagg aaaatgtcaa accaaatacg
     7741 atgacttttc catctattat ggaaaaaatc aattacttga tcgatcaaga atatatagaa
     7801 gcagaattca ttcaattgta ttcaccatca tttattgaaa agctatatca atttttggac
     7861 gagcaacatt tttctttcaa atcttttatg gctgcttaca aattttattc tcaatatgcg
     7921 ttgaaaacca acgacggttc cgcttatctg gaaacttatg aagatcgagt agccttcaat
     7981 gcgctttact ttgcgaacgg ggatgaagaa ttagcacttg ctcttgcaga cgaaatgatc
     8041 catcaacgat atcagccagc tacaccttct ttcttgaatg ctggacgtaa acgtcggggc
     8101 gaattggtgt cttgtttctt gactcaagtc actgacgata tgaactctat cgggcgctca
     8161 atcaactctg ctttgcaatt gtcccgtatt ggtggcggcg tagggattac tttatcaaat
     8221 cttcgtgaag ccggcgctcc tatcaaagga tatgagggtg ctgcaagtgg tgtcgttcca
     8281 gtcatgaaat tattcgaaga tagtttcagc tattctaatc aattaggtca gcgtcaagga
     8341 gctggcgtgg tttatttgaa cgttttccat ccagacatta ttgctttcct ttcagcaaaa
     8401 aaagaaaatg cagatgaaaa gattcgcgta aaaactcttt ctcttggcgt gatcgtacct
     8461 gataaatttt atgaattgac acgtaaaaac gaagatatgt atttatttag tccatatagt
     8521 gtcgaaaaag aatatggtgt cccattctct tatgtaaata ttacagctga gtatgatcat
     8581 ctagttgcaa atccaaacat tcgcaaacaa aaaatcaaag cgcgtgatct tgaaaatgaa
     8641 atctcaaaat tacaacaaga atctggctat ccttacgtaa tcaatattga tacagcgaac
     8701 cgcgagaatc caatcgatgg caagatcatc atgagtaatt tatgttcaga gattttacaa
     8761 gttcaaacgc cttctgtgat taacggcaaa caggaatatg aagtattagg aacagatatc
     8821 tcatgtaact taggctcgac aaatatcgtg aatttgatgc aaagccctaa ctttggtcaa
     8881 tctgtgcgta cgatgactcg tgcattgaca tttattacag atgcatctga tatcgatgtc
     8941 gtaccaccga tccaaaacgg aaataagctg aatcatacga ttggattagg tgccatgggc
     9001 ttacacactt atcttgcaaa agaacaaatg gaatatggct cagaggattc attagacttt
     9061 acaaatattt acttcatgct gttgaattat tggacacttg ttgaaagcaa caatatcgct
     9121 cgtgaaagaa aacaagtttt ccataatttt gaaaaatcag cttatgctga cggcacttat
     9181 ttcgaaaaat acgtaactgg agaattccag ccaaaatcag aaaaagtcaa agcattgttt
     9241 gaaggcattt ttatcccaac tgctgaagat tggaatgctt tgaaacaagc agtaatgaaa
     9301 gatggactgt atcatcaaaa tcgcttggca gtagcaccaa atggctcaat ttcatatatc
     9361 aacgatacga gtgcaagtat tcatcctatc actcgcatga tcgaagaacg ccaagagaag
     9421 aaaatcggta aaatttatta cccagcgcct tatctatcca atgagacgat tccgtattac
     9481 acttctgctt atgatatgga catgcgtaaa gtaatcgatg tttatgcaac ggcacaacaa
     9541 cacgtcgatc aaggcatgag tttgacttta ttcatgcgtt ctgaaatccc agaaggcatt
     9601 tatgaatgga agacaacaac gaagcaaacg acacgtgatt tgaatatttt aagacattac
     9661 gctttccaca aaggaatcaa atccatttat tacgtacgta cctttactga tgatgcagaa
     9721 gaaatcggaa gcaatcaatg tgaaagctgc gtgatctaat atcatagata gattcgctta
     9781 ttcatatttt aattaaagga gcattctcat gtcagaaaca tattatgaag caataaactg
     9841 gaatgaaatt gaagatatca ttgataaatc cacttgggag aagctgacag agcaattttg
     9901 gctagataca cgtatcccac tttcaaatga cctagatgat tggcgtacat tgtctcaatt
     9961 ggaaaaagat acagtaggac atgtatttgg cggtttgaca ctgctagata ctgtccaatc
    10021 tgaaagtggc atggatcagt tgcgtaaaga tgtccgcact ccccatgaag aagccgtttt
    10081 gaacaatatt caattcatgg aatccgttca tgccaaaagt tattcttcta tttttagtac
    10141 actgaatacg aaaaaagaaa tcgaagaaat ttttgactgg acaaatacga atccttatct
    10201 gcaaaaaaaa gctgagcgta tcaatgaaat ctacaaacac ggtacaccgc tagaaaagaa
    10261 aattgccagc gtctttttag aaacattctt gttctattca ggtttttata ctcctcttta
    10321 ttatttagga aacaacaaat tggctaacgt agcagagatc atcaaactaa tcatccgaga
    10381 tgaatctgta catggaacct acatcggtta caaattccaa ttaggattca acgagttacc
    10441 tgaagcagaa caagaaacgt taaaagactg gatgtacaat cttctttatg agctttacga
    10501 aaacgaagaa cgttatacag aagaattata cgatccaatc ggctggacag aagaagtcaa
    10561 gacattccta cgctataatg ccaacaaagc attgatgaac ctagggatgg atccactctt
    10621 tccagatact gccaatgatg tcaacccaat cgtaatgaac gggatttcaa caggcacaag
    10681 caaccatgac ttcttctcac aagtcggaaa cggctatttg ttaggtcatg tagaagcgat
    10741 gaaagacgac gattatttga ttggattgga ataaggattt attttaattc aaaaattttt
    10801 cgaaaaataa aaagagttta gcgtacagat attttaaatg tacactgaac tcttttttat
    10861 gcttattcac ttgtttcttc cagcactttt ggactgtcta acttttccga aacatgttcc
    10921 attcttctca ataaatagat tttccggata tttaatatta tcgtcttaat cactgcatag
    10981 acaggtatgc aaatcagcat gcctaatatt cctgcgacac ttcctgctcc aatcaatacc
    11041 aagatgatcg tcaaaggatg gatattcatt gatttcccaa ataacaacgg cttgatcaaa
    11101 tttccatcta attgttggat cactaaaatc gacaacgcca tatacaaagc ttgaaatgga
    11161 gagataaaca aaccgacaat gattgctggt accgctccaa taaaagggcc gacatacggg
    11221 ataatattcg tgattccgca aatgaccgca agcaacaagc tataaggctg atcgaatatt
    11281 ctcattgcca aaaagctcat tacaccaata atcaaagcat ctaagatcgt actgctgata
    11341 taggcagata atgtttcgtt taattctttt accgtttggc gtaactcttt acgaatcgct
    11401 ttcggaaaga agtgagacaa cgcatcaata aatttgtgcc catccttgaa cataaatagc
    11461 aaaataaatg gaacggtaaa taacaaaata aaaaattgca tcataaatga aatgattttt
    11521 gagacacttg cagataaact gactaatatg atattgaaca aattactaag agataaattt
    11581 gctgatgcta attgttcttc caaattgaaa tttttaaact cttcccgctg cgccaattca
    11641 tttaaccatt tgcttgtttc atctgcatac attggcaagc tctgggtcaa ttgaattgtc
    11701 tgttccatta actgaggcac tacattcatg acagttaaaa caactaaaat gattacgacc
    11761 aaaaacgtca ataaaaaacc gaacactctc gggatccgat gcttttcgag aaaaacaacg
    11821 attggatcga acatataata gataaaacca gcaacaagca atggtaaaaa aattgccgaa
    11881 agcattttca atattggact aaaaatattc ggcatttgta aaataaagta cacgccgatg
    11941 atcgtgagta ctagctccac tgtccagaaa aataattttg attgtttaaa acgtgattgc
    12001 atacatctcc tccaaggtcc tttgatactt ctattctatc atttaacaaa gagttacgcg
    12061 ctgagatttt catttgctta aaaaaaacaa aaaaaataaa catattttaa tgagtagacg
    12121 agtacttaaa caaaaacacg gtttggagta gtgacaaaaa ttatactgca gtcccttttt
    12181 ccaaataaac ggtgttcaaa agcttgcatc ttcgtaataa gcccagccaa acgaaaaata
    12241 taaggaacta tttttatttg gctgggctta ttacttgatg taggacatct attttacaac
    12301 ctcagatcat ttattgcgca atactgacta aatgcaactt tgtgatattt ttgtgtacgg
    12361 acaactcctc gatcacatcg gcataagtga gcccttttgg aagatcaatc gtatagatat
    12421 ttttgtacac tcggtagtca tcaacgaatt ttacgtcaaa attcacatct tcgattacga
    12481 tatttttctc ttcaaaataa tcggtaagaa aatctttggt ttcttttcta tggataaatt
    12541 ggatttctaa ctttttagtc gtaggtacat ggatcacgcg tttcactact gtgagtgcaa
    12601 tagcaatccc gataaaactg atgatcgcaa tggaatagta acccatgccg atggcgatcc
    12661 ctaatccagc aattgcccat aaagaagcgg cagtcgtaag tcccgtcacc gattgtttcg
    12721 tgacgatgat cgttcccgcg cctaagaaac cgacaccact caccacttga gcaatcagcc
    12781 ttgcttcatc tgaacgaatc gttccagcaa gctgtggata ctctaaagca tctcttaatg
    12841 caccactagc gatctcaact tggatcaatg caatgataca tgctcccata caaactaaaa
    12901 tatgcgtacg catacctgct ggacgatttt tatattgtcg ttcaaaaccg attgctccgc
    12961 caaatagcat agcgatccct aaacgtaaaa taatctctgg aattgtcaat accatatcat
    13021 tcatctaaac accctccagt ccaatatatc atgtctgaaa ggagcagaca aaaaaagtgc
    13081 aatttttacc tcttacgaaa aaaggactgt gacaaaattt ttgtcatagt cctttttatg
    13141 aataaacggt gttcaatcag ctgaccctcg gtattactca acaaacggca aaatatgggg
    13201 agctattttt cctttgtctt ctctaatact caggtcaaaa cgctgatttc acaacctctt
    13261 tagcctacta ttgaaaatta caacttactt gctgcttctt catcgatgat cacaacaaca
    13321 ttgtcgtgat tttgcaatac acttgctggc aaatgattcg ttacaggtcc ttcaatcatc
    13381 cctttgattg catcagcttt gctttcgcca aatgccatca agacgatttc tttacttttc
    13441 ataattgaac cgattcccat tgaaacagct tttgttggta catcttctgc tttatcgaag
    13501 aatcgtttgt ttgcattgat tgttgattct gttaagtcaa ctactgaagt cgttccgtca
    13561 aatgaagcac caggttcatt gaaaccgata tgtccatttt gcccgattcc taagatttgg
    13621 atatcgattg gatgtgcatc gatgatttga tcatactttt tgcatgctgc ttccagatct
    13681 tctgcttttc catctggtac aaacgtttct ttgaatggtt ttttatcaaa taattggaca
    13741 tccatgaaat gacggtagct ttgttcatct gaaccgccta gtccaacgta ctcatctaag
    13801 tttacagaag tcatttcact gaaatccaaa tcgcttgaag tcatttcttt atataaagta
    13861 actggtgtgc ttcctgttgc caatcctaat acttttgcac ctttgttcat accttctttg
    13921 atgatttcaa atgctttttt tccgccttct tcagcgtttt ttacacgaat gatttccata
    13981 gtgattgct
//
LOCUS       DAKBVG010000061        13956 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000061 DAKBVG010000000
VERSION     DAKBVG010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.061"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(99..1229)
                     /locus_tag="OUD09_001252"
     CDS             complement(99..1229)
                     /locus_tag="OUD09_001252"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCT9692531.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="OUD09_001253"
     CDS             complement(1878..3080)
                     /locus_tag="OUD09_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCT9692532.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="OUD09_001254"
     CDS             complement(3140..3343)
                     /locus_tag="OUD09_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HCT9692533.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="OUD09_001255"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="OUD09_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4880..5692
                     /locus_tag="OUD09_001256"
     CDS             4880..5692
                     /locus_tag="OUD09_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCT9692534.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6716..6913)
                     /locus_tag="OUD09_001257"
     CDS             complement(6716..6913)
                     /locus_tag="OUD09_001257"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HCT9692535.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            complement(7059..7937)
                     /locus_tag="OUD09_001258"
     CDS             complement(7059..7937)
                     /locus_tag="OUD09_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCT9692536.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(7976..9094)
                     /locus_tag="OUD09_001259"
     CDS             complement(7976..9094)
                     /locus_tag="OUD09_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HCT9692537.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(9091..11190)
                     /locus_tag="OUD09_001260"
     CDS             complement(9091..11190)
                     /locus_tag="OUD09_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HCT9692538.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(11241..12062)
                     /locus_tag="OUD09_001261"
     CDS             complement(11241..12062)
                     /locus_tag="OUD09_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCT9692539.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(12059..12868)
                     /locus_tag="OUD09_001262"
     CDS             complement(12059..12868)
                     /locus_tag="OUD09_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCT9692540.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(12889..13383)
                     /locus_tag="OUD09_001263"
     CDS             complement(12889..13383)
                     /locus_tag="OUD09_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCT9692541.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(13395..13883)
                     /locus_tag="OUD09_001264"
     CDS             complement(13395..13883)
                     /locus_tag="OUD09_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCT9692542.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
BASE COUNT         4245 a         3022 c         2253 g         4436 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagactac aataatattt ttctaactta tcgccttcaa agaaatagtt
     6421 tcttattttt cactaaaata aaaaatttat tgcaaaaaaa atagaagtga caaaattatc
     6481 aatcatttca aggtaatata actacctgag tttattatcg attaaaatgc taactacatt
     6541 tgcaagagaa gcgagagtta aaggtaaata tagaatttct acttttttag caatagaaat
     6601 aggaataaaa tatttaaaat gtatagaaac tttctttttt tttcttgcta tctttttata
     6661 atcacaactc tctataatag aattacgatt tattttatac ggctgaaaaa atctatcacc
     6721 aaaaaaattc aaacattaag ccaaggatta tgaagtcaac atttcgaaaa atgaacatac
     6781 attggtaagc aactataact attaccacag acataaagta acacaatata ttactattta
     6841 agtgtacgcc accagtgaat atcctcaagg tatacagcgc acaacaaaaa gaaaaaagga
     6901 aacgcagaag catttccttt cagtgattta atgaaaacaa cacgatttga cttagagcct
     6961 ttttatttga attcattttc gctttatcca acttcatttg atcatttgat tactcggtta
     7021 aatacttttg acatgagtat caatgaaaca ttcattaatt atggttgctt atttcttacc
     7081 gcttttctgg ccaatgctaa tccgccgatt attccggcat tatcacctaa ttcgggcgta
     7141 ataatgtagc tatcaattgg tggtatttca acatatccat ttataagctc aacaaattta
     7201 tcctgaactt ttttcttcat gtgttcttgt ttcattacac caccgccaaa aataattcta
     7261 tctggcgaaa acatcagtgt tgtattgtat gcacattggg caaggtaaaa ggcttcaatc
     7321 tgccaaaacg aatcgtcagc caataaattc tggcctttta cacccaaccg tttttcaatc
     7381 gctggtcctg ctgccatccc ctctaaacaa tcttggtgga agggacagtt tcctgaaaaa
     7441 ttgtcctttg gatgtcgctt gatcaacata tgccccattt ccggatgact gaacccttcc
     7501 acgaaagtcc cattgaacaa ggcaccacct ccgatacctg ttcccacagt gaagtatgca
     7561 acacctgcta accctttgcc agaaccagca acatattcac catagcaagc cgcattgaca
     7621 tctgttgtcc attcaatcgg aatattgaaa tattgcttta gcgttcctac aaaattgaaa
     7681 ttttgccaag caagttttgg tgttgatgtg atatgaccga aactcgttga tttgcattga
     7741 atatcaattg gaccaaagca gccgaccccg atgcctgcca attgttcctt aaaaggttta
     7801 aaaaaatcaa tgacctgctt cattgtttca actggagttg ttgtgggaaa actgactctt
     7861 tccaaaatcg ttaattcctc agtaccgact gcacagacaa atttagtccc tccagcttca
     7921 attgccccat atatcatcat aggttcctcc cttcgaatat gtgaaaaaca cttcattaac
     7981 gcggcaagcg ttctcgatca gtgaaatgac gtttagtatt tcctgaagct tctgttgaat
     8041 accagtaagc gacactagca acatcgtctt gccgctcaaa taagcgaaca tcatcgttgc
     8101 cgatttgttg taaagtgacc gataaatctt cggtaaaaca gatggggtct tgaagatgcc
     8161 aacgataaag accatgtcgt ggcattgcgt cgtcaccaaa ggcatgaacg gcattcaatt
     8221 ttcccgtttc aaaacgatct cttgtggcat ctctatttga ttggtaagga taccctaaaa
     8281 aggctgttga atagtttttc gcatgtggac gtccgaactc atcgtactca tgaaaggccc
     8341 acgccccgcc aaaataatct tctgaacctg ttgaggttac ggtcggatac tcactatccc
     8401 catcaatgta gaatttaaac tcgccttcgc cccaccaata acgttctagg caggtaagcg
     8461 caaggtaagt acccacgtaa tagccttgac cctttaatcg atcaattaac ttataatcct
     8521 cctgtaattt ggtaatgcgt tcccgatgcc aatttgcgtg aaaatatagg ctattttctt
     8581 ccaattcgtc caccaaagcg taatttatcg tataaaaaaa tgaggacata tccttcggat
     8641 gtaaattctc gatcgtaatt ttagcggatg tttggaaagg catttcgaaa taggaattaa
     8701 agccccctgt aggattaacc actatcggca acgagttcac ctcgcaacgc tctccaaacc
     8761 cattacaaaa gaaatcccct aaaggacttt caacagcagg gctatccgac ccatcccaat
     8821 aaattcttaa gattaaatca cgtaaaacaa aactgccaaa ttctgttcga tcacgaatcg
     8881 tcatccacat atgacgaatt attcccgttc cagtaatatc cgcaatcaca gtttcttcac
     8941 cagaagtcag cggaattgcg ccacgacctt tgcggcttgg tcctagctcg cttggcaaca
     9001 tcgcagcttt acctttctcg cctgttggat tttcgggagt gattgctcga ctctggattt
     9061 ttttaaattg ggtaatattt tttagtatac tcatcttttt ctcctttgaa tgtgaatcgt
     9121 ttttcccttt tctacgtgaa gaattttttt cgtatcactt attttcaatg gcttttctga
     9181 caaatttgaa atactcaact cggattgcgt acattcaatc gaaatgaagg cttgaccgaa
     9241 cttcaaattc ttccaagaaa acgttaaatt atcaacagga tcaatttgca atactccttt
     9301 tttgaatttc aatccgaaat attgactgat cattaggctg acaaaaagac ccgatgccca
     9361 gccgcatttg ccgcagccaa agtctcgcac gacttctcct tgttgagcct ctaagcgata
     9421 cggccaccac caccacgaac cgtctaaatc tgccaaacaa attagttctt ctaatcgggt
     9481 tacgaaagct tgagggttgt gcagctcgct cattaaaaca gtgataaagc caggaaaagt
     9541 ggcgccggat tctaatcccc aagtgatgcc tttgatttcc tcgctatagg ttggattgaa
     9601 agaactagca gaaaaggcca tggtgttctg atataaatga tctttattat ctaaaaattg
     9661 ataaaaaggc atcaacgtag tgtctgattc ttctccatca tgcatcaata aattaatttt
     9721 tccatccaca atcacatcac ttaaaaagat taacccttgc tcaagaatcg gtttttgata
     9781 gtgcttcact gaataggttg ttttctcctc atcaccgatt ccctccaaaa attgctcacc
     9841 aaaaggacca tcgatagtca tttgtttaca aattgattcc tttagtttac aggcaatgtt
     9901 tttgtatcgc ttgctaagac tcagttgttc tagggcttca aaaatttctg caagtccaat
     9961 acaggccttc caaagacaaa gattgctgcc ggtatgatac tttccaagtg caaaggcgtc
    10021 tgataaccaa acagatggaa aaagcattgg ttcccctgac cgacgttgag ctaatatgtc
    10081 ctcaataatt cttgaagcat tcagtagaac ctgcggacac cgctcaaaga attttgtatc
    10141 cccagtatgt tcaaaataaa gactagccag taacgcactg gataacgaat tactcagaga
    10201 atgattgata cctcctgtaa attttgaacc aggaaactta acgccatatt gatcaaacca
    10261 tagaaccgtt ttttgacata actccggttc aaacatggtg aatggcaatg atgcgtaata
    10321 catatcctta ttccaaattc gattagtagc aggatagctg ccccaattgc tgccgacgac
    10381 ctgtccctgt cgattcatac cgaaactaga aaagctttga taaagtgcac gacgatagag
    10441 atggacaact ctatcgtttg gcattgacag ctgcccaaat agctgagcaa aatagttcaa
    10501 tgtttccttc agccattttt cgatatcaga ttcctgaaaa gtcattactt cttcaaaagc
    10561 atttgggtcc acaaaagcaa tggcgaagct tgccacttcc tgttgtgcga tcgtgtcctt
    10621 atttcctttt tgagaaatca acacattttg ctgatccgaa tacttctctt gatacgaagg
    10681 cacatctttc actgaaactt gaaggctttc gtgtgtttca ttcacaatgt aaacctgttg
    10741 aacaagcata cttaaacgct tattagccag tatgggagca aaggatacta caatgacctt
    10801 aaacgtcgaa taatgatgaa tatatctagg caaacagtct tgaatcacat cgacttcaat
    10861 acgatgatcc gattctgaca aatggtagac aatcccgttt acttcaacct ttaaggataa
    10921 cgaattggtt tgagagagct gtttattgac ccaaacacct ggtttttcct cagttagtaa
    10981 attttctcga taaaaggtca acgtttgaat cgtcccgcaa acatcaaggt ttgctaatgt
    11041 cttttgatta cttacatcga aggtcaatcc ctttacatca ggaatattcc gatagtagct
    11101 gtattcgttt gaagtagtca catttccgtc gtttaactca gcattaaaaa atgggtgttt
    11161 gggtgaataa attacttcat taatagtcat cttttccctc cttgctaaaa aaactgcagc
    11221 ctagtagctg cagcttccta ctatgcgatt ccaagtaatg acaataaaat tcccaagacg
    11281 accattccaa aaataatttt gatgacgccg acttttttat ctagtaattt gaaacagctc
    11341 aatgttacca ataacggtac aattccttta aaaatactat cgaggatttc ttggacattc
    11401 attagttctt cgcccttcat tgaaaagctc aaaacggtat tgaatttgac catactgatc
    11461 gtcattgcac ctaccatgat cagacccaag atactagcag cttttgtcag aatttcaatt
    11521 aacccgttcg catacagctt ttcaatatat ttagagccta atgaatagcc taaaacgccg
    11581 ccatagtatc gcatcaagta attaggtata ttgaacataa ctaaaaagat aattggtgcc
    11641 aaaatatttc cggcattggc taagttgatc gctagtcccg cggtaacgac acgccaaact
    11701 ccccagaaca atgaatcgcc aattccagcc aatggtccca tcaaactcgt tttgatcgcg
    11761 ttgatacttt ccacgtcaaa accagctttt tcagaatttt ccttctccat cgatgccgca
    11821 atccccatga caaaggacgc gcaaggaatg gtcgtcgaaa aatactgtgt atgacgttcc
    11881 agcgccgcag ctcgatcttc ttcacttttg tagaaatgat taatgaaggg catcatagaa
    11941 taggcgaaac caccgccgcc catcgtcacc ggattcaccg aagcatacat agtcatcgag
    12001 cgccaaaaca tcttccgaac caattttttc tcatctttag aaattccatc agctaatttc
    12061 attgaaagaa atcctcctct tcttggtcaa gcgttgcatt tacagcagca tcgctttgaa
    12121 ctgcatgaac agatatattt tttagttgta gatctctttg ggctataaca attgcaataa
    12181 tcgttccaac tgcggcaatt gcaatcaatg gtaactcgag atacgccata acaacggccc
    12241 caaagaaaaa ccacacagca atatttttac tccaaagcat ccgtaacagc atcgccatcc
    12301 caactgccgg cagcagattt ccagcgactg ttaatccgtt catgatgacc tctggaatat
    12361 tatccaagac gcttttgaca acatctgctc caaataaaac ggcaaagaat ggaataagag
    12421 cataaagcgc atagtggaca aaccacataa agaagtgcag attgatgatt cctttttgat
    12481 caccagattt tgcaaatttg aaaaatttag cagccatcgg tccacataca aaagaaaaaa
    12541 tcatattcat taactgtaat cctaatactg ctagtggaaa gacaatggga ataatcgcat
    12601 ccacgtttcc tcccattttg attgcgaacg ctgtagccag cccagcagcc aaagaaggct
    12661 ccgccgtact agagattccg atattgacga cacccataaa gatggcttct agtgacgccc
    12721 cgacaatcaa ccccgtctgc aaatccccca tcaacgctcc tgctaacgga gcaacaacaa
    12781 tcggacgact cagcatggac atcccgaaca tttcatgtcc agcaactcca ataaaaacgg
    12841 ccaaagcgac tacaaatgca tcatacataa tttttctcct tcctttttct aaaaatcact
    12901 caaaatagag atagctgaac gtttttccat cgccggtgta ggctgaaaaa cggtatctgg
    12961 ataaagttca actaattctt ttaagtcttg aatttctgac gtcgttaaca tcacttcctt
    13021 tgataaggtt ttcttatctt ccgatggcgg tgtcattttc ccagcattcc ccacgttcat
    13081 taattcgatt tccgttggga aagctttcgc aacttttacc gcatctttga cattagggac
    13141 taaaaccatc aagcgcatgt ccttcgcccg cggatctttt aacaaatgaa tcgcgtcatc
    13201 aactgtttta ataatgactt tgacattcga tggtgcagcc atcttcaatg tcatcttttg
    13261 aatatcgttg gtagccgcct catcgcttgc tactactaac ccggttaaat ttagactctt
    13321 tgtccaagtc atcgctactt gtccatgaat taatcgttca tcaattcgca ctgctttaat
    13381 catttttctt cctcctaaaa aaaatcttcc tcttcattta ctacatgtac ctcatcaaga
    13441 atggtcatct ttaattcctg ttggcattta tttaccagtt cctgaatatc ctcctttgtt
    13501 aattccttgg ttgccaatac tatttcgagc agaattggca gattaaagcc agacaaaata
    13561 gttacagcaa tctctttctc caaaaaggtt ttggtaatct tctgattgac acttccgcca
    13621 aataaatcgg taaaaacaaa aatctgatcc tgctcgttca cctggtttac aaatacattc
    13681 aactcctgtt caaaatcaca ctcatccaca taagcattaa tcaccgtaat ctcttgaacc
    13741 ttccccgtca gtaattgaat actgctaaga aagcctgaag ctaagtgacc gtgagtcgct
    13801 attagaattt tcttcatatg gttaattcct ttctttctgt tcacttttat ataagcaatt
    13861 cctgtgccaa cttttaaaaa cattggttta atcacgttaa ttcacattaa aaaaagtgtc
    13921 taacctaaaa aaaaacagat tagacacttt tttaga
//
LOCUS       DAKBVG010000062        13887 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000062 DAKBVG010000000
VERSION     DAKBVG010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13887
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.062"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(66..293)
                     /locus_tag="OUD09_001265"
     CDS             complement(66..293)
                     /locus_tag="OUD09_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HCT9692543.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(290..808)
                     /gene="msrA"
                     /locus_tag="OUD09_001266"
     CDS             complement(290..808)
                     /gene="msrA"
                     /locus_tag="OUD09_001266"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCT9692544.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(828..1541)
                     /locus_tag="OUD09_001267"
     CDS             complement(828..1541)
                     /locus_tag="OUD09_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HCT9692545.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(1544..2440)
                     /locus_tag="OUD09_001268"
     CDS             complement(1544..2440)
                     /locus_tag="OUD09_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HCT9692546.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(2598..3440)
                     /locus_tag="OUD09_001269"
     CDS             complement(2598..3440)
                     /locus_tag="OUD09_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HCT9692547.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEEILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            3572..4225
                     /locus_tag="OUD09_001270"
     CDS             3572..4225
                     /locus_tag="OUD09_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCT9692548.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(4289..4816)
                     /locus_tag="OUD09_001271"
     CDS             complement(4289..4816)
                     /locus_tag="OUD09_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCT9692549.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEIDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(4836..5783)
                     /locus_tag="OUD09_001272"
     CDS             complement(4836..5783)
                     /locus_tag="OUD09_001272"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCT9692550.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKEVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(5795..7681)
                     /locus_tag="OUD09_001273"
     CDS             complement(5795..7681)
                     /locus_tag="OUD09_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCT9692551.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSGDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            complement(7846..9918)
                     /locus_tag="OUD09_001274"
     CDS             complement(7846..9918)
                     /locus_tag="OUD09_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HCT9692552.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRHADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
     gene            complement(9931..10206)
                     /locus_tag="OUD09_001275"
     CDS             complement(9931..10206)
                     /locus_tag="OUD09_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9692553.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(10568..11023)
                     /locus_tag="OUD09_001276"
     CDS             complement(10568..11023)
                     /locus_tag="OUD09_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9692554.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(11177..12385)
                     /locus_tag="OUD09_001277"
     CDS             complement(11177..12385)
                     /locus_tag="OUD09_001277"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HCT9692555.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPSLKEEGEALLRFAELPSVPLKEESQVWTLLISQLGLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(12497..12829)
                     /locus_tag="OUD09_001278"
     CDS             complement(12497..12829)
                     /locus_tag="OUD09_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HCT9692556.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            12977..13852
                     /locus_tag="OUD09_001279"
     CDS             12977..13852
                     /locus_tag="OUD09_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCT9692557.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
BASE COUNT         3834 a         2868 c         2392 g         4793 t
ORIGIN      
        1 aataattctt tcaattaggc tattggacaa aaaacttttt tgatcataaa gtacaaaact
       61 atctcttata tgtcatgatt attttctaaa tatttttccc atgcttcgtc aaagatatcc
      121 atggacggca aatagtcgac attcagttcc agataattag aaatttcttc gtagctagag
      181 ctgtgcttag gaaattggat gtctttaccg gcttctgtag cgaaaacaga taaagcatca
      241 tgtgtgccac cgcctcgcag tgtcattaaa taatgaaaaa agcttcgatt cacccttcgt
      301 tcctccaatt ttcttcgata aaggcagccc ggcgtgcatg gtttctttta aaattttcag
      361 gatgcttttt ataatactct tgatggtctt cttctgctag ataaaatggt tgggctggtt
      421 cgatttttgt aacgattggc tgttcgaaac gtccactttc agctaactgt ttcttacttt
      481 tttcagcaat gatccgctgt tcttctgtca aataaaagat caccgggcga taattgtcac
      541 cgcgatcctc gaattgtccg aaagcgtcag ttggatcagt ttgctgccag taaagttcta
      601 ctaattgttc atatgaaatc aatgttggat catagataat ctccactgct tctgtatgtc
      661 cagtcgtttg gctttttact tgttcataag tcggatctgg tacatgccct ccagtatatc
      721 ctgacatcac tgtatggata ccaggaagcg tgtcgaaagg ctggatcata caccagaaac
      781 agcctcctgc aaatattgct cgttccatta tgattccccc tctaatacta ctcatccgat
      841 gattttttat tttcttttgt cttgctttca gtactgctgg tgctagtttt gttggtattt
      901 gagttgtttg aagcatcaga ggtatctttt ggcaaatata gactaaaccg aatgtcatca
      961 tttactaagt cgattttatc tgctttaata tacatcccgt tttgcattcg gaattgatcc
     1021 aacctaagca tgatagtcag atcatctgta ttgacttcga tccaatctgg taatttatag
     1081 tttctcttga tcatattcat cacatctttg attggcaaac tcaatgttcc gatagaaaga
     1141 cttctagctt ttagttggac atttccgttt tccatcacat acggatcaaa atataaatag
     1201 aaatcgatag gaaaacctag tacttcgaaa gtaccattga gtagtgcttc attttccaaa
     1261 taaaatttat attcaatatc actatctttt tgatactcac taaggaaaaa gctgatgatt
     1321 tgattgactt tttctttatt tgaattgatc gttaagacag gatctccttg tctttccgta
     1381 agagctggta tttctgggag ttctggttcc cgattagcaa aaatacggct gcctaaaaat
     1441 acggtcgaac caagtactac cccaagaagg ataaggaagg cgaatttcca gtaatttgtt
     1501 ttctttgttt tgtttgaaga tacgtcagat cttgttttca tgtttattga tcatcctcct
     1561 tctctagcca ttggttttgt gtctcgatca gctgatctct cactgcattt gccatcaatt
     1621 gataacctaa attatttgga tggaaatgat cttcttcata gagcacatta tttttgatgc
     1681 catttagatt ttctgtcgtg ctagttgttt gatctgttcc ttcttctgtg acacctactt
     1741 cctgattcaa tccttgataa agcaaatcat tgattgggat aaagtagcag ttcggcattt
     1801 tttttgtcac cgtttctgtt cctttgttcc aattgttaac aattgtctgc atgtcagtga
     1861 tctcaggaaa gttcaaataa aatggattgt agatgcctaa aatgtaaata ggtgcggttt
     1921 tattgaggga tcgaaattct tccaatagtt ctgtgacatt ttcctgatag tttttcagtg
     1981 gctttttaaa ggaattgacc gttaacccga ataaattatt ttggataact ttcatcaaat
     2041 cattaccgcc aacagtcagt gtgataaaat cagctgtttt gatattgttt cgaatttctt
     2101 cttttgtttt tactcgcttt aagatctggt cacttcgttc accatttaca ccatagtttt
     2161 ctgtttcaac cgcggtcaaa ttataacggt cttttagatc atttgcgaca aggggaacga
     2221 atcctccttg cttggtttga tctcctactc cttcagtcag cgaatctccg atagctatgt
     2281 agtgaagttt ttctttttta tttggatctt ctctcacagt agttactggt ttcattagtg
     2341 ggccagcttt cggtatagaa gcagctaaac ctgcatagat gatgatcgca attaataatg
     2401 ggaacaaaac gaggatcaga tgtcgtttcg ttaatttcat tttttcacct cttgtattcg
     2461 attggttcta atgttatcaa aaattttatc aaaaaggtag tctttatgaa aaaagaaaac
     2521 aattattttc ctaattttat aaagaaaaag agaataagct gcaagccttt caggctgagg
     2581 gcttatcctc ttttcgacta atctacataa tacatgattg caaacgcgtc tttaccagta
     2641 tgcgtagcga taaccggatt cgtatgcagt acagggatat gcatgtccgg gaataattct
     2701 tgcaaaccct ctttgaattc ttgcgccaat tcttttgcat ccgcatgtga aataccgatt
     2761 tgtcgcacat ttggcaattc tttcagttca tttttcaaat tattaaacca tttatcgaac
     2821 gttttctttc ctcgcccctt agttacaggg attaattcag aatgggcgaa attcatcacc
     2881 acgcgtatgt tcaaaacact agacagcaaa ccagttgttc gactgattct tccacctttt
     2941 accaagttat ctagtgtgga tattccgatg tataattttg tattgtcttg tacttttttg
     3001 acttcttcaa ggatttcctc tactgtttta ccttcttttg ctaattcagc tgctcgaatt
     3061 acctgaaaag ataatccttg gtcagtggta tcactgtcga taaccgttac tttcgttttt
     3121 gttagattac tggcttgtct tgctgcttct actgttccac tcaagccttt tgtcatatgg
     3181 atcgaaatca cttcactgcc gtcttctcct agctcatcat acatcgagat aaattctcca
     3241 atcggtggtt ggcttgtttt gggcagattt tttgcttttg ccatcatttc cataaatctt
     3301 tcgccgtcta gttggtcatc atcgggatat actgttccgt cgatcatgat ggatagcggc
     3361 atgacacgaa tgcctaaagc atcgcgtaca tttttttcca tcgtacatga agaatctgtt
     3421 acgattttta catttgtcat tattttctct ctctctttct ttaacaagat gccttattca
     3481 tgttaaacta catgtaagac aatttctatt tttagtataa cagacaaata atgatttttc
     3541 attaattaca cctagtaaag gagccgtttt catggaaaaa acgaaattta atcgaacgta
     3601 tgaaatatta aatgaagtgt ttaatgcagt cacccatgga attgggactg gattgagtat
     3661 cgcgggacta gtggttcttt tagtaaaagg tgcccacttg cattcctccc tccatattgt
     3721 ctcttatgct atctatggat cgatgatgat tcttttattt ttaacgtcta ctttgtttca
     3781 ttcattgatt tttacaaaag caaaaaaggt gttccaagta ttcgatcata gttcgatttt
     3841 tcttttgatt gcaggaagtt atactccttt ttgtttgctg agtatccgag gatggctagg
     3901 ttggacttta tttgtattaa tctggttgtt tgcgattctt ggcattatat ataaagcgtt
     3961 gacgttgcat aaacgggaga gagtatcaaa agtatctacg atcatctata tttttatggg
     4021 gtggctctgt gtcgtggctg ctgttcctct ttatcactct cttggaatga ctggtgttat
     4081 tttgatggta gcaggcgggc tttcttacac gataggcgca ttcttttata gcttgaattc
     4141 tatccgctat atgcatgttg tttggcattt gtttgttatg ttaggtgcag gttttatgta
     4201 cttctctgtt ttattcttta cttaataaaa aaaaataaag ctatggatgg gacagctcta
     4261 aataacagct agtctatcca tagctttttt atatcgtcaa ttcgcctgtc ccgcttttta
     4321 caggattctc attacgctga tacgtttcaa attgatgcgg ataacgattt ttttcatcta
     4381 tttctccttg actagtattg atcagtgacc aatcagacca gtcaacaggt ggaaaataag
     4441 catctccatc gaatacatga tggatgactg tacggtataa tatcgtacaa aatggcagaa
     4501 attcttgata aacagctgac cctccagcga tgaaaaagat accgtctgtt tctttttcca
     4561 gagcaagcac gtcttccaca ctatgcatga catgcacgcc ttctgcttta taggaagaat
     4621 ctctcgtcaa aacaatcgtc tgacgattcg gcaacggacg gctacccatc ccttcgaaag
     4681 tttttcgtcc catcactagt gtatttgctt ctgtcatttg tttgaagaat ttcagatcat
     4741 ttggcaaacg ccaaggaaga cggtcttctt ttccaatcaa tccgttttca tcctgtgccc
     4801 aaattgcagc taacattttc tcatcctttc tgattctaaa cagcgatagg agctttgatc
     4861 gccggatgtg ggtcatatcc ttctaattgg atatcttcca tctcaaaatc aaatactgac
     4921 tgcttttcct gattcaaaac taaagtcgga aatgcgcgta tttctcggct caattgttct
     4981 ttcatttgtt cgatatgatt gatataaaga tgagcgtctc ctaatgtgtg aacaaattcg
     5041 ccaaccgcta aacctgtttc atgtgcaatt aggtgagtca gcaacgcata acttgcaatg
     5101 ttgaacggaa cgcctagaaa cacatcccca ctgcgttgat acaattggca gcttagccgt
     5161 ccctcttgaa cataaaattg aaacatcgta tgacaaggag gcaaagccat gctaggaaca
     5221 tcttccggat tccatgcaga aacgatcaat cttctagaat caggagtctt tttgattgct
     5281 tcgatcactt ctttgatttg atcgataaag ccaccgtctt ttgtttccca gtgacgccat
     5341 tgatagccgt agatgtgtcc aagatcgccg tatgtttcag caaaggaatc atcggttagg
     5401 atcttctcgc aaaaaagctt gctctgcttt ttatattctt cattgaattc aggatctgtc
     5461 aaacatctat ggccaaaatc agtcatatct ggtccattat agtctggact tttgatgtag
     5521 cgttcaaatg cccattcatc ccaaatatgg tttttatttt gtaatagata gcggatattc
     5581 gtatctcctt ttaaaaacca aagcaattcg cttttgatca atccaaaagg tactcgtttc
     5641 gttgttaaca aaggaaaccc tttttgcaga tcaaaccgca tttggtatcc gaataagctt
     5701 aatgtgcctg tgcctgttct gtcttcttta cgatgccctt cttccaacaa ttttcttcct
     5761 aagtcaagat acgcttgttc catccttttt cctcctaatc ttctacccat tcactcaagt
     5821 attcccaccg tgccattttt tcttcaagtt gtgcttctgt ttcagatacg tcgttttgca
     5881 attcttgtaa tcgagtgaaa ttatctcctt gatgattcat ctcttcttgg agcaatgaga
     5941 ttttttcttc aagttctgca atctcatctt caatcgtttc ccattctttt tgttccatgt
     6001 aagataattt cttcttttca gcaggttcct tgctctgtat ttttggtttc gctttttcag
     6061 caggctgctg ggtctctttt tgcttcagta aataactgga catgtttccg taataaagtt
     6121 cttgttttcc gtttccttgg aatactaata acttatctgc taccttgtcc aagaaataac
     6181 ggtcatggga aacagtgatc acagcacctg gaaaacttct aaaataatct tccaaaatcg
     6241 tcaatgttgc tatgtccaaa tcattggtcg gttcatccaa taataaaaca ttaggttgct
     6301 ggatcagcag tttcaacaaa aataaccggc gtttttctcc gcctgaaagt ttgcctatca
     6361 gtgtcccatg cataaatctt ggaaataaaa atcgttcaag taattctgct actccgattt
     6421 gactgccatc tgctgttttt gcttcttcag cagcttcttg taagtaagca atcatccgtt
     6481 tatctggtgc catttcttca ttttcttgtg tataatacgc aagatgaacc gtttcaccga
     6541 tagacattgt accgctatcc aggggaattc tgccagctag aatattaagc aatgtcgatt
     6601 ttcccgcacc atttttacca gtaataccta gtctttcccg agattgaatc agtaaatcta
     6661 aatgttctag cagtgtttgg tcatttatac ggtaactgcc atctttgatt tccagtactt
     6721 ttttccctaa acgttgtgtc gctaggttca gctctaaatc atcttcttgg tttacttggt
     6781 acaagttctc ctttagatct tcgaaacggt tgatccgtgc ttgctgcttg gtgccacgtg
     6841 cctgtacgcc agccctcatc caagctaatt cctgtttata aagccgcttg cgcttttctt
     6901 cttgttctac ttctacacga tcacgttctg ctttttctaa gacgtaagct tcataatttc
     6961 ctttatactc gtataatttt ccaaatgaaa gttcgaagat tcggtttgct actctatcta
     7021 gaaaataacg atcgtgggta accatcaata gtgcgccacg gtattgtttc aaatagtttt
     7081 ccaaccactc gatcgcttca taatctaaat ggtttgttgg ttcgtctagt aatagtaaat
     7141 cgggttcatc aatcaacact tgcgccagac taactcgttt tttctgacca cctgataaag
     7201 tactgatttt tttatctaat tcactgattc ctaatttttg caaaataatc tttgcgttgg
     7261 tatctgtagt ccaagcatct tctttattca tttgttcttc tgctttagca taacgttttt
     7321 gcacatcagg gtcttctccg ttttccccta gttcgatcaa tgcttcctca tatgctcgaa
     7381 cagtttggat caaggggctg tttccttgaa agactgcttg gagaacagtt aattcatcgg
     7441 aaaattcagc tgcttgagaa agataaccaa tacggtaatc agttggataa aacactgttt
     7501 gccgatcacc gtcgccgcta tcgataccgg caataatatt taagagactt gttttacccg
     7561 tcccgttcgt cccaatcaac ccgatacggt ctttttcgtg gaccaaaaaa gaaatctggt
     7621 caaacaaatc tttttcacca taagtttttt tgaggtcagt tacttttaat tctttcaaca
     7681 tgttccctcc ttatcttgga tattgtatca taaaaatagt gaacttcagg aaaatttttt
     7741 tggtatttgt tctttttgtg ttctcaaaaa aagaacagga ccgattcgat cctgttctga
     7801 tatgttttgt tttttataaa acttgaaaat ttttaccttt ttattttatt ggactcgttt
     7861 ttttgacttt gctcgaaacg caaatcggaa agaaaagtta tcagatggtt tgttcatcgt
     7921 ataagattct aacaaattga tattcctgcg atagctgttc gcagaaaacg tcgtgatttt
     7981 ctcagcaact tcgtattcat caatgatttt tctttcaaga taataacctc ttagcatttt
     8041 ttttgtaaac agactatctt gctggatcat ccacgaagaa atcaaattcc ccgagttcat
     8101 ttttattcct tcaagtttgc gttgctggat aaaaaattga accatcgttt catcataaat
     8161 atcagtcaag ttatctaaac tttcaataat caactgagta tttttttgat atgtctctct
     8221 gatttcttgg atatcttcat cactaataaa tgtctgacga tcagcatgtc ttctttgcca
     8281 aaacattttc ggatggagaa cgattcgcat taacctacgg acaacaatga accaaaagcc
     8341 gataaaagac aaaatctggc ttgtgataga atgttcgaat cgagaaagaa aacgggagta
     8401 aaaacgataa ccattagaag aaagcttgcc cgaacgataa ctttcatcca atccatcccg
     8461 ttcgatcgac aaaatcagtg cttgtatttc ttgtacttct tgcttttcat catcggttaa
     8521 atcttccaag tacagatcac gtatccgttc ttgataattt tctatcactg cttcagtggc
     8581 taaaaattct tttgtattac gatcaatttc ctttatttct tttcgcaaaa cttcaacgac
     8641 ctcttctaaa atcatcaaag cattcaaatc cgtgcttttc gcttcctctg tttccgtcag
     8701 cataggcaag acaatgatcc ctatgaccaa agtcactaag atgacacccg ccgtcagaaa
     8761 caaaagcaaa gaccgctggt aaaacatcat attattgatt gaaaatggca aaataaaaat
     8821 cgtagccaaa ctgactgttc cctttacacc gccaaatgtt aagatcaaga tctcgttcaa
     8881 ctgttttttg aactttttac tgccatcttt gaatacataa aaaagactga tagaaatgaa
     8941 tctgatcaca aaaagcatga cagagattaa tacgataatc gctaataaaa gaccgtttgg
     9001 atataaaccg tctccccata ctggcgagaa cacttgcgtc aattcgattc ctaaaaatat
     9061 aaatactaat gcattcagtg taaaagcaat agttgtccat gtactgtggg acaagctcga
     9121 caattctgca tcaaacacag agattttcct aaaaccagaa gcttggagaa tacctgctgc
     9181 cactgcagca atgattccag atacatcaaa tgcctctgcc aatacatagg ctagaaaagg
     9241 aagcaaaagt tcgatcagca gataccctgt gacatcttgt gctgatgctt tttcgatcaa
     9301 atgaattatt ttttgtttga accagacaag caaaaagccg atcaaagctc ccccaatact
     9361 agaaactact agactcatcc ccgcgtttac tgctgaaaaa gatccggtga tcagcgcacc
     9421 aagagcaaac tggaaggcag taacaccaga tgcatcattc aaaagacctt ctccttccaa
     9481 aatatgcatc gcctttttag ggatattcac tcgtccagaa agagaactga ctgctaccgc
     9541 atctgtcgga cctaacgctg ctccaaaagc aaagcaggca gcaagtggga cacttggcaa
     9601 caggaatgat aacgtcgcac ctaccccgac aagggtcaaa atgactcctc caaatgccaa
     9661 gaacaaaatt gtcccgaaat ttttgagaat cgaagaaata tctgcttttt ctccttctct
     9721 aaataacaat ggtgcaataa tcataactaa gaacaattca ggttcaaaat cgattgattg
     9781 tccccactct gtcagaccta ataccactcc aaggaaaatt tggatcagtg gagctggaat
     9841 cattggcaaa atgcggctag ctatgttcga taaggtgatc gttactgcaa atacaataac
     9901 taactcagca aattccatcg tccatcccct ttatttttta ttttgtgtaa gcatttttaa
     9961 aaattgttct tttttcttta aaattttggt ttcgactact tcaacctgtg tttggttacc
    10021 ctctaattca cactgtacac gttttgactc caaacgaagc agatcatgtt tcagacgttc
    10081 caactgacga tacatcaata tatttctttt acgcaactta caaagtattt gacggcggtc
    10141 atccatcgaa agggcgccat agcgatcatg caacagtttt ttttgttgtt caaaactctc
    10201 tctcattttc cagatctcct atacacaaac ttccatcatt atagcaaagt tctttttgga
    10261 tgaaaaatat ttcattgttt catcgtttat tgctagttag aaatttccat aaatttttca
    10321 acgcctcatt ctattccaaa tagtatcaga tatcaagtaa aacttaagaa agataaaagt
    10381 ttctgacaaa agaatgttta cttttccatt cctgcctttc ttgttctttt atcacaacaa
    10441 aaaaatgggg ctgcgacaaa aatcttgtca cagtccctta ttctgaataa acggtgtgga
    10501 agagacagcc tatcagtagt aataagacac atctctttca cagcttcatt tttgcgtaaa
    10561 aatcgattta ttcgaaaaat tccccgtata catcttctac acgttgatct ggattgatca
    10621 cgatatacaa gttacctgtt ttttcgctga tttttgttga ttgatattga ttgtctaatc
    10681 cttctgttcc tttatctaga tatgggaagt acacaagatc aggtgtgcca tttcggtaaa
    10741 ggatttccat tcgctcgata tcttcgtatt tcaatgcgcg attaaacatg cccatttcca
    10801 gtccgcctag gttgatatca tgtgtcgcaa ctgcgtcccc ttcatggaag atttcgattt
    10861 taaatccagc acacggatgg atttcaacaa attcactgcc attgattcgt ccaaaacttg
    10921 ttgtgatttg tttgatccaa agatcgccga tatgacggcg gttgatcgtc caggtttctc
    10981 catttctaaa ataaaatttt agcgctgtca tttctttact cattaaaaag actcctttat
    11041 gatgtaattt attcttgtac tacctcagtt tagcacaaat ttatgtaagt ggtcacatct
    11101 tatgtttttt cttctaatga cgtatctgcc cactttaagt agccaacaaa gctgagcaga
    11161 tacagtctag tgttttttaa atcaattttt ctttcgataa aaaatccaaa acctgttttt
    11221 tatcattttt tattctttgt tctacgacgg ctttttcagc taattgcaaa gcttcactaa
    11281 cccacttacc aggttttttt ccagtttctt ctagcaagat tttaccatct actgctaatt
    11341 cgcttaatga cttgataggt aaacgatcat aggattctcc tacggcatcc atctgaggtg
    11401 tttcgtcaaa atagatcatc aattcttcta cttgcagtgc atgttttttg cctaagaagt
    11461 acaattcgaa tggttccaaa gctccagttt ctcgttgtcg tagtccttgg acaagtgctt
    11521 gaacgtcttt aatcatttgg ttagaacact tccacgattt caaaaaggaa cgaatctcat
    11581 tttcttttag acccagttgg ctgatcaata atgtccatac ttggctctct tctttcaatg
    11641 ggacggatgg taattcagca aaacgtaaaa gtgcttcgcc ttcttctttc aaactgggac
    11701 agcaaatgta gcactctgtt tcaataaatg catggattcc cgcggcccgt tcatttccca
    11761 agagcatctt gatgaattcc acatttatac gttctacaga tattttacct aatagctcgt
    11821 ggttttcttt gatggcgtag aaggtttctt cctctaattt gaaccctagt tgactgacaa
    11881 aacgcaaccc gcgcatcatt cgtaaggcat cttcatggaa ccgctcatga ggattgccta
    11941 cagctcgcaa gatctgttct tctaaatctt gcagaccatc gaacaaatcg atgatttctc
    12001 catcttcttt caaagcaaac gcattgatcg taaaatctct tcttttcaaa tcttctgcca
    12061 acgaacgaac gaattcgaca tgatctggtc tgcggtagtc ctgataagtt gattccgtcc
    12121 gaaatgtggt gatctcatat tgttcatcat tttctaatac caacacagtt ccatgttcga
    12181 tcccaatatc tatcgttttt ggaaaaagct gtttgatttc ttctggaaat gcgcttgttg
    12241 cgatatccac atcatggatt ggctggttca gcaagatatc acgtacactt ccacctacaa
    12301 aataagcttc atatcctgca ttctctaatt ttttcagaac tggaattgcc ttttggtatt
    12361 ccagaggtaa ttgttttaat ttcatttttc caacctttcc tgcgtgtttc ttgtactgag
    12421 ataaagactc agttgcatgg tgactgtttg acaagtccgt ttcagagtct tcaaggccag
    12481 gaccacttca tcttgtttac tcgtccttac gctcaaagcg attatggtct ctttgattga
    12541 atttttccat gtttttttca aaaacttcag ttaagtcgat attcaatgaa ttagccatga
    12601 tcatcgtaac gaataaaaca tcaccaagtt cttctgctac cgagttaact ggttcagaag
    12661 cctttttgct tttttctcca taagtgtggt tgacttctct tgccaattca cctacttctt
    12721 cagttaatcg agccatctga gctaatggag agaaataccc caccttgaat tgctggatat
    12781 attcatccac ttcctgctgc atactgtata aagaacgctt tttatccatc tttttccctc
    12841 caaaactatc ttaccaaaac tttaacgcaa atcctagata agagcagttg agaaaatcga
    12901 atttccatgc tacatttaga aaggaatttc taaatggaaa ttctgtgaaa atacataatt
    12961 gaaagctgga attcatatgg tcaaacgaaa aacagtaaaa gaagttcttt ttatcattat
    13021 aggaacaagt atttatgcat ttggtcttgt ttacttgaac atcgctaatc atttagcaga
    13081 agggggcgtc tcagggatca ccttgatctt aagagcttta ttcggaattg atccagctta
    13141 tacgacttta ttaatcaaca tcccattgat tttactagga ggaaagatcc tcggtaaacg
    13201 ttccttggcc tatacagttc taggtacact ttctttgtca ttctttttat ggatctggca
    13261 aagggttcca ctagaaatca atttacagca tgatctactg atcgtttctc tacttgcggg
    13321 tttagttgca ggtgtaggaa gcgggatcgt ctaccgcatg ggtggaacaa caggtggcag
    13381 cgatattatt gctcggattt tagaacaaaa ttacggcatc actatggggc gttccctatt
    13441 ggcatttgat atcatcgtct tacttgcttc attaacctat atcgacttga aacgtatgat
    13501 gtatacgttg attgtttcgt atgttttcag tcgtgtgatt gattcgatct tagatggcgg
    13561 atattctgca aaaggtctac ttgtcgtctc caacaaaagc gaagagattg caccactact
    13621 gatgaccggt ctgcaaagag gtgtgacttt tctttctgga gaaggcgcat tctctggtaa
    13681 ttcaaaaaaa atcatctata tggtcgttag ctctagtgaa ttaactgaag ctaagaggat
    13741 cgtccacgag attgacgaaa aagctttcct ttcaatcatc aatgtgcatg aagtggaagg
    13801 gcaaggcttt acttatttga agccacagac aagattactg aaaaaactat aagaaaaaag
    13861 acaaagatag gctatgagga aagattc
//
LOCUS       DAKBVG010000063        13812 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000063 DAKBVG010000000
VERSION     DAKBVG010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.063"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(63..1268)
                     /locus_tag="OUD09_001280"
     CDS             complement(63..1268)
                     /locus_tag="OUD09_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HCT9692558.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
     gene            complement(1291..1444)
                     /locus_tag="OUD09_001281"
                     /pseudo
     CDS             complement(1291..1444)
                     /locus_tag="OUD09_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(1447..2832)
                     /locus_tag="OUD09_001282"
     CDS             complement(1447..2832)
                     /locus_tag="OUD09_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCT9692559.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            complement(2861..3247)
                     /locus_tag="OUD09_001283"
     CDS             complement(2861..3247)
                     /locus_tag="OUD09_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCT9692560.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            complement(3263..3577)
                     /locus_tag="OUD09_001284"
     CDS             complement(3263..3577)
                     /locus_tag="OUD09_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCT9692561.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            complement(3930..4409)
                     /gene="rlmH"
                     /locus_tag="OUD09_001285"
     CDS             complement(3930..4409)
                     /gene="rlmH"
                     /locus_tag="OUD09_001285"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCT9692562.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYGLAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            4837..6126
                     /locus_tag="OUD09_001286"
     CDS             4837..6126
                     /locus_tag="OUD09_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HCT9692563.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVMMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(6165..6770)
                     /locus_tag="OUD09_001287"
     CDS             complement(6165..6770)
                     /locus_tag="OUD09_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HCT9692564.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVASYGMICSARELHLPNAPEKKGILVLPEDAVVGEVFPK"
     gene            complement(6966..7430)
                     /locus_tag="OUD09_001288"
     CDS             complement(6966..7430)
                     /locus_tag="OUD09_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCT9692565.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKALEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKQMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYAIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(7452..7772)
                     /locus_tag="OUD09_001289"
     CDS             complement(7452..7772)
                     /locus_tag="OUD09_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCT9692566.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VIKG"
     gene            complement(7915..8994)
                     /gene="pepA"
                     /locus_tag="OUD09_001290"
     CDS             complement(7915..8994)
                     /gene="pepA"
                     /locus_tag="OUD09_001290"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HCT9692567.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            9194..9544
                     /locus_tag="OUD09_001291"
     CDS             9194..9544
                     /locus_tag="OUD09_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HCT9692568.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKTSFLKEGAIEGAWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLDISRKKGAID"
     gene            9729..10682
                     /locus_tag="OUD09_001292"
     CDS             9729..10682
                     /locus_tag="OUD09_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCT9692569.1"
                     /translation="MESREKIAVLIPCYNEEATISTVIEDFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAAHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVKNTITYLYKTKIRDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMCKDYKPLLFFGIWTIFFFLFGLVMGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYQNKEMK"
     gene            10695..12482
                     /locus_tag="OUD09_001293"
     CDS             10695..12482
                     /locus_tag="OUD09_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9692570.1"
                     /translation="MHKKKENLFPTFFATLFASGFAFFTSYGLGTLNPTVIIGLAMVL
                     LLAFKQYGIELFSIKNKVAAIARLFFSAAFALTLSIGRQIQFQYVSANYTKTTIADGS
                     IFYFLVYFSIFVYSFLFLQSIYLSMPKLETFFTAQNTQAFSSRKYFLFFTVFLVLCWL
                     PYLLALYPGVVLPDSLSSVAQSMGDVPISNHHPVLFTLLVKLFVHDLPTDTINHGVFL
                     FSIFQTLITASAISYSLCWLLKRNIHFFPVLISLCYFAFAPVFPIYALNMQKDTLFSI
                     ACFLFSLAVFDFIFQREKPTILNQIYVIILALLTTYLRNNGIYVVTGTGLICGLIILK
                     QKRYQAFFQVVLGYTVIAGLLIHPLMARYAIPTETAEQLGVPLQQISRTIVMDGDLSK
                     KDKAFMNQLLPIKDYEAYSPSLADSIKWHKNFNNEFLNTHKKEFLSLWARNLPSNIKI
                     YTDAYILETFGFWVPGTKNSYGFLDTRVNTNNYGMKQVDLIEHLTGLSGMKKIIDARD
                     FFGSGTLLWLAIGSALLAIFKKKYRYLLLFLPGMLVFATIMIATPVAFSLRYVFVLAL
                     GLPVYILSPFLLDKREKMKTDIGDTHETI"
     gene            12469..13671
                     /locus_tag="OUD09_001294"
     CDS             12469..13671
                     /locus_tag="OUD09_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9692571.1"
                     /translation="MKLFKKIFFAFVLLIASYCVLLTITFFIPQHSIESNAEKSLQMV
                     EKEGMYPSINQGNMLGTRLDNFTDHLMIRKTKADPELNSLENAMSMADYPRYWHGYQL
                     FLRPLLLGMSLGSIRMIYAAILFLLIGLTSYFLTKCSDIYLTTALLISLAIGNAATFF
                     FSMQFSNIWILTLLSVLLFLTKPQLSNTNQQLFLYFFVIGSLANFFDLLTTPVISWGI
                     PVIIIYYVTNKYLMDKRSSLSKQVGSFVFTGIFWGLGYGLTWVAKWILSSIILNKNIV
                     KDAINQILFRTEGNDKYPLHRLEMLKSNIRLMYPKVAILLLLITCLVFLYFAYRKRNS
                     WRPFQLLVLFVLAAVTPYIWYNILANHSQIHYWFTYRTQIITSFAILSAFAFLIPPEK
                     SKDELNYF"
BASE COUNT         3961 a         2700 c         2532 g         4619 t
ORIGIN      
        1 aggttttgtc caaaattcat aaaaatgttc tcctctctat attcaatgaa tttgtatttg
       61 agttattttt ttgttgttat cacgtcctgt tcttttactg actgttgctt caaaatctgc
      121 ttgtgtcggt ctgtcagttt cgcatggtcg agaatgtctt ttacaacctg cgtctggttg
      181 atttcatcaa gtttaatcgc aacctttaag gtcggggcaa cttgatgaga tagccagttc
      241 agcgtccttt ggaaggagta aggctctggt tttgtggtta gttttaatcg ttcacgattg
      301 ttcccaataa accaagccca ttcttcattc agtttccaat cagaacgagg tttggaatcg
      361 tctttatcta caaaacggat ataccgattg ataattttaa aggcggtatg ctctggattg
      421 tcatagacga gtaaatcacg gactgcataa taggcacgct catttttcaa tcgaatctca
      481 aaacggtttt ttacttctgc gtcttcaatg ggaatatcat ttttcttgta ctgctcgtag
      541 tccttttcat agatacagaa ataaacttca ctttgtaatg aaccgatata gagggtgttt
      601 cccatacatt ccttttcctc tttgcgtacc agttcgccac tgcgatagct tttaaaactg
      661 cggaagacgg agatacattc ttcctgttgg cacttttcag tgagtacagg gatatttaaa
      721 atccctgtct tatcgttaat ggcaaggtca aggcgtttca tcacaccgcc agccaccaaa
      781 acgtccataa agaactcata ccagcttctt tgttgtgcca gaagatagct ttcaaattgt
      841 ctgcacccac gacctttcaa ttccaccaga actcctttgt ccagttcatg ggagcaaagg
      901 acgaatatgt cgcctaaagc ataatgctct gaataagaat agaaaccata gtcctcatga
      961 agaaaatagg acagtttcag ttgtaagatg ttttcgacca cctgctgtac gtctgttgtc
     1021 ggaaagcgaa ttcttacata atcaaacagc atttcaaggg gagcgtcggg attgaagcgt
     1081 tccagagctt cccaaaggga ctgctgtaaa tcctctgatg gcttgacttt tcctgtttca
     1141 atatcgctta gatactgcct tgtaatacca gtcgcaacag ctaaacggtt ttgagatagt
     1201 ccataagcca agcgtttttc ttttaaatgc tgtaaccaag tttgttcatt cagtaaaaat
     1261 ccctccaatc aaaaaggcgt atgtcaactt ttaaagccca tttgacatac gctgaaattt
     1321 tgtaaatccc ttgtaaccaa aggattttct aatgtttttt tgactgtttc ctgtcgattt
     1381 gtacccccct gttagatacc gaggggttaa gtgctggcgt ggctattgcc acaccagcca
     1441 gcaagatcag tccacacctg cgacttccgc ttcgcacgtc gcctgcgtgg actgtctgct
     1501 gttggataac tttttaattt cctccaagaa atcatatcct tttggtacaa ggggagtata
     1561 aaactctgat atgacacttg ttcctacatc aacatagcca cgacctttga ttcgctttaa
     1621 gaagaaatcc ttttgtacgt cactgccaaa catcatgcca tagcccattt cagacatacg
     1681 acctaaagcc actctgaaat taaactgatc acggattccg tcgcctaaat attttgcgtc
     1741 tggacgttga caagccagta ttagaaagaa gccagcttga cgacctaaca tgacaatctg
     1801 tttcagctta ttcataactg cggtgttttc ttttgttccc agcatttcca tgaaagcgac
     1861 gtattcatca aagattaaga agtgtgccgg gagacctaag taagcataat ttttgccagt
     1921 cttatagttc ttcatctgct tcatttcctc actacgtttc atcatttctt catagaatgt
     1981 ttcaatgcaa gaaagcaagt cttcttttct atagtagaca tttgccatca cagaacctaa
     2041 gtccgcaagg tcagcatttt tcgggtcaag aatatacagt tttgaatctg tatgaagcaa
     2101 ggcttcaatc agtgtcagta taaagtaagt tttaccgcca cctgtaccac cagcaatcaa
     2161 catatgaggg agcttatcat attcccacca tacgtttttc attaagcgaa gtttaccatc
     2221 tttagcttct acttcatcaa tagaaatacg actggctatg gtgtcataga gcaaagtata
     2281 ttccacatag gaatccttta actctttatc cgtcagctca cagtacaagc cactctctaa
     2341 tttcttttcc aagtgtaaga gttggtcttg atattttccc agcgtgattt ccacccgtat
     2401 ctgtatcaag ccatttttaa gtcgataata cattttaggg aagtaggtta tcttttcctt
     2461 tgtacgacca gcactatctt taaagaaacc ctctgttttg acctgttcag attcatacca
     2521 cttgttttca agtatcatct ttgccagttt ttgacggtgg taaagttgtt taaccgtatc
     2581 atagcgaacc cgtttgaata caaacgctac cagcaagcag ataagaattg cgacactgaa
     2641 actgataatt aaatagggaa tgtcaatctt atctgcttgt gataggttaa aatcctgcca
     2701 gttgatctgc tggattgtct tcacatgaaa cagtccgaca accagcagga aaacaggcag
     2761 gagtgacgct atcgtaaaat gaaagactaa atctttacca gatgggcgaa tccttttacc
     2821 acgctgtttc atgcgaaaaa gtctcctttc tacctagcga ctatttgtct tgtgtcggtt
     2881 ctttctttgc ttgtggttga gctttgaatg aactagaatc ctttgtcagc acaatatcgt
     2941 ctgccttgat ataccagtca acatctgctc cttgataggt ggcagtagca acggtgtccg
     3001 caatgggatt gataagttcc acccgtgcgt tataatcaaa ctctttcaaa ggcacgctgg
     3061 caggaatact tacttgaatc atgcgtcctt gtcctttgga ttttaagtca taggtacgtt
     3121 ccttgatttc atctgaaacc gacccgtctt cattttggat tctcacttca cgacgtagag
     3181 cagagaattt caattctcca aaagtcgtgt ctttatctaa tacaatgcca tttgctaatc
     3241 tcatcatttt tcctctcttt ctttattctt ttatcatgtc gtcagcatgt aaaaggtaat
     3301 ttgtaaaacc acgagtgccg attttgtagc cctctgcggt aatacgtgga ttgactaact
     3361 tcacacgttc ctcaaagccg aaatgttttt cgccagcttc agcaggaagc accaccacaa
     3421 tatcatctgc tctttgaaca tcagaataga gattatagct tcttgataag acagttagcc
     3481 gtccgttgat tcttcgctga acgactttat cctcgccagc aaattctaaa ttgccgaatg
     3541 ttttttccat gttgggaatc acaaatttaa gttccatatt tttacctatc ctttcttttt
     3601 tattggctga atgaatgttt gatggtctta aagagtgggg aacgaccttt tgattcttga
     3661 ttttttgttt tcataagttc acttcctttc aaaatcgggt aaaaaaatag acacctcatt
     3721 ttttgaagtg tctacctatt aaatattcaa attttattgg aagtatcttt atatcttcac
     3781 ttttcaagga taaatcgtcg tatcaaagct cattcataag tagtaaatta gtagtaaatt
     3841 gagtggtttt gaccttgata aagtgtgata agtccagttt ttatgcggat aactagattt
     3901 ttatgctatt tttctcttaa aaaatatcat tatttgtggt atggagcccc cgcgttgatc
     3961 cggaaggcac ggtagatttg ctcgacgagg atcaggcgca tcaattgatg tggatacgtc
     4021 attttgccaa aagagatctg agcattgctt cttttcatga cggcttcgct gagtcctaaa
     4081 gaaccgccga taacaaaaac aaggtggctt tttccttgga tacctaattg gtcgatttgt
     4141 ttagcaaaag attcgcttgt gggatttttc ccttcaattg ctaagccata gacgtattct
     4201 tgttctttga ttttcgctaa gatccgttct ccttcttttt cttttacttg tctcatttgc
     4261 gcttcgctta aattctccgg ggctttttcg tcaggaactt cgatcaattc gattttcgca
     4321 taggcattca gccgtttgac atactcattg ataccttgga tcaagtattt ttcttttaat
     4381 tttcctactg aaataatttt gatattcatg ttttctctcc tttgatcttc acgctcactt
     4441 tgtttattat aatcaatttt ttcgtctata tgtttatttt gactattttt gttaaaaaga
     4501 tttccccaac agttatgcac aacatccaca ggtttatcca cagaaaacag cgttgtttta
     4561 ccttatttca tcacattcat taggcaaaca tacgttttat aagaatttat ccactttata
     4621 cacaggctgt ggatatgttt tatcccttat ttttccaatc ctatccgcgt agttttacac
     4681 aatatctgtg gaaaaagaaa atcaaatatt ttcatctgaa aacaatgatt tttcctaaag
     4741 tttttcataa aaagttcaaa gtctcaaagt agactaaagt caacaaataa gactatacta
     4801 acagtagata aatgaatgta attggaggag gaacacatgg atagaaaaaa tgtgacacca
     4861 aagatgaaaa aaaataaaaa taatagcctc tggcgtaagt tgggtctggg gcttgttgga
     4921 gggatcgttg gaggactact gacagctggc attttttatg cagcgatggg cactggcaac
     4981 aatagttcta ctgctacaag cggcaatcaa aattctgccg gtgaaacagt tgtggaaaat
     5041 gtcaaagtca atgtagattc tgatatcacc aaagcggtag ataaagttca agacgctgtc
     5101 gtttctgtta tcaacttaca aagtcaaaac caatcaagcg gttttggcgg attattcggg
     5161 cagcaagaag aaagtagcag ctcagatgat agtaatttgg aagcttatag tgaaggaagc
     5221 ggcgtaatct acaaaaaaga cgggaatact gcttatgtag tcacaaacaa tcacgtagta
     5281 gatggacagc aaggtttgga agtcatgatg aaagacggta cgaaggtaaa agctgaatta
     5341 gttggaacag atgcctattc agatttagcg gtgttgaaga tcaattcaga caaagtcgaa
     5401 acggttgcct cttttgggga ctcaagtgct ttgaaggttg gtgaacctgc gattgcgatt
     5461 ggttccccat taggatctga atatgcgaat tctgtcactt caggaatcat ttcttccttg
     5521 aaccgacaag taacaagtac gaatgaatcg aacgaaacag tcaatatcaa tgcgatccaa
     5581 acagatgcag cgatcaaccc gggaaattct ggtggtcctt tagtgaatat cgaaggacaa
     5641 gttatcggta tcaactcaag taagatcgca agtacttctg aatcgacttc taatgtaagc
     5701 gtagaaggaa tgggatttgc catcccaagt aacgatgtag tgaatatcat caaccaactg
     5761 gaaaaagacg gaaaagtgac acgtccagct ctaggtatca caatggtcga cttatctgcc
     5821 gtttctactc agcaacaaga acaaatcttg aagattcctt catctgtaac taacggtgtc
     5881 atcgtgacaa atgtccaaac agctacaccg gcagaaaaag ctgggttgaa acaatacgac
     5941 gtcattacta agatcgatga caaagaagtt tcttctggtg tagaacttca atctgctttg
     6001 tataagaaaa aagtgggaga ttctgtcaaa atcacttact atgaaggctc gaaagaaaaa
     6061 acaactactg ttgaattgac gatcgatcaa tcggcattaa aacaaaatca aaatagtgaa
     6121 aattaataaa aaagaggttg tgacaaagac ttgtcacaac ctctttattt tggaaatact
     6181 tctccgacta cggcatcctc aggcaatact aagattcctt ttttttctgg agcatttggc
     6241 aaatgcaatt cacgagcaga acaaatcatt ccatagctgg ctacccctcg caaagttcct
     6301 ggccagatca tcattccatc tggcatcatg gctcctggtt ttgcaacgac aactttttgt
     6361 ccagcttcga tatttggtgc accgcaaaca atttgcaata ctgtcccatt gtccacttct
     6421 gtctcagtaa tcgataaatg gtcagagtca ggatgcggga cgcatgtttt tacatacccg
     6481 acaacgatct tcggttcatg atcagcttca agctgttctt cgaatcctgc ttctttaatc
     6541 aatcgattca atttcgctaa ttcttcatct gttaagctta cttggccgtt cccttcaatt
     6601 gttagatgat cggagatatg gaaaaagttc caagcaacaa cttgaccttg ttcatctttg
     6661 atccgagcaa cattccattt acgttcgaca gtgttttctg ttcctttatc gtcggctatg
     6721 ataaccatca aagtgtctcc gacgtgtgct ttgttataag caaaaatcat gttctttcct
     6781 ccattatcct ttgctgagat ttatcttaac aaaaaattgc attcactgca tgagattttt
     6841 ttcatgcttt atttccgaag aagcttcttc tggaacacca tttattcgta acaagggact
     6901 gtgacaagac ttttgtcaca gtccctttac tgtttgatta aaaaggttat acaagttcca
     6961 cttgcttaat tactcggctg ttcttcttcg tcagaaacga tcaagacatc acaaggtgaa
     7021 tgacgtatga cgtagctggc tacacttcct gtcataaagc gttcgactgc atttagtcca
     7081 gactgcccaa ccatgattag atcgattgca tatttttttg gcaatttgac tgccatggat
     7141 tcacgaacag agccgtatgc aataattcct tctatatttg tgtaatcaac agattttcca
     7201 tattctttac attcttcgat catttgcttg gctgtttcag tttgctgatc gactagattt
     7261 tgatcaatag gagcatagcc cataaaatta taaaattgct gatcgattac gtttgctaca
     7321 tatactttcc cattattacg gcgagccact tctaacgctt ttttgaaagc agcaagtgct
     7381 tgttcactac cgtcaatacc tactaaaatg ttcttatatt cttgattcat cttgacccct
     7441 cctaacagat gtcagccttt tattacactt tctaggaacg attcgatttc ctgttttgtt
     7501 ttacgatctt tattcactag acgacctaat tcttttccgt cttggatcac gacaaaactt
     7561 gggataccaa aaatgttcca ttcagcggct acatctatgt acttgtcacg gtctacttca
     7621 ataaattgcc aatcagaaaa gtcattttcg atttcaggca tttgcggttt gataaaacgg
     7681 cagtctccgc accagccggc agtaaagaaa aatacacttt ttccagtttc tacgtagcct
     7741 gctaattctt ctaaactatt tgggataatc attctttttc ctctactttc tgtttatgta
     7801 tttttcaatt aaattataca cctttctttc gaactaacaa acattgatcc tctttttata
     7861 aaaaaaatct gacaaaaggt tgcttttgcc agattttata gtttcactat cgcgttattt
     7921 cccgtatacg atcgtgttca ctgttgattt atctaaacct ttcaatgttt gaatcaacat
     7981 ttctcgtgca gcttcaaaat cgcgaatact gaacatcgtt tgatgcgtat ggatataacg
     8041 tcccactacg ccaatcactg tacttgggat tccttcattt tgtgtatgag cagcacctgc
     8101 atctgtaccg ccttttgaaa caaagtattg ataagggatg ttgtttgttt cagcgatatc
     8161 tagcaagtac tcacgcaaac gaggaagcat gatcaaacct ggatcttgga tacgcatcaa
     8221 tgtaccttct cctaaatgtc cgaatgtgcc atttttcgtc actgtgtcat ctgctgcaga
     8281 gcagtctact gcaaagaaca gatctggctt gaatttattg acagaagctt tagaaccacg
     8341 caagcctact tcttcttgga cattcgcgcc tgcaatcaat gtatgaccta atgtttcatt
     8401 ttgtaacgct tctaaagctt ctaataccat tgtacagcca tagcggttgt cccacgattt
     8461 gctgatgata ttcttcccgt tggctgtttt gatcgtttca gcgtaaggaa cgattgtgtc
     8521 tcctggaaga acaccgtaac tcattgcctc ttctcttgat tcaaacccag catcgaacaa
     8581 aatatctgtt acttctactg atttttgtcc gcttgttcct cgtaataaat gtgggggaac
     8641 ggaagaagaa atacatggat aattcttacc agtagaggtt ttaagtgtaa aacgttgagc
     8701 agaaacaacg taagggttcc atccgcctag aggtacgact ttaaaaagcc cgttttcttg
     8761 gatttgtgtc agcatgaatc cgacttcatc catatgtgct gcgaccatta cacgaggtgc
     8821 atccgcttct tcatggtggc gcagcccaaa aatgccgccc aaaccatcta actgtaactc
     8881 atctaccagc ggttccatat gactttccat ataggcgcga acgtcttgtt caaaaccgct
     8941 tgttccttgt aattctgtta actctttgat tcgttgaaat gttttttctt ccatatgcta
     9001 ttcgtctaaa tttcagacta aactcctctc agacttatat tgatctgcgg ataaaatatg
     9061 attttttctg cagaccgatc gtatttatta tagcttattt tttctggtag cggttattta
     9121 tttcaaagaa aatatgagag tgtggtaaaa taaacacaaa gagtagcaag tgagaagaag
     9181 gaggtatatc cacatgtatg aagaaagcga attaagctat tttaaaggcg ggttagccgt
     9241 tgggatcggt ctaggattat tgagtggggt agcttcaacg atttggtatc atcgcaaaaa
     9301 agtgttatca gcagaccaag tgcttgaaaa tatcaaaaca tcttttttaa aagaaggcgc
     9361 aatcgaaggt gcttggatct cttttgaaaa agaacctatt cggaaattcg ccgttcggtc
     9421 gagtgcttat cggggtgggc tttcccggat agaagatggc gaaactgttt attatgaatt
     9481 tttagcagat gctcatacag gaaccgtatt agacatttca agaaaaaagg gcgcaataga
     9541 ttaatacgtc aaaaaaatga aataacgaat acgcacccac tatctaaaag agaggtatcc
     9601 tatccttctt ttatagtggg tgatttttat aaaaataggg ctatttcctc ttggaatagc
     9661 tagtatttgt agttattata cggtattctt gaaataagat tagttttatt atataaggag
     9721 agtgacaaat ggaatcacgt gaaaaaattg ccgtgctcat tccttgttat aacgaagagg
     9781 cgacaatttc aactgttatt gaagatttta aaagggaatt accagaagca gatatttatg
     9841 tctatgataa taattcgacc gatcgaacat acgaattggc cgttcaaggc ggagcaatcg
     9901 taaagaaaga accacgtcaa ggaaaaggga atgtaatccg tcaaatgttt tttgatattg
     9961 atgccgatta ttatttgatg gtagatggtg atgatactta tcctgcagaa gctgcacatg
    10021 gtttgcttga gaaattacgt tctggcgaag cagatatggt cattggcgat cgtctatcaa
    10081 atggaaccta ttttgatgaa aataagcgtc ctttccatga ttttgggaat aatctggtta
    10141 aaaatacaat tacttactta tataaaacaa aaattcgcga tgtcatgacc ggctatcgag
    10201 gattcaatcg aatttttgtc aaaagcttcc ctattatgag ttctggtttt caaatcgaaa
    10261 cagaattgac gattcatgct ttagataaaa aattcaagtt cgttgagatt ccaatcgatt
    10321 atcgcgatcg tcctgaaggt agcgaatcaa aattgaatac attctctgac ggattcaaag
    10381 tgatcatgat gatcatcaaa atgtgcaaag attataaacc tttgcttttc tttgggatct
    10441 ggaccatctt cttcttttta tttggattgg tcatggggat tcctgtgatt cgtgaatata
    10501 tgctgacaag ctttattaca cgcatccctt ctgcgatttt aagtactgga ttgatgactt
    10561 tagcccttct ttcattagta acagggttga ttttggatac ggtcgtcaca aatgccaaga
    10621 aagaatatga attgaattta taccatgtct accacgaata tcaaaataaa gaaatgaagt
    10681 aggaggcctt ttttatgcat aagaaaaagg agaatctatt tcctactttt tttgcaactt
    10741 tatttgcgtc aggttttgct ttttttactt cttatggtct aggaactttg aatccaacag
    10801 taatcattgg tttagccatg gttttgctac ttgcttttaa gcaatatgga atagagttgt
    10861 tttcaataaa aaacaaagtt gcagctattg cccgattgtt tttttctgca gcttttgctc
    10921 ttacactttc tattggcaga cagatacaat ttcagtatgt ctcagcaaac tatacaaaga
    10981 caacgattgc tgatggaagt attttttact ttcttgttta tttctccatt tttgtgtatt
    11041 cttttttatt tttacaatcg atttatttat caatgccaaa actggaaaca ttttttactg
    11101 ctcaaaacac acaggctttc tcttcaagaa aatatttttt attctttact gtctttttag
    11161 ttctgtgttg gcttccttat ctgttggcat tgtatcctgg agtggttttg cctgattcgt
    11221 taagttcggt cgcccaatct atgggtgatg ttcctataag caatcatcat ccagtcttat
    11281 tcaccttact ggtcaagcta tttgttcatg atttaccaac tgatacgatc aatcacggtg
    11341 tatttttatt ttcgattttt caaacattga taacagcttc agcgataagt tattctcttt
    11401 gctggctgtt aaaaagaaac atccattttt ttccggtact cattagttta tgctattttg
    11461 catttgcacc ggtttttcca atctacgcct tgaacatgca aaaagatact ttgttctcta
    11521 tcgcttgctt tttattttcc ttagcggttt ttgactttat ttttcaacga gaaaagccaa
    11581 cgattctcaa tcagatttac gtaatcatcc ttgctttact gaccacctat ctaagaaata
    11641 atgggattta tgtagtaacc ggcacaggtt tgatttgcgg cttgattatt ttgaaacaaa
    11701 aaaggtatca agcattcttt caagtggtgc tgggttatac agtgattgct ggactactca
    11761 tccacccgtt gatggctcgt tatgctattc ctacagaaac tgctgaacaa ttaggtgttc
    11821 ctttgcagca aatttctaga actattgtta tggatggtga cttgtctaag aaagataaag
    11881 cattcatgaa tcagctactc ccgataaaag attatgaagc gtattctcct agtcttgcag
    11941 actcaatcaa gtggcataaa aattttaaca atgagttttt gaatacacat aaaaaagaat
    12001 ttctatcact atgggcaaga aatttaccaa gtaatataaa aatctataca gatgcttata
    12061 ttttagaaac gttcgggttc tgggtacctg gtacaaaaaa tagttacggc tttttagata
    12121 ctcgtgtaaa cacgaataat tatggtatga agcaagtaga tttgattgaa catcttactg
    12181 gattgtcagg aatgaaaaaa atcatcgatg ctcgtgattt ctttggttcc ggcacattac
    12241 tgtggttagc tatcggcagt gcacttttag caatctttaa gaaaaaatat cggtatttac
    12301 tcctttttct tcccggtatg cttgtttttg ctactattat gattgctact cctgtggctt
    12361 ttagtttacg gtatgtgttt gttttagcat taggtctacc tgtttatatc ctctctcctt
    12421 ttttactaga taaaagagaa aaaatgaaaa cagatatagg agatactcat gaaactattt
    12481 aaaaagatat tttttgcttt tgttctcttg atcgctagct attgcgtact actgacaatt
    12541 acttttttca ttccgcagca ctctattgaa tcgaatgcag aaaaatctct acagatggtt
    12601 gaaaaagaag gaatgtatcc ttctatcaat caaggaaata tgctaggcac tcggttagat
    12661 aattttactg atcatttgat gatacgaaaa acaaaagcag atcctgaatt gaactccttg
    12721 gagaacgcga tgtctatggc ggattatccc cgttattggc atggttatca gcttttttta
    12781 cgacctttac tgctagggat gagtttgggg agtattcgaa tgatttacgc agccattctt
    12841 tttctgctga ttggattgac gagctacttt ttaaccaagt gtagcgacat ctatttaacg
    12901 acagccttgt tgatttcgct tgctatagga aatgctgcaa cctttttctt cagtatgcaa
    12961 ttttcaaata tttggatttt gactttattg tctgtattac tatttttaac aaaaccacaa
    13021 ctctccaaca ccaaccaaca attattcttg tatttctttg tcattggttc tttagctaac
    13081 ttttttgact tgttgacaac accggtcatc tcttggggga tacctgtgat cattatttat
    13141 tatgtaacaa ataagtattt aatggataaa cgcagttcat tgagcaaaca agtaggcagt
    13201 tttgttttta ccggtatctt ttggggatta ggctatggtt tgacatgggt ggctaaatgg
    13261 atcctatcat cgatcatatt gaacaaaaac attgtaaaag atgcaatcaa ccaaatcctc
    13321 tttcgaacgg aaggaaacga caaatacccg ttgcatcgat tagagatgct gaaaagcaat
    13381 attagattga tgtatccaaa agtagcaata ttattgctat tgattacttg tcttgttttt
    13441 ctctattttg cttatcgtaa acgaaatagc tggcgtccgt tccaacttct agttttgttt
    13501 gttttagctg ctgtaacgcc gtatatttgg tacaatattt tagctaatca ctcacagatt
    13561 cattattggt ttacgtatcg tacgcaaatc atcacaagtt ttgctatact tagtgctttc
    13621 gcctttttga tccctcctga gaaatcaaaa gacgaactaa attactttta agaataccga
    13681 tgtactaatt catttttaaa cataaaaact ccagctgtca gagatagccg gagttttttg
    13741 gctctataat ttatgggact gatgaatcag aattgattct tcagccccat tttcatttta
    13801 ggtattaaaa aa
//
LOCUS       DAKBVG010000064        13626 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000064 DAKBVG010000000
VERSION     DAKBVG010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13626
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.064"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            332..946
                     /gene="gmk"
                     /locus_tag="OUD09_001295"
     CDS             332..946
                     /gene="gmk"
                     /locus_tag="OUD09_001295"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCT9692572.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYINKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            951..1256
                     /locus_tag="OUD09_001296"
     CDS             951..1256
                     /locus_tag="OUD09_001296"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCT9692573.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            1509..3917
                     /gene="priA"
                     /locus_tag="OUD09_001297"
     CDS             1509..3917
                     /gene="priA"
                     /locus_tag="OUD09_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCT9692574.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGDNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            3932..4423
                     /gene="def"
                     /locus_tag="OUD09_001298"
     CDS             3932..4423
                     /gene="def"
                     /locus_tag="OUD09_001298"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCT9692575.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            4416..5354
                     /locus_tag="OUD09_001299"
     CDS             4416..5354
                     /locus_tag="OUD09_001299"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCT9692576.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            5354..6712
                     /gene="rsmB"
                     /locus_tag="OUD09_001300"
     CDS             5354..6712
                     /gene="rsmB"
                     /locus_tag="OUD09_001300"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCT9692577.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            6725..7465
                     /locus_tag="OUD09_001301"
     CDS             6725..7465
                     /locus_tag="OUD09_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HCT9692578.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            7462..9531
                     /gene="pknB"
                     /locus_tag="OUD09_001302"
     CDS             7462..9531
                     /gene="pknB"
                     /locus_tag="OUD09_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HCT9692579.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            9696..10595
                     /gene="rsgA"
                     /locus_tag="OUD09_001303"
     CDS             9696..10595
                     /gene="rsgA"
                     /locus_tag="OUD09_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HCT9692580.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            10608..11258
                     /locus_tag="OUD09_001304"
     CDS             10608..11258
                     /locus_tag="OUD09_001304"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCT9692581.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            11259..11897
                     /locus_tag="OUD09_001305"
     CDS             11259..11897
                     /locus_tag="OUD09_001305"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HCT9692582.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            12052..12906
                     /gene="mreC"
                     /locus_tag="OUD09_001306"
     CDS             12052..12906
                     /gene="mreC"
                     /locus_tag="OUD09_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCT9692583.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            12910..13419
                     /gene="mreD"
                     /locus_tag="OUD09_001307"
     CDS             12910..13419
                     /gene="mreD"
                     /locus_tag="OUD09_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCT9692584.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
BASE COUNT         4501 a         2506 c         2995 g         3624 t
ORIGIN      
        1 tattaagcca aatttatgca aaaatgtgag aaactatttt cacaaaattt ttcttcatac
       61 tcgaaactaa tcggcttctt cacgacctct tatacacggc gttcaaaagc ttgcatctgc
      121 gataataacc ccaaccaaac gaaaaacatg agaagctgtt tatgtttggc tgttcttatt
      181 acttgatgca ggacagcttt tttacgacct cttggtacag ggtagctttt ttaccaattt
      241 ctagctataa ttaaagataa ccttgaaagt tgcagaaaaa aagtgcaaaa taaacgataa
      301 gtacaaaaag actagttgaa aggacgtcgt catgtcagag cgtggattat taatcgtgtt
      361 gtctggtcca tccggtgtag gaaaaggcac agtacgaaaa gcaatttttg agagtgagga
      421 taatgatttt cagtattcga tctcaatgac tacccgtaaa atgcgtgaag gggaggtcga
      481 aggcgttgat tatttcttcc gaagcaaaga agagtttgaa tcaatgatcg aagctggaga
      541 gatgctggaa tacgcagaat atgtcgggaa ttattatgga actcctttgt catatatcaa
      601 caagacattg gatgaaggaa aagatgtttt cttggagatt gaagtccaag gagcaaaaca
      661 agtcaaagaa aaagtgcccg atggtgtatt catctttttg actccgccag atttagcgga
      721 attaaaatca cggatcgtag gccgaggaac agatgcagat gaagtaatcg aagaacgaat
      781 gaaagttgca aaagaagaaa tcgaaatgat ggctctttac gactatgctg ttgtaaatga
      841 tgaagtgccg ctagcagttg aacgaatcaa aaacattatt gccagtgaac attttcgagt
      901 agatcgcgta atcggtaaat atattaaaat gttaaaggag atgtagtctt atgatgttaa
      961 aaccttcaat tgactcacta ttagatcgtg taaattcaaa atattctcta gtgattttag
     1021 caagtaaacg cgcgcatgaa ttggatgcaa gtgcacagcc tactctagat tcttttgatt
     1081 cagtaaaaag cgtcggtcaa gctttagaag aaatcgatgc aggaaatgta gtcaatgatc
     1141 cacatcctga attaaaacgt gaacgtttga agatggaaga agaggaacgt caagcacaaa
     1201 aagagcgtga acaacacgaa ttggaatcac gtatccgtga agagcaaaaa atgtaataaa
     1261 gaaaaataga gcgaacatct aaaaagaaaa gcaaacccag ctgtttcctt ttcaaacatt
     1321 cgccacttat tgtctggtag aaaaggctgt gtctgaactt tttgacacag ttcttttttt
     1381 tcttatctgc tgttaatttt ttgttaggat ttggcagaag ataagacaaa tccatcgatc
     1441 tctagtaaaa taggcaagga actttttgtc gcttgataag aaaagacaaa aaaaggagga
     1501 aacgaaccat gacattaaca gctgaagtca ttgtggacgt acctactatg cagaccgatc
     1561 agccattcac ttatttggtt ccctctgaaa tggaaacagc tttgcaagtc gggatgcgag
     1621 tagaagtccc ttttggtaat ggcaatcgtc atgtccaagg atttgttatg gctatccaaa
     1681 aatcagaaga atcagctaat ccatctttga aggcagttat tcgtttgcta gaccttgctc
     1741 cggtggtcaa tgaagaatta ctttcattag ctgattatat gaaaaaaatt acttacgcat
     1801 tcaaaatcac ttgtctacaa acgatgcttc cgagtgtgat gaaagcagaa tatgataaat
     1861 tgatctatcc tttggcagac acaccagaag ttcaggtatt gtttgatcag cgagaagtga
     1921 tttcctggaa ggaagcagaa gaagaaggca gcctttctca attgattcgc tggcgccaag
     1981 agcaattagt agatatcaaa tatgaagtcc atacacgaaa taaagtaaaa acgattcgtt
     2041 tggtccgttc tttgttaact gaaaagcaaa tcgaagaaga atgggcaaaa ttacggcaaa
     2101 atgccaagaa acaaaaagaa cttcttttgt gtttatctga actgagccaa gaagaaccaa
     2161 ttgcttattt caaaaataaa ggaatcagta ctgctgtctt gaatcaagga aaagaaaagg
     2221 gctggctgga gtttatagaa tcagaacgtt atcgcgaccc ttataaagac cgagtatttg
     2281 accaaacgac ggcattggaa ctgaatgcag aacaaaaaaa tgctgtcgaa caaatcatca
     2341 cagcaggtca gcagcaaaaa gatcaggtct ttttgctgga gggaatcact ggcagcggga
     2401 aaacagaagt atatcttcag gcaatcgctg atgttttgtc agaaaataaa acggctatta
     2461 tgttagtgcc tgaaatcgca ttgactccgc aaatggtcga gcgattcaaa ggacgcttcg
     2521 gagaatcagt tgcggtctta catagcggac tttcccaagg tgaaaaatat gatgaatggc
     2581 gcaagattga gcgggaagaa gcgcaagtag tagtaggcgc acgatctgct atttttgccc
     2641 cgctgaaaaa tatcgggctg atcattatcg atgaagaaca tgaatcaagt tacaaacaag
     2701 atgagacacc gcgttatcat gcgagagatc tagcaatttg gcgcagtaaa tatcatcatt
     2761 gcccaatagt tctaggaagc gcgacgccct ctttagaatc aagagcacga gctcaaaaag
     2821 gcgtttatca gttattgcag ttaaaccacc gagccaaagc agctgcacag cttccagcta
     2881 ttgagatcgt agatatgcgt gaagagtttc agaatcatcg gacctctact ttttctgcta
     2941 atttgcagga aaaaatacaa aatcgattag acaaaaaaga acaaactgtc ctactgctca
     3001 atcgtcgagg atattcgtct tttgtgatgt gtagggactg tggatttgtt cttccatgtc
     3061 ctaattgcga tatctctctt acactgcata tggatacacg ttcaatgcgc tgccattatt
     3121 gcggacatga agaacctatt cccaatcgct gtcctaattg tggcgacaat aaaatccgct
     3181 actatggaac aggtactcaa aaagtagaag aggaactccg agaactttat ccgcaagcta
     3241 ggattttgcg tatggatgta gataccactc gaagaaaagg ggcacacgaa cagattttac
     3301 aaaaattcgg tgcaaaagaa gcagatatat tattagggac acaaatgatt gctaaaggtt
     3361 tggactttcc agaagtgaca ttagttgggg tgttgaatgc tgatacctcc ctgaatttac
     3421 cggattttcg ttctagtgag catacattcc aattgcttac ccaagtttca ggacgagcag
     3481 ggcgagcgga aaaagcaggc gaagtcgtga tccaaacatt caatcctcaa cattacgcca
     3541 tagaattagc taaaaaacaa aattatgagc agttttatca gcaggaaatg catgtacggc
     3601 atcgtggagg ctatcctcct tattacttta ctgtgaaaat aaccgccagc catcctgaag
     3661 aacaagtagc ggcaaagaaa atctttcaga ttgccaatca gctaaaagaa gtgttaagcc
     3721 ctcaaagctt gctattaggg ccaacgccca gcatgatttt gagagtgaaa aaccgatatt
     3781 attatcaact aatcatcaaa tataaacatg aaccgaatct tcctcatgtt ttggatgaaa
     3841 ttttgaatgg aagccagaaa gaacagcgac aaggtctttt tgtggcaatt gacaacgaac
     3901 cattaaactt tatttaagaa agggccaaat tatgcgctat cctatattga tccaccctaa
     3961 tgataaatta aaaagaactg ctcagccaat cgatgtaatc acggacgaga caatcgcttt
     4021 attagacaat ttatatgaga caatgattgc gaatgacgga atagggatcg ctgctcctca
     4081 agtaggacaa aacaagagaa ttgctgtgat cgaagtagat gaaggcgaga aatttgaact
     4141 gattaaccca gaaatcatcg aagcaaaagg tgaaagcctt gacgtagaag gttgtctcag
     4201 tattccgcat gtctatggaa cggtcaagcg agcagatgaa gtgaccgtgc gttattatga
     4261 tcgagacgga gaagagatag aagtgacggc ttttggctat ttagcccgtg ccttccagca
     4321 tgaaatcgat cacttagacg gtatcttgtt cattgagaaa atgatacagc agatcccgga
     4381 agaagaactt gaagaatata tggaggaaca tcttgatgac taagctagta tttatgggca
     4441 cacctgcttt ttctgtgccg attcttgaag ggctgataga atcaggttac gagatccagg
     4501 cagtggtaac acagcccgac cgtccagttg ggcggaaacg agtaattacg ccgacaccgg
     4561 taaaagaagc ggctctgaag cacggtatcc gtgtgctgca gccagagaaa atctccggtt
     4621 cgccagaaat ggaagaaatc attgaattgg tgcctgatgt gatcgtcact gcggcatttg
     4681 gccaattctt gcctgaaaag ttattgcaag taccaaaatt aggggcaatc aatgtccatg
     4741 cttctttact tcctaaatat cggggaggtg cgcctgttca ttatgccatc atgaatgggg
     4801 agaaagaaac tggtgtaacg atcatggaaa tgatcaaaaa aatggatgca ggcggaatct
     4861 atgcgcaaga aagcatacca atcactaaac aagatgatgt agggacgatg tttgaaaagc
     4921 ttagcctttt aggaagaaag ctattgctag aaacattgcc caatattttg gatggacaaa
     4981 aaccagtacc tcaagacgag tcagaagcaa ctttttcgcc aaacatcaca cgggaacaag
     5041 aagcaatcga ttggcataaa actgcagaag aaattgacaa ccaagtacgc ggaatgcgtc
     5101 cttggccaat tgcattcacg acctatgagg gcatacgctg gaaattatta tcggttgaag
     5161 tattggcaga aactacagac aaagcacctg gtacgatcat ccggaaagac aaaaaaaatc
     5221 tatggattgc ttgcggtaaa cagaccgtct tagccatcaa tgaattacag ccagctggta
     5281 aaggaaaaca ggccgtacat gatttcctta acggaagcgg tcagcaagtt cagattggtc
     5341 agcaggtgaa ataatgaaaa aaatccctaa aaaaatgaaa aaatcagtac gatatatggc
     5401 attactgaca ttagagcgag tgaacaaggg tggcgcctac tcgaatcttt tgttgaatga
     5461 gatgatcaaa caaagcgagc taaatcaaaa agatgtgggt ttatttacag aattgatcta
     5521 tggtacgatt agccatcagc gactattgga attttacgtt gcaccattga tcgctaaagc
     5581 aaaaaaagtg gatgactggg tcaagacgct tctttatctt tctgtatacc aattagaatt
     5641 tttagataaa gtccctgcac atgctatttt aaacgaggcg gtcgaaatcg caaaagtaaa
     5701 aggaaatcct gggacaggga aatttgtaaa tggtgtcttg aggaattatc agcgtcaagg
     5761 agcaccagat ctgcaaggca ttgctgatcc tatcgaaaga ctttctgtca aaatcagcct
     5821 gcctctatgg atgacgaaac gactggtgga tcaaatcgga tacgaagaaa ctgaaaagct
     5881 cggtttatcg ctatatcagc ctagccatgc tagtgcccga gtagatacac gccgactttc
     5941 cagagatcag gccatcactg ttttgcaaga agaagagatc acagctgaaa aaagccaagt
     6001 cagtccatac ggtatagtag cgaaaaaagg gcatttagct tctagctcat tgttccacga
     6061 tggcgtcatg acgatccaag atgaaagttc gatgcttgta gcgccgtcta tgcaaatcga
     6121 gcctgaacat catgtcttag atgcctgtgc agcacccgga ggtaagacga cgcatattgc
     6181 ttctttttta gatccgtcat gcggtggaga agtggtcgct ttagatgtac acgaacacaa
     6241 agtcaaactc attgaagata atgcagcacg tctaggtgtc tcttctgctg tctacgctca
     6301 aaagatggat gctagagaag tcagtaaaca gttcgaagca gagcagtttg atcggatttt
     6361 agtagacgcg ccttgttcag gtcttgggct gcttcggcga aaaccagata ttcgttataa
     6421 aaaacagccg gatgaattag cgaatttacc aaaaatccaa ttggaaattt tagaaagctg
     6481 cgcaccaact ttaaagtctt ccggtattct gacttatagc acttgtacga tcttaaaaga
     6541 agaaaaccaa gacgttgttt ctgctttctt ggaaagacac cctgattttg agaggattga
     6601 tgttttgacc aatcctatgc tacaatcatc aatcgaagag aaaatgttga ctttgtatcc
     6661 gcatcagttt tacacagatg gatttttcat ttgttgtttg cgcaaaaagt aatgaggtga
     6721 aaaaatgcag attgaatatc aatcagatgt aggaagacga cgtaacacaa atcaagacta
     6781 tgccagtgta tttaccagcc gttcaggtct caagcttgct gtattagcag acgggatggg
     6841 tggtcatcgt gccggagaca ttgccagtca gatggcggta acgaatctag gcgaagcttg
     6901 ggaaaaacaa gaagctgttg acgatgaaaa aatcgcccaa tggttcattc aatcgattca
     6961 agaagaaaat atgctgattt atcagcgtgg acaagaacag ccggaatata acggtatggg
     7021 aacaacaatc gtagcggccg ctctgtcaga tgactggttc acgattgcca atgttgggga
     7081 tagcagagct tatttgattc gtgaagataa aatcatgcaa ttgacggagg atcattcttt
     7141 agtcaatgaa ctagtcaaat caggagaaat cagcgaagaa atggcagtga accatccaag
     7201 gaaaaatatt ttaacacgtt ctgtcggcat gcctggaaca gtcgaagtag atgtttctac
     7261 tcatctttgg caactaaaag atcgactgct gttatgttca gatggcttga cgaacatgct
     7321 gtctgaagaa gaaataggaa cgatcatcaa tcaagaaggc ccattgtctc ataaagtaac
     7381 tgagctgatt gatcgtgcca atgaagcagg cggagcggat aatatcaccg ttcttcttat
     7441 cgaatataag gaggacagtg tatgatcgag ttgggcaaaa aattaaatgg ccgttatcaa
     7501 atcactggaa atatcggtag cggtggtatg gcaaatgtat ttttggcgca cgatttaatc
     7561 ttggatcgtg atgtagcagt taaagtctta cgatttgact ttcaaaatga ccagactgcg
     7621 attcgacgat ttcagagaga agctttagca gcaacagaac tcgttcatcc aaacattgtt
     7681 agtgtttacg atgttggtga agaagacaac atgcagtatc tggtgatgga atacgtcaaa
     7741 ggaatggact tgaaacgata tatccaaaca cattatcctg ttccttatga aacagcagta
     7801 aatattatgc agcagatcct ttctgctatt tctttagctc ataaccatca aatcatccac
     7861 cgagatttga aaccccaaaa cgtactgatc gataatgaag gtgttgtaaa aatcacggat
     7921 ttcgggattg ctatcgcttt atctgaaaca tcgatcacgc agacgaatac gatgctggga
     7981 tctgttcatt atctgtcccc tgagcaggcc agaggaagta tggcaacgaa gcagtcagat
     8041 atttatgctt tagggatcat cctttatgag atgttgacgg gtagcgtacc gtttgatggt
     8101 gaatctgctg taacaattgc tttaaaacat tttcaagatg atcttccatc tataaaagca
     8161 cttgatccaa atgtgccaca agcactggaa aacgtgattt tacgggcaac ggctaaagaa
     8221 cctgcagacc gttataagtc agccgaggaa atgtctgatg atctttctac agtattatca
     8281 ccagctcgtg caaacgaaga aaaatggcag cctcatgtga tggataatga aacaaaagtt
     8341 atcacacccc ttacggaaga tgcaccaatg cctgattcat tcaaatctat gcctctgcca
     8401 aaagacaagc cagaaaacga aaccttgcca gaggaggaag aagttctgcc tgatcctaaa
     8461 aagaaaggac agaaaaaatg gtggctgatc ctattagcta ttcttgctgt tttaggaatc
     8521 ggaacagctg tattctttgc ttctggcggg cgaggagaag ttgttgttcc tgatgtttct
     8581 gatctttctg aagcttctgc tagagaaaca ctcgcaaaag cgaaattaga agtagcaaca
     8641 aaaacagaag aaattgcaga tgacaaaatc gaagaaggta atgtagtgaa gacagacccg
     8701 gcagcgggaa cgacagtcaa gcaaaaacgc gaagtcactc tgtatattag tagcggcacg
     8761 aaaaaaatca aattagatga ttacagtggt aaaagttatg aagatgccga agcaagtttg
     8821 aaagaattag ggttttctgc tgacttgatt caaaaagaag aggaattcag ttcagaggta
     8881 gaaagcggaa tgatcatttc gcaatctcca tcagaagata cagaagtcga tcctaaaaca
     8941 gacacgatta ctttcacagt cagcaaagga ccagaaacag tgactttgag tagtcatgta
     9001 ggagaaaact acgaaactgt tcgtaattgg cttgttacac aaggattcaa tatcaataat
     9061 attagtgcta cttatgacta tagcgacact gtaccatcag gttcgatcat cagtcaggat
     9121 ccagcctcgg gtgaagttgt agcagaagaa acttacatca attttgtcgt aagccaagga
     9181 acagagccta aattgaatga tatctccggc tatacaaaaa gcgaagcaca aagttattta
     9241 gctagtgtcg gtgcagaata tatcgggcat gagacttccg aatattcaaa cacggtggat
     9301 aaagataaag tgattcgtac agtaccaggg gcaggaacga caattaccaa aggaatggtt
     9361 gtgaacgtaa tttactctaa aggaccagat ccagcgtctt ctagttctga aaaagactct
     9421 tcagacagtt ctagcagctc aagtagctcc agtagcacaa gcaactcttc aaaagaagaa
     9481 tcttccagct ccacaagttc atcagctagc acctcagaaa gttcaaaata agtgctgaat
     9541 aaaatatatc agaagggaat gcgacattga tttgttgcat tctctttttt gtgtttctat
     9601 ctcttttgat caattttcaa gccatatact ttgagaagtt tgcatggaat aacacctact
     9661 ttcctcttcg tatggtaaaa taaacaaaaa cggaggtggc acttatcaaa ggacaaatca
     9721 gaaaagcaat cagcggtttt tactatgtat acgcaaacgg agagacctat caaacaagag
     9781 gaagaggaaa tttccggaat cggaaaatca cgccgcttgt cggagatcag gtgatctttg
     9841 agagtgaaaa tcaaacagac ggctatcttc tggaaatctt gccgagaaaa aatcaattag
     9901 ttcgcccacc agtcacaaat gttgatcttg gaatcattgt cacaagtatc gtagagcctg
     9961 cattttctta taatctactt gaccgctttc tagttacctt ggaatacgag tcgatcgaac
    10021 cgattatttt tttgacaaag accgacttgt cagacgaccc agaaaaaatc gaagagatca
    10081 agcaaaccta tactaaaata ggctatccgg tgattgtacc caatcatacg ggtgatacga
    10141 cagaactgat ccgttacttc ccagaacggc tatcggtctt tatgggtcag tctggagcag
    10201 gaaaatcgac gctactgaat caaatatcac cggaactgaa tttagcaaca ggagagattt
    10261 ctgatgcact tggaagagga cggcatacaa cacgtcatgt ggaactgatt cctatctatg
    10321 gcgggttagt agcagataca ccaggattta gctcgattga ctttttgaca attgaagcag
    10381 gagaactgcc gagacaattt cctgaatttg tagaagcttc tgtccattgc aaatttagag
    10441 agtgcatgca tgctaaagaa cctggctgcg aagtaaaaca tagagtagaa acaggtggta
    10501 tcgcacagac acgttacgac aattatttgc aattcttaca agaaatcgaa aaaagaaaac
    10561 caatgtatgc aaaaaaagaa ggtaaacgaa aataggagga aacaagaatg aagatagcac
    10621 catccatttt gagtgcagat tttgccaatt tacaaagaga cattgagtta gtggaaaaag
    10681 gaggcgcaga ttacatccac gtagatgtaa tggatggcca atttgtccca aatatcacat
    10741 tagggccaaa tattgttcag gcaatccgtc ctgttactaa attaccgtta gacgtccatc
    10801 tgatgatcgt tgatccagaa aactacattc cagcatttgc aaaagctgga gcagatatca
    10861 ttactgtcca cgtcgaagca actcctcata ttcatcgggc acttcaaatg acaaaaagct
    10921 taggagttaa agctggagta gttatcaatc caggaacacc agtctccatg atcaaacatg
    10981 ttttgccttt ggctgatcaa gtactagtca tgactgtcaa tcctgggttc ggcggacaaa
    11041 gctttattga agaaacagta gaaaaaatta cggaactcgc tgatctacgt agagagaaca
    11101 attggactta tgcaatcgaa gtagatggcg gaatcgttcc agaaacagca aaaatctgta
    11161 aagatgcagg ggcggatgtc ttcgttgcag gatcatatat ctatggagcc gaaaatccag
    11221 tcggacaaat cgaacgatta aaagaggcat tagactaaat gaaaatttta ttagctgctg
    11281 gaggtaatcc gcaaaactgg ccgcagtctc tgctgtccaa atacgatgcc tatgtcggga
    11341 tagaccgtgg tgcgctgtac ctgcttcaag caggctaccc agtagatctg gcagtgggcg
    11401 attttgattc attgtcgaac gaagagcaga aggaagtatt tgcaaaagcc aaagagtata
    11461 cccgttcccc tgcagaaaaa gacgatacag atacacagtt aggtatctta aaaacatttg
    11521 aacgttaccc gcaagcggaa gtcactttga ttggagctac gggcggaaga ttggatcatc
    11581 ttttagccaa tctctggtta ggtatggaac ctcgcttcaa accctttgcc catcagctta
    11641 ttatcagaga ttgtcaaaat tatttgtctt attatttgcc tggagagcat atagtaagac
    11701 agataccaga tatgaagtat ttagcttatt gctgcttgac tcccgtctcg aatcttacat
    11761 taaaaggcag caagtatgta ttggaaaata aagaagtaga atatccgaca tcttatgcaa
    11821 gtaacgaatt tctttcagat accgcagaat tttctttttc agatggtatg attgcagtta
    11881 ttcaaagcaa agattgatcc ataaacaaat aaaaaaattt gtctttcagg ctaaaaatga
    11941 cagaatctat tatttgctaa aaaaaagctt aagaggagca acttaccttt tgtttcacgg
    12001 catttagtga taaaattgaa cacaagagtc taattaattc gaggtgtctt tgtgaagaag
    12061 ttcaatccta ataaaaatat tattattacc ttggttattg ttatcatcgt ggtgacgata
    12121 ctaagtgtga ccgctgcgca gcgagcgaat gaaggaaagc ccaatttctt ccaatccatc
    12181 gttaatgaca gtgttgcaat ggttgataaa acaatctctg ccccagtacg gtgggtacga
    12241 aatggagtag attctgtcca taatcttttt tcaacttatg ccgaaaatga gcggctaaaa
    12301 gaaaaaatcg atagctatga tgacgtttca cagcaaaaca agaattacga gagagaaatc
    12361 gaagcgttga aaaaagaact tgagttgaat cagacgctga ctagttacga aaaagtaacg
    12421 gcaaatgtca ttactcgttc gcctgacagt tggcaggaca tgctgatcgt agacaagggt
    12481 tctaaagacg gtatcgaagc aaatatggca gtcatgtcgc aaaaaggatt gattggccga
    12541 gtgatcgagg tcaatacggc ttcgtctaaa atcgaattac tgtcatcctc taatgaaagc
    12601 tccaatcatt ttccagtacg ggtatcttcg gctaatggcg aagcgtttgg tttgcttaaa
    12661 aactatgatg aaaagctcca tgccttagtg gtgacccaat taactggtga tacggatatc
    12721 aaagaagggg atgttgtcca gacatccggt cttggaggga attctccagc taacttgccg
    12781 atcggtacgg ttattaaaac gaaaccagat agttatgggc tggatcggga agtttatgtg
    12841 aaaccttatg cagaaatgta tgacgtgtca gttgtgacga ttgtccaacg gctagcggag
    12901 gattagtcca tgttgaagaa agaaacaatg aagtactatc tgccaatcgt tttgttcttt
    12961 ttgatgttga tagatggtca tttaacaaga atgctagggg agtggtcgaa aggcacctat
    13021 atgtcaaatg cccactttct gatattggca ttattatgtt gcagtatggc gtttgaaaaa
    13081 cgttatttac tgattaccac gattgttctc ggggctatct atgacgctta ctatattggc
    13141 gttatcggta tctatgcagt agctctccct ttaattgtat ggttgatgta tgtaatgaaa
    13201 gacgttatcc atgtcaacat ctttactgaa tttttcagta tgatcatctt tgtcacgggt
    13261 tatgaattgt ttacgatggt ggtccagttg atttttaaat tagcagtagt aaataacacg
    13321 tattttatta caaggttttt aggacctaca ctgctgttga acatgattat atttgtatta
    13381 ttcatttttc cctttaagaa attattcagt gatgaataat ttcttttttt atttttaggt
    13441 cgaaagcgaa aaagaatcgt tgttatctta agaaatgcta gacttaacgc ggctttttca
    13501 tgatcatttt ctgaaacgaa cagttttgtc atttttaaaa gttgttttga aacatttttg
    13561 taacataact attatatcgt tgacacactg ctatggtaca atttttatat cgttaagaaa
    13621 gactta
//
LOCUS       DAKBVG010000065        13587 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000065 DAKBVG010000000
VERSION     DAKBVG010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.065"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            14..256
                     /gene="yaaA"
                     /locus_tag="OUD09_001308"
     CDS             14..256
                     /gene="yaaA"
                     /locus_tag="OUD09_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HCT9692585.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            243..1367
                     /gene="recF"
                     /locus_tag="OUD09_001309"
     CDS             243..1367
                     /gene="recF"
                     /locus_tag="OUD09_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCT9692586.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            1364..3310
                     /gene="gyrB"
                     /locus_tag="OUD09_001310"
     CDS             1364..3310
                     /gene="gyrB"
                     /locus_tag="OUD09_001310"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCT9692587.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            3330..5801
                     /gene="gyrA"
                     /locus_tag="OUD09_001311"
     CDS             3330..5801
                     /gene="gyrA"
                     /locus_tag="OUD09_001311"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HCT9692588.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            6037..6336
                     /locus_tag="OUD09_001312"
     CDS             6037..6336
                     /locus_tag="OUD09_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCT9692589.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            6386..6898
                     /gene="ssb"
                     /locus_tag="OUD09_001313"
     CDS             6386..6898
                     /gene="ssb"
                     /locus_tag="OUD09_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCT9692590.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            6924..7160
                     /locus_tag="OUD09_001314"
     CDS             6924..7160
                     /locus_tag="OUD09_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCT9692591.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            7303..9276
                     /locus_tag="OUD09_001315"
     CDS             7303..9276
                     /locus_tag="OUD09_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HCT9692592.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            9282..9734
                     /gene="rplI"
                     /locus_tag="OUD09_001316"
     CDS             9282..9734
                     /gene="rplI"
                     /locus_tag="OUD09_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCT9692593.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            10008..11375
                     /gene="dnaB"
                     /locus_tag="OUD09_001317"
     CDS             10008..11375
                     /gene="dnaB"
                     /locus_tag="OUD09_001317"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCT9692594.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            12155..13447
                     /locus_tag="OUD09_001318"
     CDS             12155..13447
                     /locus_tag="OUD09_001318"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCT9692595.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT         4521 a         2465 c         2993 g         3608 t
ORIGIN      
        1 tgagggatgg gcattgaaac aacaagtcat tctaaatacg gaattcatga cgttgggcca
       61 aatgttgaaa gaagtcacag ttatcggtag tggcggacaa gcaaaatggt atttggcaga
      121 aaacactgtg ttagtcgatg gcgaaccaga aaatcgtcgt ggacgcaaac tttatcccgg
      181 catgatggtt gaagttccag aagtaggaac tttttttatg gtaaaaaaag gaaacgacga
      241 taatgaggct gaataagctg tatttaaaaa attatcgaaa ttacgaagag ctgaacatcg
      301 aattttccaa gaacctcgtc atctttttag gagaaaatgc gcaaggaaaa acgaatcttt
      361 tggaaagtat ctacgtatta gctatgacgc gaagtcatcg gacaagtagt gagcaggaat
      421 tgattggctg gactgacgat caggcgatga ttcaaggaga gatcacaaaa ggttcatcca
      481 ctcttccatt agaaatctta ttatctaaaa aaggccgaaa aaccaagatc aaccatatcg
      541 aacagaaaaa gttaagtact tacgtgggac aaatgaatgt cattttattt gcaccagaag
      601 atctttcttt agtaaaaggc agcccgcaga ccaggcggaa gtttttagat atggaattag
      661 gccagatcaa tccaatctat ctttatgatc ttgtccaata ccaagcaatt ttgaaacaaa
      721 gaaaccagta cttgaagcaa ttgaatgaaa aaaaacaaac tgatttgctt tatttggata
      781 ttttgtccga acaactagca gcgtttggca gcaaagtatt gaaagcaaga gcacagttcg
      841 tcaagcgatt ggagttttgg gctaattctt tgcatcagca aatcacccat caaaaagagc
      901 agctggaaat cgagtatctc accgcagtcg attctttaga aacacatacg caagagcaaa
      961 tccaagaaca atttctcgca cttttgaacc aaaataaaaa gaaagacctt tttcggggaa
     1021 caacgacagt tggtcctcat cgtgacgatc tttccttttt tattaatcag aaaaatgtgc
     1081 agacttatgg atcgcaagga cagcaaagaa caactgcact aagtgtcaaa ttagcagaaa
     1141 tcgatttgat taaagaagag acaggggaat atccaatcct tttgttagat gatgtgatga
     1201 gcgagctgga tgataatcgt caactccatc tgctagaaac aatcgaagga aaagttcaaa
     1261 cgttcttaac aacaacgaca cttgaccatg taaaaaataa aatgactgtt gaacctgaga
     1321 ttttttatgt tcaacaaggg aaactaaaag gagaagaaac tgaatgacag aagaaagaag
     1381 tttagtagaa cgcgctaaag agtatgatgc cagtcagatt caagtacttg aaggacttga
     1441 agccgttcgt aaacgacctg gcatgtatat cggttcgact agttctgagg gtctgcatca
     1501 ccttgtttgg gaaattgtcg ataattcgat cgatgaagta ttagcaggtt ttgcaacgaa
     1561 aatccatgtg attattgaaa aagacaacag tatcaccgtt attgacgacg gtcgggggat
     1621 acctgtagat atccaggcaa aaactggacg tcctgcagtt gaaacggtct ttactgtttt
     1681 acatgctgga ggaaaattcg gcggtggtgg gtacaaagtt tctggtggac ttcacggagt
     1741 aggatcttct gttgttaacg cgctttctac acagcttgat gtcaaagtat acaaagaagg
     1801 aaatgtgtac tatcaagaat atcgtcgtgg agctgtagtg gacgacttaa agattatcga
     1861 acaaacggat cgccatggaa caacggttca ctttacgcca gaccctgaaa tttttacaga
     1921 gacaactgag tttgattttt caaaattggc tacccggatc cgtgaacttg ctttcttgaa
     1981 ccgcgggatg cgtatatcga ttgaagacaa acgagaagaa gaaccagtca tcaatgaata
     2041 ccattatgac ggcgggatta aaagttatgt cgaatacttg aatgcaaaca aaaccgtgat
     2101 ttttccagaa cctgtatatt tggaaggtga acagcaagat atcgcagttg aagtctctat
     2161 gcaatataca gacggctacc attccaatat catgagtttt gccaacaata tccatacgta
     2221 tgaaggcgga acacatgaat caggattcaa gactgcttta actcgtgtga taaatgatta
     2281 tgcccgaaaa caaaagctga tgaaagaaaa tgacgacaac ttgaccggtg aagacgtgcg
     2341 agaaggattg acagctgtta tttctatcaa gcatcctgac cctcaattcg aaggacaaac
     2401 aaaaacaaaa ttgggcaatt ccgaagtacg gacagtcact gatcggttat ttagcgaaca
     2461 tttcatgaaa ttcttgctgg aaaacccaag tgtgggccgt caaattgttg aaaaaggatt
     2521 actagcttcc agagctcgtc tagcagcgaa acgtgcgcgt gaagtaacga gacgtaaagg
     2581 tgctttagag attagtaatc tgccaggaaa attagcggac tgctctagca acgatcctga
     2641 aaaatgcgaa ttgttcatcg tcgaaggaga ttcggccggc ggatcagcta agcaaggtcg
     2701 taatcgagag tttcaagcca tcttgcctat tcgtgggaaa atcttgaatg ttgaaaaagc
     2761 aagcatggac aagatcttgg caaatgaaga gatccggtct ttatttactg ctatgggtac
     2821 agggtttgga gaagattttg atgtatcgaa agctcgctac cacaaattag ttatcatgac
     2881 tgatgccgat gtcgacggcg cgcatatccg tacattgctg ctgactcttt tttatcgtta
     2941 catgcgtcct attgttgaag ctggatacgt ctatattgct cagccacctt tatacggggt
     3001 aaaacaaggg aagaatatta cgtatgtaca accaggaaaa aatgcagaag aagagttgaa
     3061 acaagtcgtt gcttcacttc ctgcctcacc aaaaccaagc gttcagcgtt ataaaggact
     3121 tggagaaatg gatgatcatc aactatggga aacaactatg gacccaagca atcggatgat
     3181 ggcccgcgtc agtgtggacg atgctattgc agcagatcag atatttgaaa tgttgatggg
     3241 ggatcgagta gaaccccgtc gtgcattcat cgaggaaaat gcacactatg tgaagaactt
     3301 agatatttaa tcatgaagga ggaaaaagaa tgagtgaaga aatcagagaa aacatccatg
     3361 atgtcaatct aaccagcgaa atgaaagact ccttcatcga ttatgcgatg agtgttattg
     3421 tcgcacgtgc acttcctgat gtcagagatg gtctgaaacc cgttcatcgc cgtattttat
     3481 acggcatgaa cgaattaggt gtaacaccgg ataaagccca taaaaaatct gccagaatcg
     3541 tcggagatgt catgggtaaa taccatcctc atggagacta tgcgatctat gagtcaatgg
     3601 tacggatggc acagccattc agctaccgtt atatgctagt ggatggtcat ggaaactttg
     3661 gttctgttga cggtgacggt gccgccgcta tgcgttatac cgaagcacgg atgagcaaaa
     3721 tcgctacaga aatgctgcgt gatatcaata agaacactgt tgatttccaa agcaactatg
     3781 atgatacaga aaaagaacca gttgtactac ctgctcgctt cccgaatctt ttagtgaacg
     3841 gaacaacggg gatcgccgta ggtatggcca cgaatatccc acctcataac ttgtctgagg
     3901 tagtagcggc tatcgatttg ctgatggata atcctgatgt gacgaccaat gaattgatgg
     3961 aagtattacc tggaccagat tttccaacag gtggtttggt tatgggaaaa tcaggaatcc
     4021 gaagagcgta tgaaacaggt aagggttcta ttaccgtacg tgcaaaagtt gagattactg
     4081 agatgccaaa tggtaaagaa cggatcatcg tgacagaatt gccttatatg gtcaataaag
     4141 caaaattgat cgaacggatt tctgaactgc acagagacaa gcgtatcgaa gggatcacag
     4201 atttgcggga cgaatcttcc cgtgaaggta tgcggatcgt cattgacatc cgtcgtgacg
     4261 ccagcgcatc tgttatcttg aataacttat ataaattgac atccttgcaa aattcatttg
     4321 gattcaatat gttggcgatc gaaaaaggtg tgccgaaagt tttgagcttg aaacaaattc
     4381 ttgaaaacta tatcgaacat cagcgcgaag tcatcacaag acggacagtc tttgaaaaag
     4441 aaaaagcaga agcaagggca cacattttag aaggtttgcg tattgctttg gatcatatcg
     4501 atgaaatcat tgcgattatc cgcgggtcta aatcagatga cgaagcaaaa gcgacgatga
     4561 tcgaacggtt cgatttatcc gatcgccagt ctcaagctat tttagatatg cgtttacgcc
     4621 gattgaccgg tttggaacgg gaaaaaatcg aaaatgaata ccaagaactc ttaaaactga
     4681 ttgaagattt aactgacatt ctggcaagac cagaacgtgt tacagaaatc atcaagaatg
     4741 aattagcaga cttgaatcaa cgatttggtg ataaacgacg cacagaatta ctagtcggtg
     4801 aggtcttaag tctggaagat gaagatttaa tcgaagaaga agaaatcgtt attacattga
     4861 caaacaatgg ttacatcaaa cgtttggcaa ataatgaatt cagagctcaa cgaaggggtg
     4921 gacgtggtgt ccaaggaatg ggtatccaca atgacgactt cgtcaaaaat ctggtttctt
     4981 gttctaccca tgatacattg ttattcttta cgaataacgg aaaagtgtat cgggcaaaag
     5041 gatacgaaat tccagaatac gggcgcacag ccaaaggaat accaattatc aatttactag
     5101 gtatcgattc gagtgaaagc atccaggcaa tcattgcggt gacgggagaa gcagaagaag
     5161 gccactatct cttctttacg acgcgtcaag gtacagtaaa acgtacttct gtcactgctt
     5221 ttgctaacat ccgaagtaat ggtctgatcg caattggttt gaaagaagac gatgagctcg
     5281 tcaatgtgct actgacagat ggtcaatcta atatcattat tggaacacat aacggctact
     5341 ctgtcacttt caaagaagaa gcagtccgcg atatgggacg tacagcttcc ggggttagag
     5401 gaatacgcct gcgtgaggaa gattatgtcg ttggggcagc cttaatgaag gaagatcaag
     5461 aagtattagt gatcacagaa aaaggttacg gaaaacggac aaaagtatcg gaatatccag
     5521 tgaaaggccg cggaggtaaa gggatcaaaa cggcgaacat cactgaaaaa aatggtccac
     5581 tagctggatt gaccactgtt tctggagatg aagacatcat gctgattaca gataaaggtg
     5641 tcattatccg tttcaacgta tcgactgttt ctcaaactgg acgttcaaca ttaggcgtac
     5701 gtttgatgaa gatggaagct gatacgaaag ttgtcacgat ggcagcagtc gaaccagaag
     5761 ctgcagaaca aacagttgaa gaacaaggat cagaagaata gccatcaaga ctaattgtgt
     5821 aagctagcaa gaatgaccga ttaaaaaatc tggttattcc tgctggcttt tttgattagt
     5881 tgaatttctc ttaaaatatt cgaaactgtt cattctgtat tgcttttgac caaaatagag
     5941 tgtataatca gagcttgtga gtagtctaat taattagata actctccttg ctcttaggaa
     6001 cctaagagcc gaaagtccac agggaggtga atatcaatgg aaaacacgaa atatgaaatc
     6061 atgtacatta ttcgtccaaa cattgatgaa gaagcaaaaa ctgctttaat cgaacgtttc
     6121 gatgcaatct tgaaagataa cggagcagaa gttatcgaat caaaagattg ggaaaaacgc
     6181 cgtttcgctt atgaaatgaa cggttaccgt gaaggtatct atcacatcgt taaagtatct
     6241 tctccatcat cagcaaatgc tgtcaatgaa tttgaccgtc ttgctaaaat caacgacgat
     6301 atcattcgtc acatgattgt taaagaagaa gcttaattgt tgtttcatgt gaaacaatag
     6361 aaaacgatga gaggagatga ctattttgat taataatgtt gtattagttg gacgtctgac
     6421 gaaagatcca gatttgcgtt acacagcaag tggtacggca gtcgcaacat ttactttggc
     6481 tgtgaatcgt aattttacaa accaaagcgg caatcgcgag gctgatttta tcaactgtgt
     6541 gatttggcgc aaatcagcag aaactttagc aaactatgct cgtaaaggaa cacttttagg
     6601 tgttactgga cgtatccaaa ctcgttctta tgataatcag caaggacaac gtgtatacgt
     6661 gacagaagta gtagctgaca acttccaatt gctggaaagt cgttctgcat ctgaaaatcg
     6721 ccaacaaagc ggcggattcc aaagttctgg tcaatcagct ggcggatttg gcggaaacaa
     6781 taactctaat caaacatctc aatcatccaa cggtatgcct gatttcgacc gtgatacttc
     6841 tgacccattc ggctcaagtt caacgatcga catttcagac gatgatttac cattctaaac
     6901 gaatttgata ggagggaaat agaatggcac aacaaagaag aggcggacgt aaacgtcgta
     6961 aagtagacta cattgctgct aaccatattg aatatatcga ttataaagat atcgaattac
     7021 taaaaagatt tatttcagaa cgcggtaaaa tcttgccacg tcgtgtaact ggtacaggcg
     7081 ctaaaaacca acgtaaaatt actgttgcaa tcaaacgcgc tcgtatcatg ggcttgctac
     7141 ctttcgttag cgaagactaa taaatcctag gaattgaatg actgatcctt ttgatcagct
     7201 attcaattcc tttttttctg taaaaataac ctctattcgt ttagtcgctc gaatgacgaa
     7261 agttatggta aaatcaaatt agtcatatat gggaggaaaa ttatggaaaa taaaaacaat
     7321 catttatccc cgaaacctct cttccttatc ctgtttatat taatcgaagt attggtagtc
     7381 ttaattttac catttcgttt tgttagcgct tcggtcattc tattgatgaa tttatggggt
     7441 gttcgctatt ggttgaaaat gaaacgttgg cttattttaa ataaacgtga aaaaatccag
     7501 caggcttcga ctgtagctga aaccaatcta gcttatgtct cagaagtcat gcctgtagga
     7561 gtgattgctt atcagccatc aagccaaaaa atcgaatggc tcaatccatt agcattgaaa
     7621 atcaaagatc aaagtggatt aacagaggaa gaattgctag atactttttt tactgcaaaa
     7681 gaaaaaaaga aaaaccaagt ctatctagca gataaaacat ttcagtttga tatggataca
     7741 gtaaagggtg ttgtttattt taatgatatt acccaaaacc agttgttaaa actccatcag
     7801 acgaacagtc agccggtgat tggtatttta tctattgata actatgcaga tgccattgat
     7861 aaattggatg acaaagagat ttcttatttg aactctttcg tcactacact agtttctgac
     7921 tggatgaatg aataccatgt ctttttcaaa agaatcaacg cagaacggtt tttcttcgta
     7981 gcacagatga tcgatctgaa aaagatgatg gataaaaaat ttgatttgct ggatcgcttt
     8041 cgagcagaag ctgaaaaaca gagctcgcct atcaccctta gtatggggat tgcttatggc
     8101 tcaagaaccc tcgatgaaat cggtggagaa gctcagaata atttagatgt tgctttagtc
     8161 cgtggaggcg accaagttgt cgtgaaagag gtaaaagaag gagcaaaacc tgtttattat
     8221 ggggggaaat ctgctaagaa tgtcaaacgc acgcgtgttc gttctcgagc gatgagtacc
     8281 gctttgcgtc ggatttttga tgaaacaggc gatgtgttta tcatggggca caagtttccg
     8341 gatatggatg ctttaggctc tgcttttggt gtcagttgtc tggcacgctt caaccagaaa
     8401 aaagcttaca tcgttatcaa cgagaacgag atcattccag atgtagaacg gtgtcttgaa
     8461 gaaatccata agcatcctga attagaaatg caactaatct ctccagaacg tgccatagag
     8521 ctgaaaaaag aagaggacct cctcgtgatg gtggattacc ataaaccttc tctatcgatc
     8581 tctcagggcc tatatgagca atttgataag attgtgatca ttgatcacca tcgtcgtggg
     8641 gaagagtttc caagtaaacc tctgctgact tatatcgaat cttcagcttc ttcagcttca
     8701 gagctagttt ctgagctgat ccaataccaa agcagcaaaa caaatcgctt agatcgtttt
     8761 gaggccacat tattgcttgc agggatggtt gtagatacaa atagtttcaa tgtccgtaca
     8821 actgcacgaa cttttgacgt tgccagctat ttaaggtctt gtggagcaga cgcatcctta
     8881 gtacaatatt tattgagctc ggatttagcg acatacttgg agatcagcaa tttgattgct
     8941 ggaagcgact atgtgacttc tgatattgtt gtggcggcag ggacagacga aaaagagtat
     9001 aatagtgtta tagcagcaaa aacagcagat acattgcttt cgatgatcaa tatcaatgca
     9061 gctttcgtca ttacaaagcg gacagacggc ttgattggga tcagtgccag aagtaccggc
     9121 acgatcaacg tccaaatcat catggaatcc ttaggtggcg gaggacactt cacaaacgca
     9181 gctactcaac tggaaaatac gacagttgag gaaacaaaac agaagctatt tgctgcaatc
     9241 aaagaaaata tgaatcaaat atatgataag gagtaatcca aatgaaagta atttttttac
     9301 aagatgtaaa aggaaaaggt aaaaaagggg acatcaaaga agttccagca ggttatgcac
     9361 aaaattatct aatcaaaaat aacttggcaa aagaagcaac gaaaagcagt atcagtcagt
     9421 tagcaggaca aaagaaagca caagaaaaac acgaagctga aatcttggaa gaagctaaaa
     9481 agttaaaaga attcttggaa aaagaagata ctgtagtcga attgaaggcg aaagcagggg
     9541 aagacagccg tttgtttggt tcgatccctt caaaacaaat tgctgaaggg ttaaacaaac
     9601 aatacaaaat caaattagat aaacgaaaaa tcgaattaga aaatccgatc cgttcactag
     9661 gctacacaaa cgtacctgtt aagcttcatc atgaggtgac ggctacgatc aaagttcatg
     9721 tcgtacaaga ataacaaatc ttgatagcga tgttcagctg gtaagtataa ccagaaggaa
     9781 aaaagacaaa ccgaaattta tctgagaaaa cagctgcagc aatgaaagca aaagctgcaa
     9841 atcgatttca gattgatttc ggtttctttt ttgggaacag caaaatagca taaagaaaac
     9901 gagtataata aaagagaact atttttttat tacactttcg gtacgaatga tgacttttct
     9961 ctcattttct tgtaaaatga agcaattgaa gacaaggagc gatacgtatg aatgaagtgt
    10021 ggcaggatcg aatcccaccg caaaatattg aagctgaaca agcggtttta ggctctgttt
    10081 ttcttgacgc agaaacgatc attgatgcca tggagtacat agagccaaaa gacttttatc
    10141 gtcgtggcca tcaaattatt tttgagacaa tgatcgagtt aaatgatcgt aatgaagcga
    10201 ttgacgtggt tactgtcaaa gaccggctgg aacaagccaa tttgttagaa gatgccggtg
    10261 gtttaagtta tctctcagac ttggcattag cagttccgac agcagcaaat atcgtctact
    10321 atgcgaaaat cgttgaagaa aaatcattgc tgcgtacatt gatccagaca gcaacaggaa
    10381 tcgttacgaa aggattcgag caaggagaag aagttcaaac aattttagat gatgccgaac
    10441 gcagtatctt ggaggtttcg gaaaaaagaa atcgaagcgg ctttttatct attgctgaag
    10501 tactgaactc cacgattgaa aatatcgatc gactagctca aaacaatgaa gaaatcacag
    10561 gtctaccaac tggctatcaa ggtctggata aaatgacagc aggtttccag cccgaagaac
    10621 taatcattct tgctgcacgt ccagcggtag gtaaaacagc gtttgcattg aatattgctc
    10681 aaaatgtagg aacaaaaaca gatcgttctg ttgcgatatt tagtttggaa atgggggcag
    10741 aatctcttgt aaatcggatg ctttgtgcag aaggttcgat tgaggccagc catttacgta
    10801 caggtcagct ttcggaagaa gaatggtcga atctgatcgt tgcgatgggg agtctttctc
    10861 gagcaaatat ttttatcgat gatacaccag ggatcaaaat ctctgaaatc cgtgcgaaat
    10921 gtcggaaact agcgcaagaa aaaggcaatc taggcttgat attgatcgat tatttgcagt
    10981 tgattgaagg taacggtaaa gagaaccgtc aacaagaagt atcagatatc tctcgccaac
    11041 tgaaaaaact ggcgaaagag ctgaaagtac ctgtcattgc tctttcacag ttatctcgtg
    11101 gagtagaaca gcgacaagac aaacgaccag tgctaagtga tattcgtgaa tccggatcaa
    11161 ttgaacaaga tgcggacatc gtagccttct tgtatcgaga tgattattat gaaagagaag
    11221 gcggagatga cgatgatgga aacgagccaa aaaatgacaa cgtcatcgaa gtcatcatcg
    11281 agaagaaccg aagcggtgca cgaggaacag ttgaactact gtttatcaaa gaatacaata
    11341 agttttcgtc gctttcgcca agagaagaat tttaaagaag aggttgtgag ccttgagcaa
    11401 tcccgagagc tctcgtaaga agatgcaagg agtaaccgca ggaacgacta gagtacttaa
    11461 tttaaacaga tctccaaagg gattgtgaca aaagtcttgt cacagtccct ttttccgaat
    11521 aaacggtgtt caatcagctg acccttggta ttagctcagc aaacgtcaaa atatgaggaa
    11581 ctattttgac gtttgtttct ctaatactca ggtcaaaacg ctgatcttac gacctctttt
    11641 ccttaaacgg tgttctcctg ccaactcctc ggtcacaagt cacaatatca tcaaacatga
    11701 gaagcagttt tttgaaattg ctccttgtga ctcagagtta aacggcaggc tcacaacctc
    11761 tttcttaaac ggtgttcaaa agcttgcatc tgcggtaata cgcccaatca aacgaaaaat
    11821 atgagaagct atttttgttt ggctgttctt attacttgat gcaggacagc tttttcacaa
    11881 cctctgattc acggtgttcc aaaagtagct tcttcgaaaa ataagtcgaa atttcacaaa
    11941 aatttgagaa gcaattttcg tacattcctt cttatttctt gaagctgacc acttctgtta
    12001 caacctcttt tttaataacc attctaaaaa gataaagttc gttttttaag cgtttttata
    12061 aaataaaaat aaaaatcatt cgattttatt tgaaatatgt atctattatt gatacaatgg
    12121 ttcaggatta aataatatag aacgaggtgt tcacatgtca tctgtagtag tagtaggtac
    12181 gcaatggggc gatgaaggga aaggaaaaat cacagacttt ctcagtgaaa atgctgaagt
    12241 gattgcacgg taccaaggtg gcgacaatgc agggcacaca attaagttta acggtgtgac
    12301 ttataaactg catttgattc cttctgggat tttttataaa gacaagatca gtgtgatcgg
    12361 aaacggcgtt gtagtcaatc ctaaatcatt ggttaaagaa ttagcttatt tacatgaaaa
    12421 aggtgtagaa acaagtaatt tgcgtatttc tgaccgcgcg cacgtaatct tgccttatca
    12481 tatcaaattg gaccaattac aagaagatgc taagggcgaa aataagattg gtacaacgat
    12541 taaaggaatc ggacctgcct atatggataa agcagctcgt gtaggtatcc gtattgcaga
    12601 cttattagat aaagaaattt ttgaagaacg tcttcgtttg aatctagatg acaaaaaccg
    12661 tcaatttgta aaaatgtttg attcagaacc acttgatttt gacgatattt ttgaagaata
    12721 ttatgaatac ggacaacaaa tcaaacaata cgtcacagat acttccgtta tcttgaatga
    12781 tgctttggat gcaggcaaac gcgtgttgtt tgaaggcgca caaggtgtaa tgctggatat
    12841 cgaccaggga acttatcctt ttgttacttc ttctaaccca gtagccggtg gggtaacaat
    12901 cggaagcggt gtagggcctt caaaaatcaa taaagttgtc ggcgtctgca aagcctatac
    12961 gtcacgtgtc ggtgatggac catttccaac tgaattattt gatgaaacag gagaacaaat
    13021 ccgcgaagta ggacgagaat acggtacgac aactggtcgt ccgcgtcgtg taggctggtt
    13081 tgattctgta gttatgcgcc attctaaacg agtttcaggt attaccaacc tatctttaaa
    13141 ctcgatcgat gtattaagtg gattggaaac agtaaaaatt tgtacggctt atgaattaga
    13201 tggcgaacta atctaccact atccagcaag cttgaaagag ttgaaccgct gcaaaccaat
    13261 ctatgaagaa ttgccaggct ggtcagaaga cattacagga tgcaaaacac ttgcagaact
    13321 gccagaaaac gcacgtaact atgtacgtcg tatctcagag cttgttggtg tacgtatctc
    13381 aacattttct gtcggacctg atagaacaca aacaaacatc ttagaaagcg tctgggcgca
    13441 aatctaagga taaaaacgac gactgaatca ggacgttctt accagagcga gtcgaagtgt
    13501 ttatctatac gataataata acttgaacac tgtttattca gaataaggga ctgtgacaaa
    13561 aaccgtgtca cagtcccttt ttccgaa
//
LOCUS       DAKBVG010000066        13578 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000066 DAKBVG010000000
VERSION     DAKBVG010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.066"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            10..522
                     /locus_tag="OUD09_001319"
     CDS             10..522
                     /locus_tag="OUD09_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003243190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HCT9692596.1"
                     /translation="MEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMVKGRWVK
                     DQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPEITVTFQ
                     NEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPDDIRSGK
                     YFDENYKRVK"
     gene            complement(1005..1448)
                     /locus_tag="OUD09_001320"
     CDS             complement(1005..1448)
                     /locus_tag="OUD09_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCT9692597.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(1464..2624)
                     /locus_tag="OUD09_001321"
     CDS             complement(1464..2624)
                     /locus_tag="OUD09_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCT9692598.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            3131..4831
                     /locus_tag="OUD09_001322"
     CDS             3131..4831
                     /locus_tag="OUD09_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9692599.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(4933..5766)
                     /locus_tag="OUD09_001323"
     CDS             complement(4933..5766)
                     /locus_tag="OUD09_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCT9692600.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(6070..8235)
                     /locus_tag="OUD09_001324"
     CDS             complement(6070..8235)
                     /locus_tag="OUD09_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCT9692601.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            8495..10789
                     /locus_tag="OUD09_001325"
     CDS             8495..10789
                     /locus_tag="OUD09_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HCT9692602.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            10782..11447
                     /gene="pgmB"
                     /locus_tag="OUD09_001326"
     CDS             10782..11447
                     /gene="pgmB"
                     /locus_tag="OUD09_001326"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCT9692603.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            11440..12447
                     /locus_tag="OUD09_001327"
     CDS             11440..12447
                     /locus_tag="OUD09_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HCT9692604.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            12469..13488
                     /locus_tag="OUD09_001328"
     CDS             12469..13488
                     /locus_tag="OUD09_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCT9692605.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
BASE COUNT         4118 a         2808 c         2468 g         4184 t
ORIGIN      
        1 tttaaagaat tggaagattt tattggaact cactttatct atacgtatga caatggttgg
       61 gaatatgaat ggtatgccaa aaatgaccat actgttgatt accgtatcca tggcggaatg
      121 gtcaaaggcc gttgggtaaa agaccaagaa gctgatatcg ttaaattgac ggacggcgta
      181 ttcaaaatca cttggacaga gccaacaggg acagacgtgg cattggactt tatgccaaat
      241 gaaaagaaac ttcacggaat gatctttttc cctaaatggg ttgaagaaca tccagaaatc
      301 actgttactt tccaaaacga acatatcgca gaaatggaag cagctcgtga aaaatatgaa
      361 acttatccaa aattacttgt tcctgaattt gctacgatca cttatatggg tgatgcaggt
      421 atagacaatg aagatgttat tagcgaagaa ccatacgatg gaatgccaga tgatatccgt
      481 agcggaaaat acttcgatga aaattataaa cgagtaaaat aaggttgtga aagagaggtt
      541 gtgaaatcag cgttctgacc tgagtattag agaaagaaac ggaaaaatag ttcctcatgt
      601 tttgccgttt tttgagctaa taccgagggt cagctgattg aacaccgttt atcaagaggt
      661 tgtgaaatca gcgttatgct ttgagtaata agaacgctag ttagaaaata gcttttcata
      721 ttttctaggc tagtgggctt attaccgaga agcagccgtt tgaacaccgt ttatcaagag
      781 gttgtgaaat cagcgttttg acctgagtat tagagaaaca aatggaaaaa tagttcctca
      841 tattttgccg ttttttgagc taataccgag ggtcagctga ttgaacaccg tttatcaaag
      901 attgtaacaa ctgcatattc acacagttaa attagtgact tcaaaatgaa ataatgctcc
      961 cagcaaaaaa ttaggctggg agcattattt cgttattctg ttcatcaaaa atcagcgttt
     1021 tgattccatt tcatcacaag ttcctcctca tttactgctt catctttttg ctgtgtttga
     1081 attgtaaaac cttgtgaaag atagaaatga atagctgatt gatttttttg atagactgtc
     1141 aaagtcagtg ttttattggt ttcttttatt tttttcatta gttgcgtacc gattctttgt
     1201 tgccggtatt cccttttcac aaagattcct gcgataaact gatcaatcac cccagcaaaa
     1261 ccaataattt cttgatctat ttctgctaag taaatcactg catctggtaa agctttccta
     1321 acagattcta cttgattctc ccaataagtt gatggaatga attcatgaga atccaagtta
     1381 gcatgcagcc aaatcgatag aacagattcc agttctttgg cagacaattt tgttgttgtg
     1441 cggatcattt tttctctcct tacttattca ttgattttaa aataaaaacc atttttttga
     1501 agctctttta caagttttcg cccctcaaaa aagtatatct tctcttgttt tacttttcta
     1561 ataccaaaag aaacagcaac tttaggatca tcagctgaaa cgattcgata agttccacca
     1621 ggcaattgat ccagcttgac tttcccatga tggtcgcttt gtactgaaaa cggcacacca
     1681 tttctagtaa gtgttttttt cttatgtttc aaaatgaatg aactaccatt taccggtttc
     1741 cctgacgaat cactaataat aaatgaaaaa tcaagcactt ttttacttaa tctatactga
     1801 tagattagtc gtccgataaa cactaacagt atgactacag ctattatcgt tcctgcaaga
     1861 ataaaccatt gccgcatggt tctttcctga tttccttttc cttcttcttt ttttaccgat
     1921 tcgttataag gaatccgatg tcctgtcact agcaaacgat gcgtgttgat catatacggc
     1981 gtacatgtaa gcaaggtaac taagtcttct ccttctgaaa tcgttagaac agacgtgtca
     2041 tctggcagta ccacttgtat atttttaact tgataagcca atttctcccc caaaacagtc
     2101 aaaataaacg tgtctccgtt ttctaaacga tctaattcgg taaataaccg tcgattaggt
     2161 aaaccgctgt gagcagagat cactgaatgc gtcgaatttc caccaagcgg gaaagaggtt
     2221 ccttgcaaga cgcctgctcc attttcaagg atcctaccat tgagccgatc ataaagtgaa
     2281 atatttaatt ggatcttagg tatattgata cttccgatca gccattcctc taattgactg
     2341 gatgttgtac tttcatttct gttcttttca ttgaaaggat cagtaccagg attgaatcct
     2401 tggtcagtta atcgttggtt tttctcctcc attttctttc gctgcacttc aatttcagct
     2461 gcggtccgtt tttgtacttg ttcgatacgt ttttgatcaa taaaactatt taaagaatcc
     2521 acataaaatg gatataaagc gatcagtaac cctacgacga tacatattaa taaaaacaaa
     2581 cgaaacaaaa gtcgacgaga ctgtttttgc ttactctgct tcacatgtgt cctcccttgc
     2641 aagttttttt gttctttctt ttattatatg ggttcaggaa aaaaatagaa aatgaaagcc
     2701 gatgaggttg tgaaaaagct gtcctgcatc aagtaataag tacagcccaa aaaaatagct
     2761 actcatattt tttgttgggt tgggcttatt accgcagatg caagcttttg aacaccgttt
     2821 aaggaagagg ttgtgaaaac tgcgttatgc tttgagtaat aagagcgcta gtcagaaaat
     2881 agcttctcat attttctagg ctagtgagct tattaccgag aagcagctga ttgaacaccg
     2941 tttaccgaga ggtcatgaaa aagctacttt gtttcgagta ttaagaaaaa ttctgtttca
     3001 aaacaaggag atgcaacaaa acttatgtcg catcctctct ccttagaact ttcacttatg
     3061 tttttcttgt aaaattcata aatattttca tgaattttct aatttcttgc ctacctctcg
     3121 cataggatat atgatgaatt tatctaaagt ttgcaaagga gttcgacaat tgaaaaaata
     3181 tggcccattt ttagcaattt ttttcattgc tttggcattt ctcatgcctc atttattcac
     3241 acataaacta attttgggtg ctgatgcccc attccattac aatcggttct atgatgccgc
     3301 gcaacaaatt aaatatgggg atttccatta ttttccttcc ctttatggat tccagcaaag
     3361 tggcaggatc gtgaatgccg tttacggccc atttttcgct tatttacaag gattgatcgt
     3421 gttgcttgca ggtagctggt ttcattatca gattctttca aatttcattc tttatcttat
     3481 ttcaggattt tctatgctga ttttactgag atatgtcaat gttcgaaatt gggtaagtgt
     3541 accagtagcc gttttattca tgagtacgta ttctattcaa tactggtcta tcaaccaagg
     3601 attcactagc tgggggactt gtttttatcc tttatgtatg atccctattg ctgatttcgt
     3661 ccttcataaa aagttccctg ttatcaaagt cgctgtctca gtgggactta tgacacagat
     3721 ccatttactg tcttcgatca tgttagcttt gatgtatatc cctttttatc tttattactt
     3781 ttttaaccag actgaaaaag gaaaagcact gcttcaactg attaagtcgg tcttgcttta
     3841 ttttgttctg acagcaaata tttgggcagg attagctatt gtttacagtg ggaataagat
     3901 tcttcctccc tttgttaaca gagaaatgag tcaaaaggcg atagatctgg atggcagtta
     3961 ttggctccat taccctaagg cctttcctat attgatatta tcaggttttt tgctttttat
     4021 tttctttcaa caaaagaaca ccttattcca aaaaatcgtg ttgtacacaa gtcttgtttt
     4081 cttttttctt tctactagct tgattccatg gacgacattg atcgaaaaaa atgtgccttt
     4141 tattagtttg atccagtttc catttcgatt tttcgtgcca tttacgctat tgcttctact
     4201 ttaccttgcc ctttcactga accagtggtc tgaacaaaag tggttccgtc ttttaagtat
     4261 atttggcatt cttatttttt cagcacagac gatccagaac ttataccagc atctggaaaa
     4321 gtgggaaaat gaaacatttg tcagtcgaca tacttatcta ttcgatacgc cggaagaagc
     4381 aaggaaaaca ttcttcagca aagatatgtc taccagcttg ttcgttttcc aaaaaaccac
     4441 acctgactat ctgccaatct ataaggagac aaaagacaat aaatacgacc ggtataacga
     4501 agaaatacta gaaaacgaaa caacttttat aaaaacacat ccaggcggtc ttttgacaat
     4561 caagtggaaa aacactgggg acaaaactgt tcatatccct gttatcgtct acgatcggac
     4621 tgtgctacag caaaatggaa aaacattgac tgattatgaa gtaacagata tcgggacccc
     4681 aatcgtgaag cagcaaaaag gaatcaacga gctcacgctt cattatcaaa cgccaatcta
     4741 tttctatttc attctttctc ttactttgat tggctggttt accttgctat gtttatttat
     4801 ttatcataga tacaagcttt tacgtgctta ggcaaaaggg ataaatacat ccgatcaaaa
     4861 aactctccaa acggtataat cgtttggaga gttttatttt aatcagtttt tttatgttat
     4921 atgttatatt ttttattcgt tcacatctaa ttcgatttcc accccgtagt gatcactgat
     4981 gattggacta tcgatcccat caaaaatccg ttgatatttt ttaacctgca ttccttcagg
     5041 gacgaaaata aaatctatac gcaatttttc tgtattttgt tcccaaccat ctattttctt
     5101 ttccacagta gctgcttttc ctttttcatt cgcgacaaca tacgaatctt gaagaagaag
     5161 gcaactttct cttacagtat aataaccttg agagtctact ggattattga aatcacctag
     5221 gaaaaaaagt ggtacgcgag tttccaaaaa gtagttttcc aagtttttcc actcaaaata
     5281 aaagccactt tttttatctg tccaccaaga aaagtggcag ctcgagacaa aaatgtcctg
     5341 atcatctatc tttgttttcc caatcaagat cttgcgtgtc tgataatctt ctttcttttg
     5401 aaattgggag acggtaagga cctgactttc caatgttttt ttcgtaagca gtgcatttcc
     5461 ttcttcatat ttatcataac cgatgtggct catttcccaa ctccaaaaat actcttttcc
     5521 gtgttccttt aaaagctgaa ctaaacggta ggcaaaatta tctttacgga taggtacttg
     5581 attctttacc ggacagaact tcattagctt cgcaggttct acttcttcag attccagtaa
     5641 ttggttgatt tcttgtaaag caatcacttc ataatcttct aacaggatct tatctgctaa
     5701 ttcttgcaat tttttctctg gttctggctc aagccagcta tgcgtgttga gtgtcatgaa
     5761 tttcattggt tttctccttg tcttcatttc gaaacaaaag ggttgtgaca aaatttttgt
     5821 cacaacctct ccagcaaata gtgttctcct gctaactcct cagtaataag ctcaatggac
     5881 caggagctat tttttgtttg gctgttttta tcacttggtg cagggcagct ttttcacgac
     5941 ctctccacta aacagtattc aaactgctgc ttctcggtaa taagcccaac caaacgaaaa
     6001 ataggaggag ctatttttgt ttggctgttc ttatgacttg atgcaggaca gctttttcac
     6061 aacctctcct tacattttaa tggagccgat cactttacca gctgtcactg tacctaattg
     6121 cttgatcact acttcttcta ctttatcgtt tgtaaatacg atcatgatcg ttggatcttt
     6181 ttcttcgttt tggatcacat caagatccat ctcagctaat ttcgttcctg ttcctacttc
     6241 ttggccttct ttgacagcaa tctcaaaagc tggctgattc atttggactg tatcgattcc
     6301 catatggatc aaaacttcga taccatcttt cgtttccagc ccaattgcat gtttgctagg
     6361 gaaaatactt gtcacttttc cagcgatcgg cgcaacaact tctcttgatg ttggaataac
     6421 agcaaagccg tcacccatca ttttttgtga gaataccgga tcttttactt cagataaagg
     6481 aatcacttct ccagaagctg cggaaactag ttcttgttct atacctaagt attgcttact
     6541 acctgcttgt ccatttcctg tagaaaccat ttctggctct ggaatctctg ctcctgattg
     6601 cagcagatct tcgatatctg atttcaagac atctgctttt ggtccataaa ctgcttggac
     6661 tccattgtct ttgacgatca atcccatcgc ccctgctcgt ttccaggctt cttcagtccc
     6721 tactttttct cgatcattta cacttacgcg taaccgagtc atacaagcat cgacatcttt
     6781 gatattttct tttcctccca gcaaatgaat gatctggacc acttgctgat ctgctgttcc
     6841 tgttccgctg ttagatacaa cggtttcttc actgtcattg tcgtaatttc cgttacgtcc
     6901 cggtgtagca aagttgaatt ttttgatcaa gaaatttgct aagaaatacg taacaacacc
     6961 aaaagcgatc acacacaaca cgaagttgat aagatctcca cctagacccg ctttgatagc
     7021 taacggtgtc cgagtcagca attcgatatt cccaaatgaa tggacccgca atggcaagat
     7081 atctgccatc gcaaatgcag ccccttggat cactgaataa atgacatata atggcacagc
     7141 agcaaacata aacatgaact ccaatggttc tgttacacct gttagaaaga cagccaacgc
     7201 tgctgaaaaa tacattgatt tatatcttgc tttcttatca ggatcgacat tacgatacat
     7261 cgcaaaagcc attcccatca agatacctga agaaccaatc atttgtccta ctttgaaacg
     7321 agccggcgtc cagttatgca acacaaactc atattgcgac atatcccctg cacctttaag
     7381 attcactaaa tcagtggccc aagccaacca taacggatct tgcccaaata cttgtgtccc
     7441 cgcctgtgcg cctgataaaa tctcatacga tccgccaagc tgtgtatagt tgataggaat
     7501 cgtcagcata tgatgcaatc caaatggcaa tagtaaacgt tctaatgttc caaataaaaa
     7561 cggtgctaga atcggtgcgc tttcttgtga ttctgcaatc cataaaccaa aattattgat
     7621 tccagcctgt acgtttggcc acaccacagc aagaatcaat gccacgattg ttgaccataa
     7681 gataacaaca aacggaacaa agcgctttcc attgaaaaat gacaaagcat ccggcaattt
     7741 tctataatta tagtatttat tataagccat tgccccgaca aatccagcga tgatccctac
     7801 gaatactccc atattcaaag ctggtgcttc caatacactt gtaaaaaatc ctttaaccat
     7861 gatcttcgta ccgaacagag tgtgggtaaa tgcatcttca ttcgcaagca tgtcacttgt
     7921 gacaccaaag atagccccag tgatccgatt gattaaaata aatgagatcc cagctgcaaa
     7981 tgctccgcct gctctttctt ttgcccaact tcctcctatt gctaaagcaa ataaaatatg
     8041 aagattacca ataattgccc aaccaatatt ttcaattact ccgcccgttg tgacaagcgg
     8101 cgtccaatca ggattgatca agggaattga tttccctata ctgatcatca acccagctgc
     8161 cggcataact gctaccacaa ccattaaagc ctttccaaat ttttgccaaa attcaaaact
     8221 aaatatcttt ttcattccgt ttagctcctt tacgcaatcg gtttcgttaa acgtattata
     8281 gcgtgcgttt tcttttttgt aaatgcttta ttttttattt ttctgtctat accattctat
     8341 aaaacctata atacgtgtgt tcccttctat aattttctcc taaaaattta tttttttaag
     8401 cttttttcta tgaaatattt tcttttgtct attgacttgt gcaatcggtt gcgtttatta
     8461 tgaacgtata aaacagctca ttaggaggag aaaaatgaaa cagatcaaac gactatttgc
     8521 tattgatcca tggaaaatca gcacgacaca attagataaa aaaaatcttc gactgcaaga
     8581 atctttaaca agtattggaa acggttatat ggggatgcgc gggaactttg aagaaactta
     8641 ctcaggggac caccatcaag gtacttattt ggcgggtgtc tggtatcccg ataagacacg
     8701 tgtcggctgg tggaaaaatg gttatccaga atactttgga aaagtgatca acgccttgaa
     8761 ctttatcgcg atagatattt cgatcaacga tcagctgatc gacttggctt ctttggaacc
     8821 agaagacttt tactgggaat tagatatgaa aaatggtgta ttatctcgta cattcacaat
     8881 cactacttct actaataaag tacgcatttc cttcgaacgt tttctgagca ttgtcaaaaa
     8941 agaatcagct tatatctatt taactataga aatgcttgaa ggtagcggaa cagtcaaact
     9001 cgtatcaaaa ttggatggaa atgttcaaaa cgaagacagc aactatgatg aacatttttg
     9061 gaatgagatt gatcgtggaa ctatagaaga aatcggctat ttgacgacta aaactattcc
     9121 aaacaacttt gggattgaac aatttactgt tactgcagca atgcgccatt tcgttaatgg
     9181 aaaatcagtc atccctgact atcacgaaga tactttagca ttacatggtt gcttgtctgc
     9241 agaattatct caaggtgaga catggtcagt ggaaaaagaa attatcgttc ttactagtag
     9301 agatattcca gaagcgcagc aagcagaaaa agctgtctca gaattaacga accagacaaa
     9361 caattatatc caagcaaaag aagagcaaac agcggcttgg gcagaacgat ggaagcttgc
     9421 cgatgtggtc atcgaaggag acgaagaagc acaacaaggt attcgattca atttgttcca
     9481 actattctct acttactacg gcgaagatga acgattgaat atcggcccca aaggattcac
     9541 aggagaaaaa tacggcggag ccacttactg ggatacagaa gcgtatgctg tccctcttta
     9601 tcttgcttta gccaaacctg aagtaactaa gaatctgctg aaatatcggc ataatcaact
     9661 gccgcaagca atccacaacg ctcagcaaca aggattaaaa ggcgcgcttt atcctatggt
     9721 caccttcact ggtgtcgaat gccataacga gtgggaaatc acattcgagg agattcaccg
     9781 aaatggcgcg attgcctatg caatctacaa ttatacgaat tacaccggcg atacggaata
     9841 tttgaaaaat gaaggactgg aagttttagc agaaatcgca cgcttctggg cagaccgtgt
     9901 ccatttttca aaacgagcaa ataaatacat gatccacggt gtgaccggcc caaacgaata
     9961 tgagaacaat atcaataaca attggtatac aaatacgatc gcagcttggg tcttgcgtta
    10021 cacgcgagaa aattatttaa cttatcaaaa agaagctgct atcactatca gcgaagaaga
    10081 actagccaaa tggaaagata tcgcagagaa catgtacttc ccagttgacg aagaattagg
    10141 aatctttgtc cagcatgaca cattcttaga taaagatctc atgcctgtat ccgatattcc
    10201 cacaagcgat cttcctctga atcagcattg gtcttgggac aaaatcctta gatcttgctt
    10261 catcaagcag gcagatgtac tacaggggat ttatttcttc aatgatactt ttacaaaaga
    10321 agaaaagcaa aaaaactttg atttctatga accaatgact gtccatgagt cttccctttc
    10381 gccaagtatc cattcgattt tagcagcaga attaggaatg gaaagcaaag ctgtagaaat
    10441 gtaccagcga acagctcgct tggatttgga taattacaat aacgacacag aagacggctt
    10501 gcacatcact tccatgactg gtagctggtt gtcgattgtc caaggatttg ctcaaatgaa
    10561 aactgcccat gatcagttga gattcgcacc atttctgcca aaaacttgga aagcttattc
    10621 tttccatatc aattatcgcg gacgtttatt gttcatctca gtcacagatg aacaagttac
    10681 tttcgagtta ttagaaggtt ctcctctaga aatttacgtc tatgaacagt cttattcttt
    10741 agcggatcgt ctagaaataa cgctaagaaa ggaggaagca aatgtttaaa ggggtattgt
    10801 ttgatttaga cggtgtcatt accgatactg ctgaattcca ttatcgtgct tggcgaaagc
    10861 ttggtcagga aatcggcatt acgatcgacc acacattcaa cgaacagcta aaaggcgtca
    10921 gccgagaaga ctcactctct ttgcttttag cttatggcgg aaaagaacac tctttttcaa
    10981 aggaagaatt tgcagaatta gcgaaaagaa aaaacgatta ttatttagag atgatccaga
    11041 cgatcgaacc aaaagatgtc ttccccgggg tcgtgccttt actggattct ttaaaagaag
    11101 aaaaaatcaa gattgctctt gcttctgcaa gtaaaaacgg gccattccta cttgaaaaaa
    11161 tgggtcttac accttacttt gatgctattg ccgacccggc agaagcaaca aacggaaaac
    11221 ctgcgccaga tatcttcctc cttgcagcaa aagctgtcgg actaaaagca gaagaatgta
    11281 tcgggatcga agacgcgcaa gccggtatcc aagccatcct ctcaagtggc gctcaaccaa
    11341 ttggcgtggg acgtgctgaa gaccttggag aggagatccc gcttgtccct gatacgactt
    11401 ttcttacgat cgattacttg aaagaaaagt ggcatgacca tggataatcc agagatctcc
    11461 gtcgtaccag ttccagagac tgaaataaag aaaatcacat tgaagaacga ctacttagaa
    11521 gtcgttcttc tcaattacgg tgcccgcctg caccaaatct tcgcaccaga caaagaagga
    11581 aaatcagaaa atgttttatt gtcttacgat cgtttcgaag atgttctaac agacaaaagc
    11641 ttttttggag caactgtcgg acctgtagcc ggaaggatcc gggatgcttc ttggggtgag
    11701 catagattgg agaaaaactg cggatcacac catatccacg gtggcacgaa aggctggtct
    11761 ttccaatttt gggaagtaga agttttcaaa actccatcat caataggtgt ggtcttttac
    11821 ttaaaagatg aattttctac ctatccagga ccaatcactg ctacgatcac ttaccgttta
    11881 acaaaaaacg aattagagat gacaacctct gctagtagcc ttgttgaaac catctgcaat
    11941 ccgaccaacc atgcctactt caacctttca ggaaatggaa caagagacat ctatgaacat
    12001 cagttaacgg tctttttaga tggtgtgctt gaattagatc aagaaaaact gcctactgga
    12061 aattggacaa aaaaagaaaa actgccaatc gactttagaa aatccccaac actgcaagag
    12121 atccttgcat gttatccaga tggactagat gatgtatttc ttttgcatca tcctaggctt
    12181 tccaaaactt ccttacaatt attcgagaag cattctggtc gccaaatgac tatagcaact
    12241 tctaataaaa gtatggtatt attttctacg actggattcg aagctgattt ctctgtcaat
    12301 ggaaaacaga tgcattcaaa ctacggatta gcaatcgaac cgcaagaaat ccctgatatc
    12361 gttcatttcc ccaaatttgg atcgatcaac ctccatcctg gtcaagaacg gatcagtcag
    12421 acgatttatc ggttctctgc acaataacaa gagaaaggtg tttttatcat ggctattacc
    12481 gtaaaagatg tggcgaaaaa agccggtgtt gccacctcga ccgtctctcg tgtcatcaac
    12541 gatcatccta gtatttccga gacaacaaag aagaaagtcc gaaaaattat ggatgaacta
    12601 ggttatgttc ctaacatgac tgcccgcaat ctaggaaaac gcatttccag tgccatcggc
    12661 gtaatcttac cccctcttga ttcaaaagag cggatcggta acccatttta tcttgagatc
    12721 atggaagcaa tcaacgaaga agcgcggaat tatgatatga ctacagcaat cgctactgct
    12781 aaaaattttg aattgctgtt agagaacgtc cagcgtatgc atttacaaaa acaagtagat
    12841 ggttttattc tagtgtattc agatagtcaa gatactgtca ttgattattt ataccaaaac
    12901 cacatccctt tcactttgat cggacagcct tatcatcatg agaacgaaat cgtttatgta
    12961 gataatgata accagcttct aggtaaacaa gcaacagaat ttttgatcca gaatggacat
    13021 aagaatatcc tgtttgtgac aaacacgact catgaaagtt tgtatttcga acgatatttc
    13081 ggttatcaaa aagcaatgat gatggctggt cttcctgcgt tatccagtgt agtactagag
    13141 catccggagg attatctgtc ctttgagaaa acgttgaaag aaaacgaagc cacagcattg
    13201 gtggtcattg acgatatctt cgcccttcgc acgatgcagt tggcgcaaat gtatggctat
    13261 gtcattccag agacactctc cgtcattagc ttcaacaact cgatcttctc aacattaaca
    13321 cacccttacc ttacaagtat cgatatcgat atctccgaat taggacgtgt cgctatgcaa
    13381 aagctgcgcg aattgatcca tgaagaattg agcagtggcg tacgtttggt catccctcat
    13441 cgcttgatca aaagagagac agtcgttcct ttagaaaacc catactaaat agctgactca
    13501 ctaaatacgc tgaccattta tcctatggtc agctttttat gtaaaagaag atactacttc
    13561 taacacattg tttatctg
//
LOCUS       DAKBVG010000067        13232 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000067 DAKBVG010000000
VERSION     DAKBVG010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.067"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            36..335
                     /locus_tag="OUD09_001329"
     CDS             36..335
                     /locus_tag="OUD09_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9692606.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(432..866)
                     /locus_tag="OUD09_001330"
     CDS             complement(432..866)
                     /locus_tag="OUD09_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCT9692607.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1098..1808)
                     /locus_tag="OUD09_001331"
     CDS             complement(1098..1808)
                     /locus_tag="OUD09_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCT9692608.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(1798..2658)
                     /locus_tag="OUD09_001332"
     CDS             complement(1798..2658)
                     /locus_tag="OUD09_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HCT9692609.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(2663..3301)
                     /locus_tag="OUD09_001333"
     CDS             complement(2663..3301)
                     /locus_tag="OUD09_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCT9692610.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3316..3615)
                     /locus_tag="OUD09_001334"
     CDS             complement(3316..3615)
                     /locus_tag="OUD09_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCT9692611.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKEVTVIGIKNVMSAKEISEKFAETKWYQKNK"
     gene            complement(3658..5142)
                     /locus_tag="OUD09_001335"
     CDS             complement(3658..5142)
                     /locus_tag="OUD09_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCT9692612.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQVLIFMGLILGCYWAVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPEGYFLIVEDYDTYAEKQRV"
     gene            complement(5162..5623)
                     /locus_tag="OUD09_001336"
     CDS             complement(5162..5623)
                     /locus_tag="OUD09_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCT9692613.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGEDEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(5641..6708)
                     /gene="ulaG"
                     /locus_tag="OUD09_001337"
     CDS             complement(5641..6708)
                     /gene="ulaG"
                     /locus_tag="OUD09_001337"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HCT9692614.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADSKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            6926..7696
                     /locus_tag="OUD09_001338"
     CDS             6926..7696
                     /locus_tag="OUD09_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCT9692615.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(7969..8797)
                     /locus_tag="OUD09_001339"
                     /pseudo
     CDS             complement(7969..8797)
                     /locus_tag="OUD09_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            9364..10599
                     /locus_tag="OUD09_001340"
     CDS             9364..10599
                     /locus_tag="OUD09_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HCT9692616.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(11598..11858)
                     /locus_tag="OUD09_001341"
     CDS             complement(11598..11858)
                     /locus_tag="OUD09_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCT9692617.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(11885..13186)
                     /locus_tag="OUD09_001342"
     CDS             complement(11885..13186)
                     /locus_tag="OUD09_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCT9692618.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEKAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNATLLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPMIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
BASE COUNT         4194 a         2754 c         2086 g         4198 t
ORIGIN      
        1 ttttataatg tatcagtata gaaaggaaat aaaacatgga ttatactgtt gaatttgaca
       61 gtgaactact taagcaattt ccacaagaag aacatttcat gtataaagaa gcaatttccc
      121 acaaagtgac agagtatttg gagcataatt tttttcgtat aaagccagtg cgtactcgtt
      181 cggcaaaagg aatatacgag atgaaagtca aaattaataa aaaaaattat cgcatagcat
      241 ttgctttaaa aaaaaatcat gtgttcattt tttatattag ttcgaatttg caaaaaattg
      301 tatttgataa agaggtgagt aaaaaaataa aatagctgag cattggagga tagattgagt
      361 gatactaagt ctatcctctt gtttttctaa agaactaagc tagaattttt tcttttgtac
      421 gttattatat gttatttgtc attaccaaaa agcacctttt ttcttctagt atctatgaca
      481 taagaaatac cacccatttc ctttacttga acaaatccta ctgattcata aaattttttt
      541 gttttctcag tatcatctgt caataattcg atttggactg gtccataact agtgatcttc
      601 tccaaagctt cattcaaaag cactttgcct attccttggc gctgatacct cggatgaatg
      661 aggatatctt gaataaatgc gatcgtcgca aaatcagtga tccaacgaac gattccgata
      721 atcttccctt ccccattacg ctttatcaga gattcagatt gttcaaatgc tctagctaac
      781 cttgcagtgt cttttgtgta attcgtccaa ccaacgctct tatataattc agaaatctca
      841 tcgatcataa gggaatcaat attcattttt ttcttccttt cttatacaat tctccctcta
      901 ataatacagt tctttgacta tttaatctat cacatataac tctcctgtaa agattctctc
      961 gattccttaa aatctctctt tccttttaga atgcatcctt tttgataaat aaaaaggttg
     1021 tgataaattt tcttcacaac ctctgtttct ctctatcaag tgttatcttt taattttcta
     1081 cccttaccct agcatctcta ttgcccgtag tatgcatact ttccatgttt gcgaaaataa
     1141 tgtttatcta gtaaatattg cgggatttca catatattgg ggttgatcag ctcgttttct
     1201 ttggccatca gacaaatttc atctaagatc aaactgtttt ctactgcttt cataggtgaa
     1261 tccccccatg tgaacggtcc atgtccatag acaagtactc cagggacttc gtttggatct
     1321 aattttcttt ccttaaacgt ctcgacaatc acattccctg tatgaacttc gtaagcttct
     1381 cttacttctt ctttcgttaa ttggcgtgta caaggaacct taccataaaa agcatcagca
     1441 tgagtcgtgc cataagctgg caaatcgcgt cctgcttgcg cccacatgac ggagtgcgtg
     1501 gaatgagtat gagtaatagc agttatatct tcaaaagttt gatacaaaac aacatgtgtg
     1561 gggagatctg aagatggttt taagctatct tcttctatta tatttcccga caaatctgtt
     1621 accaccattt gatctgcttg catgcattca tatctgacac cgcttggctt aatgacgatg
     1681 actcctagct cacggtccac ttcactcaca ttgccccaag tcaatttgac taatccgtat
     1741 cgcggcaatg ccagatttgc ttcaaggaca cgttgcttca ttgctgcaat tttttcttca
     1801 caagacatca tagccagcct ccttcaattt agaacttaag tagtcttttg cctgctggat
     1861 ttctcttcga aagtcttggc ttttttcact ccacatttca atcagaaatg ttccatcata
     1921 atctagacgt ttcaaatgtc ttaataatcc aagaaaatca acacaaccgc taccaaaagg
     1981 aacatcacga aactggcctg aatattcttt tgttacaggg taagtatctt ttaagtggat
     2041 agcagtgata taatcgatcc ctttctcaag ttctcgagcc ggattatttt ccggccatgc
     2101 ggacaaattg cctaggtctg gatatacttg tagaaaaggg gaatgaatct gttctttgat
     2161 ttctaaaaat tttgaaatgg agttcataaa tggatcatcc atgatttcca cggaaagaat
     2221 gatgccatat ttagaagctt cctttacgct ttcttttatt ccttcgataa agtcttctct
     2281 ggaattcatt gatttctctt cataataaac atcgtatcca gctatttgga ttactttgac
     2341 gcttagatta tgcgccaaat ggattgcttt tttcattagt tgtttcgctg cttttttctt
     2401 ttctggatca gcagaaccaa acggaaatcg ccgatgcgca cttaaacaaa tcgaatggat
     2461 acgtacaccg gagctgaaca tctcttctct cagctcagct atcttttctt cagaccaatc
     2521 taatcgtgct aaccgctcat ccgtctcatc aatcgacatc tcgataaaat cgaagtccat
     2581 ttcttttaca agggaaaaac gttctttcca tgaaatatct ttgggtaatg ctttttcata
     2641 aatcccaatt tgagccattt ccttaccccc agatacgaat gatctcatct ttgaagtttt
     2701 gtgcggcctt catcggatca tttgctgctg taataccacg accagtgata aaggtataca
     2761 catcgatccc ttcaaataag tgtaatgtgt gtggatttaa tccaccagtt acagaaacat
     2821 taaagcctag ttcaatcagt tttttgatct tcgataaatc tttttcaccc caagtttcac
     2881 cagataacag cgcatcacgg ctttgatggt agattacctg ccggataccg atgttatgcc
     2941 aatccatggc ttgttcaaaa gtccaattgc cgtagagttc tacttgcaat tcacctattt
     3001 ctttttgtgc tgcctccata gttggaatcg ttgcacaaca aatgactgtc atccagtctg
     3061 ctcctgcttt tgcaacatta ctcgccactg ttcctccagc gtctgcacat ttcgtatcag
     3121 cgactagttt tttatctggg aacatactgc gaatacagcg aatcgctttt tgtccttcct
     3181 gtaagcaaag aattgtgccc acttcaataa tatctactac atcaccaact ttcataacat
     3241 ctcctaatgc atgttctaag tcattgtgat ctaacgcaat ttgcaaattc ggcctgctca
     3301 tcttagttcc ttcctttact tgtttttttg gtaccacttt gtttcagcaa atttttcact
     3361 gatttctttg gctgacatca catttttgat accgataact gtaacttctt ttttctctgc
     3421 ctgagtaaac atattcaaaa aattgactgg cgtaaaaact acatcgtatt ggccagctga
     3481 actttttccc tctgaaatgg cacaatgatg gattttggta attggtacgt ttagttcttt
     3541 cattgctttt tccacacttc tcatcatcat caagcttgtt cctgatccat ttgcacacga
     3601 tactaaaatt ctcatcttaa tccctccatt atgagtgttg tctaatcaga ttttctttta
     3661 tactctctgc ttttctgcat atgtgtcata gtcttctaca atcaaaaaat aaccctcagg
     3721 atttttccga tattgtaact gcggaatagc caataaacca atgacaacta cggcaattcc
     3781 agcataacct aggactttca tgattactgt aaacaatggc caagctgttg cccagtcgaa
     3841 catcccgata taaccaccat atgttgacat cccaatccat gtagcgaata atgctgaacc
     3901 gcctacttgt atgattcctg aaagaaacgg gaacaggcaa gcggctttga atccacctcg
     3961 gttattggca taaactgcta taacggcatt atcaaagaac aacggaatga aaccggctat
     4021 gacgatcgtt ggtgatttca ataaaatcag taaaccaatc atcaaaaact gtcccaaagc
     4081 accaaacaag aacccaatcg ttaccgcatt tggcgaacca aagccaaaag tagcagctac
     4141 gtcaattcct ggaacagctc ctggtaaaat tgtgtttgag attccttgga aagattgagt
     4201 tagttcatcg acgaatgtcc gaacacctaa ttgcaagatc gccagatata ccgcaaaatt
     4261 taaagaagtc gtcataatgt agaataagaa actctgtcct tcttgcatga attgcgcttg
     4321 aatcaaataa tctttgccta gtacaatcag aatgatccca aagaaaaata gcatgagtaa
     4381 cgaagtagca accatattct cattgaaaat cgacaagaat cctggtaatt tgatatcttc
     4441 gatttttttg tcttgcttga ttgatctatt tttcttttca tcccgctttt tcatccactc
     4501 agctaatttt gcaaatataa agattccgaa catttgctga tgtgccactg caaatccggc
     4561 accttctgtc aattcctgcg tgattcctac tgttaagtta gaagctactg cccaataaca
     4621 acctaaaatc agtcccataa aaatcaatac ttgtatccta cctagatcag gaaagcaaaa
     4681 cagtaaaatc caaaaagcgg tagctgcttg ttgaacctga acgtttccgg tagtgaaaac
     4741 ggaacgcagt ttcgtatatt tctgaaaacg gactaataag atatttaaaa caaatgcaat
     4801 cagtaataaa atcatcacat ctccaaaggt acggccaaat gtctccatta atccagcatc
     4861 cacagcattt tgtccaaaat aaggatctgt aaccatggca tccaaattga accgttcctt
     4921 tagtccaacc aaaatcggcc gaaaattatt gaccaaacca ccagagccaa cagtcaaaat
     4981 aaagtaaccg accgttgcct ttaagaatcc agcgatgcat tcatgaagcg ggcgtttcag
     5041 caaaatatag cctaataaca cgataaaacc aatcaggtat gctggctgcg tcaagatatt
     5101 tgttgcaaaa tacgtccaaa tgcttaacaa aaattccccc atcttttttc tcctctatca
     5161 attaattata ttctgccatc acaacttggt aatcatcgat ggagttcgta gagatgagac
     5221 gctcaaccaa tccatctgtc attaataatt cggataattt ttggatattt tctagatgtt
     5281 cttctggatc ttttgctgca agtgtaaaaa acaaactagc cgttttctcc tcatcctccc
     5341 cttcactttc aaaacggatt ggctgtttca acttggtaaa tgaaatagct gttccaaaaa
     5401 caccatcact tttttctgaa gaatgcggca ttgctacttg aggtgcgatc acaatataag
     5461 gaccgtattt ttctacacaa tggatgatct cctccacata cgtttcatca ataatttcct
     5521 tatcgatcaa tacaagacag ctttcggcga tagcttcacg ccaatccgtg atttctttat
     5581 ctgggtaatg aatcaattga tggtcaaaaa agtatttcag caagaaactc cccttctttc
     5641 ttataaaaac gacggaaacg gtgtatcatt ttcaatcgag aagacatctt caaatcctcg
     5701 ataaaaatta aactcaagat catctttgtt tgtcggatag atgaatttcc cgcctacttg
     5761 ccagatataa ggtttaaatc catacttcag gcgatccttt ttgaatttcc aaatttcagt
     5821 gatctctttt gaatcagcca taaaattaga ccaaatatcg tagtggacag gaatcactac
     5881 ttttgcattc aatgactccg ccatgcgcag catatcaaca gaagtaactt tgtctgttat
     5941 ccctcgtggg ttttcaccat aggcccctaa acagacatcg attttgtgtt cattaccatg
     6001 tttggcaaat aaattggagt aatgtgaatc tcctgcatga taaagattcc ctcctgatgt
     6061 ttcgaataaa tagtttactg caatttgatc catctcctga ggcatttttc cttttaatac
     6121 ctcttctgga tcttcacaag tgaccaacgc tgtccgatca aacgcttcta aagctactac
     6181 attgatgtct ttgattttga ctgcatcacc tggctttact gtaatcgttt tttcttcagg
     6241 aattccccat ttttgccacg tgcgaaccac ttcttttggc ccaatgaatg ttgcttcagg
     6301 acaattttga tgaactgctg cggctgtatg gatatctaga tgatcggaat ggatatgtgt
     6361 aacgattaaa gcgtctacat tttttatcgc aaaaggatcg attacgaaag gctgagtacg
     6421 taaattaggc tgcatatttt gaacgccact catacgcatc atctgatggc cttttttcat
     6481 catgccattc ccatgactac gttttcccgt cccacaccat agatcacata aaatattcgt
     6541 tccttcatga cttttcagcc aaattcccgt acagcccagc caccacatag caaaagttcc
     6601 aggatccact acttctgttt cgatttcttc attcaagtat gtgccccact caggaaatgt
     6661 ttctactaac cacttttctt ttgttacttc attgacagtt ttactcattt gcttgctccc
     6721 cccatatcgt tgttccaact tacaagtaaa tattaatgtg tatcagcatc catggaaacc
     6781 cttttatatg accgtttgtt ttttatatga ttttctttca gtaatcaatg attgtttcag
     6841 tataataaag acagatatga atgtttaaat gaacaactag tcaaataaat ccatggatca
     6901 agatgtccct caggaggcaa aagtcatgaa aaactcactt gtcaatattg aaaaacgtca
     6961 aaaagaaatt ttggaactgc ttcagcagac tgaacaacta tcaactttcg aattagctga
     7021 atcccttgat gtatctatta gtacgatacg aagagattta aatttattag aagaaaaaaa
     7081 cgagatcata cgcaattacg gttattgttc ttataattac aaaaaccaga ctgattttga
     7141 tcaatctggt cctgaaagga ttaaacaagc gattgccaga gaagctagta aatacattaa
     7201 cgattatgat acgctgttta tcaattctag ctcgactgcg ttgaaaacac tggattttct
     7261 tcaatctaac catttgacag tcattacaaa caacgtgaaa atcaattact ctccgcacaa
     7321 agataattgt agttatattt taactggtgg agagatgcgc tttccaaaag aagcactagt
     7381 gggcgacatc gcattaaata cgatttcttc agtcaatgca gacatttgta tcattggctg
     7441 tagcggagtc gatgtagtac atggggtcac aacaaagata ttaaatgaat caacgatcaa
     7501 cgaaatgatg gtcaacaaga caatcaaatg taagatttta gtcgctgatc atcgaaaaat
     7561 cggactgaca tcaaagttca agattgctga tatttcagtc tttgattatt tgatcactga
     7621 taaattttgt tctgaaaaag tattagagga gatcagaaat acggggataa aaaccattca
     7681 agtagattca ttttaagaac caggacgtaa atatgtaaat aggattatca aaaaaatgaa
     7741 taacaaattt atcgataaat gaaaaacacg ctacgctcaa attgataaag gaatcccaag
     7801 atgtgtccat ccaatcgata ccatcataat ctattgcttc ttttataacc gttatatgtt
     7861 gcctaaatag gaatcaaaca atatcgaaaa aaggaggtat gacaaaagtc acacctcctt
     7921 ttttcgaata aacggtattc aaatcagaac actgatttca caaccacttt atttcaggtc
     7981 gttttcaatc acctttaccg cgtaatcatt tccgttgaaa cgcgctattt tttttgcgtc
     8041 catatatctt tctaaataat catcttctcc taattgctta tggcaagaag caatttcata
     8101 aagcaagtat tccaatgcat aagtcgattt atgtttctta ttatactcaa tacccttctc
     8161 acataattgg attgctttat cataatcctc gattttacgg taataggtgg cgatatcata
     8221 atatacaatg cagaccatca gattatcaaa atcagtctca ctcaatattt taagtgtacg
     8281 ctccacataa actttcgcaa aatctaattc tccttttgac aagtagatca ttgccacgtt
     8341 tgcagctgct tgtaagtgat ggatatctgt ttcggtcgtt tgttccaaca cttgattaaa
     8401 ataaaataat gctgcatctg gtttttttcg cataaagatg atcaagcagt ccttgataaa
     8461 ataaaatttg atttaaaaga tcagaattac ttactttttc ctgatcgatc gtttgtaaaa
     8521 ttttataaga ttcttggtat tttgatacag aattcaatga ttgaatctca tttatttttt
     8581 tcctgatttt gtcttcttct gtttctacta atatatcgga gatctcgaca ttcagctttt
     8641 tgcaaagcgc acttaagata ttgatgcctg gcgacatatt cttgttttcg atattgctga
     8701 cagttgcttg ttcacaaatg ccttctgcta attgtacttg tgttaacccc tttttctttc
     8761 ggtattcttt tagtaatgct ccatctactt taatcaaaac aatttccttc cctttaaaaa
     8821 attatttctc ctttatatca tacacaaaaa aaagttctaa acaaccaaaa atgtttagaa
     8881 cagaataaaa attttaattt taaaataata ttcaaattaa acaactggcg tatctgctcc
     8941 ttgatttctt tttgtgttat ctgaataatt atcgtttaca gttataacac atagtgaaaa
     9001 ttgtcatggc gagaaagtct aagaatattt ttaaacataa ctatttcttt cttacgtgtg
     9061 tataatgacg acagcacacc tatcaattaa tataattatt ttatttttta tgttctcatg
     9121 tttattttta atgacacaac ttctcgtatt tatctggcac aacatataaa tcgatcaaaa
     9181 aattgttgcg tgtttttttg aaaattggag tttcaacttg attacaaaat ttaaatttag
     9241 aagttgtgaa aacagcgaga gacagtttaa tattcaaaat atacataggc tactcgaacg
     9301 ctgtgttttt tagtatacta gtaagaaacg aagggataga ctcattccaa taaggaggat
     9361 ttcatgaaga caattctttt tgctcctgca gtattcaatt tagctgagac aacacgaatg
     9421 attgaggttg ccaaagctat gaaagaccgt tattactgtg tattctttgg cttttctaaa
     9481 acttttgctc ctttgatcac agaaagtggc tttgagtatc atttactagc gccgatcctt
     9541 acgaaaaaac aagaagaaca aatcataaaa tttgaccagt tgcgtacact taaaaaccct
     9601 tttaccaaag aaatggttgc ctctcgtatc actagtgaac agagtctgat tgctaagttt
     9661 gagcctcgac tgattgtgac aggttcgaat gtgacaattt ttttatcggc ccgcaataaa
     9721 aagattcctt tagtatatgt taaaccctat gctttgagtg aacctttttt tcaatcagag
     9781 catgcgctga tgccccacga gttacgtttc ttcggccctt taagcaatcc cctttggcac
     9841 tggacaaaaa agcgtatagt aaatacccga tggatcccca atgccttcaa acacgctgca
     9901 caaaaagaaa tgcttccttt accaaaaaca agtttacaaa tgatggatgc tgatctaaat
     9961 ctcatcacaa cgcctaaact atttttggaa gaagaacagc tgccggaaaa ttatcgaatc
    10021 gttggtccaa tttttgctaa gctcagtact tccttgcctg aagaagtgct aacttttata
    10081 cagcgaaagc aacaagaaag aaaacgtatt atctatttcg caatgggtag ttcaggcaat
    10141 ccgcgtatgt tacgcaaaat tttgttcttc ttacaaaaac aaccggatct agcggttatt
    10201 gcacctattg actatctgct tgatgacatt caatctgact cagaagatct tttaataaca
    10261 gaatatctgt ctagtcatct tttgcatgaa ttcattgatt tttcttttat ccacggcggg
    10321 gaagggaccg tccaaactgc ctgtgaatcc ggcaaaccgt ttatcggtgt ggggatgcaa
    10381 tttgagcaaa gctgtaatat tgactattgt gtaaaatacg gaaacgcaat cgccctcaca
    10441 aaaccagtga ctgaaagaaa actgcagatt gctctgcgcg aagtttcttc acccaagatt
    10501 cgtcagcagg cacgcacgct gcaagccgtt tttccaataa atggtccaca acaagcaatt
    10561 caagaaatcg aaaccttcct gagcaaccac ccaatatagt ttcaagtaga aaaagctttt
    10621 ttaatagaga ctcatcagta atttcgctat tcgagaccca gacagtgaaa atacaaaaaa
    10681 agtaaatagt ggattgaaaa gttagagtta cttaaattac ggtgtctaaa aaacacaaaa
    10741 tcatccttaa aataaaagat ctgacgcaat ccaaatgagc ataggtagcg tctgccattt
    10801 attcttagtc aaattctttg acacctttct ctggttggat catctcaacg tttcttcaaa
    10861 agaaagacca taataattag gcaaatacat tttcttaaaa tgtgtcatat agatctagaa
    10921 gcatcgcttt gtagagattt gtgctgctag tcccttcaac aattactaaa tcaaatgtag
    10981 gatttaccac tttatctttg ataatcaacc tccctcttct gttgagacaa tgatctcgca
    11041 atccgatgca tactcctata taaactgcca gcaatcccca catgctgagt agatcatgtc
    11101 agaatgttct atgtcgtcag caacgataaa ctaaaagtca cgttcaccat gactgattgc
    11161 tattcccagc gcaattattt ctatacacgt ttcatgattt gaataaacac tctcatcata
    11221 catattacca ttcatacacc atactgcagc ccctactata tggttgtaag ctcccaaatc
    11281 tatatttgcc ttgatcactt tggtagtaga tcgactaatt cttaatccat tgctgataaa
    11341 attttcattt ttgcctcctt ctttctgcca aaacagttaa aacaataata atctttatgt
    11401 aaaaaagggc tgtgacaaaa gccttgtcac agtcccatat tccgaataaa cggtggtcaa
    11461 aagcttgcat ctgcggtaat aagttcaacc aaacgaaaaa taagccaaca tttcacaaaa
    11521 atttaagaag caattttcgt aaatttcttc ttatttcttg aagctaacca cttctgtcac
    11581 aaccactttt agaacattta aagcttcgat gtgacaaacc atctatgctg ctcgatcata
    11641 aaattgatag caaaaattcc acaaagtaca tatgtccccc atgaaaacaa aacattcata
    11701 tgcggtacaa tgatctttgg aagaatcacg atcaaaaatg atgccagtgc ccaaatccat
    11761 cgattgattt gaatatgata tgtagctagc atattcaata taaggtgact agccgttaat
    11821 aaaaagataa aaacaaaaaa taaaatcagc caatacattg tttcccacct tctaaaaacc
    11881 aaacttagac agtttggact tcgttttctc cacgactttg ttcatctgac agcatctttt
    11941 tatcataagc ttttacaaat ggataataga taataaatgc tatcaatgtg ttgatcatga
    12001 tcaagataac aacacgccag tcatttcctg ctgaaagata accgccgatt ggagctggta
    12061 atgtccaagt tggcaaaaca gtaaatctat tgaccatttc taatttgact gcaaaataac
    12121 tgataatagt caaaactaaa ggaactaagt tgaacgggat gaacatgtaa ggattcaaca
    12181 tcataggagc tccaaaaatc atgggctcat tgatattgaa gataccagga ataatagaaa
    12241 aacgaccaac ctctttcaag aattttgatt tacagaagaa tataaacaaa acagatacca
    12301 cgatcgttgc tcctgcacca ccaattgtta ccgtccattg ataaaactgt tcaggagcga
    12361 tatagggtaa ttgggtggca gctgtaccat ctgccaatgc ctttgcattc tcatccaaca
    12421 tgatcagcca cattggacga acaacagccc ctacaatact catgccatgg ataccaaatg
    12481 accacaacaa atggatcaaa aacattggta gtaaaacacc aagcaaatta ttccctaaaa
    12541 tattggttaa aggctcgaat aacgataata aggtagcgtt tacatcaaag cccgcgacgc
    12601 ttcgaatcag ccaagtaatt gttaaaatga ctgcacctgg aatcaatgcc tcaaaactac
    12661 gggcaacagc aggtggcact tgttctggca ttttgatagt aatatttcga tttttaaaga
    12721 aacgatatac ttccactgca aaaatcatag ataagatccc cgcaaacatc ccagatgctc
    12781 ccaaagaact cataggtaag acccagccaa gaggtgcctt ttcaggagtt aaaacgttta
    12841 caggaacttg taacataaag aacgttccta aagataatat accacctgtc acactatcta
    12901 atccatatga tttagctaga tccgaaccca taacaaaagt tgcataaata gccatcaatc
    12961 ccatcgtcat cctaaaaggt aaaacaatcg ttgccgaata aggggcaata aattcattcc
    13021 atccaggaaa cggtaaattg ataaagacca taaaaaatgt cccaatcaaa atcaatgaca
    13081 atgttgaaac aaccccatta cgaattgcta ataaatgacg ctgtgtacca atttttgcca
    13141 ttggtgggac aagtttctct tcgacccatt tcatcagctt ttccactaaa accactcctt
    13201 ttcaataaaa tatgaaccat tcattgcatg ac
//
LOCUS       DAKBVG010000068        13203 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000068 DAKBVG010000000
VERSION     DAKBVG010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.068"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(134..206)
                     /locus_tag="OUD09_001343"
     tRNA            complement(134..206)
                     /locus_tag="OUD09_001343"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(171..173),aa:Lys,seq:ctt)
     gene            587..1156
                     /locus_tag="OUD09_001344"
     CDS             587..1156
                     /locus_tag="OUD09_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HCT9692619.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1143..2003
                     /locus_tag="OUD09_001345"
     CDS             1143..2003
                     /locus_tag="OUD09_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HCT9692620.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2193..2897
                     /locus_tag="OUD09_001346"
     CDS             2193..2897
                     /locus_tag="OUD09_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCT9692621.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2902..4737
                     /gene="walK"
                     /locus_tag="OUD09_001347"
     CDS             2902..4737
                     /gene="walK"
                     /locus_tag="OUD09_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HCT9692622.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4734..6050
                     /locus_tag="OUD09_001348"
     CDS             4734..6050
                     /locus_tag="OUD09_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9692623.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            6051..6920
                     /locus_tag="OUD09_001349"
     CDS             6051..6920
                     /locus_tag="OUD09_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HCT9692624.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6975..7784
                     /locus_tag="OUD09_001350"
     CDS             6975..7784
                     /locus_tag="OUD09_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCT9692625.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7887..8516)
                     /locus_tag="OUD09_001351"
     CDS             complement(7887..8516)
                     /locus_tag="OUD09_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCT9692626.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8686..9978
                     /locus_tag="OUD09_001352"
     CDS             8686..9978
                     /locus_tag="OUD09_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCT9692627.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(10054..10716)
                     /locus_tag="OUD09_001353"
     CDS             complement(10054..10716)
                     /locus_tag="OUD09_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCT9692628.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10941..11225
                     /gene="groES"
                     /locus_tag="OUD09_001354"
     CDS             10941..11225
                     /gene="groES"
                     /locus_tag="OUD09_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCT9692629.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11276..12901
                     /gene="groL"
                     /locus_tag="OUD09_001355"
     CDS             11276..12901
                     /gene="groL"
                     /locus_tag="OUD09_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCT9692630.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4388 a         2273 c         2843 g         3699 t
ORIGIN      
        1 gagtgtaaca taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgaaaataa
       61 aaaaagaaca gcgtaaatgt tttcgaaatg ctttcgcacc aacatttacc tgttccgtgg
      121 gtttggcacc caatgggccg tgaggggctc gaacccgcga cccgctgatt aagagtcagc
      181 tgctctacca actgagctaa cggcccgtac aaaaagtact taataatagt agcactaaga
      241 attttaaaat gcaagactac atttaaagaa aatcagaaaa atctttactt ccttatagga
      301 aatcattcat agattattca catttgctcg ctattatata agcataccaa caaacaaagt
      361 tcttgcaaga gaacaaaccc taacactttt ttcatttttt actcctttcc cgccgtctcc
      421 ccggcgggtc tcctgttctt aacgataaaa gacaagaagg cttagccttc ttgttttttg
      481 ttttcatgga atcccgctaa tcaaatcacc tagttaaagc aactagattg aatgtttatc
      541 ataagtctga tatcatgttt atgaaatgaa aaaaggagcg gttatgatgg aaaaaatgcg
      601 tcagcaatta atggagtggg ggaaggaact agaaacattc cgtctgcctc actgggaaga
      661 actccctgac ttagaacttt atatggacca agtcagaacg ttagttgatc gttatctttc
      721 acctgtgata caaggagaaa agcatccatt gcttacctct tctatggtaa acaattacgt
      781 taaactaggt ttgataccag caccagttaa aaaaagatat aacaaagaac acgtagcatt
      841 tttacttgca atcactacgt tgaaacaagt attgactatt cctgaaatca aagaagggat
      901 attgttccaa ggaaaaacag taggaattcg tgaagcctat aatctatttt gcgatgaaca
      961 agaagcagct gtgtggatgg tcagccaatt agcacaaggt aaatctcatc cgcaaaaatt
     1021 cgaccaggca acccctgtag aatatattgc tgtaagagcg gcaactttat cgtttgcaat
     1081 gaaacttctt gcggaaaaaa cgattgttct agaaaccgaa tatttaaaag aggagaaaac
     1141 aaatgaaaaa caataaaatc gcaattttag tagattcagg tacggatgtg ccagaagaat
     1201 tgataaaaaa atacgagatg tatatgatcc cattgaaaat tatttataaa gatcaggtat
     1261 atacagataa aattgacatt acctctgaag agatctatca acgtttgcca gaggaaatcc
     1321 ctggtacatc tttgccggat ggtgagtcga tcacagaaat ctttgagcaa atcaaaaaag
     1381 acggttatga aaaggtactt gcggtaacaa tttccagtgg attgagcggg acatataatg
     1441 tggttcgttt aattgcacaa cagcaagaag aattggaagc atatgtacta gatacgaaaa
     1501 atattggtat tggtgcaggt ttttctgcga tccaagcagc aaaatggctg gaagaaggag
     1561 tagaatggga tcaactgatc agtaatctga atgagttagt cgaaagaaca aaagttttct
     1621 ttaatgttgc gacattggaa tatcttcaaa aaggtggacg gatcggttta gttgcttcta
     1681 ttgtaggaac cgctttaaag cttaacccaa tcatctcatg taatgaagaa gggatttatt
     1741 acacagtagg aaaagctagg ggacgtaaaa aaagtttgga taaaacggtt gcttacgtca
     1801 aagaaaaaat cggtaatgca aaggtcttca acttagctgt tgcccatggg gatgcgaaag
     1861 aagaagcaat tgaaatggaa gcgagactta aaagagagtt cccacaagca aaccaaatct
     1921 actttggcca gatttctccg gcattagtag tccacactgg tccgggatta ttgggtgtcg
     1981 gtgtacaagt tttagaagga taaatcaaaa aaaggaaaca ctgaggtgaa aatcagtgtt
     2041 ttcctttttt attgaagtta gaacaaaaaa taataaagta ttttgcactt tcattttaat
     2101 tttctctttt gttttatagg caaagatgaa acaaaagcag aaatacggta taatgagaag
     2161 gtatgacaat aatatataag aggtggctct ttatgaaaaa agttttagtt gtcgatgatg
     2221 agaagccgat ttcggatatc gtcaaattca atttagccaa agaaggctat gatgtttata
     2281 ctgcatatga tggggaagaa gccttggaaa aagttgctga ggtagaacct gatttgattt
     2341 tacttgattt gatgctacct aaaatggacg gcttagaagt tgctcgtgag gtacgcaaaa
     2401 cttatgatat gccgatcatt atggtaacag ccaaagattc tgaaattgat aaagtcctag
     2461 gcttagagtt aggcgccgat gattacgtga ccaagccatt ttccaaccga gaattagttg
     2521 cacgtgtcaa agcaaatctt cgtcgcggtg ccacggctgc gaaggaacca gaagaggcag
     2581 cacctgcgga attgacgatt ggtgatttga cgatccatcc agaagcttac atggtgacga
     2641 aacgaggaga aacaatcgag ttaacacatc gtgaattcga actactcttt tacttggcga
     2701 agcaccttgg acaagtgatg acaagagaac atttattaca aacggtttgg ggttatgatt
     2761 attttggaga tgtacgaaca gtggatgtaa ctgtacgtcg tttgcgtgaa aagatcgaag
     2821 ataatccaag ccatccaaat tatttagtga cacgtcgtgg tgtcggttac tatttgcgta
     2881 acccagaaca ggagtaattg gatgaaaaga aaagtccgct tttttcgatc agtcaatttt
     2941 aagatcgcta ttacctttat tctgatttta ttgatctcga tcgaaattat tggtgcctac
     3001 tttatacgtg gtttagaacg ttccacgatc aatacattta ttaaagatat gaatcaaacg
     3061 gtggaatcat tagcaacaac aatcagcccg gaattgaatc gaaaagataa tgcagatgat
     3121 gaagaggtaa atgcgaatat caaaagattt attgaaaaca gtgcgactag cgatatcatc
     3181 gaaatacggg tcgttgacga aaaagggatc atccgaggaa caaccgatgt caatgaacaa
     3241 agcgctgtgg gaaagaaaaa tgattatgtt gatatcaatg atttcacgac taaacgttat
     3301 gttgctttag ataacgacga taaacgggtg aatatcaatg ttcaaccgat cctgtccccg
     3361 accggcgatg ccgttatcgg ggctctttac gtcaaaagta atatcgaaca aaaatattcg
     3421 gaaatcaata acacagcagt cattttcttt actgcctcac taattgcggc atttatttca
     3481 atgatcgtat ctgttttagt tgctcgttcg attacacagc cgatcggtga aatgagagaa
     3541 caagccattc gaatcgctag aggagactac agtcggaaag tcaaagtgca tggacaagat
     3601 gaacttggac agctggctga taccttcaat cagttagctg aacgtattga agaagctcaa
     3661 gatacaatgg aagctgaacg caatcgtttg gatagcgtgc tatcccatat gacagacggc
     3721 gtcattgcga cagatcgcag gggtaaagtc attacgatca atgatatggc tgtttccttg
     3781 ctagatatca aaaacgagga agcaatcgga caatcgattt tgacactttt agatatcgaa
     3841 gaagaatata cgttgcggaa attattagaa gatccaaacg aaatggtact tgaccgttca
     3901 accagtacat tggaaagtga tcagatgatc ttgcgagtag acttttctat gatccgtcgc
     3961 gaatcaggtt ttatcagcgg attagtagct gtcttgcatg atgtgacaga acaagaaaaa
     4021 acagaacggg aacgtcgtga attcgtctct aatgtttccc atgaacttcg tacaccgttg
     4081 acaagtatgc gtagttatat cgaagcattg agcgaaggtg cgtggaaaga cgaggaagta
     4141 gcaccaaatt ttttgaaggt aacgctagat gaaacagacc gaatgattcg aatgatcaat
     4201 gacctcttaa atctatctcg catggataca ggtaataccc agcttcaatt agaatatgtc
     4261 aatttcaacg agttagtgaa ttttgttctt gaccggtttg atatgatggt gggaaatcag
     4321 gaaaaaaatt ataagatccg tcgtgaattt acgcaaagag atctttgggt cgaagtagat
     4381 acagataaaa tcatccaagt agtcgataat atcatgaata acgcgatcaa gtattcacca
     4441 gatgggggaa cgattacttg tcgcttatta gaaacgcata ataacgtggt tttaagtatc
     4501 accgatcaag gattggggat tcctaaaaaa gatctgaacc gagtgttcga gcggttctat
     4561 cgagtagata aagcacgtgc aagagcacaa ggtgggacag gacttggatt agctatttct
     4621 agagaagtga ttaaggcgca tcgaggcgct atttgggcag agagcaagga aggaaaaggg
     4681 tcgacttttt atatctcgct gccatatgaa ccttatgagg aggaatggtg ggaatgagaa
     4741 tctctgaaaa agtcgttcgt ataggactca ttttgatggt tgcgttaagc atatacttct
     4801 cttatgcaat ctggctgagc ccagcaggaa aaagttcaat caacgtagat gagacaaaca
     4861 ctcaaatgat cgagtctcaa agcaatcgaa aaacttccga agtctttctt ccgcttcatg
     4921 ttacatggca ccattcggga atgatccaag aaaccaacag tgaaaatctc gtgtaccgat
     4981 tgcaattgat catcgataaa gcaagatttg gaaagctttc tgaagtagtt tcaggagaca
     5041 aatcacagtt tgaacaggtg aaaaatctga actctggttt tgagttggcc tataatgcgc
     5101 cttttttgct aagagaatac aaggaagcat ttgatttaga tttagatgtg tcgaacttgg
     5161 caaaagaaag cgaaaatatg tattttacta gaatacaatt cgatcaagga aataaaaaaa
     5221 tccgattttt gaattattcg aaccatctcg tatatgaagc aaatatttcg ttgaattgga
     5281 cagatatcaa taaagaatta acagcaaaag acacaaaatg gacagaaatg acaacagaac
     5341 catcgacgat cgacacgcaa tatgctacga aacattcgat caaactgaaa aagtatagtt
     5401 atatcctgtc acagcaaccc tatacacttt ttagaaatgc ttttttccag aaaccagata
     5461 gcgtgaaaaa taatgatgaa agcaatgaat tgatttttta cggtgggaat gaaacaatgt
     5521 ctatccattc ggaaacgcaa gtagttgatt ttcgtggaga gctgccagag aaaaaagaaa
     5581 atgctagtgt attttctgaa agtttcccct atattagcaa attaggcagt tctttaggga
     5641 acctcagata ttttgaccgc agtgacaata cgatcgatta tcggatattc gtggaaggct
     5701 tcccggtttt tgggacagac agcagaggac agattggtgt ggaagttggc ggtgaaacga
     5761 atggttctgt gcaagtgaat atccaaacca gtttgaatac gattcaagtg ccgatacctt
     5821 cagacgaaga agtagaactt gcaagtacga atgaaatcat ccagcagtta aaagctaagg
     5881 gagcagatag caacaagatt ggttcagtga tcgttggcta tacatggcaa aatgtcaaag
     5941 aaacgaatcg tgtcgttgat ctcttacctg aatggtatgt gaaatataaa gataactggt
     6001 attcagctaa cgaccttctg gaaaaattac ctggattgga ggcgaattaa ttggatttca
     6061 aaaaaatcga atggatattt ttcgtcgctt ttttagggtt gaatgtgttt ttattcagca
     6121 gttatcatga cgcggtatac caagaaaaca atgtcattcg ctctaatcag actgaatcaa
     6181 tccaacaacg tctggctagc gacgatatca cttacacggg gaagatttcg tctgagaaca
     6241 agcaaggcta ttatcttagc gcggagcaga ccaatcttag cacttcatta gcgaattatc
     6301 gcaaggctgg taaaagcggc ttgctggcaa gtggtgtgtt tttagacggt gatgttttga
     6361 cgaaatcttt atccagttca gatgctgaaa agaacgtgat cgatccagat aaaattagta
     6421 gttccttgga agatttttta tctaacaaag acaatatttt attcggtaat gattatgtgt
     6481 acacgaaaaa cttttctcat ttagaagaag aaccactaga aatccaagca agccaagagt
     6541 atgaaggcat cccattcaat gatgatacag caaaaatcgt tctttcttta gaaaaaaacg
     6601 ataatgatta tagcatcacg aaatacatgc agacgcattt atctgatatc gagcagctgc
     6661 gcgaaaagac ggaactgtat acggaagagg aagcaatcaa tacattgtac atcaataaca
     6721 aaataccaag aggatcaaaa attttatggc gtcagctagc ttattcctgt attctgaaag
     6781 tccgggaaaa aaatgtgtat gttcctgttt ggcatgtagc gatcgaaaca agcgataaag
     6841 tggtccaaat tgagagtgtc aacgctttca gcaatacgat cgtgacaaac aatactgtac
     6901 caaaagtgga agatcaataa aaaagcgagt ataatgaaaa aagtttacaa agtaagaagg
     6961 gaagtaggag tcaaatggct gaagaaaaac cagcttttaa aatcagtatc ctagcaagtg
     7021 gaagttcggg taattcactt tatatcgaaa gtgaaaaaaa acggcttttg gtcgatgcag
     7081 gacttagcgg taaaaaaatc acttccttga tggctcaaat tgatcggaca ccggaccaat
     7141 tagatggaat acttgtgacg catgaacatc gagatcatat acatggcgtg ggtgtcttgg
     7201 ctcgtaagta tcatttggat gtttttgcca atcagcagac ttgggaagcg atggaaccac
     7261 tgattggaaa tgtaccagtg gaacaaaaac atctatttga gatggggaaa gtcagaacat
     7321 tcggcgatat cgatattgag agtttcggcg tctcccatga cgcagcggct ccgcaatttt
     7381 atcgtttcca taaagaaaat cggtcattcg tcatgttgac ggacaccgga tattgcagtg
     7441 attatatgcg aggtgtcatt gaaaatgccg atggttattt gatggaaagt aatcatgatc
     7501 ttgagatgtt acgcatggga ccttatccgt ggaatttaaa acagcgtatt ttaggagata
     7561 gaggacatct ttccaacgaa gacggtgcac ttgtcatgac tgatgtgatc ggtaatcgga
     7621 ccaaacgaat ctatcttggc catcttagca aggaaaacaa tatgaaagaa cttgctcatt
     7681 taacaatgga aaatgttttg aaagaaaaag attttggcgt tggacatgat tttgaaatct
     7741 atgatacaga tccagatgaa gcaactgagc tattttcgat ttaaacagtt caatcaataa
     7801 aaatataaaa agcagaagat aaaatcaatc agggttggtt ttatcttcta tttttatttt
     7861 tttagcatat taaaaaaata accgtattat cgactgacta cactgatgtg ctgcttttca
     7921 tgtttctcat ccagtatctc atctactaat gcaatcgcgt aatctgccat gctgatcgta
     7981 ctgtcgccag cagcgtttct ttgtaaataa tcatcactta acaaataagt acctgtacgt
     8041 ttgccatgtg gatcaaataa agaagctgga ctgatataag tccaatgtag atttttagat
     8101 tgtttcaatt ttaacaatcc ttcgtacata ttgaaagctg ttggatagaa gggtgcattt
     8161 ttaggtactt ggtcaatcat acgagtcgtc ttttctgtat ctaagaataa cgaagaggca
     8221 cccccaacga caattaagcg tgtatcggtt ccttcaagca tctcaatcaa atgggacaaa
     8281 gaagtttgat gcatttcttc ttttcctttg ggagcgttga aagcatcaac gacaacatca
     8341 aaagatgcaa gatcagtagt tgtcaactca aataaatcct tttgtataaa cggaacatct
     8401 gatggcaatt tttctggatg acgcacgatt gccgtcacgt ctaaatctct ggacaatgct
     8461 tcgtctaaaa taaatgaacc tgcatggcca gtagctccaa tgagtgcaat tttcataaaa
     8521 aacctccttt tttattcctg tccattgtaa gcagttgctg aatattcgtc aaagaatccg
     8581 atttttgaaa agctcagtgc gcattccttt catggttcgt gatcattggg tatagttaaa
     8641 ttaagaaaac tgattgataa aaatagaagt tgaaggagat aagaaatgaa agagtggtta
     8701 gatcgtgcct ttttaccaat gataacatct gttgtgaatc ttagaccctt aactgctttg
     8761 cgtaatggaa tgacttacac gatcccaata attattgttg gctcgctgtt tttcttattt
     8821 gctcattttc ctgtggatta catcgctcag tggatagaca aaacaggatt ttctatgtat
     8881 tttacacaag tataccaggc tacatttgat gtgatagcct tttggatcgt ttttgccatt
     8941 gcatacgctt atgcaaaaga agaagacatc gatcgcttac cagtcggcat aacagccttg
     9001 tctgcttatc tactgcttat tcagccacta aatactcaga tagatactgt ctggacaggt
     9061 gctgaaggga tactaggagc agttacaact gggctcatca tcggatgggg ctatgcacgt
     9121 ttagtaagac ttgcgaaagg gtggaagcaa agagagaaga tacctgaaaa tgtttggcag
     9181 tcttttactt cattgattcc aatcattgtt atagtcactt ttacgcttat cggttcagcg
     9241 gtttttcaac agttgttcag aatgactgtt gttcaaggag tatggacata tgtccagctt
     9301 cccattcaat cgttcataga cacgttgttt ggtgtgcttt tattaggttt ttttgttccg
     9361 tttttatggt tatttggtct tcatggatct gcgatggtca atgggttggt tagtcccgtc
     9421 ttgcaagcga attcattagc gaatgcagaa atcttagcct caggacaaga gttgacggtg
     9481 gcaaatggcg gacatatcgt tactcagcaa tttctcgatc agttcatgac ggttacgggt
     9541 gctggattga cgttaggggc tgtatttttt atgacggttt ttgcgaaatc gaagaaatac
     9601 agagaattag gaaatcttgc tcttttacca gcctttttca acatcaatga atcaattatt
     9661 ttttccacac cgatcgtgat gaacccgatg atggctgttc cttttatttt cgcaccggtc
     9721 gtttcaggat tgattaccta ttccgcttta tattttggat tcgttccttt atttacagca
     9781 gtccaagtcc cttggacaac accgccgatc ctctcgggtt tcttagtagg cggagtgcct
     9841 gcagcgattt tacaaggtat cgtgttgtca attagttttt tcatttattt cccatttttg
     9901 aaaaaaatag attctgcaaa ctgcaaaaag gaaagacaga caaaaaatga tggagcagca
     9961 gaaaagatga acgactaaaa aacaaaaata ctcctgtcgc tttagattgg gaaaatcacc
    10021 aatctaggat gacaggagta tttatagtca tgtttatact agcccttttt ggacagctaa
    10081 ttgaacaaga acaactaatg tgttcattcc tacgtgtgtg atcatcgatg tccagatccg
    10141 accagttgct ttgtactcta ggctgaagaa gaaacctagg aaaaagtaga ttagcagatg
    10201 accatcatta tgagcaaacg caaacagcaa agagctgatc actgctccga cccaaaaatt
    10261 tgaatatcga ctgatgatcc ccagtatact gcggcggaat acgaactctt ccatgatggg
    10321 tccagcaaca gttgtcgcca aaataaaagc gggtgcttcc atgatcatct ggatgatatt
    10381 ttgtgtattc tcggaagggg ttgctttgcc aaacaataca gattctaaca tgacagcgat
    10441 gctttgcaga atcaatgcga caaagattcc aaccaatcca taaataacaa tctttggagc
    10501 agaagctttt tttaccgctg attcgatcgc taagggttca cgttgcttaa aataaaaaat
    10561 agctaggaca acggcgccta gtatatacat gactgcagtt gctgtgatca attcagacgt
    10621 agattttaca agtcctatcg aagctaggat caagggtgag ataaaaatta gaccatagca
    10681 aaaaatgctg aggaagctat atttttttaa agacatgtaa ttcctccttt taagggataa
    10741 caagcatgca aggcaagctt atatcgattg atttcaatta tatcataatt tcaataagca
    10801 gctgtcagaa taaaatttct tttctttgat ttaaaagctt gcaaaaaaga gggagaatga
    10861 gtattattaa aaatgtaggt tagcactcga aggttaagag tgctaacaga ttaaaaaaag
    10921 agttatcgga gggatttatc gtgttaaaac cattaggtga tcgcgtcatt atcgaagtcg
    10981 caaaagaaga agaaaaaact gtcggaggaa tcgttttagc ttctgctgct aaagagaaac
    11041 cccaaactgg gacagttgtt gcagtcggtg aaggccgttt gttagaaaac ggtgaaaaag
    11101 ttcctgccgc tgtaaaagca ggcgaccaag ttatgttcga aaaatacgcc ggtacagaag
    11161 taaaatacga aggaaaagaa tatctgatcg tcgcaggaaa agatatcatg gcgattgttg
    11221 agtaaatttg gaagataaaa aaataatcaa aaataatcaa tgaggtgaat caatcatggc
    11281 aaaagaattg aaatttgcag aagacgcacg cgcagctatg ttacgcgggg tagataaatt
    11341 agcagataca gtgaaagtaa cattaggacc taaaggccga aatgtcgtac tagaaaaatc
    11401 ttacggctct ccattgatca caaatgatgg tgtgacgatc gcaaaagaaa tcgaattaga
    11461 agatcatttt gaaaacatgg gtgcaaaact tgtatcagaa gtagcatcaa aaacaaatga
    11521 tattgcagga gacggtacaa cgacagcaac tgttctgaca caagcaatcg ttcgcgaagg
    11581 tttaaaaaac gtaactgcag gtgctaatcc tctaggcatt cgtcgcggga tcgaattggc
    11641 aacaaaagca gcagtcgaag aattgcataa tatctcaact gttgttgatt caaaagaagc
    11701 cattgcacaa gtagcagctg tttcttcggg ttctgataaa gttggacatt tgattgctga
    11761 tgcaatggaa aaagttggaa acgacggtgt catcacaatc gaagaatcaa aagggatcga
    11821 aacagaacta gatgttgtag aaggtatgca gtttgatcgt ggttacttat cacaatatat
    11881 ggtaacagat aacgacaaaa tggaagctgt tctagaaaac ccatatatct tgatcacaga
    11941 caaaaaaatc tctaacatcc aagatattct gccattgtta gaacaaatct tgcaacaatc
    12001 acgtccattg ttgattattg ctgacgatgt agatggtgaa gcattgccaa ctcttgtatt
    12061 gaacaaaatc cgtggaacat tcaacgtagt agctgtaaaa gctcctggtt tcggcgatcg
    12121 tcgtaaagca atgcttgaag atatcgctat tttgacaggt ggtacagtaa ttactgacga
    12181 tctaggttta gaattgaaag atgtaacaat cgaaaactta ggaaatgctt ctaaagttgt
    12241 tgtagataaa gacaatacaa cgatcgtaga aggttctgga gaaaaagaag caatcgaagc
    12301 tcgtgtgcaa ttgatcaaaa accaaattgc tgaaacaact tctgattttg accgcgaaaa
    12361 attacaagaa cgcttagcta aattagcagg tggtgtagct gttgttaaag ttggtgcagc
    12421 tactgaaaca gaattgaaag aattaaaatt acgcattgaa gatgcattga atgctacacg
    12481 tgccgcagta gaagaaggaa tggtctcagg tggtggtaca gctctagtga acgttatcag
    12541 caaagtttct gctgtagaag cagaaggaga cgtggcaaca ggtatcaaga tcgttgttcg
    12601 tgctttagaa gaaccaattc gtcaaatcgc tgaaaacgct ggttatgaag gatcagtgat
    12661 tgttgataaa ttgaagaacg tggaactagg cactggtttc aatgctgcta ctggagaatg
    12721 ggtaaacatg gttgaagcag gtatcgtaga cccaactaaa gtaacacgtt ctgccttgca
    12781 aaatgccgct tccgtttctg ccttgttatt aacgacagaa gcagtcgttg cagataaacc
    12841 agaaccagca gcgccagcag ctcctgcaat ggatccatca atgatgggcg gtatgatgta
    12901 agaaaaagca acttgcttat ctacaactga tcattagtga aagacagata aaaagaggct
    12961 ggaacagaag cgtttaactc caagaaataa gaagaaattc acggaaattg cttttcaaat
    13021 ttttgtgaat ttcagcttat ttccgaagga gttgcttctg cttccgctgt ttatacgtgt
    13081 ttagagcgtg agacaaaagt gttctttact tttgtcccac gctctttttt gtgtgtaatt
    13141 ataatgaaaa tagttatttt taatagttat aatattttat ctattaaaag taaatatcca
    13201 ttc
//
LOCUS       DAKBVG010000069        12729 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000069 DAKBVG010000000
VERSION     DAKBVG010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12729
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.069"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            38..886
                     /locus_tag="OUD09_001356"
     CDS             38..886
                     /locus_tag="OUD09_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCT9692631.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1532..1738
                     /locus_tag="OUD09_001357"
     CDS             1532..1738
                     /locus_tag="OUD09_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HCT9692632.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(1940..2941)
                     /locus_tag="OUD09_001358"
     CDS             complement(1940..2941)
                     /locus_tag="OUD09_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCT9692633.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(2946..4859)
                     /locus_tag="OUD09_001359"
     CDS             complement(2946..4859)
                     /locus_tag="OUD09_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HCT9692634.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(5027..5533)
                     /locus_tag="OUD09_001360"
     CDS             complement(5027..5533)
                     /locus_tag="OUD09_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCT9692635.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            5693..6133
                     /locus_tag="OUD09_001361"
     CDS             5693..6133
                     /locus_tag="OUD09_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HCT9692636.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(6159..7316)
                     /locus_tag="OUD09_001362"
     CDS             complement(6159..7316)
                     /locus_tag="OUD09_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCT9692637.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(7319..7675)
                     /locus_tag="OUD09_001363"
     CDS             complement(7319..7675)
                     /locus_tag="OUD09_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9692638.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(7973..8947)
                     /gene="sgrA"
                     /locus_tag="OUD09_001364"
     CDS             complement(7973..8947)
                     /gene="sgrA"
                     /locus_tag="OUD09_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HCT9692639.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(9143..9982)
                     /locus_tag="OUD09_001365"
     CDS             complement(9143..9982)
                     /locus_tag="OUD09_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCT9692640.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            10167..11054
                     /locus_tag="OUD09_001366"
     CDS             10167..11054
                     /locus_tag="OUD09_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCT9692641.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(11648..12343)
                     /locus_tag="OUD09_001367"
     CDS             complement(11648..12343)
                     /locus_tag="OUD09_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCT9692642.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(12327..12725)
                     /locus_tag="OUD09_001368"
     CDS             complement(12327..12725)
                     /locus_tag="OUD09_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCT9692643.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         3846 a         2501 c         1926 g         4456 t
ORIGIN      
        1 aaaaatgata atcttattat gaaaatgagg aattctcatg agcgtctcca taagactaca
       61 aatcaaaaca atgtacaacg acttcagccc aaaagaacag gctatcgccg attatatttt
      121 agaaaatcca agcaaagtaa gtcatagtcc tattagcaat ttagcaagtg aactgggcat
      181 tgctgactct actttttttc aatttacaaa aaaacttggt tataatggat ttaaagattt
      241 taaaatggcc atgttgatcc aagaaaatga tctatctgct atttctatcc acgaaaatat
      301 tcagaaaaca gatactgagc tcactatggc gcaaaaggtg ttcgagtcta gtatttcaac
      361 tttaaatgac acaaaaaagt tgttacaaca aaaggatcta aaaaaagcgg ctactattat
      421 caatgaatct aaacgtgtct attttttcgg tgttggcggt tcagagattg tagcaactga
      481 cgcttatcat aaatttttgc gttcccctat cgcaacttct cacagcacgg attaccatat
      541 tcaattgatg gaagcttctt tattgacgga aaaagactgt gctgttttga tttctcacac
      601 agggcaatca aaagaaacaa tccatattgc tgaaaccgta agaaaaactg gagcaaaaac
      661 aatcgttatt actagccaag ctaattcttc ccttgctaaa cttggcgatg tcgtttttat
      721 ttctatttct gaagaaacag aattccgatc tgaagcttta gcttcccgta tttctcaact
      781 aagcattttg gactctctat atgtgatttt gatgttttac aacgaaaaag aatcacgtcg
      841 ttctatcgct aaagttcgac atactatttc tgaaatcaaa gaatagcacc ataacagaaa
      901 aagcgaaaaa tccataaaac aaaaaaagcc actaccaaat atttggtagt ggcttttttg
      961 gttctgcaag ggtggtacag aacatcaata gtcggtgggt tagaacgtat gtcatcgcat
     1021 ctttggagaa ggttgtattg gtattattcg agttacttgt tagatgcgat aacaaagtta
     1081 gtatacagta gtaaaaaatt tatcttttct tttttttatt atttttgttt cttattttct
     1141 aaaaaattaa aaaaaatttt ggtgaattaa gtatttcctt gataacactg gcttttggcc
     1201 tcattttagg ttatcatttg taattttatt tcacagttga tatgattgtt atgtaaaata
     1261 aaaaagttta ggatacatcg aatttttggg gaaattcttt tgggggtatc ctaaatcttt
     1321 tctattatat tcccatattt tctattattg gtctagacgt atgatggtaa actttgtacc
     1381 tatctgattg ctgaatcatt aattgcttat cattagccat ctttgtttca agttcgctag
     1441 atgatcattt tattcacatc ctaacaaact tgtcacattt atagattctt aatctattta
     1501 tcatacaatt ataaaaaagg aggtgctgct tatgaaagct atccatattt ctttgctttt
     1561 tttcgctagt gcattgattt tatttgctat tcatctttgt tcccagcctt atatcaataa
     1621 caacggaata ttagtggaac cagcattctt ttgcttacca ctcggctttt tgtcccttct
     1681 tgcctccgca ggcttcggat ttgttgcttt ttttaaacga tcaaaacaac aaatctgact
     1741 tataaaattt acgtaaaaga gatcgtgaca ataatcgtgt gatattccca aatttcgaat
     1801 aaaaggtgcc ccttgccatc tctaactcct cagtcacaag ttacaatatt cataaaccat
     1861 gaaaagcttg tttattgaaa ttgctccttg tgacttggag ctaagtgaca gattacaacc
     1921 tcttttgcat cataagactt tactccttga aaaacattac taacttttga tatgtttctt
     1981 gcgcttgttt attgctgaaa tcaaactgat attcatggtt caaaacagca tttttctccg
     2041 cataatctac ttttgtgact ttcacccccg ctttctcaaa tacagaagtt acttcttcca
     2101 tttgtttcgt aaacgtattt gtatttccat ccgttaagaa tgtgttttgc gtccaacgat
     2161 ccgcatgtct gaccaaggct aaacttttga ccttctctga ttgttcaaag tccttattat
     2221 caaagtaagc ccatccacat ttttcataaa gaaagctaga gattttatca cttacttgag
     2281 taagtgcaga aggatccaac agaccagaca aagaaataaa cttcgtaatc tgcttgttct
     2341 ccaaagtcgg atcaagatga tcagcttttt taatttcttc attttgaagt gctaacaaat
     2401 attctccact aatttgtgca cctgccgaat ttccaccaat cgaaatattc gaccaatctg
     2461 cttgatcttt tatctctttt ttagctgctt gaatggcttc gttcaattgg ataactggtg
     2521 taggatgttt tgcttccggt gatcgttcat aatctatatt tacgatgatt tgctgcgtgt
     2581 ctgaacatag atattccaaa taatcacgac aatcattttt atcccctcct acatatgcgc
     2641 ctccatgcat ccagaaaaga atcggtaatt ttttcttttc cactttcggt gtgtagatat
     2701 ccatattatt atttttgtac tttgaaggat aggatatttc tttttttata tcgatttgtt
     2761 cgattttttg ctggaaatca tctggtcggg catatacttg ttctttttca cttgtgtcaa
     2821 attgttttct aacaagcaag ctaatcggct ttggtgtgat ttgattaatc aaaaatacag
     2881 tgactaaaat aactacgatg ataactggta ctgcaatcac tattcgcttg aaccatttca
     2941 ttcatttacc ctctctttct tcgccaaatc catgtgataa tcgtgctaac agataaaaat
     3001 ccacctgaat aaatgagaag cgaatgtttc ttttctccag ttttcggcaa cattcttttt
     3061 tttgcatctg tctttttttg tactttcact gtcatctgaa tcggttgcgt atcatcactt
     3121 gattgatcct ctcgaggttc attctttccc ttattgtctg cattctctgt cggatcttgc
     3181 ttttttggtg gagctactgg gatttttttg taatagaaaa taatagttgg tatcgtctca
     3241 ttaaaaatcc cttcagtcgg tccttcaaca tttgctaatt cgtatccttc gaatgtcttt
     3301 tgctctgtca tataattttc tccataagct ccgtaaagct ggatttcgtc ctggtctttg
     3361 agtagacttt tattgttttc atccacatac tttacgttta tctgatcatg gaacactact
     3421 ttcaaaaagt ccgttacatg tttcgtgtag gcagcatggt caacttcctg tgcctttcca
     3481 tgccctgctc cttctactaa taaaagatct ttataccctc ctgataactc atataatttt
     3541 tgcgcgttag acacaggtac tacatcatct gctgttccat ggataatcaa tttaggcaat
     3601 gaatctgaca ttccaatcgt aggtagagca gcttttgtat ccaaatgcat ctcctgatca
     3661 aaatagtatt gtttcaaaag gcccataaat aaatcttcat gctcttgatc cccaatgatg
     3721 tctaactgat tttttatggc atccggtagt gcgggggcta ctgtctggct gtacgtaaaa
     3781 cgtagttgtt catcaattga tgcaaatccg caattttcaa taattccttt gacattgaca
     3841 ggaatatctt tcgctaatac agacatcatc gtagctgctc ccatcgaacc accataaagt
     3901 aaaatctttt gagatggccg ctctcgaact tcttgattta tccagtaaag gacgtcatct
     3961 gactcatatt gtccataggt cacataatcg cctccgctat taccagtcgc tctggaatca
     4021 acaaataata aattataccc ggccttataa aaatcattgt attcttctgt aacgatcccg
     4081 ttattccaat tccctcgaaa tccgccatgg atcatgactg ttttatctga accttgatcg
     4141 acataatatg catatacatc catcattttg ccatcttgtt tgtttagtac agtttgatga
     4201 agttgttttg tatttggata gaagcctgtg ttttctgtag gcgttgtccc atcatctgta
     4261 cctccaagca tcgacatcaa cacactgttt tttgcaaaag cgatatcgta caatagataa
     4321 gtagcaatca gatgtttgat aatctcttta tctacaccat aatcattaac agcaagctca
     4381 gtgactaata actctattaa ttttgacttg atgtcttctg ttggtccatt aagtatttct
     4441 gtcatctgtt taccgaagta attcaaatat tctttaccat tgaaattttc acctttaccg
     4501 atatcatcat tttctgtccc attctctttg tcacttactt tagcagaaga ggattcgtcg
     4561 tttgacattt ttgtagctgt tgcttcttct gtttgttggt tatctgtggt agcggtcgtt
     4621 tcttgatctg tgctagtaac agatgtctca accgttgttt cagtatttgt ttctgtttgt
     4681 gaagtactcg atttcgtttc atttccagca tcagtagttt ctgtggtttc tacctcccga
     4741 gattccgatt cggaagaaat gctctctcca tatactgtcc attgcgggac gcttgtactt
     4801 acagcaaata agatgaccaa cgctcccttt aatacttgtt ttgttctcaa gctattcact
     4861 cctttttatg tttctaggag aattatatgt aaacgcaaac attttttata caaaaaataa
     4921 atggtttatc ccataaaaat agaacaggat tattttctca ttttacgtaa taaagcagaa
     4981 tggataaacc agtatccatt ctgctttttt taaacgaaga aacaacttat tgctccaaaa
     5041 atgtcagaaa tcgtttatcc caaattttct cgtaaaaagc gattgtttcc tttgctttca
     5101 taaaaggatt atcataagtt gtcgttgctc ctctacacat cataagctgg tatcctaatc
     5161 catgagcaaa cttcacagtc gtatctatac aatactctgc ttgtgcccca cataattcga
     5221 tcgaatgaca gtccaattca gttaataaaa cactcaaatt agtcttataa aatgaattcg
     5281 catgggtttt tctcacaaaa taactgtgct caggaataga taattccgac aatagctccc
     5341 atggttttgt ttgtggcact aattgagcat cttcgtgttg aacgaaaata actggtttcc
     5401 caatcgtttc gtatgttttg attcttttat tcactaatgc tgtcactgtt tctaattcgt
     5461 gtatcggcga agaatcaaaa catacccctt tttgtaaatc gatcactact attgcgtcgg
     5521 ctgtgtttga catacttgtg ccttctttct tttttcttga tttaatagta tttcttatct
     5581 agctagttcc attatacgtg acaacatttt cttattttac acctctaaat ggcacttttc
     5641 gactatttct tcatgttatt atttcataaa tacttagcta ttggaggaat gtatgatgat
     5701 taaattgtat acaggttctg gctgctcgtc ttcaagacgc gcaaaacgtt ggcttatcgt
     5761 ccatgatata ccgtttatcg aacagaactt aattcgtcaa ccactaacca aaaatgaatt
     5821 tatccactta ctaaaattga caaacaacgg cgtgaccgaa atgctctcta ctcattctat
     5881 tagttataaa aatcttgttc acagaataga tgaactcagt ttgagtgaac tatatattga
     5941 gattcgtaca tccccttctt tattgaaaaa accgattatc tacgaccaat ctgcaaaact
     6001 tttgactgga tttagcgaag ataaaatcag gatgttcctt tctaaaacta aaagaaagca
     6061 agagctcaac gaactcttgc tttctggtca tcttgtcgat tttaaagaaa ataatctttt
     6121 tgcaatgtct taattttggc ataaacaatc tgcttttctt aattaacgaa atccaatttt
     6181 tccaatatgg atctattttt ctcattcctg atttttatga tttcagcttt cagacgcgcc
     6241 caatcatttt ttgtaggttg tccattccag taaaatatct gtgtattcgt ttgatcatat
     6301 gcctttttgt cgataggata atcgctaatg acaatatcag gtaactgatc agcggatgca
     6361 ctatatttta ttgggcgttc agtcatagat aatatccttt tctttgccca taattcctgt
     6421 gttttaccat actttgattg aatatagaat atgacaggtt gttcgtgttt tataaaaata
     6481 tccctgacta acatacagta ttgaaaaatc aaccgtctgt tcttttcata ataagtatta
     6541 agctctggaa ttctgcgagc taccccacta aaaaactgtt gcatgattgg atacatcact
     6601 ggaaatatat gctgaaaatc tctgtcttct gctgattttc caaaagcatc caccttcttt
     6661 tttgtcccaa aaacccttga atacaaatgg atcattgcca aattgacata tagataattt
     6721 ttttctgaag ttgtaaaaca aatcttgaag tttttttcaa aattttctac aatagacgat
     6781 tcaatccagc cccaactttc ttgtacttta tctggagtat ccaacaaatc ttcttccaaa
     6841 taggtgttca ttacaccaaa cataaaataa aggtaagctt gctctgttgg agtgatgtga
     6901 ttcatcgacc agaagtttgc ttcaaaaaaa ggctctagta tgtaatgcgt aaactcttct
     6961 tttgttatca atggatgcac aaatttgttc atctcttctg ggacatacgt aatcgagtgg
     7021 ccttgtttca ttcgttgcaa cgaaattctt agtaggctga tccatttctt tctatctata
     7081 ctacgcgtat atggcgcatg tttcgcaaat ttttctaata gttgacgttt attttttata
     7141 cttgataata tatcagaatg attttcgtcg aatgactgcc aatacatcaa gtgaaaaaag
     7201 tatcggatac ggtattcttc tccttctatc ggattacctt ttttcaagct gcaagataaa
     7261 tgatagtttt tcaaaatagt attaagttct cttaattttc tttgaatcgt acgcaaactt
     7321 atataatttt ttgactaaga ttttctaagg attgataagt acctttgtaa agtgaatcca
     7381 gtaaaatata accaatcgtt ccctctaaaa gtatagaata aactttactc agtaaaaact
     7441 gagaagaaaa ctcgactttt atcatcttgt tttctgaaag aaaagataat ttatattctg
     7501 gatacctttc gcaaaacgat tctgcttgtt gaatcaccga attcatctta gccagggtgt
     7561 agtttagatc taaagcgata tccttcacca catataattt ctcgtctctt tcataacaat
     7621 agttcaaaaa ttgatatact tgcctttcat tgatatcaat aaattcttca aacaaagtac
     7681 atactccttt ttatctatta tttagactta ttccgacagc tacatgaata acgtacaaat
     7741 agattcgtta ctgatttttc agagatacta ccatgcgcag tgtgcattgt caattattct
     7801 tgctcttatt ttccatttgt gttcctgtta tttcctgttt ctcaaatatg ttagtaaaca
     7861 aaaatagatc tggcaaagct acaacttcgc caggcaatac agcactctcg aatgaaatat
     7921 tcactcaaaa atctatcgtt ttattcaatt ttagatctgt tgcttttatt ctttatttca
     7981 ctcgatggaa gagagaacga aaatagcttg ctcctacaat caaaatgagt aacacaccca
     8041 tccatgtaat tagcttgttc aaggttctac taccagtttc tggtaaatcg ctttttctat
     8101 tatctgtttc tttcgctttt tcttgatgat caattccacg agaaacaaaa tcaggctgtt
     8161 ttagcacact ttttaggttg ctccctgcat ttctcttttg ttccttagtt ggtatgatag
     8221 gtaccaaatc aagttcatta tttacttttc ctgtgctgct ttcgctgctc gtgcttcctg
     8281 tgctactctc actactcatg cttcctgtgc tactttcact actcgtactc tccgtactgc
     8341 tttcgctgct cgtgcttcct gtgctacttt cactactcgt actctccgta ctgctttcgc
     8401 tgctcgtgtt ttctgtgcta ctttcactgc tcgtactctc tgtactgctt tcaccgctcg
     8461 tgttttctgt actgctttca ctacttgtgc tatccgtact gcttggcggt ccaacaactg
     8521 tccaactgct tggtttcaaa ttctccgtga tcgtcatctg tccatcataa aaaacaatca
     8581 aatcaacttt atctttactc ttgatctcat caatcgatag ttctgcagaa atcattattg
     8641 tatactcaat accagcggtc cattgtacgt cttttaaata aacttcccca ataacttttt
     8701 ctgaacttcc attaccatcc caaccatatg ttagaacagc atgtacatca ctagcattca
     8761 cgcttttgtt tggtttgact ggaatagtaa tgacttgttg tgtcatgtca taaacaatcc
     8821 gatcagtttt cacagtaaat gaaagattct catttgcccg ttcatttgca gaagtactga
     8881 ttccaatcat tgctgcaatt atactcagcc caagaataac aaataatacg gtcgctgttt
     8941 tcttcatttc tatcactctc catataatta ccttctattt gataaaggaa ttatataaaa
     9001 tgaaaatgat gagaacacga cagtcagttg tgaaaatcta acaaaagatg tcgtgtattc
     9061 gcttgaattt gtttactttt tcgtttaatc aatgacagac ttaaaaaagc aagaccgata
     9121 tcagccttgc tttcttcatc ttttattgat aaacagcagt gtatttcttc atccgctttg
     9181 ttttatatgc atagagtaac aaaccaacaa tcaagactaa caagaataat gtagttgata
     9241 tagatgcttc agccccaaag tttcccccag taattattgt attttgcgat tggatcagag
     9301 tggagaaaaa accttgttct tgcgttccgc tgactggtat acccaaaata gctccttgtg
     9361 tccaattcca agtaatatga gcaccaatag ccgtaaataa acttcctgta ctgtagataa
     9421 gtgcgctgaa aaagcaacct gctaaaaaga tattgaaaac tgccagaaat gtcatgttag
     9481 gattcaatcc gtgcaaaaga gcaaataatg cagcagatcc aatcattgct attaacggac
     9541 caaatctaag catctgatgc tgaaaaaagc cacggaataa aagctcttct gatgccccca
     9601 ctgcaataaa taaaataaaa ctcgttatca gcggcacaat gtttgcgttt cctgaatcta
     9661 accgatacgc ccccgtcaaa tataatggac tagtaataag cagaaacact ccaattccaa
     9721 caacgagacc tcccgctaaa tacccccagt cttttcctcg aacagaaaca ccataatctt
     9781 ctattctttt tccttcgacc ttactagcaa gaaataaagc tagtaaaaaa acagctaaat
     9841 gtccgaccgt accggcaatt gctccaatcc attcactatt tccaataaac atatccgcaa
     9901 gtgcattatt gacaagaatt cctaaaacaa aaccaactaa gactattaaa acagatccaa
     9961 tcaaccgaat aatcctgatc atctttcatc cctcctttat attaagttta acaacccttt
    10021 tctttcttgt ctcgtgaaaa ctatgtaaat gttctccatc atagagagaa tagattttta
    10081 cgaactaaga atatctacag caaccttacg aaaatgaaag actgtctccc taattcttct
    10141 gttagactgt ttttaataag gaaaggatgg gaaatttttt gaaaaaacaa ctactaatta
    10201 tgtgcctttc agcatgtgca attttactat tctcaggttg tagcaagtcc aatgaaacga
    10261 ttgcaacgtt taaaggtgga acacttacag ttcaggattt ttatgataac ccaaaagtta
    10321 aatcaaataa tcaaacacta ttaaaaaaaa tgattgtctt caaagcattt gatgatgtat
    10381 acggaaaaga gatttcttca aaggaagtaa caaaagaata taatgaacaa atcaaaaaac
    10441 tcggtccaaa ctacaaggaa caattaaaag cagttggcca aacagaagag acatataaga
    10501 aactctttaa acagatgctg gctttccaat acggtttgaa agccaatgta aaattaacag
    10561 ataaagatct agacactgct tggaaagaat tctatccaga agttagtaca cagatcattc
    10621 tattttcgac taaagaagaa gcagataaag caaaaaaaga agcaaatgaa ggagaaaatt
    10681 tctccaagct tgttcaagct tatggaaaaa ataaaacatt aaaggaaact gatggaaaaa
    10741 tgaattttga ttcaaccaat ccagagattc ctactgaagt aaaaaaagca gcatttaaac
    10801 taaaaaacgg ggaagtttct gatatcattc ctgtaacaga tccaacgact tatcagcaaa
    10861 gctattatct agtaaaaatg gtaaaaaaac aggataaagg ttctaataaa gacaaataca
    10921 aatctgagct agaaaaaatt gctaccgaag caaaaactct tgatacggaa ttcatggata
    10981 aaacaatccg taaagtgatg aaaaaagaca atgtaactat taaagatcct tatgtaaaaa
    11041 atatattcaa ataaacaaat ttgacccaat tggggatgtg gcaaaatttg agaagtgatt
    11101 tttggaaatt ccttcttatt tttgaggcaa accacttttg ccacaacttc atcggggcct
    11161 ttttttattt gtaccaaaac aaatcactgg atttgttaca atcatgagga tataatttaa
    11221 aaaagttttt tatttctatc acatgaatca atgcgccatt aagtagattc tttcatgctt
    11281 ccctattttt tcacttgccg tatcgcttca aaaacaacta tcataaactc aaactcgact
    11341 gaaaagttat tttaaaaaag actaaaaata tcccttctgg acttgttctt cttattctgt
    11401 tatactagag aaacacgaaa gatattttta tgcgacactt ttcatttgtt tttatgaaat
    11461 ttattcaaaa ctggcgtaac ttcagagtta caaggatgta ttagaggtta gggaatacat
    11521 cgtttaaatg agaaataagt aatagttagt aatagtcacc caaactttaa attaatgaat
    11581 attgaatcgt cagatagcct cctcgagttt tcgagaaggc tatcgtttat tttatggaga
    11641 aattgattca gaagtaatca gcgacctttt ttagttcacc aatcgtttct gcttggaaag
    11701 tttgcaccca atcttcttgg aagcccctca ctgctttatc aatcgagggc atctctaata
    11761 cttgatctag tagctcttta cctaacgtta tcgcatgagc tccagatcga atggctgtag
    11821 tgacttgttc acaatttttg aaactagctg ctaatatttt tgttggtctt tgcgtacgtt
    11881 cgatctcagt ccgcaacgaa tcaatcactt tcatagcatc aatgccttga ttttgcatcc
    11941 tgttataata aggtgcaata taatctgctc cattattaat agctaaatac ccttgtaatt
    12001 ctgtgtaaat agctgtaccg gtaatcaaat aaccttcttt tttcaacttt ttgatcgctt
    12061 gaaggcctgc ttgattcacc ggcaccttga taaacgtatc ttttcccagt tcttctatga
    12121 taactcgagc atcctctatc atttcttctt ccgttcttcc tattgtctga atatggatag
    12181 gaatctgtcc gattattttt ttgatttctt tcatatgcga gaagaagttt gacgctcctt
    12241 cttttttcag aattgtagga tttgttgtta cgcctgttaa attcaaaaac tgttgacaag
    12301 aataaatact ttttgtattt gctgtatcaa gcatgatttc catatagctt tcccccattc
    12361 tgatcctctt gatcgatttt aatgattgtc ccctttctta aatgcggcat ttccttatct
    12421 tctacataca ttgttcctgg aagttccggc tcatgagatc catcaaattt tatcgtgata
    12481 tgtcctaatt cattcaaatt agtttggaca acatctccta ctgctgtgat tacatacatt
    12541 tgtttatcaa aacagagctt catatcacaa gttatttctt ttcttacctg attcaattca
    12601 atcttataac aataatctgt taatgtttcc ggtgcttcat ctccaaataa aatcattatt
    12661 ttttctttcc tgaatacttc tgcttctata ccaacatctg ttacaaccgt ttcaaagatc
    12721 gtcatctct
//
LOCUS       DAKBVG010000070        12607 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000070 DAKBVG010000000
VERSION     DAKBVG010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12607
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.070"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(52..1200)
                     /locus_tag="OUD09_001369"
     CDS             complement(52..1200)
                     /locus_tag="OUD09_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HCT9692644.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(1322..1918)
                     /locus_tag="OUD09_001370"
     CDS             complement(1322..1918)
                     /locus_tag="OUD09_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCT9692645.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(2118..4760)
                     /gene="alaS"
                     /locus_tag="OUD09_001371"
     CDS             complement(2118..4760)
                     /gene="alaS"
                     /locus_tag="OUD09_001371"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCT9692646.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            5148..5579
                     /locus_tag="OUD09_001372"
     CDS             5148..5579
                     /locus_tag="OUD09_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCT9692647.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(5624..6973)
                     /locus_tag="OUD09_001373"
     CDS             complement(5624..6973)
                     /locus_tag="OUD09_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCT9692648.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(7043..8206)
                     /locus_tag="OUD09_001374"
     CDS             complement(7043..8206)
                     /locus_tag="OUD09_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HCT9692649.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            8335..8658
                     /locus_tag="OUD09_001375"
     CDS             8335..8658
                     /locus_tag="OUD09_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9692650.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(8841..10190)
                     /locus_tag="OUD09_001376"
     CDS             complement(8841..10190)
                     /locus_tag="OUD09_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCT9692651.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVIAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(10259..11212)
                     /locus_tag="OUD09_001377"
     CDS             complement(10259..11212)
                     /locus_tag="OUD09_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HCT9692652.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(11314..12504)
                     /locus_tag="OUD09_001378"
     CDS             complement(11314..12504)
                     /locus_tag="OUD09_001378"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCT9692653.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
BASE COUNT         3612 a         2664 c         2196 g         4135 t
ORIGIN      
        1 aaaagactct ttaaattaaa aactttaagg agtcttttta tagtttctca tttaatataa
       61 aagataattg tgatcaaacg taatttccaa ttggtaatct ccgatttttt cttctatttg
      121 actacgtaag attctttcta gttctgtatc aacaaactgg aatttctcag ggaccactac
      181 atcaaattgc agcgtttttc cttgatgaat tactctaaaa tcatgaatat taagccctgt
      241 acctgtttca agaagaattt gctttaactg ccgcaagata ttatagtatt tttcgttatc
      301 aacaggcatt ggatccaagt gacagacaag ttccacatcc aaagactttt taaagtcttt
      361 ttctatcaga tcaataatct tatgagccac attcaaattc attgattcat ctacttcgat
      421 gtggactgat gcaaagcgtt tattgggtcc atagttatgc accaataaat catgatagcc
      481 aagtatatca tcgcaagcat ttaaatgctc ttccatttct tcaatctctg ctggcgttgg
      541 gcgacttcct aatagttcgt atacaaaatt tcgaagcatc gtcaatccac tataaaggat
      601 atataatgct aaagcaaaac ctatatatcc atcaatacgc catccagtta cccattcgat
      661 tgctgctgaa agcaatacag ccagtgtagt aaagacatca ttataactat ctgttgctgt
      721 agctctcaat gtttcagaat caatggattg cgcaataacc tgatacattt tgccttgtgc
      781 aaatttcaaa aagatggaaa tgattaggac aaaaaagacg atgggtgtta aagagacatt
      841 ttctggatgg atgatttttt cagcagaaga ctctaaaaat tgaaatccta cataagtaat
      901 gatgatcgaa acaaataacc cactgatata ttcgaagcgt tcgtgtccat aaggatgttc
      961 ctgatctgct ggctttgcag caattttaaa tccgatcaaa gtcaatatgg atgaagcagt
     1021 atctgataga ctgttcactg catcagccat aatggagaca cttcctgaaa aaagcccgat
     1081 cattagctta gagacaaaca agatcagatt cgtaaccaat cctaagatac ccgctagtac
     1141 tccttttttt gtccgctgtt gcggtatttg tttctttttt tcttcttgtt tcttagccaa
     1201 gttttttacc ccttctagtt tgcttgttgc tcaggtaaaa agatggagag gacgattgtt
     1261 ttcctctcct ctcttccttt ttctatctat cgtgcgtcct attactatta aaagatggtc
     1321 attattctgg tttcaacggt gttgctgcaa ataacgcttt tgctagtaat ggtgttatga
     1381 ttcccgcacc tattgcttct ggtatcccat tgattgctgc aacccctgct aaggttttaa
     1441 ataaaagagc agagtctaca ccataggcat ctgccgttgc acctgtataa aataatccca
     1501 tgaatcctaa cactaagact gtattgacca atgaacccag tacccctgca aaggctgagc
     1561 tgatcgttat tgatttattt ttttgataaa tccagtggaa aaccaagcct gctactaagc
     1621 caacaagtac tctgggaact accgagataa tcggattcgt gaacactaat gtatcaaatg
     1681 gcgttgttgg catggcatag gcacgaacaa ttgtagcgat tccccaaaac aagccaatca
     1741 ccatgccatc ttttgggcct aatactactg cagcaataat tactgtgata tggataattg
     1801 ttaaactaat gaatcctaat ggaataaacc ccagccaagg aaccatcgct tgtacgatga
     1861 tgattgctaa caagatcgca cgtaagacta gtcgaaaggt cttatttcta ctattcattt
     1921 tttcacccct ttttatttat actaatcgta aaaatttctg tcactttcgt caactaaaga
     1981 ccatccgaat ccgaccattt gattagatgc gccatccaat tgaaaaaagt atgagggcaa
     2041 gacttgtttg tcttcgcccc cataatcatg tttcgttcaa tgtttgaact tttgtttttc
     2101 tgtcaaacgt aaacgcccta ttgttgcgca acccattctt tcgcagcttt aagtgcatct
     2161 ggaataccag caggattttt cccgccagct tgtgccatgt ctggacgtcc accaccgcca
     2221 ccgcctactt tcggtgcgat tgctttaatc aagtcgccag cttttagtcc agcttcattt
     2281 gattctttcg tcattgccgc tagcagactg acttttcctt cttgttcgtt tgctaaaaca
     2341 agaatatctg aaatattttt ttgtttccat tggtcagcta attgacgcag ctgattcata
     2401 tctttcactt ttacttgtgc tgcaatgatt gtttttccat tgatcgtttc tggatctttg
     2461 aatacatcac cagcttgttg attcgctaac ttgctagcta attgttcatt ttccttttgt
     2521 agctctctta cttgctgttg caattgttcc gcttttgaaa caacttcttt caattgaggt
     2581 gatttaacaa ttgttgctac ttgttttagt tgtttttctt cttcttcaag tagttcgtac
     2641 gcttctttac ttgttacagc ctcgattcgt cgcacgcctg caccaattcc tgattcagaa
     2701 acgattttga agattccgat gttttctgta tttgctacat gtgtgcctcc gcacagttcg
     2761 attgaccagc cgccaatatt tactactcga acgtcatgtc cgtatttttc accaaacaaa
     2821 gccattgcac ccatgttttt ggctgtatcg atatctgttt caattgtttc aactgggata
     2881 gcttcccaga ttttttcgtt gacgattctt tccatttcag ctaattcatc tgaagtgatt
     2941 tgaccaaagt gggtaaagtc gaatcgtaaa tgtccaggag cgactaaaga accagcttga
     3001 tttgcatgct cgccaagtac ttctttcaat gccttgtgca gtagatgggt tgctgtatgg
     3061 tttttgatga tccgattgcg catcgattga tcgatcaata gatgataggt ttgtccttca
     3121 ctcattggtg cttttacttc tacggtgtgc ataaattgac cattaggcgc tttttgaaca
     3181 tctgtaacaa ctgctacgat ttcgcctact tcattcatga tccatccttg atccgctact
     3241 tgcccaccca tttctgcata gaaaggtgtt tgatcaaaga tcatttgagc ttcaccttct
     3301 gcgacttcat tcaaaagaga ctcaccttta acgatgacca ataattgact atctgattct
     3361 tgttctgtgt atccaatgaa tttactttct actttgatat ctgtcaataa agcagattgt
     3421 acccccattg atttttcagt actgcgggca gagcgagcac gttcgcgttg tgcatccatt
     3481 tctttttcaa aaccagcatg atcaactttc agtccttcat cttctgccac ctcttccgtt
     3541 aattctaccg ggaagccata tgtgtcgtat agtttgaaga tgtcttttcc tgctagagtc
     3601 gtcccgtttt cgttttttac ttcttggatc acttgattta agatttccaa accttcattg
     3661 attgtttcat tgaaacgttc ttcttctgta cggataactt tttcaataaa ttctttttgt
     3721 tgaagtactt caggataata acttaccatg atttccccaa caacagggac taaacgatat
     3781 aagaatgctt gttcaatacc taattttttc ccatgcatga cagcacgacg aagcaaacgg
     3841 cgtaagacat accctctccc ttcattcgat ggcaatgcac cgtcgccaat agcaaatgaa
     3901 agggcgcgaa tatgatctgc gattactttg taagaaatat ctgttactgg gttttcacca
     3961 tattttactt gtttgcttag cttttctacg gcgtggataa taggcataaa caagtctgtc
     4021 tcaaagttcg ttgacgcatc ttgaacgatt gagaccatgc gttccaatcc catccccgta
     4081 tcaatgtttt tatgaggaag tggttcaaat gtgttatcag gcttatggtt gaattctgaa
     4141 aatactaagt tccagatttc taaataacgt tcattttctc ctcctggata gttttcagga
     4201 tcgtcttctg ctaaatcgtt ataagcttct ccacggtcat agaaaatttc cgtatccgga
     4261 ccacatgggc ctgcaccgat atcccagaag ttgtcttcta catcaataat gtgatctagt
     4321 ggcaggccaa cttcttcatg ccagatgcgt tttgcttctg tatcttttgg ataaactgtt
     4381 acataaagtt tttctggatc gaatgccatc cattttggat cagtcaaaaa ttcccatgcc
     4441 cagtggatgg cttcattttt gaaatagtca ccaatcgaaa aattacccag catctcaaac
     4501 atcgtatggt gccgtgctgt tttaccgaca ttttcgatat catttgttcg aatagatttt
     4561 tgggcatttg tgattcttgg attttctggc acaactgaac catcaaaata ttttttcaat
     4621 gttgcaacac cagagttgat ccacaataag gttggatcgt tcacggggat caaagacgca
     4681 ctaggttcaa tcgtatgtcc tttagattgg aagaaatcaa gaaacatttg tcgaacttgt
     4741 gcactactta attgtttcat gtcattacct gctttctata aattaaatac aaaaaaacac
     4801 catcacagcc aaggacgaaa actcgcggta ccaccttgat tgcaatgatg tgtaatcatt
     4861 acctcttaaa ctcgataacg ctgagctgcg tcggtatttc taccaaaata gaaaaaggga
     4921 gcaatttgat tagtgactat tcttttttca gccaagaaag aattttctgt gagtcttaaa
     4981 aaacaaatca tatccttttc gtacttaatt agtatacgca ttctattgat cactgtcaat
     5041 atctcaattt ttattttcat ttccttttgg tcatactcca cgatttttgg tacgataaga
     5101 ataaaggaat caatacatct ctgctattca tgacaaggag gaatatgatg aaaaaaatag
     5161 ttggagcgga tgaatggata cgcgcagcta gctgcttttt gagatatcaa gtttttgtat
     5221 tagaacaagg gattcctgca gaactagaat tcgacggaaa agatatcccg caacaaacct
     5281 attatctatt tcttgatcaa aaaagaccag tagctacttt gcgttatgaa aagaaaacac
     5341 ctacttgctt caagccggaa cgattttgcg tagcaaaaga ataccgagga aaagggttag
     5401 gcggtcagtt gcttgaagct gctgaaaaac gggcggtttc agaaggttgt cagactagtt
     5461 atgtagtagc tgaaatgacg gctgtagatt tttatgaaaa acaagggtat catacttgca
     5521 cagaaccttt tattgaagat gaagtacttt gtatcggaat gaaaaaaagt ctagcttaaa
     5581 aacaagagat tgtgacacgc cttttgtcac aacctcttgt tttttattta tgtgaatttt
     5641 tcttattttt acgagcagta cggctttgct gtctgcgttc gattttgcgt tcttttttat
     5701 ttttttctgc aattgcccaa tcgatttttt tcttataacc aggcttgatc tttttctttt
     5761 tcttcttcac aagaccaatc agcgtgggat ctaatacatc ttttgatttt tctcgtttgg
     5821 cacgtctatt acgatcgtat gttttgacga tttccccgtt tttgatttct tttggttcaa
     5881 atgagacgcc caatttttct atcgcatcaa tggcttgctc atcatttggc gaatataacg
     5941 taatggccgt tccttttaag ccattacgtc cagtacgtcc gacacgatgg ataaagaagt
     6001 ctagatcttc tggaatttct gcattaataa catgagagac accttcgata tcgatccctc
     6061 tagcagctaa atcagtcgct acaacatatt gatactccag attctgtact tgtcgcatca
     6121 ctcgttttct ctctcttggg gtgatgtctc catgaatctt tgctactttc aagccttggt
     6181 ttttcaagta atcggcaatt tcatccacat gctgttttgt attggcaaat acgattgcta
     6241 aataaggatg accaattgtc agcagttgat aaatcatttg attctcatct ttaccttttg
     6301 ttgaaatcag ccaattttca atgtcttcag aaataactgc ttttggtttg atttcttcaa
     6361 tgattggatt ttccatgtat tttcgtaaga atggacgaag tttttctgga atagtggcag
     6421 aaaaaactaa aaactgcagc ttttctggta atcgacttgc aatttggtct acttcattta
     6481 aaaaacccat atccaatgtc atatccgctt catcgacaac aaatgcaaat gcggtgtgaa
     6541 tcttcagcgc ttgttcattc atcatgtcta atattctacc aggcgtgcct actacaacct
     6601 gtggctgttg gtgatgcaga cgatttaatt gacgctgttt gtctgtaccg ccgacaaaat
     6661 tcgagacacg gatctccggt gtagaaaatt tagcgatttg tgtagctgct tcatagatct
     6721 gtgcagctag ttccctactt ggagctgtaa taacaacttg tacttcctct ttttctgcat
     6781 tgattttttc aaacaaaggc aacaaaaacg tatgtgtttt tcctgtacca gtttgagatt
     6841 gcccaatgat attttttcct tttgcaatta aaggaatcaa acgttcctgg acctctgtgg
     6901 gctgtttgaa gccctttgcg gttaacgctt cattgataaa cggtttgaag cggaatatat
     6961 caaaattatt cattcgtgca cctcttctct ttaaattatc acgactttgg cattataaca
     7021 cagacaaagc gtttttgctc agctaatttc gagaatcatc atcctttgtt tggccaatcg
     7081 tgctaacagt atatgtgtga ctagcttttt cataaaacag aatccgtttt tttctcaaaa
     7141 atgatagcaa gatatttaaa ataaaaatac cttgtagtaa caagacaccg agagaaatga
     7201 atactgcact catctgcaac accgtattcg ttgtcgaatt catgatttgc tgaagcaact
     7261 gccccaacca gcttaaacca taaaaaagaa agtacagata cccgtagcgg atcatcaatg
     7321 cttttaaaga aattcgttcg cggtcttctt ccaccaaacg gatacgcaca atttttttgc
     7381 caagtgtcca tccattggtt ataaacggta caatcataaa gtatacccct acttctaata
     7441 aaagccacca gatatgagaa gtaactgccc ctgaatcgaa cgcaatttta aagaaaatcg
     7501 ccgctaccaa attaataaga gataaaaatg tccagtcgat caaataagcg accaagcgtc
     7561 gagtcaatga gacagtcttt cctttttcat aagaaagttc atccattcgt cttcttgtag
     7621 gaagcataaa tgtaaataac ggcgtaacga caaaaccaat gattccaccc aatgtatttg
     7681 tcactaaatc attcacatca aataagcggt atggtcttgg gtagataaaa taaagtccac
     7741 ttagctgcgt caattcaaaa aatagagaaa ggcagaaact agcaatcatc gttttcataa
     7801 aagaaagccg gaaataatat cttaaataaa tgccaaatgg tatgagtaat aaaacattaa
     7861 aagctggttc caaaaataca ctctgcttca ttgccggcag gtaggtacta ggttcagaca
     7921 agaccaatac cgtgttgtct ctgaaatgtt gccatgaagc aaataaatgc agctccatct
     7981 ttggccctgt catttgggca accgtctcct ttgccggaag tggtaagatc accaaaaaat
     8041 acgcacacaa taaataaaaa acgaaagaat acaaaataac tgcccttgtt actagaaatg
     8101 ttccatactt tcgatattca taaataaaaa aaacagatga gatcgctaag gctaaaaatg
     8161 gaaaaacaat cagcgcaaaa cgaatcggtt cgatataact tgccatatga ctcctccttg
     8221 attctttgta tctctcttat cttagcagtc ctttgctgtt tcaagcaagt aacgaggcta
     8281 ttatttctca tttctttcaa agtaaagtaa gataaacaca aagggggtgt cgagatgctg
     8341 aatatgtact ttgtttttgg cgtacctatt tttttactgt tcctttatgc tacgatcgct
     8401 tatgtaagaa aaagaactac aattcattat ttaggattta ttctcctgat tatttcgggt
     8461 ttcatgctag tcttcaatct tcagacttgg cagcaagcac ttcttgaaat ggataaaatg
     8521 actccacatg ctttgtctaa agtgcttggc taccctgtct atctgatttg gcttcccatt
     8581 ttcatttctg gttgcttggt tttattaaat atttatcgcg gcgtacgccg aatagttcag
     8641 ttacgcaaat caaagtaaaa gacagaaatc ggttgagccc gatttctgtc ttttactttg
     8701 ctatttcatc gtagacaaaa cgacatttac gtgatcaaaa ttaacgagta cttttgacat
     8761 ttcttccacg tactcctcat ttaaataaaa tattttgttt ggcagagaag tgcagttcta
     8821 ataaaattcg aatgttttag ctatctacgt tttaaataga gataaatcgg gataccagca
     8881 agtgtcaata ctatccccat cgaagctaaa aaggtctgag tgataagcgt gcttgatacg
     8941 ataaaagtcc ctccaatgat tgctactagt ggaataaacg gatacattgg aactttgtat
     9001 ggtcgaaaca aatcaggttc ttttttacga agaagaatga caccaacaaa gaccatcgta
     9061 taaaaaatcc aaatgacaaa gatcagcata tccgttagtg tatcaaaacc accgatcatc
     9121 atcatgccaa tagcgatgac tagctctaag atacctgcgg ctacaggaac tttagtttta
     9181 tcatgaagtt tagcaaagag cttgctaaaa ggcaggttgt tttcttttgc aataacataa
     9241 gggagacgca ttccagtaag ggtatagcca ttgatggttc cataaacaga aatcaagata
     9301 ccaactgtga ccagtcttcc accgaagccg ccaaaaatca tttttgcaac gtcacttgcc
     9361 gcattgctgt ttcctgcgac tccgtcgata gatgcggttc ttaagaacac cgcatttact
     9421 aataaataaa cgatcatgat tccgatgatt cctaaagaaa ttgcttttgg caaatctttc
     9481 gccggctttt tcaattctcc cgaaatattt cctacgtgta tccaaccgtc gtatgcgaac
     9541 atcgttgcta gcaagcccgc tcccaaggct gagaaaaatg aaagattttc tcctgcttgg
     9601 atcggaaaca gttggaagtc tactccttct tgtcggaaca aaccgaaaat gacgatcaca
     9661 aacaaaggaa taagcttgca gacaagcgta atcgactgaa aagcccctcc agctttagaa
     9721 cctaaaaaat tgatcaacag gatcgataca gcagctgtaa cagctacggg aacaatcatt
     9781 gactgggaaa gaccaaatag attgacgaac tgtgtaccga aaatgatcga caaggctgct
     9841 acatttgctg gaaaataaat cacgacttgc gcccagccca ataaaaaagc tgctgtattt
     9901 ccgtaaatcc gttcgatata tttaatcatc cctcctgttt ctggaatagc agctgccagt
     9961 tcagctcctg ttagtcctgc acagacagaa atcataccac cgagaaacca agaaaacata
    10021 tgcaaactgg cggatcctgt aacttctgct acgcttgcgg ctttaaaaaa tacccctgca
    10081 ccgatcactg tgcccataac agtcgataaa gcagtaaaaa atcccatcgt acgttttaat
    10141 tcattttcta tttttacttc ttttactggc gtggcttgat attcttccat cccttgtccc
    10201 ctttcatacc atagccaatc ccaaaagtat aaaaaacttt ttggatgtgg ttcttgtttt
    10261 ataccgtctc taccaatttt tctttcagtt tttccaacat atcctttgtc atcacatcta
    10321 agtcgtattt cggcgaaaag ccccattcct cttgcgcaca agaaacatct agtgaatttg
    10381 gccaagattc tgctattgac tgacgtacag gatccacatc ataatccatt tcaaaatctg
    10441 gtatgatttt ttgaatagat gctttgatct cttccgggtc aaaagacatc gccgatatat
    10501 tgaaggcatt tcgatgaacc aatcgatcag gatctgcatt catgagtttt ataatggcat
    10561 cgatcgcatc tggcatatac atcatatcca ttgatgtacc ttgatcaatg aaactagtgt
    10621 actttccttc tttgattgct gaataataaa tatcgactgc gtagtctgtc gtaccgcctc
    10681 ctggcaaggt tgcataagaa atcaatccag ggaaacggac accacgagta tctacgccat
    10741 atttcacata ataataatca catagtagtt ctccagctac cttcgttacc ccatacatgg
    10801 ttgtaggacg ctgaagagta tcttgtgggg tatgatcttt tggagtattt ggaccaaacg
    10861 cgccgattga acttggcgta aaaaatttca gctggaattc tctagcgact tccaatgcat
    10921 tgaccagccc agtcacgttc aaattccagg ctaattgtgg ttttgcttct gcaacagctg
    10981 acaaaagagc agccaaatga atcaatgtgt ccacttgata ttgttctgct atgctgcgca
    11041 ttttttcgta atccattaca tctaggatct caaagatatc gtttttctca atgacttcag
    11101 gcatgcgtat atctgtcgca atgacatttt cactgcctag ttcttgacgt agccgtgtga
    11161 tcaactctga accaatttgt cctaaacatc ctgtcaccaa tacttttttc atgatgatcc
    11221 tccctgatct tttttactac tatcttcaga tgattaacaa tatatgcgtg aacatgttct
    11281 ctaattttaa atagacgaaa aaactaaatt tagttaaata atttccattt cttttcctat
    11341 tttttgataa actgctactg cttcatcaag catttccctc gtatgttccg cagtcggcat
    11401 atttctgatc ctgcctgtcc caagaggcac agttggataa acgattggtt tagcataaat
    11461 cccatgttcg atcagcttct tagagaaagt ctgagtcact ttttcgtctc ccaaaatgac
    11521 tggagtaata ggtgtttcag acataccgat gttatagcct acttttttca attcttcttt
    11581 aaaataatta gcattttccc aaactttttc gacaagttcc ggatgttctt gcatcaaagt
    11641 aatcgaagcc aatgcagctg cagctgcacc tggagttagc gaagtagaaa ataggaatgg
    11701 ccgacttcta gctttcaacc agtcgattag tgttttcgat ccagctacat aacccccaac
    11761 aacacctata gctttcgata acgtgcccat ttgcatatcg attttgtcgc ttaatccaaa
    11821 atgcttgacg gttcctgcac cttttccagt tacgcctgag ccgtgcgcat catcgacgta
    11881 ggtgatcagt ccatattttt caacaatcgg aataatttcc ggcaatcgtg caatgtctcc
    11941 atccatggag aaaacaccat ctgtgatata catgattttt ttatatttct tactttctat
    12001 cgcttccttt gcttttttct caaggtcttt catatcttga tgtttaattc ggatgatttt
    12061 tgctccggat aagcggcaac cgtcaataat agatgcatgg tttaattcat ccgataagat
    12121 agcatcttct tttgtcatta ctgcagagat agcgcccata ttgcagttga atccagattg
    12181 aaaggcgatt gctgcttctg tccctttgaa ttctgcgatt ttttcttcta attgctgatg
    12241 gattttcagc gaaccattga tcgttcggac tgctcctgct cctacaccat aagtttcggt
    12301 tgcttcaata catgcttttt tcaattcttc gcgattggca aatccaagat aattgttgga
    12361 tgctagattg atcatctttt ttccgtctac gatgatacaa gcaccatttt cactttctag
    12421 tatgtcgatc gtattgtata accccttttc tttcagttga tccaattctt tctctaaata
    12481 cattgttagt tctttggaag acattgtctc cacctccata ttcatctgtc ttgcagaaca
    12541 taccaaacat atatcgtgtc attcatctac aaagcaagta tgctatattt ttgataacgc
    12601 ttacact
//
LOCUS       DAKBVG010000071        12537 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000071 DAKBVG010000000
VERSION     DAKBVG010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.071"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            131..451
                     /locus_tag="OUD09_001379"
     CDS             131..451
                     /locus_tag="OUD09_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9692654.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            903..1103
                     /locus_tag="OUD09_001380"
     CDS             903..1103
                     /locus_tag="OUD09_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCT9692655.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1250..4039
                     /locus_tag="OUD09_001381"
     CDS             1250..4039
                     /locus_tag="OUD09_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCT9692656.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4086..4613
                     /locus_tag="OUD09_001382"
     CDS             4086..4613
                     /locus_tag="OUD09_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HCT9692657.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4634..5824
                     /locus_tag="OUD09_001383"
     CDS             4634..5824
                     /locus_tag="OUD09_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HCT9692658.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5864..7162
                     /gene="asnS"
                     /locus_tag="OUD09_001384"
     CDS             5864..7162
                     /gene="asnS"
                     /locus_tag="OUD09_001384"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCT9692659.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVPEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            8328..9002
                     /locus_tag="OUD09_001385"
     CDS             8328..9002
                     /locus_tag="OUD09_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HCT9692660.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECVNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQKAGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            8999..9340
                     /locus_tag="OUD09_001386"
     CDS             8999..9340
                     /locus_tag="OUD09_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9692661.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLI
                     AVTKEKQIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTKEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            9370..9729
                     /locus_tag="OUD09_001387"
     CDS             9370..9729
                     /locus_tag="OUD09_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HCT9692662.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            10253..12334
                     /gene="cadA"
                     /locus_tag="OUD09_001388"
     CDS             10253..12334
                     /gene="cadA"
                     /locus_tag="OUD09_001388"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HCT9692663.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
BASE COUNT         4192 a         2178 c         2694 g         3473 t
ORIGIN      
        1 ttgtgacgtg gtcacgacct ctttttgacg attatgatac aaaaaacatc atttatgaac
       61 aaaatgtgaa taacgggcgt tcttgagata tgattttaat tgggaaaaac ttatcgataa
      121 ggaggtcttt atgaaaaaaa gactgttatg gtgtttgctg tttctatccc taagtttaac
      181 aagtcagcca gtttttgctg aagaaacaca gcggtctcct attacaggca cacgtctgta
      241 caaaaatgtt tatgtacaga caacagatct tgattctgcg attcctgaaa cagaagaacg
      301 gtctttgcag ccaagatttg gttacttttg gtactgtccg acctgtgatt atgaaagttg
      361 ctggcatttc ttacattcta cagctgcagc ttttgctttt actcatactc agaaatatga
      421 tggacatcat cctgttattt atgaggtata gatatgagca aaaaagaaaa actaatctcg
      481 ttgatcgtat tggtttgcct tgttcttctg gtaactgtca taggctatca gaaataccat
      541 gagaacatca gacctactaa agaatcttcc ccagcctcga ccgttgaaac agaaccgata
      601 agtagtcaga aagaataatt tttttagttt aaagaagaac atcagagcaa aaaaacacca
      661 actgaacaat atgtcgttta ttttaaaaaa agttcggttt ttcactttat gtaatcgctg
      721 agaaaacttt ttttaataaa aaaagtcgaa aaactctagc ttttttgtaa acggtatgat
      781 atactatagg agtagttttt gttcggtaat ctgtggtagc ttgtttcaaa taaacatctt
      841 ccagatatac accattcaag taattgcaaa atatgtgttg atacacaagg aggattttta
      901 ttatggaaac aggtacagta aaatggttta actcagaaaa aggatttgga ttcatcactg
      961 cagaaaacgg aaacgatgta ttcgttcatt tctcagctat ccaaggcgac ggattcaaaa
     1021 ctttagaaga aggccaagca gtaactttcg atgttgaaga aggccaacgc ggacctcaag
     1081 caacaaacgt tactaaagct taattaacct atactaaaaa ataaacgccc agacatttgc
     1141 gtgtctgggc gtttttgtct attttcttta aaaagcgatg agagtatttt tctgcacttt
     1201 gtagtataat ttcaaaagaa cattagaggc aggagaagag gaagatattt tgaaaccaag
     1261 aacttatgca gtcgttgata tcgaaacgac tgggacgaat ccaaaagaag atcggatcat
     1321 ccaatttggc tgcgtcatga ttgaatcagg tcggatcact tcacgttttt cgatcgatat
     1381 tcatccaggg cggaagattt caaaacaaat ccagcattta acagggatca cgaatcaacg
     1441 cgtccaaaaa gcgccttatt tcgaagatgt cgcccaaacg atttacaatc ttttggcaga
     1501 caccatattt gtcgcgcata atatttattt tgattataat tttttgaacc atgaactcat
     1561 gcgatgcgga cttcctagtt tgaagatacc aggcatcgat acagtagaac ttgcacaaat
     1621 tttcttgccg acggaaccaa gctttcgctt agctgatttg tcagaaagcc ttggatttat
     1681 tcatgagaat cctcatcagg cagatagtga tgccgaagtg acgggtcaac ttcttttgct
     1741 gatcgaagaa cggatgagaa aattgccaat catcacgtta gagcagattg cgcgattaag
     1801 tagacacaca ggtatggata cgagtcgttt catttatcat gtgattgaag aaatgaaaga
     1861 aaaaccagag ccacttgatc cctcattaga aatcgtcgat gggctagctt tgcagaaaaa
     1921 agaagtggaa ctattcacat ccgtccatta tggcgagcgg acatatccaa gaaaaaaaca
     1981 agcaaaagaa aaaatgtttg gtaaaacact gatgtatcga aaagaacaga acagactgat
     2041 gaatgcggtc tacgaccatt tcacaaaaga tgaatcaaaa gatttgatga tcgaagcagc
     2101 tacaggaatg ggtaaaacaa tcggttatct gctgccattg agttatttgg ctacacctga
     2161 aaaacccgct gtcatcagta ccgtttcgct agttttgcag cagcagatca tagaaaaaga
     2221 cattccattg ctgaatcaat tgctagatca gcccatacaa cctgtaatta tcaaaagcta
     2281 tcgccactat attgatttac agcgttttaa aggcacactt gtggaaccgc cagaacaaaa
     2341 acaatatgct ttgtatcaaa tggcggtctt agtttggctg acgcaaacga agaccggtga
     2401 tttggatgaa ttacatttaa caaatttgaa ccattcattc tttgcagata tcgcgcacag
     2461 aggtattgga tttttagcaa gaaatcagtc attttacgaa caagactttg ttcgtttctt
     2521 gcaaaagaaa atcaggcaaa gtaatttttt gattgtcaat catgcgtttt taatacaaga
     2581 aacacagcga aaagaaccat tgcttccagc tagtccattt ttgttgatcg atgaagtaca
     2641 tcatttgccg gaaatcgctg aaaaagtaaa tgacaaacga ttaagtatta actatttcca
     2701 taaacaaatc cagcaattag aagaaccaaa tcagctgttt gaccgaatac aaagctattt
     2761 accgaaagaa catgaagcct tacgctggtt atcgatttat cgagaagaac tttctgcgct
     2821 atgcgaagca cagatttgta tggcagaagg attaatgagt ttagtgagcg gcaagcaacc
     2881 taacggacag tatccagaag aagtcatgat gacaaaagag atgttggatc agctgccact
     2941 ggaatctggg acagccattg atcacctgca gcttttttat caggagatcc agcagctaca
     3001 gtcacaaatg cgtctgactt ttgagattat ccaagaaagc tggacaaacc agatgcggat
     3061 cgatttagca agtttatata gcctatttga ccaattagac aagcagtact tgctgatgga
     3121 tcaatggctg gaagactggc gagatcattt gatccattgg ttcgttcctg ggaacacagc
     3181 gcagcagatg atttttcatc tccatgattt tcaagcggct atgttgccgt cgactgtttg
     3241 gtatcctcga tatgatcgta ttctttatat tggaggaacg ctgaagatcg gccctaatcg
     3301 acactatctc tctaagaggc ttggcattga agcagcacct cttaaagtga ttgcctctcc
     3361 ttatgattat gagaaacagg caaggatcta tgtgccaaaa gaggcacctg ccgtccatca
     3421 agtcagcaat caagcatata tcgattattt agagacagca cttacaggtc tgatcgaaca
     3481 agaagatcgg ccaatacttg tgttattcac ttctcatgat gttttgcaga aggtctatca
     3541 gaaaatgcat ctgtctatgc tagaaaaagg acgagaaata ctagctcaag ggtttggcgg
     3601 aagccgggag aagcttttga aacgcttcat tttgtcagaa aaaagcgtat tgtttggcgc
     3661 tgacagcttt tgggaaggtg tagatttgcc tggagatgct ttgcagatcg ttgtagtcac
     3721 acgtttaccc ttcgataatc cgcttcgccc gatggtcaaa gctaggaacg cttatttaga
     3781 atcagagggg atcaatcctt tttaccagga atccgtacct aaagcagctt tgaagttacg
     3841 ccaagcatta ggtcgcttga taaggacgga gacagatcgt ggcgtattgc ttattttgga
     3901 tcgaagacta gtcactgcaa aatatggtgc acggatcatc catgcattac cgaaaaaagt
     3961 accgatcaaa gagctagcta tggaggaaat ctatcaggat atccatgaat tcttaaagaa
     4021 aaatgaagaa ggaagttaga ggagctgtct tttttcataa ttaaaatctg ctataatgaa
     4081 aaggaatgaa gataaaggag cgacgaccct tgaagaaaaa acagacaaag gaaaaaacga
     4141 ttaatcgcgt gttacttggc ttgatcgtat ttttattagc ggtcatcgtg atcagctcga
     4201 ttttttactt gcgttccagt cgtccggcgg tgcaggcgaa gaaagaagca gtagaaatcg
     4261 caaagaaata tgcaaatctt gaggaagccg atcattttta ttggtttact cgaaagaaga
     4321 catacttttc actgacaggt acaaatgata aaggaaaaga aatcgccgtt atcattccta
     4381 aatctggtga aaaagtaaaa gtattggatc aaacaaaggg actgactgaa aatgaagcaa
     4441 aacagacaat tgctgcggcc catcctgaaa tccaaatcga aaaagctgct ttagggatgt
     4501 atgatgatca gcctgtgtgg gaagtagtag gaaaagaaac aaatgggaaa ctgaattatt
     4561 acctgattgg ctttgaatct ggagatgaag tcaagacaat caagggaatt taaaaggtaa
     4621 aggaatgaat gggatggagt tatcaaaaag aacaaaacga ttagaaccgt ctgtcacttt
     4681 ggcagctgcg gcaaaggcca aggcattaaa agccaaaggc agagatgtgc tgagtttgac
     4741 tgtgggagaa ccggattttg cgacaccaga aaacatccaa gaagcagcaa tcgaagcaat
     4801 ccgcaatggg aaagctagct attatacacc aacagcagga atcccagagc ttcgacaggc
     4861 aatcgttgat tatctgaaga aaaactatgg acttgtctat gaaccttcac aaacagtcgt
     4921 aacagacgga gcgaaatttg ctttgtatac ccttttccaa acgatccttg acccgcaaga
     4981 tgaagtcatc attcctgtgc cttactgggt cagttacggt gagcaagtaa aactggcaga
     5041 aggacttcct gtctttgtga aaggggaaga atccaatagc tggaaagtta ctgttgagca
     5101 gctagaacaa gcacgttcag aaaaaacaaa agccgtaatc atcaattctc catcgaatcc
     5161 gactggaatg atttatagca agaatgaact gcaggcaatc ggtgaatggg cagtaaaaaa
     5221 tgatattttg atagttgctg atgatattta tggccgttta gtttataatg gcaatgagtt
     5281 tactccaatt gcaacgattt cagaagcaat caaaaatcag accatcatca tcaacggcgt
     5341 gtctaaaaca tatgcgatga caggctggcg cattggttat gcggtgggaa atcctgtgat
     5401 catcaatgga atgatcgctg ttgcttccca atcaactagc aatccgacag cagtcagtca
     5461 atatgcagct gttgaagcat tgaaaggcga gcaagataca gtggaagaaa tgcggaacgc
     5521 ctttgaagaa cgcctgaata cgttatatcc gcttgtggca gaactgccgg gtgtttctct
     5581 tgaaaagcca caaggagctt tttatctttt tcctaatgta aaagaaacgc tacgcatgtg
     5641 taaatatgaa aatgtcacac aatgggtaga agatctttta gaagaaacag gggtagcgct
     5701 tgtgactggc gaaggctttg gggcaccaga aaatgtccgc atgagttatg ccactgatcg
     5761 cgctacgtta gaagaagcgg tacgcagaat caagcaattt attgagagta aaagtcaaaa
     5821 ttaatcctga agaaattcaa ggaatataaa aggagagtca tacgtggaac aaattcaaat
     5881 catcgattcg aaaaaccatg tgggagaaac agtaacaatc ggcgcttggg tcgcaaataa
     5941 gcgatcaagc ggaaaaatcg catttttaca attaagagac ggaacagcct ttttccaagg
     6001 tgtagttgta aaaagcgaag tgccggaaga agttttccaa acagctaaaa atctgcacca
     6061 agaaacatcc gtgttagtaa ctggagaaat ccgtgaagac agccgttcaa aatttggtta
     6121 tgaaatcgga attacaaatg tggaagtggt aagcgagagc cacgattatc cgatcacacc
     6181 aaaagaacac ggaacagact tcttgatgga tcatcgtcat ttatggctac gttcttcaag
     6241 acaacatgcg attatgcaga tcagaaatga aatcattcgt gcaacgtatg aattctttaa
     6301 tcaacatcat tttgtcaaaa tcgatccgcc gatcttgact ggttccgcac cagaaggcac
     6361 tacggatcta ttcgaaacaa attactttga tcaaaaagct tatttatctc aaagtggcca
     6421 attatacatg gaagctgctg ctatggcttt cggaaaagtc ttttcattcg gtccgacttt
     6481 ccgtgctgaa aaatcaaaaa cacgacgtca tttgattgaa ttctggatga tcgaaccaga
     6541 aatggctttc actcatcaag aagaaagttt agaagtacag gaacaatatg tagcattctt
     6601 agtacaaaat gttttagatc attgcgaata cgcattagat gtactaggac gagacaaaga
     6661 agtcttaaaa acttatacaa aattgccatt ccctcgtatt acttatgatg atgcgatcga
     6721 attactgaag aaaaatggat ttgatgacat cgaatggggc gaagactttg gttcacctca
     6781 tgaaacgttc attgcgaatt cattcgaaca gcctgtcttt atcctaaact atccaaaagc
     6841 tatcaagcca ttctacatga aaccacatcc aacaagagaa gacgtggtaa tttgtgcaga
     6901 tatgatcgca ccagaaggtt acggtgaaat tattggcgga agcgaacggg caacggacta
     6961 tgatttcttg ctagaacaga tccgtaatca cggtttggat gaaaaagaat attcatggta
     7021 tcttgatcta cgtaaatacg gttctgttcc gcactctggt tttggtctag gccttgaacg
     7081 aacagtcact tggattgctg gtatcgacca tgtgcgtgaa gctattccat tcccacgttt
     7141 gttgaaccga atttatccat aagaggtgat gaaaaagctg ttctgcatca agtaattaga
     7201 acagccaagc aaaaacagct gttcatgttt ttgaattggc gggacttatt accgcagatg
     7261 taagcttttg aataccgttt agtggagagg ttgtgaaaaa gctgtttagt ttcgagtatt
     7321 aagaaaaatt ttgtggaaat ggctttccac atttttgcat aaatttggct taataccgag
     7381 aaactggctt ttgaacaccg tttagggaag aggttgtgaa atcagtgcct tgacctgagt
     7441 attagagaaa caaaacggaa aaatagcttc tcatatttta ccgttttttt agctaatatc
     7501 gagggtcagc tgattgaaca ccgtttatat caagaggttg tgcgcctgcc gttcagctcc
     7561 gagtcacaag gagtaatttc aaaaaacagt ttttcatgtt tgatgactat tgtgacttgt
     7621 ggccggagag caacgtttat ttaaaaaaaa gaactgcgaa ctctcgcagt tcttttttta
     7681 aaatacggga taatggaagt ttccagcaga caagagaaga aagcaaaaga aattgctatt
     7741 tgattcacaa ggttctataa cgaaatcgca atcaatcaaa catttacaaa gaaggtaatt
     7801 caaatggaaa tctgatttga tgatgcttct gctgttgaaa taaagcagca tctagcacct
     7861 gaaaacaaac taatagtaac attcgaagat ggagtaggtc cgtattttca gcatgcaata
     7921 atccatatgt aagttcaatt cacccttgaa atcatcggac cagataaagt tatgaccaat
     7981 ctatcccaag caatctagaa gagatattag taaagggaca tttactagaa gtcttagatg
     8041 agcctataaa tatatgtttt gatggcgtca tcagtggtct gattgacgga agcttgggta
     8101 tgatcgatct ctctggacca aacaacgaag gaatcaaggg gaatccggca cgttaagaaa
     8161 aaaatagcat aaaaaactag acacttgatg agcgttcatt caattgtttg aagggactag
     8221 gctggtgtca ttttttatac atatattttc tctttcatct tgatagggct tatgggataa
     8281 tataaggaag aaaatcatcg ttttattcaa aaaggagttt ttgacaaatg aaaaaacgta
     8341 tattatttat cggtgatagc attacagacg caggaagaga ccgagcagaa gcaactgatc
     8401 taggtaaagg ttatccactt ttgattgccg gaaaactaat ctcccaatat ccaacgatgg
     8461 aatttgaatg cgtgaaccgt gggataggag gagacaaaat tgcagatctg caagtacgct
     8521 ggcaagaaga ttgtttagct ttacaacctg actatgtatc gattttgatc ggaatcaatg
     8581 atacctggca taatactagc gatcaggcta tattcggaac tgagaaatct gctcagcaat
     8641 ttgaatccgt ttatcgaaaa ttactaaatg aacttcaaaa agcgggaatc tgcaacatca
     8701 tcttgatgga gccgtttgta cttcctataa ccgacgaccg tcatggctgg cgtaaagact
     8761 tagaccctaa aatccaaatt gttcgtaaac ttgctaagga atatggcgca ttgctggttc
     8821 ctttagatgg tatattcaat gctttaggga ttgcttatgg tttttcgaaa tatacaagag
     8881 aagatggggt ccacccaacg attggcggac atgagatcat tgcacaaagc tggatagatg
     8941 caattgccgc agtcgatttt tttagtggag ataaaaatga gcgaaagaaa agtgaaaaat
     9001 gatggaaata aggtgggcag taaaagaaga tttacctcaa atcaatcagc tgattactga
     9061 tcatccagaa tcattcgtaa aagcaaaaac ggcagctgac ctaaatgata agaataaact
     9121 attgctcatt gctgtaacaa aagaaaaaca gattatcgga tttgcgcaag cggacgtgca
     9181 acagtcagaa gaacgattgg atcagcgacc atcaggaaaa ctaatccaac acacattagt
     9241 aacaaaagaa aatacgaaag aaatccaaaa aaaatttgca gaatacttga tgctggaagc
     9301 taaaaaaaga agaataaaat caatttttgc ccagttttaa tcaaaaacag taatagaagg
     9361 agaaatacaa tgagttgttt aggaaatgtc atctggttta tattcggcgg actaatcggt
     9421 ggtcttagct ggttagcagc ggggatactt tggtgtatca cgatcatagg tatcccaatc
     9481 ggtatgcaat gttttaagat cgcctccctt tcttttgcgc cttttggcaa agaagtaatt
     9541 tatcaccagt ctggcatgtc attgatcgtt aatatcatct ggctgatcgt ttcaggccta
     9601 ccaattgctc tcgcacatgt cgtcagcggc atccttctat gtatcacgat catagggata
     9661 ccttttgcca agcaatcttt caaacttgct cgattggcgt tgatgccatt cggtgcagaa
     9721 attgtataaa agagaataat aaaggttaga taacgcatag agtcaggtgg gatacattta
     9781 tttcacctga ctctatgcgt tttcctgtat aagttcttcc atgtgacttt tacgtctttt
     9841 atttatatgg gattactgtt tatggaaagt tgaaataatt ttcattaaag aactctttct
     9901 atatttttga aagcgtagtg gagcttcgaa tatctgggaa ggacattttg cagggattga
     9961 aaaaagatat tgtcctatcg aaaaattaat gaatttagac aaattgccag caacaggctt
    10021 caaacttagt tgctatcctg tcaagatcaa gaaggcaagt gcgggttgga taagagcagt
    10081 ggcaatgatc gaaaagaaaa agaaagaata agccaaaaca tctgtatgga agaaattttc
    10141 cgatgcagat gtttttcatt ttttctttcg gataccttga catatgaata accattcatt
    10201 tataattagt tatgaagaga tattcatatg ttgaggcgag gaggtcatta aaatgaaaag
    10261 ttacagatta gaaggtttag attgtgcgaa ctgtgcaatg aaaatcgaaa aaggagttca
    10321 gaaaatcaat ggagtcaagg aagcaacggt aaacttcact tctggaaaac taacgattga
    10381 cgcagaggaa gatcatttgg ctacgattga acaagaaaca aaaaaagtag tgaaggaatt
    10441 agaaccagat gtcaaagtaa cagaaataga taaggaaaaa gtatctgaac atggaaatga
    10501 aaaagagaga aatacgctgt tccggatttt attttccctt gcaggtatcg ctttattgct
    10561 attatttgat tttaatgaac caattcgctt gatagggtat ttattgattt atctgctgat
    10621 aggatatgat gtagtgaaga aggcagtgat gaatatcgtc aaaggaaaaa tctttgatga
    10681 gaattttctt atgtctgttg ctacgattgg cgctatgttg atcggtgaat atccagaagc
    10741 cgtagcagtg atgctatttt atcaaatcgg cgagtatttc caaggattgg cagtcagtca
    10801 ttcaagaaaa tcgattcgcg aattgatggc gatccgtcca gaaacagctc acgtgcagac
    10861 ggcggaagga ttgatgaccg tgaaccctga agacgtgcag atcggtcagt ttgtacttgt
    10921 gaagcctggt gaacgtgtac cgttagatgg aacgatcatc gaaggagaat cattagtcga
    10981 tacgtcagca cttacaggag aatcagtccc taaatctgta tatgttggtg aaacagtatt
    11041 aagcggattt atcaataaaa ataaaccatt tttagttcaa gtagaaaaat cttatgaaaa
    11101 ttctactatc agcaaattat tagaattagt agaaaatgcc agcagtaaaa aagcaccagc
    11161 tgaaaatttc attactaaat tcgcgcgcta ttacacacca gtcgttgtag gattggccgt
    11221 tttattagct gttttacctc cacttgttgt ttctggagcg gcgttttctg aatggatcta
    11281 tcgagcattg acatttttag tgatctcatg tccatgtgca ttagtgattt ccgtgccact
    11341 tagtttcttt ggcggtatcg gaggagcaag taaaatcggt gtattagtaa aaggttcaaa
    11401 ttacttggag ttgcttgcac aaactgagac agtggtattc gataaaaccg gcacattaac
    11461 gaaaggtgac ttttcgatcc agacgatcga tacaaaaatc gatccaaaga tattcatgca
    11521 atatgttgcg agtgcagaac agttttctac acatccgatt gctcaatctg ttttagaagg
    11581 ctacgcaggt ccgcttttac caacaactaa tatccaagaa tttgcaggtg aagggatctt
    11641 agctgaagtt gatggcaaac aagtattagt aggtaatcac aaattgatgg aaagatttga
    11701 aatctctttt ccttcaagtc aagaaatcgg tacattgctt tatctagcga tcgatcaaag
    11761 ttacagtggt tatcttgtta ttgcagatac cttgaaagaa gatgccgtag acgcattggt
    11821 tcaattaaaa caggctggtg tgaagaacac agtcatgttg acgggcgact cgaaaaaaat
    11881 tgctgatcat atcgggaagc aagtaggcgt ggacaagata tatagcgaat tgcttccaga
    11941 agataaagtg cagcggttag aggaaatctt acaaagaaac aataaaaaga ccgcatttgt
    12001 cggagatgga atcaacgatg caccggtgct agcaagagca gatgtaggaa tcgcaatggg
    12061 tggattggga agcgatgctg caattgaagc agcagatgta gtcatcatga atgatcaacc
    12121 atcgaagatt gcagaagcaa tccatttagc aaagaaaaca ttgaaaatcg tcaaacagaa
    12181 cattgtcttt gctattggga taaaaatttt ggtacttgcc ttaggtgctt ttggttttgc
    12241 atcaatggga gatgcagtat ttgcagatgt tggggtgaca gtattagctg ttttgaatgc
    12301 catgcgaagc ttgcatgtaa aaaataaacg ttgatcaatg aagaggacga atgaacgtcc
    12361 tctttttttt atatcctttt cttaatgaaa aatgtaaaga aaatagataa gattataaaa
    12421 taactaatgt ttttaatttt gcagaaaatt caaaatagct tgaaattcaa taaggtcaat
    12481 gaaataaaag aaaataactt atttttttga tggaaaaaat atgaaaaaac gatgtgt
//
LOCUS       DAKBVG010000072        12528 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000072 DAKBVG010000000
VERSION     DAKBVG010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.072"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            12..857
                     /locus_tag="OUD09_001389"
     CDS             12..857
                     /locus_tag="OUD09_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCT9692664.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YPLPQLMKHLIKRFPKVHFEIEIANSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            1048..2235
                     /locus_tag="OUD09_001390"
     CDS             1048..2235
                     /locus_tag="OUD09_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HCT9692665.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYFYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVVGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            2241..2597
                     /locus_tag="OUD09_001391"
     CDS             2241..2597
                     /locus_tag="OUD09_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HCT9692666.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            2599..2937
                     /locus_tag="OUD09_001392"
     CDS             2599..2937
                     /locus_tag="OUD09_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HCT9692667.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    3013..3269
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3332..4366
                     /locus_tag="OUD09_001393"
     CDS             3332..4366
                     /locus_tag="OUD09_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCT9692668.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            4359..5045
                     /locus_tag="OUD09_001394"
     CDS             4359..5045
                     /locus_tag="OUD09_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT9692669.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            5069..5917
                     /locus_tag="OUD09_001395"
     CDS             5069..5917
                     /locus_tag="OUD09_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HCT9692670.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            6114..6887
                     /gene="sufC"
                     /locus_tag="OUD09_001396"
     CDS             6114..6887
                     /gene="sufC"
                     /locus_tag="OUD09_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCT9692671.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            6900..8186
                     /gene="sufD"
                     /locus_tag="OUD09_001397"
     CDS             6900..8186
                     /gene="sufD"
                     /locus_tag="OUD09_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCT9692672.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            8183..9418
                     /locus_tag="OUD09_001398"
     CDS             8183..9418
                     /locus_tag="OUD09_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCT9692673.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAH
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     SFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVINPVQELAELVHENGGVIVVDGAQSAPHMTIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQELGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VIAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFKHGTI"
     gene            9405..9875
                     /locus_tag="OUD09_001399"
     CDS             9405..9875
                     /locus_tag="OUD09_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HCT9692674.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            9880..11271
                     /gene="sufB"
                     /locus_tag="OUD09_001400"
     CDS             9880..11271
                     /gene="sufB"
                     /locus_tag="OUD09_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCT9692675.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(11369..12511)
                     /locus_tag="OUD09_001401"
     CDS             complement(11369..12511)
                     /locus_tag="OUD09_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCT9692676.1"
                     /translation="MATFFSYKLQNGEKYWGFETYLGKDPKTGKDIRTKRRRFKSKAE
                     AQTALNRLLVDFKEKQEFKINVTTFQELYELWIDNYRTRVKPSSVAVAQRFCKNHILP
                     AFGELRLDKISVSYCQKQVNEWHKTLKQYGFLRKTTAQIFKFGVAMEVCKDNPMSKTL
                     LPRKIEEEQPLKFYTKDQLQLFLQNTKENNSVKMYTFFRLLAYTGMRKSEALALQWED
                     IDYFNKTITIGKTIAQDEFNQVVLQVPKTKNSSRTIQLDDFTLKQLRIWQQEQMKIMI
                     LYGYNTNSPKQFLFTTNTNKLYYPQVVNDWLDWIYKKTPMEPQITPHGFRHTHCSLLF
                     ESGASIKEVQERLGHKDIKTTMNIYAHVTPQSVKKTGDRFAKFMGE"
BASE COUNT         4009 a         2351 c         2747 g         3421 t
ORIGIN      
        1 gaagtggtgc catgtttaag ttattaacga cttttcgagc agtatatgaa acaagaaatt
       61 tttccagagc agcagaagta ctgtttttat ctcaaccagc agtctcgaat caaatcaaac
      121 agttggagcg ggaattgaat atcgatcttt ttcaacgtaa cggaagaaaa gagatgcttc
      181 caactaagca agccgactta ttatatgaac ggacactggt tttgttagaa gaatgggaag
      241 aaactcagca aaaattgctc aatgaacaaa atgaagaaga agtatgtcga atcgttgctt
      301 cccacacctt tgctgtctat cctttgcctc agttgatgaa acatctgatc aaaaggtttc
      361 ctaaagtgca ttttgagatc gaaatcgcga attctcatga atcattagaa caagtagcga
      421 aacatgaatc cgattttggt tttatcgaaa agccattaga gacttccggc gtacaaagat
      481 actctttgat ggaagaccag ctagtcatcg ctggggaccc aaaaagcaaa ttgtggcttg
      541 taagagaagc aaactctggt gtttttcatt acacgcagcg ttattttgaa gagatgaata
      601 tccaagataa gaagatgatc gtgaaaagta acgatgtaat tgttgcgttg ctgaaggaag
      661 gaatcggtcg ctcgattttg tctaagcgct ctgttccaga aaatgtgcca atgactccgt
      721 tgaatttcca tcgttatttt tatttcatcc aacgaaccaa tctggtttct gatcagctgc
      781 tagagattgc cgaagagatc cttcgataca atgaaagcac agaaacttct cacagcgagg
      841 agaatctcag ttggtagata attgcatgta tcaaaaaaag ttctatgata cactaaagat
      901 aagcagtagg cttttgtact gcatttgctc aagcaactgc acaaacggaa agttaatttt
      961 tcccaaatgt ctatggaaag actttccatt atctaactgg accgctataa taatcagcag
     1021 tatggttttt aagaaaaggg atgaaacatg gaaaaaaaat taaaagcttc gaacgataat
     1081 cgtattgatt atggcgtgat tttaccagtc tttctgttat gtttgattgg tctgctctca
     1141 ttatatgtag cacttagcca tgatccgaac aatccagcag tcttgaaagg cgtagggatg
     1201 caagttgtct ggtatttagt tggagcggca gcaatcgttg tcatcatgca tttgaattcc
     1261 aaatggatct ggaaactgac tccttacttt tatggtttgg ggttagtggt aatgggactc
     1321 ttgctcaaat tctatgatca gaatttagct gcttcaacag ggtctaaaaa ctggtttcga
     1381 ttcggtgcgt ttactttcca gccagcagag ttgatgaaga ttgcgtacat cttgatgatg
     1441 gcactgatcg taactaaaca taatacgcaa gtaaaagaac ggacgatgaa atcggacttt
     1501 tggctgattg ggaagctctt gatcgtgaca ataccggttt tagcattgat catggcccaa
     1561 gatgactttg gaacgatgct tgtctttctt gcgatctttg gcgggatttt cttgatgtct
     1621 ggaatctctt ggcgcatcat cgcacctgta gtaatcttgg cagtagtcgt tggagcagga
     1681 acgatcttct tggttacgac tgaaggcgga agagacttgc tttacaaagt tggcttcaaa
     1741 tcttatcagt ttgcacgtat tgattcatgg ctggaccctt tccatgatac gtcaggaatg
     1801 agttatcagc cggcccaagg acttttggca attggtaccg gtggcttgtt tggtaaagga
     1861 ttcaacgtaa gcaatattta tgtgcctgtt cgtgagtccg atatgatttt taccgttgtc
     1921 ggtgaaaact tcggctttat cggtggtgca tttgtcattt tcttgtattt tattttaatc
     1981 tatcgtatga ttcgggtgtg tttcgatacg aataatgaat tttacgccta tatcgcaagc
     2041 ggtttgatca tgatgctgct gttccatgtc tttgagaata ttggagcaaa tatcggatta
     2101 cttcctttaa cagggatccc attgcctttc attagtcagg gaggatcttc gattttaggg
     2161 aacatgatcg gtatcgggtt gatattatcg atgagatacc aaaatgaagc acctgcaaga
     2221 tcttcaagga ggtaagaaaa ttgtacacct tttactggta cccgaaatgt tcaacatgca
     2281 aaaaagccaa agcgtggtta gaccagcatg gcgttagtta tcaaactgtt gatatgatca
     2341 aagaagtacc gaaggcctca acgattgaga cttggctaga aacgagtcaa gtaccaatcc
     2401 gtcgcctgtt caacacaagc gggatgaaat atcgggaact tggactgaaa gatcaagtag
     2461 gcgagtttac ggcttcaaaa gcgagccaag tcttatcgac agatggtatg ctgatcaagc
     2521 ggccactgat cttacgagag gaccagcttg ttgcaattgg attcaatgaa aaaatttatg
     2581 aaggagaact gatataaaat ggcaggcaag catttgaaga aaaaggacaa cctttggatc
     2641 ttatttaatg gaaaagaata ctgtgttggt ttgacaaatg aagcgcaaga agaacttgga
     2701 gacatcacgt ttgccaatct gccaaaagca ggacaatcgc taaaaaaaga tgaacctctg
     2761 atcgaagttg aagcggaaaa agcggtcaat gaatttgcca gcccgctaac gggtacagtt
     2821 tcttctgtta atgataaaat cggtgaagat atcaatatct tgaatgattc agatgaaatg
     2881 aatgcgtgga tcttaagttt caaagatgtt gatccaaaag aatttgacga attataaaat
     2941 cagaaaatca cttgaaaaaa gttgacactg attcttccag ttgctataat cataagcaat
     3001 cctatatgaa acgagaaagc tttgaaaaga agagtacatg tcacggacgt ttgaagagag
     3061 ccctgtttgc tgaaaatggg taacgaaaat gagatggaag atggtcttag agcagagtaa
     3121 gcgagcgtaa gcaaacttac tacgggagtg cccgttacag cgcgacagta tacggaaaga
     3181 agctaagtcc aagtactgga agaggctgtt attgcgaagt aacggtaaat caaggtggta
     3241 acacggaagt tcaagctttc gtccttttgc ttaaggcaag agggcgaagg cttttttgtt
     3301 tgaaaaatca taagagaaag atgtgaatgg catgtcatta atccaactaa cgaatgtcaa
     3361 aaagacattc aatgggaaaa acggaaacat ccaagccgtc aatgacgttt cactaaacgt
     3421 tgaaaaaggg gatatttatg gaatcgtcgg ctattctggc gcaggaaaaa gtacattagt
     3481 ccgtctcctg aatggacttg agcttcccac gagcggcgaa gtgatcgtaa acgatcaaga
     3541 tattacaaaa ttaaaaaaca aagaattacg tacattccga aagaaaatcg gtatgatctt
     3601 ccagcatttc aacctgttat ggtcaagaac tgtacttgaa aatatccagc tgccgctgga
     3661 attagctggc gtaccaaaga gcaaacgtaa agaacgggca gaagaactgt tgcgtttagt
     3721 cgggttagaa ggacgcggac aagcatatcc ttcacaactt tccggcgggc agaaacaacg
     3781 cgtcggtatc gcacgagcat tggcaaatga tcctgagatc ctgctttgtg atgaggctac
     3841 tagcgcattg gacccgcaaa caacagaaga agtattagac ttattgttag cgatcaataa
     3901 gaaattgaat ttgaccatcg tcttgatcac acatgaaatg aatgtgatcc gcaaaatctg
     3961 caacaaagtc gctgtgatgg agttggggaa agtcgtagaa gaaggagacg tattgactgt
     4021 cttcagacaa ccgaaaaaag aagtaaccaa acgctttgta caacaagaca tcgaaccaga
     4081 agctgattcg acagaactat tagctgaact catcaaagaa aatccaagcg gccgtttggc
     4141 aactttgacc tttagcgaaa gaaatgccaa cgagccagtc atttcccaag cgattcgacg
     4201 ctataacatc gatgtcaacg ttgtgtatgg aaagatcaaa caagtgaaag aaggttcctt
     4261 tggttcattg actgtcttga tgactggtga aaccaaagaa ttagatcaag cagaagtgtt
     4321 catcaaagaa caaggagtag gaattgaggt gatccatcgt ggataaaagc ttttcagaaa
     4381 catatctaga ttttaaacaa atcaacccgg aaacgatgaa tcaggctatc cttgatacac
     4441 tatatatgac attaatctca atggtcttag tgacgatcat cggattagtt ttgggattga
     4501 tcctttactc tattggacgc aaaaacaaac cgtatgctcg aatcatttat ggaatcgtat
     4561 cgatcatcag taatattttc cggtcaacgc catttatgat cctgatggtt ttgatcattc
     4621 catttactaa agcaatcgta ggcactatgc taggtgcgca agcagccatt ccagctcttg
     4681 ttttatccgc agcaccattt tatgcccgtc tagtagagat tgcttttcgt gaagtcagcc
     4741 ctggtgtact ggaagcagca gatgcaatgg gagcaagtta ctgggaaatc atctggaagg
     4801 tcttgatacc tgaaagtatt cccgcattga tttctggatt aacagttaca acgatctcga
     4861 tgatcggatt tactgcgatg gccggcgcga tcggcgcagg tggacttgga ggtcttgctt
     4921 ggcaggaagg ctaccagcgt ggtaatctga cagtgacatt cgttgcgact ttaattattt
     4981 tactaatcgt atttatcgtt caaggaatcg gtgatttctt aactaagaaa acagacaaaa
     5041 gataatagga tataggggga tgtaggatat gaaaaagaaa atttttggat ttgcagcagc
     5101 gcttttgctg actgtaggat tagcagcatg cggtaacaac agcgacagca aagattcagc
     5161 aaataaagca gacgacacga cattgaaagt cggcgcttca ccgacaccgc acgcagagat
     5221 tttggaacat gtgaaaccat tattgaaaga tgaaggaatc gatcttgaaa tcgtcaaatt
     5281 cgatgactat gtattaccga accaatcttt ggaagaaggc gacattgatg ccaattactt
     5341 ccagcatatt ccttatttga acaaagaaat caaagaaaaa ggatatgact ttgtcaatgc
     5401 cggtgctgtc cacatcgaac caatgggctt gtactctaaa aaagtcaaag atgtttccga
     5461 attaaaagat ggtgcaacaa tcattacttc aaattctgaa tcagactggg gacgcatcat
     5521 cacaattcta caagatgctg gcttagtcaa agtcaaagat ggagtagatc tggaaacagc
     5581 aacattcgat gatattgaag aaaaccctaa gaacttgaag ttcaaccata cgatcgaccc
     5641 atcactttta gcttctacgt accaaaatga tgaaggtgac ctagttgcaa tcaacgcaaa
     5701 cttcgcttac ggcgcagggt tgaatccatt agaagatgcc gtattgcttg aaaaggacaa
     5761 ctctccttac gtgaatatta ttgcgactcg taaaggtgat gaagacagtg acaaaatcaa
     5821 aaaactgatt gaagtgcttc accgcaaaga agtccaagaa tggatcacag acaaatgggg
     5881 cggctctgtt aaaccagtaa aggctgatgc aaaataaggt atggaacaaa aaagatgaga
     5941 aataagtctg taatggctta tttctcatct tttttatacc tattctaatt tagaaatcaa
     6001 aattgtttaa taataaataa aagatatttg attttagtaa ggaaggtcat tgcgcttcat
     6061 tctcaattag tctaaaatga gtgagaacga aagagaattt gggggtataa tgcatgtctg
     6121 tcttagaaat aaaaaatcta catgtgtcga tcgaggaaaa agaaatcctt aaaggcgtaa
     6181 acctaacaat gaaaaccgga gaaatccatg ccatcatggg acctaacggc acaggaaaat
     6241 ctaccttatc tgccgctatc atgggcaatc caaactatga agtaacagaa ggcgaaattt
     6301 tgtttgacgg gaaaaatgtt ttagacttgg aagtagatga acgtgcgcga cttggcttat
     6361 ttttagctat gcaatatcca agtgagatac caggtatcac gaatgcggaa ttcatgcgtg
     6421 cagcgatcaa tgcgaaacgt gatgaaaacg acaaaatttc tgttatggca tttttgaaaa
     6481 agttagatga aaaaatggct ttactaaata tgccagaaga aatggctgaa cgttatctga
     6541 acgaaggttt ttctggcgga gaaaagaaac ggaatgagat cctgcaattg ttgatgttag
     6601 aaccaacatt tgcaatttta gacgaaatcg attcaggtct ggatatcgat gctttgaaag
     6661 tcgttgctaa aggagtaaat gaaatgcgcg gagacaattt cggtgcattg atcatcaccc
     6721 actatcaacg attattgaac tatatcacac ctgatgtcgt tcacatcatg atggaaggcc
     6781 gagtagtcat gacagggaat gctgacctag ctaaacgtct ggaagcagaa ggctacgctg
     6841 gtatcagtaa agaattagga atcgactata aagaagaaga agcgtaaggg ggagcaagaa
     6901 tgaaagaaaa aaactggctc gattatcttg acgcggtaaa tgatttttct ttaagtaaag
     6961 gcgaacctga ttggatgaga accttccgtc aagacgcact agcgaaagca gatgaactgc
     7021 cattgccgca tatcgatcgg gtgaaattcc atcgctggtc gttatttgat gtgaaagaaa
     7081 cacaaaccat cagtgaaaca ggcacgattc cggcatttga tgcaatgaaa gacaatcctg
     7141 tgcttgtcca acaaggttca tggacgattt ttgaacaact gcctgtggaa ttagcagaaa
     7201 aaggagtcat ttttaccgac ttgttcactg caatgatcga atatccagaa ctagtccaag
     7261 aatattatat gaaaaaagca gtaaacatga acgaagacca attgactgca ctccatgtag
     7321 cttttatgaa cagcggtatt ttcctgtatg tgcctaaaaa tgtcgtgatc gatgaaccac
     7381 tcgagtcctt attcattcaa gatggcgcat ccgatgagca cttcttcaag catgtgttga
     7441 tcgtggcaga tgaacatagt gaattttctt atctggaacg tttccagaca accaaagaac
     7501 aagtagcaaa atcatcaggt aatatcatcg tggaagtcat cgctaaagct ggttcaaaaa
     7561 tcaaatattc agccgtagat caattaggag aaaatattac ttcttatatg aatcgccgtg
     7621 ggcacatttt acgagatgct tctgtcgact gggcaattgg tgtcatgaat gacggtcatg
     7681 tgatcgctga ctttgactca gatttagcag gagaaggtgc acacgcagaa gtcaaaatcg
     7741 tggcaatcag ctcgggcaga caaatccaag gaatcgatac gcgagtaacc aataaagccc
     7801 ctcatacgat cgggcatatt ttgcaacacg gagtgatccg agaaaaagga acattgacct
     7861 ttaatggtat cggccacatc ttgaaagggg caaaaggtgc agatgcccaa caagaaagtc
     7921 gtgtcttgat gctttctgat aaagcacgtg gtgatgcgaa tcctattctt ttgatcgatg
     7981 aaaacgaagt aactgctgga catgctgcca gtgtaggacg agttgatcca gaagaaatgt
     8041 actatctgat gagtcgagga ttgcataaag aagaagcaga gcgcttagtg attcgaggat
     8101 tcctcggttc tgtcttaaca gcaatccctg tagaacaagt tcggaaagaa ttagtcgaag
     8161 taatcgaagg gaagttgaat ggatgatcga tgctaaaaag ctacgcaatg attttcctat
     8221 cttatttcaa gacgtcaatg atgaaccttt agtttatctg gataatgcag cgacgaccca
     8281 aaagccaaaa caagtactcc aggcattagc gcactattac gaacacgata acgccaatgt
     8341 tcatcgcggt gtccatacgt tagcagaacg cgcgacccat gattatgaac aagcaagaga
     8401 aaaagtacgg gcgttcatcc atgcaaaaga aacagcagaa gtgctgttta ctcgtggtac
     8461 gacgacaagc ttgaattggg tagccagaag cttcggcgac cgctatatca aagcaggaga
     8521 cgagatagtg ctctcttaca tggaacatca ttctaatgtt atcccgtggc agcagttggc
     8581 aaaacgaaca ggtgctgtct tgaaatatat cgaactaaca gaagaaggct tcctggatat
     8641 ggaggatgca gcgaaaaaga ttacggacaa aaccaaaatc gtctccattg ctcatgtatc
     8701 caatgtctta ggtgtcatca atcctgttca agaattagct gaacttgtcc atgaaaacgg
     8761 cggggtcatc gtagtagatg gtgctcaatc tgccccgcac atgaccattg atgttcaagc
     8821 actagactgt gatttctttg ctttcagcgg acacaaaatg tgcggaccaa caggtatcgg
     8881 tgttttatat ggtaaacgaa aatggttaga agaaatggaa cctgttgaat ttggtggcga
     8941 aatgatcgat tttgtcgaat tatacgacag tacatggaaa gaactacctt ggaaattcga
     9001 agcagggaca ccaaatatcg ctggcgctat cgcattaggt catgcaatcg attatttgca
     9061 agaactcggc atggacaata tccatcgata tgaagaagaa ctagccgctt atgtgttgcc
     9121 aaaattacaa gcaatcgatg gattgactgt ttatggtccg caagacccga attttcggac
     9181 aggagtcatt gcgttcaatt tggatgggtt acatccccat gatgtcgcga cagctttaga
     9241 catggaaggg gtagccgtta gagcaggtca tcattgtgcg caaccgctat tgaagtacct
     9301 tgaagtatca gcgactgctc gcgccagctt ttatttctac aacacaacgc aagacgccga
     9361 ccgtttagtt gaagcaatca tggcaacaaa ggagtttttc aaacatggga ctatctaaat
     9421 tagacaattt ataccgtcaa gtgatcttgg atcattcttc ccatccacat catcgtggaa
     9481 gcttgactga ctcgacaagc aagatcgaat tgaacaaccc cacatgtgga gatgtgatcg
     9541 aattgcaagt tgatgtaaaa gatggaatca tcaaaaatat cgcctttgac ggcagcggct
     9601 gttccatcag tacagcaagt gcctcgatga tgacagatgc cgtcattgga aaatcaatag
     9661 aagaagcaga acatttaact gtagactttc tacagcttgt ccaaggaaaa gaagtcaaag
     9721 atcccgacca attaggcgat gctgctatgc ttggcggtgt agctaaattt cccgctcgga
     9781 tcaaatgtgc gacacttgcc tggaaagcac taggaaaagc aatggaaaac gatggtaccg
     9841 cagctttcca tcattaccac aaggaagagg agtgagaaca tgggcgtacc agaattagaa
     9901 gaatataaat tcgggtttca cgatgatgtc gaaccggttt tcagtacagg agaaggcttg
     9961 acagaagaag tgatccgtga gatgtcaaga atcaaaggcg aaccagaatg gatgcttgaa
    10021 ttccgtctga aatctctaga aacattcaat aaaatgccaa tgcaaacatg ggggcccgac
    10081 ctttcagaca tcgatttcga tgccatcaaa tattaccaaa aaccaagtga caaacccgct
    10141 cgtgactggg aagacgtacc agaaaaaatc aaagatacat ttgaaaagat tggtatacca
    10201 gaagctgaac gagcttatct agccggtgct tccgcacaat atgaatcaga agtcgtgtac
    10261 cataatatga aagatgaatt cgaaaagtta ggaatcgtct ttactgacac tgactctgct
    10321 ttgaaagaat accctgaatt attcaaagag tacttttcaa aacttgtacc gccaaccgac
    10381 aacaaattag cagcgctgaa ttcagccgta tggtcaggtg gcacctttat ttacgtgcca
    10441 aaaggcgtca aagtcgatgt tccattacaa acgtacttcc ggatcaatgc ggaaaacacg
    10501 gggcaattcg aacgtacgct gatcattgtt gacgaaggag caagcatcca ctatgtcgaa
    10561 ggttgtacgg cacctactta ttcaagtaat agtctgcatg ctgccatcgt agaaatcttt
    10621 acaagaaaag atgcgtattg ccgttacacc acgatccaaa actggtcaga caatgtgtac
    10681 aacttagtaa ccaaacgagc aaaagcatac gaaggcgcaa ccgttgaatg gatcgacgga
    10741 aacttaggtg caaaaacaac gatgaaatac ccaagtgttt acttagatgg aaaaggtgca
    10801 cgaggcacca tgctctccat cgcctttgca ggagcaaacc aaatccaaga tacgggtgca
    10861 aaaatgatcc ataatgcacc aaatacatct agctctatcg tctctaaatc aatttcaaaa
    10921 gacggcggag aagtaaacta tcgaggacaa gtaacctttg ccaaaaacag tgcgggttcg
    10981 atctctcata tcgaatgtga cacgatcatc atggacgaat ggtcgaaatc cgatacgatc
    11041 ccattcaatg agatccacaa cagccaagtc tctttagaac acgaagccaa agtctcaaaa
    11101 atctcagaag aacaactcta ctatctgatg agccgaggcc tgaccgaatc tgaagcaact
    11161 gaaatgatcg tcatgggctt tgttgaacca ttcaccaaag aacttccaat ggaatatgca
    11221 gtcgaactca accgattgat cagttatgag atggaagggt ctgtgggtta acaactattt
    11281 cattgctcga aacgttgata ttaaagcgtt tcgagcgttt ttttatttgt attttaatgg
    11341 ttggctacgt ttttggctac gtttggaatt attcgcccat aaattttgca aatcggtcac
    11401 cagttttctt aacagattgt ggtgtgacat gagcatagat attcatggta gttttaatat
    11461 ctttatgacc taatcgttcc tgtacttctt ttatcgacgc accactttca aacaataagc
    11521 tacagtgggt atgcctaaat ccatgtggag taatttgagg ctccatcggt gtctttttgt
    11581 aaatccagtc taaccaatcg tttactactt gggggtagta caacttgttt gtgtttgtag
    11641 tgaaaagaaa ctgttttggt gaattcgtgt tatagccata taagatcatt attttcattt
    11701 gttcttgttg ccaaatacgc agttgtttaa gtgtaaaatc atcaagctga atcgttcttg
    11761 atgaattttt agttttaggt acttgtaaaa ctacttgatt aaactcgtct tgagcgatag
    11821 ttttaccaat tgttattgtt ttgttgaaat agtctatatc ttcccattga agcgccaatg
    11881 cctcgctttt acgcattcct gtataagcta ataaacggaa aaaagtgtac attttaactg
    11941 aattattttc tttagtattc tgtaaaaaca gttgtaattg atctttcgtg taaaacttca
    12001 agggttgttc ttcttcaatt tttcttggaa gcaatgtttt agacatagga ttatccttac
    12061 agacttccat tgctactcca aatttaaaaa tttgagcagt tgtttttctc aagaaaccat
    12121 attgttttaa tgttttgtgc cattcattca cctgtttttg gcaatagcta acacttattt
    12181 tatccaatcg taattcccca aaagcaggta atatatggtt tttgcaaaaa cgttgagcaa
    12241 cggcaacaga agacggtttt actcttgttc tgtagttatc aatccataat tcatacaact
    12301 cttgaaaagt tgtaacattg attttaaact cttgtttttc tttaaagtct acaagtagtc
    12361 tattaagtgc tgtttgcgcc tcagcttttg atttgaatct tctacgcttt gtacgtatat
    12421 cttttccagt cttgggatct ttccctaaat aagtttcaaa tccccaatat ttttcgccat
    12481 tttgtaattt ataactaaaa aatgttgcca tttgattctc tccttact
//
LOCUS       DAKBVG010000073        12180 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000073 DAKBVG010000000
VERSION     DAKBVG010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12180
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.073"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(142..306)
                     /locus_tag="OUD09_001402"
     CDS             complement(142..306)
                     /locus_tag="OUD09_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9692677.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            601..1929
                     /locus_tag="OUD09_001403"
     CDS             601..1929
                     /locus_tag="OUD09_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HCT9692678.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2131..2964
                     /locus_tag="OUD09_001404"
     CDS             2131..2964
                     /locus_tag="OUD09_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCT9692679.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2980..3717
                     /locus_tag="OUD09_001405"
     CDS             2980..3717
                     /locus_tag="OUD09_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HCT9692680.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3872..4840)
                     /locus_tag="OUD09_001406"
     CDS             complement(3872..4840)
                     /locus_tag="OUD09_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCT9692681.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELKKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5072..5905
                     /locus_tag="OUD09_001407"
     CDS             5072..5905
                     /locus_tag="OUD09_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCT9692682.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5939..6778
                     /locus_tag="OUD09_001408"
     CDS             5939..6778
                     /locus_tag="OUD09_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCT9692683.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6804..7364
                     /locus_tag="OUD09_001409"
     CDS             6804..7364
                     /locus_tag="OUD09_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCT9692684.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7563..9284
                     /locus_tag="OUD09_001410"
     CDS             7563..9284
                     /locus_tag="OUD09_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HCT9692685.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9449..10591
                     /locus_tag="OUD09_001411"
     CDS             9449..10591
                     /locus_tag="OUD09_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCT9692686.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10607..11818
                     /gene="thiI"
                     /locus_tag="OUD09_001412"
     CDS             10607..11818
                     /gene="thiI"
                     /locus_tag="OUD09_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCT9692687.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4152 a         2041 c         2530 g         3457 t
ORIGIN      
        1 ggagtaaata aagcttatgc aaagaaaaag aaatccaatc acttgggttt ctttttcttt
       61 tattattatg atgaatcaag ctttctgttt ttgtcacgta tgttgtaatc tttacgaaag
      121 agtagagttg gctactcata atcaaccata cttcccatca tcgctaaata cgaaataaat
      181 cagcattcct acaccaatca aagcacaagc tgcaccaaga aaagggatga cattcccaaa
      241 gttatctgta ttcataagta tgccaccacc cacaaacaaa aagccgccag caatcaggtt
      301 tttcatttta ttcatctcct ttcttacgtt atagtatttc cttctttcaa tgagtatttt
      361 gaaagctatc ttattgtatg atgttcttct aggcctcttc tttattatac aggccatttt
      421 cgttatctca tagaaatatt cctttttatc tgaaaactat tgtataccgt atgacatttt
      481 tcttcatgaa gttgtaagta tattctcatt caaatgaaaa tttcatcata gaagagattg
      541 tatttctaaa ttcttaagag taaaatacga agtataactt ggatttggaa ggatgattgg
      601 atgaaagcta ttacgcctga tatgacaaca aaatttaaca aacagtttca agcaaatatc
      661 aaacagcagg cattgcagcg ggcagttgtg aagaacggaa ttactgcttc tgctgaaaat
      721 gtttcagcac atgtggagaa tacaccggta ttttctatag atttggctac ggggaaagtg
      781 gctaatcaaa tgcaatcggg gcgttgctgg atgtttgctg cactgaacac attccgtcat
      841 aagatgctga atcaattcca tttaaaagag tttgaattat cacaaaatta cacttttttc
      901 tgggataaat atgaaaaggc aaattatttc tatgaaaaca tcattgcaac aggaaaagag
      961 gcaatcgata gccggaaagt agctttctta ctcgctactc ctcaacaaga tggcggccaa
     1021 tgggatatga tcgtttcatt attccaaaaa tatggcgttg tgcctaaatc ggtgatgccg
     1081 gaaagtgcga atagttcaaa ttctcgagat ttgaataact acttgaacaa attactgcgt
     1141 aaagatgcag tagtgttacg gaaaatggtt gctcaaggag aaacattaga cgctattgaa
     1201 gaaaagaaag aagaaatgct agaaagcatt tataacttct tagctatctc attaggaaca
     1261 cctccaaaag aattcgattt tgagtaccgc gatgaagaaa agaactatca tcttgaccga
     1321 ggactgacac cacaaatttt ttatgataaa tacatcggtg taaaattaga tgattatgtt
     1381 agcgtgatca atgcaccaac aaaagacaaa ccattcaatc gttcttatac agtagagatg
     1441 ctaggaaatg tcgttggcgg gaaagaagtc aaatatctga atgttgatat ggaaactttc
     1501 aaaaaattag cgattgctca gcttgaagaa ggagaatctg tttggtttgg ttgtgatgta
     1561 ggtcaatctt ctactagaac tagtggcatc atggccttag atgcttattc aatggatgat
     1621 ctatttgata cggattttac aatgactaaa gctgaacgtt tagattttgg agaaagtctg
     1681 atgacccatg cgatggtatt gaccggtgtt gacttagtag acgggcaatc aacaaaatgg
     1741 aaagtcgaaa atagctgggg cgaaaaagtt ggaaagaatg gcttctttgt tatgagtgat
     1801 gcctggatgg acgaatatac gtaccaaatc gttgtaagaa aagatttatt gacgaaagag
     1861 caattaaatg cgttcaatga agaaccaatt gttctatctc cgtgggatcc aatgggtgct
     1921 ttagcataga cgagtatttc aatgaagagt atgtagtaaa gataaaaaga gctggcttaa
     1981 gccagctctt tttatcttta aagtccattc cctgtttttt atggtccctg tgctataatt
     2041 ctaaataaag gataaggatt ggaaataatc cttttatcat taaaaatatg atgacgaagt
     2101 agaataaaat tgggagtagg ggggaaagtc atggatatcc aaactgtttt atctgatctg
     2161 aaattaaatg gtaaagtgat tccagttgtt ggtggcgatg ttaatcagac ctatcgtata
     2221 aaaaccgaac atcgtgctta ttttttaaaa atccatccga atgtaaaaaa aggatttttt
     2281 gaagcagaag tagacggatt aaaagaactg tcagcattcg tacgtgtacc ggatacgtat
     2341 atgctaggtg aaacaagtga aggcgcttat ttgttaatgg aatggatcga gccgggaaaa
     2401 ggggatcaac gagatcttgc tgcagcttta gctaatctcc atcagcaaac agcgccacaa
     2461 tttggttttc gtaaagataa ctatttgggt acacttgttc agaaaaattc ttttgaagaa
     2521 gattggtgga cttttttctt taaagatcgg cttgaatccc agatttcttt ggcagaagaa
     2581 acgaatcgat ggaatgtaca gcgtcaagaa aagtatctgc gatttaaaga acgtgtattg
     2641 aaatcggttg aaccaaaaaa gatcactcca cgcctcttgc acggagacct gtggagtggt
     2701 aatgtgttct ttgaccagca agggcatcca gtttttgtcg acccagctgt ttcttatggg
     2761 aatcgggaac aagatattgc aatgagccag ttattcggtg gtttccgacc ggaattttta
     2821 gatgcttatc aaactatatt tccattagaa aagggatgga aagaccgact gccgatttac
     2881 cagttatatt atcttttggc acatttgaat atgtttggag aaagttacgg ttcccaagta
     2941 gatcaattac tagaaaattt ttaagaggag ggcgtgtttt tggcaaaaaa atataccaaa
     3001 aagcatgaag tttcatatta tgagtgtgac atcaaccaaa cgatgacatt tccttcgatg
     3061 ttaggagtag ttatcaaaac gtctgaagat caaagcgacc agttgggaag aggcagtgaa
     3121 ttcgtcaata gctttggact tacttgggtc atcacgaatt attccatgga gattaatcgt
     3181 ctccctagag tcggtgaagt aatctcagta acgactcaag ccatggagta caataagttt
     3241 ttctgttatc ggaacttctg gatccatgat gaagatggga aagaattagt caaaatagaa
     3301 tctgttttcg tattgatgga ttttgtgaat cgaaaaatga gcagtgttaa tgaagagatc
     3361 attgctcctt ttgagagtga gaagatcaag aaaatcaagc gtcaggaaaa aattgaaaag
     3421 attgattcgg gcttcatgtt gccatatcgt gttcgttttt atgatatcga tagtaatcag
     3481 catgttaata atgcgatgta ttttaattgg atcattgacg tattaggata tgactttttg
     3541 actacgcatg ttccagaaaa agtaattatt cgatttgata aagaagtaga gtacgggaat
     3601 gagatcgaaa gccattttga acaaatccct atcgaaaatg gtgtcaaaac aaaacatgaa
     3661 atccgaatgc aagaacagct gtattgtgaa gcaaatatta tatggaaaaa taaataaaaa
     3721 agaggttgtg acagaagggg gcaacttcaa gaaataagaa gaaatttacg aaaattgttt
     3781 ctcaaatttt tgtaaatttt cgacttagtt ttcgaagaag ctatttttgt cacagcctct
     3841 ctttttaagt ttagctatta gataatgatg tttagttttg gataaacgaa atcaattctt
     3901 cacccgacaa accatagttt acagcaatca atagttttaa acgtgctttt tgactattaa
     3961 tactgttaca gaaaataatc cccatttttt ttagttcttt tcccccgcct tcataatcat
     4021 aaacaggctc tgcgattcca ttgaaacaac gagaaacaag tactacaggg attttttgga
     4081 caagaagctt ttccaaagca gctaaggctt gtggagggag attccccgca cctaaagctt
     4141 cgattaccaa accgtctact ttcgtatggg cgatggcttc caataagtcc ccttgcatcc
     4201 cagcatatgc tttaacgatc gggatagtcc catctacagc ggaaatgtcc agtcgctttg
     4261 tttctaaaag ttcctgcaag aataaaattc gattcttagt tactaagccg atgggaccca
     4321 atgtaggtgt tcggaaagta gcaacatttg tggtatgggt ctttgtcacg tagcgtgcag
     4381 aatggatctc atcgttcatg accaccaaaa cccccttatc tagcgcttct tcacaacttg
     4441 cgacacgaat cgcactttca aaattataaa ggccgtcact tccaagttca ttacttgaac
     4501 gcattgcccc agtcaaaaca attggtaatt tgttgccaat cgttgtatca agaaaaaaag
     4561 cagtttcttc taatgtatct gtaccgtgtg tgatcaccac tccggatata cctgatgcat
     4621 aagcggacaa gatacgttct tttaatacca acatgtgaga aggcatgatg tgtggtgagg
     4681 gaagattgaa gatatcttca actaataatt ctacatgctt ggggattgtg atttctgcat
     4741 ctaataatgg gtttgccaca tcaggactaa caccgcccgt gagcatgttt tctttcatgg
     4801 cgatcgttcc gccggtgtgt agtacaagta tttttttcat tttacagact tccttacgaa
     4861 taattttgca tagccgttaa atcttattgt atactatata aaagagtttt gctatcagtg
     4921 aagaataacg aatagcctaa cttatcataa aaaagctgac cttattaaaa tggaaaagac
     4981 tcaaccttga cctcctaata caatggagta tctggttgag tttcttttac aagaaagcag
     5041 ccaaggaaat caatcacgaa ggtggccgga aatgatcaga atcggattaa catcgtttaa
     5101 agaacatgac aaattaactg gtaaaaagaa taacactctt tatgaatatg cagggtttct
     5161 acccttagtg gaattagata ctgcctatta tggaattccc cgaaagtcct cagttgagaa
     5221 ttggataagt gaagtacctg aatcatttcg ctttgtagtc aaagtctatc aaggaatcag
     5281 cggtcaagga gactggaagc cttattatca gaccgaagaa gagatgattg cgcatttttt
     5341 agatgttatg tcgccaatgg tagatgctgg aaagatgttt tgctttttag tccaattccc
     5401 ctcatcattc aaatgtacga aagaaaatgt ccagtacttg cgcaaactta gaaagtggtt
     5461 tgagggttat cctgtttcta tagaattgcg ggattattct tggtacagcg atgaatttct
     5521 tgaacgtaca agaaacttta tgtctagagc cgctttttct ttagccatca tcgatgagcc
     5581 acagttgctg aatacaacga ttccttttga tccatttgtc accaatccga actttgctct
     5641 ttttcgattc catggtagga atgcgcaagg atggcaagca aatgataaag agtggcgtaa
     5701 aaaaagaacg ttatatcgtt attcagaaaa agaactgcaa gaactagcac atgaagtcaa
     5761 agtagttgat tctaagacaa aagaggtagg tgtgattttt aataataatt ctggtggtga
     5821 tgcggctgaa aatgcattag cactgcaaaa aatattaaat atcaagccag atggactcaa
     5881 tccaacccaa atggatctgt tttaagggct tattttattc aagaaagggg cgaagtctat
     5941 gatcaaagct attttttttg atattgacgg aacattggtc accaatcgtt caaaagcgtt
     6001 ggaatcaaca aaacaagcaa ttgaatatgc tcggaaaaac ggcattttat gcggtgtagc
     6061 aacggggcgc agcccagtaa agatcaaaga aatcattgat gaattggaat tagatatgta
     6121 tgtcgtgtat aatggtcaac tggtttttac tgcagatcgc acgattatcg accacccttt
     6181 tgaacaaaaa gtattggaac atattgttga atttgcagat gagaaccatc gacaaatcgt
     6241 ttttggtgcg agaaatcggt tagatggatc aacgacgatg ctattaggac aatcgatttt
     6301 tatcaagcga ctagtcagct ttttaccgcg taaattcccg gtacgtctga tgaaaaaaat
     6361 cctccaggtt tttagccctc accgacaaaa ggatcgttat gaaacgttag agatattaaa
     6421 agaaccaatt tatcaatgta tattgctcag tccggagtca gaacagaaaa agttagaaca
     6481 tcgctttccc gagtgtacgt ttcagcgttc aaatacgtat actgtagata tcatcccaaa
     6541 aggtggttca aaattactag gcatccaagc tttcgcaaat gctgtcgaaa ttgaaatgga
     6601 agaaattatg gcattcggcg atcattacaa tgatatcgag atgctgaaag gtgtaggaat
     6661 tggagtggcc atggggaatg ctcagatcga agtaaaacat gctgcagact ttgtgactca
     6721 atcaaatgaa gaagatggga tatacgaagg actgaaacac ttcggtctga tttattagtc
     6781 aatgaaagaa ggaaaaaatc agtatggaag cagagacacc ttttcaaaaa gtagaaacat
     6841 tccacaaaac ttttgatcca agaagaccag agatacccac ggcttttaca gaaaatgaag
     6901 ctcatacacg tgctgcgttc aaaatagaag aacttgttga atttgttgca gctacttcac
     6961 ctaataaaga acaactgcat gaagctgtta tgctgctgca cgaagcatta gataaagcag
     7021 aagaaaaaat acttggtaaa acacagtggg gaagtgatcc gctggttgaa caagtagatg
     7081 cactgacaga tatcctttac tttacttatg gttctttttc attactagga atagtgcctg
     7141 atcgaatatt cgaaatcgtc catcaggcaa atatggggaa attatttcca gatggaaaac
     7201 ctcattatga ccctgtaaca aataaagtgt tgaaacctga aaactggcag atggaatatg
     7261 ctccagaacc taaaatcgcg gcagaaatcc aacgtcagat tcaagaagcg attgctaaga
     7321 agaaatccgc tgatataggc atgtgcacaa atgaaaaaga gtgatcagag tttcactatt
     7381 tttgttatct ttatacgatg gaaaaacaaa agattttcgt ttaagttagt gaatgcatga
     7441 aaatagtatg ctataatcta gaatagatgt cttttgcacg agagacgtat tatttggatt
     7501 tatgccaagc agaacgccat ctgttgcata aaagatgcat actgaaaacg gaggaaccat
     7561 aaatgggaaa taatgttatc atcggaatcg tcattgcaat cattatagtc gccattgtcg
     7621 tctttttggt tagttacttg atgaagaaaa aaaaccaaga tcgattggat gccttagaaa
     7681 aacgcaagga agaattattt gacttgccag tgattgaaga agtcgatgat gttaaaaaaa
     7741 tgcatctggt tgggcaaagc caaaatacct ttcgtgaatg gagtcaaaaa tggaatgaca
     7801 tatctacttc ctcgttcgct gaactagaaa gtcaaatttt cgaagtggaa aacctcaatg
     7861 aaagttttcg gttcttaaaa ggaaaaaaag cagttgttga agcagaagct acaatggacc
     7921 aaatggagaa acaagtgacg gaaatccgtg aaggtctaaa ggaattgcgg gaaacggaag
     7981 aacgcaactc actagctgtt cagcaggcac ttgacgtcta tgaagaactg aaagaaaaag
     8041 tcagagaaga cgagcatgaa tacggaccag ctctttttga attgcaaaaa cagatcaaag
     8101 tgatcgaaaa agaatttacg cagtttgtga ctttaaacac ttctggagac cctgttgaag
     8161 cccgcgaggt tttggataaa gcagaaaaac atacgtatga agtggaagct ctgatgaaaa
     8221 aaattccacc gctgtatgag gagctccata ccactttccc tgaacaactt gaagaaatca
     8281 gtgatactta cgagaaattg atggaagaac aatatgtatt cccagaagaa gatcttgctg
     8341 aagatatcgc aaaagtaagt cgaagaattg aaaattcatt agccaatctt gaaaaaacag
     8401 aagttgaaac ggtcgaattt gaaaaccgtg aaacagctga tttgattgat tcgttatatg
     8461 atatcttaga acgggaaatg gaagctcaga gatatgtaaa aactaaccaa tcaacgattg
     8521 cagagtatat caaacataca acaaagaata accgtcaatt attgattgag ctggatcaca
     8581 catctcaaag ctatacattg aaccacaatg aaattggccg tgtacgaggt ttccaaaccg
     8641 aagtggaaga aatggaacgt caaaatgaac agatgattcc acagatccgt caacacgaaa
     8701 ttccttattc agaagtgcgc acgttctata aaacagtctt caaagtctta gaagatatcg
     8761 aaaagcaaca agtagagatc gacgactcat tacatgaatt gagaaaaggc gaaaaagaag
     8821 cgcaagaaaa gatcgataca ttcgaattca aattgcgcag tttgaaacgt tttgtagaaa
     8881 aacaacgttt gcctggcttg ccgaatgatt acctagaatt cttttttgtt gctactgacc
     8941 gtattgaaga actaagtgtt gtcttgaata aaattcgagt aaacatggaa gaggtcaatc
     9001 gtttagttgc tctatgtgaa gaagatctag aattattaga taagaagaca catgatttag
     9061 tagatgcagc agcattaacg gagcaaatgc tccaatatgc aaatcgttat cgtcatacac
     9121 acgaagaagt gagagaagca attgataaaa gctactattt attcaataaa gaatatcact
     9181 atcaagaagc attagacgaa atcggtacag cattagagcg agtagaacct ggtgctttca
     9241 aacgcatcga agatttttat tttaatcatc cagacttagt ataaagcaaa aaagttagta
     9301 aaccagcaag agcaagacgg attattgtct tgttcttgtc tttttttgta tatttgaaaa
     9361 ttgaaacaag tgaaaatttc acctgctctt gaaagcacct tggacaaccg ttataataga
     9421 aaagaatttc aaaaaagagg ggtttcttat gatatatttt gataacagtg caacaacccc
     9481 aatctttccg caaagcttag atgcatatgt aaaaaccagt cagcggatta ttgggaatcc
     9541 atcaagcttg catgatttag gcaatcaagc taaccggttg ttgcagcaag caagaaagca
     9601 gatcgctgat ttgatccatg tagaaccaga agaaatctat tttacttcag gcggaacaga
     9661 aggagataac tgggtattaa aaggaacgat gatcgaaaaa agagaatatg ggaatcatat
     9721 gatcatttca ggagtcgaac atccggccgt cagcgagaca gcagaacaat taaaagaatt
     9781 aggaatcgaa gtaagtatcg ccccagtgga taaacgcgga tttgtacgtg tcgacgaatt
     9841 gaaagaatta ataagaaaag agacagtatt agtctctgtt atggctgtga ataatgaagt
     9901 aggatcgatt cagcccatcc aggaaatcag tgatcttttg gatgattttc ctaaaattca
     9961 tttccatgta gatgctgttc aggcgattgg aaaagtacca ttctcgcaat ggttgacaga
    10021 aagagttgat tttgcaacat tttcagctca taaattccat ggcccacggg gaactggttt
    10081 catttattgg aaaaagggtc gtcgtttagc accattactg acaggtggag ggcaagaaag
    10141 aaatcagcga agtgggacag aaaatgttcc aggtattgtg gctatggtta aagctttacg
    10201 tttatcatta gagataaagg aacaaaaacc tgagcagaca caacgcttaa aagtagctct
    10261 aatcaatgcg ttgaagacgt atgagaaagt gacgattttt tcagaaggaa ctgactatgc
    10321 accgcatatt ttatgttttg ctctaaaagg aattcgcggc gaagttctcg tccatgcctt
    10381 agaagaaaaa caaatatttg tttctactac tagtgcctgc tcctcacgta aacatatggc
    10441 aagcagtaca ttgcacgcaa tgcatgtacc aaatgatctg gctacatctg ctgttcgaat
    10501 cagcttagat gaaaacaatt cgcttgcaga agtcgagcaa tttatgatta ttttcaagca
    10561 gctttataag aaattttctg caatcaatta aaagaggagt gttcttgtga aatacagtga
    10621 aattatggtc cgttatgggg aactttcgac aaaaggaaag aatcgaaaaa gctttatcat
    10681 gcaactagca caaaatgtca gacaagcttt gaaagacttt ccagaaatca aaatccatgc
    10741 tgatcgggac cggatgcatc ttttattgaa tggcgcagac gggcagctag ttattccaaa
    10801 actagcaaaa atatttggga tccaaaattt ctctccaagt attcgtgtag aaaaagatgt
    10861 cgaagttttg aaacaagctg ttcaagacat catgaaagaa atttatactg ggaaagaaac
    10921 atttaaaatt atcgctaaaa gaagcgatca ccaatttgaa ttgacaagta atgaattgaa
    10981 tcaaacatta ggtaatgctg tttttgatat tttcccgcat atccaagttc aaatgcgtca
    11041 accagatatt ccattacgtg tagagattcg gcgtgatggc gcttatctat cgtatgaaac
    11101 gatcaaggga gctggaggat tgcccgtagg aacaagcgga aaaggaatgc ttatgctttc
    11161 tggggggatt gattctcctg tagcagggta ctttgccatg aagcggggag tggaaattga
    11221 agctgttcat tttgctagtc cgccgtatac aagtgaacaa gcactgcaaa aagcaaaaga
    11281 tctaacagcc aaactagcac cttatgtggg aacgatccat tttatcgaag tgccatttac
    11341 cgagattcaa gaagaaatca aaaagtcagt gccacaagga tattggatga caattactcg
    11401 tcgaatgatg ttgcgtttga ccgatgaaat tcgccgtatt cgtcatggtt tagtcatttt
    11461 gaatggtgaa tcattaggtc aagtggcttc acaaactttg caaagtatgg tggctatcaa
    11521 tgaagtaacg aatacgccaa tcattcggcc tgtagcgaca atggataaac ttgaaatcat
    11581 tgaagtagca gaacagatcg atacatttaa tctttctatc cagccttttg aagattgctg
    11641 tacgattttt gctcctccac agccaaaaac acgccctcaa ctggaaaagg tccatcaata
    11701 tgaagagcgt ttagcaatag atgaaatgat ccaacgagca ctagctggct tgaagatgga
    11761 agagatacaa gcagaatcgt ctaatgaaaa aactgaagaa ttttcagaat tgctataaga
    11821 ggttatgaaa atagtgtttt gaccagagta ttagagaaac aaaacgaaaa aatagctcca
    11881 tgtattttac cgtttttgaa ctaatgtcga agattagctg attgaatacc atttatcaag
    11941 aagttatgga aaagccggtt cttttttact aactgaaaaa taaaatttta tttagagatc
    12001 aagctacagt tgataaatga tataaaaaag agaatttccc atgaaatagc gaggttgtga
    12061 cagaagtggg cagcttcaag aaataagaag gaatttacga aaattgcttt tcaaattttt
    12121 gtgaaatttc ggcttatttt tcgaagaagt tacttctgga acaccgtgta tcagaggttg
//
LOCUS       DAKBVG010000074        12096 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000074 DAKBVG010000000
VERSION     DAKBVG010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12096
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.074"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            1..624
                     /locus_tag="OUD09_001413"
     CDS             1..624
                     /locus_tag="OUD09_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9692688.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1074..2123
                     /locus_tag="OUD09_001414"
     CDS             1074..2123
                     /locus_tag="OUD09_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCT9692689.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2120..3001
                     /locus_tag="OUD09_001415"
     CDS             2120..3001
                     /locus_tag="OUD09_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT9692690.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3004..3774
                     /locus_tag="OUD09_001416"
     CDS             3004..3774
                     /locus_tag="OUD09_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCT9692691.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4053..4907
                     /gene="aroE"
                     /locus_tag="OUD09_001417"
     CDS             4053..4907
                     /gene="aroE"
                     /locus_tag="OUD09_001417"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCT9692692.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5085..6248
                     /locus_tag="OUD09_001418"
     CDS             5085..6248
                     /locus_tag="OUD09_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCT9692693.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6365..6775
                     /locus_tag="OUD09_001419"
     CDS             6365..6775
                     /locus_tag="OUD09_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCT9692694.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6949..9177
                     /gene="topB"
                     /locus_tag="OUD09_001420"
     CDS             6949..9177
                     /gene="topB"
                     /locus_tag="OUD09_001420"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCT9692695.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9348..9632
                     /locus_tag="OUD09_001421"
     CDS             9348..9632
                     /locus_tag="OUD09_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9692696.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9792..10028
                     /locus_tag="OUD09_001422"
     CDS             9792..10028
                     /locus_tag="OUD09_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCT9692697.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10025..10423
                     /locus_tag="OUD09_001423"
     CDS             10025..10423
                     /locus_tag="OUD09_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HCT9692698.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(11090..12034)
                     /locus_tag="OUD09_001424"
     CDS             complement(11090..12034)
                     /locus_tag="OUD09_001424"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HCT9692699.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
BASE COUNT         4144 a         2057 c         2460 g         3435 t
ORIGIN      
        1 gtgtttgtta tttgggaagt agttaaatcg agagtacaag aaatgaatga acatttctca
       61 gtgtcattcg cagaaaatcc aagtaagtat tttcgacgtc ttgtttttct gctcatctta
      121 tcattgctgt taacagttgc aatgttttta ttgccggaaa aaatccgaga agagcaagta
      181 aatagtctcg tttattcgtc cataccaacc agcaaattga aaccttttga ttacagcaaa
      241 gctgatcaga aaatcaaaca aagccgggcg atatcggtga tgttcagcaa accgaacggg
      301 aaaacctatc aggacatgct tgatgttttt aatgatcctg agcttatgga agaattgaat
      361 cgaccgatct tttattatcc gatcgtttat gatgtacatg aactggaaca aaaatataac
      421 atacgaacgg atgaagtgac atttattttc tttgatggag gaaaagaagc aaatcggata
      481 actgcgggta agggaggaat aactgatttt gataaggagt tgatccctga actgaatcga
      541 cttccgttag ccaatatcaa acaattagaa gaagaattgg atagaacggg agtaacttcc
      601 caaaattccg agattgctaa ttgagacatt ggcgcgatga aacggtgggt ctatatagcg
      661 ctatttttaa ttatcagaac aagtgatgtt tgatcttcaa aataacaaat aaaaaattaa
      721 aaaatatagt tgacgaaatt caaaaattcg attagactta aatcaattct taaggaaagg
      781 gtgacctgta atgaacttat caaaacgtat ttcagcagaa aacaaaaaaa attggcgcag
      841 ctggcgtaga taagttgtat taatggtgag actttcccat agatacaact ttttagagtg
      901 catttctaaa gcttgtatct atgctagaca tgaacattga ctgccgcata gattagaatc
      961 tatgcggttt tttgtgtaaa gacaatgacc gctttggcca ttgtcttttt ttgtagactg
     1021 gatacttgcc tgattataat aaataaaaag attgactttg caggaggaaa aaaatgaaaa
     1081 acaaacgatt aataacagtt gtagtaatta ttttagttta tttagcaggt gcatttattt
     1141 atggaaaagc ctcaggcgaa aaagaaagta cttcgacgaa ccagacaaaa aaaacaacag
     1201 ttgttggggt attgcaatac gtgagccatc cggcattaga tgagatttat cgaggaatca
     1261 aagatggtct gaaagaagaa ggattaaaag aaggagaaaa tcttgagatc aaatttcaaa
     1321 atggacaagc cgatcagagc aagctagcca caatgagtca acagctagta caatctgatc
     1381 cagatgcctt gattggaatc gcaacacctg ccgctcaatc attagcaaac acgacttcat
     1441 cgatcccagt tgtgttaggt gctgtaacgg atcctgtggg ggctggatta gtcgcttctt
     1501 tagagaaacc tggtggtaat gtaacaggtg tttctgatca gccgcctgtt acttcacaaa
     1561 tcaagcttgc ttctgatttg ttgccaaatg caaaaaaagt cggaatcttg tatgcttctt
     1621 cagaggataa ttcaaaatac caagtagcac aagcagaaga agctgcaaaa aaagaagggt
     1681 tagaatcagt aaaatatgcg gtgccttcca ctaatgagat tgcacaaacg gttcaagtga
     1741 tgagccgaca agtcgatttt atctatgttc cattagataa taccattgcc aatgcgatgc
     1801 aaacagtggt aaaagaagca aacaaagcaa atatccccgt cattccatct gtggatacaa
     1861 tggtggaaca aggtggacta gcaacaatcg ggatcaatca atatcaatta gggcttcaaa
     1921 gcggtaagat ggcggcagaa cttgcgagtg gaaaagaaaa accggaaacg acgcctgttt
     1981 atacatttga tcaaggggat acagtgatca atcaatctca agctgatcgc ttaggaatca
     2041 ttgttcccca atcgatgaag gaaaaagcca aaatcattac agatgactcg cagcaagaaa
     2101 catctaagga ggatgacaaa tgatcgtatc agctatttca caaggcatgc tttgggcaat
     2161 attaggatta gggattttca tgacttatcg gatcttagac tttcctgata tgacgacaga
     2221 agggtccttc ccattaggtg ctgctgtctg tgtgacagct attactcatg gggtagcgcc
     2281 gattgtagct acattattag gcgtaggtgc tggcatgata gcaggactcg ttacggggtt
     2341 actttataca aaaggaaaga taccggttat tttagcaggg attttagtga tgtcaggatt
     2401 gaactcagtt attttgtttg tgatgcgaac accaaatctt tctttattga atcatccgat
     2461 tctacaagac agcttccatc gctttgggtt acctgattac tttgacacag tccttcttgg
     2521 cttcatctct ttgggcatct tgattgctgt actgctattc ttcttcaata cagatttggg
     2581 tcaaggctat attgcgacag gagacaacga aacgatggct cggtcgttag ggataaaaac
     2641 cgatcgtatg aaaattttag gtttaacttt gtcgaacggt gtcattgcat tatccggtgc
     2701 gctgattgcc cagaacgacg gatatgcaga tgtaaataaa gggattggcg taatcgtcat
     2761 cggtcttgct tccatcatca tcggtgaagt gatcttccaa gaattgacgc tagcagaaag
     2821 attgattgct attgttgtcg gcagtatcat ttatcagcta ttgattttag tagtaatcaa
     2881 actagggttt gacacgactt atctgaagtt gttctccgct atcatcttag ctatttgtct
     2941 gatgatcccg caattgaaga aagcactgaa cttaaaaacg ggttttgaga aggaggcata
     3001 gtaatgactg atatattgac aatcaaaaat ggcagaaaag tcgtaaacaa tggaatgaat
     3061 gaagagaaag tactattgga tgaaattaac cttacaatca gagaagggga cttcatcact
     3121 gtccttggcg ggaatggtgc tggaaaaagc acactgttca atacgatcgc tggtacgctt
     3181 caactcagtc aaggatcgat ctcttttaaa gatcagctga tcacaaaaga ttcagaagaa
     3241 aaaagagcac gattcctttc tcgagttttc caagacccca agatgggaac ggctcctcgg
     3301 atgacagtag cagaaaattt gctacttgca caaaaaagag gtcaaaaacg gaaattccgt
     3361 ttacgtaact tgaaaaatca aaaagaagat tttttcagac tatgtcaaga agtcggtaat
     3421 ggcctagagc aacacttgga tactccagcg ggtgaattat ctggtggtca acgccaagca
     3481 ttaagcttgc tgatggcgac gatcacagct cctgagttac tattattaga tgaacatact
     3541 gcagctttag atcctaaaac agctaaagca ttaatgcgat taacaaatca gcggatcaat
     3601 gaacaaaaat tgacttgtct catgatcacg catcgaatgg aagacgcctt gaaatacgga
     3661 aatcgtctga tcgtactcca aaaagggaaa atcgttcaag atatttctga aaaagaaaag
     3721 caacatttga caatgggcga ccttttgcag ttctttgaag aagtagtgga ttgatttcga
     3781 cagaaaaatt cctttcagta gaaggacttg aaaactcatt gacatcttcc tttgattcat
     3841 cctatactta aaacaataaa caattaaaca tatttgggtg ttttgtagga tcacgtagtc
     3901 aatgatttta acaattaaat agaatattct ttgctctgac aagtaaacga tgagaaaaga
     3961 atttgctact ttgccaccta gacgtttcgt actttttcgt atcaaacgct aagtggcttt
     4021 ttttattgaa gtaagaacaa aggaggaata agatgatttc tggacaaacc aaattagctg
     4081 ggctctttgc tgatccggct tctcacagtt tatccccatt gatgcacaat actgcttttg
     4141 aagcaaacaa catcgatgcg gtgtatttag ctttccaagt aaatccaatg aatttgaagc
     4201 aagcagtcga aagtattcga acttttgata tgttaggtgt aaatctttcg atgccgaata
     4261 aaacagcggt tattccttat ctggatgagg taagccagga agcgcaattg atcggagcag
     4321 tgaatacgat cgtgcacgaa aagggtcgtt tgattggcta taataccgat ggtgcgggct
     4381 tcatgcaatc cgtgcaagaa gctgggattt cgatcaaaaa gaaaaaaatg actctactgg
     4441 gagctggagg tgcagcaaag gcagtcactg ttcaagctgc gttagacggc gctgaggaaa
     4501 tcgtagttta taaacgaaac aaccaaacat tcgctcaagt aaaagaaaca ttcgaacaac
     4561 tcagctatcg aacttcttgt aagataaaag ttcgtgactt tgccgatacg catcaattaa
     4621 gaaaagactt gaaagaaagc tgtcttctag tcaatgcaac agatatcggg atgggaaaga
     4681 aaatcaatca gtcaccgctc ttagatataa gcatgatgca tgagaatcta gcggtttttg
     4741 atttgattta tgcacctaga gaaacacgtt tattaaaaga agcaaaaaaa gtaggagcaa
     4801 aaggatacaa tggacttggc atgttgatct accaaggagc aatcgcgttt gaattatgga
     4861 caaaggaaaa gatgccgatc catgtgattg aaaagctgtt tgattaaaaa aggataatag
     4921 ataaagatct tttattctcg cataaacggt ctcaagaaaa gtaatccttt atgtatcgaa
     4981 gcatttattt cgttattttt tattaaatta tcaaaaaatg ataggctttt gtcgattggt
     5041 tatcgtatcc tttatttatc agtaagaaac gaggaggaac cacaatgagt cggatggatc
     5101 gctataagga catccataag aaggcgaaac ctctgaagcg ggatacgggg agtatttttg
     5161 ctagaagaga acgaaaacga acgcgtatgg aaaatgagga agatgctacc cgtatttatg
     5221 aaagagagca gcattcattt gaacatttga accaatacga gcaacagata tacagagatg
     5281 caccaagagc agaagaacaa caaaaaaata aaaaattttt caatcggcaa aagaagaaca
     5341 aaccgttaaa acctcgaaga aagcgatcgt ggaaaaaaat cattctatcg attttgcttg
     5401 ttttgatcct ttattcaggt atctcatttt ttattggtaa atatgtagct gaacatgaca
     5461 caagcttaga ggcagcgact tcagaaacct ttcatgggca aaaagcgacg aatggtgcgc
     5521 ataatatttt gatccttgga agtgatacac gcggagaaga tgccgggcga gccgatacga
     5581 tcatggtctt acagttggat ggtccttccc ataagccgaa actgatttct tttatgcgtg
     5641 acagcttcgt tacgatccca ggggtaggac aaaataagat caattcagct tatgcatacg
     5701 gaggagccga tctagtgcgt caaactttag tagaaaattt tggaatcgat tgtcaatact
     5761 atgcgaaagt cgatttcaaa tcgtttgaaa aagtgatcga tgccttattc atgaacggag
     5821 tgaagatcga tgcagaaaaa gatctgaatc tggatggtgt agatattaaa aaaggggtcc
     5881 agaagatgga tggacatgtt cttttgcagt acgcacgttt ccgaatggat gaacaaggag
     5941 actttggccg tgtcagacgc caacagcaag taatgaacgc tattttcagc caattgaaga
     6001 atccattgaa tctgatccgt gcaccttatg cagctggtaa agctttagga tatacatcca
     6061 cagatgtcac tgcttggttc ttgacgaaaa acctcttttc gattgcacga ggagctggtg
     6121 gcattgatcg aataagtgtg ccagtagacg gttcgtggaa ttatgggcaa agtgattatg
     6181 ctggcagtat tcttgaaatt gataatcaaa tgaatcaatc tgctattaca gattttttga
     6241 ataaatgatc ataaaaaaga tcaagaagaa gctgcgcaag gcttttgtca tgaatcaatc
     6301 aaacgattca ctcgtaaaaa acgctttctt atggtagatt taaccaagag aggagctgtt
     6361 tacattggat atctatctga acttcaaaaa tgagtcgcgt gaagccatcg ctttttatga
     6421 aaaagttttt cagactgaag catcaggtat caccacatac ggtgaaatgc ctggaataga
     6481 acaaatgaac ttgccagaag aagcaaaaaa tctaatcacg aatgcacggc tgaacattta
     6541 tgatacatta gttatgtttg cagatacgcc agaaggaatt ggtcctgaat tagttgtggg
     6601 aaataatgtt tctttagtca tccagacaag aaatgaagaa gaaacgaagc gcctatttga
     6661 aagcttagct gagggtggta agattgagat gccactggat gctgttccgt gggctcagtt
     6721 gtatgggatg gtaactgatc gatttggcat caactggcag attaaccaag agtgacagct
     6781 aaagatgaat attttctgga aacatcaagc agcaaacgga tcatcgtttg tttgcttgat
     6841 gtttttgtgt ctatttcgac tatacggtat ctttttttta ttgccagatg cgaaaatatg
     6901 ttccataata gagaagtccg caaaaaatta agtaacggag gtcagaagat gaagacggtg
     6961 atactagctg aaaaaccttc acaagcaaaa gcctatgccg aaaccttcca acagcaaaag
     7021 cggcatgaag ggtattatga aatcagcgat ccattgtttg ctggtgaagt aaccatcact
     7081 tatggttttg gacatttggt agatatggtt gctccaggag cttatgaaga gcgttgggca
     7141 aaatggtctt tagaaaattt gcctattttc ccagaaacct tccgttacga agttccaatg
     7201 gacaaacgtg cacaattttc gatcgtaaaa cgagaattgc agtcagccga taccatcatt
     7261 attgcaacag atggtgatcg agaaggtgaa gccatcgcgt ggtcgattat tcgacaagca
     7321 aacgcatttt ctaaggaaaa gaactattta cgtttatgga tcaattctct tgaaaaagaa
     7381 gcaatctatg aaggttttaa gaatctccaa cctggaaaga actattttcc aaaatataaa
     7441 gaagcgcaag caaggcagaa cgctgattgg ctgattggaa tgaatggaag tccattatat
     7501 agcttgctac ttcaacgaaa agggattgat ggcagttttt ctcttggtcg tgtacaaaca
     7561 ccgacactct atatgattta tcagctgcaa gaagaaatca aacacttcaa aaaagaaacg
     7621 tactttgaag gagaaggttt aattacgaca aacaaagggc agttttctgg aaaaatcgta
     7681 ccaaaaaaag catttaaaac ccaagaagag ttaacgaagg aaatagaaaa acttggagcg
     7741 catttaggta agcaaaacgg acggattctt gaagttacga aaaaggaaaa gaggttgcat
     7801 agtccacgtt tattttcgct atctagtttg cagacaaaga tgaatcagct aatgaaagca
     7861 agtgccaaag ctacacttga tgctgctcaa ggattgtacg aaagcaagtt tctaagctat
     7921 ccaagaacgg attcctttta cattacagaa aatgagcatc agtatctggt taaacatctg
     7981 caaaattata aaaaattttt aggaatagaa gaagtacaga caactgagat caaagcaaag
     8041 aaacgatatg tcgatgcgaa aaaagttcaa gaacatcatg caattattcc gacaaagacg
     8101 attccgactc ctgcacgctt tgcgaagctt tccaaattac agcaagcaat ctatttgcaa
     8161 gttttgcgca ctactgtggc tatgtttgct gcagattata tctacgaaga gacgaccgta
     8221 atgacgggcg tacagcaatt aaagctaaaa agcatcggaa agatcccatt acagcaaggt
     8281 tggcaggaaa tcttacgcat caagtcaaaa aagaaagaag ctcagactct tcctcctatc
     8341 gtagaaaatg aagaagcaga agtagaactg aaaagtatgg agaaagaaac acaacctcca
     8401 aaaccctata ccgaaggaac gttgatcaca gcaatgaaaa ctgcgggaaa aacgctggat
     8461 aatgatgaag cgcaagaaat tctcaaagaa gtagaaggaa tagggacaga agctacacga
     8521 gccaatatca ttgaaaatct taagcagaag cattacatcg aagtcaataa aaacgaaata
     8581 actgttacgc caaaaggaat cactctttgt aaggcagtag ccaatgaacc attactgacg
     8641 agcgcagaaa tgactgcgcg ctgggaaggg tacttgagaa aaattggtaa acaagagggg
     8701 acaccatctg tttttttaga gaatatcaaa aaattcatcc tacatttatt ggcagaagtt
     8761 cctaatcaaa tcgacaaggt agacttttca gatgagatta gcgggacacg tcagataaaa
     8821 gcgatggaga agaaaaacga tcaattagga agatgtccaa aatgtaaaaa gggaatagtc
     8881 atgctttatc ctaaaatcgc tacatgcttg aatccggaat gcgacttcaa attgtggcca
     8941 acagtagcta aaaagaagct gactaaaacg aatctcagag atttgctttc aaaaggaaag
     9001 acaagtaaag ttgtcaaagg ttttactggt aaaaaaggga aattcgatgc agttttagta
     9061 ttaaaagaag atatgacaat cggtttttct tttccatgga tcgaaaacag tggaacaaaa
     9121 gcagttgatg gaaacaaaga aacatcaaga aaaaataatg aaaacgaagt agagtaaatg
     9181 aaaacaatga aatagcggaa tatttattcc gaaaagtgag aatataaata ccaataaaac
     9241 taaaaaatat cagtgaatca agcaaaaacg ttggggtagg ctgattttat atagatagca
     9301 aggagttttt ttatttgaaa aaagtgcatt catttttaaa ttatcttatg ggagcaatca
     9361 cgattgtggc tgtgtaccaa gcatttctaa agtatcctga ttacgggacg aagttgttgt
     9421 cattgacgca caatcaaaca gcatattcac caacgttgat tttatcgact ttgactgcag
     9481 gagctaaaag cgctacttat ttttttcata aaacgacaag catgctaaag cgaaagaaac
     9541 agcgaaaaca attgttggtg gtggtagcgg ctatgatcat tatttatttt ctaccagtta
     9601 ttacacaagt atttcatcaa tattttggtt aagaatacga gagtttgtga caaaagtcat
     9661 gtcacaaact tttttctttg tcattttttc gtaagaaaaa gcaaaataaa agtatataca
     9721 ttgattaaaa gtatatactt ggatataatg tacttactat agaatgagaa tggtatagag
     9781 ggtggcgaaa tatgaaaaaa atgaaaaagt ataaaattag aaaatctggc aatagcgatg
     9841 taacaactat cccaccagaa gtaaaagaat tcatgggtgt gcagacaggt gacgcaatca
     9901 gttatgtttt tcaatctgat ggttctgtac gaatgatcaa agcacaagaa gaacctgata
     9961 ttgattcatt agttgattcg atcatgaatc aatatgaaga cgcgttaaaa gatttggttg
    10021 atttatgagg tatctttcga ctaaagaaat tatcaaaatc aacgcgaaag ttattttacg
    10081 tcactcgcca ggcgaaatga tcggtgtaaa agatgcaaat gcattagata tggccgtcaa
    10141 acagccttca caagcagtat tcaatcaaga actctatcct gaaatctatg aaaaagcggc
    10201 gattttagca attaatttag ctaaaaaaca tccttttcat aatggcaata aacgaactgc
    10261 tcttgttgcc atgttgttgt ttcttcaatt gaataattgt ccagttgctt tttctcgtca
    10321 agaagctgtt gattttatca taatgattac cactagttca ttggatttcg attcactaaa
    10381 gtccaagata accagttatt tacgacaaac cgcaataaaa tgaacgaatt aaaaacttta
    10441 tttctcaatt agatcagcat gtcattaagg aatatgctga tttttttatg tttaatcata
    10501 caaaatagtt ttttcataaa aatatggaaa atatggatta attttgaaat aattgattta
    10561 aaaggaatac aaaagagtga atagtaggaa atagtggttt agaactagtg ggctgatttt
    10621 ttatatattt tagtttacat aatatatatt atacaaagta gatttaaaaa atgataaacc
    10681 tctctcgaac ggttttttat caaaaatcgt catttttcca tcaaaatctg cttatgcaaa
    10741 attataaaaa aattttgcag tttttcttac tttttttgtc gtttaattga atgataaaaa
    10801 ccagtttctt tattcagtta atcaaatatg aacgaattag atatgaatca ttgtatttgc
    10861 cgttgcggaa ggatttttta attaaccaca gataaacatt gataacgatt cgcttattgt
    10921 aatcaacaga ttaaatttca tatgttcata tgtacggata cattttcgga ccgattaatt
    10981 ttaaccattc tgtttatttg cttacctatt agatatcaat aaaaaactgc ttaacagcta
    11041 tttcagctgt taagcagttt taggattttt agttcatatt cattgtttct tataatacag
    11101 tgttcaatac ttctcttaaa gcacttgttg atttgctgaa cattgctttt tcttcttcgt
    11161 tgattgataa ttcaacaact tcacgaacac cattttcgtc aacgattgat ggcacacctg
    11221 taaagatatc tttttcgtcg tattcaccag ttaaataagc agatacaggc aatacagctt
    11281 gttcgttatt caagatagct tttacgatac gtgcagtact cataccaata ccatagtaag
    11341 ttgctttttt acgatcaatg atctcataag cagcattacg gactttatca gcaatcacat
    11401 ctaattcatc ttgggcaata cgatggtctt tttctacaat ttcaaacact ggtttaccac
    11461 cgacagttgt atgtgaccat gcagcaactt ctgaatcacc atgttcacct aagatatagc
    11521 cgtggacgct gcgtgggtca acttttaatt tcaatgcgat ttctttacgg aaacgagttg
    11581 tatccaatgt tgttccagta ccgataacac gtgatgttgg cagaccagat tcgttccaag
    11641 cgatatatgt caaaatatct actgggtttg aagcaacaac gataataccg tcgaatcctg
    11701 atcccatgat ttcttttacg atttggcgca tgattgacgc atttgtttta actaaatcta
    11761 aacgagattg accaggtttt tggttgatac cagctgtgat aacaacgata tcagcatctt
    11821 tacattcttc atagccacct gaccatacag ccacattttc atcgccccaa gccataccat
    11881 ctaaaaggtc caatgcttcc ccttctgctt tttcttgatt cacatcgatc aatactaatt
    11941 cattagagat tccttggttg atcattgcat aagcgatact tgttccaaca aaacctgtac
    12001 cgacgattac tactttacga cttgtttttt tcatgaataa acagctcctt ttcataacta
    12061 tttgttactt tcatgaaaag tatagtcttg tttatt
//
LOCUS       DAKBVG010000075        11907 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000075 DAKBVG010000000
VERSION     DAKBVG010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.075"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            364..633
                     /gene="rpsN"
                     /locus_tag="OUD09_001425"
     CDS             364..633
                     /gene="rpsN"
                     /locus_tag="OUD09_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCT9692700.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            648..827
                     /gene="rpmF"
                     /locus_tag="OUD09_001426"
     CDS             648..827
                     /gene="rpmF"
                     /locus_tag="OUD09_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCT9692701.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            859..1236
                     /locus_tag="OUD09_001427"
     CDS             859..1236
                     /locus_tag="OUD09_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9692702.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1257..1406
                     /gene="rpmG"
                     /locus_tag="OUD09_001428"
     CDS             1257..1406
                     /gene="rpmG"
                     /locus_tag="OUD09_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCT9692703.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1430..2413
                     /locus_tag="OUD09_001429"
     CDS             1430..2413
                     /locus_tag="OUD09_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCT9692704.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVIADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLEKFNHPLVFESDSSENH
                     YNGKHYHQHHQEHEFKSILLTSDQDIKKDFLFQLIDRLILTNQGSLYRFKGLIALQDQ
                     DFIVAIQGVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2443..2565
                     /locus_tag="OUD09_001430"
     CDS             2443..2565
                     /locus_tag="OUD09_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HCT9692705.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKQNKMKKLA"
     gene            2583..4106
                     /locus_tag="OUD09_001431"
     CDS             2583..4106
                     /locus_tag="OUD09_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HCT9692706.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLIQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKLNKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNEMHNWPTFYPASLSEHEIAQEMMAH"
     gene            4130..4369
                     /locus_tag="OUD09_001432"
     CDS             4130..4369
                     /locus_tag="OUD09_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCT9692707.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYQFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4562..5197)
                     /locus_tag="OUD09_001433"
     CDS             complement(4562..5197)
                     /locus_tag="OUD09_001433"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCT9692708.1"
                     /translation="MVRHLFVDFQWSPEKISNRLKHEHMDFSISFITIYRAICNDLFD
                     TEPLSHGNKGLIRSLHHRGKTRHTKNHVESRGKICLTHSIHGHPKSADDRSDFGHWKA
                     DIVAGKTSGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEVLNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5218..5436)
                     /locus_tag="OUD09_001434"
     CDS             complement(5218..5436)
                     /locus_tag="OUD09_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCT9692709.1"
                     /translation="MSQYCHLTILKRERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTNHLPKRKKIMLIEEKNADVITF"
     gene            5562..6766
                     /locus_tag="OUD09_001435"
                     /pseudo
     CDS             5562..6766
                     /locus_tag="OUD09_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180763.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
     gene            6761..7168
                     /locus_tag="OUD09_001436"
     CDS             6761..7168
                     /locus_tag="OUD09_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HCT9692710.1"
                     /translation="MKFLRKAGVKIGDVIIIGILIIPSFIPLFVFSIEKENETTEDAI
                     KYAIVQIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVR
                     TGWISKPGQTSICLPHKLVISIEKKESKDYYIY"
     gene            complement(7409..8749)
                     /locus_tag="OUD09_001437"
     CDS             complement(7409..8749)
                     /locus_tag="OUD09_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HCT9692711.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
     gene            complement(9025..9180)
                     /locus_tag="OUD09_001438"
     CDS             complement(9025..9180)
                     /locus_tag="OUD09_001438"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HCT9692712.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLTDHHITHSMSQTGTPVDNAVIE
                     AFHRSIK"
     gene            complement(9364..9567)
                     /locus_tag="OUD09_001439"
     CDS             complement(9364..9567)
                     /locus_tag="OUD09_001439"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCT9692713.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKMLNR"
     gene            complement(9477..9809)
                     /locus_tag="OUD09_001440"
     CDS             complement(9477..9809)
                     /locus_tag="OUD09_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCT9692714.1"
                     /translation="MKKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            complement(10608..10799)
                     /locus_tag="OUD09_001441"
     CDS             complement(10608..10799)
                     /locus_tag="OUD09_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCT9692715.1"
                     /translation="MNTLKEDFILAKNGNEEAVVAILKRFSALVHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            complement(10897..11283)
                     /locus_tag="OUD09_001442"
     CDS             complement(10897..11283)
                     /locus_tag="OUD09_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCT9692716.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFNKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
BASE COUNT         3955 a         1868 c         2282 g         3802 t
ORIGIN      
        1 atcaaagttt taaaaaattg gcgataaacc agttaaacat aaaagattga ataaaaagtt
       61 atagtttctt tcatattgcc gttttaagta gaggaaaagc tacttaaaac ggtatttgtg
      121 tttgattgga ctaataaaag tgaattctta tttttcagag cttaaactgc atattctttt
      181 aaatgatttg attatctcta tgtaccgata aattcgtttt caaatagtta tctattcata
      241 tgagttttat ttcatgttat ttattgttga caagagcaac tcttgttggt aaagtatcag
      301 tgtcaaaacg taacgattac gatttgagat gtctaaatca aaataaaagg agtataaagg
      361 tatatggcaa aaaagtcgaa aattgctaaa gcaaagaaac aaatggcgat gattgaaaag
      421 tacgcagata aacgtcagga attaaaagcc gcaggagatc gcaccgctct cgctaaattg
      481 ccaagagatt caaatccgaa tcgtctgagg cttcgtgatc agacggatgg tcgtccaaga
      541 gggtatatgc gaaagttcgg tatgtcccgt attaagttta gagaacttgc ccatcaaggg
      601 ttgattcctg gagtaaaaaa agcaagttgg taaaaggagg aaatatcatg gcagtaccag
      661 caagaaaaac atctaaggcg aagaaacggt taagaagaac tcatcaaact gtagtaaaac
      721 cagaaatttc atttgacgaa gcgaatggtg attatagacg tagccatcat gtatctttaa
      781 aagggtacta taatggaaag aaagtaatta aaggagcttc aaaatagttt aatatataac
      841 ttttaggagg gaatcaaaat ggaaattatt tacccacctt tagttgaaga aggattaaag
      901 tattatcttg aaacgaccca gcaatcgcta gataaatgca ctttttatcg ttcgatggta
      961 gagagaggaa ttattactga aacgggttta ccgacccaac aggcaattga aaatggatta
     1021 gtcaaagatt actatgagga ccaaggctta tcatttgatg attttttaag aatctatcca
     1081 atttttgaag agtatgacga ggagttattt caatgtattg atggttattg ggagatacct
     1141 gttgatatga aagaaaattt agtttcacaa ttggaatctg gcgagttaac ttttgaagat
     1201 gcacaacaaa ttcaagcata tcttgaagat cgataagtat tgaaggagga agatttatgc
     1261 gtcaaaatat tattttggaa tgtgtcgaaa ctggagaacg tctgtatctc accagcaaaa
     1321 ataaacggaa taaccctgaa cggttagaat taaaaaagta ttcgccaaaa ttgcgcagac
     1381 gggcaatttt caaagaagtg aaatagctaa gtggtaaaaa ggtgacaaaa tgggcatacc
     1441 agttacaatc gtttctgggt ttttaggggc tggaaaaaca acgctaatca atcaagcctt
     1501 gcaagtctca ccctacccca gggaagaaat tattataatt gaaaatgagt ttggtgaagt
     1561 tgggattgat cacaaattat tagttcatag cgaggaacgg gtgttacaac taaataatgg
     1621 atgtatgtgt tgcagtcttc gcagtgattt attagcaata ttgatttctt tactggaaac
     1681 aatagaagaa catcaacaac caatttcaca aatcatcatt gaaactacag gtatagcaga
     1741 tccccaacca atcattcaaa cacttttaac agcgcctcaa ctaagaggaa gagtatatat
     1801 cgatagttta ttgactgtta ttgatagtga cagttttgaa cttattgaaa gagaaccaca
     1861 agcccttaag caacttgtca tagcagatcg catttttgtg tctagaaaac aaaacttgaa
     1921 tttatctgca ctaaaaaaat tacagagaga aatcaaaagt attaaccctt tatccgatat
     1981 tcgaatgttc acacatagtg aaacgattca aaaaaaggat ttttataatt tggaaaaatt
     2041 caatcatccc ttagtttttg aaagtgattc atcagaaaat cactataatg gcaaacacta
     2101 tcatcaacat catcaagaac atgagtttaa atcaattctc ttaacttcag atcaagacat
     2161 aaaaaaagat tttttattcc aattaattga ccggctgatt ttaaccaacc aagggagcct
     2221 gtatcgtttt aaaggattga ttgctttgca agatcaagat tttatagttg ccatacaagg
     2281 ggtaaacgaa caggttaatt ttcaatacac cactcgacaa aaagaccaaa ccactattat
     2341 tctaattgga aaaatgttgg atgaagaaag gatcaaacag agttttgata aattactggc
     2401 ggaaagcaaa taatgataag tattgattag gggtaatttc taatggagaa aacggatata
     2461 tctagtgcat atcgacgact taaaagcccg aatatcaaaa ctagaaaaag ggcattaaaa
     2521 attatcaaag aacataaaca aaataaaatg aagaaattag cataattaag gaaggaaaaa
     2581 gaatgaagaa aatcagtgta ggacttattg ggatagcagc tctaggatta ttgggagctt
     2641 gctccagcac taatgatgca aaagtatcga atgataaaga tggaaaatta gaaattgtaa
     2701 caacattcta tccaatgtat gatttcacca aaaacatcgt cggcgatgaa gcgaacgtgg
     2761 atttaatggt tccagcggga tcagaacctc atgactatga gccctcagct aaggatatgg
     2821 caaaagctca tgatgctgat gtttttgtat atcataacga aaacatggaa tcatgggtac
     2881 caaaagctaa agaaagttgg aagaaagctg ggcctaatgt agtagaagga acaaaagaca
     2941 tgattttatt gccaggtagt gaagaagaag atcatgatca cggagaagaa gatcaccatc
     3001 atgaactaga tcctcatacg tgggtttcac ccaaaatggc aattaaggaa gtttcaaata
     3061 tcaaggatca gttagttaaa ttgtatccga aaaaagcaaa agtatttgag acaaatgctg
     3121 aaaagtattt gactaaactg aaacggttgg atgctgacta taccactagt cttaaagaag
     3181 cgaaacaaaa aagttttgtg acacaacatg cagcctttgg atatttagct ttggattatg
     3241 gattgataca agttcctatt gctggactaa gtcctgaaga agagccatct tctggtcgat
     3301 tagctgaatt aaaagagtat gttaaaaaaa ataagatcaa ttatatttac tttgaaaaga
     3361 atgctaatga taaaatcgca aaaactttgg ccaatgaagc tgggataaaa ttggaagtat
     3421 tgaatcctct tgaaagtctt acaaaagaac aaatggataa tggtgaagat tatgtttcag
     3481 taatggaaga caatctaaaa gcgttagaga aaacaactat ggttgctggt gaagaggtag
     3541 ttccagaaaa agaagcgaag gatgaaaaga cagttgctaa tgggtacttt aaagatgccg
     3601 atgtgaagga tcgcgaattg agtgattata caggcgaatg gcaatctgtt tatccgttgt
     3661 taaaagatgg aattttggat gaagttttcg attacaaagc aaaactcaat aaagacatga
     3721 cagctgccga gtacaaagat tattatacta ctggctataa gacagatatt gatactatca
     3781 acattaaaga caacaccatt gattttgtag tcaacggtga acatcatcaa tacacataca
     3841 aatatgttgg atacaaaatt ttgaattatg aaaaaggaaa ccgtggtgtt cgttttaatt
     3901 ttgaaactga tgatgcaggt gctggtcgtt ttaaatatat tcaatttagc gatcatggaa
     3961 ttgcaccaag taaggcagag catttccata ttttctttgg tggagaaagt caagaaaaac
     4021 tttataatga aatgcataat tggcctactt tctatccagc ttcattatct gagcatgaga
     4081 ttgctcaaga aatgatggcc cattaaaatc caatctttgg aggaaataca tggtaagaaa
     4141 agtattattg tctatggatt ccaagaatat tgatgcttat cggcgttatc aatttgcaaa
     4201 gcaaatgaga ttaaatggga gtacattagc tgatattgct gcaactttac atgttagtat
     4261 tgacacagtt catagagtat taaaacggga tcctgattgt tgttttatca aggtcaatac
     4321 tggaagaagt agacgtttag caaaacggca taatagcttg gccaattaat tgataatacc
     4381 agagtagatc atttttccct tatttggatt tgatctgctt tttattattt cttgaattgt
     4441 caaatcaagc gcaacacttt atcaaagaag acttcataag gtgttctcca atctaaacac
     4501 tttctaggtc tgttattcag tttttcaatc atctgattta tcgttgagtt atctaccgta
     4561 gctatctctt tcgattttgg caagtattct cgaatgagtc tattcgtatt ttaatttgtt
     4621 cattgttgcc aagggcatga ggatctggaa aataaaaggg catattattc aatacttctg
     4681 taactgcgaa atgattggag aattcttttc ctctatctgg agtgatcgtt ttgaccgcct
     4741 catttggaat actgcttaaa agttcaatca ttttctcgct cacataagcg gctttctttc
     4801 tggggacctt ctttcctgct aaaaggaagc atgattttct gtctgtcaaa gtcactaaac
     4861 aagtaccact agttttgcca gctacaatat cggctttcca atgaccaaaa tcacttctat
     4921 catctgcgga ttttggatgg ccatggatcg aatgagtaag gcaaatcttc ccacgagact
     4981 cgacatgatt ttttgtatgt cttgtttttc ctcgatggtg caatgaacgg atcaggcctt
     5041 tattgccgtg tgagaggggt tctgtatcga aaagatcatt gcaaattgca cggtagatcg
     5101 tgataaaact aattgaaaag tccatatgtt catgcttcaa acgattcgag attttctctg
     5161 gcgaccactg aaaatcgaca aataaatgac gaacaattga ccaaactcac ggatcattta
     5221 gaatgtgata acgtccgcat ttttttcttc gataagcata atttttttcc gctttggaag
     5281 gtgattagtt tttttcttgt ttcttctcag ttcatgtaaa atagtaaacg ggctatgctg
     5341 aatgtcgttg ccaattatac taatagaaaa tccttcttcg tgaagcagaa agattctttc
     5401 tcgtttgagt atagtaagat gacaatactg gctcataatt attttccttc taggagtgtt
     5461 ctcgacaaac ttatactagt tggatatcga ttatgactca tttttatgtg ttgcacttaa
     5521 agtgtaaatt caaggtatag aaagaggttc cctatgaaaa aatacctatt acaatcttaa
     5581 ctggctatct tggttcagga aaaacgatac ttctaaatca tattctgtac ggtgatcacg
     5641 gaaaggaaat tgctgtcatt gtaaatgaca taggggaagt aaatgttgat ggggtattag
     5701 tcgacaagaa aggttttaaa agaactggag aaaaacttgt ttcgatgccg aatggatgca
     5761 tttgttgtac attacgcgaa gatttgattg ttgaactaga tcaattagtg caattttctg
     5821 acttgaatca aattgttatt gaagcttcag gaatcagcaa accaattcca attattcaag
     5881 caattatatt agctaaaaac cctttagaca ttgatttgac ccaacgatta gagattggtg
     5941 ccattattat agtagtggat gtaaaacgtt tttgggataa tttcgaaacg ggtgaaaaga
     6001 ttcaggaaag aaaagctgaa ggtaatgtag ttgaagaaga acaggatatt cgtggcttgt
     6061 taattgatca attcgagtgt tgtaacatat tacttttaaa taatagtgac ttagtgtcaa
     6121 ccaagacgat ttacaaaatt aacggtctaa ttgctaagtt acagtccaat gccaaaataa
     6181 ttaggacaat taaaggtgaa gtagacttat cgaaaattct aaatacaaat ctttttaata
     6241 tagatgagac ttcctattca atgggctggt tcaaagagtt agagcttggc tatcaaaatc
     6301 atattcctga aacagaagaa tatggtatct caagttttat ctatcgaaat cggttactcc
     6361 ttgacgcaca aaaatttgcc catcttacgg ataaaaattt tcctggcaat attacaagag
     6421 caaaagggtt tctttggttt gcccaacata gtgatttttc gacgatcttt gaaacatcgg
     6481 gtagccataa aagtattgaa tcattaaatt attgggtagc tagtttacct aaaattgagt
     6541 gtcaaatgat tatacaaaaa aacccgaatt gaaggaagcc tggcatccgt tgtttggtga
     6601 acgcattaat aaactagttt ttattggtat gaagatggat caacaatgta ttaaggaaca
     6661 acttgagggt tgtttaactg attctcttaa tgcagataat ttccgaaaaa tttcaccatt
     6721 tccatggaaa aattctaata tgtttagcca gatatcgtta atgaaatttt taagaaaagc
     6781 tggtgtcaag ataggcgatg tcataattat tggcattttg attattccct cttttattcc
     6841 cttatttgta ttctctatag aaaaggagaa tgaaacaact gaagatgcta tcaaatatgc
     6901 catcgttcaa attgatggca aggaaatgga tcgcttcgat ttggataaga tcaatcataa
     6961 actcgtgaat tatcatcctg cgaagggaca atataatatt gttgagatta aagatggaag
     7021 aattcgagtt aaagaagata atagtcctga tcaaatcgcg gttagaactg gatggatctc
     7081 aaaacccggt caaacgagta tttgtttacc ccataagtta gttattagta ttgagaaaaa
     7141 agaaagcaaa gattactaca tttactaatc tggaaataga tgaggaggtt tttaataaga
     7201 aaaagatatt attattaata atagcagggg ctttattaag tgtgttaagc gcttgtggac
     7261 aagaaaaaga agcggtgaca acaggcacaa gtatctttaa cgggacctgc aatggtttag
     7321 ttaacagtcg gttagattac ttattttggc tcttcgtcaa tgagtgttgg tgaagaaatt
     7381 caatagaata atcgagatac tcacacggct agacagctat cttgtctagc cgtgttgttg
     7441 ttctggcttt agtcagttgg gtagtgttct tagttttctt gaaatatttt cctcgatata
     7501 aaaagattcg ttctcggtag ttttgaaagt ttctaaagcc ataggcaatt cgttttagta
     7561 ctttgatatg gttgttcaaa cattctatag ggccgttgga atagggaact cgcaacgctc
     7621 gtttgatttc tgttcgatat ttcttgaagg tcgtgaacac tggttgatag tgctctggca
     7681 actccttgta gtcttctttt aggagcgcga cgaattcagg aacatcttgt cttctgatag
     7741 cggaaagaaa aacctggtag acttcgtagc ctcgctttaa ggagtcatca taatataata
     7801 gccgatcgac tatctccgct tctgtcagat gatctcgaaa tgacggacgc cagtagcgat
     7861 gctgaccatt caattcccac gcattctttt gaagtagtct ccagtatttc tttaagtgct
     7921 tcgaccgacg atcccctttt ttaaaggtct tcattacttg aattcggtgg ttggtaaagg
     7981 cccgaccgat gtgttggcta atatgaaagc gatcggcgac gattcgggca ttcggaaaac
     8041 actctttcac caatgtgcga tagggtgtat agaaatctga aaccactaga cgtacttgtt
     8101 cacggacttt tctagggtag cgcaagaaat agtcgcgtaa aggattgagt tgccgatttt
     8161 ccacaatatc aatcagttga tgggtcttgc cgtccatcat gacaaagctc atggctcccg
     8221 agacgttttt caccgatttg aattcatcga aacacaccac ttctggtaac ggctggttca
     8281 aagaaacggt tttaggccga aggctgcgaa gtattcgaat gacagtggtc ggagaaatgt
     8341 gcttcatacg tgcaatcaaa gacatagaaa taggctcact taatagctca agaatggctt
     8401 gtttgactct gcgggcaatg gagcagcgac gatcgctaac tgaatcttct gctaaaaaag
     8461 tctttccaca gtgtttacat ttgaagcgct gtttgttaat ccgcacaatg gtcttgtagt
     8521 tactgacatc attcaataag atggatactt ttttccagcc ccatttaatg atcgtttgct
     8581 tattttgata gtggcaatgg aagcattcct ctggttgata ggtcaatgtg ccatcaaaaa
     8641 caaaacatgt ttcgtcatta atgcgctctt tgcgaaaggc atcttcatga aaggtaagat
     8701 ttaggtctag aatatctagt aactctttgg taaactctga ataggacatc ttgaaacact
     8761 cctttgaatg gttttcgtcg actttattct aaaggatttc aaggtgtctg tttgcgttta
     8821 ttagaaaaaa gaaaaagtat tggcaacaga aggcgtcacc aacactagat attatagatc
     8881 ccttattttc ttcgcttcaa attgtcaagg agtcatcata aattttgtat ggattctttt
     8941 gtttgggtag tagtcttcta aataatcttg gattaacacc ttcatctcta ttttactttt
     9001 atatttgttg gagtcgatta attctcattt gattgatcga tgaaacgctt caattactgc
     9061 attgtcaaca ggagtcccag tttggctcat tgaatgtgtg atgtggtggt cggtcaacag
     9121 ctcttgataa ataaaagacc gatgctggct acctctatcg gaatgaatta ccgtaggcaa
     9181 ttctccttaa taaatttgta atggacggac gacaatctct tttttcatgt gtgaatccac
     9241 catatagtaa cgtagaatac gagttgctaa gtcaatatag gtggacaaat agaggcgacc
     9301 gtcattacat ggaatgtaag taatatccgt cacccaaaca gcatcaattt ggttctgttc
     9361 aaattatcga ttcaacattt tttttaccat ttctttgatt gttttttctc taccatgaga
     9421 agatttgcgt cgatgaaatc ctcttgtgca taaattcatt tcacgcataa gaacagctac
     9481 aacacgttta ttgatttcga ttccttcatc gtaaagtttt tgagtgatcc ctggagatcc
     9541 gtatcgatct ttgtgttcgt agaacactcg tcttcctctt ttagtcgttt attttcctgt
     9601 tcaagaatgt agaaatctgc tttatcaggt aatctttttc cttgtccagc aaaagcattt
     9661 ttcccgtatc gacgatattc tgaaacccat cgggaaattg ttggacgaga gatattgtat
     9721 tcttcagcaa ttttcttggt agttactttt ttctcaagga tatcctgtga aatctttagt
     9781 ttaaatttac gatcatatgt ttttttcaat agagttcatt ccttccggta acgtaactga
     9841 tagtcaagaa aagtgtatca tagcccaact tagtatcagg gtttgtcaac taaactgtgg
     9901 aagttgatag ataagagttt ttaacaacca aaattctctt gactattttt gaaatcagac
     9961 aaattttgat tggacacata gttgaataaa tctaccgtcg ttacggaagg taaatcgcat
    10021 gcttttcaat tctagaggag aagtcctcgt tgaagtacca tggcccagtt ctggattcga
    10081 cctcctgccc acttcgtacg taaataaagt aattttagga gtgagactgt gacaaaagtt
    10141 atttttaagc taaaaacgaa ttaaaaaacg ttaataatta aaatcattcg ttttagattc
    10201 tatgaatcat tatagaacgc tgtaaaatcg atactttata attataaaga agaccgaagg
    10261 tattcattaa ttctttaacc aggactaacc ccgaatcact tgatagacga ccaccggtat
    10321 gtgaaatagt gatatttgaa ttgaatttaa cttgattttt gtgtaagcta gtcatgagaa
    10381 gaactccttt cttatggttg gtttcgtcac ctttaccata gcagaatggg gttctttttt
    10441 catcacttaa ggggtgaaac taaaaaagta attatcagac tgccgttagg cagtttcaga
    10501 cggtttcaat taaaaagtat gaattattca ggagaagatg gcttcaaatt tattgttcga
    10561 aatttgaaac tatcttttta cttatgtcac agactccctt agttgagtta ttctttaatt
    10621 tcgaatttcg agatagctag atatattgca agcatacatt cttgataaca atcttgatca
    10681 tattttccag ttctccagga ttgcttatgg accaaagcgc taaatctttt gagaatggcc
    10741 acaactgctt cttcatttcc attctttgct aaaatgaaat cttcttttaa agtattcata
    10801 aatttacctt cttcccatga acttataatc tatttatttt tttgggaagg aggaggacga
    10861 gcaaagaagc atacaaaatt agttattatg cagatattaa accagcattt tttgaatttt
    10921 tttgtaaagt cgatgtttta aatttgtcgc accctgtctt gtaataccca gaacctttcc
    10981 aatttcttta tcagttttat taaagataaa tttttcgata agaaaaattt gttcaggttc
    11041 tttcaacaaa ggaaggattt ttgctaattt tatatcttca acaaggagag tcacatttaa
    11101 aacattaatc ggttgtttaa attttatagt atctttagta aacaaatatg gtgggagttg
    11161 atcaagttgt tcatgttttt gaatttcttc ttgtttttta taataagtat tagcggtgtt
    11221 caaaatgacc ttttcaaaga atcgaatcaa ctgggcattt acgcgttcgt cgttatctga
    11281 catattgcac acctcctcca ttaagattta aagggaaaag aggcaaaaaa cacgaaaaga
    11341 agctaatgca attatgctgc gctgcgaata acttctataa gctcacatgc attgatagga
    11401 ctgctgatgt ttattttcaa aaatgttttt ttagttgata atggatagtt atctttatac
    11461 ttagaaaaga aaattcatat atcttcttaa atatcaaaca tgtccttatg gctaagaaaa
    11521 tgtcttgagc tttttacgta taataaatct tttctaaaac ctccttttgg ataatattct
    11581 tattcgtgta tagattgtat tttttatttc ttcccccttc ataattaaca actagaaaag
    11641 agttcgaata gacacgtatt tgaacgaaaa tttttattgg attcccaaat tggagtattt
    11701 agaaacgaaa aattcctatt atctgtatag tgcttagata ataacggatc ataataatta
    11761 ttgtctttga actagatata tgtaaatagt attcatcatg atgaaaaatg atgagttctc
    11821 ggtgataaaa tgtacttttt acggtggaat atacatgaaa gcgataagtg gatattttat
    11881 aatgaagtaa gttagcttat tttgaca
//
LOCUS       DAKBVG010000076        11751 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000076 DAKBVG010000000
VERSION     DAKBVG010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11751
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.076"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            42..197
                     /locus_tag="OUD09_001443"
     CDS             42..197
                     /locus_tag="OUD09_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002392227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9692717.1"
                     /translation="MVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGVKDVLLNL
                     FNRIVGA"
     gene            210..713
                     /locus_tag="OUD09_001444"
     CDS             210..713
                     /locus_tag="OUD09_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HCT9692718.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKEQIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCNLAEELEGTPIGEAASEIQHAFFNSFEEMVEH
                     VDDIVYYPDCDDMSDVAHYLIVEAGDFGEVPEQLKPYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPE"
     gene            840..1877
                     /locus_tag="OUD09_001445"
     CDS             840..1877
                     /locus_tag="OUD09_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9692719.1"
                     /translation="MANIQTSFIDFHNSIRLDVEDNTLLKDYKDQVIDGLKDYLPDDV
                     KFETFLQGSYSVYTGIKSCDEKIDFDIDIAVAFEIDHTVYEDPREPKLWVKEALVEIF
                     PNAQVNLKVPCVTATFTGKKTKKNVHVDVAVYAKEDENYFLAKAKEFSAPENRCWEEA
                     DPKVLKEKINSHVADSDDRKQFRRCIRYLKRWKDNNFNQEYKPTGIGLTINVMDTFLV
                     NKSTDFLTRKVQYNDMECMKQIVSSLKDSFVYEYSETDGWHYRLHAYLPVKPNSDTYS
                     KMTVNQMSDFKNKLSKLYDDLIFAIDTEDEYEATKRLNNQFGEDFLIISEEEVTEKNL
                     RNAFVTDYPSA"
     gene            1892..3589
                     /locus_tag="OUD09_001446"
     CDS             1892..3589
                     /locus_tag="OUD09_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HCT9692720.1"
                     /translation="MDYTDILKEKLTDVVRLFDGNYYGDFSFATIGKLRLKIEIPKHF
                     PLVLPRIYIHNFDHLGLYLPHVEKDGLVCFSSDINYLVDEENSELVLLQCVNKTIKTI
                     EEGLKGLNKNDFRKEFVAFWDKQENQISLFYLCEPALFIKSVDTIMYGKSLVALDKEN
                     ELQLNAASRLFNGEKEDTHELDAYYVPIRNKNNIMPPKNNKAWSKKDILQIVKKNVTG
                     ANKRFFLKLEKRKNRHNVLLILDIPIEEKSNNVIIGFLFTKQSGSIKDYKGTPRTVLV
                     QRHDELYLVNRTSGENYGLGANIAIVGVGSVGSKIAENLGPLGVRKMTLIDNDCFQAE
                     NLYRNSLGADSLKNARSQNNFFKVDLMQEELEKKFPYLCVEVESMNVLDLISMQPGFF
                     KEYDYVFVCIGDTMASLALNNYFSKSSQKVFYSWLEPYGIGGHTLFINYSLNQKGCFN
                     CLYTDSNSKNRCSNRASLVKEGQIFERTLASCRSKFIPYNAIASTECAINAIKLFHKV
                     NQNNMTNSTLFSWIGDGSTFENLGYQYSDRNTFLNGVNKFEERFFSKYCKVCGELDD"
     gene            3582..4043
                     /locus_tag="OUD09_001447"
     CDS             3582..4043
                     /locus_tag="OUD09_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HCT9692721.1"
                     /translation="MIKFSCDRIVIEVRDEVLKIFEQYRQGKDDLPEAGGVLHGKRLK
                     NGSIIITDITTPQIGDIQKRLLFKKNREIHQQLSNEKWIESNKFSIALGEWHTHPEAV
                     PTPSNVDKKSWRLNVSKQHDDRVYVFIIVGFSDLRMWLLSKKSDIRQAIKL"
     gene            4056..4916
                     /locus_tag="OUD09_001448"
     CDS             4056..4916
                     /locus_tag="OUD09_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9692722.1"
                     /translation="MKSQKNIWWWGFLVGKVMIPALLLISLPMITFFFTNKKMSKEAV
                     IFFFGDQKATFIETLVTSLQLNLNYIFSIIIVISLLNFFKKRNSGKVFNSNGNVYYNY
                     FYFVFWVAATLLGYDKIQIAGIPIHMQYKLVLSGIFSEVLPDIYDDHYDSDGTCKVSI
                     EKENFDDIDGYDSVNLLIIDTYDIKMSELSMENQTYPTIIVRGNSIDGVRKVNRSLIL
                     EIKKTMDEIQKSDFKKVFVASTSNPKNSINIINSSFRFFGRSRRFKLYVLQKDYASNG
                     KYSKKYRIFI"
     gene            4954..5343
                     /locus_tag="OUD09_001449"
     CDS             4954..5343
                     /locus_tag="OUD09_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCT9692723.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLMFV
                     IVFAHVPPLSMIEGAFLKYLGIPIAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            5327..7777
                     /locus_tag="OUD09_001450"
     CDS             5327..7777
                     /locus_tag="OUD09_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCT9692724.1"
                     /translation="MMYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKYQVHD
                     NFRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDLQTDALVSM
                     IGDSQIDYRFFIGFKLISTDEEVNLKNLRKSFFSGLQEFVYGVNHHLMGDFVSLSNEE
                     IRRYTKLEKLMESKLARRFKVKRVTPSDLTYLIEHIYGEKGTPFEEYEFQLPKKKLKS
                     ETLVKRYDLLRPTRCLIEEKPRYLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQ
                     QQQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSV
                     DELETTLDQSKESMYKLSYVVRVSAKSVDELKRRCDEVLDFYDDTNVKLVRPFGDMMG
                     LHKEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKP
                     ALASQGVKGSVTNALASAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWK
                     ETLPEIADEINILNLTSKPENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEK
                     FPVLRKAIRTVTQSDQRGLLQVIVELRKEDSPVAENIADHIESFTDYDFAHLLFSDGS
                     ITQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNRE
                     IFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGL
                     KFAFRSTDIHEIKKTLEFFGLDKEDESNQKCMRDLENGQCLMQDLYGRVGVVQVHPVF
                     EELFHAFDTRPPVQERME"
     gene            7782..9890
                     /locus_tag="OUD09_001451"
     CDS             7782..9890
                     /locus_tag="OUD09_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCT9692725.1"
                     /translation="MKKKLFRIFGIVLISLVTLIILLSLVGTVAEATGLVDDTVGAGN
                     LYSQYSLGNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEVGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMNMFNLQSNDSQSMGRRVMRKPQMLMNRTLR
                     QLNRNVGRTLAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEIPSDLNKEK
                     PIPNNKKQSRMNLAGRKVGKVLDTQALVKDKTKQVKDGVRNTPTNLKYNFHKGIEKTK
                     KAPEEFKRGLVQEKANRGELREKQQQRRDEKIAEKRKVLNEIGNPHGKRRENVPIKAK
                     VQSQKDRAPKRIVRANSNPEIKRKFASQEIISKGINQSSSKKNSFQQVKRRSTLSKRT
                     NRKVQKSLNPHPKPRSGEKK"
     gene            9887..10891
                     /locus_tag="OUD09_001452"
     CDS             9887..10891
                     /locus_tag="OUD09_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HCT9692726.1"
                     /translation="MNVKKLSAISLTFLTISFMGILMCVGLLFGEDSEGSSSSGAPPG
                     GSSVSEEVLKHRAMVEKYAKESDITEYVPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNFGGGYLDYVAKNGKKHSFALA
                     ESFSKEKSCGQKIDYPNPIANKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSRPETGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTESYWQQHLIGAGRYK
                     "
     gene            10910..>11751
                     /locus_tag="OUD09_001453"
     CDS             10910..>11751
                     /locus_tag="OUD09_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCT9692727.1"
                     /translation="MKIKIERNQKEKSLKKKKERVVSVGKHRKMVLALWLLLSCSLTF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGIENFTKDFSKEYFSWKNNKEAIEKRMNNL
                     EQYLTEEGFALSQDMVRADIPTNSEVQSVKVLDVEKSSEEFVVSFLVEQKITEGKKAQ
                     SISSAYRVTIFEDENRNYIVTSLPTMIAKPDRAKYKTKQVENDSKIDAKTTEEIAEFL
                     ETFFKLYPSASEKELEYYVENDAMRPINIDLKFSKLEDPVYLQNGKKILVSTKIKYLD
                     NVSKT"
BASE COUNT         3914 a         1774 c         2508 g         3555 t
ORIGIN      
        1 aatggttttt aaccaatgct caatctttag tcttactagc gattgtggtc attggtttgt
       61 ttttaggatt taaacgggag ttttccaaac tgattggctt cttggtgatt gctttgattg
      121 cggtaggtct ggtctttaat gcttctggtg tgaaggatgt cctactcaat ttgtttaacc
      181 gaattgtcgg tgcctgaggg ggaatcacaa tggaacagat gcgtgtttat attgcgaacc
      241 ttggaaaata caatgaaggt gagttggtgg gggcatggtt taccccacct gtcgattttg
      301 atgaagtgaa agaacagatt ggattaaatg acgaatatga agagtacgcg attcatgatt
      361 atgaactgcc ttttgagatt gatgagtata cgccaattga agagattaat cggttgtgta
      421 acttagcgga ggaactagaa ggaacgccaa ttggtgaagc tgcttctgag attcaacacg
      481 ctttcttcaa ttctttcgag gaaatggtag agcatgtgga tgatattgtc tattatccag
      541 attgcgatga tatgagtgat gtggcgcatt acttgattgt agaagctggt gattttggcg
      601 aagttccaga acaattgaag ccgtatattg attttgatgc ttgcggtcga gatttggaga
      661 ttggaggaaa ttatttggtt actagtcgtg gagtttttga gtatccagaa tgaatcaaac
      721 actatatata gtgtttgatt agtatatgta agcaaaaaaa cacaatatat agagtgcaaa
      781 ctgaagggta atgtgatata atatttgccg aagaaatatt tttgaaatga ggtaatatta
      841 tggcaaatat tcaaacaagt tttattgatt tccataattc aattagattg gacgtagaag
      901 acaatacgct acttaaggac tataaagacc aagtaattga tggattaaaa gattacttac
      961 ctgatgatgt gaagtttgaa acttttttac agggaagcta ttctgtttat accggaataa
     1021 aaagctgtga tgaaaaaatt gattttgaca tcgatattgc agttgctttc gaaattgatc
     1081 atactgttta tgaagatcca agggagccta aactgtgggt aaaagaggcg ctagttgaaa
     1141 tttttcctaa tgctcaagtt aatttaaaag taccttgtgt aactgctaca tttactggaa
     1201 aaaagacaaa aaaaaatgtg cacgttgatg ttgcggttta tgctaaagaa gatgaaaatt
     1261 atttcttagc aaaagcaaaa gaattcagtg ctcccgaaaa ccgatgctgg gaggaagcgg
     1321 atcccaaagt attaaaagag aaaattaata gtcatgtagc agattcagat gatcgaaaac
     1381 aatttcgaag atgtattcgc tatttaaaaa gatggaaaga taacaatttt aaccaagaat
     1441 ataaaccaac aggaattgga cttacaatta atgtgatgga tacgttccta gttaataagt
     1501 ccactgattt tttaactaga aaagttcagt ataatgatat ggaatgtatg aagcagattg
     1561 tttcttcgct gaaagattca tttgtgtatg agtacagtga aacagatggt tggcattatc
     1621 gtctacatgc atatcttcca gtgaagccta acagtgacac ctattcaaaa atgacagtga
     1681 atcagatgag tgattttaaa aacaaacttt ctaaattata tgatgatttg attttcgcta
     1741 tcgatactga agatgagtat gaagctacaa aacgattaaa taatcaattt ggggaggatt
     1801 ttttaattat ttcagaagaa gaagttacag aaaaaaactt aagaaatgca tttgttacag
     1861 actatccgag tgcttaggtt ggtgttaaag attggattat actgatattt taaaagaaaa
     1921 gctaacagat gttgtgcgtt tattcgatgg taattactat ggggattttt catttgctac
     1981 aattggaaag ttgcgtttaa aaattgaaat accaaaacat tttccattgg tacttcctag
     2041 gatttacatt cataattttg atcatttagg actttaccta ccacatgtag aaaaagatgg
     2101 gttggtatgt ttttcttcag atataaatta tttagtagat gaagaaaatt cagaattggt
     2161 tttattgcag tgtgttaata aaactataaa aacaattgag gaaggtttga aagggttaaa
     2221 taaaaatgat tttcgaaaag agtttgttgc tttttgggac aaacaagaga atcaaatttc
     2281 gttattctac ctttgtgaac cagcgttgtt tatcaagtca gttgatacaa taatgtacgg
     2341 taagtctttg gttgctttgg ataaagagaa cgaattacaa ttaaatgcag cgagtagatt
     2401 gtttaatgga gagaaagaag atactcatga attagatgca tactatgttc caattagaaa
     2461 caagaataat attatgcccc cgaagaataa taaagcatgg tcaaagaagg atattttaca
     2521 aatagtaaaa aaaaatgtaa caggtgcaaa taaaagattt tttttaaaac ttgaaaaaag
     2581 aaagaataga cataatgttt tacttattct agatatccca attgaagaaa agtcaaataa
     2641 tgtcataatt ggatttcttt ttacaaaaca atctggatct ataaaagatt ataagggtac
     2701 gcctaggact gtattagttc agcgtcatga tgaattatat ttggtgaata gaacgagcgg
     2761 agaaaactat ggattgggtg ctaatatagc tattgttggg gtagggtcag ttggcagtaa
     2821 aattgctgaa aatcttggtc cattaggtgt gagaaagatg acactcatag acaatgattg
     2881 ttttcaagct gaaaatttgt atcgaaattc acttggtgca gatagtttaa aaaatgcccg
     2941 atctcagaat aattttttta aagttgattt aatgcaggag gagctcgaaa aaaaattccc
     3001 atacctttgt gttgaagtgg agtcaatgaa cgttttagac ttaattagta tgcaacctgg
     3061 ttttttcaag gaatatgact atgtttttgt ttgcatagga gatacaatgg caagcctggc
     3121 tttaaataat tatttttcca aaagtagtca aaaagttttc tattcttggc tggagccata
     3181 tggcattggg ggacacactc tatttataaa ttattctcta aatcaaaaag gttgtttcaa
     3241 ttgtctttat acagattcaa actctaaaaa taggtgttca aatagagctt ctctggtaaa
     3301 agaaggacaa atttttgaaa ggactttagc gagttgccgc tcaaaattta taccatataa
     3361 tgctattgcc tcgacggaat gtgcaattaa tgcaataaaa cttttccata aagtcaatca
     3421 aaataatatg accaatagta ctcttttttc atggatagga gatgggagta cttttgagaa
     3481 tttaggatat caatatagcg acagaaatac ttttcttaat ggagtcaata agtttgaaga
     3541 aagattcttt tctaaatatt gcaaagtatg tggggagtta gatgattaaa ttttcgtgtg
     3601 accgtattgt cattgaagta agggatgaag ttcttaagat atttgaacaa tatcggcagg
     3661 gaaaggatga cttgcctgaa gctggaggag ttcttcatgg aaaaagactt aagaatggga
     3721 gcataataat taccgatata acaacacctc aaataggaga tatacaaaaa agattactct
     3781 ttaaaaaaaa ccgagaaatt catcaacagc tttctaatga gaaatggata gaatcaaata
     3841 agttttctat tgcattggga gaatggcata ctcatcctga agctgtacca actccatcaa
     3901 atgttgataa gaaaagctgg agactgaacg tgagcaagca acatgatgat agagtttatg
     3961 tttttattat cgttggattt tctgatttga gaatgtggtt actgtcaaaa aaatcagata
     4021 ttaggcaggc tataaaacta taaaggagtg attaggtgaa atcacaaaag aatatttggt
     4081 ggtgggggtt tttagtagga aaggtaatga taccagccct tctcttaatt tctctaccga
     4141 tgattacttt tttctttacc aataaaaaaa tgagtaaaga agccgtgatt tttttctttg
     4201 gagaccaaaa agctaccttt attgaaacgt tagtaacatc tcttcagctt aatttgaatt
     4261 acattttttc aattattatt gttatatcgt tattgaattt ttttaaaaaa agaaattcag
     4321 gaaaggtgtt taactctaat ggaaatgtgt actacaacta tttttatttt gtattttggg
     4381 tagcagcaac tttacttggt tatgataaaa ttcagattgc aggaattcct atacacatgc
     4441 aatataagtt ggttttgagt ggaatttttt ctgaggtgtt gccagatata tacgatgatc
     4501 attacgacag tgatggaaca tgtaaagtaa gcattgaaaa agaaaatttc gatgacatcg
     4561 atggatatga ctctgtgaac ttactgatta ttgatactta cgatataaaa atgagtgaat
     4621 tatctatgga aaatcaaaca tatcctacga taattgttag agggaattca atagatggag
     4681 ttcgtaaagt taatagatct ttaatcttgg aaataaaaaa aacaatggat gaaattcaaa
     4741 aaagtgactt taaaaaagta tttgttgctt caacatctaa tccaaaaaat agtatcaata
     4801 ttattaattc atcgtttaga tttttcggtc gatcacgtag gtttaaactt tatgttcttc
     4861 aaaaggatta tgcaagcaat ggaaagtata gtaagaaata tagaattttt atttaagaag
     4921 gaatttttcc ttctttttat tttggaggaa actatgaaaa aaataaaaag ctacaccagt
     4981 atttgggcag ttgaaaaagt catttatgcg attaatgact ttcagctgcc ttttcctgtg
     5041 acctttaacc agatggcttg gtttgtttta tccttgatgt ttgtcattgt atttgcccat
     5101 gtgcctccgc tatccatgat agagggggct tttttaaaat acttgggcat tccgatagcg
     5161 gtcacttggt tcatgtcgca aaagaccttt gacggtaaga aaccattggg atttttacga
     5221 tcctttatca gctatcactt acggtttaag gtcacttttg caggaaagaa agttaaagaa
     5281 cagaagaaac gatgggacga accaatcacc ttggtaagga gtgtgaatta tgtacccgat
     5341 taagtatatt gaaaataatc ttgtctttaa ccaagaaggc gagtgttttg cttactatga
     5401 attagtgcct tacaattact cctttttgtc acctgaacag aagtatcaag tacatgataa
     5461 ctttcgccaa ttaattgcgc aaaatcgtga aggtaagatc catgccttac aaattgccac
     5521 agaaagtagt attcgtgcca cacaagaacg ttcgaaaaag gaaattaccg gtagactcaa
     5581 agaagtagca aagcaacgaa ttgatttaca gacagatgca ttagtatcca tgattggcga
     5641 tagtcagatt gattatcgct tctttattgg ctttaaactg atttcgacag atgaagaggt
     5701 caatctaaag aatctcagga aatctttctt ttctggactt caagaatttg tctatggagt
     5761 gaatcatcat ttgatgggtg attttgtttc cttatccaat gaagaaattc gtcgctatac
     5821 caagcttgaa aaactaatgg aaagtaaatt ggctcgaaga tttaaagtca aacgagtaac
     5881 gccgagtgac ttgacgtatt taattgaaca tatctatggt gaaaaaggca caccgtttga
     5941 agaatatgaa ttccagctac caaagaaaaa gctgaaatca gaaacgctgg tgaaacgtta
     6001 cgacttatta cgtcccactc gttgtttgat tgaagaaaag cctcggtact tacggatgga
     6061 acatgaaaat catgagtctt atgtagcgta cttaacaatt aataccattg taggggaaat
     6121 ggagtttcct tcctcggaac ttttctatta ccagcaacaa caatttacgt ttccgattga
     6181 tacttcaatg aatgtggaaa ttgtgacgaa taaaaaggcc ctagctaccg ttcgtaataa
     6241 gaaaaaggaa ttaaaagatt tagacaacca tgcctatcaa tctgataatg aaacgaactc
     6301 caatgtatta gatgctttgg attccgtgga tgaattggaa accaccttgg atcagtcgaa
     6361 agaatccatg tataaactaa gctatgtggt tcgagtgagt gctaaatcag tggatgaatt
     6421 aaaacgccgc tgtgatgaag tgctcgattt ttatgacgat accaatgtaa aactagtccg
     6481 gccctttggc gatatgatgg gtctacacaa agaattctta ccctcaagta agcgatacat
     6541 gaatgactat attcaatatg ttacgtctga ctttttggcg ggacttggtt ttggggctac
     6601 tcaaatgcta ggagaattag aaggcattta ctttggctat aacgtagata cggggcgcaa
     6661 tgtttacctg aaaccggcat tggcatcaca aggggtcaaa ggctcagtaa ccaatgcttt
     6721 ggcatctgct ttccttggtt cgttaggtgg ggggaaatct tttagcaaca atctcttagt
     6781 ctattatgcc gtcctttttg gtgggcaagc tgtgattgtc gatccaaaag gagaaagagg
     6841 cggttggaaa gaaacgttgc cagaaattgc ggatgaaatt aatattctta acttgaccag
     6901 taagccagaa aatcaaggct tacttgatcc ctatgtgatt atgaagaaaa agaaagactc
     6961 agaaagtttg gcgattgata tcctgacctt tttaacaggg atttccagtc gggatgggga
     7021 gaagttccct gtgttacgca aagccattcg aacggttacc caaagtgatc agcgaggatt
     7081 attacaagta atcgttgaat tgcgtaaaga ggactcacca gtcgcggaaa atatcgcgga
     7141 tcacatcgag tcctttaccg attatgattt tgcccatttg cttttctcag atggttcgat
     7201 tacccagtcg attagtttag ataagcagtt aaatatcatt caagtagcgg atttggtttt
     7261 accagatgca gaaaccactt ttgaagaata taccaccatg gaattgctga gtgtttccat
     7321 gctaattgtt atttccactt ttgccttgga ctttatccat agcaaccgag aaattttcaa
     7381 gattgtggac ctggatgagg cttggagttt tcttcaagta gctcaaggaa aaacgttatc
     7441 gaataaatta gtgcgtgctg gtcgggccat gaatgccggt gtttacttcg tcacgcaaaa
     7501 cagtgatgac ttgttggatg aacgtttgaa gaataatatt gggctaaagt ttgcatttcg
     7561 cagtaccgat attcatgaaa tcaagaaaac cttggaattc tttggattag ataaagaaga
     7621 tgaaagtaat caaaaatgca tgcgagattt ggaaaatggg caatgcttga tgcaggattt
     7681 gtatggtcga gtaggtgtgg tccaagttca tccagtcttt gaagaacttt tccatgcttt
     7741 tgatacccgt ccgccagttc aagagagaat ggagtgagga gatgaaaaag aaactttttc
     7801 gtatctttgg aattgtttta atcagtttgg tcactctgat aatacttttg agcttagttg
     7861 gaacggtagc tgaggcaaca ggtctggttg atgatacagt tggagccgga aatctttatt
     7921 cccaatattc acttggtaac tatcagttgg atttctttgt ggatagttcg tgggattggc
     7981 tgccttggaa ttggggtgat ggcctaggga agagcgtaat gtatggactt tatgccatta
     8041 ctaattttat ttggacagtt agtctctact tatcaaatgc cacgggctac gtggttcaag
     8101 aagcctataa gctggatttc atttcagata cggctgaaag tattgggaag aatatccaaa
     8161 ccttagccgg cattaccgaa aatggactcc agacctcggg cttttacttt gggtttctat
     8221 tattgatgat tttagctcta ggagtttatg tggcgtatac aggattatta aaacgagaaa
     8281 ctaccaaagc agtccgggct gtcattaatt ttgtgatgat ttttctactt tcgggttcgt
     8341 tcattgcgta tgcgcccacc tatatcacaa aaatcaatga ttttagctca gatgtcagcg
     8401 aagcggctct tagccttgga accgagattg tcgtgccgaa ttcagaaagc caaggaaagg
     8461 acagtgtgga tttaatccga gatagcttgt tttccattca agtccaacaa ccgtggttac
     8521 tcttgcaatt tgatgattcc aatatagaag aagttggcga agatcgtgtt aacaaaatcc
     8581 tgtctgtaag tccagatgaa aataaaggca aggatcggga agaagcggtc aaagcggaga
     8641 ttgaagataa tgacaatgcg aatctcagta ttaccaagac catgaaccgc ttagggaccg
     8701 tggtcttttt ggtgttgttc aatattggca tatccttctt tgtctttctc ctaacaggga
     8761 ttatgttgtt ctcgcaaatt ctctttatta tctttgcgat gtttttgccg attagtttct
     8821 tattgtccat gttgccgact tatgagagtt tgggcaagaa ggcgattatt cgcttgttca
     8881 atacgattat gatgcgagca ggagttactt tggtgattac gactgcattt agcatctcca
     8941 cgatgttttt caatatttcg gcaacctatc catttttcat ggttgctttt ctgcaaattg
     9001 tgacctttgc cggtatctat ttcaaattgg gcgacattat gaatatgttc aatctccaga
     9061 gtaatgatag ccaatcaatg ggaagacggg tcatgcgaaa accgcaaatg ttaatgaatc
     9121 gaacattgcg gcaactcaat cgaaatgtgg gacggacttt ggcttttggt ggtggtgcag
     9181 cagttggaaa taagttggca aaggaacaat caaaatccaa atttaagcca tccggttctt
     9241 ctctacgaaa gaattcacga ttaccgaacg atcacgagat accatctgac ttgaataagg
     9301 aaaagccaat tccaaataat aagaaacaat cccgaatgaa tttggccggg agaaaagtcg
     9361 gcaaggttct ggatacccaa gctttggtga aagataagac caaacaagta aaggatggag
     9421 tacggaatac gccaaccaac ttgaaataca acttccataa aggaattgaa aaaacgaaaa
     9481 aagcaccgga agagtttaag cgaggactgg tccaagaaaa agcaaatcga ggagagttgc
     9541 gagaaaaaca acaacaacgt agagacgaaa aaatagctga aaagcgcaaa gtattgaacg
     9601 aaatagggaa tcctcatggg aaaagaaggg aaaatgttcc gataaaagca aaggtccaat
     9661 ctcaaaagga tagagcacca aagcggattg tgagagcaaa ttcgaatccc gaaatcaaac
     9721 gaaaattcgc tagccaagaa atcatttcaa aaggaataaa ccaatcctca agtaagaaaa
     9781 attcttttca acaagtgaaa cggcggtcta ctctgtccaa acgaaccaat cgaaaagtac
     9841 aaaaaagttt gaaccctcat cccaaaccaa ggtcaggtga gaaaaaatga acgtaaagaa
     9901 attaagtgcc atttcgttaa ctttcctaac aatctctttt atgggtatct tgatgtgtgt
     9961 gggcttattg tttggtgaag acagtgaagg aagtagtagt tcaggtgctc ctcctggggg
    10021 gagtagtgtc tcagaagagg ttttgaaaca ccgtgcaatg gtggaaaaat atgccaaaga
    10081 atcggatata acagagtatg ttccgattct tttagcgatt attcaagtgg aatctggtgg
    10141 cacaatggaa gatgtgatgc aaagttctga gtcagccggt ttaccaccga attcattaag
    10201 taccgaggca tccatcaaac aaggatgtct ctattttgcg tctttggtta agcgttccaa
    10261 agaattaggt tgtgatcaag atagtatcat tcaggcctat aactttggcg gtggttattt
    10321 ggactatgtg gcaaaaaacg ggaagaagca tagttttgct ttagctgaat ctttttcaaa
    10381 ggaaaaatct tgcggtcaaa aaatcgatta ccctaatccc atcgcgaata aggaaaatgg
    10441 tggatggcgc tataactatg gcaatatgtt ttattgctta ttggtcaagc aatacctaac
    10501 tacaacacag tttgacgata aaacagtcca agggattttt gacgaggcgt ttaagtatga
    10561 aggaacggct tatgtatttg gtgggtcaag acccgaaaca ggatttgatt gtagtggctt
    10621 gactcaatgg tgttatgcca aagttggtct gaaacttcct cgggtggctc aagatcagta
    10681 tgatgccatg acccatattg acctaaaaga tgccaaacca ggggatttgg ttttctttca
    10741 ttcgacttat gatgccggta cttatgtgac ccatgtcggt atttatgcag gggagaatcg
    10801 catgtttcat gcaggagatc cagtaggctg gacgaatcta acagaaagct attggcagca
    10861 gcatcttatc ggtgctggac ggtacaaata agagaaaaga ggaaaaagaa tgaagataaa
    10921 aattgaacgg aatcaaaagg aaaagagcct aaaaaagaaa aaagaacgag tcgtatccgt
    10981 gggtaagcat cgaaaaatgg tgcttgccct ttggctttta ttatcttgta gtcttacttt
    11041 tggcgtttat aaaaatttca ccgccattga tcaacataca actcatgaaa aagtggttat
    11101 taaagagaaa gtagtgaata cttctggtat tgaaaacttt acgaaggatt tttcgaaaga
    11161 atatttttcg tggaaaaata acaaagaagc aattgaaaag agaatgaaca acctcgaaca
    11221 gtaccttaca gaagaaggtt tcgctttaag tcaagatatg gttcgagcag atattcccac
    11281 taattccgaa gttcagtcag taaaagtctt agatgttgaa aagagctcgg aggaatttgt
    11341 cgtttcattt ttagtggagc aaaagattac agagggaaaa aaggcacaat cgatttcttc
    11401 tgcataccga gtgacgatct ttgaagatga aaacaggaat tatatagtaa cgagtttgcc
    11461 aactatgatt gcaaaacctg atagggcaaa atacaagaca aaacaagtag aaaatgattc
    11521 gaaaattgat gccaaaacta cagaagaaat cgctgagttt ttggaaacgt tctttaaatt
    11581 atatccatca gcttctgaaa aagaattgga gtattatgtt gaaaatgatg cgatgcgacc
    11641 aatcaatatt gacttaaagt tttcgaagtt agaggatcca gtttacctac aaaacggaaa
    11701 aaaaattcta gtgagtacaa agataaaata ccttgataat gtatcaaaaa c
//
LOCUS       DAKBVG010000077        11686 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000077 DAKBVG010000000
VERSION     DAKBVG010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11686
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.077"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(<1..132)
                     /locus_tag="OUD09_001454"
     CDS             complement(<1..132)
                     /locus_tag="OUD09_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCT9692728.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
     gene            542..841
                     /locus_tag="OUD09_001455"
     CDS             542..841
                     /locus_tag="OUD09_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCT9692729.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            914..2275
                     /locus_tag="OUD09_001456"
     CDS             914..2275
                     /locus_tag="OUD09_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCT9692730.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFLSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLLFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGILTMPIYLYGATYLAPILSDSAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            2297..2557
                     /locus_tag="OUD09_001457"
     CDS             2297..2557
                     /locus_tag="OUD09_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9692731.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2571..3323
                     /locus_tag="OUD09_001458"
     CDS             2571..3323
                     /locus_tag="OUD09_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HCT9692732.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(3417..4022)
                     /locus_tag="OUD09_001459"
     CDS             complement(3417..4022)
                     /locus_tag="OUD09_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCT9692733.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIEQAREWTDHLLTK"
     gene            complement(4078..5052)
                     /locus_tag="OUD09_001460"
     CDS             complement(4078..5052)
                     /locus_tag="OUD09_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HCT9692734.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DCPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(5430..6149)
                     /locus_tag="OUD09_001461"
     CDS             complement(5430..6149)
                     /locus_tag="OUD09_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCT9692735.1"
                     /translation="MPKYEEIANILRERIRSKEYPPDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(6276..7547)
                     /gene="celB"
                     /locus_tag="OUD09_001462"
     CDS             complement(6276..7547)
                     /gene="celB"
                     /locus_tag="OUD09_001462"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HCT9692736.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7700..9097
                     /locus_tag="OUD09_001463"
     CDS             7700..9097
                     /locus_tag="OUD09_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCT9692737.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWNHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSRLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKAIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGTIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            9178..9618
                     /locus_tag="OUD09_001464"
     CDS             9178..9618
                     /locus_tag="OUD09_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCT9692738.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9765..10091
                     /locus_tag="OUD09_001465"
     CDS             9765..10091
                     /locus_tag="OUD09_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCT9692739.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            10092..10841
                     /locus_tag="OUD09_001466"
     CDS             10092..10841
                     /locus_tag="OUD09_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9692740.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10867..11277)
                     /locus_tag="OUD09_001467"
     CDS             complement(10867..11277)
                     /locus_tag="OUD09_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HCT9692741.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNK"
BASE COUNT         3732 a         2043 c         2390 g         3521 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaaatgata ttttacgagg aggaagaaaa
      541 aatgaaaaat cggttaattg tcgcatgtgg aagtggagta gcaacgagtc agacaatcgc
      601 tagtaagatc caaagtatgt tagaagatga cggcatcgca tttcctgtag aagcagttga
      661 ttacaaatcg attcaaaatg agcttctaac tgctggaatc tatgtgtatg tcgcacaacc
      721 tgatgaggaa gtacttgaac aagcaaaaga tttgggtatt gaagtatttc caggaattcc
      781 atttttaact ggaatgggtg tagaacctat ttatgacagt atcaaagagt tagtacagta
      841 aaggaggatt gttggataaa aaagttggtt catagtttta tttactatat ttcgaaagaa
      901 agaaggttta tttatggaag ttttccaaac cattatcaat tatgttttga acttaggatc
      961 agccattttt gttcccctga ttattttgat acttggattg ttggcaggga tgaaattaaa
     1021 aaaagcattc ctgtctgctt tgactttagg gattgctttt agcggaatgt ctatggtcat
     1081 tggctatatg tctaacgcag taagtccagc atcagaagca ttggctaaaa atacgggaat
     1141 cagtttgcct gcacttgatc ttgggtggac aggtgcagca agcatcacat ggtcgtggag
     1201 ttatgctttt atattttttg cagtaacgat tggggtcaat tttgtactgt tactttttaa
     1261 ttggacgaag acgttaaatg ttgatatgtg gaatgtttgg gggaaagctt taactgctta
     1321 tttagtttat tatgtttccg gtagtttggc tgcaggcttt cttactgcaa tggtccaagt
     1381 tatattggaa ttaaagcttg gtgatatgtt tcaaaaacat atacaagatc tcacagggat
     1441 tccattagta acagtgactc attttatgac atctgccgct gtcttattgc tgcttttcaa
     1501 tatgatcatg gataaaattc ctgctttaaa taaacgagcg gatacgaatg cattgaagaa
     1561 gaaaattggg atcttctctg aaaactctgt aatgggattc attattggat tggggcttgg
     1621 ctttgctgct gcgtatggcg tttcaggttc tcttaatcta gcgatccaaa cagctacagc
     1681 attaagtttg tttcctatga tctctaaaat gttcatgcaa tctttgtctc ctctagcaga
     1741 cgctatgtca gaatttatga aaaagcggtt taaggatcgt gaagtatata tcggattgga
     1801 ctggccgata ttagctggtc gttcagaaat ttgggttact gctattttac ttgttcctgt
     1861 gtttattggt tatgccatta ttttgccagg aaaccaagta ttaccattag cagggattat
     1921 caactattcg attgcggtcg gcggattact tttaacagga ggaaacttgg ctcgtatgct
     1981 gatcttggga atcctcacga tgcctattta tttgtacggg gccacttacc ttgcgccaat
     2041 actcagtgat tcagcagaaa aaagcggagc agtcgaggga atcaagaaag gacagttgat
     2101 cacatggtca tccatagaag gaccggagtt cagggtttta tttgcggagg catttaatag
     2161 gaatattctc gctattgtag gatgcgtgat ttttctagga ctgtttgttc tactttataa
     2221 atatatgata aaagcgaaag taccgagtga acgttatgct gaactacaaa aatagcattg
     2281 aaatggtggg aaagtaatga aaagagaaga gacagataaa ataaaatgga cagtcgctct
     2341 ttgtggtaca cttctgctat ttctatatgg actctttact caaaatatca tcatcaatct
     2401 attggtcatt tttttcgcac tagtgatcta taaatatgga aatcacgttt tattcagaga
     2461 gtacgatgaa aaaagaaaac ggaaaataga agaatcaatg aaaataaaag aagctaccaa
     2521 agaaatattg agagaaaaaa gttttataaa aagataatgg aggggtcatt atgtcagatg
     2581 gacgtatgtt atttgagaga gaacgacagg acatggcacg tatcgtaaga gtgattttcg
     2641 aaaggaaaaa cacaaatgtc gcaggaggga atttttcctt taaaaccact gatgatcaag
     2701 ggaaagacta catcatcatg acgccgacca tgatgtcaga agcctatcta ggagagattt
     2761 ctcctgcaca ggttctagta gtagaaccaa gaacgcgtag aatcgttgac ggagtcggtc
     2821 atttaactcg ggagatcaat atgcatgaag cgatgtatga gactaatcca gctatcaaag
     2881 ctgtcctaca tgctcatgcg cctaatgcaa tgttctgggc gactactggg ttgcctatgc
     2941 ctaatttgac agaagccaca gctaaagtaa aagagattcc tgtcatggac tttgagccta
     3001 attgctctga agaacttgct gaattgacaa gcgactatgt aaaaaaacag catctcgaat
     3061 taccgcatat gttgctgctc aactctcatg gcgtattgat caacacaggt ggagaatcag
     3121 gaatcgaagc tatccataaa gcactgcaaa tccttgatac agttgaatgg aatgcagaaa
     3181 ttgcctacaa gcagacgatt tttcaagcat tagggattac tgacggtttt tattcaaaag
     3241 gtgagaaaat cgggggattg gaagagttga aacataaaac ggctatttat aataaatctg
     3301 ttattgcagc tggtggagac taacctatct gacacaaaaa actacgactt tcacaaaatc
     3361 tgatccagat cgatatctgc ttcaggttgt attatgtaag tcgtagttct tttcttttat
     3421 ttagttaaga ggtggtctgt ccattccctc gcttgctcga tttcgtccat cgtcaactga
     3481 tgacctgtta cagttagctg aatagtcaca tttgcttggc gtgtttcgaa ttgatcggcc
     3541 aattctttaa aggaagcagg ggaaacgatt ggatcgttat caccataaga aatccagacg
     3601 gttttatcct ctaatgtgga acggctttga tgaacaccta gagacatcgg atggaaaaac
     3661 aaccctttat ttagctcggt ctcacgctcc aataataaat gagcagcgat gtttgcgcca
     3721 tttgaatagc cgacaagcag ccaatcactt aacggtatca gttctttttc gctgatttct
     3781 ttgatttttt catggagttt atctgtttct ttttccaaac tgtccaggtc gaattgattc
     3841 aatccattac gcttgaaaaa acgattcatc ccatcttctt ggaccgtgcc acgaaaagaa
     3901 agcaatgtac tgtttggtgc aagaaagtga gcgatatcca gtaaagaatg ttcatcgcca
     3961 cctgtgccat gaagtaagat caaatgcttg ccacttgttc ctttttcaaa aatgtaggtc
     4021 attttgttcc ctcttttctg tttctataga tgcaaaaaat gaagtagcat gaagagatta
     4081 ttcacgtttt ttatttgcat cacttgtatc gaaaggacgg acataggctt caatggcttc
     4141 acgacgttct tctaagaatg gaggaagaga tagtttttct cctgctgatt ctgctggttc
     4201 atcttcaagg aatccaggac aatcagttgc taattcaaac agtacgtgat gagcggctag
     4261 gaagtactca gattggaaat agaaacgatc gacgaaacca gaatttggca attcaatctt
     4321 attgattact tgaatccagt attccaatgc ttctttgtct gctacacgca atgctaagtg
     4381 atggatattt ccaaatcctt cgattgcttt tggcaagtct gtccgatgtt caataatgat
     4441 gcttgcacca tttccgcctt caccgatttc aaataagtag aaatctcctg aatggtctgt
     4501 ttctttgaat cctaagattt tttcaagaat caagcggata tgctctaatt gactgacacg
     4561 gacaaagaca ggtccaagtc ctgtgatcgc aaactctgtc ggtacattgc tgtttttcca
     4621 aggaatgcca gctgctacac cttgattgtt ttcgtcagag atcaattgat aatactgatt
     4681 atcgaaatct tcaaattcga tatatttttt gccgaaacgt tcattgattg ctgaatgagt
     4741 aacgtcatat ttttcaaaac gttccaacca aaagtctaat gctgcgtcac ttggcacacg
     4801 gaaagaagca cgagagatcg tatctgttcc tttttttcct tttggctgat ttggaaaatc
     4861 aaagaaagtc atatctgttc ctggcgcacc agtatcgtct gtaaagtaaa gatggtaagt
     4921 ttcgatgtca tcttggttaa ctgttttttt gattaagcgc attcctaaaa tatctgtaaa
     4981 aaagcggtag atttttgtag cgctagatgt cattgctgtg acgtggtgaa ttcctctgat
     5041 ttgtgtatcc atgataaatt tcctcctgtc aacttacaaa aagtaagtag ttgatttaga
     5101 catagtatag agggtgcatc tctattttgc aattaatttg tcttctaaag tatcaggaag
     5161 gaatcaagaa tggaaaagag gagggacgga tcaataaaat aaaaagactt acgactagct
     5221 gtatcgattt caaaaaaact agctttaaaa agctaacttt agaatcatta cgggtggtca
     5281 taagtctttt tatgctgagt tattttttca tttttgagct aatgcttagg ataggataga
     5341 atgttgattt cacgatctct tcttgttact tgaaactgct tactactgtc acgatctctt
     5401 ggtaaacggc tttttcacaa cctcttcatt taagttgaac gcacatccag cacagaatag
     5461 ttccgaacat caaatcggtt tcggctggaa gaatattcaa tcggctgtcc atctttgaga
     5521 tagacgactt gctgaacttc cagtacagga tcgttttctg cacagttcaa atattgctga
     5581 tcatattcat cacttttatc tgcagaaata gcacgataag cccctgcaaa atggacattc
     5641 aattcttgat gaagatgatc atagatagag gctaacaaat gttctttttt taaacccggc
     5701 acaagatgga ctggcataaa agtatgctct aaaacatatg gctcgccatc taagatcctt
     5761 aaccggtgaa tattgtagac aggttgctcg gcatcgatca tcaatcgttc ttggatatat
     5821 tgattaggga attcaacatc aaaatgaatg atttgactct tcaaagaacg atgtctgcgt
     5881 ttcatttgga gactcaaacc ttcatactcg ttagcgggcg atgtgtcttt ttgcataaat
     5941 ggatgattca agattttcgt tcctgatcct cgctgcgaat agacaagtcc ttccattgct
     6001 aagatattga tggctttttt gattgtcatc cggctcgcac caaattcttt gaccaagtcc
     6061 acttgattag gcaaaaaact atcgggagga tactcttttg aacggatacg ttctcttaaa
     6121 atatttgcga tttcttcgta ttttggcacg ttattcacct cttctagagt ataacgaaag
     6181 attatgcttc cgacaactcg aagtagcatc tggaacattg tttattcgaa aaaagaggtc
     6241 gtgacaagtt tttgtcacaa ccccttaatg atctattaag caatatctaa atctggttgt
     6301 ttatctagta ctttcaggaa tgggtaccag ataaaggcaa cgatgacaaa gtcgatgact
     6361 tgcagcatac caccaagcca tgaatttgtt gctaagacac cgcttgcaat gattggcaca
     6421 gtccatggaa cagagacgcc agttggtgca gggacaagtc cagcagccat taccaaataa
     6481 ttgaacgtcg ttacgattag aggtgccaca acccaaggaa taaggattgt tgcattcaat
     6541 acgatcggta gaccgaagat cgctggttca tttacattga aaataccagg tgctaaagct
     6601 aatcgtccaa catcacgata ctgtttcttt ttaagaacaa aggccatcaa taaaacgaca
     6661 gctaaagtca ttccagagcc accaatacca acagtgaagg tttccatgaa tggttttgtg
     6721 atgatgtgtg gtaattcttt tccatgttga taagcttcta agttatcaag catcagtgta
     6781 ttccaaatcg ggtccattac agagttgacg atgatttgtc cgtgaagtcc gaagaaccat
     6841 aagaattgga caaagaacaa agcaatcaat gttgcaggta aactacttcc caaaccagtt
     6901 aatggttttt ggatcacttc ataaacgacg tcatgtaagt tagcattgaa aataccagtc
     6961 atcacagcat tgattactaa gaaaatagtt agtgtcacta cagcagggat caaagcggca
     7021 aatgattttg ttactgcagg cggaactcca tcaggcattt tgatctgcca gccgcgtttc
     7081 gtgattctgc agtagatttc tccagccaag aaagcagcga tcattccaat gaacattcct
     7141 tttgcgccaa gacgatcaag tgaaagcaca ccagtcacat cttcaccgcc agcggttttc
     7201 attgcaaaag gcgtaagaat caagaagctg gcaaaagaca cggcaccacc gaaaatacct
     7261 tcaacgtcat aagattttga taaataatag ccgataccaa aagtaacaaa aaccgacatg
     7321 atgctcattg ttgcattttg cccattaccg aataagttcg ataaagttcc ctttgttgcg
     7381 tcactgaaaa aaggcaagtt attgataacg acaacgatcg aaccgaacat cgttaaaggg
     7441 aaggaaagca tgaaagcatc ccgcagcact gtaaggtaac gattttgccc taataaatta
     7501 gctaagggca tgattttctc agaaagcttg tccataaaac cgttcatttt tattcacctc
     7561 aaatttattt gcaatcgatt acatacaaag tataacgcca aaaattttaa atgtctagtc
     7621 attttcataa aaagtattga tttatttttt agggttcgtt atgattacga tatcaagaaa
     7681 aaagagagga agtaaaaaaa tgaaaaccta cacattccca gaaaattttt ggtggggttc
     7741 tgctgctagc ggaccgcaaa cagaaggaag agtaagtgat gatggtaaag gagaaaatat
     7801 ttggaatcat tggtatgaat tagagcctga aaaattcttc gatcaagtag gacctgaaaa
     7861 aacttcgcaa gtttacacaa aatacaaaga agatgttcag ttaatgaaag aaactggcca
     7921 taacacattt cgtacatcta tccaatggag tcgtttgatt ccagaaggga caggtgaaat
     7981 caatcaaaaa gctgtttctt tttatcgaga ttattttact gaattgctgg ataatggtat
     8041 cgaacctttt gtgaaccttt accatttcga tatgccacta ccactgcaag aaaaaggcgg
     8101 gtggttatca agagaaacgg tcgacgctta tgtttcttat gcagcagcgt gctttgaact
     8161 gtttggagat ctggtcaaaa aatggtttac tcataatgaa ccaatcgtac ctgtagaagg
     8221 aggctacttg tatcaattcc actatccaaa tcatatcaat atgaaagaag cggtccaagt
     8281 aggctatcat gagaatctag caagcgcaaa ggcaatcaaa gcctaccatg aaggcggaca
     8341 tgatggtgag atcggaatca tcttgaatct gacaccaagc tatccgagag atgaaacgaa
     8401 cccagaagat gtaaaagcgg cgaagatcgc tgacgcattc ttcaaccgat cattccttga
     8461 ccctgcagtg aaaggagagt ttccacaaga gttgatcgaa attgtcaaag aattggctat
     8521 aatgccagag atccatgaag gagacctaga actcatccgt gaaaacacga ttgacctgct
     8581 aggaatcaat tactatcagc ctcgtcgaat caaagcaaaa gaaactccaa tcaatcacga
     8641 acatagcccc atgccagatg attattttga taattacgac atgcccaata aaaaaatgaa
     8701 tccatatcgt ggatgggaaa tttacgaaaa aggaatctat gatattttaa cgaatacaag
     8761 agaaaactat ggtaatatca aatgctatat ttctgaaaat gggatggggg tcgaaggaga
     8821 agaacgattt gttaacagcg aaggaactat tgaagatgat taccggattg agtttgttac
     8881 ggatcatttg aaatacgtcc atcaagcaat ccaagaagga accaattgtg tgggttacca
     8941 catgtggaca tgtatggaca actggtcttg gacaaatgct tataagaatc gctatggctt
     9001 tatcgctgtt gatttggcac aagacggtaa acggacgatc aaaaaatcag gtcgctggtt
     9061 taaaacggtc tctgaaaaaa acggatttga tgcctgatat tttttgataa aacgttcaca
     9121 aaatgtttgt tctaacactg taatcgtgct acactaacag tgaaggaggc gtttacaatg
     9181 gtagatactt atcgtaatat tttagtagca attgatggtt ctgaaaaggc agaaaaagca
     9241 tttcttgagg caatcacgat tgccaaacga aatcaggcaa cgttgcacat cctgtatgtc
     9301 aatgaagtaa caggcaatta ctttggtgat tttgcatttg ttacaacaag ccttcaggaa
     9361 gaattggatg aggttgcgga aaaccagatg aaagaacatc gaaacttggc aattgaaaaa
     9421 ggcttgacag atatcgagac atatgttctt tacggttatc caaagacact gattgctaat
     9481 ttccatgaat cgaaagaacc aatcgatctg attgtcatgg gggcgacagg tctgaatgcg
     9541 gtagaacgtg cactagtagg ttccactact tcctatgtag tcaaccacgc atcatgtaac
     9601 gtcatggttg taaaataaat aaattaaggg aacagaaagc aaaaaagcat aacgcttctt
     9661 ttgctttctg ttcctttaat tttttgtaaa aaattacttt acctgatgaa gtaatcatgc
     9721 tattctaatt acaacgacag atggagtaat atggaggaaa gaaaatgatt tcaagtgatg
     9781 gaatacgcgg atacaatgat gcgattattt tatcgatcct taacaaaaac gattcttatg
     9841 gatatgaaat ttctaaaacg attcgtgaga ggacaaatga ttcctatgtg atcaaagaga
     9901 caacacttta ctccgttttt gcccgtttag aaaagaatca actgattgtt tcgtatcctg
     9961 gagaagtgac tcatggaaaa aagcggacat attataaaat cactgaaata gggcgtaatc
    10021 ttttagaaga aaaaaagcaa gagtggctga cgacgaaaga cgtagtcgat caatttttgg
    10081 agggaaatta aatggataca atccgtaatt atttagacag tctttttatc ggagtacctc
    10141 aaagtaccga gatagataag ctgaaaactg atttgttggc aaatatggaa gaccattatc
    10201 atgaattgat gggagaagga aaaaatgaac aggaggccat cggtacagta atttcgacat
    10261 ttggttcaat cgatgaatta ttagaagaac tagatgtcga aaaaaaacat caagctgacg
    10321 agacggaaac aaacacagct tctatttatt taagcgaagc agaaaactac tggaaagaat
    10381 acagagcggc aagtctccaa gtggcatcag gtgttctatt cattagctta tcttttgcaa
    10441 gttttctttt cttttgttca gcagggtatg tgttcatggg tatttcttgt ctgattttcg
    10501 gtatagcatt agctgtcggt ttttttattg cttctggtat gaaaatcaca cgtttaaatc
    10561 actttttgca ccatcggaaa atacctgaaa aagtattagc agaagcaaaa gaaaaagaag
    10621 aagagtatca gcgaagtttt ggcttctcac tgattgcagg aattggatta tgtatttttt
    10681 cacttttccc acttttggct tctttaatgt ggtatatgga tggtagtatc ggtgcctcta
    10741 tattttttgt tacggtagga acgggagtct tcttgatcat ttatggcagt ctggtccgtc
    10801 attcttatcg tcaattcact caaagtgctt attactggtg aatgtattca gatttgtaaa
    10861 aatattttat ttattgctta cccatgtaaa atcgttatag acagatttca gttgacttct
    10921 gcgccattgt acagggataa aaagacgcat actatcttca ttgaatccga ctaataattt
    10981 ctttccgtca gtaatgatgg gacgttgtaa caaagtaaaa tgcgagacga tcagatcgac
    11041 aagctctgag gtcgtacatt ggtcgatttg atctttcaaa tttttataat caatcgagcg
    11101 tttagctaat agatcgtcaa atccgttttc tgttaatgac aataaataaa atatctcttt
    11161 ccttgttaaa ggctgtttag gtgtgaaaat tcgttctctg taaggcattt gatggtcttt
    11221 cagccagttt gccatcagat ggtaactatg tgtgcgagaa gaatagatcg taagcataaa
    11281 aaaactcctt aaatagtcca ttaatttgtt agatctgttg agataaataa taggtaagga
    11341 gctgtgcata agcattgatc aaaacaaccc aaacatcatt agaaataact ttctaaagtc
    11401 ttaaacttgc gacgagaaac aactagacta atagaaaaaa tgtcttagcc atttggcttc
    11461 ctgtttgttt ttttgcttat gctgcagctc ctcgggaaag ggttctaaca atttgaagaa
    11521 ggttgatgat tgatctagtt taaattgtgt gaaatttgaa atctggtgtt tttactagat
    11581 gaatcaccta acaactatga ttataactta tagaacttct gttttgtact tggttttgtt
    11641 atagttgtta taaagtttta ttttattaca gaacattttt aattaa
//
LOCUS       DAKBVG010000078        11579 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000078 DAKBVG010000000
VERSION     DAKBVG010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11579
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.078"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(145..2220)
                     /locus_tag="OUD09_001468"
     CDS             complement(145..2220)
                     /locus_tag="OUD09_001468"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCT9692742.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEIIPFEILDVKTDRVSRGHRFLGEDVTFTHAKEYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQITAMALSL
                     ASLDQIITK"
     gene            complement(2222..3139)
                     /gene="glyQ"
                     /locus_tag="OUD09_001469"
     CDS             complement(2222..3139)
                     /gene="glyQ"
                     /locus_tag="OUD09_001469"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCT9692743.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            complement(3523..4335)
                     /gene="recO"
                     /locus_tag="OUD09_001470"
     CDS             complement(3523..4335)
                     /gene="recO"
                     /locus_tag="OUD09_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCT9692744.1"
                     /translation="MSQLVESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            complement(4478..5377)
                     /gene="era"
                     /locus_tag="OUD09_001471"
     CDS             complement(4478..5377)
                     /gene="era"
                     /locus_tag="OUD09_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCT9692745.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5392..5787)
                     /locus_tag="OUD09_001472"
     CDS             complement(5392..5787)
                     /locus_tag="OUD09_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HCT9692746.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(5771..6247)
                     /gene="ybeY"
                     /locus_tag="OUD09_001473"
     CDS             complement(5771..6247)
                     /gene="ybeY"
                     /locus_tag="OUD09_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCT9692747.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6264..8456)
                     /locus_tag="OUD09_001474"
     CDS             complement(6264..8456)
                     /locus_tag="OUD09_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HCT9692748.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVVFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEVQEE"
     gene            complement(8480..9451)
                     /locus_tag="OUD09_001475"
     CDS             complement(8480..9451)
                     /locus_tag="OUD09_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCT9692749.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10156..11535
                     /gene="brnQ"
                     /locus_tag="OUD09_001476"
     CDS             10156..11535
                     /gene="brnQ"
                     /locus_tag="OUD09_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HCT9692750.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
BASE COUNT         3137 a         2430 c         2127 g         3885 t
ORIGIN      
        1 ttttcatgag cgttttgaaa attctagcaa agaataaaat aacaaaaatt attaaaatag
       61 aattaataac agcttttata tttataacaa aaaaacagag acacagcacg tttcgtgcat
      121 gctctctgtt tttcaaacaa aagactattt tgtgatgatt tgatccaagc ttgctaatga
      181 caaagccatg gcagttattt gactaagttg tgttagtcgg ttcttacgta ctgctggttc
      241 atcagccatg accatcgttt gatcaaaata attttcgatc agtggacgaa gagcgacaag
      301 ctggtcgtag ttttcgtgga tcgtttggtc agcaaaactt gctttcattg cttctactgc
      361 tttgtgtaat tctttctctg cgtcgttttc gaacagctca gggtctactt ctgattgttt
      421 ttcaagctgc gcttttttcg ctaagttcac gacacgtgtc aatgcttcca ttgaaggttt
      481 gaagtcttca tctgcaagat gttgtttgaa caattgtgct gctgcaaata cttttgtcag
      541 atctttttgt tctgcattca aaacagcttc gatgacatca tgacggatat tcttcgttgt
      601 caataattgg cgcagacggc ctttgataaa gtcgatcacc tgttgttgtc ccgctgacaa
      661 gccaatgcca aaacgatccg tatctttact gataacttca ctaatcgttt cttgcagaac
      721 agacaaagga aaggcccatt ttttcgattc gatgattcga acgacaccat atgcttgacg
      781 gcgcaaagca tatggatcgt ttgagccact tgggatcatc ccaactgcaa agaaagaaaa
      841 gatactatct aatttatcag caatcgctaa aacagaacct actgtagtct caggtaaatc
      901 tccttcactg gatataggca tgtaatgttc acggatcgct gttgctacgg ctggtttctc
      961 gcctttaagc agagcatatt tttctcccat gatcccttgt agttcaggga attctcctac
     1021 catgttggtt actagatcaa atttatagat ttcagcagcg cgttgcagat ccatcaattc
     1081 ttcctcagat agtcctactt gttcgcctat gatgcgagcg atgacttgta cccgttgcat
     1141 tttctcatag atgctaccga ttttttcatg gaaagtaaca gtttttaatt tttcaacgta
     1201 ttggtcgatt gaagctttct tgtcttcatt ataaaagaat tccgcatctt ctaaacgagc
     1261 agtcaatact ttttgattgc ctcgtaccac attttccaat tttacccgat cgccattacg
     1321 agcagaaata aaatgaggaa gcaacatacc ttgatcgttc cgtacttcga aataacgttg
     1381 atgttctttc atagaagtga ctagtacttc ttcaggaaca gatagatatt tttcttcgaa
     1441 gtcgccagaa aagacagttg gatattcgac tagattgttg acttcttcta gtaaatcttc
     1501 atctagatca atgatccagt gatttttttc tgctaattcg tttgcttgtg tgatgatcat
     1561 ttcttttctt ttttgcggtg aagcaataac aaattgttca ttcaattttt cttcgtattc
     1621 ttttgcatgc gtaaatgtca catcttcgcc taagaaacga tgacctcttg aaactctgtc
     1681 tgttttgaca tccaagatct caaacgggat tatttcgtca tctaacaatg ccacgatcca
     1741 gtgaataggg cgaatatatt caaaatcgta ttctgcccag tgcatcgtta ctgggaaagt
     1801 gaggctttta atgacatctg ctaaacctgt caatacttct tttgcttctt tgccgtgagt
     1861 gtatttcgtt acatagacat attccacgcc ttttaattct ctaaaagtga tagcatctgt
     1921 cgtcagacct tgtccgcgga caaatccttc agctgctttc gtccagtttc catctgcgtc
     1981 caaagcaatt tttttggctg gtcctttgac ttcttcttcg ctgtctgctt gacgttctgg
     2041 aatgtttgtt actttgattg ctaagcggcg cggtgtagaa aacgtttcga tcgtatcata
     2101 agtcaattgg ttttcatcta aaaattttgc tgttttttct tctaattgtt tcatgcttgg
     2161 tgtgactaca tgagcaggca tttcttccag cccgatttca agtaataagt cttttgccat
     2221 cttattcagc ctccttcacg ttttctggat ttgctgtgtt tttattcaat aaagggaatc
     2281 ctaatttttc gcgttctgcc acaaagattt tggctacagc acgagccatg ttgcggatac
     2341 gggaaagata acctgcacgc tctgttacgg atactgcacc gcgagcatct aataaattga
     2401 atgtatggct acatttcaaa atataatcat acgctggatg aacaagattt tcatcgatac
     2461 agcgtttagc ttctttttca aaacgatcaa aattgcttag tagcaggtct tggtcgctta
     2521 cttcaaaaga atatttagaa tgttcatatt ctggttgttt gaagatctca ccatatttca
     2581 cgccatcagc ccattccaga tcataaacac tttcaacttc ttggatataa gaagcaagac
     2641 gttctagtcc ataagtgatc tcagaagtaa ctggtttaca aggtagtcct ccgacttgct
     2701 ggaagtacgt gaattgtgtg atctccatcc catcaagcca tacttcccag cccacaccgg
     2761 cacaacccat cgatgggttc tcccagttgt cttcaacaaa gcgaatatcg tgttctaatg
     2821 gatcaattcc taaaagacgc aagctttcta aataaagctc ttggatattt tcaggtgaag
     2881 gtttcattac gacttgaaat tgatgatgct gatacaaacg gtttgggttt tctccataac
     2941 gcccgtcagc tggtcggcgg gatggctcta cataagcagc attccatggt tccggtccga
     3001 ttgcccgtaa gaatgtatat gggctcatcg tacctgctcc tttttctgta tcataagctt
     3061 gcatcagcat gcagccgttt gatgaccaaa atttttgtaa tgttaaaatc atatcttgga
     3121 cggtcaattt tttactcatc tgttttgccc ctttcaattt ttaagcgttg ctgtcaataa
     3181 gaatacagga aagaaaacga caaaaagtcc ctatgtctga ttacagacat agggacgaat
     3241 aatttcgcgg ttccacccta cttccgaata aagattcggc agcttcattt atcgtactcg
     3301 caagtgcctc ttcaaaaatc gtttgggtcc ggcttccact ctcccgaact cgctaaacca
     3361 cgtgtttttt actgatcttg gtcaatgtac gtattcattt tgcagttttc tggtttgtat
     3421 aatagatcct ctcagtgaac gatctattta atctacggtt aataatagac ggaagtccct
     3481 gtatttgtca aatagttttg atttttttgt tgtatctttc atttattctt ctttttcagg
     3541 tatctctttt tcaggcatat ttttctctgg cgtctttaat acgtgtgccc aagacttcat
     3601 ttgatcaatg aactttttgc tttttagatg aataccgaca tattcatcat acaactggtc
     3661 aatgaggtca cggattgcct gcttcgtttc ttctttcaag tggattccac tgatcttatc
     3721 ataagaaata gccgaaaaca ttcgaacgaa ataaacagcg cgaggatctg catgataacg
     3781 gtgttcgtcc attggccagt gtttctcgca tatcacaccg ccatatttgg aagaatagtc
     3841 aaattttcct tttgtttccc cgcatacgac acagtgcgtc caaattggcg cgattccgaa
     3901 gcgttgcagt atttgcactt caaagatatt cgtgatgatt tctgcatcat ctccttggtc
     3961 catccgctgc agagcttgtt gcgtaaattg gaataaatgc gggtcatatt gatgatcttc
     4021 gattgctgca tctactagat ttagcaaata cgtaccataa ccgctcaaaa agatatcttc
     4081 ttggatgttc cgaaacgcat ggacgtcttt gacactgctt aaaaacgaca aaccttcact
     4141 tcgaaaatta ccgatataga ctgcttcggt aaaggggagc aaagccggag ctaatggctg
     4201 attttgccga tggattcctt tgatataaaa catcattttg ccagaagatt ccgtaaagat
     4261 tttgactaat ttatcttttt ctttgtaatt acggctagat aaaatcaagc ctttcgattc
     4321 tacaagctgg ctcatattct ccctcctaaa aaataaatct ggaaaagcag attctgccga
     4381 tccagattat ttatgtttcg atcttccgct gtttttctca acgtacggat ttttatccag
     4441 tttatttcta tacctaacct ataagaagtc cagaatatca gtactcgtct tttcggtagc
     4501 cgaagtcttg cagatagact tttttgtctc gccagtcttt ttggactttg acccaaagtt
     4561 ctagaaatac tttgtctcct aaaagatttt cgatatcctt tcttgctttc gttcctactt
     4621 gcttcagcat ttttccgcct ttgccgataa tgattccttt ttggctatct ctttcgacga
     4681 tgatcgtggc ctggatatgg attttatcat tctcgtttcg cttcatcgaa tcaacaacga
     4741 ctgcgacaga atgaggcact tcgtctcgtg ttaaaaacaa gactttctca cggatcaatt
     4801 cagaaacgat gaagtattca ggatgatcag tcacttgatc atctggaaaa tattgtgggc
     4861 cctctggcat ttcactgact aatacatcca ttaagcgttc gacattgttt ccttctgtag
     4921 cagagatcgg aatcacttgt gcaaaaggca tctgcgtcga ataatcttcg atgattccca
     4981 gcaactcgtc tggatggatc ttatcgattt tattgataat cagataaaca ggagaatgca
     5041 cgttttttag acgttcaata ataaaatcgt ctccccggcc acgtttttga tcagcactga
     5101 tcataaataa gactgcatcc acttctttca acgcactgta agctgcttct accataaaat
     5161 ctcctagacg atgctttggt ttatggattc ctggtgtatc gataaaaatc agctgagctt
     5221 ctggtgtagt atatactcct tggattttat ttctagttgt ctgtgccttg tcactcataa
     5281 tggcaatttt ttgacccacg attcggttca ataatgtgga ttttcctaca ttcgggcggc
     5341 cgacgattgc gacaaaccct gatttatgtt ctggcatata cgttctcctt tttaattaaa
     5401 caacaaatga tatattttag ggacaaataa aatcaaccca atgatgacag caaaacaagc
     5461 ggtcactaaa actgctccag ctgccatatc tttgattttt ttcccaatcg gatgaaaatg
     5521 aaaatccgta aacatgtcca ctacattttc aaaaacagta ttgatgatct ctactatcca
     5581 aacgagaaaa acagctaaaa gcagccataa ccactctatc cgatcaagct ggaaaatcac
     5641 gcccataaat aaagcaagga taccgaacgc aacatgtttc ctcatattgc gttcttcctc
     5701 aaaaacggtt ttgactcctt ggacagcaaa ttccacagac atcaggaaat gtttgttttt
     5761 ctctacgttt ttatcttttg agtccatagg catccaagat ctctttctgt aaaccaaaca
     5821 tttctttctc atcttctggt gtcatatggt catacccgtt gatatgcaaa aatccatgta
     5881 ccgctaaaaa gccaagttcc cgatcatatg aatggccgta agcttctgct tgttcagcag
     5941 cacgttcaat agagatcatg atgtctccta gtacacgagg taatggattc tctccatctt
     6001 caaaaatcac tgggatttcg tcttcgcctt cttcttccag agcaaaactg atgacgtctg
     6061 tcggtgcatc ttttccacgg taatcacgat tgattacttg aattgccgca ttgtccatga
     6121 aagtaacaga catttctgtt tcctctggca gctttaaata agatgctgca aattgcagca
     6181 agtcatcgat ttctttcatt ttctcttctg agacttggtt tgtttcatcc ataaaggtaa
     6241 tatccattat acatcgcttc tttctattct tcttgtactt cctttatctt ctcaacttct
     6301 gttttcattt taggatattt gatccgagaa tggaaggtgc tacttaaacc gctgattaat
     6361 gatttttcca cttttcggat ttctttcaag gtcagtccac tatcgtctaa ttgaccatcg
     6421 gaaatacgct cttcgataag attatggaca aaactttcga ttttttcgct cgtcggatga
     6481 tccattgccc gtactgcagc ctcacaactg tcagcaatat tgacgatccc tgcttcacga
     6541 ctttgcggtt ttggtccagg ataacggaat tgctcttctg ttacttccgg attgcgttct
     6601 ttcgctttga tgtagaaata acgcattagc gtggttccat gatgctgttt gcaaatatcg
     6661 atcaccattt gcggcatatt ataatcttcc aatattttgg ctccatctgt cacatgtcca
     6721 aagataatat gtttactgtc ttctggcaat aagaaattgt gcgggttttc cgctcctgaa
     6781 ggcaagtttt caacgaaaaa gctagcatgt ttgatcttgc cgatatcatg ataatagcaa
     6841 gctactcgag ttagcaaaga acgtccgccg atttctgcta cagcgttagc acttagatta
     6901 gctaccatca tactatgatg ataagtacct ggcgcttctt ccagcaactg tttcaataaa
     6961 ggatgatttg gattgctgag ttcatttaat acaattacac tgtcatcatt gaccatcaat
     7021 tcaatatatg ggtgtagacc gatcgtcagc aagaatgaga agagtgcacc agtgaacccg
     7081 cagacgagca ttaaccaagt cgttgcatca ccaaagctca ttccttgata gacgatcaaa
     7141 atgacatcca tcatcacagg aaaaataatg acccacatgg cagcagataa tccttgttct
     7201 gagatccgtt tgcgtctgac aatcgttgcc atgagcccag agaaaacata agacatcaag
     7261 ataatcgtta gaaagttcgt gccaatcgaa tcataaaaaa tgaaaagtgc ggatactgct
     7321 tggaaaatac cagccatgat ccctgctctt cgattcaaga aaaagcttaa aacaagtggt
     7381 gcaaaagcag cgggataaaa cagcgggata taagtagctt gttccgtttg gaataattgg
     7441 aaaaatttca tcagcactat gctgactgac atagctgtca catagaaaag cacgtatttt
     7501 gttctttctc ctgcattttg gaattgaagt gaattataaa tcagtacgcc gatttgaagc
     7561 aaaacagcta aaaccatggc aattagcgga aaaatcgaag ttgtttgatt agtcagtcct
     7621 agtaattcca attttttcat agctgaagca tcgatttgat taccttcacg aacaatgatt
     7681 tctccttgga agatcatcac tggttgtaca gcttctcgtg cggattgttt caattcttct
     7741 gtctttttct cattcaatac gtcattcact acgatacctt gatccactaa gtagcgaatc
     7801 atttgttgtt gtgctggtgt gacattcaaa tattggattt gctcttctgc ttcttgttta
     7861 aaagcttcca attcactttc acgaacttgt ttgctcatct gttcgtcaat gagcttcaga
     7921 ctctcatccc tcacagtatg aatctcagtt tcagtcattt caaatatcgt cgaataaaaa
     7981 ttgttcggta gtttttgata aaacacaaca ttttctgcat tcacattttc aaaatttttc
     8041 ttcagcgcgg cgatccgctc ttctgtagta ggctttggga tattttcatc ttcctttgcc
     8101 ttatcaatct tttcgtcata agatttgttg atggcatcat tcgtcttatc gatcaattca
     8161 aatagttgct tgatccgatt gttctggtca tctgcaagat ctttttggta agtgtattct
     8221 ggagtgactg cttctgctgc cagttttctt ttttgttcgg tttcttccgt gttctcgatc
     8281 gttttgttcg cacggatact ttcttctgct acttggcctt ctctgaaatc attggccttt
     8341 tgtttgacac tactaaatgt aacgccgcag ataatgataa aaaacaagaa cagcaggaaa
     8401 ggcaatgctg cctttccaag tttttcttgt agtttctgca gtgttttatt caacatgtgc
     8461 ttatcctcct aagcaaagct tattcttgat ggagatctgc cttttcataa gctttgatga
     8521 tttcggcaac tacagggtgc cgaacaacgt cacttgcttc aaaattaaca aaatcgattt
     8581 tcttgatttg ctttaatgtt cgttctgcat gaacaagacc acttgtcgtc cctcgaggca
     8641 agtctatctg acttgtatcc ccattgacaa tcatttttga ttgataaccg agacgggtca
     8701 agaacatttt catctgagcg actgtcgtgt tttgtgcttc gtccaagata acgaatgcat
     8761 catccaatgt tcgcccccgc atgtaagcta aaggtgcgat ttcgattacg cctcgttcca
     8821 tcaatcggtt cgtatgatcc aatccaaaaa tttggtataa agcatcataa acaggccgca
     8881 gataaggatc cactttttct tttagatccc cgggaagaaa gcctagattt tcacctgctt
     8941 ccacagcagg ccgcgtcaaa atgatttttt gcacttcacc ttttttcaag gcagcgattg
     9001 ctaatactac tgcaagaaaa gtttttcctg tcccggctgg tcctacgcca aatacgatat
     9061 catgtttggc taccgattcg acatatttct tttggccgct gttttttacc cgtataggtt
     9121 ttccattacg gtctttgacg atctcttcct catacatctc aacaaaataa tctaatgtac
     9181 ctttttgccc cattttcaat gccgtcacaa catcgggcgt acttacgtgg attcctcgtt
     9241 tgatcaattc ctgtaatgtc cgaatgactg aggcagccaa ggaaatctgg gattgctcac
     9301 caatgatctg aatgatttct ccacgtgtat ggatggttgt ttccgttgtt tcctcaatca
     9361 gttttatatg cttgtcgtgt gcacctaaaa gcatgctgac atcatcagca gccgtcaatc
     9421 taatctctaa agaactagac tcttccgtca aaaatagaca tccccttttt ttcgattcat
     9481 tctttcctat tttaccatac tcagaagaaa ctatatagca aataagaaaa ggttctaaaa
     9541 gagttgctta accataagta ccaagaaacg ttaaaaaata gctgcttcca ttctctaaag
     9601 gtctcgtttg aataaaaaaa gctgatcatg taagctatgc ataagataga ggacaagtat
     9661 tccgtctaac ttagcaagta ttctctgggt atttttcttg tttcaaaaaa agaaaacaaa
     9721 atatttgaaa tattcttgca tttcaaaaaa aacaggtgta ttataacgtc aacaaataaa
     9781 tatacagcaa agcgtcgagg aaaacagtag gctattcagt gatttattcc cagtgagtca
     9841 tgtaagtgag aaatgacaat aaatcgaata gacgaacatc atttccgagt caatgtcgaa
     9901 aacgtagatc attgtggctg cctccattat caggccagcg gataacttta tcgaaaagcg
     9961 tccgtgaatg aagaagcttt tttaagcgaa aaaaggtggt accgttctag aatctagagc
    10021 ccttttccca agtggaaaag ggcttttttt atattttaag gtggtggttt tcatggaatc
    10081 tgatattagt ggtatttcta gtttctgcaa cgtttatttt gttactcaaa atgataatta
    10141 ttggaggaaa aatttatgga agaaacaaaa acttattcat ggaagcaatt attgatggtc
    10201 agttcactta tcttcgggat gttcttcggg tcaggtaatc tgatctttcc tgtccactta
    10261 ggtcaaatgg ctggatcaaa ctggatcagc gctggtgtag gatttgccat ctcaggcgct
    10321 cttttcccat tattagctat cttagctgta gtagtgacaa aaagtgatgg tctgtatgac
    10381 ttagctaaac ctgtcggaaa atggtatgct gccttgttct tgatccttgt tcatttaacg
    10441 atcggaccat tctttggtac accgcggaca gcagcaactg cttttgaaat ggctgctaaa
    10501 ccctttttgc ctgaaaaata tgattcatta ggtatgtttt tattctctgc tcttttcttt
    10561 ttacttgctt atttccttac tgtacatcca acacgtttgg tcaaatatgt cggaaagtat
    10621 ttaaactcct tgtttcttgc attattagct atcatttttg tcgtttcttt attccaaccc
    10681 atggggaatc taaaccaatc tgctgcagat tcttatcaga cgaacgcttt gacaaatggc
    10741 gttttagaag gatacaacac ggttgatgca gtagctttac ttgctttaag tgtcactttt
    10801 gtccatgcag ttcgtgatct tggttttaaa gataaaaaag tcacagaatt aacagcaaaa
    10861 gctggtagcc tgagcgttgc gttagaagtc atgatttatt ttggtctggt ccttctcggt
    10921 gcgatgagtt tgaataagat gtctttatca gaaaatggcg gaactgcact ttctcagatc
    10981 actaattttt atttaggtaa cttcggtgct agcttcttag gggtaatggt cacattaggt
    11041 gtcttcacta ccgctatggg actcgttgtt tcttttgcac aagatttcca taaactgttc
    11101 cctaaagtca gttatatgac atggctgcgg ttaacgactt ttgtctcttt tgtcgttgcg
    11161 aatgccggat tggataatat catccaatgg tctttaccag tattgatgct tttgtatcca
    11221 ttgtcacttg cgttaatctt gctttcctta acagcaaaat tcttccaaaa gagtccattt
    11281 gtctatcaag taacgatgtt cttttctgcc gttccagccg tattggatat gttggcaaat
    11341 tcgcctgcat tagtgagtca gcaaactgtc gtagcaagct tactaggatt ttatcatcat
    11401 tatgttcctt ttgctgcatt aggacttggc tgggtcgtcc caacgttgct tggttacgct
    11461 ggcagcttgc tcttttatta tgtatatcgc gtatcaggat ataaacaaga agcaagtgag
    11521 ttaccagaag aataaccaaa tcaaacacaa tttaacataa gagatattaa acaggaaaa
//
LOCUS       DAKBVG010000079        11578 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000079 DAKBVG010000000
VERSION     DAKBVG010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.079"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(99..2225)
                     /gene="pnp"
                     /locus_tag="OUD09_001477"
     CDS             complement(99..2225)
                     /gene="pnp"
                     /locus_tag="OUD09_001477"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCT9692751.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(2413..2682)
                     /gene="rpsO"
                     /locus_tag="OUD09_001478"
     CDS             complement(2413..2682)
                     /gene="rpsO"
                     /locus_tag="OUD09_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCT9692752.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            2933..3496
                     /locus_tag="OUD09_001479"
     CDS             2933..3496
                     /locus_tag="OUD09_001479"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCT9692753.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(3749..4270)
                     /locus_tag="OUD09_001480"
     CDS             complement(3749..4270)
                     /locus_tag="OUD09_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HCT9692754.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(4273..5115)
                     /locus_tag="OUD09_001481"
     CDS             complement(4273..5115)
                     /locus_tag="OUD09_001481"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HCT9692755.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(5108..5833)
                     /locus_tag="OUD09_001482"
     CDS             complement(5108..5833)
                     /locus_tag="OUD09_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCT9692756.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            6009..6305
                     /locus_tag="OUD09_001483"
     CDS             6009..6305
                     /locus_tag="OUD09_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9692757.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            6873..8465
                     /locus_tag="OUD09_001484"
     CDS             6873..8465
                     /locus_tag="OUD09_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCT9692758.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            8478..9218
                     /locus_tag="OUD09_001485"
     CDS             8478..9218
                     /locus_tag="OUD09_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCT9692759.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            9386..10735
                     /locus_tag="OUD09_001486"
     CDS             9386..10735
                     /locus_tag="OUD09_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCT9692760.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(10863..11453)
                     /locus_tag="OUD09_001487"
     CDS             complement(10863..11453)
                     /locus_tag="OUD09_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HCT9692761.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
BASE COUNT         3253 a         2328 c         2278 g         3719 t
ORIGIN      
        1 gactgtgaca cggcttttgt cacagtcccc ttttttcgaa taattattgt ccccctgtca
       61 ccttcacgat cacaagtaca ttattcctat gaattaaatt atttttcttc atttttcgtt
      121 gaaccatctt cgttcaacat tgcttttctt gacagattta cgcgtccttg tttatcgatt
      181 tcagttactt tcacaaggat ctcatcacct agtttaacaa catcttcgac tttattgaca
      241 cgatcgtttg ctaattggga aatatggaca agtccatctt ttcctttgat caagttgacg
      301 aatgcaccga atttttcgat acggacaact ttccctagat aaacttcacc aactttgact
      361 tctttggtca aatcttcgat gatcttgata gcttttttga tcatttctgc atcagctgaa
      421 gcaatactta cttttccatc ttgatcgata tcgattttca caccagtttc atcgatgatt
      481 ccgttgatcg tatcgccgcc tttaccgata acatctttga ttttagcagg ttcgatctgg
      541 atcatttcga tttttggtgc atattggctt aattcttcac gtggagcagc aatcgttgaa
      601 gtcaattctt ctaagatttc catacgtgct tgttttgctt gagttaatgc ttcagttagg
      661 atttgttctg tgattccttg aatcttgata tccatctgta aagcagtgat accatctttt
      721 gtaccggcaa ctttaaagtc catatcgcca agatgatctt ccagcccttg gatatcagtt
      781 aggatcgtat agttttcacc atcagaaaca agtcccatcg caataccggc gactggcgct
      841 ttgattggta cacctgcatc catcaatgct aatgtgccag cacagatact tgcttgggaa
      901 gaagaaccat ttgattccaa tacttctgct actaaacgga tcgtataagg aaattcttct
      961 tctgaaggga ttacttgtgc taacgcacgt tcacctaatg cgccatgtcc gatttcccga
     1021 cgccctggag agcctgcacg tcctgtagaa ccaacagaaa attgagggaa attgtagtgg
     1081 tggatgaatc gtttgctgac ttccacccct aaaccatcaa tgatttggtg ctctcctaga
     1141 ggtgcgagtg tacaagctga taaagcttgt gtttgtcctc gtgtgaacaa acctgatcca
     1201 tgtacacgtg gcaataagct gacctctgat gacaatggac ggatttcatc caatttacgc
     1261 ccgtcaggac ggatcttatc gatggtgatc aattcacgta cgacatcttt ttcaagatct
     1321 tctgtgattt gtttaacttc tttcaacaag tgatcttcat cttcatgacc tgcaaatttc
     1381 tcagcataag cttcgcggat atcgatcttc acctgctcga tattatcttc gcgagcaagt
     1441 ttttcttcag tcattactgc tgctttcatt gtagcgtaag aagcatcaaa gatttctttt
     1501 ttcaatactt catcaacttg cagtaaagtc acttccattt tttctttccc tactgcttga
     1561 acgatttctt cttggaaagc aactaactct ttgatagcgt caaaaccaaa cagcaatgca
     1621 ccaagcatgt cttcttctga aacttctttc gcgccgcttt caaccatatt gatggcttgt
     1681 ttcgttccgg caacagtcaa ttcgatatct gtttgttctg cttgttctac tgttggattc
     1741 aagacgtact cgccatttac acgaccaact tctacgcctg cgattgggcc gtcaaatggg
     1801 atatctgaga ttgatagtgc taaagaagaa cctagcattg ctgccatagc tggcgagcag
     1861 tctgtttcta cactcatcac gatgttcgtt acttggactt cattacggaa gccttctgca
     1921 aacatcgggc gaattggacg gtcgatcaaa cgggcagtca atgttgcttc cgtacttgga
     1981 cggccttctc gtttgatgaa acctccaggg attttccctg ctgcatacat tttttcttca
     2041 taattgatcg ttaatgggaa aaagtctgta tcttttgctt ctttagaggc cacagctgca
     2101 cttaagacta ctgtatctcc ataacggact aaaacggctc cgtttgcttg ttttgctagc
     2161 tgaccgactt cgatttgtaa gggacggccg ccccatgtcg ttttgaatac ttgtttttct
     2221 gacataactt ctccttttcc ttctcgtgaa agcaagacgc tactaaacaa atgaaaactg
     2281 ccgaaagccg tcttttgagc agctttcatt tggttagtag gagtccatct agcttgatca
     2341 cggaatttta cttaattgat atacgagtaa aaaaagtgag attcatgtga atcccactct
     2401 ttttgactaa aattaacgac gcaagcctaa acgtttgatc aattcgcggt aacgttgaac
     2461 gtcagtttta cgtaagtaag ctaacaagtt acggcggtga ccaacttttt tcattaggcc
     2521 gcggtatgaa tgatggtctt ttttgtgcac gcgtgcatgt tcattcaagt ggttgatttc
     2581 ttcagttaat acagcgattt gtacttctgg agaaccagta tccccttcat ggcgagcgta
     2641 ttcgttgatg atttcattct tgcgttcttt tgaaattgcc atttctttca cctctttcga
     2701 ttattcccct gcgactgagt aaaacgttgg tgattcgctt aaccaagtgg caggtcatgt
     2761 gcctagagca cacagctata ctttacctga aaccgggttg aaaatcaagt agttaatcat
     2821 taagaaaagg tagttttcaa acaatcttac tactactttt ccatttttgt ggtaaactat
     2881 aatgcgaaaa tagacttgaa acacagtctt ttaagaaagg ggtacatatt ccatgattac
     2941 aatggatgat atcattcgtg aaggaaatcc gaccttacgc gaagttgcaa aagaagtttc
     3001 tcttccatta agcgaagaag atatctcttt aggaaaagag atgcttgagt tcttaaaaaa
     3061 cagccaagat ccaatcaaag ctgaagaact acacttacga ggcggtgttg gcttagctgc
     3121 tcctcaatta gatatttcta aaagaatcat tgctgttcac gtaccaagtc ctgacccaga
     3181 ggcagatgga ccttctctaa gtactgttat gtataatcct aaaattttga gccattctgt
     3241 tcaagatgct tgtttagggg aaggtgaagg ctgtttatct gttgatcgag aagtaccagg
     3301 atatgttgtt cgccatgcca aaatcactgt gtcttattat gatatgaatg gtgaaaaaca
     3361 caagattcgt ttgaagaatt atgaatcgat cgtcgttcaa catgaaatcg accatatcaa
     3421 cggtgtgatg ttctatgacc acatcaacga tcaaaatcca tttgcactaa aagaaggcgt
     3481 attggtgatc gagtagagaa ttggcaggtt ccttcgatac tatgctacaa gaaagcttta
     3541 taaaaaaggg gagtgctcaa aagctgcact tccctttttt atgtttctcc actaatcgaa
     3601 atttagatag acatctagaa atcgcaattc caaaaaattc tgtgtattgg atacgcatct
     3661 tttcttattt tttcttattt cttccatatg ggtgtttatc tcagagatga tagaatagtt
     3721 tctaggataa aaagtagcag ctacgatgtt atccttcgta gaggattcct gcttcagcta
     3781 gtttttgttt gatgtttttc agcatatgct tttctgggta gcggatcgta tgcagatgga
     3841 tgccgcctgt taattcggat aacaaagtag cattactttt ttgataacgt ctcataaagg
     3901 aatcgatatc ttgtggcgtt ttgatgtgca aagttcctgc taactcaccg taaaggggat
     3961 gttccacaat tacatcaatg atttcaccac cattttcaac gattagtctc agttcttctt
     4021 ctgtttgatc ttttccatgc tggacagcga ttttactgat gtgcccagta ttggtttctt
     4081 ctgtttcgag gatataacct cttgctgttg ccacgatact tttacctgca gctcgtagta
     4141 aagcaatatc gcctacaatg atctgacggc tgactccaaa agttttcgca aacttgctag
     4201 cactcactgg tttttctgta tttgccaagg tttgcatgat ttgttttcgc cgtgtctctc
     4261 cttcgatcac acttattcct ccttcatttt gttttggtat tcgcgcgagt gtttcatcaa
     4321 atcaacatac gtgtctgtta ttgtttcctt atatccctta tttttcagca gactaacaac
     4381 tttatccaat acttgctgct ctctttttgc atcataaact gcttggtttt cttctttttt
     4441 cacttgtcct attttagtta ccgtatccat tcttttttct aataaagcaa ccaactctcg
     4501 atcgatcata tcgatcttat gacgtaatgt ttcaagagac tcttctgtta gttcttcctc
     4561 tactgtcttg atgatacgag caggatttcc tgcaatcact acattgtcgg gaaaagattt
     4621 ggtcacaacc gaacctgcgc cgactactac gttgtcccct agagtgacac ccggcgtgac
     4681 gataactcct ccgcctaacc aaacattatt accgatttta atgggttttg cgtactccag
     4741 accgctgtta cgtttcactg gatgtagtgg atgcgtcgcc gtgtaaagct ggacattcgg
     4801 agcgaacatg cagttatcac cgatttcaat cgtgctgaca tctaaaaatg tacaattgaa
     4861 atttgcataa aaattctccc caacaaatat attatatccg taatcaaaat tgataactgg
     4921 ctccatatag atttttttcc ctgtacggcc aaaagtctct ttcaacaatt gggatcgcag
     4981 ttccgctgat tctgcttggt tgatgatttg gctttgactt cttgcaaaca tacggtcggc
     5041 aaccagctca ggatcattgg caaaataaag ctcgcctgca atcatttttt ctttttcggt
     5101 tttgaattca gtcataatct gctcctaatt cttgtatttt tttgaaaaca accgctccgt
     5161 cttcttgaat aaacgtataa ggctccgcta aataaacagg cgtttgattg attactaaga
     5221 ttttggctga cggctggcga aactgtacca gatcacaaaa cggatgaact tgaaaactcg
     5281 ttccaacaat gacgatcagc tctgcttggc tgacggcata agccgcctgc tccatgactc
     5341 catcttgaaa cccttcacca tataacacga tgtctgggcg tatttgtcct ccgcatgctt
     5401 gatgtttgtc gctgtgaaga tattctttcc agtctaccgt ttgttgacat tttcgacaat
     5461 gacattgata cagactccca tgaaaattga cgagttgcgg gcttttagcc tttgcgtgca
     5521 aaccgtcgat gttttgggaa acaacccaaa catttttttc tttcgctaga tgtgctattt
     5581 ccaaatgaat gatattgggt tttgcttcag gatgataaat ctttttgaca aattgataga
     5641 atttatccgg ttcattcacc attgcttgat gactcagcaa gtattccggc tgctccatcc
     5701 cgtggtagat tcctgacaaa gatcgatagt ctggcactcc agaaggtgtg gatacccctg
     5761 caccggtcaa aaatgtaatt tgctgataag ctaatatctc ttttgctgct tcgtctactg
     5821 ttaattgttt catgttttgt cacctcactt tccattatag cccatactcg aacaaattta
     5881 ttgttgaaaa gtcagaaaaa tgggaataaa aaagtgttat gggcatttga gcaatttatc
     5941 acaatgttca ctgacaaaag aatgaaaacg tgttacaata ctgctgtata aaagtgaggt
     6001 gaatcgatat gttaccattc tttatttatt ggggcgttat tctggcttgt atcgcctggt
     6061 tagcgttaag cgtttatttc tctgtttttt atcttgtacg caaagaaaat ggaaatcttt
     6121 gggcttttgc tttattcaac gtgattgccg caattgtttt agccattaca ttagccgtat
     6181 atcgaacatg gggctgggga atcacccaat actcaagttt gatttatcta atattagcca
     6241 tttatggcgt cgttgttctt ttgcaagcga tcctaggacg tgagccaaag aaagcggcag
     6301 cttaaatgat cgagatggaa ctggacacag gaaattttct gttctagttc tttttttatt
     6361 gtattacata catttttgtg tataattata ctatcgtttg caatggatag atgattcaca
     6421 ttttgattgt tttttcggta aaagtaaaga tttctatatt tatcagtatt ttttcaaaaa
     6481 ccagcagtta tagattgaca aagcctctcg agtatcgtaa actttttttg tataaataac
     6541 tgaatacgag atgcctcaat caatgaggta gaggtcgcga gaatcattaa ccctgtggag
     6601 tgaagcaaac atggagaagc aggaataggg aggatcgccg aaatgtgcac ttttttgctt
     6661 ggagtgtatg ttgggacgca agttaagagc ttgcggactg tcttagcagt gatgctaagt
     6721 tgcgctatgt tttgtgagag gtttatcacc ttatggaatt ttagcggcct ttttatatct
     6781 ataaatcggt cgctttttat tttttcagat aaagctttac cgcatttcat ctagaccgct
     6841 cgtgtatcat ataatcagaa agggagaaaa aaatgaaaaa aaatatggtt tcagttgccg
     6901 tactatttgc ttttgtcttc tcactattca gtgtgggatc tctggcaaat gcagatgaaa
     6961 aaacaccgag cgatgagttt cgcgtaggta tggaggctgg gtatgcaccc ttcaactggt
     7021 ctcaacaaac agatgagaat aatgccttgc caattcaagg ccaaaatagt tacgccggcg
     7081 gttatgatgt tcaaatcgct aaaaaagtag cagatggttt agggaaaaaa ttagtcatcg
     7141 tccaaacgaa atgggatggt ctggctcctg cccttcaatc aggcaagatc gacgcgatca
     7201 tcgccggaat gagccctact gctgaacgac gaaaagaaat tgattttacc gatccttatt
     7261 acgaatcaca attagtagta gtagttcaaa aagatagcaa atatgcaaaa gcaaaaagtt
     7321 tagcagatct ttctggagca aagatcactg ctcagttaaa tacattccac tatactgtga
     7381 tcgatcagat accagatgtg cagaaacaac aagcaatgga taatttctca gcaatgagaa
     7441 cagctttagc ttctggaatg atcgatggtt atgtcagcga acgtccggaa ggtgtaacgg
     7501 caactagtgt gaacaaagac ttggaaatgc ttgaattcag cggtgcagat ggcttccaga
     7561 caaacccgga agatgttcag atttctgttg gtatgcgtaa aggcgacccg gatatccaaa
     7621 aagtaaacca gattctttct ggcatctcta acgatgaacg aaccaaaatt atggaccaag
     7681 cagtcaaaga tcagcctgct gcaactgata gtgaagaaaa acctggcatc atttctgatt
     7741 tcactacgat ttggaaacaa tacggcaata tgttcttacg aggtgctgga cttaccttgt
     7801 tcatcgcatt gatcgggaca gttgtcggta ctacacttgg attactgatt ggtgtcttcc
     7861 gttcggtacc tgaatcagga aatgcactga ctcgcttctt ccaaaaaatc ggaaatgcac
     7921 ttctttctat ctatgtcgaa gtattccgcg gaacaccgat gatggtacaa gcaatggtca
     7981 tcttctacgg tctggcactc gcttttggca tttcattgaa ccgaacgatt gccgctttgt
     8041 ttattgtctc tctcaatact ggagcttaca tgacagagat cgttcgtggg gggatttttg
     8101 cagtagatga agggcagttc gaagctgcgc aagccattgg tatgacccat ggccaaacga
     8161 tgagaaaagt tgttattcca caagttttac ggaatatctt gccagcaaca ggaaatgagt
     8221 ttgttatcaa tatcaaagat acagcagttt taagtgtaat cggcgtagct gatttgttct
     8281 tccaaggaaa ctctgcttcc ggtgcgaatt tccaattctt ccaaacattt acgatcgtcg
     8341 ggatcatcta cttagtcatg acctttacga tcaccagaat cctgcgggcg gtagaaaaga
     8401 aaatcgatgg tccttcagct tataccaagc ttgaagaaat cgacaattca aatttgcagg
     8461 aataaggagg aagaatcatg aatgaaaaaa ttattgaaat caaacatcta agaaaaagct
     8521 tcggtgaaaa tgaagtattg aaagatatca gcgtaacagt caataaaggt gaagttgtga
     8581 cgatcatcgg ttcttccggt tctggtaaat ctactttctt gcgctgtatc aatttactgg
     8641 aaaaaccaac aggcggcgag atcatttata atggagaaaa tgtattagct cctaaatata
     8701 atttacctaa atatcgaacg aatctaggta tggttttcca atccttcaac ctgttcaaca
     8761 atatgaatgt cttggaaaac tgtatgtctg gtcaaacaac tgtcctgaaa cgagataaag
     8821 aaactgccaa aaaagtagca atcgaaaatc tggaaaaagt tggtatggat cgttatatcg
     8881 aagccaaacc agcacaatta tctggtggac agaaacagcg tgtcgcaatt gctcgggcat
     8941 tatcaatgaa tccagatgta ttgctattcg acgaacctac ttcggcgtta gaccctgaaa
     9001 tggttggcga agtgcttaat acgatcaaag acttagcaca tactggcttg acaatgctta
     9061 tcgtgacgca tgagatggaa tttgcaaaag atgtatctga ccgtgtcatt tttatggata
     9121 aaggtgtgat tgcagaagaa ggtacaccag aagatatctt tgtccatccg aaagaagaac
     9181 gtacaaaaga attcctttca cgtattttaa atgcttagga tcaatagctg attgggacaa
     9241 agagctgcgg tgtttaccgc agttcttttt tatgaattac tcataaaagg taaatttctg
     9301 aaaattttgg aatcttagtt gtgtttttca gaaaactctc ttaatatgga agtaattaaa
     9361 cttttttagt taggatgatt taaaaatgaa tgaagagcaa gaactgcagc gtggactgaa
     9421 aaatagacat gtccaactga tttctattgg cggagcgatt ggaacggggt tgtttttagg
     9481 ttccggaaag tctatccagc tagctggccc ttcgatattg ctcgcctatc ttattactgg
     9541 atgcatctgt tttttcatta tgcgtgcgtt aggcgaactt ctgttatcaa acaccaataa
     9601 ccattctttt ctggattttg ttgcagaata tcttggcaaa aaagcggctt tcatcactgg
     9661 ctggacgtat tggttctgct gggtatcgat tgcaatggcc gatttaacag ccatcggaat
     9721 gtatgtacgc tactgggcgc ctggtgttcc tcagtggctt cctgaactga ttgcattagg
     9781 tcttcttctt tgcttgaata tggtggctgt ctcattgttt ggtgagttag agttttggtt
     9841 tgcattgatc aaagttgtag cgatcattgc ctttatcctc gtaggagctt atatgatcct
     9901 cacccactat acgacagata ttggcacagc cagtatcacg aatctatggt ctcatggcgg
     9961 attctttcca actggtgcaa aaggatttat cttagctttc caaatggtaa cctttgcttt
    10021 tgctgggata gaattagttg gattagtcgc tggagaaaca gaaaatcctg aaaaagtgtt
    10081 gccggaagct atcaataata ttcctatcag gatcatctta ttttacttag gatctctttt
    10141 tgtcattatg gcaatctatc catggaacag cttgaatcca gataacagtc catttgtaga
    10201 agtattttcg gaaattggga tcacgattgc tgcttccttg atcaatttag tcgtattaag
    10261 tgcagcagct tctgcctgca acagtgcaat atatagtaca ggacgtatgc ttcgttcttt
    10321 agcgcaagaa ggcagtgccc ctaaaaaatt caaaaagtta acaacacatc atgtgccagg
    10381 gaatgctttg acattttcta ctatcgtcat tttcatttct gttatcttga attacgtgat
    10441 gcctagtgaa gtattcacac tagtaagcag tattgccact acttgtttct tgtttatctg
    10501 gagtatgctt gtctacacgc acatgaaata tcgtaaaagt atcctcggca aaaaagctca
    10561 ttcatttaaa atgccgttgt acccatttag taattatctg gtatttcttt acatggcctt
    10621 tgtgtgtgtc gtcttgttcc taggaaaaga tacgagaatc gctttattac taacacctgt
    10681 gtggttcttg cttctattac taatctatca tatgaaatat gaacgtaaga agtaagaaag
    10741 tagttgtgac agaagtggtc agcttcgaga aataagaagg aatttacgaa aattgcttct
    10801 taaatttttg tgaaatttcg gcttatttct tggagataaa agcttctgtc ccagcctctt
    10861 ttctatttcc ccggaaacaa ccgctgataa taggcatctg cttttttata ggaaaaaatc
    10921 caaataccag atacaaaaaa acgtccgaat aacccgatca acggtacaaa aaagacaccg
    10981 gcaatcaagc ataagttaat catcatggag ataagacttc gacggtcctt tgacaaaact
    11041 ctcacgacat gggaatctgg tttatttgct ttcatgattt gcctcatcaa gataccaaaa
    11101 gaaaaactcc acatcgtgta tactaagaca taagccatct caggccaaaa agcttcaggg
    11161 ttacgcccga cccaactagt aacagaagga cataaagtca gccaaaagag caaaaagata
    11221 ttcgaccaga ccactcggta attaattcgt tctgccatct gaaatatacg atgatggttc
    11281 acccagatca ccgcaacaaa tataaagctg atggtataag ctaataatgt atttcggatg
    11341 acccataaag aatgaaagct agatgtatta gggatcttga tctctaatac aataactctc
    11401 aaaacaatgg ctaccactcc atcagtaaat gcttctaatc tcgactttgt cacgatgatt
    11461 cctcctcaga tgattcagcg cttttatttt atcacttacg ttattaaata accatcgttt
    11521 tatataggga attttctgaa aattgttgca tatttttcga aattttgtta cttttaaa
//
LOCUS       DAKBVG010000080        11363 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000080 DAKBVG010000000
VERSION     DAKBVG010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.080"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(58..285)
                     /locus_tag="OUD09_001488"
     CDS             complement(58..285)
                     /locus_tag="OUD09_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCT9692762.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(388..2976)
                     /locus_tag="OUD09_001489"
     CDS             complement(388..2976)
                     /locus_tag="OUD09_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HCT9692763.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(2996..3655)
                     /locus_tag="OUD09_001490"
     CDS             complement(2996..3655)
                     /locus_tag="OUD09_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCT9692764.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(3704..4207)
                     /gene="trmL"
                     /locus_tag="OUD09_001491"
     CDS             complement(3704..4207)
                     /gene="trmL"
                     /locus_tag="OUD09_001491"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCT9692765.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(4428..5294)
                     /locus_tag="OUD09_001492"
     CDS             complement(4428..5294)
                     /locus_tag="OUD09_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCT9692766.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            5403..6044
                     /locus_tag="OUD09_001493"
     CDS             5403..6044
                     /locus_tag="OUD09_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCT9692767.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(6106..7467)
                     /gene="mgtE"
                     /locus_tag="OUD09_001494"
     CDS             complement(6106..7467)
                     /gene="mgtE"
                     /locus_tag="OUD09_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HCT9692768.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(7485..8384)
                     /locus_tag="OUD09_001495"
     CDS             complement(7485..8384)
                     /locus_tag="OUD09_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCT9692769.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(8386..9183)
                     /locus_tag="OUD09_001496"
     CDS             complement(8386..9183)
                     /locus_tag="OUD09_001496"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HCT9692770.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(9161..9865)
                     /locus_tag="OUD09_001497"
     CDS             complement(9161..9865)
                     /locus_tag="OUD09_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HCT9692771.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            9996..10571
                     /locus_tag="OUD09_001498"
     CDS             9996..10571
                     /locus_tag="OUD09_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HCT9692772.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            10689..>11363
                     /locus_tag="OUD09_001499"
     CDS             10689..>11363
                     /locus_tag="OUD09_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HCT9692773.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         3230 a         2471 c         2040 g         3622 t
ORIGIN      
        1 ttttttcaca tcttctattc aattgtcccg caaaccattt cctcacgatt gaaagaatca
       61 aggattcgca gggctgccag gattcgttgg attgttcggc gtgttaggcg gaaaaatagg
      121 cgccttgtct aacgtttttt ctagttttga ttcttcacga tcgtctcgtt ctacatcagg
      181 actttcattc aatttgaatt ctttttccat ctcatctaac tttttaaagt cattgccaga
      241 ttcttttgct aatttcgcta atttatcttc ggtttcttta gccataaagt aacgcccccc
      301 ttttgatttc agcttagcat atcacactga aaaaacaaaa gaaaacgcta accaacgatc
      361 atttttagct atttctcatc tttaaaatca agcatttttt gccggtataa aatcgtaggg
      421 agaactgttt tccataccaa tcatcggatc tactaactgg ttatcgatca cttctgctgt
      481 aagaacgaaa tctttagaaa cagcaggttc tacctcttct ttcttttctt ctccaacaaa
      541 tgaagaaaac agatctggtt ccactgtttc cattacttgg tttttcgttt cattcgtccc
      601 actgtcatct tctacaggta gcttttcttt tacaacccgt ggctcttttg tatagttccc
      661 atcttcaaat ggatcatttc cgaccaaaga atcttcataa gctgctagtt tggtccggag
      721 agtgatattc attttatcat tatccatgat ccttgatttc aacgtagtca tccggcttgc
      781 tgattcattc gctacacaag tgacataggc ttgctcttcc cctaacaaaa tcaacagttc
      841 cttactgcga gtcactgccg tgtagagcaa attacgctga agcatccgct gatattgatg
      901 gaccatgggc aaaataacca tcttgaattc actgccttgt gatttatgga ttgagcaaca
      961 ataagaaaga gtgattttat tccattcgtt tcttttataa gtgacctcat ttgcatcaaa
     1021 ctggataact aattcatcca ctttatcttc agaatctttt gccaagatga tacccgtgat
     1081 ttctcccata tctccattga atacattcaa ttcgggagta ttgactaatt ggaggacttt
     1141 gtctcctatc cgataaacgg tgtcgttcca atgtacttcc ttttttccag gtttccctgg
     1201 attgaatatc tcctgcatca ttttatttaa cgcatcgatc cctgcaggtc cccgatacat
     1261 tggtgctaat acttggatat cttgtgcacg gaatccctta tttttcgctt tttccacaat
     1321 tttagagatc aagggttcaa tttgataagc atcacttcgg aaataggaac gatctttttg
     1381 attctttgtg aaatcttttg gcagacgtcc ttctttgatc tcatgggcaa gaggaataat
     1441 actagaacca tcaccttgcc gataaatctg attgagttcc attttgggaa tttgttcgat
     1501 ttccaaaaga tcatggagta cttgaccagg accgacagag ggaagctgat ctttgtctcc
     1561 tactaagata acttgcatat tcgtcggaat cgctttgaat aatgtgttag ccagccatgt
     1621 atcgaccata gacatctcat caacaatcaa aagtccgcct tctaattctt tcgtactgac
     1681 tgaaggtgtt ttttcccgtc cattcagccc cagaagacga tggatcgtac tggcaggcag
     1741 acctgtcgtt tcgttcattc gtttagctgc tcggccagtt ggtgctgcaa gtagaatagg
     1801 aaaaatttct tgggtatatt ttgaaggatc caattccagt ccgttcaact ctgcaaaaag
     1861 attgacgatt ccgttgatca cagtagtttt ccccgtacct ggtcctcctg ttaaaataaa
     1921 tagaggggaa cggattgctt cagcgatagc ttgttcttgc gaaggtccat actgtatgcc
     1981 gaatattttt tcgatacgcc gaatattctt ctgcacttct ttttcaggat aatggatttc
     2041 ttttttacga ctgagaaggc gctggatcga attaccgatc ccccattcag aaaagtacaa
     2101 actgttttcg taaagattag tctcttcttg ttgtatcttt ccttcttcaa caagttggat
     2161 aattacagta gcaacctgat caggctggat ttcaattggg cgacttgttt ctaaaatacg
     2221 gatcgtttgc tgaagcaatg tctccgccgg tacgtatgta tctcctgttt gaactgcatg
     2281 ctgaaaaatc tgatgcaaga tagctgctcg aattcttctc ggtgaatctg ctgcaatacc
     2341 gatttgttcg gctagattat ctgcacgttt gaacccgatt ccttctatgt cttcgactag
     2401 ctggtacgga ttttcttcga tgatatccaa agactcgttc ttataggcct ggtagatact
     2461 gaaagctaac tgactaccga acccgtaacg attcagtccc atgatcactt gatccatccc
     2521 gtggtttagc cgaatcgtct cagcaagcat ttctcttttt ggcttcgtca aaccggagat
     2581 ttgttttaat accgaaggat cagcaatgat tttttcgata gcatcttctc ccagcaattc
     2641 tacaattttt tctgctgttt tcttcccgat tcctgggaat ttttcgctgg aaagatacgc
     2701 tacgatccca tttgcagttg ttggcttttc ttgctcatat ctttcgagtt taagctgcct
     2761 tccgtattta ggatgatcca ctaattctcc ataaaaacga taagtctcat tttcttgaat
     2821 atcgccgaaa cttccggtga ctacaatctc tttttcacgg taatcagctg tggtttcact
     2881 gatgtgtacc aaaagcactt tgtaaaaatt actaggattt tgaaaaaaaa tcgcggcaac
     2941 tgtaccaacg aaataaggca tttcttctgg ctgcatcgga agccgcctcc ttcttctata
     3001 gtaaaggttc tggtgctgtt tcagttccta aaaaatctgt ttggttccat actttcagtt
     3061 catatggcaa taatctgcaa ggctcttcag tctctaaaat cgtcaaacta ctattgaaca
     3121 atccacccat ttcacgcaac tcacttagct cttttcctgt catccattga atggctgcag
     3181 ttaaagaagc tccatgtcct acgaataaaa gtgggccatc tttatgtcgg gcaaccgcat
     3241 ctgctacaac agtttcgata cgtgtgattg ccgattcaat cgtttctccg tgaaaaggag
     3301 aaggatcata cagatctaaa tgattacgca aattcggcag attatccgga tatttctggt
     3361 acatttcatc gatcgactga ccttctaaag tacccagccc tagttctctt agcttatctg
     3421 tataaaaaat ctctaccgga cgagcaagct ctttgttgat accttctgct gtatcacgtg
     3481 ctcgctttgc tgtactggaa taaattgctt taaaaggaac atattttacc tttttcccaa
     3541 atgctttgat ttcttcgtaa ctttgtggca aaagcggaga atcgccattg ctcccttgaa
     3601 aacgtctttc ctgattccat tgtgtcttcc catgtcgggt aaaatatagt tccatacgca
     3661 gcaaactcct gtctttcttt tattttactc tataataaac tgtctaatcc agcttgtcat
     3721 tttcatagtg atgcgtcaat tctaaattag ggaatccttg ctggcgaagt gcttcataca
     3781 ctatcattgc agctgtattc gataaattca atgagcggac atgctcatca ttcataggga
     3841 tgcgtaaaca cttttcttcg ttttcacgca taaatgtttc tggcaaaccg gttgtttctt
     3901 ttccaaataa aaagaagtgc tcttctccat cagcaaaatt ttcgtcacta tatactttat
     3961 gggcaaattt tgagatcaaa tgtagtttac gattacctaa aaatgttaaa aaagcttcta
     4021 aattactgtg gtaagtaata tttacatcat tccaataatc caaccctgcc cgttttagat
     4081 gtttgtcatc agttgaaaaa cctagtggct cgattaaatg aagcggagta ttcgtggctg
     4141 cacacgtacg ggcaatattt ccagtgtttg cagggattaa tggttcaaat aaaacgatat
     4201 gattcatcag gatcattcct ctcatattta tgttcgtttc tattttctga caatttgatt
     4261 tttgcataaa aaaaaacgta tcaactcggt gtcagtcact gcgagtcgtt acgttagtga
     4321 agctcttctc actaactttt atcagaacag gaacctgata aaaaccaatg aaaaaacaac
     4381 ttgttgctaa tataacaccc tcaacttttt tttgcaacct ctactcatta aaaactgcta
     4441 gaaaaacgtt ttctcttagt tccttttaat aattctatgt caattgtaat cttcttcaaa
     4501 ggattcttat ataagtctgc tttgattcgc tcttctacct gccattctaa aggtgacatg
     4561 gccaaaagat ctttttgatt ttcctctggt atctcaaatt cataggttac acgctgacgt
     4621 tcagttaatt tcattgcttc agcaaacttg gctactacac gttcattgga ataactctgt
     4681 ttttcaggtt gattcggata aaaagcctga cgtagttcct ttaaataatt ttcttgtggc
     4741 accaccttca acacttctcc atcttctgag agtacacgtt ggaattcttg gtaatgagaa
     4801 ggcgaaaaga tatttaaaac cgcgttgaat gtttgatcag caaacggcaa attggtcaaa
     4861 tcagctacac accaaaatgc ttcggcagat tgttctgtag ctgcgtagac tccttctttt
     4921 gaaatatcaa atccaacagc attttgaatc tttttcaatt ttgaaagttc attcaagaag
     4981 ctaccttctc cgcagccaac atctaatagc gagacttctt caggtaaatg ctgcgcaata
     5041 gtctcaataa cgggctgata catgccagat tggatcattc tacgccttgg aacgaacatt
     5101 ccttgatcgt attctgtttt aaccgcatgg gataaaaaat aaagtgttcc ttttttggaa
     5161 aggtcaaaac ggtgattatt tttgcatatc aagccattat cagacttttt tacttcttct
     5221 tggcaaaccg gacagcggaa cagctgatga ttttctgcca aaaactggcg tcctcggtca
     5281 atttttttta acatagttcc ccctcatttc tgttttatct taccgttaga tgctcacttt
     5341 ggcaactgct aaaaatatga tacaatgcct agaagaaagt gaaagataaa ggagaagatt
     5401 agatgatcaa agaattttgc gctgaaaact acacatctat tcctctagca attgctagag
     5461 gagccgatcg gatcgaattg tgtgacaact tagcggtcgg tggcacaacc ccgagtactg
     5521 gtgtgatcga agaaacgtta agttatgctg gggaaaaaga tatccccgta atgacgatta
     5581 ttcgtcctag agggggcgac ttcgtctata acgatatcga attgaaaatc atgcatactg
     5641 atttgattga agcaaagaaa ttagggacgg atggcgtagt atttggttgt cttactcctt
     5701 caggctggct agacgaagag gccttagaac tattgatcga aacagcagaa gggttgcaga
     5761 tcaccttcca tatggctttt gacagtattc ctgaagatcg gcaattcgaa gcaatcgatt
     5821 ggctggtaga acatggtgtt gaccgtatct tgactcatgg cggtcctgct gacaaaacta
     5881 ttgtagaaaa tttcccacgg cttaaagaat tgattgccta tacagatggc cgcttaatca
     5941 ttttgcctgg tggtggtgtc actgcagaga atgcagcaca tgtagctaaa gaattgaacg
     6001 taagcgaagt acacggaacc aaaatcgtgg atcttcagtt ataatcttat aaacaaaaaa
     6061 acatttgcaa caggcataat gcttattgca aatgtttttg tttttttaca taaaatagcc
     6121 cataaataaa gaagcaatat tgaaataaat cagtacactc gtcaaatcgc tcaaagtcgt
     6181 gataaaggga ccgcttgcta ctgctggatc aaatcctaga cgatccatta gcatcgggat
     6241 taaactacct gcaagattcg ccactgtgat tgcaaatagc atagccatcc ctatgacaaa
     6301 ccccaagata aaattatgtt tccaaaaacc tacgattaga aaaatcgtga tccccgtaac
     6361 agctcctgtg acaacgccgg tcaagacttc agccaaaaca agtcgaacaa atccatgatc
     6421 tttctcatca gaaatcgcaa gtcgacgaac agccacagct agcgactggg ttccagcatt
     6481 accggcagtt cctgtaatca gcgaaatgaa gactgctaaa atactcgctt cacttaccaa
     6541 gtcttcatag tgactgatta aagaagcggt cgacatacct aaaaacaaca gagtgatcag
     6601 ccaaggcaaa cgtcgagacg ctgcctttac tggattttca ttaatttctt ccacattgac
     6661 cccggcaagt cctgaataat cgctagcagc ttcatcatca ataacgtcga tgatatcatc
     6721 gactgtgacg attcctaaca aatgatcatc ataatcggta acaggaagag ctaagaagtc
     6781 ataatctcta aatgtttgtg ccacatcttc ttggtcgtcg cctacatgaa ctgaaagcac
     6841 tcgttcgctc atcagatcag aaatcattgc gtcgtcatca ttcacgatta ggtcgcgcag
     6901 agaaataacc cctactagct gaccttcttg attaacgaca tagatgtaat agattgtttc
     6961 agctacatct gcttcgttct tcagcacgta catcgcagag cgaacagttt gattggctac
     7021 gatcgaaaca aattcagttg tcatgatcgc gccagccgtt tcatcctcgt aatggagaag
     7081 ttccttgatt tcactggcat cgtctgccga catcaaactt aaatatttag caatttgctt
     7141 tttatccagc gtattaagca agtcaacagc attatctgta tacatctcag aaagcatatc
     7201 agctgcatag ctaggacgca tttcatctag gtattctttc atatgttcgt tgtcttcttc
     7261 aatcacatcg aacatatccg ctaattcttt aggcgacaga taggaataga tcagctgcct
     7321 gttctcttct gtaagagatt ggtagaattg cccctgctca tagatatgca tctctaaaaa
     7381 actgtttctg aatgcctgaa tattattttc ccttaaatct tgagtcaact gggcaaatct
     7441 ttcctctaat tcttgatttt catccaaacc aatctcacct cgccttattc ttcaaaccgt
     7501 ctcacaactg cctccatgtc tgcggctaaa ggctgcttga actccagcaa ctcttctgta
     7561 aatggatggt aaaaacgcag atcataacaa tgaagggctt gtctttttat tcccagatcc
     7621 attcgcccgc catacatatc atcaccgagc agggcacagc caattgcgga aaaatgaact
     7681 cttatttgat gtgtccttcc tgtatgcagc tggatatcaa ctaacgcgat ttctgttagt
     7741 cgcttctcta tccagtactc agtctgtgcc gaacgtcctt catcagagac cattcgcttc
     7801 aatagcgaag aataatctcg ggcgatcggc aaatcgatat gtccatgtgc cgataaatct
     7861 gaaagccttc cactgactaa ggcctgatat tttttcacaa acttcttttc acgaagttgc
     7921 atatctaatt ttgcgtgcgc aaatccatgc tttgcaaaaa gcatcaagcc agatgtatcc
     7981 cgatctagtc gcgtcaccac atggattact tggttttcgt aaccttgttt tttataataa
     8041 gcctttacac gattagccat tgtcccattt ggatggtatt gggcaggaat agacgaaacg
     8101 cctgctggtt tatttactac cagaacgtgt tcatcttcat acacgatatc gatcggtgtt
     8161 tcatccaaaa gtacggtttc atgttctcct tcagcaggaa tcacaatggt taccttatca
     8221 tccttagcta gagaaaacaa gacattttct acttgatcat taactttgat ttgaccgcct
     8281 tgaaatttta ttttcgctaa cagacccttt gagatccctt tttcttttaa aaaatatttt
     8341 acttgctgag aatgatcttt ctcataatcc catgtaaact gcatatcaat cctcactaat
     8401 aaaggcatct ttcacacgat gccaaaaatg catatgtcga taagaagcaa aatggatccg
     8461 ttcttctgct attcgataat aaattgagcg gatatttgct tttgcgacat tcaattgatc
     8521 aaccgttaca agataatctt ctgtattttc caattttatt tctagccatt catcatgggc
     8581 aatcacgatc ggagaaccca aagtacgaaa cactcgatta ttcagtgaag caatctctgc
     8641 caactgaaag gcattgatac taggatggat cacagcaccg ccgacacttt tattatatgc
     8701 tgtcgaacca gtaggcgtag aaatcgtcaa tccatctcct ctaaaacttt caaacagctc
     8761 gtttttgatg tatacatcgg ctaccatcgt acgattggcc cgtttaatcg ttgattcatt
     8821 cagtgctaaa aaatgctggt ccggtgtttc atccaaataa ctgatccgaa catccagcaa
     8881 gggataacta acactttgtt cccggttcgt tcgtaagctg tctactagtt cttccagctc
     8941 gtaatcccgc caatccgtgt aaaaccctaa atgacctgta tgcacgccca agaaacgcac
     9001 atcattcaga caatgactgt agtgatggaa cgccgacaat aatgtgccgt ctccgccgac
     9061 tgagatgacc aattctggct catgatcatc tcgttgaatc cctgcttgtt ctaaaagtgc
     9121 gttcaaacgt tctgtcactt ctatagtctt tgcttcttta ttatgaacga tcgctacttt
     9181 catcccctgc ctcctctctt tcgctttctt tcttcaaaag cgttcgatag tagacatcat
     9241 ctgattttcc tcttccatgg gagaataacc gctgtgcttc ttggatttct tcacggatac
     9301 tggacatctc ttcatccaac tgataggcag cttctgccgc acgctgaagt cgttcactca
     9361 tttcttctgg aaaaacgcct tgatatttat aattcagcga atgctcaatc gttgcccaga
     9421 aattcatcgc aagagtccga atctggattt ccgccaaaat tttcttctca ccgctgatca
     9481 gctgtacagg atactcgatc accagatgat aagaacggta gccacttgct tttttatttg
     9541 tgatgtaatc tcgttcttgg agaactttca tgtcttttct tttacggatg atctcaacaa
     9601 cttgatggat atcttctaca aactgacaca tgatccttaa cccggcaata tcagacattt
     9661 cttcttctaa acgtcccatt ggaatatgcc gtaattgtga ttttgttaca atactatcga
     9721 ctggcttgac tctcccagtg acaaattcaa tcgggacatg tcgattttgt tcacggaact
     9781 gtttgcggat cccccgcagt ttcactttca attctgatac agtttgttca tatggtgcta
     9841 aaaatgcttc ccaatctcgt tccatagctg atacctctac ttcattaaca ttcattgttt
     9901 attctaccat atatgcaaag ttttttcgta ctcttgattt atgcattgac caaaaaaaga
     9961 aaattaggca aaatagacaa aaaggagtcg atcaaatgac acaaatgctt gaaatcgaat
    10021 ataaatctat gctgacaaag caagagtacg agatattcat cgctcactat caattaacag
    10081 ataaagattt ccgtgtacag actaatattt atttcgatac cgttgatgaa cagctgaaga
    10141 aacaaaattg tggcctacgg atccgtttcg ttggtcatca agctgaatat acattaaaaa
    10201 cacctgctga aaaagggcgt ctcgaaacaa cggatacctt tataacagaa gaagcagaag
    10261 catttatctt cgaaaaacgt ctgcctcgtt caggcgcagt ttttcaaaaa ctttctgatt
    10321 tgaatatcga tcctgcttca ttaatccaaa ccggaaagct tacaacaaaa cgagctgaat
    10381 tccctattga agaaggactt ttagctattg atgaaagctg gggagacaat ctacacgatt
    10441 ttgaactgga actagaggta ggggatgcat ctgtgggaaa aattgctttt ataaattttt
    10501 taaaacgttt ttctttacct tatcgtcctg ctaagaacaa gatccaacgt atgcttgaag
    10561 ctaaaaatta gaaacctttc attttcacaa ggcacttgag ttcatttttt tacttttttc
    10621 tgttaagtta taggatgtga aagaatctag tcactagttg tatagaatct attttggggg
    10681 agattcatat gatcgaaatc tatttatttg ttaatccttt aggggaatac tgtttcgaaa
    10741 tggaacaaca gttattacaa tttatcgaag aagagtacgg gacaccatca aaagaaaaga
    10801 tgcaatttcg ctttcttcct ttggtcaatt tgcagacaat tggggatgtc atgcaacaaa
    10861 aaggaatttc tcagaacaat cttgaggaac gtaatcactt attctcaatg acttattctg
    10921 cagcattgga ctgcaaagcg gcgcagtttc aaggaaagaa aaaaggccgc cgttttttga
    10981 tcaaattgca agaagcagtc ggctgtaatg acataccata ttctcaagaa cttgctgaat
    11041 ccatatttga agaaatcggc ggcgatttga atatgtttaa agaagatcgt cagtctgact
    11101 tcgttaaaga agtattcttt tctgatcaaa aaattgctcg ggaaatgggc atcacaaaac
    11161 acccttctgc agtcgtttac aattatgcct gcgaccgtga ttttggtgtt ttagtagaag
    11221 taaaaggtac aagtagcctt tggagaatca cagagctgtg taaaacaaca gataactcat
    11281 atcaagtttt taatggtgaa catttacata caaatgattc tcagttccat tcaagaacta
    11341 gccatttaca cttgctttct aat
//
LOCUS       DAKBVG010000081        11227 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000081 DAKBVG010000000
VERSION     DAKBVG010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.081"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            93..575
                     /locus_tag="OUD09_001500"
     CDS             93..575
                     /locus_tag="OUD09_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9692774.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            772..1662
                     /locus_tag="OUD09_001501"
     CDS             772..1662
                     /locus_tag="OUD09_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HCT9692775.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWEIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            1987..4461
                     /locus_tag="OUD09_001502"
     CDS             1987..4461
                     /locus_tag="OUD09_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HCT9692776.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLLKRRNNTVAMTGDGVNDI
                     LAMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLV
                     KNIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNV
                     MEKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKF
                     RSQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLF
                     ERKNEKEQKPFSRVVKKFLSIFKREN"
     gene            complement(4564..6300)
                     /locus_tag="OUD09_001503"
     CDS             complement(4564..6300)
                     /locus_tag="OUD09_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HCT9692777.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNGDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRAYTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLHGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(6297..7001)
                     /locus_tag="OUD09_001504"
     CDS             complement(6297..7001)
                     /locus_tag="OUD09_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCT9692778.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            7132..7635
                     /locus_tag="OUD09_001505"
     CDS             7132..7635
                     /locus_tag="OUD09_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HCT9692779.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            7595..7933
                     /locus_tag="OUD09_001506"
     CDS             7595..7933
                     /locus_tag="OUD09_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HCT9692780.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            7954..9372
                     /gene="ffh"
                     /locus_tag="OUD09_001507"
     CDS             7954..9372
                     /gene="ffh"
                     /locus_tag="OUD09_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCT9692781.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            9484..10062
                     /locus_tag="OUD09_001508"
     CDS             9484..10062
                     /locus_tag="OUD09_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCT9692782.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            10066..10587
                     /locus_tag="OUD09_001509"
     CDS             10066..10587
                     /locus_tag="OUD09_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCT9692783.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(10666..11220)
                     /locus_tag="OUD09_001510"
     CDS             complement(10666..11220)
                     /locus_tag="OUD09_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCT9692784.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
BASE COUNT         3643 a         2050 c         2345 g         3189 t
ORIGIN      
        1 attatctcta aaaaaaaagt ttttccccct aattttttta gatagaaaat tgtatagtat
       61 gatgagagaa aaagtagttt gggggaacgt taatgagaag aaaagcagta atcgatcggc
      121 tttatttatt tttttggttt tttcctatcg ttttgctacc ttttacatgg gtaaatgctg
      181 ttgtacaagg tgcggagata ttaggcctga tccaaggaag cggatttgtg gtttctagga
      241 attttccagc tttgactgtt ttctatttag tgataagtgt aggtctctta ttaccaaaat
      301 ctctgatcaa tcactacaat tggcagctgc tttacttttc ttgcttatta gtatttacat
      361 ctatgttttc tttgctgcct cgcttgatta tcggccctaa tggtttgagc gagagtggat
      421 tgaaggattc agcttattcc tccatgtatt tcttgacgat caaaccatgg ttctatttag
      481 taattgcctc atttattctt gcaacatttt tttacttctg gacagaaatc aggataaaaa
      541 gtgaacgatc tgtatctata aaaaataact attgatggtg gatcatcagt aaaaaagcat
      601 atgagctgcg taaaatgcat gtttctcata tgcttttttt attattatgt aaagaatgcg
      661 aaaaacgttt acatgatttt tacataactt aactatattt ttacagcaac catacgaggg
      721 atttacagtc gactgataaa ctttagttgt aaataagaaa ggagcaataa aatgaaaaaa
      781 gtattgttta ctctaggtat gatgggaatt cttttagccg gatgcggctc aggatcagga
      841 aatacagatg gctctgccac acaaaataat tcaagtgact caaatgaaca agtaaaaatc
      901 gttgcggtag gttcgacagc attacagccg ttagtcgatg cagcacaaga aagctttgta
      961 caagaaaatc caaattatca aatctctgta caaggaggag gaagcggaac tggtttgagc
     1021 caagtcgaag ctggagcagt tactatcgga aactctgatg tatttgcaga agaaagagac
     1081 ggagttgatg cttcaaaatt agtggatcat aaagtagcag tggtcgggat ggcaccaatc
     1141 gtcaataaag atacagatgt caaagatatc accaaacagg aattgattga tatctttaca
     1201 ggaaaaatca ctaactggaa ggaagttggc gggaaagatc aaaaaatcaa cgtcgtgaac
     1261 cgtgcaaacg gcagcggtac ccgcgcgaca ttcgaaaaat ggggactgga tggtgctacc
     1321 cctgtgcagt cccaagaaca agattcatca ggaacagttc ggcagctggt aagtcaaaca
     1381 ccaggagcaa tcagctattt agctttctca tatctcgatg attccacaca agcactaagt
     1441 atcgacggtg tagaaccaaa agaagagaat gtagcagata acagctggga aatctggtca
     1501 tatgaacata tgtacacgaa cggaaaacct tcccctgaag tccaaaaatt cttagactac
     1561 atgatgacag aagaaatcca agaaggacct gtcaaagaat tgggctattt accaatcaca
     1621 atgatggaag tagaacgtga tcacgaagga aatatcaaat aactagcaga taaacatgcc
     1681 acggtagtcg ttactattaa ggaaaaatca actatgtttc cccacatata catttttcct
     1741 ttaattgtga aaaatagttc ctcatatttt gccgtttttt gagctaatac cgagggtcag
     1801 ctgattgaac accgtttatt cgaaaaaaag agctacgaca tagaactgtc gtagcttttt
     1861 tttgctatat gacaggaatg aaaggtgttt gcaagcttaa tgtgaggaac actttccata
     1921 tctgttctat actggatagg agaagtttgg aaatatccag aaaacgaaat caatcgaggt
     1981 agccacatga agaataaatg gataaatcga agtaaaaaaa agaaagtaac acaaagatca
     2041 tcagataaag tcatccgtct ttttccatcg tataaagaag gattaacggc agatcaagtc
     2101 aaggaacgaa tagaaaaagg tgcagcaaat aattcagtcg atcccacatt caaaacaaat
     2161 cagcaaatcg tattggaaaa catatttacg tatttcaatt tgatctttct tatcttagcc
     2221 attttgcttt gtttagtaga atcctataag aacttgactt ttttaccagt tatcatagcc
     2281 aatacaggaa tcgccatttt tcaggaaatt cgatccaaaa agatcttgga cgaactaaat
     2341 gttttaaata cagcaaaagt caatgtcata cgagacggga tagaacaaac aatagatata
     2401 gaagaccttg taatagatga cattgttatt ttagagactg gacatcaaat tccagcagat
     2461 gcagaggtcg tggaagggaa actgcaagtt aatgaggctt tattgacagg cgaagcagat
     2521 gaaataacca aagaaatagg cgatccatta atgtccggca gtttcattgt ttccggaaaa
     2581 gcttacgccc gattagacaa agttggagca gattcgtata tttcccagct gacagtcaaa
     2641 gccaagacaa tgggagaagg agaacagtcg gagatgattg cttctcttaa tcaattgatc
     2701 aaatggattg ggatcatcat tattcctatc ggtatcctgc ttttttctca aagttacttt
     2761 tttaatggaa acacgatcaa agaaagtgtc gttgcaatgg aagcagcatt gatcggtatg
     2821 atacctgaag gactgtactt gctgacgacg attgcattag cattaagtgc gactaaacta
     2881 gcaaagcaaa gagttctttt acacaatatg aaaagcattg agacattggc aagggtaaat
     2941 gtcctttgtg tagataaaac aggaacgatc acagaaaata aaatgtctgt tcaaaaagtg
     3001 atcgtctcta aaaaacaaga aataatcagt cagtcagatc aattggaagc atgcattgct
     3061 gactatgcaa aagcgatggc aggagacaat gcaacaatgg aagctgttaa agcatatttt
     3121 acaaaaacca ctgatcaatc ctatcaccat gttattcctt tttcttctgt ccaaaaattc
     3181 agcagtgtag cgttggcaga caaggtgtat gttttaggag cgcccgaaat ggttttgcgc
     3241 gatcaattgc cggaatatgc ggaagagttt gtttctttcg ctgaaaaagg ctatcgcgtg
     3301 ttggtctttg gtgtgtacga tgggattctt gaagaaccag tattgacaga agcagtcaca
     3361 ccattaggct atattctgat tgctaatcct atcagaaaag aagcaagagc cacatttgat
     3421 tacttcaaaa aacagcaagt agccatcaaa gtgatttctg gagataatcc gctcaccgta
     3481 tccaatgtag ctgtccaagc agggatttca catgctgatc agtacgttga tgcatctaaa
     3541 ttatcagaag aagaatatga agcagctgtc gaaaaatata cagtttttgg acgcgtcaag
     3601 ccagaacaaa agaaaatctt cgttcaattg ctaaagcgtc gtaacaatac agtagctatg
     3661 acaggtgatg gcgtgaatga tattctagcc atgaaagaag cagactgcag tatagcgatg
     3721 gcttccggaa atgaagccgc catgcaggcc tcacaagtgg tcttacttga ttctgacttt
     3781 tcacgcatgc cagaagttgt tgcagaagga cggcgcgttg tgaacaatat tgagcgatca
     3841 gccagcttgt ttttagtgaa gaatattttc tcctttttgc tctcactgtt ttcagtgatc
     3901 tttgctctga cctatccttt agaaccatca caaatcacat tgattagttt gtttactatc
     3961 ggacttccat ctttcttgct tgctctagaa gaaaacaaaa agcgaatcag agggaaattc
     4021 atcatgaatg tgatggaaaa agctgtgcca ggtggactaa cagatatgat cgtagtcggt
     4081 gcgttggtga tctgcggggt gactttgaat ctaaataaaa cagatgtttc tacagcatct
     4141 accatgcttt tgattgcagt agggttcctc gttctctata aaatctgttc accgctgaat
     4201 aaattcagaa gtcagatcat tcttttctgt gctagcggta actttttttc ggtcatcttt
     4261 ttgcataagc tgttttcaat caccggtatc tcagctgtat cacttttgct gttgagcatt
     4321 ctatttttct cggcagattc gatttttagg catttgacca cattggtaga atatttgttt
     4381 gaacgtaaaa atgaaaaaga gcaaaagccg tttagtcgcg tagttaaaaa atttctatcg
     4441 atatttaaac gagaaaattg acgaaaagta tctcaaaaaa gagtaaaaaa gaacaaccac
     4501 tcgtgtatct ttcgaagcag gatgagtcat ccaactttaa aacgcacgag cagttgctct
     4561 tttttacatt ttcggaatgg taacggtgaa agtcgaacct gttccaagat gactgtctac
     4621 agtaatcgta ccatgaagga gttgcacgta gtcttttaca atcgctaacc ctaatccggt
     4681 cccgcctgaa tgccggcttc gcgccttgtc tacgcggtaa aaacgctcaa agatccgttc
     4741 ctgatcctca atgtcgatac ctataccaaa gtcttggaca gaaaacgata gatcatctgt
     4801 tgcctgatag cggataataa tctcgctgtc gctttttgaa tattggacag cattttcaat
     4861 cagatttttg ataattggat aaaggatatc tgttttagta gtaaatggtt cgttctttcc
     4921 aatcaatcga attgtcaacc gtttcttttc gatgattggc tgataatttt gaacgatttg
     4981 ctggaaataa ggttctgtat aaattgtctg gatctcgtaa gaattctcgc catcttttga
     5041 cagctggatg atttcccgaa tcagtttatc taaccggatc gcatcttttt gcatgatctc
     5101 aagaaaagaa gtcagagttt gcgggtcatc ttttgcacca tccagtaaag tctcggtaaa
     5161 accaatcagt gaagtcacag gagtttttaa ttcgtgagag acgttgccaa cgaaatcctt
     5221 ttgtagcttt tctaaacgtc gcactttcgt taagtcatac gccaccccta gaatctgtcc
     5281 ggtaccttct gaattagtaa agtagcgcaa actcaagtct aatgtctgcc gaccttctgt
     5341 aagggtgatc tcctgatgga tcagcggatg ctcaggagtc acttgatgaa tcagctgaat
     5401 gagttttggc tcttgaatga cttctgtgta ccgctgttca ggttgatagc ttaccacgtt
     5461 taaatgtagc tgcatctttg gattaagcag tagtaaacga gaatcagcat catcaatcag
     5521 gaatacgcca atcatcagtt cgttcagtaa cgtatataat tgctcttctg tggtagtata
     5581 cgcccgatac atcttactca tttgttcgct caacgcattg atcgtctgat acaattcttc
     5641 ccattgatcg ggtgtttgca tgattacttc cgcctgatta ggattttgca ccattttttt
     5701 tagtacgggc agcacggttt tcaatggctg gtttttttga tagatcagat aatagatcat
     5761 tcctgcaaga acaaggaaga aagacagaaa gaagaaaaag acccaacggc gaaaactctc
     5821 agttctcggt aaaaaaccgc ttgtcggttc tgcgatacgt aaaatggctt ctaactggcc
     5881 actttttttg actggtaatg cgacatataa aagatcccca ttcaaagtag tacttttgcg
     5941 caaagcgcta cctaggttcc caccattcaa tacggctttg atttcgggac gggagtttcg
     6001 ctgttcattc aatgtttcat tgtttgtgtc aaataagatc tttccagtag catccattaa
     6061 agtaatccgt tcgttcgact gatggacgaa ttcttccaaa gcagttttgt tttgcgctgc
     6121 ttggaggttt tcgacatcta attgccggat cagcagttgt gctttttttg tcaggtagct
     6181 ttcttgctgt tccacgatct gctgttgaaa ataatgggaa atcaattgcc agccaccaaa
     6241 aaacaaacta actaaaagaa taagaaagaa gagacgttca taaatttttt tccatttcat
     6301 cgtttgggtt cctgaaactt atagccaaat cctctaatag tcaccaagta ctttgggtgt
     6361 tttggatctc gctcgatttt ttctcgcaaa tgactaacgt gtacatctac gattcggctt
     6421 tgtcctgcga agtcaaaatt ccaaatgcgg tctagcaatg tgtctcggtc gatcacacgg
     6481 tcttttcgtt tcatgaaata aacaagtaat tcaaattctt tcggtgttag ttcaatcaga
     6541 tggtcgtcta cggtcacttg ataattggtc aaatcagcag tgatttgtcc aatcgataga
     6601 tattcaggct cagcttcatc ttttgattgc ttacgaggtt caattcgtct gaatattgct
     6661 ttcatacggg ctaatacttc gcgaggacta aatggtttgg tcaaatagtc gtcagcacct
     6721 atttctaggc caatgatccg atccacttgg tcatctttag ccgtcagcat caaaatcggt
     6781 gtttcatttt tttcttgacg cagccgctga gtgatctcca tgccatccat cgttggaagc
     6841 atcacatcta aaatgatgaa atcaaatgat tgttctaatg ctaattctaa tccttcgcct
     6901 ccatctgtgg cactggttac cttgtagcct tctttttcca aattgaacgt caagagcgtt
     6961 acaatagaag gttcatcatc aacaactagt atttttttca ttcaaatctc ctttaaatag
     7021 gtttcattct gtttatattt tagcatagaa tcagtacaaa gggctatttt acaaaagaaa
     7081 taaatggtat aataggaagg cactttgctg aatagaaagg aagagcagtc aatgattgga
     7141 atcagtgcat gtttaggcgg tctggcctgt cgctatgatg gacagaaaaa agaagtacca
     7201 gaattgatgg aactagttga aactggagaa gctgtcatgg tctgtccaga agtgatcggc
     7261 ggattaccaa ttcctagaga tcccgctgaa atcattggtg gaaatggatt cgatgtgtgg
     7321 aaagatcaag caaaagtatg gacgacaagc ggcgaagatg taacggaagc gtacaaaaat
     7381 ggcgcgatca aagcctatca aaaattgcag gaaaaacaca ttgatctagt catcatgaag
     7441 gaaaaaagcc cttcatgcgg aacccattcg atttatgacg ggagtttttc cggaataaaa
     7501 aaaaatggtg ccggagtggc tactgcttat tttattcagc aaaaaatgac tgtgctctct
     7561 gatgaagaat ggctgaaaac tagaggtgag cagaatggaa atcgaaaaga ccaatcgaat
     7621 gaacgcgctc tttgaatttt attcgacctt attgacagaa aaacaaatga actacatgga
     7681 attatattat gcagatgatt tttcattagg tgagatcgcg gaagaatacg aagtaagccg
     7741 acaagcagta tatgacaata tcaagcggac aagcaagatc ttggaggatt atgaaaaaaa
     7801 actccatctt ttttctaatt atattgttcg agaacaggta ttggaaaata tgactgcata
     7861 tattacagat aagtacccgg aagataaaaa actacttgaa tatgtacagc aaattcaagc
     7921 aatcgaagaa taaataaaag gaatgaagaa aatatggcat ttgaaagctt aactgaacgt
     7981 ctccagcagg cgatgggaaa aataagaaaa aaaggaaaag tatcagaagc tgacgtaaaa
     8041 gaaatgatgc gggaaatccg ccttgcctta ctagaagccg atgttaactt gcaagtagtc
     8101 aaagatttta ctaagcgcgt acgcgaacgt gcggtagggg cagaagtgct ggaaagttta
     8161 tcacctgcac aacaaatcgt aaaaatcgtt gatgaagaat tgacgatcac acttggttct
     8221 gaaacagcag agttaaataa atcaccaaaa ataccaactg ttatcatgat ggctggtctt
     8281 caaggggcag gtaaaacgac ctttactggt aaattagcca attatttaaa gaaaaatgaa
     8341 aatgctcgtc cgctcctaat cgcaggggac gtatatcgac cagctgcgat cgaccaattg
     8401 aaagtattag gtcaacagct agacgttcct gtatttgata tgggaactga agtcagcccg
     8461 gtagagatcg tccgtcaagg gatggagtta gcaaaagaaa agaaaaacga ctatgtattg
     8521 atcgatacgg caggacgtct gcatatcgat gaaacattga tggatgagtt gaaacaaatc
     8581 aaagaattga cgcaaccaaa tgagattttg cttgttgtcg atgccatgac tggtcaagat
     8641 gccgtcaacg tagctgatag cttcaaccag caattaggta ttacaggggt cgtcatcact
     8701 aaacttgatg gggatactcg aggcggggca gcactatcga tccgctcagt gacaggcgca
     8761 ccaatcaaat tcatcggttc tggtgaaaaa ttgactgact tagaagtatt ccacccagat
     8821 cgtatggcta gccgtatcct tggtatgggg gatatgctga cattgattga aaaagcgcag
     8881 caagactacg acgagaaaaa agcggaagaa cttgcacaga aaatgcggga aaattcattc
     8941 gatttcaatg actttattga acagcttgat caagtgatgg gtatgggccc actggaagat
     9001 ctaatcaaaa tgatcccagg aatgaatcaa gtccctggaa tcgaaaacgt caaggtagat
     9061 ccaaaagatg ttgaacgcaa aaaagcgatg gtttattcta tgacgccagc agaaagagca
     9121 aatcctgatc tgttgaaccc aagccgtcgt cgccgaattg ctgccggttc tggaaacagt
     9181 gttgttgaag tgaatcgaat gatcaaacaa ttcaaggaat caagaaaaat gatgcagcaa
     9241 atgtccaaag gggatatgaa catcccaggt atggaccaaa tgtttggttc aggcgtcaaa
     9301 ggaaaactag gcaaaatggc gatgaatcga atgatcaaga aaaataagaa gaaaaagaaa
     9361 aaacggaaat aagatgaaaa aaacagcttc ggtacagact gaaaatgtct actgccggag
     9421 cttttctatc agcaattttt attctttact aatacaatta ctctaaacga agaggtggca
     9481 cagatgagat gtccgtggag tgaacaaaca gaaagtatgc ggcaatatca tgatactgtc
     9541 tggggtgttc ctgaatatga tgaccaaaag ctgttccgaa aattgatgct tgatattaat
     9601 caagccggat tgagctggca gacgatttta aataaaagtg aagcattcga tgaagcatat
     9661 gatcgattcg atatcaaaaa agtcgcaaat tatggagaaa caaaaatcag tgaactaatg
     9721 gaaaacaaag gaattattcg taaccgaaga aaaatcgaag cagccgtcca aaacgccaaa
     9781 gcagtcttga aaattcaaga agaaatcggc agtttttcag agtatctttg gtcgttttct
     9841 aatggaaaaa tcgttgattc ttcttatcat tcacaagaag aagtcccaac aacaagtcct
     9901 ctttcagatg aattagcaaa agatctaaag aaaagaggat ttaaatttat cggaagcaca
     9961 acaatctatg cttttttaga agctgtcggg atcatcaatg atcaccttga cagttgtttc
    10021 cgcaaagaag aggtcgtacg tatcggcaga aagggcgaat aaagcatgcg aaaaatcata
    10081 ttttatggtg ctataagttt agatggctat ttggcaacga aaacggatga tcttcaatgg
    10141 ctttttgaca ctccaacagg taagcaaacc acttatgaag ctttttatga aacaatcgat
    10201 acgaccatca tgggcagaaa aacttatcag gaagcaaaaa aatatttgga gaccgaagca
    10261 atttatccag agaaaaagaa tttcgttttt tccacccacg cacaattaca attgccagat
    10321 gctgaggtcg taaacgaaga tcctgttgct tttctaaaaa atctacaaat gacagaagga
    10381 aaagatatct gggttgtggg tggcggtcga ttgatcaaac cgctattgga acatcaaatg
    10441 attgatgaat ggtatatcca gattacccct gtattattag gggacgggat tcctttattc
    10501 caaaaaggaa catatgaagc gcgttttgaa atgctagaca cgacacgatt cggtgaattt
    10561 atcgaattgc attatgtacg aaagtagaag taaataaaat aaaaaaggca actatatgca
    10621 gaaatgtcct gtacatagtt gcctttttta ttaccgttat gctttttact ctgttaattt
    10681 gttagaaaaa tctagatacg catctaaaat cgtttggaag aaacttaatg ttccttcaac
    10741 gatattacca ttttcatcaa ttagattcgc aacgtttccg atatatgctt ccggttgctg
    10801 caaggttggc acattcaaaa agacaagtga ttggcgtaga tggtggtttg cgccaaagcc
    10861 gccgatccca cctggtgatg cagtaacgac taaacctggt ttatgatccc aaacactgtg
    10921 tccataaggt cttgagccta catctaaagc atttttcaat actgctggca cagaacggtt
    10981 gtattcagga gtaacgaaaa cgacaccgtc tagtcccctc acttcctcac gaaaacgagt
    11041 ccattcagca ggagcctgtt ctggagtttc gagatcctca ttgtaaaatg gcagatcatc
    11101 gatttttaca aatacacttt cgtacccttc aggaagcaat ttcgcaaaag cttctgctac
    11161 ttttttgttg taagaatctt ttcttaaact accaataaaa aatccaattt tttttgtcat
    11221 acgattt
//
LOCUS       DAKBVG010000082        11134 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000082 DAKBVG010000000
VERSION     DAKBVG010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11134
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.082"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            117..353
                     /gene="secG"
                     /locus_tag="OUD09_001511"
     CDS             117..353
                     /gene="secG"
                     /locus_tag="OUD09_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCT9692785.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            456..1211
                     /locus_tag="OUD09_001512"
     CDS             456..1211
                     /locus_tag="OUD09_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HCT9692786.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            1214..3571
                     /gene="rnr"
                     /locus_tag="OUD09_001513"
     CDS             1214..3571
                     /gene="rnr"
                     /locus_tag="OUD09_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCT9692787.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            3593..4057
                     /gene="smpB"
                     /locus_tag="OUD09_001514"
     CDS             3593..4057
                     /gene="smpB"
                     /locus_tag="OUD09_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCT9692788.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            4359..5078
                     /gene="atpB"
                     /locus_tag="OUD09_001515"
     CDS             4359..5078
                     /gene="atpB"
                     /locus_tag="OUD09_001515"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCT9692789.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            5131..5346
                     /gene="atpE"
                     /locus_tag="OUD09_001516"
     CDS             5131..5346
                     /gene="atpE"
                     /locus_tag="OUD09_001516"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HCT9692790.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            5437..5961
                     /gene="atpF"
                     /locus_tag="OUD09_001517"
     CDS             5437..5961
                     /gene="atpF"
                     /locus_tag="OUD09_001517"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCT9692791.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            5948..6490
                     /locus_tag="OUD09_001518"
     CDS             5948..6490
                     /locus_tag="OUD09_001518"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCT9692792.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            6513..8069
                     /locus_tag="OUD09_001519"
     CDS             6513..8069
                     /locus_tag="OUD09_001519"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCT9692793.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            8083..8985
                     /locus_tag="OUD09_001520"
     CDS             8083..8985
                     /locus_tag="OUD09_001520"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCT9692794.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            9008..10414
                     /gene="atpD"
                     /locus_tag="OUD09_001521"
     CDS             9008..10414
                     /gene="atpD"
                     /locus_tag="OUD09_001521"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCT9692795.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            10428..10850
                     /locus_tag="OUD09_001522"
     CDS             10428..10850
                     /locus_tag="OUD09_001522"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCT9692796.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         3700 a         1966 c         2450 g         3018 t
ORIGIN      
        1 aataacagct gaaagttttg aactttcagc tgttattcca tttatataga gttgtcacgg
       61 acttttatct atgataaagt taaagagacg atatcttttg ggaaggaggc tgcgatatgt
      121 ataatttaat tttgggaatc gtgatcgttt tatcgattgt tatggtcatt gcgattatga
      181 tgcagccaag caagcaaaat agtgcagcga gtgcatttac cggcggtgcg gatcaactat
      241 tcggcaaaca aaaagctcgc ggctttgaag ctgtcatgca gcgatcaact gcagttatgg
      301 gagctatctg gatgatctta ttatttgtcc tagcatattt atcttcgaag tagttgaata
      361 agcctctcgc aaaacggcgg gcggcttttt ttattgcaaa gagaacgctt aagaagaagt
      421 tatgaaagaa actatggtaa aatgaggcga aagaggtgag acgagtgaga caattaccaa
      481 aaccccttta tgcaaaacat ggaaagcgag cggttttgct gcttcacgct tactcaggaa
      541 gtccaaacga tgttcgaatg ctcgcacgat ttttggaaaa agcagattat acagtttatg
      601 cgccgctttt taaaggacac ggaacaatgg agccatatga tatcttgcaa gaaaaagcag
      661 aaagctggtg ggcagacact aaaaaagcca tccatttttt acagtctgag cagttttctg
      721 atattgccgt attaggatta tcaatgggcg gcatctttgc tgtacgagca ctagaagaag
      781 aatcagtgat tggcggcggt tttttctgtt cgcctttgtc tcctgtaaaa acaaacgtgc
      841 ctgaaaactt tgaaaaatac gtacgtcaag tgctgaagac agcaggtaaa tcagagaaag
      901 aaatcaatga aaaagcagtt gcttaccgct cgttagctga acaacaatta atggatatac
      961 aagatcaggc agcaatcgta gaaagtaggt tgtcagatat ccaacagccg atatttttag
     1021 ctcaagctgg gaaagatgaa atgatcgacc ctaatggcgt atttgaaact gcacgcaagt
     1081 tgagccgtca gcgagtgaca cttcaatggt atcctgaaag tggtcatgtg attacagttg
     1141 gtacagcaag acgcgaatta gaaaaagacg tcttggaatt tttagaaaaa ttaccttgga
     1201 atgaggaata aatatgacaa aagaaacgat aaaagagaaa attctctttt tcatggaaag
     1261 cagtcgcaaa cgcagttttt ctatggaaga aatcgctgaa ggtttagggt tccaaaaaag
     1321 cgatgatttt aaattactag tacaaacgat tgctcaaatg gaaagagaac agagtatagt
     1381 tttcaataaa aaaggaaaag tgaaacttcc tttaaaacca atcttgatcg aaggaacatt
     1441 tcgagcaaat gaacgaggat ttggatttgt aacgattgat cccgaagaag atgacatcta
     1501 tatcccaaaa gaagcgactg gttttgcaat ggatggagac accgtagcaa tcgatatcat
     1561 gaagcgagca aatacagata tggatcgcgg agcagagggg aaagtagtcg agattcgaga
     1621 ccgggcgact actcagttag caggggaatt cgttgcttat tcagaagaag aaatacaaga
     1681 aacagatctt tacggcgttg ttatcccgaa agatagaaag ctcaaccaat ttaaagtata
     1741 tgcagcagca gagggagttc gtccagttga cggaagtatt gtgctgatcg aactgaccca
     1801 ttaccctgaa aaagggtatg cgactagttt agaagggata atcaagcaag tgattggtca
     1861 taagaatgac cctggcatgg atatcctctc aattgtcgtg gctaatggta tccctacgaa
     1921 atttcctgat gatgtattgt ctgaagcaga tgaagttcca gatcaaatca gcgaaaagga
     1981 tataactgga cgtagagatt taagaaatca gctcattgtt acgattgatg gagaagatgc
     2041 aaaagatcta gatgatgcag ttacggttaa aaaattagat aacggtaatt attttcttgg
     2101 agtacacatt gcagatgttt cttactatgt gacacaaggc agtcaattgg acagagaagc
     2161 atatgaaaga gggacaagtg tatatttgac tgatcgagta attccgatga tcccgcaacg
     2221 tttatcgaat ggtatctgtt cactgaatcc ccatgttcca aggctgacca tgagttgcga
     2281 aatggagatt gatccaaatg gacaagtagt ctctcatgat attttcccaa gtgtgatcca
     2341 aacaacagaa cggatgacct acacagctgt caatcaaatc ttagaagatc aagacgaaga
     2401 aacgatgcaa cgctatgctc agctcgtacc tatgttccaa gaaatgggtg aattgcaccg
     2461 aatccttgaa acgatgcgaa tcaaacgtgg cgcgatttct tttgaggatc gcgaagcgaa
     2521 aatccttgtt gatgctgaag gacacccaaa tgacatccag ctaagaagca gaggaattgg
     2581 tgaacgcttg atcgaatctt tcatgcttgc ggccaatgaa acggtagctg aacattataa
     2641 taaattaaat ctgccgttta tttaccgaat acatgaacag ccaaaagaag aaaaaatgca
     2701 gcgcttcttt gattttgctt cagctttggg cattctggtc cgaggaacga aaggaacgat
     2761 cacaccaaaa gatttacagc atgtgatcga aggagtagaa gacaaaccag aagctgcagt
     2821 tatcaatact atgttgctaa gaagcatgca acaagcacgc tattcagaag acaattttgg
     2881 gcattatggc ttagcggcag attactacac acactttaca tctcctattc gacgctatcc
     2941 cgatctaatc gtccatcgtc tgatccgttc ttacagtgaa gataaaagtg aaagcaatca
     3001 aagttcgtgg gctgcggaac ttcctgaaat tgcagatcat agttctaaga tggaacgtcg
     3061 tgcagtagaa acagaacgtg aagtagattc catgaagaaa gcagaattca tgatggacaa
     3121 agtgggagaa gaattcgatg ggatcatcag ctctgttgtg aaattcggcc tgtttatcga
     3181 actgccgaat acagtagagg gattgatcca tatcaatgaa ttgaaacagg actacttcca
     3241 ttttgttgaa aatcatttag ctcttgtagg tgaaagaact ggtctgactt ttaaaatcgg
     3301 acagaaagtg cgagtaaaag tagtcaaagc tgatccagat gaacgagcag tagattttga
     3361 acttgtttcc gcagaagagg ttacagcgtt ggaacggcca aaaacacgaa atggaaaatc
     3421 aggaaaacgc gaaggaaaac aagcaggaaa acgcccagaa agaaatggca agaagaacaa
     3481 acgatatagc gatagtaagc aaacgttcgg taagaaaaaa gggaagaagc cattttacaa
     3541 aggtgttaaa aaaaagaaaa agaaaaaata aaatgagttg gaggaaacag ctatgccaaa
     3601 aggcgaggga aaattaattg cacaaaacaa gaaagctcgc catgattatt cgatcatcga
     3661 cacgatggaa gcagggatgg tcttgcaagg aaccgagatc aagtcgatac gaaacagccg
     3721 gatcaatcta aaagatggat ttattcgcgt ccgcaacgga gaagctttct tgcataatgt
     3781 tcatatcagt ccttatgaac aaggaaatat ttttaatcat gatccgttgc gcacgagaaa
     3841 gttattatta cacaaaaaac aaatcatccg gcttgaaaat gaattgaaaa atactggaat
     3901 cactgttgtt cctttaaaag tctatattcg taacggctat gccaaggtat tgattggtct
     3961 ggcgaaaggg aaaaaatctt atgataaacg ggaagatttg aaacgaaaag atatcgatcg
     4021 acaaattgat cgaacattaa aaaatttctc tagataattt cattatcctt tttcgcatat
     4081 tcggtgcttt tgcttttact taatgataaa atttgttagc atttaatctg ataaaacatg
     4141 attatcatag gttttttctt agatcaggag caccgtgtat aaagaaaaat tcgcttcgtt
     4201 tttgactgaa ataaaaaagt gtctaatttt catgaaaatg ttttgaattt tctgtttcat
     4261 ggtggtatga taccgtgtgg ttagaaaccg attggaaaaa accagatacg cacatgaatt
     4321 gattttatgt gaatttcggg gaaaagaggt gaaatggatt ggatgaacgc tcgctaacgt
     4381 tccacattgg accaatttgg tttgatggta cagtttgtat aatggtgtta ctgacctgtt
     4441 tgattgtttt cttcttagct tactttttta caagaaatct taagttgaaa ccaacaggca
     4501 aacaaaatgc gatagaatgg gtcattgatt ttagtcgagg aatcgtaaca gataatctgc
     4561 ctagaaaaga attgagcaat ttccatttgt tggcatttac attgttttta tttgtttttg
     4621 ttgcaaacaa catcggttta gtaacaaaaa tcgttttacc taacgaaaca acactttgga
     4681 aaagcccgac agcagacccg tttgttactt taactttggc atttataatg atcttactta
     4741 ctcacttatt tggggtgaaa aagttaggct tcaaaggata tttcgtaaat tcgttcttga
     4801 aaccatatag ttttatgttt ccgatgaaac tgattgaaga atttacaaac ttattaacac
     4861 ttgctttacg tctttatggg aatatttatg cgggggaagt tttgctaacc ttgattgcaa
     4921 aaatggtaat aagcttaggc tggttctcat tgccgttagc aattccatta gagatggtct
     4981 ggatcgcatt ctccttgttt atcggaagca tccaagcatt tgtcttcgtt actttatcta
     5041 tggtttatat gagccataag attgaagtgg aagaataata ttcgttttaa aaacaaacta
     5101 ctatttatct aatttaggag gaaaacaatt atgaattata tcgcagcagc aatcgcaatc
     5161 atgggagcag caatcggagc cggttatggt aacggtcaag ttatctctaa aacaatcgaa
     5221 tcaatggctc gccaacctga aatgtctggt caattccgta caacaatgtt tatcggggta
     5281 gctctagtcg aagctgttcc tatcttaggg gtagttgtcg ccttgattct agtttttggt
     5341 gtttaattga atcagtatta aggcagaaac aatttggttt gttactgctt ttttacatga
     5401 aaactagaag tacttcaaag aaaggaaggc tcgcctatgc tgaatcaatt ggcaattgca
     5461 gaagttggca atgcgatgtt aggaaatgtc atcgttgtca gcggctcatt tttgatacta
     5521 ctggctctct tgaagcattt tgcttgggga ccaatcagcg atattttgaa aaaacgtgaa
     5581 gacaagatcg ccaatgattt ggattctgca gaacaatctc gcatcaactc agcgaaaatg
     5641 gaacaagaac gcgaacaaca attgttagcc tctcgttctg atgcagctga tatcatcaaa
     5701 aatgcgaaag aaagtggaga attaagccgc caaaatattt tgaaggatgc tcaagaagaa
     5761 gcagctcgtc taaaaagtaa agcccaagct gatatcactg tagaacgtga ttcagcgctg
     5821 aactctgtaa aagacgatgt tgcagaacta tctcttcaaa tcgcggaaaa aatcttgaac
     5881 aaagaattat ctccagaaat gcacgaatca ttaatcaatc aatatatcga aggtctaggt
     5941 tcttcaaatg aaactagata aatatacagt aggaagacgt tacgggaaag ctttatttga
     6001 actggcgatc gattcaaatc aagcagaaga aatctatcaa gatctcgtaa gcttacgtca
     6061 aatctatgaa caagtacctg gattaggtaa tatgttaagt gatgttcgtc tagaacctca
     6121 tgaaaaacga gaaatcatgg acaagcttgt tagtggctat gatggaatcg ttaaaaactt
     6181 tttagaagtt gtttatgcgt ataaccgaat ggatgatctt ttgttcatga tcgaagaata
     6241 tgaacgtcga tatgatgaac atcaaggttt gctgttggga agtgtcacaa cagcagttcc
     6301 actttcagac gaacaacgaa tacaattaga aaagaatgtt gcaaaaacaa tgaactatca
     6361 atcagtcgaa ttaaaacaaa tcgttgattc ttctattatt ggtggcgcga ttgttgaagc
     6421 taaccatcga gtaatcgacg gaagcattcg cacgcaatta gaaaatatgc gtaatcaatt
     6481 aaatagatag tacaaagagg taggtgaatc gaatggccat caaagcagaa gaaattagtg
     6541 ccttgattaa agaacaaatt gaaaattatc aaaacgtgct ttcagtcgaa gaaatcggta
     6601 ccgtaactta tgtaggtgac gggatcgctc gtgcacatgg tttagaaaac gcgatgagtg
     6661 gtgagttgct tgaattttca aatggctcat acggaatggc gcaaaactta gaatcaaatg
     6721 atgtaggtat cattatcctt ggcgatttcg agtccattcg tgaaggagat aaagtaaaac
     6781 gaacaggaaa gatcatggaa gttccagttg gagaggcctt gatcggacgg gtagttaatc
     6841 cgctaggtca gccaatcgac ggactaggtg aaatcgttac tgacaaagct cgtccagtag
     6901 aagcaatggc accaggcgtt atgcaacgta aatctgttaa cgaaccaatg caaacaggtc
     6961 taaaagcaat cgacgctctt gtaccaatcg gacgtggcca acgtgaatta gttatcggtg
     7021 accgtaaaac agggaaaact tctattgcga tcgatacgat catcaaccaa aaaggtcaag
     7081 atatgatctg tatctatgta gcaatcggac aaaaagattc tacagttcgt acacaagttg
     7141 aaacattgaa aaaatatggc gcaatggatt acacaatcgt tgtgaatgcc ggtgcgtctc
     7201 aaccagcgcc gttgctttat atcgcaccat atgctggtac tgcaatgggt gaagagttca
     7261 tgtacaacgg taaacatgtc ttgatcattt ttgatgatct ttcaaaacaa gctgttgctt
     7321 atcgtgaact ttccttgcta cttcgccgtc caccaggtcg tgaagcttat ccaggggatg
     7381 ttttctactt acactcacgc ttattggaac gtgcagcaaa attaagtgat gatttaggtg
     7441 gcggttcaat gactgcctta ccatttgttg aaacacaagc tggagatatc tctgcttata
     7501 tcccaacaaa cgttatttcg atcactgatg gacaaatctt cttagaaagc gacttgttct
     7561 acgcaggtac tcgtccagcg gttgatgccg gactatctgt atctcgtgta gggggttctg
     7621 cgcaaatcaa agcaatgaaa aaagttgctg ggactttacg tctagactta gcaagttacc
     7681 gtgaattaga agcatttaca caattcggtt ctgacttgga tgcagcaaca caagcaaaac
     7741 taaatcgtgg acgccgtaca gtagaaatct tgaaacaaaa attgcatgca ccgctagctg
     7801 ttgaaaaaca agtagtgatt ttgtatgctt tgacacatgg attcttagat agtatccctg
     7861 tagacagtat tttagacttc gaacatgaat tattcgaata tctggatacc aatcattcag
     7921 atatctttga aacaatccgt acaacaaaag atcttccaga agaagaagcg ttgaacagtg
     7981 cgattcagga atataaagac atgttcttag ctacaaaagg aaataattcc tctacagaag
     8041 ataaattgaa atccattcaa aatgcatagt gaggtgagat gaatgggtgc ttcattaaat
     8101 gaaatcaaga cacgtatcgc ttcaacgaaa aaaacgagcc aaatcacgcg ggcaatgcag
     8161 atggtgtctg cttctaagtt gacaaaatct gaagcttctt cacagaagtt tcaggtttat
     8221 gcaacaaaag taagagaaat cgttacgcat ttgactgcta ctcagttaaa tgatattgcc
     8281 tcagaaaatc ctagagggga tatcaattac aacagcatgc tgctcagccg tcctgttaag
     8341 aaaactggtt atatcgtcat cactgcagat ggcggtttag tcggtggata taacagctcg
     8401 atattgaaac aaacgatgtc tattttagaa gaagaccacc aatcacccga tgattacgtg
     8461 atgatcgcaa ttggcggaac tggtgcagac ttctttaaag cacgcggtat caatctagca
     8521 tatgagctgc gcaatctatc tgatcagcca agctttgatg aagtccgcaa aatcgtaaat
     8581 atggcaacaa ctatgtacca aaacgaagta ttcgatgaat tatacgtatg ttataaccat
     8641 catatcaatt cgctaactag tcagtttcgt gtagaaaaaa tgctgccaat ctcagactta
     8701 gatccagaag aagcaactac tttcgaacaa gaatatattt ttgaaccttc aaaagaagaa
     8761 atcttgtctc agttattgcc tcagtatgct gaaagtttga tctacggtgc gatcgttgac
     8821 gccaaaacag cagaacatgc tgctggtatg acagcaatga aaacagcaac tgataatgct
     8881 gctaatatca ttgatgagct gacggtttct tataaccgtg caagacaagg ggctattacc
     8941 caagaaatta ccgaaattgt tgccggagct tcggcactag aatagtattg agattggagg
     9001 aaaaaagatg agttcaggca agattgttca agtaatcggt cccgttgtcg acgtggaatt
     9061 ttctttagac caatccttac cagatatcaa caacgcatta gttgtttata aaaatgacga
     9121 aaataaatca aaagttgttc ttgaagcagc gttagaatta ggggacggag tgatccggac
     9181 cattgcgatg gaatcaacag atggtttaca aagaggaatg gaagtcattg atacaggcaa
     9241 agcaatctct gttcctgtag gtaaagaaac attaggtcgc gtattcaacg tactaggaga
     9301 tacgatcgat ttagaaacac ctttcccaga agatgcggaa agaagcgaaa ttcataaaaa
     9361 agcaccagcc tttgacgaat taagtacaag tacagaaatt ttggaaacag ggatcaaagt
     9421 tatcgatttg cttgccccat atttaaaagg tgggaaagtc ggactattcg gtggtgccgg
     9481 tgttggtaaa accgtactga tccaagaact gatccataat atcgcccaag aacatggtgg
     9541 tatttctgta tttactggtg taggtgaacg tactcgtgaa ggtaatgact tgtattatga
     9601 aatgaaagat tcaggagtta tcgaaaaaac agccatggtg ttcggacaaa tgaacgaacc
     9661 accaggtgca cgtatgcgtg ttgctttgac tggtttgacg attgcggaat atttccgtga
     9721 tgtagaaggt caagatgtac tgttgtttat cgacaacatt ttccgtttca ctcaagctgg
     9781 atctgaagta tcagccttgt taggacggat gccttctgcg gttggttatc aaccaacatt
     9841 ggcaacagaa atgggtcaat tgcaagaacg tatcacatct acgaaaaaag gttcgatcac
     9901 atcgatccaa gcaatctatg tgccagccga tgactacact gacccggcgc cggcaacagc
     9961 attcgcccat ttggatgcga caaccaactt ggaacgtaaa ttaacagaac aagggattta
    10021 tccagcggta gatccattgg cttcttcttc aagtgcttta gcacctgaaa tcgtgggaga
    10081 agaacattat aaagtagcaa cagaagtcca acacgtattg caacgatatc gtgaattaca
    10141 agatatcatt gctatcttag gtatggatga attatccgat caagaaaaag tgttagtttc
    10201 tcgtgcccgc agagtccaat tcttcttgtc acaaaacttt aacgtggctg aacaatttac
    10261 tggtcttcca ggttcatatg tccctgtaga agaaacagtg aaaggattcc gcgaaatctt
    10321 ggaaggcaaa tacgatgatc ttcctgaaga agctttccga agtgttggcc gaatcgaaga
    10381 cgtcgtagaa aaagcgaaaa cactaggcta ctagaaaggg gtgccccatg gatcaatatt
    10441 taacagtaaa tgtggtaact cctgccggtt tggtctatga tcaccatgca gctatcgtcg
    10501 ttgcacgtac gacagctggt gagcttggga ttttgcctaa acatgcacca atcatcgttc
    10561 cattgacgat tgatgaagtt cgtgtaaaac gaacagattc agatacccac gtcgattgga
    10621 tcgctgttaa tggcggaatc atggaagttc gtgataatgt tgtttcaatc gttgctgata
    10681 gtgcggaacg tgaacgtgat atcgatgtat ctcgtgcgga acgtgcaaaa caacgcgcag
    10741 aacgccaaat cgaagaagca aaagcaaaac aagatacgaa cgaattgaaa cgggcaactg
    10801 ttgcgttgca ccgagcaatc aatcgtatca aagtatctaa gcactcataa aatgagatgc
    10861 ggatgtttat ccgtgtttaa gaacagtaaa aagaggttat gacagaagtg gtcagcttca
    10921 agacataaga aggaatttac gaaaattgct tctcaaattt ttgtgaaatc tcagcttatt
    10981 tttcgaagaa gctacttctg gaacaccgta tatcagaggt tgtaaaatca gcgttttgtc
    11041 ctgagtatta gagaaacaaa tggaaaatag ttcctcatat tttgccgttt tttgagctaa
    11101 taccgagggt cagatgattg aacaccgttt attt
//
LOCUS       DAKBVG010000083        11064 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000083 DAKBVG010000000
VERSION     DAKBVG010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11064
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.083"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            4..915
                     /locus_tag="OUD09_001523"
     CDS             4..915
                     /locus_tag="OUD09_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCT9692797.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            939..2411
                     /locus_tag="OUD09_001524"
     CDS             939..2411
                     /locus_tag="OUD09_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCT9692798.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            2462..3073
                     /locus_tag="OUD09_001525"
     CDS             2462..3073
                     /locus_tag="OUD09_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HCT9692799.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            3103..5016
                     /locus_tag="OUD09_001526"
     CDS             3103..5016
                     /locus_tag="OUD09_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HCT9692800.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            5144..8125
                     /locus_tag="OUD09_001527"
     CDS             5144..8125
                     /locus_tag="OUD09_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 42"
                     /protein_id="HCT9692801.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            8122..9783
                     /locus_tag="OUD09_001528"
     CDS             8122..9783
                     /locus_tag="OUD09_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HCT9692802.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            9823..10755
                     /locus_tag="OUD09_001529"
     CDS             9823..10755
                     /locus_tag="OUD09_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HCT9692803.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         3660 a         1781 c         2283 g         3340 t
ORIGIN      
        1 aaaatgatat caaaattaaa acggcagatg tacttttcgc tgccagtcga acggtttttc
       61 acaatattac taatcttatt tactcttagc tgtatttttc cctttatttt cgtaatcgtc
      121 atttcattaa cagatgaaaa agcattacaa ctcaacggtt acgaattatt gccggctcaa
      181 tggagcactg atgcatatca atacttaata caagatggcg gacagttact tcggtcatta
      241 ggcgttacaa ttatgattac agtgattgga acgttgatta ctgtttttat gacgggaacc
      301 tatgcttatg ttttatcaag agcctctttc ccttaccgga agttctttac tttttatctt
      361 ttttttacca tgttatttgc gggagggatg gttccttctt acttagtgat gacgaagatg
      421 cttggcttaa aaaatacaat ttgggctttg attctacctc ttgcttttag cccatacaat
      481 gtcattattt taaggacatt ctttaagaag tcgattccag aatcgattat cgaatccgct
      541 aaaatggatg gctgtagtga gtttcgagtt tttttccaaa tcgttttgcc tttagcgatc
      601 ccaggggttg caacaatagg tttattttca agcttaggtt attggaatga ctggttcaac
      661 gcattgctgt atattgacac caacaaattg attccattgc aatatttgtt aatgaagatt
      721 caaaattcaa tggaattctt agcaaataat aatgacatta cattagcgca gcaacaagca
      781 attcaaaata gccttcctca agaatcaaca cgaatggcaa tggtcgtagt agctacttta
      841 ccgattgcaa ttgtttatcc gttcttccaa aaatactttg ttcaaggatt gacgatcggt
      901 ggagtgaaag aataaaaaag aataggagat gttaaaagat gaaaaagaac atgcagaaat
      961 atttgattgg tggtgcttct gtactaaccc ttggcctatt aggtgcttgt ggtagcagta
     1021 atggcgaagc aaataatgaa acaggaacca caaaagttac ttggtatcag gttggagaag
     1081 aacccaaaga ttttgatgag gtcatgacaa aagtcaacga aaaattaaag gaagaaatca
     1141 atgtagagtt ggatatgcgg ctgattccgg acggggatta tcagcaaaag cttggtgtga
     1201 tgatcaattc aggagaagaa tatgatattt gtttcgtcaa tggcacagat tatgtgaatt
     1261 atgggaacaa aggtgctttc attagtctca acgatcattt ggataaagag ttgaaagatt
     1321 atgcagctga attgaatgaa ggatttattg aaggtggggc aattaatggc gaaacttatg
     1381 gattgccagt caataacgat attgccacaa cggaatattg gacctttaat aaagatttgg
     1441 tagatcaata ccaattggat gtagagaatg ttgaaggtgt agaaacattg gctccagtat
     1501 tagaaaagtt tgcagaaaaa aatactgata cgaatattac gccaatcggt atgacccgta
     1561 ccagtgtctg tattccttat gacaatatta tcggtggtgg tgtaccgcta gcggttgcat
     1621 ttgaaggcga tacacaaaca gttgttaata cttatgagac tccagaaatg atgacttatt
     1681 tgaaggctat gcatgagttt tataaaaaag gatatatttt gaaagatgca gccactgtta
     1741 cgacagatcc atttcgaatt gatagtaaaa actggttttt aggtcgtaac tcaagtggtt
     1801 atcaaccatt agcacaaaaa aaattagaag ctctagcggg acatggatta attttaaaac
     1861 cagctggaaa agcttttaaa acgactggat ttgcaagagt ggcaatgcat gcggtatctg
     1921 cttcaagtaa acatccggaa gcagccttga aattgttgaa ttatgtgaat accaatcaag
     1981 aagtagcgga tttaatggtt tatggaattg aaggaaaaca ctatgatcgc aatgaagatg
     2041 gttcgattac tttaacaaac tctgatgatt attatcaagg cgcttgggca ttggtaaata
     2101 cttatctagt ttcaaaaact tcaccgatta cagaagaaag taaaaaacta gaagaagaaa
     2161 gtaaaaagtt tcaaaatgaa gcaccgatgt ctcctatttt aggtttctct tttgacgctt
     2221 ctgattttcg aacagaaatt gcaagtgtac aaaatatcgt gtcacaatat gaagtacttt
     2281 taagctcggg aacagttgac cctgaacaat atgtaccaga gttgttagaa aaactagaaa
     2341 aagctggatt ggataaaatt caggcatctg ctcaagaaca atacgatgaa tggcgaaaat
     2401 ctaacaaata attttttact aaatgaaaag aatagactaa gcagttagtg gggggattca
     2461 aatgaacgaa aacaaatgga ccaaaggatt taattgggtt tttcaattgt tgttactaaa
     2521 ctttacttat tttatactaa tttgtttagg atttggaatt ttaggttttt tccctgctac
     2581 gatagctttg attgctggcg ttgtgaatcg aaaaaaatat ggcttgcaag agtctgtgaa
     2641 gttcatgagt aaaatctata agaaaaattt tgttagatat aatctatatt gctggtcagc
     2701 aactttgttg ttggccggca atattactac gatctggctt tgttggcagc aaagcgcacc
     2761 attgttttat ttattaagta cattattatc ggtaattaca ctgttcctag tctttgcaat
     2821 gttgaattgg gtcgtgatca aaagtcttgg gttgtggatg atgggagacc gactgattga
     2881 ctatttgctt ttcacagcaa cgatgttgcc aacggtagtc atgcaaggct tggtgcttgg
     2941 aatttatttc cgaatattga tgattttgcc tgtgattttt ctgtttggcg gaacagctgt
     3001 aacggtaata ttgattagta aattaactga aagagcgtta gcaaagttgc cttgtcagat
     3061 acaatcgcac taattttttc acaaaaagga gtatttcttt ctatgaagtt aactgataaa
     3121 caagtagcta atatagcctt tatccagcaa cttgagattc cattgacaca aaaagatgaa
     3181 gaacaaatag acttgatttt tactgacaca aaggctaatg tgcgacaaat caagattact
     3241 aaaaatgagc agcaacgaat ttgcttgtct tattcacatg aagacttgct gtatcgagga
     3301 ttgtttatct gccttccacg ttttttagct caaggaggag agatgccata cattgaagaa
     3361 acctcgatca ttgagaccag ttattcatta gatgcttctc gtaatggcgt gatgaataaa
     3421 gagactctaa aaaaattcat ttcttactta gcgttgatgg ggtacaacac attgtatatt
     3481 tacacagaag atacctatga agtagaagac cttccatatt ttggttacct tcgcggagcg
     3541 tatacaaaag aagaagtgaa tgaaattgtt gaatttgctt ctggttttgg catagaagta
     3601 gttccatcga tccagaccct tgctcacttg acccaatttt tgaagtggta tgctcaacaa
     3661 gaactgatgg atgatcaaaa tactctactg atcggtgaag aaaaggttta tgcggcaatc
     3721 gaaaagatga ttattgcctg caaagaaatg tatcagacag agcgtattca tttgggaatg
     3781 gatgaagcct atcaagcagg tctgggacga tatttaagcc tgtatgggta ttgtgatcgg
     3841 acaactttaa tgattcaaca tatggaccgt gtgttatcaa ttgttgataa atataagatg
     3901 aaaccaatga tttggagtga tttcatttat aagctattag atgaaaccgg taaagatagg
     3961 tattacaatc ctgattttaa actcaaagca ttagcagcta aaggattacc agcaggatta
     4021 acctatgttc attgggacta tggttcagat gaagtttcct cctatgagaa agtaattgat
     4081 aaccatttga aattttgtga cattgatcat tacgttttag ctagtggtgc ccatatctgg
     4141 gggagactag ccccgaatca tgggaaaaca gaagcaattg tttctgcagc tgtaaaagct
     4201 tctaaaaata aaggtgtacg ttctgtgatg ttaactactt ggggagatga agggcaggaa
     4261 acagagcatt ggcattcctt aattggggca ttaaaattag cagattatgt ctattctgaa
     4321 gaagagtgga atgattttga ggaaatctac gatcgtttat taggaacagg tagttatcaa
     4381 ttaatgtccg aattacgata tttcgatgaa gtggatacca ttacaaataa caacttacaa
     4441 atggcttccg tgagtaaact gctactttgg caagatccgt tagttggtat atatgacttt
     4501 catgtagccg ctcataacaa aattgccact agaacaattg gtacttatta tcatgagtta
     4561 gctcagaaat tagatatgaa taaaaattgg aatccaaaac aaccaatttt aacaactatt
     4621 cgtcaacgat atatactttt agctgaaatt ttagcacaaa aggcagaact aggcatctat
     4681 ttacaacact gctgtctggt agaagacctt gcaggattaa tggaattagc gaatcggtgt
     4741 gaagcactag ccaatcgagt aaatgaattg gcatctattc atgaaataat ttggtatcaa
     4801 acatacaaaa cctatggttg ggaagtatta gaacaacgat atgctgcatt agctcatcgt
     4861 ctgaaaaccg ttgcgcgtcg tattaaaaat catcttcagg ggcaagatgc cctagaggag
     4921 cttttggaag aacgattacc gttttgtcaa tatcaagtgc cgcttgaaat ttctggcttt
     4981 aattatcggc aaagctcggt gtcaggctac aattaacgaa ggaatcttcc cgttgaacgg
     5041 accacttgct catctaatgg gaaagtggtt cgttttctat tcataagtag cttaccacta
     5101 actcttttat taagaagaga aaaaaagtag gaagtgagaa aatatgttat cggactatta
     5161 tcaaaatctg tcagttataa gtgtcaatac agaaccatta cgtaattatt atattcctta
     5221 tgcaagtaaa gaagaagcaa aacaagagtt agaaagggaa aaaacgagcc agtttatcga
     5281 tctcaatggt acatgggatt ttcagtatta tccttctgtc caacagttac cggaaaattt
     5341 ttttcaagag gcctttactg aaagctatga tagaatttct gttcctggag tttggcaaac
     5401 agagggattt gacttacctc agtatatcaa tgtatcttat ccaattcctt ttgatccgcc
     5461 ttatgtacct aatgataccc cttgtggtgt atatcggcgt cagtttcaaa ttgagcaaac
     5521 agatgcacgc ttttacttaa atctagaagg agcagatagt aattgttttg tttggataaa
     5581 tagaaacttt gtaggctatg accaagtgag tcatagtaca tctgaatttg atgtgaccaa
     5641 gtatttacag gttggggtta atgaaattca aattctagtg atgaagtggt gtgatggtac
     5701 ctactttgaa gatcaggata agtggcgaat gagcggtcta tttcgtccag tgtatcttct
     5761 tcaacgacca actatgcatc tgacaagtta tcaactacgg acgttgactg atcctaaaaa
     5821 aggaagcgga acattaatgg tttcttttca acgatttact cctattgaag tatcgttacc
     5881 tatttcttac gaattattcc atcctgatgg acgtctcata gcgaaagggg agtgttactc
     5941 ggaacaacaa ataaatttgg atgttgaaga ttgccttttt tggacagcag aaacgcctaa
     6001 attatataca ttacttttaa catgtaatgg tgaatatttt aaagaaaaag ttggctttcg
     6061 agcactgacg atcaagaatc gtaagttatt gctgaacgga acacctatta aattatatgg
     6121 agtcaactat catgacgtga atcagcaagg cacgccagtg atgacccgag agcagttacg
     6181 tgcagatttg ttattaatga aaaaattaaa tgtgaatact gtacgaacgg cacattaccc
     6241 taaatcacca gatttttatg aattggcgga tgagattggt ctgtatgtaa tcaatgaagc
     6301 agatttggag tgtcatggtg tggttaatct atatgatact gaagcgaatt atcaactaat
     6361 tgctaatgat ccgacattcg aaaagccgat tatggaccga gtgatccgtt ctattcttcc
     6421 ttatcaaaat cgaagctcta tcattatgtg gtctatgggg aatgaatctg gttatggagt
     6481 aaattttgaa aaagctcaag ctttagcacg gaaattggat gattcacggt tgattcataa
     6541 tgaacgctcg attgaaccag ttattgacca agtgaatgat tttagtaact tggatgttat
     6601 tagtcaaatg tatcccccac tggaagatgt tcatcgtcat tgtcaaagaa gtgatactga
     6661 aaaacctttt tttatgtgtg aatatagcca cgctatgggc aacggtcctg gagatcttaa
     6721 aacgtacttc gatttgattc aacagtatga ttcctttctt ggcggttgtg tctgggagtg
     6781 gactgatcat gcaatcaata gaggaacttt agaaacgcca tcttatttat acggtggtga
     6841 ttttggtgaa aaatatcacg acggcaattt ttgcgtagat ggtttattga ccccagatcg
     6901 gcaattaaca gtaggtgcat tggagtaccg taatattcat tcgcctattc aactgattgc
     6961 tgtagatgag gagctgcaag aagtaacctt gagcaatata actgctttta cagccggttc
     7021 agcatttaac ctttctcttg aatggtatat agatggaaaa aaaacagttt atgaagtaat
     7081 gaatcctttc atacttgagc ctggcaaaag ttgttcattg cccatcacag tacctacaac
     7141 gattcatggt gctcatacgg ttgctgtttc gctatctgtt tataaaaatg atgagcttta
     7201 tggacagcaa cagctctttc ttaaaaaaga aaaaatgaga ttgccaagaa ctgctcatca
     7261 taatgatgca ccacaaattt cagtaaaagg cacagagctg ataatcaaag gagaatcctt
     7321 tatttatata tatcaattag caaaagctgc gttcacttct gtaaagatca atcaaaagga
     7381 acttttggaa cgtcctactc aattttcaat ttggcgtgca ccaacggata atgatcgtaa
     7441 cttagtaaac aaatggaaag cagctggata tgctgaaatg tctttagaaa tcagaaaaaa
     7501 agactggaaa ttcgtagatg atcatctcct gatcgaaacg tcttatcgag gagttattcc
     7561 aggattacag ccaattttta caatgcacat acagttgaaa ataaatggtg caggcgtaat
     7621 caaccaaaaa gtatcagtgg agaaaaatcc gatttttcca gatttccctc gctttgggat
     7681 ggactggccg ttgaatgata ctttttcgaa ggtttcttat tgtggctatg ggccgtatga
     7741 aagttatcct gataagcact atgctgcgat gtttggtatt tatgagatgg agattgagga
     7801 atttgatcag ccatatatca aaccacaaga atatggtaac cgctggcaat gtgattgggt
     7861 atcaatcaaa aatcatcaag gatctaccat gacggtttat ggaaactcta atttttctgc
     7921 tctgccttat agtcaagctc aattaaccga aaagtcacac cattatgaat taataaaaga
     7981 aaatagaact tggttcaatt taagttataa acaaaatggt atcggctcac acagttgcgg
     8041 tccttattta gaagatcgtt tccgttttat ggaagatact tttgactgga atatagtgat
     8101 tactttcaaa ggagaaaata gatgaaagaa acaaagatca atcaagtcaa aaaaaaatta
     8161 tctcgttatc aagaagttgt ttatcgaaac cattgcttaa aatggtcaaa aaataagctg
     8221 aatgttcgtt ttaatggatt attttatcgt ttcgaacagt tagaagaagt ctggaaaccg
     8281 gtaacgaatt tccaatcatg tcctgtacag ctcataaatg atccaaaaga aacatacgat
     8341 ttatgtataa cacaccttta taaccagcat ttgaaaaatg acggatttta tttaaaggtt
     8401 actaatgaca atcaacttac gattgaatca gcaaaccttc gagggtttca atatgctatg
     8461 gaagccttat tagagagttt tttttcggta ggagatcaat tattattacc ggaattgatt
     8521 ctgaaacatg aaccatcagt gaaaattcga ggaatcattg aagggtttta tggtatcccc
     8581 tggacacatg aaatgcgcct ctctcttttt tcatatatga aagataacca attgaatact
     8641 tttatgtatg cccctaaaga tgatgaatta ttacgtaaaa aatggcgtga actctacgat
     8701 gcgcaagaat tagacaaatt taaggagctt ttatctgctg ctgaggctag caatatcgat
     8761 ttttggtact taattagtcc tggaaatgat attgatatta cgtgtgaaga agacattcaa
     8821 gttcttctga aaaagttaga acaactgatt gagcttggcg tatttcaatt tggtctatta
     8881 atggatgata ttgactatca attaaaaggt gctgctaaac gccgttttcg agagccagca
     8941 ttcgctcatg cctatttagt gaatcgagta gaggaatatc ttagcactcg gttgtccaat
     9001 catgaattag tcatttgtcc tacagaatat gataatcgtc atggttctcg ctatttagca
     9061 acgttaagtc aagaaatacc agaacaatta ccgttctttt ggactggccc aagcacgtta
     9121 gcggcgtcaa tttctacaga agagctgcag gaaatggctt ctgtctatca acggccgatg
     9181 ctgatttggg ataatattcc ggtaaatgat tatttagagg ataaagaact attgttcatg
     9241 agtccatatg agaatcgaac accgaatctt tctaaggaga gataccaggt tacaggagta
     9301 gtttcgaatc caatggctca attagaagct tcaaagttta cgatcaatag tatggctaat
     9361 tacttatgga actgtgagcg ttttgatcct ttggaaactt ggacgtcagt cgttgaaaaa
     9421 gtagttggaa gtaaactaca accagcttat cttaccttaa ccaatgcttt tccaaatcat
     9481 tacacaagca gtctgctttc agatgaagaa ttactgctag ccaaagaccc agaatgggtc
     9541 acaaaagaaa tggctgcttt agtccaagca gtaaaatgga tccagcaaga acaacataca
     9601 tctagattga gaacagaatt ggagccttgg ttacaagcaa tcacagaaag ttggactttc
     9661 tggcaagaat ggcaattaaa aaaagatcca aaagaagcag aagaatggtt ggctaagaaa
     9721 aaaacacacc gcataggacg tgatttagta actgcccatt tagagcaaat tatcaaaagt
     9781 tagatatctt aaaaaataaa aagcaagaaa aaggagaaca aaatggtcaa gagattaatt
     9841 agtgcaaact acagtgatgt caagaaaatg acgtcaaaag agttgaagca atccatcaaa
     9901 gcgagtgaag gacgggtgat tgtttgtgaa acggttgcta atactcttcc acaatctgga
     9961 gaaattacga atgcagaagt tgcgtcagca tttggtgcag atttgattct tttaaatggt
    10021 ttcgattgtt atcgtcctgc agtttttgga ctaccaaatg tgacgattga gaaggttttt
    10081 gatctacagc aaacgattga aaacccgatt cctaaattaa aagaactagt cggtcgtcct
    10141 gtgggaatta atttagaacc cattgcccct aaaacaaata tgatgagtga gaaagtagaa
    10201 atcagccaag gaagagcaag ctctgttgaa acgatacaaa aagcagaaga actaggagtc
    10261 gattttattt gctttaccgg taatccaggc actggagtaa ccaatttaga aattactaaa
    10321 tccgtaaaaa tggcaaaaaa gtatttttcg ggattgatta ttgctgggaa aatgcatagc
    10381 gccggctcag aagagccagt tgtgaataaa gaggcgatta tttcttatgc ggaagcaggc
    10441 gcagatattt tactagttcc ttcagtaggg tctatcccag gctttttaga gcaagaccta
    10501 attgaagcgg ttcggttggc aaaaaaatat gatttgttaa cgatgtctgc aatcggtact
    10561 agtcaggaaa gtgcccgacc agaaacattg cggcaattag ccattatgaa taaaagttgt
    10621 ggtgttgatt tacaacatat tggagatgct ggatacgggg gattggcacc agcagaaaat
    10681 attttcgaaa cctcggttgc aattagagga atgcgacata ccatcaactg cgttagccgc
    10741 tcgatcaatc gctaacaaaa ttaaataaaa aatggttaaa ttgattattt aagtgggaac
    10801 atacttgaga tatcagctaa cttctgtcac tttaaaaaag ggactgtgac agaagtgaaa
    10861 atagacaaaa aatatccgaa caagagacta ggatagcaga aaaactatcg ttttgcaacg
    10921 aggaccaact ggaccgtagg agcaattctc ttattgttcg gatatttcgc ttgttcaagg
    10981 acttatgtct gtagtgccct tttttggtag gaaaaatggt agggaaccat atcattttta
    11041 atgaaaatca agcatttcaa aagt
//
LOCUS       DAKBVG010000084        11052 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000084 DAKBVG010000000
VERSION     DAKBVG010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11052
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.084"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(124..750)
                     /locus_tag="OUD09_001530"
     CDS             complement(124..750)
                     /locus_tag="OUD09_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCT9692804.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFSLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(772..2100)
                     /locus_tag="OUD09_001531"
     CDS             complement(772..2100)
                     /locus_tag="OUD09_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HCT9692805.1"
                     /translation="MEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTCI
                     NIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARFL
                     SNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPEK
                     LVIIGAGYIGLEFASMFNEYGSKVVVLDAHPEFLPREDEDIAQMILEDMTNAGIEFHL
                     GVSVDQVADQESTAAVTFTENGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDRG
                     AIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVPY
                     SVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETDL
                     ILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(2259..2660)
                     /locus_tag="OUD09_001532"
     CDS             complement(2259..2660)
                     /locus_tag="OUD09_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HCT9692806.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(2746..2952)
                     /locus_tag="OUD09_001533"
     CDS             complement(2746..2952)
                     /locus_tag="OUD09_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9692807.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(3047..4549)
                     /locus_tag="OUD09_001534"
     CDS             complement(3047..4549)
                     /locus_tag="OUD09_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HCT9692808.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(4567..5022)
                     /gene="argR"
                     /locus_tag="OUD09_001535"
     CDS             complement(4567..5022)
                     /gene="argR"
                     /locus_tag="OUD09_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HCT9692809.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(5120..5737)
                     /locus_tag="OUD09_001536"
     CDS             complement(5120..5737)
                     /locus_tag="OUD09_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HCT9692810.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            5958..7331
                     /locus_tag="OUD09_001537"
     CDS             5958..7331
                     /locus_tag="OUD09_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCT9692811.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            7406..8518
                     /locus_tag="OUD09_001538"
     CDS             7406..8518
                     /locus_tag="OUD09_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCT9692812.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(8590..10227)
                     /locus_tag="OUD09_001539"
     CDS             complement(8590..10227)
                     /locus_tag="OUD09_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCT9692813.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(10395..11003)
                     /locus_tag="OUD09_001540"
     CDS             complement(10395..11003)
                     /locus_tag="OUD09_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCT9692814.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         2894 a         2335 c         2024 g         3799 t
ORIGIN      
        1 tcttcttaat cgtttaaaaa agactgtgcc aaattatctg acacagccct cactataaaa
       61 ctgttctcat atagctagat gttcattgat tcaagaactg cgaggacaaa agcaattcct
      121 atcttatgat agttgctcta atacttcctc tgttgtcatc acttgatctg caataaagcc
      181 aaagttcgtc aacgctgcat ccattgcttc ttgaccagga gcaccaattg catctgtcgc
      241 taccacaaca ttgaatccac tctctaccaa ttcacgcata tgggaatcgc tacacaagtt
      301 tccgttcatt ccaccaagaa tcactgtgtc gattccattt tttctcaatt ggagcgccaa
      361 atcattgctt tctggtccaa agattttatg aggagagctg ataatcgtat tttcatccaa
      421 atatggtttc aattcttcga tgaaatcagc tcctgaacct tcttcaactg attcatattg
      481 acttttccta gcaaacatct gttgctcaag catcatatgt tctcctgcac cgccaaattg
      541 ccatttatgg tcatgtgggt aaaaataatg aggagaaata aagagtttgt atcctttttc
      601 ttttgcttgt ttcaccattt cgatcatgtt ttcgatcgta ttgtattttt ctaagatatc
      661 ttttgtcaaa ctaaagccac cgcctgctgg tttcaagaac tcgacttgcg gatcagtgag
      721 aactactgct gtatttttat tattgaacat ggtggattct ccattcgtta tttattttaa
      781 tacatcattt aaagcttcac tcattgttgg atgagaatag atttgatctc tcaataacgt
      841 ataaggaagt tttccattca tagctagcga aatcaagttg atgacttcat gagaatcttc
      901 cgcatagata cttgccccta gaatcaagtc agtttcaggg tcgactaaaa ctttgaatac
      961 acctttagga tcttctaata cttgtgcttt agggacaccg cttgtcatat acttgaataa
     1021 tttgtaattg acgccttgtg ctttggcttg tttttctgtt aaacctacat tggataaagt
     1081 cggagtgata aatacactgt aaggcacagc tttccggtta ttcgttgttc gtttattctc
     1141 gcccttcaac tgatccataa tgattcgata atcatctaga gagatataag tgaactgcaa
     1201 tccgcctttg acgtctccaa tcgcccaaat gttgtctgca tctgtacgaa ggtattcatc
     1261 cacagcgatt gctccgcgat ctgttagttt aacatctgta ttttctaatc caagtgatgc
     1321 tgtacttggt tttcttccag ttgcagccaa aacttttgac gcttggattg ttacttcttg
     1381 gccattttct gtaaaggtga cggcagctgt tgattcttgg tctgctactt gatctacaga
     1441 tacacctaga tggaattcga ttcccgcatt tgtcatgtct tctaaaatca tttgcgcgat
     1501 atcttcatct tcacgaggaa gaaattcagg atgggcatca agaacgacaa ctttagatcc
     1561 gtattcattg aacattgatg caaattccaa gccaatatat ccagccccga tgatcactaa
     1621 tttttccggt aattgttcct gatccatcgc ttgcgtagaa tcaatcaagt agcgacttgt
     1681 attcaatcct ttgattggta aaatgattgg ttcagaacca gtattgatga aaatccgttc
     1741 gccttcaaca atcacttttt caccattatt cgtcacttcg atttgatggt tagaaagaaa
     1801 acgtgctgta ccgtccatca ctgtcccagt tgcctcatcg ctgatcatat gataattttt
     1861 ggcgcgaagc atgcccgtca atttttcttt ccgagcaaca gcctctgtaa agtcgattcc
     1921 tctttctcca ttgaaaatca atgatttaga aggaatacag ccgatgttga tacatgttcc
     1981 gccatacata cgtgttgact tttcaacgac taaaaccgat tcgccttttg aagctaacgc
     2041 cttcgctaaa gtcttaccgc cctttccaaa accgatcaca atattttgat atttttccat
     2101 cctttttccc tcgcttcttt ttttacctac cgattggtac aaaaatatga taaccgataa
     2161 ttgtctattc tccaaatcat ctgctttatt tgtcaaaaaa aaccagacaa aaagcggtcc
     2221 tttcacgttt ttagtctggt tcgatttcct ttattcttct aaacaatcgt tacttctaca
     2281 tcgatatttc ctctagttgc tttggagtaa gggcagaatt catgcgcatc ttctgcaatt
     2341 ttttgggctt cttcttcgct gatgccatcg atatgcactt ctaatactac accgatctga
     2401 tagccgccat catctaaatt ataaagagag acacgtgctt ttactgtgga ctctgctgtc
     2461 attcctgctt ctttgatcat atgttgtacc gccccattga aacaagaagc ataacctgct
     2521 gcaaataatt gttctggatt tgttccttct gcatggattc ctggaggagt gattttcata
     2581 tgcatcgagc catttggtgc ctcgacttct ccattgcgcc cgccatggtt gatcattgct
     2641 gtttcgtatg cttttttcat tgtactgctc ctttcgattg accatttgct acttggctat
     2701 aattatagtg tatgacaagt aaggcgcttt ctacaaaaat tctgcttatt cgaataccag
     2761 aatgccgtct tctactattt ttccaaaaaa caaaaaaata ctactcgtga acaacagcgt
     2821 catatttgtt ccaaaataaa tagcaatctt ttctacataa tcttttatat gattgatttt
     2881 aagatacata tataccagaa agttgacccc ataaaccaat actgttaaaa aaataaccat
     2941 attcgttacc attttttcct ccttttgctg gtctccctac cagcactcga tttaaccata
     3001 ccactttttt tgccgtccaa gcaactagcc tttcaaaaag actggtttat tctgctgtta
     3061 atgattcaag atcagaaaaa tgtccgaata attttgctaa ttttactaac gccaacttgt
     3121 tctttccgaa ttcatcattt tctatctgta atactagtaa cggctcatgt aagcttaaac
     3181 ggatcaaaaa ccagccgttt ccgtatggct cttctagatc atatcgaata ccttcttcat
     3241 tttccgggtc gaagttcatc cctttttctt cttccaacag tcgcttcatt tcttctaata
     3301 tcttgtttcc ttgttcgcgt ggttcatcgc tgaacaattt taatcggatt tcttgtactt
     3361 ctacgggctg tttcaactct ttgatttgtt catctaatgt gatacctttt ctttttaatt
     3421 ctggcaaaag catcaggatc tttgcgatga catatgcacc atcatccaga aaatagtttt
     3481 ccttgaatgc agcgtgtccg cttgtttcga tggctagctg tgtatctttc ccaagacggt
     3541 taagttctaa tgctttattg attacatttc gatagccgga aatataacga acttggatgc
     3601 ctcctttttc ttcaatgaat ctttgtagat gagaagaagt aggggaattc gtcacgattc
     3661 ttgttcctgg atgctctttt aatacgattg tacttaatac tgcgatcaaa ttatttcgat
     3721 tgatcactgt tccagaagca ctgacaactg cagaacgatc cacatctgta tcgaaaatga
     3781 tcccaagatc tgcttcattt gctaaaacag cctgcttgat actttccatc gcttctttat
     3841 tatcaggatt tgggatatga ttaggaaatc ggccatctgg ttccaaaaat tgggaacctg
     3901 ttgaatccgc acctaagcta ttcaatactt tttcagcaaa gaatccacca gcaccatttc
     3961 cggcatcaac aatgattttc caaccattta gtggtttgct ttctagtgtt ccaatcccct
     4021 ttttaatttt tgtgactaaa tcttgcgcat aaggtgtcaa aaggtctgct ttttctacgc
     4081 ttccagtttg ttcgcttttt attttttgct gctgtgatag gataaaagca atatcttctt
     4141 tttctgctcc tccttctgag gtaaagaatt ttattccatt ataataatag ggaagatggc
     4201 tagcagtcag cataaccgca gcatcgcacg agaactgttc aaattgagtg gacataaaca
     4261 tagcaggcgt agtagctaat tcgaaatcga tgacatgtat cccttgttct ttcagacttt
     4321 ggatcaacgc ctctttcagt gctggtccgc ttatcctact gtcatgacca atcccaatcg
     4381 tccatttttt atttggatcg gttgatttct tctttttctt tatccagttt gctagtcccc
     4441 agccgatctt ttttaccatg tctgtcgtca agtttacttc atatccttca actggtaatg
     4501 cgattcctcg aatatccgat ccattttgca gcgctttcat atctttcata taaaagtcct
     4561 cctcatttat tccctcattt tttccagctt ttctgcaaat cgctgtgctt cttcttctga
     4621 gcggcaaata ataatgaatg tatctacacc ggccatcgtt ccgacaactt cagggtaagc
     4681 cacttcatct agccaattcg ttaccacatc ggcaccattt cgttcagtca atacgataac
     4741 catgaactga acagactgga tacgcaaaac ctcgcgtcta attgctgtac gcaaacgttc
     4801 ttcatttaaa gaaacagatg gctggttaaa taatgtatag cgtactattt tgttttcatt
     4861 ttgacttttt actaacttca tttctcgaat gtctcgagaa atcgttgctt gagtcacatc
     4921 ggcgcctgct tcatgcagcc gcgcaagcag ctcttcctgt gtcccaatag gttgttctgt
     4981 aatgatctgg cggatcagtg cttgtcgctg tttctttttc atattagaca cctctcaatg
     5041 ttgattatag caaaagataa gtaatatttc ttcatatttt ggtatttttt acttttattt
     5101 tttgttttca ttcaggttgc taaccttttt ctattttaac acgcaaaatc gtcttcattt
     5161 tttcaggtgc tgattttctt ggatcgctga tgtaaatctc tttgtgttct ttagaagctc
     5221 tttcatagcc ttcttttgcg atgaatgctt ctaatttctc aaacgttgct ggttcttcgg
     5281 catagggccc gacgtgtaag atttgggcac aaagaccttc ttccatcttt ttgaatacta
     5341 cttgttcttt caagtcttcc ggaagtttgc tagaacgctg aatggcttct tgtgctatcg
     5401 cttctgtaac aaagtcaggc tgtcggatca tcaattgata agaaaaatgt tcttttttca
     5461 tcactggttc atgcaaatac ttttcttgca gtccccattg tccttccagt ggatagacag
     5521 tatacatctg ataatcagga atcagccaat tttttttagg cgacatgcgt attgcatagc
     5581 tgactgcata tagacaggct actcttcgtt taaagtcttc ctgattcggg tcgccgatcc
     5641 ctctgatgct aaagaatgat tttttaggaa cctctagtat ttgcgggctt tttttcatac
     5701 tatagctctt gtctttacgc cattcaattt tttccatttt tcttcctccg cgaaattcca
     5761 tttagttatt tattatcata cctctttttg agtgacttgg ctaatcgtgt tgacactatt
     5821 tttcagtttg tgctagaata agtgaagtaa ataaatcgta cagataacac gtttcggcgt
     5881 gtcgagagag aggcggtttc tatttttata atagaaatca ctctcttttt ttacgaaatt
     5941 ttttagaggt gatgtaaatg acaaaacaaa aacagctgct tttagtacct gcagtaattg
     6001 caacagggat gatgtctttt gcgggcgtat tgatcgagac ggccatgaat gtaactttcc
     6061 caactcttat cagacagttt gatttaacga ctgcacaagt ccaatgggta acaacgattt
     6121 atcttttgat gatttcgatc attgttccgc tgtctactta tttgaatcgc aattttagtc
     6181 tcaaaaaact ctttttagct tcaaatctgc ttttcttggc aggtataatg atcgatttct
     6241 tctctcctac cttcatggtc ctattgtttg gtcgattgct ccaaggtgca gcgacaggta
     6301 tcgctcttcc gttgatgttc catattatct tgacatttgc ccccctcaat cgacgaggcg
     6361 cgatgatggg tgtcggtact ttgacaacgg ccattgctcc agcaattggc cccacttatg
     6421 gaggcattat gacttcacat tttacatgga atcatatctt cctgtttctt gtgcccgttt
     6481 tgattctttc tttgattatc ggctggtttg ctattcccga gatggcagta caaaaaagtg
     6541 gttatttgga ttggataagc gttttaggaa tttgtctttt gtttagtgga agtctaacat
     6601 ttttgagtac gttgggaaca gtttccagct ggattgcttt aatcatcggc ggtattggct
     6661 ggttcctttt ttactatcga agtaaaaaag tggagcatcc actgatccga ctgtctattc
     6721 tttcaaataa aacattccgc ttgtttttga ttggcttctt agtttatcag tttttattac
     6781 ttggtgtatc ttttgtcttg ccgaatttcc ttcagatcgt tcaaggaagt agtccttttg
     6841 tcgctggatt agcgatgctt cccggcgcag cggttggtgc tttgctttct cctttttccg
     6901 ggaaaatgct ggatcaatat ggtccgaaga agccaatttt tgctgggctg attctttctt
     6961 tgattggttg gctagcatta ttcctactca taggacacgc ttcagtgggt gtattgattg
     7021 cttgtcatgt gttttatatg atcggtatcg gattatctta cagcaatatg atgaccaccg
     7081 gaatgaatgc actgaatgaa gaactacaag gagacgggaa cgcaatgttc aataccttgc
     7141 agcagttttc tggcgctgtg gctacttcat tagtagcggt cattatcaat tacgtccagc
     7201 atcatacttc ccataactat gaagtgtcta ctacattagg atcaaaagca gcattaggcg
     7261 tcttgttgct gttgcttctc gtaagctttg ctcgattcgc ctattatctg ttctttgcta
     7321 aaaaagcgta gattccatat acggcaggtg ttgcatctgc cgtttttttg tgtgaaaatg
     7381 gttgtttgta aaaggaggct tttttatgga aaaaattgcc attatcggtg gcgggatcat
     7441 tggtatgacc ctagccagtt acttagatac atcaaaattc aatgttgctt tatttgatga
     7501 agggacagga caggcaacta aagctagcgc tgggattatc tccccttggt tgtcgaaacg
     7561 cagaaacaaa caatggtatc gtctggctaa agacggcgct gcttttttcc ctaaacttat
     7621 aaaagatttc caacttgatc aaacgattta cgcacaatca gggacacttt tgcttcgacc
     7681 tgaaaaagat ttagctgaat tggctgttct tgccgagaaa cgaaaagaag aagcgccaga
     7741 aattggaaac atcgaatggc tttcacctga acagacatct caagcacttc cgttattgaa
     7801 accagcagtt tcactgcgta tcagtggtgg tggtcgtctg gatggaaaag catatttaga
     7861 acatcttggt cagcgtttag cacaaaaaca aatggagcag atccaggcaa aagtcacttt
     7921 gcataagaag agcgattctt ggctcatcaa gggaccgttt ggagaataca cagctgatcg
     7981 tgtaattttg acacctggcc cggcacttaa gcctttatta caagaattag gctatgctgc
     8041 agatatacgt ccgcaaaaag ggcagctctt agcatttcag acatcttata agatgagtca
     8101 agactggcct gttgctatgc ttgacggaga agctgatttc attcctttca tggatggaaa
     8161 gatcctttta ggtgctaccc atgaaaacga tcaaggctgg gatttggctc ctactagcga
     8221 agcttataag ggattgacta agagtacaga acagttcttg actgatccag atcagataaa
     8281 aaactggcca tacgagttcc gtgtaggtac ccgtgcttat acgtcagatt tttccccatt
     8341 tttcggcgta ctgccagatg atccaagctt actggctgct agcggtctag gctcttctgg
     8401 cttgaccaca ggtccttata tcggctatct attagccaac ttcttaaata ctggtcattg
     8461 gcctacggaa gattatcaaa aggacatctc cacctatatt acgaaaaatc catcatagaa
     8521 aaacagcttt ttcaagtaag gatcccgagc gatattgcgc atggattact tcttgaaaaa
     8581 gctgttattt tagttcaatt tctgaatcaa attctccaaa ctttcttttg cttcatctgg
     8641 caagagatct tttcctatct gagcttcaat gaatcgtaaa cgaatatcaa gtcgttgttt
     8701 caccagattt ttataggacg tatcctcaaa agaaacagga aaatctggta taggtaaata
     8761 gtggatgtct tcaaatggac caaacgactt gaattctgcc gtttgttgca cgattctttc
     8821 aacgattgac aatgtcgttt caggtttgat ttttttatca tcgaaatatc cagtattcag
     8881 gacatagcag gctgtttgct gtttcaaaaa caactctcga aaagcttgat agtcttccct
     8941 aagaggatag acacggaaag gattagcaaa tggttcaatg actagctcat ctagctgtct
     9001 ttttgtagtg atattttccg cgtctgatcg ttttgttgct aatgttgcac caaaagacgc
     9061 tgctaaaact gggtcattga tcttgataat tggcggaagc gaatcatctt tcatgatcca
     9121 aaaaacagca tcgatctttt cgctaagatg atcgactcgg tcgggtgtcg cataacgtga
     9181 tttgactgtc cgcccatttc cgtttcgtat atctcctgtt actaggactt tttctccttt
     9241 acttgtacat gtcacgccca cattctgaca agtaaggaaa gaagaaatgg ccggatcatc
     9301 agacggatag tcttgtgttt tatcaaagta agaaggttcc aaggcagtag caaaaccatt
     9361 ttttcgagaa ataacgaaag catcgtcatg caagatcgaa acagagtgac cttccgtatg
     9421 ttttgctaag gtaatcgttg attttccaga accggacaac ccaaatacag ccatcgtata
     9481 ggtctggtca ttactagcat attgtttcat cccaccgtgg caggcaataa agccattgcg
     9541 atttcccaat gcccaagcaa gcgtcaaagt cgcttttttt aattctccaa agtaacgtaa
     9601 tcctagcaca gccgctgtat tatggagagc atcaaacaaa atgagtccat ttggaaatgt
     9661 ttcatgtgtc cattcgggat cacaaaagat ataaagatct ccctcatcaa aggaaacaga
     9721 cttttgataa gtctctaaga tcttcgaatt cgctggctga aaattcagca aataagaata
     9781 caaattgttc tcaaatcctt ctggaatcag caaatgactt tgcacagaaa attcttcaga
     9841 taggccaacg attacttttg tatggtaaaa ctggcgcttg cgactttgat acacagcttc
     9901 tcttaaaatt ttctcgtatt tgctttgttc cttgtctaag tcctcaaaga tccgccgagc
     9961 acttgctgtt cttcccacaa tgcttccatc attgaatact agtgtcctaa catcagctgg
    10021 taaatctagt tcttcagcat gtgagattgt taaatctgtg atgatcgttc ctggactccc
    10081 ttttgctagt tgataagctg tcttcctatc aaaaacttca tgaacattat taccgtaaaa
    10141 tgtcgtttct acaatcgatc gaatactgga taacagtgga tttccacttg tgatttcgtt
    10201 taattcgaga ctctcttttg tactcatggc tcctcctcat tccctaaaca aaaaaacgtt
    10261 ttctttatta tacattattc gagtttttat ttgtactgct gcgctgtggg aagcaaacga
    10321 gttatttttc acacaaaaaa accgagaact tgaataaaca agtttctcgg tttcatcatt
    10381 ttccttttat cggcttagta ccagccgttt gctaaccaga atgttttagc agcatcccat
    10441 gaaccataac gagatgttac atattgttct gctacacgtt cttgattggc agctgaatag
    10501 tcgccgttta agtatgaagc atctaattga taacggccga tgtaacgtcc gttagtagct
    10561 gtgtatgaac cgcctgattc tttttgtgcg atccattctt ttgcagagct tgaagttggc
    10621 gctgttgcag cagctggggc agcaggagct acttcaactg tttcaacagg tgcaacgtat
    10681 gtatgttctt gcgttgctgt ttcagttgta gatgctgtat tttctgttgt tttgttttct
    10741 gttgttgctg aagaatcatt tgaagttggg attgttaatt gttcaccaac ataaatgcgg
    10801 ttgatgtttg cgatcgaatt tttttcagca attgcatcga tcattgagtt gtctcctgca
    10861 aatttttgtg agatcttaga taatgaatca cctgatttta ctgtataaac ttcatcagcg
    10921 tgagcatttg tagttcctac aaacattgca gtagcaccag cagcaaaagt tgtacctaaa
    10981 acaattttct taaatgaatt catattaaac tccttttttt ctccgttttt tattctttcg
    11041 acgctattaa ct
//
LOCUS       DAKBVG010000085        10878 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000085 DAKBVG010000000
VERSION     DAKBVG010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.085"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            136..1395
                     /gene="lysA"
                     /locus_tag="OUD09_001541"
     CDS             136..1395
                     /gene="lysA"
                     /locus_tag="OUD09_001541"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCT9692815.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1382..2182
                     /gene="dapF"
                     /locus_tag="OUD09_001542"
     CDS             1382..2182
                     /gene="dapF"
                     /locus_tag="OUD09_001542"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCT9692816.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2185..3066
                     /locus_tag="OUD09_001543"
     CDS             2185..3066
                     /locus_tag="OUD09_001543"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCT9692817.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3063..3779
                     /locus_tag="OUD09_001544"
     CDS             3063..3779
                     /locus_tag="OUD09_001544"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCT9692818.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3785..4480
                     /gene="dapD"
                     /locus_tag="OUD09_001545"
     CDS             3785..4480
                     /gene="dapD"
                     /locus_tag="OUD09_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HCT9692819.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4496..5497
                     /locus_tag="OUD09_001546"
     CDS             4496..5497
                     /locus_tag="OUD09_001546"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCT9692820.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5494..6855
                     /locus_tag="OUD09_001547"
     CDS             5494..6855
                     /locus_tag="OUD09_001547"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HCT9692821.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6815..7966
                     /locus_tag="OUD09_001548"
     CDS             6815..7966
                     /locus_tag="OUD09_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCT9692822.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7941..9110
                     /locus_tag="OUD09_001549"
     CDS             7941..9110
                     /locus_tag="OUD09_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HCT9692823.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9285..10664
                     /locus_tag="OUD09_001550"
     CDS             9285..10664
                     /locus_tag="OUD09_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCT9692824.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
BASE COUNT         3619 a         1904 c         2431 g         2924 t
ORIGIN      
        1 tatttttaaa aaaatatttc gaaatatact agacaattca gaaaattttg tttaatatta
       61 ttccaataaa attcaaatct ttatcaaatg aaattccaat aaaaacggaa aaaaagtgaa
      121 aagaggaatg aaaaaatgac agaattgata attggcggag tcacagcaag cacattggca
      181 gaaaagtata agacacctct gtatgtgtat gatgaagaaa agatgagaca aacgatgctg
      241 tcattcaagc gaggctttgt atcgaaacgt tttgatacaa aagtgcttta cgcctctaaa
      301 gcattccaaa cgattgagat gctgcattta gttcgtgaat atggattcgg gctagatgtt
      361 gtcagtggtg gggaaatcta taccgctttg caagctgatt ttcctagaga tactatctac
      421 ttccatggta acaacaagac gccagaggaa ctgatctatg cacttgaaaa tgatttgttg
      481 catattgttg ccgataattt gatggaagtt gaattattag cagaattaag ccaacgctat
      541 ttaaaaaaaa tctgtatcat gcttcggttg aatgtcggag tagaagcaca tactcatgaa
      601 tatatcgtca ctgcgcatat tgattcaaaa ttcggcatga gctatgaaag taaagagtgc
      661 caacaagccc ttcagttgat aaaagaaagc agctatctag agttagaagg atttcatgct
      721 catatcgggt cacaaatttt tgatgttact gcttggttag ctgaaatcga caaattgacg
      781 aattacttaa ccgattttga ccaacctctt tccttgaatc ttggcggagg gtttgggatt
      841 cgttatacac aaaatgatca gccgatccca gtcgaagagg cactaaaaaa actgattgaa
      901 tatacggaag aagctttgga aaaacgaaaa ctgaccattc gtcaattact gatcgaaccg
      961 ggtagaagta ttgttggaga agctggtaca acactataca cgattggttt catcaaaaag
     1021 acaccacata aaaaatatta ctttgtagat ggcgggatga cagataatat ccgtccagct
     1081 ctgtatcagg cagaatacga ttgtgattta gcagcaaaac tgtcggaaga aaaaacagag
     1141 aaagtgacga ttgcaggaaa aatgtgtgaa tcaggcgatg tggtaattaa agaagcttat
     1201 ttgccgaaag cagaatcggg tgatctttta gctgtttatt ccacgggagc ttatggatac
     1261 tcgatgagca gcaactacaa tcgagcgaca cgaccagcag ttgtttttgt caataatggc
     1321 caatcaagac tgattgttcg cagacaagca ttttctgatc tactaagggg ggaagtttcc
     1381 tatgatcctt cataaatata atggctgcgg aaatgatttt atcttattag attacgcaga
     1441 agacacagat tatagtcaat tagccgcttc tttatgcaca aaagaaaaat atgatataga
     1501 cggattgatc gccgtaaaaa tcgatccgtt ggaaatgatc tattacaaca aagatggcag
     1561 ccgcgcgccg atgtgcggaa atggcattcg ctgttttgct cgatacgtaa atgatcagaa
     1621 catgatgaat gaacaggaat tcaacgtaga gacattagca ggcacaagaa aaattacggt
     1681 gttggatccc tcaccttttt attgcagcgt agatatggga gtgccagatg attctccgga
     1741 aaaaatcaaa gctaatcaaa cttatccaat aaaaaaccag gaaatagaga tcaatggtga
     1801 aaaagtaacg atcactagtt tatttttagg aacgattcat accgttgttt ttgttgaaaa
     1861 tgcgcgtgag gaagtctgga gaaaaagagg tgaactgatc tgtcatcatc ctttgttcaa
     1921 agaaaaaacc aatgtcaact ttgtagaagt agtcaatgaa caagaattga tcgtccgtac
     1981 ctatgaaaga ggagtaggtt ggacacttgc ttgcggtaca ggttgctgtg cctcttatgt
     2041 ggttgctaag gaaactggga aaatttcagc agatcaggta gttgttcact tggaacaagg
     2101 agacttgatg atctctggtg aggatgcgat acaaatggct ggcccagcag ttcatgaatg
     2161 gacaagaata ttggaggaat gaagatgata aaaggttcga tcgtggcgct gatcacgccg
     2221 atgaatgaag agggatcagt agattatgct ggtttggaaa aattgatcca attccattta
     2281 gatgaacaaa cagatggatt attagtttta ggtacaacag gagaatcttc tacgctcaca
     2341 caatctgagg aggagcagat ccttcaactg acagtaaaga aagtggctgg ccgtgtgccg
     2401 gtgattgcag gagctggaac gaataatacg aaagaaacga ttgaaaaagc gaagcatttt
     2461 gcttctttgg gggcagacgc acttttggtc atcacccctt actacaacaa aacaagtgat
     2521 gcaggactag ctgctcattt tacagcaatt gcagaagcct caccgattcc gcttattttg
     2581 tataatgttc cttctagaac aggcatgtcg attccaatcc atgtattagt aaatttagca
     2641 gaacatccta atatcattgg tttgaaagaa gcctcaggag atatggctta tgtgatggat
     2701 gccgctcggt tgattggaga agaattcttc ctttacagtg gaaatgatga tttgatccta
     2761 ccagtaatga gtgtaggagg aagcggtgta ataagtgtat gggcgaatat ccaacctaaa
     2821 atagtccatg aattagtaaa agatacgcag gacggaagat ggcagcaagc aaaagaaaag
     2881 caattaaatg cacttgaact gatccatgcc ttgttcagtg agaccaatcc tattcctgtc
     2941 aaagcagcca tgtcattatt agacttacct tccggacctc tacgcctgcc tttagtttct
     3001 ctttcaaaag agaaaaagaa acaattagct cagcttttgt taaaagaaag gacggttgaa
     3061 aaatgaaaat cggactgatt ggcagtggac agatgggaaa tagagtcgaa gaagttgcac
     3121 aagaaagagg agatacggtc cttcttttac ggacggcacc taaagaagca actacgcttg
     3181 cttttacgga actgccagaa ctgcttattg atttttctca tccagataat ttagagaaaa
     3241 tcctttcgta tagcttatca tatcaacttc cgttagtcat tggcacgaca ggttatacac
     3301 aatcacagtt ccaagaaata aaagaacatg ccaaacatgt acctgtgatg tatagtgcta
     3361 atttttcgtt cggcatcatg gtgatgaatc aactaatcaa gcaagcagct gcaatgttgc
     3421 aaggctggca gatcgaactg attgaaaaac atcatagtcg taaaaaagat gcaccatctg
     3481 gtactgcaaa tatgctgctt caaacaatcc aagaagtgca aaagctgcag cccgtataca
     3541 actggcaagg gaaaaaaaga gaagaaaacc aaattggtgt acacagtatt cgtgcaggtt
     3601 ctttacctgg tgaacatgat gtattgtttg ctgctactga tgaaatattt acgatcaaac
     3661 atgaatcttt ttctaatcga atctttgctg caggcgcagt agaagcagcg gactggttga
     3721 aagatcagtc accaggttat tacaaattag aagatttatt aatggacaaa ggggtgtaaa
     3781 aaacgtggaa gctcaagaaa tcatccgcta cattcaaacg gcatctaaaa agacgcctgt
     3841 gaaagtctat ctgaatgtaa ccgagccaat cgaatttaaa gaaagtaaag tttttggtga
     3901 aggaagcagc ttcgttgttt ttggtgacta tgagacgatt gctccggttt tagaagctga
     3961 atcagaaaaa atcattgaaa tcgaaatcga aactgctgca agatacagcg cagttccttt
     4021 gctagatatt aaaaaaatta atgctcgtat cgaaccaggt gctattatca gagatcaagt
     4081 gtcaatcggc aacaatgccg tgatcatgat gggagcgatt atcaatattg gtgcagtaat
     4141 cggtgaaaac acgatgattg atatgggtgc tgtcttaggt ggacgagcga cagtgggcaa
     4201 aaactgccat attggtgcag gagctgtctt agcaggtgtg atcgaaccag catcggccaa
     4261 accggttatc gtagaagacg gcgtactggt aggtgcgaat gcagtgattg ttgaaggtgt
     4321 ccatatcgga aaagatgcag tcgtggcagc aggagcagtg gttttagaag atgtggcagc
     4381 agaaactgtt gtcggtggaa tacctgcacg tgtattaaaa ataagtgatg atcagacaaa
     4441 agaaaataca gcattgattg ctgcgttacg cgaattatag aggagatgga gaaaaatgcg
     4501 cgaaatagat acctgtattg tgggggcaac cggattagtc ggccaaacga tgctcaaagt
     4561 gttagaagaa tatgattttc cagtaggacg attgaaatta ttagcttcaa aacgttcagc
     4621 cggtaaagtt tgtgtattca agaaaaagaa acacactgta caggaattga cagaaacttc
     4681 atttgaagga tatgatttag cgctattttc tgcaggtgct tctgtatcca aagagtttgc
     4741 gccaatcgca cgaagtcagg gactatttgt catagataat tcctcagcat ggcgggaaga
     4801 ttcggatatt ccattaatcg ttccagaggt caatctggaa gactatacat tgaatcggct
     4861 gattgcaaat ccaaattgtt caaccattca agcggtactt ccgcttaaag ctttacaaga
     4921 agcctttggt atccagcgag tcaattattc gacctatcaa gcagtttctg gttccgggca
     4981 aaaaggcatt gaagatcttc atcgtacgca aaatgacgag gaaccagttt tgtatcctta
     5041 taatattagc caaacggtga ttccggaaat cgatcagcct ttggaaaatg ggtatacaaa
     5101 agaagaacag aaaatgataa atgagacccg taagattctg catcagcctg ctttgccagt
     5161 atcagcaacc tgcgtacgtg tgccaatcga aaatggacat ggagtctctg tggcagttca
     5221 actgcaaaaa gagtttacga cggaagaaat tagagagtgt ctcagccagt ttccagggat
     5281 tcaattagaa gatgatctag cagcacataa atatccgacg agcattcttg ctagaggaac
     5341 agatatggtt tatgttggga ggatcagaaa agacacttct ttagagaacg gattgttgtt
     5401 ctatacgact gccgataaca tacgcaaagg agccgctgcg aatgccgtac agattgctgc
     5461 agctttaatg cagaaaatag cggaggtggc aagatgagta atacgctgaa agtagcgaaa
     5521 ttcggcggaa catcgatgtc tcatgaagag caattcaaaa aagtccgtac aattgttctt
     5581 tcagatccca atcgccaagt gatcgttgtt tctgctttag gcaaacgtac gtcagatgat
     5641 gacaaagtaa cagacttact ttatttgatt tatgcttacc ttcagcatca tgttgattgg
     5701 acaccccttt ggaaaaggat ttgtgatcgt ttcattcaag taagagatgt tcttgccctg
     5761 cagttaccta ttgaagatga actagcacgc atacagaaac aactggaaga cgaaaagata
     5821 actgaagatt atttagtaag tagaggtgaa tatctgactg ctcaactgat ggctgagtat
     5881 cttggttacc agtttattga cgcaaaagac ctgattttct ttgactatga tgggaaaatc
     5941 gatcaggaaa aaacagcaca ggaaatcaca aaagcattta aaagatatcc gaaaatggtt
     6001 gttccagggt tttacggggc gaaccctgca ggaaaagaaa aattattggg ccgtggggga
     6061 agcgacatta ccggcgcgat actggcgaag atgttgcaag cggaaagtta tgagaattgg
     6121 acggatgttt caggaatcat gatggctgat ccgcgaatca tcgatcatcc taaaatcatc
     6181 gaggaaatct cttttagaga actgagagag atggcttata tgggggctaa cgttcttcat
     6241 gaagcagctg ttttcccagt tcaagaggct gatatcccca tccaaataaa aaataccaat
     6301 gatcctttcg ctcaagggac aaaaatatca aatcacgaaa tagaaaagga aaatggactg
     6361 acaggaatag ctggacgcaa ggattttctt tccatcactt tattcaaacg tcatatgtcc
     6421 gatgaaatcg ggtttatctg gaaagcaatg agcatttttg ctaaacatga gatatcaatc
     6481 gaacatattc cttcagggat cgacaatatc ggagtgatcg tctcagcaga agcgattgcc
     6541 gataagttat ttctcatcac gaaagaatta aaagaaacac ttggcgtgga agaaatcgtc
     6601 gtgatcgaag acttagcttt gatctctgtc gttgggggtc cgcataaaga attgattgga
     6661 ttatctggga aagtgttatc gatattgaac aaattagaaa ttcgcacatc gatcttatca
     6721 caaggagcac aggagttgaa tctgatcatc ggtgtaccga ataatcaata tgaaacagta
     6781 gtgaagggta tctatgaagg gatggtgaat acggatgctg aaagaatatc gaatggcact
     6841 ccatcaaatt cctgaaatcg ggtttcatga attcaagaca aaaaaatatt tatatgaaca
     6901 agtaaaaaac agcgaggccg ttatccatga aatcggcgaa acaggactac tcctttattt
     6961 tgataatcag caagaagaaa cgattgcttt tcgaacagat atcgatgcac tgccagtcaa
     7021 agaagaaacg aatctctctt ttgcttctaa gcatgaaggt tatatgcatg catgtggtca
     7081 tgacggacat atggcgatgc ttttagggtt gactgacttt ataactaaac acattgagga
     7141 tcttatttat aatgtagtac tgatctatca gccatcagaa gaaatttccg gtggcgcaca
     7201 aagcgttatt cagtcaggat tgttggataa atatgaagta caagcaatat ttggctttca
     7261 tctatggcct ggactagcta agggagaagt tttctctcga ccaggacctt tgatggcaca
     7321 aagcagtgaa acagacatta tcattaaagg ccagtctgca catattgctt ctagtgaaaa
     7381 aggaatcgat agtctggagg cagctgtacg atttttgaag aatgtttatg attttgaccg
     7441 gcaattaccg gcagagtgga atcatctaat gaaatttggc caaatccaag gtggaacgat
     7501 ccgaaatgtt ttagctggag aagttattgt atccggaagt atccgttctt acagttcaga
     7561 tatgcaaaat tatttgaaga ggggattaga ccatttagca gaacagtttc actctacagc
     7621 tactatttct tttcgctata atgacggcta tccagctgta atcaaccata gaggcttata
     7681 tgcagcactg ttagattcaa acaaactaca tgaattagat gctccagttc ttcaagccga
     7741 agattttggc atatatacgc aacaatatcc atgtgtattt ttctttttag gtgtcggtga
     7801 tacccctgct ttacatgatc cgacgttcga ttttgatatg gatgttttag aaaatggtgt
     7861 agaatggtac aaaacgattc tttttacaga aaatcttctt gtaaaaatcg atagcagttc
     7921 aaaagaggag cgagtaaata atgatgaatt tatccaatcg atttagtcct aggctttcta
     7981 gaatagaagt atcaaaaata cgacaattcg atcagcaaat atcgtcgata ccagatgtga
     8041 tcaaactcac cctgggagaa ccggattttc caactcctga acatgtcaag caagcaggaa
     8101 tagcagcgat tgaagaagac ttttctcact ataccggaat gagaggatta gaggaactgc
     8161 gggaagcggc ttgtatattt cagcaacagc gttatggatt aacttatgat ccgcaaacgg
     8221 aagttttgac gacagttggt gcaacggaag cgattgcctc tgcgttgtta tctgttttgg
     8281 aagaaggaga caaggttctt attccagcgc ctgcttattc aggatatcag ccattagtgg
     8341 aactagctgg tgctgaattg atcccgatag atacttctga cacagggttt gtctgtcagc
     8401 cggaacaatt tgaacgagca tttgaacaat atggatcagc tgtcaaagca gtcattttaa
     8461 actatcctaa caatcctact gggacgacat tgtctgcaaa acaattgaaa gcaatcgctg
     8521 aggttttaaa aaaatatccg gtatttgtta taagtgatga agtatatgct gaattgactt
     8581 atagtggaac gcatatgtcc atagctactt atcttccaga acaaacaatc gtgataagcg
     8641 ggttatccaa gtcacatgcg atgactggtt ggcgagtggg ctttattttt gctcaaaaag
     8701 cccttattga cgaactaatt aaagtccatc aatatctggt gactgccgct acaacgatgt
     8761 cgcaaaaagc tgcggctgaa gcgctaataa atggggtgaa agatagcgag aatatgaaag
     8821 agcaatatca aacacgaaga gactatctgg tccagcaatt ggcgccttta ggatttgaag
     8881 tgacccagcc aaacggtgca ttttacttgt tttgcaaact cccagctgcc attcaaactg
     8941 actcttggca gttttgtgta gaattagcgg aacaggcaaa agttgcgtgt atcccaggaa
     9001 ttgcatttgg cccagaagga gaaggttata tccggatttc ttatgctagc agcatggaaa
     9061 aattacatga ggcctgcaaa cggatcaaag actttttaac agaacagtga ttcatagatt
     9121 ttatcaaaaa aacaatcgtt aattttgcgg tatattctgc aaggagacga ttgtttttta
     9181 aatttagttc aaatgaacgc gtttcttttg attcataact aatgtaatgt taagtttaaa
     9241 ttgtttagaa tgattttaaa agaatattca aggaggtttc atcaatggaa aaaatcaaag
     9301 ttgtcattgt aggtgcatct catggagggc atcaatcgat cttggaacta ttgacacgat
     9361 acggtgaaaa tgtagatatc acgctatttg aagcaggaga ctatatttcc ttcatgtcat
     9421 gtggtatgga gctgtattta gaagacaagg taacagatgt aaatgatgta cgtaatttca
     9481 gaccagaaaa ctttccgcaa tccaatgttc atattttaaa taatcacgaa gtaaaaacga
     9541 tcgatgccga taaaaaaaca gtaacggtta ctagcgcatc agataacgaa acaaaagaat
     9601 atccttatga caaattgatt ttgagttctg gtgtcaaacc aaacaatctg ccagtaccag
     9661 gagcagatct agaaaatgtc tacttgatgc gtggatacaa ttgggcaacc aaaatcaaag
     9721 aaaaattaaa tgatccggct attaaaaaaa tcgctgtcgt aggtgcgggt tacattggga
     9781 tcgaagcagc agaagcaagc cgaaaagctg ggaaagaagt cgtattgctt gatgtgatcg
     9841 atagaccttt aggcacttat ttagatgctg agatgacaga tattttagaa caacacctaa
     9901 atgaaaacgg tattgaagtt ttgacaaatg caaaaatcga agcttttact ggtaacggaa
     9961 aagttgaggc aatcaaaaca agtgaaaaag aaatcccagc agatctagtc attcaagctg
    10021 ccggtgtgaa ggcaaataca gaatggttaa aagggatcgt cgacttagat gaacgtggct
    10081 ggatccaaac aaatgaatac ttgcaaacga atcttccaga tgtgtatgca gtaggagatg
    10141 ctacacttgc ttattctatc ccagcacgca aaaaaatgcc gatcgcttta gcgactgttg
    10201 cacgtagaga agcacgttat gtagtaaaac atctttttga agaagtacca aacaaaccgt
    10261 tcggcggagt cgtaggttcc tctgcattaa gtgtctttga ctatcatttt gcagcaagcg
    10321 gattgaatag ctttacagca gcaaaagcag gagtagatgt acgagtgtct tattatgaag
    10381 atacgttacg accaaaatat gtaccagaag aacaaggaaa tccaaaagtg tccatccaat
    10441 tattctcgga tccaatgacc catcaattgc taggaggagc agtattatct acctatgatg
    10501 ttacaggtca aggaaatgta ttggcactag cgatccaaaa caaactgact ttagaagaac
    10561 tagcagaagc tgacttcttc ttccagccag gctttgaccg acaatggagc atgttgaatc
    10621 tagccgctca acaagcgtta ggagaagaac catttgtaga ataagaaaac gaaatatttg
    10681 aaaagaggtt gtgaaaaagc tgccttgcat caagtaataa gaacagccaa gtaaaaacag
    10741 cttcttatgt ttttaacttg gctggactta ttaccgcaga tgcaagcttt tgaacaccgt
    10801 ttattttcaa aaaagggact gtgacacgat ttgtgtaccg acccccaaaa gttagaccaa
    10861 aaaatctaac ttttggag
//
LOCUS       DAKBVG010000086        10784 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000086 DAKBVG010000000
VERSION     DAKBVG010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10784
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.086"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(108..1688)
                     /locus_tag="OUD09_001551"
     CDS             complement(108..1688)
                     /locus_tag="OUD09_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCT9692825.1"
                     /translation="MNNPELKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2089..3465)
                     /locus_tag="OUD09_001552"
     CDS             complement(2089..3465)
                     /locus_tag="OUD09_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCT9692826.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(3593..4711)
                     /locus_tag="OUD09_001553"
     CDS             complement(3593..4711)
                     /locus_tag="OUD09_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCT9692827.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            4853..5133
                     /locus_tag="OUD09_001554"
                     /pseudo
     CDS             4853..5133
                     /locus_tag="OUD09_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(5211..5744)
                     /locus_tag="OUD09_001555"
     CDS             complement(5211..5744)
                     /locus_tag="OUD09_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HCT9692828.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(5838..7016)
                     /gene="mutY"
                     /locus_tag="OUD09_001556"
     CDS             complement(5838..7016)
                     /gene="mutY"
                     /locus_tag="OUD09_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCT9692829.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7009..7806)
                     /gene="recX"
                     /locus_tag="OUD09_001557"
     CDS             complement(7009..7806)
                     /gene="recX"
                     /locus_tag="OUD09_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCT9692830.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNRGKSQKELLRKIQQTLMTKGFSQEVIQQAMADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            7913..9295
                     /gene="rlmD"
                     /locus_tag="OUD09_001558"
     CDS             7913..9295
                     /gene="rlmD"
                     /locus_tag="OUD09_001558"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCT9692831.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMICEINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(9469..10047)
                     /locus_tag="OUD09_001559"
     CDS             complement(9469..10047)
                     /locus_tag="OUD09_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HCT9692832.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            10369..>10784
                     /locus_tag="OUD09_001560"
     CDS             10369..>10784
                     /locus_tag="OUD09_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HCT9692833.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGEYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPY"
BASE COUNT         3195 a         2185 c         1856 g         3548 t
ORIGIN      
        1 aagagccgtc acggaaaatc cgtcacggct ctaaacaaaa tatgagaggc tgtttttgtt
       61 tggctattct tattacttga tgcagggcag cttgtgtcaa gacttcttta catcaaactt
      121 tttaatttta cattaggata tttatccgca aaccagcgtt ctgcaaattg gttctcgaat
      181 aagaacaatg gttggtcgaa acggtcacga gccaagatat tgcgacttga actcatcttc
      241 tcatccaaat cttctggatc gatccagcga gcgattttgt gtcccattgg agacatgatt
      301 acttcggcgt tatactcatt taacatacga tattggaata cttcgaattg tagctgacca
      361 acagctccaa tgatatattc ttccgttaga tatgttttat aaagctgaat cgctccttct
      421 tgaactaatt gatagattcc tttgtggaag gatttttgtt tcatgacatt ttttgcagtc
      481 actttcataa acagctctgg agtaaatgaa gggagttctt cataagcaac ttttagtttt
      541 ccttcgaaca atgtatcccc aatctgataa ttgcctgtat catatacacc gatgatatcg
      601 cctgctactg ctttttcgac gttttctctc gaatctgcca taaattgtgt gacattgctt
      661 aatttcagtt ttttatttgt acgttccaac cagacatcca ttcctcgttc gaatgttcca
      721 gaacagattc ggacaaaagc gattcggtca cgatgggctg gattcatatt tgcttggatc
      781 ttgaatacga aacctgaaaa ttcttcctca taaggactta ccatctgatc atcttgtgtt
      841 ttgtgtccat aaggagctgg tgccaattca acgaaagtct ctaaaaaagt ctgtacaccg
      901 aagttagtca atgcagaacc aaaaaatact ggtgtctgat cgccacgagc aattttttct
      961 tgattaaatg catctccagc ctctttgatt agttctactt cttctaagac ttgttcatat
     1021 aaggaatttt cagttagagg atgattttcg ggaagcagtc catcttcatc caattctact
     1081 aatcgttcat cctgataatt ttccggacga taaaactcta cacgattatg gtagatatca
     1141 taaagacctt ctagcccttt acccataccg attggccaat tcatcggata agattcgata
     1201 tctaatagct cttctaattc ttctaacaat tccaatggtt cacgaccatc acgatctagt
     1261 ttgttgataa aagtaaatat cgggatgcca cgttttttta caacttggaa taatttcttt
     1321 gtctgtgctt cgatcccttt tgcactatcg attaccatga ccgcactatc cacggccatc
     1381 aaagtacgat aagtatcttc agaaaaatct tcatgtcctg gtgtatccaa gatatttact
     1441 cgtttccctt gataatcaaa ctgcataaca gaactagtaa cagaaatccc acgttgtttc
     1501 tcgatttcca tccagtctga tttggcaaaa ttacctgttt ttttgccttt tacggtcccg
     1561 gcttgacgga tagccccacc aaacaatagt aattgttctg taatcgttgt tttcccagca
     1621 tccggatggg aaatgatcgc aaaagttctg cgattgtcca cttgttgctt taattctgga
     1681 ttattcataa cctaactcca tttcttccat tcaattattt atattttctc taggcacaga
     1741 ttgtccgatc tcttaaagaa agacaatttc atatagggct gtgacaaaag ccgtgtagca
     1801 gtctcttttt ccgagtaaac ggcgttctag agacagccta tcagcaataa gcccacccaa
     1861 gccaaaaata cgaaaagctg tttttgcttg gctgctctta ttacttaatg caggatagct
     1921 ttttcacaac ctttgtcaac tactacaact gaatcagtat agcatggatt catcgaatga
     1981 aaaagccacc aaaccataag agtcaaatat ttttttgtca gctgactaaa agaaaagacg
     2041 tgttgcttgt ttattcaagc aacacgtctt tcataaccaa gtttccaatt atcttcttgg
     2101 ctcgtcatct tcgaattctt ttctgaagta tcttcgttct tcttcaggtt ccgcttctgt
     2161 ttcttccgga tcatgaaaaa tgacacggat tttaaggatt cgagaacctt ccatttcttc
     2221 agaaaccagc gtaatattat cgacatcaaa cgataacttt tcgccttcat ccggaatcat
     2281 gcctaatgct gtaatcaaat aaccagccat cgtatctaca tcactcatat gcagatcact
     2341 gtcaaaagct tcattgaact catcaatcag catacgtcct tggatgatat attcgtgttc
     2401 atcgattttt tcataaagat tctcaacttc atctgattca tcatcgattt ctccgacgat
     2461 ttcttctaaa aggtcttcta gtgttgctaa cccaacgacg ccgccatatt catctaataa
     2521 aatagccatc tgattctgcg tctttttcat ttcatataac agatcatcaa taaagaccgt
     2581 ttcaggaacg aataaaggct cctgcatgat tttcttgata tctaaattat caaaaccgaa
     2641 tttatgggca gcttttagta aattctttgt atgaaggatt ccgactactt tatctttgtc
     2701 ttcgttataa acagggattc ttgaatagtt ttcggataaa acttcattca cattttcttc
     2761 cacagtgtcg ttgatatcaa tcataaatgc atccgttcgt gggaccatga cttctcgggc
     2821 aactttcgta tcaagagaaa aaaccccttg gagcatttct aattcttcat tattcaatac
     2881 gccttcgttt tcaagcatat aacgcatttc atctctggtc atttttgaat cttcatcatc
     2941 aaaagtcatc ggcgtgatac gcgccaataa acttgtggaa gcagaaagca gccagacgaa
     3001 gggcttagct actacaccaa tcaagcgtat cagccctgaa gtgaactttg cgacttcttc
     3061 tgatttattt aaagcaatac gtttagggta caattctcca aaaacaatcg atacataggt
     3121 caaaatagcc agcacaatga tatttgcaat attttttgcc gctgtgccgc cacctaatac
     3181 cggagctaac cgttccgata aagtattagc tagtgaagca ccagacaaaa tgttgaccaa
     3241 agtgattcct acttggattg tcgataggaa attatttgga ttctgcaata tttttaaaag
     3301 cttttgagat ttcacatctc cttcatcagc tttttgctcg attcgattct tattgatcga
     3361 aacaacagca atttcggaag ctgctaaaaa agcattgatc aatgtcaaaa cgatcaataa
     3421 taaaatctgc gctaacagcg actgactctc agggtcagca ttcataatcg cattctctcc
     3481 ttataaaatt aatttttagt ttacgataga aaaaaacgtg gcactatagc cacgttaaaa
     3541 tggtatcaca aagcaatttt ctttgcaata aaaacaagtg tactaaaact acctacgagg
     3601 tcgtagcaat tttcttcttg ttttcatttt gcttgtcttc tttgacgatt ttgattatat
     3661 aggaaatgat cgtcttgcag attgcgtaaa aaggaacacc taagaagata ccaagcaatc
     3721 cggcaatatt tccagctact agaagaacga taatgatcgt taatggatgg attttcagtg
     3781 atttcccaat tacattagga taaatgatat tcccatccaa ctgttggacg attagcacaa
     3841 ccacacaaca aagtaatgct tttaccggat cattgaagac tgttactaga aatgcaggtg
     3901 ctagtcctag ataaggtcct aaatatggaa ttaggtttgt caatcccgca atgaccccaa
     3961 ataagaaagc ataatccaca ccaattacca tatatcctaa aaatgtgaag gtaccaacga
     4021 ataaacattc gattgcttgt ccgctaatgt aattagccaa tgttttattc agttgcccca
     4081 ataactccac aatttggttt cttctttttt ctgggaaaaa ctgttggatg tttggcacta
     4141 gtttttctcc gtctttcaac atatagaaca agatgaatgg tgctgtaatg attactacgg
     4201 ttgccgaagc gatcgttcca acgatcgaac ctaaactatt ggatactcca ctaaggaatt
     4261 gctgaatgat tgtcccgtaa gaaatgtcta attgctcaaa atatttattg atatctactt
     4321 ccttgaacaa aggcaattcg gctacctctt ttaaccagct ttctacctgt ttgatgaaat
     4381 caggcatatt agatgccaat gaggaaattt gctgtactag acttgggatg acactaagta
     4441 aaatccaaat aattgctgca actaaaagga gcagcacaat tgcgacagcc catattcttt
     4501 tgatttttgc tttcatcaac agcttgacaa taggattcaa taaatagtaa agaaagcctg
     4561 caatcagtac aggtgcaaat aacgtggaaa aaaatgtccc gatcggttcg aaaataaagt
     4621 ttatttctga tgaaatcatg atcaatgcag ctatgatcaa taattcaagc gaccaaaaca
     4681 ttagtttaga ctgtctaagt ttttcaaaca atagatttcc tcctttcttt cttactagta
     4741 tatattatat tcgttaaaaa ttcttttatt ctttttttat ttttgaaaac ttgccatact
     4801 ttttatttgt tttattttcc agtatactat acgaaaggaa ggtggctaga ccatgaaagt
     4861 tgtacaaaca aaagatacaa tgagcaatat ttacttagat gctgtacgga tcaggcatca
     4921 agtttttgta gtcgaacagg gtatgccttt gtctcgagaa atagacaaag atgaagccca
     4981 ttgtatccac ttcgtcctgt actcagataa aaaagaaccg caaggaactg tacgtctttt
     5041 gcctttagaa aacggaaaaa tgaaattaca gcggatggcg atccttttga agatgctggc
     5101 atgcctcata tttatatgaa aaagacttta taaaaattta tttcgaaaaa agcaaaaaac
     5161 agcaagcttt tgagaaaaat ctctcactgg cttactgttc ttttttgtgt ttacttacgc
     5221 gacaattgct tgtaacggtt ataccaaata tcaatatagc cttccgagaa cgggccatct
     5281 ccattattga tccagtcaac cagaatcttc acattttctt ttaggatgaa gtcaatatcg
     5341 ttcggataat tcatcatttt gctgtgcatc tcatattcat cgacatccaa tagtcgtttc
     5401 tcgccgtcag gaaatacctt gatatccaag tcatagtcga tatatttcaa ggcctcatcg
     5461 tccagcaaaa aaggtgaagc tagattacaa taataggaaa cccccttttc tctgatcatt
     5521 gctattacat tgaaccagta ttttttatga aaataaacaa tcgctggttc gcgcgttacc
     5581 catcggcgtc catcagattc agtgactaat gtgtgatcat tcacgccgat catagagtgt
     5641 tcgcttgttt ttaataccat ggtatctcgc cacgttcgat gcaatttgcc gtcgtgcttg
     5701 taactttgga tcgtcacaaa ctctccttct tttggaactc ccatagcaac ccttccttaa
     5761 gcagcatttt gtgctttgtg tttgggtgtt cattacaccg acatcataat tattatatca
     5821 ataaaaatga gctttgccta gaaaggattc ctattgtttc gatttttctc taactgatct
     5881 accagtttca tttggacctt aggaaaaacg accgaatcaa atgcatcagg cttcaaccac
     5941 ttcgaagttt tgttctcggt aaattcttgt tctgctccct cggttgcccg tccgtaaaaa
     6001 aggagaacat gccatttcag atgactaaaa atatgagtaa cttctcctaa atgtcgtgtc
     6061 tgccaaacaa agagttcctg cttttcaaat gggagatttt gctcttcttc tgctactagg
     6121 tcatcaaaca gatcgatttc tggctttgtt tcccattctt ttttcaatcg ctcatactcc
     6181 tcttgtgtca cttccatcat tggaaatgtc cacatattcg ccagcaattt ttggctatct
     6241 ctttcttcaa aataatacgc tccagaatga ttttgaagag ccgcactgat ataataaacg
     6301 tcttttggct ttgctttttt tgtttttaca ggaaaactcg tttggattcc tcgtttgtta
     6361 gctaaacaaa aggcttggat cggacaagtc tcacactttg gagaagttgg tgtacagata
     6421 gctgaaccta gatccatcat tgcttggttg aattcacctg gatgcttttc atcaatgatt
     6481 ttccgcatcg cttcatcaaa gattttcctg ctagaagctt ttgcgatgtc tgcttcaatg
     6541 caaaacaatc tgctgactac tcgcatcacg ttgccgtcaa ccgcgggttc tggaagtcca
     6601 aatgcgatac ttgcaatcgc gcctgtcgta tatggtccta ttcccttcaa tgagctaatt
     6661 tcttcaggcg tttgcggcat ttccccatca aattctgaca tgatctgttt agcagcagct
     6721 tggatgttcc gggctcttga gtaataacca aggccctccc atgcttttaa aagcttttct
     6781 tctggagcat ttgctagctc ttcaattgta ggaaaccatt ccatgaaacg ataaaaataa
     6841 tcgatcactg tgtctactct ggtttgctga agcatgatct cagagatcca gatacgataa
     6901 ggatcacgat tatagcgcca aggcaggttt cttttttctt gttcatacca ttgaatgaac
     6961 tgatcttgaa attcttttgt ttctttatct gtccattttt cccattcttt attcactttc
     7021 ctcttccttt tccgtaatga atcgctggat catatccaaa tcaaatcctt tttgatataa
     7081 gctttgcttt acttttagat ttcttttttt aggttcaaac cttaaatttc ttcgccatag
     7141 tttatctcct tgtttgacta aataatcata ttctatttct tgatctgctt cttgcggaag
     7201 atcagccatc gcttgttgga tcacttcttg agaaaaacct ttcgtcatca atgtttgttg
     7261 gatttttctc aataattctt tttggctttt tccgcgattt tttccaaacg ctttttcggc
     7321 tgtctgatgt gcattttgaa cttggtcttc aaatgtatat tggctgagcg cttgttcaat
     7381 gatttcaggt ttcaaacctt tttgctgcat ctgctgcgct aatttctttg gtcctttgtc
     7441 acttaagcgc atattcgtgc gcacataact ttccccatag accaaatcat cgacaaggtc
     7501 caattctttt aagcgggcta cgattttatt tcgatcttct tgaaggattt ccttttcttt
     7561 taagtatgtt cggatttctt tttccgatcg caactgataa cttaagtaat tcattgctag
     7621 gtgaaaaccg aaatcagctc cgctactctt cttgatctct tggatagttt cttcgtctaa
     7681 ttcccgtcct tttaataatc gatagcgtac taatacatct tcagatactt gtaaagcttc
     7741 tccttcagat aaatctacgc gatagaaagg gcctttgcct ttactgattc tcgtaacggt
     7801 taacataaac atcctcctcg aacatatatt ctctatcata ccacagaaaa tccccgtaat
     7861 caggtattat atgttagaat aaaccacaga aaaaagaaaa agggagatac ttatggcaga
     7921 aacaattgtc aaaactggac aaacggttcc tttgaaaatc aagcgtttag gcataaatgg
     7981 tgaaggaatc ggttatttca aacggttgat catatttgtt ccatatgcac taccaaaaga
     8041 agaagtacta gtgaaaatca cgaaagcaac tcctcgctat gcagaaggag aattaataaa
     8101 agtcaaaaaa acaagcaaag atcgcgtagt ggcaccatgt cctgtttact atgaatgcgg
     8161 tggctgtcaa ttacaacact tagcttatca agctcaattg gattttaaaa aggatctgct
     8221 tttgcaagca ttggaaaaat tcaaaccagc aggctttcga tcttatcaac ttttgccgac
     8281 gatcgggatg gatgagcctt ggcattatag aaacaaagca caatttcagt tacgaaaaaa
     8341 taagcagact caaaaaattg aagctggttt atacgaagca aactctcatc agttagtccc
     8401 tataacagac tgcttggttc aagaacctcg aacacaaaaa gtaatgaata ctgttgttca
     8461 actattgaat aaatttgatg cacccatcta tgatgaacgg acaaacagtg gaatcttccg
     8521 cacaatcatg gttagaatcg gtgttaaaac tggggagtta caagttgttt ttattactcg
     8581 cagtaaaaaa ttcccgcaaa aaaacgcaat gatctgtgag atcaatgaac gactgcccga
     8641 agttgtttca atcatgcaaa atgtccaggc acaaaaaacg tctctcgtga tgggggatga
     8701 aacgatccac ttatggggaa aagaagccat tgaagaacaa atcaatgaag tgactttcga
     8761 tctttctcct cgtgccttct ttcaactcaa tcctcagcag accgaattgc tttatggcga
     8821 aggaatcaaa gcattagatg tccagcctaa tgaaacgatc gtggatgctt attgcggagt
     8881 tgggacgatc ggcttaagtg tagcaaaaca agccaaagaa gtacggggaa tggataccgt
     8941 tcctcaagca atcgctgatg caaaaaaaaa tgcggaaaga ttaggatatc acaacactcg
     9001 atatgaagtc gggacagcag agcaagttct gcctaaatgg ttgaatgaag gttttcagcc
     9061 tgatggtatc atcgtagatc caccacgtac gggtttggat aacgcattga tccaatcatt
     9121 attgaaatac cctccaaaaa aacttgttta tatctcctgt aatgtttcta cacttgccag
     9181 agatctggtt aatttaagta aaaagttcaa gattgaatat ttacagtctg tcgacatgtt
     9241 tcctcagact gcacgttgtg aagttgtcgt gaagatgacg agactcgctg actaaatgaa
     9301 agaggttgtg aaaaagtctg ccctgtatca agtaataaga acagccaaac aaaaatagct
     9361 cttcatgttt tatgaatacc gtacttgtga ccgaagagtt aacaggagga cactatttat
     9421 ttaaaaaaag ggttgtgaca tagtttttgt cacagccctt tttgattatt attggatatt
     9481 cacgtctatc acttgataaa atgcatttgt cgtatcgctg atattccata ctgctaaaat
     9541 cacattatat ccttttcgat cctgcggaag tgtgatcgtt tggttgactg tttttggtgg
     9601 aacagttgct ttgtcgtcta tttttgtgat caattcaaaa tcactaaatt tcaaaggttg
     9661 attaggattc caagttggtt tggtaatgta gtaatcccaa gtacttgtcc gatgttgtgc
     9721 tgttaaattc caagtaatat ttaatgttcc tggattaacc atgtttttat gccatctgtt
     9781 agatgtctgt tcatctaaag gctcaaagcc gctgactcct gcactagcaa tttttccatc
     9841 aatgaatgta tttttggtag cttcgatact ttgcggttcc cattgtgctc ttccgacatt
     9901 ttgatttaag tttcctgctg ctgatgttcc taggtatgca cggcttcctg gagaagtaat
     9961 ataaccatgc gcaaaacctg gaatgctaaa accgaacaac cctaaactaa ctaacccaat
    10021 aaacaataac gtactaatct ttttcatgta gaaacctcct tcatttgata accacattgt
    10081 aaacgcttta ttgtattaac acaaacatta tgcttttata attttatgtt gaagtaaaaa
    10141 aggtgaaaaa agtaagatgc agattataac agaagtagac gagaaaagta caaatagaaa
    10201 acatgagaac caaatcatat gatctttttc aaaaacatca ttcatataaa caaatgttca
    10261 actttttaat aacaacaatc atcaattttt ttcttacttc acaaactaaa tacaggtctt
    10321 gctgtgagcc ttcatggtaa actactatta tagaaaggag ttagtaaaat gaagatgata
    10381 aaagatattc gtttttgggt ttgtgttata ggaattgtca tattaggctt tttatcaggg
    10441 ttgcttagcg gaaatcccgg agaatattac tattctttgc aacttccacc atttgcacca
    10501 cctagctgga tatttgggcc gatgtggacc ttactgtata tattgatggg tatttctctt
    10561 tatttgttac ttaatcacaa caacaaaaaa cagcgaaaca atcttgtcgg tctttttgtg
    10621 attcagttca tttttaattt tatatggtct gctctatttt ttaatttaag aaatattttc
    10681 attgcagcaa tcgatattac acttttagtt attttcttgt cagtgcttat gtatcagttg
    10741 tggctacatc atcgcctggc aatgtggctc atgattcctt atta
//
LOCUS       DAKBVG010000087        10679 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000087 DAKBVG010000000
VERSION     DAKBVG010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10679
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.087"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(<1..1251)
                     /locus_tag="OUD09_001561"
     CDS             complement(<1..1251)
                     /locus_tag="OUD09_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT9692834.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNER"
     gene            complement(1244..2143)
                     /locus_tag="OUD09_001562"
     CDS             complement(1244..2143)
                     /locus_tag="OUD09_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT9692835.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            complement(2288..4066)
                     /gene="uvrC"
                     /locus_tag="OUD09_001563"
     CDS             complement(2288..4066)
                     /gene="uvrC"
                     /locus_tag="OUD09_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCT9692836.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            complement(4248..4562)
                     /gene="trxA"
                     /locus_tag="OUD09_001564"
     CDS             complement(4248..4562)
                     /gene="trxA"
                     /locus_tag="OUD09_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HCT9692837.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            complement(4649..7009)
                     /locus_tag="OUD09_001565"
     CDS             complement(4649..7009)
                     /locus_tag="OUD09_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HCT9692838.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            complement(7124..7672)
                     /locus_tag="OUD09_001566"
     CDS             complement(7124..7672)
                     /locus_tag="OUD09_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HCT9692839.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            complement(7730..8167)
                     /gene="zapA"
                     /locus_tag="OUD09_001567"
     CDS             complement(7730..8167)
                     /gene="zapA"
                     /locus_tag="OUD09_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCT9692840.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            8369..9286
                     /locus_tag="OUD09_001568"
     CDS             8369..9286
                     /locus_tag="OUD09_001568"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HCT9692841.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            complement(9542..10453)
                     /locus_tag="OUD09_001569"
     CDS             complement(9542..10453)
                     /locus_tag="OUD09_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCT9692842.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
BASE COUNT         3007 a         2291 c         1798 g         3583 t
ORIGIN      
        1 tcgttcattt ctcataattg aaatcgattg tttcaatgaa ctccataatc cgccttcgct
       61 atacacgagt acattcgatt tgtacatgtt tgcagacaag atcatcaata agaatgttac
      121 ggctacaagt accaacaagg aaatcaatgc tccacccata ccgactgtct catttgctaa
      181 gcgaaccggc ataatatatg aagataagaa tggaatatac gaagtgatcc gaataacgat
      241 attgtttgga tctgaagctc ctagtatcaa tccaagcata tatccgccta gagatagata
      301 agtcactggc aaaattgctt ttgctgtatc ttctgctttg ttgaccaaag agccacataa
      361 agctgctaaa acagaaaaaa tcaaaatgcc aaagatcatg aatagaagag aataccacaa
      421 gaatggtcca atcaagttat ccaatgagat accgtctaat atatttttga ccatttccat
      481 atctttgaat tgactgtatc ctataccaaa aataataccg tataatgcca tttgcgttaa
      541 tgcaacaagt aaaacacctg ttagttttcc gtaaaaatgc ttttgtgcag tcgtactgga
      601 caggatcact tccatgatcc gcgtgccttt ttcagaagcg atctcttgtg caatgatctg
      661 cgcatacgtc aaaataataa tgaacaagac aatcgttgct acataactta cgacatattg
      721 gactgtcgag ttatcttctc caactgtcat ttttcctttt gaatcaaaat tgactttttg
      781 tcgagtcaaa cttgcttgtt ggctcaagct agctacttct tctggtgaaa tgcctaattg
      841 acttgctcga agcatcgatt gcaagccagt cagttgttgt tgtatcagta gttgtgtact
      901 ttgtcctaat gaattttcgc tataaagcat cccatttatc ttatcattct cagttgagac
      961 tactaaatac gcatcgattt tttcttccga aagctctttt tgtgcttttt cttcagaatc
     1021 aactgctttg aactcaaaat catctgtttt gacttggctc atttgctgac tgatttgctc
     1081 attatcagcc aatacgccga ttttattgac atcactattt tgttgagcaa agtttcctgc
     1141 tacataaatg atacccagca tgataaaagg gactaatatc atgatcaaga aagaaatcga
     1201 cttaacattt tttaaataga catctttggt aataatccaa aacttacgca tcgatttcac
     1261 ctgctttcaa tttgaaaatc tcttccaaag ttggcggttg ctggttaaac ataggaatgt
     1321 agccgaactg agtcgcacga gtaaagattt ctttcccagc ttcaggatct tctaaggtaa
     1381 tctctaacgt attgtcttca tgaaggatca cttttttcac gccttcgatt tcttctactt
     1441 cctgttggct taaaccagat tccaagaata acttcgtacg gccaaatgat tcacgaatct
     1501 catgtacttt tccattcaaa accatttttc cgtttcgcaa catgatcaaa tgatcgcaga
     1561 ttttttctac attatccata ttgtggctag aaaaaatgac acatgaacca tgttttttca
     1621 attccaatat tccatctttt agcaattcag catttaccgg atctaacccg ctgaatggct
     1681 catccaaaat gatcaattta ggttcatgga ttaacgtggc gatcagctgg actttttgct
     1741 gatttccttt agacaaagat tttactttat ccgtcttttt cccttttacc tgaaattttt
     1801 ccatccaaaa atcgattttt ggttcaatct cttttcttga tttccctcta agttctgcaa
     1861 aataaagcag ttggtcttga atcgttactt tcggataaag tccccgttcc tccggaagat
     1921 aaccgatgac atcataatct ttctccccaa gatgatggcc attccataac acttctcctt
     1981 ggtcttgtgt caaaaaatcc aagatcaaac gaaaagtcgt tgtcttccca gccccgtttt
     2041 ggccaattag ccccatgatt tttccatcgg ggatttggaa agagacatga tcgacagctg
     2101 tgtagttccc aaaagtttta acaagatcct tgacttctaa catttcttca ctccttcttc
     2161 aagaaatcac atatatcctt agacaccaga cgtaagcgat ttattattta ccaacaaaac
     2221 gtaaacgctc tttttgttgt acataaaatg ataggacact ttactttctt tgtaaagtgc
     2281 cctttcctca ttcctgatgt aatttatcaa atacgttttg tgcgacattt ttcggcagtc
     2341 cgatttcttg taattcttct gtcgtggctg ctgtgatatt tttcaacgat ttgaactctt
     2401 taagcagcat tttttttcgt tttggtccta acccttcaat ttcatctaat cgggaagcaa
     2461 aactgttttt actgcgtagc tgccgatgga aagtgattgc gaatcgatgg acttcatctt
     2521 ggattcgctg gagtaagaaa aactctggtg aatttcgctc aagcggtact acagccaaat
     2581 ctataccgaa tagcaactca tttgttttat gtttatcgtt ttttgccatg ccagcaatag
     2641 gaatatcgat tcctaattga ttttctaaga catcttttgc cacatctact tgtccttttc
     2701 ctccatcgat caagatcaaa tcaggtaaag gcaacccttc gcgaatcact cgtgaatatc
     2761 tgcgatagat gacttctctc attgatgcat aatcatccgg tccgttgact gtttttattt
     2821 tatatttccg atattctttt cttgccggtt ttccatcaat gaatacgacc atcgcagcca
     2881 caggattcgt ccccatgata ttagagttat caaaagactc gatacgtatt ggggtcggga
     2941 tattcatcgc gtttcctaaa cgttcaactg ctccgatcgt ccgttcctgt tttctggcaa
     3001 tcagatcaaa ccgttcattc aaagcaactg aggcattctt attcgccaat tcaactaatt
     3061 ttttcttttc tcctcgtttt ggttgcagca ctttcgtagc taacaaagct tctacactgg
     3121 gcttgtcgat cgtatcagga atcaagactt ctttaggaat gaaatgctca ttttcctgat
     3181 aaaactgacc gaggtaagtc aagaaatctt cttctgcttc gttatagaaa ggaaacattg
     3241 aaacatctcg ttcaattagc tttccttgtc ttacgaaaaa cacttggaca cacatccatc
     3301 ctttgtcaat cgcataacca aatacatctc gatccagtaa atcagtattt gtcatttttt
     3361 ggcgagtcat gattgtctca atggcacgga tctggtctcg gtattctgca gctttttcaa
     3421 actccatgtt ttcagcagct ttttccattt tctgatggat ctctgactcg attttttcgt
     3481 gtcctccgtt taaaaacttt ttcacttcat caacgatttt cgtatagact ttcggatcta
     3541 cgtcgaaata ataaggacac agacattgtc ccaaatgata gtaaagacaa ggctccttcg
     3601 tctgactagg tttgcatttt ctcagaggaa ataatcgatc gaggatcttc tttgtctcat
     3661 tcgccgcacc gacatcggga tatggtccaa aatacagtgc tttgtctttt agtactttac
     3721 gagtaatcac caaacgggga tatttttcat tcgttatctt aaggaaagga taggttttat
     3781 catcctttaa cataatgttg tatttaggat catttttctt tatcagattg atctctaaga
     3841 gcaatgcttc gatattcgat tctgtaacga tgtattcaaa gtcttcgatt tcactaacaa
     3901 gccgttccgt tttcgtatta tggctgccag taaaatatga gcggacacga ttttttaata
     3961 ttttggcttt gccgacataa atgatcgttc catttttatc tttcatcaag taacagccgg
     4021 gctgatcagg aagtaaagct aatttgtttt tgattcgttc gttcatctgg tactcccctt
     4081 catattcaat taaagttatt atagcatgtc tgtcaaaaaa acaatccagc ctgacaacgt
     4141 aattatactc aaaaaatatt aatttgaata accttaactc aaccgcaaaa aaaccgagga
     4201 cggaactgga ttcagtccca ccctcagctt atttttgctg taagttttta taaatattga
     4261 tcgatcattt gacgtagttg ttcttttgaa tgaacaccaa cagctttttc tactacttga
     4321 ccatcttttt tcaacattaa tgttggaatg ctcatgatac cgaattgttg aggagtttct
     4381 ggattttcat ccacatccat tttagcaatt ctgaattctt cttcatcata ttcttgttcc
     4441 aactgatcca aaatcggtgc ttgcatacga catggaccac accaagttgc ccaaaaatca
     4501 atcaatacaa gtcctttatc tgtttcttca ttaaatgtct tatctgtaat tacttgtgac
     4561 atatcaaaga cctccatttt taaaatctat gattagtata gcatttctat acttggatag
     4621 cgaaccatct gcttaccttt agtaagcttt acttaaattt aacaattgtc gcaccatttc
     4681 cgccttggtt tgccggtgca aactcaaagc ttttgacgct ccgatggttt ttaagataat
     4741 cagtaattcc cttgcgaagt gcgcctgttc cttttccgtg gacaatagtc acttgaggat
     4801 aaccagctag aatggcagaa tccaaatatt gatctacctc acttagcgct tcttcgtatc
     4861 gttttcctcg aaggtccaat tgattcggca cgtgactact agattcagat cgtacagcat
     4921 ggataactcg ttgtgtcggc tctttttgag gagcaacagg cgtcagctcg tcttcggaca
     4981 cattcatttt taatataccg atttctactt gccactgatt cccgttttta cgtaaaagcg
     5041 tccctctttg gccgtaagtc gtaaccaaca cttcgtctcc cggtttcaac gttttttgtt
     5101 ctttcgcctt cttcaatact ttatttttct tcaaagtttc ttcttgatgt agatttgcta
     5161 attgggtctt tgcatcgatc aactgatgct ctttcacatt tccttgccca atctgctgtt
     5221 gcattttacg aatatcagca atgatttttt ctgctttttc ttccgcctct gacacgactt
     5281 cgtttgcttt tttctttgct ttttccattt ctttttcacg ttcttcaaag aaatagctgt
     5341 aagcttcttt caaatcatcg tgaagttctt gtgcctcaga aacaaaatga cgcatttcca
     5401 gatattcggt ctctgccatt ttccggcgat tctctaaatc tgtgatcatt tcgtttaaat
     5461 cttggctctc atcattcatc aactgcttcg cttcatcaat aacttctgta tcaagaccta
     5521 atcggctaga gatttcaaac gcattgcttc gtccaggaac gccaatcagc aaacgatacg
     5581 ttggacttag tgtatccaca tcgaactcca tgctggcatt aatcgtattt gcccgattgt
     5641 atccataaac tttcaattca ggataatgcg ttgttgccat cacataagca gactttttac
     5701 caagatcatc taagattgcg attgctagag cagcaccttc ttgcggatct gttcctgcac
     5761 cgagctcgtc aaataatacc agactttttt cattgacttt tgataaaata tctacagtgt
     5821 tcgtcatatg agaagaaaag gtcgatagac tttgctcaat cgactgttcg tctcctatat
     5881 ccgcaaatac ttcctcaaaa ataccgattt gactttcttc acctgcagga atcggtaatc
     5941 ctgcttgccc catcagttgt aacagaccta aagtcttcaa cgtgatcgtt ttcccacctg
     6001 tgttaggtcc tgtaatgacg atggcttgat agtcttttcc gattgtgatg tcattagaaa
     6061 ctactttttc ctgatcgatc agtggatgtc ttgcttgttt caagacgaca tgattgtctt
     6121 cactgattcc cggcacgatg gcttttactt ctttaccaaa tcgtgctttt gcattcatca
     6181 gatccatttt accaatcaca taagcattgt ggatgatttc ttgacgatgg ggaacaagtt
     6241 cagcagaaag ttcacttaaa atacgttgaa tctcatttcg ttcagcgatt tggtattgac
     6301 gcaaacgatt attcaaatcc accacttgtt ttggttcaat aaataaagtc tgtcctgaag
     6361 cactttgatc gtggactaca ccgccaaata caccgcggta ttcttgtttg actgggataa
     6421 cgtagcggtc atttcgcatg gtgatgatcg catcacttaa atactttgca tttttcccac
     6481 ggacgatccc atctaactgt tcacgaacag cttgttcact tcgccgaatg ttctgtctta
     6541 tgctctttaa ctcaggagaa gcgtcatcag tcacgcgtcc gtcttcatcg attgcttcct
     6601 ttaaacggcg gctcaaaaca gggatcgtga ctaactgatc tgcccacata tacaaacgtg
     6661 caaattcgat ttcactgtca gaaagatcat caatgaaacg attcaattcg cttgtcgtag
     6721 acaatactct ggaaacttgt gcaagctcca ctccattcaa gtctgctccg atctcgatcc
     6781 gtttcatatg gggacggata ttttcaagtt taggtatagg gatcccgcct cgcagacgtt
     6841 gcactttcaa tccatcctca gtttcttgaa gccagtttgt gatcgtttgt ttctcactca
     6901 ctggtaccaa ttctgccagt tcttcttttc cttgtgcagt cactacaaat tgtctgacca
     6961 tctgtttgac tttttcaaat tccaatgttt caaggattcg gttattcatt ttatcacctt
     7021 atctttcact atgaaaaaag agctgtgccg gacatacgct acgcagcctt cttaaataaa
     7081 ctactctgta caaaaaacga aatattttcc agcactattt tccttaattc aaaatattcg
     7141 ttacccaaag ctcatacatc ttgttggtaa gaaatggcgt tctctcaacg ataaaacgag
     7201 cgaaactatg ctcgccgaat tgattttgga ccacatctaa tggaacaaag gcaagtaact
     7261 gcaaaatcaa aaatatagta acgtaagcca tgattagtga caggatgcct cctgcaatcc
     7321 agtctacctg tctcaagatc ggcacatacg tcaatccatg gacaaaaacc cccacaaatc
     7381 gggtaagcag ccagccaatt agcaaaataa tcagaaaagc agttcctgca taaaaagctt
     7441 catctaggtg aaaagcaaat acctgatcaa aaaatgctaa ctttgatgtt ggggtgacag
     7501 ctgggtatgg aatgtataat tccaaatgac ttgctaatgg tttgtaaaaa tgctgggcag
     7561 caataaatga caatacatat ccaatcgcga atatgatttg caaagcaaac ccgcgacgtg
     7621 ctccagaaaa aaacgcaatt agcaataaaa ataaaatcag caaactcagc atgctacgtt
     7681 cctttctctt ctaacttttt taaatttttc ttattttgat aaaaggatct taattagaag
     7741 atttttgttt aatttgtcct tttcgtttct cgtttaaaat ctgttgtgct tccacatgat
     7801 ttttaatggt atgatctgct tgaccatttt ctttcaatac ttcccgagct tcgttctcga
     7861 ttgcttcaat gcgtttcaca cgattttcaa gctctgtaaa tttgatcatt tttttcttta
     7921 attctgctgt ttcttcttcc agctccaaga ttctttcttg tttcttcaat tgatccgaaa
     7981 gcgcattgac agccatcaag attgctgctt gttcattatc catttgtggt gataattgtt
     8041 tcaattctgc taactgttca ttgatcagct tcgttacgat atccatatga tgcttggttt
     8101 cccgtccgat aatagtatat gtttggttgg caatcactgc tttgtagcgt gttttttcgt
     8161 gcgccatacg aatcatttcc tcctatttga tgaaagtttt atttagtgta ccatgaagtt
     8221 ggttctttca ccgttctcca caaatatttt tcacatactt aactattata tgataaactt
     8281 taaaaaaatg ctatgtctcg caatctctta gaaaacgaaa agtcgtgagt tgcggtatag
     8341 ttttatgtat aaaaacgagg agaattttat gagcaacgtc attatcaaag caacaaacga
     8401 ccaacttagt caaatgaaag catactatcg ctcttatttg ttaaataaac agattcctta
     8461 tactagcttt gctgcaaaaa aagacgggac gaccattacc gcttatacat ccggaaaagt
     8521 gatgtttcaa ggtacgaatg cagcaataga agctgataaa tggggggagg cacaggataa
     8581 acaagccaag ccagcaaaaa cagcctcttc tttgccaaaa aatatcagtc aattatccgt
     8641 tttaggtagt gatgaggtcg gaaatggcag ctattttggt ccgctaacag tatgtgctgc
     8701 ttatgtgaaa cgtgatcagc ttgcagaatt acgtaaactt ggtgttagag attcaaaaga
     8761 gttaaaagat agccaaatca tccagcttgc tgacatctta aaaaaaacca tcccctataa
     8821 actgcttgtc ttatctccag aaaaatacaa tgagatccaa ccaaaataca atgccgttcg
     8881 gatgaaagtc gctttgcaca atcaagcgat ccatttgctg ctaaaaaaaa tcgagccaga
     8941 aaaaccagaa gcgattttga tcgatcagtt tacgccggaa gcaaatttta aaaaatatgc
     9001 gcgtttagaa aaaaatcaat tacaagaaaa actgtacttc gttactaaag gagaacaata
     9061 tcatttagct gttgctgctg cttctatcat cagtcgtgcc agctttttgg aagagttaca
     9121 caaagcatca gctgaagctg gaatcaccct tccatcagga gctggaacga aatctgacca
     9181 agtagcggca aaactattgg agaaaggcgg catgcctatg cttgaaaaat atgccaaact
     9241 tcattttgcc aatacagaaa aagctttgaa gctgctaaaa aaataatgga actgtgacaa
     9301 aagtcatgtc acagtccctt gttccgaata aacggtgttc tcatgccaac tcctcggtca
     9361 caagtcacaa tagtcatcaa acatgagaag ctgttttttg aaattgctcc ttgtgacgcg
     9421 gagttaaacg gcaggctcac aacctcttcc ttaaacggtg ttcaaaagct tgcatctgcg
     9481 ataataagcc cggccaaacg aaaaatatga ggagctattt ttgtttggct gggcttattt
     9541 attactgaaa ataagtggtc aatgcttgtg tcaaacttgc agcaatctgc tgctgataac
     9601 tgtctgttac taattccttt aaatcactat cattattcat ataacccatc tcgattaaaa
     9661 tagaaggttg aagattttct cgaagaactt gatagttccc agtggagatt cctcttgaat
     9721 ccagaggcag atcagtgaag gagtcagcaa ttgtctgtgc taatgtttcc tcttgtccat
     9781 agtaataata tgtcgtgatt ccagttgctt cattcgcata ttccgtagag tctgcgtgta
     9841 aactgataaa gacatctgcc tttttttctt cactgtagtc gcatatatct ccaagctgta
     9901 ctaattcatc tgtagtatgg gtcaaataaa ccgttgctcc agcatcctct agcgcctttt
     9961 ttacttttat ggctgtactt aatgttatat ctttttcttc tgtttgttca tcattgctta
    10021 atgccccagt atcatttccg ccatgcccag gatctaatac aatcactgca tcagatagct
    10081 cttccggttt tgtttgtata ttctttgtga ctttggttcc atcttcgagg gctaaatatc
    10141 cagtatagcc atcatttgtc ttgatctgat aagcattttt tgtttcagaa agaaactccg
    10201 ccagctctcc gccgtttata gttgcaagtg gggtagttag cgagtcatct gtatatagtg
    10261 tacggtcatt ttttccgatt ttgattaatg tctcttgttc tgtatcatca aatgattcct
    10321 ttgtctttgt aaccgtactg ttcgtactgc tgaccgtttg tccaactatc tttttttgac
    10381 caaaaacatc tatttgaaaa cagataataa tgataatgat tgcaaataca aaagctccaa
    10441 ttattttttt cattcttgac tctcacctcg tagatccaga ctaagggtca aaacttaaaa
    10501 tcagcttaag tacgcaaaaa aggggctgta aaagatataa ccgtagaaaa taacattact
    10561 gctaaaatga tttttttcat ttttggcata tcctgaaatc tccttttgac tattcgtggt
    10621 gattcatcta cccaataaat agtttccttc attctacaaa caattaattt cttcgttat
//
LOCUS       DAKBVG010000088        10637 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000088 DAKBVG010000000
VERSION     DAKBVG010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.088"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(2..559)
                     /gene="frr"
                     /locus_tag="OUD09_001570"
     CDS             complement(2..559)
                     /gene="frr"
                     /locus_tag="OUD09_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCT9692843.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(562..1284)
                     /locus_tag="OUD09_001571"
     CDS             complement(562..1284)
                     /locus_tag="OUD09_001571"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCT9692844.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1420..2301)
                     /locus_tag="OUD09_001572"
     CDS             complement(1420..2301)
                     /locus_tag="OUD09_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCT9692845.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2400..3182)
                     /gene="rpsB"
                     /locus_tag="OUD09_001573"
     CDS             complement(2400..3182)
                     /gene="rpsB"
                     /locus_tag="OUD09_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCT9692846.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3541..4020
                     /locus_tag="OUD09_001574"
     CDS             3541..4020
                     /locus_tag="OUD09_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HCT9692847.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4237..5328
                     /gene="phnW"
                     /locus_tag="OUD09_001575"
     CDS             4237..5328
                     /gene="phnW"
                     /locus_tag="OUD09_001575"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HCT9692848.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5321..5449
                     /locus_tag="OUD09_001576"
     CDS             5321..5449
                     /locus_tag="OUD09_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HCT9692849.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            5453..5998
                     /locus_tag="OUD09_001577"
     CDS             5453..5998
                     /locus_tag="OUD09_001577"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HCT9692850.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6452..8143)
                     /locus_tag="OUD09_001578"
     CDS             complement(6452..8143)
                     /locus_tag="OUD09_001578"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCT9692851.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8563..9510)
                     /gene="arcC"
                     /locus_tag="OUD09_001579"
     CDS             complement(8563..9510)
                     /gene="arcC"
                     /locus_tag="OUD09_001579"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCT9692852.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9625..>10637)
                     /gene="argF"
                     /locus_tag="OUD09_001580"
     CDS             complement(9625..>10637)
                     /gene="argF"
                     /locus_tag="OUD09_001580"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000195397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCT9692853.1"
                     /translation="SVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHYLEG
                     KNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRMFDG
                     IEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVAYCG
                     DGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDDVDK
                     GVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLPAFH
                     DTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNLFIP
                     RV"
BASE COUNT         2906 a         2208 c         1940 g         3583 t
ORIGIN      
        1 attaaacttc caataattct ttttctttat cagcagcgat cttatctact tctttgatgc
       61 tgttatctgt caatgtttgt acgtcttttt ctaatccgcg caaatcatct tcggtaatat
      121 caccattttt ttgttgtttt ttatattcat cgatcgcatc acgacggatg ttacgaatcg
      181 cgatttttgc attttctgct tcttttttca catctttagc taattctttt ctgcgttctt
      241 ctgtcagttg cgggataact aagcgaatga catttccatc attggttgga ctgattccga
      301 tatcacttgc cataatagct ttttcaacat cttgaagaat gctcttgtca aaaggtgtga
      361 tcattaaaac acgtgcttct ggaatttgga ttgatgccat ttggttcaat ggtgttggcg
      421 caccgtagta gttcacagta atacggtcca aaaggctagc gttcgcacgg cctgcgcgga
      481 tttgcccaag ttcacgttga agattttgtg ctgctttttt cattttttct tttgcttctg
      541 tcataattgc atctgccatt tttatttccc ccttacagtt gttccgatat tttcgcctaa
      601 gattgcacgg cgaatatttc cgtgttcatt taaattgaag acaagcagtg gaatatcatt
      661 atccatgctt agtgaacttg cagttgaatc catgacttgt aatcctttgg caataacttc
      721 cagatgagtc agttcttcaa attttactgc atttgcatct actttcggat cagcagaata
      781 aacaccgtct acattgtttt tagccatcag gataacatct gcgccgattt ctgccgcgcg
      841 taatgctgct gttgtgtctg tcgagaagta cgggttgccg gtaccgcctg caaagataac
      901 gatccgtcct ttttctaaat gacgttctgc tttacggcga atatatggct ctgcgatttg
      961 acgcatttca atcgaagttt gcacgcgtgt cggtacaccc acgttttcca acgtatcctg
     1021 taaagctaag gcattcataa ctgtagctaa catacccatg taatcagctt gagcacgttc
     1081 catgcccatt tgtgctccga tttgtccgcg ccagatattt ccgccgccca caacgattgc
     1141 catttcaacg cccagctcgt gaacttcttt gatttcttgg atgatttctt tgattactgg
     1201 tggtttgatt ccaaatcctg cgtccccagc taaagcttct ccactcaatt ttaagactac
     1261 acgctgatat ttcggtgtga ccatgttcat tcctccaata ttttctattg ccattttacc
     1321 atacttagat gatttcgaca ctctaagact ggtttttctt gttttgctta aaaaaaggga
     1381 gcgcacaaac cgtgcgttcc cgtctgttat tcaacagaat tattttttca cttggctcat
     1441 aacttcttct acaaagttat cttcgcgttt ttcgatacct tcgccaactt cgaaacgtac
     1501 gaatgtttta acagttgcgc cttttgatgc aacgtatttt tcaacagtca tatcaggatc
     1561 tttaacgaat ggttggtcca ccaaagcgat ttctgctttg aatttcttca agcgtccttc
     1621 taccattttt tcaacgatat tcgctggttt gccttcgttc aatgcttgtt ctgtaagaac
     1681 agttttttca tgttctaatt cagcttctgg aatttgtgat tcgtttacat aacgaggatt
     1741 gatagctgct acgtgcatag ctacgtctct tgcaactgtt tcgtcagttg ttccatctag
     1801 aacagtcaat acggcaatac gtccgcccat atgcaagtat ccaccaaatg cagcattgtc
     1861 gtctttttct acaacttcaa agcgacggaa gctgattttt tctccgataa cttgagttgc
     1921 ttcgatcaag tcagattcaa ttgtaccttt atctgttttg attttcattg cagcttccat
     1981 gtctgctggt ttgttttcag caacaagttc tgcaatttct tttactaaat cttggaacat
     2041 ttcgttttta gaaacaaagt ctgtttctga gtttacttca acgattgctg cagtgttacc
     2101 tttcacagca actgaagcca atccttcagc tgcaatgcgg tcatttttct tcgcagcctt
     2161 tgccattcct ttttcacgta aaagatcaac tgctttttcc atgtcgcctt ctacttcaac
     2221 taatgctttt ttcgcatcca tcattcctac gccagtcatt tcgcgtagtt cttttaccat
     2281 tttagctgta acgtctgcca tgtttaactc ctcctgtgtg tttttctttt taaaaaaagc
     2341 tgcttcaaag gagagtgagg cttgtcgccc agcctttgaa acagctccat cattcaaaat
     2401 tattctgctg aagaattgtc gccttcaaca acatctacga tttcttcgat tgatgttgct
     2461 gtgttttctt ctgtgaaatc ttcttcaact acttgatctt caccttggtt tccttcgatg
     2521 aaagcatcag ccatttttga agtaatcaat ttaacggcac gaattgcatc gtcgtttgaa
     2581 gggattacta cgtcgatttc atctggatcg cagtttgtat caaccatagc tacgattggg
     2641 atatttaatt tgtgtgcttc ttgaacagca atacgttctt tacgtgggtc aacgatgtac
     2701 attacatctg ggattcttgg catatcagcg ataccgccta agaatttttc aagacgttca
     2761 cgttctttgt tcaaaccagc aacttctttt ttaggtagaa cttcaaaagt tccatcttct
     2821 tccattgcat tgatttgttt caaacggctg atacgttttt ggatagtatc ccagtttgtc
     2881 aatgttccgc ctaaccaacg atggttaacg aagtattgtc ctgcacgagt tgcttcttct
     2941 ttgattgctt cttgtgcttg tttttttgta cctacgaata atgctacgcc gccttcttca
     3001 gcaacgctct tcatgtaatc ataagctgca tctactaatt taactgtttt ttgcaagtca
     3061 atgatgtaga ttccgtttct ttctgtgaag atatatttct tcatttttgg attccagcga
     3121 cgagtttggt gaccaaagtg tacgccggct tctagtaatt gtttcataga aattactgcc
     3181 atgtttgttt cctccgattt ggttttattt ttcctcttct tatcttctac cttgccggca
     3241 aactttttca agcacctacc gacaatccaa taagaatgtg gatttgctga tgcataaagc
     3301 accctcagct attatacaga caaaagatcc gtatcgcaag agcaaatata cttttctgct
     3361 caacattcaa tagtttacct gaaagataaa aagaattcaa gaaaaacagt agatttctct
     3421 ttgtttcttt attatccttt ttccataaaa taattttagg tataaaagac gaatattatt
     3481 gggcaatcct tctttttttt gttatgatac atgtgaaatc tttcgtaaag gagaaagaaa
     3541 atgcgcaaag ctcaaagaca tttgctgatt aaacaagtca ttgaagaatt cccgatccgt
     3601 acgcaagaag aactattaaa caaacttggg cattatggcg ttaccgccac gcaggcaacg
     3661 atttctcgag atatccgtga tctaaaaatc gtgaaggcac ctgatgaaaa tggcgtttcc
     3721 cgcttcgtat tgtttcgcga aaaaggaact cctgagacta ggactgagga agaggatcgg
     3781 ctggttcaaa tgattgaaga cattgtattg aaagtcgaac gagtccactt tttgacgatc
     3841 gtcaatacct tgccagacaa tgctcattta tttgcatctg ttttagatga gatcaaacct
     3901 ccctctattg tcagtacaat tgctggattc gatacgatca tggtcatctc ggaaaatgag
     3961 gaggacgcaa aacgagtaga agaatttctt caccatccaa ctgaagtatc acttttttaa
     4021 tcagagcacg tattcctaat atggaatgcg tgttttttta ttttcaatat tttccttatt
     4081 gaataaacct ttacactctt tacaaaaata catcattttt tattcaaatt tttcaacacc
     4141 ttgattgaat cacaataatc atcatttata ttggatgtta acaagagcag cttcttgctc
     4201 tttgtgtatt tcaaaaaaga aatgaaagga aatcatatgt ataaattatt aacttctgga
     4261 ccattgacga cttctgaaac agtgaaaaat gctatgctga tcgatcattg tacttgggat
     4321 caagaataca aagaaattac acaatggata agaaaaaaat tattggatct tgctcaagta
     4381 tcggaaggaa tatacacaac ggtcttaatg caaggtagca gctccttcgg ggtagaatcg
     4441 gtattaagtt ctgtgatcag gccagaagat actgtactga tttgttcaaa cggcgcatat
     4501 agacaacgaa tgatcaacat gtgcgaatgt ctgaaactga actataccgt ttatgaggta
     4561 tcggaacatc aaatcctcca agctcatgtg attacacagc tgattgaaga gaatccgtct
     4621 atctccgcac tagcaatggt ccatagcgag actacttccg gtattttgaa taatttggaa
     4681 gagattgctg ctgtcacaca agaaagagat cttttgttta tagtggacgc gatgtcaagc
     4741 tttggtggga taccgatttg tgtagccgaa ctagggattg attttttgat ttccagctca
     4801 aacaaatgta ttcaaggtgt tccaggcttt tcttttgtac ttgctaataa agcggaactg
     4861 gaaaaacgaa aaaatgttag ccgaagtttg tcacttgatc tatatgacca gtaccgcgtg
     4921 atggaaaacg agaatggcaa atggcgtttc acttccccta cccatacggt tttggctttt
     4981 caacaagcgc tgaaggaact agaggcagaa ggaggaatcg aaactcgata ttcacgttat
     5041 ctcgccaata atcatgtgct acgacaaaaa atgacagaga tggggttcca gcctttcatt
     5101 caagaatatc aggggccatt catcacctct tttctttacc ctaaggactg gcgatttgat
     5161 tttaatcatt tttatcagta tttaaagcaa aatggatttg tgatctatcc cgggaaaatc
     5221 agcgatcatg aatgttttcg tatcggaaat attggcgaga tctatccgga agatattttg
     5281 gaactgacag caattatcaa agactataag gagaacgtta atgattaaaa ctgtaatttt
     5341 tgattgggca ggaacaactg tggactttgg ctgtatggct cctgtccacg cttttagaaa
     5401 tgcttttcta gaaaaaggaa tccaactgac tgacaaagaa atccgctaac cgatgggaaa
     5461 attaaaatgg gatcatatcc aacagcttct ttccctccct tctgtcaaaa aacaatggat
     5521 agctatttat ggacaactac ctcaggaaaa agaggtggag gaattgtatc aacaatttga
     5581 acattatctg tttaaagatt tggtcaagca ttctaccttg aaacccgaca cattggaaac
     5641 gatcgaacgg ttgagaaacg ctgggatcaa tattggttcc acgaatggat ttaatgctcc
     5701 tatgatgaca atcgtagcag aaaccgcaaa aaaagaaagg tattcaccag acttcatggc
     5761 aacttttgaa gacgtcgaag ggtatggtcg accgtttcct tatatgatcc ataaaaacat
     5821 gcagtacttc aaaaacaagt cggttgatga agtaatcaaa gtcggagata cggtctcaga
     5881 tatccaggaa gggaaaaatg ccggagtgtt gacagtaggt gtaattgaag gtagttcgct
     5941 aatggggatt tcttatgatg agtaccaaaa attagagatg gggaaaaaga tcgtttaaaa
     6001 cagtctgttg ctcttagatt ccaacaagcc ggcgctgata tttgtgtaaa caatttaaaa
     6061 gagttaacag aatacatttt gtacgctgac aagaatccag aatgactttt atacaaaata
     6121 agaggttgtg aaaaagctgt cctgtatcaa gtaataaaaa cagccaagca aaaatagctc
     6181 ctcatatttt tcgtttggtt aggcttatta ccgcagatgc aagcttttga acaccgttta
     6241 ttcgaaaaaa aaaagggagc tgtgacacga cttttgtcac agtcccctta tttgcaaaca
     6301 aacagtgttt aagagacaac ctctccacta aacggtgttc attcagctga ctctcggtat
     6361 ttgctcaaaa aacgataaaa tttgaaaagc tattttctct tttgtttctc taatactcgg
     6421 atcagaacgc tgatttcaca ttctcttttt cttacatctt atctggtgca tgaagtccta
     6481 gcaaacgaag gtcttctttc aataatacag tcactgcata aactagagct aagcgtgctt
     6541 ctttttgttc atcatcagct aaaatcttcg tatgagcata gtatttgttg aatgcttgtg
     6601 ctaatttgat tgcatgtttc gcaatcactg acggttcgta tttttcccca gcagacaaga
     6661 ccgtttcagg gtatttttga actaatttaa ccacttccca gctggtatcg tcattcaatg
     6721 catagtctgc tgtttctgaa gggacaaatc ctgctttttc caacaggctg actgcacgag
     6781 catgtgtata ttgcacataa ggtccagttt ctccttcaaa tcgaacaact tcttccaggt
     6841 taaagtcaaa tgtattcagt cgatcgtttt tcaaatcgtg gaaaatgact gccccaaccc
     6901 caacttgttt agctacagca tctttgtttt ccaaatcagg gtttttctcg ctgatttgtt
     6961 ctttagcaga ttggattgct tcatttaata cttcttctaa taagacgatc tttcctttac
     7021 gagttgataa ttttttacca ccttgcgtga tcaaaccaaa tggaatatgg tgcatgtcgt
     7081 cagaccagtc aaagcccatt tccttcaaga cagctttcaa ctgtttaaaa tgatagcttt
     7141 gttcattccc tactacatat agagattgtt tgaagtcgta agtacgttta cggtacaatg
     7201 cagcagctaa gtcacgtgta atgtatagtg tcgcaccgtc tgacttctta atcaacgctg
     7261 gattcaggtc ataagcagat aggtcaacga tctctgcacc tttatcctcg ttcagcaaat
     7321 gtttttcttc taaaagtttg acgatttcat ccatcttgtc attgtagaaa gcttcaccgt
     7381 tcaacgaatc gaaacgtact tccagcagat catagatttt attgaattct ttcatcgatt
     7441 catcacggaa ccattgccat aattgaatag cctctttatc gccttcttcc agacgtttga
     7501 accaagcacg agcttcttca tttaattctg gttctgtttc agctacttca tggaattgga
     7561 cgtataaacg aagcaattca ttgattggtt ctgctttaac agcttcttct gtaccccatt
     7621 ttttgtaagc gacgatcagt tttccaaact gtgttcccca gtcacctaaa tggttgatgc
     7681 ggattggctg ataaccgatt ttttccataa tgaaaccgat cgaattaccg ataactgtcg
     7741 aacgcaaatg tcccattgag attggtttgg caatattagg agaagacatg tcgattggta
     7801 ctgttccttg attcccgatg ttgctatccc cataatgttc tttttctttc actacggttt
     7861 gcaaaacttt tttgctgata agttctttat tcatgaaaaa gttcaagtaa ggtccgacaa
     7921 cttcgatttt ttcaaagttg gcagaatcga ttttttctgc taaatctgca gcgatttgct
     7981 gtggcgcttt tcggtaaact tttgctaatg aaaaggctgg aaatgcaaca tctccgtgtt
     8041 cagctgattt tggattttct agtaattgct cgacttgttc cagtgttaaa tcatctttga
     8101 cagcgtcata gacagctttt gcgacaagat ctttattatt catctttatt cctcctaaag
     8161 tgttcccttg attgttaaac aaaaaaaatc tctagcgacg gcatacttag tatgccatca
     8221 ctagagacga gatttcccgc ggtaccactc caattgtcct aaaaaaggac cagctccatc
     8281 tgatatcggt agacgaccgt ctgtttaaaa cagaattctc caagtgcgct tcgttgtatc
     8341 ttcttcattc ggcttccacc acttccgaat tcgctagcca agaatgatac aagtactctc
     8401 ttgttcaaca aatcttagtc ttattaaact ttcttgaatt atatcagaaa aaaaaacgaa
     8461 aggctagtcg tttcataaaa aaaaaatcca gatggcctaa aggagaagaa ataccacctg
     8521 gatagctata aatcatttcg taaatacgaa agacttaaca aattaatcag ctacgataat
     8581 cgttccttct tcagaagcta aaagattctc gatattttct aaagaagtga tgattgcctt
     8641 agcgtttggt tttgcttcaa caaatgcaat agctgcttct actttcggaa gcatgcttcc
     8701 tggagcgaat tgcttttcat caatgtattg tttcatttct gaaacagtaa cagtttctaa
     8761 tttcttttga tctggttttt ggtaattgac atacacattg tctactcctg tcaatacgat
     8821 cagcaaatcc gcatcgatga tttcggctaa tttttctgaa gcaaaatctt tatcgattac
     8881 ggcttctcgt ccttctaatc cagtagctgt ttctacaaca ggaatcccac cgccaccaac
     8941 agagactgtg ataactcctt gatcgaccag cgtttcgatg acacgtgctt ctttaatcga
     9001 gatgggttta ggggaagcga ctacttttct ccatccacgt ccagcatctt ctttgaatgt
     9061 cacagtggag tcagcattca tttgttcttt ggcttcttct tccgtgtaga atggaccgac
     9121 tggtttactt ggatttttga atgaaggatc attttcatcc acaataactt gagtgactaa
     9181 agaaaccaca tctttatcga tccctttttc cttcagtact tcgcccatgg cgttttgtaa
     9241 ccagtaaccg atagatcctt ctgtcattgc aacacatgta tctaaaggca ttgccggtgt
     9301 tttctcagaa tcagcagctt gctgctgaat caataggtta ccgacttgcg gaccatttcc
     9361 gtgggagatg atcaattcat ctccttgttc gataaatttc actaaatact ttgctgtttc
     9421 cattaacgct tcttgttggg cttttgcact tgcatccgta gacaagatgg cattgccccc
     9481 taaagcgaca actacttttc gtttggccat ttcattcgtc ctttcttcag ttctaccgaa
     9541 ttttctagat aacaaaacga gccagcgttt tgtgattttc cctcaatgct gactcgtttt
     9601 ttcgtgagat gaatgtggtt atgcttaaac gcgagggata aacaagttac ctaatgttgc
     9661 tgccatgatc gctttgattg agtgcatacg attttctgct tggtcaaatt gacgagcata
     9721 tttgctgcgg aagacttcat ctgttacttc catttcagtg atgccaaagc gttctttcat
     9781 ttgttcaccg taaacagtgt ttgtatcatg gaaagctggc aagcagtgca agaagatcaa
     9841 acgatctgtg ttgtgtgttt tttctaccat ttccatattg atttggtaag gtttcaacaa
     9901 tttgatacgt tcttcgaatt tatcttcttc acccattgat acccatacat cagaataaag
     9961 gacgtctgct ccgtctacac ctttatcaac atcgtcggta atcatcaatt gtgcaccgga
    10021 tttttcagca aagccttctg ccattttgac gatttcttct tcaggttgta attcttttgg
    10081 tgcaacgata cgcatattcg ctcctaaaat tgcacctgtg actaataaag agttagccat
    10141 attgtttcga ccatctccgc agtaagcaac agtgatacct tctactgttc caaagttttc
    10201 ttggatagtt aaaaagtcag cgatcatttg tgttggatgc cattcatcag tcaagccatt
    10261 ccaaactgga acaccagaga attctgctag ttcctctacc attttttgac tgaatccacg
    10321 gaattcgatt ccgtcaaaca tacgtcctag tactttagct gtatcttctg tggattcttt
    10381 tttacctaat tggatatcat ttgcacctag atattcagga tgtgcaccta gatcgatcgc
    10441 tgcagtagta aatgcagaac gagtacgtgt agatgttttt tcaaataaaa gcgcaatatt
    10501 ttttccttct agatagtgat gtgggatacc acgttttttc aagtctttca agtgttctga
    10561 aaaatcgatc aaatattgta attcttcttt tgtaaagtct ttttctgcta ataagcttct
    10621 tccttggaat actgatt
//
LOCUS       DAKBVG010000089        10601 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000089 DAKBVG010000000
VERSION     DAKBVG010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.089"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(<1..998)
                     /locus_tag="OUD09_001581"
     CDS             complement(<1..998)
                     /locus_tag="OUD09_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCT9692854.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYERILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYL"
     gene            1029..1250
                     /locus_tag="OUD09_001582"
     CDS             1029..1250
                     /locus_tag="OUD09_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HCT9692855.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(1415..2152)
                     /locus_tag="OUD09_001583"
     CDS             complement(1415..2152)
                     /locus_tag="OUD09_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCT9692856.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGNSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(2306..3646)
                     /locus_tag="OUD09_001584"
     CDS             complement(2306..3646)
                     /locus_tag="OUD09_001584"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HCT9692857.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(3883..5406)
                     /locus_tag="OUD09_001585"
     CDS             complement(3883..5406)
                     /locus_tag="OUD09_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HCT9692858.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(5784..6395)
                     /locus_tag="OUD09_001586"
     CDS             complement(5784..6395)
                     /locus_tag="OUD09_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCT9692859.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIP
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(6518..6642)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(6724..7440)
                     /locus_tag="OUD09_001587"
     CDS             complement(6724..7440)
                     /locus_tag="OUD09_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HCT9692860.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(7446..8015)
                     /gene="scpB"
                     /locus_tag="OUD09_001588"
     CDS             complement(7446..8015)
                     /gene="scpB"
                     /locus_tag="OUD09_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HCT9692861.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISFEEDVSPDLFFDGMNKGE"
     gene            complement(7999..8796)
                     /locus_tag="OUD09_001589"
     CDS             complement(7999..8796)
                     /locus_tag="OUD09_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HCT9692862.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(8766..9137)
                     /locus_tag="OUD09_001590"
     CDS             complement(8766..9137)
                     /locus_tag="OUD09_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCT9692863.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGVYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(9156..10043)
                     /gene="xerD"
                     /locus_tag="OUD09_001591"
     CDS             complement(9156..10043)
                     /gene="xerD"
                     /locus_tag="OUD09_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCT9692864.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(10139..10588)
                     /locus_tag="OUD09_001592"
     CDS             complement(10139..10588)
                     /locus_tag="OUD09_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCT9692865.1"
                     /translation="MKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSAEEIFFLVK
                     KKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHLLCLNCGTI
                     EEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
BASE COUNT         3048 a         2285 c         1777 g         3491 t
ORIGIN      
        1 aatagataga tttgatacaa acgaaattct ccataatcta attgtccatc tacattccat
       61 tcgctaaagc gccactctcg cacggaatcc ttatttggtt ccaaacgttc gattgtttct
      121 gcagagagaa tccgctcgta tggaaaatca tcaaaaaaca aggcccactc aataagatga
      181 tctgttactg ttcctctttt taaatgacgg attgccataa tctggtcaat tgtctgacca
      241 ttcataaaca tctgcttggt gatcatcatg ctttgattga aattttgctg taataatgaa
      301 tcgatcaaag cgtgccaaag cgaatctggc cgtttttcga tttgtgctaa caaaaggtgg
      361 atacatctgc tttgttgcag ataaaccgct gactcatcat tgttttttgc cagctgataa
      421 ggaagcagcc ctgttcgctc atttccagat aattggttag ctaaaaaatc tgctgcttct
      481 tctggtatct ccgaaaacag ctgcaccaaa ctctcatacg cagtatcaat caaaatttca
      541 cgtggcaatt ggctttcact aagccatttt tttagttgga aagtataaaa aggacttgtc
      601 tctgctggaa gatagtcttg aatacccgtt gccagattag aaattacttg aacagcaaat
      661 ttaatgagcc gccaactagt ttcccctgtt ctgccatagc ggtcaaaacg caaaccagta
      721 tgttctattt gatgttcgga caacatatca gcccctgcag gcgttagctt tgcttcattt
      781 tcatttattt cgatccaacc ttgctgcgca attttttgca aaatttgata aaacttatcc
      841 tgcttcaatt ctggaaaact gttatggata aacaaaagtt cgtgggtaaa tccataaata
      901 agtactgaag tcgttcgttt tcccacaagg agttggtaaa gagaactaat ccgcagcttg
      961 tgtcctgatg aaaatagcga taaaataaaa acaatcatat tattctcctt aagttaggat
     1021 ggttagaaat gtcactacta tgtaaacttg tacctgaacg ctgtattgca tgcggtcttt
     1081 gtcaaatcga agcgccagat attttcgatt atgatgaaga aggaatcgtt ttatttgccc
     1141 aagaaccaga ggctcatcaa cagttcgtga tgccggaaga agaaacatat gtaaaaaaag
     1201 cgtatcaaaa atgtccagtt cgagcgattt tattagaaaa taaaatataa atataaacga
     1261 aactttaaaa acgtacacta agttctctaa acttgaaaat aagccgggat ctgtttctct
     1321 tgttccgtga aatcccgact tattttgaaa gattcatttg ctatctatag ttaattgact
     1381 agttttttca cggaccctat aggacccatt tatattattt caactttttc agccagtttt
     1441 ccaatgtatt gacgatcaca gtttgctggt ctgcgttaga aatttctgcc tctccatctc
     1501 ctttttgctc accatagctg ccaaaacctc catgatttcc gccattgatt gatacatact
     1561 ctgttctttc cggtaaatac tgcttagctt cttgataagc ttcctgattc aatactttat
     1621 cttttgtcgc cgtgatcgat aaaacaggaa cagctgattc tgtcaatgtt ccttttttgt
     1681 cgggataact tgccatgaag aatacccctt ttaaatgttt gtctgaatga ttcttattga
     1741 cataacgact tgccattacg ccacctaatg aatggccacc gatcacataa tcggaatttg
     1801 gaaaatcttc tttgacctta tccgctttgt ttccgcccat cactgctaga tcaagtggca
     1861 ttttgacaat agcgactgaa aagccgtttt ttgctaaatc ttgcgcccaa atactataac
     1921 tttctggctc tacgagagca ccaggataaa aaataagtaa tggagaattt ccatgattct
     1981 ttttctctcc ttcaaaaaat aagtaatctt ctttgttttt tccttctttt accaacactt
     2041 gcgcttctga cgatggttcg tacgtatttt gctgtatata gtaataaccg gctccaccga
     2101 gtaatcctaa aatgatgact aagccaatta gtattcgtct aaatattttc actttctccc
     2161 accttttcta tctgagtgac ccctttttct taatcaagta tgattatacc ttaaaaaaaa
     2221 taagagagaa atgcaaactt tccagcatca aataaaaaaa gacggaatat ccatttgaat
     2281 atccgccctt tttgtatgtt tatttttatt tagcacataa acgatacaca gcttctgcat
     2341 aaatttccat tgctttatac atactactta attcccatcg ttcattcgcc tggtgcataa
     2401 agtcaggagt atcagggaac atcgcaccga atgccacaca ctggttcatc gtacgagcaa
     2461 aagtagcgcc gcctgataca aacggttcag tcatgtctcc agtaatatcg cgatatgtcc
     2521 ccaatagatt tttcactaac tcactgtcta gtggcacgta taatgaatca aggaagtcaa
     2581 attcttcata agtcaaccca tatttttctg ctgttttagt taatttagct actaaatcat
     2641 ctttcttgaa tgtaacaggg attctcatat ctacaccgat ttttgtttgt tctggtgtga
     2701 tttccaaact tgcaaagttc atcgttagct cgccggattg ttcatcttct gttttgccca
     2761 cgacagcttc accagttgca ttttcttgaa ctaattttcc taagaaattg attgggccaa
     2821 aatcgaatac ttctgaaaga gcaattgcca gacgagaaat agcattcact ccttgtgctg
     2881 catctttcgc gtggatactt ttaccaagaa cgacgatgcc ttcaccttgt tcttcgaaat
     2941 cgaattcgtg ttttttcaac gcttctttta cttcagaaag cttttctcca gagtaaggag
     3001 ctgcatcagg aacaacattc aacgctcctc cagcttttac agaaaactcg ttcgttcctg
     3061 gtcctgtcaa gtaagcttgc aataagcctt tttctgcata aattaaagga aattcagcat
     3121 ctggcgcaaa tccttgcgta attccttctt ctttttcgtt atatttttct aaacaacgcc
     3181 ataaattttc ttcatctgta ccaaagataa aacgaatacg tgtcttgaat tccacgccag
     3241 catcaatcaa agctttgacg gcatacattg ccgcaataga aggacctttg tcatcttggg
     3301 atccgcgacc aactaaccag ccgtctttta tagttggatc gaatggtttt gtttcccaat
     3361 tattttcgtc ccctgctgga acaacgtcca tatgacacaa gataccgaat aattcatctc
     3421 cagagccaat ctctgaataa ccgtaatagc cttctggatc tttaaatgtt ctaaatccta
     3481 gcttttcact gatttccaat actttatcca aagcttcaat cactttttta ccgaagggat
     3541 gtccttgtcc tgaatctgct tcatctagta cggaaggaat tcggatcaat tcgctcaatg
     3601 attctaatgc ttgttcatga tgttgttctg tcacaaattt tttcatgata ttatcctcct
     3661 aatatgttaa aaagggtcat gtcaaattcc tccgacacaa cctcttcttc tcaaataatg
     3721 acgttttaag cctgctccat cagcaagcag aatgttctta acaaatgatg aaagaaaaaa
     3781 cactcactga atggagctat tgacttgtcc acgtgcttat cgatagccgt ttatcggttt
     3841 cttcattctt atgatagacc ttgtacctgt atttgtctag gtttagaaca ttgctgctac
     3901 acctaggatc acacagatgg ctacaaataa tatcgcaatt aatttaccgg taaatttcca
     3961 ccacactgaa acatcgaaac gagcaattgc tacagcaccc attacgatag cagatgttgg
     4021 tgtaattaag tttactaaac cagatgcaga ttgataagca gtgattacta aatccttacc
     4081 gacgccggca aattcaccca taggtcccat gattcccatt gttgcagcag ctaagccgga
     4141 agttgaagga atcaagaatg acatagggat atagaaaata aatgtaataa tgatgaatac
     4201 acttgaagat aatgaactta atccttgttc gccccaatgt aagatggtgt ctgtgattaa
     4261 tccattgttc ataaccactt ggataccacg agcaacagct actacgattg ctacaccgat
     4321 caaatcagaa gcaccagcta aaaattcaga gacaaattca gactctttca tgccataagt
     4381 gattgctact acgatggaca ttactaagaa taacatagta atttctggga agtaccagtc
     4441 acctaatgga agcatgcttt tacctagaac agtacctagt accggaaggt ttgttaacca
     4501 gtttgtcaat gattcaaaaa tcgtgaaatt cgcattcaag gttgtccaag gaatcaaact
     4561 gatgatcatg ataccaaaag taataaagaa caatacatta acatgttttt gttttttcgt
     4621 gattacttct tgacgaccga ttgcttcaat atcgaaatgc ttcatatctt ctttacgttg
     4681 gttgtatact aatgattttg ttggatcttt ttcgatcttt gatgcataac gatacacata
     4741 tacaatagaa atagctaaca aaataacgaa catcaagaat cgaagaccaa taccttcacc
     4801 catcgaaatc ccaacagctt gtgaagctac gcctgttgca aaaggattta ccgttgaagc
     4861 tagacagcct acttgagacc ctactaatac gattgctact gctactactg tatcaaagcc
     4921 tacagccatc ataactggga tcagtaaagc atagaatgga atcgtttctt ctgccattcc
     4981 gtaagtagag ccgcctaatg caaataacag catcaatact ggaataagca ttttttcttt
     5041 tcctttgaat ttcttcacaa ctgaagcaat cccggcatct agtgcccctg ttttgttgat
     5101 aatacctaaa aatccaccaa caaccaaaat gaagaaagag attgggattg cagcaggtgt
     5161 ttctttagtt agctcccccg ctggtacacc taacatccca ttgattggcg ccatgaaaat
     5221 atcccacagc ccttgaggat tttgttgtac tcgttcatat gatccagcga cgatgttgcc
     5281 tgcatcgtct acactatagt gcccagctgg tataaaccaa gtaagtacag cgattactgc
     5341 aataataata aataagaccg tatacgctga aggcatttga aattttttct tttttgtttg
     5401 ttccatcttc ttcttccttt cttttccgtt agagacaaca attttggctc taatgttatt
     5461 ttatcaaaaa gagtatcaga acaaaaataa taacacgcat tttgtaaaac ttttgttcaa
     5521 aaagttcgtt tttccctatt ccctcctctt ttttcaggtt tttaaagctt tcttcttttt
     5581 cattgtagcg tttccttttt tttataaaca aacattttcg tcttttaaaa ggagaagaat
     5641 ttcacatttg caaagaattg attgattttt atgcataaat caaaaaaata ctcaaaacaa
     5701 acaaataaaa cgtttacatt attgcatgtc ttgtattttt ttccaaaaaa aaagaccaag
     5761 aaatttcttg atctttttga taattatttg actgctgtat gacgttgttc gaactgatgt
     5821 tgttttcgac ttaaccatgg aagaagttgc gcatgcagca ctaaaaagac agcgctcacg
     5881 atgaatcctt tgatcaagtt gaaaggaacg atcccaatca gtacatagtt tgctaaagac
     5941 atgcctagca tctgtgttgc gttcagtcct aaaaacatta agtaaagagg cgtaataacg
     6001 aaataatttg cgatactcat aaaaatagtt agagcgatgg ttcctgtaat gacacctaaa
     6061 aatttgtttc ttcctttatg ctgtttcttt acactaaaga aataataaat cgggaatgta
     6121 aagatcgttg ttgataagaa actagcagtg tctcctacca aattatccgg agaaaaacct
     6181 gtagtcagca gatgtaatac tgaacgcaag aacgctgttg caactccggc cgctggaccg
     6241 aataagaaca tgctgatcaa taccgggata tcactaaaat cgatcttcaa aaaactaaaa
     6301 gccggcatga tcggaaaagc aaaatactgt aaaactaacg cgatcgcagc aaacatcgct
     6361 actgcaacca ttttctgtac acgggtgttc ttcatttgaa atcctcctgt taggactcct
     6421 cttcagcgaa ctttttgacg tttctctcca aatgaaaaaa ctctattttc cagggaaaat
     6481 agagttagag ttatttgatc aagcgtcgtc aaataagctc tatcttcttt atccagacta
     6541 tactgtcggt atcggaattt caccgattca gctatttttt gcaaaatagg tcgtggacta
     6601 taaccaccgg tcgggaattt caccctgccc ctgaagacga accttatata atttttgttt
     6661 acatattcat tatacatatc tattcaaaaa acgcaacatc taacacttat aaaaagtaag
     6721 tgttcatttt gagtggttca gcaaactgct gacttctttt ttgttcagtt cacggtaatc
     6781 tccaggacgt aggccttgta aagtcagatc tccgtatttt tcacgtttta gtttttgcac
     6841 aggaagacca actgcttgaa gcatattttt cacttgatga ttacgtcctt cgtggatcgt
     6901 caattccacg atactgtggt ttgtcttagg atccactgat aaaatttgga atttagctgg
     6961 agatgttttt cgtccttcga tctttattcc ttttgtcaaa ggtgcaagca tcgattttgt
     7021 agcgatccct tttacctttg caacgtaaac tttatccact tcatgttttg gatgtgtcag
     7081 gcgctgagca aaatctccat catttgtcag tagcaagatc cccgaagtat cgtaatccag
     7141 tcgacctaca ggatagatac gttcttttac catcggcaaa agatccgtaa caacttttct
     7201 tcctttgtca tctgcaacag cagaaataac tcctttaggt ttgtacaata aataataacc
     7261 gtattcttct tgataaattg ggacttcatc cacttccaca gtgtcatgtt tcccaacttt
     7321 tatccctaat tcccttacaa cttgtccgtt tacttttaca cgtcctgcaa ggatgaattc
     7381 ttctgctttt cgacgagaag tgatgccagc gtgggcaatt actttctgca atctttccat
     7441 tacaattatt ctcctttatt cattccatca aagaatagat ctggtgacac gtcttcttca
     7501 aatgaaattt ccatttcttg tgtatcaggc agttcttcta atgatttcaa accaaaataa
     7561 tctaagaagt agccagttgt tccatataat atcggccgac ctggcccttc tactctacct
     7621 ttttcttcaa tcagtttatg ggcagctaga cgctgtaccg ctcctgaaac ctgaactcct
     7681 cgaatctgct cgatttcagc tcttgtgatt ggctgtttat aagcgatgat tgctaaggtt
     7741 tcgacagcgg cttgagataa tgtgtttgat gtcccttgcg catatcgctt aagtaaggga
     7801 gccagctctt ttttagtcgt taatacataa tggctttccg tttcaaaaat atgcaaagct
     7861 gagtgcgagt tttgagtgta agattctttt aattctttca ctaaacttgt gacttctgct
     7921 tcgctgagat ttaacagtcc gcttaattct tcattactga tcccttcctc gccaacaata
     7981 aaaagaaggg cttcaagttc acttaatttc gtcatgttcg gtatctccta ctgctggata
     8041 aagcataatt gattcatagt tttctttttg ttgcgcaatg atctgccctt ttttcatcaa
     8101 ttctagcaaa gccataaaag tagtgacaac ttcactttta gaaggtgact caaaaaaaga
     8161 atcgaaagta cagccatgcg catttctttt tatacgctct tcgataaata ccattcgttc
     8221 ttcgatcgtt gtgtcatcgg gcgtgatcgt tttttccact ttttgagaaa atttcttacg
     8281 ttctaagatt ttctgcatgg cacggatcaa atcgattttt ttgacttgtc ctcgcttcaa
     8341 cggcttttca gcatccgcta acccatcgat gttcataggt tcttttgtgt aataaagact
     8401 tcgttcctct gctttttctg tcagtctttc agctgcatat ttatatttac ggtattctaa
     8461 aagctgagtg accaattctt ctcgaggatc ttcttcccat aattcttcat catctgtgac
     8521 gaattcttgg acaggaagca atgtcttgct tttgattgcc atcaatgtag cagccatcac
     8581 taagtattct cctgcaacag ctagctctag tgttttcatt gtatggatat aattcatata
     8641 ttgctccgtg atcgtagcga tcggtatatc atatatatcg atttccattt gctgaatcag
     8701 atgaagcagc agatcgagag gaccttcgaa tacatctaat ttaatattga tctccgtcaa
     8761 ctcattcact ctttcttctt tttctttgct agccattttc caatgtaagg ctctgtgatt
     8821 tcagttgcac ggatggagac atccgggtac ttttcctgta attcatctaa catttgaaag
     8881 cccatattct caccgcgata agacggattc aaagaaatgt catggatgat cagctgatcg
     8941 ttcgttgccc agtaaacacc aataatccct tgaatgttgg ttgaaccttc tggcgtaaag
     9001 aaaaaaagtt cataatcgtc ttcagattcg tacgtatcaa tctccttcaa caaagaagtt
     9061 ttacctgtca gtttcttatg gaaactcaat agtcccatag caattttacg gttttctttc
     9121 cggtaatgtg ttaacatctt ctcaccttcc tatgactaag cccttggaaa atgctgctta
     9181 taaacatccg tcattctttt cttcgtgata tgtgtataaa tctgagtcgt agagatatcc
     9241 gcgtgaccta acaattcctg tactgtccgc aaatcagctc cattttctag cagatgtgtg
     9301 gcaaagctat gccgcagcgt atgtggtgtt acttctttgt aaatcccagc ctctcgtact
     9361 agctgcttca gatttttcca gattccttgc cgagaaagac ctttcccatg attattgaca
     9421 aatacatgca tttcagatgc gtcctttaca agtaatggac gtgcttcgtc taagtagcgt
     9481 tccagccatt ggatcgcata atcacctaaa ggaatgatcc gctctttatc tccttttcca
     9541 actgtctgta ccagtccaag agaaagatga agatctccca atttcaatcc aatcaattca
     9601 ctgacgcgca tccctgtagc atacatgact tccaagattg cacgatctct tatccctaac
     9661 gtttttgtcg tatcaggtgc ttcaatcagc ttttctactt ctgtcagagt caatgtactt
     9721 ggtaatttct gtgctttctt cggcgtatcg atatgttgca tcggatcatg atcagtgata
     9781 cgctcttgcc gtaaaaactg atggaatctt ctgagacttg agatcatccg agtaacagtt
     9841 gctgtcgctt ttttttcttc atgcaacttc tccagaaaac ggatgactaa aaagcgatcg
     9901 acatcctgcc atgaagcaat tttctgctcc tgtagaaaat gcaagtactg ttccaaatca
     9961 cgttcatagc tttgtcgtgt attggcggac aatccccgtt caatattcaa gtagtggaga
    10021 taatcgttga tttgctcttt cataataatc cccctcatgc ctctctatca tatcagatac
    10081 cgcttcattc tgatagtctt tttccataat ctgagcaaag aaaaagccgt ctagacactc
    10141 agacggactg gtttcttttt tctttttcat agcattcctg acagattcca tggaacgtta
    10201 gccgatgatc ttttactaaa aaatgataac gttcttctac tacttgctcc acttcaagta
    10261 aaagatcctc ttctacttcc tcgatcgtgc cgcagttcaa gcatagtaaa tgatgatgga
    10321 aatgtttggc gccttctttt ctcaaatcat aacgggctaa tccatcatta aaacttactt
    10381 tatcgacaat tttcaattct gtcaagattt caagggttcg ataaacagta gcaagtccaa
    10441 tttccggact cttttttttc actaaaaaga agatttcttc tgcagataaa tgctctttct
    10501 cattttccaa aagcacaagc agagtagctt ctctttgcgg tgtcagctta aaccccgctt
    10561 catgtagctg ctgttttgtt tttttcatcg ccgaaatagc a
//
LOCUS       DAKBVG010000090        10596 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000090 DAKBVG010000000
VERSION     DAKBVG010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10596
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.090"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            73..975
                     /gene="gnd"
                     /locus_tag="OUD09_001593"
     CDS             73..975
                     /gene="gnd"
                     /locus_tag="OUD09_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HCT9692866.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            977..1978
                     /locus_tag="OUD09_001594"
     CDS             977..1978
                     /locus_tag="OUD09_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HCT9692867.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            1975..2259
                     /locus_tag="OUD09_001595"
     CDS             1975..2259
                     /locus_tag="OUD09_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HCT9692868.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            2292..3089
                     /locus_tag="OUD09_001596"
     CDS             2292..3089
                     /locus_tag="OUD09_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HCT9692869.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            3103..3936
                     /locus_tag="OUD09_001597"
     CDS             3103..3936
                     /locus_tag="OUD09_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCT9692870.1"
                     /translation="MEILSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            3954..4811
                     /locus_tag="OUD09_001598"
     CDS             3954..4811
                     /locus_tag="OUD09_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCT9692871.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            4833..5342
                     /locus_tag="OUD09_001599"
     CDS             4833..5342
                     /locus_tag="OUD09_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCT9692872.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            5379..5780
                     /locus_tag="OUD09_001600"
     CDS             5379..5780
                     /locus_tag="OUD09_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9692873.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            5892..6245
                     /locus_tag="OUD09_001601"
     CDS             5892..6245
                     /locus_tag="OUD09_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9692874.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            6279..6722
                     /locus_tag="OUD09_001602"
     CDS             6279..6722
                     /locus_tag="OUD09_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCT9692875.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            6739..7824
                     /gene="uxuA"
                     /locus_tag="OUD09_001603"
     CDS             6739..7824
                     /gene="uxuA"
                     /locus_tag="OUD09_001603"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCT9692876.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            7817..8530
                     /locus_tag="OUD09_001604"
     CDS             7817..8530
                     /locus_tag="OUD09_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCT9692877.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            8859..10160
                     /locus_tag="OUD09_001605"
     CDS             8859..10160
                     /locus_tag="OUD09_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HCT9692878.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
     gene            complement(10238..10387)
                     /locus_tag="OUD09_001606"
     CDS             complement(10238..10387)
                     /locus_tag="OUD09_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9692879.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
BASE COUNT         3537 a         1738 c         2308 g         3013 t
ORIGIN      
        1 aatatctttg taaaatctat ttacaaaaaa atttttttgt ataatgaggg tataagaaat
       61 ggaggcgcta tcatggaaat tggtgtaatt ggtttgggaa aaatgggatt gaatttagct
      121 ttaaacttaa aagagcacca tcacaaagta caaggactcg acatttcaag tacagctgtt
      181 agtcaggcaa gagaaaatga cattcctgct tatcaagaga tctctgaatt tctagctact
      241 tttgagcaga aaaaaatcat ctggttaatg cttcctgctg gagagataac cgaaaacatg
      301 ttaagaaaac tttttccaaa actcggacca ggagacatta ttattgaagg cggaaactct
      361 tattataaag attcaatccg tcgagcagaa gagtttcaga aaaatgaaat cggttacttg
      421 gattgtggga cttctggagg aatcgaagga gcacgacatc aagcctgctt aatgattgga
      481 ggagataaag aaacttttct atccgtggaa caattattca aagatgtagc aattgaaaga
      541 ggatatctgt atgctggtgc atccggaagc ggacacttct tgaaaatgat ccataatgga
      601 atcgaatatg gaatgatgca agcaatcggt gaaggattcc aacttgtaca aaaaagtgag
      661 tatgcttttg acttggaaca agtcgcacgt gtttggaatc atggttcggt gattcgcggc
      721 tggctgatgg aaatcatgga agatcagtta aacgaacatc ccaatttaag taattatcgg
      781 ggaatcgttt ctgcatcagg tgaagcaaaa tggacggttg aaacagcgct agaaatggct
      841 gttccagttc cagtaattgc aactagtttg ttcatgagga atctttcaca ggaagaagat
      901 agtttttcag caaaagtagt ttcagcacta agaaatgggt ttggcggaca tgaaatagtt
      961 gaaaagggtg agtaaaatgc cagattatca tatagcaatc gttaattcca gcagtttcgg
     1021 aagagtcttt ccacaccact tggaaagatt aaagtcagtt ggagacgttc atttctttac
     1081 tgttgatcaa gagattccag gaaaagaact agcggagtta cttaaaggat tcaatatcat
     1141 tattgcaagt gttacgccat tttttcgacg agattttttt gaaaacaaag atgaactgct
     1201 attgatttcc cgacatggta ttggttacaa caatattgat ttaaaagcag caaaagaaca
     1261 ccagacaatc gtatctatca tacctgcttt agttgaacga aatgctgtag cagaaaataa
     1321 tataactaat ttgttgtcca tgcttcgacg cacgacacaa gcacaagcaa gagtcaagga
     1381 cgatcgatgg gaagatcggg cggagttcgt cggtaggacg ctgtttaaca aaacagttgg
     1441 tgtgattggt gcaggaaata ccggaagcgg agtaatcgaa attttgcgta acggttttcg
     1501 ctgtgatgtt ctagcttatg atccttacaa atcagattta tacatcgaaa gctttggtgc
     1561 aaaaaaagta gaacttcaag aattactgga acaatcagat atcatttgtt tatgtgcgaa
     1621 tttgacagaa gagaactatc acatgatttc tgataaagaa gtcgatcaga tgaaagaaaa
     1681 tgtttatcta tctaatagtg caagaggcgc tctagttgat gaagaagcga tgattaatgg
     1741 tctgaaaagc ggaaagatcg caggatatgc cacagatgtt ttagaagaag aaccgggacg
     1801 tcaagatcat ccgtatttag cgtttgaaca agtagtgatt acgccacata tatcggccta
     1861 cacaagggaa tgtttagaag caatgggtga aaaatgtgtc agagatgtcg aacagatcag
     1921 tcagcacctt cttcctgaac gttctgtcca gacaaagagc aactatataa aaggatgatg
     1981 aagaaaatgg tgaacggttt aaaagtgaaa acaggccctc aattttattt atatgaagaa
     2041 ggtggtattt caaaagtttc tgatctcttg aaatcatacg gcgcgaaaag agtactggtc
     2101 acccacggaa cggtatcatg ggaaaaggcg ctacctaaat tagtattttt gaatgacgaa
     2161 acgattcaat ttttttatca tcgttattct ggagaatgta gttatgctga agccagacga
     2221 atagctacaa tcatcaaaaa aatgaaatcg atttcgtagt tggcgttggc ggaggaaaac
     2281 tgaccgatct agtgggttat agtgccaatc ttgcaagaat cgatttcgga gtcatcccta
     2341 ctttagctag taattgtgct ccatggacac ctcttgctgt catgtataag gagaatggac
     2401 aagcagaagg gaaaacagaa cacttttaca gacaagctgc tttttttata acagatcccc
     2461 aattagtgat tgattcccct gttccatatt tcattgccgg gttagcagat acactagcaa
     2521 aatggtatga atctgagctc atcttagaac aagatttttt gcaagatgaa tcatttttga
     2581 agctagcaca agatactgcg aagatctgca aagaagagat tttgaatcat tctgctaaag
     2641 caatccaaga tatggcacaa agaaaactga catcggagtt caaacagctt tcagagatta
     2701 tctttgctgt tgctggttta gttggaggat taggagataa atacgcaaga aatgcggcag
     2761 cacatgctat gcatgacgct atcagtaaat atattccgga aagccatcgt tttctacatg
     2821 gagaaaaagt agcgtatggg attttctatc aactggcact agaaggtcgc tgggaaaaaa
     2881 tagacgagtt atatcctttg tatcgggaat tagaactgcc ggtttctcta gaacaaatgg
     2941 gattatttcc gaaagataaa cagataatag ataaaatggc tgaatttatt gattcgaaag
     3001 agaaagtgca tttgattcct atagaggtaa cacagaaatc actgatacag tcaatgatgg
     3061 aactggaagg ttatatcaga aagatctgaa ggaggaggac acatggaaat attaagtgta
     3121 gtacaaagtt tgttgattgc tttatgggta gccgcggtta tgtcccgctg gttaggtgga
     3181 ggagccacgt tgaccttacg tttttctcct ttgatgactg gattagtcgt cgggttgatc
     3241 atgggggaag ttgcacaagc gatgattgtt acagccgctt tgcaaatgat ttatatgggg
     3301 gttttttctc caggaggatc aatgccagct gaaccatcta ttgcagcagc tatcgcagta
     3361 ccagtcgcat tattagggga tctgtcgcca gaagcagcga ttgccgttgc ggtgccagtc
     3421 ggattgctcg gaagttactt gtatcagttt cgttttttca tcaatacttt tttaggtaaa
     3481 tatacagaca aagctgtaga agatttgaat acaaaagcta tggcccgatc aattattctt
     3541 tatccaacgg ttgcctcatt tttattattc gttccgcttg ttttcattgc tttatattta
     3601 ggggcgccat tgatcgctga tgtaatcaaa gctttagagg gaacagtcgt tatccacatt
     3661 cttgaagtag taggtggagg gttagcggca atcggtatcg cgacaactgt atacgttatc
     3721 ggtcgaaaag attatctggt ctttttcttc cttgcttatt ttatgagtgt tgttttgaaa
     3781 tctttagata ttacgatggt tacttatgcc atttttggga ttattatcgc tttgatcttt
     3841 gtccagtcgc aaaaagggaa acaagtacag cctgccacaa gcaataacaa ttcagcatct
     3901 tttgatgacg aagatgacta tgatgacgga ttttaattta ggaggaatgg gaaatggctg
     3961 aaataatgga taatcaaaca ggtgctcctg aagaaatcac gaaaaaagat gtaacaaaag
     4021 cctatctgag atggcatttt gcaaacgaaa ttcctcactc ttttgaacgt tacttggcgc
     4081 catcactact atacggaatg atgccaatct tgaaaaaact ttataaagat gaagactctt
     4141 taaaagcagc atataaacgg cagttactgt tcttcaacac tcaattaagc tggggtggcg
     4201 gtgtgattac tggcctaatg tcttctatgg aacagcaaag agcagaagaa gagtatgaag
     4261 aaaaagagat cttgatgcag gatgacttga tgtataacac gaaagcggga ttgatggggg
     4321 cactagcagg aatcggagat gcaatcgatt caggaaccgt ccaatatatt tttatcgcaa
     4381 tcgcggtacc ttgggcacaa cagggaagtc ctttaggcgc actttttcct tttatctgct
     4441 ttgggttgta tcaggtttta ctaggggtat ttttcgcaag acaagctttc actatgggcc
     4501 gtaatgctac aggcctaatg caaaacactg gtgttcagac agcgattgaa atgctatcag
     4561 ttttagggtt gtttatgatg ggggtgcttg cgggaaacta tgtaaaagta gaatcttcat
     4621 tgaaatttgc gatatccgga cgtgagtttg ttatccagga aattcttgat caaatcgtgc
     4681 cagggatatt acctttagct gtcgtaatgg gtgtgtactt gttctatacg aaaaaaggat
     4741 tgaaagtcac acaagcatta ttatggctga ctggaatatt aattgtgcta gcaggaatcg
     4801 gtattttata aaaagaaggg aacgtgaata agatgggtgt agtcaattta gcgcgtgtgg
     4861 atgagcgttt gatccatggg caagtaatgg taacgttatc tcaaaaaagt ggagtgaatt
     4921 ccatttttgt tgtagacgaa gttgtagcaa aagataaatt tatgagagat ctttacaaaa
     4981 atgcaggaag ccgtacagga caaaaaacaa ttgtcattac accagagaaa gctaaattct
     5041 attgggatga atatcaattt aaagaataca attgtatttt gatcgcaaaa acagtccaaa
     5101 cgatttacga cttagtaaaa cacggaatcc ccatggaaga attgaatatt ggagggattg
     5161 ctcaaaaaaa tcctgataca gatcttttag tgacgaaatc tgtttattta aataaatcag
     5221 atgctgaaaa attgaaagaa atgaatgaac aatatggcgt aaagaatatt tattttcaag
     5281 ccactccatc tgcacctaaa acaggattaa aagatgtatt gaacaagttt gatttgtcat
     5341 aaacaaatgc ttagaaaaaa gataaagagg gcgaaaaaat gaagaagaac gagctgtttc
     5401 gtgattggga atttcgttat cgctatattt accgtaagcg ccgtacaaaa aagagtaaac
     5461 agcgttttct ttcagcatta gtatcagata tttacagtat gagaacagat gtgacagtca
     5521 ttgcttacga tacgctagcc taccgatcaa aaaatattta tgttggagat atcgaaaaag
     5581 cagagaaagt tatctgtact tattacgata cacccgttca tgcattagga agttatttca
     5641 tgtttgattg gaaagaccaa aggaaaaaaa caatctactc tatattattg tccttcattt
     5701 tgttgttcag tttaggttgg tggggaatga tgatatataa caaaaatcct catcacgtat
     5761 tcgatctgct atccgtataa acaggtatca ctgtactagc atttgggagc tactttttct
     5821 tattgggaaa agcagctcgc ggatggccta gcagacaaac ttttattcga aatacttcat
     5881 ccatattaac gatgctagaa atgatccgta cgattgatga tccgagtgta gcgtatgcgt
     5941 ttgttgatga aggctgttat ggagaaaagg gcttagacag cgtgagatcg ggtatgaaaa
     6001 aagaggctat tcttttttat ctagactctg ttggggcaga tacaccgcta cagttttcgg
     6061 gaaattattt ttcaaataaa gaacagtggc tgaaacaagt tgataagttg aaagaaaaaa
     6121 acgtcaatta tatttttagt gcaagaaaaa agcaagcgca gttcttctat ttgaccaaaa
     6181 cagatttacg agggaaaaca tttaattggc aaaatgccaa tcagattatt gctctttttc
     6241 gataagtaaa aagtgcaaaa caaggagtga gaagactaat gttggaaatc atgatcgtta
     6301 cacatggtaa gttgagcgaa ggactgaaag atgcggcgga agtaattgtc gggacatcga
     6361 gccatattga aacaatgtgc ttagagtctg gggaggacat ccaaacattc ggagagaagg
     6421 tcaaagaaaa attaactatg atcaacacag cagaacccat gctagtattt gttgatctaa
     6481 ttagcgcaag tccgtacaat caagcgctct tggcagtcaa tgaactgact gaagaaaaga
     6541 aagcagccac ttatgtcata gggggcgtga atctgccaat gttgttagaa gcaatcaatc
     6601 atcagatgct tcacacaccg atagaagaag cggtggaaca aattgtcaaa caaggaaaag
     6661 ataatttaac tgtttggcat gcttcagcgg aagaaaaaaa attagatgag gacgattttt
     6721 agaaggaagg ctgaaaagat gaagtgggga tttagatggt atggtgaaaa aggggattcg
     6781 atcccattaa aatatgttcg ccaaataccg ggaataaatg ggataattgg aactttgctg
     6841 actaaaatgc ctggagatgt ttgggagatt tcagaaattg aggagttgaa agcatctgtt
     6901 gagagccaag gactggaact gcttggaatt gaaagtgtag cgatccacga tgcaatcaag
     6961 gcagggacag atgaacgaga ttactatatt gataactata tacgaacaat caaaaacttg
     7021 tctcgatgtg gggttcgatt gatttgttat agcttcaaac caatttttgg atgggcgaaa
     7081 actgatttgt ttaatgaaaa caacgatgga agctattctc ttttatacga tcagaaaatc
     7141 gtcgatcaga tggagccagg agaaatgtat caattgatac acagccagtc aaagggattc
     7201 aaattgccgg gttgggaaga agaacggttg aaaaaattcg ctcagctgga aaaactgtat
     7261 gaaggaatga ccgaagaaga attatttgaa aatctgactt attttttaga aaaagtgatt
     7321 ccagtgtgcg aaaaagcaga tgtaaaaatg gctatccatc cagatgatcc gccatgggaa
     7381 ctttttggtt ttcctagagt gacgaaaaat cttggcgact tgaagaaaat tctaaatgca
     7441 gtagattctc catataatgg actaacgctg tgcacaggat ctttaggggc aaatccggaa
     7501 aacgatttag ttgaaatcat ccatgaggta ggttcgcgca tccattttgt ccacttcaga
     7561 aacgtggagt ttttaggaga aagaaagttc aaagaaacgg cacatccctc tacagaaggc
     7621 tcccttgata tgtatcatat tatgaaagcg ttggtgcaag aagggttcga cggtcctata
     7681 cgccctgatc atggtagaac cgtgtggggt gaagtggcta tgcctggata tggcctgtat
     7741 gatcgtgcaa tgggactcac ttacatgcaa ggtctatatg aggcgataag taaggaaaaa
     7801 ccactgggag agcattatga ataaaatcaa gctcttcatc tttgatatgg atggcctttt
     7861 attagaaact ggacgtttag cttatcgtgc ttatgtaaaa tcagcacaaa agtatgatta
     7921 tgaaatgcgt aaggaagtct attatttact aactggccaa acagaaatgg cgataagaga
     7981 acaaatgggc attttgtacg gagaagatgt tccatatatc aaatggcgag aagcaatcaa
     8041 tcaatataaa gaaaaaatcg tgaaagaaga taagagagta tatacgaaaa aaggagcaga
     8101 agaaatttta tcttttgcaa aagaaagagg aatccataca atcgtggctt cgtctaatac
     8161 acgggtaaaa gtagaaatgt atttaaaaat ggaaaatcta tatgccttgt ttgatcaaat
     8221 catttcaggg gatgatgtaa aaaaaggaaa gccggaacca gagatctttt tgaaagcttg
     8281 ttcaaaaatg aatattcctc caagtgaagc acttgtattt gaagactcga ttgcagggat
     8341 cgaagcagca aggcgagcgg gcattttgtc ttttctcatc aaagatcatt tgatagggtt
     8401 gcccgatcat aatgggaagc ataagttgaa agtagatgta agtttatatg aatccaataa
     8461 agcagactat gaattttatg acctgcatca agcaaaacga tttttagaaa gtaaagggtt
     8521 gtatctctaa taaagaggac tattttctag tcctctttat ttattgtcct gcacgactga
     8581 gggaacagat gaaaaagaag taatttttta ttttgaagta tctaccttat ttgtagtgaa
     8641 caatttagaa caaacacaaa acgcatctac tgttatctac cagtagatgc gtttaaagag
     8701 aagaacgaga atcgtccata tgagggtttc cacggctcta taatttctag gactgataga
     8761 aaatcattct atcggtccct tttgagcatg aaaaaacaaa acatacagaa gctccgttag
     8821 aattgaatca ccacaacaca atcaaaggag cgttctgtat gcctgatctt catcatatca
     8881 aactactact tggaatcaaa gataaaaaca ttttcatcac aaatgtggaa tcgaagagca
     8941 ttaagaatat caagagtctt gttgtttctg cgacgttaag taaagaaatt cgtcgctgtc
     9001 ctttgtgtaa acagatgaat catgaaggca tgatcgttaa gaacggaaag aaaaaatcgc
     9061 tcattcaact taataaatgt gcgaaccaac tgacttatct tgctttagcg aaacaacgct
     9121 accactgtcg tggctgccac acctatttta cggcgaatac ctacattgtc gatagaaact
     9181 gctttatcgc taaacaagtg cgctataaaa ttttagaaga gctaactgaa aaacaagcaa
     9241 tgacgacaat tgctaagcat tgcggcgttt cttggtcgac cgtttctcgg acactcgctt
     9301 ctctgatccc catgacaaaa gtaaaaagaa attggttacc gcgttgttta ttaatggatg
     9361 aatttcgttc actgaaaaat caagtcggtc cctactcctt ttcttgtatg gatggcgata
     9421 ctggaaaatt actggatatt cttccttcac ggaagaaaaa ggctctcgtt tcttatttta
     9481 tgcaatttga acgacgcgca cgattgaagg taaaggtcat cgtgacggac atgaatgttt
     9541 cttatgcctc tctgataaag gaatgttttc caaaggcaaa actcgttgtg gatcgttttc
     9601 acattgtcaa acatctcatt cgtaaatttg aggatattcg cgtacgaatc atgaaaagct
     9661 ttgatcgaaa cgatccaaca caagccaaat attatcgaca attaaaggcg ttgtctcggc
     9721 ttctgatcaa acgacaggac aagcttgttt atgacaaatg gaccaaatgg cgcaattttg
     9781 gttgggcgta tctcaccgaa agtgaagtgg tggatcgtct gctttcgatc tctgacgaat
     9841 tacgaatcgc ctatttttac tatcaagaaa ttcttcaagc gtttcacgac aaagaggcgg
     9901 acactttctt taaacttgta agaacgatgc ccaattccgt tccaaaagaa ctgcattcta
     9961 taaaaaaagc ttttataaac tatgaatccg gcattcgttt agccctcgaa ttgccttatt
    10021 caaatgccaa aatagaaaat ctgcataccc acatcaaagc attgaaacga gtcgcttatg
    10081 gctttcgaag ctttcggaaa atgaagacca gaattttctt actaaataat ctgattacct
    10141 atgaatcaaa aaacatctaa agtagaaaag gaattctacc ttagatgttc ttatctcact
    10201 ctatcagtcc tatttgacaa agagccgttt ccactcctta tgcagatttt tttaattctt
    10261 gcactgcttg ttcgatcgta acacctgtcg ctacatgatt ggtattttct ttgtcaaaaa
    10321 cgacaaatag attcaaatct gtatccaagg ttactcgata ttgtatatct ttgttataga
    10381 atgtcatagc cattctcctt tctttaaata cttgctgaaa ggcaacgaac gcgttgataa
    10441 cgaaattcca tttataagta taccaccaat tgaaaggttt tcatagttaa gaaattttaa
    10501 agtcaaaagt tgcagataaa agcaaataaa caagaaaaca aaaagtgaat caatcgcggt
    10561 tattcaactt tttcttcgat tcgaagacaa tagaag
//
LOCUS       DAKBVG010000091        10453 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000091 DAKBVG010000000
VERSION     DAKBVG010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.091"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            85..363
                     /locus_tag="OUD09_001607"
     CDS             85..363
                     /locus_tag="OUD09_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9692880.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            382..1590
                     /locus_tag="OUD09_001608"
     CDS             382..1590
                     /locus_tag="OUD09_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCT9692881.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(1738..2103)
                     /locus_tag="OUD09_001609"
     CDS             complement(1738..2103)
                     /locus_tag="OUD09_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HCT9692882.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(2250..3023)
                     /locus_tag="OUD09_001610"
     CDS             complement(2250..3023)
                     /locus_tag="OUD09_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCT9692883.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(3197..4057)
                     /locus_tag="OUD09_001611"
     CDS             complement(3197..4057)
                     /locus_tag="OUD09_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HCT9692884.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(4057..5475)
                     /locus_tag="OUD09_001612"
     CDS             complement(4057..5475)
                     /locus_tag="OUD09_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCT9692885.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSISTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(5532..5981)
                     /locus_tag="OUD09_001613"
     CDS             complement(5532..5981)
                     /locus_tag="OUD09_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCT9692886.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(6426..6689)
                     /locus_tag="OUD09_001614"
     CDS             complement(6426..6689)
                     /locus_tag="OUD09_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HCT9692887.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(6788..8083)
                     /gene="rho"
                     /locus_tag="OUD09_001615"
     CDS             complement(6788..8083)
                     /gene="rho"
                     /locus_tag="OUD09_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCT9692888.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(8080..9351)
                     /locus_tag="OUD09_001616"
     CDS             complement(8080..9351)
                     /locus_tag="OUD09_001616"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCT9692889.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(9516..10385)
                     /locus_tag="OUD09_001617"
     CDS             complement(9516..10385)
                     /locus_tag="OUD09_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HCT9692890.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
BASE COUNT         2905 a         2219 c         1838 g         3491 t
ORIGIN      
        1 tttccatcac ttatgcgatt tttttccttt tttttattcg ttatactgca gaagtaaaat
       61 tttttaagaa tagagggaaa aaacatgaat tactggatga acacaatcat taatcgatta
      121 gaaacagcat atcaaacaag atttgatatg aaagcttctt tagtcttttt aaatgatgcg
      181 tatcaaaact cgatcgaatt gatcaaagca gtggatgaga atccaacaaa cgaatgcgaa
      241 gaatttttaa atctgtttat gtctacaaga gatttattta ttcgccaact tgttgatcgc
      301 tatccatcca attatcacga tgtagaagtt caaatacaaa aattaaaagc atattcagct
      361 taaggggaat gtataacata catggaaaag aaattattta ataaatattt tattggaatc
      421 acattgatca attttattgt ctacctgatt tattatctgt tgatggtcat catggcagtc
      481 attgcgcaag acaccttaca cgccagcttg agccaagcag gttttgcttc tgggatctat
      541 atcatcggaa cattgctggc acgcttattc atggggaaac agttagaact atttggtcgg
      601 aagtttacct taagaggcgg cgcgatcttc tatctccttt caacattagc atatctttat
      661 acgccaacta tcggggcatt gtatttagtt cgattcttga atggattcgg ctacggtaca
      721 gtttctacag ctacaaatgc tatcgttact gcatatatcc ctgcatctaa aaaaggggaa
      781 ggcatcaatt attatggatt aagtactagt ttagctgccg ctgttgggcc atttttagga
      841 atgattttgc tgaatatgac tgattttcgt ttcattatct ggttttcaat tgtactggtc
      901 ttctttgtta cgattgcctg ccttgtcttt cctgtaaaaa atattccatt atctgaagaa
      961 caaaaaatat ctcttcgctc atggaatatc aatagtttta ttgaaaagaa agcacttttc
     1021 attacaggaa ttgccttttt gatgggtctt tcttattcaa gtgttttatc atttttatct
     1081 tcttatacaa aagtaatcca cttagttgct gctgggtcat ttttcttcgt cgtatatgct
     1141 ttggtcatca catttacacg tccattgact ggaaggattt tcgatgtcaa aggagaacaa
     1201 tacgtgatgt atcctagcta tttattctta acagcaggat tatttttact gagtgtaacg
     1261 acgaatagtg tgacattgct tatctctgga gcattagtcg gcctaggtta tggtacgttt
     1321 atgtcaaatg gacaggctgt ttgtttaaaa atcgtccgcg aacatcgaat cagtattgct
     1381 ttatctacct actttattgg tttagaccta ggcttaggcg ttggtcctta tttgatgggg
     1441 atgctaaaat ctcttacttc atttagagga ttgtatgtga ttgccggagt cattccattg
     1501 atatgtacag cactctatct tgttaatggc cgaaaacata gtcatgaagc agagggcgaa
     1561 ttgattgaat cattgaaaaa tgaaatttga tcttgatgaa tagataaaaa aaaccgttca
     1621 gatctgaaaa taatctgagc ggttttttga gtaaaaatag tttatgatta ttcttaatta
     1681 aacatctatt tatttttaaa gctttcattt atcgataaga atagtttaac tgttgattta
     1741 ttccattttc cgttcatctg gtacgatgcc ttgacttagc aaccacttgt ggaagaacca
     1801 ttctgccaca ccgatcaata gagcaggaat caatactttc atccaaatat cgactgtatc
     1861 attcataaaa tatagataga gccacaagat aactgcagct gaaccagcat ctgcgatcgt
     1921 cgctgcgatg ttccctactt tacgtaagat caacagatcc cctagcacat agatcatgac
     1981 tacaataatt accccaacaa tcgttgaatc cataaagcta acaccgtata aacctgtcag
     2041 cacgacccac agaatcgccc agatcatgat tgccttcact accaacgcct tcacatgttt
     2101 cattttctgc actcctttct cctggataaa aaagtaagat tatctaacaa gataaccttt
     2161 tcattattat accatcggct gagagaagcg agcaaagata aggtcctatc taaaaatgag
     2221 gaagccactt agaacattca cgtaataatt tatccgtaca gacattcgat atggtgttcc
     2281 tttactttcg catactgttc ttttgtcagt tctttacttg tcaccagata atcgatttca
     2341 tgaaaagcaa tggcttgatg catgaattta tgtgcaattt tttgctgtgt aactaaaacg
     2401 gcaagccggt ctgattgttt tctaaaagct tttttgacca ttgcctctcc ttcatgagtt
     2461 tctgttactc cttggctgaa atctagcccg cgacaactca ttatcgctaa atccgcataa
     2521 tatgacaatg catcgttata ggcttttgaa ccgttgattg ttgctcgttt cggaacaatt
     2581 gcccctccca atacagatac ttttgccgtt gtatattcac tcaacatagc agctgtgacg
     2641 atgccattcg taatgatctg caaaccatca aatcttgcta ggtttctgac taagtgtaaa
     2701 gaggttgtac tggaatccaa gaataagacc atatcatctc gaatcaaaga agaagctgta
     2761 tctgcaatct tacgtttttc tgtttcttct tctaatttgg tgatcttcat caggtaaggc
     2821 acgtcaattt ttcgattgtc aataagcatg gcgccaccgc gaactctacg gatcaatcca
     2881 cttttttcca gaaaaacaag gtcacgttta atcgtggctt cgctataatt tactaactcg
     2941 atcaaatcgg tgactcttgc aaattggttg ttttttagat aattcacaat ctcctgctgt
     3001 ctattctcca cttcattcaa cataaaatca ccccatttac tttatatagt aacaggaaaa
     3061 aagaggttgt gacagaagtg attggcttcg agaaataaga agaaattttt cgaaaaaact
     3121 acttctgaac atcgtgtgtg gttaagaggg tatgacaaga ctattgtcat accctcttga
     3181 ccacacattt ttatttttag tataaactag ccttgcctac tgtgttcaaa atttccattt
     3241 tatgtttgac cacttcttgt acctttttat tagctgctgg ataaattgca ttcggctcgt
     3301 acatcgatgg attttcactc aatatttctc tcaaagcatt gaaaaagacg cttttaagat
     3361 ctgagctcaa attcactttg gcaactccgt attttactgc ttcgctgact tctgcgtccg
     3421 gattacctga accgccatgt aaaacaaatg gaagatcgat ttgttggttc aattctttca
     3481 gtaagtcgat atttaatttt ggagttctgt ttttcggata caagccatgc gctgttccaa
     3541 ttgctacagc aagtgtatcg attcctgttt ctttcacaaa aatcttcgct tgatcaggat
     3601 ctgtataaat gattgtttca gcacctcctt cagcagaacc attattccca atcgttccaa
     3661 gctcagcctc cactgagaca tttaccgcat gagctaacgc aaccactttt ttcgtaatct
     3721 gtacattgtc gtcgaatgat ttcaaagacg catcgatcat cacggaagaa tagccgtttc
     3781 gaatcgcccg taagacatct tctatcgtac cgccatgatc caagtggata acgatcggca
     3841 gtttacttgt ataagcatag gctcttactg ccgcaacaaa ctcatcactt aaataggcga
     3901 tttcatcagg atgaatttct aagattactg gtgcttctaa tctctctgct tcttcgatga
     3961 ttgctttcat catatcatag ctgcaaatat tgaaggctgg tactgcaaat tgattctctt
     4021 tagccacttt caataactct ttcattgtat ataacattac gcttccacct catttgttag
     4081 attttcattt ttcgtccact tgtcttgttt tttctctttc actgtttttt tcaatagtcc
     4141 aagcataaag gctgtagtga ctgtacctga tgcaattgcc aagacataca taggtaaatt
     4201 gttcataaca tttggaatta gcatcaccca aatccctcca tgaggtgcca ttgaagtaac
     4261 tcctactgcc atggaaatcc ctgcagcgac tgctgaacct gtcatcaaac taggtatcac
     4321 gcggatcggg tcagataccg caaacggtat agcaccttct gtaatatacg aagcgcctaa
     4381 aatccagcat gattgacctg aagtccgttc ttctttggta aatttatgtt tgaataataa
     4441 agtggctaaa gcaataccta ggggaggtgt cattcctgca gccatacaag cagcaatcgg
     4501 tgcatttact ccagctgaca tcaaaccgat cgagaaagtc gaaatcgatt tattgatcgg
     4561 cccacccata tcagctgcca tcatgatccc gataagtaat ccgaacaata gcccggatgt
     4621 atttcccaag ctattcagcc aatttgtcaa agcatccaac acccatgtga tcggttgttc
     4681 aatcacaaat accatcaaaa gcccaataat taagatcgat aaaaagggga cgacaatcag
     4741 ctgataaata gcagaaaatt ctttttttac gctaatttta tttgccagaa aatcacagat
     4801 ataccctgct agcacgccac caatcattcc gccaagaaaa cctgaaccac ctgtagaggc
     4861 cagtaatccg gcaacaattc ctggggcaaa acctgctttt ccagcaatcg atgtagcaat
     4921 ccccgcgcct aacgcaggaa ccatcatggc aaacgctgta tctcctccga tccttgaaaa
     4981 tgcagctgct aacacattat agtctgctga gttgggatca gccgctgtaa tcccaaaggc
     5041 aaaactcagt gcgatgatga ttccgcctgc gatcacaaat ggaagcatgt aattcacacc
     5101 attcatgaaa tgattataga ttgtcggctt tttctcattt gcagctggtt ctttttcatt
     5161 agacacaacg aatacgccaa tatctctcaa tgcttcgtct attactactt gcggttcttt
     5221 gattgctcgg cttgtagaga cacgctttac ttttttcccg gcaaatcggt ccaattcgat
     5281 ttctttatct actgcaagaa tgattgcatc tgcctctttg atttcttgtt cagataactg
     5341 attttctgtt tttacacctt gtgtttcgaa ttttgcttga tggtttagat ttcttgctgt
     5401 tacttctaat ttttctgccg acatataagt atgagcaatt ccagtcgggc aagcagttac
     5461 accaacgatt ttcatgatta ttttcctcct ttttcttcac tatcgatact cggacaaaga
     5521 ctctactatt ttcattcttg actaatctgt ctgatgatat ctgctggcgt agtggcttct
     5581 tttattccct ttctaaaatc tgcattcatc aatcgtctgg agagtgtgct gagaatctct
     5641 agatgcatat ctgctgattt ttcaggaact acgatggcaa aaatatattc aattggcatt
     5701 tcatctatcc cgctatagtc cgttaatttg gctgtatgaa aaaaacacag tgctggtttt
     5761 gtcacatatt tgctttttgc atggggaatc ccaaatccat cttctattcc tgtggagatc
     5821 tcctcctctc gtttgaatag tgccttaacg actttttgaa catttcttac cttatttgtt
     5881 tttttcaaca catcccctat tcgctcaaat aatgatcctt ttgaatctat cgatggatcg
     5941 aaaacaatca gttcttctgc gatcaattga tcaaacctca taaaaaacct ctcccccaca
     6001 aaaaatgtta acgttttctt ggaactgagg tcagtatagc atcgctagct gtaccgttca
     6061 atattatgaa tgaacagagg aaaaagaccc atcatccgcg tgtataagtc attaaaatga
     6121 acgatctagc tcacaaacaa tcgcaagtct ataacaaaac aaaaagcctt ccttgaaagg
     6181 aaagcttagc ttctaatttt aggtaaatgg ttttttactt cgaaataacc cctcttcatt
     6241 tcgttaatat aaggtgtttt tatcaagtgg ataattaaaa ctatatacta gtttttcatt
     6301 gttttgtgca ttatgatcct acgacttttt cagcatcaaa cgaagtttta aattggcaaa
     6361 aaacataaaa aaacagacta tccctatcag atagtctgct aaacgttgag ttatcagcgt
     6421 ttttattatt ccgcagcgtt tgcgtctttg agaccgtatt ttttgttgaa acggtccaca
     6481 cgtccgtctg cttgtgtgaa tttttgacgt ccagtgtaga atggatgtga gtcagaagtt
     6541 acttcgacac ggatgactgg gtatgtatta ccgtcttccc attcaactgt ttcttgtgaa
     6601 gtttttgttg aacctgacaa gaatttgaaa ccagttgttg aatccatgaa tactactggg
     6661 tggtaatctg gatggatgtt ttctttcatg attttttcgc tcctttgccc tgctccattt
     6721 gctgaaccag agttgatttg tcgatttaca acattaacat cttaacatgt ttcagtcaag
     6781 gttgcaatta tcttttcaga tttctggtcg gatttttttt accaaaggag acatcttgaa
     6841 attctttgaa aaattgctga ttgttttttg ttttacgcaa aaagcggatc agttgttccg
     6901 tatattctaa tgaatctcca gtcatatttt ttcgaagttt ccaaatttct tctaattctt
     6961 cacttggcat caataattct tcttttcggg tacctgattt tttgatatcg atggctggga
     7021 agatccgacg ttcagataat tcacgtgaaa gttgtaattc tagatttcca gttcctttga
     7081 attcttcata gatcacgtca tccatacggc ttcctgtatc gactaaagcg gtcgctaaaa
     7141 tcgtcaaact gccaccttct tcgatgtttc ttgctgcccc gaaaaatttc ttaggtttat
     7201 acaatgcggc tgggtcaatc ccgccactca atgtacgtcc gcttggtgga acgactaagt
     7261 tataggcccg agcaagtcta gtaatactat ccatcaagat aacgacatct cgtttatctt
     7321 cgactaaacg cattgcgcgt tccaatacta attcagaaac tcgagtatgg ttttgcggct
     7381 gcagatcaaa tgtagaagat accacgtcgc ctttcacgct tcgttctaga tccgttactt
     7441 cttccggtcg ttcatcaatc agtagaacga ttaattctac ttccggatag ttttcggtga
     7501 taccatttgc gatttctttt aatacagaag ttttccctgc ttttggcggt gcgacgatca
     7561 atccacgctg accaaaaccg attggagaaa agatatcgat cattcgggta gatagtcttt
     7621 gcggtgttgt ttccagttga agctgtcttt gcggataaag aggtgttaag gctggaaaat
     7681 gcggacgttc ttttgcttct tccggatctt ttccattaac gctttccacg tgcatcaaac
     7741 cataataacg ttccgattct ttaggtggac gggcttttcc tgccactttg tccccatttc
     7801 tcaaactgaa tcgtctgatt tgtgaggcag aaatatagat atcttctgca cttggtccat
     7861 aattgattgg ccgtaaaaaa ccgtaaccat cttgaccaac gatatccaat attccttcca
     7921 tataataaaa tccttgtttc tctgcttgtg cacgaatcac agctagagat aattcctttt
     7981 tattcatctt actgtaataa ggaattttaa attctttggc ataactataa acttctttta
     8041 acgttttgtt ctctagttct gccattgtca aataatcgct cattcagctc ccacttcctc
     8101 tatcatttca attgatgccc ctaaggctgt tagtttctcg ataatatgat cataaccgcg
     8161 taagatataa tctacattgt aaatcgtcgt tgtcccttct gccaatagtc cagcaatcac
     8221 taaacaagca cctgcccgaa gatcagaagc cacgacttct gtgccgtgaa gtttatttgg
     8281 accattcacg atgatcatat ttccttccac ggtaatatca gcgcccattc gtgctagttc
     8341 cggcacatgg ttcacacgtt tcgcatagat cgtatcgatg atttctgaag tgcctgtagt
     8401 catcaatagt aacgcagtaa gtggctgctg cagatctgtg gcaaatcctg gataaggata
     8461 ggtcatgatg tttgcgggtt ttaattcttt cgaggggtaa acttcgattt catcttcgct
     8521 gattttcata tggacaccaa tttcctgcaa ttttgctata aagctttcca aatgttcaaa
     8581 gatcacattt ttcactttga taccatttcc tactgcagct gctaatgcta gataggttcc
     8641 tgcttcgatg cggtcaggaa tcatgaaatg ccgacagcca tgtaatgttt cgactccttc
     8701 gatacggatc acatctgtac ccgctcctcg aacttttgcc cccatattat tcagtaaagt
     8761 tgccacatct atgatttccg gttctcgtgc agcgttttca attactgtct gtccttttgc
     8821 ttttacagca gctaacatca cattgatcgt tgctccgact gaaaccacgt ccatgaaaat
     8881 ccgattgcca cgcaatcctt tattttctgt ccgtaaatac atggcaccat gttcatttgt
     8941 cacttcagca cctagagctt caaatccttt gacgtgtagg tcaatcggtc tagggcctaa
     9001 ataacagcca cctggcaggc ctactaccgc ttcgccaaat ttaccaagca gtgttcccat
     9061 aaaataataa gaagcacgca aactattgat ttttccgtgc ggcatcggaa tcgatacgac
     9121 ttctcgagga tcgatttcta gtatgttgtt ggaaaaatgg acagttgcgc ccatgatctc
     9181 taaaatttca attaatgaat gaacatcttg tatgtccggc acaccttcca agatcactgg
     9241 ggaatcagct aagattgcag ctggaatcaa tgcgactgca ctattttttg caccgctgat
     9301 cgttacttca ccagctaaag gtcggtttcc tttgattacg atttttttca tctgtttcca
     9361 ccttgtttat taatcttttt tcatggatat gcttgtattt tatcatagat aaaaaaaaga
     9421 aacgagcatg gtctgccgtt ttaagtaaaa aagaatggag agcgacttca aaagtcgttc
     9481 tccattccta tatatcgtat ctgttatgtg gatgcttacg ctttgttagc tgaaccgaac
     9541 cagtcgatat gagatttagc atcttcaacg attgctgctt tacctggagc taataattta
     9601 cgagggtcga atcctttacc ttctttatct ttaccagctt cgatatattt acgagtagct
     9661 gcagcaaatg ataattggaa ttcagtgtta acatttactt ttgagatacc cattgagata
     9721 gctttttcga tttgatcttg aggaatacct gatccgccat gtaatactaa aggcatgtct
     9781 ggacctacag catcagcaat tgcttgtaag tggtcaaaag ctaatccttt ccagttttct
     9841 ggatattgac catggatgtt accgataccg caagctaagt agtcgatacc tgtagcaacc
     9901 atttgtttgc attcttcaat atcagctaat tcgccgttac cgatgattcc gtcttcttct
     9961 ccgccgattg aaccaacttc acattcaact gagatacctt ttgcatgtgc tttttcaaca
    10021 acgtctttag ctaatttcaa gttttcttca aatggtagat gtgaaccatc gaacattact
    10081 gaagtatagc ctaattcgat acattccaaa gcagcttcgt aatcaccatg gtctaagtgg
    10141 atagcaactg gaacagtgat gttcattgct tcaactaaat cagtgattaa atctttacag
    10201 attttgtaac cacccatgta tttagctgca cccattgatg tttggataag tactggtgct
    10261 tgttttgctt cagccgcttc aaggattgct tgagtccatt ccaagttgtt tgtgttgaat
    10321 ccgccgacag cgtaaccgcc tttacgagca gcttttaaaa attcagctcc tgatactact
    10381 ggcataataa aattcctcct aagttttgta aaactaaatt tgttaagaca attcttaacc
    10441 aacgacccta ttt
//
LOCUS       DAKBVG010000092        10434 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000092 DAKBVG010000000
VERSION     DAKBVG010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.092"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(163..648)
                     /locus_tag="OUD09_001618"
     CDS             complement(163..648)
                     /locus_tag="OUD09_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HCT9692891.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    738..1083
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1128..1769
                     /locus_tag="OUD09_001619"
     CDS             1128..1769
                     /locus_tag="OUD09_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCT9692892.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            1788..2423
                     /locus_tag="OUD09_001620"
     CDS             1788..2423
                     /locus_tag="OUD09_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCT9692893.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2441..3256
                     /locus_tag="OUD09_001621"
     CDS             2441..3256
                     /locus_tag="OUD09_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HCT9692894.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3575..5011
                     /locus_tag="OUD09_001622"
     CDS             3575..5011
                     /locus_tag="OUD09_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCT9692895.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5200..7647
                     /locus_tag="OUD09_001623"
     CDS             5200..7647
                     /locus_tag="OUD09_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCT9692896.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            7877..8944
                     /gene="rlmN"
                     /locus_tag="OUD09_001624"
     CDS             7877..8944
                     /gene="rlmN"
                     /locus_tag="OUD09_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HCT9692897.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(9137..10240)
                     /locus_tag="OUD09_001625"
     CDS             complement(9137..10240)
                     /locus_tag="OUD09_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HCT9692898.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
BASE COUNT         3448 a         1787 c         2276 g         2923 t
ORIGIN      
        1 aataaatatg gatttggcta actatggaag aatactcgta ttagttttca gcgttcatac
       61 tagttgttac attgtttgat tggccaaaag aatttctgat atattttgtg ctaaataaag
      121 aaaaagacgc ttgaattggg aaataagaaa actactctat cattacaaat tttcataaac
      181 tggcagatgt tctaagtaaa caagtatctt ttcaaagtcg gaaaaatcca taatcaacgt
      241 agtggtattg atatttggat gaaagcctac ggtatcttga cgatcaatag cagcatcgat
      301 cacgacttgg atttttttct ctttgtcgtg catcaatgct aatggagtca ctgtacctgc
      361 tggaacgcct aggaggtcag gaagctgttc ttcagaaaca aaagataagc gcttttcttt
      421 taattgttca gctagttgtt ttaaatcggc gtgcttttca tcaggcaaga tgacaagata
      481 gatttgtttt ccttttcttg atttcaataa tagatttttt acttgcggac caggtaggga
      541 aaaagggaca tttttgactg acgtaatcgc aggatgatcg actttttgaa aagagatacc
      601 cagttttttt agtagttcat aagcttcttg ttcggttgca cgactcatcg cgcatcaccc
      661 tccttttttt attagtgtat cataaaaaag agttgacaaa tgaaaaataa tcatgtaaat
      721 tacacgtaaa gttaaaacaa atgacatcga aaagaagagt agccgttagg tgtttttaga
      781 gagtctctgg ttggtggaaa gaggcagcag ttaaccgtga aacacatctt ggagtcgatt
      841 ttctgaagcg tatagtagga aaatccgggg agcccgttac agctgcggtc gttgctagat
      901 gaccagataa ggctggcttt tccgtgagga gtcggtaaaa aaaggtggaa ccacgtgatt
      961 aacgtccttt gatcttcgga tcaaaggact ttttgttttc atctgaacgg caacgactgt
     1021 gtgaggtttt ttctgcaagg aaaaaataaa cagaggtgga accacgtata atacgtcctc
     1081 ttggcatttg ccaagaggat ttttttattt taaaggagga aaagaagatg gagtatttga
     1141 ttgaagttat gccgcaatta ttagtaggag cactcacaac attgaaatta tttttattta
     1201 ccttgattgg ctcattacca ttagggattg ttttagcatt aggattgtct ttgcatttta
     1261 agccgctacg ctacttgctt caaacatatg tatgggttat gcggggaaca ccgctgttgt
     1321 tgcaactgat ttttattttt tatgggttgc cgacgattgg gatcgttttt gatcgtttcg
     1381 aagcagcact cattgccttt atcttaaact atgcagctta ttttgcagag atctttcgtg
     1441 gagggataca atcgattcct caaggtcaat atgaagcagc gcaagttttg caattaagtc
     1501 cctttcaaac ggtgacgaaa atcgtcatcc cacaagtgac aaagattgtt ttaccttctg
     1561 tagggaatga agttatcaac ttggtcaaag attcttcctt gatctatata ttaggtttag
     1621 gtgacgtaat gcgtgctggg aaaatcgcga tggaacgcga cgcgacttta ctgcctttgc
     1681 taggcgtggc aatcatctat cttacattga ctggtattct aacaatcgta atgaaacaag
     1741 ttgaaggaaa attaaattat taccgctaac tgataggagg aagaaatatg attgaattga
     1801 agaaagtgaa caaagcattt ggtgaaaaaa aaataatcaa agatttgaat ctaatcattc
     1861 cagatgggaa aacattagct atcgtaggtc cttccggtgg cggtaaaacc acgcttttac
     1921 gtatcttggc tggcttagaa agttctgaca gcggagaatt ttatttagac ggtgtaccgt
     1981 tcaacccatt cgagcttaaa atgaaagaac aagttgtagg agtcgtattc caagacttcc
     2041 agctatttcc acatctgaat atttttgaaa acattacctt agcaccaaaa atggtattga
     2101 aacagacaaa agaagaatac accaaaaaag cagaagaatt agcggcttca ttagggatta
     2161 gtgatttatt gaatatctat ccttatcagc tttctggcgg acaaaaacag cgggtagcga
     2221 ttgccagagc attggctatg aatccacggg tattggctta tgatgaacca acaagtgctt
     2281 tagatcctga attacgccag caagtcgagc gtttgatcct atcattgaaa gaagatgggg
     2341 taacccaaat tattgtcacc catgatttag tatttgcaga aaatgtcggt gatcaattac
     2401 taaaagtttc cccaattcat taagaaagga gaagatagag atgaaaaaaa ccagttttct
     2461 attcgctttg atcgcaggtc ttcttctttt tgcaggctgt tcgaacaaaa aaacttcagc
     2521 agataattgg gcgactatca acgaagagaa aaaaatcgtt ataggtttag acgatacatt
     2581 tgttcctatg ggatttagag ataaagacgg gaagctcaca ggatttgata ttgatttagc
     2641 caaagcagtc tttgaacaat atgggattac ggtagacttt cagcccattg actggtcaat
     2701 gaaggaattt gaactgaaca atggaacgat cgatttgata tggaacggct attccaaaac
     2761 gccggcaaga gaaaagaaag tgcaatttac aaaaccttat atggaaaacg atcaagtgct
     2821 gatcacacct aaagaatcag gaatcacaaa ctttggacaa atgaataata agagattagg
     2881 tgcgcaaaat gggtcatctg gttatgatgt atttacgaaa caaccagaag tattaaaaga
     2941 gatagtcaaa aaccaagatg ctgttttgta tgatagtttt aatgaagcat tgatcgatct
     3001 gaaaagcaga cgggtcgatg gtctgctgat ggataaagta tacgcagact attatttgaa
     3061 acaaagaaac gaactagaag acttcaacat aacaaaaggt tcttatgaga gtgaggattt
     3121 tgcagttggt gcacgcaagt cagatagtac attagtgaaa aaaatcaatg aagccctaga
     3181 tcaactagag aaaacaggtg aattccaaaa aatatccgat aaatggtttg gcgaagatgt
     3241 tacgccgaga aattaacaaa taggggactg tgacaaaaga cgtgtcacag tccctttttc
     3301 cgaataaaca gtgttctcct gccaactcct tgtgactcgg agctgaatgg caggctcaca
     3361 acctcttggt aaacggtgtt caaaagccag tttttcggta ttaagccaaa tttatgcaaa
     3421 aatgtagaaa gccattttca caaaattttt cttactactc gaaactaagc gactttttca
     3481 caacctcctt tacatatttt tattttgttg tggaaggggt ttcgtgttat tctaaaagag
     3541 gaacgattga aggttatagg aaaggaagag atgaatggaa cacaaagaac ttacctcatt
     3601 tccaaaagat tttctgtggg gctctgcttc tgcagcgtat caagtagaag gagcttggca
     3661 agaagatggg aaaggagaat cagtctggga tcgatttgta cggatacctg gaaagacatt
     3721 taaaggaacg aacggtgatt tggcagtaga ccactatcat cgttataaag aagatattgc
     3781 tttaatgaaa gaacaaggtc tgaaagctta ccgcttctct gtagcttgga cacggatttt
     3841 tcctaatgga cgaggagaag tcaatcaagc tgggctcgct ttttatgaga gattgatcga
     3901 cgaattgatt gaaaacgata ttgaacctat gttgacttta tatcattggg atcttccaca
     3961 agcattgcaa gatgaatata atggttggga atcacgtcag atcattgaag atttcactca
     4021 ttatgcagaa acattatttg aggcattccg cgggaaagtc cattactgga ttagtttgaa
     4081 tgaacaaaat atcttcactt cattaggtta tttgttagca gcacatccgc caggtgtgac
     4141 agacccgaaa cgaatgtatg aagttaacca tattgctaat ttagcaaatg catctgtcat
     4201 caataaattt cacgagttag aaatgcctgg aaaaatcgga ccaagctttg cgtatacgcc
     4261 aaattatcca attgacagta atcctgaaaa tgttctggct gctgaaaatg cagaagacct
     4321 gatggccaat tattggatgg atgtctatat gtggggaaaa tatccgattg ctgccatgag
     4381 gttcttagaa gaaaaaggat gggcaccaac gattgaagct ggcgatgcag aattgctaga
     4441 atctgctaaa ccagattttc ttgggatcaa ttactatcaa acggcgacga atgcattcaa
     4501 tccattaaac ggagttggtg ctggaaaaat gaatacgaca ggaaaaaaag gttcatcaga
     4561 agaaactgga gttcctggaa tgtataaaaa agttgagaat ccatttgtcg aacgaacaaa
     4621 ttgggactgg gagattgacc cagaaggatt aaggatcgga ctgcgtcgta tcacaagccg
     4681 ttaccgcatg ccggtactga ttacggaaaa tggcttagga gagtatgaca agttgacaga
     4741 agataaacaa atccatgacg actatcgaat caattactta caaagccacg tcaaagccat
     4801 caaagaagca atttcggatg gagcagaagt cctaggctat tgcacctggt cttatacaga
     4861 tttgcttagc tggctgaacg gttatcaaaa gcgatatgga tttgtatatg ttgatcaaga
     4921 cgaaacacaa gaaggatctt tggaacgata taagaaagac agttattact ggtaccaaaa
     4981 actgattgaa gaaaatggag agaaccttta gttcacatac ttaaaacaag aaggtacggc
     5041 tggatatggc cgtgcctttt cttgataaaa tcatttagaa attaagaaaa actgtaaaat
     5101 aagctgaaaa atgaaagaaa gcgtcagttc tttcaaacaa aactttagtt cccttacttt
     5161 tttttgtata atgaaacttg atgaaatgag ggataataaa tggcacaaaa gaaacggcct
     5221 actaaaaaaa ggaaaaagac gaaaaaaacg aaaaagcaaa aacagcagca ggaacaaatg
     5281 ctgacgattg gtgttgggtt tgccttgatt ttatttgctg tcttcggctt tttaaaatta
     5341 ggttttttag gaatattgat tgcaaacggc tttcggatca ttgcaggaaa tacttaccaa
     5401 atcctctgtc ttttattagc cgtattagga ttttggattg tcattaagaa tacagagttt
     5461 tcaattggta aaaaccgacg ctggtttggc ggcatactct tttatttcgg tatcttatta
     5521 ttgttacatg cccatttatt tggcaaactc catactgggg aaccaaatat catggggacg
     5581 acatgggatt tgttagccag tgatataaaa caaagtcaag tagacaataa cgttggtggt
     5641 gggatgattg gagcaatcct ttatcatttt acttacttct tgattgcaca gcctggaagt
     5701 tatttagcag cagtgctatt actggcagga ggagcattcc tgttcagtaa tctggaaggc
     5761 tatcagctgt taaatggtat ccaatcaatt ggagaacgag tacaggaatt attggaaggt
     5821 gatcctgcca agcaagctcg aaagcaagcg gcaaaggaag agagaatgaa acagcgcgca
     5881 gaagcgaaag aagcaaggcg cttagctgca aaagaagctg cagaaaaaga agctgcagaa
     5941 tatgagaaga aaaaagcggt gagccagcaa agggaaaata ctgctgatga atggcaggaa
     6001 ccagaaaata cagagccgga gcagctaagt tttgttccaa tcgacagttt tcaagaaaat
     6061 atccatcctg cgaatctaga gaaaccagta ccagatacac caaaacagac caatacagca
     6121 gaaggttttt cagatgagtt gccagaggat gacggtacct cattagaatt tgagattgaa
     6181 gcagaacagg aaaatcaaga ctatgaatta ccgacagttg atttgcttga ttctatacca
     6241 actgtggatc aaagtgatga gtataaaaaa attgaaaaaa atatcggcgt attagaacaa
     6301 actttccaaa gtttcggtgt cgatgccaag gtcgtcaaag ccagcttagg accatccgtc
     6361 acaaaattcg aagtccagcc ggctgtaggg gtaaaagtaa gtaaaattgt gaacttaaca
     6421 gatgatatcg cgcttgcttt agcggcgaaa gatgtccgga tggaagcacc gattccaggt
     6481 aaatcactga ttggtattga agtaccaaat ggaaagatca gtatggtttc ctttagagaa
     6541 atcatcgaag cacaaccaaa ccacccagat aaattactag aagtaccttt aggacgtgat
     6601 gtttctggac gggtacaaac ggctgatctt tcgaagatgc ctcacttact cgtggctggt
     6661 tcgactggta gtgggaagtc agtagcaatt aatgggatta tcacaagtat attaatgcgt
     6721 gctaagcccc atgaagtaaa actgatgatg atcgatccga aaatggttga attgaatatg
     6781 tacaacggca tacctcatct gttgacgccg gtcgtgacga accctagaaa agcagcgcaa
     6841 gccttacaaa aagtagtaca agaaatggaa gaacgatatg aaaaattcgc ggcaaccgga
     6901 gtaagaaata tttctggtta caatgaattt gttcaacaga aaaacttgga aaacggaacg
     6961 aaacatccaa ccttaccatt catcgtagtg atcgtagatg aattagctga tttgatgatg
     7021 gtagccagca atgaagttga agatgcgatt attcgtttag cgcaaatggc tcgggcagcg
     7081 gggattcata tgatcctagc tactcaacgt ccaagtgtgg atgttatcac aggaatcatc
     7141 aaagcaaatg taccttccag aatggcattt gcggtatcaa gcggtacaga ttctcggacg
     7201 atcattgatt ctaatggggc agaaaagctc ttagggcgag gagatatgct attcttgcca
     7261 atgggtgaga ataaaccgat ccgtgtgcaa ggagcattca tttctgacca cgaagtagaa
     7321 cgggtcgttc aatttgttac agatcagcaa gaagctcatt atgaagagaa aatgatgccg
     7381 acagatgaag ttgaaacgac aggtgctcca gaacagccac aagatgagct gtttgaagaa
     7441 gcaaaagcac ttgtcgttga aatgcaaacg gcaagtattt cccttctcca aagaagattt
     7501 cggatcggtt acaaccgagc agcacgttta gtagatgaac tggaagctca tggagttgtt
     7561 ggcccgtcag aaggaagcaa gcctcggaaa gtattcattg agcaggaaga aacatcagaa
     7621 acgttgccgg acgataatga catgtaaaac ttgtgtgatg aagggttgaa aaacgaataa
     7681 ccaataaaaa gacaaggcat gatgatacca aaagaaaaaa tggttttttt ccatcttttc
     7741 taatacggta tgatgtcttg tctttttagt ttttgtgaga ttaaagttct attcgattta
     7801 tttgtgaagt attgcattaa ggaaatgaat catgtatagt atgtagcgaa actttgagaa
     7861 aaagagggaa aatactttgg aaaaacagtc catttatggc ttgacgaatg aagaattaat
     7921 caattggttt atagaaaacg gcgagaaaaa attccgagct gcccaagttt gggaatggtt
     7981 gtatcaaaaa cgtgtaagta atttcacaga aatgacgaat ctatcgaaac aattgatcga
     8041 aaaattatcg gagcatttta tcatcaatcc tctaaaacag atggtcgtcc aagaagcttc
     8101 ggatggtaca gtaaaatatt tgtttgaact ccctgataaa aacatgattg agactgtact
     8161 gatgagacaa gaatatggct tgtcagtttg tgtgacgaca caagtaggat gtaatatcgg
     8221 ctgtacgttt tgtgcaagcg ggctgctcaa aaagaaccgt gatctgactg ctggagaaat
     8281 tgttgcacag atcatgatgg tccaacatta ttttgatgag cgcaatcttg gcgaacgcgt
     8341 ttcacatgtc gttgtaatgg ggatcggtga gccatttgat aattacgaca atgtaatgga
     8401 ctttttgcat atcatcaatg atgccaaagg attggcaatt ggtgctcggc acattacagt
     8461 ttccactagt ggattagcac acaaaatcaa ggaattcgct aataacggct tgcaagtcaa
     8521 tctggctatt tccttgcacg cgccgaataa tgaggtgcga acatcgatca tgcggatcaa
     8581 ccgaagtttc ccaattgaga aattgatgga agctgtggat gagtatttag agaaaacaaa
     8641 ccgtcgaatc acgtttgaat atatcatgtt gaatcaagta aatgatagac cagagcatgc
     8701 gcaacaatta gcagatctat taaaagataa gaaaaaatta gcatatgtca acttgattcc
     8761 atataatcca gtcagtgaac atgatcaata ttctcgcagt ccaaaagctg atgtattgag
     8821 attttatgat gtattgaaga aaaacggcgt aaactgtgtg atacgtaaag aacatggtac
     8881 agatattgat gcagcttgcg gacaattgcg aagcaagcaa atgaaaaaaa cagttgccaa
     8941 ataatcgtat cctactcgtt ttttcaatta ccataaaagg agcagttttc ttaattaagg
     9001 aactgttcaa cttaaataag agaaaagatt ctatagcgaa agctataaag ctttttttga
     9061 caagaaaaac cctcatcaag caaagaaatg tcctcaattt gatgacagcc tgcttgatgg
     9121 gggttttagc aattatttaa ctgattttct tatatccttg ctggctttct tgtgtagcat
     9181 gataagtttc tacgtcaaga atggcctgtt taaatttttc tcgggtgatt tcataaggca
     9241 catgtttaat atcatttgtt gccatggctt tatctaagat tgcttctaaa tcggtcgtga
     9301 cattgagatt catgtcggtt aacgatactg gaagttggtt ctttttcata aacacataga
     9361 ttttatcaaa caaatcgaac tgctgatcca tcatcaataa ggtcagtaca ccataactga
     9421 cagatgctcc gtgaaggtat ccctccattt tttctagaac agtcgtaccg taatagaatg
     9481 agtgtgccat agcagaatta tagttattgt taactaaaac agaaaccaat ccggtagtaa
     9541 caatgatatc cgaaacgact tgttcaattg catatgaggg acgatcgtcg gcactatctt
     9601 tcaatgcttg cgtaccataa gtcaagagac gatcaatgca catttttccg atttggattc
     9661 ctaatctatc cgtgtagcct agatcatcgc cacgagcaga aaaagtagat tcgacttctt
     9721 tggataaagc atcgccaata cctgcccata agtatttatc aggtgcttcg gcaataattg
     9781 ctgtatcaat gaaggtgtgt acaggtggtt ttgaaggaaa gaagagtccg tccattacgc
     9841 cttcttcgtt atagatgaca cataccgcag tgacgggcga acagttcgaa ccaatagtgg
     9901 gaaaggtgaa gactggttta gctagttttt caccaatgac ttttacagta tcgcaagcac
     9961 ggccaccacc gactgcaaac aggacgtctg ctttttggac actttcatgc tggatcaact
    10021 gttcaatatt tccataagtt gaattacccc cgtaccagag actggcgaca acctctattt
    10081 cctgtgtcaa agctactttt attttgtttt ctgcctttga aagtgcagtc ttccctccaa
    10141 tcagcactac ttttgtccca tattctttga cgattccatt tgctttttca tacgcatctg
    10201 ttccaatggt ataatttggt aagaaaacac tttgactcat attcatttcc ttctttcttc
    10261 gttgaaacgg gttaaaattt taaagagtga tcgatctgac tgcggctgta gatggagtag
    10321 aggtgaacct aagacaggat aagtgatgaa taaggtggca tgaacacctt gaaatcatca
    10381 ataagtatcc taaaaacgaa caagtacaaa aacattgaaa acgacaataa tgaa
//
LOCUS       DAKBVG010000093        10228 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000093 DAKBVG010000000
VERSION     DAKBVG010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.093"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            161..310
                     /locus_tag="OUD09_001626"
     CDS             161..310
                     /locus_tag="OUD09_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HCT9692899.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            321..1835
                     /gene="dltA"
                     /locus_tag="OUD09_001627"
     CDS             321..1835
                     /gene="dltA"
                     /locus_tag="OUD09_001627"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HCT9692900.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1832..3037
                     /gene="dltB"
                     /locus_tag="OUD09_001628"
     CDS             1832..3037
                     /gene="dltB"
                     /locus_tag="OUD09_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HCT9692901.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            3082..3315
                     /gene="dltC"
                     /locus_tag="OUD09_001629"
     CDS             3082..3315
                     /gene="dltC"
                     /locus_tag="OUD09_001629"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HCT9692902.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            3318..4586
                     /gene="dltD"
                     /locus_tag="OUD09_001630"
     CDS             3318..4586
                     /gene="dltD"
                     /locus_tag="OUD09_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HCT9692903.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4727..5809
                     /locus_tag="OUD09_001631"
     CDS             4727..5809
                     /locus_tag="OUD09_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HCT9692904.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5809..6612
                     /locus_tag="OUD09_001632"
     CDS             5809..6612
                     /locus_tag="OUD09_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HCT9692905.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6682..7422
                     /locus_tag="OUD09_001633"
     CDS             6682..7422
                     /locus_tag="OUD09_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCT9692906.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7433..8437
                     /locus_tag="OUD09_001634"
     CDS             7433..8437
                     /locus_tag="OUD09_001634"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HCT9692907.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(8482..10179)
                     /locus_tag="OUD09_001635"
     CDS             complement(8482..10179)
                     /locus_tag="OUD09_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9692908.1"
                     /translation="MKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLE
                     KPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTL
                     TVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMY
                     KQKRLEYSLILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYY
                     WSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLAC
                     IGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISY
                     NLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLM
                     HTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLP
                     EELPINDDDLAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYD
                     TNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT         3282 a         1693 c         2079 g         3174 t
ORIGIN      
        1 gagaaattat aaagaaaaaa gcagtgattt gatgtgctat ttttataaaa gctatgtgac
       61 aaaactgtta gtaaaaaaac ggtttctgta agctagacac atgtttcttt ttgtcaaaat
      121 tagttacact ttgtgtgagg ttatatgagg agggaatcca atgaacaaat ggcgttctaa
      181 tccaaaattg aattattggg gaaaattcat cattcgaaca attttttata gtgcagtact
      241 gctgttcctg atttatctgt atcactataa aaatattcaa ggaggaactt ttatctataa
      301 cgaattttag gaggaagagt atggaaatca aaacgattat tgaagcaatc gatgattggg
      361 gaataaatga acctgatcga gttgcttatc aggcggatga gacgcatact tttggtgaat
      421 tgaaacaagc atcagacgca cttgcttatt atttagaaaa aaaggtagaa ggcgatggac
      481 caattgtcgt ttttggcaat ttggagtttg aaatgattgt ctcatttcta ggggttgtca
      541 aatctggcca tgcctatttg ccgattgaag agcatacacc caaggagcgt atcctatcga
      601 tctttcgggt ggctaaacca tctatggtca tcagtatcgg ggactggata gaagatattc
      661 cttcggttcc tgtcataaca aaaaaagaat ttcaagaaat caagaagata ccagtaggat
      721 tttatcccag aaatagcgta aaaggtacag aaaattttta tattattttt acttctggta
      781 ctactggaga accaaaaggc gtacaaatca gtcatgataa tctagtcagc tttgttcaat
      841 ggacgatgga agactttggg atcaagcctg gtatgcattt cttagcccaa gcgccatttt
      901 cttttgattt atcggtattt tctatttatc ctgcattagt ttctggcgga atgctgaagc
      961 cgttaaataa agcagttgtt caagactttc gtcaattatt cgcgacattg ccagccttga
     1021 agttgaatgt gtgggtttct actccatcat ttatggatat ttgcttgatg gaacctacat
     1081 tcaatggaga aaatgtgccg gaattggaca tgtttttatt ttgcggagaa gaattgacga
     1141 aaaaaactgc agaaagtcta cttgatcgat tcccaaatgc acggatatat aatacttacg
     1201 gtccaacaga agcaactgta gctatttcaa gtattcagat tgaccagcat gtactagata
     1261 gttatgaccg actaccgatt ggctacgtaa aaaaagacac gaaagtttct attgtccaag
     1321 aaggtaaacc tgtttcaaaa ggagaaacgg gtgaaatcat tattgcagga ccaagcgtat
     1381 caaaaggtta cctgaacaat cccgaaaaga cagctgctgc gttcttttgt caaaatgact
     1441 tagcaagtta ccatacaggg gatgcaggta gactagatga atatgggctt ttattttatg
     1501 aaggacgtat ggatcagcag gtcaaattac atggataccg gattgaactc ggggatatcg
     1561 aacattatct gctccaagac aaccgaatca aacaagctgt cgtcgtacca aaataccaag
     1621 gaacaaaagt acagcagctg gttgcttttg tcgtattaga aaacaaaacg aaacagccgg
     1681 attttcagtt gactaaatct atcaaagaac aattactaga agttgtaatg gattatatga
     1741 ttcctcagaa gttccattat gtcgatctgc tgcctcagac agttaatgga aaaatcgatc
     1801 gaaaaaaatt aacagctgag gtgaatcctg aatgatgtta cctcatatga tcccttatgc
     1861 agagccgttt tatttttttc ttttagccat tgcgcttgta ccgatcgttc tatctttatt
     1921 gatatgggaa aaaagattac cagtttatca atcagcagtg acacttttct ttttatttgt
     1981 cagctttgga ggtaattatt ggcaacaggg agcggcgctg attgggtatg ttttgtggca
     2041 gacgatactt gtatggggct attttgttta tcggcaaagg aaaaatcaga caggatggtt
     2101 ttttgccgcc gttattttag cgataattcc actgttttta acgaaagtga ctcctttttt
     2161 tgaacatgga aaagcatcgt tacttggttt tttaggaatc tcttatttaa cctttaaatc
     2221 tgtacaaatg atcatggaaa taagagatgg catgatcaaa acatataagc catttcgtta
     2281 tatccagttt ctgctgttct ttccaacaat ctcttctgga ccaatcgatc gctataggag
     2341 atttgaaaaa gatcagctcc atccgcctgt tcgtgataaa tatgttgatc ttttgggaaa
     2401 aggcgtccaa aatatttttg tcggtttttt atataaattc attatcggtt attattttgg
     2461 tcaagtactg cttcctgtag tgggacgcat tgccttacag catggtggta tttcatgggc
     2521 attagtaggt tatatgtacg tgtacagtat gtatttgttt tttgattttg ctggctatag
     2581 cttatttgca gtcggtacaa gctacatgat gggatatcag acaccaatca atttcaataa
     2641 acctttttta tcatggaata tcaaagaatt ctggaatcgt tggcatatga cgctgtcctt
     2701 ttggttccgt gattatgtat atatgcgatt gatgttcttc atgatgaaga aaaaattttt
     2761 taaaagcaag atcgtaatgt caaatatcgg ttattttgct ttgtttcttc taatgggtat
     2821 atggcatgga ttgacgtggt actacatcgt atatggactg tatcatgctt gtctgatctg
     2881 tctgacagat gcatggatcc gatttaagaa gaagcataaa gagcagttgc cttctaacaa
     2941 atttacgcat ggcttagccg tatttttgac atttcaagca gtatgtttta gcttcctgat
     3001 tttttcagga tttttagata aacttttttt tagctaacga atagtgacat attaatttat
     3061 ataacaaagg agaaaaggaa aatggaagaa caaattttat caatcttaga agaaataaca
     3121 ggaacagatg aagtaagaaa aaatcgtgat attgatctat ttgatgaagg gttgcttgat
     3181 tctttagcat ctgttcaatt attagtcgag ctagacggag agctggatat ccaagtccct
     3241 gtatctgaat tcgagcgaga ggattggagc acaccgaata aaatcattca acaagcaact
     3301 gcattgaagg gttgagtatg aaaaagaaac tttttgctat tttcggcccg gttgttttag
     3361 ctgttggctt actgtttgtg ttttttacct cctcctatca agtagatatc tctaatccag
     3421 aaacgattag acgtgcctct tcttccatgt cagtgaacgt gttgaaagga gatgcgataa
     3481 aaaattatgc attatctgaa aaacaatata ttcctttttt tggctcaagt gaattaagca
     3541 gaatcagtcc atttcatccg tctgttctgg cagaaaaata ccagcgaaac tatcgtccat
     3601 ttttgctggg agcacccggg acacaatcgt taagccaata tatgatgatg cgttctgcag
     3661 gagatgctat gaaaaacaag aaagtcgtct ttattatttc tcctcagtgg ttcgttaaaa
     3721 atggtgtaaa aactgattac tttaatacgt attattctga acttcaaaca tacgactggt
     3781 tgttttcaat gaagaaagta acgcctgctg atcgctattt agctagaaga ttattaactt
     3841 tctcaaaagt aaaagagaat gatacgttga cggcgattct gcaaacgatc aaaaaaggga
     3901 agctaccttt acctgaatct ttgaatcagc tgcgcagcca atggaacatg ctgaagagag
     3961 aggatgaagt attcagcaat attgggttga aagatcgaca gcaaaaaatc gatcatgagt
     4021 caaaacgatt accaaaacaa taccaagaaa cagaattatc cattttagca aatcaaattg
     4081 gagaacgaga aacgacgaat aatccattcg ggttgaaaaa cgatttttat acgcatcgta
     4141 tccgagccca cgagccagag ctcaaacagt cacaaaaaaa ttgggattat cgtttttcgc
     4201 cagaattttc tgattttcag ctggtcttgg atcaattagc aaaaaaccat aacgaagtat
     4261 tgttcatcat cccaccggtt aacgagaaat ggagcgatta taccggacta tctcaagaaa
     4321 tgttacaagg atttgccaaa aagatcaaat ttcaattaaa tagccaagga ttcaatcgaa
     4381 tagctgattt tgtcaatcaa gcgggtacca actattttat ggaagacacg atccatctag
     4441 gctggaaagg ctggttggct gcagatcaac agatcagacc gtttttagaa gaaaatcata
     4501 ttactgctag taagtatcat cttgacgatg ctttttttag caaatcttgg caacatcaaa
     4561 taccagataa actacaatta aaataaagaa aacaggcgag tgttgctcgc ttgttttctt
     4621 tatttttgaa tattctgaat aaaagataaa aataccaagt ttgcctgaac aaaaatcata
     4681 agcaatggta taatcacaat atgaatatag aaatggggga aaacatttgg atacaaaaga
     4741 agaacagcta cttattgatt taacttctgc aaaaggagta cctggaaatg aagaagaggt
     4801 aagagaagta ttcagagaat atgccaaacc attcgctgat gatatttttt atgacggttt
     4861 aggcagtgtg attgccaaac atggtgctgg cggaggcccc aaggtcttta tttctggaca
     4921 tatggacgaa gttggtttta tggtaacaaa aataaccgaa aaaggattct tggaattcca
     4981 aactcttgga ggttggtggg ggcaagtcat gcttgctcag caagttgaaa tcaaaacacg
     5041 tgaagggaaa atcgtccacg gtgtgattgg ttccaaacca ccacatgttt taacacctca
     5101 agtaagaaat aaaccttatg aaataaagga tatgttcatt gatattggcg catcaagcca
     5161 agaagaagcc aaagagtggg gcatacgtcc tggagatatg gtcactccat atattgaata
     5221 caaacgaatg aatggcagta aatatttact ggcaaaagca tgggataatc gtatcgggac
     5281 agctgtttct ctgcgagtat tagagaatct atctaaagaa gcacacccaa atgttttatt
     5341 cgcaggaagc gatgtgcaag aagaagtagg cttgcgaggt gctcgaacaa gtacacacct
     5401 tgtaaatccc gatattgctt ttgctttaga tactggtaca gcaggagaca ctccaggaat
     5461 gacaccaaaa gaagcagatt cgattttagg aaaaggccca cagattttga tttttgatgc
     5521 atctatgatc ccgcataaaa aattattgaa ttttgttatt gatgtagcag aggaaatgga
     5581 tattccattc caatatacag tgatttctgg cggaggaact gatgcaggac aaatgcattt
     5641 gacaagagat ggtgttccgt ctgtagccat taccgttcct gttcgttatc tgcattccca
     5701 tacatcagtg attcatgaag atgattattt gaatacagtg aaactagtca cagaattagt
     5761 gaaacggctt gatagtgaaa cagttgaaaa aattcggagt tatgaataat gaataataga
     5821 atacctattt ctgaatatcg gaaaaaagca cgagtttctt tggaaaatca atggggaatc
     5881 aatacttggc ttgttttctt atctataata tttacgagcg tcatccagaa cttttttgac
     5941 ggtttcagtc cttttccgga agaaagcatg cagcagcact tattgagttt tctagtcgat
     6001 aatttacttt tattcgcatt caactatgca ttgttttaca ttgcgctagt agttgtacgg
     6061 ggtggcagac caaaaagtaa ccttttattt tcagttttcc aaagaaagta ttttggttcg
     6121 atacttggca tcaatttgat caattcagtc atcaactggc tactcaacag tttaatcttt
     6181 ctgcccatgt ttttaatcag cggagtgagt acttatatgc agctgctttt cagttcaaat
     6241 gcggtaccta cgggtgtcat gccagaagat tttcttgata tcagctttgt tttatcgatg
     6301 atagggatga tttttgtttc agcactcatc atgagtattg ttggcggttt attccaattt
     6361 gctgcatgga caaagttcga ctatcctgat ttgtcggtta ttcaatgttt gaaatatggc
     6421 tggtttttat tgaaagatag aattggtacg tatattttgt tgcagctgtc ttttatcggc
     6481 tggtatttgc taggagcaat cgctttgttc atcggtcttt tgtgggtcat tgtttatgtg
     6541 aatgtgtcag ttgcagaatt ttatgaacaa gcacggattg aaaaaggagg tccagacgcc
     6601 tattttactt agcatgtaag cagtttattc atcaaaaaaa taacttttgt ggtaggataa
     6661 atctttgagg gggaatgtac gatgaaatta actgttttag gatgcttagg agcgtatcct
     6721 tataaaaatc aagggacaac aggttatttg cttcaatctg acgggttcaa tttgttgatc
     6781 gatgcaggta gtgctacgct gatcaaatta caagaacacc ttgatccact tgatctagat
     6841 gctgtgatcc ttagccatta tcatcatgat catattgctg accttggtgt attacagtat
     6901 tactggcaac ttcatcctaa gagaaatccg gagacattgc tttcaattta tggacacaca
     6961 aaagattcca ttcactttga agaattgacg ttggaaaatg tctcaaaagg acatccttat
     7021 tttgaagctg aagaattaaa aattggtcct tttctgatta cttttatgga aacaatccat
     7081 ccagtagtat gttacgccat gcgtatcgta gaagaaacaa cggggaaagt attcgtattt
     7141 acaggtgatt caggttattt ggaaagtttc gctgattttg cgaaagatgc tgatttgttt
     7201 ctagcagata cctatctttt tgaaggaaat gaacatcatc atgcccattt cacttcaaaa
     7261 gaagctggag aacttgcgaa atcagctaac gtaaagaaac ttgtacttac acacctacct
     7321 cagtttggtg atttagaaca attgaaaaat gaagcgcaaa aggcagcagg caaagggatt
     7381 gatatagaac ttgctgcagt tgacaaagtg tttaacattt aggaggaaga acatgatttt
     7441 tgtaccaaat gaaaaccacg acccaagaat caatttagca attgaacaat atttgctgca
     7501 agaaatgcct ttagatgaac ctattttgct cttttatatc aatgaacctt caatcatcat
     7561 cggcagaaac caaaatacgc ttgaagaaat caatcgagat tatgtggaag aaaatggcat
     7621 tcatgttgtc cgtcgcttaa gtggaggcgg agctgtctac catgattttg gaaacctgaa
     7681 ctttagtttt atcatgcctg atgatgggga ttcattccgt gactttgcga aagtaacaca
     7741 gccaatcatc caagcacttc atgaattggg tgttgctggg gcggaattga aaggccgaaa
     7801 tgatctagtc attgatggca tgaaattttc tggcaacgcg atgtatgcta ccaacggtcg
     7861 gatgtttgct catggaacaa ttatgttcga ctctgatatc aatgaagtag tcaatgcatt
     7921 aaaagtaaaa aaagacaaaa tcgaatcaaa aggaatcaaa tcgatccgtt ctcgtgtaac
     7981 gaatatcaaa ccatttcttc cagaagaaaa gcaaaaaatg acaacagaag aatttcgtca
     8041 agaaatcttg ttgaaaattt tcggcgtaga gtcagtagaa gaagtaaaaa cgtacgaatt
     8101 gactgatgaa gattggaaaa aaatcaatcg aatctctgag gaatattatc gaaattggga
     8161 ttggaattac gggaagtctc cagatttcaa tttctctcgc caaaaacgtt tttctatcgg
     8221 ttcgattgaa gtccatttga atgtctcaga aggaatgatc aaagaagcaa aaatctttgg
     8281 cgatttcttc ggactaggag atatctctga cgtggaaaaa aaattggttg gacagaaata
     8341 tgataaagca tctctatcaa aaatagtcag ccaaattgat gtgaagaaat attttggtgc
     8401 gatagaacca gaagaattgc ttaccttact ttattgaaaa taaaaaatag cggaatccac
     8461 atatatggat tccgctattt tttatacagg attcgggtaa tttcgagaga gttgaacagt
     8521 gatccgatca atgaaagtat ctttttcctg cgttagtgtt cctgtgtaac gatccgctac
     8581 gtctttcatc atcattaagt caaaacgttc ctgatcatct aattcttctg tgatgcctcg
     8641 actaaatagg tttgtatcgt aatgtgcact atgttcgcaa caaatgacga aatgttcctc
     8701 ttgttcgtgt atcgacaaat aaatttttcg atacagtgga ttttcgatgg catgagtttc
     8761 tctaaaggaa tgctctaaag cgtggatcaa aagttgagcc agatcgtcat cgttgatagg
     8821 aagttcttcg ggaagatctg cttggaaggt tatctgaata ttccgtttgg cagctaactt
     8881 ctggtaacgc gctaagataa gatttgtcac ctggtggttg caaaaagaac acagcatttc
     8941 gaaatttttt gcttcttcta atatcttttc cacatagttt ttagcttttg tttggtccgt
     9001 atgcattaaa tcttgcagat tctccatctc ttggacaaat tccaggcgaa gtgtatttac
     9061 ttcttggata tgctgtcgaa gctgctcata atgttgctcc aacaaatcat ttttgatttt
     9121 taagaaatct acttgtctcc gatctttgtc ggtatttaag atgtgatcga ttagattgta
     9181 gctgataata acgattagaa tgataatata taaaatcgta gaccaattga tcgttgtttg
     9241 atagatacct aatgccgttt ggatgtaaat caagcaatgg ataatggcta ccaagacgat
     9301 ccaaggtgta caagctacat aaaaacggtt attcttatat gcttcgccaa tgcatgcaag
     9361 tgaagtgact aaagtgctta taacactagc aatattagca atcggcatta attcaggaag
     9421 ttctgcagga gtaaaagttg cataaagcag aacgccgata aaaatcaaaa gttggaacgt
     9481 acaaaaaatt ccgtaccatt ttttatagta aaacattcgg ctccaatagt agcagatgat
     9541 gaaagctgac gttaagataa taaataaatg agataaaaaa gaatgaacga atggttcaaa
     9601 aagaatacca cttaaaatat cttctgatac agcttcaaat cctaatgcca aagcaaagca
     9661 acttaagaga ataagtgagt attcaagacg tttttgttta tacatcaagt agatcgtata
     9721 gaaaaaggtc cctatagcga taaaaacagc gattatcatc gtcaatactt gcggcactga
     9781 ttgtgaataa ataaaactaa tcaatggtcc agttgttcct agataaactt tcggtggcag
     9841 tccagcatag ttctgataag gagatgagac tgtaagggtc aatttttttc cgatataatt
     9901 tgctggtaaa tctatcacag ccaatgagag tccgggattg ctttcttcct gataatcttt
     9961 ccgttggtat aaaatctttt cgcctagata tacagtaatc cattgatggt ttcctttgat
    10021 tagtaaattt gcttcgtcca acggtttttc aagtgtcgtt tccataatga atgtttcgtc
    10081 tttatccacg gtaggaatcg tacgagtgta cttgctgtca aacttaaaat caggatcatc
    10141 ttttgtatag aaaacccacg aatcttttat ttctttcatt tgattgctgt ccgcaggaga
    10201 aaaaataaaa taaaagaaat aagcaatt
//
LOCUS       DAKBVG010000094        10160 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000094 DAKBVG010000000
VERSION     DAKBVG010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10160
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.094"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="OUD09_001636"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="OUD09_001636"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCT9692909.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="OUD09_001637"
     CDS             complement(1172..1867)
                     /locus_tag="OUD09_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HCT9692910.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="OUD09_001638"
     CDS             complement(1880..2596)
                     /locus_tag="OUD09_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HCT9692911.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3531
                     /locus_tag="OUD09_001639"
     CDS             2926..3531
                     /locus_tag="OUD09_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HCT9692912.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            3856..4179
                     /locus_tag="OUD09_001640"
     CDS             3856..4179
                     /locus_tag="OUD09_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HCT9692913.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4361..5731
                     /locus_tag="OUD09_001641"
     CDS             4361..5731
                     /locus_tag="OUD09_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HCT9692914.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5861..7162)
                     /locus_tag="OUD09_001642"
     CDS             complement(5861..7162)
                     /locus_tag="OUD09_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCT9692915.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(7392..9350)
                     /locus_tag="OUD09_001643"
     CDS             complement(7392..9350)
                     /locus_tag="OUD09_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HCT9692916.1"
                     /translation="MAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9527..9793)
                     /locus_tag="OUD09_001644"
     CDS             complement(9527..9793)
                     /locus_tag="OUD09_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCT9692917.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
BASE COUNT         2980 a         2100 c         1851 g         3229 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcgaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 agcacaggtg aaggtggcga accagaagtg atcggttcaa cagaagaata atactgaaac
     3541 aatcaggtgg tgttagaagc ggcgggtcag cagcttttaa cacgcctttt ttatttctaa
     3601 tcgtttattt tcatatgggg cttccacttt tgtaaatggc tttacaaacg aaaaatccta
     3661 atttaccaag cttttcctcg catactttta tcgtaccttt ataaaaattt atactttgaa
     3721 aaaaagtatt caattattgt tgcatttttc ctgattcttt attagaatga accctcgttg
     3781 aagtaagtat ttctagttta tcctaggatt aattataaaa ataaaacatt cgttgattcg
     3841 aaggaggtta aacagatgtt tgaaagtttt gatagcagcc gcaaccgtta cgcttcagtt
     3901 ggtgtcgtat caagtttgcc agatgaattg attgacagta tatggtttat tattgaccta
     3961 gatcttaagg gagtcattcc attagaaaat atattaacct ttgatttgat cgaaaatcgt
     4021 ggaaaagtga cgatgcattt ttctcaagca ggaagcaatg tggaaatgga tattgatttg
     4081 ccgtttgcct attcgaacca atttcctgct caagtatacg cctatgatga cggtacgaga
     4141 gaaacgatcc ttttaccaag tgagattaga caatattaag gtgatagata aacgtggatg
     4201 tcccttattt tacagaccag gccaagttgt tgattctttg actcaattct tggcttcttt
     4261 ttatggagtt tcataaatta tcattgattt ctaattggta ttctcaggat aaacgctata
     4321 tactactaac taaattttaa agatataagg agtttaaaag atgcgcgatt taacaaaagg
     4381 aagccccgct aaactgattt tgatgttcac tgttccttta ctgatcggaa atgtttttca
     4441 gcagttctac aatatgatcg atatgatcat cgtgggtcag acgctaggta aaaatgcgct
     4501 ggcagcggtt ggtgcaactg gaagcctgac atttttgatc attggctttg cccaaggttt
     4561 aacagctggt ttagccatta ttaccgcaca acgttacggt gcaaaagatt atcggggtct
     4621 gaagaaaagt tttgctgcta gtgttgtcat tagtcttgtc gtcactgtga ttttaactgt
     4681 cctaagtctc ctgtttattc gtccgatgct tcaattgatg caaactcctc ctgaaatact
     4741 tgatcaagca caaacattta tttcaattat tttattaggg atctttgcat cagtcagttt
     4801 caatttgctt tctaatgtca ttcgcgcact tggtgacagc cgcacaccac tattctttct
     4861 gatcattgcg gtcatcatca acgtcgtact tgatttgatc tttattatct attttggcat
     4921 gggtgtggaa ggtgcagcta ttgctacagt aatcgcacaa gtttcttcca gcgtactgtg
     4981 tctagtgtat atcaagaaaa aaatgccttt gttacagtta cgcaaaaaag atttcaaatt
     5041 tgaaaaagaa gagtttgctg tacacctaaa tgctgctttg ccaatggcgt ttcaatcttc
     5101 gatcatcgct gttggtgcta tcgtgttaca agcagcacta aatagtctag ggacagatgt
     5161 cgtagctgct caagccgctg ctggtcgtat cgaccaattt gcaaatcagc cgatgatgtc
     5221 atttggtatt gcaatggcaa ctttcactgc acaaaactat ggtgcgaaag aatatggccg
     5281 aatcttgcaa ggggtacgtc aaactttact gatgagtgtc ggatttagtc tcttagcagg
     5341 tgctatcgtc attttcttcg gtcatttctt tgttcaattg tttgttagcc catcagaaac
     5401 acgtgtattt gaattagcac aaacctactt caatatcaat gggtctcttt actggatctt
     5461 agcagtcctg ttcattttac gctacacact tcaagggctt ggacaaagca agatccctac
     5521 gatcgccggt atgatggaat tgcttatgcg ttcttttgca gctatcgttc ttacaggaat
     5581 gtggggatac ccaggagcag cagcagcttc tcctttagct tggatcggtt ctgttgccgt
     5641 gttgctttac tcttatatgc gttcgatgaa acaattgaaa cacatgggtg aagaggaaca
     5701 tttcacatta gaacatgcag aagcaagata gccttgatta aaagaaatcg ttgcggctaa
     5761 aaatgaaaac ttttattgaa aatatcttct tcgtttaccg aaaacaggtt ttttagataa
     5821 caaaaaggct gtgacaaaaa atgtcacagc cttttttctt ttattctatt tttctttccg
     5881 tggactctac tttgaaataa tcgatatgtg catgctggta ttctaatacc gtgaaacgga
     5941 atgatgcaat ctcaatgatt gttcctgctt ttatttcttt atatcggcta gaagcaaagc
     6001 ccgcaagtgt agtaaaattc gtttcttcaa actctggaat ctctacatga aaataacgct
     6061 ggaaatcatc taatgttagt ttgccggaga tgatataaga gccatctgac tgttccgcta
     6121 tcaatgcttc gtccaaatct gtctcatctc ggatttcccc gacgatttct tctaaaatat
     6181 cttcaatcgt cactaagccg cttgtccctc cgtattcatc ttttaagata gcaatatgct
     6241 ttcccttttt tcgcatgatc gtcaataatt tttttatagg gatcgtttca gttgttacga
     6301 tcggctcttc aatcaactgt tctactgtca gctggtcatc tttataagat tgcttgatca
     6361 aatctggcaa ggtgacataa cctaaaacat tgtcttttga ttctaagata accggatagc
     6421 gagtatgtcc ttgttcaatc gaagctttta tcgcttcttt gacggtttct gaagcttcga
     6481 atacttgcat ggacgtacgg tctacttgta tatcttttgc caaggtatca tcaaattcaa
     6541 acacgttttc caaataatga tattcttctt tggtcaactc tcctttttca taagaattca
     6601 atgcgactaa tgtcagctct tcttgagaca atgtctcttc tccttcagat acgagacgca
     6661 cacctaatag cttccctagc cctgcagctg aatgattcaa tacccaaata aaaggaaaca
     6721 ttactttata aaaataatga agtggtttaa ctacgaacaa gacgaccttc tccgtattga
     6781 cgatactata gctcttagga agcagttcgc caataaccac atcgatatac gtaactaaaa
     6841 taaatcccag cgccacagag attgtactgc taatagccga actaaaaggt agtttaccga
     6901 tcactggttc tagtaaagcc gcgacagatc cttcaccgac ccacccgatg atcaaaccgg
     6961 ttagcgtaat tcctaattga ctagctgata aatagttatc taaatggtgt gtaacatgaa
     7021 gagctagctt ggcgttcttt acattttcct tttctagttg ttccaatctt gatcgtctga
     7081 ttttcactaa agcaaattca cttgctacaa aaaatgaagt aattgcaatc atcaaaaata
     7141 aaataatgaa attgataacc atattctccc gcctttctct ttttctattg tacccttttt
     7201 ctacaaaaag attgaaaata atgcgtattc atatttagga ataaaaaagg agatgcggca
     7261 tttttcagcc acatctcctt atcacttcaa aatcattgcg ttacttgttg ctaaacagct
     7321 ttttcatgac ctctgataaa cggtgttcaa gtagctgaca ttcgacatta gttcaggtca
     7381 aagcgctact ttcacaaccg ctctttcaac catgttttcc gagtatttcc aatgataagc
     7441 ggaacagttt catgttttac ttcgctgtac tctagcccaa accatgtttc tggcgaggta
     7501 gcgatttttt tgattgccag ttttgcctgc atgatttgac gttgccattt tcctagagtt
     7561 gtcacattgg aaactacttc atcaatcagg acaaattgga aatcgccaac ttctcgccct
     7621 ggtgtcagag agtatctttg tggctgtttt ggcaaacggc cttgtttcat caaatcatgg
     7681 atgacttggc gaacatatac gttgatttcc tgttggaccc tgaaacctaa gaacagattc
     7741 acttggacaa taaaatcagt gtccatcata tctacttgat attctttcgt atatggctca
     7801 tccgtcactt ccacatgaac aaaccaatag actcgtgctc ttttaggccg tttatctaaa
     7861 atcgaataga tgaactgtcg gccaacttta tcttcagcca tatcaggaac caagaagacc
     7921 acatttgtct gatagattgg taaagaagta tcatcacgta attctctaag ctgcgggata
     7981 taatctctca aagacacatc ctcggttgac gattcgcgaa taatgttccc ttgttcccaa
     8041 ataaacatca cgactaagat gatcaaacca atcagaacgg ctacaaaacc gcctcggaag
     8101 aatttgacaa cacttgagat aaagaaaacg acttctatcg caccgaagaa aaaggtaact
     8161 aaatatgcaa gccaacgaga atgcccattt tggataagat aaaagtaaag cagtgctgtt
     8221 gtcatcaaca tcgtcacagt gatcgataga ccgtaagcag cttccatatg atgagacgtt
     8281 cggaatgtga caaccactag agaacaggcg atccataaaa tcagattgac tgctgggata
     8341 tacatttgcc cgatactgtt cccaggatac atgatcttta accgaggcag cagtttcaac
     8401 ttgattgctt ctgaaactag cgtaaacgaa cctgtaatca atgcttgcga agcaatgact
     8461 gcagcgactg ttgcaaaagc aacgccgatc acgatccaac tttgaggcat catttggaag
     8521 aatggattca ggttcttgat ttgctgtgct tcagggtttt gataaacatt caacaaccac
     8581 gcagcctgcc caaaataatt gataaccaaa caaattttga cgtaaggcca gctcagacga
     8641 atatttcgtt tgccgacatg tccaagatcg gaataaagcg cctctgcacc tgtcgtagcc
     8701 aggaaaatat ttccaagaat gaacaagcct aatttgttat ctgcactaaa aagcagctgg
     8761 atagcataat aaggattcag tgcacggata actgtccagt cttgactgaa gttcattaat
     8821 cccattacgc ctaaaaaagt aaaccaagcg aacatgattg gtccgaatgc ttttccgaca
     8881 atatctgtac caaagcgttg gaccatgaat aaaacaaaaa taataactaa agtgataatg
     8941 acgatcacgt tctggttatt accgaaccgg tcaaaaaact gcggaattcc tcgcaagcct
     9001 tcaatcgcag tcgttaccgt cacagcaggg gtcaataccc catctgctag taaggcagct
     9061 cctccgatca ttgcaggaat gatcaaatat ttcccacctt ttcggacaag tgtgtagagt
     9121 gagaaaattc caccttcacc atggttatct gcattcaatg caatcacgac atatttgatt
     9181 gttgtcagta gagtcaatgt ccaaaaaatc aatgagactg atccgataac aaaatcagga
     9241 ttgattcctg acaatccgcc attatcttca acaatcgatt tcataacgta aagcggactt
     9301 gtcccgatgt ctccgtagac gactcccatc gctacaagca ggccagccat tgatatcttt
     9361 ttggtattgt gcgttgttgc cacgtttttc cctcatttct tgttatatgg atatccttta
     9421 ggtaagcttg aatgatagat gatattctta tccatgacac ccgctttcct aaattgcttc
     9481 taattatcca agtaacacac tgaagtgtca acttatttga ataaaattat tctttctttg
     9541 gacgttctct gatacgtctt gccacaaatg aataaagagc aaggcaaaaa attgcaccca
     9601 ataatgctgg tatcaaataa aaaccgccga gttccggtcc cagtggtcct agtaaaaatt
     9661 cgccgagcca actacctaaa aaaccgataa tgatatttcc agatacgcct cccgggactt
     9721 cctttcctaa aattgcgcca gctatcaaac ctaatatacc gccgataaat aacgaccata
     9781 aaaaattcag catctccttt ccctccttta tccatatttc tcacttttat tttacgcttt
     9841 attcagaagg actccaaaaa tatgcgccaa gaaactccag aaaaaaagaa gttgtaacaa
     9901 aagtgttcag cttatttccg tagaagctgc ttctggaaca ccgtgaatca gaggttgtga
     9961 aatcagcggt ttgacctgag tattagagaa acaaacggaa aaatagttcc tcatattttg
    10021 ccgttttttg agctaatatc gagggacagc tgattgaaca tcgtttattg gagaggttgt
    10081 aaaaaagctg ttctgcatca agtaataaga acagccaagt aaaaacagct cctaatgttt
    10141 ttaacttggc tggacttatt
//
LOCUS       DAKBVG010000095        10143 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000095 DAKBVG010000000
VERSION     DAKBVG010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.095"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            13..243
                     /locus_tag="OUD09_001645"
     CDS             13..243
                     /locus_tag="OUD09_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HCT9692918.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            340..1641
                     /gene="murA"
                     /locus_tag="OUD09_001646"
     CDS             340..1641
                     /gene="murA"
                     /locus_tag="OUD09_001646"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCT9692919.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1654..1830
                     /locus_tag="OUD09_001647"
     CDS             1654..1830
                     /locus_tag="OUD09_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCT9692920.1"
                     /translation="MSNQRYILLTLIKILMVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            1965..2261
                     /gene="yidD"
                     /locus_tag="OUD09_001648"
     CDS             1965..2261
                     /gene="yidD"
                     /locus_tag="OUD09_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCT9692921.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            2446..2913
                     /locus_tag="OUD09_001649"
     CDS             2446..2913
                     /locus_tag="OUD09_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCT9692922.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            2998..3882
                     /locus_tag="OUD09_001650"
     CDS             2998..3882
                     /locus_tag="OUD09_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCT9692923.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            3996..4232
                     /locus_tag="OUD09_001651"
     CDS             3996..4232
                     /locus_tag="OUD09_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HCT9692924.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4320..4400
                     /locus_tag="OUD09_001652"
     tRNA            4320..4400
                     /locus_tag="OUD09_001652"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4354..4356,aa:Tyr,seq:gta)
     gene            4407..4478
                     /locus_tag="OUD09_001653"
     tRNA            4407..4478
                     /locus_tag="OUD09_001653"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4439..4441,aa:Gln,seq:ttg)
     gene            4603..4773
                     /locus_tag="OUD09_001654"
     CDS             4603..4773
                     /locus_tag="OUD09_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9692925.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVRVKSKRP
                     TNHTKKWMAKNR"
     gene            4993..5700
                     /locus_tag="OUD09_001655"
     CDS             4993..5700
                     /locus_tag="OUD09_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCT9692926.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5759..6403)
                     /locus_tag="OUD09_001656"
     CDS             complement(5759..6403)
                     /locus_tag="OUD09_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HCT9692927.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDEAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGIPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6529..6816
                     /locus_tag="OUD09_001657"
     CDS             6529..6816
                     /locus_tag="OUD09_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9692928.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            6924..7244
                     /locus_tag="OUD09_001658"
     CDS             6924..7244
                     /locus_tag="OUD09_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCT9692929.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7325..8638
                     /locus_tag="OUD09_001659"
     CDS             7325..8638
                     /locus_tag="OUD09_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCT9692930.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLSNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKIL"
     gene            8635..9354
                     /locus_tag="OUD09_001660"
     CDS             8635..9354
                     /locus_tag="OUD09_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HCT9692931.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVNSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQIAQPKIIRTISLAR"
     gene            9482..10033
                     /gene="raiA"
                     /locus_tag="OUD09_001661"
     CDS             9482..10033
                     /gene="raiA"
                     /locus_tag="OUD09_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCT9692932.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTANAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
BASE COUNT         3385 a         1682 c         2179 g         2897 t
ORIGIN      
        1 aaggacgtca ttatgcaagt atacggaatt gatgcggtgc ttcgaatcgt gagtcatttg
       61 gcgtttatct acttagcctt ctggtcttta cgttcgttac gcattgaaac ttttttcaga
      121 ccaatgcata atacgcaaat tagaatggca atcgtacttt tttcaatttt tttagggtac
      181 actgcaagta attttttctt agaagttatc gctttatgcc gaaatctttt cgttagcctg
      241 taaattttaa aaaaaggtac tattttaaaa gcaaggatgt tataatagaa aagattttga
      301 gtaagtgtat gctcattgga attttttgga gggaacaaca tggaagagat catcgtaaga
      361 ggtggcaatc aattaaatgg aacagtaaga attgaaggag ctaaaaatgc tgttttgcct
      421 attttagctg ctagtttgtt agcagaagaa ggtattacga cattagacaa cgttccgatt
      481 ctttctgatg tcttcactat gaatcaagtg attcgtcact taaacgtaga tgtagatttt
      541 gatgaacaaa agaatcaagt aacaatcgac gcttcaagac aattagaaat cgaagcgcct
      601 tatgagtatg ttagtcaaat gcgtgcttca atcgttgtga tgggtccgct acttgccaga
      661 aatggacatg caaaagtagc tatgcctggt ggatgtgcga ttggtaaacg accaatcgat
      721 ttgcatttaa aaggatttca agctttagga gcaaaaatta ttcaaaaaaa cggctatatc
      781 gaagcaattg cggatgaatt gatcggtaac acgatttatt tagatttccc tagtgtaggt
      841 gctactcaaa atatcatgat ggctgccgta aaagcaaaag gaacaaccat cattgaaaac
      901 gtagctcgcg aaccagaaat cgttgattta gcaaacattt tgaataaaat gggcgctcaa
      961 gtctatggag ctggtactga aacaatgcgt attgaaggcg tggatcattt acatgcagtc
     1021 aaccactcaa tcgtacaaga tcgtatcgaa gcaggtacct ttatggttgc tgctgcaatg
     1081 acacaaggca atgtgctgat tgcagatgct atctctgaac ataaccgccc attgatttct
     1141 aaattgatcg aaatgggtgc agaaatcatt gaagaagaag gcggagtccg tgtcatcgga
     1201 ccaaaacata tcttacctac agatgtaaaa acgatgcctc atcctggttt ccctacagat
     1261 atgcaagctc aaatgacagc tatccaatta gttgcagaag gtacaagtgt agtgactgaa
     1321 acagtgtttg aaaatcgttt ccaacattta gaagagatgc gccgtatgaa cgctcatgtc
     1381 aaaatcgacg gaaatgtagc aatcatggat ggaaaccatg aattgcaagg tgcagaagta
     1441 tatgcaacag acttgcgggc agcagcagca ttagttttag ctggcttgaa agcaaatgga
     1501 atcacacgtg tgcgtaactt gaattatcta gaccgtggat attataattt ccatatcaaa
     1561 ctacagcaat taggtgcaga cgttgaacgt gtagacatgg atcaaacttc tgctgaaaaa
     1621 acagcacaaa cgattgctta aggaagtggc tgaatgagta accaacgata tatcttatta
     1681 actttgataa agattttaat ggtgatctta ttgcttatcc ttctttttgt ggccggaacg
     1741 atgatcggat atggtgtcat cggtggcggc aatccattta aagtatttca gccaagtttg
     1801 tggattcata ttcgagactt ttttcattga taaataagaa tcacagtggt tctagtcgat
     1861 atgaagtgag ccatgaagca gaacatgata aaagaaaaca gaagggaacg ttgacgttag
     1921 tcgcgatccc tttttttagt aaaagaaaag aaggaatcta attgatgaag aagatattga
     1981 tttatttagt tagaggatat cagcgcttta tttcgcctgc atttccacct agctgccgtt
     2041 attacccaac ctgttccaat tatatgataa aagcaattca aacccatggc gcgctaaaag
     2101 ggacggtaat ggggactgca cggatcttac gctgtcatcc ttttgtcaaa ggcggtatcg
     2161 attatgtacc attgacattc cgattaacgc gaaataagaa agataaaaaa gaaccagatt
     2221 ataaaaaggt tgtgaaaaat acgggtagca ctgagcgata aagaaacaat ttggaaaata
     2281 gcttcttata ttttgcaaaa ttgtgagtta ttgccgaagt gttggatttt gaacaccgtc
     2341 aaaaaaaagg ttgtgaaaaa tacggctagc actgagcgat aaagaaaaag aggaaattta
     2401 ttgtcaaagt aacaactccg caactactta tatgggggac aaaaaatgat cgaagaacta
     2461 aacaagaaag atttaccatg gtccttgtta ttagatgcag atcctgataa agaaaaagtt
     2521 caagtatatg tttccagagg gagtggctgg atatggaaag aaaaagaaaa aacaataggt
     2581 gtactgatct atgtggcaag ggaaaaagaa tacgaaatcg tcaatgtagc agtaacccca
     2641 acgtatcaag gaaaggggat tggcggaaaa ttacttgaga cagcttttca gaagctttca
     2701 caactcgctt ccagtcaaac tcagatcatc atacgaaccg gcagtactag cagtgcggcg
     2761 ttacatctct atcaaaaaat gggctatgtg gagattggtc gagtcaaaga ctattttatc
     2821 catcattact ctgagcccat ttttgaaaat ggagatcaat taagggatca agtgatttta
     2881 gcaattcact tacctaaaaa tagctttaac taataaaatt tcaattcaag aaaatgcttt
     2941 caaatgctaa atagatacat tacaatagct ttagcaaaca aaaaggagaa tatttttatg
     3001 ggaatattag tttttgattt tggtggatcg gctgtcaaat atggttgttg ggatggcaaa
     3061 gagattaaag gtaagggaca atttgcaacg cctgaatctt gggaagaaat gaaagcgcaa
     3121 ttatttcaag tttataaaaa aatactcgtt tcttttgaag tcgaaggaat tggaatcagc
     3181 tctcctggag tagtgaataa tgagaaacag gtgattgagg gaatcagtgc gattccttat
     3241 attcatggat ttaatatttt ccgagatttg gaaactctgt ttcaacttcc agtaacgatt
     3301 gaaaatgacg ccaactgtgc cggaatggct gaattttatg aaggtgctgc ccgcgattat
     3361 caagatgttg cttttgttgt ggcaggaact gggattggcg gggcgttgtt cacgaaagga
     3421 caaatcaata aaggggcaca tctttacggt ggagaatttg ggttgatgtt cttaaatggc
     3481 gataagacat tcagtaaact tggtacagcg gtacagatgg cttggcgtta ttgtgaacgc
     3541 aaaggtctcg ataaaaatgc ttataccgga aaagatgtct ttgaattagc agaaactgga
     3601 gatgcaatag caaaagaaga agttgagagt ttttatacgt atcttacaaa gggattattc
     3661 agtattcaat tttcgtttga tccagaagtg atcgtattag gcggcggggt ttctgcaaaa
     3721 gaagggctca ttgatgagat caaaagccga atgaagaagc tgacagaaca attcgatctg
     3781 catgatttcg aaccgcagat tttattatgt gaatacagaa acgatgctaa tcttgtaggt
     3841 gcagcagcaa actatctcgc tgtacgtcaa ggggagaatt aagagcttta gagaaggtgc
     3901 agcttgcata aaagaagtga atgaaatgaa taggagattt ttacctaagg aatgttttat
     3961 ttgctgcagt atgataaaat atctgagagg tgaaaatgat gtcaaaaaaa aataaagata
     4021 ttgaagtgcg tatagaagaa gtgaaaaaag atatcaaagg taagaattat gaagtcactc
     4081 agttatttat agggaaaaaa atgattggtg agatcttagc ctatggacca aaagaatatg
     4141 aaattttctt tggtgaagaa gattttggaa aagaaaaaag tcttgaaaat gcgatagaaa
     4201 cagtgattcg acactggaat ctgcacgaat aaaaaaatat caaaaaaact ttcaataaag
     4261 ggttgcaaaa atctggaaaa ttaggtatta taactaagtc aggttgatac aaacctttcg
     4321 gaggagtagc gaagtggcta aacgcgacgg actgtaaatc cgttccttag ggttcagtgg
     4381 ttcgaatcca ctctcctcca tttcattggg gtatagccaa gcggtaaggc aagggacttt
     4441 gactccctca tgcgttggtt cgaatccagc taccccagta taccacaatc gtaagctctc
     4501 gttagttcga gagctctttt ttttgctttg ctaccttgtt ccttgaagat tctgctgatt
     4561 taagatagag ttaagttaac tacaagaatt ggagggatcc acatgaaatg gaaagaaaaa
     4621 gtagacgaag cagctgaaaa gttatatgat ttggtcaaat cagaaaaata caatattgaa
     4681 gtgaatattc cgaaaaaagg cggaaaagcg gtccgggtaa aatcaaaacg cccaacgaat
     4741 catacaaaaa aatggatggc taaaaaccgc taataataac aaaagatcca taagtcggca
     4801 ttttcatcga ttttagggtc ttttgttatt ttctaggttg gacatatcta aaaaattgtt
     4861 tctgacaata ggagtaggaa gaacgatgtg tgtctaaatc ctctgttgat ggatagaaac
     4921 ctttatcttt ttcagttgtg aatcaggtat ataccagtta taatagaaga aacgaaagga
     4981 cgggtgtacg ttatgtcgaa tcaattacct gtatatatac agattcatga ccaattaaaa
     5041 aatgaaatcg aacaaggtat atggaaagtc ggagaccgtc ttccttcaga aagagaatta
     5101 gcagtgaaat tcaatgtcag ccgtatgacg ttgagacaag caattcaaaa cttggcagat
     5161 gaaggaatct tagaaagaaa gatcggttcg ggtacatatg ttgcacgaga aaaagtacaa
     5221 gaaaaaatgt ctggtgcaac aagttttaca gaaattatgg aatcacaaaa tcgagtaccg
     5281 tctagtcgga caatctctta tttcttgact tcgccaagct caagtgaaat ggaaaaacta
     5341 aacttgagta aagatgacac gatcttgcga atggaacgta ttcgtttcgc agatgatgtc
     5401 cctatttgtt ttgaagttgc cagtgtgcca caaaagctga tcgatggcta cagcaaagca
     5461 gagattactg aatcgttgta tcgcacgcta gaagaaaaag gcgggaaaaa gatcggtcca
     5521 gcgaatcaaa cgattactgc tgtgttggct tctgagaaaa ttgctgacta tttagaggta
     5581 aaacgaggag atgcagttct tcgtttgcgc cagatttctt attttgaaga cggtacgccg
     5641 tttgagtatg tgcgtacgca atacgtagga aatcgttttg agttttattt ggaaaaataa
     5701 aaaacaggtg atccctgatt ccgtattaga aataaaagag aatcagaatc acctgttttt
     5761 atatatgatc agttaccagt tgctcgtgat aagaaacagg accctcttca aattggactt
     5821 gtccgttcag cagctcaatg atggattctt taaagtggtc agcacttttt tcatctacag
     5881 cacagatgac gctaacttga tcagtatatt ggatttcttt tataaaaaaa tgttggagtt
     5941 caagataatt ctgtaatttt cctagttgcg gatacgtgat catcacagag atctcctgtt
     6001 gcaaggtacc ttctacgatt ccaatgtcag acaatgcttg agagaccgca tgagtgtatg
     6061 ctcgtattaa tccgcctgtg ccgagtttcg ttcctccaaa atatcgagtg actactgcag
     6121 ctacattgat cagttgattt tttttgagga cttccaacat cggtatgccc gccgttccac
     6181 ttggttctcc atcatcgctg cttcgctgga tttcattttt ttctccaatg atgaaggcgc
     6241 tgcagttatg gttcgctttc caatgttctt tttttatttc ttgaatgaaa ctttttgctt
     6301 cgtcctctga ttctattcgt ctaagagaac agataaagcg tgattttttg atctcgatct
     6361 cactttgtcc attttctttt attgtacgat agcgctcgat catcttgtca cctctttttt
     6421 cattttcttt attcagtata aataaagttc taggttgttt caacactgta acggttgcgc
     6481 aattgctgta gtatgcgata ataaagacaa aggcaggtgg atctggggat gcaggaacga
     6541 atgaaaaagt acgatgcaat tacgcattat ttgaagaaca atggcggctc tcaagtaacg
     6601 ttgaccttta cccaatttga cgaactgctg tttcccagta acggacttcc caaaacagca
     6661 agggaaagta cagattggtg ggcaaatgat tataaacacc ctgaaaaagg tgcctatggc
     6721 tggataaatg caggctatga agtagttgtc atcaatttgg acaaagaata tgtcgtgttc
     6781 aataaattag tgaaatctag ttggctattt gattaaataa accgcgttta gaaaacgttg
     6841 acaaatttct ttctttttat ataatagagt aacagacgcg aagaaaacgt ttgcgtagtt
     6901 tgttttaaaa aggagagaaa agtatggcta aaaaaacaat catgcttgta tgttcggctg
     6961 gtatgagcac aagtttacta gtaacaaaaa tgcaaaaagc tgctgaagca aaaggaatcg
     7021 actctgatat tttcgctgtg tccgcttctg acgcagataa caatcttgca aataaagacg
     7081 tggatgtgtt gttattaggc ccacaagttc gctttatgaa agctgatttt gagaaacgtc
     7141 tggaaccaaa aggcattcct ttagatgtca tcaatatggc tgattatggt atgatgaatg
     7201 gcgaaaaagt gttagatcaa gcaatcacgc tcataggtga ataaatagaa cagtataact
     7261 atcgaacaag cgatccaaaa aatagatcgc ttgttttttc gtatattttt ttagggcgaa
     7321 taatatgaat catttttggg gtagaaggct tctgacgaga gatattaaag caatagactg
     7381 tgaggaggta aatttaccaa attatgagaa gatcgcagca gtggaacgtt tgagcaactg
     7441 gattctttgt catcgctgtg gaatgagaac gtccgtcatt gatggacagc ttgcagatta
     7501 cggttatttt tgtacacatt gtctatattt agggcgatgc gacactcaac aagatttata
     7561 tttgtttgat cagcctgaag caaaacctcg agaagtcgtc tttttttgga caggacagct
     7621 tactgaaaaa caaacggaaa tctcaaagag aatactttgc cattcagaaa aaagacatca
     7681 tcttatttgg gctgtaacag gtgcagggaa aacagagatg ctttatccta ttttagtgga
     7741 aactttaaaa gctggaggaa gagtagcgat ttgtacaccg cgagtcgatg tttgtaatga
     7801 attgttttta cgctatcgtt atgtttttcc agaagaaaaa atcacgttgc tccatgggaa
     7861 tacagcaacg gcttatacct ttacttcttt tgttatctgt acgattcatc agctataccg
     7921 tttttatcgg tcctttgatt taatagtgat cgatgagatc gatgcgtttc cttataacgg
     7981 ggatgcagga ttagctcatg ctgtgcaaac agcgattaaa ccagacggac gattcatcta
     8041 cttaagtgcc acacctgata aaaagttact aaaggagatc aagcagacgt ttgacatcca
     8101 caaacttgct ttacgctttc atcggcgttt gcttcctgaa cccgtcttgg ttttcatgaa
     8161 taattggcga caaaaatgca agaacaagaa gaagataaaa tctttggtgc ggatcattca
     8221 tcaattaatt aaagaaaacc atatcctttt attttgtcca agtatcacga tgatgcatcg
     8281 tttgaaagcc acacttcaag aggctcttcc gagatatgag atcacagatg tttcttcaag
     8341 agacaatcag cgggcggaaa aagtccagaa aatgcgagaa gcaaaatatg acatactgtt
     8401 aaccacgatg atccttgaac gtggagtcac atttgaaaga atatccgtta tcgttttagg
     8461 agcggatcat ccggttttta ctaaatcttc tttggtacaa atcgcaggaa gagccgatcg
     8521 taaagggcct tatacgaaca gtcaagtata ttttttctat gaagaaaaaa ctgcagctat
     8581 tcgaaaagct tgtcaggaaa tcaaagaaat gaatgaagaa gggaagaaga tactgtgagg
     8641 tgcagttgct gcagtaaaga tatcgtccaa aatttaacat tcagcgagat tattggtgtt
     8701 tcgaaataca gattacctca gacttgttca gtttgtcgcg cacaatttca tttattggaa
     8761 acaagaggct gttctggctg tcaaaaacca atccatttca atctagagag tacgaaaaag
     8821 aaagtaaatt ctatccccgt ttactgcagt gactgtcaag aatggaagaa gaaatatccg
     8881 acctatgatt ttcagcaccg tgcctttttt gtgtacgatg aaaatatgaa ggcctggctc
     8941 aatcaataca agtttatggg agatatccga ctagcaggca cctttgcata taaatggacg
     9001 cagataaaaa aagaataccc gacatacatc tattgtccaa tacctttatc tgaaaaaagg
     9061 cgaacagaaa gaggattcaa tcaagtaagc gagatgttga aagcagcaag aattcctttt
     9121 cagatgctac tcaaaagaga gaagcatctg ctggctcaag caaaaaacac gcgaaaggaa
     9181 agattggaga tgcctcaacc ttttgaactt gcagtcacct tttctaagat acaaaataaa
     9241 aaaatattga ttattgatga tgtctataca acaggacaga ctcttttcca cgcagcagat
     9301 tgtttgcaga tcgcacagcc gaaaatcatc cgaactattt ctttggcgag atgaattaga
     9361 tgtggaaatt tgatagcgat ttaatttgca aataacctag agttcgatat aatagaatta
     9421 agaagaggga aagagtggtc cttctccttc ttgattgtgg tagacgaaag gggtaatttt
     9481 tatgtttaga tataatgtac gcggcgaaaa tatcgaagta actgaagcga ttcgcgacta
     9541 cgttgagaaa aaagtaggca aactagaacg ctatttcagt gacgcacccg atgcaacggc
     9601 gcatgtgaac ttaaaagttt atacagaaaa aacagcaaaa gtggaagtaa cgatcccatt
     9661 accttatctt gtattacgtg ccgaagaaac ttctccagat ttatacgcaa gtatcgattt
     9721 ggttgttgac aaattggaac gccaaatccg caaattcaaa acaaaaatta atcgtaaatc
     9781 ccgtgaaacg ggcatgaata ctgcaaatgc tgctgcttta ttcaatgaag aggacgaaaa
     9841 cgaaaatgat tctgaattag atatcgtacg tacgaaacgc ctttcattga aaccaatgga
     9901 tagtgaagaa gcagtcttgc aaatgaacat gctgggacat aacttcttta tttttgaaga
     9961 tgcagaaaca aatggtacaa gtatcgttta ccgccgtaaa gatggaaaat acggactgat
    10021 tgaaacagac tgatcagcct tacttaaata acaacgaaaa cttgcagcag ctactgacag
    10081 ctgccgcaag tttttttgag tttcctcctc ttttagatga aatatgaata aattatgaag
    10141 ttc
//
LOCUS       DAKBVG010000096         9913 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000096 DAKBVG010000000
VERSION     DAKBVG010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9913
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.096"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(10..555)
                     /locus_tag="OUD09_001662"
     CDS             complement(10..555)
                     /locus_tag="OUD09_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCT9692933.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(567..1259)
                     /locus_tag="OUD09_001663"
     CDS             complement(567..1259)
                     /locus_tag="OUD09_001663"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCT9692934.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYKPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1278..1562)
                     /locus_tag="OUD09_001664"
     CDS             complement(1278..1562)
                     /locus_tag="OUD09_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9692935.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1575..2138)
                     /locus_tag="OUD09_001665"
     CDS             complement(1575..2138)
                     /locus_tag="OUD09_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HCT9692936.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2365..3015
                     /locus_tag="OUD09_001666"
     CDS             2365..3015
                     /locus_tag="OUD09_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HCT9692937.1"
                     /translation="MIKRNWPWLLALVLLFLFGFSFVLGLLLIGVVLVLWGLFGSRRK
                     KGKQEEIPALTNELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAKLKAI
                     DLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIKNKQT
                     YEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQKD"
     gene            3139..4335
                     /locus_tag="OUD09_001667"
     CDS             3139..4335
                     /locus_tag="OUD09_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HCT9692938.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4436..5812)
                     /locus_tag="OUD09_001668"
     CDS             complement(4436..5812)
                     /locus_tag="OUD09_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCT9692939.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSFSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIISILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5849..6715)
                     /locus_tag="OUD09_001669"
     CDS             complement(5849..6715)
                     /locus_tag="OUD09_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HCT9692940.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6804..7829)
                     /locus_tag="OUD09_001670"
     CDS             complement(6804..7829)
                     /locus_tag="OUD09_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCT9692941.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYRLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7847..8521)
                     /locus_tag="OUD09_001671"
     CDS             complement(7847..8521)
                     /locus_tag="OUD09_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCT9692942.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKP
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8702..9598)
                     /locus_tag="OUD09_001672"
     CDS             complement(8702..9598)
                     /locus_tag="OUD09_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCT9692943.1"
                     /translation="MKKRTGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     RNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYWKFKRQKQLRKNNRS"
     gene            complement(9672..9797)
                     /locus_tag="OUD09_001673"
     CDS             complement(9672..9797)
                     /locus_tag="OUD09_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCT9692944.1"
                     /translation="MKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKFKMKSEV"
BASE COUNT         3011 a         2146 c         1729 g         3027 t
ORIGIN      
        1 tttgtttttt tataaaattt ctgcgcctag tgtggattca aaatgtctca atgcccattc
       61 atgtccaacg ggatcgaaac ttgccacggc atctttgaaa atatggagtt tgtatcctag
      121 attatacgca tcgactgctg tatgcaacac acagatatct gtacatacgc cggtcaaata
      181 aatatctgtg atctggcgtt ctcgtaaacg gatgtctaga tctgttccac taaaagcgga
      241 ataatgccgt ttgtctatcc aataaacatt ttcctcttgc tcatagtctt ggtataaact
      301 ttttaaagag ccgtacaatt cccgtccacc cgttcctatc acattatgcg gaggaaataa
      361 gcggttctcc ggatgaaagg aatcttttgg atcatgcgca tcaatggcaa aaacgacaaa
      421 atccttttga tcaataaact tctttgtatg agcagtcagc tttttttcaa ttacttgtcc
      481 tgctgtccca gtagtcagct ttccatcctc tgcaacaaaa tcatatgtgt agtcaatcga
      541 gatcaatgcg ttcatcttag tcctccttat acgagatgtt tgacaaattc gatcaccatt
      601 tctgctgatc gtttgcctgc gtcttcgata aattcgtcaa agctttgtgt cgccgagtga
      661 tctgctgtgt cgctcattgc acgtacgatc aaaaatggga tattgaactg acgagctgtt
      721 tgtccaacag cggctccttc catttcgcaa gctaatgctt cagggaaatt cgttaagatt
      781 tctttgattt tatctggcga gtcgacaaat gtatcacctg taacgatcaa tccttctttt
      841 gcgttcaaat tcgttgcttt tgctgcttta accatttcac tgcgcaaata tgtgcttgct
      901 tcataatata gaggcattcc tggtaattgt cctggtttgt agccaaatcc tgttgcatct
      961 gcatcaaaat aagctacttt gtctgatata acgatgtcac ctacttgtaa gccttcaccg
     1021 attccgccag ctgaacctgt attgatcacc atgttcacgc cgtattgttg gatcaacaga
     1081 cttgtggtaa tagatgctaa gacttttcct ataccggaac gtacgacgat cacttcgtgg
     1141 ttacctaatg aacctgaaat aaataaagcg cctgcgcgtt cccatgataa tggttcgctt
     1201 aggttctctc tcaaaatttt tacttcttct tccattgcgc cgataatacc gattttcatc
     1261 tttttaattc cttctttcta tataaatgcg attgctaaaa aaaccacaca taacattagg
     1321 ataacaataa taagagattt catcaaaaaa gagttccatt tggtcgtctt ttcccgttcg
     1381 ctgatccttg ttttcgtgat tggtttattc tttttgcttt ctttacgata aaagctggca
     1441 atcttttttt cttcttgctg ataagctgtt tctgcttttc gctgtttttt taatgactcg
     1501 cttgcttgtg cttgttgtcg cttgcgaagt tcgcttcgcg taataagcgg tcctttggcc
     1561 ataagccgat ccctctactt tcctttggtc aatagttccc agtattgaat cgcataaatc
     1621 gtctttgcat cacaaatcaa ctgatctttc atcgcttgtt gcgcctcttc aagagttaga
     1681 tgatacaact ccagtacttc gtcttcatct tgtgctaaag gattctcgac cttctctacc
     1741 ccttgtgcat gataaatatg aaggacttca ttagcaaaac ctggtgaaag atacatcgaa
     1801 gtaagatggc tcagcgactt cgcacgatag ccagtttctt cttctaattc tctagctgct
     1861 gtcatttcgg gattttgtcc ttctcctgga tcgatttttc cagctggaat ctccaaaatc
     1921 actttttcta aaggttttcg aaattgcttg acaagaagca gacgatcttg ttcgtcaaaa
     1981 gcaatgatcc caacaccgcc aggatgaaat accagttctc tcttcgaagt tcctccatct
     2041 ggcaaacgaa cttcgtctac agcaacatca ataatttttc cgctatagat ttctttgcga
     2101 ctgattgttt tttcttcaaa ttgctcattg ttcagcatat cttctcctgc tttctttgta
     2161 atcataactg ctttttttca tttttcaatt tttctcaaaa actccttatt agtttaccat
     2221 agataaacct tagattttct ctaaatatag aaaatccttc tttagtccga tactctttct
     2281 agtagtccgt atgctaaaat aagtatgtaa tcgaataacg aaaagaattc aatactcaac
     2341 gataaatgac atggagggtt tattatgata aaacgaaatt ggccgtggct tctagcattg
     2401 gtcttgctat ttctctttgg tttttctttt gttctaggac ttttattgat aggtgtagtt
     2461 ttagttttgt ggggactctt tggttcgcgc aggaaaaagg gaaaacaaga agaaattcct
     2521 gcactgacta acgaacttga aactcactat gaaaaaagcg gtatgacagc gaatgaaatt
     2581 tcattttttc gacaaacaat gaatcaaaca aaaaatgaga tcaatcaatt gcaaatgaat
     2641 atgcagcaga cagccaaact taaagcaatc gatctccgcc atgatccagt caaagcagca
     2701 aaagctttat tcaaagaatt ggtgaaagaa ccaaaccgac tgcatgaagc aagtcagttt
     2761 ctttatacgc atctgccgaa tctagttgat ttgacaaata agtataatga gatcaatgac
     2821 catgagatca aaaacaaaca gacctatgaa aaactagaag aaagcgcgca gatcatcgac
     2881 cagctgtcag ctttaatcgc acaagattat caaaaattcg ttgctgagga tctagatgat
     2941 atcgacgtag agatatctgt tgcgaaacag agcttaaagc gtgataataa atatgaacca
     3001 gaacaaaagg attaactctt ttgttcataa aagaagactc ggaacattca gatgatgtct
     3061 ggctgtttcg agtacttttt ctaaagtaga gacattcatg taagaatcga gaacagattc
     3121 aacaaggagg aattttttat ggatcataaa ccgactgaaa caaaagatgt tacaccagtt
     3181 aacgatacac ttgaagattt attgaacaac ccgttttcaa ctccaacaga tacgctgacc
     3241 acttcacaac aagcggagat tgatcagctc caagagcaac agacagctgc tcgtttaatt
     3301 gacaaactac cggcggaacg ccaagaacaa gctaaacagc tagcagcaaa aatcgatgct
     3361 aatgatgcac agtctgtcat cagctacgga tcggctgccc aagcaaaatt aagtgaattc
     3421 tcacaatcga tgttgaatca tgtgcaagca caagatattg gtcctgtagg agattcctta
     3481 actgaactga tgtatcgtct ccaagaagcc aatcctgacg aactgcgtgc cggagaaggg
     3541 aacatcttcc agcgtgtctt cgggaaggtc aaacaatcga tctatgaagt gacagcaaaa
     3601 tatcaaaaga ttggtgcaca aattgacaag atttcagtga aattagataa agaaaaagac
     3661 ggtctgctaa aagataatct gatgttagaa cagctttacc agaaaaacaa agactacttt
     3721 gatgcactga atatttatat cgctgctgga gaattgaaaa tggaagaact tcaaacaacg
     3781 atcattccag aagcgatgaa aagagcagaa gaaactggcg atcagatgga tgttcagatt
     3841 gccaatgatt acacgcaatt cctagatcgt ctggataaac gtacgcatga tcttcgctta
     3901 gctagacaga tcacgatcca gcaagcacca caaatccgtt tgatccaaaa cacaaatcag
     3961 gccttggctg aaaagatcca agcatctatc gctacagcca ttccgctgtg gaaaaaccaa
     4021 gtcgtgatcg cattgacctt gcttcgtcaa aaagatgcag taactgctca acgccaagtc
     4081 tctgaaacaa caaacgactt attgaagaaa aattcagaaa tgctgaaaat ctcagccatc
     4141 gaaacagcga aagaaaatga acgtggaatc gtggatatcg agacccttca aactactcaa
     4201 aatgatttga tcgaaacaat ccaagagaca ttgcgtatcc aaaaagaagg aaaagaaaaa
     4261 cgtcgtcatg cagaagtaga gcttggacac atggaagaag atctgaaaca aaaactgttg
     4321 gatttaaccc aatagatcac ctttcgcttg ttactaaaac aaacgcccaa caacagttta
     4381 ttacaactgt tgttgggcgt ttttcttatc tttcttctaa cttctccatt ggagtctatt
     4441 tctcattcat cttgctgtgt ccatagccat agccaaaata aatgatcagt ccaataacca
     4501 ttacgatacc gaacacgatc catgtcacag tttgcagacg aagcatcaat acaatacaag
     4561 tcaagatgga aatgattggc aatagcggaa cgaatggcac tttgaattct ccagcttttg
     4621 gtttgccaaa catttttctc aactttagta agcctagact aacaagcatc aaataggcga
     4681 gcgccatgat attcgttagt tccgctaata cattcaatgg gaaaaatccg gcaaagaata
     4741 cagctgccac accagcggca taagtcgctt ttttcggtgt acgatgcttt tgatcgattt
     4801 ctgtcataaa cttaggaagt aagccatcct tgctgacagc aaacagtaaa cgagcaagtg
     4861 aatacatcat cgagattgtt accgtcagta gcgttaaaat ggcccctaca gcgatcaccg
     4921 cgcccactgt tccatgtcct acatacctca ttgcaaaagc cactgggtca ttgactccta
     4981 gttcagtata cggaacgatt cctgtcaaga tcagcgtcac gatgatgtaa aggacagtag
     5041 caatcagtat cgaaccaatg attcctcttg gcacatcttt ttgcgggttt ttgacttcct
     5101 cggctgccat tgcaacggca tcaaatccca agaaagcaaa aaacacaaga gctgcaccat
     5161 ctagcacacc ggaaaaacca aaaggcatga atggctgcca attatcaggt ttaacgaaaa
     5221 atattcctac aatgataaac aacgcgatga taccaaattt gacatatacc atggcattat
     5281 tcaaacgtaa tgctttcttg gcttcaagcg acacgatgta agtgacgaat atcactacga
     5341 gcaccgcaat caagtcaatg tatgtaccat tttccggatt gtacccgcca gttaatgctt
     5401 gtggaagttc ggcccctaaa cttttcaaaa aaccttgcat atagccagac catccggaag
     5461 caacagagga aaccgctagt aaaaattccc cgatcaacaa ccaccctgtc agccaggcag
     5521 caaattcacc aaatacaaca tacagatagg catatggccc accaatcact ggcactcgcg
     5581 aagcaaattc tgcgaaactt agtccagaca aaatgacaac gattgcagct aagacaaatg
     5641 aaaatgacag tgctggacca gcattttgat tggccgcaac ccctgtcacc acaaatattc
     5701 ccgtaccgat gatcgcacca attccaagca tgatcaaatc agacgttttt agttcttttt
     5761 tcatctcact cgtatgattg agattgatct ctttcttacg aaacaaatcc attgaagttc
     5821 ctccccattt taggttggaa aacagcgatt aaactgtttt catataccct aaaaggatac
     5881 cacctaaaat gactaaaagg caaccaacaa taacataaat gagctctttt ttcgtttttc
     5941 tttcgccaag gatgaaaatc ccacctaaag tagagataat gatccccatt tgtgaaagtg
     6001 agaagccaac agctaaacca agtgatttga ctgttagaag catgcagatg tttcctaagc
     6061 cccataataa tccacagatc atgtttttcc acacaaactg atcgacttta actttacgga
     6121 aagcaaagaa gcttgcgcct aagatcattc cgatactttg aggtaagata attgacatcg
     6181 catccaaatt cgcccagttg acgatgatgg tatatacacc ataaccaatc gtagagaaga
     6241 tcaatgctct aaaaccctta ccaaaatcaa gcattttatt atctgtatct atttttcctt
     6301 cgtcatcttg tcgggcagtc aaatacgcac cgcttacaag aagagctaaa gcaataatac
     6361 cgtatacata atctttcgtt tgtgtccatt catggaagaa aacggctccc gcaatcgtat
     6421 tcaaaattaa ttgaaaacca gtagacaatg gtaaccctac agaaacaccc atgtacttca
     6481 ttccatggaa ctgtccattt tgtccaacac tccaagctaa gccggataaa aatccgatca
     6541 agaaaatcca aggggtgatt acaggactaa caacgaaaaa gacaactaaa gaaaacagaa
     6601 atgcaccgat cgtcattcct agtgtttgtt gattggcatc gccaccaatt ttaccactta
     6661 ctaaaccaat actaccccat gccaacatag ggactaatgc tattaaaatt tccataacca
     6721 atctcctttt atttcacatg ataagaaact tatcagcgaa aacgttaaca acacaagcac
     6781 tttttttcgt ttctaacgat tttctaacgt ttttcattat aaaaatttaa gattgggaaa
     6841 aataaagtca ttgttgtccc ttttccttca gttgattcag ccgtcaaatg gatacctaat
     6901 ttttcggcca gattttttgc taaataaagt cctaatcctg ttgaatgtgt tttgctaagt
     6961 cgaccatttc tcccagtaaa tcccttatca aagatccgtt tcaagtcttc cgctgggata
     7021 ccgatccctg aatctttaac tgaaaggctg actccttgtg cttccttttc tatgctcaca
     7081 atgatgtttc cgtgatccgg cgtatatttg atggcattac taagcaattg gttgaagata
     7141 aaagcgatcc atttagcatc ggtcaaaaca gtatgatcct caccaacaat ctcgtagcga
     7201 atattttttt ggataaaata atttgcctga ctgcgtatca caggctgaat gattcctttt
     7261 agcctatatt cttggatgag gtagtcccgc gaaaaactgt ctagtcttgc ataatacaaa
     7321 acttgttcta cgtattcatc gatttttgat aattcatttt ctagtaatgt atacttttga
     7381 tcgtcaatat cgaactcgat cgaacgaact agcaatttag aagcggaaag tggcactttg
     7441 atttcatgga cccatgagtc aatataatct ttttggtctt gttgattact gattgctgat
     7501 tgcattactt cttggtgttc acggatcagc tgattgatat attcttctac cagtttttct
     7561 tcttctgttc gtccaccgct caaatagttt tgcaaagggg aagttttttg tatgtcggct
     7621 aattttcccc accatcttct cttgctcaag taaccaccca gcagataaaa aatcagaaag
     7681 acgccctcaa tcaaagctaa ataagcaatt gtgctcaaat caactttgat gtttggcgcc
     7741 agccacatca caaagatcgt caatgcaata aaaaacagcc atcccaagat caatgtccac
     7801 tgatctttca aatatttaaa aaaattcatc ttaaccgctc atcctttcag ggaacgatat
     7861 atccctgtcc tacttttgtt tcgatatatc cttggatacc tgcctgttcg atttttcggc
     7921 gtaaccgatt gatgttgact gtcaatgtat tgtcatctac aaatcgttcg tcatcccaca
     7981 gtgctcgcag caatttttcc cgacttaaga tctttccatt gtgcttcatc aaaataaata
     8041 atagacgata ttcatttttg ctcaaatcaa tgatctctcc gttgatttcc atacttccgt
     8101 tgtcaacatt caacgtaatg ccattgtggc tcatcatttc actagataat tctgtgtagt
     8161 tgtaagaacg gcgaagtaat gcattgattt ttgcaatcag tacatcgatt tggaaaggtt
     8221 ttgtcacaaa atcatctgcc cccatgttca tagccatgat catatccata ttcgtattcc
     8281 tgctggagat aaaaatgatc ggtactttcg atacttcacg tattttttga caccaataat
     8341 agccatccaa aacgggaaga ttaatatcta gtaatacaag ctgtggttct tctctttcaa
     8401 agtctgagaa cacttgattg aaatcggtcg ttccaaaggt ttcaaattgc catttctcaa
     8461 gttcctccat gatcaattgt cgaatgatct cttcgtcttc gactaccatg atttttgcca
     8521 taaaaaatcc ctccatcttt cttcttgcta ctacaatatc aaagaagccc ttgtgaaaca
     8581 agttggctta cctggaaaat gtcattttag caaacgttaa aaaaaactgg aaatatgcga
     8641 tttaatcgct tttgtctttt caatacagct taaatttatt atgttattct taacaattgg
     8701 tctatgatct gttatttttc cttagttgtt tttgtctttt aaatttccaa taacaccaat
     8761 aaccgaaaag cgttagcact agcaatatac ctagaaggag tataagtaat cgataaagat
     8821 ggtatttttt tgtctgttct atctttgaag agacttcttc tgtaaaggga actcgccttc
     8881 ctgtcactag taatcggtga gtgttcacgg tgtaaggtgt acaagtgata agtgtgacta
     8941 gatcctgctt ttctctgatt gctaatgtat ttgtttttgt tggaaggaca acaattttct
     9001 caacgacttc ataagcgagc atccgaccat acacttcaag atagaatcgg tcatcttgct
     9061 ccatttcttc aagatccgta aagagttttt tctcagcaat accgctatgg gcggtaatca
     9121 cggaatgtgt actctctcct ccagttggaa aagaggtgcc ttgcaggact gtcgctcctc
     9181 tatctaacaa aagatcattc gtttcatcaa ataaaggcaa gctaacattg attttcggaa
     9241 tatagattgc tccgatcata tgttgttcgt agtattcttt tcctggattt cggacatttc
     9301 taaccgcttg ctcaaaagga tctttgactt gtcccattcc tgggattact gatgtatgag
     9361 caagcgtctg atttttcttt tcatgcactg ctaattgttg ctcatgtttt tcttctttat
     9421 cgcgttcaag ctgcttttga taattctcga tccttctttg atccaaataa ttgctgagag
     9481 catctgctaa aaatggatat gtaaaaactg cagctccggc aagaaacatc actgccataa
     9541 ataattgtaa taaaagactg atttgggttt gaggtctatt acctgttctc tttttcattt
     9601 tcttcacctt cttatttaaa aaaactgcag caatcggttt tataaaggtt gctgcagctt
     9661 atttttgttg cttatacttc agacttcatt ttaaatttac ggtaccagct gtacgctcca
     9721 atcatcaaac ttattccgat tagtaagaat gccaagagcc cgttcccacc agtagcagga
     9781 agtgacccgc gtttcatatt cggtactctt tctggccctg gattttctac tcctacgatc
     9841 tgaatttctt cattatagct tccataagag atgataaatt cagtaaaggc tgcctcacct
     9901 gttggaagta cat
//
LOCUS       DAKBVG010000097         9818 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000097 DAKBVG010000000
VERSION     DAKBVG010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.097"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            117..3407
                     /locus_tag="OUD09_001674"
     CDS             117..3407
                     /locus_tag="OUD09_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HCT9692945.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEVPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESCTYT
                     NAQEGFALMVRDAVPKDHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKDLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIKLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSFLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTVSKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNESPNESPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVV
                     YVMKQRKK"
     gene            complement(3947..5854)
                     /locus_tag="OUD09_001675"
     CDS             complement(3947..5854)
                     /locus_tag="OUD09_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HCT9692946.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNEQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIQYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5984..6859
                     /locus_tag="OUD09_001676"
     CDS             5984..6859
                     /locus_tag="OUD09_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HCT9692947.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     ALIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKEWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6939..7913
                     /locus_tag="OUD09_001677"
     CDS             6939..7913
                     /locus_tag="OUD09_001677"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCT9692948.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8093..9238
                     /locus_tag="OUD09_001678"
     CDS             8093..9238
                     /locus_tag="OUD09_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCT9692949.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSCQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLEQLVRAVKK"
     gene            9375..9719
                     /locus_tag="OUD09_001679"
     CDS             9375..9719
                     /locus_tag="OUD09_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HCT9692950.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
BASE COUNT         3195 a         1809 c         2068 g         2746 t
ORIGIN      
        1 atgttttttc tttttataaa atacacttat tataattttg ttttttattg acagtaaaaa
       61 taaattaggt aatactttaa gtaaacgttt tctaatttgt tgcgggagga atacacttgt
      121 taaaaaaacg tataaaatac agtgcgctaa tgatgaatct ccttatgctc agtaccccgg
      181 ttttaatttc tgtcgttgaa gttgctgcta acgaacaaca gtctgtgaac gaagaaaatg
      241 cacacgtgaa cattgaaggg atgtcaacaa tggaggaagt accaagaact agtgaagata
      301 tggatacgga agaaggaaat gatcctatga tgaaagaatc agaaaatgaa aagacaaaaa
      361 agcaatctga agaagcaaat gaagagtgga aaggggttgt tttcggagaa agcacaagtg
      421 ctagtaggta tgccattgaa ccattagaag acggcgtgcg gctctcttcc acgaataacg
      481 gcggaaaatt ccagtcaagc ggatcagatg gaatgaccta ttattatcaa gcaatcccca
      541 aagacatcaa ctttaggatg cgggcgacga tcgaaataga atcttgcacc tatacgaatg
      601 cccaagaagg atttgcttta atggtaagag acgcagtacc aaaagatcat ttgtttgggc
      661 aggcatttta ttcaaattca tttgccatac taggcagtcg gatcgaatat gtctgggact
      721 cggatagaca agaagtggtc aacacgagcg gcagcaaata tacgatgcga cttggattgg
      781 ggacacgggc aatcacagga atatcatctg aagatccgca agctgcgcca gctcctggga
      841 gtgtctcagt ggaaacaaca cctttagaga cttcagcagg gttcttggaa aaagaaacag
      901 gttcttataa tatattcgga aacggtcatg ggaatctgtt tccggccacg aactcgatta
      961 ctaaactgga cgtagagatg aaaaaaacga atactggttt agaggcatat tattacgatc
     1021 ctgaaacagg agaagaaatg gcttctgaca tcatgtatga ctgggaaaaa ttatacgctt
     1081 cagatgaatc ggttatttat gcaggatttg ctgcagcaag gaatatgacg atcaaggtag
     1141 aaggaatcga agttgcttac tcagatcctg cgacagatcc tcctgggcaa gaaagaccta
     1201 ctcgcttgat tgatccgatt tacactgtga cctctagtaa tcattcagga aaccaagatc
     1261 atactatgtc ctttagagca aatgctgacg gattattgac aattaaaaat aaagcaacag
     1321 gagaggtgct taagaacgca aaagacttgg ctatcactgc gaatcgggaa tttgattatc
     1381 agacaaaact gatggaagga acgaacgaat ttactttcag ttttacgcca gataaaaact
     1441 atccgccaga agaatacgta gagatgacta gttatgaaac aaaagagatt cttcatacag
     1501 tggactaccg taaacctaaa tattcgacag tttatgtaac accagaaggg tcagatgcag
     1561 gaaacggaag cagacaaaat ccattatctc tgacacaagc attgagatac gcatatgctg
     1621 ggcagacaat cgtcatggcg cctggaactt attccttcga aagaggattg acgattccca
     1681 aaggcgttaa cgggacaaga gaaaacccga ttaagttgat tgcagagaaa aatccagaaa
     1741 acaagcgtcc tgtacttgat tttaaaggaa ctggaaatgg gatgacactg ttcagtcact
     1801 actggggttt acgagggttt gacattacaa atagtgcagc tatgcaaaaa ggattacaga
     1861 tttctggaaa tcataatttg atcgaagaga ttcatgctta tcggaatgga aatacaggaa
     1921 tccaaatcag cggtagctca aatgatcctt tcgaagcatg gcccgcgcat aacttagtca
     1981 agaactgtac atcttttgaa aatgccgatc caggatttga agatgccgat ggatttgcgg
     2041 ccaaactgac aataggagaa ggaaatgtat ttgacggttg tatcgcgtat cataatgcag
     2101 atgacggttg ggaccttttt gctaaaaatg aaacaggttc gatcggaaaa gtaatcatca
     2161 aaaactcagt tgcttatcga aacggctggg taccgggaat tgagggagaa ggtaacggta
     2221 atggattcaa aatgggcggt tcttttttaa cgggccctca tgaattgatc aatagtttgt
     2281 ctttcgaaaa tcttgcgaaa ggaatcgatt ccaatagcgg aacagatatt atcgtcaata
     2341 gcagtacaag ttttaataac ggttcatata atgtggctct ttatacctct tcagcaggaa
     2401 acacagacta ccatgcttct ggtatcttat ctttccgaac agagaatctt gagatgagag
     2461 agcagatccg cccattgaat caaaacgaag atcaaatcta ccactcgttg aactactatt
     2521 ggaatgaacg agagaaacaa tcagaaaata ctcttggaca taccgtatca aaagattgga
     2581 tcgagtctct aaatacatgt tcgaaagaag gtcagcagcc tgttacgcgc tatgaaaatg
     2641 gaagtatcca tgttcatgga ctgatgcaga ttaaaccggg gaatcgtcaa acagaaaagg
     2701 aaagagtgga tggtcttcca ttatctgtag gtgctatttt tgacggagaa acctcgccat
     2761 cttcggaata tccagagtat gcaatcgtac aagaaccaaa tcagacaaaa ccaaatgaac
     2821 aaaataatcc gattattata caagaagcaa atggtgtggc ggagaatgca gaaggaaata
     2881 tcattgaact gaagcctttt acagtttttg tgtcaacttt cacaccaaat caaggaacgc
     2941 ctgtctttga agatatgcgt tatttcccag aaaagatcca aatcgttgaa acacagtcaa
     3001 gtgctcaatt ttctaatcat accgctattt tttcagcacc ttcaaaagga gaatatacgc
     3061 tagaagtcac ttatcgttcg gaaatatttg atggggaaaa atggtctgct catacagaat
     3121 ctgttaccca gcaaaaagcg atcactgtgc gagaaactgc cgatgtgccc ccgggaaatg
     3181 acaaaccaga aagagaaaat ccaggaaatg aatcaccaaa tgaatcacca aatgaatcac
     3241 caaatggaaa accttctcat ccggacagga cagcaaaaga aagtgaaaaa aatggcactc
     3301 aaggactccc aaaaacggga gaatttaata acccgttgct agcattagct ggaggaattt
     3361 tgttaatcgg tgtagttgtt tatgtcatga aacagcgaaa aaaataagtg gaatttctcc
     3421 ttttatgatg taaaacaatt gactgaggaa agtaaaaata gaaggaaatg agaccaatag
     3481 atgtctcact tccttctatc tttttataga tgaggttctc cttgctgctt ttgattggct
     3541 gttcttatta cttgatgcag gacagctttt tcacaacctc tccactaaac ggtgttcaat
     3601 cagctgaccc tcggtattag ctcaacaaac ggcaaaatat gaggaactat ttttccgttt
     3661 tttctctaat actcaggtca ggacgctgat ttcacaacct ctcgataaac ggtgttcaaa
     3721 agccagtttc tcagtagtaa gtcaaattta tgcaaaaatg tggaaagcta ttttcacaaa
     3781 atttttctta ctacttgaaa ctaagcggct ttttcacaac ctctgataca tggtattcca
     3841 gaagtaactt cttcgaaaaa taagccgaaa tgtcacaaaa atttgagaag caattttccc
     3901 caattccttc ttatttcttg aagctgacca cttctggcac aacctcttac cagattcgca
     3961 ctcttttttc tggcgctaga tacatagcgt cttgttccgt gataccaaat gtttcataga
     4021 attcgtcaag gttttttggt tgcacgtttg ctcggagttt agcaggggca tggacatcga
     4081 tttgcagtaa aagttgttgg tattcttttt ttgcttttgt acgccaaatc gttgcccaat
     4141 tgacaaagaa ttcctcgata gaaagatctg attccgtttt tgctgcttcc aatgcacagc
     4201 ttagaccgcc tgcatcagca atattttccg aaacggttaa ttttccgttt acttttccgt
     4261 cagcaaaagg cagaccatcg aattcttcga tcattgcttg tgctttgttt tgaaattgtg
     4321 taagatcttc ttctgtccac cagttgttca agttgccata ttcgtcaaat aaagcgccgt
     4381 tattatcaaa tgcatgggaa atctcatgag cgataacagc tccgattcca ccataattag
     4441 cagagcttga ttgttctaga ctgtaaaatg gtgcttgaag gatcgctgcc ggaaagacga
     4501 tcacattccg aaatggatga taataagcat tgaccgtgtc agcactcatt tcccattctg
     4561 aacgatccac tggtttgttc catttgctga aacgatcttt taatgtgagt ttgctgaaaa
     4621 caagagtatt tgaaagcaaa gtaccaccct gatctgcagg tactgttttg aactggtcga
     4681 ataatgctgg gatcttatcg gggtagccta cttggatacc taagttattt aattttatga
     4741 cggctttttc ttttgttgca tccccaagcc agtcattttc ttctagacgt tttttataaa
     4801 cggcgatcat ttttttcacc atttgttcta cgtctttttt agcagcttcg ccaaaatatt
     4861 tttttccgta gtaatcgcca actacttgat cgaattgtcc agaggcaagg taatacgctg
     4921 cttttttctg aggcattgct tcgtctgttc cagacaatac gcgagaatag attccgctga
     4981 cttggcggaa ttcttctgat agataaccac taagagaacg tactgtttta atgatcatcc
     5041 agcttttgaa taacggaaaa tgagcttctg tcaggatgtc attcaaatga tcgaagtaca
     5101 ctggatcagt tacgatcact ttatctggag ttgtcccaat caaacccgtg acaagttttt
     5161 tcaagtccag tttatctgtg tattgaataa atgtatcaaa agattgcgga ttatacattt
     5221 tgctgtaatc tgcgttttct tctgcacttt ttacatgagg agcaatcaat ttatcaaatt
     5281 gcactgcttg ttccgtgatt gcttctgcct gacttttttc tttgccggca agctctaata
     5341 actgaacagc catatcaaag aaaacgccta ttagttgtgc accgttggga tggtctgatg
     5401 cataatacgt cttatctgga aggaataaag aaggcggata agcaaagaag gcgttcgttt
     5461 gcgcattctt catatctgca tctacatcca gattaaatgg caatggtaat gtatccaaca
     5521 gccattctgg aaattgctca ttcaattcgt cgaaagactg caatgattca actttttcga
     5581 ttataggttg aagtggttga gcatgcaact catttcttcg gtcataatcg tttgccagtt
     5641 gataatatgc aaggaaatgt ttcatttttt ctgaagacac tttttctgga tgggcaagca
     5701 gatcgtctgt ttctcccatc aaaagttggt cgatcccgtc aactaaatct tggaaaccgc
     5761 ctgtagctgg tttatctgca gggatttttg ctgttttttg ccattcttca ttgactgctt
     5821 catagaaatc ttgtttcaat cgttctttgt tcattcatcg atcgctcctt ttttgtttcg
     5881 tgtgcccatt atcttacaaa atgaaggaat taacaaatag aacataaaaa attgaaaatt
     5941 cgaacaatca aactgatttg tttttttcaa tccttcactt ataatgaaaa aaaggaggcg
     6001 atcccaactg aatcaagtag tggataaacc tttatatttc aaagtagata aaaaaattaa
     6061 aaaattagcc agcactcagc agctgcagtc aagaaaaagt aaattgcttt ttttagctct
     6121 ggtttttgaa gatcaaagtt atgtgattat cgatcaatca ggacatccgg tagaatattc
     6181 tccagcaaaa tatacttatc aagagggtct ttcttgtaaa aaatggaagc tgatcaatga
     6241 agagccaatc gagttatcta gatggatcaa cagaaaagaa gatattcccg ctttgatcga
     6301 agaaaaaagg tcaggaaagg aactcgcaaa ttgctgggtg ggattgccag aagaacgttt
     6361 tttgaggtat aagaaatggg ctaccccctc aggctatctt tgtggtactt acgcggcagc
     6421 agttctcctt gcctattatc aagactatcg taaggagtgg atgcttccaa atgagattcg
     6481 caagaaaaat acggctgatt cgttggtttt gacgaaggca ttgcgtagcc agatccaacc
     6541 actaggactt cctacgattc cttttcaggt tagtacaggg atttctagtt ttttaaagaa
     6601 gaatggcaat catgagcgtg cacgagcaac tcttctaggt tcatggcagc gagctacaaa
     6661 acgtatacgt gaaggcaaac ctgtgatgat tggtattttg aaagtacttg gcagtacata
     6721 tggcaatcat tgggtgacag cgtacgctta ttttgaaact gaaacaggag aacgttatta
     6781 caaagtacac gacaactggg gcgattatca taaagtcatt cctgccagtt ggagtaacgg
     6841 aacagtttca cttccttaag aatttgctga aattaacaga aaatcttgga aaaatatgtt
     6901 aaactatcga agaatagaga agttttgggg aggactacat gagtaataag tatcaggatg
     6961 acacgttgaa gatttttagt ttgaatggca accgtccatt agctgaaaaa atcgcgaaag
     7021 tgttcggaac agaattaggt aaaagcgtcg tgaagcaatt tagtgatggc gagatttcca
     7081 tcaatatcga agaaagtatt cgaggggatc atgtgtatat cgttcaatca acgaatgcac
     7141 ctgtcaatga ctactatatg gaactactga tcatgatcga tgcaatgaag cgagcaagtg
     7201 cgaaaacgat caatgtcgta ttgccttact atgggtatgc taggcaagac cgcacggcga
     7261 aacctcatga accgatcact gcgaaattga tcgctaactt gatcgaagaa gcaggggcaa
     7321 ctcgagtact gactttagat ctgcacacgg ttcaagtaca aggattcttt gatattcctg
     7381 tagataattt atttacgatg ccgttgtttg ctcattatta ccgtcaatta ggtctgacgg
     7441 gagatgacat cgtagttgtt tcgccaaaaa acagcggtgt tcagcgtgca cgtagtcttt
     7501 cagaatattt gaacagcaca ttagccatcg tagatcatgc agatgatgct tcaactgagg
     7561 gtggttatgt gatcggtgat gttcaaggaa agacatgtat catggtagat gatatcttga
     7621 acacaggagc aacgcttgca cgagcagcaa atgtattaaa agaaaacggc gcaaaagaag
     7681 tatatgcctg tgcgtcacat ggattattgt ccgatcatgc gaaaagtatt ttagacaatg
     7741 caccaatcaa agatatttgt atcactgatt ctgttttcac agaagaaaat cgtcatccag
     7801 aaaacttaac aatcgttaca tgttcagaat taatgggaga agcggtcaaa cgcatccatg
     7861 aaaatacacc gatgagtccg ttattccgtc tggaagaaaa agaattcgaa taaacaaaaa
     7921 aaagagggaa agaagttgtg acacaagtga gcagcttcaa ggagctgcga caagactttt
     7981 gtcatagctc cttttttcta tgcccatgaa acgtcctcta gacaaaaatg agctcttctt
     8041 tggtttgttc gctattttat gctagaatgt gcctagcaat aatttggaag ggatgaatga
     8101 agtgaaaact atttatctgg atcatgcggc aactaccccc atgcatccac aagtaattga
     8161 agcgatgacg gcaatcatgc aagacacgtt tggaaatcct tcaagcatac atggatttgg
     8221 tcgccacgcg catgaaaaat tggaaacagc aagacaaatc attgcagaca gtctgcaagc
     8281 aaaacctcac gagatcatct ttaacagtgg gggaacagaa ggggataata cagctatctt
     8341 agaaacagcg ttctcgtgtc aaaaagaagg cagacatcta atcactacag ctatcgaaca
     8401 tcctgctgtt cttaatacca tgaaatattt agagacaaag gggtttgacg ttacatatct
     8461 gcctgtagat gaaaaaggga atctgtcgat cgaagaggtc aaaaaagcgt tacgagaaga
     8521 tacgatcttg gtctctgtga tgtatgggaa caatgagatc ggaaatttga tgccaatcaa
     8581 agaaatcggt gaacttttag cggatcatcc agcttatttc catacagatg cggtacaagc
     8641 gtacggaaac caaacgatcc atccagaaga attaggaatc gatttattaa gtatctctgc
     8701 tcataaaatc aatgggccaa aaggcgtcgg tttcttatat aaacgagacg gagtaaatat
     8761 ccctgcattg cttttaggcg gggagcagga agaaaagcgg cgtgcaggta cagaaaattt
     8821 agcagggatc tggggtatgg caaaagcagt tgaactgctg tcacctgaag aacgtgaagc
     8881 acgtaacaac aaatataaag gattccagca attgattctt gatacattag aagcaaacga
     8941 aatcccttat caagtaaatg gtgatttaac gaataaactc cctcatgtgt tgaatcttcg
     9001 cttaattggc gaagcaaatg atttgctact gatgaagctt gatcttaatg gggtcgctat
     9061 ttctactggt tcagcttgta cagcaggtaa tatcgagcct tcacacgtat tagaagcaat
     9121 gtatggagaa gatacaccga tactaaaaga atccatccgt atcagctttg gtttgggaaa
     9181 taccgaagac gacatccagt atttccttga acaattagtt cgtgctgtca aaaaataact
     9241 agtgaagtca ttcttatatg aacagatgtc tgagagaacg ccttcatttt ttagggaact
     9301 taactgaaaa acctagtaaa aatcaggata aacttttata ataaagagaa actaatttta
     9361 tcgaggtgaa aatcatggca tttgaaacaa cagcaacggt actgggcgca ccaattagct
     9421 atcgtttgca tcctgatgca aaaagatata cattgcgcga taatggattt acagaaacaa
     9481 acggaggcaa ttatcaattg atccgaccat tagatgccac gccgcaaagc aaagaaggat
     9541 tcaaattaaa aatcactgta tcaaaagata ttcaaacatt gaaaatgtcg atcactacag
     9601 caaatggatt gaaagcagtc aatattttca aagatccaaa acagaaaatg agccaagata
     9661 aattttattt tctgatggat gggatgatta gccgtgggtt attcgaaaaa gtcgaatgat
     9721 ctgtggatat aaaaagacaa ctagttttga aacggcctat tgttagatga ctaactttgt
     9781 aaggacaacg caaattagtt gtctttttta tttttaaa
//
LOCUS       DAKBVG010000098         9727 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000098 DAKBVG010000000
VERSION     DAKBVG010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9727
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.098"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            41..1804
                     /locus_tag="OUD09_001680"
     CDS             41..1804
                     /locus_tag="OUD09_001680"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCT9692951.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            1888..2346
                     /locus_tag="OUD09_001681"
     CDS             1888..2346
                     /locus_tag="OUD09_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HCT9692952.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            2455..3138
                     /locus_tag="OUD09_001682"
     CDS             2455..3138
                     /locus_tag="OUD09_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCT9692953.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            3285..3899
                     /locus_tag="OUD09_001683"
     CDS             3285..3899
                     /locus_tag="OUD09_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HCT9692954.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            3915..4121
                     /locus_tag="OUD09_001684"
     CDS             3915..4121
                     /locus_tag="OUD09_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9692955.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            4660..5217
                     /locus_tag="OUD09_001685"
                     /pseudo
     CDS             4660..5217
                     /locus_tag="OUD09_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            5275..7314
                     /locus_tag="OUD09_001686"
     CDS             5275..7314
                     /locus_tag="OUD09_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HCT9692956.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(7423..8574)
                     /locus_tag="OUD09_001687"
     CDS             complement(7423..8574)
                     /locus_tag="OUD09_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HCT9692957.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(8728..9258)
                     /locus_tag="OUD09_001688"
     CDS             complement(8728..9258)
                     /locus_tag="OUD09_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HCT9692958.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(9357..9707)
                     /locus_tag="OUD09_001689"
     CDS             complement(9357..9707)
                     /locus_tag="OUD09_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HCT9692959.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
BASE COUNT         3486 a         1702 c         1918 g         2621 t
ORIGIN      
        1 ttttcattaa aagaagatag tacggaggag agaatgtatt atgagtacag tattacatgt
       61 attggatgca atcaatagcc ccgcagatgt caaaaaatta tctgttgagg aaatgaaaca
      121 gttggcagaa gagatgcgcc aaaaaatctt gcaaaaggac agcgatgtag gtgggcacgt
      181 tggtccgaat ctaggtgtag tagaattaac gattgcgttt cattatgtat ttgattcacc
      241 aaaggataaa atcgtttggg atgtttccca tcagtcttat ggtcataagc ttttaactgg
      301 tcgaaaaaat ggttttgaaa aaggacatta ccgtgaagtg agcggatatt ccaaccaaca
      361 tgaaagtaaa catgactatt ttacagtggg gcacacatct acatctatca gcttagcaac
      421 aggtttagct aaagcgagag atctgaagaa agaatcagga aatgtggtag catttatcgg
      481 tgacggctcc ctaagtggtg gcttagcttt tgaaggattg aacaatggtg gtgttttaca
      541 atccaatcta atcgtattag taaacgataa tgagatgtct attgatgaga atcatggcag
      601 tttatataaa aacttagctg agcttcgtca aaccaacggt aaaagcagca ataacctatt
      661 taaagcaatg ggatttgatt atcgttacgt ggataatgga aacgatctgg aaacggtgat
      721 ccgtttattt gaagaagtaa aagatattga tcatccgatt gtacttcacg tccatacgga
      781 aaaaggtcgc ggttatcagc cggcaatcga taataaaatg aaatatcatt ggcatgtacc
      841 atttgatctg tccaccgggg aggcaaagca agtaacttct ggagaaacat ttaatatgat
      901 catcatggat tatttggata agaaaatgca aacaagtaca gctcctattg ccgcagtcaa
      961 tgcagcgata ccaggcgcct ttggactgaa aacattccaa gaaaaacatc cagatcatta
     1021 ttttgacgtg gggatcgctg aacagcactc tatcactttc gcttctgctt tggcttctca
     1081 gggagtacgt ccagtcgtct tccaatcaag tactttcttg caacgggcat atgatcaatt
     1141 atcacatgat ttagcgatca acgagaatcc agtgattatt ttagtcggtg gcggaacgat
     1201 ctcagctagt gaccgaacac accaaggaac atttgacatt ccttacttat caagtatccc
     1261 gaacatcaaa tatcttgctc cagcaacaaa agaggatatg atctctatgc tagattgggc
     1321 gttcgaacaa acagcgcagc ctgtagcaat ccgaattcct acacatggtg tagaacatgg
     1381 tacagctatc gcaaatgatt atagtattcc agcctatcaa acagtaaata atggaggaaa
     1441 agtagcaatt ttagctttag gcggttttct tgaattagga aaagaaacag taaaagcttt
     1501 tagaaaaaaa ggaatcgaag caacgttgat caatccagga acgatcacag aaaatgacgt
     1561 agaaacatta gataaattaa aagaaacaca cgaattagtc atcacattag aagatggtag
     1621 tttgtcagga ggatttggcg aaaatatcag tcgttattat ggtgattctt caatgaacgt
     1681 attaaatttt ggtgcagaga aagaattcac ggatagcgtt cctgtagggc agctttatga
     1741 acgttatcat ttaacaccaa aacaaatcgt tgaagatagt ctgcgtgtct tgaatattca
     1801 ataattcatt atgctcacca tccagataat tgtttatact agtatctgga tggttttttc
     1861 attataaaaa gcaaaggggt ggatgttatg tatcagcaag tagaagaata tgtcaaaaag
     1921 ttccaagaaa aatatcctag ttatcaatca gaaaaattag gtttcttttc atttggggat
     1981 acagaaaaaa tggcagacga gttgagcgaa ctagttgtaa aaaagataaa gactggtaca
     2041 tcaagtggtt acgatctgta taaacaagag aatgagcctt taccaaaagt tggtcaatta
     2101 gacgtggtcc tgaatggaaa aaatgaacca gtttgtatca tcaaaaatat aaatgtgacc
     2161 atcttgcctt ttagtgaagt cacgaaagag catgcttgga aagaaggaga aggggatcgg
     2221 acattagctt attggcggaa agtccatgct gagtttttta aacatgctta taaagaagcg
     2281 ttgaacatag aatttacaga agagaagctg gtcgtatacg aagaatttga agtaattttc
     2341 ttttagtaag aaaggtctgc tgaaagaaat agctcattat tgtctcctga atagaaaagg
     2401 gttacatttt tatatgaagc tgtattttta tataacatta ctggaggaga tattgtggat
     2461 acactggaac aaatcaaagg cggagtcgtc gtttcttgtc aagctttgga aaacgagccg
     2521 ctacacagtt cttttattat ggggagaatg gctttggctg caaaagaagg cggagcagtt
     2581 ggtattcgag caaatacgac agaagatatc aatgaaatca agcgaatcgt tgatttaccg
     2641 gtaataggga ttatcaaaag agattacgat gggtcagaag tgtttattac tgcgacagaa
     2701 aaagaagtgg aagaactttt ggagacgcaa gcagaaatca ttgcactgga tgcaacttct
     2761 cgtaaacgtc cgacagaaaa aacaactgct gaacttgtcc aaatgattca cgaacacggt
     2821 cggttagcta tggcagatat ttcgactttt gaggaagcgc ttcaagcgca agaagatggt
     2881 tttgatcttt tgtctacgac tttagcaggc tatacagaat attcacgaaa gacgcagaca
     2941 cctgattttg atttattgaa tcaaatcata gaacaagtcg atatcccagt aattatggaa
     3001 ggacatactg actctcctga acaagtagag aaagcatatg aattaggtgc tttttcagtc
     3061 gtagttggat ctattatcac ccgtccgcaa ttgatcacta aaagatatgt cgaagccgca
     3121 agtaaagcaa ataactaaaa aacacgcacg gcacagattc ttgacaaaaa agaaaaattg
     3181 atagatagtt taaagcagat tctaagaaga tgaaaaaagc attacttttc attttgtctt
     3241 caagatctgc tttttaacga attttataga aagaaggcga aaccatgttt gaacggagtc
     3301 cttcagaatt gacagaacgt gaaaattaca agttgctgat tggttcagtc atccctagac
     3361 cagtagcagt agtaacgact aaatcagcaa aagatatagt gaatatcgca cccttcagtt
     3421 acttcaatat cgtcagttcg aatcgaccca tcttatcctt ggcgatccag agaaaacaag
     3481 atgcagtaaa agacacagca aagaatattc ttgagacaag agaagctgtt atccatatcc
     3541 ttgatgagga caacgtagaa gaagtaaata aaacagcagc caatctgcct aatgaggaaa
     3601 gcgaactgta cctgactgac ttgactttaa ctgattcagt aatcatttca gtaccagcat
     3661 tgacagaagc aaaagttcgt tttgaaacat ccttatatga acatgtcgag atacggaata
     3721 aaaataaagt aacagctgat ttatttttgc tagaagtaca gaaatatcat ttaaatgaaa
     3781 cagtctatga tgataagaca ggaaggatcg atccaagaaa attgaatgct gtaagccgtt
     3841 tggcaggaaa tgattatgca gcaataggag aaattttcac gattgcacgt cccgaataaa
     3901 aaggagcact agttatgtta aaaataagct ataaaccatc gacagattca aaagaaatga
     3961 aaaaagaata tgaaacagtg aatgattttc tgcaagggca gtacttagaa gttccgccat
     4021 tgcaagacca ttttgtcgtt actactgtta ctttagatgg taaggaaata gaaatgccag
     4081 atcaaacgat tagcggattg tttaattact ttaacaaata aatatttatt ccgaaaagtg
     4141 aaaaaacata taaaaaaaca cataaaacta aaaaattatc aaagaatcct ggtgcaactg
     4201 aaagaatttg caacgggatt ctttcaactt atttgttatt tttttctaaa aaatattaaa
     4261 gattgttaaa tttcactatt tcatttcaaa ttccattgcc taatcattag gaaaagtcta
     4321 tcatgaactc gtaaatagaa tatagtaata accttgttag gtgaggctcc tatacaaaca
     4381 taggctgttg cccagaaacg tcgaaagtcg ccaatgggta aaacagaaac tgttggatta
     4441 aggctttttc caatagctaa gaatgttttc tttacgttgt atagtgctaa aactcggacg
     4501 atgagatgat gctaggtcag aattcttgtt cgaaaaggtt tctgaccttt tctttatatt
     4561 tttgtgactg acttatttga tgagacaaac tcgctcctca cctgacaagt ggattgctgt
     4621 gtgaggagga gatgcggtga aaaaagatcg attggtggag gtcgctcgga ttcacttctg
     4681 gttgcacaat acaaccagaa aacacaacaa gcaaaactga tctctatcat gagagattca
     4741 tatgtcgata ttccaggtta cggaatgaat aagataaatg cagcttactc ttatggggga
     4801 gttgatttat taaatcaaac attaaaggaa aatttcaaat ttgaagctcc ctattatgca
     4861 agtatcacat ttcaagattt tatcgattgc gttaatgaac tgtttcctgg tggagtcaag
     4921 atcgatgcag aaaaatctct agatttagat ggcgtatata taaacaaagg agagcaagtg
     4981 atggatggta atactttact ccagtatgct cgatttcggg aagacaaaga aggagacttc
     5041 gggaggatca gaaggcagca gcaagtgatc aaagctatct ctcagcagct gaaagacgta
     5101 acttcaatat tcaagctacc caaagcggtt gggaaattac ttggtagtat ccaaacgaac
     5161 cttccagaaa gtgtgcttct ggattgtggt atggatttcc tcaaagacat atgttagaat
     5221 aaacaggtat aaatagtgaa ataaaggaat gacaagcaag agaaggagga aaaaatgaaa
     5281 agaagtgaca agcacggcaa aaatcgaaca ggcgcttata ttgccggcgc agtgatctta
     5341 atagcagctg cgggtggcgg ttatttttac taccagcact atcaagaaac ccaagcagta
     5401 gaagctggag aaaagacagt tgagcaattt gtccaagctt tgaacaaggg agattataac
     5461 aaagctgcag aaatgacatc aaaaaaggcg gcaaataaaa gtgcattatc tgaaaaagag
     5521 atcttagata aataccagaa tatatacggt gctgctgatg tcaaaggact tcagatatcg
     5581 aatctaaaag tagataaaaa agacgattct acttacagtt tttcatataa agcaaagatg
     5641 aatacctcat taggtgaatt gaaagatctt tcttataaag gaacattaga cagaaatgat
     5701 ggtcaaacca cgatcaactg gcaacccaac ttggtttttc cagaaatgga aggaaatgac
     5761 aaagtaagcc tgaccacaca agaagcagca agagggaata ttatagatcg aaatggtgaa
     5821 ccattagcaa caacaggcaa actaaaacaa ttaggagtcg ttccaagtaa acttggagat
     5881 ggaggcgaaa aaacagccaa tatcaaagcg attgcttcct ccttcgactt aacagaagat
     5941 gctatcaatc aggcgatttc acaaagctgg gtacaacccg attactttgt cccattgaaa
     6001 atcattgatg gagcaacgcc agaacttcca gctggagcta ccattcaaga agtagacggc
     6061 agatattatc ctttgggtga agcagctgct cagctgattg gttacgtggg agatatcaca
     6121 gcagaagata ttgataagaa tccagaatta agcagtaatg gaaaaatcgg tcgctcaggt
     6181 ttggagatgg cttttgataa ggatcttcgt gggacaacag gtgggaaatt aagcattaca
     6241 gatgcagacg gtgtagagaa aaaagttctg atcgaacatg aagttcaaaa tggcaaagat
     6301 atcaaattga caatcgatgc gaaggcacaa aaaatagctt ttgacagtct aggaggaaaa
     6361 gctgcatcaa ctgttgcgac aacgccaaaa accggtgatc ttcttgcgct tgctagctct
     6421 ccaagctatg atccaaacaa aatgacaaac gggatctcac aagaagatta caaagcttat
     6481 gaagaaaatc ctgaacatcc attcatcagc cgatttgcga caggttatgc tcctggatct
     6541 acgtttaaaa tgattacagc agcaatcggt ctcgacaacg gcactatcga tccaaatgaa
     6601 gtgttgacga tcaacgggct taaatggcaa aaagacagtt cttggggatc ttttaaaaca
     6661 acgcgtgtca gtagtgatgt atcacaagta gacttaaaaa ctgctttgat ctattccgat
     6721 aatatatatg cggcacaaga aacgttgaaa atgggtgaga aaaaatttcg tacaggctta
     6781 gataaattca tttttggtga agaccttgat ttgccaatta gtatgaatcc agcacaaatt
     6841 tctaatgaag atagctttaa ctcagatatc ttgctagctg atactggata tggacagggc
     6901 gaacttctaa ttactcctat ccagcaagca gcaatgtatt ctgtttttac caacaatggc
     6961 acacttgtct atcctaaatt gattgcagat aaagagacaa aagataagaa gaatgtcatc
     7021 agcgaaacag cagtacaaac gatcgtgcca gatctgagag aagttgtgca agatgtaaat
     7081 ggtacagcac attctctttc tgctttaggg attccgttgg cagcgaaaac tggtacagcg
     7141 gaaatcaaag aaaaacagga tgtaaagggg caagagaaca gtttcttgtt tgctttcaat
     7201 cttgataacc aaggttatat gatggtcagc atgttggaaa ataaagaaga tgatgattca
     7261 gcaaccaaac gagcatcgga actattgcaa tacctcaacc aaaattatca ataaaaaatt
     7321 gctttttaag tacagtaaaa ggactgtggc gttatcgtca cagtcctttt ttcaaataaa
     7381 cggttttcac gatgtctctt tcacaacttc tttttgattt actcacactt cataaacttt
     7441 ttttgtaaaa tatttgagaa caagtggaga aataagtgta cttaataaaa tcacgataac
     7501 taaaggtgaa tagtactgtg gttcgatcaa ttgactttgc tgtccaattt gtaaaatgat
     7561 caaagccatt tctccacgcg aaatcatacc agcaccgacc atcaatgaac tatgttgaga
     7621 aaaaccggca atctgagcac caaaataacc tccaagcaat ttggtcaaaa tcgctacaag
     7681 cgtcaatata gaaataaaga gtagttgttc actaaagtca gaaaaatcaa cttctaatcc
     7741 tacactgaca aaaaatactg gtataaatac tgcatatcct aaagcctcta cattgtggta
     7801 tacttcttct ttaacctttg tctgactgac agctatccca gcaaaaaaag caccaatgac
     7861 agagctaagc ccaacaagat ccgctacgta agacattcct aaacaaataa ctagcgatac
     7921 gatgataata gaagaatttg cgtacacttt ttctgcaaat gaaagtagga aaggcgcaat
     7981 ccatttgact aataagaaaa taaaaacaaa ataaattagc tgttcaatca aaacaagcgg
     8041 taaagaagca ttgctagaag cttcccccgt tagaaaagat aggctgaaac ttagaactaa
     8101 aacgactaaa atgtcatcta ccacagaagc acccaaaatt gttgatcctt cttttgtatt
     8161 cactacgttt agttctttta atacttctac tgaaatactg acagaagttg ccgcaagaat
     8221 aatcccaaag aaaatcgctt catttcccga aacttggaat gctttgcctg tcaaccagcc
     8281 aaaaacaacc gggaagatga tcccaagcaa agcaacgaac ataccaggtc ggaaatattt
     8341 tttcaaaaga gataaatcac tttccagacc agctaaaaac attaataaga tcacaccaat
     8401 ctccgaaaag tcatggacta gaatatcagg tttgacccaa ttcaatccag cttggcctag
     8461 caaaacacca acaagtaatt gtccaatcac tgctgggata cctaatctcc gcgagatatg
     8521 tgagccaatc gttgttgaaa tcaatatcag gcataaaata ccaataaatt ccattctcat
     8581 cattcctttg tataaaatat agcccgaact tagcataaca tacattattg aaaaaatagg
     8641 aaaatatact cacaaaaaaa ccaacaatcc aaaagatttt tttggattgt tggtttttat
     8701 acggtctatt ttaataacta aaaatgatta acgtatagtc atttttctta ttctgatgaa
     8761 attgataatc tcctggatgc ccatgaaaat caaaataaag ccgaatattt ggaatagaag
     8821 catcaaactt ttgaatggat tgaacaataa gacgaatccg ccaatcaaca acagaatact
     8881 actataaaca ctccaacttt tagcttttgt attcatgctg aagaacaact ggaacaagct
     8941 gttgatgata acagctaatc caagaagaat cggcaggata gaaacgattg caggtgcaaa
     9001 agcatagacg atcagagcta agatcaggaa aaaaagacca ctgaacagac cgatactcca
     9061 agaacctgtt ttccttttaa aacggtaagc ctcaaaaaga ttcaacaacc caagcaaaac
     9121 aagataggcg gtgatcagat aaccgataaa atgaaaaaca gcagaagggt ttagcacaat
     9181 cgcaatccca gaaataatat aaacagcagc tcttaataag gcgtattttt gaatattttg
     9241 tttaatcgtc tcaaacatat aagaaaacct cctttgactt attttaacgt gaactagtca
     9301 ttttaccaaa ttaaatgtta caaaatgagc gaaggagaag aatatctctg gaaaaatcaa
     9361 cttctgagga tcttttccat ttttttgcct tttgctagct catcgatcat tttatctaaa
     9421 taacgaattt ctttcatcaa gttatcctcg atgtcttcta ctcgtacacc acaaacaaca
     9481 cctgtaatca aggaacgtga aggattgggg acaggggctt ccgcaaaaaa ttgttcgaaa
     9541 gtcacttgtt tttgtagttg atattctagt tctgtttggg aataacccgt cagccaacaa
     9601 atgatttcat ctacttcctc ttttatacga tcttttctct ccgcttttgc tacatacata
     9661 ggataaacgc ttgcaaaact tgtcgtaaaa atacgatttt gcgccataat cagtcacctt
     9721 tcttttt
//
LOCUS       DAKBVG010000099         9701 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000099 DAKBVG010000000
VERSION     DAKBVG010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.099"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(<1..1030)
                     /locus_tag="OUD09_001690"
     CDS             complement(<1..1030)
                     /locus_tag="OUD09_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCT9692960.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1112..1801)
                     /locus_tag="OUD09_001691"
     CDS             complement(1112..1801)
                     /locus_tag="OUD09_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCT9692961.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="OUD09_001692"
     CDS             complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="OUD09_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCT9692962.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2328..2831)
                     /locus_tag="OUD09_001693"
     CDS             complement(2328..2831)
                     /locus_tag="OUD09_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCT9692963.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(3035..3162)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="OUD09_001694"
     CDS             complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="OUD09_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCT9692964.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="OUD09_001695"
     CDS             complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="OUD09_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCT9692965.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4799..5467
                     /gene="sdaAB"
                     /locus_tag="OUD09_001696"
     CDS             4799..5467
                     /gene="sdaAB"
                     /locus_tag="OUD09_001696"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HCT9692966.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5480..6352
                     /gene="sdaAA"
                     /locus_tag="OUD09_001697"
     CDS             5480..6352
                     /gene="sdaAA"
                     /locus_tag="OUD09_001697"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HCT9692967.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6443..7462)
                     /locus_tag="OUD09_001698"
     CDS             complement(6443..7462)
                     /locus_tag="OUD09_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HCT9692968.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7635..8540)
                     /locus_tag="OUD09_001699"
     CDS             complement(7635..8540)
                     /locus_tag="OUD09_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HCT9692969.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(8599..9144)
                     /gene="nusG"
                     /locus_tag="OUD09_001700"
     CDS             complement(8599..9144)
                     /gene="nusG"
                     /locus_tag="OUD09_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCT9692970.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9245..9415)
                     /gene="secE"
                     /locus_tag="OUD09_001701"
     CDS             complement(9245..9415)
                     /gene="secE"
                     /locus_tag="OUD09_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCT9692971.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9439..9591)
                     /gene="rpmG"
                     /locus_tag="OUD09_001702"
     CDS             complement(9439..9591)
                     /gene="rpmG"
                     /locus_tag="OUD09_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCT9692972.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
BASE COUNT         2738 a         2052 c         1775 g         3136 t
ORIGIN      
        1 atacagtatt atttcggttg cttgctgctt cacctagccc ttgtactcgt gtttgattgg
       61 cgacttgacc aaattgattc ccgacgtcta ataaaataat agctaaaagc aaaatggcca
      121 aatggcttcc gccaaccaat aaagcaataa agcttattgt ttgcataaca agtcccatat
      181 acacgatttt tctttcagaa taagaatctg ccagtcgtcc cacgataggt gcagcaactg
      241 ctccagacaa accaaaaatc gctaatatcc ctgcttcgaa tgttccccaa ttgtaggcgg
      301 gactgctgat ataaaaaatc aacgtagacc aaaagatcga aaatgttcca aacatgaaaa
      361 atccactgac tgccgcttct cttaatagac gttgagaagc aactaaatgc ggcaaacttt
      421 taagtgaatc aaggtatgaa acagctgtac tactttgctg gacagaacgc ggcattttcc
      481 agtaaagcag aagtgtaaga attccgactg cacataaagc aaataaatag atcatccgcc
      541 aagaaaaaag actggcaaca ataccagata ccgtccgaga aagcaaaatc cccacgagta
      601 aaccgctaag caatgtaccc atgaccttcc ctcttgcttt tggtcccgct agtaccgcac
      661 cgtaaggaat gatgatttgc ggcacgatcg aaagtaatcc tattaaaaaa gaagcgcatg
      721 caaataacgt aaacgaaggc gcaaaaaaag cggccaacag agagacggca gataacgcta
      781 ccattcgaat gatcaatttt ggacgatcca ccacgtcccc taaaggaact aaaaacagta
      841 accctaacgc atagcctaat tgggtcaaca tcgtcaatat cccgactgca cttgtgctaa
      901 cagataaact agctgctact tttgttccaa tcggctggat gtaatacata ttggcaacaa
      961 caacgccaca agtgattgat aatagtaaaa taagcgaagt tgttaaaggt gttttttcat
     1021 tactcttcat tgatttcccc catttcctat cttagtttac aacttatgga aaagattgga
     1081 aatagaaaaa tacaactatt cgccacactt attatttgtg cttttgtttt ccttgttttt
     1141 tcttgcgatc tttataaatg aagacaacta accctactag cacagtaaca cttgcaacca
     1201 acgtgactgc agttttgtta tatccgtgaa acagagcttc tccaccacac gcatacaaca
     1261 cagacgaagg gatgactcca atcacgatcg acaatatgat ttgctgtaga ggcaatttca
     1321 tcgcatctgc agcaaaattg accatagaag atggaataac cggtatcata tagcctaaca
     1381 ttacacctat ttttggatgt ttcatttgtc ggattgcttg cacccaatga ttcgtttcgg
     1441 ttttcttatc taaaaatttt aagcgatgca taaggaaaat tgcagtgatg ttaccaagag
     1501 cattacccaa aatgttgatg atcgcacctg tcagtggtcc aaagctcaaa ccagcaatga
     1561 ctccaactac tgaagtagga atacctggaa cagcacacat caccgctgtc aatacgatca
     1621 agagtatcga tgcaaaagtc ccatgcgaac gaatatgtgc gagtagctct gccttactcg
     1681 ctctcggatt taaaaataat tggatgtctg tctgatattc taaatacaac cgatagccca
     1741 ctaccagtat cagaatgatt cccaacgaga tcaacatcaa tcggattatt ttctttttca
     1801 tatttcctcc tgaaaaaaat ctatattcat taaataatag tttaacataa aaacaaaaaa
     1861 gcaccgaaat ccaacatagg atctcagtgc ttttaaatag taaaaattat ttaacagtta
     1921 cagatgcgcc aacttcttca agtttagctt tcaattcttc agcttcttct ttagatacgc
     1981 cttctttaac tggtgcagga gcgccatcaa ctacagcttt agcttctttc aagcctaaac
     2041 cagttgcttc acgaactgct ttgataactt taactttttg gtctccagct gcagttaatt
     2101 ctacagtgaa ttctgtttgt tcttcagcag cagcagcgcc accagcagca gctacagcta
     2161 caggagcagc agcagatacg ccaaattctt cttcaatagc tttaactagg tcgttaagtt
     2221 ctaggatagt tgcttctttt aactcagcaa cgatgttttc aatgttcaat gccattataa
     2281 tttcctccgt ttaattgttc ttttttattt aatgctttat gcggcaatta agctgcatct
     2341 ccatcttctt tgtctgccac tgctttgaca gcgtaagcca cgttgcggac tggcgcttgt
     2401 agtacagata gcaacataga tagaagtcct tcgcgatttg gcaattttgc caatgctgtg
     2461 atttcttcca atgaagagac tttaccttcg atgataccgc ctttgatttc taatgctttt
     2521 gcttctttag caaattcgtc catgattttc gctggtgcga ctacatcttc gttactgaat
     2581 gcaacagctg taggtccagt gaatacttcg tccatgcctt caaggccagc ttttttagct
     2641 gcacgtgaaa gaatagaatt tttgataact ttcatttcta cgttagcatc acgaagttgt
     2701 ttacgtaaac gtgttacttc atcaactgtt aatccgcggt agtcaacgac tactactgat
     2761 gcagctgctg taaatttttc gcttacttca tcaacgattg ctgctttttt tgcgataatt
     2821 gcttcactca tttttgagtt tcacctcctg aattttgatg gaagagtttt ttgcactgaa
     2881 aaggcaagtc acttttatag ttactttgct caaccagtat tattcatcac ctattcactt
     2941 cgttcatagt gattctactc gctttgctcg aaaggaaact cctttcgctt tgctcaacca
     3001 gtattgttca tcatctcttc acttcgttca tagtgattca caataaaaaa ctctatgcca
     3061 ccgcagacat agagggacta ttgaatatat tcaataaacg tcctcggtag gaaattaagg
     3121 cttgatgcca cctactgtct tcggtacaag ttaattatta acctcaacta gcttagcatg
     3181 ctaaactagt tgagtcaaga attatttttc agttggatca atcaacattc taatatttaa
     3241 ttagaaagaa gcttggtcaa catggatacc aggtccaaat gttgttgtga tcgttacatt
     3301 tttcatgtat tgacctttag ctgctgatgg tttagctttt agcaatacat catggattgc
     3361 tgcaaagttt tcaactaatt tttcatcgtc aaatgaaact tttccgattg gtacatggat
     3421 atttccagct ttgtccacac ggtaagttac tttaccagct ttcacttcgt ttactgcttt
     3481 tgttacgtcc attgtaactg taccagtttt agggtttggc attaagcctt ttggtcctaa
     3541 tactcgtcct agacgaccta cagtagccat catatcaggt gttgcaacaa ctacatcaaa
     3601 gtcaaaccat ccgttttgga ttttttgaac catgtcgtcg tcacctacga aatctgcacc
     3661 agctgcttca gcttcttttg ctttgtctcc tttagcaaaa actaaaacag tttgtgtttt
     3721 accagttccg tttggtaata caacagcacc acggatttgt tggtctgctt ttttagggtc
     3781 tacgttcaaa cggtatgcaa cttcaactgt tgcatcaaat tttgcaatgt ttgtttcttt
     3841 tgctaaagct actgcttcgg caacaggata aactttactt gaatcaactt tttttaatgc
     3901 atcttgcatt tttttgctct ttttagccat tttggttcct ccttgattgt ggttataacg
     3961 gttgtacctc ccacgtgttc tatatctttc aatatagagc caactgagaa gctgctgtct
     4021 tagggtgtat agattattct acagtgatcc ccatgcttcg tgcagttcct tcaaccatgc
     4081 gcattgctgc ttcaacgtta gctgcgttta ggtcttccat ttttaattca gcgatttctc
     4141 ttacttgatc gctagaaact ttcgcaactt tatttttgtt tggttcgcct gaaccttttt
     4201 cgattttagc tgcttttttc aatagaacag cagctggcgg tgtttttgta acgaatgtga
     4261 atgaacggtc ttcgtataca gaaataacaa ccggaatgat caaacctgct tgatcagctg
     4321 tacgagcgtt gaattctttt gtgaatccca tgatattgat acccgcttga cctagtgcag
     4381 gacctactgg tggagctgga gttgctttac ctgcaggaat ttgcaattta acgatttttt
     4441 ctactttctt tgccacgaga catacctcct taagtccgtg atgtggttaa ttggagatgc
     4501 atatttctcc tcccacgttt tatctgatct gaaaaaatcc agacaaataa tgtgcaaaac
     4561 aatgcacact ggtacagtat agcaaattta tttgtttttt caagattttt ttctgctatt
     4621 tttacatttt gcttgaataa acgctaaatt tattctaaaa aaacaacaat ttttcgttct
     4681 catttcttct tttatgttac acttgcgcta gcatggttca acagatgaaa gcaaacaata
     4741 acacggtttt ttagaccctt gtacaaagat tcctacataa caaaatgagg tggagaaaat
     4801 gaaagattta aggtatagaa gtgtctttga tattattgga cctgtgatga ttggtccaag
     4861 tagctctcac actgccggag ctgctagaat cgggaaaatc atacgtagta tatttggcga
     4921 acagcctgat tctgtagata tttatctata tgaatcattt gcaaaaacct atcgggggca
     4981 cggaacagat attgcattag ttggcggcct gttagggatg gaacccgatg atgaacgttt
     5041 agctgattct ttgaaattgg catatgaagc cggtatggaa gtcttgtttg ttcctaagag
     5101 tgaaaaagcc gaacatccaa actctgtaaa gatgcttcta aaaaaaggaa cacgaaagct
     5161 ttcagtcact ggcatttcga tcggcggtgg caatatccag atttctgaat tgaatggatt
     5221 caaaatttct ttaagcatgg gtactcctac cttgatcgtt gtccaccaag atgttccagg
     5281 tatgattgct aaagtgacta gtatactttc tgagaccaat acgaatatcg gcactatgac
     5341 agtgacacgg gaatcaaaag gtgaaaatgc cattatgata attgaagtag atgatcctca
     5401 agtagaagaa acggtaaaaa gactcaaaca gctgccaaat atcgacagcg taaattattt
     5461 cgattaagga gctaaaaaaa tgtttttatc gattgaagaa ttagtagatc aagcaaaagc
     5521 ttatccaagt gtagcagaat tgatgattgc agttgaaatg gaaatgagtg gccgttcccg
     5581 tagtcacatc atcgagatca tggaaaaaaa cctctcagta atggaacaat caatcaacga
     5641 gggaaaagaa ggtgttacct ccgtcacagg aattaccggt ggtgatgcaa aaaaattaga
     5701 tgattattta aatgaggcag actttttaag tggagaaaca atcttaaaag ctgtacgcaa
     5761 tgctgtagct gttaatgaag tcaatgcaaa aatggggttg atttgtgcta caccgactgc
     5821 cggaagcgcg ggtgttgttc ctggtgtgct gatggctgtc cgagatcggc ttgatttgac
     5881 acatgaacag caattggaat tcttatttac cgctggcgct ttcgggttag tcatcgcaaa
     5941 caacgcttct atcagtggtg cagaaggggg atgtcaagca gaagttggtt ctgcaagtgc
     6001 aatggcagca gcagctttgg tttgtgcaaa aaatgggact cctcatcaag cagcacaagc
     6061 agtagccatt acattgaaaa atatgatggg actgatttgc gatcccgttg caggcttagt
     6121 tgaagtcccg tgtgtcaaac gtaatgcttt aggctcttct caagctttta tctctgctga
     6181 tatggcatta gcgggtatcg aaagtgtcat tccgccggat gaagtcgttg ctgcaatgta
     6241 ccaagtcgga cgtcaaatgc cgtcgatttt caaagaaaca gcagaaggtg gcttagctgt
     6301 tacgccaact gctaaacgac ttgcggaaga gattttaaat aagagggcgt gataaagctc
     6361 cttaatttcg agtattaaga gattagttat cttcataaca aaaaacgaag agaggactaa
     6421 ttgcccttct cttcgttttt tattaatcca tcaatttcgt attttttact tcatcgatcg
     6481 tttttgttcc tgcaagttgc atcgtgatcg acaattcttt gttcaggtgt tcaaaaacag
     6541 atgttactcc ttctgcccca cctagattca acccataaat cactggtcgg ccaatcgcaa
     6601 ctaagtcagc accacttgcc agagctttga agacatgctc tcctcgacgg acaccagagt
     6661 cgaaaatcac tggtacacgt ttgttgacta cttcggcaat ttttggcaat acttcaaagg
     6721 acgcaggacc tccatctaat tgacgtccac catgattgga tacccagata ccatctgccc
     6781 ctgcagagat agcgattact gcatcttctg gcgattgtat tccttttacg ataactggaa
     6841 gatgggtgat ttctttgatt gttttgatgt catctggtgt caagccttgt tttgcagctg
     6901 cataaatttc tgcgattcct tttccttcgc cattaccact tgcactttgt tcgctatacg
     6961 cagctaagtt aggcattggt aatggaaatt ggaattgatt gatgacgtct tcttcacggt
     7021 aaccaccgag cgttgaatct gccgttaaaa tgatagcttt agcccctgct tctactgctt
     7081 tgttcaaaat gaactcattg aatctgtcgt ctttactcat atataattgg aaaaactgcg
     7141 gtgcgcctgg cgctgcatcg gctgcgtctt tgattgtcgt atttgcataa gtgctgatgg
     7201 aaaagattga tccagcagca gctactcctt tagctgtatc agcttcccct ttttcatgtg
     7261 ccaatccttg ggccgctgat ggcgcttgga taatcggtgt atctaattca attccgaaaa
     7321 cagatgtatg caaatcggct gagtcgattc cgcgtaatat ccgtggcatg attttctttg
     7381 tattaaaaga agtcgtattt tctttcatcg tccactcatc ttcagaacca ccgcgaatat
     7441 agccaaaagc tcctgcttcc atgcgctctt ttacacgacc ttctagttag gcaatgttta
     7501 caatatcgat tggatgttct tcattacttg tatgataagt catagtagat ccttctttca
     7561 tcttcttgat tacatataaa tcatactaaa cttacaaaaa gtaagcaaac atttactctc
     7621 agatactttt atcgctattc tttccaaata aatcgactta caagctgatc tacttcttgg
     7681 ttttcatgaa gcatactatg agaggcattt tcacctgtca ctactttttc ctctatttca
     7741 tttccgcgtt gcttcaacaa cgcatagacc gctaatgcac tgtttaacgg tactgttcca
     7801 tcactcaagt ctgtttcatc tagttgacca gcaatcaaga aaaatcgagt ggttatagga
     7861 atattaccga tcatttgctg gtaatcttga tagcggttag aaacgacagc aggccccttt
     7921 gctaactcat ctgtcagaga ctgcgcacta tcatcgacaa aatcattaaa aggcgcgcca
     7981 attgctgtta atgtctgaac ttgtggcaat gacgtatcct gtccatacgt tcctaaatag
     8041 cgtagtgcac taactcctcc catggaatgc cccactatgt tgactttatc tatgccgtag
     8101 ttttctttca gatacaaaag acaagttttg atccattctg cttgattcca ctcgttattt
     8161 ttattatctt caaacaatac ttggaccatc ggattgtctg cttttccaga caaatctcct
     8221 gttgcttgta tttgtccatc tgttcctact gttaaaacca attcttttac tgcttgctga
     8281 tttttttcca aacgccgcaa catcccttta aaggaatttt ctgtcccgct gtagccgtgg
     8341 aaaaaaagtg ttggaatagt ggttttttct atcactttac tggattctgc cgttttttct
     8401 ttatcagaaa cttcagtagt atttatcttc gtcgtctggc aaccacttaa cgcaaacaaa
     8461 ctgataagga ctaaagctgc tgcagttgca cgtttgcata aatttttgat tggttctgtc
     8521 atacacctct tcctttccat atagaaaaaa gaaagacttg aaaggccaat catatgatcg
     8581 acatttcaag tctctttatt aatcgatttt atctacttga tcgaaatcaa gttctgtact
     8641 ggtttcacgt ccaaacatat cgatgttgac tttcaatttt tctttttctc cgtcaacttc
     8701 tgttacttgt ccttctaatc cagcaaatgc gccttcgatg attttcacga cttcgccaac
     8761 ttctacttct agatcatttt gtcgcaggct cataccgata gaacgcaaga tatgattgac
     8821 ttcttctgga agtaatggtg ctggtttgct tccggctccg tgtgaaccaa cgaatcctgt
     8881 cacaccaggt gtgttgcgga caacatacca tgaagcgtct gtcatgatca tttcaacaag
     8941 tacgtatcct gggaatgttt tatgaacgat ttctttttct ttcccatttt tgacttcagt
     9001 ttctttttct tcaggaacaa cgacacggaa aataaagtcg gccattttca tactttgcgc
     9061 acgagattcg atatttgctt ttactttgtt ttcatatcct gaatatgtat gcaagacata
     9121 ccagtttttt tcaaaagatt ccattgaagg ctcctcattt cattgtaaaa taaaaaaacc
     9181 tcagtacacc cgaagttttt ctttcgcttc attatagcac taaaagtacg ttgtggctag
     9241 cgagttattt taaaatccag ccaaataagc tttggatggc tgtatccatt acgtaaaaca
     9301 tcaatccgaa aataatagaa gtttcgatga ctactaacgt atctttacgg agttgttttc
     9361 tgcttggcca agttacttgt ttgatttcat cttttacgct ttttagaaat ttcatgagac
     9421 aacctccctt gctactgttt atttcgtttc tttatgtaat gtatattgat tacaatattt
     9481 acaaaactta ttgatttcca aacgttcgcc gctttttcct tcactgactg attttgtgta
     9541 gttgcgagac ccacaaacgg aacaagcgag tgctgctttt ttctttgcca tttacttctg
     9601 cccccttgct gagaatcaga cttatacttc ataacagtat cacgtaactt tttacttgtc
     9661 aatttgtgaa ataaactatc tattgttttg gataaaatta t
//
LOCUS       DAKBVG010000100         9630 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000100 DAKBVG010000000
VERSION     DAKBVG010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.100"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            185..1183
                     /locus_tag="OUD09_001703"
     CDS             185..1183
                     /locus_tag="OUD09_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCT9692973.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            1295..1804
                     /locus_tag="OUD09_001704"
     CDS             1295..1804
                     /locus_tag="OUD09_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HCT9692974.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(1834..2493)
                     /locus_tag="OUD09_001705"
     CDS             complement(1834..2493)
                     /locus_tag="OUD09_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HCT9692975.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(2495..3259)
                     /locus_tag="OUD09_001706"
     CDS             complement(2495..3259)
                     /locus_tag="OUD09_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HCT9692976.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(3267..3944)
                     /locus_tag="OUD09_001707"
     CDS             complement(3267..3944)
                     /locus_tag="OUD09_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HCT9692977.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(3981..5366)
                     /locus_tag="OUD09_001708"
     CDS             complement(3981..5366)
                     /locus_tag="OUD09_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCT9692978.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(5576..5660)
                     /gene="ffs"
                     /locus_tag="OUD09_001709"
     ncRNA           complement(5576..5660)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="OUD09_001709"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="OUD09_001710"
     CDS             complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="OUD09_001710"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HCT9692979.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            6305..7162
                     /locus_tag="OUD09_001711"
     CDS             6305..7162
                     /locus_tag="OUD09_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCT9692980.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(7243..7578)
                     /locus_tag="OUD09_001712"
     CDS             complement(7243..7578)
                     /locus_tag="OUD09_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9692981.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(7645..9015)
                     /locus_tag="OUD09_001713"
     CDS             complement(7645..9015)
                     /locus_tag="OUD09_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HCT9692982.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(9093..9629)
                     /locus_tag="OUD09_001714"
     CDS             complement(9093..9629)
                     /locus_tag="OUD09_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCT9692983.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
BASE COUNT         2822 a         1985 c         1618 g         3205 t
ORIGIN      
        1 atttgcatac ttcataataa taataacata atacctaacc tctcgtaaaa atactagatt
       61 tgttttcttt tatttttcat cactagatac attctataac ttaccgtttc gatttcgaat
      121 tcaaacattt ttgagaagca ttctcaatta aattctgcta tagtgtgaaa agggaggaat
      181 ttttatggaa atctatgaca ttacaatcat cggtgcaggt cctgtaggga tgtttgccgc
      241 tttttacgca ggaatgcgcc aagctaaaac aaaaatcatt gacagtctac ctcaacttgg
      301 tggtcaatta gcaactttgt atcccgaaaa atatatttac gatattcctg ggtatccagc
      361 aataaaagca agtgaattaa tcgatcaatt agaaaaacaa ttaactactt ttaaccatac
      421 attccattta aaagaagaag ttctctcgct tactcgtgaa gacgaagtta tcgagattac
      481 tacaaataaa ggcatccatt attccaaagc agtcatttta gctttaggaa atggttcatt
      541 ccagccaaga aaattgaatt tagataatgc tgaatcattt gaaaaccacg gattggatta
      601 tttcgtgaat gatttgatga agtatgctgg aaaaaaagtc gctattgctg gaggtggtga
      661 ttctgctatc gattggacgt tgatgttaga gcctattgca agcgaagttt acttgattca
      721 tcgtcgcccg gaatttcgtg cgcacgaaca tagtgtatca cgcttgaaat cttcatctgt
      781 caacttgctt actccctatc tcatcgatgg attatcaggc aataatgggg aactgacaga
      841 tatccggtta aaaaaagtga aaagtgacga gacgattgat ttgatgattg attctctgat
      901 cgtcaattat ggattctctt ctaatttaga acatctttct tcttggggac ttgatagtac
      961 cagaaatgcc attactgtaa aatcagatat gtctacttct attccaggcg tttatgcagc
     1021 tggtgacatc tgtacctatg aaggaaaagt gaaactgatt gctactgggc tcggtgaagc
     1081 acctactgca gtgaacaacg ctttacacta tattaatcca aaggaacgaa ctcagcctgg
     1141 tcacagcaca agcttatacg ataaaaatct tcgtcctagt tgatttttta tatctctttg
     1201 catttcaagt taaacccgaa agcagtttct tgcatttttt tcatgttccg ttaacatgaa
     1261 atagagaaag aaaaaataaa gggggagcaa taatatggtc gtcaaaactg agactttaga
     1321 agaaaaggca agagaactat tgaaggaacg tggcgtaact gtagaagata tcgcacaact
     1381 tgtcatgttt ttacaaaaag actatatttc tgatttgact ttggcggatt gtactgaaag
     1441 tgtcaacagc gtgctaacaa agcgtgaagt acataacgcc attatcactg gaatccaatt
     1501 agatatatta gcagaaaaga aaaaattgat gagtccgttg caacatatta tcgaagatga
     1561 cgaaggcctt tacggtatcg atgagatcct agccttgtca atcgtgaatg tttacggtac
     1621 gatcggcttt acgaattatg gttatatcga caaggtgaaa cctggtattt taaaagaact
     1681 aaacagccac gatggcgttc atgtccatac cttcttagat gatatcgtcg gagcgatcgc
     1741 cgctgctgct gccagtcgat tagcacacaa agaaccagaa aaaagaagtc gccttactca
     1801 ataattcaac aaaaaaagaa agaaaacgtc tatttagacg agttctttct tttttgtttg
     1861 tttcaagcta ttttttccta agaaaagaaa aacagcaaaa aataattcta ataatacaaa
     1921 aaataagata aagctatgca tgaaaaattc ttctggaagt acattgatga ttggatcagt
     1981 aacgggatcg aataaccagt catcgttgtt gaaaaacgtt tcatgaaaaa ggataaaaaa
     2041 gcgatcaaat cctatcatca tgaaaaaacc gaaaacaact ggcagtaaca tgccgatttg
     2101 aaacggacga atcaaccgcc aaagccgatc attcttttgc aaatatttga taaacaaaat
     2161 actcgggatc aaagtgatga aaaacacagc ataatccact aaaaacaaaa ttttcacttc
     2221 ataaaagtga tgggctccgc tagcagaaac tgggaaatct ggcaagctga ggatggtttt
     2281 gaagggatta ttgagataat tcattaggtg atcaaaattt ttcagtaacg tctcttgatc
     2341 taaagaagta taatccaaaa tttgcaaatg tccgatatca aatacgtata gtggtcgaaa
     2401 gttgatcgtc acaccaactg ccagactgat cagtgtcaga aataggctga acaatccagc
     2461 atattccatc cactgccatc tcgaattttt catattagaa gctccattca tccagtgatt
     2521 gtttcacata ggtagctggg ataggcaacc caggtacatc ttcttctttt gtaaaacctg
     2581 ataaaacgag caaagtatct atattattgc gtatcccagc ttggatatct gtttcatagt
     2641 tatctccaac catgattact tcttcttttt gcagccctaa tacttcaacg gctttatcca
     2701 taatgatggc ttctggtttt ccgatgtaga tcggcggcgt ttgggttgct gtctctacaa
     2761 aagagatgac tgaccctgct ccaggaagaa ggccgcgttc agtaggtata ttcttatctg
     2821 ggttcgttcc aatgaaagta gcaccttttt gaatagcaag tgttgcttta actacttttt
     2881 cataggtcag ataattatct aagcctacca ctacatagtc aggagcctct tcttcccatg
     2941 taaatccggc agctaaaatc aagtcaatca atcctgcttc tccgataaca tagacttttt
     3001 tcccttttcc atctgctttc ataaaatcga ttgttgccaa agtagctgtg tagactgtct
     3061 ctggagctac atgaatgtca aactcatctg ctagacgatt agctactgtt tctggtgact
     3121 tagtcgtgtt attggtaaca aacaagaaag gtaattttct tttttgcaat tcttccacaa
     3181 atcgtttacc ggccggtatg ggttgtgtcc cacgataaat cgttccgtct aaatcaatta
     3241 aatatccttt atagtccatt catccactac tcttcccttt ttctgatcgt aaatgctgtt
     3301 tttgtttttt ctacttgttt aggttgttct tgttttttta ctttttcaga ggtttttgct
     3361 gactctgttt ttcgattttt aataaccggt tttttgcgat tagatgcgac tggtcctttt
     3421 ttctcatcga tatacgcacg attatggttt tttttctttt ttcggcgctg agttgtcttt
     3481 tcttttttac cgccgattct ctcgatgaca aaatacgcac aaccaaaatt gcaatattcg
     3541 taaagatagt cttccaagga atcgattcgc tgatctggat gcgcccgtcg attatccgct
     3601 tggaaaaatc cttttaggcg tagttgttca taaccccaat ctcctacgat ataatcatat
     3661 cgggctagta catcactgta acgttctcca agcttttctg cattaaatcc ttcacgataa
     3721 tcggacacaa gccgataatg tctttcacct atcaaaaaat tactgtcatc taacagtgtc
     3781 actagctcgc ctttgacttt ttctgcttta ggctcctcaa cgatttcttc caaagcagct
     3841 gtcagttctt cggtgactgt tgtttcctgt tcagctgatg attttttctt agcaatttct
     3901 gttttatgct caacgttctg ctgttcattt tgttttgcag acatgcgttc accacctatc
     3961 ttttttatta taccttgttt ttatttgatt gggtagtgag ctttcaagta atctcctacc
     4021 acacttcgga taaattcagg gaacaaaaat tcattttttc cagcaatctc aatcgttgga
     4081 aaaaatggaa caaacatcaa atgatctacg gtagtaaaag tataattttt attcagatcg
     4141 attggcttgt tcttccaaaa gacttgatgg ttaatagaat catatgtgat tccattatag
     4201 accatctgcc caaaaatctt accgcgaaat cccattccct tcataggata gttacgtaaa
     4261 aagttacgat ttttttctat ttctaaaact aagcggatca gatcatttcc acccaatgtg
     4321 acatttaaca agtgcatcgg atgcggcaac gcagtgtgaa gctgatcctg attgacccgc
     4381 ccttttggaa tttcagttaa aaacagacca ctgtttaata ttgctgcttc cgtctttcct
     4441 cgttctttca ccgcctcaaa ggctacttgg ataaacgaat gctcattgaa gatatctaga
     4501 ctcagagaaa atggcaaatc tgctacttct ttttctttta ataattcatg cccacgttct
     4561 agataactat ttatttcttg ttcatcttct gtaaaagctg tcattgtttc ggtagggatg
     4621 gctttggctg aatgcttaag gatttttttc tcttcatcga tggttaaatg gacttcaccg
     4681 acatactgcc cgtactttcc agcggctgct atttggacat cattgattac gtgaccttct
     4741 ctgaataaat gatgggtgtg cgatcctaaa ataacatcaa ttgctggtat ttcttttgca
     4801 atttggaaat catcctgaat cccgagatga ctcatcaaaa ctaaaacatc cacttgtgaa
     4861 cgtaaatctt cgactaattc tggcaaaata tcataaggat tccgtatgtc ccagccattc
     4921 ggattataag taagcggaaa tggagcagta aatgcaatta gtccgatttt cgtctggtgt
     4981 ttcgttgtaa tgattttata tggtttcgtc catgccggtg gctggagcgt atttttatcg
     5041 aataagttat ccaggatgat atcaaactca gcatgatcgt ataaatgatt caactcttct
     5101 ttggaatttc ctacgccttc attgtttcct atcgtcgctg catcataacc aacttcattc
     5161 atcagcaaga tattttcctg tccattcgtt gcttctgaca atggatgcca acgatccaca
     5221 aaatcaccta gatctactgc aatcgttgtc gttttttctt tcttttcgtt ttctcgttta
     5281 cgttgatcca aaaaccttct gatccttggc caattttcta aatgagaatg cagatcattc
     5341 gtatgtaaaa tcactatttt ttccatgtac cctctcgcct ctctttatct ttatcatacc
     5401 ataggcagct ttttcttcca ttgaaacaag ctctatttca gagtttttcc tgccttgaag
     5461 caaattgtat aagtacaata ttcgataaat cagtctggct aaaaaatcag gaacattcat
     5521 ttttacagta ctataaaaat aaaaaaaaca gacccgaaag tctgttgtcg tttcattata
     5581 atcagacaca tggcggaacc tgcttagtgc tgcttccttc cagacctgac acgcttcaca
     5641 agcccaccat tgtcctagcc tttcggcaaa gaatagtata ccttattttt cattcgttga
     5701 ccagtctttt tttaatttct tttttcgctg gcggagcttt cggaaaaagt cagtcaaaat
     5761 catcccacat tcgtcttcta aaatgccacc ttctacatat gcttggtggt taaaccgctc
     5821 gtcctccaaa agattcatca aggtaccggc tgttccgcct tttggatcat acgcgccgaa
     5881 ataaacttct gctacacgag acaaaatcat cgcgccgctg cacataggac aaggctctaa
     5941 ggtaacaaaa agtgcagcat cttccaagcg ccagcttccc actttttcac aggcttcctt
     6001 gattgcaagc atttctgcat gtgtagtagc atcttgcgtg gtttctctaa ggttatatcc
     6061 tcttccaatc acttttccat ccaatacaac cactgctcca atcggaactt catgaagcat
     6121 ttctgctttc tttgcttctg ccaatgcaag gcgcatccat ttttcttttt ctactgcatc
     6181 aagatttgta tttaccaatt acccttccct gcctttgttt tttcttgttt tactttagca
     6241 tatgataaaa aggctgtaac taactttttt ttctatggta aaataaatac aagaggtgaa
     6301 caagatgaaa atggataaat tacttgctct ttacccacaa gcacagaaaa aaaactctcc
     6361 agcatcagaa gcggatatcc tttcttttgc aattgatgat gcttttatct ggatcaaaca
     6421 agattcatta tctcaacaag aaaccagcct tttgaaagcg ttattccctg taatcaacga
     6481 tcaaaaaaaa cacccatggt atcactatct ttttaaagat gcttcttgtg ttcaagaaaa
     6541 aagctttcgt ttgatccagc ttcacgtcga atcaaaagga gaattcttaa aaaaagaatg
     6601 gcaagatact attttggaat tatttcgcta ttctgctgat ttcttttttt atacagacac
     6661 agatgcgctc ttagtcgaac aaataaaccg tgattatctt gatgctactg aaatcaacgg
     6721 tatttttcta tctttagatg ctgattttga tacgacaacg tctgtattcg ttggttcttt
     6781 ccattcacct ggcaccgacc tcgtcccttt gtttgctgaa gaaagaaaaa tttttttaac
     6841 agagcaaaac aatctttata ctcacagccg tactttttct atgcaagatg tggctttaca
     6901 ttactataca aaagatactg taaaagatag taagttgatc aaaagttaca tagaactgat
     6961 caaacaaaat gaaatggaag tgatcattca agaattatgg aatgaattag gcaatgtcag
     7021 ctctgctgca aaaacactgt ttcttcatcg taatactcta aaatatcgaa tcgaaaaatt
     7081 tcaagaacag tctggtttca atctaaaaaa agccaacgat ttactgtttt gctatctttt
     7141 attgcttcat gaacataatt aaagatagat taaaggggtt gtgacaataa ttttgtcaca
     7201 accccttatt tcgaatgaat agtgttcaaa agctagtttc tcttacatca ttttttcaac
     7261 aaaatgcatt agccggtctt ttacatcttc ggttgaatct ttgattgaat caccataaac
     7321 agagattttc ttttttcctg atttgatttt tgctaacatt cccatcaaag aatccaagtc
     7381 actatctgaa agaccatcta cgatttctag taatttttca tttccatcaa atttgtcata
     7441 gacgaatttt tttactttcg tccgagtagt catatgatgg atcttttcca ttacttttcc
     7501 cgatacaacg actgctgtcg ctacactggc agcggctgct acgcttaatc cgattgttac
     7561 ttttgttgat gttttcataa gtatcagctc ctttttatct atctccatca tagcatagac
     7621 aaaaaaataa acgaactaat ccactcagtg aaatctcaat cctttaggga aaaagttctt
     7681 tactgttcct tttacttgtt tcccaaatcc gacaggtatc ttatctaata ctagcaaaac
     7741 ccatccttga ttcccatcac gttgaaatgt ttctcctgct gtgtaagatt gccattcctt
     7801 ttgagtgatc ggcaagcaag gtatattctc tatctttttt gtagcaagcg ctaatgcata
     7861 gctaggttca aatcgattct ttttgaaatc tcctaaatgc aaacctgtac gtacgacttt
     7921 taaaccatcg agagaagggg ctaactcagg aacttcccaa agatggtcgt tgaacactaa
     7981 aagtcttcca gtagcttcat aatgaaaatc atttgaaaac tccgtccaga gtttttcctg
     8041 ctcttttgtc atttgcactt tacttttttt tcgtgtcttc ttttctttat gcatctgatt
     8101 ttgtccatga aaagtcagct ttgcaacaaa atgtccttca ccctgatctt tatgaggcca
     8161 tatacgtatt gttttttcca aaccagctac gcttccccat tcagaacgtc ctgaactaac
     8221 ggattgagtc aaaggaatct cctcaatcgt tactggataa ttttctacaa gccagctaat
     8281 gatctcttca ttttcttctg gtgcaaacgt acaagtcgaa taaatcagtt gtcctttatt
     8341 ttttagcatt tttattgctg aagacaaaat ctcttgctga cgtttttgac agtatagcgg
     8401 agattcttct gtccactctt ttattgcatt tggatctttt cggaacattc cttctcctga
     8461 acaaggagca tcaaccacga tccgatcaaa aaagcccgaa aaatgaggga caagttctgc
     8521 gggtgcatga tttgtcacta ttgcgttcga aactccccat cgttctatat tttctgataa
     8581 aatcttcgca cgtttcggaa agatctcatt cgtcactaat aaccctttcc ccttcatttg
     8641 agcagctaat tgggttgatt taccaccggg agccgcacac aaatccaaaa ctttttcacc
     8701 gggttttgct gcagcagctg ttccaacgat cattgcactt ggttcttggc tatattcata
     8761 cccagcttga tggaggaaag actttccatt gacagtgcca agaaatccct cattgctgta
     8821 gggagcgggt tgtaattctt cgctatggta agtttggacc atgtccaatc ctgccggttt
     8881 tagtggattc caccgaaatc cttttttcac gcttccttgt tcaagcgcag aaaaaaaatc
     8941 ggatgcttct tcgcctaaaa gaagacgata ttttttgata aattgctgtg gtaacgttgt
     9001 cgcttcctcc ttcattccaa tgtttcgtta gacattttac aatctttttt acgaaaaata
     9061 aaggtggctg atctactttt tcatgttgct gcctaaggtg actgttcctt taagatattt
     9121 tccaagtatt catccgtcac ctcgttttta tcccaaacag cgttcatcat ttcaagaaaa
     9181 tcttgagaag aatcatctga gcgcaaactt cccaatgcat taagccatac caattcaagc
     9241 gacttttctt ctttgatctt cgtgtgacct ttgacatatg agaaaaatgg ccgcttaaat
     9301 agcgaatcca tgtctaaatc agattttgca atgatttcac gaatactttc atggatagaa
     9361 aaaaaatctt tcaataagta gacaatgctt aaaacatcat cggaactgca ttcagctaag
     9421 tagttttcaa aataaacagt gatttcttct aatatcttct cttttaaccc gctgattgat
     9481 ttgaaatgtg tataaatcgg gtatgtagaa caaccgcaga actctgctaa ctctcttgca
     9541 gtaattgact tcattccata ttcttccacg tataccatac ttttgaacag caaatattct
     9601 ttactataat ttgttactct ccccatcatt
//
LOCUS       DAKBVG010000101         9473 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000101 DAKBVG010000000
VERSION     DAKBVG010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.101"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            99..746
                     /locus_tag="OUD09_001715"
     CDS             99..746
                     /locus_tag="OUD09_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HCT9692984.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    875..1091
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1139..3784
                     /locus_tag="OUD09_001716"
     CDS             1139..3784
                     /locus_tag="OUD09_001716"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCT9692985.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="OUD09_001717"
     CDS             4094..5407
                     /locus_tag="OUD09_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCT9692986.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="OUD09_001718"
     CDS             5397..6050
                     /locus_tag="OUD09_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCT9692987.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6797
                     /gene="radC"
                     /locus_tag="OUD09_001719"
     CDS             6105..6797
                     /gene="radC"
                     /locus_tag="OUD09_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCT9692988.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            6947..7147
                     /locus_tag="OUD09_001720"
     CDS             6947..7147
                     /locus_tag="OUD09_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCT9692989.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(7401..9080)
                     /locus_tag="OUD09_001721"
     CDS             complement(7401..9080)
                     /locus_tag="OUD09_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HCT9692990.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(9082..9294)
                     /locus_tag="OUD09_001722"
     CDS             complement(9082..9294)
                     /locus_tag="OUD09_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HCT9692991.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
BASE COUNT         3006 a         1694 c         2029 g         2744 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccctatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttctggtaat gttattccat
     6661 cgcagcagga tatgaatttt accaaaagga tacaaaagtg tggagaaatg atggggatca
     6721 ctgtactgga ccacttgatc atcggacgca aacgatattt tagtttgaga gaagaaggaa
     6781 tgatggaaga gaagtaagtt gctttcttgc aaaaaaaatg aagcaagtgt acaattatat
     6841 tgtaattttg tttgggaata gaagttgaaa catctgttgc acctctatcg tacacgagca
     6901 atgttacaat ttaaattagt gcttttggca cgaggaggat attattatgg aaaacggaac
     6961 agtaaaatgg ttcaacgctg aaaaaggatt tggatttatc tcacgtgaag atggaagcga
     7021 cgtattcgtt cacttctcag ctatccaagg tgacggattc aaaacattag aagaaggtca
     7081 agcagtgact ttcgacgttg aagaatcaga ccgcggccca caagctgcaa acgttgtaaa
     7141 agcttaattt tatctgtaaa tcaaattgat ggtagacatc aatttttacg agagaacaaa
     7201 gcgagtaaca aaaatacctg ccacaagaga caatttctct tgtggcaggt atttttttgt
     7261 atgaaaaagt aaataaattt aagaggctat gaaagagcca ccttgtatca aggaataaga
     7321 acaaacaaaa atggcttctc atgtttttca tttgttttct cttatattca ggacaaggtg
     7381 ctgatttcat aacctcttat ttattcttca tctggtgtca ttaccattgg caaaatcatt
     7441 ggatgtcttt ctgtacgttc gaataagaaa ggctgaagcg cgccagtcat cgcttctttc
     7501 aatttatatt ctgaacagtc aggtccattc aaacattctc ttaaagcgtg gaataagatt
     7561 cgctgagctt cattgatcat ttctcctgat tctcgcatgt agacaaatcc acgagagaga
     7621 atatctggac cagccaagat ttcttttttc ttgatatcca ctgtcgcgac agctaataca
     7681 agaccttctt ctgaaagcag gtgacggtca cgtaaaacga cattaccaat atcaccgatc
     7741 ccgctaccat cgatatacac atctccagcc acaaaatgcc cagcaggacg cgcagaatcg
     7801 ctagttaacg ctaaaacatc gccattttcc atgatgaagc agttttctgc aggaacacct
     7861 acatcttgtg ctagagaagc atgtactttc aacatgcgga attctccgtg gacaggaata
     7921 aaatattttg gcttcatcaa acgaagcatt aatttttgtt cttcttgtcc accgtgaccg
     7981 gaagtatgga tgttattgat ttttccgtga atcacttctg caccagcttc agacagcaaa
     8041 ttgatcaaat gattgacact tgttgtatta ccaggaatag gtgaacttga aaagatcact
     8101 gtatcgtctg gctggatttg gatttgacgg tgagtaccat tcgcgatgcg gctcaacgcg
     8161 gccatcgctt ctccttgaga acctgtacat aggatcatcg tttcattggc aggcaagcga
     8221 ttgatttcgt gtgcatctac aaatgtgccg tctggaactt tgatatatcc taaacgttga
     8281 ccgttgacga tggcattttc catgcttcgt ccgaaaacag cgattttacg accagttgcc
     8341 acagcggcat ctgctgcttg ctgcaaacgg aagatgttag aagcaaagct ggcaaaaatg
     8401 atacgtcctt cgatttttga aaagatcttc aaaatagaat gcccaatcgt tttttctgat
     8461 ttagtaaatg ttggaatctc agcatttgta ctatcagata aaaggcacaa gactccttct
     8521 tccccgatac gagccatttt atgcaagttt gctggttcgc caacaggagt gaaatcgaat
     8581 ttgaagtctc cagtcgctac gatatttcca gaaggtgttt tcaccacgat ccctaatgca
     8641 tctgggatac tatgggtcgt tctgaaaaag ctgacagaag tctttctaaa acggatgatc
     8701 gtatcttcat tgatctcatg gagctctgca tctcgaagca agccatgctc atctaatttg
     8761 tttgtgatca acgctaatgc gagaggacca gcatagatag gaatatttgc ttgtttcagc
     8821 aagtagggta ttccgccgat gtggtcttcg tgcccgtgag taatcaccaa ggcttttact
     8881 ttgtgtaagt tttgtatgat gtagctgtag tcgggaatga catagtcaat tccaagtaaa
     8941 tcgtcttccg ggaatttgat cccggcatca atcaaaatga tttcatcttg gaattgtacc
     9001 ccatacgtat ttttcccgat ttcacctaat ccaccaatag caaagacgcc tgtctcgtta
     9061 tttttgatgg aaagtttcat attaaaactc cactaatgag aaatcagcat tttctttttc
     9121 gtactctaga tgacttcctt ctagaagttg aacgaattca atattgtatg gggtattttc
     9181 ttccacacgt tggcgagcgt ctacatcgct ttttgcctca atgtaaagag attttgtttc
     9241 ttcccgttta gggttgcgta cttttgtttc ttgataataa actttataga tcattgtttt
     9301 gtctccttta tctgcgtaaa gcagtgattt agctagttga tttttttaga caacacaaaa
     9361 cgaggggaca agccacctgg tttttcatat gctttgacta gaatactgga ttcgagtcaa
     9421 agagacaagc ctcgtttttg cttatctgtt cggatatttt ttcaaccgta cct
//
LOCUS       DAKBVG010000102         9441 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000102 DAKBVG010000000
VERSION     DAKBVG010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9441
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.102"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            79..1383
                     /locus_tag="OUD09_001723"
     CDS             79..1383
                     /locus_tag="OUD09_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCT9692992.1"
                     /translation="MLNDINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLS
                     QNSLFLKTCLLYLEEDEPDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPR
                     FTEMERRLLLLNVSYRLGGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILLI
                     GFAISFLRQQVCAVTIDSKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLF
                     NLCNYGFHSYQLIAEDFQQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIH
                     LMRSAWDNYQLFMPEKFYLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVI
                     ELSGILRLTKEHLTIYIVTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQ
                     QSSNRVLCERTLYTPDAVQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1475..1840)
                     /locus_tag="OUD09_001724"
     CDS             complement(1475..1840)
                     /locus_tag="OUD09_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HCT9692993.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1837..2202)
                     /locus_tag="OUD09_001725"
     CDS             complement(1837..2202)
                     /locus_tag="OUD09_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HCT9692994.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2394..2642)
                     /locus_tag="OUD09_001726"
     CDS             complement(2394..2642)
                     /locus_tag="OUD09_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9692995.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2641..2769
                     /locus_tag="OUD09_001727"
     CDS             2641..2769
                     /locus_tag="OUD09_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HCT9692996.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2826..4520
                     /locus_tag="OUD09_001728"
     CDS             2826..4520
                     /locus_tag="OUD09_001728"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HCT9692997.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKMTNTLTQ
                     EEEHVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4524..4643
                     /locus_tag="OUD09_001729"
                     /pseudo
     CDS             4524..4643
                     /locus_tag="OUD09_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            4620..4793
                     /locus_tag="OUD09_001730"
     CDS             4620..4793
                     /locus_tag="OUD09_001730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9692998.1"
                     /translation="MQAFEHRLPRGCEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKVV"
     gene            5171..6304
                     /locus_tag="OUD09_001731"
     CDS             5171..6304
                     /locus_tag="OUD09_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HCT9692999.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQNGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYE
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6335..6655
                     /locus_tag="OUD09_001732"
     CDS             6335..6655
                     /locus_tag="OUD09_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HCT9693000.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6684..7148)
                     /locus_tag="OUD09_001733"
     CDS             complement(6684..7148)
                     /locus_tag="OUD09_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCT9693001.1"
                     /translation="MVTYNWIKLREHPELVKQSAKWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7277..8526)
                     /locus_tag="OUD09_001734"
                     /pseudo
     CDS             complement(7277..8526)
                     /locus_tag="OUD09_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
     gene            complement(8555..8872)
                     /locus_tag="OUD09_001735"
     CDS             complement(8555..8872)
                     /locus_tag="OUD09_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCT9693002.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(8898..9218)
                     /locus_tag="OUD09_001736"
     CDS             complement(8898..9218)
                     /locus_tag="OUD09_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCT9693003.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDIINMADYGMMNGEKVLDQAEK
                     LINK"
BASE COUNT         3045 a         1860 c         1684 g         2852 t
ORIGIN      
        1 ttatgtgggc aattctatgt aaataaagaa gtcaatctag aaaagctctc caatctcctg
       61 catgtttgca aaaccacttt gctcaatgat atcaataaca taaaaaaaga atttgaagaa
      121 cagatcgctt atacacatcg ggaaaaggac cgttacactt tatatttttc agaacatata
      181 cctcgatgca agatcatgca gcaattgtct caaaattcgt tgtttttaaa aacttgtctg
      241 ctttatttgg aagaagatga acctgattat cttcaattaa ctgaatgtga attcatctct
      301 gtcagcaaag cttattcatt aaaaaaacaa gtattagctt attttaatga ctgcgggatc
      361 gaaatcgacc gctactcccc tcgattcact gaaatggaaa gacgtctgct gcttcttaat
      421 gtgtcgtatc gtttaggcgg ttttaattct tgggagctac ctgaatcgtt ttttgaacgt
      481 gccgatcgtt tcattgaatc cgtaacggaa aatagtggca gattttatga taaagaaaat
      541 aaagaaatct tattgattgg ttttgctatc agtttcctac gccagcaagt gtgtgctgtg
      601 acaatagatt caaaatttat cgaagaaatc aaaaaacgtc ctatttacaa ttatgtcgaa
      661 tcagcatggg aaaacactga tttccaaacc tattataaaa aagaagaatt cgcctttatt
      721 ttgacactat tcaatctttg taattatggt ttccattctt atcagttgat tgcggaagac
      781 tttcaacagc tacaccaagt gtttattgac aatactcccg aaataaaaga actagttgct
      841 acctttgaat ctcacttcaa tcaagaatta ttcggaaacc aaccattcga acgtgcgctg
      901 attcatctga tgcgttcggc ttgggataac tatcaattat tcatgccaga aaaattttat
      961 cttctaaatg aggaacagac aaatttatat aaagaagtcc agacaatttt ttccagctgg
     1021 tccagccagt taccttatga tctacggttg aatcctaatt gcatgcgggc ttttgtgatc
     1081 gaattgagcg gaattctccg tctgacaaaa gaacatctga ctatctatat cgtcacaaat
     1141 tcggatgttc attatctgat ttatcgggaa gccttggaag ctgtaactac tttcgatttt
     1201 caagttgcac caacaattta ttcaagtata tctgatataa agaagtatgc gcagcagtcc
     1261 tcaaaccggg tattatgtga acgtacatta tatacaccag atgcggtcca atatgaaaat
     1321 attattccta tttcgatcaa tacgatcgat cgagctatca tttcagctgt gcaaaacaaa
     1381 taagggactg tgacaaaaat catgtcacag cctcttattt gttgaagatg cccatttctg
     1441 taacaatctc ttgatacaca tcaggctcac aacctcactt attttggaca tctacgggtc
     1501 cgatctcttt atttaaaaaa taagaaatga tggttcggat aactaccaac gccgctagtt
     1561 ggaaaatatc ctgcaaggta ggtttgataa tcgattcgat gatcccagct gaaacaagaa
     1621 tttccaaact tagaagtata tagcctccca acatcttttt aatagcattg ttctcgctga
     1681 ctcggtcgct ttgcactttt gtatggacaa ataaatattt caaaaaatcc ttgattgata
     1741 gacaaacacc ccaaaccaat aaacagatag caaagatatc aagtcccagt acaagttttt
     1801 caaaatatgg aagaagaaac tgcatcaact ctgtcgtcat cctacctctc ctcttctttg
     1861 tttttctttg tcttctattt cctgattcaa gaaataagaa atgactgttc gaatgataat
     1921 aataagtgca agcttcaaaa tatctgtcca agtcggcttg ataactgact cgatgatatc
     1981 agcaacgact agtacttcca agcttaacaa aatataactt gctagcagtt ttcttgtatc
     2041 tgcattacga gtattacgtt ctgcatagtt tttcttgaca tgagaccaat caaaaagttt
     2101 gaatagaaca agaacgatcc cgacaattaa gataccgata gaacaaatat ttaaaatagc
     2161 aatgatccat tctagaaatt caattaaatt tgagcgaaac atatacgcta cctactttct
     2221 tttaatttgt tcctattctt ttattgtaaa agaaagttac cttgttcgtc tatttaaaag
     2281 ttgttctaat gataacggtt cgcttaaatt tttctttttc actctcccct ttttctccct
     2341 ccatccataa atcgtggaag tatccaaaac aacgtagcaa gttaaaataa ggcttagtcc
     2401 gtcatcagct tttcaaagaa gcactgtctc ggtgcttttt ttgtacccaa atttctaaaa
     2461 atagaaccat acacctcctc aaatcttttc ttcttaccta ccctattttt atttacagga
     2521 aaatcagaca cttcttcttt tttgtcaaaa tttccttttt cttctcgtcc tatcatttgg
     2581 cattctaata aaaacgaact atccttggat atagaaagag aaaacaagga ggcgtttttc
     2641 atgagtaaaa aattaatcaa agtcggcatc ggtttaggtt tgcttgccct tggcgctgca
     2701 tatctaggaa aaaagacagg actattcgaa gatgacagtc atttatatga tgaattcgaa
     2761 tcaatctagc atcctattta gttgaatcac tacggacata cttggaagac attggaggaa
     2821 tcacaatgag aaaaagtaaa gcaataatga ttggtgccgg tattgccaat atggcagcgg
     2881 ctgtttattt gattcaagaa ggcaaatgga aaggggatca gatcaccttt tattcattgg
     2941 atgatcacgg ttcaaatgat ggtgcaccga ccgacactgt agtggatgaa tattggaata
     3001 aaaaccatcc attagaaaac caaaaaggct atattgctcg aggcggtcgc atgcttaatt
     3061 accgtaccta tgtagatttg atggatctgt taagtcgaat accttctgtt actgagccag
     3121 ggatgactgc tgaagaagat acgagagatt ttgatgcaaa acaccgaacc tttgacaaag
     3181 cacgtttgat ggaaggtggc aaaggaatca ttgatggcgg aaaactggga ttcaataata
     3241 aagaccgtat tcttctgact aaactgattg ctatgcctga ttcagaagaa gaaaaactag
     3301 acaatatcac tatcgaagaa tatttcaaag atgatccaca cttttttgaa acaaacttct
     3361 ggtttatgtg ggaaacaaca tttgctttca gaacacgtag ttctgctcaa gaattgcgtc
     3421 gctatatgca tcaaatgatt tatgaattca ctcaaatcga acatctagtg ggcgtcaatc
     3481 gaacacgtta caatcaatac gaaagtatta tgcttccttt gatcgagtac ttgaaagaac
     3541 aaggatgcaa aatcattttg aaccgccgtg tcactgactg ggaattcaaa gaaacaccga
     3601 tgcaagatga aatcactgtg actggtttga aaatgaccaa tacactgact caagaagaag
     3661 aacacgttgt ggtcgatgaa gatacagcag ttattttcac taatgggtcc attacagatt
     3721 cagctacttt aggagattac gaaacaccag ctgtagaaaa tatggattat ggtgcagctt
     3781 ctagtctctg gaaaaaagcc agtgaacgtt tctacaactt gggaaatcct gacaaattct
     3841 ttgctgatcg agatgcaagc gaatgggtca gctttaccct gacgacgaaa aaccatttgt
     3901 tgttgaatga aatcacccgt atcacgactc aagaaccagg aaatgccttg aattcattta
     3961 tttcaaccac accgatcacg ccgttaggcc aaaaagatgt caatatgtct atcgtcgtgc
     4021 atcatcaacc gcattttacg tctcagaaac caaatgaaac agttatttgg ggttatttcc
     4081 tttatccaag acgacgtggc gaatttgtgg ataaacagta tattaaaatg aatggaaaag
     4141 aaatgctaga agaactgatt ggacaacttt caaaagtaga tcctggacca gtgaatattc
     4201 gtgaaaaaga aacagaaatt ttcgacagcg taataaataa tatccctgtc tatatgcctt
     4261 atgcttccgc gttgttcaat aatcgtgcca aaagcgatcg accaaaagtc attcctaaac
     4321 attcgaccaa tctggccttt acaggagaat ttgtcgagca gccttatcag atgatcttta
     4381 ctgaacaaag tgctgtacgt tctggagaaa tcgctgcttt ccattttgcg ggtatcccta
     4441 tgtcacgtct tgtcaaaaca ccgcgttacg acaaagatat tcctactcta gctcgtgccg
     4501 caaaaaaaat gtttgaataa tcaagaggtt gtgaaaaagc tgccctgcat caagtaataa
     4561 gaacaaccag gctaaaacag cttcttatgt ttttgatttg gttggactta ttaccgcaga
     4621 tgcaagcttt tgaacaccgt ttaccaagag gttgtgaaaa agctgccctg catcaagtaa
     4681 taagagcagc caagctaaaa cagcttctta tgtttttgac ttggttggac ttattaccgc
     4741 agatgcaagc ttttgaacac cgtttaccaa gaggttatga aaaagtcgtt tagtttcgag
     4801 tatgaagaaa aattttgtga aaatggttcc tcacgttttt gcataaattt gacttaatac
     4861 cgagaaactg atcccttctg tcataacctc tttttattta tgtctgtttc tttttgaaaa
     4921 caaccatttt ttcaacgttt actctcttgc caatccaaca tcatctaaca gatacaacta
     4981 tttcaatgat taccattaca ctaaaaaatc ggaaactcac ctctccgaac acaaatacat
     5041 aagactatag aatgtttcat ttaagaaaaa aagcgtttca ttatcaaaaa ggtaagatct
     5101 cctttaaaat gaaattatct gtagtactaa taatgcagac aattttatga atctgaaagg
     5161 agcatctcca atgaaagcag tcacttggca aggaaaacag aaaatggaaa tcagaaacgt
     5221 agatgatcca aaaatattag aaccaacaga tgcaatcatc cgtatcacag caacagccat
     5281 ttgcggctcc gatctgcatc tttaccatca tggtgaagca gttttggaaa aagggtatgt
     5341 tgtaggacac gaaccaatgg gaatcgttga agaagtggga tctcaagttc aaacattgaa
     5401 aaaagggcaa cgtgtggtta tcccttttaa tattagttgt ggtcattgtc atttctgcct
     5461 gaatcacatg gaaagccaat gtgatgaatc aaatccagat cagttatatg gcgggctttt
     5521 cggatttggc aagttgaacg gtaatcattg gggtggtcaa gcggaatatc taagagttcc
     5581 ttatgcagac tctacttcat ttgttgtacc agataatgat cttcctgatg aaaaagtttt
     5641 atttctatca gatattttgc ccacagctta ttggagcgtt gaaaacgcag gagtaaaaaa
     5701 aggcgataca gttgtgattc taggttctgg tccagtcggt ctattcgctc aaaaattcgc
     5761 tgttatggct ggagcagagc gcgtaatcgc agtagatcca gtacagcacc gtctagataa
     5821 agcggctaat tacaatgggg ttgaaactta tttcttagaa gacacggcac aagctggaac
     5881 agaattgtat gaactggcta aaggtggcgc agatgtcatt atcgattgtg taggaatgga
     5941 cggattagag cctgtcaaag aaaaagcgaa aaatttagta agcttacaaa acggcacaat
     6001 ctctccatta cagatggcat cgcaagctgt caaaaaattt ggaacagtcc aagtaactgg
     6061 tgtttatatg actccagctt cttcttaccc gctgcaagaa ttttttatgc gggatatcgc
     6121 tgtcaagcat ggacaggcac caattatcca tctaatgcca aaaatttacg aaatgattgc
     6181 agaaggacta ttcgatccta cacaaatcat tactcattcg ctgccactat cagaagcagc
     6241 acaagcatat gagatctttg acaaaaaaga agataaaaat ataaaagttg ttttgaaacc
     6301 ttgaattcag taaaaaatac aggtgatttt ctttatgccc aaaatcaaac gaaataccca
     6361 tgagatcgaa ataggaaaag aagaagatat cgaaatacga ggacgtcgct tccgcttcta
     6421 tttccaaaat cgttacacat tgatctcact ggctgtcgat ttattaaccg ggattttcta
     6481 tatttttgga agccttgctt ccttaactaa aatccccgat cgatatggca cctattttta
     6541 tttggctggt gctattttct tgacgattcg tcctattctc agaatcgtaa ggaacatttt
     6601 tatttacgac gaaaaaaaag caatgaagag aacaagcaaa aaaaaagagg catgagattt
     6661 gaaaaaatct catgcctctt ttttcaccct atcgtatcac tttgatacag acgagcggtc
     6721 gtcccacttt cttccttgac cattgtcaaa aatgtccact cgtatcgttc ataaaacgtg
     6781 gtatgatctg taattaagta aagtttttcg tacccaagtt gatgcatttc ttttctgatt
     6841 tccgctaata tccgcttggc tatcccctgc ttacgctttt ccggttcaac aaacagggca
     6901 caaagatttg gtgtcaaatc agggcggtta tggaagtcgt tttcaataac gcccgctcct
     6961 ccgacgatgg tttctttttc atctaagacc acataccatt gaggaatact ttccttatta
     7021 gcgattccag tacgaatact ttcctcatac acaacagcag gaactcccca ttttgatgaa
     7081 aaccacttcg cactttgttt tactaattct gggtgttctc ttaatttgat ccagttatat
     7141 gtaaccaatg tttaaacctc cgactaaaat aaaacgttta caaaaattat acaagtcttt
     7201 atgaaaatta gcaatgacaa caatagtgat gtaaaaaaaa cggaaaacaa tcacttgttc
     7261 tccgtttttt gattaatcaa gcatctaaag cttcttttct tctttcttgg atcgtagcta
     7321 ccttgacgaa cggtagatag atcaaaatag aaagaaccaa attgacagcg gctagtacac
     7381 cacctgcaat actttttgtt gccaatacac cgccaataat tggtggagtt acccatggag
     7441 gcataacggt agctgctggc actaaacctg tagaagtcgc aaagtaagca acactaacta
     7501 agaccattgg tgtcaaaata aatggaatga acatgatcgg gttcaacacg ataggtaacc
     7561 caaacatcgt tggttcatta atattgaaga cacctggcgc aatcgacaag tttgttacaa
     7621 ccatatatgg tttgttttta cgtcctacta aataaatagc taacagtaaa cctaaagttg
     7681 cacctgttcc tcctaagttg acaaagctgt caaagaaagg tttgttcacg ataaacggtg
     7741 cgctatgtcc agctgcgatt gcattaacgt ttgcttcaat ggctggcgca ttgattgttt
     7801 gcatcaaagg atcgaccata ttcgctccgt gtaaaccaaa gaaccataag aatggtgtga
     7861 taaatgcaag cagtaaggca gatggataag agtttgcaag tcccataaat ggttcttgga
     7921 ccactgtata gaaagaacct acgatatcat caacaccgaa tgcagcaaaa attgcagcaa
     7981 ctaaagcaaa cgcagagata gtgatcattg ctggtagtaa tgaagcaaat gcttttgcaa
     8041 ctgccggtgg cacaccatca ggcattttga tgatcaattt ttcatttccg cttaaacgta
     8101 caaagatttc tgttgaaata atcccaacaa taatagcaat aaacaaaccg tttgagctca
     8161 tacctaatgc tccgcctaaa gcgaagaaag agcctaaaga aatcacacct gcggaaacac
     8221 cgtcttttcg gtagccgtta gctaaatgat aagcaagaaa gcaatcaaga cagaaagaat
     8281 ggcaaatgta ccgttccaag cagcagcccc gaatcctttc catgattcac caccaaaaat
     8341 tgagttcatc aattcttgga acccgggaat cggtaagtta ttgatcatta ctgctaatgc
     8401 tcctaagatc attaacggca tactcaccat aaatgagtcc cgaatcgcaa ctaaatgccg
     8461 ttgtgcacca atttttgatg ctacaggtat aaaatgtttc tccataaatg caacaaattt
     8521 atccataaat cttccctctt ttctttactg tagattacgc gttcaatcga cgatacagat
     8581 caatgatctc ttttgctaaa tctgtaaaag ctatggatgt catcagatga tcttgactgt
     8641 ggactgtcaa aagcgtgact tcaacgtgat ttccttgtgc ttcttgtgtc agcatctctg
     8701 tttgagagtg atgtgcttgg actaaagatt cctctgcatc tttgattttt tcatctgcta
     8761 aagtgaaatc accttttttc gcagcagcaa tcgcttccat tgcatcgctt tttgcatttc
     8821 cgccaaacat gatcagaccc atgatggctt ctagattttt ttgatcttcc atttattgta
     8881 tcctccgata atttttatta tttgtttatt agtttttctg cttggtcgag tactttttca
     8941 ccattcatca tgccatagtc agccatattg attatatcta aaggaatgcc tttaggtgct
     9001 agcttttcag caaattgatt ttgcataaag cgtacttgcg gtcctaaaag taaaacgtct
     9061 acatttttag attctagttg attatccgca tctgaagcag atacagcaaa gatgtctgct
     9121 tccattcctt ttgcttctgc tgccttttgc atttttgtga ctaggagact tgtactcatt
     9181 ccagctgaac atacaagcat gattgttttc ttttccattt taaacacgtt cctttctata
     9241 cttacacttg tatataagca agtagcgtgc caactttgaa agcgctttaa aaagttgata
     9301 tcatcacatt taaggtctat accaagttgc atacacacta aaaaagaaag tgcacactaa
     9361 acacacaaat taatttttat tacctttttt cttttaacgt gcacaaaaaa agttgtgaca
     9421 caaaacttgt cacaactttt t
//
LOCUS       DAKBVG010000103         9392 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000103 DAKBVG010000000
VERSION     DAKBVG010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.103"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            178..405
                     /locus_tag="OUD09_001737"
     CDS             178..405
                     /locus_tag="OUD09_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCT9693004.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            421..1587
                     /locus_tag="OUD09_001738"
     CDS             421..1587
                     /locus_tag="OUD09_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCT9693005.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            1822..2865
                     /gene="hrcA"
                     /locus_tag="OUD09_001739"
     CDS             1822..2865
                     /gene="hrcA"
                     /locus_tag="OUD09_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HCT9693006.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            2959..3522
                     /gene="grpE"
                     /locus_tag="OUD09_001740"
     CDS             2959..3522
                     /gene="grpE"
                     /locus_tag="OUD09_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCT9693007.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDRYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            3573..5402
                     /gene="dnaK"
                     /locus_tag="OUD09_001741"
     CDS             3573..5402
                     /gene="dnaK"
                     /locus_tag="OUD09_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCT9693008.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            5553..6719
                     /gene="dnaJ"
                     /locus_tag="OUD09_001742"
     CDS             5553..6719
                     /gene="dnaJ"
                     /locus_tag="OUD09_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCT9693009.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(7087..7824)
                     /locus_tag="OUD09_001743"
     CDS             complement(7087..7824)
                     /locus_tag="OUD09_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCT9693010.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(7826..9328)
                     /locus_tag="OUD09_001744"
     CDS             complement(7826..9328)
                     /locus_tag="OUD09_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HCT9693011.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIEKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
BASE COUNT         3070 a         1697 c         2013 g         2612 t
ORIGIN      
        1 tttttttatg aataaaataa ggacaaaatt gtttaggaag aatcatgagt gtaaaaaaat
       61 catccaagaa gtcgattctt ggatttttta gtggatttgt tcgccaaata cgtttagtat
      121 gcgtatatga gaggttgtaa gcaaagcgtt gtggtctgag cacttaagga ggaatctgtg
      181 aaaatagctt ttcatatttt tgcagatttt gacttaatgc cgaaggtcag ctttgcgatt
      241 accgtttatc gagaggctgt aaaagcaccg tgtagctccg atgacaataa gcaagcattt
      301 ttagagctag gtttccttga ttctaaatat gcggcttatt tcaatgagtt ggtgcttgaa
      361 cgcgatttta agaaggtcga aaacaggcac gacttttcga cgtaaggaga aagaatttca
      421 atgatagaaa caataaatcc cgcagaaaac aaggtttctg cgtacattca tatcccattt
      481 tgcgagcata tctgctatta ctgtgacttt aataaagttt ttttagaagg gcagccagta
      541 gatgagtatg tggaaatgct gctgaatgaa atgagattaa tgctggaaag tcatcctaca
      601 gcgaatttgg aaacacttta tgtaggaggc ggaacaccaa cttctttatc tgctaaacaa
      661 cttgatcgac tattatcagg tgctagagag atcctgcctt ttcaaaatgg taatgagttt
      721 actgtagaag cgaatcctgg tgatttgacc cgtgaaaaac ttcaagtgat gaaaaactat
      781 ggcgtcaatc gtttatccat gggagtgcag acatttgaca atcgcttatt gaaaaaaatt
      841 ggacgtaaac atactgcaga agatgtgtat caaacaatgc aatttttaga agcagaaaat
      901 tttaccaatg taagcatcga tttgatttat gcattaccgg gacaaacact agaaggatat
      961 cgggatactt tggaaaaagc tttggctttg gatctgcctc actattcttt gtactcattg
     1021 atcctcgaaa acaaaacaat gttcatgaac tgggtacgac aaggaagact cgaactgcct
     1081 gatcaggaaa cggaaaccag aatgtttgaa gagacgattg aagcaatgga aaagcatgga
     1141 cgtcaccaat atgaaataag taactttggg ctctccggac atgaatctca acataacctc
     1201 atgtactgga acaatgacca ctattttggg tttggagctg gtgctagcgg ttatcttgga
     1261 gatattcgtt accgcaacaa aggaccgatc caacactatt tacgcccatt acgagcaaat
     1321 gaacgcccgg tattggaaga agaaaaattg acacgaaaaa atcaaataga agaagaaatg
     1381 ttcttaggac taagaaaaaa aacagggatt actaaaaaac atttctatga tcgctatcaa
     1441 aaaacaattg aatcggtgta cggtgatgtg ctaagggaat tagagcaaga agggttactt
     1501 gttaatgaag aagaccgtgt ttatttgaca ccaagaggga cttttttggg gaacaatgtg
     1561 tttgagcgct ttttatttga aaaatagttt gaagaatatt tgttttttat cgaaacttga
     1621 aaagaaaaag tttagaaagt ttgacaatga ggtattgaag tattgatata actggattta
     1681 catcaattat ttagaattag cactcttgtg tttagagtgc taaaaaatgg agtctttctc
     1741 ttgacaggag aagggcttct tgctatattt ataagtgaat tagcacttag aatatatgag
     1801 tgctaatgag aggtgaggaa gatgttaaca aagcgacaaa acgacatttt acgtctgatc
     1861 atccaacact atacgagtag tggcgtgccc gttggttcca aaacattaat gtctgaggga
     1921 gtcaaggcca gctcagcaac gattcgcaat gatatgaaag cattagaaga tgaaggattg
     1981 ctgttgaaga ctcactcttc ttctgggcgg attccatctg ctttaggcta tcgttattac
     2041 gtggatcatt tgttaaaacc tgctcgcgta gcagaagatg atttgctgct gattcgtcag
     2101 tctctaggca gagaatttca tgaaatcaat gagattatta agcaatctgc tgagatatta
     2161 tctgagctga caagctatac gactttttca ttgggacctg aaataaaaga ccgccgactg
     2221 actggctttc gtatcgtacc cttgaataat cgacagatta ttgctatcgt cgtaacagat
     2281 atggggaatg tagaaagcca agtgttcact cttcccgaat atatgggaag tcaggaacta
     2341 gaaaaaatgg tacagattgt aaacgaccgt ctagtcggag atcctctgat gacggtgtac
     2401 cataaacttc gtacggagat tccgatgatt cttcataaat atttccagac gacagaaggg
     2461 atgtctcagt tatttgacac cgttcttggt catgtttttg aagaaaaggt ttatgtcagt
     2521 ggacaaatga atctcttgga ttatgagccc cgtcaagatc ttgatcagtt caaatcgatg
     2581 ttttctttta tgaaagactc agacggactt acacaaatga tcactccgat ggatagcaat
     2641 attcatatca agattggtgc tgaattaggc aatgatttat tgcagaatat gagtatgatt
     2701 caagcaagtt acgagataga tggtcatgga agaggtacaa tagccttgtt agggcctact
     2761 agtatgcctt attctcggat gttcggtcta gtcgatgttt atcgaagaga actagcagct
     2821 aaacttgctg attattaccg ctcgcttgac ttgtcaaact cgtaatgtga ataacgaagc
     2881 tatttgcagc tgatcgttct ctctttctta gtaagagcaa tcaaacagct gactgaataa
     2941 tgaaaggaag actgtatcgt gaaagaaaat caagaagaac tagataaaga aatgacagat
     3001 gctcagccag aaccggaaat cgatgtcgaa gctgcagaag attcgggtat ttcagaagct
     3061 gaagcggaag aattcgaaac agcgaaatta aaagctgagt tagaagaaat ggaagataga
     3121 tacttgcggg ctcgtgctga aatcgcaaat atggcaaatc gaggaaaaaa tgagcgtgag
     3181 caattacaaa aataccgttc acaagattta gccaaaaaat tacttccatc aattgacaac
     3241 ctggaacgtg cattagccac agaagtgtca gatgatcaag gggcaggttt gaaaaaaggc
     3301 gtggaaatgg tcttggaaag tttgcgtaac gcactggaag aagaaggaat tgaaaaaatc
     3361 cctgctaaag gagaagcctt tgatcctaat cttcatcaag cagtacaaac tgttccagcg
     3421 actgaagata caccagccga tacgattgta gaagtactac aagaaggcta caaattacat
     3481 gatcgcgtgt taagaccaac tatggtcatt gttgcacaat aattgtgcgg atataaaact
     3541 tacactttca attaaagaag gagacgttaa ttatgagtaa aattatcggt attgacttag
     3601 gtacgactaa ctctgcagta gcagtattgg aaggaaacga agcaaaaatc atcgctaacc
     3661 cagaaggaaa ccgtacaaca ccttctgttg tatcattcaa aaatggagaa atccaagtag
     3721 gggaagttgc aaaacgtcaa gctgtgacaa acccaaatac gatttcatca atcaaacgcc
     3781 atatcggtga agcaggttac aaagtagatg tagaaggtaa atcatataca ccacaagaaa
     3841 tctcagcaat gatccttcaa tatatcaaag gattcgcaga agactattta ggtgaaaaag
     3901 tcgaaaaagc agttatcact gtgccagctt actttaatga tgcacaacgt caagcaacaa
     3961 aagatgctgg taagatcgct ggtctagaag tagaacgtat cgtcaatgaa ccaactgcag
     4021 ctgctcttgc ctatggttta gataaaacag atcgcgacga aaaaatctta gtattcgacc
     4081 ttggtggcgg tacttttgac gtatctatcc ttgaactagg agacggtgtc tttgatgtat
     4141 tgtcaacagc tggcgacaac catctaggtg gggacgactt tgataacaaa atcatcgact
     4201 acatggtagc agaattcaaa aaagaaaaca gcattgactt gtctcaagac aaaatggctt
     4261 tacaacgctt gaaagacgct gctgaaaaag cgaaaaaaga tttatctggc gtatcaagca
     4321 cacaaatcag cttgccattt atcacagcag gagaatctgg accattgcat ttggaaatga
     4381 cattgacacg tgcaaaattt gatgaattaa cagctgactt agtagaacgt acaaaagtac
     4441 cagtacgtca agcgttaaaa gatgcaggtc tgtcacaatc agaaatcgac gaagttattt
     4501 tagtcggtgg atctactcgt atccctgcag ttgtagaagc agtacgtaaa gaaacaggaa
     4561 aagaaccaaa caaatcagtt aaccctgatg aagtagttgc tatgggtgct gctatccaag
     4621 gtggcgtgat cactggtgat gtgaaagatg ttgttttact tgacgttaca ccattgtcat
     4681 taggtatcga aacaatgggt ggagtgttca ctaaattgat tgaccgcaat acaacgattc
     4741 caacaagtaa atcacaagta ttctcaactg cggcagataa tcaaccagca gtagacatcc
     4801 atgtattaca aggtgaacgc ccaatggctg ctgacaacaa aactctagga agattccaat
     4861 tgacagatat tccagcagct cctcgtggta ttccacaaat cgaagtaaca ttcgatattg
     4921 ataaaaacgg gattgtaaat gtatctgcga aagatctagg tacacaaaaa gaacaaaaaa
     4981 tcacgatcaa atcttcttca ggtctaacag atgaagaaat cgaacgtatg gtgaaagatg
     5041 cagaagcaaa cgctgaagca gataaagctc gtaaagagga agttgaccta cgtaatgatg
     5101 tagacgcatt gctattctct gtggacaaaa cattgaaaga attagaaggc aaagtggacg
     5161 aagaagaagt gaaaaaagca gaaacagctc gtgacgaatt aaaagccgct gttgaagcaa
     5221 acaacatcga ggaaatgaaa actaaacgcg atgcattgaa cgaaatcgtt caaaacttga
     5281 ctgttaaact atacgaacaa gctgcacaac aacaagctca agaaaatcca gaagcagctc
     5341 aaggcggagc agatgatgtc gttgatgctg attttgaaga agtcgatggc gacgataaat
     5401 aatcagttca ctgaatttcg agagaaggcc aaagttgcta ctttggcttt ttcttttgaa
     5461 aaatagtttg aagtatcggt ttttgtttca aacaaaatat gatatgataa caagcgacgt
     5521 gaaaatgatg agaaccaata ggaggaaagc ctatggcaac aaaacgtgat tattatgaag
     5581 tcttagggct ctcaaaagga gcatcagaag atgaaataaa gaaagcttat cgtaagcttt
     5641 cgaaaaaata ccatcctgac atcaataaag aacctgatgc ggaagaaaaa ttcaaagaag
     5701 tatcagaagc atatgagatt ttaagtgacc cgcaaaaacg cgctgcatac gaccaatacg
     5761 ggcatgcagg cacagatcca aattacggtg ctggtggagc tggtggcgga tttggtggat
     5821 tcggcggctt ctctggtggc ggcttcggtg gttttgaaga tatcttcgac tctttctttg
     5881 gaggaggcgg tcgatcagtc gatccaaatg cgcctcgtca aggtgctgat ttgcaatata
     5941 cggtcgactt gtcttttgaa gaagcgattt ttggtgtaga aaaagaaatc aagtataatc
     6001 gggaagaaat ctgtcatact tgtggtggaa acggtgcaaa accaggcaca caaccaacta
     6061 cttgtcataa atgtcatggt gcaggtacga tcaatgtaga acgccaaacg ccacttggac
     6121 gagtgatgag ccgtcaaact tgtgatgttt gtcatggcac agggaaagaa atcaaagaac
     6181 cttgtccaac ctgtcatggc tcaggacatg agaagaaagc acattcagtt aaagtgaatg
     6241 taccagctgg tgtagaagaa ggacaacaaa tgcgtttagc tggtcaagga gaagctggag
     6301 aaaacggcgg accatttggt gatttgtatg tggtctttcg tgtagaagaa agtgatatct
     6361 ttgatcgtga aggttctgaa atttactatg aattgccttt aagttttgta caagcagctt
     6421 tgggggatga agtccaagta ccaactgtcc atggagatgt gaaattgaag atccctgctg
     6481 gaacacagac aggtacgaac ttccgtttac gtggaaaagg cgctccgaag ttacgtggtg
     6541 gtgcaacagg cgaccaacat gtcaaagtaa aactgatcac accgaaaaat ctaaatgaac
     6601 aacaaagaga agcattacgt gcattcgctg aagctggcgg catcaaagtt gaagaacagc
     6661 aagaagacgg tttcttcgat aagtttaaag atgcttttgg cgggaaaaag cggaagtagt
     6721 ttttctagta aaaatataaa gtagacttgc ccaaattttc tatttctagt aaaaacgcta
     6781 agtcattaat aattaaaaac cagacgaaca atcatacgtt cgcctggttt ttttgtagtt
     6841 gtcatgtttg taccaacaaa aaagccagct ttagaataag ataatcatca tagtttctga
     6901 aggtggtatc gactatgtac ttgatatcgt caatgaaccg atggtacttt cttatgcgat
     6961 ggaacaggta gagcgattgc ttgtttgtgc aggagaacaa gcgatgcggc tggtacagtt
     7021 aagtgaaaaa acaagaaact aaaaatgccc ggtcgtcatg aagcaaccgg gcatttttgg
     7081 tttttattaa atattcaata ctttattcaa gaaatctttt gtccgttcat tttgcggatt
     7141 ttcaaagatt tgttgaggcg ttccgtcttc taaaaagttt ccgtcgtcaa tgaacatgac
     7201 acgattggcc acttctttag cgaagcccat ttcgtgcgta acgataacca tcgtcatacc
     7261 ttgttctgct aactttttca tgacgttcaa gacatcaccg accatttcgg ggtcgagtgc
     7321 agatgtaggc tcatcaaaaa gcatgatgtc agggttcatc gctagagcac gagcaatagc
     7381 cacacgctgt ttttgtccac cagaaagcat ttcaggatac atatcttttt tatcttctag
     7441 accaactgtc tgcagtaatt tgattgcttt tttctctgct tcttctctag attctttctt
     7501 caggtccatc ggtgctaaaa tgatgttttc caaaatagaa agatgaggga ataggttaaa
     7561 gtgctggaat accattccga tatgttgtct caccaagttg atatctgtgc ttttatccat
     7621 caagtgggcg ccgtcaataa taatttcacc gtcagaaggt tcttctaaac gattcaagca
     7681 acgcaaaaat gtactttttc ctgaacctga agggccaatg atacagacga catctccttc
     7741 gttgatggat acattgatgt cgtttaatac tgtgttatcg ccgtattttt tgactaaatg
     7801 ctcaactaag attttttctg ccatcttact tcaccttctt ttctaaaact tttgctaatt
     7861 ttgtcaatgc tgtgatcaaa atcaagtaca tgatcgcaat gatcaagtaa acatacgtac
     7921 tttgtgttgt acgtgcaaca atgatcttac cagtttgcag caattcgact acaccgattg
     7981 cggaaatgat cgttgtgtct tttaatgaga taacgaactg attgacgaat gatgggatca
     8041 tgatgcgtat tgcttgtggc aaaatgattt tttgcattgt ccgattataa gaaagaccta
     8101 aacttctgga agcttccatt tgtccgacag gaacggcgtt gatcccgcct cgaacaatct
     8161 ctgcgatata agcacttgcg ttaagagtca aggtgataac accagcaagg aagtcaggaa
     8221 tcgtaaatcc aacgatacca ggcaacccaa agaaaataaa gaacgcaaga accatcattg
     8281 gaattcctcg gatgatatcg acataaatac tagcaattgt gcgaagagcg cgtattggtg
     8341 ctacactgaa taatccaaaa atcacaccga ttactatagc caaagcaaag gagatcaaag
     8401 ttaggagaat tgttctccaa agtcctttca acaatgcttg atagttgttt tggataagtc
     8461 caagaaatgt tgattcatca gcagtggaag actcactaga agtttctgaa ccatccgcta
     8521 catattcttt gataatctta tcgtattcgc ctgttctttt taattctttc aatccttcat
     8581 tgaacatatt aagaagctca ggattttgtc cttttttaac ggcaaatgca tattttccgc
     8641 cagattcttt ttcgatcggt gtttccaatt cttgtccttg tttgatcgca taaccgatta
     8701 ccggataatc atccatcatt gcatcgattg cgccaacttt taacccgtcg tacaattgat
     8761 cagcagcatc aaaatatttg atagaatagc catattcaga ttcatggtcg cttaaaaatt
     8821 cagcactttc ggtaccaact tttgctccga ctgtttttcc tttcaaatct tcataagact
     8881 tgatcgaagt atctcctttt ttgacagcta attggatccc gctatcaaag taagaatcag
     8941 aaaaatcata gcttttcttt cgttcatctg ttacagtcat tccggcaatc attccgtctg
     9001 cttgtcctga ttccaaagct tgcaaagcag cagagaaacc acggtagtcc attcggatgt
     9061 taaatccttg cagtttggcg atgttttcca tcaattttac atcgatgcct tcgtactcgc
     9121 cctttgcgtt ttgaaattca aatggagcaa aagcagagtc actggcaatt acgtatacgt
     9181 cttttttggg ctctattttt tgcattgtct cgcttgtgct tgactctgcc gttgtagtgt
     9241 tgtcagcttg tgcaacatga ccaaacgcaa gtgtcacaag tcctgtcatt aagaaaacaa
     9301 atgagtgaaa taaatgcttt cttttcataa tcaacctcct aaatttccaa tattttatta
     9361 ttataggcga aaagtgctga atcagacaag ac
//
LOCUS       DAKBVG010000104         9326 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000104 DAKBVG010000000
VERSION     DAKBVG010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9326
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.104"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(11..1375)
                     /locus_tag="OUD09_001745"
     CDS             complement(11..1375)
                     /locus_tag="OUD09_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCT9693012.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(1372..2931)
                     /locus_tag="OUD09_001746"
     CDS             complement(1372..2931)
                     /locus_tag="OUD09_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCT9693013.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(2987..5467)
                     /locus_tag="OUD09_001747"
     CDS             complement(2987..5467)
                     /locus_tag="OUD09_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693014.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(5451..5786)
                     /locus_tag="OUD09_001748"
     CDS             complement(5451..5786)
                     /locus_tag="OUD09_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693015.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(5800..6834)
                     /locus_tag="OUD09_001749"
     CDS             complement(5800..6834)
                     /locus_tag="OUD09_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HCT9693016.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(6924..7604)
                     /locus_tag="OUD09_001750"
     CDS             complement(6924..7604)
                     /locus_tag="OUD09_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HCT9693017.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            complement(7888..8991)
                     /locus_tag="OUD09_001751"
     CDS             complement(7888..8991)
                     /locus_tag="OUD09_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HCT9693018.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
BASE COUNT         2676 a         1902 c         1663 g         3085 t
ORIGIN      
        1 gtgatatttc tcacagtctt ttatctactc tattgctatt tgctctctta gctagtactt
       61 taaaagctaa ctcattgagg tagtgaaaac gagaggtgtg ttcagacaga aatccaaaat
      121 cctcaactgc tagctgtgga atcgtagttt tgtgtttcac gatatccgca agcaggttca
      181 aatctgataa taagaattct tggcttatca gctgtccgcc aattatttct aatgtatccg
      241 gatggtagat cagctttgca tttactgttt tgttttcttc atcgtattgt ggaaatagat
      301 caaattctcc tgaacacgaa ccggtatttt gttccaagaa tggcgcttca tcttctgtca
      361 gccctacagt tcctaaaaag tagtctataa tgactgctgc tgttatcttt tgtctgaggt
      421 cgtaagcggt tggctgacca ctcaaagtta gcgctactgc tcttgccata tgtacagctc
      481 gagtaactaa aggtaaaaag agatgccgtt tatttttgtc aaaactgact gggacgatat
      541 ctccaatcgc atagatgtcc ggatcacttg tctgcagata ttcattgact atcacagtct
      601 catcctcata caaagccact ttttctttta acaatgtcgt attaggacga gtttgggcag
      661 aaaaaatgac catatctgcc tttattgctt gagatagaag ttgtacggat actactttct
      721 cttctgattt tttccaatcg atcaagaatt catttggata aaaattgatt tgcttagttt
      781 gcagctgatt ttgaaaactg ttgatcatat ccttatcgaa atatcttgat aaaacattcg
      841 gcatataatc aactagatga ataatgtatc ctttatttct caaagcatct gctaattcca
      901 atccaatata accagcaccg aagatcacta cttcttttgc ttcttttaaa gcggcaagta
      961 cttgcttaga ttgcactaga tttttatagg tgtagacatt ttcagcttca atttgtttat
     1021 acaaggtgac aggagaggaa cctgttgcta cgattaattt atcataggtt atggtctcgt
     1081 tttttccttc tttggatgcg aggatgagct gatgtttgtc tggatcaagt tcagttactc
     1141 tccattcggt gatcagtttt gccccacttt cttgaagagt ccgcatgtta tgggcaactt
     1201 cagctaaact acgtatcttt ccttttaaat aaaggttgat tccatttgaa acatagccta
     1261 gtccatcttt atgatcttca tcgatcagta aaacttctgc tttcggttga agttttctta
     1321 gatttagggc agctgtgatg cctgcatggg aagcaccaat gatcacgatt ttcatgtttt
     1381 attcatcctt aaattcaatt tttctaaata acattctttt aaaaattggt tcaaataggg
     1441 gaaatcataa ttatattcgt tgctagtgaa tacaaaaact ttagcggact tctgtgacgg
     1501 gtactctttc aattggctag tcaaaatgat atcatactgc ttttctttct cgtattcgac
     1561 taaatcatat ttgatcgtat tcagcaattg ttcctggatg atttctttca cgatatccgc
     1621 cattagcctt tcataaggaa gatcataagc aatttgcaga cgtaattcta actgcttgat
     1681 cagcgtccgc aaaatagatg catagcgagc aaataattca aagcgggcat tttcctcgtc
     1741 tacttcagta aaatcaacaa tagcaaatgt ttgtctgacc aatctttcac ataaaaccag
     1801 gaattgttta tcttctactt cagttaaccg ctgttttaat ccttcttggc caaaaatatt
     1861 catccatcct ctaaaatagg caaaatgagc gtgattttgc atcactgtca acgcaatctt
     1921 ttgcgaaatc tcttctggaa aaacataaaa agagaaatat tcttctatcg attgtttaaa
     1981 cgttcgattc aattgagcta ctacgtcgtc tttgtatttt tcgcttatca aaaactcctg
     2041 gacatgcgag ttatgtgtat ctaatgtaaa gttactgatc atgaaaagat aaaaaaccaa
     2101 cgtttcattt tgattccaag gaaaggcata ttgaaagaag taacgggtca aaaatgtatg
     2161 gaggtccaat aaaaattgtg tcgtttgaaa caatccaaca ggaagattgc tataatctgg
     2221 tttggatttt ttcgtacagc gcattcgctt cttcataatg gaaagccata tttcaaattt
     2281 caatcgtcct tcttctgtaa tagtaaaact aaactctttt tgcagcagat ctaaaaaagt
     2341 gtttcctgat aaatcactgt attctttttt gttttcaaga taaggacgtc caaaccaaaa
     2401 taattggaaa taaaaataac aaatttgact ttctggaccg gtcagccgcc cgctttttat
     2461 tttcagatga aactcattta aagaatcatt taattccttc actctgcgag acaaagtagc
     2521 cgaactgata ttaagttcca tgacaagttt ctgccgatca aatgagccat tttccaacag
     2581 ataaagaagt atctgatatt ttgtagaata gcgcaagtag caatgataat aaacatcgac
     2641 tgctgctata tttccatgtc tgattaataa aatattccct tcttggataa caatttccaa
     2701 ttgtttgata ttttccaagt tagcttgtaa ataaaacaga tcttctttca aggtagcccg
     2761 atcaactgat aagcgggtaa gtacgttatt aacggaaatc tctccttttt cctctaataa
     2821 aagttggaaa agtttgattt gccgttgttc atttttttca agcaaactga aaagttccaa
     2881 gtccactcct ccaagcattt ctgattttaa cagcagaagt ttccttctca tccttgaaaa
     2941 agaggatctg tgacagttat cacagatcca tacaaattct aatcacttag ctggttggcc
     3001 cagctgtcag ttcccatgtg accatgcctt gatacgttgc gccaggttga gcattcgtac
     3061 tgctatttac ctctaagagt aaaccatcgg tatcgggaaa gtcaacgaga acctctcctt
     3121 tagctgttcc atcactctca taaatcaacg tgttttgacc ttgctgaatc aattgttttg
     3181 atccagaatg attgaagtat aattttgttc cgcttaattc ttgaccttga tcattcttaa
     3241 ataaagaagt caatgtccct gtcagttgcc aaggagacgt gcttggaagc cgagtatccc
     3301 gcacatcaaa agaaaaatcg ccttttgctt cataataact tgtcttcaac ggtttgggga
     3361 gatcgttacc aaatgtaaaa tcatctggta cattaactaa tgttaattct tgtttgggga
     3421 caagaattgt tttagcagat gtttggacaa ctgggttagg aagcttagca ccatctgcta
     3481 atgtaatatt gctggaaagt acagctgcct ttgtcagtat gctgacggat tgtacagaag
     3541 tatctacagt atttaattga tagttgtaag taagatgaat ggtatctcct attttgctca
     3601 atagatctgc tgggacagaa actccttgtt gaatgccggt atctgcgata gttgtaccat
     3661 ttaattttaa ggaaccattg atataagtag tattttcagg aagtgagatc gtgatttttt
     3721 tgtcggttat cgcatcccca gattttgtta acgtgaatgt cacattttct tgaatcgtat
     3781 ctcctgatag agcagacagg ggatctcctg tctcaggttg tctgctaagg ttttttagct
     3841 gactatctgc actcacagat ggaaaattag ccactgttat ttgaggaaga gtggtgattc
     3901 cagcttgttc agactgccat ttgtacgcaa aagacggata catgttagta agtacatcgt
     3961 ccaccaagcg ataatcaaaa taatattgcc ctgaagccaa tgcaggtagt gtagaagtag
     4021 ctgtatttac acccccgtta tttgattgga tcgtttggaa aagttgatct gtccatcctg
     4081 tcgctaattt ttgggttcct ttcgaataaa gcctgtaatg gatcgaaccc ttttttagtt
     4141 cagttgctga ccaattggcc gtgacttggt acgatgcaga agaaaggagc tttccccact
     4201 gactatcggc tgttaagttt gttggtaaaa ccgttccgtc tagcttgtac tgctccaata
     4261 cctttaaagc aacaggagaa agcaatccgc cttgcgcatc tttcccactg tacacgatat
     4321 tggctgtctg attcaatcca cttgtgcttg ctggagtatg agtcagtatt gctccttgtg
     4381 taacgttttt tcctgtaaaa tagttgacta cgcgatcaaa gcgactaaat ggcaactgat
     4441 caaaacgtcc ttcagtcagc cccatcgtca atgtccgact attggtagag cctggaaggt
     4501 tctggttatt tccatccacg tattctgtgg tatcagcaaa tgtattatat tttcctgttg
     4561 aatcacttgt tgagatgtaa ctattccacg tatttatctc tgcacttgca gaaaatgcat
     4621 atgctcctgg attcaagttt ccgccagtta atttaggata aaatctacct gcagctggag
     4681 ctaaaatcga attgaacata cgatcttgga taatattatt cgaatcataa aaatcaacag
     4741 aaaaaacggc ctttttagac atagaaggtt gtaatttacc cttggtcaaa attttgtttg
     4801 gactcgtatc atatttatct ccgttttctg tcatcttcgc gacattagct gtaacatcaa
     4861 attgaagcgg gatatcgtcc acgttgaatt cgcctcgcgt aataatagca ataatatctg
     4921 ccaatagagt atttgtatcc cacgacccca tcccaaaacg caaggtatgg cttcctgctg
     4981 ataataaact accgctagta accttgatgg gggcaccggc aatatacaag taggcgttgt
     5041 tccagtcaat tgcttttaat acatcactgc ttgatacatg ttccggcaaa ttgatgatcg
     5101 ccacaaatgc aaatccttgt gcagtgatat tcaccaaatc aagtacattt gttagtttca
     5161 catggctttc ccaatgaaaa cggaccgtgc cacctttcca aggatcattt gaaccatcgt
     5221 tcaaataagt agcagtcaat ttaggcaatt ggttagcccc agttagttgg acaagcttcc
     5281 cgccattgac tgcactacgt ccaccatttg gaggtacgat attttcatac gtgtatggat
     5341 cataagaaat ggcaggaggc gttgctccaa cagcttctgc cgaaggtgaa aagataaata
     5401 tacttaaaac aaaaagcgaa gtaaaaaaga aggagcgaaa aaatgaaaat ttattttgta
     5461 gcaacatgtt catcctcctt ttcctttctt ctccataaca acaaccacgc aagaagccat
     5521 aattctatac caatgatcaa ataaagaaaa cggatctttt cttcattcgt ctccgggaat
     5581 acgccatgtt gattagattc tttataaatc ggtagctgtg tctgctgttt tgatgcgcca
     5641 ttgactgaac cacttggatg cggaactgga cttagatcat aaaatgtaat cgtacctgaa
     5701 cttttggtat ctgcttggac tggctcttgc cagctgaaga acagcaaact tgcaataatc
     5761 cctcccaata agatcgatgt tttcaacgaa atcacctcat catgttgttt ctcctttttt
     5821 ctttttcttg tggacccaca aaaacagcaa aatcaaccat aacactagaa ctgcagcaac
     5881 caatcctgca taaagccatg ggaaagattg ttcttgtact gcaaaaacgg aattttcatt
     5941 tactttttgt gctttttctt ttttgatcgt aaagttttgt gtaaatcgcc attcattact
     6001 gtcgcctgat tttgctatga tttctgcgcg gtaatcgccg gcttttgctt tatcaccagc
     6061 taaatcaaga ttcaagtgaa aattcgaatt tggtgcaact aaaaattcat tagacgtatt
     6121 ttcaatataa agctgatttt cataaaagat cttgctcgta aatatcaaat tgctgataat
     6181 cctaggctga ggattttgga aacgaagctg aacagttggc tttgcgttga cctcgtccaa
     6241 atctcctgat aaaagtttca gatccggttc tatcgtctct tgactttctt gtaaaacgac
     6301 tgcgagatta taagcaatac gatttttgat ttgtgctcct tctgtagcat tttctgtttc
     6361 agcttcttct ttaggtgaaa catgaatacc cgctaataca cgtcccttaa agctttttgc
     6421 tgggattttc acttctatag gtatagttgt ttctgcatgt gctgatacat tgatttcctt
     6481 tttcgcaatg agcaaaacgt cacggacatc aaacggcaat gtctcatcta acgttgggtt
     6541 tttttgacta taatcgatcg ttccaccatc actcgttacc gccggattga cagagacctc
     6601 aaatgttcgt tcttcatctg aagaattgct gataattact tctagtgtct gattttgttc
     6661 cggtgccact aataaatcaa aataaccgat tgctttatct atttggttct ctggaaatac
     6721 cggtttgatt ccaaagtctc ctgctgaatc ctttgcttgg ctgattgacg gaaaaatgaa
     6781 aaatagactt aaaatagaaa aaaacgaaac aaacagtcgt ttactttgat tcattgtcca
     6841 ccacatcctt tcaacaacaa ctgcggtaaa aaatccttca cctatagtat tttcaccgca
     6901 gtttgttgaa tttatttcat actctatgct actgggccag ccacgaggtt ccaagtcaaa
     6961 tcagccgtga atgttccagc tgctgctttc ggcactttca gtgtgatatt tgctggatca
     7021 taaacagaaa gccatgttcc cagaccttga tcttttgctg ctgtaaatat tggtgtcgct
     7081 gcacttgctt tactcaaccc agttacagtt tgactagttg gtccagtgga ttcgttatct
     7141 gacgtacttt taacagtgga tgttccaaaa tctaattcag cgccttgcaa aacttgtgaa
     7201 ccgtttacaa aatctgaaac cgaaactgtc acgttccacc cttgtgcttg gccatctgca
     7261 cctacacctc gacgatctga tacttggaga taaggtgtat cgttttttga agcgtctact
     7321 tgatacgttt ttgttccgct ttcaatctcg tgtgtaccaa atggcaattc aggaactaca
     7381 tccaatgtta aagaacctgt ctggccagtt gctggatttt ccggatctgc tggctctact
     7441 ggttttgaag gatcagtagg gttaagaggc tcaacggtat ttgttggttc agtaaaggta
     7501 atatctcctt ttgaagtcgc ttgaccctca gcaaaagctg gtgttgccaa gccgcctaga
     7561 agaagtgttg aaagtaataa tgtcccaatc atgttctttt tcataataaa tcactccttt
     7621 ttcttagtga cctaagtata gtagagacag tatgcaaaaa atcctcatga attcatccgc
     7681 ttacttgaaa ttatgaccct ttttgtataa aagagaaaaa aacaataaaa tcgtttacat
     7741 gaatattctt ccttttttat taagtatgaa acaatatttc aattttattt tttcgtttca
     7801 aattattcta ttagtgacat ccttgaacta taaaaatagg agtacggcat catctgccgc
     7861 actcctattt ttttcgtcat ttatttctta aataaaacga ttctcacgca attttatttt
     7921 tttgatatct tctactgttt gtgttccagc taattgcatg accatttcca attctgtttt
     7981 aaagaaatca aacacttgat ggactcctgt acttcctcct aaagacaatc cataaataac
     8041 gggtctgcca atagcaacaa gatcggcacc tgatgcgatt gctttgaaaa catgttgtcc
     8101 acgtcgtaca ccgctgtcaa acacgattgg tacacgttta tctactgctt ctgccacata
     8161 ttgcaacgaa tcaaacgcag caggcccccc atctaattga cggccaccat ggttagatac
     8221 ccaaattcca ccagcaccag attctaatga tcgatagaca tcttcttctg attgaacacc
     8281 tttcacgtag actggcaaat cagaatgagc agcgataaat tctacatctt ttggactaag
     8341 tttttgtttg gaagatttat atacagcatc catcgtttgg cctactcctg attgataagc
     8401 ctgaacgatc ggcattggca gcgggaaggt aaacccgttt ctgcggtctg tttcacggtt
     8461 tccgccaact gttgcatccg ctgtcaatac gattgctttt gctccattac gcttggccac
     8521 ttcaagaata tccaaattga ttccattatc tttgctcata taaaattgaa accattgcgg
     8581 tgctttctcc ccgcctgctt ctcttatctc ttctaaagta caagaagcgt aagaacttgc
     8641 tgtatagatc gtcccaaaat ctgctacacc ttttgcagaa gctttctccg ccttcacatg
     8701 agctaatcca tgagcagcaa ctggtgccat gatgattggt gcagtcagca tttcttcgtc
     8761 aaaatgtgta gttgtatcgg gtaattctac atctcttaat acatgaggaa taatcaaacg
     8821 atgattgaat gcccgttcat tttcttgata agtaaataag tcgccagcac cactgctgat
     8881 gtaaccataa ccaccagctg gaatcacttg tgaagccgcg atttctaaat cttccatatt
     8941 gataaaatct actattcctt catttgtacc tgcttgatat gttttttcca tctcaatgcc
     9001 ttctctctat aaaatttgaa tgatgaacaa tatgatagcg tttacacttt ttatattact
     9061 cttcgacatt gatacaggca aataatctgt ttacatttca gccattccag atgtcactac
     9121 tctggaagaa atgattgtaa taaaaataga taaaaaaccg atgggacttt gacaaaagta
     9181 gtgtcaaagt cccttttttt gaataaacgg tgttcaaaag ccattttctc ggtattaagt
     9241 caaatttatg caaaaatgtg gaaagccatt ttcacaaaat ttttcttact actcgaaact
     9301 aagcgacttt ttcacaacct ctcgat
//
LOCUS       DAKBVG010000105         9319 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000105 DAKBVG010000000
VERSION     DAKBVG010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9319
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.105"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(<1..269)
                     /locus_tag="OUD09_001752"
     CDS             complement(<1..269)
                     /locus_tag="OUD09_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCT9693019.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDFVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFNQETKHLTGREKRLIKRNALSFLFQ"
     gene            complement(273..1043)
                     /locus_tag="OUD09_001753"
     CDS             complement(273..1043)
                     /locus_tag="OUD09_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HCT9693020.1"
                     /translation="MIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQE
                     ISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSTYWERNILSGEIYPPLK
                     IKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFS
                     FFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIER
                     EPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            complement(1461..1877)
                     /locus_tag="OUD09_001754"
     CDS             complement(1461..1877)
                     /locus_tag="OUD09_001754"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693021.1"
                     /translation="MMTREKEAYERFILLFVERFNQAFHTDYATNIFEDFENSYSYSV
                     ISSTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNGFSLHQVLWCLLGKKKI
                     IITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGI"
     gene            complement(1928..2309)
                     /locus_tag="OUD09_001755"
                     /pseudo
     CDS             complement(1928..2309)
                     /locus_tag="OUD09_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2435..3100
                     /locus_tag="OUD09_001756"
     CDS             2435..3100
                     /locus_tag="OUD09_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCT9693022.1"
                     /translation="MNIGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHQTLGLPIDFPSIESATESIEPTLPDEPLVIKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETVDRFNPFLKPQIGYTTLPVGYVENSIEHWVCNDIFVHGTW
                     LSFHAGEVEKGKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILIQDYDDALKRSPQEP
                     DRH"
     gene            complement(3497..5530)
                     /gene="brxL"
                     /locus_tag="OUD09_001757"
     CDS             complement(3497..5530)
                     /gene="brxL"
                     /locus_tag="OUD09_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease Lon-related BREX system protein BrxL"
                     /protein_id="HCT9693023.1"
                     /translation="MEQQEILKLSEFFPGKIVRKDLTKKIKEGANVPVYVLEYLLGMY
                     ASSLDETLVEKGVENVKNILARNYVRPDEAEKVKAKIRELGGYTVIDKVTVHLNEKKD
                     CYEAEFSNLGLKNVQISEEYPKKFDRLLGGGIWCIVSMEYWYDENDRWNSPFIAKKVD
                     PIQMPGLDIEAYKMARKEFTTNEWIDLLLRSIGMEPTQFNKRVKWLHLARLIPLIENN
                     FNLCELGPRGTGKSHVYKEISPNSILVSGGQTTVANLFYNMASRTMGLVGMWDCVAFD
                     EVAGIKFKDNDGVQIMKDYMASGSFARGKEEKNASASMAFVGNINQSVDVILKTSNLF
                     EPFPEVMGTDSAFLDRMHCYLPGWEIPKYRPDFFTNDYGFITDYFSEVMRELRKVSFG
                     DSFDKYFKLGSQLNQRDTIAVRRIVSGMMKLLYPDGRFEKSDVEEILRFALEMRRRVK
                     EQLKKIGGMEFYDVNFSYIDLEEEREEYVSVPEQGGGTLIPEGLGKPGHLYTVSYGKT
                     GQLGTYKLETQMTSGNGKFERTGINSDAGAREAMDSAYRYLRANYGSVSQSISLTTKD
                     FVVHVQDMNGIGMTKTLSLSTIIALCSVSLNKPVLPSLVILGDITIGGSMIKVDNLAN
                     CLQVAKDAGAKKVLVPMISAADIGTVPPELMGTFNLIFYQTPEDAVFKALGVE"
     gene            complement(5648..8170)
                     /gene="pglZ"
                     /locus_tag="OUD09_001758"
     CDS             complement(5648..8170)
                     /gene="pglZ"
                     /locus_tag="OUD09_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type A"
                     /protein_id="HCT9693024.1"
                     /translation="MTDINTQQIEEKLNQRFPNYGCRKIIFWFDSNQDFLEEIDSLVI
                     QNAKIYRLEKDAQFKTKRLLEYEDPETNYLIYAPFPRWDDKDEDNHLLSVLNYSEEFS
                     ADRLAIIMNELAIPLRFHQTVKQYRNFFNAKDRMRSFGEFSTHMTINKSETIELMIMA
                     VLLKSKSIRLNDLLRVVLKSFTESRENPLRELDKYQLTAKFWEFIQMKFGFTDSQPTL
                     EKLTMCLYLNYFYYQIDQEMPKKFKGFEILHLKANIITFMDQFMNDTRYMESFEQLSK
                     KVYQAIDGDGLLDSIEIDDLIEADVFSEIHAKILMFYAERLKASDTTTKIAGQTIFDS
                     LKQREKMHFASDYIYEYQLLKHAYYLVDKNRLCLPMNMDNLLTQYEKEMYWVDTHYRK
                     FIWNSDHIEEKQKYGQLYRLVEANYSKFLDQSGQIWNQTFEFKNRPSIRKFYNQAVMK
                     RSVKTVVIISDAFRYELGMNLERKLVREKKYSTNMASFLSVLPSVTEFGKAALLPNNQ
                     LTYNEGTEVLIDGKKTNGLSNRNAILQQRDANAVALNYDDVVKMNKAEVREVFNGKSV
                     IYIYHDQVDRTGDHSNENRVFEACDLAISEISRLINRLHNDGSIYRFIVTADHGFIYK
                     RSHVEEVDKIENPSRNEKDRVERRFILSEEEYEIIGTKNYSLGDSLGNDDTRNICVPI
                     TSSIFKKAGGGQSYVHGGSSIQEMLVPVLEITVAKGSNAKEPVAIELMTSNRRITGLS
                     TTLEFYQKNAISDVYDPASFWLYFEDEYGERISNEEIYVADSKTEDANQRFTKFVFEF
                     VNRRYTVEDTYYLVIKNQKNDLTERIKFIIDNPFAESFDFDI"
     gene            complement(8286..9230)
                     /locus_tag="OUD09_001759"
     CDS             complement(8286..9230)
                     /locus_tag="OUD09_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002397590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693025.1"
                     /translation="MFKKDLSEAFNCLSRQLVNQFNTLKSNEEELNDIFINHYSLEKE
                     LTKEVQDEYISARLPDKAREIKDFISYSIGCIFGRYSLDEDGLIFAGGEWDPSRYQKF
                     KPDTDNVLLITDDEYFKDSQIDIVNRFIDFVEITFGKETLEENLQFIADTLGGKGTSR
                     EIIRRYFVQDFYKDHLKTYSNRPIYWQLDSGKQNGFKALMYLHRYTPDQLGKVRTEYL
                     HELQKAYDNRIELRQQQISTSDNPKERNAMTKEIVKIQKQLKEVREYDEKLGHLALKR
                     IELDLDDGVIVNYDKLQRDPKTGEKFQIFAKGVKEPKK"
BASE COUNT         3048 a         1796 c         1343 g         3132 t
ORIGIN      
        1 tcttgaaaaa gaaaagagag ggcatttctt tttatcaagc gcttctctct tccagttaaa
       61 tgttttgttt cttgattaaa taaataaaat tgccctgaac tgaattgatc attcaatcct
      121 aatatattca ataaagtact tttccctgct ccgctctctc caactacagt cacaaaatcc
      181 ccagcatccg cagtgaaacg atccaaatga agaatcgttt tgtttcgaat gatttttttc
      241 gcacctttta tcactatcaa cggctccaat tgttaacctc cattcatcag caataactga
      301 cttttctttt ctgtagacaa aataaaaaca actgaaagaa taaaatcaat gaagctaaag
      361 aaaaaaatat taaaaagata tttaggctct ctttctataa gaaaaaggaa gattacactt
      421 gtcgttcccc atttgatgag gttcatcatg aagaaatctt tatacgtttc aaagagcgaa
      481 taaccacttg aacgaaagat agcgattttc cgacaattgt agacaaaata tatgcataac
      541 atgacatagc tcaacaagaa gaaagaaaag aaagtgagta aaaatatctg tatcaagtaa
      601 tgaaatgatc tgcttaacac tttgacagct atctcttcag tctgagtata cggaaatgat
      661 aactgcaagt tatcacgtaa ctgactttct tcaatcagct caaataacct ttctttcgac
      721 aaagagccta tcttgatctt taacggtgga tagatttctc cgctgagaat atttctttcc
      781 caatacgtac tgtttttcaa tgtcagtatt tcaacaaccg gataatgttc gatccagggg
      841 atagaaggaa taaatgtata aatgggttga ttgtttttta taaatagaaa tttcggttct
      901 ttttcactga tttcttgata atattctgtt atttgcgcca ggtcattttt taaatctgta
      961 tgtctatatc gttcagggat caatataaca gggtgcgttt ctttttcggt tatttggatc
     1021 aaccttccag agacatcaat caaaggaaat ttcttaagat aattcagatt tatttttatc
     1081 cctctgccaa actgttcatt ttcttcattc aagtaatctg atacattgac taatatactt
     1141 ccgtttttat tcaaagtttc atataattcc tgatcggctg ctgatgcaaa acgattggaa
     1201 taaataaaat ccacgtggtt tttcccgatg gttactggat aaaatacatg atagtcctca
     1261 ccagcgattt cttttggtgt ttcatgggaa acagctaact gtgaagaaga aaaaacgata
     1321 gttgctaatt cgacattggc aacggtcaaa agaagagaaa acaacaatcc aaatgtaaaa
     1381 agaatgtatt ggatatagtt gaacgcttct cttaacgaag cgattttttc aactaataaa
     1441 agagaaatca aagagatacc ctatattccg ctgaacaaga aaacgtacat ctggaacaaa
     1501 taaagaaatt gctccttata atacataaaa ataaaaccac caatgattcc tgcaattatg
     1561 aatgtaataa tgattttctt tttccccaat aaacaccaca atacttgatg taatgaaaag
     1621 ccattattca acaataaact gatttcttta ttcatcaata agaaaaagga tgataacaaa
     1681 aggaataaaa gaatcaatcc aatagttaaa tacactcgat aattatatac gtctgtactt
     1741 gaaataaccg aatacgaata gctattttca aagtcttcaa aaatattagt ggcataatca
     1801 gtgtgaaatg cttgattgaa tcgttccaca aatagtaaaa tgaaccgttc ataggcttct
     1861 ttttctctcg tcatcaagaa aaattcacct tcaaaatctt ttattttgat cgattcttca
     1921 atggacgtta cgcgaaagga ggtattagaa tagggtacct tctcgtgaga agatttgaca
     1981 tccccttgat aatacaatgt catttcaagc tttggctgta tcttgaagtt ccaagatgta
     2041 tctacttcat tggattcatt tactgttttt ttcatataag aaagctcagt ttctgatgag
     2101 acattttcaa ataccatgat tagctgctca tattcttctt gcccaataaa atgttgtggc
     2161 aatcgaatcg gagtataagt atctgtttga tggaaaaatt catttgtccg atagagtacc
     2221 tgtttattta taataaccaa ggcaaaacca ctcatgatac cggtcactat gattgtgagt
     2281 aaaactgtga actttttttt gattatcacc actctccctt acttaaatga ttctttgata
     2341 aaatcatacc gtttttcatc aaaaaaacgt atctatcaaa tgtataggtc atgtagaata
     2401 aatctatata ctgtagaatc gaaaggagaa gaagatgaac atcggagaaa aaatacgaac
     2461 ttaccgcaga aattgtaagc ttacgcaaaa agaattaggc gaaaaaatcg gtgtttccga
     2521 taaaacaatc tctagttggg aaaattctcg tacaatgcca gaccttgaaa tgctgtcact
     2581 tttacatcag actttaggtc tgccgattga ttttccttct attgaatcag ctaccgaatc
     2641 aatagaacca acattgccgg atgaacctct tgttatcaag aaatcttatt catcaaaaca
     2701 acccttctca ctcaaaaaga cactatcttt ctttctgctc gtcttttgtt tggtctatct
     2761 ttttcttatc aattatcaat ataaaaatga aacggttgat cgattcaatc cttttttgaa
     2821 accacaaatc ggctatacca cactccctgt aggatacgtg gaaaattcca tcgaacattg
     2881 ggtatgtaat gatatctttg ttcatggtac atggctgtct tttcacgctg gagaggtaga
     2941 aaaaggtaag aatcatgctg taatcaaaca caaaggaaaa cacgtcatga acgttcgcct
     3001 gctcacttgg gaagccattc cctcagatta ccaagaaatc ctcatccaag actatgacga
     3061 tgccttaaaa agaagccctc aggagccaga taggcattaa gtaggagatg aacgtagaga
     3121 tagaaaaaat caatgcacat gatcttatca aaaaaaaaca ggggtacaaa ttaattgaaa
     3181 aagctttgag atgaaagttc ctaaataaaa aagttcagaa tgataggtaa aaaaatgact
     3241 aactattcta gtattaggct atttgtatta ttcttctgtt atttttatat caaaaaatcc
     3301 agaaataact aaatataggt atttctggct tatagtataa tatttacgcc acagccccca
     3361 atgattcttt ttggaatcat gatcaaggac ggacatacac aaaaatattt tgcccattcg
     3421 atccgaatag attaacgact aatctagtaa gtaatataga ttggtacata gtcaaaccag
     3481 caaatggaaa agttgtctat tccaccccaa gggctttaaa gacagcatct tccggcgttt
     3541 gataaaaaat caaattaaag gtacccatta attctggggg tacagttcca atatcagcag
     3601 cgcttatcat aggtacaagt acttttttcg ctccggcatc ttttgctact tgtaaacagt
     3661 tagctaaatt atctacctta atcatagaac caccaattgt aatatcccct aaaataacta
     3721 aacttggtaa tacaggttta tttaaggaaa ctgagcataa agctattata gttgataacg
     3781 ataatgtttt tgtcatacca atgccattca tatcttgaac atgaacgaca aaatcttttg
     3841 tcgtcaaact aatagactga ctaacacttc cataattagc tcttaaatat cggtatgccg
     3901 aatccatcgc ttctcttgct ccagcatctg aattgatccc agtacgttca aactttccat
     3961 ttcccgaagt catttgcgtt tccaatttat aggtacctaa ttgcccagtt ttgccataag
     4021 ataccgtgta caaatgccct ggcttcccta acccttctgg aatcaaagtt cctccacctt
     4081 gttcaggaac agaaacatac tcttctcgct cttcttctaa atcaatgtat gaaaaattga
     4141 catcataaaa ttccattcca ccaattttct ttaattgttc ttttacacga cgacgcattt
     4201 caagagcaaa tcttagtatt tcttcgacat cacttttttc aaatctacca tcaggataca
     4261 acagtttcat catcccagat acaatccgac gaactgcaat cgtatcccgc tgatttaatt
     4321 gagaaccaag tttgaaatat ttatcaaaac tatcgccaaa actaactttt cgaagctcac
     4381 gcatgacttc agaaaaataa tctgtaatga agccatagtc atttgtaaaa aaatctggtc
     4441 gatatttagg aatttcccaa cctggtaaat aacaatgcat ccgatccaaa aaggctgaat
     4501 ccgtccccat aacctccgga aatggctcaa ataaattcga agttttcaat attacatcaa
     4561 cgctttgatt aatatttcct acaaaagcca ttgatgctga agcattcttt tcttctttgc
     4621 ctctagcaaa agatcctgaa gccatgtaat ctttcatgat ttgaacgcca tcattatcct
     4681 taaatttaat cccagcaact tcgtcaaaag caacacagtc ccacatacct acaagtccca
     4741 ttgtacgact agccatatta tagaaaaggt ttgctactgt tgtttgtcca cctgaaacaa
     4801 ggattgagtt tggtgaaatt tctttatata catgcgattt acctgtacca cgtggtccta
     4861 gttcacagag attaaaatta ttttcaatta atggaataag tcttgctaaa tgaagccatt
     4921 ttaccctttt attaaattgt gtaggttcca taccaatgga ccgtagaagt aagtcaatcc
     4981 attcatttgt agtaaactcc ttgcgagcca tcttgtaagc ctcaatgtct aaaccaggca
     5041 tttggattgg atcaaccttt tttgcaataa aagggctatt ccaacgatca ttctcatcat
     5101 accaatattc catcgaaaca atacaccaga tccccccacc taataatcga tcaaattttt
     5161 tcggatactc ttcactaatt tgaacatttt ttaatcccag gttagagaat tctgcttcat
     5221 aacaatcttt cttttcattt aaatgtacag ttactttatc aataactgta taaccaccca
     5281 attcacgaat ttttgctttt actttttctg cttcatcagg acgcacatag tttcgagcaa
     5341 gaatattttt aacattttcc acaccttttt caaccaatgt ttcatccaat gacgaagcat
     5401 acataccaag taaatattcc aatacataaa caggtacatt cgcaccttct ttaattttct
     5461 ttgttaaatc ttttctgaca atctttccgg gaaagaattc agataacttt aaaatttcct
     5521 gttgttccac tagaattcct ccatgtgttc agtcattata atagcaatta aagtatatca
     5581 tacttaacca gtaggcttga taatacaaaa gaaaaagtat ccacaaaaaa ttgtggatac
     5641 ctcgttttta tatatcaaaa tcaaatgatt ctgcaaaagg attatctata atgaatttta
     5701 ttcgctcggt caaatcattt ttttgatttt ttatcactaa ataataagta tcttcaactg
     5761 tatatcgacg atttacaaat tcaaaaacaa atttagtaaa ccgctgattt gcatcttctg
     5821 tttttgaatc cgcaacatag atttcttcat ttgagatacg ttcaccatat tcatcttcaa
     5881 aataaagcca gaaagatgcg gggtcataaa catcactaat tgcatttttt tgatagaatt
     5941 ctaacgtggt tgataatcct gtaattctgc ggttgctagt cattaattca attgctactg
     6001 gttcctttgc attgctacct ttggctaccg taatttctag aacagggact agcatttctt
     6061 gaatagatga ccctccatga acgtagcttt gtcccccgcc agctttctta aaaatacttg
     6121 aagtaatcgg gacacaaatg tttctagtgt catcatttcc taaagaatct cccaaagagt
     6181 aatttttcgt accaattatt tcgtattctt cttcactaag aataaaacga cgttctacac
     6241 gatctttttc atttctagat ggattttcaa tcttatctac ttcttcaacg tggcttcttt
     6301 tataaataaa tccatgatca gccgtaacta taaaacgata gatactgcca tcattatgca
     6361 agcgattaat aagtcgggaa atttcgctta tcgctaaatc acaggcctca aaaactcgat
     6421 tttcattcga gtgatcacct gttctatcca cttgatcgtg ataaatataa atgactgatt
     6481 tgccattaaa tacttctcgt acctcagcct tattcatctt aaccacatca tcataattta
     6541 aagctacagc atttgcatct cgttgctgga gaatcgcatt tcgattggat aaaccattcg
     6601 tttttttccc atcaatcagt acttctgttc cttcattgta agttagttga ttgtttggta
     6661 ataaggcggc tttaccgaat tcagtaactg aaggtaatac agaaagaaag ctagccatgt
     6721 ttgttgaata cttcttttct ctaactaact tacgttctaa attcatccct aactcatatc
     6781 gaaaagcatc tgaaataata actacagttt taacagagcg tttcattaca gcttgattat
     6841 aaaattttcg aatggatgga cgattcttaa attcaaaagt ttgattccaa atttgtcctg
     6901 attgatcaag aaacttcgaa taatttgctt caactaatcg atacaattgg ccatattttt
     6961 gcttttcttc tatatgatca ctattccaaa taaatttccg ataatgcgta tcaacccaat
     7021 acatttcctt ctcatattga gttagcaaat tatccatgtt catcggcaag cacaatctgt
     7081 ttttatccac aagataataa gcatgtttca ataattgata ctcataaata taatccgatg
     7141 cgaaatgcat tttttcgcgc tgctttaaac tatcaaaaat tgtttgtcct gcaattttcg
     7201 ttgtagtgtc agaagccttt aatcgttctg cataaaacat taatattttt gcatgaatct
     7261 cagaaaatac gtctgcttca attagatcat caatttctat actgtctaat aatccatcac
     7321 catcaatagc ctgatatact tttttagata gctgttcaaa gctttccatg taacgtgtat
     7381 cattcataaa ctgatccatg aaagtaataa tgtttgcttt caaatgtagt atttcaaatc
     7441 ctttgaactt cttcggcatc tcttgatcaa tttgataata aaaatagttc aaataaagac
     7501 acatcgtcag cttttccaat gttggctggc tatccgtaaa gccaaatttc atttgaataa
     7561 attcccaaaa tttagctgtt aactgatact tatcaagttc ccttaaagga ttctccctac
     7621 tttctgtaaa gctttttaga actacacgca gtaaatcatt taatcgaata cttttcgatt
     7681 tcaacaaaac agccattatc attaattcaa tcgtttctga tttattaatt gtcatatgcg
     7741 tactaaattc accaaaactt ctcatgcgat cctttgcatt aaagaaatta cgatactgtt
     7801 taactgtctg atgaaatcta agtggaatag ccaattcatt catgataatc gctaagcggt
     7861 ctgcactaaa ttcctctgaa taattcaata cactcaacaa atgattatct tcatctttat
     7921 catcccaacg aggaaatggc gcatagatta aatagttagt ttcgggatct tcatattcca
     7981 ataaacgctt agttttaaat tgtgcgtcct tttctaatcg atagattttt gcattttgaa
     8041 tgaccaatga gtcaatttct tcaagaaaat cctgatttga atcaaaccaa aaaatgattt
     8101 tcctacatcc ataattagga aaacgttgat ttaatttttc ttcaatttgt tgcgtattta
     8161 tatctgtcat caatctcacc taatcgttct ctttctatac cccttatatt gacataattt
     8221 ggataatttc gcacgcaatt taagaataaa aaagaaagtt gttagaaaac aactttctta
     8281 gataactatt tttttggttc tttgacccct tttgcaaaaa tttggaattt ctcgccggtt
     8341 ttaggatcac gctgcaactt atcataattg acaattacac catcatctaa gtctaattca
     8401 attcgtttta atgctaagtg acccaacttc tcgtcatact cacgaacttc ttttaattgc
     8461 ttctgaatct ttacaatttc ttttgtcatt gcatttcttt cttttgggtt atcacttgtt
     8521 gaaatttgtt gctgacgcaa ttcgattcga ttgtcatacg ctttttgtag ttcgtgtagg
     8581 tattcagtac gtacttttcc taattgatct ggtgtatagc gatgcaagta cattaacgct
     8641 ttaaagccat tttgttttcc tgagtccaat tgccaataaa ttggtcgatt ggagtatgtt
     8701 tttaaatggt ctttgtaaaa atcttgtacg aagtaacgac gaataatttc tcgacttgtc
     8761 cctttgccac ctaaagtgtc agcgataaac tggagatttt cttcaagggt ttctttacca
     8821 aaagtaatct caacgaaatc aatgaaacga ttgacaatgt caatttggct atccttgaaa
     8881 tactcatcat cggtgatgag taaaacatta tcagtatcag gtttaaattt ttgatagcgg
     8941 gaaggatccc attcgccgcc agcgaagatg agaccatctt cgtctaggga gtaacgaccg
     9001 aagatacaac ctattgaata agaaataaaa tccttaattt ccctcgcctt atcaggtaac
     9061 cttgcagaga tatattcatc ttgaacctct tttgttaatt ctttttcaag agaataatga
     9121 ttaatgaata tgtcgttcag ttcctcttca ttcgatttta gagtattaaa ttgattaacc
     9181 aactgcctac ttagacagtt aaatgcctct gagagatctt ttttaaacat ataacttaac
     9241 aaaatgtttc ttctaaactc gtatgacgtt tcaagagcat tatagtcttt tctagctata
     9301 tcgatatttt catccactt
//
LOCUS       DAKBVG010000106         9293 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000106 DAKBVG010000000
VERSION     DAKBVG010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.106"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(170..1537)
                     /gene="nhaC"
                     /locus_tag="OUD09_001760"
     CDS             complement(170..1537)
                     /gene="nhaC"
                     /locus_tag="OUD09_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HCT9693026.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(1646..3067)
                     /locus_tag="OUD09_001761"
     CDS             complement(1646..3067)
                     /locus_tag="OUD09_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCT9693027.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(3310..5187)
                     /gene="tdc"
                     /locus_tag="OUD09_001762"
     CDS             complement(3310..5187)
                     /gene="tdc"
                     /locus_tag="OUD09_001762"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HCT9693028.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(5478..6734)
                     /locus_tag="OUD09_001763"
     CDS             complement(5478..6734)
                     /locus_tag="OUD09_001763"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCT9693029.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(6846..7051)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            7314..8615
                     /locus_tag="OUD09_001764"
     CDS             7314..8615
                     /locus_tag="OUD09_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCT9693030.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(8684..9283)
                     /locus_tag="OUD09_001765"
     CDS             complement(8684..9283)
                     /locus_tag="OUD09_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCT9693031.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT         2807 a         1932 c         1657 g         2897 t
ORIGIN      
        1 agtaaagaac acttttgtct cacgctctaa acacgtataa acggcggaag cagaagcaac
       61 tccttcggaa ataagctgaa attcacaaaa atttaaaaag caattttctt gagtttcttc
      121 ttatttcttg gagttaaacg cttctgtccc agcctctttt ttccttcctt caatttttta
      181 cacgtcgacg attcaataaa aaaccgccta atattgtaaa gacaggtgca agaatcgaga
      241 aaaaggcgaa tggcaagtaa tgcaacgcgc taactttcat cgttcccata atgaatgtgc
      301 cgctgactcc ccacggtacg agagaattga cggtcgcacc tgaatccgct agtgttcgtg
      361 tcaaataggt ttttgataag ccaacgtcat caaaagaacg cttgaatgtt tctcctggaa
      421 gaatgatgga cagatactgt tctccaacga tcagattgat tccgatacag ctcaaggcag
      481 taaacccgac taaacgagaa ggtcggtcca tcttttcttt taattcttga acgatggtat
      541 caacgatcag atatttgatc agtaacccac ctaatgccaa tgcaagtaaa atcaaactag
      601 cagaacctaa catactcatg atcccgcctc gagaaagcag ttcatctagc tttggatcag
      661 gtgtatttgc gacataacca ttcatcaaga tgtctgctgc ttttccgacc gtcaaacttt
      721 tctcatggat aaaagaaaga acgagagcag ctgtcgatcc cgctagtaat gtagggatag
      781 ccggaatttt tttgattgcc cccccaaata aaataactag tggaatcaat gtccaaggag
      841 agatccaaaa gctcgctttc atgttttgga tcatttcatt gatactagct agatcggctg
      901 aatgactgtt atgtcctaat atcgtataaa aaatcaacga aaggacgaat gctgggaaat
      961 ccgtataacg cataccagcg atatgttcga atagattaac tcctccaata cccgctgcca
     1021 agttggtcgt atctgataaa ggcgagcagt tattccctaa aaaggcaccg gaaacgactg
     1081 cgcctgtagt cattgcatca ttgaatccaa gtaaatgacc gatccccaaa aaagcaattc
     1141 caatcgtcga aatcgttgta aatgaactgc cgacagtcac tccgacaatt gcacaaacaa
     1201 gaaatgctgt cggtaaaaag aaacgcacag aaacaagttt aaatccataa accataatcg
     1261 tagggatggt tccagataaa atccatgaag caactaagac accaatcaaa agaaaaatca
     1321 ggattggaat gatccctgga gtgattcctt tgatgatccc gtcatggatc tcatcccatg
     1381 tgaacttttt aaatctgcca taaaacaaca aacacatgaa agctgcaagg atcgggatat
     1441 gtggcgagag ctggaatccg atgatcagta ctcctaggag tgccaacaac acgatcaata
     1501 cagcgatact ttcttttaaa gaaatatttt tattcatcgt cgatctccta tttcaaatga
     1561 aaaggccctg ccatgaaaaa actattcact tggcaggacc tcctactaaa tcagcgacaa
     1621 aacgtcggct gaaagactta tttttttatt tttcttctaa acttttttct acctcaatcc
     1681 cccaagaatc tttcttgaag tgattgatga tcaatggaat aacgaacatg accactgtga
     1741 tagctgctac taataatgca tcactcaatg tcgctgtacc taatgtagca aatacgacaa
     1801 cgatgataga gaagatcagc atgcagctga ctacccaagc taatccgttt ccgtttttac
     1861 caattcggta aggacgttcg aattctggat gtttcttacg cattttaagc aatgaaacag
     1921 caatcaaaat gtacacgata caataaataa cagttgtagc atttgtaagt gtcaagaata
     1981 cagcacttac gtcatccatt acaccataaa gcaaagcaaa taatgaaata acgattgatt
     2041 gtgttagaac gacatttcta gatacgccgt atttgttttc tttatggaat ccgaattttg
     2101 gtggaaggaa tccttcacga gctacttgga tgattgtttt tgatggtcca gtaacccaag
     2161 ctgataattg taaaagcaca ccaataaata ccatgaagct gaagatattt ccgatcactt
     2221 ctggtaagcc taggatttga cagtcaatca agataggctg cgtgatattt gctaattcca
     2281 tttttccatc tggtacgaca tttgctacaa gcatcgcatt gatgacattc aataaaacta
     2341 atccgatcaa agcgataaat acgccttttg tataattttt tgttgcgtct ttcaaacgag
     2401 gcatgtaaac agaactcatt tcgatcccaa caaaaataaa tgtgatacca gctaagtatt
     2461 tcaatgaatc aatatgttcc aagtcaggga aagcttttga ccatgaaaag tcacctagat
     2521 agccgtttga gactagtccg actttgaagg ctgccaaaac acctaaaacg aacatgatga
     2581 ctactgggat atatacccct agccatacac cgatgttccc gccgactttt gccatatcga
     2641 atttcaagtt caagatcgtg atgatccagt agataactaa aatacaacct aaaacgaacc
     2701 agtgattatt gccaagttct acattaccaa atgtgtttcc taacaatggt cctaaagtag
     2761 aagcgaccat taccatacct gggaacattt gcacccataa tagccatgca acgacaaatc
     2821 cccatttgct gcccagtgct gtttttaccc ataactgagg accgccttct tgaggaagca
     2881 tcgtggaaag ctcaccggaa accattgata tcggaccggc aaaaaacaaa actgcaaaaa
     2941 ttgaataaaa aatcaatgtc caaccgactg cggctaacgt aggaatactc cgaaccgttg
     3001 cacacagtgc cattgtcaga ccgataaagg tcccaagacc cattttactt acttcagatt
     3061 tactcaccgt tgacactctc cttgattttt tggataaaat aacggtgtct ttgaagtagt
     3121 ttcagcaata aacgaaacaa ttcagcgaat atttgagggg attctttgag tttattgctt
     3181 gaagggtttc ttcaaagaca acctcaaaac aaagggatcg aagagcttag gtctcccctt
     3241 gctcttcaga tccaaacatg cgtcagaaac agttagtatt caagctgttt tctaacatga
     3301 ttaccttgct tattttgctt cgcttgccaa taattggtct gcgtagattt tttccaattt
     3361 ttcttgaaga gctgctttga ttttttcagc atattcttca aagtgttctt ctttgttcat
     3421 gtatggtgtc attacgctgg cacgcaatac agttactttt ccggcacggt tccattcttc
     3481 atctgagaat cctagttggt tcacaaattg taatgggctg ttgccatagt ctgggatagc
     3541 aaagtcagta tgtgaagtta agaactcatt accgtaaatg cttcctttca cgtatgaaga
     3601 ataatcatat acgtcatggt tcaatttatt cattgcaact aagtcatcgt tgcctttttc
     3661 tttgaataca tagtctacca tattgaaatc tggataagtt aatggatgaa cttcgatttc
     3721 tttgtctcct actttaaatg acaagtcatt caagaagttg tagaaacgat gtgcaccttc
     3781 gattgatgca cccattaatt taccatatcc tgtgacattc aaaggtaaga catggtgtgc
     3841 tgcccaaacg ctggctgctg ttgcccctgc ttttgaacct tccaagatat aagccccaag
     3901 taatgctggg atatctgcgc ctttttcaaa tacatatgtc gcaaagtaag agataacgtc
     3961 tctcatgcgg atatctttga tgacgatacc accggcagaa tatggaacat agcccatttt
     4021 atgtgggtca atcgttacag attctgcttc ttcgattgct ttatacgcac tgtgtacttc
     4081 ttctaagata taatctttgt tttctgtaaa gacattgtat ttgtaatgaa catcttttaa
     4141 atcttcaaat ggaatgaagt tgttgtcttc gtctaagaaa atcgcacggc cgtatccgcc
     4201 ataagcagca tctacgtgta agtagaagta aataccgtct ttttctaata cgcgacgcaa
     4261 tgcaacgatt ttatcgatac cgtcgattgc tccttcttct gttgatccta caacaccgac
     4321 tacacctagg attggtgttt tttcagctgc tagaccacga acgatttttt caagttcgtt
     4381 gatgtccatg cgatagttgt ggtcaactgg cactggaatc acttggtcta aaccgatacc
     4441 gatgatgtcg gcagctttta gccatgaata gtgtttagtt tgtggaacca accattttcc
     4501 taatttttct aaatgttttc cgctacgtgc tgagtgagct ttgatttcgt cgattttttc
     4561 tgggacgctg tctaacagat tcatgatttc ttctgttgac aagttcatta attcccaatc
     4621 gctttttcca gcaactagtt caggtgtcac ttctttcatt gcaagcggca atgatttgat
     4681 gttgcgggca taccaaagtc cttctaagtt tgctaaagaa ccatcagcaa cgatgtggcc
     4741 ccagccatct ttatagctca ttaattttgc aaattccatt cctacttctt cttccatttg
     4801 gcttgttgca ggagaagatt cataggcaac gttattacca ttccatagca tagcaaaatt
     4861 gtacgctagt aaagatggca tcaatgtttc agagttcata tgaccccagt aacgtccggc
     4921 attatgccaa ggaacggaat gggtacgcat ccgcgctgaa atttctgata gaacatcctt
     4981 tgttttattc acagtgtgtt caaaactagc actgcttttt tcttctggcg taataatagg
     5041 catgtcttga ggcatgtagt tttgacgcca gcctaaatgt tcatccacca attcattcaa
     5101 caaagcttta tagatttggc cgttctcagc tttgtcccca atgaataggg catttaggtt
     5161 tagatctttc gacaatgatt cactcatagt gaaaccctcc taaaaaaata tttaaatttt
     5221 gtcgagccct acattacacc tgttcttcta ttctgtaaac taagttttgg cctgattttt
     5281 aatcatattt taatatgtat caattatccg aattgttcac acttttgaaa taattaaaaa
     5341 aaacgaggga atttacaaat gtaaactccc tcgcaaagat tgtattaacg acaaccagcg
     5401 ttctatttta ggatgggttt caaaattgga atgttgtaat agtatttccc tttttcaagg
     5461 taaaaactta ctaaattcta atcaattact ttggccaaaa agtaattttt cttacctttt
     5521 cgaataacca caaacttacc atcgaattca tcggatggat ttatgacaaa atttaaatcg
     5581 gtaacgcggt caccgttaat acttatagct ccgtttgaaa catcttcacg tgcttgacgt
     5641 ttagatggtt caatttttgt ggaaacaagc agttccacga tattttcagg tgtgcttgaa
     5701 atttcaacgt tcggcatttt accgaatcct tgcgcgattt cttccgcatt tagttcttta
     5761 atatttccag agaacaatgc ttgcgttatt ttttgcgctt ctttcaagtc ttcttcacta
     5821 tgcacaaatc ttgtaacttc ttctgccaaa cggcgttgtg cttcacgttt ctcaggttct
     5881 gtctctactt ttttcgctaa gtcttcgatt tcttcttgag acaagaaagt aaagaatttc
     5941 aagtatttca ctacatcgcg atcatcttga ttcaaccaga attggtagaa ctcaaatggt
     6001 gaagttttct tcggatcaag ccaaacggca ccgcctgcag tttttccgaa tttcgttccg
     6061 tcagccttca gcatcaatgg gatcgttaaa ccgaacgctt ctgcttctgg tccttctaat
     6121 tttcggatta ggtctaatcc agctgtgata tttccccatt ggtctgctcc accgatttgc
     6181 aattgcacat cgtatgtttt atgcagatgc aaaaagtcga tagattgcag gatttggtaa
     6241 gtaaactctg taaatgagat tcctacttcc aaacggctgg caacgatatc ttttgctaac
     6301 atcgtgttga tgttgaagtt tttaccgtag tctctcaaaa attcaagtaa agagattttt
     6361 gacaaccagt cgtagttgtt cacaaatgtg atatttgcat ctttaccaaa caattttctc
     6421 atttgattgg aaagtgcgtc cacgttatgt tgcacttgtt ccatcgtttg caacacacgt
     6481 tctgttttgc ggccgcttgg gtcgccgatc gttcctgttc cgccgccaat cagaatatat
     6541 ggatgatggc ctgccaattg gaatcttttc atcatcataa atggaatcaa atgtccgata
     6601 tgcatcgaat caccagttgg atcaacgccg caataaagtg aaatgctctt ttcttcaacg
     6661 agctcgcgaa gtctttcttc gtttgtttgc tgattaatgg cgtcgcgcca agttaattca
     6721 tcaataatat tcattttact gacctccgtg tagttttttt atgaaagcag gaacctgcta
     6781 gcatgtgtaa ttaattattt cttcaggata ggataggaaa taaaaaaagt cccttggcat
     6841 ataagccaag ggacgaaatc tcgcggtacc acccaatttg tatgtctaga tatccaacat
     6901 acctctcgaa catttaacgc tgtcgcgtgg caatcactgc caaagactcc tgaaatgtaa
     6961 ttcgctttaa agcatgtact ggtttgcagc gaccaccagc tttctgaaac attggcttta
     7021 acaactactt cgtttcgatc ttcgtcttgt tattattaat atgtgtctat catagagcgc
     7081 aactttttct ttgtcaacag aaaaaggata ttttttttaa attcctcatt ttttcttttt
     7141 tgaatccgct ctaattccga acattttatt ttcaattatt gttaaaagaa ataacttttt
     7201 ctgtattttt tctgcaattt atcgagaagt cactttactt tgcctcttat tctttcttcg
     7261 ttacaataag gaatgggaca agtaaaaaaa tgaaaaagca agggacgagg tatatggaaa
     7321 aaaagcaaaa aattatttta ggagccactt ctgctttaac tatcgttatt ttaattctaa
     7381 gtttgttttt tgtcatccga tcatctacaa aaaaagaacc aatcattcct gaaaaaaaaa
     7441 taacagaaaa agcaaaagaa ccgacagatg ataagcaaca actagttact caagcaaaaa
     7501 atctggctca aagctacgat tatgaccaag cgattgcgtt attggaaaaa gatagtaaga
     7561 acggatctac tgaaacacaa cagcttcttc aaacactaaa aaaggagaaa gaacaactga
     7621 tcgactggtc tgatcctact cagatctctc atgtcttttt ccacagcttg atcgtggacc
     7681 cgcaaaaagc cttccactct caacaggcac aaggatacaa agattatatg gtgactgttg
     7741 aagaattcaa ccgttcgatc gaacaacttt atcaaaatga ttttgtttta gttaatttga
     7801 atgatttgat ccaaaaagac gaacaaggaa atctttcctt taacgggctt gcgctacctg
     7861 aaggaaaaaa accattggtt ctttcacaag atgatgtcag ctattatgaa tatatggata
     7921 attccggttt tcctagccga ttggtcgtat caaataacaa tgaaatcaaa aatgtctata
     7981 aagagaaaga taaagaagaa atcggtgact ttgacatggt tcctctgatc gatgcctttg
     8041 tcaagaaaca tcctgatttt tcttatcgag gcgcaaaagg taccttggcc ctgacaggtt
     8101 acaacggagt tttaggttat cgaacatcaa aatctcaata tggagacaat gaaaaaacaa
     8161 aaaaagagat taaagaagct aaaaaagtgg cggaacagtt gaagaaagac ggctggagct
     8221 ttgcttccca tacatggggt cacttgaata tgactcaagc ttcactagaa gatgtaaaaa
     8281 aagacaatga attgtggcag caagaagttg cacctatcct aggaaaaaca aatattctga
     8341 tttatccatt tggtgcagac atcagcgact ggcagcctta ttcagcagaa aatcaaaaat
     8401 ttgcttactt gaagagtcaa ggtttcgata ttttctgtaa tgtcgatgct tctacaccag
     8461 cttggggaca attaggtacg gatttctatc gaaatgctcg gatcaatatc gatggcattc
     8521 gttttcaatc tgatttagac ggaaaaaatc ctattcttga tccattcatc aatgtccatg
     8581 atgtctatga caaagaagca cgcgaatctg cttgatttta cgtcatttat gaaccaaaaa
     8641 agccgaactg acattttatc gtcagtttcg gcttttttat gctttatttc ttcttcggtg
     8701 cgattccctt gatcaggact tctgtccatt ctgctgtata tggaagcagt tttgcgtata
     8761 tctccggctt agaaattcct tctaccattg cttgaaaata gaggatcaag aactcatccg
     8821 aatatttcaa atccactttt ccttctttcc gccctcgatg gaaaagatca agcatgatac
     8881 tgaaactttc ttgcgtatac gcatccatcg cgtcagaaaa ttcttttttt tctttttttg
     8941 tatagtatgt catcaaatcg atataaaatt gtttgttcac tcgattgaga taacggattt
     9001 tgttttcact catggcaatc agtgtttctt caaatggaag attttccgca atcaacgctt
     9061 tcgcttcttg ccctaaatca ttcataaatg caaaaaatac ttcataaatc agcccttcct
     9121 tgctgccaaa atatttaaaa atcgtcgctt tacttacctg agcgttctca gctaccttat
     9181 ccattgtcac tccagcggtt cctatgtctg tgttcatcaa gtcaaaagct gcttgtaaaa
     9241 tctctttctt tttctcttgt gctctctttt caaaaccgtt catttgtaga gcc
//
LOCUS       DAKBVG010000107         8920 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000107 DAKBVG010000000
VERSION     DAKBVG010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8920
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.107"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="OUD09_001766"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="OUD09_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCT9693032.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="OUD09_001767"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="OUD09_001767"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCT9693033.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="OUD09_001768"
     CDS             complement(2169..2906)
                     /locus_tag="OUD09_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCT9693034.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="OUD09_001769"
     CDS             complement(2997..3524)
                     /locus_tag="OUD09_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693035.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="OUD09_001770"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="OUD09_001770"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCT9693036.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="OUD09_001771"
     CDS             complement(4401..4886)
                     /locus_tag="OUD09_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCT9693037.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="OUD09_001772"
     CDS             complement(5039..5236)
                     /locus_tag="OUD09_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693038.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="OUD09_001773"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="OUD09_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HCT9693039.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="OUD09_001774"
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="OUD09_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HCT9693040.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
BASE COUNT         2521 a         1982 c         1492 g         2925 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc
     8881 ctattaatag aacacttctc tcataaaaaa gtcattatga
//
LOCUS       DAKBVG010000108         8873 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000108 DAKBVG010000000
VERSION     DAKBVG010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.108"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            3..1457
                     /locus_tag="OUD09_001775"
     CDS             3..1457
                     /locus_tag="OUD09_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010825391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HCT9693041.1"
                     /translation="MTRFLRNYIDGRWVASRSTEFEEVMNPATEEALANVPLSSKAET
                     AEAVKAADAAFQTWKKVPVPVRGRYLFKLHQLLTDHKSELARLITLENGKSLSESLGE
                     VQRGIENVEHAAGITNLMMGDSLSTAATDIEITNYRYPIGVIGGITPFNFPMMVPFWI
                     FPMALAAGNTVVLKPSEKTPLLMEKIVELVEKAGFPSGVFNVVYGAHDVVNTLLQDPL
                     VKGISFVGSKNVGEYVYREGTKNLKRVQALTGAKNHVVVLKDANIDRAVKDLIGGAFG
                     SAGERCMAASVIVLEEEIADQFVEKFKAAAKAIKIGNGLDEGVFLGPVIRKDAQQRTF
                     DYIQKGVDEGAKLVLDGRENIPEKGYFVGPTIFDNVTMDMKIWQDEIFAPVASIVRVK
                     DLMAAVELTNESEFANGSCLYTNNASAIRYFRENVDAGMLGVNLGVPAPMAWFAFSGW
                     KHSFFGDLHANGKDSVEFYTHRKVVTARYAQSEI"
     gene            1533..2693
                     /locus_tag="OUD09_001776"
     CDS             1533..2693
                     /locus_tag="OUD09_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HCT9693042.1"
                     /translation="MNIQLKMPANLRIGGGTIQFLMEESEQLGNKPLIISDTVMEQTG
                     HLQKIKELLGKTDDQLAEYTDIQAEPTQENVYDALKLFQKHQCDHIIAFGGGSCLDTA
                     KVVAVLAANQLSLEAIHARNFDQLQAGIPLIAVSTTAGTGSEVTDVAVITNTKTAVKM
                     MMKDLVFMPQISIVDSDFTKTVPPKVTAATGVDALCHALESYVSVKANEVTQLFSLEA
                     MKKILNTLSQAYENSDDTEAREQMSLASTEAGIAFSNASVTLIHGMSRPVGALFHIPH
                     GISNAMLLPVFFEFSKDAIQTELATIARFIYPAAEDLSLEEAADYLIKKIQELCEQLS
                     IPTIRDYGIDQQAFMAALDKMAADALTSGSPQNNKKVPSHSQIKELYVQAYG"
     gene            2806..3813
                     /locus_tag="OUD09_001777"
     CDS             2806..3813
                     /locus_tag="OUD09_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCT9693043.1"
                     /translation="MRVKVGVIGTGAIGIEHIDRINQKTQGAYVTAVSDINVKAAKEV
                     AKRVDAKFFETGEALIASEDVDVVVVTSWDPTHEQYVLEAIKHGKYVFCEKPLAMDAA
                     GCRRIVDAEVAYGKKLVQVGFMRRYDRGYIELKEAIESKQYGEALMMHCAHRNPSADE
                     NYTTPMAISNTAIHEIDVLRWLLNEDYESVQMILPKKTSNSHADLHDPQLLIFQTKSG
                     VTIDLEIFVNCRFGYDIKCDVVFETAEVGMTDPAYTRIKSEEKNYTPVSPDWQTRFLD
                     AYDYEFKLWIDSIKNDAVVGPTAWDGYVAAITMMACHESRETKTKVMIQMDERPALYN
                     E"
     gene            3837..4862
                     /gene="iolG"
                     /locus_tag="OUD09_001778"
     CDS             3837..4862
                     /gene="iolG"
                     /locus_tag="OUD09_001778"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HCT9693044.1"
                     /translation="MNNIRVGIVGLGRLGKVHAQNLATGVSCCELTAACSLVETELAF
                     AQKKLGVKHTFSSYEEMVKSSVIDAVFIVSPSGFHCDQVRLAMENGKHVFTEKPLGLE
                     VEDIKKTQKVIEEHPEQVFMLGFMRRYDESYQYAKKMVEDGEIGEITLIRCYGIDPSE
                     GLESFVKFAMNNDSGGLFLDMAIHDIDLVRWFTGQEVDQVWAIGKNAAYPELDQAGEL
                     ETGAAMMSLSDKTIALLVAGRNAAHGYHVETEIMGTKGMLRVAQAPEKNLVTIMNDQG
                     IIRPCSQNFPERFREAFIREAQEFVSCIQEKRQPAVNAEDGLQSTKIALACQESFDTK
                     SLVQLKK"
     gene            4879..5769
                     /gene="iolE"
                     /locus_tag="OUD09_001779"
     CDS             4879..5769
                     /gene="iolE"
                     /locus_tag="OUD09_001779"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010777829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HCT9693045.1"
                     /translation="MTKQIQLAIAPIAWTNDDMPELGSENTFEQCISEMALAGFTGTE
                     IGNKYPKDPEVLEQYLNIRGLKVASAWFSAFLTTKPFEETAEAFKAHRDFLHAMGAKV
                     IVVAEQGHSIQGIMTAPVFDDKPVFTDEEWKKLAEGLEKLGDLAHEKDMEIVYHHHMG
                     TGVQTTEEIDRLMEMTDPDKVKLLFDTGHLVFSGENPIAVYNRHQDRIKHIHFKDIRQ
                     ETAEEVEKEKDSFLNGVKKGVFTVPGDGMIDFTPIWTAIHASDYEGWIVVEAEQDPAK
                     ANPFEYAVKARSYIREVTGL"
     gene            5792..7387
                     /locus_tag="OUD09_001780"
     CDS             5792..7387
                     /locus_tag="OUD09_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HCT9693046.1"
                     /translation="MNLFSIISFLVIVICVWLFAYSRSRSVDTSGSEGFFMGGRSLTA
                     LPIAGTIIMTNLSTEQIVGQNGQSYHAGMEVMAWEVTATIAIVALAVIFLPKYFKYGI
                     NTISDFIEIRYDTVTKRIISILFIVTYMTSFLPVVLYSGSLVFNKIFHIDELLGVQPI
                     VAIIIVAMAIGIVGILYLLIGGLSLSANSDSIYGVGLLACGLLVPILGLMKLGDGSFV
                     GGIEHVVVNTPWLLNSVGSIDSAVVPWPTLFTGMLFNNLYFWCTNQMIVQKALSGKNL
                     AEAQKGAFLVGVFKILGALFLVFPGIVARNIFGDSLMSNPDNAYPFLVSEVLPKMIFG
                     IFAAVIFGAILSSFAGALNATSTLFSLDFYKPLINKKANDKQIARAGKTVTVVVGIIS
                     IIVAPFISFAPAGLYQFVQEFNGLYNMPLLVIILFAFYSKKATAFGAKTTIAVHIVLY
                     ALSKMLFQNIHFLYVLTALFVLDIIILMVVSKLKPDGEFVLNSFDTKVDIEPWKYTKV
                     TAIALVVIVILTYVAFSPIGFAG"
     gene            7403..7717
                     /locus_tag="OUD09_001781"
     CDS             7403..7717
                     /locus_tag="OUD09_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HCT9693047.1"
                     /translation="MHKDLLIICETGISASLLVSKMLETIRAKNLSYEIDYATIRNVE
                     EKLNYKDYDVLLLTPQVARSEEKVKEIIKKEKCTAQIVYIKQEDFRYMNIENIIASIE
                     ED"
     gene            8178..>8873
                     /locus_tag="OUD09_001782"
     CDS             8178..>8873
                     /locus_tag="OUD09_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HCT9693048.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDT"
BASE COUNT         2705 a         1617 c         2090 g         2461 t
ORIGIN      
        1 aaatgacaag atttttaaga aattatattg atggtcgttg ggtcgctagt cgctcaacag
       61 agtttgaaga agtaatgaat ccggctacag aagaagcgct ggcgaatgta ccgttgtcta
      121 gcaaagcaga gacagccgaa gcagttaaag ctgccgatgc tgcgtttcaa acttggaaaa
      181 aagttcctgt tcctgttcgc ggacgttatc tgttcaagct gcatcagctt ttaacagatc
      241 ataaaagtga attggcgcgt ttgatcacgc tggaaaatgg gaaatcattg tccgagtcac
      301 tgggagaagt tcaacgagga atcgaaaatg tggagcacgc agcgggaatc accaatttaa
      361 tgatgggaga ttctttatca acagcggcga ctgatattga gatcacgaat tatcgttacc
      421 caatcggagt aatcggaggg atcacaccgt ttaattttcc aatgatggtg cccttctgga
      481 tcttcccgat ggctctggcg gcaggaaata cggtggtgct gaagccttca gaaaaaacac
      541 cattgttgat ggagaaaatt gttgagctgg ttgaaaaagc aggctttcct agcggtgttt
      601 ttaatgttgt ctatggtgcc cacgatgttg tcaacacatt gctgcaagat ccactggtaa
      661 aaggcatcag ctttgtagga tcaaaaaatg tcggtgaata cgtttaccgt gaaggaacaa
      721 aaaatctaaa acgggtacaa gcgttgactg gagcgaaaaa tcatgttgtt gtgctgaaag
      781 atgccaatat tgatcgggca gtcaaggacc ttattggcgg agcttttggt tctgctggtg
      841 aacgctgcat ggcagcctcc gtcattgttt tggaagagga aatcgcggat cagtttgttg
      901 aaaaatttaa ggctgcggca aaagcgatca agatcggcaa tggcttggac gaaggggtct
      961 ttttaggacc agtgattcga aaagatgcac agcagcggac ctttgactat atccaaaaag
     1021 gggtcgatga aggagcgaaa ctggtactag atggtcgcga aaacattcca gaaaaaggct
     1081 attttgttgg accaacgatt tttgataatg tcacgatgga tatgaagatc tggcaggatg
     1141 agatttttgc cccggtcgca tcaatcgtgc gggtaaaaga tttgatggca gccgttgagc
     1201 tgacgaatga atctgaattt gcgaatggtt cgtgtctgta taccaacaac gcatcagcga
     1261 ttcgctactt cagagaaaat gttgatgctg ggatgctggg ggtcaatctt ggggtgcctg
     1321 caccgatggc ttggtttgcc ttttcaggct ggaagcactc tttctttggt gatttgcatg
     1381 ccaatggcaa agacagtgtt gagttttata cccatcggaa agttgttaca gcacgttatg
     1441 cgcaaagtga gatttaaaga tttgcttcga agctctgtta aagagcttcg aggattctgt
     1501 tataggctat aagccagaga gaggtttttt ctgtgaatat tcaactgaaa atgcctgcca
     1561 atttaagaat tggcggcgga acaatccaat ttcttatgga agaatcagaa caactaggca
     1621 acaagccgtt aatcattagt gatacggtta tggaacagac cgggcatctt caaaagatca
     1681 aggagctgtt gggaaaaaca gatgatcaat tagcggagta tacagacatt caagccgaac
     1741 cgacgcaaga aaatgtttat gatgcgctga agctttttca gaagcatcaa tgtgatcata
     1801 tcattgcttt tggtggcggc agctgtctgg atacagccaa ggtggtagcc gttttggctg
     1861 ctaatcaatt aagtctcgaa gcgattcatg caagaaattt tgatcagctg caagcgggca
     1921 ttccgctgat tgcagtgtca acaactgctg gaactggttc ggaagtaacc gatgtggcag
     1981 ttattaccaa tacaaaaaca gcagtcaaaa tgatgatgaa ggatctcgtt ttcatgccac
     2041 agatctcaat cgttgactct gattttacga aaactgtacc accaaaagta accgcggcaa
     2101 cgggagtcga tgcgctatgt cacgctttgg aatcttatgt ttcagtcaaa gccaatgaag
     2161 taacgcaact attttcactg gaagcgatga agaaaatttt gaacacgctt tctcaggcat
     2221 atgaaaatag cgacgacact gaagccagag aacaaatgag cttagcttca acggaggcgg
     2281 ggattgcctt ttcgaatgcg tcagtcacat tgattcatgg aatgagtcga ccggtggggg
     2341 cactttttca cattcctcat gggatatcaa atgcgatgtt attgccggtc ttttttgaat
     2401 tctcaaagga tgctattcag actgagttag cgacgatcgc cagatttatt tatccggcag
     2461 ccgaagattt atcgctggaa gaggctgcgg attatctgat taaaaaaatc caagagctct
     2521 gtgagcagct gtcgatccca acgattcgag attatggtat tgatcagcaa gcgtttatgg
     2581 cagccttaga caaaatggcc gcggatgcgt tgaccagcgg aagtccgcaa aataataaaa
     2641 aggtgcctag tcatagtcaa attaaggaat tatacgtaca agcttatggg tagtaaaagg
     2701 cggagcaaag agagggctct accttttaag aataaaagct acacaggaat aattttgtaa
     2761 gcgcatacta tgtgtgctta aacaacagaa cagggggata agtaattgag agtaaaagta
     2821 ggcgttatcg gaacaggtgc aatcggcatc gagcatatcg acaggatcaa tcagaaaaca
     2881 caaggggctt atgtgacagc agtctcagat atcaatgtaa aagctgctaa agaggttgcc
     2941 aaacgtgtcg atgctaaatt ctttgaaacc ggagaagcat tgatcgcttc tgaagatgtg
     3001 gatgtcgttg ttgtgacttc ttgggacccg acccatgagc agtatgtgtt agaagcaatc
     3061 aagcatggga aatatgtttt ctgcgaaaag ccattagcga tggatgcagc aggctgccgc
     3121 cggatcgttg atgcggaagt tgcttatggg aagaaattgg ttcaggttgg cttcatgcgc
     3181 cgctatgacc gcggctacat tgaactgaaa gaagcaatcg aatcgaagca atacggagaa
     3241 gcattaatga tgcattgtgc ccatagaaat cctagcgcag atgaaaacta cacaacaccg
     3301 atggcgatca gcaacaccgc gattcatgag attgatgtgc ttcgctggtt gctaaatgaa
     3361 gattatgaat ctgttcaaat gatcttgccg aaaaaaacca gcaactccca tgccgatttg
     3421 catgatccgc agctgctgat ctttcaaaca aaatccggtg taacgattga tttggaaatc
     3481 tttgtgaatt gccgctttgg ctacgatatt aaatgtgatg tggttttcga aacggcagaa
     3541 gtaggaatga cggacccagc ctatacacga attaaatcgg aagaaaaaaa ttacacacct
     3601 gtttcaccag attggcagac ccgctttttg gatgcctatg attatgaatt caagctgtgg
     3661 atcgattcga tcaaaaatga tgcggttgtc ggtcctaccg cttgggatgg gtatgtagca
     3721 gcgatcacca tgatggcttg tcatgagtct cgtgaaacaa aaacaaaagt aatgattcaa
     3781 atggatgaac gaccagcact atacaatgag taattctcag aaaacggagg tatttcatga
     3841 acaacattag agtaggcatc gtcggtttag gaagattagg aaaagtacat gcacaaaatt
     3901 tagcaacagg tgtcagttgt tgtgagctta cggctgcgtg cagtttagtt gaaacagaat
     3961 tagcattcgc acagaagaaa ttaggcgtca agcatacttt ttccagctat gaagaaatgg
     4021 tcaaaagttc cgtgatcgat gcagtcttta ttgtttcacc atcaggcttt cactgtgacc
     4081 aagtccgctt ggcgatggaa aatggcaagc atgtctttac tgaaaaacca ttaggccttg
     4141 aagttgaaga tattaaaaag acccaaaaag tcatcgaaga gcatcctgaa caggtcttca
     4201 tgttaggctt catgcgccgc tatgatgagt cgtatcagta tgcgaagaaa atggtagaag
     4261 atggcgaaat cggcgaaatc acactgattc gttgttatgg gatcgatccg agcgaaggct
     4321 tggaaagctt tgtcaaattt gccatgaata acgacagcgg cggcttgttc ttagatatgg
     4381 cgatccatga tattgatttg gttcgctggt ttaccggcca agaagtcgat caagtgtggg
     4441 cgattgggaa aaatgcggcg tatccagaat tggatcaggc tggtgagctt gaaactggag
     4501 ccgcaatgat gagcctgagc gataaaacaa tagctttact agttgccggc agaaacgcag
     4561 cccatggcta tcatgtcgaa acagagatca tgggaaccaa aggaatgctg cgggtagcgc
     4621 aagcgcctga gaaaaatctt gtgacgatca tgaacgatca agggatcatc cgtccatgtt
     4681 cacaaaactt cccagaacga ttcagagaag ccttcattcg tgaagcccaa gaatttgtct
     4741 catgtattca agaaaaacgt caaccagcgg ttaatgcaga agatggattg caatcaacta
     4801 agatcgcgtt ggcttgtcaa gaatcattcg atacgaagag tttagtacaa ctaaaaaaat
     4861 aagcaggagt gtgagaatat gaccaaacaa attcaattag ccatcgcacc aatcgcgtgg
     4921 accaatgatg atatgcccga gcttggtagt gaaaatacct ttgaacaatg tatcagtgaa
     4981 atggcccttg ctgggttcac cggaacagag attggcaaca aatatccaaa agatccagag
     5041 gtgttggaac aatatttgaa tattcgcggc ttaaaggttg ccagtgcatg gttcagtgcg
     5101 tttctgacaa ctaaaccttt tgaagaaact gccgaagcct tcaaagcaca tcgggatttc
     5161 ttacatgcga tgggcgctaa agtcatcgta gtagctgagc agggtcatag tatccaaggg
     5221 attatgacag cgccagtctt tgatgataaa ccagttttca ctgatgaaga gtggaaaaaa
     5281 ctggcggaag gattagaaaa attaggggat ttggcccacg aaaaggatat ggagatcgtt
     5341 taccatcatc acatgggtac cggcgttcaa acgacggaag aaatcgatcg tttaatggaa
     5401 atgactgatc cagacaaagt taaattattg ttcgatacgg gacatttggt cttttctggt
     5461 gaaaatccaa tcgcggttta caaccgtcat caagatcgta tcaaacacat ccatttcaaa
     5521 gatattcgtc aagaaacggc tgaagaagta gaaaaggaaa aagacagctt cttaaacggc
     5581 gtgaagaaag gcgtcttcac ggttcctggt gatgggatga ttgatttcac tccaatctgg
     5641 acagcaatcc atgccagcga ctatgaaggc tggatcgtag ttgaagcaga acaggacccg
     5701 gctaaagcca atccatttga atatgcagtc aaagccagaa gctacattcg agaggtcacc
     5761 ggtctttaat ctattgaaaa ggagctagac gatgaatctt ttttctataa tcagtttcct
     5821 agtgattgtc atctgtgtat ggttatttgc ctattctaga tccagaagtg tcgatacttc
     5881 tggatcagaa gggttcttca tgggagggcg gagcttaact gctttgccaa tcgccggcac
     5941 gatcatcatg acgaacctgt ccaccgaaca aatagtcgga caaaatggtc aaagttatca
     6001 tgcagggatg gaagtaatgg cctgggaagt aactgctacg attgcgatcg tagctttggc
     6061 tgtgatattc ttgccaaaat attttaaata cggcatcaat acaatctcag atttcatcga
     6121 gattcgttat gatacggtta ccaaacgaat tatttcgatc ttatttattg tgacctatat
     6181 gacttctttc ttacccgtgg ttctttattc tggatcgctg gtgttcaata agatcttcca
     6241 tattgatgag ctgctgggcg tccagccgat cgtcgcgatc ataattgtag cgatggcaat
     6301 tggtattgtc ggcatcctct atctgctgat cggcggcttg tcgttaagtg ccaatagtga
     6361 ctccatttat ggagtcggtc tgctggcttg tggtctgctg gtcccaattt taggcttaat
     6421 gaagcttggt gacggcagct ttgttggcgg gatcgaacac gttgtggtaa acacgccatg
     6481 gctgcttaac tctgtgggat cgatcgactc ggctgttgtt ccatggccga cactatttac
     6541 agggatgctg tttaacaatc tgtatttctg gtgtaccaat caaatgatcg tccaaaaagc
     6601 attgtccggc aagaatttag ctgaggcaca aaagggcgct ttcctagtcg gcgtcttcaa
     6661 aatcttaggt gcgttattcc ttgtctttcc agggatcgtc gctcgcaata tttttggcga
     6721 tagtttaatg tcaaatccag ataacgcgta tcctttttta gtcagtgaag tattgcctaa
     6781 aatgatcttt ggtattttcg ccgctgttat ctttggcgcg atcttatcat catttgcggg
     6841 agctttgaac gcgacatcga ccttgttttc actggatttt tataaaccgc ttatcaataa
     6901 gaaagcaaat gataagcaaa tcgctcgtgc tggtaaaaca gtaacggtcg ttgtggggat
     6961 tatctccatt atcgtagcgc cattcatttc ttttgcaccg gctggattgt atcagttcgt
     7021 tcaagaattc aatggactat acaacatgcc gctgctcgtg atcattctct ttgcctttta
     7081 ttcaaaaaaa gcaactgctt ttggggcaaa aacgacgatt gccgtgcata tcgtcttgta
     7141 tgcactatca aaaatgcttt tccaaaatat ccatttcctt tatgttttga cggcattatt
     7201 cgttctggac attatcattt tgatggtggt ttccaaattg aaacctgatg gagaatttgt
     7261 gttgaactca ttcgatacaa aggtcgatat cgagccttgg aaatacacca aggtgactgc
     7321 tatagcattg gtagtcattg ttattttaac ttatgtggca ttttcaccaa ttggctttgc
     7381 aggctaaaaa caggagggta ttatgcataa ggatctattg attatttgcg agaccggaat
     7441 cagtgcgtcg ctgctcgtat caaaaatgct tgaaacgatc agagccaaaa atctatcgta
     7501 tgagatagat tatgcaacga tccgaaacgt tgaagaaaaa ctgaattata aagactatga
     7561 cgttttactt ttaacgccgc aggtcgcacg ttctgaggaa aaagtaaaag aaatcatcaa
     7621 aaaggaaaaa tgtaccgctc agatcgttta tatcaaacaa gaagattttc gttatatgaa
     7681 tattgaaaac atcattgcaa gcatcgaaga agattaacga gaatctttga ttattgagtg
     7741 tcgaggaggt ggtatatgaa aagatggcat gttcgccccg atcaccccaa tatgaagggc
     7801 ggacttatta gaaataccca atcttgtatt tattcaatag ggtaagcgga cctcaatagc
     7861 cagctcatcc ccaaaaagtc gaatggagac acttaaatga gtgtacctat tcgacttttt
     7921 gagactctaa gtcaaatcgt gttgttctca ttaaatcact gaaaggaaat gcttcggcgt
     7981 ttcctttttt ctttttgttt ttcttcgtgt tttgaaaatt acttactatt tactatacga
     8041 gtagtttcaa ataattttga catcctaatc ttcaacaaca gaaaaaaatc taaattataa
     8101 aatttagggt tgactaaaat ataatttttg tataccatat gatcgtgata atcattatca
     8161 tttagaagga gaaagttatg ttagaagtga aaaagcttaa tgtttgttat aacgattttt
     8221 tagcattgaa cgagatttct ttaaaaatac aagaaggtgc tttaacaggt attattggtc
     8281 caaatggtgc tggaaaatct acattgctta aagcaatgtt aaatattatc cctcatcagg
     8341 gagaggtctc cattaataat gaagatatca ataaaaaact ggtgaagatt gcatatgtag
     8401 agcaaaaagc tgatattgat tttacttttc caataaaagt aaaagaatgt gtatctatgg
     8461 gaacatatgc cggaatgaaa gtttttcaaa gaattaaaaa tgcagaatgg caaagagttt
     8521 caaaggcttt ggaaaaggtc gatatgggaa aatattctaa tcatcaaata ggagaattat
     8581 ctggaggaca atttcaacgt gtacttttag caagatgtct tgctcaaaat gcggatttca
     8641 tatttttaga tgaaccattt gtagggatag acttagttag tgaacgaata attatggata
     8701 cgttaaaaga attgaaacat cagggaaaaa cagtgctaat tgtgcatcat gatttaagca
     8761 aggtaaagaa gtattttgat aatattattc ttttgaatcg aaatctaatt gctcacggaa
     8821 gtgtagaatc tgtatttaat gaagagaatt taaaaaaagc ctatggagat act
//
LOCUS       DAKBVG010000109         8854 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000109 DAKBVG010000000
VERSION     DAKBVG010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8854
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.109"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(38..550)
                     /locus_tag="OUD09_001783"
     CDS             complement(38..550)
                     /locus_tag="OUD09_001783"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCT9693049.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(564..2861)
                     /gene="recJ"
                     /locus_tag="OUD09_001784"
     CDS             complement(564..2861)
                     /gene="recJ"
                     /locus_tag="OUD09_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCT9693050.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3003..3446)
                     /locus_tag="OUD09_001785"
     CDS             complement(3003..3446)
                     /locus_tag="OUD09_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HCT9693051.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3447..4235)
                     /locus_tag="OUD09_001786"
     CDS             complement(3447..4235)
                     /locus_tag="OUD09_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCT9693052.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4232..5173)
                     /locus_tag="OUD09_001787"
     CDS             complement(4232..5173)
                     /locus_tag="OUD09_001787"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HCT9693053.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5350..5937)
                     /locus_tag="OUD09_001788"
     CDS             complement(5350..5937)
                     /locus_tag="OUD09_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HCT9693054.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6158..7465)
                     /gene="obgE"
                     /locus_tag="OUD09_001789"
     CDS             complement(6158..7465)
                     /gene="obgE"
                     /locus_tag="OUD09_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HCT9693055.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            7768..8778
                     /gene="gap"
                     /locus_tag="OUD09_001790"
     CDS             7768..8778
                     /gene="gap"
                     /locus_tag="OUD09_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCT9693056.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
BASE COUNT         2441 a         2031 c         1541 g         2841 t
ORIGIN      
        1 tttaagaata acatcaagaa tagcttcttt tttctgctta atattccatt aatgtaatga
       61 tgtcatatcc tttgattttg tcgcgtccat ggagatccat caattcgatc aaaaatgcgc
      121 agcctacgac tacaccgcct aattgttcga tcagttcgat cgtcgctttg attgttcctc
      181 cagtagctag caaatcgtca caaatcagca cacgttgacc tggtttaatg gcatccttat
      241 gcaatgtcaa tgtatctgtt ccatattcta atccataagt cacttcgatg gtttctcgag
      301 gtaacttccc ttttttgcgg actggggcaa aaccgactcc taactcataa gcaacaggac
      361 atcctacgat gaatccacgg gcttctggcc caactaccat atcaattcgt ttttcttttg
      421 cataatttac gatttgttta gtagcttccc gataagcgtc accatctgcc atcagtgggg
      481 aaatatctcg aaacacaatt ccttttgacg gatagtcggg aatactggca atataatctt
      541 ttaaatccac tctttgtttc ctcctaggtt gttaaccatt tctttatggt aggaatatcg
      601 ctcaaaagca aaaattcctc aactttgatt ttattgagcc gtcgctggta taatctgctc
      661 tcagtcaaag gatgactttc tgggctagcg actttttgta aaacaccgtt ctctattgta
      721 acaaatttca attcaaaaaa cacctgtatc ataaaaatta ataatttttc agggatacgc
      781 aaatattgag ctattgcttt tattttatga cgtatatcaa ttcgttcttg agaatggatc
      841 aatttaaaca attttgcgta ctgttctctt gtgcctaccc cattcaaata ggcctcatca
      901 ggtgaaatcc ccatcagata aacacgattg aatgaataag accgaaaaac ttctttcaga
      961 tccgctaaat catctggaca atccataaca actagagagt ttggttttct tttttcaatt
     1021 attgccttta cagaatctag tccttcgaaa acaatcgcag cttcctggat aatctcaggc
     1081 acgaatttca acgtcgaaga atcaaatgct aaataaaggc taccttccga cgttcggctc
     1141 tgttcacgga aacgttttgc ccgccaatca aatacttgca gaccacccac agaaaaatca
     1201 gataccatca gctgcggttt cttacgcccg ttccattcat tgatcgaaag ctttcctacc
     1261 acatctacct gattattcag cagttcttct tcttgagtac caaaaccaaa agcgaccgca
     1321 tcaagctgac tcgatgcatc ggaaaggaca aatttcagat gctgttggtt tgctccgatt
     1381 cttttgatat tttctgcttg gacttgtcga aataaaaact gaggcaaagg attatccgta
     1441 ccaaatggcg ccaatagttt cagctcatca attcgctcta ctgttacttc atttggcagc
     1501 aatacttcat caattctcaa ctcaggccct ctcatcaagt caatttgatg atcgactata
     1561 tattggttca tcttctcctg tagtatagtg acattttcac ttggcatcgt taaaccgact
     1621 gctgcatgat gtcctccgaa gtaagtaaat aaatctcgca tctggtcaag catttcaaaa
     1681 agattcaagg cttcaatact acgtccagat cctttagctg atccatcttc ttttaaagta
     1741 aggacaagcg ttggtttacc cgtttcattc attattttcc cagcaacaat gcccaaaacg
     1801 ccttcatgcc atcctcggtt taccaataga tggatatgat tctccttatt gaccatcgct
     1861 aatgcttctt gtgttatctg ctctacgatt gcttttcttt cttcattgat ctcattcaat
     1921 ttttttgctt gaacggatgc ttcctcttca tcaaacgtcg caagtaacga gacggcagga
     1981 cgtggatcgc ccatccgacc gattgcattt agtctgggag caatcgaaaa accgatcgtt
     2041 gtttcgtctg cgtccctcat ttttacgccg ctttcttcaa atagcgcctg aagtcctatc
     2101 cgttctgaat gatgaatggc atcgattcct aatgaaacaa ggattcgatt ctcatcagtc
     2161 aatgaaacta aatcggcgat cgttcctatc gctactaaat ccaaaaattc tgcaggcggt
     2221 tcttccaata atgcagaagc tactttgaat gctactccga ctcctgccaa atctggaaat
     2281 ggatagtgcc ccgctggatg acgagggtga acaatcgcat aagcgtctgg taaattctca
     2341 ggtagttcgt gatgatctgt aatgatcacg tctacaccag cttgctgagc gtatgctacc
     2401 gcatcatttc ctgctacccc gttatcaaca gtgatgatca actggattcc ttcttcgatc
     2461 ttttcttggt ataccgattg gtttggtcca tacccatgct caaatcgatt tggcaggaat
     2521 acttctactt ctgcaccaag taattccagt gtttctttca ttaccgttgc actggtgatc
     2581 ccatctgcgt cataatcacc gtaaacgagg attttttctc cactgattac agcttcttgg
     2641 attcggctga ttgctttatc catatcgtgt atcagatacg gatcatgtaa ttcttgttcc
     2701 tttggatgta aaaatcggtt gatatccttt tcctcatggt aacctctttg ccacagtaac
     2761 ttaccgacaa atggagataa tttcagttca cggatcattt ggagaaagga ttcagaaggc
     2821 tctgtctcgc ttaacttcca atcaaaattt gcttgtctca ctttctcact cctaacttag
     2881 ttattatagc aaaggaaatc agcaaaaaaa agcagaaaga aagaataaaa acagatcaga
     2941 tccgctagac agatgttcag caggacctga tcttttgtac gaccatttat gaaatatccc
     3001 tattagtcaa aataattgaa gctttgtttg ggcgattgag ttttttgttc ttccggcgct
     3061 ttcaattgac tgatttgtgc ggttttcgct tgtaactccg cctgataagc agcttccaac
     3121 tccgcacgtt cgtttctaaa ctcgcgttgc tgttcttctt ttgcttcttc aattttcttt
     3181 tgaagttctg tttgatattc tgacatttcc tgagtcagct gtttgatttg ctttttttgc
     3241 tgccatagtg ttgccgaagc agtcaaaaat acaatcagtg caccaatcag cgcagaaccg
     3301 atgatcacta agatcaatgg cgcagaaaag gaagtgaagc caaaacttac aggaacattt
     3361 ttattgttca atacagcaaa aagaacaata atcaaaacta aaacaaatcc aagaatcaca
     3421 ttgctttgct ttttcaattc cttcacctac tttttgttaa aaattccgct ggctaaataa
     3481 tcaccaatcg ttggaaacag cgtataaaat tttgatgcaa cttctaaaat aaacggttga
     3541 ttgatttccc gttttggatg tctcatattg cggacgatca tttttgccaa cttggcaggt
     3601 tctaaaacga tttgtccgac tttttccaaa tagcttccgc ttggatcagc cttatcgaag
     3661 aaatttgtct cgattggacc tggattcact gtcgtaacag ctacgccaag tggtttcaat
     3721 tctaatcgca aagcgttgga gaagccaagg actgcaaatt tcgtggcaga ataaaccgtt
     3781 gattttgcag ttgccatttt tccagccata gaggcgatat taatgatatg accttgtctt
     3841 ctttctgcca tatcgatcgc aaatttttgt gtcaaaacca tcattcctaa gatgttgact
     3901 tcaaacatat cgtatgcttt tccaaggtcg aatgtaagga agtcttcgaa aatcccaaaa
     3961 cccgcattgt ttaccagaat atcgacgcgc ccgacttctt ccatcacctt ttcatataat
     4021 tcgtccacgc tttcaggatt gctgacgtct accttgaaag cataagcctc tttgccgctc
     4081 aagcgtctgc actcgtctct gacaccttct acaaatgcca ttcttcgtgc gcaacttata
     4141 acgattgctc cttgctttgc tgcttcataa cagatttgtg cgcccagacc gcctgaacta
     4201 cctgttacta aaacaacttt atcttctaaa ttcatgcctc ttcctcctcg cgaaatggaa
     4261 tctcaatgct atcaaaatct ttcataatcg tcgtgttttc aaacacttct tgtgcttctt
     4321 tttcaagctc taaagctgct tttcctaaat atctcgcact gatatgagta agtagcagcc
     4381 gttttgcctg cgcttctttt gcaacttcag cagcttgatg agtcgtggaa tggaaatagt
     4441 tgtgcgccat tcttgcttca tctttattaa atgtgctttc atgtaccaga atatcgctat
     4501 tttccgcaag cacaacggaa ttatgtgttt ttctcgtatc gcctaaaata gtcacgattc
     4561 gtcctttttt atctggacct acgaaatctt ttccattgat cgtccgccca tcttctagtt
     4621 ggatcgtttc accttgcttc agtttgccgt acagtggtcc agctggaata cctaattctt
     4681 ttagacgatc cacctgcaat tctcctttat gatcatgttc aaccacacga taaccaaaac
     4741 tgtcgatccc atgatttagt ttttttgtat aaacagaaaa ttgctgatca gtaaaaatcg
     4801 gggctgtttc atttagctca atgaatttca gcggataact taaacgcgtt tgcgaaatac
     4861 ctaaagagac ttttatgtat tcctctatcc cttttggccc gtaaatctct aatggcgtat
     4921 ccccaccttg aaatgaccgg ctgctgatga gcccgggaag tccaaaaata tgatcgccgt
     4981 gtaaatgtgt aatgaaaatt ttaccgattt ttctcggacg aatagtcgtt cgcaaaatct
     5041 gcatttgcgt gccttcgcca cagtcgaaca accaaacttc attgcgctcg tctaaaagtt
     5101 tcaatgctat acttgtcaca tttctatgtt ttgcaggaac acctgcacct gttcctaaaa
     5161 attgtaattc cataatctac ctgactttct ttaagttcct ctttaatttt agggtaaaaa
     5221 gaagggataa tcaaccgtaa gtcgagaatg ggttcaaatt aaaaataacg ttctaaagcg
     5281 atagcgtttt ctttgaaaat tactataaaa acagcagcga acccgtttga gatcctctgc
     5341 tgttttttat taatttgaag atccagaact ttcattgtcg ttggaagttt gcggatgttt
     5401 gtccttccaa gtcttttgtg cagcttcttt cgcatgattt tcagcttctt tcgcatcatg
     5461 cacatctttt tcgacatctt cataatccaa gcgagtaatt tctccgccta attccatcat
     5521 taccaattga ttcagtgggc gattgtcctc ttcatcagca atcaagatca gtcctgtagc
     5581 tccttcaccg attttatttc ctacaaagtc gaacacggtc tgggcatctt ttatttcttt
     5641 ggcatcttgt gtcgcaccga tcattcctcc ggcaaaccat cctaataaaa tccctagcgg
     5701 accacctaat acaccaacca acattccgat taatccaccc gtagaagatt tgttattacc
     5761 ggtaaagtcg ataaaatctt ctattttgaa ttgatgtgtc ccattaggat catggctgac
     5821 aactgccatc tgttctcctt taatcgaacg aacagcatgt aactttttga tttctgaaaa
     5881 tgcctgatat gcctggcttt ctacttgaaa atttaaaatg attactcgtt tgctcattaa
     5941 aaacgcccct ttcatacctt cttgttattt attttacctt taattgtaaa ataagaaaag
     6001 aaaagcactc tttttttcca ataaaatagg gctgtgacaa aagtcgtatc acagctcctt
     6061 tttccgaata aacggcgttc ttgaagctga tcacttctgt cacaacctcc cttaaatctg
     6121 aatctatatt ttttggtatt tgtttttatt tgcttgccta ttcgacaaat tcaaattcaa
     6181 actcaccgat acgtacaagg tcgccatctt tcgcaccgcg tgcccgaaga gcttcatcca
     6241 cacccatacc acgtaattga cgagcaaaac gcatcactgt ttcatcatga tcgaaattcg
     6301 tcatttggaa taatttttcg atcttctcac cagataagat ccacgttgca tctgaatcac
     6361 gatcgatttg gaactcagga ccttctggat tgaatccata gtgtacagtt tcttcttcca
     6421 gttcttcttc gtacaatggg aattctggcg ttacttctaa caaatctgct gtagcattca
     6481 ataacgcgtc caatccttgt cgagtcactc cagaaattgg aaatactggg atgtcatcag
     6541 caaattcatc ttctttttcc ttgttcagct gttctttgaa tttcactaga ttttccggtg
     6601 catccggcat atccattttg tttgccacga tgatttgcgg acgctctagc aagcgtaagt
     6661 tataagtaga taattctttg ttgatggcaa gatagtcttc ataaggatct cgtccttcca
     6721 tcccactcat atcgatcaca tgtaaaatta ctcgtgttct ttcgatatga cgaaggaatt
     6781 gtgtacccag cccaacacct tgcgaagctc cttcaatcaa gcctggcaag tctgctgcgg
     6841 caaaacttct gccatcactt gtagtaacca ttcctagatt aggtacgagc gtcgtgaaat
     6901 gataagcgcc gatttttgga cgagcagatg aaataacgga tagtaaagtc gatttcccga
     6961 cagatggaaa acctacaagt ccaacgtctg ccaatacttt caattctagt tcgatttttc
     7021 gctcttgtcc tggttcaccg ttttcagcaa tttccggtgc aggattccga ggagaggcaa
     7081 aacggatatt ccctcgtcca ccgcgtccac cttttgccac aactagtgtt tgaccatttt
     7141 caattaaatc tcctaaaaga gcaccagtct ccgcatctcg gacagtggtt ccttgtggta
     7201 ctttaacata ggtatcttct gaaccacgac cgtgcatccc tttactcatc ccattttcac
     7261 ctggctgcgc tttgaaatgg cgattgaacc gaaaatccat caatgtacgt aacccttcat
     7321 cgacgatcaa gatgacatcg ccgccacgac caccgtctcc gccagccggt ccgccgtcag
     7381 gaacgtattt ttcgcgacga aatgcaacca ttccgtctcc gcccttgcca gctttcacat
     7441 cgatcgttac ttgatctaaa aacatggaca taattaagtc ctcctattat tcatttattt
     7501 atttgactgc ctgaatttcc acaataaaca aaagtcttac ttattttatc ggtgaaatct
     7561 gattttgtct atcatttcag caaaaagatt gtttaccttc ttcttttttt atcatttggt
     7621 aaggtttcca accttttttc tgctaccaag tataattttt tgacaaaata ttctttgcgt
     7681 agcagaataa cagtgtatta attgtattaa ttgtattaat tgaggatgcc gtttcacagc
     7741 acccctaata aaggaggaac ttttgatatg acagtaaaag taggtattaa cggatttggg
     7801 cgtattggcc gtctggcttt ccgccgcatc aaagaagtct cagatgatat tgaagtagta
     7861 gcaatcaacg acttaacaag tccaactatg ttagcacaat tattgcaatt tgactctact
     7921 catggtactt atcctggcac agttactgca acagaagact caatcgtagt ggatggcgaa
     7981 gcaactcgtg tttatgcaga acccgacgca agcaaaatcc cttgggcaaa agaaaacggt
     8041 gtagatatcg tactagaatg tactggtttt tatacatctg aagaaaaagc aaaagcgcat
     8101 ttagatgctg gtgtcaaacg agtagttatt tctgccccag ctggtgctat gaaaaccatc
     8161 gtctataacg tcaatgatga tacattagat gaaaacgaca aaatcatctc agcaggttca
     8221 tgtacaacaa actgtttagc acctatggct tatttcttaa ataatgaatt tgggatcgaa
     8281 gttggtacaa tgacaacagt tcatgcctac acttccacac aaatgctctt agatggacct
     8341 gtaaaaggcg gtaatttgcg tgctgctcgt tcagctgctg acaatactat cccacattct
     8401 actggtgctg cgaaagcaat cggcttagtt atccctgagc tacaaggaaa attacaagga
     8461 cacgcacaac gtgttccagt cgttgatggt tcattaactg aattggtttc agtattgaaa
     8521 acaaaagtaa ctgctgatca agtaaacgaa gcaatcaaaa aacacacagt tgacaatcct
     8581 tcattcgggt atgatgaccg tcaaatcgtt tctagcgatg tcattggaac aactcaaggt
     8641 tcgatctttg acccaacgca aacagaagta acaactgctg gtgatttcca acttgttaaa
     8701 actgttgcat ggtatgacaa tgagtatgga tttacttgcc aaatgattcg cttactagaa
     8761 aaattcgcta acttgtaatt tgatagatca atcgataaaa gaagcctttg actggatcca
     8821 gtcaaaggct tctttctttt catcccctac tttt
//
LOCUS       DAKBVG010000110         8782 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000110 DAKBVG010000000
VERSION     DAKBVG010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8782
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.110"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            68..1189
                     /locus_tag="OUD09_001791"
     CDS             68..1189
                     /locus_tag="OUD09_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HCT9693057.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            1196..4324
                     /locus_tag="OUD09_001792"
     CDS             1196..4324
                     /locus_tag="OUD09_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HCT9693058.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            4343..7537
                     /locus_tag="OUD09_001793"
     CDS             4343..7537
                     /locus_tag="OUD09_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HCT9693059.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(7683..8390)
                     /locus_tag="OUD09_001794"
     CDS             complement(7683..8390)
                     /locus_tag="OUD09_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCT9693060.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT         3159 a         1519 c         1811 g         2293 t
ORIGIN      
        1 aagacaggtg tcatgtaatg gatcaaagtg ttataataat tagaaggaca ttttttgagg
       61 tgattgaatg cgttttttac atacagccga ttggcatatc ggaaaaaaac tacaaggtta
      121 tgacttgatt gaagaccagc gtcatgtact atcttctata ctagatatcg cgaaagaaga
      181 aaaagtagag gcaatcgtga ttgccggaga cttatatgat aggagtgtcc cagctgttga
      241 tgccgtagaa ttatacaatg aattgatggt cgattggaat ttaaaggaaa agatcccgat
      301 ttttgctata tcaggaaatc acgatagcag tacgagactt tcagcgggta cgccatggtt
      361 cagttattcg aactattttt tgcgaacaca gctagcagaa gcatttgaac cagtcgagtt
      421 taaaaacgta caatttttct ttcttcctta ctttgaacca attgcagcga gattatattt
      481 tgaagatgac aatatccgaa cgatcaagca agcggtagcc aaggtgatag ataaaatgca
      541 ggagctattt gattcagaaa aaaaacaagt attgattagc catttttttg taatgggaag
      601 tgtaaaatct gattcggaaa cgaaaatcga agtaggcggg ttggattcga tccctggtga
      661 actacttaca ccgtttgatt atgtagcact aggacatttg cataatcaag ctgctttgag
      721 acaggataat gctcgctata gcggttcccc attgaaattc tctttatctg agatcaacca
      781 aaaaaaaggc gtatggatcg ttgacctgac aacaggcctg gagttgaatt ttagggagat
      841 cgaacccctg catgatattg aagaaataac agcgagtttc aaagaattgc tggatcctgc
      901 cttttatcag tccgtcaatc gggagaatta catctatatc tatctcactg atagaacgat
      961 catcccgaat atgatgaacc agcttcggca aatatatcct cgtattttgg ggattgaacg
     1021 ggcaaacggt cgagcagaac aaacaaagcg caaacgaaaa gtactagaaa tgaaagatcc
     1081 tcaaaaatta gcagaagcat tttttagtga aatgacggaa gagcagctaa ctgcaaacca
     1141 agaaaaatgg ctcggagaaa cgttgcaaga aatcatggaa atggaatgat caagaatgaa
     1201 accgaaaaaa ttacaactta aaaattttgg tccttttatc aatgagaccg tagatttttc
     1261 acgtttgaca gaagcaccgc tttttttgat cagcgggaag acaggagctg gcaaaacgac
     1321 gatttttgat gggatcacct tcgcactttt tggtgaaact tccggaagac ttcgctctgg
     1381 aaaagagatg cgttctttgt ttgctacacc agacgaagaa acaagcgtga ctttttcttt
     1441 tgaacatcag cagatgagct atgaaatcga acgaaaacca gaacaagttt tagcaaaacg
     1501 aaaaggaaac ggaacaaaaa aacaggcagc caaagtaacg ttgaccattt ttgatgggaa
     1561 aggaaaagaa ataaaacaaa tcacaaaacg tacagaagca gatcagttga tcagagaact
     1621 gatgaatctc gatgcgaaac agttttctca aatcgtttta ctaccccaag gagaattccg
     1681 taattttttg gtttcatcaa gcagtgataa agagactgtt ttacgtcatt tattcggtac
     1741 tcaatttttc caacagttca atgagcgatt aaaagaaaaa gcaaaacaac agcaacaaaa
     1801 attggatcat ttagagcaag agctgcatct tctgcaaaaa aggtttgttt tagtcaaaga
     1861 agaggaacca gcagcagggt ttgaagccgt tttagcccaa tggacaaacc atcaggcaat
     1921 actcgaaaaa caaatcacgg aagaaaaaga agctctaacg gaacttcagg aaaaacaaaa
     1981 gcaactagaa aattcctact atcgttttga aagcttgaaa aaaagttatg aagaaaaaac
     2041 gatattaata aacaagcaaa aggaattagc tgctcaaaaa gacgaaatag aggaaaagaa
     2101 acgatggatt gggtattatg aatttacgca acgattagtc gaaccaatcc agcgcctaca
     2161 agaaataaaa gttgaaaaaa tagaactgtc acaaaaaagt agtgagcaaa caaagaaact
     2221 agcgatgact acgcagaatt atgaagagtg gcaaaaaaat gaagcaaacc gcgtacgccg
     2281 acaaacaaaa atcaaagaag ctgctcaaaa actgcaaaac aaacagatga tggtgccaat
     2341 tgtcgaatcc ttacgtagta aaaaacaaga cgcagaacaa ttaacaattt cattggataa
     2401 agaagaattg aatgccgaga agctgtatca aaatctcgct atctatcagg aaagaactcg
     2461 aattcttcaa gaaaatttac tggatcagga aaaactgcaa gaagatcgtc ttgcttttgg
     2521 acagttaaaa tatctagatg aaaagcagca agaaatagaa aaacaaaaag caaaagacac
     2581 tgggtcgctc gcacagataa gagaagaact caaaaaagca actgaagaaa taacaaaaaa
     2641 tagtcagcta ttgaaagaac agacagaaca agcagaacag ctgaaaagcc aatgggcaaa
     2701 gcagcaaatt gctcgtcttc agcttttttt gcgtccaggg gagccatgtc ttgtttgtgg
     2761 atcaacggaa caccccatac aacaattaaa acacgaacag ccaaataagg aagatatctt
     2821 aaaagtagaa caacaactgg cagaagctga gtctgctgta gaaagaactc agcaaatcat
     2881 tgccaaaagt gaagcacatg aaaataatct tcaacaacaa ttatcagatt tagaacaaaa
     2941 aatcatggca acgaatatac aattggctga acaagaagaa cacttgcaac aggctttgaa
     3001 aaaggcattt cctgagataa cagaacaaaa aacaaaaaca actcttgtag taatcgaaca
     3061 atctttagat agacgaaaaa gcaagatggc caatgcaaga atagagctgg aagaaataag
     3121 acaacaagca gaaaagctcg aacaacaatt agctgtctac aaacagcagc agacagaaaa
     3181 aaataatcag ctccagcaac tgcaaggaga aatcaaaagt ttagaacaac aattgcaaga
     3241 tacggatagt aactgtttag aagaggagat acgacgactt gaaaaacagt tagaagaaga
     3301 ctcgaatcaa ctagctgttg agcagaaagc aggagtgcaa ttgttgcaag atcttcatac
     3361 gcttcggaca cagcttgatc ttcaacatga acaaatagaa aaaatagatg aaaaaataga
     3421 aaaggaacaa gaaagaatag ctaaaaagtt agaaagccag tcgtattttt caacaattga
     3481 agaaatctgt gcatttgcac aacagcaagc acaatataaa gaaaaccaac gtacagttac
     3541 ttcttatcat gaagagcaat tagtgacagc tgaccggctg cgtcaattag aaaatatcca
     3601 gctaactgag acattcccag atacagaaga gatcagccaa cagttagaag aaataagacg
     3661 tatgatcgaa ataaagcaag aaaagatata cggtttacaa gaacaaaaaa ttagtaatca
     3721 aaaaatctac gatgaatgtc tttcgattta tcacacaagc cagacgaaat tagatgagct
     3781 tacgcaatta cagcagcttt cgcaaacatt caacggagaa aatcctaaga agactagttt
     3841 ggaacggtat gtgcttcagg tttatcttca agaagtgtta caagtagcaa atgatcatct
     3901 ccaacgttta acgaaaaatc gttatcaatt tgagctagcg gataccattg gcagttatcg
     3961 agggaaaacc ggattagaaa tcaatatcta tgatgatcag gcaggtatgt ctcgaagtgc
     4021 gcataccctg tctggcggtg agagttttat tgcagcactt tctttagcat tagctcttgc
     4081 cgatgtgatc cagacacaag ctggtggtgt agcaatcgat gcattgttta ttgatgaagg
     4141 atttgggtca ttagatgaag aagctttgga aatggcaatg gaagctttag aaacgatcga
     4201 aagtgaagga agaatgatcg gcatcattag tcatgtaaga gaattgaaag aacgagtatt
     4261 acaacaaata agaattgata cagaaggtag cggacaaagc aaaatacgct atcaacttgg
     4321 ataaaaaggg agattggtaa ctatgaaatg gagtattcca gaaaaagtca ttgcacgtgg
     4381 acgtcaatac ttgcaagaag gccgagtgtt atcggttgtc cctgaccaag aagaaaacgt
     4441 gtggcatgca gaagtcttgg gcagtgaatt gtatatggtt gatttggatg gtacagcaaa
     4501 agagatagat tactgccaat gtccctattg ggaggaacat aaatattgta agcatacagt
     4561 tgctgtggaa ctttatttac ggtcgaaaaa tctatctcgt atcatgaaaa aagaccagcc
     4621 aatgctagaa ccggtaaaag caacaagtga gggagaaatc attgcaaaag gtttttctcg
     4681 tctaaaaaca caaaacaatc ctcaagcggt gcaacctttg attattgaat accaagtaga
     4741 aacgattgag actaatcagt atcacccaga gctttctatt ttggcacttt acttgcgtat
     4801 cggttcattg gcatcaccga aaaaaacata tatcgtaaaa aacatctatg attttttaca
     4861 agcttacaca gaaaaagaaa cttacactgt taataaacag taccagtttc gtctagataa
     4921 acgggcgttt caaaaagaag atcaaaaaat actcacgcat cttgcaggaa gtgctcagac
     4981 gcatcaatta ttaggtacga acggcttaca agtcaaagga aaactagata aaagatatct
     5041 gcttctccca gtagaacaag cccgcttcct gttagagcag atgaatcaga ctacacgttt
     5101 gcatctgcag ataaatgaac aaaacgatca aggaatacaa ttttccgatc aaaacaaacc
     5161 tttatcattc aaagtggaaa aatttggaga agattatttg ctgcaagcat tgaatgattt
     5221 tgattttttc tttatgcatt atcagtgggg agttatccaa gggacatttt attctctgac
     5281 gaaatatcaa caaacggttt atcaaacttg gaaacaattg attcgccgat tggagaaacc
     5341 agaagtagtg ttcaagaaaa aagaattacc gtctttgttt aaagagatta tccctctgct
     5401 gtcagagatc ggcgacgtag ctattgcccc tgcagtagaa gaggaagtag cagatatccc
     5461 cgtcgaattt attttctttt tccgaaaagc aaaaggaaaa atccaagctc gagtcgattt
     5521 cgtctatggc gaagtcatct attcgactga cccgaagcat gaggtctcga cagaacaggc
     5581 ttttcaggtg ttacgtgaca cagtagaaga gaaacgagta cttgatctat tcagaatgta
     5641 tcgctaccag aaaaatgaaa ctggttatga acgtccattg ccaagcgggg aagaactata
     5701 tgcctttttc cgcacagaac tatctgtctt ccgtcagttt ggtgaagtca gacttggaag
     5761 aaaattgcga gagttgtatt tagatgcgca taaacatcaa ccgaaaatcg aagtaaccga
     5821 gaccggttca tggctcgata ttcgattcga tgtgacaggg atagaggagc aagaaatcga
     5881 tcatgtcctg caaagcttgc tgagaaatga tgctttttat acattagaaa atggtcaggt
     5941 tctttctttt gattcagaag aatttcaaca aacaagccaa gtgttgcaac aattacggga
     6001 atcgatacgt acagaagaag gaacgatcca tgtacctaaa aatcaaggat tgatcattca
     6061 aaatcaattg gaaaaaagca atgctacttt tagtgaatcg ttccaaacga tggtacaaga
     6121 tctcattcat ccagaaagat accaggcaca gcttcccaaa ggattgaatg caacgatgag
     6181 agattatcaa aagcaaggtt ttcgatggtt gaagatgtta ggtcattatc agtttggcgg
     6241 tattttagcc gatgaaatgg gacttgggaa aacactacag acaattgcct ttttactttc
     6301 ggaaaaagaa gaacggaaaa gcttctctgc tttgatcgta gcgcctgcga gcttaattta
     6361 caactggcag gcagaggtga gaaaattcgc cccttcttta agtatccaag taatcaatgg
     6421 gaataaaaaa gaacgtgaag aattactggc taaagacaca gatatccgag tgacttctta
     6481 tgccagctta agacaggatc tagctaatta tcagtcgcaa aaaattgatt atttgattct
     6541 tgatgaggca caaatggtca aaaatagcag tacgaaaact gcacaagcat tgagagaatt
     6601 agctgtacct cagcggtttg cactgagtgg gacaccaatc gagaataatt tagaagaatt
     6661 gtggtcattg tttgcaacga ttatgccagg atttttcccg actaaaacaa aattcaggga
     6721 acttgccgca gaagaaattg cgcaaatgat tcgaccgttt attttacggc gagataaaca
     6781 aactgtgttg aaagacttgc cggaaaaaac agagatgaat ctatattctg cacttacaga
     6841 agaacaaaaa actgtttatt tagcttactt gcgtcaaatg cgtgaagaaa tcacatcaat
     6901 ggattctgaa gcattcaaga aaaaccggat cgggatactt gctggtctga caagactgcg
     6961 acaaatttgc tgcgatcctc gtctgtttat tgaagattac caaggcggtt ccggcaagct
     7021 cgaacaagta aaagatctac tggtagctgc caaagagaac aaacgaagag ttttgctgtt
     7081 ttctcaattt acagggatgc tgactatctt gcaagaagag ttagccgagc tagggatctc
     7141 gacattttat ttaagaggca gtacgaaacc acaagatcgg ttaagtatgg tagacgcatt
     7201 taatgctggc gaaaaagatg tttttctgat ttccttaaag gctgggggaa ctggattgaa
     7261 tctcacggga gcagatacgg tcatcttgta tgacctttgg tggaatcctg ccattgaaga
     7321 acaagctgct ggacgtgcac atcggatcgg acaaaaaaat gtggtagaag tgtggcgtat
     7381 gatctctgaa ggaacgattg aggaacgcat ggattcactc cagcaagaaa aacgcgaact
     7441 gttccaaaaa gtgatccaag gtaatgaaga acagttgacg aaactgactg aagaagacat
     7501 ccgcatgatt ttaagtgtag gagaaatcga ggagtaagag actcgaaaaa gcgttcagta
     7561 atgggtaaga aagcctttct aaagaagcga ttgtttattt gatttaaaaa atagcaagga
     7621 ggctgggatt ccattttatc ccagcttcct tgctatcatt tttttatttc tatttttaaa
     7681 ggttatacaa agattgtctt cattgtctct aacagctgat caaaagaaga aatctcgata
     7741 tcgcaaattg gagtctgttc aattttgcgt tctcgatggt tgaaccaaag cgcttgccag
     7801 ttagcacttt ttgcacctag aacatcattg tcgtaattat cgccaacgta taaggtctct
     7861 tctggaagca tatgaaattc tttctcagct agctgaaaaa tctctttttc tggtttttgg
     7921 aaacctgttg cttgagaaat gagcatattg cgggaaggaa tccaattgtt caattgaagt
     7981 tgcttcagtt tttttgtctg atggtcagtc ggcccatttg tgataattcc taatgggaca
     8041 ttcttttcct tcaagaaatc gagggttttt ttgacctcct catgcaagca gatattatca
     8101 agttcttcct cgtaaatttt ttgaaataag aggccatctt cttccgtaat atgagggtag
     8161 tctaaatctt ttagtgattg gctgatccga tgtgctcgca tatattctag tgtccaatct
     8221 cctgccatta cttttgggaa gttttcatca ctatgatggc ggaaccggat atatagtgga
     8281 tgcatatctg catctgaaac aagcggaacg actcttttga ccgcgtttcg aaatggctgc
     8341 tgctgatcat acatcgtatc atcaacatca aacacaactg atttaatcaa aattttacac
     8401 gcctttcggt tttcattgat tttttcacaa atccttgact attgtaacag aaaaaattgt
     8461 ctaaaaaagc tgataaataa aggtttttct tactatattt ctatttcctt gacagaataa
     8521 ggtttttata tttcagtttt tttcactaaa aatcgtgaaa cacgtcaata agtttgatga
     8581 acaaattaat cattatggta gaagtcgatt gatttatttt aatgcaatct ctgtggaatt
     8641 ttcgaaagat ttattttttt aatacagtct caatataact attcaaacat aaaataacgt
     8701 tatttgattt atttgtttat agtaaatttt atttagtaat cattgcttta aaatactacg
     8761 cttatataac agagaaaata ag
//
LOCUS       DAKBVG010000111         8772 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000111 DAKBVG010000000
VERSION     DAKBVG010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.111"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(136..516)
                     /gene="yajC"
                     /locus_tag="OUD09_001795"
     CDS             complement(136..516)
                     /gene="yajC"
                     /locus_tag="OUD09_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCT9693061.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(613..1758)
                     /gene="tgt"
                     /locus_tag="OUD09_001796"
     CDS             complement(613..1758)
                     /gene="tgt"
                     /locus_tag="OUD09_001796"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCT9693062.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1976..2674
                     /locus_tag="OUD09_001797"
     CDS             1976..2674
                     /locus_tag="OUD09_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HCT9693063.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2842..5481)
                     /locus_tag="OUD09_001798"
     CDS             complement(2842..5481)
                     /locus_tag="OUD09_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HCT9693064.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(6100..7131)
                     /gene="queA"
                     /locus_tag="OUD09_001799"
     CDS             complement(6100..7131)
                     /gene="queA"
                     /locus_tag="OUD09_001799"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCT9693065.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            7324..7929
                     /locus_tag="OUD09_001800"
                     /pseudo
     CDS             7324..7929
                     /locus_tag="OUD09_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(8082..>8772)
                     /locus_tag="OUD09_001801"
     CDS             complement(8082..>8772)
                     /locus_tag="OUD09_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCT9693066.1"
                     /translation="TISRHSSGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSD
                     GQKIDNNKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKV
                     MNQRTDFLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKA
                     DWYGRKIIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILT
                     EGLRLHSMGLA"
BASE COUNT         2489 a         1877 c         1591 g         2815 t
ORIGIN      
        1 attattattt atatgacaca cccgattatt caatattgag caatatatcc catgctctta
       61 tctctttctt ttttgaataa aaaatgaggt aggacatctt tctgccctac ctcgtcttta
      121 aataagcttt gatacttatt tttcttcctt ctcttctgtc tcttccagcg tttctgttgt
      181 ttcagttttt acttcaactg tctcagctgg ttcttctgtt gtttcaacag tagtcgttgc
      241 atcatttgtt acgacagtcc ctggtttgac cgttttgatt gctgcacgat cgtactcaag
      301 gaagatccct tcgcagtcta aaacaactgt ccgtttgtca ttgtcgattt ctgaaacaac
      361 tccatgtaat ccgccgatcg ttacgactga atcacctggt ttcatgctat ctaacaagtt
      421 ttgacgttct tgctgttgtt tcttttgcga acgtgacata aaaaaccaca ttgctaatag
      481 cgcgataaac atcacaagca ttggtaatgt tcccattttt ttcacctcac taattctgta
      541 tctacaaaaa acactttagc agaaaaatgc tgataacact agttttttac ttaattttta
      601 aaagattttc ctttagaagc tttttgcgtt ttctttattg aacccgtatt cttcaaaaaa
      661 ggcttctcta aattcaagca aattatcatc catgattgct tgacgtactt gtttcattag
      721 attcaacaag aagtataaat tgtggtaaga agtcaaacga atcccaaatg tttcatcaca
      781 tttgatcaaa tggcggatat aggcacgtgt atagttttta catgtataac aatcacattt
      841 ctcatcaatt ggacggaaat catgggcata tttcgcattt ttcactacta gacggccttg
      901 agaagtcata catgtgccat ttcgagcgat tcgagtaggc aagacacagt caaacatgtc
      961 tactccacgg ataacaccat cgatcaatga atcagcagca ccgacaccca tcaagtaacg
     1021 tggtttgttt tcggggatca acggtgtagt gaattctaat acacgattca tttctgattt
     1081 aggttccccg acagataacc cgccgattga gtaaccaggg aaatccatcc cgactagatc
     1141 tttcgcgctt tgacgacgaa gatcttcaaa ccctgctcct tggatgattc caaataatcc
     1201 ttgacggtct ggattagcat gagctttcaa tcctcgttct gcccatcgag acgtacgttc
     1261 tactgatttt ttcacgtagt cgtaactttc atcgaatggt ggacattcgt caaaactcat
     1321 catgatatca gagcccaatt tattttggat gttgattgct ttttctggtg ataaaaacat
     1381 acgcgaaccg ttcaagtgat ttttgaaatg cacgccttct tcttcgatat tccgcatatc
     1441 acttaatgaa aaaacttgaa agccgcctga atctgttaaa atcggttgat cccagttcat
     1501 aaatttatgc agtccgccag cttcttctac taagtcttca ccagggcgta gccataaatg
     1561 atatgtatta cttaaaataa cgccagcacc catttctttc aactcttctg gggacattgt
     1621 ttttactgta gctaatgttc ctactggcat gaacattggt gtggggaaag ttccgtgtgg
     1681 cgtgatcaat tcccctaaac gcgcgccagt atgtttttct ttcttgatta aacgatagcg
     1741 aatggcaggt tgcgtcattc atttccctac tttctatata aaatatcttg acaactccca
     1801 ccatcataat cgtttcaagt gctttttgca agttccctcg gcttccttct actttattta
     1861 atattctttt ttttatcctt tttttatgaa taacaaaaaa aagaaaaaca ttatgacaga
     1921 aatatttttt gatacatggt atattggtgt tattaaagaa aaggagggaa aaatcatgca
     1981 atcttctgca gaattaaaac gccaagcaaa agagagtcta aaaggacgtt ggggacaagc
     2041 tgttttacta aatctaatcc ctacattgat tggtattgct actattcttc ttattatgat
     2101 tcctattatc ttgtttgcca ccaccatata tgatggatca gcgagcgtct cagaagcgac
     2161 aacttcagga ggcggtagtt caggaggcgg tggcggaatt atttccagca tcattagcgc
     2221 tttgttcatg agcggtattt cttggactta tcttgatatt ttacgtggac aaaaagatca
     2281 gatcgaacca ttcaaagatg catttcgtgg attcaaaggt ttgtttatcg gcggagtgat
     2341 cagcttagct gtgctgatct cgatctttac aacattatgg ggtttgctat tagttattcc
     2401 tggaatcatt aaaagctatt cttattctca aagctatttt atttactacg atatcgtcca
     2461 agaaacagga caaacaccta aagtactgga tacgatcact gcaagtcgaa aattgatgga
     2521 tggttataaa ggacaacttt tctggttaga tctttcattt atcggctggc atatcttagc
     2581 attagcaact gctggtattg gttatctatg gctcagccca tacatttctg caacaaaagc
     2641 agcattttat gaacaattgc caaaagatat ttaaaagaga gtgtgacttg tgaccgaggg
     2701 ggtggcagga gaacaccgtt tatcgagagg cagtaaaaga aacattcagc ccattagcaa
     2761 aaaggaggtg tgacaacatt catgtcacac ctcctttttg tatagttcca gtaaaaagaa
     2821 aatcaactta gaaacagtta atcattcctc ttcttctgct tccggtaatc gatggaactt
     2881 gatcagttca ttcaccgcga ttggtacgat agataaaatc cctaccaaga accagtgatt
     2941 caaactgatg tgtactaggc cgaatagttc ttgcgtaaat ggcagcaaag caacagccgt
     3001 tgtaatagct aatgccagta acgccatttc taccaaagct ttattggtcg ctaaattgac
     3061 tttgaagatc gattggctgc tgcgtacatt gaacacatgc agaatcgatg aataagctaa
     3121 gatcaagaat gcaacagttt gaccgacatg ctgacttgcg tctacccatg ggctgatagt
     3181 atcgacataa ctacctaaat aataaccaaa caaggttacg atggtgaaga cccctgcatt
     3241 tatggcaatt ttttgcaata aacctcgagc aaagatccct tcatttttcg gaatgggtgg
     3301 ctgatccatg atgccgtgtt ccgctggctc tcttcctaaa gcaaatcctg gaagtccatc
     3361 agcaactaca ttgacaaaca gtagttgcac agctgtaaat ggtgctcccc atcctaacag
     3421 catcgcaatc aaaacaataa agatctccga aatattacag cttagcaaaa agttgatagc
     3481 tttacgaata ttctgataaa ccgaacgtcc ttgactgaca gcgtctacga tcgtcgcaaa
     3541 gttgtcatcc gtcaaaatca tatctgcggc actttgcgcc acatctgttc ctgtgatccc
     3601 catggcacac ccaacgttac ttgctttcaa cgctggtgca tcattcacac catccccagt
     3661 catcgctacg actgcacccg tacgttgcca agatttgacg atccggatct tgtcttctgg
     3721 cgttacccga gcatagacag agagagcttt gacacgcgca tctaattctt cgtctgataa
     3781 ttgatgcagt tctgatcctg aaagtgcttc tgacggatca ttcaagatac ctaattcttt
     3841 cgctatggca ctggcagtca ccacatgatc tccagtgatc atgactgttt tgatgccagc
     3901 ctttttcgct cgcttgattg ctcctttact ttctggacgt ggtggatcga tcattccgat
     3961 cagaccaatc aaacgtaagt ctttttccag tgcttcagaa gaaatatctt gcggttcttc
     4021 gtcataagta gcataaccaa cagcaatcac tctcagtgcc cgtttaccaa agcgatcatt
     4081 aacgatcgcc gcttgttcta catcgcctga actaaagcgc ggagcaagaa catcgaatgc
     4141 tcctttggtg attgagatgt atttttcgcc catttggtga actgttgtca tcatttttcg
     4201 ttcagaatca aaaggaagct cgtttactcg tggatatttt tcttctaatt ctgcctttgt
     4261 atgataattc tcttcaacag cccttacgat ggctacttct gttggattac ctttgataac
     4321 aaggtcgtca tcttcttgct cgaccgtcac atctgtacat agtgcagcca ttttcaatat
     4381 ttccatcgcg ctgttcgtca ttgaatcttc gatattcgtt acttcatctt ctttgctcca
     4441 tacccggcgg acgcgcattt cattttgtgt cagcgttcct gttttgtctg agcagataac
     4501 attagcggtt cctagcgttt caactgctgg caactgtcgg atgatcgcat gtttcctcgc
     4561 catcttctgc acaccgtatg caagtgtcaa cgtaacaata acagtcaagg tttccggtac
     4621 agctgcaaca gccaaggaaa tcgaagtcat gaacatctca aggagtggtt ctccttgcag
     4681 ttcaccaatc acaaatacta aagccgcagc cgctaaagcg atcatactga ttcgtttgcc
     4741 tagctgattc aatcggacct gtaaaggtgt tttttgcata gtattttcgt tcaaaagatc
     4801 cgcgatctta cccatttctg tagccatgcc aatggcagtg acgatcgctt tcccacgacc
     4861 tgcagcaacc gtacatcctt taaataccat attaagttgg tcacctaatg actcatcttc
     4921 ttcagcaata aatgctgcat ctttttcaac tgcttcactt tctccagtca acgccgattc
     4981 ttctacacgt aattgtgatg cttcgatcaa acgggcatcg gcagaaatag catcaccgga
     5041 ctctaaaaca agaatatcac ccactaccag ttgctctgac tcaacagtgg tcactttgcc
     5101 gtctcgtatg acagttgccg tttgtttatt catatcttgc aaggattcca gtgctttttc
     5161 tgcgtttcct tcttggacaa tcgccaatac cgagttgatc acaacgatag cgatgatcag
     5221 cagtccttca aataaatccc catgttctgt agcaaatgcg gtataaaagg aaatcaccgc
     5281 tgctacaaga aggatgatcg ttgtaaaatc ggacaagctt cgaaagaatt ttttgatcat
     5341 cgattctttc ggcgcctcat cgaatttatt caatccctgc ttttttcgcc gttcatccac
     5401 ttcttgtgaa gacaacccag tatctggatc tgcttctgtt accttcttta cttcttctat
     5461 tgtctgcttg tattcttcca tcctattcct cctttaaaat cttcgtacaa aaaaaggtac
     5521 ttattttact ataacatcct ttttttattt acttcaaata aagggcttat cgattatttg
     5581 ttaagaaagc gtaaaattta aagtgcttat atttgcaaac gctaccaaag gatgctatta
     5641 tatagatgta aggaaggagg ataccatcaa atagacagaa gaatttctcg atagaacagt
     5701 gtaagcaaac aaagttgtta gaagtacagg tttcacccag acatgcgtgt agagtacaaa
     5761 cctataaata gtgaatgcgt atggatgttt ggatcacgaa gtatccgaaa caagaagtaa
     5821 tgcgatacgt aacaattgaa taacgtaaac gtctgaaaat tgaataggaa caagagatgt
     5881 ttcaaatgtt gaagatgtaa cgaaaacaaa atttttgttt aacatgaaga aattcaacac
     5941 cgtgctttgc atgtatgatg gaaagttaca atcatttctg tttatttgaa tccttccgag
     6001 gtacaagaaa gccgagctta tcgagagctc ggctttttct ttgtcataaa atctttgtaa
     6061 agcttttttg ttattcaaaa ctagcggctt ttcataacct tatttcacga acatcgcatc
     6121 tccaaaacta aagaaacgat agcgctcttc aatcgcatgc tggtaagcag ataaagtcaa
     6181 ttctcttcct gcaaaggcac taactaacat gactaaagtc gattttggca aatggaaatt
     6241 agttgagaaa gcttctacca ctttgaattc atagccaggc gtaatgaaaa tatcagtcca
     6301 tccgctgtct gctttgattt ctccattaaa tttcgttcca atcgtttcta atgttcgaat
     6361 tgaagttgtt ccgacagcca cgattttccc gccattttta cgtacttggt tcaatcgctc
     6421 agccgcttct tccgtcaaac ggtagaattc actatgcata tggtgttctt caattttatc
     6481 tacgctgaca ggtcggaatg tacctaaccc tacatgtagt gtcagataga ctaattcaac
     6541 accctttgct tcgatctcat caagtaattc tttcgtaaaa tgcaatccag ctgttggcgc
     6601 tgcagcagaa ccattttctt tcgcatagac agtttgatag cgatctgggt cttccagacg
     6661 ttctttgata tatggaggca atggcatttc tcccaatgat tcaagaatct ctagaaaaat
     6721 tccttcatac tcaaagtcaa tgatccgtcc gccgtgttcc aattcttctt tcactactgc
     6781 ttgcaatctg ccatcaccaa aagagattct tgtcccaact ttggcacgtt tcgcaggttt
     6841 gatcaatgtc tcccacgtat ctccttgtgt attcgttaga agaaggactt ctaaatgcgc
     6901 accagtatct ggtttttctc catataaacg agcaggcaga actcgtgtat tattcataac
     6961 tagcgcatca ccaggctgta actcttcaat gatttcatgg aaatgtttat cttccatctc
     7021 tccagttttc cgatccaaaa caagtaaacg agaatgatcg cggtctttta atggtgtttg
     7081 tgcgattaat tcttccggca agtcaaaatc aaaatcttct gttgttaaca tatcgtcact
     7141 cactttctat catgtgcttc atcattctat catgtttttc gttctgttgg caaaatcagc
     7201 actttttcac ttctattttt tgttttccta aataaaaaaa ctaccctatt tgtatttttt
     7261 cattgtttta ggcatactag taatagatag ttcttcattg aaaatagaaa aagaggtggc
     7321 accatgattg aatttcaaaa tgtgtcaaaa atttataaag gtggaaaagt cgctgtagag
     7381 gacgtcaatc tttcatttga aaagggtgag ttcatttgtt ttatcggtac gagcggaagc
     7441 ggaaaaacga cgacgatgcg gatgatcaac cgaatgactg atccaacaca agggaaaatt
     7501 ttgattgatg gaaaatccat ccaagaaatt gatccagtgg aattgcgacg tcagatcggc
     7561 tatgttatcc aaagtattgg attgatgcct cacatgacga tccgtgaaaa tatcactctt
     7621 gtccaaaaat tgttgaaagt cagtcaagaa gaacggaata aaacagctga aaaaatgatt
     7681 gatcttgtgg aattgccccg tgagatgtta gatcgctacc cacatgaatt atctggtggt
     7741 cagcagcagc gtattggagt cgttcgtgcc cttgcagcga atcaagatat catcttgatg
     7801 gacgagccat tcggtgcgct tgatccaatc actcgtgatt ccttgcaaga ccttgtcaaa
     7861 gatttacaag agcgtttggg aaaaacgatc gtatttgtaa cccatgacat ggatgaagcg
     7921 agtgaactac tcaccactta gctaaacctg acggttctta acgcttgaag tgggagcttc
     7981 ttgggaatag agcatacttg atagcgtatt tctttactaa cagcggtgtc tcttatttac
     8041 caagctatcc ccgtagttcc tacggttctt gatgattgga tctaagccaa tcccatagaa
     8101 tgtagtctta gaccttcggt cagaatattg atactagcat tgatatcacg atcatgatga
     8161 gtatgacaaa caggacaagt ccattctcga atttcaagag atttcttgcc atctttatgt
     8221 ccgcactctg aacaaatctg actagacgga aaccatttat ctactttgat gatttttcgt
     8281 ccataccagt cagccttgta ttgtaattta gtcacaaact ttgaccaaga aacatcagaa
     8341 atacttttag ctaatttatg atttcgcaac ataccttttg tgtttaagtc ttcaatacag
     8401 ataatatcgt gatttttgat catttctgta ctcaacttat tcagaaaatc agtacgttga
     8461 ttcattactt tttcatgcaa tctagctact ttgcgttttt gtttttgata atttttagct
     8521 tcaaatagat tggtaccttt atttttagcc aataaagcac gcctagacaa tttgcgttgt
     8581 tcacgcttta gtttcttttc cattttggac gtgaatttat tattatcaat tttttgtcca
     8641 tcagaaagaa tcgcaaagtc cataatacct aagtcaatac caattgtgga attagtttta
     8701 ggtaattcga taatttcttc tttacacaac aaagaaatat agtatttacc gctagaatga
     8761 cgtgatattg ta
//
LOCUS       DAKBVG010000112         8769 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000112 DAKBVG010000000
VERSION     DAKBVG010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8769
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.112"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(377..601)
                     /locus_tag="OUD09_001802"
     CDS             complement(377..601)
                     /locus_tag="OUD09_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693067.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            792..3569
                     /locus_tag="OUD09_001803"
     CDS             792..3569
                     /locus_tag="OUD09_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCT9693068.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            3650..4069
                     /locus_tag="OUD09_001804"
     CDS             3650..4069
                     /locus_tag="OUD09_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HCT9693069.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            4081..4551
                     /locus_tag="OUD09_001805"
     CDS             4081..4551
                     /locus_tag="OUD09_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCT9693070.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            4568..5347
                     /locus_tag="OUD09_001806"
     CDS             4568..5347
                     /locus_tag="OUD09_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCT9693071.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            5334..6155
                     /locus_tag="OUD09_001807"
     CDS             5334..6155
                     /locus_tag="OUD09_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCT9693072.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            6172..7221
                     /locus_tag="OUD09_001808"
     CDS             6172..7221
                     /locus_tag="OUD09_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCT9693073.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            7234..8238
                     /locus_tag="OUD09_001809"
     CDS             7234..8238
                     /locus_tag="OUD09_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCT9693074.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
BASE COUNT         2924 a         1462 c         1816 g         2567 t
ORIGIN      
        1 tcgtttattg tgggtactat cttagaggaa taaataatag tggagaaaac tatggtttgg
       61 cgcagattct atcaggtttg atggaacgaa gacaacctcc cgacatcaag tcaaaagcta
      121 cttgagattt tgatctgaaa ataaaaaaag aaaacaaggt aggaacatca atgtttctac
      181 cttgttttcg taatcaatgg gaaaaaacta agagtggtgt acgtaatcaa taaaaaggat
      241 ggtgacaaca gtacctcttt actctctgaa agcatcataa ctcgtttttc ctcattatgc
      301 caataaattg ttgttatacc acatataagg atttgtaata gctaaaaaca gacaggaaag
      361 ttgaaatctg tgaatcttat tttgtatgga aggatttata gaacgcaaaa atcaagataa
      421 agatgaataa aaatcctgtt tcggctatat attgcgtatc tacgccaata aaacgagttt
      481 gttctgtcat agccatataa agcaaaggca aaaatacaac tacagcgaga taagatccga
      541 tgatttctcg aaaagttttc caggaagtat gtttcctgtt tttatattta tccgatttca
      601 ttgaatagtc atagcctcct tattctttca tatgttgaaa ttccagtata aaaactggaa
      661 ggaactgtac gttttttaga ataaaaatta taagggataa gtaggctgct gtctataata
      721 agatatattt ttcaattctt tattaaagat tttattgaca aaatgaatcc gttttctatg
      781 atggaggagg ggtgaataag ttgttgaaga aggaaatttt atgttactta gagaaccaga
      841 cagccttttt cgatttagaa catatgaacg aggtctttac agcacaaagg ttagctgatc
      901 attttggggt aaaaagaaac acagtcagtc attatttgaa ccagttaaat gatgagcaac
      961 tactagtcaa aatcaattct cgaccagtga tttatttcca caaagaagct tttgaaaaac
     1021 aattttttaa gttacgcacg aatttttatt tttctatcga tgatctcaaa gaggaacagc
     1081 cttttttcgc gcaaccaaaa gacttattct cttttatgat cggatacgac gccagtttga
     1141 aagaaagtat cgaacaaatc aaaacagctc tttactatcc agatggcgga ttaccacttt
     1201 tgattacagg cgaaagtggc acaggtaaaa gttatttagt taatttggtc tatcagtatt
     1261 gtctgcttca tgatctgcta gaagattctg caccattcat cacttttaat tgtgcacaat
     1321 atgcagataa tcctgaactt ttgactagca atttgtttgg acatgttaag ggagcgtaca
     1381 ctggtgcaga agaaaataaa aaaggggctt ttgaagcagc agatggtggg atcttgtttt
     1441 tagacgaggt acatcgctta acaccagaag gtcaggaaaa actgttcacc tatcttgatc
     1501 aaggtgtgat ttatcgtatg ggagaaccta atttgtcacg ccggatcaaa acacgtctct
     1561 tttttgctac gactgaagag attactagtc atttcttaac aactttcatt cgaagaatac
     1621 ctatccgaat cgaactacct gcacttaatc agcgtagtcg taatgaacgg ctagaactga
     1681 tttactcttt ttttctcgaa gaacaaagaa aaatggggag ccctttgtca gtcagtggac
     1741 cagctttgtc acttttaaca agtcagaaat tatccggaaa cataggagag ctgaaaaata
     1801 tcgtcaaagt ttcagcagca aaagcatatg ctgaacagcg tgaacagcct gaaatccatg
     1861 tcacgatcca tcatttgcaa aaagaactac ttgcgcagcc tattactaaa aatacagcac
     1921 agcaaggaat ctatattacg caagaccaga ccttagagca attaatggag aagcaacagc
     1981 ccgaacagca gcgaatcgtt aaaagttttg agcaaatcct aattgatttc aagaaaaata
     2041 actgtctgct atattcttgc gaaggaaaat taaagcaaga agtgattcaa ttatttgatt
     2101 ttttgctgtt tgagaccagt agacaagaaa aacacgagct gctggtttat tggacacaat
     2161 ctatccgaga gacctttcga caaatggaaa ctgcctatca aatcaaattc gatggaaaca
     2221 gtgtctatgc cttgagttac tatctctatc agcgaagcac tatcaaatgg ttaccggaag
     2281 aacatgatat tatccaacta atcaaagaat tagaacaaca agtttctcag tcttatccat
     2341 ctagttatca ctatgtccaa cgaattctgg aattaagtaa gatcaaatta gatattgaag
     2401 acacaagtat ggatcggatt ttactgacga tctatctaaa aaaagcgaaa tggtcaaaag
     2461 aaaaacgact gccaaaagcg atgattgctg ctcacggtta tgcaactgct agcagtatgg
     2521 caaatgtagt aaatcgttta ttacaagagg atctatttga atcatttgat atgccactag
     2581 atgtcacacc acagcagatt gcttcagaaa tccttgatta ttgcgaaaac agcgatgttt
     2641 caaatggatt gatcatatta gtagacatgg gatctctgaa agaaatccat cagtttttta
     2701 aaaaacagtt atccgtaccg cttttgattt tgaataatgt gacgacacct ttagcaatca
     2761 cagtagggga atgcctgcaa aaaaatgtat cattagaaga gatagcagaa gagactgttc
     2821 gacagatcca gccagaatgg cgactgctct atccagagga aaacaaaccc aaagcactca
     2881 ttactacttg cttcactgga atcggtacgg cggtccactt aagtgaactg ctagaaaaaa
     2941 gcttacctac ggcttgtcag ttgaaaatca tcccatatga atatcagcaa ttaaaagata
     3001 agaaaaacag tgacccatta ttttccatat acgaagtcgt aggaatgatt ggcaccacag
     3061 atcctgatat caccaatatc gactatcttt cattagaaga cctcatctcg ggggaaaaga
     3121 tgtctgtact agctgaatgg ttggacacta ctatgaatgc ttcagagaag gaaatattca
     3181 atcagcgcat cattcgtaat ttttcattgg aaaaagtact ggattccgtg actattctag
     3241 atacagagaa ggtgatggga gaaatcgatc attttatgag agaattagag gtgcagttga
     3301 atcgaagcat cagcaatccc aaaaaattag cgttgtatgt ccatgtgagc tgtctcattg
     3361 aacgattgat ccgacaaatg ccaatcgaaa cctatcaagg attagagaaa tggaagcagt
     3421 gtcaaaaaga agggttatct gctattaaat ctgcgtttag tgtcattgaa gagcattata
     3481 gtgtcatgat ccccaattct gagattgcct atatctatga tattttgagt gaagatacag
     3541 agtttctctc gatagaagaa gaattttgaa cataagcaag ttgttgccac taattttggc
     3601 atgcaacttg cttttttagt atgtgtaagt gaaaagagga gatgaaaaag tggatagaga
     3661 aattatctta gcttcccatg gaaaatttgc ttcagggata ttagattcat tagagctgat
     3721 ctatgggaaa aatcattcta ttactgtatt cgattgctat actgatgaga attttgattt
     3781 agcggaaaca gtaaggcagc tttttatgag gtacaaagat aaagaaatca ttgttctgac
     3841 ggatcttttt ggcggaagtg tcaacaacga attcttacaa tatatcaatc gggatcatgt
     3901 ctatctcgtt gccggattga atttgccact attgattgag ctagtatctc ggttggacgc
     3961 tgaagtagag acaactgttt tgatccagca ggtgctaact gattcaaaaa aaatgatcca
     4021 gttttgtaac gagcggctca atcagaaaat agaagaagac gaattttagg aggaagaaaa
     4081 atgattgtat tgacaagagt cgaccatcgg ttattacacg gacaagtagc tttttcatgg
     4141 acacaaacaa taggcgcaga ctgtattttg attgctaatg atgatgtacc tacgaatgag
     4201 atacgaaaga caacgatcaa attagccaag ccacaaggag tcaaactagt gatcaaatca
     4261 attgatgact cgattgccgc gttgaaaagc ggggtgacag ataaatataa gttattcatc
     4321 gtagtggaat cgatcgagga tgcatataag ctggcagaag cgtatccaga aatcaaaacc
     4381 atcaacttag gagggatcaa ggcaaaacct ggtactcgca gcatcggaaa agcggtaaat
     4441 attttaccag aagaggagga acgcttgaaa cgattaattg acaaacaaat agaagtagaa
     4501 atccgtcaag taccagcaga taaaaaagtg gatgtaagag aagttttgta agaaaggaag
     4561 aacctcaatg attgtacaag cagtcttatt aggaattgtt gcatttgtcg cacaatcaga
     4621 atatgcacta gggacatcgt tgctttctcg tccgattgtg acggggctgt ttacaggaat
     4681 cgtgttaggt gatgtaaaaa ctgggatcat tatgggcgca actttagagt tagcatttat
     4741 cggatcgttc tcagtaggtg cttcgattcc acctgatgtt gtgaccggtg ggattttagg
     4801 gacggctttt gctattacag ctggagccgg tacagaaacc gcgcttttac taggtttgcc
     4861 gattgctacg ttgacattga tattgaaaaa tatttactta gggcttttga ttccaattat
     4921 gaaccataaa gcagacaatt atgcagaaga aggaaattat aaaggagtcg agcatatgca
     4981 cttgcttgcc ggtatcggtc tttccttgat gcttggtgtt gtggtaatgg tttcttatat
     5041 ggtgggaagc aatacgatcg gaaatctatt aaatatgatt cctgacttcg ttcaaaaggg
     5101 gttgtcagta gctacagggt tgattccagc tcttggtttt gcgatgcttg ctcgtttgct
     5161 gatcaacaaa caagttgcac catatttttt cttaggtttt gctatagctt cttacttaga
     5221 gattcctgtg accggagtag ctatttttgg tgcgatttta gcagttgtgg tagtcaatat
     5281 tatgaatgtc cgtcaactac cacttcaaac aactgcaggg gaggtagaag aagatgaaga
     5341 attctgagat caaaaacagc aaaataacga aaaaagagct aaatcacgta ttttggcgtt
     5401 cttttcaaat ggagttttct tggaattatg aacgtcagat gaatatggct tatgtttatg
     5461 caatgattcc gattttgaaa aagttatatc agtcgaaaga agaaatggca gcagctttga
     5521 aacggcatct agagtttttt aacacgaccc cacatatcgt tacactgatt ttaggaatca
     5581 cggctgcaat ggaggaagaa aataaggatg atcctgaatt tgatgtgact gcgatcgata
     5641 gtatcaaaac atctttgatg ggacctcttg cagggttagg agattctttc ttttggggaa
     5701 ctttgcgatt gattgccaca ggtgtgggta cctctttagc catgcaagga aatattttag
     5761 gccctatctt gttcattttg attttcaata ttccgcacat tctgtttcgc tatctagcaa
     5821 caggttgggg atatcgctta ggaacgggtt ttttgaaaaa gatccaggca aacggtatga
     5881 tggaaagttt gacattaggc gcgtctatta ttggactgat ggtcgttgga gcaatgactg
     5941 ccacgatgat caccatcaat attcctttaa aaatcggttc aggagaaaat gctgtgactg
     6001 ttcaaagtat ttttgatgat atcgttccaa atatcttatg tcttggagca tttggcatag
     6061 tgttttatct attgaagaaa gaagtgaaac cattaacgat cttgatcgga ctagctttct
     6121 ttggaatttt tggttcgtgg atagggattt tttaaaaaag gagcagagaa aatgtttacg
     6181 atgcaagatt atatctatga agaaaaggaa gtactaagta cgatcttgaa aaagaatgat
     6241 ttttcgacaa gagaacatat gaaaaaaaca gtgaatttac tgatcttagc aacgggttca
     6301 tcttataacg cttgtttagc agcgaagcca gcattggaat cttatggtga tcttactgtc
     6361 gatattcaag aaccttttta ttttttcaat tatggaaaac tttctccttc aatcgatact
     6421 gtgattgctg tgtcacaaag cgggaaaagt gcttctactg ttgaagcagt aaaaatgata
     6481 caaaaacaag ggttacctgt tgttgctatc acaaatgacg tgcaaagtcc acttgcttta
     6541 gaagctgcac aaattattga tttaggtgcc ggggtagaat cagtaggctt cgttacaaaa
     6601 ggctattctg cgactgtttt acagttacta ctcttaggga taggaattgg aataagtaaa
     6661 aataaaatca gcaaaaaaat agaacaagac tacatgcaac agttgcgcaa aataatcaac
     6721 catttaccag cgattattca aaaaacggaa gaattttttg atggatatca aagtttattt
     6781 cgtttagcac agcgtttcgt caccattgga tatggtccaa actggggaac agctaaagaa
     6841 gcagagacga aattgactga aacaatccgc gttccgtcac aaggatttga gttagaagct
     6901 tatatgcacg gcccatacct ggaggcagat gcttcgcatt tgctattctt tattgaaggg
     6961 gaaagtgtaa ataaggaacg ttcgcaaaag ttgcaaagat atatgagtcg gtacgttgga
     7021 gagactctca ccattaccac aaaaaaagca agaaacgaga aaacacttgg attagcaata
     7081 gaatgtgatg aatatctgtc tgtattagcg cttgtcgttc cgtttcaact attcgcttat
     7141 aaaaccgcag tagctaaagg tattgacttg aacaaaaaga tttttgaaga ctttgataca
     7201 gtcttgaaaa gtaaattata agaggtggaa atcatgctga aattcaatga agaagaacaa
     7261 atcaaagcaa taaaaggcgc actagcttta cgtccgcaag tagaagaaat catcgataaa
     7321 ctttatgcgg aaaaatttga cgctgtctat tatctaggaa tcggtggaac gtatgcttcc
     7381 tcgatgcaag ctgtcactta tatgaatggg aaaagcaact tgcctgtctt tgtacagcat
     7441 gctgcagaat attacactac tggaaacaaa agattaacaa aagattctat cgttattttg
     7501 tcctctgtta ccggtacaac tcaagaagta gtgaaagcag tagaacaaat caagaaagta
     7561 ggagcgacac tgattggatt tatcgataaa gcgaacagta agttgtctca gctatgcgat
     7621 tttgtggtca cttatccagc tcctggaaca gaacaaatca aattttttat ggcagcagat
     7681 cgattgatgt atttgaatgg agaattcgaa gcttattcag aatactatga acagctcgaa
     7741 cgttatttac caacagggtt agtggaagca gagaaaaaag cagatgcatt tggcttatca
     7801 tttgcagaaa aacatcgacg tgatagtatg cattatttta ttggagcagg gaatcaatgg
     7861 ggagctgttt attcatatgc catgtgttac tgggaagaac aaagctggct tccatcgaaa
     7921 tctatccatg ctgccgagtt tcttcacggg acgttagaga tcgtagaaga aacaacccca
     7981 gtcacattat ttttaggcga agatgaacag agagtgttat ctgaacgtgt ggctaaactt
     8041 ttgccaaaaa tttgcagcaa ttatacattg atagacacaa aagactatcc agtagaaggt
     8101 atcagtgaaa aatatcgcgg acgtgttttg tctttcttat tgatgcatgt agtgacacaa
     8161 cgcatcgatg ctcatgtgga gcagttgaac tgccatccat tagagatacg ccgctactat
     8221 cgtcagtttg attattagga tagactggat aaaaagaata taaaaaataa agagagcagc
     8281 gataactgct ccctttattt ttttaaaata ttggcatcaa ttacgttata aaacacattt
     8341 tttgcattat ctacagttca acttataatc tgtttaatgg gacatgtggg tgccaaccat
     8401 tttttgttat gcaatagtac tatttagttg ttgcatggat ccgtagtaaa agtccaagta
     8461 caacgagttg cgccagttta catagcgttt tgtatccaga atgttgacta aaacaaaagt
     8521 tcctatgtca accgtcaaca gagacaatct gtccgttgca ggacctgaca gatttagaaa
     8581 tgttcaatct gtctcttatg tattttaaat caatactcac taggagaagt ttcctaacct
     8641 cctaatagta atgctggtag taacattgtc aaaactgctt cctcttagga acaagcaaag
     8701 cataacgagt gtaaaaataa aatgtagact attgtaacat atcattgatt atttcaagta
     8761 aaaacgata
//
LOCUS       DAKBVG010000113         8677 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000113 DAKBVG010000000
VERSION     DAKBVG010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.113"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(75..797)
                     /locus_tag="OUD09_001810"
     CDS             complement(75..797)
                     /locus_tag="OUD09_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HCT9693075.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQSYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKTVPIDHLLIETDGLHAAEWALGGIFSEKYYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(787..1524)
                     /locus_tag="OUD09_001811"
     CDS             complement(787..1524)
                     /locus_tag="OUD09_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT9693076.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYRNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            complement(1536..2528)
                     /locus_tag="OUD09_001812"
     CDS             complement(1536..2528)
                     /locus_tag="OUD09_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCT9693077.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQETKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDITYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDNPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            complement(2543..3286)
                     /locus_tag="OUD09_001813"
     CDS             complement(2543..3286)
                     /locus_tag="OUD09_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT9693078.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     regulatory      complement(3360..3464)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(3527..4849)
                     /locus_tag="OUD09_001814"
     CDS             complement(3527..4849)
                     /locus_tag="OUD09_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCT9693079.1"
                     /translation="MKTYDAIVIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYFVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANVVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRNPPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     gene            complement(4836..5801)
                     /locus_tag="OUD09_001815"
     CDS             complement(4836..5801)
                     /locus_tag="OUD09_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT9693080.1"
                     /translation="MTKLIEFKHVQKKYDRKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFNEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            complement(5798..7336)
                     /locus_tag="OUD09_001816"
     CDS             complement(5798..7336)
                     /locus_tag="OUD09_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HCT9693081.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKVLNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            complement(7752..8039)
                     /locus_tag="OUD09_001817"
     CDS             complement(7752..8039)
                     /locus_tag="OUD09_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693082.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            8173..>8677
                     /locus_tag="OUD09_001818"
     CDS             8173..>8677
                     /locus_tag="OUD09_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCT9693083.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYL"
BASE COUNT         2485 a         1827 c         1486 g         2879 t
ORIGIN      
        1 attaatcgaa cggtttactt ttatacaaag aaagccaaaa agaggtgtga cacaactagt
       61 gtcacacctc ctttttattt acctaatcta atgaaacgtt ccaagttttg ataagtattt
      121 ttcttaactt cttcaactga tagctctttg atctcagcga ttgtctttaa ctgttcttca
      181 agcagtgaga cataatattt ttctgaaaaa atccccccta aagcccactc tgctgcatgt
      241 agtccatccg tttcaataag taagtggtcg attgggactg ttttagctag ttgccagact
      301 gctgggttct ttttcaaatc aagaccaatg ctgaaataac agcctaaatc aatatagtct
      361 tgttgatagt ccaaagaaga ataccaatga atcaaatagt gattagggta agattggata
      421 taatttaaga tctctttttc gcatcctttt gtatgcagta caacaggttt ctcatttatg
      481 gctgcaaaag caagctgtcg ctcaaatact tttttttgtg tagtcatcgg tacattcgtc
      541 caaatattat ctaaccctat ttcaccgatg atccttgctt ttttcaaaaa aggctcaact
      601 tgttcaaacg tatagctatc tgctttccaa ggatggatac cgtaacttaa ggcttgagtc
      661 ttacttgctg caagctgtct gttttccttc cattcttctg gcgaatcaca attgatgatc
      721 gtcaaaatcg tgtgttcctg ctgaaggagt gctaaactct gactcccatc taaatgacaa
      781 tgagcatcac ttaacatcta attcacctgc caataactga taaatcgttt gcttcaactc
      841 tgattgctgc agtagccatt cacgtgaccg tcgtttttca ggcacttgaa tctcagcaat
      901 cacatgtgcc ggacgggcgg aaagcacaag aatcctgtct gataaataaa ttgcttcttc
      961 gatatcatgc gtcacaagta aagtagtccg ctttgctttt ttaggcagct tcaacatcca
     1021 gtcttgcatc tgccctctgg tcatagcatc taacgctcca aatggttcat caagaagcaa
     1081 gtaagaagaa ggattcatga ttgttcgtaa caaggccgtt cgctgcttca tcccgccaga
     1141 taattgttcc ggcaaaaaat gttcatatcc gtttaaccca aagatatcca gataatccct
     1201 tacttgttct tcattgattg ttagatgatt gattttttga tataacgtaa cgtttcgata
     1261 gacgctcagc caaggaagta acatatcttc ttgcggcata taactaaatg ctgtatggag
     1321 accagttact ttttgatcgt ctactttgat cgttccagaa gtcataggct ggactcctgt
     1381 taaagttttc agcaatgttg acttaccggc tccgcttggc gcgacaatcg atgtaaaggt
     1441 atctttaggg atagtgaaat ggatgtcttc taatattttc aggttttcaa tgactacatt
     1501 caagtggctg atttggattg acatatctat cactcctatt ttataaactg attagtgaat
     1561 agttcttgcg aaggtacatc ttgcttgatc aatccgtttt cctgcataaa agctgtataa
     1621 ttgcgccaaa ctttttcttc catcaatccc caattctcag gattatccgt ataatttttt
     1681 gacaagaatg cttgtgattc ttctaaaaac gtccgatcat tttcttttgc atatttttgc
     1741 aacagtttag cactgtcatt tggatcctta atagcttcct gatagccttt tttcgtggca
     1801 tccataaaag actgtaccaa ttctggatca cttttgatca gttgatcatt cgtaataatt
     1861 accggagtat agtaatctaa ccgttcatct aattctttca atgggatata gtttagttca
     1921 atacccgctt cttttgcttt gatcatatcc caaccttcaa agtaccattg gatatctgag
     1981 gattttccta agctttctgg cccttctcca ttgcttccta ccattgtcag ttttgaaaag
     2041 tccccgccgt ctttttccat cacagctttt aagacagctt cttcacttgg actttgccaa
     2101 cctgcatatg tttttccttc aaaatctttt ggtgaatgga tgttactgtc tgatagggta
     2161 gcgaatccag aagtattatg tttgatcact gtagcaattg ctttgactgg tatattctgt
     2221 ccatctgcgt gagcataagt gatatcttct tgataactta caccaaattg tgctttgtcc
     2281 gcacctacta gaccaatact tgtcgaatta tctcctggtt cgatgatttg gacatttaaa
     2341 ccagcctctt tataatagcc tttttctttt gctaggtaga tgccagtatg attagtattc
     2401 ggtacataat ccaaaacaag tgtcactttt ttcaaatcct ttgtttcttg tttgctactt
     2461 gtttcttttg cagtgccaca agcggataaa agcaacagta gcggtaataa aagccataat
     2521 tttttcattt gatccccttc tcttaaaaaa tataaagcat acgataatac ccttttttca
     2581 ataatgaaca agcgccattc agcaataaac tcaatagaat gacacaaatg atcgcagcaa
     2641 aaactttatc gatctggtag ctgtttttta cgcgtatcat gtaataccct aagcctgctt
     2701 gagcacttag ccattctcca acgactgctc cgctgacaca ataagtagca gccactttta
     2761 aaccggaaaa gaagccggat aaacccgctg gtattttata tattcgataa atcttccctg
     2821 tatctgcccc catcgttttc aaaaagatga tttgctcttt tgatactttc ctcaatgtat
     2881 ccgaaaaaga aactacgatc ggaaaatagc ttatcaagac aacaaggatc acttttggaa
     2941 gcataccgaa accaaaccat agggtaagta atggccctaa caccatgatt ggcaacattt
     3001 gtgaaatcac tagtaacgga taaaaagaca tttctaaaaa cttccaataa tccattgcca
     3061 aggcagtaat aaaagaaaga acgctagcta ttaccaaacc aagcactgct tcatttaatg
     3121 tcgtgacaga atgggaaagt aatacttgtg catctgccgc taatgcagaa aatacagcag
     3181 caggtccggg taaaataaag gcaggtattt tccaaactat gacaacaatc tgccaaataa
     3241 ttaaaatgaa tgccatactg actgctggat aaagattttt cttcattttg ttacctcctt
     3301 tcttctaata tgaataacaa aaaacgcttg aaatgggcac gacatagtaa cccgcttcaa
     3361 gcgtctgcta tgtttctccc ttcaccagta ttaactgaca ggttcaatgg gtctaagttc
     3421 acaacttact ctcatgtcgt ctacgacatc cccagaaaca ggtattcatt ttttaaagtg
     3481 tagcacaaga aggaaaaagc aacaagcgct ttagttatca tgctgattat ttcaataaat
     3541 aggagagatc acttgccaat gtcggatacc ccatgatata gctatccaac tcttttttag
     3601 tcaatttttg attgatgact agtgtcaaat catcgatcaa ttcgtctgct tgctcgctaa
     3661 tcactgcagc accaatcaaa taattttgct ggtcaaaggt caattcagcc ttagcaactg
     3721 gttcattgac acgatgatag gtgaaccaac tggtaagatc catctctacc acttgatctg
     3781 acgaagaagc gtgacttttt gtaacaccta cttcagcaag tttaggactg gcgtagacga
     3841 tcgtaggtat aatcggatat ttgataggtt cgcttgttgc atccgttatc cgcttagcta
     3901 cataatttcc ttcaaaagtg gcgactggtg tcaatttggg ggggttcctt gaaacaatat
     3961 ctccacacgc aaaaattttt ggattgctcg tttgcagata atcattgact gcaattccgt
     4021 gtttatcaaa gacgacattc gcttgctcaa gtgctaaaga ttctgtattc ggctgtctcc
     4081 cagtcgcaca aaaaatcata tctgctacta attccgtctc ttttccgact aaacgatagc
     4141 gagtaccttc tgaaatgatc ttttgggttt caacaccaaa agcaaactga atgccagatg
     4201 cttccatttg atggacggct tcctctacca aagacgcctc aaattctttt aatggccgtt
     4261 gattatgatg gacaatggtt actttgctcc ctgcagcatt ggcaatcgta gctagctcaa
     4321 aagcaatata accaccaccg ataaaaatga tttctttagg cagtacaggc aatgataaaa
     4381 agtctgcact agttttcaag tattcctttc cctccactgg taagatcgta ggtctttgcc
     4441 cggttgccaa aacaaagtaa tccgcatgga agacttcttc gttgacttca atagatgaat
     4501 catccaaaaa acgagcggtt cctgaaagat gatcgatttc agcttctgct aattgttttt
     4561 ttcggctttc tgggactgga tccgtaaatg ttcgtttgaa tttttgaagt tcttcccagt
     4621 tggcagttgg gatctcgtta aaaccttttc cactcaattg ttttacccga ttacgtgcct
     4681 ctactgcact taacaagact tttttaggat cacatccacg gttaggacaa gtcccgcccc
     4741 atagatcttc ttcgaccact agtactgttt tacctgcttc tttcaatcca taagcagcac
     4801 ttaagccgct cacacctgaa ccgatgacga ttgcatcata tgttttcatt tccttcctcc
     4861 tctcgactta aaaaagcaaa gatttcttta cttgtcagcg acccaatact tctttgcttt
     4921 tctactatca caatgcttgg atacaccgaa agaagcgaat atacttcttt cataggcgta
     4981 tcaaagcaaa cttgttgacg agcattttct ggtatttcat ttaagaaata tccattgacg
     5041 actaaatctt ctactcgcca ctctttcgtc tgtttcggtt cattaaagaa atgccgaacg
     5101 tagtcgtttt taggattcat caataactgc tctggtgtgt cacattggat aatttctcca
     5161 tccttcataa atgcaatacg atcacctaac ttgattgctt cttccatatt gtgcgtaacg
     5221 aaaacgatgg tcgtgcccaa ttcttcgtgt aaggacaaaa ccaattcttg taatgagttt
     5281 cttgaaatcg gatctaatgc gctaaatggc tcgtccatca aaatcacatc tgggcttgca
     5341 gcaatagctc ggatgatccc gatccgttgc tgttctcctc cagacaactc tctaggcata
     5401 cgatctcgat aaatatcagg agataagcca actttttgaa gaagttcatc tacacggtct
     5461 gctcttttct gcttttccca acctaacatt tcagggatga cttcaatatt ttgcttgact
     5521 gtcatggtcg ggaacaaagc gatttgctgc agcacatatc ccatattcca ccgcattttc
     5581 tgcagattat attcatttaa cgattttcct ttgaagtaga tgtctcctgc tgatggttta
     5641 gataatccat tgatcatttt caaagtagtt gtttttccac taccggaagg accaaccaaa
     5701 acaaagattt ctcctttttt gattgccaga tttaggtcat cgatgacata ctttctatca
     5761 tacttttttt gcacatgttt gaactcgatt aatttagtca ctgatcttcc tcctttacta
     5821 atccttcatt tttcaggaag attcgtgcta cttctgtcgg ctttttcttt tcgacattga
     5881 cttggtaatt catatcaatc atttgatcct ctgttatttt tccgcttaat ttattcagta
     5941 ctttaacgat ttcaggatgt tctttagcaa aatctttttt catcagcggt gcgccttgat
     6001 aagctgggaa taaccctcga tcatcttcta gagtcactag atcgtactcc ttgagctggc
     6061 tatctgtgga ataggcatcc acaatattga catctccatt attgattgct tggtaacgca
     6121 aacttggctc catgctttgt acacttgaaa aattcaagcc atatgcttct tggattcctt
     6181 tatatccatc tgaacgatca ataaattcca gtgtgaatcc cgctttgatc tgatcagaga
     6241 tagctgccaa atcggaaatc tttttaagtt gatgttcgtt cgcaaaagat ctcttgactg
     6301 ccaaggcata cgtattttgg taagccattg gttttaataa tgtcatatcg aattgcttat
     6361 tcaataattc ttctgcttcg tcatatgttt tttcttttgt caaatcagca cttttcattg
     6421 cttcaggtac cttcaccaag ctttctagga cagtacctgt gaactcagga tagatatcga
     6481 tttgaccttg atccagtgca ttgaacaaaa aactcgtctt gccaaaattc ggttttaatt
     6541 caacttctgt ttttgtgttg tcttcaatca attctttata catttggatc aggatttcag
     6601 gttccgctcc taattttcca gcaatcgtga tggtttcatt tttaaacaag gcagactgat
     6661 ataaagaaac gccgccaaat aaacacacca ctgctgctaa agtaatgatg gaatatcgag
     6721 gtttcttatg ctgcaaccag cgaatcagtc cgctgaatat aattgcaagc aaggcagaac
     6781 tgatcgcacc aatcaatgtc aaaacaggtg tatttcggtc aatacctaac aaaataaatg
     6841 tccctaatcc acctgctccg atcaatgctg ccaaagtagc cgtaccgatg attaaaacta
     6901 aggcagttcg gatacctgag ataatgacag gtaatgcgat gggaagctct actttcagca
     6961 agcgtctcat tctggacata ccaaatgcta ccgctgcttc ttcaatagac gggtcaattt
     7021 cagctagacc aatatacgtg ttttggaaaa tcggaagcaa agcgtaaata acaagtgcaa
     7081 tcaatgctgg aacagttccg atccctacaa atgggataag taaacctaat aaggcaagtg
     7141 aaggaatcgt ttggagcaca cccgtgatct gcaataagac ttctgccatt tttttatgat
     7201 tggctgccca aatcgctaaa ggaatagcaa tcagtgctgc aatcagcaaa gaaatgagag
     7261 aaatggacag atgttgaaat gcagcttcca gcaactcgcc ttttctttca cttaatgttt
     7321 gtataaaatt attcatgcga ccttcctttc tgttattctt taagtataaa catttctttc
     7381 ctttttccca actaacttgt cttgccttaa ccttacagca cgaacaaaaa aaaagaaaaa
     7441 aatacctcta aatatgctga caaaaaagaa tccaagcaac aaagtcggat caactttttg
     7501 gttctcgttt tttcactacg aataccataa aaatctatac gaaattgtca cttggaatca
     7561 gctatatctt ttttacacac acaactttta ttttaataaa aagaaacagc caataaagta
     7621 gaaatctcgt gggggagaac acctttattg gcctgttcaa cgaatattat actgtatcat
     7681 gacacttttt tgccaaaaat gtaaaaaacg gttgttttga catcataaac gtcttgtttt
     7741 cttattttta cttatatcac cactttattt tctatctctt tcactccata aagcggaatg
     7801 agttcttctt ttgactgata ataaacagct ttctcacggt tcttcatcaa aaaaagaaag
     7861 atgtctttca tagcaagtgg tgctttgccg tgttcaaata aaaagataaa ttgatttgct
     7921 ttatttatct ggaccattgt cacaggcacc agtttttgtt cttgagccaa aaaaactcct
     7981 tgatactctg tttttttggg tgattttgta agaattgtca ataagtctgc tgctcgcatt
     8041 tttccactcc ttatttgata aataaaaaga aacagccaaa aataattttt tttcattttc
     8101 ctcttgaagt aagagtataa gtcagtataa taaagaagtt gctgaaaatt ttcaaaagaa
     8161 aagaggctag aaatgaaaaa attaaattta tttacagaat tgtgcggaat cattttaggc
     8221 gcaggaatct acggattagc tgtgacagga atcaatattc catctaagct ggcagatggt
     8281 ggggttactg gtattgcttt gctcttgcat aatctttttg gattcgctcc ttctgtcact
     8341 tcactgcttt ttaatttacc tttattgctt atctcattgt ttctttttgg aaaacacgct
     8401 tttctccgga cgatcgtcgg aacatttgcg ctagttttct ttttacattt atgggagagc
     8461 cttcatatac attttgctgt agatagtcta ctgatcaata gtttgatgac aggtattctt
     8521 tctggcattg gttgcggtct cgtctttcga ttcggcggaa gcacaggcgg aacagatatc
     8581 atttatcaag ccattgaaaa atattttcat attaagattg gcaaaagtct tttcttgatt
     8641 acttttggta ttttagttgt ctccttgctt tatttgg
//
LOCUS       DAKBVG010000114         8639 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000114 DAKBVG010000000
VERSION     DAKBVG010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.114"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            <1..1475
                     /locus_tag="OUD09_001819"
     CDS             <1..1475
                     /locus_tag="OUD09_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693084.1"
                     /translation="FGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREYE
                     LVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNLR
                     CLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLLE
                     NLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDDS
                     KESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLEY
                     LNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEWV
                     KICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVCE
                     RLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGID
                     ETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(1551..1886)
                     /locus_tag="OUD09_001820"
     CDS             complement(1551..1886)
                     /locus_tag="OUD09_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCT9693085.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            2013..2228
                     /locus_tag="OUD09_001821"
     CDS             2013..2228
                     /locus_tag="OUD09_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCT9693086.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            2265..2627
                     /locus_tag="OUD09_001822"
     CDS             2265..2627
                     /locus_tag="OUD09_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCT9693087.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGSLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            2673..2999
                     /locus_tag="OUD09_001823"
     CDS             2673..2999
                     /locus_tag="OUD09_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693088.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLS"
     gene            3842..5665
                     /locus_tag="OUD09_001824"
     CDS             3842..5665
                     /locus_tag="OUD09_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCT9693089.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     IWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            5679..7088
                     /locus_tag="OUD09_001825"
     CDS             5679..7088
                     /locus_tag="OUD09_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCT9693090.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            7106..7942
                     /locus_tag="OUD09_001826"
     CDS             7106..7942
                     /locus_tag="OUD09_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCT9693091.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            8007..8525
                     /locus_tag="OUD09_001827"
                     /pseudo
     CDS             8007..8525
                     /locus_tag="OUD09_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2841 a         1313 c         1827 g         2658 t
ORIGIN      
        1 tttttggagg atataagtca gatgcagtga taataattaa tgaagacaac atgttagata
       61 ttactgagct tccgattgat tttaccatga catgttctaa aaattgtcaa agtgattcag
      121 ggagagagta tgaactagtc aatgaaaccg attttgttaa gttgataaaa aatgacgaca
      181 aagaagaaat gaaacagtgg tttaaagact gcctgtttga ttcctccgac atgtctttta
      241 atgagattta tgaaagattg ttaaccattg atatgaataa attacttaag tcaagtaaaa
      301 acttaagatg tctgtttcac aacgatcata atccctctgc tagtattttt accagtaaaa
      361 ctgggcattc cattttttat tgtcattcca gtaattgtga tatcagtgaa aattttattg
      421 gagttgtgat gctaatttta aagaaagaca gtcgggtaga aacatttaat tggctattag
      481 agaacctaga cttgtatcct acacattttc gaaaattgaa agaggaatca aaagtacttt
      541 ttgacactct tgaatcaatg aaagacaaac ttttcaggac aataaggaat tatctagagg
      601 aaatgcgacg tgtctatgat gtcttgcttt ctgatgttaa tttttttgat gatagtaagg
      661 aaagtgtgac ttgtattttg tcgggggaac aattagctaa aaaaatgagt tcttactatg
      721 gtaaaactta tgacatagat aaatgtaata agttactatc acttatgact tttataggat
      781 tgataaaaaa attagatgat gaagatgttc ctttgaagat gctcgaatac ctaaataggt
      841 taaaggaaga gaaagcgtta ggaaacttta atcataagag aagcaatgtc tatcgattag
      901 acgtttttaa tgcaacttct gtgattcaaa aattgaatga agatatcctt ccacttctgg
      961 aaaaaattca ttttacatat caatattttt cttatgaatg ggttaagata tgttttaacg
     1021 aaaaagaggc taaaagagta tttcctcaaa attcccgatc agaactcagc aaggaaaaac
     1081 aattgatttt agatgaagca catgaagttt tacagggctt atcgtggcaa gggacatatc
     1141 taatgacaga gaaagaactt attaacactg tttgtgaacg cctacgtaat gttggggata
     1201 acagcgtgaa aaatgcttta ataaaaaaca gaggctactt tgttaaacaa ggattcactc
     1261 ttgaacgagc ttctaaggat attaagaaac tatacaatgt gaaagcaagt agtagttttt
     1321 taatatattt agcgaaaggg gcagggatag atgaaacaat aaaagaattg atattatcag
     1381 gagctgggat agttcctctg tatcaattta caagtgtaac aagagatgat ggacggagag
     1441 taaggaagag aaaagaccca attgtattaa actagacagg ttatccatag ctcttaatct
     1501 tatttcatct caatacaccc accgttaaag ttcggtgggt tatttatttt tcaatctttc
     1561 aatagctgat tgcgttcatt ttcttgcctt tttaatagct tcatatagtc aattgttcga
     1621 actgcatctt cagtgctaag agaacgatag agagccaaaa actcttcctc ataatctacg
     1681 acaacattgt ctagaaaatg gcttacaggg gttcctaggg ctctagaaag agctgttaag
     1741 ctatcgaggt taggctctgt tacagaattt tcccatcttg ctactgcctg ttgtgtagag
     1801 aagatttttt cagctaattc tttttgtgtc cacccgagag cttttcgctt ttctctaatg
     1861 acctttgcat attgtttctg tttcattaat acacccccat ctcagtatag tacaacaagt
     1921 tgttgtattt gtaatggtat ttgaagtttt ttatatttta tgagttgaaa tacatcatat
     1981 tgttgtatat aataaaacag gaggggagaa tcatgaaaaa agtaagtaat ctcagccgat
     2041 acagaaaaga aaaaggactt tctcaaacgg agttagcaaa aaaaatgaat gttacacagc
     2101 aatgtgtaag ttcgtggcaa acagggagaa cgattcctaa accctatcaa atgaagatgc
     2161 tttctgagat attaagtgta ccaatgaatg agctgttttc tgatgtattt aataaagtaa
     2221 acagttaact aataagcaat tgaatgattt taggaggaga agacatgtat tgtagaaaat
     2281 gtggcaaaga aataacagat gaaagtaagt attgccagtt ttgtggagca ctacaagaaa
     2341 cagatgagac aaccggaaca gacacagtaa agaaaagtac tgagcagaca attaatgaaa
     2401 tgaaggaaca aatcgtacat ggacaagaga cagggaagct gtatttgatt attggttgga
     2461 tttctatggt agtatcgtta gcatttattc ctgttgtatt tggtgctgtt gctgttatta
     2521 tgggctcctt atatcgagaa aaagatgaga aaatgggtac gattttgatg atagcagggg
     2581 tagctggagc aatctttggt gtacttttag ggatgtcaat gtactgataa gagggataag
     2641 agcaaattat tttggagata ggagataaaa agttgaataa agtaaatatg aacaagaagt
     2701 ttgtcattat aggtattgtt gtcttggcta ttataggtat tattttttca aaaatgacag
     2761 gtacatctgg attagagaag tcatcaaaac cattggtaac acagatttta caagaacagt
     2821 acaacttaaa tcgatcatgt gatgatgtga cgattactca tgaaaatgga gataacacat
     2881 accgggctaa agctatatta gacaatggaa gtgctatcaa cataaatatt gagtactatc
     2941 caaagaaaga ccgtatatat gtggagatac catatgctga ggtgttaatg ttaagttgat
     3001 ttaaatgtat tatgcatacc gtccaaacag gaactcccaa aggtgtaaat tcactaggga
     3061 gtttttatag tttcctataa attaaatact aatacaggtg acaaaaaaat attacaatga
     3121 ttgtcgggaa tttttatgtt attcactgtt ataacaatat aaaagaaatg aaaaagaaaa
     3181 aattattatg gatatgtttg gtatttttat tgataattgg tggaattgaa atacttacta
     3241 catgtagatt agaaataaat cagtttaaac atttgctagt taatttttaa gaatactagc
     3301 ccaaatcatt ctatcgatac ctttatatct cggaatgatt gaaattggct attgcttact
     3361 gatttttacc aaaaaaacaa gtatcactat tgttgcctat attgttggaa tgctgtttat
     3421 tccatccttt actcaccaaa ttcatcaatt tttcccaaga gcagaattgt tgaagttgtg
     3481 tgatccgtta tatgcatttt tgatgctttc acgattttgg gaatttcccg ctggttatgt
     3541 aggaatggtt ttactggtat agttaatatt agatatactt attttagcag taagtatacg
     3601 cagatatacc ctagttgatg tgatctaact gttatattac cctatacggt caaacgaaaa
     3661 gataaaaaat taaattccct gttgattgcg ctatcataat gtgctattat tggattacag
     3721 caaaataacg ttattggcat gttactgatt atgcaggcaa gaccaagaca aactaacact
     3781 tcaatgttga ttggggcgtg gtttgacttg gtctttttta ctgctttaag gagagagaat
     3841 aatggattac agagaagtag gaagaaatgt attgcaacag gttgggggaa aagaaaatgt
     3901 ggtcagctta acccattgtg ccaccagact gcgttttgaa ctcaaagata aaaggaaagc
     3961 cgatacgaaa gccttagaaa aaacacttgg tgtaattagt gtggtggata gcggtggaca
     4021 ataccaagtg gtgattggca atgaagtcca aacagccttc aaggaaattc aaaaggaagt
     4081 gggaacttca attaaaaagg gcccctcttc agacaatcaa gaaagtatta tttcaagatt
     4141 gattagtgtc atttctacca cctttacacc gatgattcca gctataacag gtgccgggat
     4201 ggtaaaagcc attttggccg tcttgacttt gactggtgta ctatcagaca gtagtcaaac
     4261 ttacatcatt ttgaatacta ttgctgatgc agccttttat tttatgccag tactattagc
     4321 ctatggggca gccataaagt ttgatgtcaa cccaattttg gcgattactg ttgctggcat
     4381 tttgcttcat ccaaatattt cacagctttt ttctactggc gatgccgttt cttttctagg
     4441 aattccggta ttagctgcag attatgcggg atcagtttta ccgattatct ttactatttg
     4501 gatcatgtcc tatgtagagc cctttgcgga tcgaatttcg ccttcgttta ttaaattctt
     4561 tactaagccg atgctggtat ttttaattat ggggccttta gcgttggttg tcattggacc
     4621 gtttggaacg ttgctgaacg atcttatagc aatgggggct gaatggatta acggcaaagc
     4681 agcttggtta attccatttt tgatgggtgg tttgcagcca tttttggtgg ttacgggtac
     4741 agcttgggca atgacgccaa tcgctactat gcaattgagc agtacgggtt ctgaaatgat
     4801 caatgggcca ggaatgctgg cttctaatat tgctcaatca ggtgctacct ttgctgtagc
     4861 cgcgaaaact aaaaacaaag agttgaagca gctagctgtg tccactggga tcactgcttt
     4921 aatgggaatt actgagcctt cactctatgg agtaaccttg aagctgaaaa aaccattagt
     4981 tgcttccatg attggtggtg caattgctgg tttgtatgct ggcttgagcg gtttagtcag
     5041 atatgccttt gtttcccctg gcttagcagc cttaccagcc tttattggag aaaatccaat
     5101 gaatattgtt cacgcaatta ttacgtgtgt aatcgccttt gttgcaacct ttatcattgc
     5161 ttatctaatt ggatttgacg atacttctga tgaatcagag caaacggcaa tagccgaagc
     5221 agtcattgtt gaaaatccgg taaaaggaac cgtgtgtcct atttctgaag tgaacgatgc
     5281 tgtcttttct aagggactat tgggaaaggg cgtagccatt catccagaag aaaacagcat
     5341 ttattcacct gttgagggag aagtcattac tattttagaa tctaaacatg cgattggtct
     5401 acgttctaaa gaaggtattg aattattgat ccatgtgggg atcgacaccg tgaatttaaa
     5461 aggggaaccc tttgaagtat ttgtaaaagc aggagatcag gtgtcagcag gacaattatt
     5521 aatcaacgcc gatttcgctg ccattagaaa cgctggctac gatgatacag tgattgtggt
     5581 agtgaccaac agtgaaaatt acgcaggaat tagtgaagca agcggaagct taaaggaaca
     5641 cgaaccattg atgcaaatta gttaaggaga ggatttttgt gcaatacaaa cagctttcag
     5701 catttccaga gaagttttta tggggtgcct ccattagtgc ctaccaggca gagggtgcct
     5761 atcaggagga tgggaaacaa ccgtctatta ttgacaagta tcagcataca gaaggggtag
     5821 ctaattttga agttgcctcg gatttttacc atcactataa agaagatata cagctgttta
     5881 aagaattagg cttaaaagct tttcgctttt ccatcgcttg gacgcgaatt atggatattg
     5941 aaaataataa aaccaatcaa aagggaattt ctttttatca tcaagtaatt gatgaatgcc
     6001 tgaatcaggg aatcgaaccg attgtcacca tgtaccattt cgatttacct tattttctag
     6061 aggagcaggg cggttggtta aatcgagcaa ctatcgatta ttttgtggac tatgtacagg
     6121 tattgcttac tgagtatggt gacaaggtga actattggct gacaattaat gagcaaaaca
     6181 cgatgatcct tcatcctgga gcgattggtt tgccaccaag tggagagctg ccttcaaaaa
     6241 aagaactctt tcaaattaat caccatgttt tattagctca agctagagta attcacttgt
     6301 atcatcagtt gaatcttaaa ggaaaaatcg gtccagcaat caatttaacg gctatgtatc
     6361 aagctacctc cagtccagaa gatgcaattg cagcccataa ttgggaaaca ttgagaggct
     6421 ggtctttctt agatgtagca gttcggggaa aatacaatta tttgtttgaa aactatttga
     6481 atgatcgtgg attatatcct aaaattgagg cagaggatca ggcaatttta tcctccggta
     6541 aacccgattt tattgcgatt aattattatt caactgccac catcgctgcc agcaaaaatg
     6601 acggatcaga tgtttccgct agagcggggg atcaacagat catgttggga gaagaaggtg
     6661 tctaccgggc agctgaaaat ccttatgtag ataaaacgcc atatggctgg gtagttgacc
     6721 ctactggact acgattaaca ttgaggaaat tgtacgatcg ctatgattta cctattttaa
     6781 ttactgaaaa tggctatggg gcacccgaca tagttgagga agatagacga attaatgacc
     6841 aggatcgaat tgattatcta gccaaacata ttctggctat tcaagcagct ttaacagatg
     6901 gtgtagatgt ttttggttat ttaccttggt cagcgattga tgttgttagt acccatcaag
     6961 gcttcaataa gcgttatggt tttatatatg ttgaccgaac cgatgagaat cttaaagaat
     7021 taaaacgaat aaaaaaagaa agcttctttt ggtatcaaaa agtaatcgca agaaatggct
     7081 tagcttagag agaacggtga atctaatgag agtaattaaa acgttaaaca acagtttaat
     7141 tctttgctta gataagcaag gcaaagaagt aattttgatg gggaaaggga ttggatttca
     7201 actaaagaaa ggcgatttgg ttccggctga caaaatccaa aaagtattta aattagattc
     7261 actttctgat gctctaaaaa aggattacat taaactgttt gaggctgctc cggatgatct
     7321 aattatttgc gtcaagcgaa ttatcgatta tgccaacgcc aagtttgact ttaagctgaa
     7381 taaaagtcta ttttttacct tggttgatca cttgaagcat gccattgaac gcagtcaaaa
     7441 aggcattgtt tttcaaaatc gcctgctgat tgaaattcaa aagtattacc caaaagaatt
     7501 tgagatcggc tgctatgcta tccaattgat caacaaaaaa atgaatgttc agttgccaga
     7561 agaggaggct ggaaacattg cgtttcacat tgtaaatgcc caaagtgaac aagcaaatat
     7621 ggaagaaacc atgctctcgg taagagcact aaaggatatc ttggctatgc taggatatct
     7681 gtttcccgaa aaaaaggttg ataaagattc gatgctttac ttaagatttg tgacgcattt
     7741 acagtttttt attgcccgaa tgattaagaa ggaagagttg ccggagaagg atgatttttt
     7801 gctggcttca gtgaagcagc gttttccgaa agagtatcgg gcggccttga aaatcaagga
     7861 gtacgttcaa gaagagatgg gtgcctcagc aaatcaggac gaactgcttt atctaacact
     7921 gcatatttca agggtgtact gaatgaagaa aatatcggct gcacaatata taaagacttg
     7981 ttggttgtgc taaggctcct gattccttgg aaaattcttc taacagtgcg gaggtgaatg
     8041 tttcgacctc taccagtgaa gtggtaaagt gttcacaaga aactactagc ttgccagaag
     8101 agctacttgg agattggcag attaataata aggatgctgg tatagagata tcagacagta
     8161 aagctgtatt ccgtatagga aggtctcaag tagagtcaaa ggattattat gtagatgtaa
     8221 acaacaaaat gcttttacat gtctattttt cttttgagta tgatagtgtg aaatacattg
     8281 aagtctctat tgaaagaact ggttatagtg aatttattag taaaagcgtg ggaattttaa
     8341 ctatgcacac taaagatgga caatatataa gtttgggtgt gaagagagtt tccggtgaga
     8401 ataatgagac agaagcagtt acggagcaga cgatggatga aagaatcgat tcatctaaga
     8461 gtaatgagac agaagtagat actgggaaga cgatagatga aagaattgat tcattcatcg
     8521 aaactggtgt tagtatcaaa tcttagacag cttttcgtag agacaagaat aaattaaact
     8581 tatctacgag aggatgaatt tttatgaccc gatatgaaga aaattttaaa caaatgatt
//
LOCUS       DAKBVG010000115         8620 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000115 DAKBVG010000000
VERSION     DAKBVG010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8620
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.115"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(122..2503)
                     /locus_tag="OUD09_001828"
     CDS             complement(122..2503)
                     /locus_tag="OUD09_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HCT9693092.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2493..3140)
                     /gene="recU"
                     /locus_tag="OUD09_001829"
     CDS             complement(2493..3140)
                     /gene="recU"
                     /locus_tag="OUD09_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HCT9693093.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3204..3743
                     /locus_tag="OUD09_001830"
     CDS             3204..3743
                     /locus_tag="OUD09_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HCT9693094.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3833..4252
                     /gene="gpsB"
                     /locus_tag="OUD09_001831"
     CDS             3833..4252
                     /gene="gpsB"
                     /locus_tag="OUD09_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HCT9693095.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4274..4641
                     /gene="rnpB"
                     /locus_tag="OUD09_001832"
     ncRNA           4274..4641
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="OUD09_001832"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4850..6016
                     /locus_tag="OUD09_001833"
     CDS             4850..6016
                     /locus_tag="OUD09_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HCT9693096.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6062..7558
                     /locus_tag="OUD09_001834"
     CDS             6062..7558
                     /locus_tag="OUD09_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HCT9693097.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7639..8322
                     /locus_tag="OUD09_001835"
     CDS             7639..8322
                     /locus_tag="OUD09_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCT9693098.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
BASE COUNT         2704 a         1572 c         1833 g         2511 t
ORIGIN      
        1 atatgctgga tagtcttata aaaaatcatg taatacagct tggtacatga tttttatttg
       61 tagctgtttt ttacatagac acaaaaaaga aaccacgctt gtagacgtgg tttctttttt
      121 actatctgtt tccagaagct gaggtgctgc tggatggtgt atggtcattt gcagcatccg
      181 ctccgctagt tgctggctca ctggaagaag caggaggagc agatgaagac ggtgtcgagc
      241 tttcttcgga tgaagaagaa ggaaccgttg ttgaagacgc tggctcgctt gtttctgctg
      301 tcgtgctttc ttttgaggac tcagcagttg attcgctttg actagtagag ctagagcttt
      361 gagtcgttgt ctctgttgtt gagcttccag gagtctggac atagctggaa gatggaatag
      421 ttgttgatgg tgtaattgca ttcgatctag ctgtatattg atctttgtag tacagttcac
      481 cgcccacacg aattaatccg cttggcatct cccagtctgt atttgtcacg ttagctgaaa
      541 catactgcat aagctcgcga taaacatcag aggctacgtg tgacgattct gaagtgacag
      601 gtgtcatttt tttattgtaa cctgtccaca cggaaatcga ataatttggc gtatagcccg
      661 caaataggat atctggatac accccagaag aaataccaag ttttgcatac tcgtcatctg
      721 tgtaattgga tgttccggtc ttaccggctt gatataaccc agcgatttgc gcattcgtac
      781 cagttccttc agtgatcgta tcttttaaaa tgtctgtgat catataagct gtttctggag
      841 acatggctgt ttttccatct ggctcataag tttcttctgt tccgtcttgg aagacgattt
      901 tattaacata ttgaggtttg taataagtcc ctccattggc gaaagccgca taggctgctg
      961 ccatttttaa ggaagaagct ccgtattttg tcccatcttg ttcttccgta ttactagaga
     1021 tcgcattcga ttgatggatc gtactgtatt cgatcccaag attttttaag aaagaagcca
     1081 ctttatctga accgacttca ttgaataatt tgactgctgg aacattacgg gataagtaaa
     1141 gtgcttggcg taaagtaatg gtgcccatat attgattatc ccagtttcct acaggagtcg
     1201 aagtcccttc atagttatac ggtgcgtcag taatagtttt tccagtagaa tatttcaaat
     1261 attcaaaagc tggtccgtaa tctgtgacag gtttcatcgt agaaccaaaa tcacgggaag
     1321 tattcactgc taagttgttg cctaaagtta cgtcttctgc gatgtgacgt ccgccaatct
     1381 gggcttttac tttcccggta ttggtgtcga taagggtgga ggcaacttgc atctcgtcgt
     1441 ctgggtaaga gacatactga tccgtgttga cgatatcata tagtttcttt tgggcgtcca
     1501 gatccaaatt cgtataaatc tctaaaccgt ctgtgtagac attcttatct gttttttctt
     1561 gtacttcatt gatgacttct ttgacataat tatcgacgat cttagtattg tcatcggatt
     1621 gagttagttc ttgcagacca tccgttaccg gtacattaac agcttgatca tattctgttt
     1681 gagagatttt ttcgttttga agcatcgtat acaaaacagt atctcgtctt ttcttcgcct
     1741 gatctggata aacataagga tcataggcgg aaggggcttg aggcatccca gcaagcaatg
     1801 ctgtttgcgg taaagaaagt tctgataatt tcttaccaaa gtacatctca gaagctgtct
     1861 ccatcccgta caagccgttt gacatataca ctttatttac gtagtaggtc aagatctctt
     1921 gtttcgattt tttttgttcc aatcttacag ccatccacgc ttcttgggcc ttgcgtttca
     1981 gagtctgatc ctcggcactg gtggagaaga aggaaagttt gatcaattgc tgtgtcaaag
     2041 tacttccgcc ttgcagtccg ccagaggtaa agttagagag ggcagaaccg atgatccgaa
     2101 tagggtcaac cccgatgtgt ttataaaaac ggcgatcttc taccgacacg atcgcatctt
     2161 ctaatgtttt cggtaattca ttcgcagaaa ttttttcacg cttttctgcg cctaaatctt
     2221 cgaatagttc gccatcttgt gtatatagtt tggaagatac ggtagcgtct aattttttat
     2281 ccgtcaattc gggcgcatct tttgcgtaat accagaaaag tcctacacct gctaaaaaga
     2341 ggatacagaa aaatgagagt attcctaaaa ggatcttgat aaataatcct tttttatgtg
     2401 ttccagagga ctttccagaa cctttgccag aattcttctt cacactcttt ttaggagtag
     2461 gctgtttctg tcgtctagag cttctagttt gttcatttgc cattcttgct ttctccttca
     2521 atagataatg ttgataaata gaagtcaacc gcgtccaaat aaggaatccg cggagaaata
     2581 ccagttttga tctctattcc tagttcttcg atcaaagata aaggtagtga tttgcgtcct
     2641 ttttcctttt gggcgtccca gtaagcaatc aaatgaacac tattcaaata aaaacagcgc
     2701 ttcaaagacg aaaaccataa caagacaaag cagaccccat tttgttcgag gcatgctttc
     2761 atatgttcga tttgatgggc atggaagttt ttaaagggaa aagatgtttt gttcttggtt
     2821 tcttttgctt caaagtccaa gtaaaagcct cgatagacgc cattatagtc tgtagttgag
     2881 gcttgggtaa agtaggcttc cttgatcaca gcagcacttc tcttaggata gtctactttc
     2941 acgatttgga taggggtagg ctttttatgg ataaccgcca acccgcggga aagataatat
     3001 ttattacttt cattgattgc ttcttcaaag cgcatccccc gattgccgaa ttcagtgatc
     3061 tgatttcttt tttcttgaat tttttcgacg ggagaggcat tcttataata aggttggcca
     3121 tttggatagc gcaataccat ctttaatcac actcctaggc cacattatag caaaaaagcg
     3181 gatagaaaga aaaggaatct gagatggata agtcaaaagt aatctatgtg tctgggtatc
     3241 gaagtttcga actcggtgtt tttaaagaaa atgatcctaa aatccaagtt gttaaaaatg
     3301 ttttaaaaaa agaactccat cagttagcag aagaagggct ggaatgggta cttgtcagtg
     3361 ggaatttggg cgtcgaacta tgggctgtgc aaatagtagc ggaattgaaa gaagaatatc
     3421 cggaactatc tcttggtatt ttgtatcctt tttctgattt tggtaataac tggaatgaag
     3481 ggaaccaaga aaaaaaacaa ttagcagaac aattagcaga ttatgtcgaa gctgttagcc
     3541 atcaacccta ccaatcacct agtcagctaa agaatcacac tcgttttata cttgagcata
     3601 cagtgggctg tctgctgatc tatgatgaag aattccctgg aaaaacgaag tatttttttg
     3661 aagatgcaca gaattaccaa gcagatcatc cctacgaaat tcggctgatc agtatggacg
     3721 atcttcaaaa ttttagtgaa taatcgatat tgtgtttgat ttatcaactt ttttcctata
     3781 caatgaaaaa ggtagaaatg ttattacgta aatgaaaact agaggtgtaa atatggcgaa
     3841 tttggtttat agtcctaaag acattttaca acaagaattc aaaacaaaaa tgcgcggcta
     3901 tgatcccgtt gaagttgatg aattcttaga caatatcatc aaagattatg aaacttacag
     3961 caaagaactc cttgctttac aagaagaaaa cgatcgattg agtgcaaaag tagctcaatt
     4021 atctaaaact caaggagcag ctcaaacacg cgtgcaacaa acagaggcac caaagagcgc
     4081 agcagtgacc aattttgata tcttaaaacg cttgtcaaat cttgaacgtg aagtatttgg
     4141 taaaaagctt gatcaacaag caagtgcagt caaaccagca caacctaatc caaataacta
     4201 cacaaatgca gacacaagct tagatgataa tgaaaaaaca cgtcaatttt aatttttgat
     4261 taatgaattt gcggttttcg ggtaatcgcg gtctgacttg atcaggctga ggaaagtcca
     4321 tgctcgcaca ggctgtgatg cctgtagtgt tcgtgcttag cgaaaccata agctaaggta
     4381 ctctttggag taacggcagg aaaacaagct aaggcttcgg ctatgcttaa gtatccttga
     4441 aagtgccaca gtgacgaagc ggctggggaa acccatccgg tggaacgcgg taaacccctc
     4501 gagcgagcaa cccaaacaat ggtaggggcg ctttttgtct ggaagaagaa caggacaaaa
     4561 aggcagtata actgcagata gatggttacc gctttgtttt ttctccctgg aaaagcaaag
     4621 tacagaacat ggcttataga aaaccgcaaa catcagggaa ttaggtcgtg acaaaagtaa
     4681 tgcgttataa gaaacaagaa aaacatttga aagctatttt tcaatacttc agatgaatcg
     4741 ttcttattac aaaacaaact tttgtatcac cggttattcg gaatttgagt aaaagtgtgg
     4801 ggtaggactg atgtcccacc tccttttttt ataaataagg agattgtgta tggaaaaaaa
     4861 caggaagttc aacctcgtcg caactgcggc aagcgggcta gaagcactgg ttggtaaaga
     4921 attaagagat cttggaatcg aatgccaagt tgagaacgga cgtgcacgtt ttgaaggaac
     4981 aatggaaacg atcgctacag ctaatttatg gttacgtaca gcagatcgag taaaaatcgt
     5041 ggttggggaa ttcgatgcgt atacgtttga tgaattgttt gaaaacgtaa aagcattgcc
     5101 ttgggaagat tttcttccac tagatgcagc ttttcctgta gctggaaaat cgatcaagtc
     5161 gaaactatat agcacacctg attgccaagc gatcacaaaa aaagcaatcg ttgaacgtct
     5221 ccgtacctat tatcaccgtc cagcttctgt accactgacg gaaactggcg cacattttca
     5281 gttggaagta gctttgctga aagaccatgt gatggtaact ctggatacga caggaccaag
     5341 tttattcaaa cgtggttatc gcttagaaaa aggtggagca ccattgaaag aaaacatggc
     5401 agcagcactt attatgttga caaattggcg aaaagaccgt ccattttatg atcctgtatg
     5461 tggttcaggt acgctttgta ttgaagcagc cttaataggc cataatatcg caccaggatt
     5521 caatcgtgag tttgcttgcg aatcttggga ttggttcagt caagaaatca tggaaaatgt
     5581 tcgatcagca gccgaagaaa aagcagatta tgatattgaa ttagatatta ctggttcaga
     5641 tatcaatgga cggatgattg aaattgccaa agccaatgca gaagaaatcg gattaggctc
     5701 ttcgattacc tttaagcaac aagcagtcaa agacttcaaa acggataaag aatacggggt
     5761 catcgtggct aatccgcctt acggagaacg tctaggagaa gaagaaactg ttcggaaatt
     5821 atataaagaa atgggcgatg tattccgtcc attaaaaaca tggagtaaat atatcttgac
     5881 tagcgactta gcgtttgagg aattttacgg acaaaaagca accaaaaaaa gaaaattgta
     5941 caatggtgca ttgcgtacgg atctattcca gtattgggga actcgtccgc ctagaaaaaa
     6001 agagaaagac gaataatctt actaattgat tcattcaatt ggatgtttgg aggaataggg
     6061 aatgaaagaa aaagagtttt tacaagaagt aaaagaaatc aatcttctac aacaagcttt
     6121 aggaatcctc gattgggata cacaaacagg tatgcctgaa aaagctagtg cataccgaag
     6181 tgaagtagac agctatcttt acagcattta tttttcaaaa aaaatcggtc caaaaatcaa
     6241 agaagccatt cgctattttt ctgaacatcc ggaagaattg tcggaagttg gaactgccgt
     6301 ttttgccaaa gtaaaagaag agtatgattt ggaacatcgc gtaccggaag atcttatgag
     6361 aaaatataca gctgccgttt cttcagcaca cggacaatgg caaaaagcta gagaaacaca
     6421 acaattttct gattttcaac acgcattgaa tcaaaatatt gaattaacaa aacagttgat
     6481 accttactgg agaaaagatg aaaagacgaa ttatgatgtt ttgctgaatc aatacgaacc
     6541 cgggatgact gtcgaaatcc ttgataaggt atttgatcag cttaaagaag gaattctttc
     6601 gatccgccgt acgctccaag aaaaagggac tgaacctgaa acagactttt taaatcggag
     6661 aatgacgaaa aaacaacaga agagatttgt agtgaaagta atcgaacaac ttggttatga
     6721 ttttagtcgt ggtcgtttag atgacactgt ccacccattt atgactggaa ttaatccgaa
     6781 tgatgcacga atcacgacac gatggaacga aacagatttc aaaatggctg tttttggagt
     6841 gatccacgag gctggtcatg gtatgtatga acaagacatc gatgataaat atgcgtatac
     6901 accggtttat gaaggtacat caatgggtat ccatgaatca caatcgttgt ttaacgagat
     6961 cattattgga agtagtcgca gcttctggaa aaagcaatat cctttctttc aagaatgtgc
     7021 agaaggaaca ttcgatgata ttccttttga caaattttat cgttcattga accatacaaa
     7081 atcaagtttg gttcgtatcg aggcagacag tttgacttac cctctccata tcatcattcg
     7141 ttatgaaatc gaaaaaatga ttttcaatga aggggtagat gttgctgaac tacctgaaat
     7201 atggaatcaa aaatatgaag agtatctggg aatacgacca acaaatgatg tggaaggcat
     7261 cctacaagac gttcattggt caggtgcaag ctttggttat tttccctctt atgcattagg
     7321 gtacatgtat gcggcacaat tacttcattc tttgcagcaa gaaatagatg tggaaaatgt
     7381 tctagcgtca gacgattatt ctccaatcaa aaactggatg tcagagcata tccatcaata
     7441 tggtgcatct agaaaaccaa atcaattaat catggatgca acacatgaac cgcttaatcc
     7501 gcaatacctg atcgactata tgaaagacct gtattttaat gtatatcagg taaaataaaa
     7561 tacaatttac aaagatagct gaaattcatc taatctatag atgttttcag ctatttactt
     7621 aagaaagaag tggaacgaat ggataaatta caagtgatcg ctgattatag tatggaccaa
     7681 ttagcgcatg atcaaacagg acatgggagc gatcatacca aaagagttgt caagttagct
     7741 gaaaaaattt tggagacgga accgcaggca gaccgctttg tgacgcttgc agcagcttac
     7801 ctacatgata cgattgatga taaagtcgtt aaaaatgaga acgaggcaaa gcaacagctt
     7861 cgtgtattcc ttcgctcttt gccaatagta gaggaacaga tctcgatgat tttttccatt
     7921 atcgaaaata tgtccttttc taaaaatctc tctgaagcgg ccgaactatc tctggaaggt
     7981 aagatcgttc aagacgctga tcgtatcgaa gcattaggag caatcggcat tttgcgtact
     8041 gcttatttcg gcggtggaca tgggcacccg atttttgatc ctgaactgta tccgcaaaca
     8101 ttcaaggaca aagaaaatta ccgaaaaggc acgacagtga tcaatcattt ttatgaaaaa
     8161 ctgttcttgc tgtctgataa aatgaatact gattacggat atgaagaagc caaacgaaga
     8221 gaaaccttca tgcgtgaatt cttagaagaa ttttttctgg aatggtctat tgacgatgaa
     8281 aattttactc cagacaattg gatcaaaaat aacaaaaaat gatggaggtt gtgacagaag
     8341 tggtcagctc caagatattt cggatcattt tccgtaaaag ttacttctgg aacactgttt
     8401 accaagaggt cgtgagcctg ccgttcagct ccgagtcaca aggaacaatt tcaaaaaata
     8461 gcttctcgtg tttgatgaat attgtaactt gtgattcgga attggcagga gaacaccgtt
     8521 tattcggaag aataggactg tgacaagact tttgtcacag tccttttttc gaattcaaca
     8581 agtaaaactt ttaagaaata ttcagagagt aaaattaaaa
//
LOCUS       DAKBVG010000116         8531 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000116 DAKBVG010000000
VERSION     DAKBVG010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.116"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            211..2820
                     /gene="clpB"
                     /locus_tag="OUD09_001836"
     CDS             211..2820
                     /gene="clpB"
                     /locus_tag="OUD09_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCT9693099.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(2971..3657)
                     /locus_tag="OUD09_001837"
     CDS             complement(2971..3657)
                     /locus_tag="OUD09_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693100.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(3635..4129)
                     /locus_tag="OUD09_001838"
     CDS             complement(3635..4129)
                     /locus_tag="OUD09_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HCT9693101.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(4149..5369)
                     /locus_tag="OUD09_001839"
     CDS             complement(4149..5369)
                     /locus_tag="OUD09_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCT9693102.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            5516..7351
                     /gene="lepA"
                     /locus_tag="OUD09_001840"
     CDS             5516..7351
                     /gene="lepA"
                     /locus_tag="OUD09_001840"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCT9693103.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(7445..>8531)
                     /locus_tag="OUD09_001841"
     CDS             complement(7445..>8531)
                     /locus_tag="OUD09_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCT9693104.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT         2743 a         1622 c         1709 g         2457 t
ORIGIN      
        1 aacgttatgg ataagataat tttacgtttg tttggctctt tttttatata catacaatca
       61 attttgtttt tttaagattt atataagtcg agaaaaaaag tcaaaaaact atgtgtatat
      121 tttgaccaaa attgaccaaa gcgatataat agtttttgta aagatcaaag aaataagtga
      181 tcttaccttt aattaggagg gtatacctgt atgaatattg agaaaatgac tacgacgctt
      241 caagaagcga ttgctgaagc tcaacagatt gcggtcactc gaaaacatca agatatcgac
      301 attgcccatg tatggaaaat ctttttacaa tctaatcatt ttggtcgaaa tttctatacg
      361 gatgccggat tggatgtcga atcttttgaa catgaaattg atcgtctact ggatgagtat
      421 ccagttgttt ctggtggaaa cgtccaatat ggacaaaacc tcagccagaa tttctttcga
      481 ttattgaatg aagcagacca aatacgtgaa agcttcggcg acgagtttct ttcaacggaa
      541 gtcgttattt tagcgttgat gaaattaaaa aattatccgt taactgttta tttgaataaa
      601 aacggattat ctgaaaaaga attgcgtaaa aacatcgaag agatgagagg aggagaaaga
      661 gtgacctcac aaaaccaaga agaacaatac aaagcattag aaaaatacgg cgttgattta
      721 gtacagcaag tcaaaagcgg aaaaatggat ccaatcatcg gtcgtgatga agaaatccgt
      781 gatgtcatcc gtattctttc acgaaaaaca aaaaataatc ctgttctgat aggagaaccc
      841 ggtgtaggga aaacggcaat cgttgaaggg cttgctcaac gtatcgtacg caaagatgtg
      901 cctgaaaatt taaaagataa aacgattttt tcattagata tgggtgcatt gatcgctgga
      961 gcaaaattcc gtggtgaatt tgaagaaaga ctaaaagcag tcttgaaaga agtcaaaaaa
     1021 agtgatggac gtatcctctt gttcatcgat gaaatccaca atatcgtagg ggcaggaaaa
     1081 acagaaggaa gtatggatgc cggaaatctg ttgaaaccaa tgcttgctcg cggtgaatta
     1141 catttgatcg gtgccacaac tttagatgag taccgtcaat atatggaaaa agacaaagcg
     1201 ttggaacgtc gctttcaaaa ggtattagtc aaagaaccaa cagtggaaga tacgatcagt
     1261 attctgcgtg gattaaaaga acgttttgag atccaccatg gtgttaacat ccatgacaac
     1321 gcattagtag cagcagctac tttatctgac cgttatatta ctgatcggtt cttaccggac
     1381 aaagcaatcg atttgatcga tgaagccagt gcaacgattc gggtagaaat gaactctatg
     1441 ccaacggaat tagatcaagt tactagacgt ttgatgcagt tggaaatcga agaagctgca
     1501 ctgaaaaaag aatcagatga tgcaagtaaa aaacgcttga aaaatttgca agaagaatta
     1561 gctgaacttc gtgaagaagc aaatgcgatg aagatgcagt gggaaacaga aaaagaagaa
     1621 gtcaattctg tttcagcaaa acgtgctgaa atcgataaag caaaacacga attagaagat
     1681 gcagaaaata actatgattt agaacgcgct gctgtattgc gtcatggtac gatacctcaa
     1741 ttagaaaaag aattgaagga attagaagct aaagcaaaag acagcgagat taagatggtt
     1801 caagagtccg ttaccgaaaa tgagattgct caagtcgttg gcagattaac gggtatcccc
     1861 gtaacgaaac ttgtagaagg tgaacgagaa aaactaatta aattgaatga aacacttcat
     1921 aaacgagtga ttggtcaaga tgaagcagtt gatgcggtca gtgacgcggt aattcgttca
     1981 cgggctggat tgcaagaccc aaatcgtcct ctaggttctt tccttttcct aggaccaact
     2041 ggtgtcggaa aaaccgaatt agcgaaagcg ttagctgaga acttgtttga ctcagaagat
     2101 catatggtac ggatcgatat gagtgaatat atggaaaaac atgctgtgtc tcgtctagtc
     2161 ggagctcctc caggctatgt aggttacgaa gaaggtggtc aattaacgga agctgtcaga
     2221 cgaaatcctt atacaatcgt cttgttggat gaaatcgaaa aagcgcatcc cgatgtcttc
     2281 aatatcttgt tacaagtcct agatgacgga cgcttgactg attcaaaagg gcgagtagta
     2341 gatttcaaaa atacggttct gatcatgacc agcaatatcg gttcacagtt actgctggaa
     2401 ggcgtgactg ctgatggaac gattccagaa gcagtggctg aacaagtcaa cacgctatta
     2461 cgtggaaact tcaaaccaga attcttgaac cgaatcgacg acacgatttt atttacacca
     2521 ttgagcctag acaacgtgaa aggaatcgtt gacaaaatgg tggcgcaatt ggcacaacgt
     2581 ctggaacatc aagaaatcct gctgacaatc agtgatgaag ccaaaacgtg gattgctgaa
     2641 aatgcttatg aaccagcata tggtgcaaga ccattgaaac gatttatcac taaggaagta
     2701 gaaacaccac tagctaaaga aatcgttgca ggacatgtta tgcctaaatc aaaagtaacg
     2761 attactttac ttgatggaca acttcatttc aaaacagaag agctagaaga aatcgtctaa
     2821 tcttccattg agactataaa agaaaaaagg ggtctgacac aactagtgtc agacctcctt
     2881 ttttcaaata aacagtgttc cagaagtagt ttcttcgaaa aataagccga aatttcttga
     2941 agctgcccgc ttctgtcaca acctctttta ttaggtattt ttcacttttt ctaatttata
     3001 actttcaaag taacgctcat cttttttact ttgagaccat gcaaacaatc ccattaaggc
     3061 agcacctatc gtatttgtca gcaagtcgcc catcgtatcc atcaatgctg ctcgtccgac
     3121 aaacagtgtg ccatcagaag cagcaaaacg ttgaagattc atgcctaaga actggtcgca
     3181 taagaattcc cagaactccc aaaacacgcc gcaaagtcca gcaaacgcaa agccgaataa
     3241 gaggaacaac caaggactgg tttttgatat ctcagcatct ttcatcaagt aaccaatcaa
     3301 tccatagcca agagctgtca ggaccattgg actcaccgca tgtaagattt tgtcccaaaa
     3361 agagatgata ctgatcaaat gcatccctgt gcctaaaaag acagagatga acaagaaaaa
     3421 ccagtaaaac aaaacgattg ctgctggtat tttaagtttg aaaactttca aaatgacttg
     3481 tggcatgaaa atcaatgcaa tccctaataa agattccacg ataaatgtga ttccttgaga
     3541 agtcgaatat ttttgtgtga taaattcata aaaattatag cagacaacga atacaccaaa
     3601 agcaagtaaa gctatttgca cctttttgat ttctttaatc actgaaagtc cctccatcta
     3661 aaaatctttt tacttctgaa agcaactcaa tttctgaaga aaccactttt ccgttcaatt
     3721 tgatcaaacc tattgtatat aagttcaggt agtgaaattg attttcagct gtttcttgta
     3781 gtgcagcgat ttttttcggg tgggttccgc cttgctgtcg agtatcagta tataagccga
     3841 tcactgggat gcctttcgca taagccacac cgatttccga tgctaccccg gcatcgatcg
     3901 tcagcccatc tagtaaagca atcattaaat cactagccaa cactttttct gtgtcagcaa
     3961 gagcaatcat tcgactgtct gcataagcag ttttgtcatt gattgcttca ttttcttgag
     4021 gaagatagat cgaaagctca gaagagagct ctctgatttc tttaactaac tgttggttat
     4081 ataacaaatc acttttagca aacattggtg cggcaaaata aattttcatc taaacatcat
     4141 cctttttatc aaaaataatc gctaaagtaa gcttccatgt ttggaatgat acgctctagt
     4201 gacttcagtg tttcttcatc tgtttctaac cgttcgattc ttgccaaata agaagcacgg
     4261 cgataattca tcatcaagca agtcaatgtt tggatatcca gtcgaacgga ttttcctata
     4321 ggttcattta agacacggac ttgtccctgt tcatcccatg ttaaagcgaa gacaccatta
     4381 ttccaaggag cgacaggatc ttcaatgatg aaatgaaaag catcagctgt tccaacaaac
     4441 ggaaaccgtt gaaggaattc ttttacatca acgatccgag ccataaaata tggttcgatc
     4501 tgctctttga tctgactatc ttcaatcaaa aatgctaaag gctcattttt ataaatatct
     4561 ccatgtaccc agtagatcat ggaaaaatgt gcggaaataa agttccataa gccatttcga
     4621 gcttcttgat caagatagaa catttctttt acgtgaaata tttcttcagc tacccagtag
     4681 aacagaacac ctcttggttc atggttgcta tcgtagtaga cagcggctgt gcgctcatct
     4741 tcattttcga aacgccaata ctcttcccaa tgaaaatcac tgcggaataa agcaccatgg
     4801 ttttgttggg caaatttgtc ataaacggaa aatacgtctg gatgatcaac tggtaatcgc
     4861 tcaaccatac cacttactgg gatttgttta ggtagctgtg tatctctgat tttaaaggaa
     4921 agtttatcag acatgatttc ccatccttta cgccgatagt aggggatact gtaaggatac
     4981 aaataagaga tccattgctt attcttgcgc atattatcca atgcaacgat aattagatct
     5041 tgcatcaatc catggttagc atattctgga taagttccaa ctccagtcac accacccatt
     5101 ttataacgtg caccgtgaat attgacttca caaggataga tagcgatttg agagatcagt
     5161 ttattttcgt ggaaccatcc gaacactttc gatacttcca aaatcggttt tttggaacgg
     5221 ataaaagctc gtttattctc aaacccgctt tcctcgatgt ctgcttcagt cacttggaac
     5281 acataggaaa gcaattcatt gaattgatcg atatgttctt gagtgaccgg ttttaattca
     5341 agattttctc taaaatcttt ttgatccatt ttttcatctc catttattag cttgataaca
     5401 gtatagcaaa attctacaaa aaaaacttct atagctgact aattaaaaac tctttaaatt
     5461 actgcttttc gttggtataa tggaaagtgc tgattatatg aaaagaggag acgctatgga
     5521 tataaagaaa atgaaagaac gccaagagca gattcgtaat ttttcgatca tcgctcatat
     5581 cgaccatggg aaatctacat tggccgatcg tattttagaa atgacgcaca ctgtgacgtc
     5641 tcgcgaaatg caagatcagc tgttagactc gatggatttg gaacgtgaac gtggaatcac
     5701 gattaaatta aatgctgtag aactcaatta cacagcgaaa aatggagaaa catatatctt
     5761 tcacttgatt gacaccccag ggcacgtcga cttcacctat gaagtctctc gaagccttgc
     5821 tgcctgtgag ggtgccgttt tagtggtcga tgcagcacaa ggaatcgaag cacaaacatt
     5881 ggccaatgtt tatttagcgt tagacaatga tttggagatc ctgccagtca tcaataaaat
     5941 cgacctaccc gcagcagatc ccgaacgtgt ccgtcaagaa atcgaagacg taatcggaat
     6001 cgatgctagt gaagctgtat tagccagtgc caaatcaggg attgggattg aagatattct
     6061 cgagcagatc gtggaatatg ttcctgcacc aagtggagac ttagatgcac cgctaaaagc
     6121 tttgatcttt gactctattt atgatagtta ccgcggtgtt gttctaaatg tccggatcac
     6181 ggacggtatg gtcaaacccg gagacaaaat caaactgatg agtaatggca aaacatttga
     6241 tgttacagag gtcggggtct tctcgccaaa agcagttgct agagactttt tgatggtcgg
     6301 tgatgtcggt tatatcacag caagtatcaa aactgtccaa gatacacgag taggggatac
     6361 agtaacgtta gcagacaatc ctgccgctga agctttacca ggttaccgaa aaatgaatcc
     6421 gatggtttat tgtggtcttt atccaattga tacttctcgt tacaacgatt tgcgggaagc
     6481 tttagaaaaa ctgcaattga atgatgctgc tttacaattc gaaccagaaa cctctcaagc
     6541 attaggtttt gggttccgct gtggattcct cggacttctt catatggatg tcgtccaaga
     6601 acgcttggaa cgggaattca atctagaatt gatcacgact gcaccatccg ttatttacca
     6661 tgttaataaa acggatggta cgactgctac agtagataac ccggcagatt ttcctgaacc
     6721 tgtaacgatt caggatgtag aagaaccatt cgtcaaagca cagatcatgg tcccaaatga
     6781 ttttgtcggt gcagtaatgg aactttctca acgaaaacgg ggagaattca ttacgatgga
     6841 ttatttagat gattaccgag taaatgtcgt ctataatatt ccgctttctg aaattgtctt
     6901 tgactttttc gacaaattga aatcaagcac gaaaggctat gcctcattgg attatgaaat
     6961 gtctggttat caaaaaagca agttagtgaa gatggacatc ttgctgaatg gagaaaaagt
     7021 cgatgcatta agctttatcg ttcaccgaga ctttgcctat gaacgtggaa aagcaatcgt
     7081 tgaaaaactg aaaaaactaa tcccacgcca acaatttgag gtgccaatcc aagcagctat
     7141 tggacaaaaa atcgttgccc gttcagacat caaagccttg cgtaaaaacg tcttggctaa
     7201 atgttatggt ggagatgttt ctcgtaaacg taaactctta gagaaacaaa aagaagggaa
     7261 gaagcggatg aaacaaatcg gctccgtcga agttccgcaa gaagccttca tggccgttct
     7321 gaaaatggac gaggacgaac cgaagaaata gcaagggttt gtttgaaaac agtgtaaaaa
     7381 ataaaccatg tttacatgga tttgccaccg gtttgccacc ggtggttttt ttcggtggca
     7441 aaaatcaaga attctttcta agattttcca tgatatcgac cgtttcattt ctcatcttat
     7501 ctgttacatg cgagtaagta tccatcgtaa ttgaaattct gctgtggccc aatcgttcag
     7561 agatttcttt catttttgcg ccattttcga gaagaagtgt agcatgagta tgtctgagag
     7621 aatggaagtt aaaagagagg gagagtgcat tcgatatcct cctagtattc catttcacta
     7681 cacttggcgt aactagctca ccgtcttcct tcgtacatac tgcatttgaa tcaatgtaga
     7741 gctttccata cttcattcga ttttctaatt gttgtttctt atgttttttc agaattgcta
     7801 gcaaggtttg tccaataaaa atcgttcgat tggaactgct tgtctttggc gtaccatata
     7861 cccatgcgcc gtcattcttt accatttgtt tctctacagt aattgttcca tttgaaaaat
     7921 cgacatcatc ccacgtcaga ccacaaactt cgccaacgcg cattcccgta taaaatccaa
     7981 tattcaaagg aatatagaaa ggatgtcctt caggagtaat ttctagcata tgatcaaagt
     8041 cctcaagaga aatgattttt agatcttttt tagtcgttgg tcgtgcttca tattttggta
     8101 tttttacata cagcatagga ttttgcttga ttaatcccca aggataaacc gccatattca
     8161 gcgcattctt aaggacagag tgagtaatag tcattgtttt cttcgagtaa ccctttttaa
     8221 attcagcatt gatgaaattt tgtaaaagag caggggagag atccgtaagt tttttctttc
     8281 ctaaataacc gtttatatga tttttgatgg taaatcggta gttttcatag gtattgtatt
     8341 ttagatttag tttaacgtat tcctccatcc aaaaatcaag gtattgtttt actcgagtat
     8401 ccgtacctaa aaagtattgt cctgtttcgt caatatctga taaaactttt cgtaaagcag
     8461 cttcggcctc tggtcgggtg tctccgccaa ctttctccac tttttttctt gagccatcat
     8521 cattgatatc t
//
LOCUS       DAKBVG010000117         8400 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000117 DAKBVG010000000
VERSION     DAKBVG010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.117"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            173..2875
                     /locus_tag="OUD09_001842"
     CDS             173..2875
                     /locus_tag="OUD09_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCT9693105.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            3027..3344
                     /locus_tag="OUD09_001843"
     CDS             3027..3344
                     /locus_tag="OUD09_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCT9693106.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            3374..3694
                     /locus_tag="OUD09_001844"
     CDS             3374..3694
                     /locus_tag="OUD09_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCT9693107.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            3802..5262
                     /locus_tag="OUD09_001845"
     CDS             3802..5262
                     /locus_tag="OUD09_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCT9693108.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            5330..5551
                     /locus_tag="OUD09_001846"
     CDS             5330..5551
                     /locus_tag="OUD09_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693109.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            5582..5764
                     /locus_tag="OUD09_001847"
     CDS             5582..5764
                     /locus_tag="OUD09_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HCT9693110.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            5764..6177
                     /locus_tag="OUD09_001848"
     CDS             5764..6177
                     /locus_tag="OUD09_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HCT9693111.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            6300..7481
                     /locus_tag="OUD09_001849"
     CDS             6300..7481
                     /locus_tag="OUD09_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCT9693112.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(7552..7716)
                     /locus_tag="OUD09_001850"
     CDS             complement(7552..7716)
                     /locus_tag="OUD09_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693113.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            7870..7943
                     /locus_tag="OUD09_001851"
     tRNA            7870..7943
                     /locus_tag="OUD09_001851"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7904..7906,aa:Arg,seq:cct)
     gene            complement(8012..>8400)
                     /locus_tag="OUD09_001852"
     CDS             complement(8012..>8400)
                     /locus_tag="OUD09_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCT9693114.1"
                     /translation="DKHTIDLLKKFKINQKELFENFEIKNPHNFVFFNLKNGLVSSNA
                     VSKYLRKKLKELGLEKQFTLHGLRHTHASILLYQGVNILSVSKRLGHSSLETTMSTYL
                     HIVRELEDQDKEKINTVFDSLYKNDN"
BASE COUNT         2722 a         1419 c         1754 g         2505 t
ORIGIN      
        1 aaaggatttc agggtgtctt ttctatattt ttttatacaa aaaagatgtt ggtgaagttc
       61 accaacatca aaaagtatag agccgaaata aaggagcaag gcgcaaataa gcgccttttt
      121 atgctatgat tatgaaaacg ggaacaaaat tggttgccag aggaggggtt atgtgaaaga
      181 acgtcaagaa gagatcttgc agctattgac agaaaaccaa caaggattga cagcaagtga
      241 tgtagctgag cgcttaacta tcgatcgaag caatgcaagc cgatatttaa gtgaactata
      301 caaggcccat catattgtaa aaaccgctgg ccgaccagtc gtttactctt tgccaacgga
      361 aaagtcaaaa tctgatgaag tgcatgtcga ctcttctacg caagtgactt ttgaaacact
      421 cgttggagaa aatgattctt taaaagtcag catacagcag gcaaaagctg cgattcttta
      481 tccaccgcgg gggttgcata cgattatttt tggagaaact ggtacaggga agtcgatgtt
      541 tgctgaatgt atgtatcatt ttgcaatcga ttcagagatg ctttctgcag atgcgccatt
      601 tgtttctttc aattgtgcgg attatgctca aaatccgcaa ctgctctttg gacacatctt
      661 tgggatcaaa aaaggggctt acacaggagc agctcaagat agtcccggtc taatagccaa
      721 agcagacggc ggtatattgt ttttagatga gatccaccgt ttaccgccgg aagggcagga
      781 aatgctgttt actttcattg ataaaggtat ttaccgtccg cttggagaaa gtgcacaagt
      841 acatgaagca tctgttcaga tcattggggc gacgactgaa tcttcagaaa gtttcttatc
      901 tacgttcaat cgacggattc ctatggcaat caccttgccg aatcttgctt cacgttcact
      961 ggatgagcgt tatgaaatca tctctctttt tatcaaacaa gaagcgaatc gtttgaatca
     1021 acgtatcgat atcgaaaaag aagcgattct tgccttcatg ctttatgatg cggaaggaaa
     1081 tatcggtcaa gtgaaacgag acttgaaact agtctgtgca aaatcttttt tacattaccg
     1141 tactcatcat gagaaaaagc taatcattcg aaaagaagaa ctttcccttc aagtacaaaa
     1201 aggattactg aaaatcaagg aaataccgga acgtctcgac cgattcatgg acagcaaaag
     1261 tcagtatttg acatttgaac caggatttgc tgatgttgtc tggtcacaag atccagaacg
     1321 aaacatgcag gtctataacg atattgagga aaaagtcact tctctttccg aaaccggtgt
     1381 ggagaacatt gatctagaga ccctcatttc aaaagatgtg gatgcttatt tccagacata
     1441 tgtcaaagaa ttgactaaaa gttcgattcc caaagatctt ttgccagatg aaatctggcg
     1501 gttaaccaat caattgtatg atgtggctga aaaacggtta gaccgtattt ataatgaaaa
     1561 agctcgtttt gcttttgctt tgcatctgca aagtacattg gatcgtgtga gagaaggtca
     1621 tatgatcgtc cacccagatt tgaataacgt acggaaaaat tttaagaggg aattccaggt
     1681 agcaatcgat ctttcaacga tcattgaaga agaagaacaa gttgagattc cttttgatga
     1741 aatcggcttt attagtatgt ttctttctat caatctagga gaaagtgagc ctgttaaaga
     1801 aaacaaagta gatgttctca tattgatgca cggcagacag actgcttcaa gcatgttgga
     1861 gatggcacaa gaactattag gaacaaaagt agggaaagct tttaatatgc cactggttat
     1921 ggaagttcaa acaatgtatg aagaagtgtt acaatatgtc aaaaactatc aagagcattt
     1981 gacgaatggc ctactcttgt taacagatat gggatcatta aacaccttca gtgatttgat
     2041 ttatgaagag actggcattc ggacgaaagc tatcacgatg acaagtacca tgattgtgat
     2101 agaagcgata cggatggcaa gtgttggaag aagcttagaa gatatttatc aaaatatcca
     2161 gctttccttt gaatcgatcg tacgtgatca gttcaaagcg gaaaagtctg aaactttaca
     2221 gcgtaaaaaa gccgtaatcg tgacatgctt tactggtgaa ggagtagctg ccaaattata
     2281 tcagcgtatt atgccagtga ttaatcaaaa taaggtagaa ctgatccaga tgcagttttt
     2341 agaacgtgaa tcattcaaaa aacacatcga tgccttattg gaagaatatg agatcaaagc
     2401 aattgcggga acagttgatg ttgattatca aaatatccca tttttttcag cctatgacgt
     2461 ttttgatgat gaaaaactaa atattttaaa gcgtatagct gatgatgaga tcccgacagc
     2521 taaaattgtc gattcacttg ctggatcatt aagcgatgtc ttttctattg atgaactgat
     2581 ccgttcattg caaaagacag ttcagcagat ccagaccgat ttgtcgatta tcgtggaacc
     2641 tggagtagat actggaatcg ttatccattt agccttcatg ttagacggat tgatcaaaca
     2701 agaaagccat cggcaatttg agaaactgtc tattttctca aaaatttatc gtttggaaat
     2761 ggatatcgtt cgggcaaact tgatggcact agaacgaaaa tatcaggtaa aaattccaga
     2821 agatgatatc gcatttctta cacaaatgtt tcttgaaaat aaaataaaaa aatgaacaca
     2881 ctaatcatac tgtttacaca ctaaacatct gtgtgcgcac tataaaaaag agctaaaaag
     2941 catgaaaacg ctatttcagc tctttttttt attggcatgc ttcttgcgtt atataaaagt
     3001 gtaaaaaaat aaaaggagtg ttaaaaatgg caaaaaaaac aatcatgtta gtttgttcag
     3061 cagggatgag tacgagttta ttggtcacaa aaatgcaaaa agcagcagaa gaaaaaggga
     3121 tggaagccga tatttttgcg gtctctgcct cagatgcaga taataattta gaatctaaga
     3181 atgtcgatgt attattatta ggtcctcaag tacgtttcat gaaagctcaa tttgaacaaa
     3241 aacttgcgcc taaaggtatt ccattagatg tcatcaatat gcaagactat ggcatgatga
     3301 acggagaaaa agttcttgca caagcagaaa atctaatgaa ataaatcaag tatgcagttg
     3361 ggggtataac aacgtggaag atcaacaaaa tctagaagcc atcatggggt taatcatgtt
     3421 tggcggtaat gcaaagagcg atgcaatgga agcgattgcg gctgctaaaa tcggtgattt
     3481 tgagttagcg gataggaaaa tcgctgatgc agaagaatca cttatacaag cgcatcatgc
     3541 acaaacagac atgttgacac aagaagcaca aggaaatcat atgcaagtga cattattgac
     3601 tgttcacagt caagatcact taatgacatc tattgcgttt actgatttag caaaagaaat
     3661 catcgaactt tatcgtcgcg tatacagcga ataaatacaa cttctttttt gaacggaaga
     3721 agaaaaattt ttgaacggat tttttatttt gttataagga gaactctctt tatataaaaa
     3781 acttatagga ggaagtaaaa aatgaacgga ctatcaagtt ggatggagaa atacatcctg
     3841 ccggttgccg caaaaatcgg agcgcaaaaa catttagttg ctttgcgtga tgcttttatc
     3901 ggcacactgc ccgcaacaat ggcgggatca gtcgctgtaa tgatcaatgc aattattcgt
     3961 gatttaccgc aacagtttaa cagtacctat gctgacagtt tagcagcaga caaaggaatc
     4021 tttgccgtat tgaatgtcat catcggaatc aatggttttg tctggaacgg tacactagca
     4081 atcgccggat tgatctttgc cttttcttgg gggtataatc ttgcccgagc ttatggagta
     4141 aatgatctag ctggggggat cgttggttta gctaccttga ttcaaggtat ttcctttggt
     4201 ccagcattat cagcagcatt agatgtgaat gttccttcaa atgtcgcaga tgcaatcaat
     4261 agttctgaga ttggtgcaac gattgcagat ggcacactgt cagttggatt atggggttgg
     4321 ctaaaactag atcatttgaa tggaaacgct tattttacag ttatgatcat gggagctatt
     4381 tcagtcatta ttttttgtaa attgatgctt gccaacatta cgatcaaaat gcctgattca
     4441 gtaccaccag cagtatctaa agcatttgca gccattttac cagcgacgat cgctttatac
     4501 gtcattgcta ttatcaatta tatcgtaggt cgtgtagctg atgggcaact gatcattgat
     4561 ttggtgcaaa aatatattgc agaaccattc ctaggattat ctcaagggtt gggagcagta
     4621 ttgatcgtca cagtatttgt tcaagtattc tggttctttg gtattcatgg atcaaatgta
     4681 ttggcaccag tgctagaggg aatctgggga caagcacaac taatcaatat tgatattttc
     4741 caaaaaggtt ataacggaaa aactggtaca ccagccgtgt tagatgcaat cgaagatgga
     4801 aaagcttata tgtgggtccg cggatcgttc gatgcttttg catggtttgg cggatcaggc
     4861 ggaacgatcg tcttactgat agctatcttg attttctcta aacgtgcaga ttatctaaca
     4921 gtagctaaat tatccattgg gcctggtatc ttcaatatca acgaaccagt gatgtttggt
     4981 ctgccaatcg tattgaatgc aatcatgttt atcccattct tgattgctcc agtagtagca
     5041 acagcaattg gatggacagc tacttatctt ggcctagtcg ctccggtatc gcagcaagtc
     5101 acatgggtag taccgccgtt cttattgtca ttcttagcaa caggagcgga ttggcgggca
     5161 ccgatcgtaa cattagtatg tatggtcgtg actttcttga tttgggcacc atttgtcatc
     5221 tctgcaaaca aaatggaccc tgccgcaggc gaacaacaat aagatgaacg tgaggagcgg
     5281 gacgctttaa gtttcgttcc tcataatgaa ttttcgagga ggaactgaaa tgattacaat
     5341 tgccagtgca atgcaacaag atgaaatcaa agaattgtta gaaggataca atgaagaagg
     5401 aatgactttt acatttaaag aaaaacaagg aatcaagctg ttctttgaaa caagtgcaga
     5461 agatccggaa gttgcggcta aaaaagcaaa ggaactaatc aaagcacagc cttggggaac
     5521 agtcctttat tttcaatcaa cagctgttta gaaaatggtt taaaaagata ggatggttga
     5581 gatgataaaa aacggtttat ttttatgtag tattggttct ttgatcgtgt tgtatagttt
     5641 gaatccacat tcgcaaaggt atgatttgta tagtatggtc acaggtcttt ttctaacagg
     5701 attaggaatc tatttcttca tgaaggggaa aaaaagagaa gaagcaaagg agaaaaataa
     5761 ctaatggaaa tcacgaaaaa attaaatatt ccagcatcgt ttttttacga taaagttatg
     5821 gactctgtcc ttttcgatgt acgtaaagcg accggcaaat cagtaactag aaaacagctg
     5881 aaaaatttgg agtacgtcaa acaattctcg caaaataacc gggctcgcat aaaaatcgaa
     5941 gaagtcattg aaaaccagtc ttataagttt cgtacatcga caacgaagaa tgaattcgtc
     6001 gttcattatc aaatcaaacc attggacgat tcgacctgtg aggttcatta cacagaaaaa
     6061 atggagtctt atggcatgct tcaaaagttg aatgatatgc ttttaggaac aatcttgatg
     6121 tattttaaac ggagacgttt caaaaaaatg ctagatatga tcgaagcaac gtattaaatc
     6181 aatgaaacat ggtactattt agttattgaa aaatgaagta agctagaagg aatttttcat
     6241 aggaagatag atctttcctg cgaatcctca ctagcttttt tgtaggaagg ggcagcaaaa
     6301 tgacaaacaa agtaagagag ctaggtgtat cttcgattga ggaaatgttt gatttagcca
     6361 cttatgcatt taatggggca gatacacctg aaaggcgaga acgttttcgt acacttgcgg
     6421 agaattcttg gaattacgga ttttttgatg aggaggatcg gctaacaagt caagtcatgg
     6481 cgaccccttt tccagtgaat ttttacggac aagagtacct aatggccggc attggctatg
     6541 ttgcttctta tccagaagca aggggacaag gcgggatcaa tcagatcatg gaaaagatcc
     6601 ttgaagattg ccgtgatcgt aaagtaagct tatcttatct ggcgccattc tcctatccct
     6661 tctatcggcg ctatggctat gaacaatcat ttgataaaat cagttatcaa ctaaacagtc
     6721 gtgatgtgcc gtttatcaaa aaaaaatctg gaaaaataaa gcgtatggat tttgaagatg
     6781 caaaagctgc aatcagaaga atttatgaac agatgccgga aaaccaacga gctggtctga
     6841 agcgcgaaga ctggtggtat acgtataaat tcaagcaaaa aggcaattac cagtttgctt
     6901 tgcattttga tgataatgag gaagcaacgg gatatatcgt ttatcagctt gcaacgtctt
     6961 cctttattat tgcggagtgg ggatttttgg atcatgatgc tttttgcagc cttgttcgct
     7021 tcgtaagttc tcacaatggt gcttttgaaa catttgaata ttcatgcggc tacggcggta
     7081 atgatcaaaa ttatctatta gaaaatccat ttgcttctgt aatgatcaca ccttatatga
     7141 tggcgaggat cgtagatatt tctttgtttt tggaaaatta tccatttaaa aagagacaag
     7201 ctgcatttgc cctacaaata gaagaagatc attatgcgaa atggaatgaa ggaatcgtgg
     7261 aagtaacaat cgaaaatgga aaaaatcatc tctcaaaagt agaacaaaca aaactcccaa
     7321 tcgttcgagg aagtatccaa catatcacgc agctattatt agggtatcgt aaagtggaag
     7381 agttggtatt ccaagaaaga atacaggtga caaaagaata tgaagaaacg ttaagctcaa
     7441 tcttgccaca gcaacagcca attcttaatg actatttttg ataaaaaacg ggtgatgaca
     7501 atatttttgt caccaccctt ttgtttttca tttggcattt gaaggaaaat gttaaagtaa
     7561 atcctctttt ttcgtaaatt tgaatttttt gttttctttt gttttagggt tttctttttt
     7621 gtggtcttgg attgcttctt tcgtaatctt ttcccgattt tttttagatg aatctaagcg
     7681 ttctgcgtat actttttctt ttggcttatc attcattgga aaggacctcc ctacattatt
     7741 tcattctacc tctagtttat catctgttaa ggactagtgg aaacgttttg cctaacttat
     7801 aagctgattt ttttagcaga taagcttgca agtaaaaaac aaacaagcta caattgattt
     7861 attgaaattg ttcctgtagc tcagtaggat agagcaatcg cctcctaagc gataggtcgc
     7921 tggttcaatt ccagtcagga acgtataaat aacccctgta tcctttgcgg tacaggggtt
     7981 tgttttgtat ttgtgccaaa aatgtgccaa actagttatc atttttatat aaactatcga
     8041 acacagtatt gattttttct ttatcctgat cttcaagctc tcgaacaata tgaagataag
     8101 tagacattgt agtttctaaa ctgctatgtc ctaaacgttt cgatacgcta agtatattta
     8161 ctccttgata aagtaaaata gatgcatgtg tatgccttag tccatgcaaa gtaaattgct
     8221 tttcaagccc taattctttt aattttttgc gcaaatattt gctgacggcg tttgatgaaa
     8281 ctaatccatt ttttaagttg aaaaaaacaa aattatgagg gtttttgatt tcaaaattct
     8341 cgaataattc tttttgattg attttaaact tttttaagag atcaatcgta tgtttatcaa
//
LOCUS       DAKBVG010000118         8304 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000118 DAKBVG010000000
VERSION     DAKBVG010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.118"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(21..251)
                     /locus_tag="OUD09_001853"
     CDS             complement(21..251)
                     /locus_tag="OUD09_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693115.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            complement(687..1253)
                     /locus_tag="OUD09_001854"
     CDS             complement(687..1253)
                     /locus_tag="OUD09_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCT9693116.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1253..2209)
                     /locus_tag="OUD09_001855"
     CDS             complement(1253..2209)
                     /locus_tag="OUD09_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCT9693117.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2209..2865)
                     /locus_tag="OUD09_001856"
     CDS             complement(2209..2865)
                     /locus_tag="OUD09_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCT9693118.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3235..5253
                     /locus_tag="OUD09_001857"
     CDS             3235..5253
                     /locus_tag="OUD09_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCT9693119.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    5323..5529
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5680..8094
                     /locus_tag="OUD09_001858"
     CDS             5680..8094
                     /locus_tag="OUD09_001858"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCT9693120.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
BASE COUNT         2751 a         1563 c         1651 g         2339 t
ORIGIN      
        1 ctcattaaaa agcataacca ttactccgat ctgtttttaa aattagtttc tacctctaat
       61 atatatcgag tttttatttt gccttcagag aatactgttt ctttttttgc agttacaggt
      121 tgtttatttt cggaaaaagc taatatagct aagattgaaa catccatctg gaatttatct
      181 tttttactgc tttgatcata aaagtctgca tattcatcac tgatattttt tctaataaat
      241 tcttccatca taaaaatcac ctcaaaacga ttatacgaac ttacgttctt gttgtaaagc
      301 gatatttgag gggcaaaaaa ggggcataat ttgactgata ttaagttagc tattgatata
      361 tgaatgacat acaaaaatat aaaatctttt aaaaatgctt atatatcaat gtttttgcca
      421 ttcttttata tatctgttat atgtcaaaaa aatgtgttca atttcaattt gtatttgaat
      481 aaagaggttg tgaaaaagct gtcctgcatc aagtaataag aacagccaat caaaaacagc
      541 ttctcatgtt tttgatttgg ttggacttat taccgcagat ataagctttt gagcaccgtg
      601 aataaagagg ttatgaaatc aacgctctaa cctgagtatt agagaaaaaa acgcaaaaca
      661 tatcatttat gttttgcgtt ttttatttat ctttttttct caatacaata taaaataggc
      721 ggctggttct tttggttgat aaaccgataa ttcagcacat tatatgtttc ctgtggcagt
      781 ttggaacaaa aagaatcaac catatccagt tctttttctc caccttcatg accatagtag
      841 acgacaagga tcaatcgtcc gcgaggcaca agtcgtttca atatttcttc catagctgtg
      901 cgtgtagtct ctggcatagt gatgattgct ttatcacttt ttgggagata ccctagattg
      961 aaaatccctg ctttgagatt ttgttgttca gctatcactt ctcctaacgt ttcgtggcct
     1021 tgtagaaaca atgagacacg ttcttctaac tgacgctgtt ctagcctttc acgagtagca
     1081 tcgattgctt gttgctggat atcaaaacta tagacatgac cagtttttcc gatgtgttct
     1141 gctagaaaaa ccgtatcgtg gccattgccc atcgttgcat ctacgacgtg atcccctggt
     1201 tgcaagattt cttgcagtaa tgtatggctg aaatgcaaag cagtttgcaa catcagacgc
     1261 tcaccttttt ccgtacatca tattttcctt ggaagctatc tctgcgaaga agttcttcat
     1321 caattgcatt caagacttcc cattttttca agctccacat tggtccaatc aaggaatccc
     1381 acggcgcatc ccctgtcaaa cgatggatga tgatctcttt tggtatcatt tccagctggt
     1441 cgcaaatgac attgacatag tcaggacggc tcattaattg aaggcgacct tcatgatagt
     1501 cccgcaacat tctcgttttg cgcatcagat gcatcaaatg gagcttgatt ccttggatat
     1561 ctgaatctaa aatggtgcgt ctgacatttt ctttcatcat ttctaaactt tcgcctggta
     1621 atccgttgat caaatgcgta caaacacgga tattatgttt gcgtaattta gccacgccat
     1681 ctaaataagt ttgataatca tgtgctcggt tgatcagttt tgaagtttct tcaaaagttg
     1741 tttgcaatcc aagttctacc cataaataca tccgctcgtt cagttcagca aggtaatcaa
     1801 ctacttcatc tggcagacaa tccggtcgtg taccgactga taatcctacg acacctggta
     1861 aattgactac ttgctcaaat cgttctttga ttatttcaac tggcgcatgg gtgtttgtaa
     1921 aattttggaa ataaacgatg tattgttgga catgaggcca tttttgatgc atcatgtcga
     1981 tttctttctg gaattggatt ggaagagggt cttcaggtgc aacgatcatg tctcccgaac
     2041 cagatacact gcaaaaagta caaccgccat gggcaacagt cccatctcga ttcggacagt
     2101 caaatcctcc atcgatcggc accttgaaaa ttttcccgcc aaactcattt cgtaaagcgt
     2161 aattccaaga atgatatctt ttatttcctt ctgaataagg aaaagtcatt aaaagtcctt
     2221 ctttctgtaa gagttatcta tttgttttct tttttctcta tacactggat aagtcaggca
     2281 cagccaagaa agactgagta agtagccgcc gatgatatca gtaggaaaat gaacgcccag
     2341 ataaatgcgg cttatcccga cacttactat aataaacatc atcagcaatt gaagcaatgt
     2401 gcgtaaggtt cttttttgaa caaaagctgg aagcagaaat agtaaagaac cgtaaagaat
     2461 catactgcct gctgcatgtc cactagggaa actgtaactt gtttcaacta ctaagtgcgc
     2521 caatgaagga cgctctcgca tgaaaaatag tttgatcacc gggttgatga ttccagcaat
     2581 tacaataaat ccagaaacga accaaaatgt ttctgccctt tttttcttat accacatcca
     2641 taaagccgca caagcaaata aaatgatgac ggtaagagga ttaccgaatt tagtcataaa
     2701 gagttggtag ctgttcagat caggataaga agctctgaca atgcctgtaa accactgatc
     2761 gaaaccagaa agccacgaca gatggaactt tacgatccag cccaaaatga aaaacaggac
     2821 aagaaagaag ctaccagcca cctgaaatga tgttttatat ttcatacaat tcctcctttg
     2881 aagcaatgac ttattatagc atttttggtc cagcgaaaaa aacggtaaag agataaaacc
     2941 aatgtatgat tcctctttga ataaccaata ttcttataaa aaaaataact gtaatcggaa
     3001 agacattttt tggtaatgca aatgaaatgt tagggaaata aaatcagtgt ttttcgaaat
     3061 ccttttaaac aagaacgtag acgtaggaag aacttgaaaa tgaacagaag ttgttcatta
     3121 cggtaaatta caatatgatt acaaaaaaca acaaagctct taaataactt ttaataaata
     3181 ttaagagaag ttataattat taataataga taaattgaag ttggaggata gaagatggaa
     3241 aacattgcaa gaaaagaacg gagacgttta gaacagacca aacgttttcg aaaagcaaaa
     3301 cgaggggcag caatcgtggg aactgcgatg gtaggctgtt ctgtcgctgc cccattagta
     3361 cagccggttc ctgttcaagc ggatgaaaca cctgttcagt tcggcgcaag aatcaataca
     3421 gcggcattta tcacagaaat tgctacttat gctcaaccaa tcgctcaagc caacgatcta
     3481 tatgcatcag tgatgatcgc acaagcagtt gtagaaagcg gttggggcag tagtgcgtta
     3541 tctcaagcac cttattataa tctttttggg ataaaaggca gctatcaagg acaaaccgtc
     3601 tacatggata cgcttgaatt tttaaataat caatgggtaa ataaaaagga gcctttccga
     3661 caatatcctt cttttgctga atctttcaat gacaatgcct atgtacttag aaatacttca
     3721 tttggcaacg gttattacta tgccggtact tggaagagca acacgaaatc ctatacagat
     3781 gcgacagctt gtttgacagg gcgttatgcg actgatcctg gttacgcagg caaattaaat
     3841 aatatcatcg caacttacgg attgacccaa tatgatacac cagctagcgg aaacgcaggc
     3901 ggtggagcga ctacaggtgg caataatact ggaagtgcag gtagtactgg cggttcaagc
     3961 aattctggta atacaaactc aggttcagca acttcgacca cttatactgt ccaatctggt
     4021 gattctgttt ggggaatctc aaataaattc ggtatcacaa tggatcaatt gatccaatgg
     4081 aacaacatcc aaaacaattt catttatcca ggacaaaaac tgacaatcaa aggtagccag
     4141 gaaaatggct cttcgacgaa taattccggt aacaatacca actcttcagg aaatgctggt
     4201 acatcaaacg gaggacaaac aactggtgcg aaatacactg ttcaatctgg tgattctgtt
     4261 tggaagatct caaatgatca cggaattacg atggctcaat tgattgaatg gaacaatatc
     4321 aagaacaatt tcgtttatcc aggacaacag ctaatcgtat caaatggcgg ttcttctaat
     4381 gctggagcaa atactggctc aaatactggg aatacaagtg gctctagttc gtccagctca
     4441 agttcttcta atacagcttc aggaacgaaa tatacagtta aagccggaga ctctgtttgg
     4501 agcgtctcaa ataaatacgg aattacgatg gctcagctaa tccaatggaa caatatccaa
     4561 aataacttta tctacccagg acaacaactg actgtttcaa acagtggatc aacaaacagc
     4621 acgtcaggta atacaggttc gtcaaccaat acaaataaca gcagttcagt taatactggc
     4681 agcagttcgt ctcaaggcac ttataccgtg aaagcaggtg aatctgtttg gagcgtggca
     4741 aataaaaacg gcatcacgat ggatcagtta atcgaatgga ataatatcaa aaacaacttc
     4801 atttatccag gacagcaatt gatcgttaaa ggtggaactt cggcaaatac aaatacaggt
     4861 tcaactactt ctgcagcgaa accaaataca ccgaatacgt ctgctacgac aagtacatca
     4921 tccactggta atacgatgta taccgtgaaa gcgggcgagt cagtttggag cgtagcgaac
     4981 aaacatcata tcacgatgga tcagttaatc gaatggaata atatcaaaaa taatttcatc
     5041 tatccaggac agcaagtgat tgtgaagaaa ggttcatctc aatcaacaaa ccaacaatca
     5101 gttactggtt cgaaaaccta tactgtgaaa tctggagaat ctgtttggag tgttgctgac
     5161 agtcatggaa ttacaatggc tcaactaatc gaatggaaca atattaagaa caactttatt
     5221 tatccaggtc aaacgttgat tgtagctaaa taaaatagct gttcgaagaa ataacgattg
     5281 aattccacaa aaatatttgt tactataagg ctacttaaat atgacaacac ttgaaaagga
     5341 agtagtaaaa agactgaagt ttcagagagc gcatggctgg tgagaatgtg cacggatgct
     5401 tttgaactcg cctttgagtt gcccttgctg aatacccgag taagcaagcg gcggtgacgg
     5461 ccgttacaac gttcgaggcc ttttcactaa ggtaaaatta ggtggtaccg cgtagattac
     5521 gtcctataaa aagagcaatc tttttatagg atttttttat ttcttttgtg aagtttgaga
     5581 aaacaaagca aaaggaaata aaaataaaca gattctgccg gttttttcgt tatatttata
     5641 atagaaacaa gtgttttcaa tagaacagga ggacagcaaa tgagttacaa tcataaagaa
     5701 attgagaaaa aatggcaaaa atactgggca aaaacgaata ctttcaacac ccatgatgat
     5761 cctgaaaagc caaaatttta cgcactggat atgtttccct atccatccgg tcaagggcta
     5821 catgtagggc atcctgaagg ttatacggca acggatatcc tttcacgtgt gaaaagaagt
     5881 caaggataca acgtgctgca tccaatgggg tgggatgcat tcggtctgcc tgctgaacaa
     5941 tatgcgctag atacaggaaa cgatccagct gagtttaccc aaaaaaatat cgaaacattc
     6001 cgacgccaaa tcaattcttt aggatttagt tatgactgga atcgcgaagt gaatacaact
     6061 gatccggagt attataaatg gacacaatgg atctttacga agctttatga aaaaggtcta
     6121 gcttatgaag cagaagtagc ggtaaactgg gtacctgaat taggaacagt tatttcaaat
     6181 gaagaagtga tcgatgggaa aagtgaacgt ggtggctatg atgtagtcag aaaaccaatg
     6241 cgccaatgga tgctgaaaat cactgcttat gctgatcgct tattggatga tctagaatta
     6301 gttgattggc cagaaagcat taaagaaatg caaagaaatt ggatcggtcg ttcagtagga
     6361 gcaaatattg aatttaaagt ggctggtacg gataaatcct ataccgtatt tacgacacga
     6421 ccagatactc tattcggtgc aacttactcg gttcttgcac ctgaactaga tcttgttaga
     6481 gaaattacga cccctgaaca aaaagctgct gtagaagctt atatcgaaga gacagctaaa
     6541 aaatcagatc taaagagaac ggatcttgca aaagaaaaaa caggagtatt tactggtgct
     6601 tatgcaatca atccagttaa tggcaaggaa atcccaatct ggatcgccga ttatgtatta
     6661 tcttcatacg gcacaggagc catcatggct gttccagcac atgatgaacg tgactttgaa
     6721 ttcgctcaaa ctttcggact agaaattctt ccagttatcg aaggtggaga tgttgaaaaa
     6781 gctgcttaca cagaagatgg tacgcacatc aactctgaat ttctagacgg catgaacaaa
     6841 caagaagcaa tcgataaaat gaatgaatgg ctagaagaaa atggcgtagg gaaaaaagaa
     6901 gtcagctatc gcttacgtga ctggctattc tcccgtcaac gttactgggg tgaaccaatc
     6961 ccaatcatcc attgggagga cggaacagtc acagctgtcc cagaagaaga gctaccatta
     7021 cggttgccta agacaaaaaa catcaagcca agcggtactg gagaatctcc attagcaaat
     7081 atcgaggagt gggtcaatgt cgttgatcct gtaacaggta aaaaaggccg tcgcgaaacg
     7141 aatacaatgc cacaatgggc aggaagctca tggtattacc tgcgttatat cgaccctcat
     7201 aacaaaaaag aactagcaga ttatgaaaag ttgaagcgtt ggctaccagt tgacatctac
     7261 attggtggag cagaacatgc ggtacttcat ttactatatg cacgattctg gcataaattc
     7321 ttatacgata tcggtgttgt tcctacaaaa gaaccattcc aaaaactata caatcaagga
     7381 atgatcttag gcgaaaacaa cgaaaaaatg tcaaaatctc gtggtaatgt agtcaatcct
     7441 gatgatgtgg tcgaaactta cggtgcagat acactgcgca tgtacgaaat gttcatgggg
     7501 ccattagatg cttcgattgc ttggagcgaa aatggcttag aaggaagccg taaattcttg
     7561 gatcgtgtat ggcgtttgat cgttgatgag aacaataaaa tgcgtgaccg tatcactaca
     7621 ttgaatgatg gcaagcttga taaagtgtat catcaaacag tgaaaaaagt gacagaagac
     7681 tatgaaaatc ttcatttcaa tacagcaatc tcccaattga tggtctttat caatgaagcc
     7741 tataaagtgg acgctctgcc ttatgaatat atcgaaggat ttgttcaact actcgcaccg
     7801 attgctcctc acatgggtga agaactatgg gcaatccttg gaaatgatgg tggtatttct
     7861 tacgcaccat ggccaacata cgatgaagca gcacttgttg aagacgaagt agaagttgtt
     7921 ttccaagtaa acggtaaagt tcgtgcgaaa agcaatgttc cccgagactt aggaaaagat
     7981 gagctagaaa aagcagcatt agccaatgaa atagtgcaag aatatattga aggcaaaaca
     8041 gtcagaaaag ttattgcggt accaaacaag ttagtcaata tcgttgctaa ttaattggct
     8101 ctttgtcaat aaggactgat gagttgtgca aaatcaaatc tgggccagaa tgaacttcat
     8161 tctgtctcag attttttgtt atttcaaatt gattaattga ttgatcaaaa aggttttgat
     8221 tttcatgttc tcaaacacgt tcaatgtctt tttcttctga atggtcttgt aagagatctc
     8281 tgtaatcatc aaatttttgt ctat
//
LOCUS       DAKBVG010000119         8267 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000119 DAKBVG010000000
VERSION     DAKBVG010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8267
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.119"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     regulatory      119..198
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            196..1389
                     /locus_tag="OUD09_001859"
     CDS             196..1389
                     /locus_tag="OUD09_001859"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCT9693121.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1519..2991
                     /locus_tag="OUD09_001860"
     CDS             1519..2991
                     /locus_tag="OUD09_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCT9693122.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3074..3862
                     /locus_tag="OUD09_001861"
     CDS             3074..3862
                     /locus_tag="OUD09_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HCT9693123.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(3929..4597)
                     /locus_tag="OUD09_001862"
     CDS             complement(3929..4597)
                     /locus_tag="OUD09_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HCT9693124.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            4712..6424
                     /locus_tag="OUD09_001863"
     CDS             4712..6424
                     /locus_tag="OUD09_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT9693125.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6427..8199
                     /locus_tag="OUD09_001864"
     CDS             6427..8199
                     /locus_tag="OUD09_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT9693126.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2529 a         1385 c         1817 g         2536 t
ORIGIN      
        1 tctttatgga tgattgtgct aagctttttt ggacagttct aatgtaaaat atttttatat
       61 tttttaagca aaaaaatgaa aaaatacgag cagaaaactg ttttatctgc tatacttggt
      121 tcgttgcaag ttcccgaaag gatccgcagg acggagatgc cttgtaaccg aatttaacta
      181 gggggaatca ttataatggt agaaagacac ttatttacat cagaatctgt ttctgaagga
      241 catccagata aaattgctga ccaaatcagt gatgcaatct tggatgcaat tttaaaacaa
      301 gatccaacag cacgagtagc gtgtgaaacg tctgtaacaa ctggtcttgt tttagtattt
      361 ggagaaatat ctacgacggc atatgtagat attcaaaaag tcgtacggga aacaataaaa
      421 gaaattggtt atacacgtgc aaaattcgga tttgatggag atacagcagc tgtattggtt
      481 gcgattgatg aacaatctcc tgatattgct caaggagttg atgaagcact tgagatccgg
      541 gatgaagata aaaaagacgt attagatgag attggtgctg gtgaccaagg tttaatgttt
      601 gggtttgctg tagatgaaac accagaattg atgcctttac caatcgcttt gagtcatcgt
      661 ctggtacgac gcttggcaga tttgcgtaaa tcaaatgaat taacgtattt acgtccagat
      721 gcaaaatctc aagtaacggt tgaatatgat gatcaaggac aaccggaacg cgtagataca
      781 atcgttattt caacacagca tgatgatgca gtagataatg aaacaattcg tcatgatgtc
      841 atcgaaaaag tagtgaagga agttattcca gctgaattat tagatgatca aacgaaatat
      901 tatatcaatc cgactggccg atttgtcatt ggtggtcctc aaggggatgc cggattaaca
      961 ggaagaaaaa tcattgttga tacgtatggc ggttatgctc gtcatggtgg cggtgctttt
     1021 tctggtaaag atgcgacaaa agttgaccgt tctgccagct atgctgcacg ttatattgct
     1081 aaaaacattg ttgcagcagg gcttgctcgc aaagcagaag tacagctagc ttatgcgatt
     1141 ggtgttgctc aacctgtttc gatctcaatc aatactttcg gtactggaac ggttccggaa
     1201 gaagaactga ttgctgcagt gagagaaaac ttcgatcttc ggccagcagg aatcatcgag
     1261 atgcttgatt tgcgtcgtcc gatttacaaa caaacagctg cttacggtca ttttggtcgt
     1321 acagatgtag atttgccttg ggaacatacg gacaaagtag atgcgttgaa agcaagttta
     1381 gcaaaataag ttatattcag ctggaacgat cgccaagtgg tggttgttcc agcttttttt
     1441 atggcagtta acctcttgaa aaagatagta attatttatc tatttttaga tcctgtcata
     1501 atgaaaggga ggaataagat gaaaaaagaa acaaatgtta ctttggtcac agtgagtatt
     1561 ttcattgcaa cgttcatgac ggctattgaa ggtacaatcg taacaaccgc tatgccaacg
     1621 attgtcggtt cattacatgg aatggaaatc atgaactggg tattttcgat ttatttgctg
     1681 acaaacgcta tgcttactcc gatttacgga aaacttgcgg ataaagtggg acggaaacca
     1741 atctttattt ttgggacatt attgtttatt atcggttcgt tattttgcgg tttatctcaa
     1801 agcatgattg ggttgattgc tgctcgtgcg attcaaggga tgggtgctgg ggcaatgatg
     1861 ccagtagctt tgacgatcat cggcgatctt tattctattg aaaagcgagc aaaagtttta
     1921 ggtttgaata gttcagcctg ggggattgcc agtgtttttg gaccgcttgc gggaggattc
     1981 atcgttgaaa cgatcagctg gcactggatc ttttttatca atgtgccgat cggaattcta
     2041 ttaattattt tgatttggct atatttaaat gaaccaaaaa tcatacatga gtctaaacca
     2101 atggatattt ttggtagttt tagtttgatg gccatgcttg tctcattgct gttagggttc
     2161 caattattag gagacgaagg aatgagtccg cttgtttctg ctttgctgct aggtagtatc
     2221 gtaatgttag gtatttttgt ttttgccgaa aaaagagcta aagatcctgt catcatgatg
     2281 catttattca aaaatccact atttgttgtg gtcaacttgg ctgcagcatt ggctagtggt
     2341 tttttgatgg gaatcgatgt ttatatccct atgtggatgc aaggtgtatt agggctgaat
     2401 gcagcaattg gtgggctggt attagcacca ttatcactag tctggatggt cggttctttc
     2461 cttgcaagca agtggatgac tcaaatgaaa actcattggg tcttgagaat tggactacta
     2521 atcgcagcta ttgggggcgc ttggttgtat cttatgcctt tcagaagtaa ctatttatgg
     2581 ttttttgcta tatcaacggt catcggtata ggcttaggtg tgaccatgac tacctcgact
     2641 gtcagtgcac aaaatagtgt atctcgtgac cagcttggtg tagcaacttc ttttaatacg
     2701 ttagttcgaa caattggtca aacagtcatg gtttctattt ttggtttatt gataaatggg
     2761 gtaactaaac gagaacttgc agcagcaaat ttaacaaatg atgcagatat catgaataaa
     2821 ctggtgaatc cacaaacggc taaatatatt gatgcggata ttttgttacc attaagaaaa
     2881 attctttatg atgggttaca ttccgtttat ctagttggct tgttactcgt gatcattgct
     2941 ttggtcttaa cgcttttcgt tcatgaaaaa agtggtaaaa taaaacagta gtttcggtta
     3001 agatatgtac gtagccgaat cattcaaatt taaagttatt caaaaataac ttcttactaa
     3061 ggaaggcgaa aatatggcat ttattcaagc aaatatttat tcgaatgtgt tagaaatgga
     3121 agtcaatcta aatgtgatac taccgcaaac aacaaaaaag gtgattggca ccagttcaga
     3181 gcaagagaca acggatatac ctgttttata tttacttcat ggaatgggtg gcaatcatag
     3241 tgtatgggaa agacgtacgt cgattgaacg ttatgtagca aactacggat tagctgtcat
     3301 catgccatct actgatcttg gctggtacac agatacagtt tatgacatga agtattggac
     3361 attcattgca gaagagctac ctgagatttg tcatgaatta ttcccgcaat taacaagaaa
     3421 aagagaaaaa acatatgccg ctggattatc gatgggcgga tatggcgcgt taaaactagg
     3481 ccttgcaaaa tcagaaaatt tcgcagcagt aatctctctt tcaggcgctg tttctgtagg
     3541 aaatcgaatg gatgatcttt tgatggttcg taaagcatct ttttgggaag ggatctttgg
     3601 accattggat caaatcgaag gttcaaaaaa tgatccgcaa tacttattga aagaactagt
     3661 aaaaagcgga aaaccagttc caagatttta tatggcttgt ggggaatctg attttcttta
     3721 tcaggcaaat caggaaatgg tatctgctat gcaggaacaa ggcttggcgg tcacctttga
     3781 acaaggacct ggcgatcata actggatctt ctgggaccaa tggattcaac gtgcattggc
     3841 gtggctaatg gaagaaaaat aaaaaaagag tcttaaaaag atagctccta tagagatcac
     3901 aaacatagtg atctctatag gagctttttt actgctcgtt ttctcttctt ttattttgtt
     3961 ggcataagcc ttcaatatag gtttgtgcac tcgttagaaa gagtgctttt tcattcaatt
     4021 ctgcatggga ctgcatactt tctttaaatc cctctaattg attttcaact acataattac
     4081 gcataaatag aactgtttct ttcaaaaatt catttccgtc cataattttt tgtcgaagct
     4141 gctgttcatg ctgctgatcc attaactgtc caatcaataa attgtggaga gaggcaatgg
     4201 cctgatgact ttgggtgact gaaaatcctt cacgaacaag taattggatc atttgctcca
     4261 aatgttctaa tcgactcgga aaagcgggca cagtattgat ttctaattct gccccacaag
     4321 ggaaacgtgt ataaaggtca tagtaatttt ccatatactg cagcaactgt tcattccaag
     4381 gcaaagttgg atctggaagc gctaattctt cttccattgt ttcagccatt ttttgcaaga
     4441 gctgttgttt gtttttgaaa taccaatata aagcaggtgc ttgaacatga attctgtttg
     4501 cgatgtttcg catggaaagc tgcgaaagag cttgtgtttc agccaggata tcaaaggcgg
     4561 cctgaatgat gattgcttga gaaagcttta tttccatttt atcaacctcg tgtattgcat
     4621 tatcttttta aacatggtaa attgttagtc ggtctaattt tacagtgtta aattagtcct
     4681 gtcaatttaa acaaaggggt gtaatcaacg aatgaaatta atcttacgac atgcgaaaaa
     4741 atataaagta gctgttttta tttccttgct gtccgtagca gtcatggttg cagcagcatt
     4801 atggcaacct aagttactac agcaagtatt ggaagctatt attacagaag ataatgatga
     4861 aatgaaaaca attggtattt acctgatcag tctggctttg ctaggattag ccgcaggtgt
     4921 aacgaacacg attttctctg caaaagtagc tcaaggcgta agtgcagata tccgagaaga
     4981 agcatttcga aagatccaga cattttcatt tggaaatatc gaacagttct ctgctggaaa
     5041 tcttgttgtt cgtctaacaa atgatatcac gcaaatccaa aatttagtga tgatttcttt
     5101 gcaatcactt tttcgaattc cattcttatt tattggtgcc ttcattttag ctatgatcac
     5161 gatgccgcaa ttatggtgga tcatcgtagc gcttatcgtt acggtatttc tgattacggc
     5221 actttcattt acaaggatgg gcaagcactt catgatcatt caaaaactga ttgatcgtgt
     5281 gaatagcatt gcaaaagaaa acttactagg gattcgtgta gtgaaatctt ttgtacaaga
     5341 agaaaaccaa ttgaagtcat tcagcaaagt aagtgaggac ttaacaaaac acaacatcat
     5401 agtcggtaca ttattttctg tgatgatccc gtccttcatg ctggctgcca atttggctgt
     5461 ggttggggcg atcttttttg tgagcgatct tgttaaagat gatcctacgt taattggtgg
     5521 aattgcttca ttcatgaact acttgatgca gattatgatg gcaatcatta ttggcggaat
     5581 gatgatgatg atgacttcaa gagcagccgt atcgctgaag cggattgcgg aaattttaga
     5641 tgctgaacca gatcttactt atttagacgt tccagaacaa gagttgacgg gttctgtcac
     5701 ttttgaccat gtttcttttc gttatcctgg tgaggataca gatacgctta aagatatcag
     5761 cttttctatc aaacctggag aaatggttgg tatcgtcggg gcgactggtg ccgggaagtc
     5821 aacactagca caattgatcc ctcgtctatt tgatccgaca gaaggcaaag ttgaagtagg
     5881 tggtgtagat cttcgtcaag taaatgaaaa gagtttaaga caaacggttt cctttgtttt
     5941 gcaaaaagca attttattct caggaacgat tgctcaaaac ttgcgccaag ggaaaaagaa
     6001 tgcaacagaa aaagagatgg aacatgcttc ttctattgct caagcaaaag aattcatcga
     6061 aaagttatct gatcgttacg aagcaccaat agaagaacgt agcagtaact tttctggtgg
     6121 tcaaaagcaa cgtttatcca ttactcgtgg cgttatcggt aatccaaaga tcttgatcct
     6181 agatgacagt acgagtgcac tggatgctcg ctctgaaaag ctggtccgag aagcactcga
     6241 tcgagatttg aagggaacaa cgacgattgt catcgcacaa aaaattgctt ctgttgtcaa
     6301 agcagaccgc atcttagtat tggatgaagg cagattagtc ggcgaaggaa cccatgagga
     6361 attggtagct ggaaaccgtg tctatcagga aatctttgaa actcaaaaag gaacggagga
     6421 gtaagtatga ctgatctaat aaaagcaagt aaatttttct atcactattt gaagcggtac
     6481 aaactctcct ttttctttat ctttatcaca gtgatcattg ctacctatct acaagtaaag
     6541 gcacctcaat acatcgggga agcttttcaa gaattagcta attacgtagg aggcttgatg
     6601 caaggcgtag acgataaaag caaatttgtt tctgtaatat ggaaactctt gctgttttat
     6661 gtactagcta gtgcagccaa ctttatctat agtattttgt tcacacaagt agtagggaag
     6721 tcaacgaacc gaatgcgtat cggtctattc aacaaattgg aaaaattgac gatccgtttc
     6781 tttgattctc atcaagacgg tgaaatttta agtcgtttta ccagtgactt agataatatt
     6841 caaaatagtt tgaaccaagc tttgcttcaa gtcatcacga atgctgtttt acttgtcggg
     6901 atcttgatca tgatgttccg ccaaaatgta gagttagctt gggctacaat tgcttccact
     6961 ccagtagccg ttttgatagc tgtcttgatt atccgcaaag cacggaaata tgtggatctt
     7021 cagcaagatg aagtggggaa actaaatggt tatatggatg aaaaaatcag cggacaacgt
     7081 gtcatcatca caaatggctt acaagaagaa acgattgaag gattcttaga gcataatcaa
     7141 agtgttcgcg aagcaacctt caaaggacaa gcttattctg gtctactatt tccgatgatg
     7201 caaggaatgt cactggtcaa tacagcgata gttatctttt tcggcggctg gttagcatta
     7261 aatggtgatt tggaacgttc ggtggcatta ggcttagttg tgacattcgt ccagtattca
     7321 caacaatatt accaaccact gatgcagatt tcatctggat acagcatgat ccaattagcg
     7381 attacagggg caagacgatt gaatgaaata tttgatgaaa aagatgaagt caaaccagaa
     7441 aacggtaaag catttgatgg tgttgaaaaa ggtcttgcgt tgcagcacgt agattttggt
     7501 tattctccag aaaaactagt cttgaaagat gtctcgatca atgtggacaa aggagaaatg
     7561 gttgctcttg ttggtccaac aggatcgggg aaaacgacga tcatgaatct gctaaaccgt
     7621 ttttacgatg tcaatgatgg ttcagtagtc atcgatggaa tagatatccg agattatgat
     7681 cttgatgcat tacgtagaca tgtaggaatc gttttgcaag aatctgtatt gttctccgga
     7741 acgatcaaag acaatatcac attcggcaaa ccggacgctt cagatgaaga agtcatcaat
     7801 gcagcaaaac aagcgaatat tcatgacttc atcatgacgt tagaaaaagg ttatgataca
     7861 gagatatccg aagaaaataa cttattcagt acaggtcaaa agcagttgat cagtattgct
     7921 cgaacgatca tcacaaatcc atctttattg attttggatg aagcaacaag taatgtagac
     7981 acggtaacag aagcaaggat ccaaaaagca atggatgaag caatcaaagg aagaaccagc
     8041 tttgtgattg ctcaccggct gaaaacaatc ttgaatgcag atagaatcgt tgtcttgcgt
     8101 gatggggaag tcattgaaga aggcagtcat catgagttat tagcagaaaa tggattttat
     8161 gcggaacttt atcataatca atttgtattt gaaagttaaa ttaaaagaac ctcgttataa
     8221 cggggttctt tttatatatt ttatgttgtt tgtttttata aggggca
//
LOCUS       DAKBVG010000120         8235 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000120 DAKBVG010000000
VERSION     DAKBVG010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.120"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            <1..852
                     /gene="mltG"
                     /locus_tag="OUD09_001865"
     CDS             <1..852
                     /gene="mltG"
                     /locus_tag="OUD09_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HCT9693127.1"
                     /translation="NYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVA
                     DGKIAIPEGYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELYQKFPELLESASKAE
                     NTKYRLEGYLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTL
                     ASLVEKEGVKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSP
                     YNLYTNTGYGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELV
                     QKYVNNS"
     gene            988..1467
                     /gene="greA"
                     /locus_tag="OUD09_001866"
     CDS             988..1467
                     /gene="greA"
                     /locus_tag="OUD09_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCT9693128.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGD
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            1635..2366
                     /locus_tag="OUD09_001867"
     CDS             1635..2366
                     /locus_tag="OUD09_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HCT9693129.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKYPRTKFQNFQLILGSLVIFFSLVNSPALWMMVVLAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMVQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAIISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKICSS
                     DYDENPRRLKIITNTLVGDVEVIRI"
     gene            2363..3430
                     /locus_tag="OUD09_001868"
     CDS             2363..3430
                     /locus_tag="OUD09_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCT9693130.1"
                     /translation="MMGRISKAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLMAISLGVGLIVFLLLSLIQKAKYGKIEEKLRALSSGNYESKLLHQP
                     IPSASDDLYIKDIDMEITKIKEKMIEVSSELQIMTSRPQYVDGQTKEEILKLERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWEVEDVKLTSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVETKVPLMKEA"
     gene            3437..4069
                     /locus_tag="OUD09_001869"
     CDS             3437..4069
                     /locus_tag="OUD09_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCT9693131.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     ALRPDVILMDLVMEEMDGIESTKAILKDWPKAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNDPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            4209..4874
                     /locus_tag="OUD09_001870"
     CDS             4209..4874
                     /locus_tag="OUD09_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HCT9693132.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            4902..5195
                     /locus_tag="OUD09_001871"
     CDS             4902..5195
                     /locus_tag="OUD09_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HCT9693133.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(5263..6210)
                     /locus_tag="OUD09_001872"
     CDS             complement(5263..6210)
                     /locus_tag="OUD09_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCT9693134.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(6388..7191)
                     /locus_tag="OUD09_001873"
     CDS             complement(6388..7191)
                     /locus_tag="OUD09_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HCT9693135.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            7337..>8235
                     /locus_tag="OUD09_001874"
     CDS             7337..>8235
                     /locus_tag="OUD09_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HCT9693136.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKNVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLN"
BASE COUNT         2800 a         1298 c         1706 g         2431 t
ORIGIN      
        1 aattattata caaaatttaa aaatttaact ggctttcaag ctggctatta tcagttagcg
       61 cctaatatga ctcttgacga gattggcaaa cagctgcaag aaggcggcac ttctgagcca
      121 acaaaagttg cagatgggaa aattgccatt ccagaaggct acgacatcga tcagattgct
      181 gagcgggtag cgaaggtaac tggtaaagac aagaaagaat ttttagattt agtaaatgat
      241 gagactttct ttaacgagtt gtatcaaaaa ttcccagaat tgttggaaag tgcctctaaa
      301 gctgagaaca caaaataccg tttggaaggc tacttattcc cagctactta tgattactac
      361 acgagtactt cgcttaagga tcttgtgatt gagatggtga acaaaacaaa tacggtcatg
      421 caaaactatt attcagctat caaacagaaa aatttgactg tacaagaagt tttaactttg
      481 gcttctctag ttgaaaaaga aggcgtaaaa gaaaatgatc gtaaaaatat tgcacaagtc
      541 ttctttaacc gaatcaaggc aaatatgcca cttcagtcag atatctctgt tttatatgca
      601 ttaggagaac ataaagaatt ggtgacgtat gaagatacag ctgtagattc accatacaat
      661 ttgtatacca ataccggcta tgggcctggt ccttttgaca atccaagcga agaggcaatc
      721 aaggcagtat tagaaccagc agaaaatgac tattattatt tcgtagcaga tacgagcact
      781 ggaaatgttt atttcgctaa aacgtatgaa gaacatatgg aactagtgca aaaatatgta
      841 aataattctt agaaaaacag gggctagtca ttgatcattt ttagaaaaac atttacattt
      901 taagaaaagc gtggtaatct tttttggact atattcctca aaagattacc ttttgcggta
      961 ttttatcgaa aaaaggagac gttacacatg gtagagaaag tttatccaat gactctagaa
     1021 ggtaaagaaa aattagaaaa agaattagaa gaactaaaaa cagttaaacg cggagaaatc
     1081 attgaacgaa tcaaaatcgc tcgcggtttc ggagatcttt ccgaaaactc cgaatacgaa
     1141 tcagctaaag atgaacaagc atttgttgaa ggaagaatca caacaattga aaatatgatc
     1201 cgttttgcac aaatcatcga taatgacggc gtagatgcag atgaggtatc gatcggtaaa
     1261 acagttacgt tcatcgagtt gcctgatgga gatgaagaag agtacacgat tgtcggaagt
     1321 gctgaagcag acccgttttc tggaaagatt tccaatgatt ctcctatcgc gcaagcattg
     1381 attggcaaac gtaaaaatga tgaagtaacc attgctacac caggtggcga tatgttagtt
     1441 aaaatcgtca aagtagaaca agcgtagtct gactgaaaga tctagagagt tcatcttact
     1501 gatgacgcac tctagatctt ttctctttga gtattgcagg tttcactgaa cgatcttaca
     1561 tctaaagtcc tatagccaac agtcaaaaaa ataggtatga tgaaatgaga atgtgtcagg
     1621 agggagaaaa agcgatgaat agttcttggc gtttttttgt ggtagtcgaa gcactgctgc
     1681 tgttatttgc tgtttggcaa atcgtaaaca atactggact tttgctttta gtaatattcg
     1741 gtattttcaa tatatatcta gctatgcgca aatatcctcg tactaaattt caaaattttc
     1801 aattgatctt agggagcttg gtcatttttt ttagcttagt caacagtcct gctttatgga
     1861 tgatggttgt attggccgtt ttatttatcg ggctgaaagg tgtagagatt tcaggaatag
     1921 atttgacaaa aaatgctttt tggcgaaaaa aacagatcat gatggtccaa acagagcaat
     1981 taaaaacaca taataatgag cgtaaaagac agcaattatt tggtaaccaa cgtattggaa
     2041 acgacgtcta tgaatgggat gatatcaaca ttgccatcat ttctggtgat actatcatcg
     2101 atttgggcaa tacacttttg ccaaaagatg ataatattgt gattgtaaga aaagggatag
     2161 gcagaacacg gatcttagta cccctgggag tggctattcg tttagagcat gcgacattag
     2221 taggaaatgt cttgtttgaa gaagagcagt tttccttaaa aaacgaacag atcaaaatct
     2281 gcagcagtga ttatgatgaa aatcctcgca gattgaagat cattaccaat actttggttg
     2341 gagatgtcga ggtgatacga atatgatggg aagaatatcc aaagcaatgc tagctgttta
     2401 ttcggggatt gcggctttcc ttattattct attttcactt ttcacttatt tttatgccag
     2461 taaccaaagt cattggtggg gagaattgct gcgtgcgcgt ttattatacg ttccgcttat
     2521 cttccatttg atggcaattt cattaggtgt aggattgatc gtatttctat tgttatcact
     2581 tattcaaaag gcaaaatacg ggaaaatcga agaaaagctt cgtgcacttt cttctggcaa
     2641 ttatgaatcc aaacttttgc atcagccgat cccaagcgcg tcggatgatc tgtacatcaa
     2701 agatattgat atggaaatta ctaaaataaa agaaaaaatg atcgaagtat ccagtgaatt
     2761 acagattatg actagccgtc ctcaatatgt agatggacaa accaaagaag agattttaaa
     2821 attagaaagg catcgattag ctcgtgagct tcatgattca gtttcacagc aattatttgc
     2881 agcgatgatg atgatgtcgg cattgacgga gcaagcagaa aaaagtgaga caccagaaat
     2941 gttccgtaag cagttgaaaa tggtggcaga gatcatcaac gcttcccagt ctgagatgcg
     3001 tgcgctgctt cttcatctgc gtccagtcaa tttagaagaa aaaagtttaa aacaaggcat
     3061 tgagcagtta ttgaaggaat tgcagaataa aatccagatt tcgctaaaat gggaggtaga
     3121 agatgtaaaa ttaactagtt ccattgagga tcacttgttc cgaatcgttc aagaattgtt
     3181 atcaaacaca ttaagacatg ctaaggctaa tgaattagaa gtctatttgc ataaaataga
     3241 taacaatctt ttattacgta tcattgacga cgggacagga tttaatatga atgaaaccaa
     3301 aacgggaagt tatggattga acaatatcaa agaacgagta gctggtattg gtggaacagt
     3361 aaaaatcatt agttttaaag ggcaaggtac gagcgtggag accaaggtcc ctttgatgaa
     3421 ggaggcatag ataaagatga taaaagtttt attagtagat gaccatgaaa tggtacgctt
     3481 aggcgtctct tcttatttat ctatccaaga agacattgaa gtgattggag aagctgaaaa
     3541 tggacgtcaa ggctatgaaa aggcgatggc acttcgaccg gatgtcattt taatggatct
     3601 agtcatggaa gaaatggatg gcatcgaatc aacaaaagca atcttaaaag attggccaaa
     3661 agccaaaatc attatcgtca caagttttat tgatgatgaa aaagtttatc ctgcaattga
     3721 agcaggagca gcaggatatt tgttgaagac gtctacagca catgaaatag ctgatgctat
     3781 aagggctacg caacgcggcg agcgagtact agaacctgaa gtgacaacaa aaatgatgga
     3841 aaaaatgagt cggcgaaatg atccagtatt gcatgaagaa ttaacgaatc gagaaaatga
     3901 aattttgatg ctgatttctg aagggaaaag taatcaggaa atcgcggatg aattgtttat
     3961 cacactgaaa acagtcaaaa cgcatgtgtc gaatatatta gctaagttag aggtagaaga
     4021 ccgaactcaa gcagctatct atgcgtttaa acatggtttg gtgaaataaa aacgatgata
     4081 aagaggataa tcaaaagctg agaaaataac tattattttt cttggctttt ttacaataat
     4141 gatgcttgct tttgtttttg gaaaggcttt gttatactga gaaaagaaga tactaaagga
     4201 ggaaaatggt gaaacaaaat tttgcaattg taggccttgg acgatttggc ggaagcatct
     4261 gccgtacact gatcgaagct ggacaagaag tactggctat tgacagtaat gaagatcgag
     4321 tcaatgaata tatgaatata gccacccatg ctgttgtggc aaatgcacag gatgagatga
     4381 cacttcgttc attaggtata agaaatttcg atcatgtaat tgtcgctatt ggagaagata
     4441 tccaagccag catcttagtt actctgatgg taaaggaaat gggcgtaccg aatattttag
     4501 caaaagccca aaatgagtac catgcccgtg tactagagaa gatcggagca gatcgtgtcg
     4561 tacaccctga gcgagacatg gggatacgga ttgcacataa cttagtatca aagaatattc
     4621 tagactactt agagctgtca gataaatatt ctttagcaga aatccgagtc tccaatccta
     4681 aatttttcaa caaaacatta gctgaattaa atttcaggca acgttttgac ttaacagtag
     4741 tcgccatacg aagagtagat caaacggtta tcgcttctcc tgcagcagat gaatatgttc
     4801 gtgaaaatga taatctactg gttattggag agacaggcga tgtggatatt ctagacgata
     4861 agatgaatca atagaaagaa aagagaaagt aggttgttgg gatgaaatta acggtaacgc
     4921 caaaagcaca aaaatggttt gactcagaaa ttgatctgcc agaaacttac ggaatacgtt
     4981 tttttggaaa agtctacggc aaaacagagg ttcatgatgg tttttcgatt ggaatgtcag
     5041 ttgaacagcc agaacatcct gtaaagaaag tggaaatgga tgggacactt ttttttatcg
     5101 aggaagcaga tgaatggttc tttaaaggat atgatttact tgttgattat gatcccaacc
     5161 tggaggagcc aacctatcac tttaaaaaac aataaaaaaa gagagggtga cacaattttt
     5221 gtcaccttct cttttttctt ttgaaaatat tgttttcagc tattaatatc ctgcagacaa
     5281 ttttacttga ttggtttgaa gctcatgggt ggcaaagtag cttttcagat tggttaaaaa
     5341 aatagccatt aatttctttt gaaaatcagg tgtcaaacct gcaatgtggg aagtaatcag
     5401 tacattgtcc attttccaaa gaggatgatc ttgtggaaga ggctcttcct caaaaacgtc
     5461 taatgctgca aaggaaagtt gctggttatt taaagcctgc accagatctt gtgtcttaac
     5521 agaagcacca cggccaacat tgatgaatac agattttgga tcaaagtgtt caaacatatg
     5581 ctgatcaaag agcccagttg tttcctccgt tagaggtaag atattgacta cgaaatctac
     5641 ttcaggaaga acttccttta gttgatttaa agaaaaagtc ttttccattc cttctgctgg
     5701 atgaccggat gtattgattc catagcagtt catgtctaat gctttgcatt tttttgcaat
     5761 catttttcca atatggccag ttccaatgat tagtattttt ttcccttgga tagattggta
     5821 atggaccgat gaacctaacc atttcatgtc actctgtgcc ttttgtgctt gaaaaagacc
     5881 acgagaatac ccaaggatga ttcctaagac atgctccgta atcgattcac tgtggatgcc
     5941 actaccattt gaaagtaaaa tatgtttctt tgaaaaagtc tcaaggggta gctgatcaac
     6001 accagctgag atagattgga cccatttcaa ggtagaagag gctaatagtt tttcttggta
     6061 gtctgagttc cagcccaaac tgatctctac attttgtaga tcctctggtt gtaattctgt
     6121 tttaaagata taaccaggcg ctatagtatg gatttcttcg ataaattcat cacgaaatgg
     6181 acgatctgca taaatgattc tttctgacat agttaatttc ccgcctttct ttactctaag
     6241 tgtagcgcat acaagaagaa ctttgaagcg ccttgcttgt tttttgagaa agaaatattt
     6301 ttcaaataaa aaaggggctg tgacaaaaaa atgtcacaac ctctaaaagg gtctatttaa
     6361 gtagaaataa cgagttgtct ttatttctta attgatcgat atttttgccc atcctttgac
     6421 agcttgagca aaaataaaga ttcctgcaaa agcaatgaag gcgctagtgt caaaagaagg
     6481 aagaaagacc atcaagattg ttgcaattat agtaggcaat gaaattgaat agatcaatgt
     6541 ttttaaacaa tcaagaaaag tagcttttgt tagtcgaaag cgtgcataga gaaaagcagc
     6601 aatgttggta aatagcaaag tgatcagcag attcagaaaa ctaggataaa tagaaaatat
     6661 aaatgtcagg acttttatcc aaagtggtat agatgcttca cttaaaaaag aacggagctg
     6721 cttacctgtt aagtttttta aagattcatt ggtgtaggga agttctaatt ggttattgtt
     6781 caacaaagca gaagtcgtgc ctgtattagg aagagctatg ataagtttgt tttttaacat
     6841 cccgacacta acaaaattcc ccatcaaatc agtagagatg tcctgttctg ttcgttttcc
     6901 ctctggatca aatgtgaaga taatggagtt tgtttggtaa atgaatcctt ctttttcttc
     6961 ggtttgaagc tgcccgtttt ggattgtaaa atcaggaatg cgagtagcaa tcttttgccc
     7021 atccaattga atgttattta agacttgaaa aacttgaaaa gagattggca aagccataat
     7081 tatactcaaa actaaaagat agacaatggc tttccaaaat gggacatttc tcgcctcctt
     7141 taggtctttg aatcgtataa aggaactgat aattaattgt tttgttgtca taagtactcc
     7201 tcatctagta gtgtcatcac ttgcaattct agcgaagtat caagtgaaat acaagtgctt
     7261 tagggaaaca tctgaatgat tggtgaaata tcaaaaaaat gttattcttg tctaaagact
     7321 atccaatata aataaaatgg aggaaggaat agtgcctaca cagccacaga gatcatcaaa
     7381 aaaactattt ggagtagtgg ttttgaatgc ggtgatcact ttatcagaat ttattggagg
     7441 gattatgtca gggagtttgt ctcttgtttc agatgctttc cataacctta gtgatacatt
     7501 ggcaattatt ttttcttacg gggcacaaaa aatggcccga aaagaagcaa ataaaaaacg
     7561 tacgtatggc taccagcgtt tagaaatatt gagtgctttc atcaactcat tcatattgat
     7621 catattatcc ttgtttttaa ccgttgaagc attcaagcgg ttcacttcac cagaaaagat
     7681 aaatagtcat ttgatgctaa cagtagcagt tattggattg ctcgcgaatt tgttctcagt
     7741 attattatta cgacaagaag cagatgaaag tttgaatata aaatctagtt atttgcattt
     7801 gttaagtgat acactgtctt cggtaagtgt gattatcgga gcaattttga tccgtttttt
     7861 cgatatctac tggatcgatc cagttattac actgatcatt tcgatatata tattgataga
     7921 agcgataatg gtgatcaaaa aagctgccgc tattttgata caaagtgcac ctgctattga
     7981 ctatgaaaaa atggaacaag aaattgaagc aatcgatggg gtaaagaatg ttcatcatgt
     8041 ccatatttgg cagtatagcg aaaagataat tattttcgac ggacatattg attttgaaga
     8101 tcagctacta agcgagattg aaaagatata tcctcgtata atttctttat taaaattaaa
     8161 atatggtatt acacatgtga cgatccaagc agagacaaag gcaaaggatc aaaaaaaatt
     8221 aattttttta aataa
//
LOCUS       DAKBVG010000121         7988 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000121 DAKBVG010000000
VERSION     DAKBVG010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.121"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            1..645
                     /locus_tag="OUD09_001875"
     CDS             1..645
                     /locus_tag="OUD09_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCT9693137.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(701..1204)
                     /locus_tag="OUD09_001876"
     CDS             complement(701..1204)
                     /locus_tag="OUD09_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HCT9693138.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(1217..1263)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1801..2766
                     /locus_tag="OUD09_001877"
     CDS             1801..2766
                     /locus_tag="OUD09_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HCT9693139.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(2865..4037)
                     /locus_tag="OUD09_001878"
     CDS             complement(2865..4037)
                     /locus_tag="OUD09_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT9693140.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(4024..5169)
                     /locus_tag="OUD09_001879"
     CDS             complement(4024..5169)
                     /locus_tag="OUD09_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT9693141.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(5183..6115)
                     /locus_tag="OUD09_001880"
     CDS             complement(5183..6115)
                     /locus_tag="OUD09_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT9693142.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            6232..7311
                     /locus_tag="OUD09_001881"
     CDS             6232..7311
                     /locus_tag="OUD09_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HCT9693143.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            7308..7937
                     /locus_tag="OUD09_001882"
     CDS             7308..7937
                     /locus_tag="OUD09_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCT9693144.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
BASE COUNT         2572 a         1643 c         1405 g         2368 t
ORIGIN      
        1 atgctattaa aaacacttgt atataagaaa aaagatctga caaccgttac agagactagt
       61 acattagaag aagcattaaa aattttagaa acttccggat accgttgtgt gccgatctta
      121 gattcttcag gtaatatttt ccgtggaaac atttacaaaa tgcatatcta tcgtcataaa
      181 gcaaatggcg gtgatatgac gcttcctgtc actcatcttt tgaaaaacgc tacaaagttc
      241 atttacctgg atacttcttt cttcaaagta ttctttacga tcaaagagct tccttatatt
      301 gctgtccttg acagtgaaaa tcatttttat ggtattttga cacatagtac gttgcttaat
      361 atgctctctc aatcttggag cgtagaacaa ggaagctatg tactgacaat tgcttccaca
      421 ggaaagcaag gagatttggc tgctattgca aaaatcattt caaaacacag cagcattgcc
      481 agctgtatta ctcttgatat cgaaaaagac gaatacattc gacgtacatt gatcacgcta
      541 ccggctaaca ctgaagcgaa agtatgtgaa acgatcgtca accacttaga gaaaaagaat
      601 tataaagtgg tagaaattga aaatttgcaa aaccaagaag cataatcaaa aagcgaaaaa
      661 agaaaacagc taatctgctt tcttttttcg ctttttataa ttagacacgc ttttcaaaat
      721 caagcgcgcg atctaaataa tacatcaatg gtgctgaaac agccatgacg ataaactcac
      781 ttaatgcaag cgtgccatag gttgtccaaa atgcttggcc acctgttgga actaacatat
      841 aagcaatcag tcccatgctg atggtaaaga agatcgtgtt caatactaaa cgcatcttta
      901 catttttcac tttgttttgc aataaagctg tcaggcctaa agaaagcaat gtttggccgc
      961 caccaaaaac cacatccatg ataccaaaac caaagaaggc attatagacg atgaccccgc
     1021 ctaaaactcc ccaaagcaat ttacggttaa agacaactaa atgattcaaa ctttctgaaa
     1081 tacgaaactg gatcggccct gaggatacag gcgcaactaa aatagttaac gctagataaa
     1141 gagccatgat gatgccattt acaacgatta cggataaccg ttgtctgctt ttcgtttttt
     1201 gcattttctc tcctcttttc tcctagtttt tttaacgcgg gatggttgat gaaccgcggt
     1261 tatgtgaaac aaacaataat tataacagac ttttcattta tttcaatccg tcttatctgc
     1321 ttttaaaaaa gcaaaagctt aaacaatgta aacgtttgct aaaaatcgtg ggatatggta
     1381 taaagtaagt gtaaggggtc ataagaattc ccagtaaagc agacgaagta ggatctgcaa
     1441 gaacagaatt gttgtgaaac ttaaataaat aaaaaaggat gtgggaattt gagcaacctc
     1501 ttactagaga acggtctagc aagcagctga agatctaagt ggacatgccg aaccttggaa
     1561 cttctcctta cgtaagtaat ggatcatgcg cttgctaggc ttttttgtgt ttttcggtct
     1621 tcggaccttt ttcattttcc tagacttctg ctataataac caagaattaa ttttgtaaca
     1681 gatttttttg aaaaaacaag tttctttcat tcaaaaaata gaaaaagctt gctaattatt
     1741 ggttaaccag caaacaggag tattaaaata tcttgtttta ttgaatagca ggaggaaatc
     1801 atgaaattcc attatcatat attgatcaat ccatcggcag gcagcggaaa tggctataaa
     1861 gtagcagaaa gaattttacc ggtattaaaa aataagcata ttgactatac acttcattat
     1921 agtgaataca aaggacacga tgctgagatt gctgaaactt tggcaaaaga aacattgatt
     1981 ccttggatag aagaagaaca gaaaacaatc gatactttcc cccttttgat catcgtagga
     2041 ggtgacggca ccttacatca ggtacttgat actttctatc agatggaagt agaattccca
     2101 gttgcttata ttccagcagg ttctggaaat gattttgctc gaggggcagg ccttccaaaa
     2161 aatcccaaaa aaggacttca gctgatttta gcagctcaat caccagaaaa agtccacata
     2221 atggcttatg aagaaaaaat aagtgaaaaa aaaggaatag ctgttaataa tttcggtata
     2281 ggattagatg cagctatcgt ccatgcgaca aatcattctt ccacaaaaaa gcgattgaac
     2341 aaatacaact taggatcatt ttcgtattta ttctctattc tccgtgcttt attcactcaa
     2401 aaaggatttc caattctggt agatgcagga ggaaaacaat taagtttttc aaacgcattt
     2461 ttatgtacag caacgaagca tccttttttt ggcggaggaa tcgcgattgt tccaactgct
     2521 gatgcgtcta aaccagtcat tgattttgtc gtggtcgaac gaatcaattt ctttaaaatc
     2581 ttgtggctca ttcttttact tatacagaaa aaacaattga aatcgaaata tttccatcac
     2641 tacacaacaa acagattacg tatcgtttca acaacaccgc aacatggtca agaagatgga
     2701 gaagaaatgg aaaaacgacc ttttgatatc acaataacca caactaaaca gttgttttgg
     2761 tgttaataaa agaggttttg tcagaagcta catctggaac accgtttatt cggaaaaaag
     2821 gactgtgaca cgacttttgt cacagtcctt tttttactat gcagttaatt ctttgcccaa
     2881 ttgggttgcc atcgatagtt tctttcttta cccaaaataa attggatcat tgaaaacacc
     2941 gcagcaaaag caactaagac acaaccattg aaccaaaaag attctgcaaa tgtttgattg
     3001 taactgagtg tttcactgat caagttatta ttttgaacaa accaatatgt tggtgtgaat
     3061 gccgctattt tgctaaccat ttctggcaag tattctgctg gtacaaacac cccaccgata
     3121 aagcaactgc ctgtgataaa aatatctttg atcccaccgc tggtttctga gttttttacc
     3181 aatgaagtga tacaagccga aaagctaatg atcggcaata aaaacaagaa agtatttaaa
     3241 atatgatacc ataccgttat atcccacgag gtgtggatca ctaacagaac cattagaagt
     3301 aagaagccac tgaacaacag cagcgagtaa ctaatcaaac taaaggttac tttgcgattc
     3361 aagtttcgtt tggtaatggg cgcactctgg tttcgtttac tgatctcttt gcgattaaac
     3421 gctagattga tgacgctgaa accgctaaaa atggatagaa acaacccata agacaagaaa
     3481 ttaaaggttg tgcccgtcaa cagctttttc atccgctgac tatatcccgc acttagctca
     3541 acttctccag taaccgagag tgccatttgt gtctgttcca tagctgcatc gattgtttta
     3601 tcttcttctt gaaacagaat cagtgtttgc aaaaaggtgt tcacttgctg tgtaacaact
     3661 gttttagaaa aggcatctgg gcgactctgt atcttgatcg tcgccatctt tcctacttcg
     3721 acttgttcgg taaagtctgc aggcagatgt aagataatgt ttacacgatt aaaataaaga
     3781 gcatcatcga tttctttttg tgaggttcca tccaatgtaa cgactgtttc cttgtcattc
     3841 aaatatttac gaaggccatc gactacaggc gactcttctt cactaatcaa ggcgattttc
     3901 gcatcttgca gagaggtatt ttcaacatca atattttgcg caaaaaagaa tgtggcaaca
     3961 acagtgataa ttacacctat cagtaaattc gatttatttg ctttaagcaa tgtccaaatc
     4021 gttttataaa gcgtcataac gcacccctct ttccatccaa gcatttccta acgcaaaaag
     4081 gaaaataaat ccgatcaacc atagaagatt ggtataaaaa ctagccaaat cagtgtagaa
     4141 ataaagttta tacaaacttt cgctgatcaa gttgacgaga tttaattgac caacaatcgg
     4201 aatatgaaga tcaatccaat acttcacttg ttccgacccc atcataccag caaaaaagct
     4261 catcgcaatc gtcactgaaa tcaagatgct atccttttgc accagattca tttttggtat
     4321 caaattacca accaatgtac ctaacaaaat cgcacacaac gaccccagag cggtaacaat
     4381 catcaaccat tgccaacgtt caccaaagtc tacttgatag ataaaacgaa agacagctaa
     4441 cataatataa atctctgtca aaacaatcgt aaagcttgct gccaaattag cgattgaaac
     4501 taacgattgc tttctaggaa tcaaggacaa gcgaatacca tttgacgatt gatttgcttg
     4561 ctgatcctgt acattgcgga ccccccacat cgtcccgtag agaatcgcca ttccaatcag
     4621 agtgaagaaa taaaaacttt tgaaactcat attacgacct gcatgaactt cttgaaaaga
     4681 tacttcttgt tcaaaaacag tcatgatggg cgcgagatca acgttctcca cttctgccac
     4741 tttactcgaa attataccaa tacgctgtaa aaattgatcc atgacgtttt tcatcaacgt
     4801 ctgactcatt cctgcttgac ccacatggag cgaaacagat gaaccatcaa tgagataaaa
     4861 accagccaaa tcatctgcat ttagcttttc attcgcttct acttccgata gttcctgtac
     4921 ttcaaataat ggtttatcat tgacttcgac ttgttctaag actgtggtaa aattttccga
     4981 aagcgctgtt tgatccgctt tgattacaat tccagtatta atcgtttcaa gattgccacc
     5041 ttgatcaatc tcgccaaagg ccgccataaa catcaatcct aaaagaattg gaaaggccaa
     5101 tgtccaaaac aacatggatt tatttcgcag cagtattttt gtgcgatatt tataaaaatg
     5161 ccaaaacatt atttcccctc ctctagtctc tcaaatcctt gccagtaatc tctaaaaaga
     5221 catcattcaa ggacgcttct ttggttgcta tttttccgta actgatcttt tcttcttgta
     5281 agattgtaag aatcggcaac aatactgtat ttagttcagt agttgttact tggagctgac
     5341 cttttttata agcgattcca tcagaagcta ttgttcgctg aatcgctgac aacacatttt
     5401 ctgggagtaa tgggatgtcg atcgtcaaac gatccgccgt ccgtaccata tcttttagag
     5461 attctttggt tcctttagca atcacctgtc cttgatccat gatcacgatc tggtcgcaaa
     5521 gaatttctac ttcttccatg taatgtgttg tatagacaat ggttgctcct tgtcgattca
     5581 gctccttgat actttctaaa attttattcc gactttgggg gtctacagct actgtcggct
     5641 catctaaaaa aattaaccgc ggcttgtggg caatcccaca tgcgatgttc aaccgacgtt
     5701 tcaagccacc actaagttct cctggacgaa acgaaacata tttttctaat cccactaatt
     5761 gaatcacctc ttcaatcaag gctttccttt tctctttttc ggaaacatat aaaccgcaaa
     5821 aataatcgat attttcttgc acacttagtt cgtcaaagac cgcaacgtct tgaggaacga
     5881 ccccaatcat tgccttgaca gcataatttt caggagtcat tgttgtcccg aacaatttaa
     5941 tggttccttt gtcataattt aacaaagaaa gcatacagtt gatggcagtc gatttcccac
     6001 tgccgtttgg acctaacaga cctaagactt ctccttcttg tacttgcaga tcaaaatgat
     6061 tcaatgctac aaaatctcca tagcgtttga ctaatttatc aatttcgata atcatcatga
     6121 aaaactcctt ccaaatttct tgttgatctg actataataa agtgcaagaa aagaaactag
     6181 tgacgattgg ctaaaccaca catgacattt gtcacaatga aggagtctgc tatgcataaa
     6241 tctcggctac tcaacgaaac tagctttcta ctggttatct cattgctttt gctggtaagt
     6301 caaggattcg ttgctatgca agttatctat ttattgatcg ccgttttatt cactggtgtc
     6361 agttatttac tgcctacaaa gccaaattgg attttccaag ttattctatt tcttttatgt
     6421 gcctttttac ctgatcttgt cctgtttctg ccagcgacga tgcgagtgat cgcccctgaa
     6481 gaaagccacc ttgattactt gtttttgtta ggttttttgt tgatttcgac ggttggtaat
     6541 catcccccca tcaaggtcgc ctttgtcgta attttgctgt ttttttcctt ttacttacga
     6601 aaaaaagatc aacgaacgac cgatcttgag aaaaagtaca ttgtctccaa agatactcat
     6661 tgggaaaaac aacaagagtt atcggaacaa aataaagcat tggccctgtc acaagaaaca
     6721 caattagcct tgcaaattgt ggaagaacgg aaccgaattg cccgagacat ccatgacaat
     6781 gtcggtcatc tgctgtcaag tgcgatttta caaattggtg cgcttgaaat gatcaatcaa
     6841 gaaccaaaac taaaagaacc attagaaaat ttaagtgcca ccgttcacac tgggatggat
     6901 cggattcgtc aaagtgtcca tgacctccat gagacatcga tctcttttca acactcctta
     6961 gagtttctga tcgctgattt tccttcttct gttacgattg aaggaaactt gtttgaagga
     7021 ttgaatgaaa cgcagcaaaa ctgcttctta atggtcatca aagaagcctt aaccaacagt
     7081 atcaaacata gtcatgccaa aaccgttgtt ttacattttc gaacgttgcc ggccttttat
     7141 cgcttgcaaa tcacgaatga tggcaccagc ccacaaaaaa attctgctcc cggtattggt
     7201 ttgaaaacga tgcgtcaacg tgttcaagaa caaaagggac aagtccatat tttccaagaa
     7261 aaccagcaat ttcagttaac gatcatttta ccaagggagg gacaccaatg ataacagtag
     7321 tcattattga tgatgatcca tttgtgacga aatcgttaca aaccatttta gaaagcacga
     7381 aagagatccg tgtgttaggc attggtcatt gtgccaagga tgcgctagtc ttatacgaaa
     7441 cccatcgtcc cgatgttttg ctgacggaca tccgcatgcc taaacaaacg ggcatcgatg
     7501 ctgccaaaga aattttggct aaatttccca aagccatcct tttgcttttg acgacattca
     7561 aagatgaaga atacatccgc gaagcatttt ccatcggtgt caaagggtat ttaatcaaac
     7621 agaacttgca agcgattatt ccttctgtca aatccgctta taatggacag gccgtatttg
     7681 gtaatgaaat catcgagact ttcactcaaa tgatgaagaa taaaccgacg attgatagtc
     7741 atgcttatgc ttcttttagt gaacgtgagc ttgcaatcat caaagaagta gctgccggga
     7801 aaaacaataa agaaattgca gatgccttgt acttgagtga cggcactgtt cgcaattaca
     7861 tcagccaatt actagaaaag ctagatttac gggatcgcac gcaattggct atctattttt
     7921 atcagcattt gcaataagtc ttatctaaca caaaaaaacc tgaatgatgc tccttcgcat
     7981 cattcagg
//
LOCUS       DAKBVG010000122         7954 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000122 DAKBVG010000000
VERSION     DAKBVG010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.122"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            374..1237
                     /locus_tag="OUD09_001883"
     CDS             374..1237
                     /locus_tag="OUD09_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HCT9693145.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1366..2988)
                     /locus_tag="OUD09_001884"
     CDS             complement(1366..2988)
                     /locus_tag="OUD09_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCT9693146.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3237..3758
                     /locus_tag="OUD09_001885"
     CDS             3237..3758
                     /locus_tag="OUD09_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HCT9693147.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(3944..5674)
                     /gene="spxB"
                     /locus_tag="OUD09_001886"
     CDS             complement(3944..5674)
                     /gene="spxB"
                     /locus_tag="OUD09_001886"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HCT9693148.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5839..6657
                     /locus_tag="OUD09_001887"
     CDS             5839..6657
                     /locus_tag="OUD09_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HCT9693149.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6760..6957)
                     /locus_tag="OUD09_001888"
     CDS             complement(6760..6957)
                     /locus_tag="OUD09_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693150.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7126..7704
                     /locus_tag="OUD09_001889"
     CDS             7126..7704
                     /locus_tag="OUD09_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HCT9693151.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7799..7936
                     /locus_tag="OUD09_001890"
     CDS             7799..7936
                     /locus_tag="OUD09_001890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693152.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         2377 a         1441 c         1574 g         2562 t
ORIGIN      
        1 aacggcaggc tcacaacctc ttggtaaacg gtgttcaatc agctgaccct cggtattagc
       61 tcaaaaaacg gcaaaatatg aggaactatt ttttccgttt ttttctctaa tactcaggtc
      121 aaaacgctga tttcacaacc tctgattcac ggtgttccag aagtagcttc tacggaaata
      181 agccgaaatt tcacaaaaat ttgagaagca attttcgtaa attccttctt atttcttgaa
      241 gctgaccact tctgtcacaa cctcttattt tgattagaac ttgcgaaaat aaaccggtta
      301 aagtgtgtct agcgcaagag atttgctata atagacgata agggtaaaat gtaacgagaa
      361 ggggttgaaa aatgtgtatc aaattgtaac agattcttgt tgtgatctgc cataccatgt
      421 attagcaaaa gaaaaagttg attttttatc aatgaatctc caattgaatg atcaagagtt
      481 agtagatgac ctcggaaaga cattcaatta tgaccgctac atgaatcagc tgaaaaatgg
      541 tgcgatgcct acgacatcac aaatcaatgt tggccgatat ttagaatttt tccgttctta
      601 tgcggaaaaa cagattcctg ttttatatgt ggctttttcc agtggattaa gcggctctta
      661 cagtagtgcg cttcaagcgg ttgaattgct gaaggaagaa tatgaagacc ctgagatcta
      721 tgtttttgat acaaaagctg caagtattgg ggaagggctt ttagtattgg aagctgtaag
      781 attgaaaaaa gaaggtaaaa gcatcgaaga aaccctagag tggctggaaa gtaattattt
      841 gaaagtccat tcatgggtaa cagtagacga tctgaaatat ctagaacgag gcggtaggat
      901 ttccaaagca agtgctactc ttggcagtct attgagtgta aagcctatta ttacggtgga
      961 taaagaagga aggctgacga atgttgacaa agttcgagga agaaacaagt cgatccagac
     1021 aatcgtcaat gagacggtaa aaacaatcgt tgcaccactt gatcaaatcg tttatgtggc
     1081 ttacgctggg gatctagaga gcgcggagaa ggcaaaacgg cttttggaag agcagataaa
     1141 gatgaaagat gtgaagcttt atcccttagg accaacgatc gccagtcaca caggctatgg
     1201 ctgtatcgcg attttttcga tgggaatcag tcgataaaag cagtaactca ttctgtaaaa
     1261 aagcccagaa attgattgta acaaaaacag cgactcttaa aagatagatg agcatcctat
     1321 ctcttagagg tcgctgtttt tttatttttt aagctaaata ggccattatg cccataactc
     1381 gttcaattgt ttttgaatat ctgcattttc caaaaattca tcatatgtcg tatcagctcg
     1441 atcgatcaca cctttatccg aaacggcaat gatgcggttt gccaatgttt ggataaattg
     1501 atggtcatgt gacgcaaata agattgagcc agtaaatgcc atcaagccgt cattcaatgc
     1561 tgtgattgat tctaagtcta aatggttcgt tggatcatct aatacaagta cgttagcttt
     1621 cgaaagcatc atttttgaaa gcatgcaacg gactttctct ccaccagata aaacattcac
     1681 tggtttcaat acttcttcac cagaaaacaa cattcttcct aagaagcttc ttaagaaggt
     1741 gttatcatct tcttctttac ttgcatattg acgcaaccag tctaaaatcg ttagatcagt
     1801 atcgaattct ttcgttgtat cctttggtaa ataagaatta cttgttgtca caccccaacg
     1861 tactgttcca gtatctggcg taatttcacc catgataact ttgaatagcg ttgttgttgt
     1921 tatatcattt tctgcgacga aagcgacctt gtcttctttg ctcaaattaa aagatatatc
     1981 atctaaaatt ttctttccat caatcgttac acttacattt tctacctgca ataagtcgtt
     2041 tccaatctca cgttcaggtt tgaatccaac gaatggataa cgtctagaag aaggttggat
     2101 atcgtctaaa gtaatttttt caagcatttt tttacgtgaa gttgcttgtt ttgattttga
     2161 tgcattcgca ctaaagcggg caataaattc ttggagttct ttgatttgtt cttctttttt
     2221 ggcatttgcg tttgcttgaa gtttagccgc tagttgacta gattccaacc aaaaatcata
     2281 atttcctaca taaagtttga ttttaccaaa atctagatcc gccatatgtg tacatacttt
     2341 gttcaaaaag tgacggtcat gggatacaac gatcacagta ttttcaaaat tgatcaaaaa
     2401 ttcttccagc caattgatcg attggatatc cagaccgttt gttggttcgt ctagcaaaag
     2461 aacatcaggt ttaccaaata gtgtttgtgc taataacact tttacctttt gtccaccagt
     2521 taattcactc atttgctgat ggtgcaattc ttcagggatg ttcagacctt gcagtaaaac
     2581 agctgcttct ggttcagctt cccatccatt aagctcagca aattctcctt ctaattcagc
     2641 tgctcgaata ccatcttcgt cagtgaaatc ctctttcatg taaatcgcat ctttttcttt
     2701 cagcacttca tacagacgct tgtgtcccat gatgactgtt tcaagtacag tatagtcttc
     2761 atagtcgtaa tggttctgtt tcaatacggc catccgttca tctggtccca tagtaacgga
     2821 acctgtggaa ggctgcagat cccctgataa cactttaagg aacgttgatt tacctgcacc
     2881 atttgcacca atcaagccat agcagtttcc gggggtaaat ttaatattca catcatcaaa
     2941 tagtttgcgg tctgaaaagt gcaaactcac atcatttaca gtaatcaatt tttttcctca
     3001 cttttatcat tattagtttt gacaagtctt atagaattat ataggggaaa ggtaaaagat
     3061 tcaaggcaat cgaacattct ttgtgtttca cgtgaaacag aatgaaaaaa ataaaaataa
     3121 aagcggagcg aaaatagcta ctataggatt tattagtttg caacagcata atttttgacg
     3181 ttatttataa gagggagtac tataaaactg taaaagaaat gaaagaagga ttgaaaatgg
     3241 atgaaaaaag aaccatcgac tggggctcat tgattttggg aattttattt gtacttgtct
     3301 cattgttatc atttcaagac cctgtcggaa atttagttgc aattgtagta gtctttgcga
     3361 tttttgcctt tattaaaggg atatttgaac tgtttgtacg gaacagatta aaagagctaa
     3421 caggatataa aggaaagatg cccttaataa tcgggatcat cgatattcta gttggcgtct
     3481 ttttcttatt caatattggt gcaggagtcg cagcattgcc ttttgttttt gcagtatggt
     3541 ttattgctga ttcagtttta gcgttattta ctgctgactt ggcaagagga gtaagtgaag
     3601 gatattattg gtttacgatc atcgtcaata ttttaggaat tttattagga attttcttat
     3661 tgtttaatcc aatttcttct gcgttgacat taagcttttt agttggattc tattttatgt
     3721 tatttggtat cacacatatc gtctatgcat ttagataagt aaaatcaaac aaataaaaaa
     3781 gccggcttcc gattacagtt ttactgttgt aattggaagc cggctttttt atcatggaca
     3841 aatatgcacg aggtgatttt taggtgtcga gacaaattta tgcaaaaatg tgagaagcta
     3901 tatacacaaa ttttatctta atactcgaag catagcgcag ttgtcacaac ctcttttcat
     3961 ataattcatt caatactggc aaatcatgga cttcatattt ttctttgaat gcttggattt
     4021 cttcttcaga atattttctt ggatctaaat gcagatcttc tactggcaat gggcgcttgt
     4081 tattgatctt cacatcgatt actacaggac gatctgattt tgcagctgca tcaaaggcaa
     4141 atcgaagttg cgagtattct gtgactgtaa agctatttgc cccgagcgct tctccaactt
     4201 taccgaaatc tgcatcttct aaaaatacac caaatttctt ttgttccgta tcttcttgtt
     4261 ctgcttcgat gaaaccaaaa gaatcattcg aaaagacaac attaataatt ggaagctgat
     4321 atttgacttg tgtaatgata tcttgcatga ccatagcaaa cccgccatca ccacttaagg
     4381 taaatacttg tttttctggg tagcttagtt gtgcggcaat tcctcctggc acaccattac
     4441 ccatagttgc gaaccatcca gatgtcgtat gtttttgttt accgttcata ttcaataaac
     4501 gaatagaatg gatagtcgtg ttaccgacat ctgtgacgaa aatcgcatcg tcttcagcaa
     4561 tacgattgat ttctttatag acagcctctg gacgaatcgg ttcttcctct cgatcttcaa
     4621 aagatttcaa ccaatttacc catttttctt tattttcttg atttgctagg taccagctat
     4681 cagcagggcg agcatcccct aactcgacaa gtctttgcaa agctgtgttt gcatcaccga
     4741 ggatcgacaa agatgtttca tggcgtcgtc caaattttga cgcatcgata tcgatttgga
     4801 taaattcagc agttggatta aaaaatgcac gtccaaatgg gaagtcgcta ccgacaaata
     4861 agatcagatc ggcttctgcc aatgcttcat ttccgggttt agtagcgaca cgtccagcaa
     4921 aaccaagaaa gttttcataa tcatcaggaa taattccttt tgctagaacg gaagcgacaa
     4981 caggaactga aaaatgttct gagaattctt tgatttgcgg aaatccgttt cgcgttcctt
     5041 gaccaacata aagaactggt ttttttgctt tttcaagata aggcaaagca gctagcaaat
     5101 ctttttcttc tggtaatatc acacttgttt tgtgagtgtg agcggtggag aaaggttgtt
     5161 cgtcaatttc ttcaaaacca agatcaacag ggatagtcac gatagccacg cctttatgct
     5221 cataggcggc tttaatggct tcatctacta catgcggaag actttgtggt gtcatgactg
     5281 tacgattgta tacgctaaca tctgcgaaaa taggattttc atttagttcc tggaaagagt
     5341 tatagttcat ggatgtagaa gcaacttgtc ctaagaacgc tactagaggc acatggtcca
     5401 tctgtgcatc atacaaacca ttgatcaaat gtgtcgcacc aggtcctgca gagccgaaga
     5461 cagcgccgac tttccccgtt aattttgcat ctgcagcggc tgcgagtgcg ccgacttctt
     5521 catgacgaac ttgaatgtag ttgatttcgg ttttttcttg atatagcgca tccataattg
     5581 aattgaaaga tcctccggga atgccgtaaa tatggtcgat accccagctt tcaagaactt
     5641 ttaccattgc gattcctgca ttgatttttg acatggtgtt tcactccttt agcaatgatg
     5701 atacataaat tttttgtatc tagagtatag aactttttat cgaaaatccc aactaattcg
     5761 tcttttcaca aaaatgtagt catgtttttg ggaatatgct agagtagtac atagatagat
     5821 tcaagagagg ggaatggcat ggttgaatgg ctattgagac ttgaaccttg gcagcaagca
     5881 cttgctggga cagcttttac ttattttatg acagctcttg gtgccgggtt ggtttttttc
     5941 tttaaagaaa tcaaaaaaga agtgctaaac ttgatgttag gatttgcttc tggtgtcatg
     6001 attgctgcaa gtttttggtc tttgttagat ccggctatta caaaagcaga agaaaatgga
     6061 gatattgctt ggctagttgt aagtattggg tttggtttag gcggtgtgtt tttatatatg
     6121 gcagataaaa cactgcccca tatgcatttt ggaccacaac atgaagcaga aggattgcct
     6181 acccatttaa aacggacgat cttgcttgtt ttttctatta cattgcataa cattcctgaa
     6241 ggtttagccg tgggggtagc attcggtgca gcagcaactg cggataatcc gacagccgct
     6301 atattagccg ctgtttcggt ggcgctaggt attggtatcc aaaacttccc agaaggagca
     6361 gctgtatcaa tcccgttgcg acaagaaggt ctgagtcgta aaaaggcctt tgtttacgga
     6421 caagcttctg gaatcgttga accaattgct ggagtgattg gagcgctact agtaacgaaa
     6481 gtggagctgc tgcttcctta tgcacttgct tttgccgcag gagcaatgat ttatgtcgtg
     6541 gtggaagaat tgatccctga agcacagcag acattgtcca gcaaacgtca ttttgcagtt
     6601 tttggcgtca tgagtggatt tatcattatg atgattttgg atgtggcatt aggataaata
     6661 aaaaaaatat atatgatata tcaaataggg ccgcgaactt atatcgcgac cctatttttt
     6721 tagtaagtta tatcataatg aagtgaatgg tatcatacat taataatagt tttttctatg
     6781 tgtatttcct ttgatccagc gaacaagcca gatgataagt gcgattggga ttagaacttt
     6841 gaccgcaaac cataaaagcc cgcttaggac actgacaaaa agactgatga cgaagctagc
     6901 tgcaattagc actaaaagaa ccatgaagat atttaagaac atattttttt gacgcataca
     6961 aaaccctcct cttgttcatt ctttactata gaaaaagtat aacagggatt gtttaaaaac
     7021 acctccaact gaagactgat ttttacttca tctacttaaa tgatcaaaca attgcttttt
     7081 ctctggttta tcatctaaaa tagagcggaa aaggaggaga atatcatgcc taaaaatcgt
     7141 cttgaagcat ttacagatgc tgtaatcgcg attatcatga ctattttagt tttagaactt
     7201 catgaaccta acggagacac gtgggaggcg ctcgctgcat taggttaccg attttttatc
     7261 tatattgtca gttttatcat tttagcgatt tattggaaca atcatcatca tttatttcaa
     7321 gttgtacata agataaatgg acgggtctta tgggcgaaca gtttctttgt ttttgcgctt
     7381 tcgctttttc cgtttgcaac ttcatggatc agtgagtaca tcaatagcct ggctccagaa
     7441 atgacttatg gttttgttac cttgaccgct aatattgcgt attatatctt ggcaaaagaa
     7501 cttgtcaaag caaataaagg ccacgagaaa gcattgaagg cgatgttgaa taattacaag
     7561 aaatcgtatc tatcgattgg tttgaatgtg ttaggaattg tcttagggta tttagtagcg
     7621 ccagtagccg tgctgatcgc aaacatcgtg attctagctg cttggatcat cccaagtcgt
     7681 accatcgaat cacaatatcg gtaaaatgat ccgtgtttaa aatttaagag aaaaatatag
     7741 aaaaaataaa taaacgatca aatatccgag tgatactagt attcaaagag ggatgcagat
     7801 ggatcaaaaa agaattcatg taggactgat tactagtttt gtcacgatgc ttatttattt
     7861 ggatcaaggt catttgactt ggacagtagt tggtgtcgta ggatttttga cattattcac
     7921 agcaataaaa aaataaacat ctttatgtaa tctg
//
LOCUS       DAKBVG010000123         7688 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000123 DAKBVG010000000
VERSION     DAKBVG010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7688
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.123"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(22..2193)
                     /gene="copB"
                     /locus_tag="OUD09_001891"
     CDS             complement(22..2193)
                     /gene="copB"
                     /locus_tag="OUD09_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HCT9693153.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(2213..4399)
                     /locus_tag="OUD09_001892"
     CDS             complement(2213..4399)
                     /locus_tag="OUD09_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HCT9693154.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDALFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(4399..4608)
                     /gene="copZ"
                     /locus_tag="OUD09_001893"
     CDS             complement(4399..4608)
                     /gene="copZ"
                     /locus_tag="OUD09_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HCT9693155.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(4621..5061)
                     /locus_tag="OUD09_001894"
     CDS             complement(4621..5061)
                     /locus_tag="OUD09_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HCT9693156.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(5135..5674)
                     /locus_tag="OUD09_001895"
     CDS             complement(5135..5674)
                     /locus_tag="OUD09_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HCT9693157.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITGFPLLPLDLLWHTTDYSIQA
                     KKWFIQQQQSFMETNHSWIIEGNYTSTLGKRIKEADKIIWLQVPRCRAIYRVILRSLK
                     RKINKKTRPDMPETFSERFDKEYLEFLSFIWHFEETHEPEIQQLIQENHAQNKLIILK
                     TRGEKQEFLTKLKIESKNN"
     gene            complement(6168..7634)
                     /locus_tag="OUD09_001896"
     CDS             complement(6168..7634)
                     /locus_tag="OUD09_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCT9693158.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         2075 a         1696 c         1351 g         2566 t
ORIGIN      
        1 tgtcacaacc ttttttcatt attatttgat tttcaatgtc aaagcattta gagctacaac
       61 taccgtactt aacgacatca agacagcacc aactgctgga cttaatatga taccaattgg
      121 cgcaagaatt ccggctgcta gcggaattgc aagaatattg taacctgccc cccaccagag
      181 gttctggatc attttctttc gcgtttgctt ggctagatca agaaaatgca aaatatcttt
      241 tggatcgctg tttgtcaata cgacatccgc agaatcgatt gcgatatctg tccctgcacc
      301 gattgccatc ccgattgaag cacgggctaa acttggcgca tcattgatcc catcacccac
      361 catgattact tttttccctt ggtcaaggta tttctggaca acagcttctt tatcatctgg
      421 taataaaccg ccataatatt cttcgatccc taaataatct gctactgctt gggcagcgtt
      481 tttattgtca ccagtaagca tgacaggagt gatttttctt tctttcaatg ttcggataaa
      541 ttcttttgct tctgttttta ctacgtcacc taaagcaacg attgcagcta gatgattttc
      601 taaaatcagg aagctgatgg tatttccttg ttcttggtaa ggtttcaata atgcttgttc
      661 tacgtttaat ccgagacgct caacttcttt ttcattaact attttcactt gttgtttttt
      721 gactgttgcc tctagcccga ctcctgaaag attttttaga ttttcagctt gataaggctt
      781 gatttcatgt tctttcaaat aattcataat gccaacagcc aatggatgat tggcattcgc
      841 ttccaaggct cctagatatt gaaggatttc atttttattg aacgcatcat caagtacttc
      901 gacacccgtt acagtaaatt ttccttctgt tagtgttcct gttttgtcca gcatgatcac
      961 atctagatca ttggcttgct ccagtgcatt gcggtttttc aaaagtaatc cgtttttagc
     1021 tgctattgat gtggagcgag caacaactaa tgggatagct aaacctagag catgaggaca
     1081 ggcgatgata aagactgtta ccatacgttc taatgcatta ggaagatctg tcaagaacaa
     1141 ccaggcgatg aatgccagga tacccacaat taatgcgaca taaaacagcc attttgccac
     1201 tttgtcagat aatgactcta gcttggattt ttctccttgt gcttttcgca ccatttccat
     1261 aaccttcgct agataaccat tttcaccagt tccagttact tcgatttcga tcgttccatc
     1321 cccattgata gaaccgccaa tcactttgtc attggcttgt tttttgattc ctcttgattc
     1381 gccagtcaca gctgattcgt caacaattgt ttcacctttc aagattacac catctgttgg
     1441 cattttatca ccagaccgga cgatcaaacg atcaccttcg tttacttctt tcaatgaaac
     1501 tgtctcttct tttccatctt tcgtcaagcg tttcactgat tctggcagta attctgctaa
     1561 tttttgtaaa gcattgctgg cattcgacac agcattcatt tcaatccagt gacctagtag
     1621 cataataacg atcaaagtag ccaattccca gaagaaatcc atgacgtgtg tatgtggatt
     1681 gagcagattt gcgataaacg aatagacact ataaatatat gcaactgtaa tccccatcgc
     1741 gatcagtgtc atcatagcag gacttttttg tttcaattcc atcttagctc cacttaaaaa
     1801 tggctgaccg ccatagataa atagtatagt tgctaagatc aatacgagcc aatcagaacc
     1861 aggaaacgtt acttgaaatg gaaattccat tcccatcatt ggtgaaaaaa gaatgattgg
     1921 gatagctagg ataagcgata gccaaaattt ttgtttgaaa ttccccatgt gcatggaatg
     1981 atccatgccg ccgtgttcgt gatccatgtg catcgaatgg tccatttgtt tgctgtttgc
     2041 tatatctcca tggttatgtc ccatgtgcat ggaatgatcc atctgactat ggtcatgttt
     2101 catctccatc tgtttttcat gagattctgt actttctttg tttactactt cttttttatg
     2161 ctcatgtttt ttctccatat cgttcccatt catttttact ccatcccttc tcttactttt
     2221 tcatgtgtcg atttaagctc agggagttca acaatacact gacggaacta aacgccatcg
     2281 ctcctcctgc aatgattgga ttcaaaaagc ctagtgcggc aaaaggaata ccaatcgtat
     2341 tatagacaaa agcccagaaa agattttgtt tgatttttct caatgtcaac ctagacaact
     2401 cgattgtttg tgcaatggat gccaaactgc tattcatcag cgtcacatcg gctgtttcca
     2461 tcgcaatgtc cgttccactt cccattgcca taccgacatc agctaatgct aaggccggtg
     2521 catcattgat tccatcgcct gccataccga cttttttgcc atctttctga agtttttcta
     2581 cgtaggctgc tttatcctct ggaagtactt cagcaaagat atgctttgga tcaataccaa
     2641 cttgtttacc aatcgtttcg gctgccagtt tgttgtctcc tgtcagcata aaaacatcta
     2701 atcctttatt ctggagctgc ttgattgctt gtttggcttc tagtttgatt tgatcagcca
     2761 cggcaatcaa cccgatcact tgcttttcat cagctaaaaa catgactgtt tttccctgct
     2821 gttctaattc taacgcgtgt tcttggtatt catcaaatga taggttcaat tcaattaatc
     2881 gtttacgtgt acccgcaaaa tagcgtactc cattaatcgt accgctgatc ccagcacctg
     2941 ggtgggctgt aaaatcaatc atatcgtatg ttgctgcttc ttgcagtttt ccgtattcca
     3001 cgattgcttt acctagtgga tgttcagaag cttgttctaa agtataaaaa atagatagga
     3061 cttctttaga accgactaga tctgtaactt tgggctttcc ttctgtaatc gttccagttt
     3121 tatccaaaac aatgctgtct aagtgagcag ctgcttctaa tgcttctccg cctttaatta
     3181 aaattccatt tcgtgcacca acacccgttc ctaccataat agctgttgga gtcgctaaac
     3241 ctaatgcaca tggacacgca atgactaata cagagacact atggatcaat gcgagttgcc
     3301 aatcaccagt gatcaaacct gtagctatga gcgtgataaa cgcgatgact aatacgatcg
     3361 gaacaaaaat ccctgagatc cgatctgcga ttttttggat tggtgctttg ctcccttgag
     3421 catcttccac catttgaatg atttgcgcta atactgtctg cttgccgact tgtgtgactt
     3481 ccatgtgtag cagaccattc gtattgattg tcccgccaaa aagcgcgtca tccggattct
     3541 tttctacagg aagattttca ccagtaagca tactttcatc tatcgcactg cttcctgaaa
     3601 taattcgacc atctgctgga atctgctcac caggacggat gactagttga tcaccaacga
     3661 ctacttcttc aatcgctaac gtttcttctt tcccgtttct tatcacttgg gcggtttttg
     3721 tttgaagaga catcaattgt ttgattgcat tacctgtttt cgtttttgct gtatgttcca
     3781 aatatttgcc gagcaaaatc agcgtgatga tcatactgct gctttcaaaa taaagatctt
     3841 gggggtgtcc acggaaaaat ccgttgtaaa tgctcaatgc aaaggcagcc gatgttccaa
     3901 ttgctaccaa tacatccata tttggtgctt tcgtcttgat tgcatggtac gcacctttat
     3961 aaaaacgagc accgacataa aattggacag gagcagacag cactaactgg acgattggaa
     4021 aatgaaaaaa gtgaacaatc gccgcatggc ttcctaacat catagcaatc attgacaaca
     4081 tcaacggcaa agtcaaaacc gtactcaata taagatcgaa tagcattttt ttcaaatacg
     4141 cttgcttttc ttctgcaatt ttttgtttgt gggcttcatc gtacaaaatt gcaccatagc
     4201 cgatgttttc tacgctttgg attaattttt cagtggtcgt cccttcatat ttgacacttg
     4261 ctttttcagt ggccaagttg actgtggcat tgaccacacc aggttgtccg ttcagctctt
     4321 tttcgattct agcagaacaa tttgcacaag tcatacctgt aatgacgaac gtatccattt
     4381 tggtttctgc tgttgccatc aaatcacctc tgctttataa cccaatccat tgattgcttg
     4441 acaaatcttt tcggattgaa cttttgtttc atcaaacttg accgtaccgt ttgctttttt
     4501 caaatttact ttcacttttt gcacgccgtc aagtgcgctt actgtttctt ctactcttgc
     4561 tgcacaatga ttgcagctca taccttcgat tgaaaattgt tgtttcattt tttcttcctc
     4621 ctattgatgt tgtttacatt cacattgacc tgggatacag ttacattcga tcgtatctac
     4681 tggttgcttt tgttccagtt gtttcaaaat ctcattgata tcttcttgag tcaaagtggc
     4741 ttcttctact agatccgcaa tcgtctctcc aatccttttt gcacagatat gggaaaaaag
     4801 attttccgtc gcactgcgaa ctgtttccat ctccgagaca gctggatgat aaatgaattt
     4861 ttttccttct tgttctgtcc agatcacttc tttttttacc aaacgtccca gcaaagtttt
     4921 gatcgttgct ggtttccaat ccatcgaatc agctaagatc tgcgtgattt cttgggctgt
     4981 cgtctgtcca agtgtccaaa taacacgcat gatctcccac tctgaatcac tgatctttac
     5041 tggcgttttg actttttcca tttatttcac acctttcgtt tacaaatgta atcaataact
     5101 tatggataca gattacactt gtaaacaaaa actgtcaatt atttttgctc tctattttca
     5161 atttagttaa aaattcttgt ttttcccctc ttgttttaag gataatcagt ttgttttgtg
     5221 catggttttc ttggatcagc tgctgtattt cgggttcatg ggtttcttca aaatgccaga
     5281 tgaaagacag aaattccaga tattccttat cgaatctttc tgaaaatgtc tcaggcatat
     5341 ctggtcgggt cttcttgttt atctttcttt tgagggaacg taaaatcact cgataaatcg
     5401 cccggcaacg cggaacttgt agccaaatga tcttatctgc ttctttaatc ctctttccta
     5461 gtgtagaagt atagtttcct tcaatgatcc atgaatgatt agtctccata aatgattgct
     5521 gttgttgtat gaaccacttt tttgcttgga tgctgtaatc tgtcgtatgc cataaaaggt
     5581 ctagcggcag tagcggaaag ccggtgattt ctttgatctt ccttgcgagg gtcgattttc
     5641 ccgacccact ttgtccaatc acgatgatct tcattcttct ccctcacttc tcttatcatt
     5701 ttgatttgat cataccactt ataacgccgc aaaaaaacaa gggactgtga caaaagtcgt
     5761 gtcaaagtcc cttgtttcga ataaatggtg ttcaatcagc tgaccttcag cattagctca
     5821 aaaagcggca aaatatgagg agctattttt ccgtttgttt ctctaatact caggtcaaat
     5881 cgctgatttt acaacctctt ccttaaacgg tgttcaaaag cttgcatctg cggtaataag
     5941 cccagccaaa cgaaaaatat gaggggctat ttttgtttgg ctgttcttat tacttgatgc
     6001 aggacagctt tttcacaacc tctgattcac ggtgttccag aagtagcttc ttcgaaaaac
     6061 aagccgaaac ttcacaaaaa tttgagaagc aattttcgta cattccttct tatttcttga
     6121 agctgagacc ccttctgtca caacctcttg taacaagtat ttctatttta aggctgaacc
     6181 actgtttctg gttcagtgat ttctttgttt ttgtctcgac gagcaattgc tggcaagatc
     6241 atacctaata agatcaagac gattggtgtg atgatattcg tgaataattg gaaccaccat
     6301 gctttaggat cagcagtata atctactttt ggcaccattc ctaggataca tgcaaacgca
     6361 gtgaaggcaa agcaccaagc accaaagata aaaccgattt ttggattttt aacaaattta
     6421 tattcagaat tgaatttctt ataagcttta ttcaacatca tatatgccaa gaacacccat
     6481 aggtaacgca ttggcatgac aactgagttc aagttcgtca accattttac taattcattc
     6541 atatctttga taccaaagat cggcaacaaa ataatgattc ctactagtat acctgtcaga
     6601 agataaccat tcaccaatgt acctttacga ttacgtttac gtagccagtt tggtacaaat
     6661 tctggatccg catctgctaa caagattttt agtggtgcat caattgaaaa ggctaatgcg
     6721 gagatttgtc caatcatgtt tgtcaatgcg taaatgatca tcaatgagtt accgatacca
     6781 tagtaattac ctaaaatttg aaaagcactg tatgccccat ttgccatcaa gtctttagga
     6841 atattatcgc tagcaaacag catacccatt gcgactgagc ctaatacagc acaaatccct
     6901 accattgctg ctaagaagat catcccttta ggaaattcct ttgcaggatt tcttgtagag
     6961 ttgacataag gggatatttt ttccgctcct ccaactgcaa atactagcat cgaaacggtt
     7021 gtgaaataac tgaaatcaaa tgttggaata tatgttttca cttgattcat atgtggtgtg
     7081 gcaaattcca tcgttggatt gatgaacggt gccgttacag ctaaaagaat aaataagatc
     7141 gacatgacga acatcgctgt tccggccagt ccaccaatca ctttcaaagt agaaagtcct
     7201 tttgttgata aatataaaaa ggcaagaaat ataataagtg aaataactgc taccatttgt
     7261 actgacatcg tatttacgat atttccattt ccttggactg cccaaccgcc agcaatcagg
     7321 attgcttgag gcttttgtgc caaataagga atatggacga cccagtaagt ccaagcggca
     7381 tagtatgcca gtcgtttatt agatgtcgta ttttcgaccc atgaactgac gcctccttta
     7441 ctatccttga aggctgatcc taattgtcca acgatcaatg cgtatggaat aaaataaatg
     7501 gccaaaatca atagccaaga ggtgacaact gatactcctt gctgagcata gttattaact
     7561 acattcccca tgccccaaac catgttaaat gcaattaacg caacagtaaa ccagcgtaat
     7621 tgtttgttgt tcattcgttc tctcctttat gataaaaatt tgttatccag atgtaatcat
     7681 attcaatt
//
LOCUS       DAKBVG010000124         7639 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000124 DAKBVG010000000
VERSION     DAKBVG010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.124"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            <1..286
                     /locus_tag="OUD09_001897"
     CDS             <1..286
                     /locus_tag="OUD09_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCT9693159.1"
                     /translation="LSWKVNQSGNYQSQNTPLTKQGNRYFRYYLVEAANSVKNYLPEY
                     KAFYQSKYKEVPKHQHKRALVLTARKFTRLVDTLLRKHQLYTPPRSVIEK"
     gene            complement(474..1298)
                     /gene="iolB"
                     /locus_tag="OUD09_001898"
     CDS             complement(474..1298)
                     /gene="iolB"
                     /locus_tag="OUD09_001898"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HCT9693160.1"
                     /translation="MEKTLKHQPLNKELAPGVKQIQNIRQSNSALSYIEFQILELEKG
                     ASYKETGSQQEVCIVILTGKIKVKAADTWYEDLGTRESVFEKIPTDSIYLPAEHSFEL
                     VATTDNVRLALCYSPAKKALPISVIRAEDNTVEERGLYQNKRLVHNILPDNVDTASSL
                     LVVEVYTEGGNFSSYPPHKHDQENLPHESFLEETYYHELDPEQGFVFQRVYTEDRSLD
                     ETMTVEHQNVVEVPKGYHPVGVPDGYTSYYLNVMAGPERIWKFHNEKKHEWILQRK"
     gene            complement(1337..3253)
                     /gene="iolD"
                     /locus_tag="OUD09_001899"
     CDS             complement(1337..3253)
                     /gene="iolD"
                     /locus_tag="OUD09_001899"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002397168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HCT9693161.1"
                     /translation="MEKTVCLTTAQALLKFLNQQYISIDGKETPYVEGIFHVYGHGNV
                     LGIGQALELEPGHLKSYSGKNEQGMAHAATAFARQNLRKKIFAVSTSVGPGAANLLTA
                     AGTAFANNIPVLFLPADTFATRQPDPVLQQLEHASSVAFSTNDAFQAVSRYWDRINRP
                     EQLMSALLRAFEVMTNPATMGPATICLPQDVEAEAYAYPEEFFKKRVHYIDRRIPTAR
                     ELAEASQRIKASKRPVIIVGGGCKYSEAGEILEQISRDCQIPLVETHAGKSTVSWDFK
                     NNLGGMGILGTSAANKAVQQADLIIGIGTRYTDFTSASKSIFNFEQAKMMNINVSRQQ
                     AAKLEAFQVVGDAKATLEQLAPMIEGYQTAYGEKIDELKEEWISERNRLKAAHFKRKD
                     FTPEIKDHFSQEILNEYADALATNLTQTEVFIHLNDFVDEDAIVIGSAGSLPGDMQRL
                     WNPVKENTYHLEYGYSCMGYEIAGAMGVRMARPEQEVYALVGDGSFLMLHSELVSSLQ
                     YDKKINIVLFDNSGFGCINNLQMDNGGASQGTEFRNTKDEIMNIDYAKVAEGYGAKTY
                     RVSSIAELQAAVEDAKKQTRSTLIEIKVLPKTMTEGYDGSWWNVGISEVSGKAAMQEA
                     YKAKQAKLATIRKY"
     gene            complement(3264..4298)
                     /gene="iolC"
                     /locus_tag="OUD09_001900"
     CDS             complement(3264..4298)
                     /gene="iolC"
                     /locus_tag="OUD09_001900"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HCT9693162.1"
                     /translation="MSYIKFDQSKEFDLLLLGRVAIDFNPAYNDQVKEEFKPLKKVHM
                     FEKFVGGSPANIAVGVTKHGMKAGFIGTVSDDQFGEYVIDYFNEQGIDTSQISKAQHG
                     EKLGLTFTEMLSSKESNILMYRNQAADLQLSVEDIDEDYLKRSKALLISGTALAESPS
                     REAVIKAVMLAKRNNVKIIFDIDYRAYNWKNNDDISIYYSMIAKESDIIMGSREEFDL
                     TEKLIQPNMSDNESAAFWHSCGAEIVVIKHGMKGSTAYTADQQQFSIKPFPVEARKGF
                     GGGDGYGSGFLYGLFEGWEIIDCLEFGSAEASMMVKSNNCSDSLPTTQEVKDFITLEK
                     EIYGEMIARI"
     gene            complement(4455..5471)
                     /locus_tag="OUD09_001901"
     CDS             complement(4455..5471)
                     /locus_tag="OUD09_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016633586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCT9693163.1"
                     /translation="MAGTIKEIAEKAGVSRGTVDRALNDRGRINPEVAERIRAIAKEM
                     NYVPKKKKVIKETTLKLGIVTQLAKSSFMIPIRSGLESIIGDLKKRNIDCFLEEVDGV
                     DEAAQLQALDRLVELGVKGIAIMPVDSDGIREKINQLTDQGILIITFNSDILGTNRQC
                     FVGMDNFKSGKTAAGLLGMLTKGKGKVLAITGYFGNSVNSLRVAGFVEELKESFPELE
                     LTGVQSSFDSSAEVEKILSNTLENYSNLDGVVVFSGGQAGIARALEKIDESKRPFVII
                     YDLTKNNIEMLKNDQVDFLIDQDGFVQGYRSLLMLANQLQNNKTVTKDALFTDIIIKT
                     KYNI"
     gene            complement(5573..5770)
                     /locus_tag="OUD09_001902"
     CDS             complement(5573..5770)
                     /locus_tag="OUD09_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693164.1"
                     /translation="MKFICLSCGEKESIPMDVVKFLDDADIANDPNNPPQFTCEKCGK
                     EMYPEYYRNALGIEFKISDVQ"
     gene            complement(5742..7061)
                     /locus_tag="OUD09_001903"
     CDS             complement(5742..7061)
                     /locus_tag="OUD09_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016372324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HCT9693165.1"
                     /translation="MKANILEAIFFDEHHNWDNFVEKYGRKIRLVVKREVNKFRYCGD
                     IQKGYRLFVCEGCHQTKFVPLKCRGKFCPTCATGESQRWSAVVANDMFKVTHRHVIFT
                     IDEGLREIFMMENYRDSLLKGLMDEAAKIILAYFEKRKVKAGIVATIHTFGSKLEFNP
                     HVHMVVTMGGLTRDGSWEEYDYLPFSMLRKYWKNAVLKLIRRTLGEWDKQRVQSRLQA
                     AYKNNGEGFYVNAPKRSRTNLKSLLQYISRYMKRGPIALSRILMYDGDTVMFNYMDKR
                     TNTKETMTMSIDEFIAALIRHIQNKNFKTIRRYGIYSRRIKTLMKKVMKEYQKRVKKL
                     FINLNEALRPKSWEERITEEFGENPLECSCCGEYYEFVGMSVRKNGRLVIKYAKDNHA
                     KRFMREENRKIDQKEFRQQYEEEKEKTFKAIRWDWERQREIYMSKLW"
BASE COUNT         2065 a         1833 c         1293 g         2448 t
ORIGIN      
        1 attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac ctctgacaaa
       61 acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg taaaaaatta
      121 tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa agcatcaaca
      181 caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata cgctactacg
      241 taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat ccgttattga
      301 cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag tttcgtgcgc
      361 ttcaaaacat attactcaat aaagaaaatc ttaaatttca tcttgactta tcaccattag
      421 actaaaaacc taactaaacc taactaaact ttatgtatct aacagcttaa taactacttg
      481 cgctgcaaga tccattcgtg tttcttttca ttgtggaatt tccaaattcg ctcaggtccc
      541 gccatcacat ttaaatagta ggaagtatac ccgtcaggta ccccaactgg atgatagccc
      601 ttaggaactt cgacgacatt ttgatgctct accgtcatcg tttcatctag tgaacgatct
      661 tccgtgtaga cccgctggaa aacaaatccc tgctctggat caagctcatg gtaataagtt
      721 tcctctaaaa aagactcatg tggcagattc tcttgatcgt gcttatgagg aggatagctg
      781 gaaaaattac cgccctcggt ataaacttcg acgaccagca ggctgctggc agtatcgaca
      841 ttatccggta aaatgttgtg aacaagtcgt ttattctgat agagtccacg ttcttcgacg
      901 gtattatcct ccgcgcgaat cacacttatc ggcaaagctt ttttcgctgg tgaatagcaa
      961 agcgccaatc ggacattgtc tgtagttgcc acaagttcaa acgaatgctc cgccggaaga
     1021 taaatactgt cggttggaat tttttcaaaa acagattctc gtgttcctaa atcttcatac
     1081 caagtgtctg ctgctttaac cttaattttt ccagtcaaaa tgacgataca gacctcctgt
     1141 tgactgccgg tttccttgta agaagctccc ttttccagct ccaaaatctg aaattcgata
     1201 taactcaaag cagaattaga ctgccgaata ttttggattt gttttactcc aggtgccagt
     1261 tctttgttta atggttgatg cttcaatgtc ttttccaatc aatccactcc ttattttcaa
     1321 tcacaaaaag caatatttaa tactttctga tagtggcaag tttcgcttgt ttggctttgt
     1381 aagcctcctg catggctgct ttacccgata cctctgagat accgacgttc caccagctgc
     1441 cgtcgtagcc ttctgtcatg gttttaggaa gtacctttat ctcaatcaac gtcgatcgcg
     1501 tttgcttctt cgcatcctcg accgctgctt gtaattctgc aatcgagctt acacgatacg
     1561 tttttgcacc atagccttca gcaactttgg cgtaatcgat gttcatgatc tcgtccttgg
     1621 tatttctgaa ttcggttcct tggctagctc cgccattatc catctgtaaa ttattgatac
     1681 agccgaaacc agaattatcg aaaagtacaa tgttgatttt tttatcatat tgcaaactag
     1741 aaaccagctc gctatgcaac atcaagaaac tgccatcgcc tactaaggca tagacctctt
     1801 gttctggtcg agccattctt acgcccatcg caccagcgat ttcatagccc atacaagaat
     1861 aaccgtattc caaatgatag gtattctcct tcacagggtt ccataagcgc tgcatatccc
     1921 ccggcaatga accggctgaa ccgatcacga tcgcatcctc atcaacaaaa tcatttaaat
     1981 gaatgaagac ctctgtttgc gtcaaattcg tagccaacgc atcagcatat tcattcaaga
     2041 tttcttgaga aaaatgatct ttgatttctg gcgtaaaatc tttacgctta aaatgcgcgg
     2101 cttttaaccg atttcgctca ctgatccatt cttccttcag ttcatcgatc ttctcgccat
     2161 aggcggtttg gtaaccttcg atcattgggg caagctgctc caaggtcgct ttcgcatcac
     2221 caaccacttg aaaggcttcc agcttagcgg cctgttgtct gcttacgtta atattcatca
     2281 ttttcgcttg ttcaaaatta aaaatacttt tagaagctga ggtaaaatca gtgtagcgtg
     2341 tgccgatccc tatgatcaga tccgcttgct gcacagcttt attagcagcc gaagttccca
     2401 aaatccccat tccgccgaga ttgtttttaa aatcccacga aacagtcgat ttacctgcgt
     2461 gcgtttcaac tagcggaatc tgacaatctc ttgagatttg ctcaagaatc tcaccagcct
     2521 ccgaatattt gcagccgcct cctacgatga tgacaggtcg tttactggcc tttattcgtt
     2581 gactcgcttc agcaagctca cgagcggtcg gaatcctgcg atcaatataa tgtactcttt
     2641 ttttgaaaaa ttcctccgga taggcatagg cttctgcctc cacgtcctgc ggcaaacaaa
     2701 tcgttgccgg acccatcgtt gccgggttcg tcatgacttc aaaggcccgc aataacgcgg
     2761 acatcaactg ctctggtcga ttgatgcggt cccagtagcg agaaaccgct tgaaatgcat
     2821 catttgtcga aaaggctaca ctgcttgcat gttccagctg ctgcaagacc ggatcaggct
     2881 gacgtgtagc aaacgtatcc gccggtaaaa aaagtacggg gatattattt gcgaatgccg
     2941 tacccgctgc tgttaacaga ttggccgcgc ccggtccaac agatgttgat acggcaaaaa
     3001 ttttcttgcg taaattctgt ctggcaaagg cagtagccgc atgggccatt ccctgctcat
     3061 tttttccgct ataacttttc aaatgtccgg gctctaattc cagcgcctga ccgatcccca
     3121 aaacatttcc gtgaccatag acatgaaaga taccttcaac ataaggcgtt tctttgccgt
     3181 cgatgctgat gtattgttga ttcaaaaatt ttagtaatgc ttgtgctgtt gttaaacaaa
     3241 ccgtcttttc catttttttc accttaaatc cgagcgatca tttcgccgta tatttctttc
     3301 tccaatgtaa taaaatcttt tacctcctga gtcgtcggta aagaatctga acagttattg
     3361 cttttgacca tcattgatgc ttccgctgag ccaaactcta aacaatcgat gatctcccag
     3421 ccttcgaata aaccgtataa gaaacccgag ccgtaaccat ctccgccgcc aaagcctttg
     3481 cgtgcttcca ccggaaacgg cttgattgaa aactgctgct gatcggccgt ataagcagtc
     3541 gatcctttca ttccatgttt gattacgaca atctcagcac cacaactgtg ccaaaaagca
     3601 gcgctttcat tatctgacat gttaggctgg atcaacttct cagttagatc gaactcttct
     3661 cttgaaccca tgataatgtc tgattcctta gcaatcatcg aatagtaaat tgaaatatca
     3721 tcgttgttct tccagttata agcacggtaa tcaatatcaa aaataatttt gacattattc
     3781 cttttcgcca gcatcactgc cttgattacg gcttctctgg acgggctctc tgctagtgca
     3841 gttcctgaga ttaatagtgc ttttgaccgt ttcaagtagt cctcatcaat atcctcaacg
     3901 ctaagctgca ggtctgcggc ttgatttcga tacatcaaaa tattactttc ctttgatgag
     3961 agcatttcgg taaaagtcag ccccagcttt tcaccatgct gcgctttgct gatctgagac
     4021 gtatcgatgc cttgttcatt gaaataatcg atcacatatt cgccaaactg atcgtccgag
     4081 actgttccga taaaaccagc tttcatgcca tgtttagtaa cacccacagc aatattggca
     4141 ggagatccgc ccacaaattt ttcaaacata tggacctttt tcaatggttt aaactcttct
     4201 ttgacttgat cattgtaggc aggattaaaa tcaatagcga ctcttcctaa aagcagcaag
     4261 tcaaattctt ttgattggtc aaactttata taactcaata tatccacctc tttatccgtt
     4321 cttctatttc tgaggcaagt atagcacgcc aaaacaaagc gtgcaatatt tattttagaa
     4381 cgcaatattg cgtgctgttt gaaaatgacg gcaaaaaaaa cgtgaatcgc cttacatcac
     4441 gattcacgcc tagattaaat attgtatttt gtcttaataa ttatatcagt aaatagcgca
     4501 tcttttgtaa ccgttttatt attttgcagc tgattcgcta acatcagtaa tgaacgatag
     4561 ccttgaacaa atccatcttg atcgattaga aaatcaacct gatcgttttt cagcatctcg
     4621 atattgttct tagtcaaatc ataaatgatt acaaagggcc gcttgctttc atcgattttt
     4681 tccaacgctc gggcaattcc cgcttgtccg ccagaaaaga cgactacgcc atctaagttt
     4741 gaataattct ccaaggtgtt gcttaaaatc ttttcaacct cagcactcga atcaaagctg
     4801 ctctgaacac ccgtcagctc cagttcagga aacgactctt tcaattcttc aacaaagcct
     4861 gccacccgta agctgttgac gctatttcca aagtagccgg taatcgctag aaccttgccc
     4921 tttccttttg tcagcatacc taacaatccc gcagccgttt ttccgctttt gaagttgtcc
     4981 atcccaacaa aacactgtcg attcgtacca agaatatccg aattaaaggt aatgatgagg
     5041 atgccttgat cggtcaattg attaattttt tctctgatac catcactatc tactggcatg
     5101 atcgcgatcc cttttactcc aagctccacc aatcgatcca acgcctgcag ctgggccgct
     5161 tcatcgaccc cgtctacctc ttctaaaaaa caatcaatgt ttcgcttttt taaatccccg
     5221 ataatagatt ccagacctga tcggatcgga atcataaaag aagatttcgc caattgagtg
     5281 acaatcccca gcttgagcgt tgtctctttt atgaccttct ttttctttgg aacatagttc
     5341 atttccttgg cgattgctct gattctttct gcaacctcag gattgatcct tcctcgatcg
     5401 ttcaatgccc gatccactgt cccccttgac actcctgctt tctcagctat ttctttaatt
     5461 gtacccgcca tcttgcctct ccttttttcc tattgtgttt attttatcac aaaacttaag
     5521 aaaaccaccg gagaatgaat tctccggtgg actttttgta tgattaacct tcttattgaa
     5581 catcactgat cttaaactct attccaaggg cgtttctata atattctgga tacatctcct
     5641 tcccacattt ttcacaggta aattggggag gattgttcgg atcatttgca atatctgcat
     5701 catctaaaaa ttttactaca tccattggga ttgattcttt ttcaccacaa cttagacata
     5761 taaatttcac gttggcgttc ccaatcccag cgaattgctt taaaagtttt ttctttttct
     5821 tcttcatact gttgcctgaa ttctttttgg tcaatttttc gattctcctc tcgcataaac
     5881 cgctttgcat ggttatcttt cgcatattta ataactaacc gtccattttt gcgcacactc
     5941 atcccaacga attcatagta ctcaccgcaa caagaacact ctaagggatt ttcaccaaat
     6001 tcttcggtta ttctttcttc ccaggatttt ggtcttaagg cctcattcag attgataaac
     6061 agctttttta ctcgtttctg atactccttc atgacctttt tcattaatgt tttgattcta
     6121 cgactataga ttccatagcg acgaatcgtt ttgaaattct tattttgaat atggcgaatc
     6181 agtgcagcta tgaattcgtc tatactcatg gtcatcgtct ctttagtatt cgttcgtttg
     6241 tccatgtagt tgaacatgac agtgtcgcca tcatacatta agattcggct taatgcgatc
     6301 gggccacgct tcatataacg actgatgtat tggagtagtg actttagatt tgtcctgctc
     6361 cttttaggag catttacgta aaacccttct ccattgttct tgtacgcagc ttgtaatctt
     6421 gattgaactc tttgcttgtc ccactcacct aacgttcgtc taattagttt caatacagca
     6481 tttttccagt attttcttaa catagaaaaa ggtaaataat cgtattcttc ccaacttcca
     6541 tctcttgtta agccacccat agtaacaacc atatgaacat gcggattaaa ttctagtttt
     6601 gatccaaagg tgtggattgt agctactata cctgccttca cctttctttt ctcaaaatag
     6661 gcaagaataa tttttgcggc ttcatccatt agtcctttca gtaaggaatc tcgatagttt
     6721 tccatcataa agatttcccg taacccctca tcgatcgtaa agattacgtg ccgatgtgtc
     6781 actttaaaca tatcattcgc cacaaccgct gaccaccttt ggctttcacc tgttgcacac
     6841 gttggacaga actttccacg acatttcaag ggaacaaact ttgtttgatg acatccctcg
     6901 cagacaaaca gtcggtatcc tttttgaata tctccacaat atctaaactt gtttacttcc
     6961 cgtttgacga ctagccgaat ctttcgcccg tatttctcaa caaagttatc ccaattatgg
     7021 tgttcgtcaa aaaagattgc ttcgagaata ttcgctttca tagtaatatt ttacttgttc
     7081 aaaaacaaaa tgaaaagggc taaaagactg gtagtgcagc ctttttagcc cttaactttt
     7141 tatactttct ttactgtcta aaaaatatgc ggcgcacaga acgcgccgca tatcggacta
     7201 agttttattc aaatttgtct tcgccaacac aatttgacga agagcctttt ctttcaggaa
     7261 gacttcggta atttttagga gttctaccta ttcttttaac cgtttattct ctttttctaa
     7321 gcgtgtaatt tcatttctgc gaatggattt catgacttcc tctaccgaga aaagtacgtt
     7381 atccatattt ttcaacttct tgaactcatc aatacacgct attagagcga gcttctagct
     7441 atagactaac ttctttaaca gaaaaccctt cttccgttac cagttttaca gcggtaattt
     7501 taaatatttc atcaaatgaa cgtcttggca taatccagat cttcgctttc tcttaatttg
     7561 gtgtcaattt tagtataacc cgtccaactt attttttaac taagtgcccg gtttttcttg
     7621 acaactccac ccaaaagtt
//
LOCUS       DAKBVG010000125         7599 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000125 DAKBVG010000000
VERSION     DAKBVG010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.125"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(<1..139)
                     /locus_tag="OUD09_001904"
     CDS             complement(<1..139)
                     /locus_tag="OUD09_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693166.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TF"
     gene            400..1260
                     /gene="ylqF"
                     /locus_tag="OUD09_001905"
     CDS             400..1260
                     /gene="ylqF"
                     /locus_tag="OUD09_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HCT9693167.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            1253..2020
                     /locus_tag="OUD09_001906"
     CDS             1253..2020
                     /locus_tag="OUD09_001906"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCT9693168.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            2076..2933
                     /gene="dprA"
                     /locus_tag="OUD09_001907"
     CDS             2076..2933
                     /gene="dprA"
                     /locus_tag="OUD09_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCT9693169.1"
                     /translation="MYQIEENLLKLAYAKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITTHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVVEAGYVIVSGCAKGIDTYGQQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHRFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            3055..5133
                     /gene="topA"
                     /locus_tag="OUD09_001908"
     CDS             3055..5133
                     /gene="topA"
                     /locus_tag="OUD09_001908"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCT9693170.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDIVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            5102..6478
                     /gene="trmFO"
                     /locus_tag="OUD09_001909"
     CDS             5102..6478
                     /gene="trmFO"
                     /locus_tag="OUD09_001909"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HCT9693171.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLDSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            6676..7581
                     /gene="xerC"
                     /locus_tag="OUD09_001910"
     CDS             6676..7581
                     /gene="xerC"
                     /locus_tag="OUD09_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCT9693172.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
BASE COUNT         2627 a         1340 c         1655 g         1977 t
ORIGIN      
        1 aaaaagtgtc atcccatacc attgaaccag cttcttcagt agaaaattct tcattttttt
       61 taaaacccag cttttcataa aaagcaacgg aacgattgac atcagcaaca ggaaaattaa
      121 caaaaaccat actattcatt tgtttcgtct cctttctgtt ttgttcgtct atcatctctt
      181 atttaggaaa cgttgtcaaa aatttagatt catatataga aaccaaaata aaaatttaca
      241 tcatcaaaaa agtgaagatg tgatagaatg aggactggtt cactttcact taactaatag
      301 atggaatttc tttataataa aaaagtgaat ttatattgaa aaagaaaaaa aacaagtaaa
      361 gccggggaat attccggtat gaaaggaatg gagagaacaa tgaccatcca atggtttccg
      421 ggacatatgg caaaagcccg acgagaagtt tctgaaaaaa tgaaatatgt ggacatcgta
      481 tttgaattgg tagacgcacg attaccttta tcttctcgta atccaatgct agatcagatc
      541 attcagcaaa aacctcgcct tgttttattg aataaagcgg atctggcaga tccacaacag
      601 acaaagctgt ggcaagaata ttttcgtaaa caaggccatc ttgcattagc tatcacggca
      661 caagaaagta aaggaattaa aggtctagta cctgcggcta aagaagcatt gaaagaaaaa
      721 cgggcaagag atgctgctcg ggggatcaag cctcgcgcta ttcgggcaat ggtcttaggt
      781 attccaaatg ttgggaaatc tacgttgatg aatcgactag taggcaagaa aattgcacag
      841 acaggaaata agcctggcgt aacaaaaggt cagcagtggc ttcgtttagg taatgagttg
      901 gaattgttag acacaccagg gattttatgg cccaaatttg aagatgaaga aatcggcaaa
      961 aaactagcgc tgacaggcgc gatcaaagac cagcttgtcc atttagatga tttagcgatt
     1021 tacggattag acttttttgc ccgatattat tcaggaagaa tcagcgatcg ttatggttta
     1081 tcaatcgagc aagaagaaaa attggcaccg gaattactga tggatattac agcaaaacgt
     1141 gggtataaag aagactatga tcgcggcagc gaattagtcg tgcatgatat tcgccaagga
     1201 aaacttggac gatacacact ggatcgttgc gaagaaatgg agcaaaaaag tgatgactga
     1261 atccatccaa agtatcaaag aacgattgaa aagcatacag agaatagatg atcctacact
     1321 agaatcatta agaaatgatc cacgcaaagg cgtgcagcaa gcactggctt cttttgaacg
     1381 acgattgaaa aaggaagcat taattaagga aaaatatgaa gaaatgtttt gctttgaaaa
     1441 gcaagggtac gcacaaggtc accaatggat tgccggcatt gatgaagtag gaagaggacc
     1501 attagctgga cctgtcgtgg cagctgcagt tatcttgccg aaaaatgaga tcatattagg
     1561 gctgaatgac tccaaacaat tatcagagaa aaagcgagaa cttctttatc aacaagttca
     1621 agaaaaagcc ctggcaatcg ggatcgggat cgttgatcag cagacaatcg atgatgtgaa
     1681 tatttatgaa gcaagtaaat tagcgatgat ccaagcagta gaaaaattgt ccattgtacc
     1741 aagctatttg ttgatcgatg cgatgacctt ggatttgcct atccagcagg aaaaaataat
     1801 caaaggggat gcacgatctg tgtcgattgc tgctgcaagt atcattgcta aagtatatcg
     1861 agaccaattg atgaaagaat atgacgtgct ttatcctgga tatggtttct cgcataatgc
     1921 aggttatgga acaaaggaac acttagaagg ccttgaacga caaggaatta cgccaatcca
     1981 tcgtaaaagt tttgcacctg taaaaaaata tttacaataa aaaacagccg cttttacgta
     2041 tcttattaga aagagagacg aaaggcggtt ttatgatgta tcaaatagaa gagaatttat
     2101 tgaaacttgc ttatgcaaaa ggaatcagta gtcaaggaaa atgggcactt gctaactgga
     2161 tgatgcagta tccttataaa gaaatcggat ttgaagacat cattaagata ggtaaaataa
     2221 ccacacatcg ggagctgttt cgcaaaagtt ggcaggaaat tcacgctaac tggggcaaaa
     2281 tccaatcgaa acagtcgttt atcacttgct ttgatcccaa atatccccct cagctacttc
     2341 acttgaccta tccgccaatt gtactgtttt ataatggtga tctatcttta ctttcttgta
     2401 acatgttaag cgttgttggt ggaagacaca cttctgcttt agccaaaaaa acagtttatt
     2461 atttactaaa accagtggta gaagctggat atgtgatcgt cagtggctgc gcgaaaggga
     2521 tagatacata cggtcaacaa gcagcaatct ctcatggtgg acggacaatc gcagtcattg
     2581 gaacagggat agagcaagtc tatcctgctg aaaatcgtga cttgcaagag gtgattgcaa
     2641 atgatcatct tcttttgagt gaatatcctc ctcaatccgg cccgaaaaag catcgttttc
     2701 caatgagaaa tcggattatc gcagctttgt ctaaaggaac atgtgtcttt gaagccaaga
     2761 aaaaaagcgg ctctctgata acagcacagc aagcattgga tcttggaaga acagtttttt
     2821 ctgttccagg aaatatttta acagaacatt ctaccggatg tcatcatttg atccaagacg
     2881 gtgcagtttg cacggtttcg ggtgaagata tcttggcaga atttaaagaa taacaccata
     2941 cttttcattt gcttttcttg acaaacgttt tctgaatgga tatgattagc tacgatttgt
     3001 atgaatcctc ttttaatagt aggaacacga caatggaaag gagtccattc agtaatggcg
     3061 tataaatatt tagtgattgt agaatcacct gcaaaagcca agacaattga gaaatatctt
     3121 ggacgaaact ataaagtaat ggccagtgtt ggccatataa gagatttacc gaaaagtaaa
     3181 atgggtatcg attttgaaaa caattatgaa ccccattata tttctatacg cggaaaaggc
     3241 gatgtcatca aaagccttaa agccgcagca aaaaaagctc aaaaagttta cttggcaagt
     3301 gacccggata gagaaggaga agcgattgct tggcatttag cgtacctact tgggttggat
     3361 ctgaaagaaa aaaatcgggt ggtcttcaat gaaatcacaa aagacgcagt caaagcagct
     3421 tttaaggaac caagaacgat cgatgtagat ttagtagatg cacagcaagc tcgtcgtacc
     3481 ttagacagaa tcgttggtta ttcgatcagt cctattctct ggcgtaaggt caagaaaggg
     3541 ttaagtgcag gacgtgtcca atctgtcgct ttaaaaatta ttattgaccg tgaaaaagag
     3601 atccgagaat ttgttccaga agaatattgg agcatcgacg gtaattttaa aaaagctcgc
     3661 aagaaattca aagcaaattt ctggggaatc gacggtaaga aaaagaaatt accagatgca
     3721 caaagtgtaa aagaagtcac tgctagaatt gatggaacag acttcgaagt taaaaaagta
     3781 gaaaagaaag agcgcaaaag aaaccctgcg aatcctttca ctacaagtag cctgcaacaa
     3841 gaagctgccc gtaagttaaa ctttcgaaca agaaaaacca tgatggtagc acagcaatta
     3901 tatgaaggaa tcactttggg taaacaagga acagtcggct tgattacata catgcgtact
     3961 gattctaccc ggattgccga tacagcaaaa gcagaggccg cagcatttat tgaagatacg
     4021 tatggaaaag aattttctag acatgatcac cgaaagatca aaaatgctca aggcgcacaa
     4081 gatgcccatg aagctgtgcg tccaacgagt gtgcttcgta caccagatga actcaaacaa
     4141 tatttagata aagatcaatt gaaactttat acattgatct ggtcacgttt tgtggctagt
     4201 cagatgacac cagctatttt agatacaatg aaagtgacat tagaacaaaa tggtgtcatc
     4261 ttcattgcga acggttcaaa aatcaaattc aaaggattca tgcaagtgta tgtggaaggt
     4321 agagatgacg gcaaagaaga gaaagaaaat attcttccag aacttgaaga aggcgatata
     4381 gtccaatctg ttgatattga accaaaacaa cattttactc aaccaccagc acgttttagt
     4441 gaggctacgt tgattcgtgc actagaagaa aacggggtag ggcgaccttc cacttatgca
     4501 ccaacacttg atacgatcca aagacgctac tatgtaaaat tgacacaaaa aagatttgag
     4561 ccgactgaac taggagaaat tgtcaattca ctgatctgtg aattcttccc tcaaatcgtc
     4621 gatatccatt tcacagcaga aatggaagga gatttggata aaattgaaga aggcacggaa
     4681 gcgtgggtca aagtggttga tcgtttctat aaaccgtttg aaaaagaact gacgaatgcg
     4741 gaagaaaaaa tcgaaaaaat tcaaatcaaa gatgaaccag caggattcga ttgtgatgtc
     4801 tgtggacatc cgatggtaat aaaattaggg aaatatggga agttttatgc ttgcagtaat
     4861 tttcctgatt gtcggaatac gaaaccaatc gtaaaagaaa tcggtgtgac ttgcccagta
     4921 tgtcatgaag gacaagtcat tgaacgaaaa tctaagaaaa acagactatt ctatggttgt
     4981 tcccgtttcc cagaatgtga attcacttct tgggataaac cagtgggcag aaattgtcca
     5041 aaatgtgacg gatatctggt cgagaagaaa gtcaaaggcg gtaaacaagt agtctgtgta
     5101 aatggagatt acgaagaaga catccaaaaa taatagaaaa gaggttcttt cattgaaaat
     5161 ggtaaatgtt gttggagcag ggcttgccgg aagtgaagct gcttggcaaa tcgcacaagc
     5221 aggagtccct gtccgtttat atgaaatgcg tccagtcaaa tcgactgagg cgcatcacac
     5281 aagtaatttt gctgaactag tatgttccaa ttcattaaga ggaaacagtt tggctaatgc
     5341 agtagggtta ttgaaagaag aaatgcgccg attggattcg gtagtaatca cttcggcaga
     5401 tgaaacagca gtgccagctg gtggtgcctt agctgttgac cgcgattctt tttctgaaac
     5461 aattacccga aaagtcaaag agcatccatt agtaacagtg ataaacgaag aaattactga
     5521 aataccggaa ggaatcaccg tagtagcaac aggacctttg acttctcatc cattagcaga
     5581 aagcatcaaa gcattcaatg gttcagatgg tttttatttc tatgatgcag ctgcaccaat
     5641 cgtagacaaa gctacgatcg atatggataa agtatatttg aagtcccgct atgacaaagg
     5701 agaagcagct tacttgaact gtccgatgac agaagaggaa ttcaaagctt tccatgaggc
     5761 actaatcaat gccgaagtgg cagaacttaa atcttttgag aaagaaaaat attttgaagg
     5821 atgtatgcca attgaagtaa tggcacagcg aggattcaaa acgatgcttt tcggacctat
     5881 gaaaccagtt ggattggaag atcctaaaac tggcaaacgt ccttatgctg ttatccagct
     5941 gcgtcaagac aatgctgcag cttctttata caacatcgtg ggctttcaga ctcacttgaa
     6001 atggggcgag caaaaacgtg ttttccggat gattcctggg ttagaaaatg ctgaattcgt
     6061 tcgctatggt gtgatgcacc gaaacagctt tatgaattcg ccagaattgc tgaaaccgac
     6121 ttaccaatca acgaaacgcg aagatctgtt ctttgcaggt cagatgactg gtgtagaagg
     6181 ctatgttgaa agtgcagcga gcggactact tgctggaatc aacgccgcac gcttagcaaa
     6241 agaaatggaa ccagtcgagt ttccacaaga aacagctatt ggaagtatgg catactacat
     6301 tactcatgcg gaaggaaaac atttccaacc gatgaatgcc aactatggtc tgtttcctga
     6361 actgccagag cgcattcgag acaaaaaagc aagatatgaa gcaattgcac aacgagcttt
     6421 aaaagctaat gaggcaataa aaacagaatt agaaaaaact gcaatcccaa atgagtaagg
     6481 aaactccatg aagagggtga gacggatagt ctcgctcttt ttcttgcgtt tttcttaaga
     6541 atttctcaaa caaaatgtga ggaattgcgc caattgtgaa caaacttaga aatattggtt
     6601 aaattctgac aatttatttg tctttttata aacgctgtgc taaattattc tctgggacaa
     6661 aaggaaggtg agaaaatgac agaagccacg tgggaacaaa aatttttgcg ttatttgatt
     6721 gtcgaaagag gatattcaga aaaaacgaaa gaagcatatg aagaagacat tctccatttc
     6781 aaacattttt tggaggaatc tggagacgct gatctgctga aggcggaata ttttacggtt
     6841 cgtgtctatt tgagtgcatt agctgatcat cgtcctagct atagcagaaa ttctatcagt
     6901 cgcaagatag ccagcctacg ttcgttttat cagtatcttc tcaaagaaga agtgataaaa
     6961 gaaaatccat tttcttacgt tcatctcaaa aagaaaaatt tgagactgcc acgttttttt
     7021 tatgaaaatg aaatgcaagc actttttgac agcgtagcag gagatagtcc tttggatttg
     7081 aggaaccgcg ctttactgga agtcctttac gggtcaggca tccgtttaag tgagtgtagc
     7141 agtttgtcgg tatcagacat tgattttgat tcagaggtca tgttgatcca tggtaaaggg
     7201 aacaaagaga gatatgctcc tctaggttct tttgctcaag atgctctgca agaatatttc
     7261 agggaaggcc gccaggtatt gatgacaaaa tatcacgaag agcatgatat cgtttttgtg
     7321 aatcatcatg gaaaaccaat cacgcctact ggaattgaat atgtcttgaa tcaaatgatc
     7381 aaaaaaagca gtttggatag tgatatccat cctcacatgc tgcgccatac atttgctact
     7441 catttattaa ataatggggc agatttgcgg acagtccaag aattgctagg acatgccaat
     7501 ttatcgacta cccagatcta tgcccatgta acaaaagaaa gtttacaaaa aaactatcgt
     7561 tctttccatc caagagcgta aaaataagga aaagaacaa
//
LOCUS       DAKBVG010000126         7509 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000126 DAKBVG010000000
VERSION     DAKBVG010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.126"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(<1..2503)
                     /gene="pglX"
                     /locus_tag="OUD09_001911"
     CDS             complement(<1..2503)
                     /gene="pglX"
                     /locus_tag="OUD09_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HCT9693173.1"
                     /translation="MDKKAIKNFAVNARRKLIDEVMTKAKRLGIDESKIEDVKKIDSN
                     LQEIEKSGIRIQGKEIAQRNKLIAELKQRASVTSYSEAFQELIEEVAYTWFNRLIAIR
                     FMEVNDYLPDTYRVLSSEISGKIEPDIITDIYDADIFEELSSNEQQQVRTWKTDNSAE
                     AMDKLYQLIFIKICNQLNDYLPELFEEVEDYTELLFTASYIKKDGVIADLLSIPEDDF
                     YVQAGGQVEIIGWLYQYYNSEPHNQVVNIYKGIVKKQDIPAATQLFTTDWVVRYMVDN
                     SLGKYWLDHYPNSELRNKLKYLIPDKYAFEQATSVSNLKPEELKIIDNAMGSGHILVY
                     AFDVLMEIYLTLGYSERDAARAIVEYNLYGLEIDKRAYQLSYFAIMMKGRQYDRLFLK
                     KSLSPNLREFIDSPKVTDEYQERISELNNDKYSERWNEYKELIFDQFENGKELGSIIK
                     LVDKNNQAITKEYLGKVREYISKFNEFTNMDILYGMKEIQEKYLHILDVAEMLVDKYV
                     AVVTNPPYLNKMDKKLKKYVNDNYSDVKKDLFSVFIKLNSQMLIDNGYASFMTPFVWM
                     FISSYEKLRSFLINERNISSLIQMEYSAFEEATVPICTFTIKNSNEQDGSYIKLSDFK
                     GGMEVQKIKTLEAIHQHGECDYFYSTNQDNFKKIPGMPIAYWASDNVITDFAQGKLTN
                     DVADAKQGLATGNNNKFLRYWFEVEFNKIKFDAKTLEDAKKSRKKWFPTTKGGRFRKW
                     YGNNEYVVNWENDGDEIKNYVNENGKLKSRPQNLNYYFKKGITWSTLSSGQVSFRLQN
                     QAIFETKGSVLFSKDITDFEEIFGYVNSKLVNHFLT"
     gene            complement(2567..6238)
                     /gene="brxC"
                     /locus_tag="OUD09_001912"
     CDS             complement(2567..6238)
                     /gene="brxC"
                     /locus_tag="OUD09_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HCT9693174.1"
                     /translation="MLIKDIFAKPIDRNIQGVIKVGQAKDENVQQELEEYVVTKELQK
                     HFKTIFDAYQRSINTPTDKMGVWIQGFFGSGKSHFLKIISYLLANREINDKKAIDYFK
                     EDGKIKDPLTLATMERCSQINTDVILFNIDSKSDAGGKKDKNGIVNVFLKVFNEMQGL
                     STIPHLADFERRLIKEEKFDAFKAAFEEINGAKWQEERHEFDWIQDDIKEAALKIDFM
                     SEAAIDNFLEKASGNYLISIDDFANLVKSYLDSKEADHHIAFLVDEVGQYIGGNRELT
                     LQLQTVVEDLGRICEGKAWVLVTSQEAIDHVTNFDNNSDNDFSKIKDRFATTVSLSSA
                     NADEVIKERILKKKEFANDSLALQFETVQTELKNKLLFVDSPELKLFQDAENYAEVYP
                     FIPYQFNLLGQILTEIRNHSYQGSNLSSGERSLLAMFKEAAERNKENDTTSLVSLDQF
                     YYSLERWIDTAISRVITQATNNSHIQRPTDIDNFNVNVLRILFMIKYVDQAIKPNVEN
                     ITSLMIRNINEDRLETKKRVEEALDLLIKENFVRKNLDSYIFQTDEEQEITREIKNQN
                     ITDSDLIVELSKRIFDGVLDRNSFAYEKLGMSGGNKFKNRYTFTYAQVLDQRPYKQAG
                     NAEMTVNFISPYSDLAGQEAELALKSSRNLLFVELPSDKEFKDDIAMYARMNLYLTTT
                     YSSVVKNFDAIRSEKSGELQELSASINDQLNELLKSAKFFYNGAEIQLTGSDFKAKFQ
                     SALQKMAEEVYHKLNQIDAPKEKEDILYLVKESKNEIIEDPSNEQAVQEIFNFINDQK
                     RNHLTITMKTIRDRFETKQPYGFTELDVEWCIAKLFMSGKIDLLYNNVQISRMDDPNS
                     IVMIIQKRRESEKIKIEPSAVISPKAKKLVDEIGRELFQSRNIVDENNNEHTMENFKK
                     YLKGQLDNLRSYFDLQNTYTYPSAYEYEQIKKYLEVIVQTSDVEDFFYTIKKYRDELF
                     DWAETYDKIKNFHKQNSTQKKIWENAQDKIRLIEKSRRQTSSQEIDQLIHEMKTILKK
                     ADPYSDMMKLNELIQEFIEQYNQLLELESQEILNKLTEEKQATFKLLEGKDFKDQFVP
                     IVDQEFQELKAKIEQANTIDDLVFLSLNVAEQYLIFNQRFDNEENRRRQEEQARKQIE
                     AANLIRETEKDEIDSAAKAEEIAIVKPTIQQKTVPMRTLNVHVKTIKSAADIDELTAE
                     INQKLKEQLQENTEITIQF"
     gene            complement(6263..6838)
                     /locus_tag="OUD09_001913"
     CDS             complement(6263..6838)
                     /locus_tag="OUD09_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HCT9693175.1"
                     /translation="MSLSIQEHLEKLNEVIQKEDFLLNRGLGNEVGYYIFDYDPKDEL
                     IVRDYINDISGTSVGDNYEVKVYNLYVIMLEYLEKRGFTEKAIKMEERRGLQKLVESI
                     GRLLKMDLPEEENVFFEYVDAHTPEKSVVFLTGIGELYPLIRAHSILNKMHQVFDRVP
                     VVMMYPGEYDELSLTILNTNKDDNYYRAFRI"
     gene            complement(6838..7437)
                     /locus_tag="OUD09_001914"
     CDS             complement(6838..7437)
                     /locus_tag="OUD09_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HCT9693176.1"
                     /translation="MNKKYSAGLVSKRFWFSEFKLYIQLRLEGYTDIEIKEKNEHENI
                     FFAVSSNRQKEIYQNVKRRVSQLDEAGLQLFNQLNIDNQKLVNLISILMIDDLFNEFM
                     TEVYAENLRKGNLQLTPVDYRSFFTEKRRSSEDISKWKDYTIKRLIAAYQTNLIETGL
                     VRGEKEYGRMTPILLDKRLIQWLVDKNRKDIVLALGGIL"
BASE COUNT         2225 a         1417 c          995 g         2872 t
ORIGIN      
        1 aagttaaaaa gtgattaact aatttagagt ttacatagcc aaatatttct tcaaagtcag
       61 ttatatcctt tgagaacaga acagatcctt tagtttcaaa aattgcctga ttctgcaatc
      121 taaaagatac ttgtccagat gaaagtgtcg accaagtaat tcccttttta aaatagtaat
      181 taagattttg tggtctagat tttaacttcc cattttcatt aacataattt tttatttcat
      241 ctccatcatt ctcccagtta accacgtatt cattgtttcc ataccatttt ctaaaacgac
      301 cacctttagt tgtaggaaac catttttttc tagacttttt agcgtcctct aatgttttag
      361 catcaaactt tattttgtta aattctactt caaaccaata ccgcaaaaat ttattgttat
      421 ttccggttgc caacccttgt tttgcatctg caacatcgtt agttaatttt ccttgagcaa
      481 aatcagtgat tacgttatcg cttgcccagt aagcaatcgg cattcctggg atttttttga
      541 agttgtcttg attagtacta taaaaataat cgcattcacc atgctgatga attgcttcta
      601 acgttttaat tttttgaact tccattccac ctttaaaatc tgataatttt atatagcttc
      661 catcttgttc atttgaattt tttatcgtaa acgtacaaat tggaacggtt gcttcttcaa
      721 aagctgaata ctccatttga atcaaactag aaatgtttct ttcattaatt aaaaatgagc
      781 gtaacttttc atagctagaa atgaacatcc aaacaaatgg cgtcataaag gaagcatatc
      841 cattatcaat cagcatttgg ctattcaatt taataaacac agaaaataga tcttttttga
      901 catcagaata gttatcattt acatattttt ttaatttctt atccatttta ttaagataag
      961 gcggattagt gactaccgct acatatttat caactagcat ttcagccacg tcaagaatgt
     1021 gcaagtattt ttcttgtatt tccttcatcc cataaagaat atccatattc gtaaattcgt
     1081 tgaattttga tatatactct cttacttttc ctaagtattc tttagttatt gcctgattat
     1141 ttttatcgac taacttaatg attgatccaa gttctttccc attttcaaat tgatcaaaaa
     1201 ttaactcttt gtattcattc cagcgttcac tatacttatc attgttcaat tcactaatac
     1261 gctcttgata ctcgtctgtc actttaggag aatcaataaa ctctcttaaa ttcggcgata
     1321 gacttttttt caaaaaaaga cgatcgtatt gacgcccttt catcataatt gcaaaataag
     1381 acaattgata cgctctttta tcgatttcaa gaccatataa attgtattca acgattgcac
     1441 gtgcagcatc acgctcagaa tatcctaacg taagataaat ttccatcaat acatcgaagg
     1501 cgtaaactaa aatatgccca gaccccattg cgttatcgat aatttttagt tcttctggtt
     1561 ttaaattact tacagatgtt gcttgttcaa aagcatattt atctggaatt aagtatttca
     1621 acttatttct taattcacta ttgggataat gatctaacca atactttcct aaactattgt
     1681 caaccatata acgaacgacc caatctgtgg taaatagttg agttgctgcc ggaatatctt
     1741 gttttttgac aatcccttta taaatattca ccacttgatt atgtggctca ctattatagt
     1801 actgatacaa ccacccaata atctctacct gtccaccagc ttgaacatag aaatcatcct
     1861 ctggaatact taaaagatca gcgattacac catctttttt aatataagaa gctgtaaaca
     1921 aaagttctgt ataatcttct acttcttcaa atagctcagg taagtaatca ttcaattgat
     1981 tacagatttt tataaaaatc aattgataca gcttgtccat tgcttctgct gaattatctg
     2041 ttttccaagt tctgacttgt tgctgttcat tactagataa ttcttcaaag atatcggcat
     2101 cataaatatc tgtaataata tctggctcaa ttttcccact tatttcacta gataatacac
     2161 gatacgtgtc aggtaaataa tcatttacct ccataaaccg aatggcaatc aatcggttaa
     2221 accacgtata agcaacctct tcgattaact cttgaaaagc ttctgaataa gaagtgacag
     2281 aagcacgttg ttttagttct gcaatcaatt tgtttctttg cgctatttct tttccttgaa
     2341 ttcttattcc tgatttttcg atttcttgta aattgctatc aattttcttc acatcttcaa
     2401 tttttgattc atcaattcct aaacgttttg ctttagtcat tacttcatca attaattttc
     2461 tacgggcatt gaccgcgaaa ttctttatcg ctttcttatc catttttctt tcccttctat
     2521 atgtaacctt tgaaaagaca ggcacctttt tatgcctgtc ttttcactag aattgaatcg
     2581 tgatttctgt attttcttga agttgttcct tcagtttttg attaatttca gctgttaact
     2641 catcaatatc tgctgctgac ttaatagttt taacatgaac gttaagtgtt ctcattggta
     2701 ctgttttctg ttgaatagta ggttttacta tcgctatttc ttcagctttt gctgcagagt
     2761 ctatttcatc tttttcagtt tcacgaatca agtttgctgc ttcaatttgt ttacgtgctt
     2821 gttcttcttg acgtctgcgg ttttcttcat tatcgaaacg ttgattaaag atcaaatact
     2881 gttctgctac gtttaatgat aagaaaacaa gatcatctat agtattcgct tgttcaattt
     2941 ttgctttcag ttcttgaaat tcttggtcaa ctattggtac aaattgatcc ttaaaatctt
     3001 ttccttctaa taatttaaat gttgcttgtt tttcttccgt taatttattt aatatttcct
     3061 gactttccaa ctctaacagt tgattatatt gttcaataaa ttcttgaatc aactcattta
     3121 gtttcatcat atctgaataa ggatcagcct ttttcaaaat ggttttcatc tcatgaatca
     3181 attgatcaat ttcttggcta gaagtttgac gccgactttt ttcgattaat ctaattttat
     3241 cttgtgcatt ctcccagatt tttttctgag tactattttg cttgtggaaa tttttaattt
     3301 tatcatatgt ttcagcccag tcgaataatt catcgcgata ttttttgatt gtataaaaaa
     3361 agtcttcaac atctgacgtt tgaacaatta cttcaagata tttttttatt tgctcatatt
     3421 cataagcact agggtaggta taagtgtttt gtaaatcaaa ataagagcgt aaattgtcta
     3481 attgaccttt taaatatttc ttgaaatttt ccattgtatg ttcattgtta ttttcatcaa
     3541 caatattacg actttgaaac agttctctac cgatttcgtc aaccaatttt tttgcttttg
     3601 gcgaaatcac cgcgcttggt tcaattttta tcttttcact ttctcgtcgt ttttgaataa
     3661 tcattacaat actatttgga tcatccatcc gtgaaatttg tacattgtta taaagtaaat
     3721 caattttacc agacatgaaa agtttagcga tgcaccattc cacatcaagt tctgtgaatc
     3781 catacggttg ttttgtttca aaacgatcac gaattgtttt catcgtaatc gttaggtgat
     3841 ttctcttttg atcattgata aaattgaaaa tttcctgaac tgcttgctca tttgatggat
     3901 cttcaataat ttcatttttg ctttctttca ctaaatataa gatatcttct ttttctttag
     3961 gcgcatcaat ttggtttaat ttatggtata cttcttcagc cattttctga agtgctgatt
     4021 ggaattttgc tttaaagtcg gagcctgtca gttgaatttc tgcaccgtta tagaaaaatt
     4081 ttgcagattt caacagttca tttaattgat cgttgatact tgcacttagt tcctgtaatt
     4141 caccagattt ttccgaacga atcgcgtcaa agttctttac tacactcgaa taagtcgttg
     4201 ttaaataaag attcattcta gcatacattg ctatatcatc tttaaattct ttatctgatg
     4261 gcaattctac gaataataag ttacgggaac tcttaagcgc aagttctgcc tcttgacctg
     4321 ctaaatcaga gtatggtgaa ataaaattaa ctgtcatttc agcatttcca gcttgcttgt
     4381 atggacgttg atctaacact tgtgcataag taaacgtata ccggttttta aatttatttc
     4441 cacctgacat gcctaatttt tcataggcaa aactatttcg gtctagaacg ccgtcaaaaa
     4501 ttcgtttaga tagttctacg atcaaatcac tatctgtaat attttgattt ttaatctcac
     4561 gagtaatttc ctgttcttca tcagtttgga aaatataact atctaagttc ttccgcacaa
     4621 agttttcctt aattaaaagg tccaatgctt cttctactcg ttttttagtt tccaggcggt
     4681 cttcgttgat atttcttatc atcaagctcg taatattttc aacatttggt ttgatggctt
     4741 gatcaacata tttaatcatg aataaaattc gtaagacatt tacgttaaag ttatctatat
     4801 cagttggtct ttgaatatga gaattatttg tagcttgtgt aatcactcgg ctaattgctg
     4861 tatcaatcca acgttctaac gagtaataaa actgatctaa agaaaccaaa ctggttgtat
     4921 cattttcctt atttcgttca gcggcttctt taaacattgc tagtaatgaa cgttcaccag
     4981 acgataagtt tgatccttga tagctgtgat tacgaatctc ggttagaatt tgccccaata
     5041 aattgaattg atagggaata aatggataaa cttctgcgta attttcagca tcttggaaga
     5101 gctttaattc aggactatca acaaagagta atttattttt caattctgtt tgaacagttt
     5161 caaattgtaa ggctaaagaa tcattggcaa actctttttt cttcaaaata cgttctttaa
     5221 ttacttcatc ggcatttgct gaagataaac tcacagttgt ggcaaaacga tccttgatct
     5281 tagaaaaatc attatcagaa ttgttatcaa agtttgttac gtgatcaatc gcttcctgac
     5341 ttgtcactaa tacccaagcc ttaccttcac aaattcgacc caaatcttcc acaacggttt
     5401 gtaactgcaa agtcaactca cgattaccgc cgatatattg tccgacctca tcgactagaa
     5461 aggcaatatg atggtctgct tctttggaat caagatagct tttaactaga tttgcaaagt
     5521 catcaatgct tattaaataa tttcctgatg ctttttctaa aaaattatca atagcggctt
     5581 cactcataaa atcgatttta agtgccgctt ctttgatatc atcttggatc caatcaaact
     5641 catggcgctc ttcttgccat tttgccccat taatctcttc aaaagcagct ttgaatgcat
     5701 caaacttttc ttctttaatt aaccgacgtt caaaatctgc tagatgtgga atcgtgctta
     5761 atccttgcat ttcgttaaat acctttaaaa aaacattcac gattccgttt ttgtcttttt
     5821 taccacctgc atcacttttt gaatcaatat taaacagaat aacatctgta tttatttgtg
     5881 agcaccgttc catcgttgcc aaagtcagtg gatctttgat tttcccatct tctttgaagt
     5941 aatcaatggc tttcttgtca tttatttctc gatttgcgag caagtaagaa ataattttta
     6001 agaaatgaga tttacctgaa ccaaagaacc cctgaatcca aacacccatt ttatctgttg
     6061 gtgtattgat actccgttgg taggcatcaa aaattgtttt aaagtgtttt tgtagttctt
     6121 tcgtaaccac gtattcttct aattcttgtt gaacattttc atcctttgct tgaccgacct
     6181 taataactcc ttgaatattt cggtcaattg gttttgcaaa aatatcttta attagcattt
     6241 aatctcctac cttccatccc aactaaattc taaatgctcg ataataatta tcatctttat
     6301 tggtattaag aattgtcaaa cttaattcat catattcccc tggatacatc atcacaactg
     6361 gtacgcgatc aaatacctga tgcattttat ttaaaataga gtgcgctcta attaatggat
     6421 ataattcgcc aattcctgtc aggaacacaa ctgatttttc tggtgtatgg gcatccacat
     6481 attcaaaaaa tacattttct tcctcaggta aatccatttt caataaacga ccaatgcttt
     6541 ccactaattt ttgtaaacca cgtcgttctt ccatcttaat cgctttttca gtgaaaccgc
     6601 gtttttctag atactctaac ataatcacat agagattata aactttcact tcataattat
     6661 caccaacaga tgttccagat atatcattaa tataatctct tacaattaac tcgtctttcg
     6721 gatcgtagtc aaagatataa tagcctactt cattacctaa accacgattc aataaaaaat
     6781 cttctttttg aattacttca ttcaactttt ccaaatgctc ttgaatcgat aatgacatta
     6841 taaaattcct cccagtgcta gtacaatatc tttacgattc ttgtcaacta accattgaat
     6901 caatcgttta tctaataata tcggagtcat tcgtccatat tctttttccc ctctgactaa
     6961 tcctgtttca attagattcg tttgataagc ggcaataagc cgtttaatcg tataatcttt
     7021 ccattttgaa atatcttctg aacttctccg cttctctgta aaaaaagaac ggtaatcaac
     7081 tggtgttaat tgcaaatttc ctttacgtag attttcagca tatacctcag tcataaattc
     7141 attaaaaaga tcatcaatca taaggatgga aatcaaattc actaactttt gattatctat
     7201 atttaactga ttgaaaagtt gcaatccagc ttcatccagt tgactaactc tccttttcac
     7261 gttctgatag atttcttttt gacggttact gctcactgca aaaaaaatat tttcatgttc
     7321 atttttctct ttaatttcta tatctgtata tccttctagt cgtaattgaa tatataactt
     7381 gaactctgaa aaccaaaagc gtttagaaac tagaccagca gaatattttt tattcaatag
     7441 attgcctcct aatcatctat aattcaataa gtaaacaagt gttttttgct catcgttagc
     7501 attcatttt
//
LOCUS       DAKBVG010000127         7507 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000127 DAKBVG010000000
VERSION     DAKBVG010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.127"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            <1..618
                     /locus_tag="OUD09_001915"
     CDS             <1..618
                     /locus_tag="OUD09_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693177.1"
                     /translation="LKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKEEKVPEYA
                     LLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQFSQTLARF
                     IPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKRSPFNDNK
                     TRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            690..1085
                     /locus_tag="OUD09_001916"
     CDS             690..1085
                     /locus_tag="OUD09_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCT9693178.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            1069..3522
                     /locus_tag="OUD09_001917"
     CDS             1069..3522
                     /locus_tag="OUD09_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCT9693179.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            3519..5546
                     /locus_tag="OUD09_001918"
     CDS             3519..5546
                     /locus_tag="OUD09_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693180.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            5543..6565
                     /locus_tag="OUD09_001919"
     CDS             5543..6565
                     /locus_tag="OUD09_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HCT9693181.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            6582..>7507
                     /locus_tag="OUD09_001920"
     CDS             6582..>7507
                     /locus_tag="OUD09_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCT9693182.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIV"
BASE COUNT         2519 a         1313 c         1504 g         2171 t
ORIGIN      
        1 cttaaactat taaaactaga catgaaagat aaaggctggg tgatagattc cttttacttt
       61 cgttacaaac agcaaaatta tattgtactt gttaagctat ttgaaaaaga ggaaaaagtt
      121 ccagaatatg ctctgttaaa actagaattt ttaaaggaaa acgatttttc agatatgcta
      181 gctgtctatg ctaattcggt aaaattatat acagacacga aaacaataag agaatatttt
      241 ggtatcgaat acagtagtaa cttgggagat gttttatttc aatttagcca aactcttgct
      301 cgatttattc cgacagaagt tagtgagaag aaaaatgaag accaaaaaga agccatgtgt
      361 ttctcattaa gtcaaagtga ttcagaagat cccagaaaaa aatattgttt ttcagttaga
      421 agaaatcctt taaagggaaa tggtgagctg ggaaaacgaa gtccttttaa tgacaataaa
      481 acgagattgt atagacccag cttatatgaa cgattagggt cagataccaa tttaagtttc
      541 agatattcaa tgaatccaga tgatgaagaa actgatgaag gtattattgc aaaatggaca
      601 aaaaataaaa ttgaatagtt aaagcattga aagagcaatc acctattttg attgttcttt
      661 ttctattttt acggaaagga cgtgaagaca tgaaaaaaat aagaagttac accagtattt
      721 ggtcagtaga aaaagtgatt tatgcgatta atgatttaca actccctttc ccaattacct
      781 ttacgcaaat gacatggttt atcgtttctt tgtttgtcgt catactcttt ggcagtttac
      841 caccactttc tctgattgac ggagcactac tcaaatactt agggattccc attgttctca
      901 ctgggttcat gtgccaaaag acctttgacg gaaagaaacc ctatggattt ctaaagtcta
      961 tcattaccta tgctctacgc cctaaagtaa cctacgctgg caaacaaata cgattaaaaa
     1021 aagaaacaat gaatgaatcg attacgattg taaggagtga acaactagat ggcatatccg
     1081 attaaatata tggaagataa tctcgtattt aatcatgatg gcgaagtatt cgcttattat
     1141 gaattaattc cctataatta cagtttttta agttccgatg aaaaaattca agttcatgat
     1201 aatttctgtc aattgattgc ccaaaatcgt gatggcaaga ttcatgctct gcaactagca
     1261 accgaatcaa gtattcgagc cacacaggaa caatcgaaaa aagaaatcac tggacgatta
     1321 aaagagatag cttacgaaaa aatagatgaa cagaccgaag cgttaatttc tatgattggt
     1381 gacaatcaag tagactatcg cttttttatt ggttttaaac tctacctcaa tgaacaggaa
     1441 gtatccgtaa aaaatgttgg gaaagaaatt caaaaagcat ttgcggaatt ctttcatggt
     1501 gtgaatcaca aattaatggg cgattttgtt tctatgccta tggcggaaat cgaacgcttt
     1561 tctaagatgg aacagttgtt agaaagtaaa ttatccagac gctttcagat tcgtaaattg
     1621 gagaaagatg attttggtta tattattgaa catctttacg ggcaaacagg tgtccctttt
     1681 gatgactacg actaccattt accgaaacga tacgaagata aaaaagtgtt ggtaaagaaa
     1741 tacgatattc tcaaacccac tcgctgttta atcgaagaaa atcaacgcta tttacggatt
     1801 gaacaggaag ataccactac ttatgtggcg tattttacaa ttaatagtat tgttggtgaa
     1861 ttggatttcc ctaatagtga aattttttat taccagcaac agcagtttac tttccccgtt
     1921 gataccagta tgaatgttga aattgtcgcc aataaaaaag ccttatctac cgttcgcaat
     1981 aaaaagaaag aactgaaaga tttagatagc cacgcatggg aaagcgacaa cgaaaccagt
     2041 actaatgtgg tggacgcttt agaatctgtc gatgaattag aaagtaattt agaccaaacc
     2101 aaagaatcta tgtacaaact aagctatgtg gtacgggtat cagcaaagaa tttggacgag
     2161 ctgaaacaac gttgtgatga agtaaaagat ttttatgatg atctgtctat caaattagta
     2221 cgaccttttg gcgatatgct aggcttgcat aatgaattta ttccctcaag caagcgatat
     2281 atcaatgact atattcaata tgtcacgtcg gattttttgg ctggtcttgg tttcggtgct
     2341 acacaaatga ttggtgaaaa ttctggtatt tacgtcggtt acaatctcga tacaggcaga
     2401 aatatttatc tccaacctag ccttgcaagt caaggaatta aaggctctgt aacaaatgca
     2461 ttagcgtccg cttttattgg ttcattaggt ggcggaaaat cattctctaa caatatgctg
     2521 gtctattatt cagtactctt tggcggacag gcaaccattg ttgacccaaa agctgaacgt
     2581 ggtgaatgga aagaaacctt acctgatatt gcccatgaaa tcaatattgt taatctcacg
     2641 agtgagaacg acaataaagg gttacttgac ccttatgtga ttttaaaact cccgaaagat
     2701 tcagaaagcc ttgccattga tattctcacg ttcttaacag gtatttccag ccgagatagc
     2761 gagaagtttc ctgtcctgcg taaagctatt cggagtgtaa ctcaaagcaa acaacgaggg
     2821 ttgttattgg tgattgatga attgcgaaaa gaagatacgc ccatcagcaa tagtattgct
     2881 gaccatatcg aaagctttgt cgattatgat tttgcccatc tgttattcag tgatggcagt
     2941 gtcacccaat ccattagctt ggaaaaacaa ctcaatatta ttcaagtggc agatttggtc
     3001 ttacccgatt ccaatagctc ttttgaagaa tacactacca tggaattatt aagtgtggca
     3061 atgctgattg tcattagtac ctttgcttta gactttatcc actctgaccg ttccattttt
     3121 aaaattgttg accttgatga ggcttggagt tttttacaag tggcacaagg aaaagctcta
     3181 tctatgcgat tggttcgagc aggacgtgcc atgaatgcag gggtctattt cgttactcaa
     3241 aacgcggacg atttactaga tgaaaagatg aagaataata ttggcttgaa atttgccttt
     3301 cgttccactg acctagtgga aatcaaaaag accttagaat tttttggtgt ggacaaggaa
     3361 gatgaaaaca atcagaaacg cttacgtgaa ttagaaaatg gacaatgttt agtttcagac
     3421 atctatggtc gtgtaggggt tatgcagttt cacccgattt ttgaagagtt gcttcatgcc
     3481 tttgatacca gaccgcctgt gcgaaatgag gtggaagaat gacacaaaaa caaaagaaat
     3541 ttcttcacta tagttggata acgctccttt gtgtgggcgt tattttactt ttattaggca
     3601 cgctgggcaa tgcagtacaa gcgacaggct tagttgatga aaccattgat acaagcaatg
     3661 aatactccaa gtatggtttg aatcaatatc agcttgatta ttatgtggac aatagctggg
     3721 gctggcttcc atggaattgg agtgatggca taggacaatc ggtgatgtat ggattgtatg
     3781 ccattactaa ttttatttgg acaatttctc tttatctatc gaacgccaca ggttatttgg
     3841 tgaaagaagc ctatgcctta gatttcatta gtcaaacatc ccaagcaatt ggcgaaaaca
     3901 tgcagacgat tgcaggcatt acaccgaatg gcttttccag ttcagggttt tatgtcggtt
     3961 tcctccttct ttttattctc attgttggaa tctatgttgc ctatacagga ttggttaagc
     4021 gagaaacgac gaaagtgata cgagctgttt taaactttgt cgtggtgttt attctatcgg
     4081 cttcctttat tgcgtatgca cccgattata ttgcgaaaat caatgatttt tccaaagatg
     4141 tcagtaccgc cagtctgaat attgggacaa aaattgttct tcctaacacg gatagtcaag
     4201 ggaaagacag tgtggacatg atacgtgaca gcttattctc tgttcaagta aagcaaccat
     4261 ggctattgct tcaatatggc tccactgatg tagaagcgtt aggggaagaa cgtgtggaaa
     4321 gtttattgtc taccagtcca gatacaaaca atggtgaaga tagagaaaat gtagtgattg
     4381 aagaaattga agatcatgac aatacctatt taaccctacc gaagacgatt tccagattag
     4441 gaacagtctt ctttttgttt gtattcaata ttggtatctc gatttttgtt ttccttctaa
     4501 caggaatcat gattttttcg caagtgctat ttattatttt cgctatgttt ttgccaatca
     4561 gtttcctatt aagtatgttg ccgagttttg atggcatggg caggcatgcc attaccaagt
     4621 tatttaatgt gattatgaca agggctggta ttaccttgat tattaccgtt gcctttagta
     4681 tttccactat gctttattcg ctgtcagcaa gttctccctt tttcatgatt atgtttttac
     4741 aaattgtgac ctttgcaggg atttatttta aactgggcga tttaatgagc ctattcactt
     4801 tacaaagcaa cgactctcaa aacatgggca gacaagtgtt tcgcagacct cgtcaaatga
     4861 tgaatcgaca aaccagaaga tttcaacgca gtatgagtaa gatttttcgt ggaaaagcag
     4921 ggtcaacgaa gcaaaagaca atgacaacaa agcagaatca aaccaataaa gctaaccata
     4981 ctcgtcaaaa tgaacggact acaccaaaga aagaaacagc actttccaat gtgaaaaagc
     5041 aacaaggcgg tatcgggcaa cgattaggtg aaaagacagc ccatgtattg gacacgaaaa
     5101 atcgtctagt caataaaaga aagcaggcaa aacaacagat acaggcgaca ccaacccatg
     5161 tcaaatatgc ccttcatcaa ggaaaagaga atgtcactca taatgtaaca gactttaaac
     5221 aatctatggc agataccaaa gctcaacgca aacaacagcg tatgaaccag caggagcaaa
     5281 aacgaaagaa cattgctcaa aagcgtttgg agctggataa agcaaaagaa aaaagacgaa
     5341 aagaaaaccc atcatctcct gttcagaata accagcagca tgttcgcaag cgtcagcaaa
     5401 ccgtcacgcc tgttttgtca caaaacacta aacgtgaaaa tcatcagcga ccattgaaaa
     5461 aagagcaaaa acaaaagaca ccaccgaagc aattcaatca gaggaagcta aataaaagaa
     5521 cggataagcg aggacaacaa acatgaagct aaaaataaca gtgatttgta gtgtggtctt
     5581 ctctctcttc ttttttctct tattattcct agtcattttc tttgcagatg atacggacag
     5641 tggcgaaaac aacaaggatt cttctattcc acaaggcggt gtgaccgttt caccagaagt
     5701 gttggcacac cgtccgttga ttgaaaaata tggtaaggaa tatgggattg aagactatgt
     5761 ttcttatatc ttagccatta tgcaagtgga atcgggcggt acagcagaag atgttatgca
     5821 aagttccgaa tctttgggtt taccgcccaa cagtttgagt accgaagagt ccatcaaaca
     5881 aggggttaaa tatttcagtg aattattaac cagtgcagaa caacaaggag tggatataga
     5941 ttctgttatc caatcttata attatggcgg tggcttttta aattatgtga ggagtcatgg
     6001 gaaaaaatat acctatgaat tagccgaaca gttttctaaa gagaagtcgg gtggtcaaaa
     6061 agcagactat cccaatccta tagccatacc tgtgaatggt ggttggcgat ataactatgg
     6121 aaatcagttt tatgttcagt tggtttcaca atacctaacc gacacttccc caacagaatt
     6181 tgatgatgaa accgtgcaag tcattatgga cgaagccttg aaatacgaag ggtttcccta
     6241 tgtctttgga ggggcgtcgc ctaccacctc ttttgattgt agtggcttaa tacaatgggt
     6301 gtatgacaaa gctggcattt ctctccctcg tgtcgcccaa gatcagtatg acgctacgca
     6361 agaaatctct atggaagaag cccaagcagg cgaccttatc ttctttcatt caacctataa
     6421 tgcaggcaca tatgtgactc atgtggcgat atatttagag ggaaatcgct tttatcatgc
     6481 aggcgaccca atcggttatg gcgacttaag cagtcgttat tggcaagacc atctgattgg
     6541 ggctagacgt gttattcata actaagaaag gaatgaatca aatgattata aaaatcgagc
     6601 gaaaagaaaa gaaggagaaa ccaaagaaat ctaaaaaacc gagcaagcaa agaaaagttc
     6661 cgatgattaa agttggcacg cataagaaat taacctttgt tttatgggtt ttattgattg
     6721 gtagtgtcgg atttggaatc tataaaaatt ttactgccat tgacactcat actgtccgtg
     6781 aaacagaaat cattaaacag cagattgtcg ataccaacca agtagaaagt tttgtgaaat
     6841 cctttgcgaa agactacttt tcgtggcaac aatcccaaga agccattgat aaacgaaatg
     6901 aaaaattgac ccattatttg acggaagaat tgcaggtatt aaatgaagaa atgattcgta
     6961 aggatattcc tactagttct agtgtgaacg atatacaggt gtggcaggtt tctcaagtga
     7021 atgagaacac ctttgaggta ctgttttctg ttgaacaagt tattaccgaa gacaaagaca
     7081 aggaaactat atcgtctagc tttcatgtgg ttgtccatat agatgaatca gataatatgg
     7141 ttattatcaa aaatccgaca atgagtaaga aacctcaaaa atcggactac caacccaaac
     7201 agctagaaag tgaccataca gtagatacag aaacgatgga cgaaatcact agctttttag
     7261 aaacgttctt ccagctctat cctaccgcca cagaaaaaga attgacttac tatgtaagta
     7321 atcatgtgtt acctatgatt aacaaggaat atgttttcga ggaattggtg aatcctatct
     7381 ttaccagaaa agataaccaa gtaatcgtga atgtcgctgt gaaatattta gatcaagaaa
     7441 caaaggcaac ccaaatctcg caatttgagt tgatattaga aaaacaagat aattggaaga
     7501 ttgtaaa
//
LOCUS       DAKBVG010000128         7479 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000128 DAKBVG010000000
VERSION     DAKBVG010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.128"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            136..1959
                     /locus_tag="OUD09_001921"
     CDS             136..1959
                     /locus_tag="OUD09_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HCT9693183.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(2101..2286)
                     /locus_tag="OUD09_001922"
     CDS             complement(2101..2286)
                     /locus_tag="OUD09_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCT9693184.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            2762..3025
                     /locus_tag="OUD09_001923"
     CDS             2762..3025
                     /locus_tag="OUD09_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCT9693185.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            3025..3291
                     /locus_tag="OUD09_001924"
     CDS             3025..3291
                     /locus_tag="OUD09_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HCT9693186.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            3492..3980
                     /locus_tag="OUD09_001925"
     CDS             3492..3980
                     /locus_tag="OUD09_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HCT9693187.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(4521..5192)
                     /locus_tag="OUD09_001926"
     CDS             complement(4521..5192)
                     /locus_tag="OUD09_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT9693188.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(5189..6106)
                     /locus_tag="OUD09_001927"
     CDS             complement(5189..6106)
                     /locus_tag="OUD09_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HCT9693189.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            complement(6103..6744)
                     /locus_tag="OUD09_001928"
     CDS             complement(6103..6744)
                     /locus_tag="OUD09_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT9693190.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            complement(6748..7329)
                     /locus_tag="OUD09_001929"
                     /pseudo
     CDS             complement(6748..7329)
                     /locus_tag="OUD09_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
BASE COUNT         2218 a         1439 c         1581 g         2241 t
ORIGIN      
        1 ttttctgttt ttttccaatt attttcttaa agtttattga cttttaaggt cagtgagcgt
       61 actataacat cttgtttact ataaattttc gactataaaa cttgaagtaa aattgtgtgt
      121 aaaggagttt agaaattgga ttacttaaaa aggttgttag taggtaagcc tttaaaatct
      181 gctgaaaatg atgagcacaa attaacccga tttgctgctt tggcattatt gtcgtctgat
      241 gcgttatcat ctattgcata tggtacggaa caaatcgttg tcgttttagt cgctttgtct
      301 gctgcagcca tctggtattc gctacccatt gccgcttttg ttattatttt attgatttca
      361 ttaacgctat catatcgtca aatcatccat gcttatcctc atggcggggg agcatatgtc
      421 gtcagcagtg agaatcttgg aaagaatgct ggcttgattt ccggtggctc tttattgatc
      481 gactacatgt tgacagtagc agtatctgtc tcagcaggtg ctgaagcgat cacatcagcg
      541 gtgccagcac tctacggtca tcaagtcgct atttctgtga caatcgtttt gctactgatg
      601 atgctgaatc ttcgtgggct gagggaatct gcatcgttcc tattatttcc ggtgtacaca
      661 tttatccttg tgatctcgtt actgatcgtt gttggtttat ataatatcgt gacaggtgct
      721 gtgccattgc aagctactgc acttccagga gcggctgtgc cgggtgtttc tatcgctttg
      781 attttacgag cattttcttc tggttcttcc tctttaactg gggtggaggc gattagtaat
      841 gctgtaccat tctttaagaa gccaagagca aaaaatgccg ctgctacatt gacgatgatg
      901 gcattgatct taggtttctt cttcgtgggg atcacattta tcaactattg gtatgggatc
      961 gttcctgaaa aagaagtcac tgttttgtct caaattggta aagctgtctt tggacatggt
     1021 attttgtatt acgtccttca atttgctaca gcgctgattt tagcagttgc tgctaacacc
     1081 ggtttttctg cttttccagt tcttgcatac aatctggcaa aagataaatt catgcctcac
     1141 atgtatcaag atcgtgggga tcgactaggc tattccaatg ggattatcac actagcgcta
     1201 ggttcgatcg tattgctgtt tattttccat ggctcaacag aacgcttgat tccgttatac
     1261 tctatcggcg tgttcattcc atttgcttta tctcaaacag ggatggtcgt caaatggaaa
     1321 aaagaaggaa aaagatggtt aagcaaatcg attgccaata ttacgggtgc gttcatctca
     1381 tatgcaatca ttgcgatttt gtttgtctat cggttagggg acatttggcc tttcttcatc
     1441 attatgccaa tcgttatgtt cattttttat aagatccatg atcattataa aaaagtagca
     1501 gaacagcttc gtttagaaaa tgatgcaaaa ctacatgatt atgacgggaa taccgttctt
     1561 gtattagtcg ggaatgtgac acgggtaaat atcggtgcgt tgaattacgc acgttcgatc
     1621 ggtgattatg tcgttgcgat gcatgtttcg ctagacgaag atgtcgaaaa agaaaaagaa
     1681 atcgaagcag aatttaaaaa gcatttccca gatgtccgtt tttcaatcgt ccactcttct
     1741 tatcgctcga ttgaaaatcc aattatccgt tatgtagata tcgtaagtaa aaatgcagcg
     1801 aaacaaaact atacaactac tgtgttgatt cctcaatttg taccaaacag acgctggcag
     1861 aatattctcc ataaccaaac aagtttgcgg ttaagattgc gattgtcttg gcgtgaaaat
     1921 atcgttgtca gcacctatag ctaccatttg aagaaataaa ccaagtttta aaatcaaaaa
     1981 cagaccctct agctcttaat tgaagctaga gggtctgttt tgtcttattc tcagctaaag
     2041 gtgccctcta taagccatta aagatgggaa cagcgtatga aatgttatta ttcttcctat
     2101 ttagctgtaa aacattttga ttttgatgtc ttgatcataa tttccgaatc ctttgccaaa
     2161 taatgtacag cctcctatat tttcagcttc ttcctttacc tcgactctta agtgccatgt
     2221 gtcttcataa gtaggaatct cagcctcgtc aagtgaccat tttttgttac cataactcag
     2281 tgccattttt aattggtgat tcacaaaccc aacggtctaa gcaagtatca aatcgatagt
     2341 gaacatgaat gacaggagac gaaagaggca atcatttttg aggcagtaga agacttgacg
     2401 gccaaaatca gttttgaata accgttacaa atctttcgtg cgtatcctca ggttattcaa
     2461 gatatcaatc aagtcacact gcaacgagga accttcgtgt attggtgttg ttgcatagac
     2521 tcacaggctt tggattcgac ctttgctaaa gatgatacgt tgttaccaat tactttacgt
     2581 ttggctaccg ctagtcgtaa aggagtaaga agtgagcgtg gacaaagatc ctccctaaaa
     2641 aggggtcttt ttctctatgt aaaaaagagg aaaagtttgc tgaatttaag tgctttattg
     2701 tatttttttg acgtacttaa tcaagtacgc tataattata tcagaaaaag gaggagagac
     2761 aatggctaat acaacgatga atcctagcac tgctcgtaaa aatttttatc agttattgaa
     2821 agaggtaaat gaaaatcata ctgaaatcga gattattagt gatcgtagtg gaaacaatgc
     2881 tgtattgatt gggctggaag actggagagc gattcaagaa accctttttc ttgagcaaac
     2941 aggtacttta gataaagtac gagaccgcga aaaagacgac agtagcttta cgaatatcga
     3001 tgatattgat tgggaagctc tttgatgagt aactatacag tcgcaatcaa aaactctgcg
     3061 aaagtggatt taagaaaaat caagcaaacg aatttaaaga aacaatttga agaagtgatt
     3121 cagacattga aggaagatcc atatatgccc acgcagtctt ttgaaaagct gagacctaca
     3181 catgaaggca gatactcacg caggctgaat agacagcatc gagtggttta caaagttgat
     3241 gaggaaaata aagttgtaga aatttattct gcctggacac attatgagtg aagtgagaag
     3301 agccaacctt tttagatagt taacaaagag tctccccaaa aaggagactc tttttttgtt
     3361 ctaaaaccaa ccttcttgga tttattgcat gatttcttta atttgttcct atatctccac
     3421 ctttgaaggg tcattgtttg agtttaggaa tggaatgttg acgggtttat gtaaaacaag
     3481 gaggaagaag tgtgacaagg aaaatcagta aaattggagc atcgttgtta gtgttggcag
     3541 tggcaacgac catcgattat cgccaaagtg aggcgaagac aattggaaat gtcgtttatc
     3601 gagaaacagc caaaagtatg ttgaaaaaag caatggacaa tcaaccggag tcgtcttatt
     3661 ggttcccaga ggattcgttg gcatggtctt ttggaaaaga tacagatacg aaatacaata
     3721 ccagtgtcgt tcctttagca gagcgggtat ccaaagcgac attgccgaac atgaatgtca
     3781 ctcaaacgga agaagttaaa gtcgtggcgc tttcggccat gaatagcagt atcaacggca
     3841 atgcatcacg agggatcgat atcttttacg ccaatgtctt ttcttattgg caatatatcg
     3901 atcaattggt ttacgggggt gctcttctgg tgaaggatca tcgtatcgcc aagtctagtt
     3961 gcgacggatg cagtccataa gaatcgtgta ccggttttag gaaccgcaaa cctcccatgg
     4021 cgagaagctg gaatggctag atatgttttt agccaaggat gaggatggca agttcccgat
     4081 tgtataaaag atgatcgaag ttaccgatac ggcagtcacc agttttgatg atgcagctga
     4141 aggcatcgaa caatcgagga ctgatggaaa aggcttaaat gaaagccacg ccaaaggatg
     4201 taagaattga tgcccaattc aaagaacaag cagacgagta tttagagatc atgtggtatg
     4261 aattccatga cgagtgatgg gaaaatggat tggcaaaatg ccttagcgga taagaaaaaa
     4321 acttagtcgg tggatgcaga tatgaacccg ttggcagtat gaagttggag tatacggaat
     4381 ggataaatag ctaagaaaag caactctttg cccataagga gggaagaatc agagtggatt
     4441 attgccttaa aaatgacaga actaaaaaag cctttgcgac caacaaatgg tcgcagaagc
     4501 ttttgttttt tacaataaca tcagcgtgat gtacggctgg ctggatctag ccgtctttcc
     4561 aaaataccta aaagccagtc cgtgatgatc gccattaatg cagttggcaa tgcacctgct
     4621 aaaataatcg atgtgccctc tgttgcattg gttcctcgga taatgatatc tccaagacca
     4681 ccagcaccga cgaatgcacc gatcgcggtt atcccgatgg caacaactaa cgcgtttctt
     4741 atgcctgcca tgataacaga aacagagagt ggtaactcta ccatgaatag tcgttgtcta
     4801 gcagtcatcc ccatgccttt gccgacatca aggatgtttt tgtctacttg gatcatcccg
     4861 gtatacgtat tcttgatgat cggtaagagg gaatacaaaa atacagtaac aatcacgaca
     4921 ttgactccca ggcctaatcc aatgattaaa atggagatca tggctagaga agggatcgtc
     4981 tggatgatat tcgccaaacg aatcacccag tttgctaatt tacgtctcct agaaatcatg
     5041 atcccaaccg gaatcccgac aactgctgca aagaggacac catagataga aatcaggaag
     5101 tgacggataa attgtgcaaa gatataggag ccattttcct ggaaataata gaaaaattgt
     5161 tgaaagacat tcattttatc caattgcttc acttcccttc aaaatagtgg ttttcttgta
     5221 agaaacgttg tgctactact gatggttcca ctagattatt atctgcttcg tagttcaatt
     5281 cctgcatttt ttccgtactg atggtatgac ctaacttatt taaggcttct tttaaatgag
     5341 gcgtatcagc taatatcttg ttgtctacaa ctggagcagc atcatatggt gggaagaatt
     5401 gacgatcatc ttccaacatg actagatcat aactggcgat ccgtccatcc gtagagtagc
     5461 caagaacaat atccattttt cctgcttcga ctgcgtcata aacaagtcca atctgcatag
     5521 gtaatatcga attgaaggag aagccataag tttcttggaa gccgtcataa ccgtctcctt
     5581 tacgattgat ccaagaagta tctacaccag ctactaattc atccgctaca tttttcagat
     5641 cgctgacttt agatagatgg tatttttgtg cggtgtcttt tcgaacaaga aagacatacg
     5701 tattttcaaa accataggac gggaaccacg tttgaccgta tcttttctca aattcatctt
     5761 tcacggtagc aaacgctttt ttcggatctt tttcaggagg aaggttcaat gtagtcgtta
     5821 aatctgttcc tgtgtagcga gccgctgaaa ttgaagcatc tccgttcatc atcgcttgat
     5881 ggttgatagt tgtagtagcc agattgttga tgattgcggt atttttatct gtataatgtt
     5941 caaccattcc ggcaactaga cttgctaaga tctgtgcttc tgaagtgatt ccacctgtga
     6001 tcgaaatggt gtcttcatct gtattgctgg caagaccagg gaaggaacaa ctcgcaagta
     6061 taagaagaga gaaaagaacc atggtcattt ttagtatacg tttcattctt cattctcctc
     6121 tctaagtgcg atcggtgtca gccggtgttc caagagacct agaagccaat cagccaatag
     6181 tgctaaaatt gttacaggga tcgtacctgc caaaatgaga tctggttggt aattattcag
     6241 tccactaaaa atcaaatcgc ctaaaccacc agcgccgata taagaggcaa gtgtagccca
     6301 tgcaatcacg taaacggcag ctaaacgaat accagccatg atcgttggca tagcaatggg
     6361 caattcaacg gaaaaaatgg attggaactc agtcatcccc atgccttttg ccacatcccg
     6421 ataattccaa tcgacatttt tcatcccaat gtacgtatta cgtaaaatcg gcaataatga
     6481 ataaataaag agtgcgataa tcgcaggtaa tttacctacg ccaaatagcg ggatcatcaa
     6541 tgccaataaa gcaagagaag ggattgtctg caaggcactg gttaagccaa tcacaatcgt
     6601 ggcagctttt ggaaaacgtg tcagtaagac accaagcggt acagcaatga tgataccaat
     6661 gatcaaagca agtcctgaaa tataaagatg ttcaaaacct tttgagagaa tttctgaacc
     6721 gtgctgatga aaaaactgct gcataggtca gacctcctta gattcttcag gagtagctga
     6781 ctctgcaaca tcagtagttg cgggatcttc tccccaaatc acatcgtaga cgatatccac
     6841 taatgatgca cgagtcagga tacctacgac tttgttctgt tcatcaacga ccggcacgta
     6901 tttcagcccg cgttttaaaa tgcgttgcaa ggtatcacga agataggcgg attcttttac
     6961 gtaaaaaaca tgtttattca aaatgtcgcc gacactggat acttttcctc gttttttatt
     7021 caacgtttct acatcgataa atccttttaa tacattcgaa ttatcggtaa ctaacaacgt
     7081 atctacacgt ttttccctca tcagtttgat tgcttcttgt aatgattttt ccggtgtaat
     7141 cgtgattgct gtatttagca taacttcgcc gactcttgtc gcatttggct tggcttgtag
     7201 taggcgatct tctccaatca actcttctac gaaatcgttt gcaggatgac gtaaaatatt
     7261 ttctggtgta tcaaattgga tcacacggcc ttcactcatg atggcgatac ggcttgctag
     7321 tttaacagaa ttaggcagaa gtcgcccatc ctctataggt gggtgatgaa tgccgttcgg
     7381 tatgagggtt accgttggta actcgaagac cgacaactaa ggcgtattgc ctttttattt
     7441 tgttccatgt tacaatcagt ctatacaaga aatgagttg
//
LOCUS       DAKBVG010000129         7346 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000129 DAKBVG010000000
VERSION     DAKBVG010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7346
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.129"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            95..1087
                     /locus_tag="OUD09_001930"
     CDS             95..1087
                     /locus_tag="OUD09_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCT9693191.1"
                     /translation="MCWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            1103..1987
                     /locus_tag="OUD09_001931"
     CDS             1103..1987
                     /locus_tag="OUD09_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCT9693192.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            2036..3643
                     /locus_tag="OUD09_001932"
     CDS             2036..3643
                     /locus_tag="OUD09_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCT9693193.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            3644..5128
                     /locus_tag="OUD09_001933"
     CDS             3644..5128
                     /locus_tag="OUD09_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HCT9693194.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            5305..6297
                     /locus_tag="OUD09_001934"
     CDS             5305..6297
                     /locus_tag="OUD09_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCT9693195.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            6349..7218
                     /locus_tag="OUD09_001935"
     CDS             6349..7218
                     /locus_tag="OUD09_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCT9693196.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
BASE COUNT         2334 a         1180 c         1629 g         2203 t
ORIGIN      
        1 atggtaatct ataaacgaaa atagtggtaa cgtttccaaa atgcatcttc gttaaatttt
       61 tattttttta aacgaaaatg gaaacgtttc taaaatgtgt tgggaggaga ttgaattgga
      121 aaagacattg ggaccaggtg gtgagataag ggcggtacaa cgggataaaa aaaatatttc
      181 gaagtgggaa aaattaaaga aacagttgcg gcgtgataag tggatgtact tgttgctctt
      241 accaggtatc atcatgacgt ttatctttaa gtatgtgcca atgtacggtg ctgtgattgc
      301 atttaaagac tataatccta tgctaggaat tttggaaagt ccttgggtag gactagatca
      361 ctttagggaa tttatcagtt caccaaattt tacgcttttg ctgagcaaca cattaaaaat
      421 tagtatttat ggtttattgt taggtttttt tccaccaatt attttagcac tgagcttgaa
      481 tcaaattatc aataaaaaaa taaaacagaa aatccagttg attctatatg cgccgaattt
      541 tatctcagta gttattattg tggggatgct gtttttgttc ctatccgcaa atggacctat
      601 taatgggtta gtagaaaagg ttacggggca accaattatg ttcatgtcta atccagctta
      661 tttcagaacg atttatattt tatcgggaat ttggcaagga atcggctggt catccattct
      721 ttacaccgca acgctggcgg gagtcagtac cgatttatat gaggcagcaa atatcgatgg
      781 ggcaaatatt ttacaaaaga tttggcatat cgatttgcct gcattgaggc ctgtgatggt
      841 gatcagcttc atcttggccg ccggcggtat tatgaatgta ggatatgaaa aagcctactt
      901 gatgcaaact tcaatgaaca tccctgcctc agaaatcatt tccacttatg tatacaaagt
      961 cggcttgcaa tcaggtgatt atgcctactc tgctgctgtt ggtctgttca atatgattat
     1021 taatgtcgtt ttattactga ttgtcaacaa tataaccaag cggctaaacg atggtcaagg
     1081 cttgtaatga aaggagaaaa ttatgaatag tgctttatca aaaatcgacc gtcaaatctt
     1141 atggttgaat cgagtgattg taactttttt agtcgtcatc accattgttc cgctgattta
     1201 tgtactggta gcttctttta tggacccagc tactttgatc aataaaggta taagcttcaa
     1261 tccgaaagat tggacaatac agggatatca acgggtattt gcagatgatt ccattttacg
     1321 cggttttgta aattcgttgt tttactcttt tagcttcagc ttattgactg tatttattac
     1381 gatgatcact gcgtatcctt tgtctcgacc taacttggcg gggaaaaaga ttattatgac
     1441 tttttttgtc attacaatgt tcgtgggtgg tggcttgata cctacctatt tattggtaaa
     1501 gaacttgggc atgttagata gtgtatgggc gattatcgta ccaggtgcta taaatgtgtg
     1561 gaatattatc ttagcccgta cctattttag tgctttgcca aaagaacttt ctgaagctgc
     1621 tactatcgat ggcgccaatg acttgcaaat actgttcaaa gtcatgcttc cattagcgaa
     1681 accaattatg ttcgtattgt ttttatatgc ctttgttggg caatggaact catactttga
     1741 tgccatgatt tacatcaaag atcccgactt acagccgttg caactcgtat taagaaatat
     1801 tttgatccaa aaccaacctt cacaagacat ggtgggagcc aatactgcca tggcagaaat
     1861 gaaacaaatt gctgagttga tcaaatacgc aacgatcgtc atttctagtc tgccattaat
     1921 cataatgtat cctttcttcc aaaaatattt tgataaaggt gttttagttg gttctgttaa
     1981 agggtaagta ttgttcacta tttgtatgaa taaaaattat aaaggagatt ttaagatgaa
     2041 agcaaaatgg atgctgccaa tcgtttttac ttccgtacta attttggggg cttgtggagg
     2101 ctcaaaagaa aagagtaaat caagccctga ttatgaacta gaaaatgtct cttttccatt
     2161 gaaagaaaaa gtatctttaa aaatgatgtc tcaaagtgcc cctttggctc ccaatgatcc
     2221 taatgacaaa ttgattttcc aacgattaga agaagagacc aatctccata ttgattggac
     2281 aaactacagt agtgattttg ccgaaaaaag aaatttggat atcgctagtg gtgatttacc
     2341 agatgccatc ttcaacgccg ctgcaggtga ctatgattta ttaaactggg cggaaagtgg
     2401 cgttattgta ccgattgaag atttgatcga agattatatg ccaaatctga agaaaatttt
     2461 tgatgaaaac cccgaatacc gacaaatgtc tactgcacct gatggtcaca tttactctat
     2521 gccttggatt gaagaattag gcgaaggaaa agagtcgatt catacagtaa atgggatggc
     2581 ttggatcaac aaggagtggt tggataattt gggtcttgaa atgccgcaaa ccactgatca
     2641 gttgatgact gtgttggagg ctttcaagac gcaagatcca aatggtaatg gtgaagcaga
     2701 tgaaattcct tttagcttta ttaacaatgg aggaaacgaa gacttaaaat ttttaatggg
     2761 ggcattcggt ctaggtgata atgatgacca cttagtcgtt gataacgatg gtcaggtaca
     2821 atttactgct aacaaagaag ggtataaaga agccatcaaa tatttcaatg aaatgaataa
     2881 taaaggtctt attgataaag aggctttcga acaagattgg aatacgtatg ttgccaaagg
     2941 gaaagaacat aagtatggcg tgtactttac ttgggataaa gcaaatatca cgggtgcaga
     3001 tgatagttat gacgtcttgc cagtcttagc gggaccagat ggaacgaaac atgttactcg
     3061 tacgaatggc atgggtttct caagagatcg tttcgtaatt actagtgcga ataaaaactt
     3121 ggaacttaca gctaggtgga tagataaaat gtatgaaccg ttacagtctg tccaaaataa
     3181 ctggggaacc tacggtgatg aagcacaggc gaatattttt gaattaaagg atggtatgtt
     3241 aaaacatttg cctttagagg ggacagcacc gggggaacta agacaaaaaa ccgaagctgg
     3301 aggaccatta gcagttcttg attcttatta cggcacagtc actactatgc cagatgatgc
     3361 gaagtggcgt ttagatttga tgcatgacac gtatttgcca tatattacag aagacaatat
     3421 ctatccacgt gtctttttag aaaaagaaga aacagatcga ttagcaaaaa tcgaaacgga
     3481 tatgtgggat tacatctatc gcaaaagagc agagtggatc accggcggta aaatcgatga
     3541 agaatgggaa gaataccttg ctgaattgga acgtgtaggt ttggaagaat ggattcaaat
     3601 aaaacaaact ggctatgata attttttgaa gggagcacaa taagtgttag tagaagaaac
     3661 ggcgattttg gataaagcaa atcgatttat tgaaatgatg cgaggaaaag ttaaccctca
     3721 gtatcgacaa aactatcatt tttccgcacc catcgggtgg ataaatgacc ccaatgggtt
     3781 tatatattac aaagggcatt accatctctt ctatcaatac tatccttacg atagtcgatg
     3841 ggggcccatg cattgggggc atgcgcgcag tagtgatttg gtccattggg aagatctacc
     3901 agtagcgtta gccccagatc aaccttatga cagcggcggg tgtttctcag gaagtgcaat
     3961 tgaaagagac ggaaaactat atttatttta tacaggctgt caagaaattg atggtattag
     4021 ccatcagcat caatgtttgg cggtctctga agatggtatt cattttagca aatacagtgg
     4081 taatcctgtg gtagcagaaa aacagctagg tgaaaatggg ctaatacaag aatttcgtga
     4141 tcctaaagta attgttcaca acgatggtta ctatatggta gtagctacta aatctacaca
     4201 gtctactggt agaatagtga tgtttcaatc gtcagatttg tatacttggc acttttactc
     4261 tgtgctgcta gaagggactt cagagcaagg aataatgtgg gaatgtcctg atttattcca
     4321 tttagacgga cgagatgtct taatcatgtc tccgatacaa atcaagaaga acggactgga
     4381 atatcataat atcagttcta ccatggcttg tattggtgag gtaaactggg aaacaggtca
     4441 gatgatagtt gataatttcc atgaaatgga ttttggaatg gatttttatg caccacaaac
     4501 aacaagagat cctaatggtg gacgggtgat gattgcttgg atgcagatgt gggatcgtac
     4561 attgcctaca catgagttag gccatctttg ggcaggctct atgagtttgc cccgagagtt
     4621 aagagtgaaa aaaaatcgat tggtacaaaa acccatctca actgtatata gtcatttagt
     4681 ttatcaacaa ggcagtgaaa atattgcagt gactgacaca tcagtcgtat ttcgaaacgc
     4741 gctgtctgat tcttcatatt tacatctagt cgcagatttg gagaaagctg aagtttttac
     4801 aattgaatta ctgcggtcta agacggattg tctcaaacta gcctatgaca ctgctacaca
     4861 ggtttttgaa ttggatcgtc agaaactagg aagagagctt acaggggcgg aagaagaccc
     4921 tctgaatgta cgaaaagttg aagtaccttt gattgataat cagctcgttc ttgagatttt
     4981 tagagatacc tcatcaattg aagtctttat taatgggtta gaagttatga cggctacttt
     5041 ctatgagctg gaaaaagctc gagatatctt gtttcattcg atcggagaaa gtcgtattca
     5101 agcttttgaa acagcgagtg tgaaataaat gtttgattgc ttaggcgatg aatcaattat
     5161 ttaataggtt ttgaaggata atgtttttag aaaagaacag atagctagat ttcccatgga
     5221 tttgctatta gtattttgaa aaaattagta atttatgaca aaattcatta tactaaactt
     5281 gtgcagaaat gtgagggatc aactatgaaa gcaacaatga aagatgtcgc aaaattagca
     5341 ggtgttggag taggaactgt ttcccgggta ttgaataacg gttctgttaa agcttctact
     5401 agagaaaaag ttgaagcagc aatgacagag ttaaactatc aaccagattt ttatgcccgg
     5461 gggttaaaga tgaagcatac gaacacggtt gctttgattc tacccacggt atggcatcca
     5521 ttttttggcg aatttgccta ccatgtagaa acagcattag aaagacaaaa ctacaagctt
     5581 tatttgtgta actccagtgg taatagtgaa aaagaacaag aatatatcca gatggtcagc
     5641 caaaataaag tagacggcat cattggaatt acttattcag atatcgatga atacatttcg
     5701 agtaatctgc cttttgttag tattgaccgt tatttcactg aagaggtgat ttatgtgacc
     5761 gctgacaatg cggccggtgg acgtttggct gcaaatgaac tttatcgccg aggatgtcag
     5821 aatgtagcat atatcggtgg gtaccaagaa acaccgaatg aaacgaagag tcgtcgaaaa
     5881 tattttgaaa tcgagtgccg tcaactgggg atttcctata aagcgttaga tatgttagaa
     5941 cctttagacc atctctcaga acgtgtaaca aattttttgt tagagaatcc cgatatcgat
     6001 gggattttta cgatcaacga tccaatggca ttagctgtca ttgatatgct tgaaaaaata
     6061 gggaaaaacg taccagagga tgtccaagtt atcggatttg atggtcaaaa gatgacagaa
     6121 ggagatcctt atctagtctc aacgattgcc caacctacaa agcaaatggc ggaagtggca
     6181 gtggcaaaat taattgctgt cattaatgga gagacagtag aaaaacgtac cgtattgccg
     6241 attgaatttg gcgaaggtga cacaacgaaa cctttagtga gaaacgattt tgcttagtgc
     6301 aaatacgttt ctttttttga aaatgagtga atgaaaggtg tagataaaat gttatatggt
     6361 ggaatagaag ccggtggaac aaagtttgtc tgtgggattg gtaatgaaca attacaagtg
     6421 atagaacgga aaagttttcc taccacaaca ccagaagaaa cgatggagca agtgtttgat
     6481 ttctttgata agtacaaagg aaatatcgca tcaattggtg taggagcatt tgggcctatt
     6541 gatattcaaa tcgaatcaag aacttacgga tatatcacca acacaccgaa gttagcttgg
     6601 caaaattatc attttgtagg agctttggaa aacgccttgt cggttcccat ctattggact
     6661 accgatgtaa atgctgcttg ttatggagaa tatatggcag ggtatggaaa aggtaaaaat
     6721 agtgttctgt attacaccgt tggtacaggt gttggtggtg gtgctgtagt ggatgggaaa
     6781 atagtcgcag gttttagtca tcctgaaatg ggacatatgc tcttacaacg tcataatgaa
     6841 gatcaatttg aaggaatctg tccttttcat catgattgtt tggaagggtt agcatcagga
     6901 cctgcgattg aaaaacgctt tggaagaaaa gcccagacta tctcatttga tcatccttat
     6961 tggacgattg aggcagatta tcttgctcag tgtgtgtata acacaacgtt actatatgca
     7021 ccggaagtaa taattttagg cgggggtgta atgaaacagc cgcaactact cgagttgatt
     7081 cgccaatcgt ttaaagaaaa aatgggttcc tacgtggata cgcctactct cgaagaatat
     7141 ctactaactc caaggcttgc tgacaatgcg ggcttgattg gctgcctagc tttagcaata
     7201 aagaaactca atcattagaa tagtatttat tagaactatt cataacatac cggaagttta
     7261 aaagggtatt ctgaaactta aatagtctca attcattctt ctttcttgag aatctgagta
     7321 gagactttta ttctgtttca aattaa
//
LOCUS       DAKBVG010000130         7300 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000130 DAKBVG010000000
VERSION     DAKBVG010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7300
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.130"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(203..1651)
                     /gene="cls"
                     /locus_tag="OUD09_001936"
     CDS             complement(203..1651)
                     /gene="cls"
                     /locus_tag="OUD09_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCT9693197.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEIAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYILQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLIYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            complement(1803..2696)
                     /locus_tag="OUD09_001937"
     CDS             complement(1803..2696)
                     /locus_tag="OUD09_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT9693198.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            complement(2680..3366)
                     /gene="ftsE"
                     /locus_tag="OUD09_001938"
     CDS             complement(2680..3366)
                     /gene="ftsE"
                     /locus_tag="OUD09_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCT9693199.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            complement(3471..4572)
                     /gene="prfB"
                     /locus_tag="OUD09_001939"
     CDS             complement(join(3471..4499,4501..4572))
                     /gene="prfB"
                     /locus_tag="OUD09_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCT9693200.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            complement(4698..7232)
                     /gene="secA"
                     /locus_tag="OUD09_001940"
     CDS             complement(4698..7232)
                     /gene="secA"
                     /locus_tag="OUD09_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCT9693201.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
BASE COUNT         1937 a         1629 c         1277 g         2457 t
ORIGIN      
        1 ctatactggc tttgaaatgt gtctctttta atagcatata ttagcttatt tcttgctttc
       61 cccaaaaaac aagagatacc ccaaagttaa tttcgctcat gcgagacgct ttctccattt
      121 ctgacgtact ctcagaaatg gccccgttag aaaaggacac gagatctcca tctgttgatg
      181 gttctcatgt cctttttgtg ttttaaagga gcggcgaaat caatcttgct aatccttctt
      241 tcatcttgat gattattcct cggttttcat acatttcttt tgtgagatgc gtagaaattt
      301 tcgagtcttc aaaaaatgct tttcttacac gtgaagccat tcgttcatca taaatcagtg
      361 tattgacctc aaagtccaag cgaaatgaac gaacatcgat atttgctgag ccaactgaaa
      421 caatgccacc atcgataatc atcgtctttg catgaataaa tccattctca tatgtttcga
      481 tccttgctcc atactcgatc agctctgcag cgaaagaata cgttgcccag taaacgagaa
      541 gatgatctgg tttgttaggt atttggatat gaacttttac gcctgaaaga agagccaact
      601 tcagtgcttc atgaatcgat ccatccggta tgtaataagg cgtttggatc aatatctccc
      661 gtttggctaa cgaaatcatt ttcaaataag tcattttgat ttgttcatgt tcggtatctg
      721 gtccgcttgt cacgatttga acagctatat caccagtaga gtgaatggac ggaaaaaatt
      781 tgggagaata gcctacttct tttgtatgct gagaattcca gtccatcaaa aaacggtttt
      841 gcaagatata caccgcttct ccgtaaatac gcaaatgatt gtctcgccaa taaccaaatt
      901 tctttaccaa tcctagatat tcatttccaa cattgaaccc acctgtatat ccgattttcc
      961 catcaataac tacgatttta cgatgattgc gataattgat ccgaggatta atatacggaa
     1021 caaatagagg aaagaaaaat gcgatttctc cgcctgcttt tttcaaattc tgaaaaaagc
     1081 tggaagaaac ttgtgtggaa ccccatgcat caagaaggac tctgactttt acccctcgtc
     1141 ttgccgcctc tgtcaatgca tctcgaactt cttcacctaa tgcatcactg cgataaatat
     1201 aatactggaa gtggatatgg tcttctgctt gcttgatatc ttcaatcagc gcatcgaatt
     1261 tttcacgacc atctgtaaat aaagtgattt catttttagt cgtatagagc gtactttcaa
     1321 ataccgtgag catatagatc aattgtttca catctacttg tccagtaggc ggatgaggat
     1381 aaagccctcg catcaatgcc tgtttgtctt cttcaatttc caggttctta ccgattttcc
     1441 cttggatctt caaatcaaag attttctctt tggaaatccc tcttcctaaa aaaatatata
     1501 aaataaatcc tacaactgga atgaacatca aaacgagcag ccatgcccat gtttgagcag
     1561 tttcctttct ttctcgaaaa acgatgataa aggaaagcaa aatattcata atcaaaagga
     1621 tcgtaaaaaa attgtcaacg ataattttca cggcttcacc tcttcctcta ccaacttaag
     1681 aatacccaag ttgtttttta aagtaaaggg aaaaaagccg cttccttcta ttctcctagc
     1741 caataaaaaa agaaagctgg aacgaaactg tccaactttc tcattttatt tatattgtgg
     1801 tattagaatt tcaagaaacg tctcatagaa atgatcgagc caagtgaacc aatgatcaca
     1861 ccaatgccga ccattaaaag attgattttc caaacgatat tttgtggatg aatcaatgaa
     1921 taatgggaac gtaataatga tggattgatc aattggaaaa accatgcata accaaatgtg
     1981 atgatcaaaa tcggaagaat cgcaccgatc agaccgatcc agccaccttc aaggaaaaac
     2041 ggccagcgaa tatatccatt ttttgcaccg actaaacgca tgatttgaat ttcacgctgt
     2101 ctagacaaaa tcgtgatccg aatcgtgttc gaaatgagaa acatcgctac gaacagcagc
     2161 aatgccgctg caactaatcc ccatgtccga actgcaccag cgatttggaa aatccgatct
     2221 gaggaagctc caccgtaatc agaacgagaa acattcttca aatctgctgc ttcttctgct
     2281 acttgttttg tataagaagg atcagtagca cttactacat acacatcata taacgggtta
     2341 ttgtcacctt caaacaattt ccaggaatca cccatttcgt tttggatttt tttcaattcc
     2401 tgttctttac ttgaaaattc aactttttta acatgatcta agtcattcaa ttctttttct
     2461 agctttttca tctcttcgtc ttttgtaccg atatctacga atacagatac atcaacgttt
     2521 ccttctatat cctgagctaa tttagttgca ttcatgataa ctgccatgaa tacaccgact
     2581 aaagtcaacg tgatcgttac cgcactgact gaagcaactg tcatccagcc gttacgtttc
     2641 aaactcttga tactttctaa taaatggcgg aaaaaggttc taatcatcgt atccatattc
     2701 cccttccgct tgatctcgaa taatgcggcc gttttcgata gcaattactc ggtgccgaat
     2761 cgtatttacg atcgtactgt tatgtgtagc catcacgaca gtagttcctt gggcattgat
     2821 ccgatctaat aatttcatga tttcccatga gttttctgga tccagatttc ctgtcggttc
     2881 atccgcgata agtactttag gcgtattcac gattgcccga gcaatagaga cacgttgctg
     2941 ttccccaccg gataattcgc taggaaagac tcgaactttg tgtttcaaac caacaaggtc
     3001 cagcacttcc attactcgtt ttttgatatc tcgaggtttt ctgccgataa cttgcatagc
     3061 gtacgcaaca ttttcatata ctgtttttct tggtaataat ttatagtctt ggaacaccac
     3121 gccgatttct ctgcgtagat aaggaacttc gcggtttttg attgctatta gatcatgacc
     3181 tgcgacgttc aaagttcctt ttgaagcttt ttcttctcga tacatcaatt tgatgaatgt
     3241 agatttaccg gctccagacg gaccaaccac atagacaaac tctccttgtt cgatgacaat
     3301 cgagagattg cggatagcgg tcgtaccatt tgagtacttc ttcattacat ccttcatttc
     3361 aatcatggcg ttctctccat atctttttca gtctttagct attatagcat ggcaatgttt
     3421 caacatgctt gcaaaatctt acaattttat ttcaatttaa caatgggttc ttattgtaga
     3481 tgcattttta aatagccgtc aataaataaa tcaaggtcac cgtccataac tgcttggaca
     3541 tttcctgtct ctacgtttgt ccgatggtct tttaccatag aatagggatg gaaaacataa
     3601 gaacggatct gtgaacccca gccgatttct ttttgttctc ctcgaagtgc agctgcttct
     3661 tgttctttct tttccacttc taattgataa agcttagctt ttaacatccc catcgcttgc
     3721 tcacggtttt tcaattgtga gcgttgtgcc tgactagcta ctactgttcc tgtaggcaag
     3781 tgagtgatcc gtacagcaga ttctgtttta ttgatgtgct gaccacctgc accacttgct
     3841 cggtacgtat cgattttcaa atcatctgga ttgatttcta tatcgatcgt ttcatcaagc
     3901 tctggcatca catctaccga acaaaaagaa gtatgacgtc gtttcgctga gtcaaaaggc
     3961 gaaatccgta caaggcgatg aacacctttt tctgatttta gataaccaaa agcgttatgt
     4021 cctttaatca gcagggtcac actcttgatc cctgcttcat cacctgcttg ataatctagt
     4081 gtttctactt gatagccatg ctgctctgcc caacgtgtat acatacgtaa aagcatgctg
     4141 ccccagtctt gggactccgt tccgccagcc cctggatgca attcgacgat cgcattgttt
     4201 cgatcataag gtccgtctaa cagcatagat aattcatacg tcttcatttt ttcatctaat
     4261 gcattgatac gtgtttctgc ttcttcttgc attgctgcat caggttcttc ttgcagcatc
     4321 tccaccatca attcgatttc ttctagttct tcggcaagag aatggaattg gtcataggtt
     4381 tctttattag cgttattttc actgatcact ttttgtgcgg cttcgctgtc atcccaaaaa
     4441 ccaggttcag ccatttttgc ttctgcttcc gcgatgtctt cttccagctg atctaagtca
     4501 aagagacctc ctgaaacttg tgaccttttc ctgcatttct gtcaggagat ttcgaatttc
     4561 ggatatttcc atgttttttc cttctttcac tatacttttt tcattatatc tagtctaaca
     4621 gaatcagcaa ataacagaaa gaaggtgaga cgaattctta aaaaaagaat ttgtctcacc
     4681 aatcctgctg tcagaaatta gtttgcattt tttccatgac agttcttata ttttttcccg
     4741 cttccacaag gacaaggatc atttcttccg accttgttca catggattgg ctgttttttc
     4801 gcgctagtat tactattttc tttggcatct ccgtcttctg agtgagaagc tgttccttga
     4861 gaaacctgtt cacgctgtac gttttgacgg atctcagctt tcataaagat tcgagtgact
     4921 tcatactcga tggcgcctac catatcttca aacatcttat agccttctgt ttgatattcg
     4981 actaatggat tgttctgtcc ataagcacgt aagccgattg actgacgtaa ttgatccatt
     5041 gcgtcaatat ggtctgtcca tttagagtcc acaacacgca agatcacgac tttttcaaat
     5101 tccaacagtt gctctggacc atttagttga gccgctttgg tattaaatac ttcttgtgct
     5161 cgttcgttta gatactcttt gatctcctga gctgtttttc cttcgatatc tgcaagagta
     5221 atactatctt catggacaag ggtactgccg gcaaaatcga tgatactttc caaattccaa
     5281 ttttctttct ctaattgtgt atgcgcatct actacacgac tgattgtccg tttgaccatg
     5341 ttcattagtg gcttggataa gcttttttct tccatgatta cttcttgtcg ctgtttatag
     5401 attacttcac gttgttcacg catcacatca tcgtattgta agacgttttt acgtgtgtca
     5461 tagttatttc cttctacacg tttttgtgca gattctactt gtttcgtcag catcttgctt
     5521 tggataacag catcttcctc accgatcttc atccgatcta agaaagcttt gattcgttct
     5581 gaaccgaatc gtttcatcaa atcatcttcc aatgacaagt agaactgtga cataccaggg
     5641 tctccttgac gtcctgcacg tcctcgaagc tggttatcga tacgacgaga ttcatgtcgt
     5701 tctgtcccga taactgctag accaccaagt tctcttaccc caagtcctaa cttgatatct
     5761 gttccccgac cagccatgtt ggtagcaatc gtcacagcac ctttttgacc agcattcatg
     5821 atgatctctg cttctttaaa gtgatttttc gcattcagca cttcatgagg aatacgttct
     5881 tgattcagca tatctgataa taactcagat gtttcaaccg caacagttcc gactaacaca
     5941 ggttgacctt tgcggtagcg atctttgata tcttgcacga cagcgtggaa cttgctttgt
     6001 aatgttgggt aaagcaaatc tggacgatca tcacggataa ccggacgatt tgtcggaatc
     6061 tggtatactt ggatgttata aatttctctg aattcttctt cttctgtttt agctgtacca
     6121 gtcattcccg caagtttctt gtacatacgg aaatagttct ggaaagtgat cgtcgccatg
     6181 gttttcgttt cgtcttcgat ctctacgcct tctttagctt cgattgcttg gtgaagtcca
     6241 tctgaataac ggcgtccatc catgatccga ccagtgaact ggtcaacaat cagtactttt
     6301 ccgtcttgga caacataatc gatatctaaa agcatgatat agttggctct caatgcttga
     6361 tccaagtgat gggtcaatgc agtattttca atgtcgtata agttatccaa accgaatgtt
     6421 tcttctgctt tttcgatccc tgcttcggtt aaactgattg tttttgattg tacatcgatt
     6481 ttataatctt cttcttcttt caaacgtttc acgaagttgt ctgctcttgt atataaagct
     6541 gtggattttt ctgcttgccc tgaaatgatc agcggcgttc tcgcttcatc aatcaagatt
     6601 gagtcaactt catcgacaat ggcataattc agcggacgtt gtaccatttg atggcggtaa
     6661 acgaccatat tgtctctcaa gtagtcaaat cctaattcat tattcgtact ataagtgata
     6721 tcacaattat aagcctcgcg tttttcatcc gaagattttg agttgatatt caaccccact
     6781 gttaatccta agaaattata aagttcgccc atttccgtcg agtcacgggt tgctaaatat
     6841 tcattgacgg taactacgtg taccccttcg ccagtcagag catttagata tactggcatc
     6901 gttgcggtca aggttttacc ttcaccagta cgcatttcag ggatatttcc atcgtgaagc
     6961 acgattccac ccatcaattg tacgtggtat ggatataaac ctaatacacg ttttgctgct
     7021 tctcggacga ctgcaaaagc ttccggaagt aattgatcta atgtttctcc tttttggtat
     7081 ctgcccttga attcagcagt ttttgcctgt aattgttcat cagttaatgc tgccatttgg
     7141 tcagcgtatg cttcaacttt ttttgcaatg ctatctaaac gttttagttc tttcttatcg
     7201 ttttcgataa gtgaacgaat caaattggcc atgcgttgtg taatctcctt ttccttctgt
     7261 gtattatcca gactatgtag actttacttt tcattttatc
//
LOCUS       DAKBVG010000131         7256 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000131 DAKBVG010000000
VERSION     DAKBVG010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7256
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.131"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            108..1529
                     /gene="arcD"
                     /locus_tag="OUD09_001941"
     CDS             108..1529
                     /gene="arcD"
                     /locus_tag="OUD09_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HCT9693202.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFAIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVAFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            1543..2661
                     /locus_tag="OUD09_001942"
     CDS             1543..2661
                     /locus_tag="OUD09_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HCT9693203.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SEFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILER
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(2721..4013)
                     /locus_tag="OUD09_001943"
     CDS             complement(2721..4013)
                     /locus_tag="OUD09_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HCT9693204.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLAHQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNAAALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLMGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSISSGHSPSHRQ"
     gene            4145..4723
                     /locus_tag="OUD09_001944"
     CDS             4145..4723
                     /locus_tag="OUD09_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCT9693205.1"
                     /translation="MRGNMKMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLEGIHETNQIILQIFEAFQLTKEEKVHRSRELCSMIHGYL
                     SLRFLGYFTKESTIDPEDSYFWMINDFIATLP"
     gene            4852..5229
                     /locus_tag="OUD09_001945"
     CDS             4852..5229
                     /locus_tag="OUD09_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HCT9693206.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEAEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESGKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            5162..7234
                     /locus_tag="OUD09_001946"
     CDS             5162..7234
                     /locus_tag="OUD09_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCT9693207.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEEQKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKEMRINPDF
                     TIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKMLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
BASE COUNT         2427 a         1332 c         1562 g         1935 t
ORIGIN      
        1 tccaataata tgaataaagt tgctttttgt taagcaagag cagttgcaaa atagaaggaa
       61 atcttttgca agtgatttgt tcaattttat aagtgagggt gatggaaatg gaaaagaaat
      121 caacgggtat tagcaaatgg ggactggttg ctctggttgt cagttcgtca atcggaagcg
      181 gtgtgtttgg gatcacaagt gatctggcta gttctgcagc tccagggcca gcgatccttt
      241 catggataat cgttgggatt ggtattttag ctttggtatt atcgctgaat catttaggag
      301 aaaaacgtcc agatctagat ggtggtattt tcggttacgc ggaagcttca tttggtaaat
      361 taggtggctt catcagtggt tggggttact ggttgtctgc gtggctggga aatgttgctt
      421 ttgcaacgat gctgatgagc gccatgggag aatttttcgc gatattcaaa ggtggacaaa
      481 atgttccttc tattttattt gcaagtattt ttatttgggg tctgacttta ttagtaaaca
      541 atggagttga aggagcgagc gtcatcaata caatcgtgac gatctgtaaa ttagtgccat
      601 tattcctatt tttaatattt gtatttatcg catttaaagt gaatatattc actgctgatt
      661 tttggggtaa cgtatctgat aatctagtga atggaacagg gcagcaagga acgatctggt
      721 tgcagattaa aggttgtttg atggttatga tgtgggtctt tgtagggatc gaaggcgcaa
      781 gtgttttagc caaccgcgcg aagaaacgtt cagaagccca gcaagcatcg atcattggtc
      841 tgctttgttt attgtttatc tatatccttg cttcgatctt gccatacggc gtcttatctc
      901 aagaagagtt agcaactatt tcacaacctg ctatggcaaa tattttaaaa ggaatcgtgg
      961 gaaattgggg agcagcgctg atcaatatcg gcttgattat ttcgatcatt ggttcttggt
     1021 tgtcttggac gatgctccca gcagaaacaa cgatgttgat ggctcgagac ggtctgttgc
     1081 cgaaaggttg gggaaaaacg aataaaaaaa atgccccaac ttattcgtta gttgttactg
     1141 caatcttgac taatctattc ttattgactt tcttagttac tgattatgcc tatcaatttg
     1201 cctattcttt atgtacagcg gcaattttga tttgttatct acttgtcggg atctatcaag
     1261 tccagttttc tttaaaagaa ggaatcaaaa aacaagtagt gatcggtggg attgctgtag
     1321 cttttgaatt gatgggcatc attttagcag gattttctta tgtcctttta tgtagtattg
     1381 cctatcttcc tggtttttgc ttctactgga tcgcttgtcg tgaaaccaaa catcaaatta
     1441 cgggaaaaga aaaagtaatg attgggctga tttgtgtggc cgcactgctt tcaatcgggt
     1501 tgcttgctgt aggctggatc aagatataag ggggaaagaa acatgaaaat cgcaaatttc
     1561 ggtgtagaag aatggctaaa taaatgggaa aagaaagcgg tatatgatat tgcacaaagc
     1621 tcgatcgaag cattgacatt agacgaactc gttggcttag atgggactag cgtaagtgaa
     1681 tttttcgaaa aacgcaaaaa cgaaccattg gactatggct ggatcgaagg atcagatgtt
     1741 ttcaaacaag aagtcgcttc tctttatcaa accgtcgatc cggaaaacat cttacaaacg
     1801 aatggagcga ctggtgctaa tcttttagct ctatacgcgt tagtggaagc aggagaccat
     1861 gttgtttcga tgcttcctac ttatcagcaa ttatatgaca tccccaaatc tttaggtgcc
     1921 gctgttgact ttgttcattt gaaggaagaa gaagattggc agtttgattt agaacagcta
     1981 gaagccttag tcaaacctga aacaaaaatc atctgtttga atagtgcaaa caatcctaca
     2041 ggcacattgc tagatcggaa aactttagaa aaaatcgcag aaattgctcg tacagtagat
     2101 gcatatgtat tgatagatga agtatatgcg cctttaacgg ataaaggtga attcttgtcg
     2161 atcgtagatg tctatgaaaa aggtatagca accaattccc tatccaaaac ctactccatt
     2221 ccaggtattc gtattggctg gacagctact ggacctgagt tggcagaagt ttttcgaaag
     2281 tatcgagact acacgatgat ttgcggcgga gtcctgtcag atgatcttgc tgtacatgca
     2341 ttgaagaata aagaaaaaat attggagcga aacaaaaaga tcattacaga gaaccttgtg
     2401 attttaaaac aatgggtagc caatgaaccg aaagttgaac ttgtcgctcc caattacgtc
     2461 tctacttctt tcattaaatt agccatcgaa gaagatgacc agacattttg tatcaattta
     2521 ttagaagaaa caggagtact attagtacca ggaagtgcct ttgatttacc gaagcatgcc
     2581 cgactaggtt actgttgtaa aaaagaaacc ttggaaaagg gattattcct cttatctgag
     2641 ttcttgaaaa aacaggccta aacaaaaaca ctgcaaggac agtctgtgac aactgttcaa
     2701 gcagtgtttt tcatttacgc ttattgtcta tgagaaggag agtgccctga agatatggag
     2761 actcctgaag ctttttcttc tgcagtttct tgctgatgga tctcttgtgt attgtgtcgt
     2821 acaagcaaga agttgaccaa aacaagaaga gaagtcgaaa gaaagacgcc acgataacca
     2881 aatgcactag aaacactgga gccgatcata ggtcctacta cgttaccagt tgcttggaaa
     2941 gattggttat aactgaagat ccgtccagca gcttgatgag gagaatattt tgtgatcaat
     3001 gcttggacag cagggagcag acaagcatca gagataccca tcaaaaaacg taaggcagct
     3061 aattgccaaa cattcgtgac gaatgccatt gggatataaa ccagaatcgc aaaacccaaa
     3121 ccaatcgcaa gtattctttc actcccaatc ttgtcaccta agcgaccaaa tcttggagcg
     3181 gctatcaaag tggcaatccc aggtatcgaa gcgatgattc cgctgatcaa cgtaacattc
     3241 ccatgtccat gcagcaattg gcgaatgtac agactgatga ttggactaat ggaattgttg
     3301 gatgcttgga tgatcatcgt tgtgacgaac ataccgatga cgacgtgtgg atatttcaag
     3361 tctttgaaaa tctgtttggc agaaaccatg ttttcttttt tcactgggac aaatttttca
     3421 tggacgaaaa acagactcaa caagaagacg aaaaataaga tgatccctgt aatgaaaaag
     3481 gtagggcgat agccaaacgc tgaagcagag atcccgccaa gaagcggtcc aagtaatgtt
     3541 cctgtaacag aaccagttgc taaagttcca agaacttgac cacttttttc ttttggcgtc
     3601 ccagttgcga caagggcagc ggcattactg atataaccag aaaaaatccc ttgaagcaga
     3661 cgcaatgcga ctaattgata cacactagtc acgcaaccca tcaaagaaat gacgattgcc
     3721 attcctaatg aagcacgcaa cagcatcaat tttcttcctt tttggtgggc taattttccc
     3781 caccaaggag agatcagcgt agtcaccaag aaagtagatg aaaaagtcaa tccgctccaa
     3841 aagttcaatt gtgacgtact ataattacct aacgtatcta tgtaaagtga cataaaaggc
     3901 atgactaaac taaatccgat accagccata aatgttccaa accataagac aaacaagttt
     3961 tgttcccaag tttctctttt cctaaataat ttttccttta ttcttgcagc caattagact
     4021 ccttctttct gaaaattttc atctacagct ttttgttata aactacaata tgtagtttat
     4081 caatgaaagt ctcttttttc tctttctttt tcgtggtaaa ctgaaagagt gaaggattta
     4141 ttttatgaga ggaaacatga aaatgaaaaa aagaaatctg tctcaagaga aaatcatcga
     4201 ctgctttcga gaactagcag aagagatgga tgttcagcaa ataacctttc agcatttagc
     4261 aaaacgcttg gatatcaagg ctccctcgct gtataaccat ttcaaaaata ttcgtgatgt
     4321 caaaacagcg ctaacagcga aacttctgca ggaacttaat gaccagctgc gtcgggctct
     4381 tgtgggaaaa agtggtcagg aagcactgca gatctatgca gaaacttatc gatcctttgc
     4441 tttttcaaat caagcagtgt atgagttact gatcagtgtg ccccacacca atgaagcagt
     4501 tttattagag ggaatccatg aaacgaacca gatcattttg cagatctttg aggcttttca
     4561 attaacgaaa gaagaaaaag tccaccgaag cagagaatta tgtagtatga tccacggcta
     4621 tctttccttg cgttttttag gatactttac aaaagaatcg acaatcgatc cagaggacag
     4681 ctatttttgg atgatcaatg actttatcgc tactttgcct taaacaatca gattcattta
     4741 tatcattttc aagatgttca ttaaatgatc cttttataag agaaaattct tgcaactttc
     4801 ttgaaaagtg actaagataa gtctagaaaa gctgaaagaa ggagcacgag tatgtatcaa
     4861 gtaattacca tgttcgggga caatgagcct tggtggtttt ttgaagactg ggaagaagat
     4921 attgaagcag aagaaacatt cgagtctttc gaagaagcac gacaagcgta cgaaaaacaa
     4981 tggaacgaaa tccatcaaaa ttatgaatat atcaatgcaa aaagtaactt tctcagcgcc
     5041 ttttggaatg acggagatga gcgctggtgt gaagagtgtg acgatgattt acagcaatac
     5101 aaaggacttg cgttgttgaa aaatcatcag cccatcacag tagaaagcgg aaaggaattt
     5161 tatgaaacaa ctaattctag cggaaaaacc aagcgttgcg aaagacctaa gcaaggtgct
     5221 tggtgctaat caaaagcaca aaaattatta tgaaggaccc aaggtgatcg ttacttgggc
     5281 ccttggtcat ttattaggat tgaaaatgcc ggaagatctg aataaggaat ggcagagctg
     5341 gcaaatggac acgttgccaa tgatccctaa aaaactagga atcaaaccac ttcctaaaac
     5401 aggacatcaa ttaaaagcaa tcaagcagct ggctcagcga aaagatgtct cagaagcagt
     5461 cattgcgaca gatgccggaa gagaagggga acttgttgct cgctggatct tagagtatgt
     5521 gcatttcaat aaaccagtga agcggttatg gatctcttct caaacagata aagcaatcaa
     5581 aactggattc aagcaactaa gacctgcgaa agaatatgat gcattgtatg aatctgctct
     5641 tgcccgggca aaagcggact ggctggtagg attgaatgtg accagagcct taactgtgaa
     5701 gtacgcggat aatttatcag caggccgtgt gcagacaccg acactagcat tagtgagaga
     5761 ccaagaaaag aaaatcgagc agttccgtcc acaaacgtat ttcacgatca cgttgcaagc
     5821 tggaaaagaa caagccaaaa tgcttcaaaa aaatcaattt gctttaaaaa cacaagaaga
     5881 agcacaaaaa cttgttcaat tgatcagtac aagcaaagga actgtcacag aagtccaaga
     5941 aaaattaaaa acggaatctg cgccattgcc ttatgattta acagagatcc aaagagaagc
     6001 caatcaacgc tatggctatt ctgcaaagaa aacattaagt cttgtacaaa gtttgtatga
     6061 gacccataag atcgttactt atccgcgtac agacagcaaa tatctgacaa atgacatgaa
     6121 aagtacgatg aaagaacgat tgcaagcagt cagcgacttt gctcctgagg tcaagcagta
     6181 tttgaaaaac ggagccatcg tccaacagac aaaagtattc caagacagta aagtgaccga
     6241 tcaccatgca cttttgccaa ctgaaaacag agcacgttat gaaaaattaa gtaatgaaga
     6301 gcaaaaaatc tatcaaatga ttgtctctcg ttttttagga ctgtttgctc aaccccataa
     6361 agtcagccaa acaaaagtta cggttgaatt tggtaaagaa cgattcattt ttcggcaaaa
     6421 tcgtgtcgtc caagcaggat ggaaaggcga aactgaaact gagacggaaa cagtcaaatg
     6481 ggaaaaagaa atgagaatca atcccgattt caccatcaaa aaagaactat ctgcaccgcc
     6541 taaaccattg tcagaagcaa gcttgcttgg tcaaatggaa aaatacagtc tgggaacccc
     6601 agctacgcgt gcagagatta tcgagaaact aatcaaatct gaactgatgg aacgtacccc
     6661 tcaaggactg caagtatctc ctaaaggtaa gcaattatta gtattggtaa atccctcttt
     6721 agtaacacca gatttgacag aaagctggga aaaagacctt gaagctattg ctgcaggaaa
     6781 gaaacaagca ggaaccttct tgaatggaat cgaaaaagaa acaaaacgct tggtgaatga
     6841 gatcaaatca agcaaacaag aatatcaaga tttctcgatt acccaaaaga aatgcccaga
     6901 atgtggggcc aatctgcgcg agaaaaacac acgtgacggc aaaatctatg tctgcaccaa
     6961 ttcagattgc aactaccgca gaagaaaaga tccaaaagtc tctaatcatc gatgcccgca
     7021 atgtcaccgg aaaatggaaa tcatcgaagg taaaaacggt gcttatttcc gttgtaaata
     7081 cgatggaacg acagaaaaaa tgcttgataa aaaagagcag aagaagaaaa tgaccaaaca
     7141 tgaagaacga cgcttgctga aaaaatatgc acaagcagaa gaaccagaag aaagcccatt
     7201 agcagcagca ctaaaagcag caatgaagaa gtaataagat atgaaagagg ttgtga
//
LOCUS       DAKBVG010000132         7246 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000132 DAKBVG010000000
VERSION     DAKBVG010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.132"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            <1..212
                     /locus_tag="OUD09_001947"
     CDS             <1..212
                     /locus_tag="OUD09_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCT9693208.1"
                     /translation="IQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINEIEDEF
                     DVKIETEDGIKTVQDLVTYVEKQTA"
     gene            319..1236
                     /gene="fabD"
                     /locus_tag="OUD09_001948"
     CDS             319..1236
                     /gene="fabD"
                     /locus_tag="OUD09_001948"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCT9693209.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            1248..1985
                     /gene="fabG"
                     /locus_tag="OUD09_001949"
     CDS             1248..1985
                     /gene="fabG"
                     /locus_tag="OUD09_001949"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HCT9693210.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            2004..3239
                     /gene="fabF"
                     /locus_tag="OUD09_001950"
     CDS             2004..3239
                     /gene="fabF"
                     /locus_tag="OUD09_001950"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCT9693211.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIIGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            3242..3718
                     /gene="accB"
                     /locus_tag="OUD09_001951"
     CDS             3242..3718
                     /gene="accB"
                     /locus_tag="OUD09_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCT9693212.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPETMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            3743..4162
                     /gene="fabZ"
                     /locus_tag="OUD09_001952"
     CDS             3743..4162
                     /gene="fabZ"
                     /locus_tag="OUD09_001952"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCT9693213.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            4170..5549
                     /locus_tag="OUD09_001953"
     CDS             4170..5549
                     /locus_tag="OUD09_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCT9693214.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPTRHIEVQILGDHF
                     GNVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            5550..6419
                     /locus_tag="OUD09_001954"
     CDS             5550..6419
                     /locus_tag="OUD09_001954"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HCT9693215.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHML
                     LELHTQKGWIDRG"
     gene            6412..7197
                     /locus_tag="OUD09_001955"
     CDS             6412..7197
                     /locus_tag="OUD09_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HCT9693216.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRFDQHELLEKRYKRFRQY"
BASE COUNT         2435 a         1288 c         1715 g         1808 t
ORIGIN      
        1 aaattcaaga aatcatcgca gaagaattag gaaaagaaac tgaagaaatc aaattgacta
       61 ctgacatcca agaagactta gaagctgaca gcttggattt attccaaatc atcaatgaaa
      121 ttgaagatga atttgacgtg aaaatcgaaa cagaagacgg aatcaaaacg gttcaagatt
      181 tagtgactta cgtagaaaaa caaactgcat aatcattaaa attaggttgg gggctgggac
      241 atttcctgaa ataggaatgt ctcggccttt ttgaatagga agaatcaaac atatattctg
      301 gatatattgg agaaaaacat gaaaacagca ttcttattta gtggccaagg tgcacaatat
      361 caaggtatgg gaaaagattt atatgaagaa gccgttgtaa agcagacatt tgatgaagca
      421 agtgagattc tcggctacga tatggctgaa ctttgcttta cagaaaatga acgtttggat
      481 cagacgcaat atacacagcc agctattcta acagtcagca tcgcgtatta tcgcttattg
      541 aaggagcatg gaatcatccc agatgcagct ttaggactga gtttaggaga atattcggca
      601 ttagtagcta gtaatgcgct atccttcact gaagcagttg cgttagtagc aaaaagaggc
      661 gcttatatga cagaagctgc cccagctgga agcgggaaaa tggttgcagt gatgaatgcg
      721 ccgattgaaa cgatcgagga aagctgtcat gaagctagca agtacggcat cgtatctcct
      781 gcaaattata atacacctca gcagatcgtg attggtggcg aagaaaaagc agtagacgaa
      841 gctgttttac tattaaaaga aaaagggttc aaaagaatga tccctctaaa tgtcagcggg
      901 ccttttcata cagctatttt agaaccggca gcaaaaaaac tggcgaagga cctaagtcag
      961 attcaattct ctgaaccttc ttttcctatc ataagcaaca caacaacgga aataatgaag
     1021 aaagaaacaa tcgcgggact acttgaacag caagtcatgc aacctgttcg tttctatgag
     1081 agtatccata agttaaaaac aatcggcatc gaacaagtca tcgaagtcgg ccccggaaaa
     1141 gtattaagcg gatttatgaa aaaaatcgat aaaacaatac cagttctgcg tgttgagaat
     1201 aagcagacat ttgatgaaac aatagcaata ctataaggag gaacgttatg gatgtaaaag
     1261 gaaaaaatgt atttatcaca ggtagtacaa gaggaatcgg caaagcgatg gccttagctt
     1321 ttgcaaaagc tggagcaaat atcattttga atggtcgcgg agagattcca aaagaaaaga
     1381 tagaagaaat cgaagcattc ggtgtaaaat gcgtaggagt ttccggtgac atctctgatt
     1441 atgagaaagc gggacaaatg atcaaagaag cagaagaaaa gcttggatcg atccatgtgt
     1501 tagtaaacaa tgccggaatc acaaatgaca aacttgtgat gcgaatggat gcagaagatt
     1561 tcaaaaaatg tcttgatatc aatttgattg gaaccttcaa tatgacgcag cacgtattga
     1621 aaaaaatgat gaagcagcga gaaggggcca tcattaatct ctctagtgta tctgggttga
     1681 ttggaaatat tggtcaggca aattatgcag ctagtaaagc tggcgtagta ggattaacaa
     1741 aatctgtggc acgagaagct gcaactagag gcattacatg taatgccatt gcgccaggat
     1801 tcatcacaac tgatatgacg gaagttttag cagacaaagt caaggaacaa gcggaaaagc
     1861 aaattccgat gcagcgtttc ggacaagtag aagatattgc acaaacagca gttttcttag
     1921 cacaaaatcc atatatcacc ggacaagtca tcaatgtcga tggcggattg gtcatgcacg
     1981 gataagaaaa ggagagcaaa caaatgaatc gagtggtaat tactggctat ggagtcactt
     2041 cacctattgg aaatgacgca gacagctttt tgaatagctt aaaagagggg acgaatggca
     2101 tcgcaccgat cactaaattt gatgcttcag aaacagggat taccttagcg ggtgaagtca
     2161 aagatttccc ttacgataaa tattttatta aaaaagatag caaacgaatg gatatgtttt
     2221 cgatctatgg aatctacgct gctttagaag caatggagat gagcggttta gataaagaaa
     2281 aaatgaatca agaccggttt ggcgtcatca tcggatctgg gatcggtggt cttccaacaa
     2341 tcgaaaacca agttattcgt ctacatgaaa aaggtgcaaa acgagtttct ccaatgttcg
     2401 taccaatggc gattgccaac atggcagctg gaaatattgc tttgcgcgta ggtgcaaaag
     2461 gtatttgtac agctatcgta acagcttgtg ctagcggaac gaattcaatt ggggaagcat
     2521 tccgtaacat caaacatggc tattctgatg tcattcttgc tggaggaaca gaagcaacga
     2581 tttgtgagat ggggattgct ggatttgcag ctttaacagc actctcagaa tcaactgatc
     2641 ctaatcgtgg ttcgattccg tttgacgaaa atcgaaatgg ttttgtgatg ggtgaaggag
     2701 caggtgttct agttcttgaa tctttagaac atgcacaagc tcgcggtgcc aaaatctatg
     2761 gagaaatcat tggatacggt gcaaactgtg acgcttacca tatgacagct cctactccag
     2821 atggttctgg tgcgtctaaa gcaatgaagc ttgcgatgga agaagctggt gtacagccgg
     2881 aacaagtcgg atatatcaat gctcacggaa caagtacacc agccaatgac caggcagaag
     2941 caaaagcaat ccaaatcgca ttaggtgaaa atttcagaga tacttacgtc agcagcacga
     3001 aatcgatgac tggacattta ttgggagcag ccggcgggat cgaagccttg gcaactttgc
     3061 tggcattaga acatcaattt atcccaccta cgatcaatgt agaaaaacaa gatcctgaga
     3121 ttgacttgac agttgtcaaa aacgaaagca aacctgcaag ttttacttat gcaatgagta
     3181 attcattagg gtttggcgga cataatgcgg ttctgtgcat gaaacgctgg gaggaataat
     3241 tttggatatc aatgaaatca gagaattagt cagtcaattt gatcaatcct cgctaacgga
     3301 attcgatttg cgtgaaggtc aatttgaatt gtatatgaat aaaaataaaa caagtcgagg
     3361 catgaatatg gttcaaacaa acgaaacgtt gccagtttcg gagccagtca atgtcatacc
     3421 tgaacaagca acaactgctc cagaaacaat gaaagaaaaa gaatcggcag tatttgatgg
     3481 aatcgaggtt gtttcgccaa tcgtgggaat cgtttatctg caaccggcgc ccgacaaacc
     3541 agctttcaaa aaagtaggtg atcaagttgc caaaggagaa gttatctgca ttattgaagc
     3601 gatgaaactg atgaatgaga taactagtga ggccgacgga gtgatcacag aaatccttgt
     3661 agaaaacgag tcagttgtcg aatatggaca gccactcttt aggattcagc aaaaataagc
     3721 aaagcaggaa agagggaaat caatgaacat acaagaaata aaagaaatca ttccacatcg
     3781 ttatccaatg cttttgattg atcgagtgga agaaatggtt gaaggagaac ggatcgttgc
     3841 taagaaaaat gtaacgatca atgagccctt ttttcaagga cattttccag aagaacctgt
     3901 tatgccaggg gtattaattg ttgaagcgat ggctcaagca ggagcagttg cattactttc
     3961 tcttgaacaa ttcaagggaa agacggctta tttcggcgga ttggacaaag caaaattccg
     4021 taagaaagtt acaccgggag ataccttata ccttgaagtt gagatattga aagtgaaagc
     4081 ttcagccgga ataggcaaag gaatcgctaa agtagacggt aaaaaagtag ccgaagcgga
     4141 attaaccttt atgattggat aggtgaaata tgttttctaa agtattgata gctaaccgcg
     4201 gtgaaatcgc tgtacgtatc attcgtgcat gccgcgaact agggatccaa accgtagcga
     4261 tatattcaga agcagatgcc gaagcacttc atacacagtt agcagatgaa gcaatctgca
     4321 ttggcccggc aaaagccgtt gattcatatc tgaatgttca ggaagtactg agcgctgcaa
     4381 tcgtgacaaa agccgaagca attcatccag gatttggttt tttatctgaa aatagccgat
     4441 ttgcctccat gtgtgaagag tgcaatatca catttattgg tcctaaaagt gcaacgatcg
     4501 atgcaatggg aaataagatc aatgcgcgtc aattgatgca agaagcaaat gtcccagtca
     4561 ttccaggaag tactggtgta ttatctacag tagaagaagc attggaaata gcagacagaa
     4621 ttggttatcc agtgatgctg aaagcagctg caggtggtgg cgggaaaggc attcggaaag
     4681 ttcaatcaaa agaagagctc ccacagcatt tttcttcagc tcagcaagaa gcagcagcgg
     4741 catttggaaa tgatgatatg tatttggaaa aaatcattta tcctactcgc catatagaag
     4801 ttcagatttt aggtgatcat tttggcaatg tcattcattt aggagaacga gattgttctt
     4861 tgcaacgtaa taatcaaaaa gtactggaag aatctccttc tgtagtgatc tcacaaacaa
     4921 aaagagaagc actaggagac gctgctgttc gagcagctaa agcagtcaat tatgaaaatg
     4981 ccggaacgat cgagtttctg atggatgaag aaggcgattt ttatttcatg gaaatgaata
     5041 ctcgaatcca agtggaacat ccagtaacag aaatggtcac aggaatcgat ttagtaaaaa
     5101 aacaggtgga gatagcagcc ggtgagcctt tgaatgtgtg tcaagaagaa gtagtattcc
     5161 aaggacacgc aatcgaatgc cggattaatg cagaaaatcc tgctttccat tttgctcctt
     5221 ctccagggaa gatccaaaac ttactgcttc cagcaggtgg gatgggactg cgggtagaca
     5281 gtgcagttta cccgggatat acgatcccgc catattatga ttcaatgatt gcaaaaataa
     5341 tcgtccatgg aaatacgcgt tttgaagcat taatgaagat gcagcgagcc ttaagtgaat
     5401 ttatcacaga aggaatcatc acgaacgcag aatttcagat ggatttgatt agtcaccctg
     5461 cagtcatagc aggtgattac agcacagcat ttttacaaga agaattttta ccgaattgga
     5521 caccagaaac ggagatagga ggcgcataga tggctctgtt taagaaaaag aaatatattc
     5581 ggatcaatcc taatagagag agcaaacctg ctaactcgcc atctgttcct gataacatgt
     5641 gggcaaagtg tccaaattgc aaacatatcc tttatacgaa agacatcggt gaagaaaaag
     5701 tatgccctca ttgcggatac gcttttcgaa tcggtgcatg gcagcgtcta gctttgatta
     5761 ttgatgaaaa aagttttgaa gaatgggata cggatcttgt aaccaaagat ccgctagcat
     5821 ttcccgaata tacggaaaaa attaaaaaaa tgcaagataa aacaggactt catgaagcag
     5881 tcctcactgg taaagcaacc attcaaggga ttcccttcat tattggagtg atggatccta
     5941 attttatcat gggaagtatg ggaacgattg tcggagaaaa gatcaccaga ttattcgaac
     6001 gagcaacaaa ggaaagtctt ccagtcgtct tgttcacagc ttcaggtggc gcacggatgc
     6061 aagaaggaat cttttctcta atgcagatgg caaaaatctc tgctgctgtc aaacggcata
     6121 gcaatgaagg tcttttttat ctaactgttt taacggaccc gacgaccgga ggagtaactg
     6181 ccagttttgc aatggaaggc gatatcattc tagcagaacc ccaaagcttg atcggttttg
     6241 ctggaagacg cgtcatcgaa cagacgatca agcaggaact tccagaagat ttccaaaagg
     6301 ctgaattctt attatctcat ggttttgtcg atcagattgt tccccgcatg gaattgaagc
     6361 agaaaatcca tatgcttctt gaattgcata cacagaaagg atggatagat cgtggataag
     6421 acagcacatg atgtcgttca gttagcaaga gctaaagaac gatggacaag cttggatttt
     6481 tttgaagaga tcttcgattc tttcgaggaa tgccatggtg atcgtttgtt tgcagatgac
     6541 gaagcgattg ttggtggaat cgctcgatta gatggcaaac cggtaacagt gatcggtatt
     6601 caaaaaggac gggatctgca ggaaaatatc cgcagaaatt ttggttctcc ccatcctgaa
     6661 ggctatcgaa aagctttacg actgatgaaa caagcggaaa agttcaatag accggtgatt
     6721 acattgatca atacagcagg tgcatattgc ggtgtaggag cagaagagcg cggcgaagga
     6781 gaggctattg ccagaaatct gctcgaaatg tctgatttaa aggtaccgat catcgccatc
     6841 atcattggtg aaggtggaag tggcggtgcc cttgctctag gtttagcgga tgaagtctgg
     6901 atgctggaag aaacgattta tgctgtgctt tctccagaag gatttgcgtc gattttgtgg
     6961 aaagacggta gtcgcgccgc agaagcagct gagttgatga aaattactgc gccagaacta
     7021 cgcgagttag gaatcgttga tcgagttatt cctgaaacgt tcaacggcga gcagttgcca
     7081 cgatataaag tcgcacacat tatgaaaaaa gcgttgattg aaagattgac ggaacttgtt
     7141 cgattcgacc agcatgaatt actggaaaaa cgatacaaga ggttccgcca atattaggtg
     7201 tttaacaact aagcaaagag taaagaatgg tcttgctcgc ttagtt
//
LOCUS       DAKBVG010000133         7214 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000133 DAKBVG010000000
VERSION     DAKBVG010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7214
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.133"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(<1..2045)
                     /locus_tag="OUD09_001956"
     CDS             complement(<1..2045)
                     /locus_tag="OUD09_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HCT9693217.1"
                     /translation="MENKIHCTTGRVITLVLFLFLLLFARGTAVQALEKVDPSQIKEN
                     NNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAHYQKV
                     GTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQSINV
                     MNAKLEFKYAGTKTKVALSEPQPEIQSKMRSMRAAGIDLNDTFLSIYSLNGQYQQRVS
                     WYNDNNESVGERNIDMREFVGYEKMGSLPYFVTTDTACAEYKAPALSTNNLTSKVVGG
                     RAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTALLWTP
                     QPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKSFKIT
                     DTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSFLANA
                     ANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNTVENYFSLKPNKKVE
                     QDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIASDHYKQFSIQDPVED
                     RLTYKEGSARVIDMSTGKDITSEGTLTYDSNSRTLKWEASADFLSKNLLDGREIQLIF
                     TAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQIIVPKTGSNHLVLTVIG"
     gene            complement(2346..3170)
                     /locus_tag="OUD09_001957"
     CDS             complement(2346..3170)
                     /locus_tag="OUD09_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693218.1"
                     /translation="MNKRKWLFVCVAALFSTVAYSQLSFADSSDSSTTQTIDNSITAP
                     SETDSSENTTEETADSNISFTESTNPTSESSSETSEKPQDVEDTQVIKQAIHIQKIIS
                     SNQINNQGSQVAQQEGINGAKFIVYDITNILEEMSQDDNEKNQSVEIIESKLKDRAKK
                     LSYDQLKKVAEGETKTIDDQEGVIEFSIEVPANKKQAYYIVNESSPENISNSEDIVLL
                     TPVSNEKGEFLTDVWLYPKSEKSEPKEEIKKVVSTGVKKNFFENCWEFFAQLFFDN"
     gene            complement(3167..3922)
                     /locus_tag="OUD09_001958"
     CDS             complement(3167..3922)
                     /locus_tag="OUD09_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCT9693219.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPVVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSISLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKN
                     RKSISLDWRLALLFAFFCVPIIFIYKKIRRRSE"
     gene            complement(3935..5911)
                     /locus_tag="OUD09_001959"
     CDS             complement(3935..5911)
                     /locus_tag="OUD09_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HCT9693220.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGGLSAVVYFKKKA"
     gene            complement(5964..6635)
                     /locus_tag="OUD09_001960"
     CDS             complement(5964..6635)
                     /locus_tag="OUD09_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HCT9693221.1"
                     /translation="MKKWLFGFLGVTLIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPGYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            complement(6695..6904)
                     /locus_tag="OUD09_001961"
     CDS             complement(6695..6904)
                     /locus_tag="OUD09_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693222.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVAQYINDMQLT
                     GKEKACLLGSMKNFEQLRRTYRIHF"
     gene            complement(6971..7210)
                     /locus_tag="OUD09_001962"
     CDS             complement(6971..7210)
                     /locus_tag="OUD09_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693223.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKALSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
BASE COUNT         1977 a         1485 c         1089 g         2663 t
ORIGIN      
        1 cttccaataa cagttagcac taaatgattc gatccagttt ttggcacaat gatctgaggt
       61 aagttaggtt ttaccccaat cgttactaca ttagttgctt tctcaatact ttctactgaa
      121 acttttgctt ggttgtcgat atccaattct tcattcaaag gagttttagc tgtaaatatc
      181 agctgaattt ctcgtccatc taaaagattt ttacttaaga aatcagcgga agcttcccac
      241 ttcagcgttc ttgaattgct atcataggtt agtgtacctt ctgaagtaat atcttttcct
      301 gtactcatat ctatcactcg cgcagaacct tctttatacg tcaaacgatc ttccactgga
      361 tcttgaatac taaattgctt gtaatgatct gaggcaatgt ctacaccttt tgtatgccat
      421 ttttgtgtga ctagatattg tacttcagat cctgcattta aaggagctgt aggcgttcct
      481 tttttaccat catttcgacc atcaacatct ttcccatctt gttccacttt tttatttggt
      541 ttaagagaga aatagttttc tactgtattt gttttttgtg ttcctttgtt cggatcattg
      601 ttaaacgtta cagtagccac atttggtgca attccttttg catcattttt tgctctcata
      661 tggagctgca aaatataatc cctaccgtaa tttgcagcgt tagctaaaaa gctgtcagaa
      721 gcagtataag tgactgtatt acctgataca gttaactttc ctcctgtatc agcttttttc
      781 caagtaccat tcgcttcaac ataccacaat gcgccatcat agtttttccc atccgatacc
      841 atatcaaaat ttttgctgtc aaaggtatca gtgattttaa atgatttgta tttgttcatt
      901 gtgttgactg ttaatgcatc aaacttttgg tgaatatgat agtaaacatc tgatccttgt
      961 ggaatcactt ttttgtcaat attgtgtgct gcctcttcag tattagcttt gttaaacact
     1021 tcttttgtag gcttcgatgg ttgtggtgtc catagcaatg cagtatcaaa gctaaacatc
     1081 atactattcg tatttgcgcc aatcatgaat gttggggaag cgccttgaag cgtatacgtt
     1141 acaccacttc tgtgataagt atcagctcct acaacatcgg tgaaatgatc attcgagcta
     1201 taagcctttt ctgcacgtcc tcccactact tttgaagtta aattgtttgt tgataacgca
     1261 ggagctttgt attctgcaca tgctgtatct gttgtgacaa aataaggtaa gctacccatt
     1321 ttttcatacc caacaaattc tctcatatca atattacgtt caccgacaga ttcattattg
     1381 tcattatacc aagacacacg ttgctgatac tgtccattta aactgtaaat ggatagaaat
     1441 gtatcgttta aatcaatacc agctgctctc atcgatctca tttttgattg aatttctggc
     1501 tgtggttcac tcaaagctac ttttgttttt gttcctgcat acttaaattc tagttttgca
     1561 ttcatgacat tgattgactg catgaaaaat cctcgataaa tatttttatc aaagagcaaa
     1621 taattataat actttttgcc attaattgtt ttagctaatc cgtcttggtt aaccccagcg
     1681 tgcgctacaa aattagaaaa agtcacgtca caatcaattt cttttcctag ataagtacct
     1741 actttttgat aatgggcaac gatttttgac gtacttttca aatctttgac ttttggccaa
     1801 gcaagtactt tttgtttttt agttgaatac tcatctagta tcttaaatcc attgatccca
     1861 gttacttcga tatttctcaa tgaatcacta tcatgataaa gcaccagatt attattttct
     1921 ttgatttgac tcggatcaac tttttctaag gcctggactg cagttcccct cgcaaaaaga
     1981 agaaggaaca aaaacaacac taaagtaatt actcttcccg tagtgcaatg aattttattt
     2041 tccataatgc cctccatcta atattttttt aaacgatcgt aagcttttca ttcttttttt
     2101 cctaataccc acgttaccaa tcctgtaaga agaaccaaac taaccataaa aattggattg
     2161 atcaagagct ttttaatcac ctctaatggc atggaagcac attgaatgtt tagaaaatga
     2221 atgaacaatc gttgatatct tggataaatt gataattgag aaaaaattaa accgcaacac
     2281 aatattgttg cggctaaaaa ggatagatta aaaactaata ttcttattat tttcatcatt
     2341 tcatcctaat tatcaaaaaa taattgagca aaaaattccc aacaattttc aaaaaagttc
     2401 ttctttacac cagttgacac aactttttta atttcttctt tcggctctga tttctctgat
     2461 ttagggtata accaaacatc cgtcaaaaat tcaccttttt catttgaaac aggtgtcaat
     2521 aaaacaatat cttcactgtt tgaaatgttt tcagggcttg attcattaac gatatagtat
     2581 gcttgttttt tgtttgcagg tacttcaatg gaaaattcaa tcacgccttc ctgatcatca
     2641 atcgttttgg tttctccttc agccactttt ttcaactggt cgtatgaaag tttttttgct
     2701 cgatctttca attttgactc aattatttct acagattgat ttttctcatt atcatcttgt
     2761 gacatttctt ctaagatatt cgtgatatcg tagacaataa acttcgcgcc attgatacct
     2821 tcttgctggg ctacttgact gccttgattg ttgatttgat tcgaagaaat aattttttga
     2881 atatgaatgg cttgcttgat cacttgcgta tcttctacgt cttgcggctt ttctgaagtc
     2941 tctgaagaac tttctgacgt tggattcgtt gattcagtaa agcttatatt tgaatctgcc
     3001 gtttcttctg tagtattctc cgaactgtcc gtttcgcttg gtgcagtaat tgaattatct
     3061 attgtttgag tggtactact atcactgctg tcagcgaaag acagttgcga ataagctaca
     3121 gtagaaaaga gagctgctac acaaacaaac agccattttc tcttattcat tccgatctcc
     3181 tccttatttt tttataaata aaaataatag gaacgcaaaa aaaagcgaat agcagtgcta
     3241 atcgccaatc caatgaaatt gatttcctat tttttgcttt tttctgtagt tttaaatctt
     3301 ctttactgaa ggaaactcgt tcaccataca ctaaaaagcg gtgtgagttg acaccttttg
     3361 gtgtgcatgt cattaacgtg atcctatctt ggtctttttc aggcataaga taatctaaat
     3421 aatttttttg taattcttct gctttaactt cttcaattcg ataaatttga taggcatgtt
     3481 gttcttcatt taccttaata aagaattttt gccctttttt tactgaaggc aaatcatcga
     3541 aaagattatc tttataaagt ccactgtgcc ccgcgattaa aggattttta ccgtttcctc
     3601 ctgtaatatc gccagttcct tccgtaatcc ctacaccatt ctctaaaatt ttgtcggaag
     3661 ttccgtcata aattggtagt tttaaagaaa tacttggaat tgtaagaaca ccaacaggat
     3721 cgcctaactc tattttcgca acatccaaag aagaatctgt tttttcaaga ttttcaatgg
     3781 ctaaaattct ttgctgttgt tgaaatttcg ctacatttgt ttccacaact ggaattaaat
     3841 agactgctac tgccgacagc aaccaaaaaa atatcatcag tttgtgcatg actcttcttt
     3901 ttgggggctt acgaataatc attagctgat ccttctaggc tttcttctta aagtaaacaa
     3961 ctgcagaaag tccccctgct cctagcaaca agcaagagat caatacgtaa gattggaaac
     4021 cgttgctacc agtcattggt acggaagctt tttgattatt tttgatcgtg attcggtttt
     4081 cttctttgta tgaatctttg tctactttga aagagatctc ttttgttaat ttttgatagt
     4141 tttcaggagc ttttgtttct tccagtgagt aatctccttc agttaaacca gtgatttcaa
     4201 aatgaccctc tttatctgat tttaggattg ctgcatttgc tacatcgtct acccaaactg
     4261 cttctttgac accatcttta tctgtatcag taagtccacc gtagtactta ccttctgcat
     4321 tcttagctgc aaattctgcg ccttcaagcg gtgttgcttt gatacctgtt ccaaataggc
     4381 cgctatcgac ttttaagaat tttgcgccat aagtcacaac gtcagctttc gttttttcgt
     4441 ggtgttcttc ctgattaaat ggattattgt tataaacgaa atccacgcta tttgttaatg
     4501 gatttgcgat ttcagcacta tcattcacaa acatgttata agtcattttt agatcttgac
     4561 catgtaaatt cgcaactgta tcacttactt gtccattatt tacgataaaa tcgatcacaa
     4621 aaccactatc agaattttcg attttatagt cagtattttc ttttaatacg actgaaccat
     4681 cattatttgt tactttaata tctgcttttt cagcaacata agaaagtcct ttacctgctg
     4741 tatctcccca tttaaataca ctgtaaggag aagtttcgct tttcacaccg tttactgtga
     4801 taccgtctgc gccacctgca atgtctgttg gaatatgaat cgttccttga taatttactg
     4861 cgtcaccaat ttggaaagaa tgttcttgtc ctgttccatt tgttactgtt ttatcgatca
     4921 ctggtgcttt atagtttacg tatgaagctt ttaagtcact tgtcacgcca tcattcgtaa
     4981 tttcaaaggt taatttattg tgcgcatcgt tgcgagcgtc tgcgcctaat aaatattgat
     5041 ctgctgttgg ttcttgatct gatcccacta caacttccga aggaacttcc acaaaatagt
     5101 attttcctaa cgtcaaatca gtcgcagtca gctttccttg atcatccgat acaagatcac
     5161 ccaattttgt tccttttcca ggttcgccct tgtataactc aaattttgcc ccttttaaaa
     5221 gggtttgttt gctttggtct gcgccatcgt caccaaattt cgttaattca aaactcaact
     5281 tagaaacaat gttcttagga tacaaatgaa tattttttaa aaatcctgat cctgttttat
     5341 ctgtcattgg cgcaatcaca accattggtt ttgctttttg tgtgactaat ccagcagcgg
     5401 atttactttc ataaaccaca tatacattcc ctttggaatt gacataggct ggttgatcag
     5461 taaaagtgat ctcaccattt tcatctacag ctgaagtaat tttgttggtt gcattcttga
     5521 cgtactcact gttagatccc ttgtcttcta catcttgtac aatctcgtcc actttcgcgt
     5581 tggttagatc agaatcttct ctcggtaata cttgatccgt aatatttcct aacgtaaact
     5641 caacatcacc ataatctgcc ttgttgtatt tcgttacacc tgtaggatat tgatcgtgcg
     5701 tataaccatc gtttttgatc ccgtcaatat caacattcaa ttgcgttcct tgatcgtaca
     5761 ttaatttatg aaccgtgata ttgaccttct ctggtggtgt tttttgcgtg acatcttctg
     5821 catgtacttg atacccctga aagcagcctg caatgattcc taacattcca acaagcattc
     5881 cagctttttt tacataccga tttattgcca ttttttactc tcctcatttc atatttcaac
     5941 gactcgcgtt gattaaaacc ttgttattgt agctctccct gtaccacgta tctttctttt
     6001 ccttgtgacg tataaacaca agtaaacaac gttaataagg gtttctctcc tttctttggc
     6061 aaagacaaga aagagttctc ttcaggaacg ccatcgatta atttaaagta cccaggtacg
     6121 ataaatttac gcgttacctt gtaacgatag acgccatcgt tactaactaa gtccatcacc
     6181 tcccctaatg acaacttttg aatatcagaa aataaaacac ctggcatttc catgttatga
     6241 cctgctagga catagtttcc tttgcccatt tcggcatctt cataaaaata agtagccgct
     6301 ccaagactca aagtgtattg atttgcccct ttaaaaattg gcaatgctaa accaagtgca
     6361 ggaatctgaa tttgtgcaaa tacttgttta ttgacgccca tttcgattgc ttctttttgt
     6421 atggacaaca tttgccccaa agacatatca ttattgacgt tcgttgcatc ctccttataa
     6481 ctctttgcat gtgcattgat ttgatctacg ggcatctttt tttctatatt ttgcttaaac
     6541 acctctcctt catgtttttg gtagctaaca tagccaaaca cactgcaaaa gacgattaaa
     6601 gtgactccta aaaaaccaaa taaccatttt ttcatccatc tgcctccttt cagacaaaac
     6661 aaaaaaactt tttctcattt ccatagaaaa agttttaaaa atgaattcta taagttcgtc
     6721 tcaactgttc aaagtttttc attgaaccta acaaacaagc tttctctttt cctgttaact
     6781 gcatatcatt gatatattgc gcgacagata atcttttaag gtgtttttgc ttcatcgttt
     6841 ttagtaactt ttcttccatt tctttcgaca ctttttttct gttttcgatt tcttcataat
     6901 tcatgagttt tcctaccttc cttgtttttt gcaaaaataa aaaagcctat gatcacatac
     6961 gctctacact tcagtcttgg gttatctttt tctttcgttt aattactggc cgtgctggtt
     7021 tcaaatcctt gattccatta tgggttctcc aataatcgaa ttcttttcgc aatctttctt
     7081 tttccatatc tgttccttta gataaggctt tgaatacttc tttaggtgaa taaccacgag
     7141 ctaacagctg tacatacctt ccttttgtca aagtatccat atccatcgcc tctaaaactg
     7201 gtcgcgccat ctac
//
LOCUS       DAKBVG010000134         7120 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000134 DAKBVG010000000
VERSION     DAKBVG010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7120
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.134"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            34..711
                     /locus_tag="OUD09_001963"
     CDS             34..711
                     /locus_tag="OUD09_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HCT9693224.1"
                     /translation="MGLAFLLAGCTNGNESKKESTKETAKATIATTTTTSTESKPTAA
                     STETADTSTSSESSKTTETTQTSTSSSSVEASSSESSEQVEQTLWNADKSAQLEAFMA
                     TWGQTMGQQYKSYTNQMSVDLYGLKVPQVILNGEWKMAIGGVPVSAEWSESGTGQADY
                     QITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFNETANNELKNG
                     FNQIVYG"
     gene            1032..2273
                     /gene="sstT"
                     /locus_tag="OUD09_001964"
     CDS             1032..2273
                     /gene="sstT"
                     /locus_tag="OUD09_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HCT9693225.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEPT
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            2420..3055
                     /locus_tag="OUD09_001965"
     CDS             2420..3055
                     /locus_tag="OUD09_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HCT9693226.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            3246..3989
                     /gene="truA"
                     /locus_tag="OUD09_001966"
     CDS             3246..3989
                     /gene="truA"
                     /locus_tag="OUD09_001966"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCT9693227.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYRS"
     gene            4223..5056
                     /gene="msrA"
                     /locus_tag="OUD09_001967"
     CDS             4223..5056
                     /gene="msrA"
                     /locus_tag="OUD09_001967"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCT9693228.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDTRENISVKEQ
                     LSKLEVTLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQDERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            5170..6130
                     /locus_tag="OUD09_001968"
                     /pseudo
     CDS             5170..6130
                     /locus_tag="OUD09_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
     gene            6419..7117
                     /locus_tag="OUD09_001969"
     CDS             6419..7117
                     /locus_tag="OUD09_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCT9693229.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNIA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNVLAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
BASE COUNT         2333 a         1163 c         1483 g         2141 t
ORIGIN      
        1 gttttttaat ttaaaaaaag gattttagta acaattggtc tagctttttt gttagctggt
       61 tgtaccaacg ggaatgaatc gaaaaaagaa tcaacaaaag aaacagcgaa agcaacaatt
      121 gcaactacta caacaactag cacagagagc aagccaactg cagcaagtac ggaaacagca
      181 gatacctcta cttcgtcaga gtcttccaaa acgactgaaa caactcaaac atctacttca
      241 agttcttctg tagaggcttc atcttcagaa agcagcgaac aagtcgaaca aacactgtgg
      301 aatgcagaca aatccgcaca attagaggca tttatggcta catggggcca aacaatggga
      361 caacaataca aaagctatac gaatcaaatg agtgtggact tatatggtct taaagtccct
      421 caagttattt tgaacgggga gtggaagatg gctattggtg gagtgcctgt ttctgcagaa
      481 tggtcagaaa gtgggacagg tcaagcagac tatcaaatca cagccgtcta ttccgatgca
      541 gagacagaac catatctgaa aaagcacgtc tatctattcg ggtttcagca aaatcagcca
      601 aaagtattgg tgacacaaca aaatcagggg aatcccgata attatcttta ctttaacgag
      661 acagcgaata atgaattgaa aaatggcttt aatcaaatcg tttatgggta agtaaaccag
      721 tgagagtgag agaaagaaga gactcagtgg aaaatattaa ttgattgcta tgtctaagaa
      781 ttaagatgaa ggatgaaatt tctagtttcg tccttcatct tagttacgtt ttacttggat
      841 aactataata ataacgataa atagttttct ctcgacttct ttagagattg aataaaccct
      901 ccaatactaa aattgaatgt tgcaagagca tgaattgcca attgtattat tagaattttt
      961 agaaaattgc acctttagtt tctttagata aaatgataca atgattgtta aaattgtgaa
     1021 agggtggtca tttgttagag aagtttttgg atatgtcgtt agttcaacgt attgttgttg
     1081 gtattttagt cggaatattg tttggtatct ttttccccac gtggacattt atttcaatat
     1141 tggggacgtt gtttgtcagt tgcttgaagg caatcgcacc tattctggtt tttttcttga
     1201 cgatggcgtc aattgccaag cataaaattg ggaataagac attcgtgaag cctattttga
     1261 tcttatattt agtgggaaca ttgctttctg cacttgtagc agtcattgta agttcaattt
     1321 ttactgttcc tattacttta caagaaacag tatctgaaaa agcaccgcaa agtttggaaa
     1381 gtgttttatc tactatgttg acgaatgtca cacaaaatcc tgttcaatct cttattgatg
     1441 cgaattacct tggcgtttta ttctgggctg tattgttggg actagctttt cgagcacgtt
     1501 cagaaccgac aaaagagttg gtggatcaga tatctatagc gttgtcacaa gtcgtacaag
     1561 tgattattgc atttgctccg atagggattt taggattggt ttatcaatca attgctacga
     1621 ctggaatcgc tggattggca gagtatctgc agcttttact tgttttgatc ggtacgatgc
     1681 tgtttgtcgc attagttgtg tacccattcc tcacgttttt gtttattaaa gaaaatccct
     1741 atccattgat tttcttttgt ttgaaagaaa gcgcacttcc cgctttcttt acccgaagtt
     1801 ctgccgcaaa tatcccgata aacatgatgt tggctgaaag attgaaattg acaaaagagt
     1861 cttattccat atcgatcccg ttaggtgcta cgatcaatat gggcggtgcc gcgattacca
     1921 tttccttgat gacgttgact gctgtacatt ctttagggat tgaagcacca tttgttttga
     1981 aagtcctttt atgtattttg tctgctttag ctgcttgtgg agcttcaggt gttgcgggcg
     2041 gttcactgtt attgattcca ttagcatgta gtttgtttgg tatctctaat gatgtcgcga
     2101 tgcaaatcgt tggtattgga tttattatcg gtgttatcca ggattcaatg gaaactgcct
     2161 taaattcgtc aagtgatctg ttgtttactg caataggaga gctaggcgcg cgtaaaagaa
     2221 gtggggaaaa aatcaaattg aaagattgtt tagatggatt aagtgaacgt tgaacgttag
     2281 atatttagta tttttaaaat gattcgagtt tttaatgagt tatatagatg ttcgtgtaaa
     2341 tagaggtttc tgttaaatca agataaagtc gtttataata acaattaaaa gcataggtaa
     2401 ttgttattgg aggcgtatca tggatttttt acgtaaattt ggttttttct ttttattatt
     2461 aggcgtgctg agtgattttt tgactccgta cgtattagga ttgtattatc ctgaattaga
     2521 tcaaatgaag agcgtgatca gtgtatttgg agaggtttcc agtcctgtaa gaaaagcgtt
     2581 tcttatttgg tctgtggttt cagggatctt tttcatcctg tcattgcctg cattgtatgc
     2641 tgcatttgta agtacatctc gtgtgttagc taccctagtt acagcagcca tcgcgttcta
     2701 tggtataggt gattgtatct ttacaggttt gttcagtatc aatacccatg aatcttcgtg
     2761 gaatgtctcg acatgggtcc ataatatcgg atcaggttta ggttacagtg gttttttatt
     2821 gtttccagtt cttcttttct tgatctatcg aaaaaatggg caaatggacg taagccactt
     2881 ataccttgga ctgtctgtga tcagtttgct ttttgcggcg atttatggac tggcaagaat
     2941 tccttctata aattcttggc cattactgaa taaaataggt ttttgccaaa gaatcagttt
     3001 tttctttaat tatgtgccca ttgcctggtt gagtatcttg catctcaaac agtaaaactt
     3061 gagccgtcaa attgaacagt cagcggatac ttcatccggt ggctgttttt tgtttttctg
     3121 aaatatatat ttccacagtt ttcttgtata gcttctgtct tagagaagca aggagctata
     3181 ttttgtgcta caatcttaaa aaggtagaga gaagaccaaa tggaagacag aggagccact
     3241 aaaatatgag aaacatcaag ttgacaatcg aatatgacgg aaaaagatat tcaggatggc
     3301 aaagacttgg agatgacgat aaaaccattc aaggaaaaat cgaaaaagta ttgcatcaga
     3361 tgacaaatga agagatagaa attattggat caggaagaac agatgctggc acccatgcgc
     3421 gaggacaagt agcaaatttt aaaacaaaca cagaaattgg actggcagaa atgatcgact
     3481 ttatgaatcg ttaccttccg cgggatattg tcatcaaaag aatcgaagaa atgcctgagc
     3541 gcttccattc aagatataat gcggtgggca aaaaatacag ctattatgtt tggaacaacg
     3601 tgattccttc agcatttgag cgaaaccaca gcttttattt ccctcaagag ttggatatgg
     3661 acaagctgaa tgcagcttgc gaaaaattag tgggtacaca tgatttcatt ggtttttctg
     3721 cattgaaaaa atcaaaaaaa tccacagtgc gtacgattga tgaattatct atcgaacgaa
     3781 agggagaaat gcttcatttt acttttgtcg gagacggatt cttatataaa atggtgcgga
     3841 ttatcatggg gacattacta gaaatcggca caggtgccat gccgatagaa acaatcgatg
     3901 aaatatttga aagcaaagtg cggcaacatg caggagaaac cgtccctgca caaggtctgt
     3961 ttttggatga agtttattat cgctcataaa aaagaatcat ctttactcga cttctgagaa
     4021 ttggagaaca tccctttttt cagaggtcgt tttttagtta aaccaaacca tagagtatgc
     4081 tttttaagat aaagatcatc ttatatcatc tgaagctgct tatcttcttt gaataaatac
     4141 gattcaatcg attatttcct cactttatga taaattacag ctaaagtcac tagcttgtct
     4201 tcttcggaaa ggaggagaat ctatggatga ggaacaaata ttcattcaaa cactttataa
     4261 tctgattttg aatccgaata cacgagattg ggaacgaaaa gtattgattc agacaaagaa
     4321 tgacacaaga gaaaatatat ccgttaaaga acagttatca aaactagaag taacactacg
     4381 tccattagct atccgaatga atctgacgcc tgatgtaatg gacttttatt tattgattac
     4441 ggaaggattt gataaggagc aaaaatatga cttttccaag catgccatgc aagatgctga
     4501 ctatcaagaa cgtgcagtat ttgcaggggg atgtttctgg tgtatggtcg agccgtttga
     4561 atcgaaaaaa gggatcctat ctgttttgtc aggatacacg ggcggacatg ttgaaaaacc
     4621 gaattatgat caagtaagtg gtggttacac aggtcatgtg gaagctgtgg agatcattta
     4681 tgacacaaga gaaataagtt atagcgagtt gctgacaatc tattggcaaa ttactgatcc
     4741 cacagataca tttggacaat ttcaagatag agggaagcaa tatcgtccag tgatttttta
     4801 tcaagacgaa cgacaaaaag aattagcgga acaatcaaaa cagaaactag acagttcagg
     4861 aactttccac caaccaatcg tcacaaaaat cgaaccagcc ggtacgtttt ggccagcaga
     4921 aaactatcat cagcaatttt acaaaaaaca accgaaacgc tataaaaaaa tccagcaagc
     4981 acgtaatcaa tttctgatct atcaacgagt aaaaaacaaa tggcaaaaaa atatacgaaa
     5041 aaatcatttt gattaactcc ttttctttaa aatgtagtaa actaaaaata gaaaacaaga
     5101 aagcgtttta aaaagatttg atgaggagga gaaaaattgt atattgcaga tagtgcacgt
     5161 tatgaaaaaa tggaatatcg taaatccggt cattcaggtt taaaattacc gattttatct
     5221 ctcctctcgg attatggcaa aattttggtg attatgatcc tatccataat caaagagaga
     5281 ttttaagagg cgcattcgac atgggaatta cccatttcga ccttgcgaat aattatggag
     5341 gccctgctgg agctgcagag aaaaactttg ggcgtatttt tagagaagat tttcaagcct
     5401 accgtgatga actgattatt tctagcaaag caggttatca tatgtgggaa gggccatatg
     5461 gggaatgggg ctctcgtaaa tccatcatat caagttgtga ccaaagtttc caaagaatgg
     5521 ggctagatta tgttgacatc ttctatcatc atcgcccaga ccctgataca cctttggaag
     5581 aaacagcaga agcgttgatg caacttgtaa gacaaggaaa agcattatat attggtatat
     5641 ccaactataa tggagaagat acaaaaaaat gactgaaatt ttaaaaagaa aggaagcacc
     5701 gttcattatt catcagatgc gctacaatat gttttctcgt gcactactag aagacgatct
     5761 atctccagta ttagaagaag aagggctagg ggcaatcacg ttcagtccgc ttgctcaagg
     5821 gttgctgaca aatcgttatc tacatggtat tcctgaagat tctagagcac atcgtaagga
     5881 aataccattc ctttcagaag aacaagttgg atcaactctg gaaaaaatca aagcactgca
     5941 aacaatcgcc gtatcacgag gacaatcatt agcgcaaata gcccttgcat ggaatttacg
     6001 acaaaaatct gttacaagtg tactgatcgg agcaagtcgg ttaagtcagt tacaagaaag
     6061 tgttcgaatg atggataatt tagatttctc accagaagaa gaattgagaa tcgaccaaat
     6121 tctagaatag aaacgatgta atcaaacttt ttatccatat attaaaaaaa acaaaagatg
     6181 acatcaagaa tcgaatgaag atcgtctgtt attgaacatc atattttttg tcgatcaaaa
     6241 taaaaatgat cgaaggctag gagtgaattt tatcctagtc ttttttatgg taaatatctt
     6301 cttcgtttgt atgtctatga acggattaat cgatttttta tacaaaaaat catcagatga
     6361 ttcaaacgtg ttataataat tacgaaaaaa tacatcagat gaaaaggagg tatgtggttt
     6421 ggaagaaaat aaaaccctag tagaagtaac tactgacaag atccttagat atttaaagga
     6481 aatgcagttg caagtaggag ataaactgcc gaatgaatat gaattgtccg attatttaga
     6541 ggtggggcga agcacggtgc gagaagctgt gcgggctcta gcttccagga atatccttga
     6601 agttcggcaa ggagcgggga catttgtcag tgataagcga gggattgcca atgacccact
     6661 cggtttttct ttgattgaag atcaggatcg gatgatacga gacttatttg aactgagata
     6721 tttattggag cctaagatgg caggtttggc agcggaatat gcaacgaaga atcaaatcgt
     6781 ccaaatgaaa aaattaacgc aaaagattga aacttctttt cgagaaggca cacaagaaca
     6841 tgttcaatta gacatcgatt ttcatacgat gattgcagaa gctagcggaa acatagcctt
     6901 gctgcatatt atcccaatca ttaatgaatc gattgctttg tttaataaaa attatgatat
     6961 ccagcatctg aaaatcgaga cgatcgatat tcatacagag atcgttcagg ccatcgaaag
     7021 gcggaacgta cttgctgcgt ttgacgcaat gactgtccat atggcaataa atcggagaga
     7081 attacagaaa ataataggag aaaaagaaac taattgacag
//
LOCUS       DAKBVG010000135         7089 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000135 DAKBVG010000000
VERSION     DAKBVG010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7089
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.135"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(170..3319)
                     /locus_tag="OUD09_001970"
     CDS             complement(170..3319)
                     /locus_tag="OUD09_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HCT9693230.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMESVKKESYLDPTVFEAEEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKESDWLIKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTAWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVQSSTLNNQIQLLEE
                     QIGVYETVKGSLMEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDNLIHA
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(3333..4550)
                     /locus_tag="OUD09_001971"
     CDS             complement(3333..4550)
                     /locus_tag="OUD09_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCT9693231.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKMFV"
     gene            complement(4540..6135)
                     /locus_tag="OUD09_001972"
     CDS             complement(4540..6135)
                     /locus_tag="OUD09_001972"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HCT9693232.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYSISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHEQ"
     gene            6260..7045
                     /locus_tag="OUD09_001973"
     CDS             6260..7045
                     /locus_tag="OUD09_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HCT9693233.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKSTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
BASE COUNT         2049 a         1447 c         1179 g         2414 t
ORIGIN      
        1 tgcaacagaa ccggtaaatg aatgaaaaag atggaagaat cattattcaa tagctttctt
       61 aaatactccg ttataaaatg tgaacaagta aaaagacatc tatccctatt ttctacactt
      121 ccactttact actatctttg aatcgtacaa ttttcttaac tcttctcgtt tacctcaatt
      181 cattttcaag tggaatcaac tcatcatcta acattttacg ccatgctcgc ttcatttctt
      241 gtgggtattt aaatggtttc gcttctggat gtttggcttt atatccttcg taatccttac
      301 tgttgataat tgctttcata ttaggaattg gatcttcacc tggcatatat tgcagattag
      361 aagcatgtaa ttctttctca gaaacaaacc actcattgga aaaggcatga atcaaattat
      421 cacttgcttg tgtaaaatag cgacgtttaa tgacaaagat atcttcatta ctatctaaag
      481 tccccacatc aaatgcattt aaaatttgtc ggtaaacttc tttgactcct tctgttttat
      541 tcaatttagc caaagctttt tcaatttctt ctcgtgcatc tgaactattc gcagcataag
      601 ttcctagtaa ttgatcaata taagtagaat caatatcata caacttagtg gtattttcac
      661 tataaaattc gatcgttgaa aaatcttctc cctctttttc tggttcttcc tccatcaatg
      721 aacccttcac ggtctcgtac acgccaattt gttcttccaa aagttgaatt tgattgttca
      781 aagttgaaga ttgcaccatg tcatcattgt aatcatcata agtcactaac gattcatacg
      841 cacgattcaa ttcttgaaat gctttgacgt actcgatacg gatttcaagt ggcgtatgtt
      901 catcaatgga ttccatggat gaaacctctt ttaacttacc atatgcttgc ttgaatcttt
      961 ttttggattc ttggtaactt ggataaatca aacccgattc ttctttagct tctgaataca
     1021 gttttgtcgc atcctcgaca ttcttctcca ttgtcgctgg ctttcggaaa gtaacaatta
     1081 gtccttttgt tttttcagga tacgtacggt tcgtacgtga aaaggcttgg atcaatcctg
     1141 catactctag attacgatcc acaaataggg tttgaatcgt aggggcgtca aaacctgtca
     1201 atagacgatc cactacgatt actaaatcga tttgtttccc aaattgtttg aattccgctc
     1261 tttttcgtgc caaacgatta ttgatgtctc cgttataacg ctcaatgtct gctagtgacc
     1321 aggcagtatc ataatactga ttatattcct caataatttt ttgcatttct tcttgttggg
     1381 cacttgaatc tcggctattt tcttctaatg aataggtaat agcgattcgt gggaaatcag
     1441 gatcctccat cgttcgtcct tcacggatag gctgattggg aaactctttt attaaccaat
     1501 cagattcttt cgtcatctct ttgatggccc gataatactt tttggccata tcaatggaac
     1561 tcgtggttaa gatcgcagac ttcgttggac ggccatttcg aaaatcaaat tttgtatagg
     1621 cattgtctgg acggaacatt ttatgaatga ctttttggat atgttcctct gcttcaaaaa
     1681 cagtagggtc caaataactt tcttttttga cagactccat ttgatcgatc atacgattga
     1741 tctgttccgg tgaatactct gcatatgttt ctacttcttt taatttccga tagattttat
     1801 tctctaatga agtcggctcc acggtgtttt catgctctac ttgaaatcct aaaaccgagc
     1861 catcttctaa tgcattcttg atggtatagg tgtgcaagac ctctccatac tgatcatggg
     1921 tcgtacgtgc caattgccct ttggcttgtt tccgattctc agggaaaatc ggtgtacctg
     1981 taaagccaaa ccaagtggat ttcggaaaca tttttttgat ttcttccata ttttcagcac
     2041 ttaacgctcg atgacactca tcaactacaa acacgatatg ttgccccatt aatttttgga
     2101 agcgattggt tccttttttc tcttcctgtt tctttgcata tttcaacgcc gcatctagtt
     2161 tttgacgggt tgtaatgatc acaacattcg cattggcatc tgccaacaga gtttcactta
     2221 actctttggc acttcctgtt cccacgatca agctattcgc tttggcatta ccagaagaaa
     2281 ccccagtatt aaactccgaa gcaaatttcg taaactctgt ggtggtctga ttatctaaat
     2341 ctttgcggtc cactaacatg atcgttcgat cgatccccga ttttcgtgct aaaagcttag
     2401 tagatacaaa actagtcaac gtctttcctg aaccagtcgc atgccacaca taccctgatt
     2461 gatgtttatt tgcggcaata aacaaggctt gaatcgcatg cacttgataa ggatgtaaca
     2521 ccatgagggt tttattgtct tgatcttcac tgacaatcgt ataatccgca atcaagcgat
     2581 gggcatctgg gatatttagc acttgtttgc aaaactcata taagttttct acctttttat
     2641 tatctttcgt tcgccaacta aataaaaact taggatgcaa atccttcggt aaggcatttg
     2701 caaaataccg agtcgtttgt tcattggaaa ccacaaacaa ctgtaaggtc gaaaagatat
     2761 tgttccgaaa catgccttct tcagcatatt ttttgatttg attaaacgcc tgataaaaac
     2821 catcttttgc agttacttgt tttagctcga tttggataat cggtaagcca ttgatcaata
     2881 aggtgacgtc aaaacgtcga tcccgcgctt ctatgttact tccacgtttt gcaatttgat
     2941 ggaccacttc ataagtagag attcctccac caatgtcttg attggaataa aggataagtg
     3001 agacagaacc caattgcgga ttttcccgtt caatcgtgat ccgagccatc ccattttcac
     3061 cttttaacca tttcgcagca tcaaatgggg ttttcgtccg tagcaataat tcggttttga
     3121 tcgtttcaaa ctctttatcg gtcaaaggag aatcattcag ttctgcttga ttatttgaga
     3181 tgattttttg acgtaaattg ctccaaagat cttcttctga ttttagatcc ggacgatacg
     3241 tccactgatt atgtccttcc ccgaggacac gaatcaactg ttcttcgact tccgcttcct
     3301 cacggtgact aattttcatt cttcttccct ccctaaacaa acatcttttg caagaagcct
     3361 tttttcgttt ccttcagcaa atctaactta cgttgatgaa gagcgatcgt atcatctagc
     3421 tgcttgaaga aggcaccaat tttttgttgt tcatttatat ccggtggaaa ctgaaatgtc
     3481 ctttgttcta taccaccttt agtcacatgt accattgtcg ttccgttagt tgattcttta
     3541 attttctctt tatcagtcaa tagccaagta tatagatact gtttgtccaa ttccttagat
     3601 aaaaatttaa gtttccaaat atgataatgg tagattacac gttcttcatt ccacatttca
     3661 ggaccgaagc ttgttgccca taaatataag agatctcctt tattagcata cttgtcttcc
     3721 tctaattcta agtttgaata ataccatcta tcattagtat taaaattacc tactcttaaa
     3781 accctatatt ttccagaatc aagtaattct tgttgtttaa aagctctacc attcaaaaaa
     3841 actaccactt cccccaactt ccgctcttcc caatctcccg taaatcctgg aaaacgaact
     3901 tctggcactt ttgctccgtt tttagggaac attttttgta agaatccttt tttcgtttcc
     3961 ttcagcaaat ctaattcgtg ctggtgaagc atgatggtgt gatcaagttg tttgaaaaat
     4021 gaaccgatct tgatctgttc gtcataagtt ggaagtgaga taggtgtctt tttaccacta
     4081 tcaattgtat aggttccgac ggttccaatg cctgcaacgt cacggatata cttctgaatt
     4141 ttgttattta tgaacgagta gtaaaagaag tttccgtcaa ttttatgctc attcttgaac
     4201 caaatgatgt ttccatcctt aaagtaaatt gggttatcat cagtgattaa taaggggaca
     4261 cctattgatc cgacaccagt tacaagcaag tctccttgag aaactttacc tgaatttttc
     4321 gaatattcat tatatttttc ttctgaaatg taaagataat cggatggttc ctcatttttt
     4381 gcagctgaga caatatcacg agcacgtaaa aatctaatac ctgagttagt ccaatctgat
     4441 tgatgaattc gcttcacaga agtaacatcc atcatatctc ctaacttacg ctcttcccaa
     4501 tcttccgtaa aaccaggaaa ccggatttct ggtcgtgtat cattgctcat gtttgaacac
     4561 ctccagcgca ccttgtaacc acgcttcatt ttcaggtgtt gtttgcaggg aagagatagc
     4621 ttctaaaagt tctttttcta gcacttgctg ttcttgtcgt atcttcttga tgtcatttcc
     4681 gacatgaacc atatcaattg gttcttcttc ttcgaaagta tcgacatagc gtgggatatt
     4741 taagttatag tcattttctt tgatttcatc aaaggttgcc aaatgagcgt atttctcgac
     4801 atcttttctt tcagcgtagg tttcgaggat tttttgaatg ttttcttccg ataatttatt
     4861 ctgatttttc cctttaacaa attcacgact agcatcaatg aataatacat ctcgagtttg
     4921 acgattcttc ttcaatacaa tcaccgtcgt tggaatcgat gtaccaaaga acaagttcgc
     4981 aggcataccg atcacagcat aaatactgcc gtcttctaat aatttttgac gaatgacccc
     5041 ttctgcagca ccacggaata aaaccccgtg tggcaagacg atagccatag tccctgtttc
     5101 cttcaaatga tagaaaccat gaagaagaaa cgcaaaatct gctttggatt ttggcgctaa
     5161 tttcccgtag cgattaaacc gagaatcatc taagaaggtc gtatctgctg accaattagc
     5221 cgaataaggt ggattcatca ctactgcatc aaatgtgtaa ggttcatctg ttggccagtc
     5281 cttattcaac gtatccccat tgcggagatt catttcttcg gcatctaccc catgtaaaat
     5341 caagttcatt tttgctagat tgtatgtcgt tgtatttagt tcttgcccat gatatttaac
     5401 gttatctgga tgagttaagt agttacgtac atttaacatc aaggaacctg aacccatggt
     5461 tgggtcaaag acactaaaga atcgtcgttc tttttgatcc aacgtcacga tttgtgccat
     5521 catatcagaa accatatgag gcgtgtaaaa ttctccggct ttcttccctg cttctgaagc
     5581 aaattgactg atcagaaatt catacgcatc cccgatcaca tccccatcgt gacctaaaac
     5641 ttccacgtca ttcaatttct tgataacttc ggtaatcgtc acatttcgtt gttgctcatc
     5701 tgttcctagt ttttttgatt ggagatccac atcatcaaat aacccattaa attgattgta
     5761 ggtagatgcc aattgcacaa aggctttgtt tagatcgttc aattggaacg ttgcttgttt
     5821 tgcttgatcc gctaagacgt tgaaaagata ctctggtgag atcgaatacc ccaaaatatc
     5881 aacgattgtg gcaatcaggt cttcttttga ttcttcatct gaaagtaatt cacgatacaa
     5941 cattgtttgt ttgcttactg tgtcatattc ctctaaggat tcatctgcta aaagaaccac
     6001 ttgttccaac aaacggtcgg ataaatattt atagaaaatc agtcccaata aataattttt
     6061 atattctgac gcatccattt tactacggag attatctgcc gcactaaata atttttgatt
     6121 taattctgca cccaaaaaga tacactccta tctcttattt aaagttgcta tataccctct
     6181 tattttagca tactcccttt tagaaaaagc aaaaaaaaaa gaaaaaatct tattcttgat
     6241 aacaagctac gtgacgtata tgcctaacaa aaatagaact tttgaatcaa tcttaaatga
     6301 aggtttaacc acagaaccgg catccattat ttttgatgaa gttatcgatg acgttgaaga
     6361 tagtaagtat cataaaaatt taaaagaact tttttatttt gcactcaaca atggaccaag
     6421 tttgttcact ggtatatcag tacctgatag tgataatcca ttaattatgg caaaaattta
     6481 cttaacaaaa tggtgtaata aatacatcag ggataggaat aacccagcat taaaaaaacc
     6541 tcttaaaaca tttggcgaaa aagatgctgc tttaactact agagtagcag caaatgctaa
     6601 tattgatgat caaaaagtat tgaatgacta cttgcgtgga cattttttat atatgagcgc
     6661 tgagaacatg aacggaagta ttttagagga atatttagcc gaagtgttag aacctgaagg
     6721 ttggatttgg tgcgccggct ctgtatatcg agctgttgat ttctgttatt taggaacctc
     6781 accaattctt ttacaagtga aaaataaata caatacagaa agcagttcga gttcagctat
     6841 aagagtagga acaacaatta gaaaatggaa tcgtttaaat aagtcgacaa aaatttctgg
     6901 tttggatagc cctatcccca attggaaagc attaatagaa atgactgaag ctagtaagga
     6961 actggcagct aagttaactg aaaatagtta tttagcttat attaatgaaa aatccacacg
     7021 agaattatgg acactcgatg actaacaata tccttattag tatctatcca cgatacatgg
     7081 aaaaaatta
//
LOCUS       DAKBVG010000136         6857 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000136 DAKBVG010000000
VERSION     DAKBVG010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.136"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(199..612)
                     /locus_tag="OUD09_001974"
     CDS             complement(199..612)
                     /locus_tag="OUD09_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HCT9693234.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1068..1553)
                     /locus_tag="OUD09_001975"
     CDS             complement(1068..1553)
                     /locus_tag="OUD09_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693235.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(1692..1907)
                     /locus_tag="OUD09_001976"
     CDS             complement(1692..1907)
                     /locus_tag="OUD09_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HCT9693236.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2131..2931
                     /locus_tag="OUD09_001977"
     CDS             2131..2931
                     /locus_tag="OUD09_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCT9693237.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            2950..4818
                     /locus_tag="OUD09_001978"
     CDS             2950..4818
                     /locus_tag="OUD09_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HCT9693238.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            4811..5302
                     /locus_tag="OUD09_001979"
     CDS             4811..5302
                     /locus_tag="OUD09_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HCT9693239.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            5290..5844
                     /gene="srlA"
                     /locus_tag="OUD09_001980"
     CDS             5290..5844
                     /gene="srlA"
                     /locus_tag="OUD09_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HCT9693240.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            5862..6857
                     /locus_tag="OUD09_001981"
     CDS             5862..6857
                     /locus_tag="OUD09_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCT9693241.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2397 a         1097 c         1330 g         2033 t
ORIGIN      
        1 attatgataa atggtatcca aaacaaaagt ttacttaaga caatcaaatg atcaactccc
       61 atatttttta ccaagagaga tcataacatc tttatcgtaa aaacaaaata tttttttgct
      121 cattttgaaa atttaaacat actcgttctt atccaaatat ataaaaatcg cccctcattc
      181 atcgagaggc gataagttct aatttaactc aatttttttt gtttcttttg gctgataagc
      241 tgttttaggt aataccaatt tcagcacacc gtttttaaag gttccagtga ttttttcttc
      301 atcaacatta ggaagataga atgaacgttt gtaagagacc gaatgtcttt ctttccgtac
      361 atagttgcca tcttctttat cttctgtatg ggactcatga ttggcggaga tcgtcaaagt
      421 attatctgaa tattcaactg tcgtgtcttc tttgtcacag ccaggcatat cagcagttag
      481 tttgtattct ttttcatttt cgactaaatc tacttttgga taacttgaaa cacctaaaaa
      541 atcattgaat gctggactaa acacgtcatt gaaatcaggg aacatgtcgc gaatgctagg
      601 tacatttgcc atttttgatc atcctttcaa taagtatttt ttatttatcc ctatttacgt
      661 aataactcta ttttgaaaaa aatgcaagta taatggttag ctttgtgaat atttcatcta
      721 atacgataca aaggctatct ttctaatttg aaaaagggat cattaaaata ataagaagaa
      781 aatatgtcaa aaggtaaaaa agtaaattct tctatccaaa tatagaaaaa ttgaatctag
      841 aaaaccgccc gacaataaat ttcattaact cagttgaccg aaaatgaagt agataccaaa
      901 aatttatcaa gagagcaagt agagaagtga gtatattcat aagagaaata gctgtaacga
      961 attattaggt taatgcaaat gcttatttat actccgtata actttctcag aaaaaatgat
     1021 accaataatg acttgctagt actgtaaatc cgtataatat catctaatca aaatggtgtt
     1081 ccatttgcat ctgtcatcgt aatcgatcct tcgggtgaca ctcgataaaa accaacgctt
     1141 ccagatcctc cttgggaacg gatttgtttg ctggaagctg tgaacaaata atctcgatca
     1201 attttttgat aaaagctaat aacaatatcg tcactttgta attcggaacg gtgggcaata
     1261 agaaatagaa gtgctttgtc agagttttct aatggaagag aaggtacagt gctttcttgc
     1321 atttcagatg ttgaagaaat gctaaacgac tgaatacttg attggctatg ttgcgaatct
     1381 acattaccag tagaagtatt agtaatggaa gtagtagaag tacttttagt tgaaccaacc
     1441 tcagttgttg aaatggctgc tgaagattct ttaacagaat tctttgtttt atatccacag
     1501 gcagaaaaaa gtattacagt tcccaacaag cctgcgtaaa tccctttatg catgatagct
     1561 cctcctaaaa tagattatct ttattcagta tatcacttaa acaaagaaaa gcagatggtt
     1621 taaaatgaaa aaaccttccc aataaaggga aggttcatga agataataac ttttttaata
     1681 attatagctt tctaatatct ttcatcaact tttctagtaa tcattgggaa aaatttcaag
     1741 cagtagatcg cacaaatacc tacgacgata taaatgatct tcgcaaaaat agttgttgaa
     1801 ccaccagcaa tcatagctac taaatcaagt tcgaacaagc ctaccaacag ccagttcaac
     1861 cctcccacaa tcaataaagt taatgcaata gcatctaaag ttttcattag aattctcctt
     1921 tcttaaatga taaaacaatt atatatcaaa aagagaaaca ttgcttatat aacgctcagg
     1981 aattttagca atctatgcaa aaagatgaaa attttttttg ctcttccatt agtacacatt
     2041 ttttagctga agctgtaaat ttcatgtctt aatttttttt agtgagcttt tatactggat
     2101 ttgtaagaaa aaagaaggag aggatcacat atgtcggatt ggttggatat tgcaggaaaa
     2161 gtagtaattg taactggagg ttcctcagga attggaagaa gtatcgtcga aaatttgttg
     2221 aagcagaatg cacaagtagc gaattttgat gtaactgaat gccgtataca gcatgaaaac
     2281 ctactatctt taaaagtcga tgtgtcttct aaaacggaca tagaagaagg gatatataaa
     2341 gtaatgaaac atttcaagac gatcgatggt ctagtcaata atgcaggaat aaatattcct
     2401 tctcttctaa tagatagaaa tcatccaaaa agcaagtatg aattatcaga acaagttttt
     2461 gataagatga tcgctgtcaa tcaaaaaagt gtatatttga tgagccaagc agttggaaga
     2521 atcttggtgc aaaagggatc aggagtgatc gtgaatcttt cttctgaatc aggattggaa
     2581 ggatcagaag gacagagctg ttatgctgct acaaaagcag cgatgaacag ttttactcgt
     2641 tcttgggcaa aagagttagg aaagaggaat gttcgtgtgg taggtgttgc accagggata
     2701 ctggaagaaa ccggtttgag aacacaagaa tatgaagaag ctctttctta tacgagaggt
     2761 ataagcgtcg aacagctgag aaacggttac agtcgaacaa gtacgatccc tctaggaagg
     2821 agtggtaagc tgcaagaagt ggcggatcta gtctgctatt atctttcaga aagatcaagt
     2881 tatatcactg gagtcacgac taatatttca ggaggtaaaa caagaggata aaggataggg
     2941 gggacgcacg tgacgctagt aaacagatgg taccaattat tgaaattact agtcactcac
     3001 aagaaagttt caatccagga agtgcaaaaa gaaatcaaaa ccagtaatca aaccataaaa
     3061 aaagatattg gggagttgaa tgaagaaatt gccggtattg caaaaatcat ccaaaaaaac
     3121 aaacattttg aacttgaaat ttatgatttt gatttttttg atgagatcat gcaaggaaaa
     3181 ttgaaaacag ccagtgattt caattcggca tccaaaagaa tcgcttattt actaaatagg
     3241 ctgatcgaaa cagaggattt cttacgtatg gatgatttgt ctgaagaagt cggtgtcagc
     3301 agaggaacag tggtcaagga tctaaagaca ttaaaagaga tgatcaatga ttttgatgta
     3361 aagattacag gcacaccaaa taaaggacta agaataatag gagatgagct agaattacga
     3421 ctacttttat tttattttgt ttctccctac ttttcagaga cgaaaacaga acagtttcta
     3481 gaagaaaaac aagcaatcaa acattttcag aagaagacga atgctagtaa acaagaaatc
     3541 acccttttaa cgaaagttat cggtatttct ttgaatagaa tctctagtaa ctatctgttg
     3601 aagaagccca tcaatcgtta cagtgggtgt tttgaatata tccaagaatt caatgaactg
     3661 atcaatcagt tggaagaaat ctatgaacta actttgagcc aatatgaaaa agattttatc
     3721 agttttcctt tgaacatcat caacacaagt ataacgatga taaggaaagc taactctgat
     3781 ttgcgcttat atttcgaccc catgatgatg cgtattcgac gattagatct cattgatctg
     3841 gatgagtcct tcttatatca agagatgaaa cagcacctct ctttattgat aaatcgggta
     3901 atttttcgtt atcgattgac tgatttattt tatggtgaga tagaaaaaaa gtatcctttt
     3961 tcttaccaaa tcgctagcga atgcatacaa gcattagaag ctatcttgca acataagatc
     4021 tctaaagtag agatcagcta cctaacgttg tatttggagt tagcatttcg atatcaaaaa
     4081 gagcattttt cctctaaaaa agtagccatt gttcatacga caggaaaagg aacagcatta
     4141 atgatccaaa gacaaatcaa aaggatattg gggagggata tacaaataac acatgtttca
     4201 gaagaacaat atgaaaaagt agatctaaac aaatatttcg ctgtttttac aacgattccg
     4261 ttaaaaaatg taaatgaaga gacaccggtg atccatttga ctaatctttt tgatgactct
     4321 tggttaaggg cacaatggca aaaagcaaaa aaaaagcgat tagccgggtt tcattctact
     4381 gtttttttct atcaaatatg gaaagaaaaa aagacgtatc aagaatattt aaacaaatta
     4441 gtcgctcctt tgatcagagg ctattatgtg gatgaagaat ttgaaaaaag gatcttgaaa
     4501 agagaatatc agcaaagcac atttgttaac aaagaatttg cttttcctca cacaaccaac
     4561 cagctatctc aacacattgt tttatcagta gggctaataa ctgatcaagg tttagcagaa
     4621 acaggaggaa atggagttat ttttttattt gccatacctg aagaaatgac ggctgaagtt
     4681 gagaatgaat tatttgagct atacgatatc ctatttgaac tcataaaaat gggaatagat
     4741 gagtttattg aattagcacc gacacaagaa tcatttttaa aactaatcaa aagtaaagga
     4801 gagagacttc atgaatgata tgtatctatt catagttttc gcagcttcag cttatgtgct
     4861 gcaagcagtt atcagttgga agcaactaaa gcatttcaat caaacatata gcgaactgcg
     4921 aagaaaagga aaagtagcga ttggtaaacg acctgggaaa atccgttcag gcacgattgt
     4981 tatgctagct atagacgatc atgatcaagt tttagaagtc agaaaaatgc aaggggttac
     5041 atctttggca aaatttcatt cattacctgt ctatgcgggt gaagatatcc attacatcga
     5101 tcgttaccat ccctcagttc gagcggaaga tcgtttgaca caagcagctt tagaaaatgc
     5161 cagagaagta tacttacgaa tagaggcagg aaatgaaaaa agtgaacaac cttcattgcc
     5221 ttttattgct atgctcaata attttcggat atggaaaagt acgatcaaag aaaaaaggag
     5281 tgacaaacga tggatatcat aacaaaattt gctgagggat ttatgaggct gttccaaaca
     5341 ggtgcagaaa cattcataag ctggatgaca ggaatcgtac cagttgtctt gatgctgatg
     5401 gttgcaatga atacgctgat tgctttttta ggtgaagacc gagtaacgaa agtagccaaa
     5461 ttatcagcga aaaatccgct tactcgttac cttgtgttac cgttcttatc tgcttttatg
     5521 ttgggaaatc ctatgtcatt tacaatggct cgtttccttc cagaatacta caaaccaagc
     5581 tattatgctg ctcaagctca attttgtcat acgagcaatg gtgtattccc ccatattaat
     5641 cctggagaat tgtttgtatg gatggggatt gctcaaggga tcgaaacctt agggctaaat
     5701 tcgatggagc tggctatccg atacttgctt gtgggactgc tgatgaactt tattggtggt
     5761 tggataactg attttactac tggatttgta tgcagacagc aaggaatcgt tcttagcaaa
     5821 aaagtagagc tttcagttga ttagagaaag gagagtttta aatgtatggt gatattaaga
     5881 tcgtaagagg aaacggcggg tatggaggtc ctttgattat taggccgaca aaagataaac
     5941 acaagttcat ctatgtcaca ggtggaggtg agaggccaat gatcgtagat aaaatagaag
     6001 aactgacagg tatggagtct atcaatgggt tcaaaacatc aatacctgat agtgaaatcg
     6061 cgcttgcaat cgttgactgt ggagggactc ttagatgtgg aatttatcct aaaaaaggga
     6121 taccaaccat caatatcgtt tccacaggaa aaagtggccc attagcacaa tatatcactg
     6181 aagatattta tgtttcagca gtagggttag aacaaataca gccactaatg gaaactgatg
     6241 ataacaaaga aacagtaaat actgcatctc cagataaaaa gtacaaatat gatacttcta
     6301 aaaagataac cgagcaaaaa gcagagcagc aaaactggat agcaaaaatt ggaatgggcg
     6361 cgggaaaagt agttgctaca ttcaatcaat cagcaagaga agcagtagat acgatgatcc
     6421 ggacagtgat cccttttatg gctttcgttt cccttttgat tggtgtgata caaggttctg
     6481 ggatcggtaa tgtatttgct aacattatgg cacctttagc aggaaatgca gttggattaa
     6541 caatcattgg ctttatttgt tcactgccat ttttatctcc tttgcttggt ccaggggcag
     6601 ttattagcca agtgattgga acattgatcg gtgtagagat tgggaaagga aatattcctc
     6661 cccaattagc attaccggca ctatttgcta tcaatactca aaatgcatgt gattttattc
     6721 cagtcggtct aggtttggaa gaagcaaaag ctgagacagt agaagtaggt gtaccatcag
     6781 ttctttattc aagatttctt aacggcatcc cacgagttat cattgcttgg gtagcaagtt
     6841 ttggattata tagctga
//
LOCUS       DAKBVG010000137         6847 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000137 DAKBVG010000000
VERSION     DAKBVG010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6847
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.137"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            60..1214
                     /locus_tag="OUD09_001982"
     CDS             60..1214
                     /locus_tag="OUD09_001982"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HCT9693242.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            complement(1236..1988)
                     /locus_tag="OUD09_001983"
     CDS             complement(1236..1988)
                     /locus_tag="OUD09_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCT9693243.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(2352..5018)
                     /locus_tag="OUD09_001984"
     CDS             complement(2352..5018)
                     /locus_tag="OUD09_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HCT9693244.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNE
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5360..6811
                     /gene="cls"
                     /locus_tag="OUD09_001985"
     CDS             5360..6811
                     /gene="cls"
                     /locus_tag="OUD09_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCT9693245.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQKALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
BASE COUNT         2021 a         1407 c         1246 g         2173 t
ORIGIN      
        1 aatcttttta tgatacctta gtaaagtaag ttttttatca taagtaaaga ggtgttgaaa
       61 tgaaaatagg gattgatcgt ctttcctttt ttattcctaa tttatattta gatatgacag
      121 agttggcaga aagccgtggg gatgatcctg caaaatacca tatagggatt ggtcaagacc
      181 aaatggcagt caatcgtgca aatgaagaca tcattacact aggagcaaat gctgccagca
      241 aaatcgtaac agaaaaagac cgtgagctaa tcgacatggt catcgtcgga acagaatccg
      301 ggattgatca ttcaaaagcg agtgcggtaa ttatccacca tttactgaaa atccaatctt
      361 ttgctcgttc ttttgaagta aaagaggctt gctacggtgg caccgcagct ttgcacatgg
      421 cgaaagaata tgtcaaaaat catccagaac gaaaagtact agtcatagca agtgatattg
      481 ctcgttacgg cttggcaagc ggtggtgaag tgacgcaagg tgtcggtgct gttgcgatga
      541 tgatcactca aaacccgcgt attttatcga ttgaagacga cagcgtattt ctgacagaag
      601 atatctatga tttctggcgt ccagattata gcgaatttcc tgttgttgat ggtcctttat
      661 ctaattctac gtatatcgaa tcattccaaa aagtttggaa tcgacataaa gaattgtcgg
      721 gtcgaggact cgaagattat caggcgattg ctttccatat tccgtatact aagatgggaa
      781 aaaaggcatt gcaaagcgta ttagaccaaa cagacgaaga aaatcaggaa cgtcttatgg
      841 ctcgctatga agaaagcatc cgttacagcc gacgaatcgg taatctttac actggttcat
      901 tatacctggg gctaacttct ctactggaaa attcgaaatc actacagcca ggagatcgca
      961 tcggtctatt cagctacggt tctggtgcag taagtgaatt ttttacaggt tatctggaag
     1021 aaaactatca agaatatctc ttcgcacaat ctcatcaaga gatgctagat tctcgaacac
     1081 ggatcactgt tgacgaatat gaaacgattt tcagtgagac gcttcctgaa cacggagaat
     1141 gtgcagaata tacttcagat gtaccttttt cgattacaaa gatcgaaaat gatattcgtt
     1201 actacaaaat ataataaaat cataaaaaga acccttcacg agtcgtgaaa ggttcttttt
     1261 ttatctgcgg accaaaggag cagtctatag gtcagcttag tcgtactaag gatttctcgt
     1321 ttttcactga atagtttgcg aatgtacctg gtcacaacag gatcttttgc gtacgcgtca
     1381 tagtatagct tcatcaattc ttcagtaatt gattcctgtg taaaataagt aaaatctttt
     1441 ttcttcactt tgaatccttc atcaattaag tatgtttcaa attgatccat tacaactggc
     1501 gatgtattag gatcttcttc tatcattcct agttgctttt cgataggata aaaaacatca
     1561 tcgattgatt ccactaccct gattatcacg caccatttgc ttgacttttt catcaatttt
     1621 tctaattgaa taagatgtac caatgctgga tttgctgctg taaaaaccat ttcatatgtc
     1681 tcatttgatt ggataaattc ttctaacgac tgtttgataa aggaaacatt tggcaactca
     1741 tttttttctg cttctttttt tgcattttct agcattcctg aagaaaaatc gatcaaatca
     1801 tagcgattaa tttcttttgc taaggaaaga aatctgccag atcctccagc aacgtctgct
     1861 gctgtttcag caggcaggac tttctccgtt ttcagataat tttttacatc ttcagcaata
     1921 gttacttgcg attcttgctg gatttcataa tattcttcag caaattcatc ccattctttt
     1981 tcattcattt gatttcctct aattttttgt tatatgtttt cacttttcgg aataaattat
     2041 tctcctcttt tatctattct atctcttttg atgaataata aaatgattgg ctttccgtct
     2101 atcataaata aaaaaaggga ctgtgaccaa agtcgtgtca aagtccctct ttccgaataa
     2161 acagtgtcca atcagatgac cctcagtatt agctcaaaat gctgatttta caacttcttc
     2221 cttaaacggt gttctcctgc caactcttcg gtcacaagtc acaatattca acaaacatga
     2281 gaagctgttt gttgaaattg caccttgtga ctcagaatta aacggcaggc tcacaacctc
     2341 gttgattcgt tttatttatt tccttgtact cgtacaatga tcaatttagt gatttccatc
     2401 aaaacaattg tcacaagtgc gattcccata catgtaaaga cttctgtcaa tccaaatgct
     2461 gatgggatag agaagacttc tttcagtcct gggatcattg ttaagcagta aagcgcgcta
     2521 catacaacta cagctagtaa gacacttctg ttagagaaga accctgcttg gatagctgtt
     2581 tgtgtgtttg aacgtgctgg gaatgtttgc aatgtccgac accaaatcaa tgttgaaaat
     2641 gacatggcta caccgaattc aggtgaatgc tgcatgccaa gccattgagc aaagataaca
     2701 gcaatcccaa tcagcacacc acgatacgtt acagaaatca atgttttgtc cgtaaagatt
     2761 cctgaatttg gatctcttgg cgggcggttc atcgtagcag gttcagcttt ttctgtacct
     2821 aatgcaattg ctggcagtga atcattgact aaattgatga acaacagttg taatgctgta
     2881 aatggattgg accagtcagc aactaaggca aagatgatcg caatgatcgc acctaaattt
     2941 cctgcaaata gataagaaat agctttcttg atattatcaa acacattacg tcctacttct
     3001 actgcgctga caatagaagc aaagttatca tccgtcaata ccatcgctgc tgcatctttt
     3061 gccacatctg tacccgttcc catagcaatt ccgatgtctg cttgtttcaa tgctggtgcg
     3121 tcatttacac catcacctgt catagctgag atatgacctt tattttgcca agcacgaacg
     3181 atacgaattt tattctcagg tgaaacacgt gcataaacag tgactttttc taaaacttcg
     3241 tttagttgat cttctgtcaa ttcatctagt tccgtcccgg tcaaagccag gtttccttct
     3301 tcaaaaatcc cgatttgttt tgcgatagca acagctgttg ttttgtggtc accagtaatc
     3361 atgattgttt tgattcctgc acttcttgct tctgttaccg cctgagtcac ttcagcacgt
     3421 ggaggatcga tcatagcaag caaaccaaca aggatcaaat cgtcttcatc ttcaaatgat
     3481 aaatcaaaat tatcaagtgg acggtaagca aaagctagta cacgtaatgc gcgtgttgaa
     3541 aaagcttcat tttgctcttg gaattttttc atcaattcat ccgtcatcgg aacgacttca
     3601 ccatttagca atactttttt actgcgttgg aagacgatat ctggtccgcc tttgacaaac
     3661 aagtaatttt cgttatcgat cgtgtgtcca gtagacatta attttcgatc agaatcgaaa
     3721 ggaagttctg cttgtcttgg gtagttgttt cggatttcct gataaggctg gtttaatttt
     3781 tcactaaagt cgataaacgc tacttccgta gggtcgccca atttagtacc gtcttcactg
     3841 atcgatgcat cattcgctaa aacactgatt tcgatcaagc gacgttcgtc agctgtccat
     3901 tttgaagggt catttgtgaa atccccagta tcgccattac ctaaaaagta gtcaacaact
     3961 gtcattttgt tttgtgtcaa tgtaccagtc ttatctgtac agatgatact tgtcgaacca
     4021 agtgtttcaa ctgctggaag cttccgaata atggcatgac gttttgccat tttattcgtt
     4081 cccaaagata acacgattgt tacgatagac tgtaatgctt ctggaatagc agctacggct
     4141 acggctacgg caaacatcac tgcgctgacg atatctgcag tcatatctcc tgtaccttct
     4201 ccaagataaa tacgaaacag ttggatacct aaaatcaata aagacaatgc caaaatagcc
     4261 agacttagtt ttttactgaa tttatccaga cctcgttgta acggagtgac ttgctcagtt
     4321 gttgattcca aaaggccagc aacttgtccg atctccgtat tattcccagt tgaagtcact
     4381 aagaatttac cgcgtccgta agttacgatc gttccactga ataccatatt tgtacgatct
     4441 ccgattggta ccgtacttgt gatatccgtt acttcttttt ctgcagaagt cgactctcct
     4501 gtcagcatac cttcatctac ttttaatgat cccgcatcta gtaaacgtcc atcagcaggt
     4561 acataatcgc ctgcgtctaa caacacgata tctcctacca caagttcgtt tgccggaatc
     4621 gttttttcct taccatcacg aatcactcga gcatcgggcg ctgaaaggtt tttcaacgct
     4681 tctaaagaac cttctgcttt ttttgtttgg acaacactga ttatagaatt caacatcaat
     4741 actgcaaaaa taactaagaa ttcgatgtag tcacccatga ccatctgaat cagtgcgaca
     4801 atcagcaata cgataaccat tgcatctttg aaagtttcta agaacaattt ccaagttggc
     4861 ttcttcggct tttcttttag ctggttcaag ccatctcttt ctaatttttc cttcgcttgc
     4921 gtggtgctca atccatcggt tgagctctgc gtttgctgca tcaacgagtc gatcgtttct
     4981 ttatagaccg cttgttgtgc ttgttccttg ttttccaaat ttcatccccc attttttgat
     5041 cttggcccta gtcctgtctg aaaaaaaaag agacctaaga caaatacgca tttgtcttag
     5101 gtctcaccat ttcatacaat accagaaaac catttggtta acttactgtt gatattatcg
     5161 caacaaacgt tgccggttac tcccctaagg agatattcaa ctgtactaat atcctacctt
     5221 attaacctac aaaaagcaac tactttagcc cgatttacct acattttcat gaaaggagtg
     5281 aaaagtatat tcaccttggt aggcttcttt cttctatcat gctatactaa tgttattgta
     5341 tgtaaggagg aacatttttg tggtatctag tattataacc gccctttatc tattaaatgc
     5401 acttattgct ttggtggcta ttttgatcaa accccgagat gtagcagcca tttgggcatg
     5461 gctcttagta ttgtttgccc ttcctggggt gggctttgta ttatatttat ttttcggacg
     5521 tggattaacg gataagaaaa aattctatct ccgacaaagt gacttgaaag aattagaaaa
     5581 ctttcagtct tttagaggag ataccattga acattacgat cctgacatgg gcgataaaga
     5641 caaacagcaa tttgtcgact tcttctcttc attaaatcgt atgccgctga caagaatgaa
     5701 ttctgtcact cttctcacag acggacaaga gaaattggat tcactgcttc aagatctaaa
     5761 aaaagccaaa cattcgatcc atatcgaata ttacgcattt gtgacagata atatcggcca
     5821 gcaagtctta catgttttag aagaaaaagc cgcagaaggc gtggaagttc gaatattata
     5881 tgatgcattt ggctctcatg gtacaaaagc aaaagatttc aatcgtctaa tcaaaaatgg
     5941 tggacatgtc catacatttg ttacctcaca aaaggcatta cttcgtttcc gattgaatta
     6001 ccatgatcac cgaaaaatcg ttgtgatcga tggaaagatt agttataccg gtggtttcaa
     6061 tattgccaat caatatgtaa atacaacaaa aaaattcggc tattggcgcg atacgcatat
     6121 acggattttc ggtgccgctt cttctttgct ccagcttcgc ttcttaacag actggaacgt
     6181 ctcggtacct gaagaaaaaa aggtcggcta tcatttgaat tatttcttta aaaaagcaga
     6241 tcgagatgaa tctaagcttg ctgatacatc catccagctt gtttcaagcg gaccgaataa
     6301 cgaaagggaa caaatcaagc tttcatttat caaattgatt acttctgcta aaaaacgtgt
     6361 ttggatacag acaccttacc ttgttcctga tgaaagtgtc attgctgctt taaaaatcgc
     6421 aactgcctct ggtgtagatg tgaaaatcat gattcccaac aaaccggatc atccttttat
     6481 ttatcgagca acacaatatt atgctcggca gctgatcaag gaaaatgtac aaatccttgt
     6541 ctatgagaac ggcttcctcc atgcaaaaac attgataatg gatgatgaaa tctgcatggt
     6601 aggttcagca aatcaagata ttcgtagcta ccgattgaat tttgaaacaa gtgctgtcat
     6661 ttacgatcct gagtttttag aagaacttgc tactcagttc aaagaagatg agacacattg
     6721 ttcatccatg acaactgaaa cagtcaagga aatgtctaac tggctattat tcaagcaaca
     6781 aatttctcga ttattttctc caatcctata atttttttat tcaagtagag aaaaatatac
     6841 taagatt
//
LOCUS       DAKBVG010000138         6791 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000138 DAKBVG010000000
VERSION     DAKBVG010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.138"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(<1..1197)
                     /locus_tag="OUD09_001986"
     CDS             complement(<1..1197)
                     /locus_tag="OUD09_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HCT9693246.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDDSEE
                     ALQEMNLVRTKMNKSKDIAQAFHVIHSFDKSTSKELSIDKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNINMETYKRLRIDPKDLERMRKINDQVLNEHGLESVNNEY
                     YENLEKRTNLYSNDPSKMKYGKINYQEFVQNAIEATLEKCEVNSFEDFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQDVNFYERRGELITLGRDKQSRKEKSQYVERTFSARKLGK
                     RYSFESVSEQLEKNLEESHIKNFFNMEREQVMSLISGVNYKTGELNVPFVELDSIAQK
                     QFSDREGDEKFIKYDDFLREVDKHFVNTMSLEDDQEYTIKLHTDVNPQTLRSAKSKTI
                     SIKKTDQ"
     gene            complement(1182..1793)
                     /gene="mobC"
                     /locus_tag="OUD09_001987"
     CDS             complement(1182..1793)
                     /gene="mobC"
                     /locus_tag="OUD09_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HCT9693247.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVALTKQLNQIGNNLNQAVKALNIIAKYFREESEDKKEK
                     SLHFSFDASRYNIFKDELANSIAKKLMISYPDDIFLESNRSAPSFFKLSEVEKEYFEY
                     WIEKQTRPGTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            complement(1797..1994)
                     /locus_tag="OUD09_001988"
     CDS             complement(1797..1994)
                     /locus_tag="OUD09_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693248.1"
                     /translation="MQLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKNEIESNFKCCSLEKNVRG"
     gene            complement(2009..4693)
                     /locus_tag="OUD09_001989"
     CDS             complement(2009..4693)
                     /locus_tag="OUD09_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693249.1"
                     /translation="MKIIGKKIANRKINWLLISFLILIAMIIFTSNVAEGSIFNWFDD
                     TKSQTEFLTNPLYQNYLKKYNGNFYSQFGMWIGWSVIKSMFAVVDGIQNMIPEVLDLF
                     SFVESAGLNKVYQSVLSTIVVGLMILSLVIVGYKMIIGKGTVDLKSVGMNVVMSISLI
                     LLMPTLIHSGIEFSKTFYNDSTTITDSDEGVAWSLIEQGVTDLAYINKTDQYSMIDKT
                     NDRNNLTKENFWKTDLTQVLTDNVLDKMEKENPIADNLRYELVENSDNEFVATKFSDN
                     FLSTFVDGIKSGYYRYQANLWGISFGLIALAVAYVFSAFIIITAILELAFKRVLGVLV
                     FATDLETGQRSKQVLSDILQCYLTVGFQGFGLSMFAMFVNYLNAGEGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGTGGMNRSKGGKN
                     SGGNDQAEGEERKPERNFGQSLSQKANKTGKTLGYARERGLSGLASDGATMASERATK
                     PFKSMRDIANDTKNKFKEGLDDGTVSAINKNSKPIVAKNKEDETGKYADSMPTRLSDR
                     KDGAKVENADRIMSSSERMREAMKNNAESNTNHASAIQQKVKQDIEERKNAMHGQAKS
                     AEELINQKRQEAKYTPEIMNREEMLRKRVEGRTGANMDEKEALTKERIQEAKNSNTGL
                     EKLVKENLQNSTSSQDRRSVDVRENLQNTTSSQSGNRIVDVRENVQSSASNQSRTVDV
                     RENLQNTTSSQSGNRTVNVRENVLSSGTSQPRTVDVRENLQNTTSSQSGNRTVDVREN
                     VQSSTSSQGFKTVDVRENVQKDSGEMKEKAQKINIVEDRKSAKKFDSNHETVIIDSEI
                     RENDKGSKSRRRFTYEDNELFRDTLNDPNPLFDNLLKK"
     gene            complement(4918..5787)
                     /locus_tag="OUD09_001990"
     CDS             complement(4918..5787)
                     /locus_tag="OUD09_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaD"
                     /protein_id="HCT9693250.1"
                     /translation="MAEKRMLSKTFLNNASFMKMSDSAKLLYVYLNTNADDDGIVEAY
                     PYVKMLGTPDDDVKILFTKGFVFFLNEDLLAFLPHWLQNQNIRIDRYKPSIYRFKLLN
                     LKPEFFSKLKPIKLDVILKSGQVEPLRVDLMEKWYPVGIPSINHLEVQSSQDQISSDK
                     SSLVKNSIDKINQVDTIINEEIINEKGIQLIHKLGDRFTKSQREKFVQLIVDMKNSTE
                     REFKIRIDGPHYYEQTNKMIIRLFNTINEKLNLGKELNEMKYVNTVSRNHWKEIALEE
                     QDNRMYIIDALSK"
     gene            complement(5941..6243)
                     /locus_tag="OUD09_001991"
     CDS             complement(5941..6243)
                     /locus_tag="OUD09_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCT9693251.1"
                     /translation="MEAIKLMISEEQEEMFLKQFYQIATKAIEKAQENAGMTREYLNQ
                     KEMAEYLNVSENFLKNAVRNEGLPVVELGARKFYSKKAVNEFMFARMKRGESFERK"
     gene            6445..>6791
                     /locus_tag="OUD09_001992"
     CDS             6445..>6791
                     /locus_tag="OUD09_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCT9693252.1"
                     /translation="MKPNPSEAGKRIKQLRLSCGFTMEELGRKIDNSPRATISNWERG
                     TNLPNPQKLKLLSTITNSTIDWIKWGTLEEYITSYLIDIGYELYIKDFPEIPHKVFKD
                     IQERYSDTFSLDK"
BASE COUNT         1989 a         1326 c          905 g         2571 t
ORIGIN      
        1 ctgatcagtc tttttaatag aaattgtttt acttttcgca ctacgtaaag tttgaggatt
       61 cacatccgta tgaagtttaa tcgtgtattc ttgatcatct tctaaagaca ttgtatttac
      121 aaaatgcttg tctacctctc gcaagaagtc atcatattta atgaattttt catcgccttc
      181 acggtcgcta aattgctttt gtgcgatgga atctaattcc acaaacggta catttaactc
      241 accagttttg taatttactc cactaattag cgacataact tgctctcttt ccatattaaa
      301 aaaatttttt atatgtgatt cttctaaatt cttttcaagt tgttcagaaa cagattcaaa
      361 tgaatatctt ttccctaact ttcttgcact aaaagtacgt tcgacatact gacttttttc
      421 ttttctagat tgtttatctc ttcctaaagt aattaactca cctctacgct catagaaatt
      481 tacatcttgt ttgtaaagaa catagccaag tttgttattt gtttttgaaa attgataaac
      541 ttcaatgtta tattcttttg ccaaatgatc cgaaaaatct tcaaaagaat tcacttcgca
      601 tttttccaac gttgcttcga tcgcattttg cacaaactct tgataattaa tttttccata
      661 tttcatctta gaaggatcgt ttgaataaag attggttctc ttttctaagt tttcatagta
      721 ctcattattc actgattcaa gaccatgttc attgaggact tgatcgttaa tttttcgcat
      781 tcgttctaaa tctttagggt caatacgcaa ccttttatat gtttccatgt taatattatt
      841 aatgacaaga tgactatgaa aatgatcttt atcattgtga cttgcaacaa tgatttgcga
      901 atttggaaaa gctttctctg caaattcgac agctatttcg tgcattttat caatacttaa
      961 ctctttagaa gtactcttat caaatgaatg aataacatga aacgcttgtg ctatatcttt
     1021 agatttattc atcttggtac gtactaaatt catttcttgc agtgcttcct cactatcatc
     1081 actacaattt attcctgaga ctgcaacaca tttttctttt tttgaaggat tagctgcata
     1141 ttgtaatagc gaggataaat ttttcttgcg tcctttcgat gttatagtag ctcccaaagt
     1201 tgtttcacct catcttttaa acttgcaata atctgaattg agtgaattaa attccgatca
     1261 actttttggg aatactcttc tttcgtaccc ggtctagttt gcttttctat ccaatattcg
     1321 aaatattctt tttctacttc tgataattta aaaaaagagg gagcactccg attggactcc
     1381 aaaaaaatgt catctggata actaatcatc aacttttttg ctatgctatt cgcaagttca
     1441 tctttaaaaa tgttgtaacg gctagcatca aaactaaaat gtaggctctt ttctttttta
     1501 tcttcagatt cttctctaaa atatttagca atgatattta atgcctttac cgcttgattc
     1561 aaattgttac caatttgatt taactgtttg gttaaagcta ccctatcatc ttcagctaac
     1621 aacggcggtt catatttgag tccctttaac cgctcacggc aaacgtctgc tctagaaatt
     1681 cccctttgtt ttgcaatcaa tgtcagttca agataagtct ttttatcgac acgaaaattt
     1741 aaggcctcac tatgtttgcg atttttttcg tacatttttt gtttatcatc cattgattat
     1801 cccctcacat ttttttccag agagcaacat ttgaaattgc tctctatttc atttttcatc
     1861 tttaattctt cttgaatcat cagttcaata taattagtga ccgtgatttt ttcatttgtt
     1921 gcgttcttta ctgattcaca taattcgtca agttctaaga aatcatgcgt actaatcttt
     1981 agaattaatt gcatgacaat cccccctttt atttttttaa tagattatcg aaaagtggat
     2041 taggatcatt aagtgtatct ctgaataact cattgtcttc ataagtgaat cttcgtcttg
     2101 attttgatcc cttatcattt tcacggattt cactatcaat aatcactgtt tcatgatttg
     2161 aatcaaattt tttcgctgac tttctgtctt caacaatatt gattttttgt gctttttctt
     2221 tcatttctcc actatctttt tgaacgttct cccgaacatc tactgttttg aatccttgac
     2281 tacttgtaga actttgtaca ttttctcgta cgtctactgt tcgatttcca ctttgactac
     2341 ttgtagtatt ttgcaagttt tctcgaacat ctactgttct cggttgactt gtcccactac
     2401 ttagtacatt ttctcgtaca tttactgttc ggtttccact ttgactactt gtagtattct
     2461 gcaagttttc tcgaacatct accgttctcg attgatttga cgcactactt tgtacatttt
     2521 ctcgtacatc tactattcga tttccacttt gactacttgt agtattctgt aagttttctc
     2581 gaacatctac tgagcgtcta tcttgactac ttgtagaatt ttgtagattt tctttgacca
     2641 atttttctaa tccggtattt gaatttttag cttcttgaat tctttctttg gtcaacgctt
     2701 ctttttcgtc catattagcc cctgtacgcc cttctacacg tttcctcaac atttcctcac
     2761 gattcattat ttcaggcgtg tattttgctt cctgtcgctt ctgattaatt aattcttcag
     2821 cagacttagc ttgaccgtgc atagcatttt tacgttcttc aatatcttgt ttaacttttt
     2881 gctgaattgc acttgcatga ttagtatttg attcagcatt atttttcatt gcttcacgca
     2941 tacgttcaga actagacata attctatcag cgttttcaac tttagcgcca tcttttctat
     3001 ccgaaagacg cgttggcata gaatcagcat attttccagt ttcatcttct ttatttttag
     3061 ctactatagg tttgctattt ttattaatag cggacacggt accatcatct aaaccttctt
     3121 tgaatttatt cttagtatca ttagctatat cacgcatact cttaaatggt tttgttgctc
     3181 tttcactagc cattgtagct ccatccgaag ctaagcctga taatccacgt tcacgagcat
     3241 acccaagcgt tttccccgtt ttattagctt tttgagaaag tgattgacca aagttgcgtt
     3301 caggttttcg ttcttcccct tccgcttgat cgtttccacc actatttttc ccacctttag
     3361 atcggttcat accacctgtt cctttacgaa acagcatagc tcccatacca aatgctgaag
     3421 caagctggcc atagccttct tttaaaccaa tatccacgcc aaagtaacgc atgactgttc
     3481 cactaccctt tattagaacg aacacggcac agatataagc aattgttttt ataaaaatat
     3541 tcgttgagat accttcacca gcgtttagat aattaacaaa cattgcaaac atagacaatc
     3601 cgaatccctg aaaaccgaca gtcaaataac actgtagaat atctgacaaa acttgttttg
     3661 atcgttgtcc tgtttctaag tcagtagcaa ataccaaaac tccaagtacg cgtttaaatg
     3721 ctaactcgag tattgctgta attataatga aagcactaaa aacatacgct actgctaaag
     3781 caatcaatcc aaatgaaatt ccccacaagt tcgcttgata gcgataatat cctgatttga
     3841 taccatcaac aaaggttgac aaaaaattat ctgaaaattt agtagcaaca aattcattat
     3901 cagaattttc aactaattca taacgcagat tatcagctat tgggttctct ttttccattt
     3961 tgtcgagtac attatctgtc aatacttgcg taagatccgt tttccaaaaa ttttcttttg
     4021 ttagattgtt ccggtcattc gttttatcga tcatggaata ttgatccgtt ttattgatat
     4081 aagctaaatc agtaacccct tgttcaatca gcgaccacgc cacgccttcg tctgaatctg
     4141 taatcgtagt tgaatcatta taaaaggttt tagaaaactc tattccgctg tgaatcaaag
     4201 ttggcattaa aagaatcaat gaaattgaca ttacaacatt cattccaaca gattttagat
     4261 caaccgttcc tttacctata atcattttat aaccaacaat cactagtgaa agtatcatta
     4321 agccaacaac aatggtactt aaaactgatt gatagacttt gttcaatcca gcactttcca
     4381 caaagctaaa caaatctaaa acctcgggaa tcatattttg aatgccatcg acaacagcga
     4441 acattgattt tataacagac cagcctatcc acataccgaa ctgactgtaa aaatttccgt
     4501 tatatttttt aagataattc tgataaagtg gattagttaa aaactcagtt tggctttttg
     4561 tatcatcaaa ccagttgaaa atactgcctt ctgcaacgtt actcgtaaaa ataatcatag
     4621 ctatgagaat aagaaaacta atcaacaacc aatttatttt tctattggca atcttttttc
     4681 caataatttt cattgttacc tccctcagcc cagtttctga ggctctcaga gagcttttga
     4741 gcaatttttt ggtcaatgat gatatgagtt acataactat cattcactgc tcttaaatcg
     4801 agaacaatag tgttgaagca gatacactcg attaattcaa gtgtatctgc gttttggtca
     4861 agacagcaga tttgatctat tagacgattt ccaatcatat taagagtcct ttcttgttta
     4921 tttacttaga gcatctatga tatacattct gttatcttgt tcttctaaag caatttcttt
     4981 ccagtgattt cgagataccg tgtttacata cttcatttca ttcaattctt tacccaagtt
     5041 taatttctcg ttaatcgtat taaataatct gattatcatc ttatttgttt gttcatagta
     5101 atgtggccca tcgattcgaa ttttgaactc tctttctgtt gaatttttca tatctactat
     5161 taattgaacg aatttttctc tttgactttt agtaaatctg tcacctagtt tatgaatcag
     5221 ctgtattcct ttctcgttaa ttatctcttc attgataata gtatcaactt gattaatctt
     5281 atctatacta ttcttaacta aactagattt atctgaacta atctgatctt gacttgactg
     5341 aacctccaaa tggttgatgc taggtatacc aactggatac catttttcca ttaaatcgac
     5401 tcttagcggt tctacttggc cagatttcaa aatcacatca agttttattg gctttaattt
     5461 tgagaaaaat tctggtttta agttcaataa cttgaagcga taaatagatg gtttatacct
     5521 atctattcgt atattttggt tttgtaacca atgcggtagg aatgctaaca aatcttcatt
     5581 taaaaaaaat acaaaacctt ttgtaaaaag tatctttaca tcatcgtcag gagtcccgag
     5641 cattttcacg tatggatatg cttcaacaat tccgtcatcg tctgcatttg tgttcagata
     5701 tacatataat aattttgctg aatctgacat cttcataaaa cttgcattat ttaaaaatgt
     5761 tttggacagc attcgttttt ctgccatgga tttcacactt ctttctattt gaatggtaaa
     5821 ataaattcaa ataaagtttt atcagaactt tattgttggg tgtttccctt cgtctacaaa
     5881 ctatcgggag cactctttta tatatttttt tcaattcttt tcaagtctaa atttgttatt
     5941 tcattttctc tcaaaacttt cacctctttt cattcgagca aacataaact cattaactgc
     6001 ctttttcgaa taaaattttc ttgcccctaa ttcaacaacg ggcaatccct catttctcac
     6061 cgcatttttt aaaaagtttt cgctcacatt taagtattct gccatttctt tttgatttag
     6121 atattctcta gtcatacccg cattttcttg agccttctca atcgcttttg tagcaatttg
     6181 ataaaactgc tttagaaaca tttcctcctg ttcttctgaa atcattaatt ttattgcttc
     6241 cacaaaaacc tctccttttt aattaattat tctataactc ctctaccttt ataagtacaa
     6301 ataaatacaa attgcgtaaa aaaatgtatt tatttgtatt tataagaata aagtatcata
     6361 ctccagataa aaagtaaata cttttaaata cttttatttg ataaatatga tatactatct
     6421 ttaaaaataa aaaggagaaa aaagatgaag ccaaatcctt cagaagcagg aaaacgaatc
     6481 aaacaattac gcctttcatg tggttttact atggaagaac ttggaaggaa aatagataat
     6541 tcaccacgtg cgaccatatc caactgggaa cgtgggacca atttaccaaa tccacaaaag
     6601 ctgaaattat tatcaactat cactaattca acaattgact ggattaaatg gggaacttta
     6661 gaagaatata taacttcata ccttatcgat atagggtatg agctatatat taaggacttt
     6721 ccagaaatac ctcacaaagt ctttaaagac attcaagaaa gatatagtga tacgttttcg
     6781 ctagataaag a
//
LOCUS       DAKBVG010000139         6783 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000139 DAKBVG010000000
VERSION     DAKBVG010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6783
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.139"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            16..906
                     /locus_tag="OUD09_001993"
     CDS             16..906
                     /locus_tag="OUD09_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HCT9693253.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(979..1803)
                     /gene="nadE"
                     /locus_tag="OUD09_001994"
     CDS             complement(979..1803)
                     /gene="nadE"
                     /locus_tag="OUD09_001994"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCT9693254.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(1815..3287)
                     /locus_tag="OUD09_001995"
     CDS             complement(1815..3287)
                     /locus_tag="OUD09_001995"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCT9693255.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(3924..4286)
                     /locus_tag="OUD09_001996"
     CDS             complement(3924..4286)
                     /locus_tag="OUD09_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HCT9693256.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            4509..5261
                     /locus_tag="OUD09_001997"
     CDS             4509..5261
                     /locus_tag="OUD09_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCT9693257.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(5401..6324)
                     /locus_tag="OUD09_001998"
     CDS             complement(5401..6324)
                     /locus_tag="OUD09_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCT9693258.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(6339..6611)
                     /locus_tag="OUD09_001999"
     CDS             complement(6339..6611)
                     /locus_tag="OUD09_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693259.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT         2071 a         1350 c         1161 g         2201 t
ORIGIN      
        1 taagaggtga tatatatgca actacaaata aacgaagaat ccttaccggt atatgaagca
       61 ttagctagta aaacaaggat caagatcatc cagctacttt ccaaaaaaaa gatgaatgtc
      121 aaagatttgg caaaagaact tggagtgagc agtgctatca cgacgatgca cgtgaaaaag
      181 ctagaggaag caaacatcat caaaacagaa aaagtaggac aacaaaaaat ttcgagcctt
      241 cgtgtcgata aaatcgatat cagttttcca gaaaaaatat tcaatgcttt tgatacgaaa
      301 gaaacatcga tacctattgg tcactataca aattatgcaa ttgaaccaac atgcggttta
      361 gctacgatcc atgattttat cgggaaagtg gatgaaccac gctattttat ggatcctcga
      421 aggatggatg cccgcattct ctggtttacc agcggatttg tcgagtatca ggcacctaat
      481 tttttaaata cagaggatac attggagatg ttggaagttt ctgtggaaat ttcttctgaa
      541 tttccttttt caaatgacaa ttggccttca gatatcactt tcagcctaaa tggtgtggag
      601 ctaggaacat ggacgagccc tggagatttt gctgatatcc gcggtaaata tacgcctgat
      661 tggtatccag ataatcttaa tcaatacggc ttgctgaaga cgatccgaat taccaaacac
      721 ttgacgaata tgaatggaga acccttgtca aacatcacga tcaacgacat ccctaaagag
      781 caagacactt ggcatttgcg aatcgaagtg aaagacgatg ccaaacatgt ggggggctgt
      841 actttatttg gtaaaggatt cggtaattac gatcaggata tcaaattaaa agtttactac
      901 agctgatatt tttcttttct attttataaa acaaaaacag ccaatcccta tgaagggacg
      961 gctgtttttg ctttattgtt atttccagaa atcatccaaa acggaaattg gcaaatgtcg
     1021 tttatgttgt gtcttgttcc accatgcttc gattttttct tgtgcttgag cgggaacttc
     1081 ttttccttct agataatcat caatttcatt atatgtgaca cctagtgcga cttcatctgc
     1141 aatcattggt ttcccatctt caagatctgc agtcggaatt tttgtgtata atttttccgg
     1201 tgcattcaat gcttgaagca attgtttccc ttgtcgtttg tccaaacgga ataatggcaa
     1261 aatgtcggct ccaccatcac caaatttcgt gaagaatcca gtaatgtttt ctgcagcatg
     1321 atctgtccct aatacggctc ctgctctttc tccagctacg gcatattgcg taatcatacg
     1381 ttgacgtgct ttgatatttc ctttgttaaa atcgctgatt cccacacctg cttcgcttaa
     1441 tacttgtact tgtgcatcaa cagctggttt gatatttact cgcagactaa catcgggctg
     1501 gataaagtca agagcagctt ttgcatcttc ttcatctgct tgttcaccat aaggaagacg
     1561 aacagcgata aattgatagt tttcatcttt tgtttcttca cgcatttctt ccatcgttag
     1621 ttgcgccaaa cggcctgcta aagtagaatc ttggccgccg ctgataccta gtacaaaagt
     1681 tttaagaaac ggatattttt tcaaatatgc tttcataaag tcaatacttt tacgtatttc
     1741 ttcttttgga tcaattgttg gtttgacacc taattcattg ataatttctg tctgcaggct
     1801 catttattct cctcctatgc ttctgtatga agttctgcta cgcttttttt cattcgttcc
     1861 atgatagcca ttttatggtt ccagcattca gtagacagat cgactgggta tttttgcgga
     1921 ttcaagtctc gtttatactc ttcccatagt gaatctagat tttcttttgt atattctttg
     1981 atttcgttta gtgcaggtaa ttcgtaaact ctttttcctt cgataaagat atcttgtaaa
     2041 actgggcgag ctgtaaagtc tcgaacagtt ttattgataa acgtatgcac tggatggaac
     2101 atgaagatag cttcttcttc tcgagggtct tcatcccata atgtcacgta gtctccttca
     2161 gatttcccat caaaatttcg agtgatgcgc cacacttgtt ttttccctgg agtagttact
     2221 ttttctgcat tgcttgataa cttgatcgta tccatcattt ttccttcatc atcttcgatc
     2281 gatactagct tgaatactgc accaagggct ggctgatcat aggctgtgat caattttgtc
     2341 ccaacacccc atacatcaat tttagctttt tgcatcttta aattcaaaat cgttgcttca
     2401 tccaagtcat tcgaagcata gatttttgct tccgtaaatc cggctgcatc taattgttcc
     2461 cgaacacgct tagatatata ggccatatct ccactgtcga tgcgtacgcc ttggaagttg
     2521 attttgtcac ctaattcttt cgccacgcga atcgcacttg gcacacctga tttcaacgta
     2581 tcataagtgt cgactaagaa cacgcagtct ttatgggttt tagcgtaggc cataaatgct
     2641 tcataatcat ttccataaga ttggactaac gaatgagcat gggtcccgct agctggtatc
     2701 ccaaatatct tcccagcacg tacattgctt gttgcatctg ctccgccgat atatgccgct
     2761 ctagttcccc agactgctgc atccagttct tgtgcccgtc tagttccaaa ttccaatagt
     2821 ggatcttctc cgatgacaga tttgatacga gctgcttttg tcgcgatcaa cgtttggaaa
     2881 ttgaccatat tcaacagtgc tgtttctact aattggcagt gcgctaaagg cccttccact
     2941 tggattaatg gttcatttgc aaaaactagt tctccttcac gagcagaacg aactgttgct
     3001 ttaaattcaa aattttgtaa atactccaaa aaaccttcag gatagacttc taatccacgc
     3061 aagtaagcaa tatcggaatc attgaacgtc agattctcta aataattgac taaccgttcc
     3121 aatcctgcaa aaaccgcgta tccgtggtta aaaggcattt ctcgaaagta acattcaaaa
     3181 acagcatgta aatcagctcg acctaattcc cagtatgtct gcatcatatt gatttgatat
     3241 agatccgtat gcagcgtcaa actgtcgtct gggtaagtat tattcatctt gtattactcc
     3301 tcttggcaag ttttcatttc ctacccatta taacaaaaaa aattaatagg aaaagaaatc
     3361 ttccattttt tctgaaaaag cacatgtttt acggatattt cttgctaaaa atgaactcca
     3421 tctgctaaaa ctatcctttc ttattggtat ttaacctaaa cgagaaagaa tacttatttt
     3481 attcaatgta tccgagacac gaagaaatta gcgttttcta tttttcttac ttgataaaat
     3541 aagtagattt tcctactaca atcgtagccg ttaagatacc gatgattgaa caaatataac
     3601 caaatgtctc tacaacataa tcaatggcaa aagtttttga agcaataata cgtctttcct
     3661 tttttgcttc ttatccccaa agaaacggat ataacttatt atgtattgaa tttttttgga
     3721 aaggcgaatt atgacaagat ataccttgcg ttgaaaaaac tcgcggaaaa aatatcacac
     3781 gtttttaaaa agatacaaag taagaaagca gctgtcaata gaatggaaac aaaaatggcg
     3841 agacatcaat cagtctcgcc ttgtttttta ttgtatacat tagatatgga gcaggttacc
     3901 ataaagatga ttattagtcg ttattaaata gaatccttcg tatttttttg ttctatcact
     3961 acaatatctt tatttggacg tttgaattgt attccggcac gttctagctc ctgtttcact
     4021 cctacatgga ctttgacatc ttctcgtgtc atttttaaaa cgtgatgaat cgcatagtcg
     4081 atttcttgcc ttgtgatttt tttcttcgta ttgatcatca cgacttgctt tttatcatat
     4141 gtgtctacca atagcagttg tgttcgatct aacgaaaata atttgtaata attaatagcc
     4201 gtattcccaa aaaagaattc cactacttta ggataaaatg tcttaatatt taaattacta
     4261 ttcacaggtg tttcgattag cttcatctca ttcctctttt ctatatagtc gatttagatc
     4321 caatcttaag ttatgttacc atctttccta gcattaagga atcggttaca attgaacctt
     4381 taaagacaaa aggatttacc ttttatcact taatagtata acataatttc gtgagaaaaa
     4441 aatacaattt tttcttgacc atctcgttac gtaaaggatt atgcttacat ttgtcaggag
     4501 gaaaaataat ggagtacaca ataaagaaaa tgtcagagat atcaggagtc agtccgagaa
     4561 ccttgcgctt ttatgatgag attggactgc taaaaccagc taggatcaat tcttcaggtt
     4621 atcggattta tggcaaaaaa gaagtcgatc gtctgcaaca catcttattt tatcgaacaa
     4681 tggcattcaa gttggatgat atccaagagg tcttagacaa tccatctttt gaccaccaga
     4741 aagctttaat caagcatcag caaatgttgc tggaaaaacg ggcacaaatc gataccctct
     4801 tgacaactgt ccaacagaca cttgatatgt acgaaggagg aagaaaaatg agtgacaaag
     4861 aaaaatttga agggtttaaa caacaaaagt tgaaagaaaa tgaagaatcc tatggacaag
     4921 agattcgaca aaaatacgga aaaaaaaagg ttacagcatc caatgaaaaa tggatgaata
     4981 tggataaaga aacgtatgaa gaaatgcaaa ctgttgaaaa gcagatgtta tttgatctca
     5041 ctctttactt aaaagaacca gaagatgaaa gcttagcaga ccgtatcttc caagctcata
     5101 agaagtggtt ctcttacagc tggcctgaat atcagccaaa agcgcataaa ggactaggaa
     5161 tgatgtacga agccgatgaa cggttcactg catattatga cgagcgaagt ggtcatggtg
     5221 cagcaaaagc tttaaatgaa attatccaaa aaaaggcgta atcttttaca tgatccaaca
     5281 aaaaagtaca cgtaaaatga cctaataaca aaacagcaga cacccaagtt agatttttat
     5341 gctaactttt ggtgtctgct ccattctttc acaagttttt tcatcctact ttaaatatat
     5401 tcatcgttga ataaacgtat aaggtatttt cactttatcc tttttttctg taagaaacaa
     5461 ttgaaatgcc atttctccgc aagcttccag atggtggtcg atggttggga aaccaagaag
     5521 ctcgctgatc agcaaattat cgcgtccaat caccactggt gtcttatctt gcagatagct
     5581 ttgcaagatg cctgctgcaa ccatatccga atttgtaaag acaccatcaa ctccaagctc
     5641 tttaaaaaag aagcctgctt gttttccttt ctctgcatct atacaatccc aaaaaacaag
     5701 tgattcatca aaagatggaa aaagttgatg acatagctgc aaagtgattt ttgagttata
     5761 acttagcctg ccacttcgcc ccaacgtgat gcccaattgc tttatacctt gctgcttcaa
     5821 ataagtaaga ccttctgtaa ttgattgttc tcgatcaata aagacacagc ttgcctgttt
     5881 tcccttgatt tcttcacaaa aaactagagg tccatatttt tgatatgcca ataaatcatc
     5941 tagtgtattg gcacgagacg taatgatcaa tccatcgaaa gctttagctg caaattcttc
     6001 aagatacctt cgctcttttt ctttatgata gcccgtcggt aaaagtgtca ctttgtaatc
     6061 ataatcaaac gctgcttcaa tgatcccact gatcaactga tcaaaatacg gatgattggt
     6121 ataaggcaga atcactccaa tgcttttcgt ttttccataa cttaactgtc tggcatttgc
     6181 attagggata taatcgactt ctttcatgac cgcaagtatt tctttccttt tttcctcatc
     6241 tacatatgga tgtccattga ttacacgtga aatagtagaa actgaataac ctgtgagctt
     6301 tgcgatatct cgaatgtttg ccactgctgc ttcgccacct atacaaaaag attttcttgt
     6361 aaatagtcta ccacagctgg accgcattca aaaaactgat acatttttcc ttcatgcgaa
     6421 aaactgatcc gctgcccgcc ccaatatata tcttttgtca aattttcgat ttcattcatt
     6481 ggtatttctt ttactatatt cctattcaac ccttgaaaac tgtacaacgt taatacggat
     6541 cccttgaact cgacaaacgc ataaaaataa ttgttcgttg tgccatctgt tatctctaca
     6601 taagtattca ttgcaagccc tccttttgat actttgagta tagtgtatga aacgcgtctc
     6661 atagcaagga gtttgttttg ccaaagaaaa gttttattcc tctttcatta ttaccaaagt
     6721 ctcttatttt aaattgaata cattataatg atactgtaaa tatatcttta ttattagtga
     6781 atg
//
LOCUS       DAKBVG010000140         6684 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000140 DAKBVG010000000
VERSION     DAKBVG010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6684
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.140"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            8..81
                     /locus_tag="OUD09_002000"
     tRNA            8..81
                     /locus_tag="OUD09_002000"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42..44,aa:Asn,seq:gtt)
     gene            121..209
                     /locus_tag="OUD09_002001"
     tRNA            121..209
                     /locus_tag="OUD09_002001"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:155..157,aa:Ser,seq:gga)
     gene            224..295
                     /locus_tag="OUD09_002002"
     tRNA            224..295
                     /locus_tag="OUD09_002002"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:257..259,aa:Glu,seq:ttc)
     gene            311..383
                     /locus_tag="OUD09_002003"
     tRNA            311..383
                     /locus_tag="OUD09_002003"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:344..346,aa:Val,seq:tac)
     gene            393..465
                     /locus_tag="OUD09_002004"
     tRNA            393..465
                     /locus_tag="OUD09_002004"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:426..428,aa:Asp,seq:gtc)
     gene            501..576
                     /locus_tag="OUD09_002005"
     tRNA            501..576
                     /locus_tag="OUD09_002005"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:534..536,aa:Phe,seq:gaa)
     gene            578..658
                     /locus_tag="OUD09_002006"
     tRNA            578..658
                     /locus_tag="OUD09_002006"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:612..614,aa:Tyr,seq:gta)
     gene            665..738
                     /locus_tag="OUD09_002007"
     tRNA            665..738
                     /locus_tag="OUD09_002007"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:697..699,aa:Trp,seq:cca)
     gene            741..813
                     /locus_tag="OUD09_002008"
     tRNA            741..813
                     /locus_tag="OUD09_002008"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:774..776,aa:His,seq:gtg)
     gene            825..896
                     /locus_tag="OUD09_002009"
     tRNA            825..896
                     /locus_tag="OUD09_002009"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:857..859,aa:Gln,seq:ttg)
     gene            942..1012
                     /locus_tag="OUD09_002010"
     tRNA            942..1012
                     /locus_tag="OUD09_002010"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:974..976,aa:Cys,seq:gca)
     gene            1033..1117
                     /locus_tag="OUD09_002011"
     tRNA            1033..1117
                     /locus_tag="OUD09_002011"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1067..1069,aa:Leu,seq:caa)
     gene            1179..1251
                     /locus_tag="OUD09_002012"
     tRNA            1179..1251
                     /locus_tag="OUD09_002012"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1212..1214,aa:His,seq:gtg)
     gene            1261..1332
                     /locus_tag="OUD09_002013"
     tRNA            1261..1332
                     /locus_tag="OUD09_002013"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1293..1295,aa:Gln,seq:ttg)
     gene            1350..1434
                     /locus_tag="OUD09_002014"
     tRNA            1350..1434
                     /locus_tag="OUD09_002014"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1384..1386,aa:Leu,seq:caa)
     gene            1689..2564
                     /locus_tag="OUD09_002015"
     CDS             1689..2564
                     /locus_tag="OUD09_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCT9693260.1"
                     /translation="MKESASCFVLSYSGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAIEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            2668..3432
                     /locus_tag="OUD09_002016"
     CDS             2668..3432
                     /locus_tag="OUD09_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HCT9693261.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            3419..4861
                     /locus_tag="OUD09_002017"
     CDS             3419..4861
                     /locus_tag="OUD09_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCT9693262.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAIFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSGFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            4872..6202
                     /locus_tag="OUD09_002018"
                     /pseudo
     CDS             4872..6202
                     /locus_tag="OUD09_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
     gene            6215..6673
                     /locus_tag="OUD09_002019"
     CDS             6215..6673
                     /locus_tag="OUD09_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCT9693263.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
BASE COUNT         2097 a         1100 c         1484 g         2003 t
ORIGIN      
        1 atgttattcc ggcatagctc agttggtagt agcgcatgac tgttaatcat gatgtcgtag
       61 gttcgagtcc tactgccgga gttctcattt tgagaaagag tattgagctt ctttcactct
      121 ggagagttgt ccgagaggcc gaaggagcat gattggaaat catgtagatg gtgcaaactg
      181 tctcaagggt tcgaatccct tactctccgt agcaaattta cctggcccgt tggtcaagcg
      241 gttaagacac cgccctttca cggcggtaac acgggttcga atcccgtacg ggtcatttta
      301 attgaatgtt ggaggtttag ctcagctggg agagcatctg ccttacaagc agagggtcag
      361 cggttcgatc ccgttaacct ccataatgaa ctggttccgt ggtgtagggg ttaacatgcc
      421 tgcctgtcac gcaggagatc gcgggttcga ttcccgtcgg aaccgtcgtt cttgttattc
      481 aattaataat tttaaaatat ggcgcggtag ctcagttggt agagcaacgg attgaagctc
      541 cgtgtgtcgg cagttcgatt ctgtcccgcg ccaccatgga ggagtagcga agtggctaaa
      601 cgcgacggac tgtaaatccg ttccttaggg ttcagtggtt cgaatccact ctcctccatt
      661 ttataggggc atagtttaaa ggtagaacag cggtctccaa aaccgttagt gtgggttcaa
      721 ttcctgctgc ccctgccatg gcgaatgtgg cgaagtggtt aacgcaccgg attgtggctc
      781 cggcattcgt gggttcgatt cccatcattc gcccttttta ttcttggggt atagccaagc
      841 ggtaaggcaa gggactttga ctccctcatg cgttggttcg aatccagcta ccccagtttc
      901 tataatttat tctttatttt gtagattgat aatagtataa tggcggtata gccaagtggt
      961 aaggcagagg tctgcaaaac cttcatcacc ggttcaaatc cggttaccgc cttagtacta
     1021 ataaatcaac ttgccggcgt ggcggaattg gcagacgcgc tggactcaaa atccagtgcc
     1081 cgttgagggc gtgccggttc gaccccggcc gccggtattg actaagatgt cttcatctta
     1141 gttttttatt gagaaattaa ataacatctt ttatgatggc gaatgtggcg aagtggttaa
     1201 cgcaccggat tgtggctccg gcattcgtgg gttcgattcc catcattcgc cctttattct
     1261 tggggtatag ccaagcggta aggcaaggga ctttgactcc ctcatgcgtt ggttcgaatc
     1321 cagctacccc agttattatt tttaaatttg ccggcgtggc ggaattggca gacgcgctgg
     1381 actcaaaatc cagtgtccgt tgaggacgtg ccggttcgac cccggccgcc ggtataatta
     1441 tcaatatagc aactttctta tagagagttg ctatattttt tttgttttta tgtaattacc
     1501 tccaaatcaa ttcgatacaa gtattatcat aaaacgagta aaaaagatat tgataacgct
     1561 tttattatgt gttaaactat ttttgtaaat tatgtagggt gctactattc aattaggcac
     1621 gacctaagaa agggttcttt cttaggtcgt tttttgtctt taaaaattgt ttggagacaa
     1681 aaatatgtat gaaggagtct gcaagttgtt ttgttttatc atatagtgga ggtgaattaa
     1741 cggtgtatgt gatgaaaaag ctgaatcata atgctgtatt gatcgatgac gatggaatag
     1801 aaaaaattgc gatggggaaa gggattggct tttctgctaa agtgagtaag ccatttgatg
     1861 ttgaggatgc tgacaaatta tttgtattag attctaaaga aaagatcaaa cgattcagtg
     1921 agatagtctc gcagattcca atcgaattta tggattttgc ggaagaagtg attgaactga
     1981 taacagctag gatctcctct ccattagatt caaatatcta tattacattg acagatcata
     2041 tttattttgc tattcaacga aaaagagaag atagagatgt cacagcaatt atgttgccag
     2101 aaatgagatt cctttatcca aatgaatatg atatagcaat agaagtggtc gagcgaatca
     2161 atcatcgata tcaaaccgag ttagggtcaa atgaagtagg attcatcact atgcatatca
     2221 ttaatgctga gctaggagag cctaatagtt taaagagctt aaaaatagta gaaatgacga
     2281 ccacgatttt gtcccatcta gaaaaatcct attcaaacgt ttatgataaa gaatcactga
     2341 cttatcatcg cttaatggtt catatcaaat tcctggttaa acgcttgatt tatcaggaag
     2401 aaacgccagt agaaaatatt ggttttttta ctgaaacatt taaacatagc aaagcgtatt
     2461 gtacagcact aaagattaaa gatatcatcg aacagcaatt tcaacttgcc attcaagaaa
     2521 atgaaatcat ctatttggca attcatctag cacgaatcta ttaaaataag ggcgtaacta
     2581 tctataggcg caaccaaatg acacatagta aatggactat gattatttgg ttgcgcctat
     2641 tttagtagaa aaaagaagga gaatgaaatg ggaaaagtga tttttaattc ggatgatttt
     2701 ggctatagcc atggtgtgaa ttatgggatt atggatgctt atcagcgagg aattttgaca
     2761 tcaactacat tgatggcaaa tatgccgggt ttcgagcacg ctgtgaaact aagaaaggaa
     2821 atgcctaggc taggtgtagg agtccattta acattgacgt gtggcaaacc acttctcaaa
     2881 actgtcgata ctcttatcga aagcggagag ttcagatcct tatcttatta tcagcatcca
     2941 tttaacattg acaaagatca actgtatcag gaatggaatg ctcagattca aaaaatttat
     3001 catgcgggga tcataccaac tcatttagac agtcatcatc atactcatac tttggatgaa
     3061 aaccaggagg tagttgttac attagcaaaa aaatatgatt tgccggttcg gagcaacttt
     3121 gaacgaaaag atgaagttcg tcatgtcgat tattttgagc cttgtttcga tgtcgtagga
     3181 gaaactgatg aaaaaaaacg acaaatcaat acgagtgtgg aagaatattt ggcaagttta
     3241 ctcatgatat tgagagaaga taaaacgatc gaagtcatgt gtcacgcagc gtatttggat
     3301 caggaattgt taaatggttc aagttttgtg tatcctcgta ctcaccaagt cgaattttta
     3361 attcattcag catttgctca aaaagtgaaa aatgatcctt tgattgagtt agtaacatat
     3421 gaggatattt aatagaggag gaataaaaat gaatgttaaa tcaaatgtgc agaagcttgg
     3481 aagagcgatg ctacttccag ttgcagcgat gccattagcg ggattgatca tgcgtttatc
     3541 agctgatgat atgctgaaca tcccagtaat tggtgcagct ggaaatgccg tttttggaaa
     3601 cttggatatc ttatttgcta taggagttgc aatcggtttt gtcaaaaata aggacaaagg
     3661 aatacctgca cttactggtt ttttagcgat tgctaccttg aaaaaaggct tagaaatcat
     3721 ggacccagaa gtgaatatgg ggatttttgg tggaattatt tccggtttag tagcggcatg
     3781 gacgtataat cgctttaaag aacaaaaatt accagtagtt ttttcttatt ttgcgggcga
     3841 aaaattccca ttgactatgg tgatgattct acaaacaatc gcagctattt ttttgggctt
     3901 tatctggccg ctcatccaaa ttgggattga taattttgca aaactgctcg ttgatatggg
     3961 ggcattaggt gtggggatat ttatgttttt gaatcgtttg ctgatcccct ttggactgca
     4021 ccatgtgttg aatacgtata tttattacga tttaggaagc tatacatctc ctagcaagga
     4081 agttttcaga ggagagatga cacgctttat caacggagat cctcaggctg gactgttttt
     4141 atcaggtttt tttgtagtaa tgatgtttgg tataccagct atatgtctag ctatctataa
     4201 agcagcttat aaagaaaata aagagatggt caaaggaatc atgggaagcg gtgcaggaac
     4261 ttcatttatt gcaaatatca ctgagcctgt tgaatttagt ttcatgtttc tttctcctat
     4321 gttgtacgtg atccatgcac tttttgccgg tttagcaggg attgtctgtt acttattcaa
     4381 tatacgaatt ggttttacat tcggagcatg tattgttgat tatctcatca attttcgaat
     4441 cgctaccaat gctattttga ttcttccaat cggaattttc ttttttgcct tgtattacgt
     4501 tactttttat tatttgatca ataagagaaa cattcagaca ttaggacgtg aagctaaggc
     4561 agagtttggc aatgaagtaa ccttagaaga aacagagtta gggttagcaa gtaaaaatta
     4621 tgaatatatg gcaacaaaaa tgctacaagc gtttggagga aaagccaata tcctggatgt
     4681 ctatagttgt aatacacgcc tacgggtaga ggtagtggat cccaccatgg tcgaggaaca
     4741 gcgaatcaag caattaggga taagcgggat cattaaacca actgaaaaaa attatcaaat
     4801 tatcatcggc ttagaagtga cttatgttat ggcagaattc aataaattat tagaagaata
     4861 gaaaggaaaa gatgatgatg aaaaaacaga ttatcactat agctggaggc gggagtacgt
     4921 atacaccagg aatcgttcaa gccattttga acaatgaaaa acggttgcca ttatctgaaa
     4981 tacgtttata tgatattgat gaagaaagaa atatggatat gtacttgatt gttaaattta
     5041 tgctgaaaaa aaaggttttt caaatattcg cattcgtgca acggatgatc caaagcttgc
     5101 ttttacagga tgcgattttg ttttctcaca aattcgtgtt ggaggattgg agatgcgaga
     5161 aaaggacgaa aagattccac ttaaacatgg attagtagga caggaaacgt gtggactcgg
     5221 aggatttgct tatgggatgc gctcaatcaa aggtttattg gaaattgtcg atcatataca
     5281 agactatgcc cccaaagcat ggattttgaa ttatacaaat ccagagtcga tcgtttctga
     5341 agctgttcgt cgtaaatatc cgaatgtaaa aatgattaat gcctgtgata tgactatttc
     5401 aattgaagaa acgattgcag tgaattacgg ttatgatcgt aagaattgga tcgttacgta
     5461 ttatggttta aaccactttg gctggtatac atcgatttat gataaagaat tgcaacgaga
     5521 aattatgcca gagattattg aaaaactaat aacaaaagaa atgcaagtcg ccgattttaa
     5581 tataggagac aaaacttggc aaaaaacctt tcaaatgatg tcagtcatca cgaaaaattt
     5641 tccaagtaat atcccgaata attacttaga gtattactta tatcctgata tggtcgttga
     5701 gcatactgac aaagagtata cacgtgctaa tatggtcatg gatggacgag agaaaaatac
     5761 caaagaaatg gctgataaaa tacgccgcgg tattgtggaa gaagtattga actttaattt
     5821 tggggagcat ggacaatata tcgtcgatat agctatttcc ttgttgaatg atgatcgtcg
     5881 tcgctttatg ctaatcgttc ctaatcaagg ggcgattccg aatctacggt cagatgcagt
     5941 tgttgaaata ccggcatatg ttggtgcaac tggtgtggag acaattacac taagaaaacc
     6001 aatcagtgat tttcataaag gattgatgga ggcacaagtt gcagcagaga agttactagt
     6061 agatgcttac tttgaaggtt cttatcaaaa agctttacaa gcatttacat tgaatcaaac
     6121 cgtaccaaat gccagagtcg caaaaaaaat actagatgaa atgatcaaag taaacaaaaa
     6181 atattggcca gcattaaagt agaggtgata aaaagtgcat ttctttaaaa caagaaacat
     6241 tctaaaagct gtagctagtg gtcaaattat tccattaaca gcagtaaaag acgaggtctt
     6301 ttcttcaaaa atgataggag ttggatacgc tataacgaat catgatggat atatccatgc
     6361 gccagttaaa ggaataatcg ataatatatt tcctacccga catgccataa cgatcgaaag
     6421 tgaatctggc agaaaaatac tgatccatat gggtttagat acagttgaac tcgaaggaag
     6481 tccgtttcag atacgggttg aaagtaacca ggaggtaaaa gaagggacac tgttagctat
     6541 tatagatttg aagaagttgg agcaagcaca aaaagatacg gtgatcattg tagtttctcc
     6601 aggcattttt gaaggaaatt tattaaaaca aaaccaagaa gtaaagatta aagatagttg
     6661 ttttgaattt tagtgtacga tgaa
//
LOCUS       DAKBVG010000141         6665 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000141 DAKBVG010000000
VERSION     DAKBVG010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.141"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            107..1450
                     /locus_tag="OUD09_002020"
     CDS             107..1450
                     /locus_tag="OUD09_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCT9693264.1"
                     /translation="MKKIVIIGSNHSGLAAARFLNLCDSNYEVILIDKKSEISYLECG
                     TSLFIKREVSNLEDLFYQNRRELNSKIKKLFLETAVDKIDIQNKKIICVKKDGTRFSQ
                     EYDKLILATGSKQIMLDVPGNNLANIFSIKNFPNAKEIFKALDDNTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGKKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHIDNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPVFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            1562..3013
                     /locus_tag="OUD09_002021"
     CDS             1562..3013
                     /locus_tag="OUD09_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCT9693265.1"
                     /translation="MKKSLKLASAVILMSQLFIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTLSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSAESSTTSSSTSEITSSKVTRPNHIESSKKEAPKTEGQTKNFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            3362..4033
                     /locus_tag="OUD09_002022"
     CDS             3362..4033
                     /locus_tag="OUD09_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HCT9693266.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRYLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCQCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEKWIAYITLK
                     KRGTI"
     gene            4581..6020
                     /locus_tag="OUD09_002023"
                     /pseudo
     CDS             4581..6020
                     /locus_tag="OUD09_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            6365..6532
                     /locus_tag="OUD09_002024"
     CDS             6365..6532
                     /locus_tag="OUD09_002024"
                     /inference="COORDINATES: protein motif:HMM:NF018933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCT9693267.1"
                     /translation="MGSECGHTDGKKSFTIREWTCPICRTHHDRNVNVSINILIKGYV
                     YKHCLRKHQEP"
BASE COUNT         2292 a          991 c         1324 g         2058 t
ORIGIN      
        1 aaagaggaag aatctttttc ttcctctttt tgttttttat tttgggagat aggttgttag
       61 ttaaaaatga agatactttt catagaaggg aaaagaatgt gattaagtga aaaaaatagt
      121 aattattgga tcaaaccatt cggggctagc tgcagctcga tttctaaact tatgtgattc
      181 aaattatgaa gttattctga tagataaaaa atcagaaatt agttacttag aatgtgggac
      241 atcgttgttc ataaaaagag aagttagcaa tttagaagat cttttttatc aaaatagaag
      301 agaactaaat tctaagataa aaaagttgtt tttggagaca gcagttgaca aaattgatat
      361 tcaaaataaa aagattattt gtgtaaaaaa agatggcaca agattttccc aagaatatga
      421 caaacttata ctagctacag gttcaaagca aattatgtta gatgtaccgg ggaataattt
      481 agctaatatt ttttcgataa agaattttcc aaatgctaaa gagattttta aagctttaga
      541 tgataatact attaaaaaag taggaattat tggagcagga tatattggag ttgaaattgc
      601 tgaagccata agaaaaagag ggaaagaagt atatcttttc gatgttgcag atagagtgtt
      661 gtcgacttat tacgaccgta gtttttcaga taaagttgaa gaaatactat caaaaaatgg
      721 aatacatctt tgtctatcag aaagtcctaa aaaatattta ggaagaaaaa agattgaaaa
      781 aatagtcact acaagtggta cccaatactc tatggatatg gtcgtacaag ctataggatt
      841 tctatctaat agtccaattg gcaaaaagga actcttgaga gatgttagtg gagcatatct
      901 aactaatgaa taccagcaaa caaatataaa agatatatat gctattgggg attgtgctac
      961 aacttttttt acgtccatta atagaagtag tgttgatttt tctatttcta atgctctaag
     1021 aacagcttat attgcaactc aacatattga caatcaagat tttacacctt ctggttccca
     1081 atttacaaat ggcttttcta tatttaatta caatttttac gcagcaggtt tgaatttaaa
     1141 aacagcacga ttattaagtg agtctataga ttatatagaa atagaagatt tcattagacc
     1201 tgtttttttg agaaaaaata gtcgtgtaaa attaagaatt agctttgaaa aaaatacaaa
     1261 gagaatagta ggtgtacaac tttgtagtca agaagattta tctggactat tatcaatgtt
     1321 ttctcttgct atagaagaac aaattacatt agacaaattg aaaatgttag attatttatt
     1381 tcatcctagt tttagccaac cctataattt tattttaaag gcagtaacag gattaaacat
     1441 tgaaacataa tcgttactaa atgaaatcaa gaaatatact gtattactca attcattgaa
     1501 aatatttatc ataattttta atagaaaaca ctattaaaga gaaaaaagag aggtaaatca
     1561 aatgaaaaaa tctttaaaac ttgcatcagc agttatatta atgtcccaac tctttatagc
     1621 aacacctttc catgtgttgg caagcgaaaa ttcaacatac aaaattgcaa aagaaagaac
     1681 cgagacgact gaaatgagta attccgatgt aacagcagag agtagtgaac ttttgacaac
     1741 tttatcaagt gaaagtagta cgacttcaaa tgctgagtct tcaacaactt catcaagtga
     1801 aagtagtacg acttcaaatg ccgagtcttc aacaacttcg tcaagtgaaa gtagtacgac
     1861 ttcaaatgcc gagtcttcaa caacttcgtc aagtgaaagt agtacgattt caagtgccga
     1921 atcttcaaca acttcaagta gtacaagtga aataacttct tcgaaagtga caagaccaaa
     1981 ccatatagag tcttcaaaaa aggaagcgcc aaaaacggaa ggacaaacaa aaaatttttc
     2041 gacaccacag caaatagaat caacagaatc tttccaatca caaccaatac tatctggtaa
     2101 tgaagaaatt cattttgaca aaaatgaatc tgtagaaaat tttattatga aaattggtga
     2161 gtctgcgaga aaaattggtc agaaatatga tttatatgct tctgttatga ttgcacaagc
     2221 gatcttagaa tctgccagtg gtcaaagcca gttagctcag gcaccgaatt acaatttatt
     2281 tgggataaaa ggaacttata atggaaactt tgtcattatg gtaacaaacg aagatttggg
     2341 taatggaacg ctttatacga cccaatcaaa atttagagtg tatgaaaact atgaggaaag
     2401 ttttgaagat tatgccaaat tattaactaa gggaattagt ggaaataaag atttttatgc
     2461 aggagcatta aaagctaata gtaaaacata tcgagaagca actaaattcc ttacaggacg
     2521 gtatgcaaca gatacacaat actatttgaa gctaaatgaa ttaattaaaa cctatgattt
     2581 aaccaactat gataaagaag ttaaaggtcc acaaatgagt aagactggat accaagtgcc
     2641 tattcataat tattcaataa ccagtttgtt tggaaatcgt ggaaatgaat ttcaccgagg
     2701 acttgatatg gcagctacac agggtgagcc gattcatgca gttaaagctg gtaaggttgt
     2761 taaagcagag tttcactact cttggggaaa ttacgtggtg atacaacacg aagatggatc
     2821 aaccgcgtta tatgctcacc agcaacaata cctagtaaaa actggagata atgttttgca
     2881 aggacaaata attggctatg taggaagtac tgggaatagt actggcccac accttcattt
     2941 ggaaatttgc aaagatagta gtctttctca gagcatgtta gttgatccga aaaccttatt
     3001 attcggagac taaaaaaggt aatcaatctg acaaagtaac aagaaaatgg ttggattctt
     3061 agacctttga tcaggatatt gagactggaa ttgatatctc gattacgatg agtttgacaa
     3121 ataaaacaag tccattatcg aatatcgagt gtattattgc cgtctttatg ctcatatttt
     3181 gagcaggttt tactagatga aaacaattta tctatcttga ggatttcgtg tacgtatcgt
     3241 tcagtcttgt attataaatt agtcataaaa tcagactaag aaaaattgaa aatatttttt
     3301 tctgatttat taaaagttaa ttgaaattat atggtacttc tttcttggga gggtaggtgc
     3361 agtgaaggaa agatatggtc accttactgt tgttgaggag attactcata atggatataa
     3421 aaaatatcgt tacctatgtg actgtgggaa tgaaacgatt gtatatcgat caaacttact
     3481 atcaggtaga accaaaagtt gtggatgtgg aaattataaa aataggcata aatataaaaa
     3541 tttgacaaat aaaacttttg gtaagttagt tgcaatcaag ccaactaaac tgcgaagtga
     3601 aggaactgtt gtttgggaat gtcaatgtct gtgtgggaga aaaaagttag tatcggcaaa
     3661 gcgacttcta aaaggtgatg taaaaagctg tggatgtttg aaatcaaaaa agcatcacat
     3721 tgaaaataaa aaattcggat tacttactgc aaagtatcct ttggacaata actgttcatg
     3781 gaaaacaaaa tgggtttgtc aatgtgagtg tggcagtatg tgtgagataa gttatagtaa
     3841 cttatttttt ggacatactc aaagttgtgg atgtttaaaa aggcaagaaa caaggacatg
     3901 tgttgaaggg acagtaatag agtgtctaac gtcaaaatta ccgaaaaata atacaagtgg
     3961 tgttaaggga gtttatcgtt ctaaagagaa atggattgcg tatataactt taaaaaaaag
     4021 aggtactatt taggatcgta tgattctatt aagagtgcag ctcaagttag aaaagatgct
     4081 gaaaataaat tgtttcaacc tttgattgac aaatatcgag gtaatcatta attattgtgg
     4141 ataataatta acagtgatat ataaacatct ataatatatt attcatacta ttgacattta
     4201 gtataatcct atggaattga atatcatagc aatgtatttg aaaagcaaat tggaaaacaa
     4261 ataggtgcaa caccataaat atgaaaaaat tgggaaaaag tagaatggaa attttcttac
     4321 gtctcacgag atagctcaca ctattaatct gaagaacaat gaagtcgact tttagaaatt
     4381 gctagaagaa agtaaagcga attatcacta gttactatag agtaagttca aataaagcat
     4441 ctcaaactta catggttaca tgagaataac aaatcgaaat tactcaagct tcctatggct
     4501 agagtcgcga gaagttgttg ggcagtagac ataggttata ttgttcttag ctacacgatc
     4561 attagaaagg aaatcctatt atgcatagtt atcaagaaga tgcaaaagtc caaaaaaatc
     4621 gctggtggat tttgattgct atatctatgt ttaccttcat gtccacattg gatggcagta
     4681 ttgtcaatat tgcactacct accatctcag atgatatggg cataccaatg aatcaagcgg
     4741 aatgggtagt gtccgtttat ctgatgattg tctgttcttg tttgttgctg tttggtaaaa
     4801 ttggcgatag ctgtgggaaa atcaaagtat ttcggattgg tacgctgatc tttgtcttgg
     4861 gctcgctgtt gtgtgggttc aatcattcgc taggtattta attgttcgca cgggtggtac
     4921 aagccattgg tgctagtatg actatggcta caaatgccgg aatcatcacg gaagtctttc
     4981 ccatacatga acgtggtcgg gcattgggag caatcggtgc gcttgtttca ttaggctcga
     5041 ttgctggacc aggtcttggc ggcttgattt tgtcccaatt ttcttgggcg tacatttttt
     5101 ggatcaatgt accggtggga atcgtaacga tgttgattgc tgaaaaattc ttaccaaaag
     5161 atatcaccat gagcggccat acaatcgatg gagggggctt tgctgccatt gcggctacga
     5221 ttatcagcct ttttggaaca atctttttgg ggcaagaaag tggctttttc catccagtcc
     5281 cccttgtact gttcgtaatt tcagtgattg cctttgtcag ctttttaaaa gttgaaaaga
     5341 aagcagcctc tccattgatt tctttcggta tttttaagaa caagattttt accatgagct
     5401 tgattacagc cttgttgatt ttttcttcca acttctttgt taatgtagtg attccttttt
     5461 atctgcaaaa tgctcgcggg ttgagtccta gctatgccgg actgttgatg atggtctttc
     5521 ccttgatgat ggtcgtcggg gcgcctttaa gcggatacct gaccgaccgg gttggcccgg
     5581 agatttttgt attagtaggc ttgggtttat tgtcggtaac ccaagtcatg tatatgtttt
     5641 tgtcagtgga ctctcctttg tggctgtaca ttgtcgctac tgccatcatg ggtgccggca
     5701 atgccttgtt ccagtcccca aataatacga tggttatgag ttctgtcact atcgaaaatt
     5761 tgggactggc aggctccatg aattcctttg cccgaaattt ggggatggtc atcgggattt
     5821 cgctttctac gacggtctta tatcaagcga tgtctgctaa gatgggccaa cgagtaacta
     5881 actatatcgc agatcggccg gatgtctttt tatatggaat gaagattacc tttttaggca
     5941 gctttgtttt gtgttttgtt gcctttttcc tgactatctg gcgtatcaaa aaacaaggag
     6001 gtattcagaa aaatgtctga aaaaccattg ttgaagaatt tttggaggcc tgcgtatggg
     6061 tgagtgggat ttccatggat gatggaagat gaagtgaact tctgaaaacc gaaaaactca
     6121 agtattatct tcaaaatact agtactcaat tagatgctcg atttgattac aagaagactg
     6181 cgaaagccac tggcaaccgc aagctaggta tccaccgatg gactacgttt tagaggcaac
     6241 tggttatgaa tacggagcta acgctctcat ctgcataaga atgcaacacc cagattatgt
     6301 aaagattttc gatacttctg tcaaaaaaat catcaagata gataaatgat ttccgtctag
     6361 tcaaattggt tcagaatgtg gacacacaga tggtaagaag tcattcacaa ttcgagaatg
     6421 gacttgtcct atttgccgta cacatcatga tcgaaacgtc aacgttagta tcaatatttt
     6481 gatcaagggc tatgtataca agcattgcct tagaaaacat caagaacctt aggaacaacg
     6541 gggatagatt ggttaataag agatatcgct gttagtaaag acataggctt gcaagtatac
     6601 tcttttatca agaattccgt ggctttagtc atgataggtt taatactatg aaaattttct
     6661 ttagc
//
LOCUS       DAKBVG010000142         6597 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000142 DAKBVG010000000
VERSION     DAKBVG010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.142"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            84..2681
                     /gene="adhE"
                     /locus_tag="OUD09_002025"
                     /gene_synonym="adhC"
     CDS             84..2681
                     /gene="adhE"
                     /locus_tag="OUD09_002025"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCT9693268.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            2910..4016
                     /locus_tag="OUD09_002026"
     CDS             2910..4016
                     /locus_tag="OUD09_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HCT9693269.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            4033..5057
                     /locus_tag="OUD09_002027"
                     /pseudo
     CDS             4033..5057
                     /locus_tag="OUD09_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            5063..5542
                     /locus_tag="OUD09_002028"
     CDS             5063..5542
                     /locus_tag="OUD09_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HCT9693270.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKARLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            5558..6136
                     /locus_tag="OUD09_002029"
     CDS             5558..6136
                     /locus_tag="OUD09_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HCT9693271.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGAFDVHPKS"
     gene            6168..>6597
                     /locus_tag="OUD09_002030"
     CDS             6168..>6597
                     /locus_tag="OUD09_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCT9693272.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT         2156 a         1141 c         1471 g         1829 t
ORIGIN      
        1 agtgtttaca tagaaaaata aatattgtat gatatacatg tgaattattt aacaaataac
       61 catgacccat taggaggata atgatggcta aagtaataga aaaagaaaaa acgaagacaa
      121 ttgacgtaca agcaatgatt gatgatttgg cagaaaaagc aaatgcagca ttgaaagaaa
      181 tggaaagctt tgatcaagaa aaagtagacc atatcgtgca tgaaatggca atggcagctt
      241 tggatcaaca tatgccatta gcgaagatgg ctgtagagga aacaggccgc ggtgtttacg
      301 aagataaagc aatcaaaaat atgtatgcgt ccgaatatat ttggcataat atcaaacatg
      361 ataaaacagt tggtgtgatc aatgaagatg tccaaaaagg gttgattgaa attgctgaac
      421 cagtcggtgt ggtttgcgga gtaactccaa caacgaatcc gacttccaca acgattttca
      481 aatcaatgat cgcgttgaaa acaagaaatc caatcgtttt tgctttccat ccaagtgccc
      541 aaaaatcttc tgcagaagca gcacgagtgg tacgtgatgc tgctatcgct gccggagctc
      601 ctgaaaattg tatccaatgg atcgaacatc cttcgatcga agcgacttct atgctaatga
      661 accatccagg tatcgcgatc gtcttagcga caggtggagc tggaatggtc aagtctgctt
      721 actctacagg aaaaccagct ttaggtgtag gtccaggtaa cgtaccagct tatattgaaa
      781 aaacagcaaa aattaaacgt gcagtgaacg atttgatcgt ttcgaaaaca tttgataatg
      841 gaatgatttg tgcttctgaa caagctgtga tcgtggataa agaaatctat gcagcagtaa
      901 aagcagaatt ccaagcacac caagtttata tcgtaaaacc agatgaattg caaaaattag
      961 aagatgcagt gatgaacgaa ggaaaatatg cggtcaatcc atcaatcgtt ggacattctg
     1021 cgatggaaat cgcaaaattg gcaggaatca gtgtaccaaa aggaacgaaa atgctgattg
     1081 ctgaattgga aggcgtaggc ccagattatc cgctatcaag agaaaaactt tctccagttc
     1141 ttgccatgat caaagctaac aacacggacc atgcgtttga tttgtgtgaa ggtatgttga
     1201 atcttggagg attaggacat acagcagtta tccattcaga aaacgaagag ttgcatgtca
     1261 aattcggtct tcgaatgaaa gcttgtcgta ttttagtgaa tacaccatct gcagaaggcg
     1321 gcatcggtga tatctataac gaaatgattc cgtcattgac tttgggatgc ggttcttatg
     1381 gtaagaactc tgtttctcgt aacgtctctg ctgtaaactt aatcaatgtc aaaactgtag
     1441 cgaaacggag aaataacatg caatggttca aattaccgcc aaaaatcttc tttgaaaaga
     1501 actctctatt gtacttagag aaaatggaaa acgttgaacg tgtcatgatc gtttgtgacc
     1561 ctggtatggt ccaattcggt tactgcgata cagttcgtga agtattggca cgacgtaaaa
     1621 atgatgtgaa gatcgaagtc ttctcagatg ttgaaccgaa cccatcgaca aataccgtat
     1681 acgcaggaac gaaactgatg gcagacttca agccagatac agtaatcgct cttggaggcg
     1741 gttctgcaat ggacgcagct aagggaatgt ggatgttcta tgaacaccca gacacatctt
     1801 tctttggtgc aaaacaaaaa ttcttggata tccgtaaacg gacatataag atcgacaaac
     1861 cagaaaaaac acaattcgta tgtatcccga caacatcagg gactggttct gaagtaacgc
     1921 catttgcggt catcactgac agtgaaacac atgtgaaata tccgttagca gattatgcat
     1981 tgacgccaga tgtcgcaatc gtcgatcctc aattcgtcat gtcagttcca gcatctgtca
     2041 cagcagatac cgggatggat gtgttaacac atgcaatcga atcctatgtt tcagtcatgg
     2101 cttcagatta tacacgtgga ttgagcctac aagcaatcaa gttagtcttt gactatttag
     2161 aaaaatcagt aaaaacacct gatatggaat cacgtgaaaa aatgcacaac gcttctacaa
     2221 tggctggtat ggcgtttgct aacgcattcc taggtatttg tcactcagtc gcacataaaa
     2281 tcggcgggga atacgggatc cctcatggac ggacaaatgc gattctttta ccgcatatta
     2341 tccgctataa tgcaaaagat ccacaaaaac atgcgatgtt ccctaaatat gactatttcc
     2401 gtgcagatac agattacgca gatatcgcaa aattcttagg tctgaaaggc gaaacaacag
     2461 aggaattagt cgaagcttta gcaacggctg tttatgaatt aggaaaatct gtcggcatca
     2521 acatgagtct aaaagctcaa ggagtcacac aagaaacact agatacaacc gtagaccgta
     2581 tggctgaatt agcttacgaa gaccaatgta cgacagctaa tccgaaagaa ccattgatct
     2641 ctgaattgaa acagatcatc attgatgctt acaacggata agcaaaagaa agcgtagaaa
     2701 aaagataaga aaatagatga aaaagaactt ggaaaacgtt gtttctccaa gttctttttt
     2761 taatttccaa gtaactgaaa gttacttttg ttatttaaca tttaggtcaa ttttagacag
     2821 tttttgttca aaaagcgata tttatagtgg acggtattac tgctatatta aggatataaa
     2881 atgaaagcgt ttaaaaaggt ggcgtaaaga tgattattaa agaagagatt tttgtaccta
     2941 aagaattagt gaaagaaaaa attgacctgc tcgttgaaaa cctgacaacg atcaaagacg
     3001 ataatggtga atttttgttg gactttgatg gactgaaagt agatgacaaa agctggacag
     3061 tgtggaattg gccacaaggt gtcggtctat atggtattta taaaaattac cagatgacaa
     3121 aaaatcctcg cgcttatcaa gtggtcaatg aatggtttga ggatcgtatg gaagaaggcg
     3181 ctccgcccaa aaacgtcaat acaatggcac cgctattgac tatggcttac ttgtatgaag
     3241 acacgaaaga ttcacgctat ttgccttatt tagaacaatg ggcagaatgg gtcatgaatg
     3301 acatgcctcg gacaaatgaa gacgggttac agcatgccac atatggaccg gaaaataaga
     3361 atcagttgtg ggacgataca ttgatgatga cagtgcttcc tttggcaaaa atcgggaaac
     3421 tgttgaatcg cccagaatat ttagaagaag ctcgttacca gtttatgatc catatcaaat
     3481 acttgatgga taaacgaaca ggattgtggt atcacggctg gacgtttgaa ggaaatcata
     3541 actatgccga agcattttgg gctcgtggaa actgctggtt gacgattgct attccagaga
     3601 tcattgaaat cttagaatta tcgaaagaag atgcattgcg caaattattg atcgaaacac
     3661 ttgaagcaca agtgcgtgca ttaaaaactt atcagtctga aagcggttta tggcatacgt
     3721 tgttggatga tccttcttct tacgtagaat cttctgcaac tgcaggattt gcttacggta
     3781 ttttgaaagc ggtccataaa cgttatttac cacaagaata taaagaagtc gcttatcgtg
     3841 ctatccaagg attgcttgaa caaatcgatg aaaaaggaga agttcaaaac gtttcaatcg
     3901 ggacaggaat gggggacagt ttagattttt atcggaatat cggaatcaca gcaatgcctt
     3961 atggacagtc gttaacggtg ctttgtttga cagaattgct cgtctcttat tgttaaaaga
     4021 aaggaaaccc atatgaaaaa aacatcactg ctgtttgcag caagttcgct tagcctaggt
     4081 ctgctaggtc taagcggctg cggaaataat tctagtgtgg caactgtcgc agatgcagac
     4141 gtaaatgaga tagttacctt gcgctttgct tatgcaagca acagccagta attgatgcaa
     4201 tgaatgaatt tgggcgttta gtaaaagaaa aaacagatgg ttccgtacag atccagtatt
     4261 tcccagatgg acaactgggt ggagaacgtg aactgatcga attgacacag tcaggagcag
     4321 tagatttcac aaaagtaagt gcctcagcgt tggaaagttt ctctaaagat tattcgattt
     4381 tttccgttcc ttatctattc aatgatgaag aacatttctt taaggtgatg gataatgaag
     4441 aaataatgga gccaatctac caatcgacca aaaatttagg atttaccgga ttgacctatt
     4501 atgattctgg tcagcgcagc ttttatatgg tagacggtcc gatccatacg ccggaagatt
     4561 tgaagggaaa gaagatccgt gtgatgcaaa gcgaaaccgc tatcaaaatg gtagagcttt
     4621 taggcggttc gcccgtacct atgggaagtg atgaagtgta cacgtctctt caatccaatt
     4681 tgatcaacgg atcagaaaac aatgaatttg tcttgtatac agctggacat ggcggggtag
     4741 ctaaatacta ttcctatgat gagcatacac gtgtgccaga tatcatcatc atgaatgatg
     4801 ccatcaaaga acagctaact gatgaacagg aaaaagccat tgaagaagca gcgaaagaat
     4861 caacggtttt tgaaattgaa gcttttgcaa aagcaattga agaagaaaaa gctttagcac
     4921 aagaagaaca tggagtccaa ttcaatgatg tagataatac accgtttcga gaggcagttg
     4981 ccccacttca tgatatattc aaaaacgatc ctacctacaa agacctttat caaaagatcc
     5041 aagagcaagg agcgtagctg taatgaaaat gattttaaac aaggtattag agatcctggg
     5101 agctgttttg cttgttggga tggtcgctgt tgttttatgg caagtatttt ccagaacggt
     5161 tttaggaaat ccaaatacgg tcacggaaga gcttgttcga ttcggtcttg tctggtttgc
     5221 catgttgtct tccgcgtatg ttgtgggaca aaaagcacgt ttagccgtaa cggttttaag
     5281 tgaaaaatta actggcaaaa aaagtaaaat tttagaagtt gttgttcaaa gtttatttct
     5341 ggtatttgct gcgatgatca tggtgtatgg cggatggaat gctgtcacat tgacaatggg
     5401 gcagatctcg ccttctttaa gtattcctat gggatatgtt tatctgtcag tacctgtatc
     5461 gggggtactc atcattatct atagcctgat caatctaatc gatacattaa gcaacaagcc
     5521 agcaattcaa gaaaaaatat aaaaatggag gaataaaatg gcaatagaag ccggattagt
     5581 gttatttttt gtatttgttg tgttgcttgt attagggatg ccgattgcta taagtattgc
     5641 attctcatcg atgatgactt tattgttagt tataccgttt gatgtttctg cttttagttc
     5701 agctcaaaaa atggtgggaa gtatcaatag tttttcgctg atcgctattc cgtttttcgt
     5761 tctctcgggc attatcatga ataatggagg gatagctaag aaactagtgg attttgcgat
     5821 gttgttcgtc gggcgcgtgc cgggggcttt ggctcatacg aatgtgttag ggaatgcttt
     5881 atttggttcc atctctagct cggccattgc cgcttctacc gcgattggtg gagtgttgat
     5941 ccctcagcaa gtggaagaag gatatgaccg gaagtttgcg acagcggtca atatcgcgtc
     6001 agctccgaca ggtatggtca ttcctccaag tactgctttt atcatgtatt cacttgttgc
     6061 tggtggtgct tcgatctctg ctttgtttat gggaggttat ctcgtaggcg cttttgatgt
     6121 gcaccccaaa agttagacta gaaatctaat taaaaggggt gctttttttg cggaaatata
     6181 catttgtttt taagaaaaaa gtagtttcag actatttaaa taacgaaggc ggctacaaat
     6241 atcttgcaca taaatatcaa ataaatcgta cactggttag acattgggta aggatttata
     6301 actatcatgg ttgggaaggc ttggttggag gtggcaaaag ctacactaca aaatttaaac
     6361 ttgatgttat agaatatatg gaaacaaatg gtctttctat ccaagaaact gctaaaaaat
     6421 ttaatatcgg ttcaaataga actctaagta aatggataga gcaatatgaa gaaggcggtg
     6481 cttcttcact tgagagccaa aaaaggggca gaaaaattag tatgaattcc aagctaaaca
     6541 ttcctaaaaa acttaaagat gagtctcttg aagaagaagt tattcgttta agagcag
//
LOCUS       DAKBVG010000143         6589 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000143 DAKBVG010000000
VERSION     DAKBVG010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6589
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.143"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            <1..699
                     /locus_tag="OUD09_002031"
     CDS             <1..699
                     /locus_tag="OUD09_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HCT9693273.1"
                     /translation="ILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKN
                     MSYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRL
                     YAGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQ
                     YIEEYISDILAVHKIHSETTAITEDQLSKYKLLEYDLIVTNQPILNAHVPHILIDDSV
                     SFANEEELIRLFEL"
     gene            complement(772..1419)
                     /locus_tag="OUD09_002032"
     CDS             complement(772..1419)
                     /locus_tag="OUD09_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HCT9693274.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(1427..2113)
                     /locus_tag="OUD09_002033"
     CDS             complement(1427..2113)
                     /locus_tag="OUD09_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT9693275.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(2119..3192)
                     /locus_tag="OUD09_002034"
     CDS             complement(2119..3192)
                     /locus_tag="OUD09_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT9693276.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            3414..4088
                     /locus_tag="OUD09_002035"
     CDS             3414..4088
                     /locus_tag="OUD09_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCT9693277.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            4093..5433
                     /locus_tag="OUD09_002036"
     CDS             4093..5433
                     /locus_tag="OUD09_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCT9693278.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(5582..6568)
                     /locus_tag="OUD09_002037"
     CDS             complement(5582..6568)
                     /locus_tag="OUD09_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HCT9693279.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         2016 a         1258 c         1147 g         2168 t
ORIGIN      
        1 atcttgaaaa atctcttagg aaaattgaag tacagcagct atataaaagt agtgaactat
       61 tttgtcaata cgattttttc agaattttcg gagaaagaaa aaaatgaaat cagtgagcag
      121 atacgaaaaa atatgtccta ctgttcgttt tcatccgctg attgcaaaga aaaaaacgaa
      181 gaatatgttg ttcaattatt aacgcaaatt tattttttct cccgtatttt cccatacgat
      241 acgactcatt tgacgatccg tgcaacttac tttgcacagc agatgcaaca ccgccagata
      301 cgtctatatg ctggatttcg tgcagaactt caattttatt ccactctact gaatcagaat
      361 ctgatttatt atttatctag ctatatcttt tggatgctta tcggtaatcc tgaaattcac
      421 cattatactt ctgataaaaa aatactgatt atcagcgact taagtcttcg acatagtcaa
      481 tatattgaag aatatatcag tgatattctt gctgtgcata aaattcactc tgagacaact
      541 gctattactg aagatcagct gtcgaaatac aaacttctcg agtatgatct gatcgttacg
      601 aatcaaccca ttttgaatgc ccatgttcct catatattga ttgatgatag tgtgtcattc
      661 gcaaatgaag aagaactcat ccgcttattc gagttatgaa taatcaaaaa acagctatgt
      721 ataaaccacc ctgataaaaa tttcaggaca aatacacata gctgtttctt tttattgtat
      781 tccttcagca atccaacgag atttatagcg aaagtctttt aacaactctt cttttgtcca
      841 attatttgca atgagatccg tcaaagtgtt cattacgagg gtcatggaaa agtataggaa
      901 actaactgct tcttccgacg attgaatgga gaaatgattg attcttaata attccagcca
      961 tttttcgttc attggagaat cagccgatag ttctctacgt ttggttgtct gctgatcatt
     1021 tcgctgggtt aacaagtata acctttgcca atagatactt tcgtgatcta agtcgcagca
     1081 ccactctaaa tatttttcga tccccaaaaa gaaatgttgt ttgttttcgc taatgaattt
     1141 taagatatct tgatggacta tgtgagaact gcttttgatc gtatacaaat aagcatcttc
     1201 caaatcttga aaatatttat aaaaagcacc gcgcgacatc cccatcttgt caacgatttc
     1261 tgagactttg acttggctga tgtgctgtga gcaaaatatc tccagtaata ggtcatcgat
     1321 tctctgtttc tttgcgttcg ataaattgaa aaaagtttct tttggcatcc acatctctcc
     1381 tttatctctg attatacgtg gatagccctt cattgtcatg aaggattcat tccttctttt
     1441 tctcttcttc tgtcaagtac ccatcttcca ttcgataaac gcgatcactc cattgaatca
     1501 tacggacatc atgagtaacc ataatagtcg ctttttgttt ctcatgagct tctttaacta
     1561 gtaatttgac gacttcatat gcatgatctg tatccaaact agctgttggt tcgtctgcta
     1621 agatcaagct tggctcatta tataaagcgc gagcaattgc cactctctgt ctttctccac
     1681 cagaaaggtc tttaggataa ctatttttaa gtgactggat atctaaagat tgcagcaagg
     1741 aatcgatttt tgttgaatct tctttttttg aagtaacttg gtcaactagt tcaaattgtt
     1801 cctttaccgt taaaaaagga atcaaattag agctttggag aataaatcct atttctttaa
     1861 agcgtaaatc tgctcgtttt ttttctggaa gctcagttag tgagtgaccg ttgatcacta
     1921 tttctccgga agtcggtgtt tgcaatcctc ctgaaatggt aaggaaagta cttttacctg
     1981 aaccagaagg accaatgatg ctgacgaatt ctccttcctt tacataaaaa tcaattgctt
     2041 tcaaagctga gacctttgta tgaccagaac caaatgtttt ggtaactttt ttcatcgtta
     2101 atattttttt catttttatt aacctcctat tgctgtaatt gggtctactt tggttatcgt
     2161 acgtatcgaa aacaatccgc ctagcattgc gacaatgatc aaaatcccac tatatagcaa
     2221 ccattgtgac caaaaaacag caaaaggcat cgcgcttggt aaaatcagac tagtcaaata
     2281 agcaaacaaa agagcaagca gaacgcctac caccccaaca ataaaggact gtgctactaa
     2341 agatttagcg atgaagaaat ttttgatccc ttgtgctttc attactccaa aaatagcagt
     2401 cttctgaagt gtcatcacgt acatgaaaat accgaccacg gcagcaacca ctaagaacaa
     2461 aaagtatatc atcgcattta aggtcatatt ttgggcagaa tagccaggaa tactttcaat
     2521 gaatgtccca atagttaata actgtagacc cttcgatgct ttttctttcg aaattttagc
     2581 tgaatccttt attacgatcc cattgatcgg ctgttcttct tcagaagcaa acggctgttg
     2641 tccatacttc aaagcagtcc acgtatctag ggacgcatag atgacgggag aaactgtgta
     2701 ataagtttca ggaaagatac cgacaatcgt caaaggatcg tcgtaactgc caattttgat
     2761 cgtatctcca attttatagc ctgaatcagc cagattttga gagatgatga tttcattctt
     2821 tgttttaaat tcatgccctt tcgtgagttt tggtaataaa aatgcttctt tcgttgtgcc
     2881 gaaaactgta acattttctt tgttttttcc tttgattgct ccgctgtaca atccgattgg
     2941 tgctttctct cctccttcaa tgtatttcag atctcctctt gttaattgtg aagctgcaaa
     3001 tacttgattt gcatcctccg acaaaacaat ctcttgtgcg tcccaatcat cgattgcttt
     3061 tttaaactct tctgaaagtc cattcgctag tcctgacaac atgaatacga catacgcaat
     3121 caagaacata ataccgacga tcaagccata tcgtaattta gaatagcgaa tttctttgat
     3181 agctagaaac atttgttttt cctccctatg atagattgat agtgacaaaa tgtcacttgt
     3241 tgaatagtgt aactctaaag tgacaatttg tcacttgtgt tgtttttttc ttatcaataa
     3301 attgtctaag acatctttcg ataaaacctt aacatgagtc ataaaaaatt tatttaagcc
     3361 ttatttatgc tttccatgat aaactaaagt gaccaaatca atgaggtggg aaaatgatta
     3421 aactattagt agtagaagac gataaagtat tatccaataa tataaaagaa atactcgctg
     3481 caatcggcga gataacacaa gtttatgatg gtagtgaagg actgttcgag ttggaaagtg
     3541 gagtttatga tcttgcaatc ttggatttga tgatgccgga aatgaatgga tatgaagtgt
     3601 tagcaaaaat acggaaagaa aaaatacaga ctccagtatt gattttgact gcaaaagata
     3661 gtttggaaga taagattgaa ggatttgaac gaggtgcaga tgattatcta acaaaacctt
     3721 tctatcgaga agaactgatc atgcgcgtga aagcattatt gaaacgctcg ctaggtctct
     3781 ttgatgaaaa tgttctgaag tataaggata tcagttgtga tttaaaaagc aatgaagtct
     3841 tgtataaaga acaagttttg cccatacaag gaaaagagtt tgaattgctc gtttattttt
     3901 tacaaaataa gggaatcatt ttgacaaaag agcagatatt tgaccggatc tggggatttg
     3961 attctgatac gacgattaca gttattgaag tttatatgag caacttacga aaacatctgc
     4021 gaagtactgg attggataaa gaaatcaaaa ccttacgtaa tgtcggttat atactgcaaa
     4081 gggtgtgatg caatgaatgg agatattaca cgaaagcaac gtagtcgctt ttttgcgaca
     4141 aatgtagcag catttgcttt tatctttttt cttttgggat tcattacttt acagatatta
     4201 aaccaatctg catacagaga aacagatctg tcattagaga aaataaattt tgattctcga
     4261 acagtacaat tagagataca aagatatcag caaggcgatc cctttttaaa cccgactcaa
     4321 acggaacaaa acagaacaaa catggaacag ccattaatgg aaccatcaaa tgaccgtttt
     4381 aacactcaag tgatcttgtg gtctaaaaat ggtgagatat tgaataaagc agctattggt
     4441 gggcgtttca accaaatcaa agaattaaaa atggatacga aaaatctgaa taccatccaa
     4501 aatattgaat taaatgaaga cagtggctca tctgccctct ctttccgttc gattacgaaa
     4561 aaagcggaga cgaatcaaga tggcgttgcc tacatccaaa ttttagaaaa tacaaatcaa
     4621 atcaagaatt cattagcgac ttttcgcacg attatgattt tgtgtatgat tgttttttgg
     4681 attctctcta ttggtatcag ttattacttg tctagtctaa gcatgcgccc tattattctt
     4741 tcttggagaa gacaaaaaga atttgttgag aatgcttctc atgaattgcg cacaccgtta
     4801 acgattattc aaaatagttt ggaacatctt tttaccaagc ctaatcatac tattattgaa
     4861 gaatccgaaa gcatcgctca agcattgtca gagacgaggc gcctcactgg attgacttcg
     4921 gatttgctga caatcgctcg gaatgattct gatcagcagc tgctttcaaa gcaaatgatc
     4981 aatacacaag aatatatcga acaacttgtt aaaccatttc aagagatggc catcatagat
     5041 ggaaaagagt ttattctcca taacgatgca acattagaag taccaattga tgaaaaaaaa
     5101 atccatcaag ttttggttat tttgctggat aatgcattaa aatatacgaa tccagaagat
     5161 aagatcattt tggattcatc tgttaaccat aaagaatggc tgattcacgt aaagaatact
     5221 ggcccttcta tcagtgaaga agatcaagca aggatttttg atcgatttta tcgggaagat
     5281 caatctcggg cgaaagagac tggcggatac gggcttggct tggcaattgc aaaacaggta
     5341 atcgaacagc atcatggaca gatttctgta tccaattatc ttccacaagg agtcgatttt
     5401 aaagtccaat tgccgttgaa gcctaaaaaa taatgcttat ccctaaaaaa gggctgtgac
     5461 aaaaatcttg tcacagccct tttttgttgc gaataaacgg tgttccagaa gtagcttctt
     5521 cggaaataag ccgaaatttc tttaagctga ccacttctgt cacaacctct ttagcttacg
     5581 cttacgattt tttattgcgg tcttcaatgt gtacatcacc gcttgtgtca atatccaatt
     5641 cttctcggct aacatcttct gtcacttttt caacatcttc atgttcttct ttatgaatcc
     5701 caacttcttc acgaataact gtatgtttgc ttacatctac ttgctcttct ttgattggga
     5761 tagtaatcgt gtcatcttcc aatttttcgt ctgttccctg tgaagattga tctgttactg
     5821 gtttgcgttc gatgacgatt tcttcacgtt tgaccggtac ttctaccgtt tcagtatcgt
     5881 tgaccacatg tttgtggatg tcgacttctc cagttgtcac atcatgtgta tttacatcta
     5941 atttttcttc ttttagttga attgtatcat catctagatt tggtttgcga tcgatgtctt
     6001 cagtcttgct tgtacccatt gtatcagtct cgccaggtac gtccatcgga tcagttcctg
     6061 gtgtcatatt caatggatcc gttgtatttt gaaagcctgt cgtgccacct acgtccattg
     6121 gatctgttcc agtgccagtt tctaatccag tccccattgt gcttgctggg ctaacagtat
     6181 cttgtgtatc cattgtatct tctgttgttt ctggctgacg atagcctttt actgtgatga
     6241 caaattttcc atcagaaata tctcctctat attgatataa aggatcattt tctggtgtag
     6301 ccgtctcaga atcataggta tcaaaagaaa aagtatcttt tattttttcc cataaagaac
     6361 ggtcttcttc accatccatc cgattttcat ctgtttcaac atctatacca gacaatccgt
     6421 caaatcctag tctagatgct gcttcatcag tagtataaat aacgatatca tctcgttcat
     6481 acccttcgtt acgcaaccgt tcaattactg ataatgcttc ttccctcgta ctatagcttc
     6541 cgatgaccgt taaatttttc tcttccatgt aaattcctcc tagcaaata
//
LOCUS       DAKBVG010000144         6308 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000144 DAKBVG010000000
VERSION     DAKBVG010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.144"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(15..365)
                     /locus_tag="OUD09_002038"
     CDS             complement(15..365)
                     /locus_tag="OUD09_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCT9693280.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(380..2107)
                     /locus_tag="OUD09_002039"
     CDS             complement(380..2107)
                     /locus_tag="OUD09_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HCT9693281.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEEAKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            complement(2321..3247)
                     /gene="trxB"
                     /locus_tag="OUD09_002040"
     CDS             complement(2321..3247)
                     /gene="trxB"
                     /locus_tag="OUD09_002040"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCT9693282.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(3392..4825)
                     /locus_tag="OUD09_002041"
     CDS             complement(3392..4825)
                     /locus_tag="OUD09_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCT9693283.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDVFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQVKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            complement(4825..6228)
                     /locus_tag="OUD09_002042"
     CDS             complement(4825..6228)
                     /locus_tag="OUD09_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCT9693284.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALNDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
BASE COUNT         1656 a         1472 c         1087 g         2093 t
ORIGIN      
        1 atttgcacct atcgttattg atgttccaca tgtttgagtg tctggttcaa aatatccagt
       61 acatgagaat catcaagact atacaaaatc gcttttccct cgcgatgaga cttgacgaca
      121 tgattttctc ttaataaacg tagttgatgc gatacagcag actgctccat ttccactgct
      181 tgggtgattg ctgtcacatt ttgttccccg tctttcagat atagcagaat tttaagacga
      241 gtgggatcac tcaacatttt gaataattga ctgactttct ctacttttga tttttgcgtg
      301 tctggcaatc cattcacctc caacttgaaa aaagactgac ttctaaagtt taccactttt
      361 tccatcagtc tcaagttttt tattcttctg tcatcttttt gataatcgct tcataagagg
      421 ctactttttc ggctgcgtct tctgtagagc ttccttttac accaatataa aacttgattt
      481 tcggctcagt tcctgaaggg cggatagcaa tccagccatt ttcttccaat gtatatttca
      541 atacgtcgga tggcggtgtt gttagttttt ctacgtttcc ttcacggtct gttctcgtca
      601 attctttaaa gtcttctgtt tgaataactt ttgctccggc aaattctgtc ggtgcttctt
      661 tacggaattt ttccatcaaa gcagagattt tcgcagaacc ttcttgtccg ctcattgtca
      721 cagaaatcgt tttttctgca aaatagccat aggtttcaaa aatcgtttgc aagccgtcat
      781 acaacgtttt gccttgtttt ttatagtaag ctgctacttc tgctaataaa agcaatgctt
      841 ggatcgcgtc tttatcgcga acaaaaggtt taactaaata accataactt tcttcgaatc
      901 caaacataaa agtatgtgaa tggtcttctt cgaattgttg gattttttca gcaatgaatt
      961 taaaccctgt caacacattg aacatggtag caccgtagtg ttccgcaatt gctgtaggca
     1021 attcgctgga aacgattgat ttcaataccg ctgcgttttc tggtaaagtg cctcttgatt
     1081 gatgtgcttc caaaatatat tgagcaaaca atgccccgat ttggttccca gtcaatactt
     1141 cataagaacc atctggcaaa cgaaccgctg cgcctaaacg gtctgcatca ggatctgttg
     1201 caatcagcaa gtcagctcct tctttttctc caagtcgtat agcatattca aaagctgaat
     1261 gttcttctgg atttggcgat ttgaccgtag tgaaatctgg atctgccaca gcttgttcgg
     1321 gaactagtac gaattgttcg aatccagctt gtttcaacgc acgttcgccc aacattttcc
     1381 ctgttccatg aagtggcgtg tacacaagtt tcaaatcttt ccccattgtt tctaccaatt
     1441 cacgatcaat cgtcactgct ttgatttctt ctagataagc agcgtccact tcttctccga
     1501 tgatcgtgat caacccgcta tgtttagctt cttcgtcaga taatacttcg acttttagcg
     1561 gattgctgac ttcacggaca tatttcgtca atgcatctgc gtctgcagga ggcatttgtc
     1621 cgccatcttc tccgtagact ttatatccat tgtaagccgc tgggttgtga gatgcagtta
     1681 ccatgattcc cgcaaacgtc ttcaaatgac gaactgcaaa agaaagctct ggtgttggac
     1741 gcagactttc gaatacataa gacgggatat tgtgatgtgc cagtgttttg gctgcttcca
     1801 ttgcaaattc tggtgactga tggcgtgagt cataagcaat agcgacacct cggcgttttg
     1861 cttcttcacc ttgtgtatcc atgaaacgtg ctagtccttc tgttgcttgt cttaccgtat
     1921 agatgttcat tcgattgatc ccaggtccta agattccccg catgcctgca gtaccgaatt
     1981 ccaacggtgc ataaaaagca tcttcaagtt tttcaggatc ttcacccaga tcatttaatt
     2041 gtttttttaa gttttcttct aaattttctt catgtaccca ttgttcatac acttgttccc
     2101 aagacatgaa aataacacct ctttctgaaa tatggttctt aataagtata gcattcactt
     2161 attagatatg aaagtatgag gaccaaaaaa cgcttaaatc aagaaaggat tcttttatca
     2221 aaaggtgttg gacgtgttcg cataaatcag attcagtaaa tttcacgcga aaaaaaagag
     2281 taaaagaaca attgaacccg ttcttctact ctttttaaac ttatgcagtt gtttcttcta
     2341 aagattccaa gtatttatac acttgttgtc cagcaatacc gccgtcacca acagctgtag
     2401 tgatttgtcg caaatctttt tcacgaacat ctccgatggc aaaaacgcct ggcatattcg
     2461 ttttcatttc gttgtctgtt ggaatccagc cagcttcgtt cgtcaaacct gcttctctaa
     2521 aaggtgcagt taatggatcc agtccaacat agatgaatac tccgtctgct gctaaatcaa
     2581 attcattgcc ggttttgaca ttacgtgctt tcacacctgt cactaccatt tcgtttccaa
     2641 cgatttcgtc aacgactgta tcccacataa aggaaatttt ttcatttgca aatgcacggt
     2701 cttggatgat cttttgtgca cgtaattcat ctctacggtg gacgatcact acttcagaag
     2761 caaattgtgt caaatagatg gcttcttcta ctgctgagtc cccacccccg atgactacta
     2821 gacgtttgtt acggaaaaac gcaccatcac agactgcaca gtaagaaacg cctcgtccag
     2881 caaattcttc ctcgccaggt acgcctaatt tacggtggat acagcctgtt gcgataatga
     2941 ctgtttttcc ttcgtaactt ttatcttcac agatgacttc tttatggctg ccgtgatcct
     3001 tgatatcttg aacgattcca taagcatttt ctgtcccaaa tcttgagaca ctttcataca
     3061 tcttataagc tagatcgggg cccatgatcg aatcgaatcc aggataattt tcgatttctg
     3121 ccgtattgtt catttggccg ccaggggcac cttgttcgat catcaatacg gataagtttg
     3181 aacgtgaagc atataaagca gcagtcatcc cagcaggacc tgcgccaata acgattacat
     3241 catacatatg tagattctcc tttacttaat caagtaagca tactttacag ccaatgcgaa
     3301 aaaaagtcta ttgttctgct tgcgtttctc gaatagcttt attatactcg aaaaatccgc
     3361 tgaaaaacaa tagactttta taaatcattg atcaatcgtt cgaaagattt tctccatttg
     3421 aatcaattac ttttttatac cagttgaatg attttttctt tgaacgggct aatgtgccat
     3481 ttccttggtt atcacgatcg acataaatga atccgtatct tttcttcatc tcaccggtac
     3541 cagctgatac taaatcaata catccccaag tcgtatagcc taatagatcg actccgtcta
     3601 gttctactgc atccttcatc gcctggatat gcgctgccag gtaatcgatt cggtaatcat
     3661 cttccacata accattctca tctggcgtat cgactgcacc taagccgttt tctacaataa
     3721 ataatggttt ttgataacga tcataaatat cattcatcgt tattctcaag cctaatggat
     3781 caatttgcca cccccattca cttgcttgca gatatggatt tttgacagaa gcaaagatat
     3841 ttcctgcagt ctgttccaag agttcgttgt cggtagtcga aactcgggaa gaataataag
     3901 aaaaagagat aaaatcaaca gtatgttctt tcagcaatgc ggcatcccct ggttccattt
     3961 cgatctgaat gccatttcgt tccatttctt tcaatgcata agctgggtat tctcctcgtg
     4021 actgaacatc aatgaagaaa taattttctc gatctgattt tctagaagcc caaacatctt
     4081 caggattgca agtgtatgga taatatgctc ctgctgccag catacagcct actttgttct
     4141 ccggatctac ttcatgagca atcttcgttg cgatggcact tgcaaccaac tcatgatgtg
     4201 ccgcttgata tttgacttgt tcttcgtttt ctccttcttc aaagtacagt cctgctccca
     4261 tgaatggtgc atgaaggatc atattgatct cattgaatgt cagccagtat ttgacaagtc
     4321 ctttatatcg attgaagatg actcgacaca gattttcata aaaaccaacg agttctctgg
     4381 aacgccacgc accgtattct ttgatcaaat gcattggaca atcaaaatgc gtaatcgtca
     4441 caagtggctc gattccgtgc ttttggcatt ctttaaagac atcctcataa aatttcaagc
     4501 cagcttcatt cggctctttt tcatccccca ttggaaaaat acgactccag gcgatggaca
     4561 ttcgataagt cttgaacccc atttctgcaa acaaagcaat atcttcttta taacgatgat
     4621 acatatcgat tgcaattttt gctggataaa aatgatcctc atcaaattca aacatttttt
     4681 tctttccggt gataacagga aaacggtctt ttccgattgg cacaacatct acgtttgcta
     4741 gtccgcgtcc accttcatca taaccgccct cacattgatt ggctgctgta gcaccgcccc
     4801 ataaaaaatc ttttctgaat cccattatac ttcctccatt atatgtgtat tacctgataa
     4861 ttctcttgcc agttcatctt gaacttcgat tccttcttct tttaatcggc tgattgtttc
     4921 cttgaattgc ggtaaatact gctggtgtgc aataaacaac tcatccatga tccgttttgc
     4981 tgtctttcct gatcgtataa gcggattgat cgtaaacgcc tgtaaagctg tcccataatc
     5041 accagtcaca gctgcttcga tcgtcagcaa ttccatattc ttcattactt gcaaccagcc
     5101 tctttcagca gcaggaagag aaccaaacgc tacatttctc gctccttgtg cgccaacata
     5161 ggcagtgact tctactacac aatctgccgg caggtcagga acagctccat cattctttgt
     5221 agataccacg atctgtgttt ctttatttcc atagattgaa gcaatcgttt cacaggcagc
     5281 gtctgaataa taagcgccac ctcgtttttg caattgttca ggtttatggt ctaattctgg
     5341 atctttgtat aattcaaata gctctgcttc tgtttctttc acttgctgcg ctcttgtacc
     5401 aatcgtttga tattcttcta aagcatgttc cagcatttct tcttcacggt agtaatagcg
     5461 gtggtagcca caagggatca tattcatttg ttttaattgt tctttgaaaa acgggatatc
     5521 aaagatattt ttcggcaatc cattgtcttc atcgtataaa cgatcaatca attctgccgt
     5581 gcggtcattt cctttattat ctgctacttt gtgccaatgg aaatgattta gaccggcaaa
     5641 cttgtagatc agctcttctt ttttcttgcc aagcattgct ggttctgtca tcatcgccat
     5701 tactggcaca ttgcataatc caatcacttt atcccatttg ccatatctga taactgcttc
     5761 tgtcaccatc ccacttgggt tcgcgaaatt gatcagccaa gcatttggac ataaacgctt
     5821 catgtcttca acgattgcca agaccactgg aacggtacga aaagctttga atatcccgcc
     5881 agcaccattt gtttcttgcc caatcatccc ataataacta gggatccgct cgtctttgat
     5941 tcgtgcatct aattgaccga cacgaaactg agtcgtaaca aaatccgcat cattcaatgc
     6001 ttcttcccga tccagagttg catagatttt gacttcatac ggagaagcat cccacatacg
     6061 ctgagacatc tgactaacga tctcaagctt ttcttttcct tcttctacat ctacgagcca
     6121 gatttctcgt atcggtaatt cctcatatct tttgataaat ccttccatca actcaggggt
     6181 gtaactgctg cctccaccaa tcgtagcgat tttgattcct tttgccatgt tctcactcct
     6241 ttactttttt acactttcat tatctttttt gtaaacaaaa caaacaatag aaatccgttt
     6301 ctttccag
//
LOCUS       DAKBVG010000145         6303 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000145 DAKBVG010000000
VERSION     DAKBVG010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.145"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            334..714
                     /locus_tag="OUD09_002043"
     CDS             334..714
                     /locus_tag="OUD09_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HCT9693285.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPD
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            696..1610
                     /locus_tag="OUD09_002044"
     CDS             696..1610
                     /locus_tag="OUD09_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HCT9693286.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     TLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKK
                     KVRSNKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTP
                     EQSEIRPTRRNHEQHVERGTDHQQELSL"
     gene            1855..2544
                     /locus_tag="OUD09_002045"
     CDS             1855..2544
                     /locus_tag="OUD09_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693287.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLV
                     GVIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTA
                     CYEDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQT
                     EEACCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLES
                     VKEWLDQQQQN"
     gene            complement(2665..2751)
                     /locus_tag="OUD09_002046"
     CDS             complement(2665..2751)
                     /locus_tag="OUD09_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HCT9693288.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
     gene            3427..4377
                     /locus_tag="OUD09_002047"
     CDS             3427..4377
                     /locus_tag="OUD09_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HCT9693289.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     LTFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIR
                     GTAKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVV
                     GYQFTFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELE
                     LLQQIKQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
     gene            4398..4931
                     /locus_tag="OUD09_002048"
     CDS             4398..4931
                     /locus_tag="OUD09_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HCT9693290.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLRE
                     QLALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATE
                     TIDKQIDTEKKWWQLWK"
     gene            4947..5117
                     /locus_tag="OUD09_002049"
     CDS             4947..5117
                     /locus_tag="OUD09_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693291.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNS
                     PNEELFVKETDK"
     gene            5567..6100
                     /locus_tag="OUD09_002050"
     CDS             5567..6100
                     /locus_tag="OUD09_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693292.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENV
                     VIEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAIN
                     AAFWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQ
                     RGYDAAGRVVATRYSTL"
BASE COUNT         2322 a         1100 c         1176 g         1705 t
ORIGIN      
        1 aaaaaaaaat aaatcgatac attttagaag aatagggttg tttgaatatt acaaagcaac
       61 aatttcacct tcggtgaaat aagcgaaact ttacaaaaag tggctagcaa atgctagcct
      121 ttttttgtat tgtggagcgt ggattttgag ggggattcaa gggggagctt gcgctccttt
      181 gacaagcggt tatttgcacc aaatacggag gtatttggct agcaaatata gagcttgcca
      241 agccctttta gtgagcttta ctcattgtac ctttttaggt acaatggtat tgcgaacgat
      301 acgtccgtag gacgtactga aagggtggga cttatgagcg aacaacatca aaatttggct
      361 agcgatcatt ctaaaaaata tacctatcga tccgagccaa aacaaatcag ttttcgagtg
      421 agcgaatccg aatttgcaaa gctgaagcag tcagctgaag ctttgcaaat gagtgtgccg
      481 gctttcgtga aagccaaggc acaaaacgcg cgtctggtga caccgaaagt ggcgccagac
      541 attgcccagg caatcgcgcg cgatttagcc aaagctggag ggaatatcaa tcaaattgcc
      601 aagtggtgca atacacacca acacgacgtt gctcctgatg atgctcaacg tttatccgaa
      661 aacctaaaaa tcatgcaaaa ggagctacaa aagatatggc aacagttaaa gtaagtcgaa
      721 ccacttcatg tagccgagca atcaattacg cagaaccacg agcaaccgtc aaaacaggaa
      781 taaactgtga cattgattat gccaaaagtg aaatgaaaca aatacgcacg ctttatggaa
      841 aagacgatcg tgtacaagca cacttattga ttcagtcctt tcgaccagga gaaattaccg
      901 ctgaaaaagc caatcaacta ggggcagagt atgcgaaaaa aatagcccct aaccaccaaa
      961 tcgcgattta cacacataca gacaaagacc atattcataa tcacattgtc attaactccg
     1021 ttaatcttga aactgggaaa aaatttcaag cccatgggca agcattttta aacaaatgtt
     1081 atgacctaaa tgacgagatt tgtcttgaac atgacttaac aatcacggaa cgaggaaaga
     1141 aaaccgaaaa acgaaccatg tctgaaatta aattaaaaga aaaaaatgaa ccggtttgga
     1201 aagacgaaat tcggtttgcg attgatcaaa caatgaaaaa caaaaaaact cgaacgtata
     1261 cccaattttg tgattcactt aaaacattcg gcattcattg cttgaatcgc ggaaaaaatt
     1321 ttacttacga attggtccac aaaaagaaaa aagtacgatc caacaaacta ggaaaggatt
     1381 acgaaaagga gacgatttta catgagctgg acagacgaga acgaacctac aatcaacaac
     1441 gaaccaacga gctacgaaga gctcgagcta gcattcgcga aactgcagaa gaagtacgaa
     1501 gagaacaacg aacttttacg cctgaacaat cggaaattag accgactcga agaaaccatg
     1561 aacaacacgt tgaacgagga acagaccacc aacaagaatt atcgctctaa aacgttaaaa
     1621 gacatcgaaa acgaaataaa gagacaacgt gcctttcttg cgccttatac ccaagaaagg
     1681 atacaaacta cgatggattc acaagtcaaa aaactccacc aagcgctatt agaccagcga
     1741 aaccaagaac aaaaaaaggc tttaattcaa cgatcgatgg atattcttgc ccccttatgt
     1801 tgcttagtac taacattatt agccttttgg ttggtatggg gagaataaac agacatggca
     1861 ttctatttac cctatctact catttctgcc agtattttag gaattatttg gcttagctat
     1921 aaggcttatc aaacaagata ttcattaaaa ggctctaaaa ttcgatttaa gcgattttta
     1981 ttggtcgggg ttatatttgc cttatcaatc gttttaagtg gtttattcgg cgttttagag
     2041 ggtaataaac gtgtttcaag aagcatttta ttaggggata tcactcaaaa gtatgaagca
     2101 gcatgtgata aaaagaaaaa agagcaagca ttagcacaaa aaatggagga atttaccgct
     2161 tgttatgagg acatgaacaa tatttttgtg gatcaagaga aacgtttaac agataaaaac
     2221 atgaaaaaac tcactcgttt ttatcaaaac ttaccagaaa aacagcaaaa agaggtacaa
     2281 gaaaaatatg agcaagtaaa aaaagacgtg caatatttta aagatacgca gacagaagaa
     2341 gcttgttgta acttatttag cgatacaatc cctttttcaa ccagtgaaca agaacgaaaa
     2401 gaacggcaac aatcggtcac atacgaacgt tatgaaacac tgcttcagca agcaacaaac
     2461 atacaaaatc caacgaaaaa agaaacggca ttaaattatc tagaaagtgt caaggaatgg
     2521 ttggatcagc aacaacaaaa ttgaatttga ttagcaatca tgttaaactg aacctgcaaa
     2581 tgatcaaaca ttagatacac gaaagcccca gagaggtcgg attctctggg gctttttttc
     2641 aggttgatgt cgcctttatt gattttactt cttgtttcgg ttattcaacc aataactgaa
     2701 taaagagaca caaacttcta caagaagggg gcaaatgaac cgttcaaaca ttagatacac
     2761 ctccttccag tcgcaagact gtcggatagg cgacaagtta ttatacctta caaaataaaa
     2821 tctgtgtggt gtttttaagt atcccttatt ttcgggttgt tagggcttgc cctgaccgcc
     2881 tgtaaggcgc tttaagaaca agataaggtt ttttacccaa gactgtctta aaacgactta
     2941 gacgagctat cagaggcttc ccccacctcc gttactgact tgtttttagc aaaggagcct
     3001 ttaggcgact gcgcagtaca atgtgagagc atagcgatca cactttttta gcgaccgtag
     3061 ggagcaaggt tttgaattgg cagttttgaa aaatgcgcga acctgttggt agaaaaaaga
     3121 agtggttttt aggcgggaaa ttttgtcacc ctcttttata atctcttttt aaaccttttt
     3181 ttaaacctct ttaccgtcgg ctgtgtcctt actccttcaa tggatacagc gttttataaa
     3241 ctacattttg tctgctttta aactacattt tgtctgcttt taaactacat tttgtctgct
     3301 tttaaactac attttgtctg cttttaaact ctttttttgc actcacgtat gttataatat
     3361 gcattatcac agacaaaacg ttgtttataa cgtctgtatc cattgaagga gtaaggagga
     3421 atatctttgg caaatgaaat cgtgaaatac catcatgaat taaataccat tccgttgcgg
     3481 aaatttacac cagttgaaat gaatctattt ttctctattg tgtctagaat gcgcgaaaaa
     3541 ggcgataaaa cagtccgtct tacttttgac caactaaaag atttaagtaa ttataaagca
     3601 actgctaata atcgtttcat taatgattta gaaatgactt atgataaatt aatggattta
     3661 cgttttggta aaagaagtgc taatgggctc gatatagata gattcgttat gtttagtgag
     3721 tttaaaatac gtggaactgc taaagatcca tatgtagaca tacaagttta cgaaaaagct
     3781 ttacctttac taaacaattt agatgagtgg gttcgctatt cactccaaca atttaacgaa
     3841 ttacaaagta gctatgcaaa gactatgttt cgattactaa aacaatatcg aactaaaggt
     3901 tttgcttatt tttctaaaga ggattttcat gaactattag atattccaaa aagttactgg
     3961 aacaaccaag caaacgttga taatcgtgtt ataaagccta tacgacagga attaaccgca
     4021 atcttcaaag gattatcaat caaaaagaaa tacggaaaag ggcgaggaaa acctgtcgtt
     4081 ggctatcaat ttacgtttaa accagaaatc aaaaaagcag atgattttaa taaacatgca
     4141 ccaaccaaag aagaacaaaa taaagcattg gctttagctg ataaacaaaa agaatacgaa
     4201 gaacctgcta caacaattaa cgaaaaaatc aaacagaaga aacgagaact tgaactcctc
     4261 caacaaatca aacaactaga aaaagagaaa gaagctcttg aaaaacaaac agagtccgca
     4321 ctttcgaaaa aagagaaaaa aagtttatta tctgatttag ataatttatt ccagtagcaa
     4381 cctaggaggc aaccagtatg caaccacaag aaacctttac aataaaggaa ttgtcagatc
     4441 tttttaagat gagtaggcaa gcaatatcga aacatatcca aaaattagat tcttccatga
     4501 tagctaaaaa tgagcgtggt tataaggttg tattgcgttc tggagtattg caacttgctc
     4561 gcaacttagg caaccaaatg ttgctagata ggttgcacga gtacgaacaa gacaacgata
     4621 aaaatgttaa tataccaagc aaaaacgtgg ttacaaacaa gttgctagat cagttgcgtg
     4681 aagaaaatga atttttacga gaacaacttg ccctcagaga gcaaaataat tctcatatac
     4741 aaaaactact cgaccagcaa caacaactca ccctacaagc caacaaacaa atcgaaaaac
     4801 tgcaagaaaa actacagttg acttatacag aagaagctcc agagaatgac tcaacgactc
     4861 tttctgaagc aacagaaaca atagacaaac aaattgatac tgaaaaaaaa tggtggcaac
     4921 tttggaaata actggaggga acattcatga caagaaaaaa tcttctattc ggttctattt
     4981 tactaggaat tctaggattc agtccgatgt tattgtcagt gatcaataaa gggaataaac
     5041 atggtgaatt gaaattgaat ccaaattatt acaacagtcc taacgaagaa ttgtttgtaa
     5101 aagaaacaga taaataataa aaaagactgc tctttcgagc agccagttaa aaaataatga
     5161 gtgtttattc ttgaaaaagc aacgcgctta tgaagcgcta ccaaaaattt atcatatttt
     5221 attaaaaata acaacccagt aaaaaatgac tggtaaaaat tcacaaagaa acggaaagaa
     5281 gcaaccgcag aaaatcaagc ttaaaaagag cttttgacct agaccaatcg aaaatcacaa
     5341 agtgagcctc tgtatcggtt gatacaaggg ctacttggca tagtcgacat tatgttaaca
     5401 agaatttaat ataacatatt ttcctaatgc attctaacta taccacatta tcaaaaatat
     5461 aaattatctt tactaaataa aatattgaca agcctatacc ttttgtttta tgattttcac
     5521 aaagaaaacg ttttctatat atttttctgt atcaggagga aattctatga aaaaatttac
     5581 tttaggggta gctttgttaa ctattggggc agttgtttta ccgtcaacta ctatagttta
     5641 tgcttcagaa aattcccaag tggtatcatt aaacgaaaac aatgctactg aaaatgtagt
     5701 tattgagtac actgaagatg gtatggtcgt aactaaatat gaggatggtt atagtgcgga
     5761 agattttgca aattcttcag atatcatgac aagagctgca gttgcaagat ggggcaaaaa
     5821 ggaatataca aatattgctg taaccactgg agtagctgct aatgctatta atgctgcctt
     5881 ttgggcagga gggaaagcta gcacttctat gtttggtatc ccaggttggg caattggtgc
     5941 tttgttaaca ggtgcaagtt ggactaaaaa aggtgcttct ccagggaatg cagttgcaaa
     6001 agaatgggac aaaaatggta atggctggat tggattttat taccaaaggg gatatgatgc
     6061 agctggaaga gttgtagcta ctagatattc aacgttataa gttaaggagt gatgtttttg
     6121 atatctaaat cattaaaatc attaaaaaca tttactaaaa agaatccgca aatagctact
     6181 attcgtattt ttatatttgc tatagctgtt gctataattg ttaatatgct aagcggaggg
     6241 caattataca aatctggttt atttttagtg ttaatcttag ttgtcttaga cataataaag
     6301 tct
//
LOCUS       DAKBVG010000146         6250 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000146 DAKBVG010000000
VERSION     DAKBVG010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6250
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.146"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            <1..2175
                     /locus_tag="OUD09_002051"
     CDS             <1..2175
                     /locus_tag="OUD09_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCT9693293.1"
                     /translation="FDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSGGYR
                     YPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDFRYD
                     SYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVIIRST
                     QLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLDSKR
                     GSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGINSFN
                     FGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLINNWE
                     ATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLACGLE
                     NLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDFSRQ
                     DVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLYEFL
                     EKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVYPIS
                     SMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAFYKK
                     HRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLAGLA
                     EEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            2236..3456
                     /locus_tag="OUD09_002052"
     CDS             2236..3456
                     /locus_tag="OUD09_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HCT9693294.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            3453..4913
                     /gene="gtfA"
                     /locus_tag="OUD09_002053"
     CDS             3453..4913
                     /gene="gtfA"
                     /locus_tag="OUD09_002053"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HCT9693295.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            4990..6096
                     /locus_tag="OUD09_002054"
                     /pseudo
     CDS             4990..6096
                     /locus_tag="OUD09_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT         2156 a         1005 c         1230 g         1859 t
ORIGIN      
        1 tttgacaatc acacacaaac atttcattta caaaataaaa aaattagcta tctattagga
       61 atcgaagaaa ataattattt aacgcatctt tattttggaa aaaagattac gcactattct
      121 ggtgggtatc gctacccccg aacagatcgt tccttctcac ccaacaatgc aaatgcgcaa
      181 gatcgtttat tatctttaga cacattacca ttagaatttc caggatatgg acatggtgat
      241 tttagagaac cggcgcataa tattaaatta ggaaacggta gtcgtgtaaa tgattttcgt
      301 tatgatagct acgaaattca aaaaggaaag cccagcttag cgggattgcc agccttatat
      361 acggaagatg atgatgaagc tgaaacgctg attattcaat tgaaagacgc gattagtgga
      421 ttgactttac agttgttcta cacaatttat caaaatcaca gtgtaattat tcgttctacg
      481 caattagtaa ataatagtga tgaaacagtg gagattaacc gcttagctag tcaatcaatt
      541 gattttccaa atcgtccact cgagttaatt catttaaatg gtacctgggc aagagaacgt
      601 caaatgacta gagaaaaaat tcatacaggg ataaaagtat tagatagtaa acgcggatca
      661 agcagccatc aacaaaatcc gtttgttgct ttagtagatg ccaaaacaga tgaatttcaa
      721 ggagaggcat atggatttaa tttagtttat agcggtaatc acgaaacggt gattcaaaaa
      781 gatccatttg accaaacacg ggtcattatg ggaattaatt catttaattt tggttggcaa
      841 ctaaaaactg gcgactcatt tcaaacgcca gaagtcgtga tggtttatag taatgaaggt
      901 ctgaatacgt tatcacagac gtatcatgaa ttgtataatc accgtttaat tcgtggaaac
      961 tatcgtttaa aagaacgtcc cacgttaatc aacaactggg aagcaacgta ttttgatttt
     1021 aacgaagaaa aaatcatgaa tattgtcgat gatgcgaaga acttgggcat tgaaatgttt
     1081 gttttagatg atggttggtt tggcggtcgt gaaaatgact tcacttcatt aggtgattgg
     1141 gttgaaacag aaggcaaact tgcttgtggt ttggaaaatt tagccaagca agtgcatgat
     1201 aacgggatga aatttgggat ttggtttgaa ccggaaatga tttctgaaga tagtgaacta
     1261 tttcgagcac atccagattg ggcattgggt attccaggtc gtgaccgctc aattagtcga
     1321 aatcaatatg ttcttgattt ttcaagacaa gatgttcgtg ataatatcta tcaacaaatg
     1381 acggcgattt ttgatcgtgt accgattgat tatgtgaagt gggatatgaa tcgaaatatg
     1441 acagaagtct attctgtctt gttggactca acgatgcaag gtgaagtggc tcatcgttat
     1501 atgttagggt tatatgaatt tttagaaaag ttaacaacga attatccaac tattttattt
     1561 gaaagttgtt cgggtggagg cggtcgtttt gatgcaggtt tcttgtatta catgccacaa
     1621 acttggacga gcgacaatac cgatgccgtg gctcgtctga aaattcaata tggaaccagc
     1681 ttagtgtatc caatttcaag tatgggtgcg catgtttcag cagtcccaaa tcatcaaaca
     1741 ggaagagaaa cgagtttaac tattcgtggc aatgctgcga tggctggtgt ttttggttat
     1801 gagctagatc taacgcaatt aacttctgaa gaaaaagcag aaattaaaga acaagttgcc
     1861 ttttataaaa agcatcgcca acttttgcaa tatggaactt tttatcgctt ggaaagtccg
     1921 tttgaaggca atgatactgc atggatgttt gtctcaaaag accaaacaga agccatggtc
     1981 ttttatttcc gagtattagc agaagctgcg catccattag tcacattgaa attagcagga
     2041 ttagcagaag aagcaactta tcaaatggaa gatgtaacat ttgctggcga tgaattgatg
     2101 aatctcggtt tttatattga ctcagaacta catggtgatt atgcgacaca acgcttttat
     2161 ttgaaacaga tatagtagct tgaatttaca atataagtgc aacaccaaaa gagactcatt
     2221 ccaaacgaag aaaatatgtt ttatttacac gcatttacta aaaagcagcc tgatttgaat
     2281 tgggaaaatg aagaagtacg tgaagaaatc tatgcaatga ttaactattg gttagataaa
     2341 ggactaggcg gatttagaat cgatgcgata ttaaacatca aaaaaaaact agagtatggc
     2401 atctttgaat ctgatggaga agatgggtta gccttcattg ggccctggat tcttaatcag
     2461 tcgggcattg aagtgtggtt aaaagaaatg agagagcgga catttaaacc acataatagt
     2521 atgacggtag cagaagccga tgttcctaat gaacgtttag atgaatatat tggtgaagat
     2581 ggctattatt cgatggtttt tgattttagt tatacggata ttgatgtacc tgaaactggt
     2641 gaatggttta aagatagcca atggacatgg acggatatgc gtaccaatat ttttacgaat
     2701 caattagtta cgcaagataa aggttggggc gccttatatt tagaaaacca cgatcaacca
     2761 cgttcaatta ataaatatat tccagaagaa tggatcaatg attatagtaa aaaaatgttg
     2821 gcgacgcttt ttatgttact aagaggtacc ccttttattt atcaaggaca agaactagga
     2881 atgaccaaca tagaaatggc atcattagaa gattttgatg atgtagctac gcattctcag
     2941 tataaacggg cattagaata tggattggca ccagagcaag cattgaaagc tgtcaataaa
     3001 cgaagtcggg ataattctcg cacacctatg caatggaata cgcaaaaaaa tgccagattc
     3061 tcaacttctg ataatgtatg gttaaaagta aatcctaatt atccagaact caatgcagaa
     3121 gctcagttaa ccaatcaaca gtcggtattc aattattatc atcaattaat taaattaaga
     3181 aaaaatagtg tatacaaaga cgtgctaatc tacggacaat ttctaccagt gaaagataaa
     3241 aatgaacata ttttgcttta tgaacgccaa ttagaagata aaatcgtatt agttatctta
     3301 aacgttacag caacagaaca agaatattat gtagatgaac agtaccaaca agtattaatc
     3361 aataattatg aagatgtttt attagatcaa agtcgaaaat tgcgtttacg accgtttgaa
     3421 agtattgtat tagctaatta tggaggacaa ttatgaaaat taaaaacgaa gcaatgctca
     3481 tcacctatgc agatagttta ggaaaaaaca tgggcgaatt aaaacaagtc ttagacaaac
     3541 atttacaagg tgttattggt ggtgtacatc tgttgccttt ttttccatcg acaggtgatc
     3601 gaggctttgc gccgagtgat tatacgacag ttgatccagc tcttggaaca tgggaagatg
     3661 tggaagcatt aggtgaagat tattatttga tgtttgattt tatgattaat cacatttctc
     3721 gtgaatcaaa atttttccaa gatttcaaaa aaaaccatga gcagtcacca tacaaagaaa
     3781 tgtttatccg tattcatgaa tttttcccag aaaatcgacc aacacaagaa gatattgatt
     3841 taatctacaa gcgtaaagac aaagcgccgt ttcaaacggt agaatttgcg gatggtacga
     3901 cagaagaggt ttggaatacc tttggggaag aacaaattga tttagatgtg accaaagaag
     3961 ttgtgaaaga atttattcga gaaaccatca aagatatggc gaaccatggc tgttctttaa
     4021 ttcgattaga tgcgtttgct tatgcgatta aaaaattaga taccaatgat ttcttcgtgg
     4081 aaccagatat ttgggaactt ttagacgaag tgcgtcaaga agcagcgaaa tatgacgtcg
     4141 aattattacc agaaatccat gagcattatt ccattcaaat gaaaattgcc aatcatgatt
     4201 attatatcta tgacttcgcc ttgccaatgg tcactctgta ttcactttat agtggaaaat
     4261 cagaacgttt agccaattgg ttgaaaatga gtccgatgaa gcagtttaca acattggata
     4321 cgcatgatgg aattggtgtt gtggacgctc gtgatttatt aacagatgaa gaattggatt
     4381 acacctctga ggaattgtat aaagtcgggg cgaatgtgaa gaaggtctat tcaagtgcca
     4441 attacaataa tttagatatt tatcaaatta acagcaccta ttatagtgca ttaggcgata
     4501 atgaccgtag ctatctgtta gcacgtgcga ttcaagtatt tgcgccaggg attccacaaa
     4561 tttattacgt tgggttatta gcaggaaaaa atgatattga actgttggaa gcaacgaaag
     4621 aagggcgtaa tattaatcgt cattactatg atttagccga aattgaagaa gaagtgcaac
     4681 ggccagtgat tcaacaatta tttgcacttt taaaatttag aaatgaatct gcggcgtttg
     4741 atttggatgg aacgattgaa gtagcaacgc catcagaatc agaaatagtg attacacgta
     4801 aaaataaaaa cggtagtgat agtgcaacat taacggctaa cttggatacg aaagaattta
     4861 tcattgagtc taaagatata aaaattttca ctagtgttgc attaaaattt taacctattc
     4921 catagaacaa aaaatccttt atactagcac ttggatgact tatccaagac caataaaaag
     4981 gactcataca tggataagta taaaacaaat acagcattta ataaatggtt ttctgccata
     5041 aaattaggaa aactttctgt caactcccaa aaatgtattt ttgattacaa ttcgtatcgt
     5101 aaaaaactgt cttttgaagc tgtgctcaag ctctttctct acgcagttaa tgaagaaaaa
     5161 gaaagtttgc gtgatttgag tacagctctt atcgaccgat cgctccaact agaaaccgat
     5221 gtgtcatcga ttagtcatac ccaattgtct cgagcattta atgcacttga acctaaagtg
     5281 ttagaagaaa tttttcaaca actgttagaa aaagttcaac atcaaatcaa accaaccaag
     5341 cgaaatcatc tgtatctgat tgattccagt acgttttcgc ttagtttaaa acgtcataag
     5401 tgggcgactt tccgtcaaac aaaatcaggt gtcaagttac acttaaacta ttgttatatg
     5461 gacgatacta cgatgtatcc aactgatttt accttaacaa atgcccatga acatgatctc
     5521 aatcaactac cagtattggt gaatcaacca gaggcaactt acgtgtttga tcgtgggtat
     5581 taggactttg aaaagatgga tcagatgcat tgggatggct atttcttcgt cactcggatt
     5641 aaaaaaaaca ccaaagttca cgtgatagat actctggaaa catcgccaga aacagaaatt
     5701 ctacgagacg agctggttcg tttaggatct aaaacctatc taaccgccaa tttccgacta
     5761 gtcactgttc aagataagaa cggacgtgtg tttcaattta tcaccaatcg aatggacgtc
     5821 tcttcaaaag aaatctctga tatgtatcac gctcgttggc aaatcgaatt gtttttcaag
     5881 cacatcaaac aacacatgac cattaaaacc ttcttttctc aaagtgaaaa aggcgtgcag
     5941 aatcaattga ttttaacgat gatttccgct ctcctcacgt ttctcatcaa attggagacg
     6001 aaaacggaaa aatcagtgtt ccaaatcaaa cgattctttc gttatttact gtttcagccc
     6061 tttgaatgtt gtctagaaaa attaattccc acatgagaaa aaagaaattt ggctcgttac
     6121 ttttggtttt cagttgaata acagataaaa aattttctgg aaaaagtcat cctctgcgtc
     6181 agcacagttt tttttgactt attttcagag gaaatagttt tcagaatatt ctaaaatgtt
     6241 ttatgcaaca
//
LOCUS       DAKBVG010000147         6209 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000147 DAKBVG010000000
VERSION     DAKBVG010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.147"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            <1..651
                     /locus_tag="OUD09_002055"
     CDS             <1..651
                     /locus_tag="OUD09_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCT9693296.1"
                     /translation="LFFKLAILYFTESGEKVRRRKLIANDILIILKNEFGIEVKDYFI
                     DLDLLQLINSVKRMYSGDDNGVDLYKFVTKHIFVNLNIDEKNYPSIFKHYLFFINEVE
                     DRIKNNSLSSSVSSFKKMFKSRDGITLTTAHKVKGNEYDTVIAINLLNGKIPHWNEIF
                     NVSDKGISSARKLLYVICSRAKNNLFLFSEVGYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            851..1165
                     /locus_tag="OUD09_002056"
     CDS             851..1165
                     /locus_tag="OUD09_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCT9693297.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            1186..1563
                     /locus_tag="OUD09_002057"
     CDS             1186..1563
                     /locus_tag="OUD09_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCT9693298.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            1573..2346
                     /locus_tag="OUD09_002058"
     CDS             1573..2346
                     /locus_tag="OUD09_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693299.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            2368..3771
                     /locus_tag="OUD09_002059"
     CDS             2368..3771
                     /locus_tag="OUD09_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HCT9693300.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            3953..5137
                     /locus_tag="OUD09_002060"
     CDS             3953..5137
                     /locus_tag="OUD09_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HCT9693301.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            5134..5424
                     /locus_tag="OUD09_002061"
     CDS             5134..5424
                     /locus_tag="OUD09_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693302.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            5421..5642
                     /locus_tag="OUD09_002062"
     CDS             5421..5642
                     /locus_tag="OUD09_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693303.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            5684..>6209
                     /locus_tag="OUD09_002063"
     CDS             5684..>6209
                     /locus_tag="OUD09_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693304.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPR"
BASE COUNT         2119 a         1012 c         1209 g         1869 t
ORIGIN      
        1 ttatttttca agttagcaat tttgtacttt actgaatcag gtgaaaaggt tcgaagaaga
       61 aagctaattg caaacgatat tttaattata ttaaaaaatg aatttggaat agaagttaaa
      121 gattatttta ttgacttaga tttattacag ctaataaaca gtgtgaaaag aatgtatagt
      181 ggagacgaca acggtgttga tttatacaaa tttgttacca aacatatatt tgttaatctt
      241 aatattgacg agaaaaatta cccgtctatt tttaagcact atctattttt tattaacgaa
      301 gtagaagatc ggataaaaaa taattcatta tctagtagtg tatctagttt taagaagatg
      361 tttaaatctc gagatggtat cacacttact acagcacata aagttaaagg aaatgagtat
      421 gatactgtta tcgctattaa tcttctaaac gggaaaattc ctcattggaa tgaaattttt
      481 aatgtttctg ataaaggaat tagttcagcg agaaaactac tatacgtaat ttgttcaaga
      541 gcaaaaaata atttatttct tttctcagag gtgggttatt ttacgagtaa aggagacttg
      601 ctttatccca ctcaattatt agataaaata gaatttgatt ataatattta aaatacctag
      661 gtagtcgttg ttttaaatat agtaggaatc ggacgctcag tacggagcgt ctattttttt
      721 acccaatttt gaaaggaagt gaacttatga aaataaaaaa tcaagaatca aaaggtcgct
      781 ctcccccctt taaaaccatc acacaaataa gattcattca aacataaaaa cagaaaggat
      841 aggtaaaaat atggaactta aatatgtcat tcccaatatg gaaaaaacat ttggacaatt
      901 agaatatgct ggtgaaggga acattgaaca acgtcgaata aatggcagac caaccattct
      961 ctctcgtagt tacaatttat actcaacgat tcaacgagca gatgatattg ttgtcattct
     1021 tccctccgaa gctggcgaga aacattttga atccgacgaa aaagtcaaac tcatcaaccc
     1081 acatattacc gcagaaggtt ataaaattgg caatcgagga tttaccaatt acattatgca
     1141 cgcagacgat atggtgaaag cataagaaag gaaaggtgaa aaaatatgag attagcagaa
     1201 ggtattgtga ttgaaaaaga ccagacattt ggcacattga aattttccgc cttgcgtcgt
     1261 gaggttcgta tccagaatga agatggtacg gtttccgaag aaattaaaga acgtacctat
     1321 gacttaaaat caaaaggaca aggacgcatg atacaagtca gtatccccgc ttctgttcct
     1381 ttgaaagaat ttgactacaa cgcagaagtg gaattaatca atcctgtggc agataccgta
     1441 gcgacggcta cttttcgagg ggcagacgtt gattggtacg tgaaagcaga cgatcttgta
     1501 ttaaaaggac agacaaaacc tcatcagaaa tataaaaaag aagcgtccga aagtgaaaaa
     1561 taggtgttgc ttatgacaaa cacaccattg attttgaaag aaattccgaa ggatgaagcc
     1621 atttctttta ttcggcaata ccattattca aagatactcc ctagattgtg taaatacttt
     1681 ctagggattt tttcagaaga aaaattatta ggcgttgtgg aattaggctg gggtacgcaa
     1741 cccctacaaa ccatacgcaa actctttccc gacagttccc ttcaaaccac agattattta
     1801 gaaattggaa agatgtgttt tcttccagaa atgaatcaaa ccaattattt tggcagtcaa
     1861 gctctttcag ctctaattaa gtggttgaaa gaacatacgg actgtcattt tttatataca
     1921 ctggcagatg gaattgaggg aaaatgtggc tatgtctatc aagcgtccaa cttcttttac
     1981 tgcgggtatt tcaagacaag tgtctatcgt gataagcagt catgggaaaa gatacaccct
     2041 cgaagtgctc gccttttatt agaggaaaat gctcgctttg aacaggtaga gaaaaagcat
     2101 tggctttccc aggcattttg cgaatacaaa gggattgaga aaatcaatgg acgcatgttt
     2161 cgctatctct atccgttgac aaaagaagcg aaaaaactgt taggacacac tctctatcga
     2221 cgtcattatt atccgaaaga aaaaaattta cgctttgaaa agcgaatcgc ttatcagaaa
     2281 tatgaagcca tttctcaacc aacttttgat aaacaggctc gtatctacaa tacacaatta
     2341 ttttgatagg tggaaaggag gatttttatg ccattgcttc atgacagagg agagcgaatc
     2401 aaagccaacg ataagcactt ggtctatcat tttgtactaa tttcattgct ggtagttttt
     2461 ctagtcgtcc tactcccttt tcattggaga gcgttacaat cggtgaactg gcaagcagtt
     2521 cagttagata catttttcta tcaacttcat acgccatact tttggagcag tattctcact
     2581 gctctttttg tatgcggact tatcagctgg ctttattatc actatcgtat agattctata
     2641 aaacagttag agcaccgtca aaagttagct cgtatgattt tagaaaatca ttggtatgaa
     2701 gccgaagaaa ctcaatcaga gggattcttt aaagatttac ccagcaagaa aaccaaacaa
     2761 cgaatccgcc attttcctaa aatatattat cgaatgaaag atggactaat ccatattcat
     2821 gtagaaatca ccttagggaa atatcaagat cagcttttgc acctagaaaa caaactagaa
     2881 acaggattgt attgtgaatt agtggaaaaa acgttgaaag attcttatgt agagtatgtc
     2941 ctactttatg atactattgg caatcgaatt ggcattgaag acgtggtagc tcaagatgga
     3001 cgtgtcaaat tgatggaatc ggtctattgg gagttcgatt ctctccctca catgttaatt
     3061 gctgggggca ctggtagtgg aaaatcctat tttattctca cattgattga atctctcctg
     3121 catacaaatg caaagcttta tattcttgac cctaaaaatg ctgatttggc agacttaggg
     3181 gcggtcatga ataatgtcta ttaccgtaaa gaagatatgt tggcatgtat caatcaattt
     3241 tacgaagata tgattgatcg cagtgaaacc atgaaacaac accctaacta taaaacagga
     3301 gaaaattatg cttatcttgg tttgtctgcc aattttctta tttttgatga atatgtggca
     3361 atgatggata tgctaggtcg tgaaagtacc accgttattc ataaactcaa acaaattgta
     3421 atgttaggac gacaagcagg cttcttttta attcttgcct gtcaacgtcc agacgcaaaa
     3481 tattttagtg atgggattcg ggacaacttt aacttccgtg tggctttggg tcgtatgtct
     3541 gaaatgggct ttggtatgat gttcggaagc gatacacaaa aacaattttt cttaaaaccc
     3601 atcaagggaa gaggttacgt ggatacagga aaaagtgtga ttagtgagtt ttatacaccc
     3661 cttgtaccaa aaaggtacga tttcttagga gaaataggaa aagtcattca aaaaaagcaa
     3721 tctgagcctg taccgcacga agtgaaaggt acaggttcag acttgctttg attggtgtgg
     3781 cgttagccac gccaatcctt tagccccctc tatctaacag gggggcacaa atacaactga
     3841 aaacaggcaa ttctcatcaa ccaatgttga tggtacaaag gattgcggaa aatatgaaag
     3901 gtgtaaactc aagggtcata gtttacatct tttttagatt ggaggtttgc gaatggaaaa
     3961 cgaaaaaatt tggattaagg aattgaaaga aaagcgactg gcttatggcg tttctcaaaa
     4021 taaactggcg gttgcttctc atattaccag accttaccta tcggatattg aaacaggcaa
     4081 ggcagtccct actcaacagg tgaaagaaga tttactgaac gctttggaac gcttcaatcc
     4141 cgataaccca ttggagatgt tattcgacta tgtacggatt cgattcccta ccaatgatgt
     4201 ggctcaaatt atcggtgagg ttttacgttt gaatatggat tatatgctac acgaagactt
     4261 tggctattat tcttatcccg aacactatcg tttcggtgat attgttgtcc tagtctctca
     4321 tgatgtaagt aaaggtgttt tattagaact gaaaggaaaa ggctgtcggc aatttgaaaa
     4381 ctttctattg gctcaacatc ggagctggta tgatttcttt caagattgca tggaacataa
     4441 aggtattttc aaacgcttgg atttagctat caacgataaa acagggattt tgaatattcc
     4501 agatttggca aagaaatgta aacaggaaga atgcatttcc gtctttcgaa gctttaaaaa
     4561 ttatcgcagt ggtgaattgg ttcaccgtga tgaaaaacca tacatgggaa acacacttta
     4621 tattggctca ttaaaaagtg aagtctattt ctgtatatat gagaaagatt atgagcaata
     4681 cgtgaaaaat gatgttccct tagaagacgc agaagtaaag aatcggtttg aaattcggtt
     4741 aaaaaatgac cgagccaccc aagccatgaa agatttattg gcacatcaac aggcggaaaa
     4801 aacagccttt gaaattatca atcgctatat ccgctttgca gataaagacg acactaaacg
     4861 tcggagtgat tggaaaacta atgaacgttg ggaatggttt atcggtaaaa atcgtggagc
     4921 attacgccta accacacagc cagagcctta ttcttttgaa cgaacattga actggttaca
     4981 tcatcaagtc gcccctacat taaaaatcgc aagcatttta gatgttctca acggtacaac
     5041 cattatctcc accatgattc aggaagccaa actgacagaa aaacatgaga aattaattga
     5101 acaacaacat ctagccatgg aggatttgat tacatgagcc ttttaacaaa agaattaaag
     5161 aagttagggt ttcagtgtgg cattgagttt caagcgtata tacaaaatac agggaaatat
     5221 acgtctctca ttattgaggg aaaacggcaa gctggtgata ccatctatac ctatgatttt
     5281 tacaaggtga gattttataa taattatacc aatcgagtaa ccgtctatgg agagcattta
     5341 accccttttc aattattaag gcgtgtaaaa agctatatct attatcgaga aaaatatctt
     5401 aaggaaagga ggacaatcac atgaattttg ggcagaattt gtacaactgg ttcttggata
     5461 atgcacagtc attggtattg ttagcgattg ttgtcattgg actgttttta ggttttaaac
     5521 gagaattttc aaaactcatt ggcttcttga ttattgctct aatcgccgtc ggacttgtgt
     5581 tcaatgcagc tggcgtgaaa gatgtgttat taaacttatt taaccgaatc attggtgctt
     5641 aacagatatt tttcatgata aaataggaag tatatatttt gttatgaaag gatttatgaa
     5701 tatgtttaca aatgatcaat ttaaatctct tttaattaaa acattagaaa gaaagagctc
     5761 ttcaaattat tatgaaggag gtaacgagat tgtttctaaa atgaaaatat cagaggtaac
     5821 tttagatgag actgacgatt ttacttatta taaaggatat aaaaagggct ggaatacgct
     5881 ttgcacatat cttaaaataa gagttccttt tgatgattta gacttttttg aaagtcataa
     5941 agatttgatt acccagacag caagctccat atatgataaa caaggtgata atgtattagt
     6001 ggatactatt ttagtaccac ttccagagaa ttacgaagta attaacttta gtcagttgaa
     6061 aattagtgat gtggtttcac aagctattga agacgctgag tcttttatga gtaatggtga
     6121 atatcaacgt gcctttgaca gagttcacac tgcttttcat ggatatttaa ttgaaatact
     6181 aaaaaaatat gaaattacag ttcctagag
//
LOCUS       DAKBVG010000148         6114 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000148 DAKBVG010000000
VERSION     DAKBVG010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6114
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.148"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            15..176
                     /locus_tag="OUD09_002064"
     CDS             15..176
                     /locus_tag="OUD09_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693305.1"
                     /translation="MKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            173..1477
                     /locus_tag="OUD09_002065"
     CDS             173..1477
                     /locus_tag="OUD09_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCT9693306.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEDTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            1573..2442
                     /locus_tag="OUD09_002066"
     CDS             1573..2442
                     /locus_tag="OUD09_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCT9693307.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(2554..2874)
                     /locus_tag="OUD09_002067"
     CDS             complement(2554..2874)
                     /locus_tag="OUD09_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HCT9693308.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            3044..4003
                     /locus_tag="OUD09_002068"
     CDS             3044..4003
                     /locus_tag="OUD09_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCT9693309.1"
                     /translation="MTYTHLTPTELVMIEAYFNQSHSVSKVAHLVQRSRQTIYNVYRF
                     LKSGGSAISYYEQYKKNKRNCGRRPIVLPDGQQEYIQKKVAQGWTPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDPHDLPMKGKRKPNGHKEKRGKQAFRRSIQDRTTDYIQFNHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPAGRQAIDIENRLNQWFQSVPKHLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNK
                     VSETFIQSVASKRNHIPRKSLHYRTPLEVFLSYVGKDDLSSLI"
     gene            4042..4488
                     /gene="dhaM"
                     /locus_tag="OUD09_002069"
     CDS             4042..4488
                     /gene="dhaM"
                     /locus_tag="OUD09_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HCT9693310.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            4463..5473
                     /gene="dhaK"
                     /locus_tag="OUD09_002070"
     CDS             4463..5473
                     /gene="dhaK"
                     /locus_tag="OUD09_002070"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCT9693311.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            5501..6106
                     /gene="dhaL"
                     /locus_tag="OUD09_002071"
     CDS             5501..6106
                     /gene="dhaL"
                     /locus_tag="OUD09_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HCT9693312.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
BASE COUNT         2053 a         1103 c         1325 g         1633 t
ORIGIN      
        1 atgaagatga aaaattgaaa aaaagacaag aattacttga agaagtgtat acggaacgtt
       61 ttggaacaaa agaagagaga gaaacggtcc gtttttattc tgtctccgaa gagaaaaatc
      121 tagatacgac attcattgca gatttgtata aagaaaatgg ggtggaactg aaatgatctc
      181 aatcacccat acaggtaatt tattcttaga tacttgtttg tctattatgt atttttttct
      241 cgtttcttat ccgattttag gcggatttgt atggtttatt ggtgtctggt gttatgtgtt
      301 tttatacaaa cataagcaaa aagaatgggt agatgttccc ttgagtgtcg aaccgttcat
      361 cacgatcatg gtgccagccc ataatgaaga aattgtgatc gaagatacaa tcgagtattt
      421 gatgacgaaa ttgaattatc acaactatga ggtattagtg acagacgatg gttcaacgga
      481 ccaaacacca gaaatattag ctagactgat gaaaaaatac gctaatcttc gtgtggttcg
      541 gatcgaaaaa aataaaggaa aagcccacgc gtttaatatc ggacttgcct ttgcaaaagg
      601 gaaattgatt ttaagcaatg atgcagacac ggtaccggag ccagatgcac tgattaggta
      661 tgtgaattat ttcattcgcc ctggtgcaag acatattgct gcggtcactg caaatatgga
      721 tgtccaaaat cgtaccaaat tgattgcaaa atctcaaacg gttgaattct caagtatcgt
      781 gggcatcatt aaacgaacac agtcagctgt tttcggtggt ctttatgcgt acagtggagc
      841 aaatacgatg tatcgaaaag aagccttgat cgatgtgggc ggattcagac aagatcgagc
      901 gacagaagac atcagtattg cttgggatca tcagttaaat gactggttat ctgtttttgc
      961 tcctgagatt atctttttca tggaagtacc tgtgacgttg aagatgctat atcgacacag
     1021 aaaacgttgg gcaaaaggtg gaacagaagt ctggctgacg aatttcaaaa aagtgatgct
     1081 ccatcctttt aaacatattg gaagaacgat catcttcatt gatcaaacat tgagtattgt
     1141 ctggtcaata ttcttttgta tttccgtagt tctttttgct ggattaatcg ggcattatgt
     1201 atatcaagga aactacgaac agatttatat tacttttaca ttcagttttg tgttcatctg
     1261 ctttgaaatg gtcgcaggat ttttccagtt gctagcttcg ctgattgtag atgatcgacg
     1321 aagaaaattg aaataccttc ttttcgcacc actatatatg cttttgttct ggatagtgaa
     1381 cgcgataacg atcgtgacga ctttcattcc agcagtcaaa acgattttag gttatggaag
     1441 cgggacatgg aaaagcccgg aacgtacaaa aaaataaaag atgcaccgtt ttttagacaa
     1501 acagcctgtt gtgtttatgg ttcttccaaa catttttgct tataataaga aattagttaa
     1561 aaagtagtaa ggatgggaaa agtgatgaaa aaaagaatga agccgttaat ctggccaatt
     1621 gctgtttcca gtattttctt attgggacag acagcttttg cggaaacttc tgaacctgct
     1681 caaacagaac cttcaacaac cataagctct tctggtgctg aaaacggtgc ctcggaaaca
     1741 gaaatgactt ccaatgtacc gacagataat tcaggagaaa cgatttcatc ttcagaagat
     1801 acatcaataa atagttctac ctcttctgac caaatcgatc atttgcaaga cttggaaaat
     1861 aaaagaaaag aactcgaaga agcattagaa gctgcaaaaa acaaattgaa agaagaccaa
     1921 gcagcaatcg atcaagattt agctgatctt aaaaataaag tgggaaatga ccgtgcacaa
     1981 cttgagaaaa agcttgccga agcgaaagca aaagcggaag ctgtccaaac agatcttgac
     2041 gggaagatcc aacaagcaaa agaattcaat cagcaattaa atcaggttgc tcaaacagct
     2101 tctggaaagc taagtcaaac tcaagcggat atcacagcgg ccagtcaagc aatccacgga
     2161 aagatcagcg aagcacaaac agttgctaat gattttgaag caaaaaaagc tgacttagca
     2221 gctagtttcc aacgaataaa agatattttg cattcggcag gagaaaaatc agtgacctct
     2281 ggaagcacga cagaaacagt tcagcaaacg acagagccat ccaaatcaac aagtcagact
     2341 ttgccaaaaa caaatgaaaa aacagcttct gtcagttatt ccttagcagg ggcgggtgtg
     2401 ttagtcgcag cagcttacgg aatcatccgc aaaaagaaat aaaaaagaga ggatgggaag
     2461 gtagagtacc tcgaagttag actgaaaaat ctaattcttg agtgccacta catgtcccat
     2521 ctctctttag ttattaaaac agatgtcccc tacttatctc gtggagacag ctgttccact
     2581 acatgtgacc atcagcatgc cattgtcgct gcctaatact tcatagtcca tttttacgcc
     2641 aatgatcgca tcagctccta atgcttttgc acgttcggcc atttcggcaa gtgcttcttc
     2701 tcttgcaccc attagttcgt tttcatagct ttttgagcgg ccgccaaaga tatttcgcaa
     2761 ccccgcctcc aagtctttaa atgcatggat cccagtaatg acctcaccaa aaacgatccc
     2821 tttataagct actacggctt ttccctctac actgttcgtt gttgttgtaa tcattcgatc
     2881 actcctctct tttaattaaa gtataacaaa cttcaagaag cactacacat attttcccaa
     2941 aattagaaat cgccgattgt aaaattaagc tagacaacta aaaaagtcat ttgtgctaca
     3001 ctcaaaatag ttccgtcgaa agaattataa ggagtgagta caaatgacct atacccatct
     3061 tacaccaact gaactcgtaa tgatagaagc ttatttcaat caatcacact ctgtttcaaa
     3121 agtagcccat ctggtacaac gttctcgaca aaccatttat aatgtctatc gttttcttaa
     3181 atctggtgga agcgcaataa gctactatga acaatataag aaaaacaaac gtaactgcgg
     3241 aagacgtccg attgtgttac cagatgggca acaagaatac attcaaaaaa aggtggctca
     3301 agggtggact ccagatgtcc ttattggtcg agcagagttt cctattgctt gttcggtacg
     3361 tacactatat cggatgttta aagataaaac ctttgaccct cacgatttac caatgaaagg
     3421 gaaaagaaaa ccaaacggac ataaagaaaa aagaggaaaa caagcttttc gccgttcgat
     3481 tcaggataga acaactgatt atatacagtt caatcatgag tttggtcacc tagaaggtga
     3541 caccattgtc ggtggaaaac ataaaagtgc ggtgatcacg ttagttgagc gattatctaa
     3601 agtgatcatc accttgaaac ccgctggtag gcaagctata gatattgaga atagactgaa
     3661 tcaatggttt caatctgtac cgaaacactt gtttaaatcc atcacatttg attgtggaaa
     3721 agagttctcg aattggaaaa agattagtaa taccaatgat atttcgattt atttcgctga
     3781 tccaggaaca ccttctcaac gaggactaaa tgaacattcg aatggcttat tacgtaaaga
     3841 tggcttatat aaagctatgg attttaataa ggtatccgaa acctttatcc aatcggtggc
     3901 atcaaaacga aatcatattc ctagaaaatc attacactat cgcacaccat tggaagtatt
     3961 tttgagttac gtaggtaaag atgatttgtc tagcttaatt tgacaaacga aaaatttatt
     4021 atatacttga attgttcatt aatgaaagcg aaaccaatcg ttagcaacta tgtaaaagaa
     4081 aggaagattc ttgtgaaaaa aagtattttg cttatttctc atagtcaaaa agtaacagat
     4141 ggtattaaag aaatgattga gcagatgcaa aaatccgaag aggtgcaaat tttttctctc
     4201 ggcggaataa atggcgagat cggatcagac ccgatgaaaa tcgtagaagc agtcaatgaa
     4261 tcaagcgatg cagctagtta ttttgttttt gcggatatcg gtagcgcggt attgaactcg
     4321 gaactggcaa aagacatgtt ggaagaagag cagcagcaac gttatcatct ggtggatgcg
     4381 cctttagtgg aaggagcttt tgcagcagcc atcactgccg gtataacaga tgatactcag
     4441 caaatcttaa cagaagcaca aaatgccgga aagaaaggat gggaataaat tgaaaaaaat
     4501 catgaatgat ccaagcaata ttgtcgaaga aatgctggaa ggattagtaa aaagctatcc
     4561 agaacttgtt catcgtgtag aatcttcccg tgttgtagcc aagaatcaga aagcggaaca
     4621 agttggtttg gtttctggtg gaggaagcgg acatgaacca tcccatgccg ggtttgtagg
     4681 ggaagggatg ttaagtgcag cggtgcttgg tgatgtgttt acttctccaa ctccagatca
     4741 gattcagacg gctattaagg aagccgatag tggaaaagga gttttgctga tcgttaagaa
     4801 ttatacaggg gatgcattga acttcgatat ggcaaaggaa ttagctgcta tggacgatat
     4861 tgaagtagaa agtgtgatcg tagatgatga tatcgccgtt gaaaatagta cgtatactgc
     4921 tggaaaacgc ggagttgcag gtactgtcct tgtacacaaa atcgtgggcg atgcagcaag
     4981 aaacggtgct tccctagctg agttgaaaga actgggagaa aaggtcgtac aggcaactaa
     5041 gacgatcggt ctagcgcttc gagcagcaac tgtaccagaa gtagggaaac caggattcga
     5101 actaggagaa gatgagatcg agtatggtgt tggtatccac ggtgagccag gctatcgtcg
     5161 agaaaagatg cagccttcta aagtactggc aaaggaactt gttactaaga ttttagatga
     5221 ttactcgaat ttaccaaaag aagcaggtgt actagtcaac ggaatgggcg ggacgccttt
     5281 gatggaacag ttcgtgttca tgaatgatgt tttagctctg ttggaagaaa gaggcgtcaa
     5341 tgtagttttc cgaaaagtcg gtaattttat gacctcacta gatatgcaag gtctttctct
     5401 aacactgatc gacttgacgg agacacaatg gaaagattct ttagaaagca atgtacagac
     5461 gatttcatgg taaaaaatct caagataaaa gggtgataat atgaaactaa ccgttcaaga
     5521 aatccaagaa tggctcacgc aatttgcaga ggcaatcaat cagaacaaac aatatctcag
     5581 tgatctagat accccgattg gcgatggaga ccatggaaat aacatgggaa gaggggtttc
     5641 agcttatgaa gaagcttttc aagccgatca tcctgaaaca atcagcgata cattcaaagt
     5701 attctctatg gcgatgatat cgaaagtcgg tggggcttct ggtccgttat acggatctgc
     5761 attcatgaac atgatgaaag cgacaaaaga ggtcgattct attgattctc aagaaaaatt
     5821 aggcgaagtg atcgagcagg gaacagcggg tattcaaagc cgaggaaagg cagaagctga
     5881 cgataaaacg atgttagatg tttggctgcc agtcacagag gcattgaagg cagggaattt
     5941 aacaaaagaa atcattgacc aggcaaaaga acacaccaaa gtcttggtag ctaaaaaagg
     6001 acgagcttcc tatctaggag agcgtgcaat cggacatatc gacccaggag ctgcttctag
     6061 tgccatttta ttttcaacgc ttttagatgt gatcaatcaa ccttaagttc aata
//
LOCUS       DAKBVG010000149         6002 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000149 DAKBVG010000000
VERSION     DAKBVG010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.149"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(153..1247)
                     /locus_tag="OUD09_002072"
     CDS             complement(153..1247)
                     /locus_tag="OUD09_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HCT9693313.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1436..2197
                     /locus_tag="OUD09_002073"
     CDS             1436..2197
                     /locus_tag="OUD09_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCT9693314.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2312..2752)
                     /gene="fabZ"
                     /locus_tag="OUD09_002074"
     CDS             complement(2312..2752)
                     /gene="fabZ"
                     /locus_tag="OUD09_002074"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCT9693315.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            2972..3727
                     /gene="fabI"
                     /locus_tag="OUD09_002075"
     CDS             2972..3727
                     /gene="fabI"
                     /locus_tag="OUD09_002075"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCT9693316.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(3837..4850)
                     /locus_tag="OUD09_002076"
     CDS             complement(3837..4850)
                     /locus_tag="OUD09_002076"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HCT9693317.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            5015..5935
                     /locus_tag="OUD09_002077"
     CDS             5015..5935
                     /locus_tag="OUD09_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HCT9693318.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         1836 a         1193 c         1072 g         1901 t
ORIGIN      
        1 ttacagaaca ccaatcgaaa tatttttgag ctatgtacaa gaagcatttt attctagctt
       61 aatttgacaa atcataacct ataaaaaaac aaaataaaaa agcagaaata actggctatt
      121 tctagcggat tatttctgca ataactaatt atctattttt tattttcaat taatttaaag
      181 cttgaccatg gtttcaaaaa gtcaaggagg aataattcgt catcggagat tcttcctaca
      241 acattgacac gtccgtcgtt tttcatatcc ttcaaggcga tttgtgtttc tcctttgtat
      301 tgaccgtacc cttcattgtc aatcagtaca tccccatgat gcatatctcg cgtattatga
      361 ggcgggaaat ctttatcttt gtagtataca cgagtcatcg ttgaacgaag gatatattct
      421 gagcgatccc ctcgataact atgaaggtta tcaaacaagc agattcgttc gttttctgta
      481 ataccttctt ccgtatctac tttcaatgtt ggataatccg cgttaaatgc ttctgccatt
      541 tctttcaatt cttcttcact tgcgtacgca tttccaatag aaatatcatc aatcaatcct
      601 gtaaggatta aatgcttcac ttgtgtcgca atttcaagat ctcggtggtc ttccagcgtg
      661 cacaagccat cttgcgtagg ccaagggcca aactcagcag attgcgaatt gacgaatgcc
      721 atcgtattca aattatattt tctgaatttt tctgaacaaa aaacaaagtg ttcgtatcct
      781 aaaccagagt atcgatgagg ataaaaatta tgagaaccta ataaattatc cgtgtttggc
      841 gaataactca tgatgttgtc aacgtaattc gtgcccgaac tcatattgat ctcgattttg
      901 ataccaaaag gattgcgcgt catttttgct tcttctgctc ctgtgaatcc gatatctaag
      961 cggaccccgt aggcacccat cttatggaaa aaagataagt catcgtaaga aatctccaac
     1021 tgttcaaata acgctggatt gatatcaacc attacttcca tgcctaggct attcgcgtaa
     1081 tcaactactt ctttaaattc tgaaagaact ttttctttat catcattgat ctgcagcaaa
     1141 ctggtaaaaa cccgtttata cccatactta tgagctaaat ccaagtacgc tttgtccttt
     1201 tcaaatgttg accgttctgg ataaatcgag attcctaatt ttcccatatt accatttcct
     1261 cctgcatctt ttcgtttttg tatccactta cattatagca aacacaaagt aaatggtcta
     1321 caccttttct ttttctttta aaataaatga taaattccaa taatttaagt aggtattttc
     1381 cctctatttt gttatcatta aagataacaa aatagtaaat tggaaaggaa cagttatgaa
     1441 gaaaaataaa gttattttaa gtattagttt agcagcagca ttgctgtttg ctgctggttg
     1501 ttctgcaaac aacacgactt cttctagttc gtctacagaa agttcaaaaa gtacatctac
     1561 tgcggaaagc acgactgcca gtagcgtcga tttgaacgca ttggaattac cacaattgac
     1621 gaaagatgtc gccgaaaatg aagatctggt cgaaatggtg acgaataaag gcacaatcaa
     1681 aatcaaatta tttcccgaac acgcaccaaa agcggttgaa aactttatga cacatgcaaa
     1741 agacggttac tacaacggac tgactttcca tcgcgtcatc aaagatttca tgatccaagg
     1801 tggcgatcct aaaggcgatg gcactggagg ggaaagcatt tggggagaag gatttgaaac
     1861 agagatcaac aaccaccttt ataatataag aggtgcgtta tctatggctc gctcacaaga
     1921 tcctaacagt aacggcagcc agttctttat cgttcaaaat acagatgata tgcatgatgg
     1981 tcttttaaaa gacgactatc ctcaagcaat catcgacgct tataaaaacg gcggttatcc
     2041 ttcactagat ggccattata ctgtcttcgg ccaagtgatt gaaggaatgg atgtcgtgga
     2101 tgctattgca aatatcgaaa cagatagttc agacaaacca aaagaagaag taaaaatcga
     2161 taaaatcaat atactgcaag aagcaaaaca aaaataaaaa aataacgtct gaatcctgca
     2221 tagatgacaa cgtatgctag gattcaggcg tcttcttttt acataaaaag caagtaagct
     2281 tccttttgtt ttgctatatc aggttttata gtcaagcatt ttggttgacg atgaacgtga
     2341 actcgcactc acagactttt ttatcatcta catatgctgc agataacgct gtaccgactg
     2401 atcccttcat tttggtgatt tcaaaatgac atttcagcac atctcctgga acaacttttt
     2461 gacggaattt ggccttattg atcccaccga tataagctct ttttcctaga aactcttctg
     2521 attttaggat aagtatcgaa cctgcttgtg ccagtgtttc tagaatcaac gcacccggca
     2581 tcactggatt gtcaggaaag tgaccttgga aaaaatcttc attgattgtt acattttttg
     2641 tcgcaacgat cttttttcct ggttctaaag tatctacata atcaatataa caaataggat
     2701 aacgattagg aatcaattcc attacttcag tagcagataa tacgtaagac ataattaaac
     2761 tccttctttc aggcaattgc cattttcatt actttgacta tcaaagtatc atattcctaa
     2821 atatttagca atcttttttt aatttaagaa aaaatagttt gacaatcaaa acattttgac
     2881 taataattaa gaaatcaaga agttatttta gctattattc tctatttttt tacgactatc
     2941 aaatttttat cagaaacaaa ggagagtcat tatgtcattt ctagaaggaa aaaaaatcgt
     3001 cattatgggc gtcgctaaca aaaaaagcat tgcttggggt tgtgctgaag caatgttgaa
     3061 acaaggggca gaaattatct atacttacca aaatgaacga atgaaaaaat cattagtcaa
     3121 attggtcggc gaagaagcat tcactgtaga atgtgatgtt tctagcgacg aaagcatcga
     3181 aaaagcaatg aatgccatcg gtgaatatgc tggggagatc cacggccttg tccatgcagt
     3241 tgcttacgcc aacaaagaag aattgtccgg aaatgtgtct gatatttcca gagacggcta
     3301 tcaattagct caagatatca gcagctactc tttaattgct gtatcaaaat acgcacaacc
     3361 gtttcttgca ccaaacagcg ggatcgttag catgacttat ctaggttcag aacgcgcaat
     3421 cccgaattat aacatgatgg gtattgcgaa agcttctctt gaagctgcta tccgttattt
     3481 ggcagctgag ttctcgccta aaggaattcg ggtcaatgga atctcagctg gggcaatcaa
     3541 aacattagct gtcacaggtg ttaaagacta tcaaaaactg atccagttat ctgaatctcg
     3601 tacgcctgat cagcaaggtg tgacgatcga agaagtcggt aatgcttgtg ctttcttaat
     3661 cagcccactt tccagcggaa tcatcggtga tattatctac gttgacaaag gtgtccatct
     3721 ttcataaact tttgttgcat tctttaaagt aaaacatata aaaatcagga aaaaactttt
     3781 taaagtctct tcctgatttt ttctttttac gtttttttca actcattact ttgaaattat
     3841 tccacgactt caatccagcc ttctggtgct tctatgtctc caaattgaat acctgtcaat
     3901 tcatcataca accgttttgt tactggtcct acttccgttt cactataaaa tacatgaaga
     3961 ttgttattgt gataaatccc accgattgga gaaataacag ccgctgtacc acatgcacca
     4021 gcttctgcaa attgatctag atcatcaatg aaaatatctc cttcaattgc ttgcagtcct
     4081 agtcgttctt cagctaactg caacaaggaa tatttcgtga tacttggtaa gatagagggc
     4141 gattgcggag tgatgaattt tccgtctttt gtaattccaa agaaatttgc tgcacctact
     4201 tcttcgattt tcgtatgtgt ggctggatca agataaacac aatctgagta atccagttct
     4261 tttgcttctg ttcctggaag taaactcgct gcgtaatttc cgccgacttt tgctgcacca
     4321 gtccccttag gagcagcacg atcgtaatca gatacaataa aattcgttgg ttttagtcca
     4381 cctttgaaat acgctcctac tggcgtacag aagacgacaa ataaatattc tggagctgga
     4441 tggacaccaa tgttttcacc gaccccaatc agtaaagggc gcaagtataa tgtagcgcct
     4501 gttccatagg gcggcacaaa atcttcattt gcttttacca cttgcttgac agcatcaacg
     4561 aacatctttt cagggaccat tggcatcagc aatcgtctag cactttgatt catccgtcga
     4621 ctattttgtt caggtcgaaa gagattgacc ttgccgtctt ttcgacgata agcttttaga
     4681 ccttcaaaac attgctgacc ataatgaagc gctggagagc cttcatgaat cgtcaggtaa
     4741 ttatcttctg tcaattctcc ttcttcccat tgtccgtctt tccatttggc aataaaacgc
     4801 caaggagttt tgatataaga aaaacctaga ttgttccaat cgatttgcat atacttacca
     4861 ccttcgttat tttctccaca ttttatcatt atccatatta tttgtcattt tattttcagc
     4921 ttttttcctt cattatagtt atttttgcta tttccttttt tgaaaacttc ttggtacaat
     4981 acagaaagaa tcaaatttta gaaaagagga ttttatgttt tcattattag caactgccgg
     5041 tacgacagac tcttctggcg agacattgac agatcaagct gtcagcaaag taaatgcgtt
     5101 tcagcggttt tgggacggga ttgattggga cgccattttg gctgccttta tccaaaaaag
     5161 tttatatctg atttttttga tcgtcctttt ttggattctt caccgtgtgg gaaaatatct
     5221 gattgataag tcttttaatc agtattctaa aaaagccatt ctaaacgaga gccgcttaaa
     5281 gactttacat agtcttttaa atactatttt tcattatacc cttggtttct tttttatcta
     5341 tgcaatttta tctgctatcg gtgtgccgat cggatcactt ttggctggtg ccggaattgc
     5401 aggtgtggca atcggtcttg gtgcccaagg gttcatgagt gatgttatta ccggattttt
     5461 tattatcatg gaacaacaga tggatgttgg ggactacatc aagctagcaa acttaacgat
     5521 tgaaggaacc gtcgtatctg ttggtatccg tacccttcag ctgaaagcaa cggatggtac
     5581 tgtccatttc atcccgaatc gaaacatcac cacgatcagc aatctctctc gtgcaaatat
     5641 gcaagtactt ttagatattc ggattgttcc tgaagaagga tatgacaaaa tctatgagat
     5701 cattgatcga gtaaatcgta cattagctga aaaataccaa gaagagctcc aaactgaacc
     5761 ttctatcttt ggattagtgg atcttggaaa cagcaatttt gcgattcgta cggtctgcta
     5821 cgcattgaac ggaaaacaat atgccttaaa agaagagttc ttgagctcct atgtcaaaga
     5881 attgacagct tctggtttca cgattccgaa cagtccaatt gctgtcggaa aataatttgg
     5941 acaaacgatg caacgaatct cactaaaaag agcgtgggac aaaagtaaag aacacttttg
     6001 tc
//
LOCUS       DAKBVG010000150         5974 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000150 DAKBVG010000000
VERSION     DAKBVG010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5974
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.150"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            347..1036
                     /locus_tag="OUD09_002078"
     CDS             347..1036
                     /locus_tag="OUD09_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCT9693319.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1023..2231
                     /locus_tag="OUD09_002079"
     CDS             1023..2231
                     /locus_tag="OUD09_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCT9693320.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2525..3796
                     /gene="serS"
                     /locus_tag="OUD09_002080"
     CDS             2525..3796
                     /gene="serS"
                     /locus_tag="OUD09_002080"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCT9693321.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4345..5829)
                     /gene="guaB"
                     /locus_tag="OUD09_002081"
     CDS             complement(4345..5829)
                     /gene="guaB"
                     /locus_tag="OUD09_002081"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCT9693322.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
BASE COUNT         1831 a         1127 c         1160 g         1856 t
ORIGIN      
        1 aacatgtcaa caacttattt tttattattt aataagttat tggtgttctt ttgaacaaat
       61 aataatatat caacatcaca tgtgaaagtc aataagaaaa tgcttattta tcaacatttc
      121 tagcttacat gatcattggt tagttctggt cctttttcgt tcgtctttat aactccgaga
      181 gacgtaaaat ccttttgttt tcagtttaat gttcatgttc ttgtcttatt cacaccaact
      241 agatgtctca ccttctaaaa ttttccttaa caaagtgctg cttttatcta tttctgcttt
      301 tttttgctag aataatagta acaatatgac tctggaggat ttatttatga aaattttgat
      361 tgctgacgat gataaagaaa tcgttgaatt attaagtatc tatgttcaca atgaggggta
      421 tgaagcagta aaggcttatg atgggaaaga agcattatct aaaatcagaa caatccctga
      481 tattgaatta cttattttag atatcatgat gcctgagatg gatgggatgc aagtagtgaa
      541 agagctacga aaagaatccc aaataccaat cattatgctg acagcaaaaa ctactgatat
      601 ggataagatc aaaggactgg tcgctggtgc agatgattat gtgaccaagc catttaatcc
      661 gttagaagta atggctagag taaagtcttt attacggcgt acaaaaatgc agattgcccc
      721 agatcagcct gatgtattag aagtaaatgc attaatcatc aataaagact ctcatgaagt
      781 taaaacaatc gatgggaaag aaatccagtt aacagctttg gaattcggta tcctctactt
      841 gcttgctagt catcctaatc gagtatttag cgcggatgaa atttttgagc gtgtttggaa
      901 acaagaaagt attgtctctg ctaaaactgt catggttcac gttagtcatt taagagataa
      961 gattgaagaa gcaactggtg gtgaaaaagt cattcaaacc gtttggggag tgggctataa
     1021 aattgacgca agatagtaaa cgagcaactg aaaaacgccg tcggatcaat ttgacttcaa
     1081 aagaaatcag cgaattgtta gcggaaggaa tcatcacgat tatccttttg cttctattaa
     1141 atgtttctat tctggttgtt atcagttcgg tgatcaacag ttcaccttct ttgacgaatg
     1201 cgatttggga ttcaaagaat atttttgcag aacgattgaa cactgatttg ttctggaacg
     1261 gccggaattt tattattccc tttttctttc tattagatat tggtgttttg tattggcgct
     1321 tgattcgaag atatcgccaa atgcaattgc ggcatatcat cagcgaactt cactacatcg
     1381 caaacggaaa ttatgatcat cgtatccctt ttgaactgag tggtgactta agccgagtgg
     1441 taacaagtat caatggattg gtggatagta ccgtagcagc gattgaagac gaacgtaaaa
     1501 tcgaaaaatc aaaagatgaa ttgatcacga atgtcagtca tgatatccgt accccactca
     1561 cgtccatcat tggataccta ggactgatag aagatggcca gtatcactca gaagaagatc
     1621 ttttaaagta tacgcatact gcttatatca aagcaaagca gatgaaatct ttagtggacg
     1681 acttatttga atacactaag gtacgccagc ctgctgtccc ggtgaatttt tctgcctttg
     1741 acatgatcca gttgatcgaa cagttagcag ctgattttga gttggaagct tctaaaaaaa
     1801 atatccaaat acttgttcaa tcaaaagtag actcccttat catggatggc gataccgaaa
     1861 aacttgtacg tgtcttcaat aatcttttga cgaatgcgtt gaaatacgga aaaggagcaa
     1921 cgaagatcgt aatcgaagta gaacgtatcg gttcagaagt tgttgcaaca gtcaaaaata
     1981 acggagcaat gatcccacaa caggcaatcg ataatctatt cgaccgtttt tatcgtgtag
     2041 aagaatctcg ttcacaagca actggaggaa ctggacttgg tttagcaatc gctcaaagca
     2101 ttgtggcttt gcacggcgga tacatttatg caaaatcaga taagcaatgg acttctttca
     2161 tcatgcacct tccaatcaag aaaaatgaaa aattacctat tgatacacag gaagttattg
     2221 acgaaagctg aaatattcgc tattcttatt acaattatta gaataaatga aaaacacaga
     2281 acccagtaca ctttattctt tttcagagag ctgatggcag gtgtaaatca gcaaaggata
     2341 atttggaagt tccagttcgt ttacgtgccc tttcgaaata agaaaggtcg gttcaacagc
     2401 cgttatgtgt tcaacaagtg cagctgacgc tgaacttggg tggtaccgcg aatttcagac
     2461 tgattcgtcc caagaataat gggatggatc agtctttttt tattttcaaa aaggaggaaa
     2521 tcatatgtta gatgtaaaaa tgatgcgaca aaatattgaa gaagtacaag aaaaattgat
     2581 gactcggggc gtaaaaaaag aaacattagc tgaattcatg gaactagatg aaaatcgtcg
     2641 taagctacta gtcaaaagtg aagaattaaa aaaatatcga aatgatgttt ctgctgagat
     2701 cgcccaattg aagcgtgcaa aagaagatgc ttctgctaaa atcgctgaaa tgaaagaagt
     2761 cggtggcaac atcaaagcat tggatactga aattgctgaa gtggatgaaa aattgaaaca
     2821 aattgcaact actttgccaa accttcccca tgattctgtg ccagttggaa cagatgaaga
     2881 ggacaatgta gaaatccgtc gttggagcga accacgtaca tttgcttttg aaccaaaacc
     2941 acactgggaa attgcagaaa atcttgatat tttagatttt gaacgtggcg caaaagtagc
     3001 cggtagccgt tttgtctatt acaaaggatt aggtgctcga ttagaacgtg ctgtctacaa
     3061 cttcatgctt gatcagcata tttacgaaca cggctatact gaaatgatca ctccttatat
     3121 cgtcaacagt aaagcgatgt ttggtacggg acaatttcca aaattcaaag aagatgtttt
     3181 ccaattggag aatactgatc taacattaat cccaacagct gaagtaccat taactaacta
     3241 ttataatgat gaaattttag atggtgcaga tttacctatt tatttcactg cactaagccc
     3301 tgcttttcgt tctgaagccg gaagtgctgg tcgggataca cgaggactga ttcgtcttca
     3361 ccaattcaat aaagtagaaa tggtcaaatt ttcggatgta gagcattctt acgaagaatt
     3421 ggaaaaaatg acagctgatg cagaagatat cttgaaaaaa ttgcatttgc cttatcgtgt
     3481 gatggcctta tcaactggag acatgggctt ttctgcagct aaaacttatg acttagaagt
     3541 atggatacct gcacaagata cttatcgtga aatcagttct tgttctaact gtgaagattt
     3601 ccaagcacgc cgcgcaatga tccgttatcg cgataatgat ggaaaaatcc actatactca
     3661 tactctgaat ggttcaggac ttgcagttgg ccgtacagta gctgccattt tggaaaacta
     3721 ccaaaatgaa gatggttctg tcacagttcc agaagcgctc gttccttata tggggaatct
     3781 gaaagttatt aaataaagag gctgtgaaaa agccgcttag tttcgagtat taagaaaaat
     3841 tttgtgaaaa tggctttcca catttttgca taaattggac ttaataccga gaaactggct
     3901 tttgaacacc atttatcgag aggttgtgaa atcagcgaat taacctgagt attagagaaa
     3961 aaaatggaaa aatagtccct catattttac cgtttgttga actaataccg agggtcagct
     4021 gattgaacac cgtttagtgg agaggtcgta agcctacccc ccagctccta gttacaagga
     4081 caaattcaaa aaacagctct tcatatttta tgaatgttgt acttgtgatt cggaggtggc
     4141 aagggaacac tgtttattca aaaagaggct gggacagaag cgtttaactc caagaaataa
     4201 gaagaaattc acgaaaattg cttttcaaat ttttgtgaat ttcttcttat ttccgaagga
     4261 gttgcttcta cttccgccgt ttatacgtgt ttagagcgtg agacaaaagt gttctttact
     4321 tttgtcccac gctctttaat gtttttaaga ttcaactgaa tagtttggtg cttcttttgt
     4381 gatttgtaca tcatgagggt gtgattcttt taatccactg ccgctcattt ggataaattg
     4441 cgcttcatca cgtaattgct gtagattggc tgccccaaca taacccattc ctgaacgcaa
     4501 accgccgatc atttggaaaa taatatctgc aacacttcct ttataagcaa cccgaccttc
     4561 gattccttct ggtactaatt tatttgcttc attgacacta ccttggaagt aacggtcttt
     4621 tgaacctttt tccatcgcgc ctaatgagcc catacctcgg tatgttttga atcgacgtcc
     4681 ttggaagatt tcgaattcac ctggtgattc atctgtccct gccaacatac ttcctagcat
     4741 cactgcatgt ccgccggcag ctaatgcttt gacgatgtca ccagaatatt tgatccctcc
     4801 gtctgcaata atcgctttgc catattgacg agcaactgat gctgcatcat aaatagctgt
     4861 gagctgaggt acacctacac cggctaccac acgtgtcgta caaatcgatc ctggtccgat
     4921 accaactttt acgacatcta cacctgcatc atataaagct tttgtcgctt cagccgtcgc
     4981 aacgtttcct gcaatcaatg ttgcatctgc aaaagttgaa cggatttctt gaatttttcg
     5041 gatcacacct gcactatgtc catgagctgt atcgatgatg attgcatctg cccctgcttc
     5101 taataaagca ttcgcacgct caaatgtatc gcttgttaca ccaacggctg ctgctacaag
     5161 caatcgacca tgttcatctt ttgctgcatt tggaaattcg atcactttct cgatgtcttt
     5221 gatcgtgatc aatccgctta aacgaccttc gttatctacg attggtaatt tttcgatttt
     5281 atgtttttgc aaaatctttt ctgcatcttt tagagaggta cctactgggg cagtcactaa
     5341 gttgtctttt gtcatcacat cagcaatcgg catgtgataa tctgttacaa aacgcatgtc
     5401 gcggtttgta attatcccta ccaattttct gttttcaagt gtttcaacga ttggcacacc
     5461 actgatacga tatttgctca tcaattcttc tgcgtctgct acaagatgct gtggcgttaa
     5521 gaagaacgga tcaatgatta cgccactttc agaacgtttg acttttctta cttcatccgc
     5581 ttgctggctg atcgtcatgt ttttatggat cacacctagt ccgccttgtc ttgccatagc
     5641 aatggccatc ttgctgtcgg taacagtatc catacttgca ctgatgattg gaatattcaa
     5701 cttaatattt ttagctaatt gtacgctcat atccacttcg tttggtaaca cgtgactttc
     5761 tgctggtatc aataagacat catcaaaagt aaatcctttt ttcgtgaact ttgtttccca
     5821 gttggacatt tttagtacca ctcctctaat ttagtttatc tattaaaata attgattttt
     5881 tcatttaagt caatgatcta cttggaatca agttagcttt ctcatagaat accaatataa
     5941 agcgtttaca tctgtgtata ttaaaagaga ttgt
//
LOCUS       DAKBVG010000151         5954 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000151 DAKBVG010000000
VERSION     DAKBVG010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.151"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            <1..813
                     /locus_tag="OUD09_002082"
     CDS             <1..813
                     /locus_tag="OUD09_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HCT9693323.1"
                     /translation="KKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSD
                     NGQIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSG
                     DNFIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIES
                     KSLNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHAL
                     ALFGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            807..1229
                     /locus_tag="OUD09_002083"
     CDS             807..1229
                     /locus_tag="OUD09_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693324.1"
                     /translation="MVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            2399..3367
                     /locus_tag="OUD09_002084"
     CDS             2399..3367
                     /locus_tag="OUD09_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCT9693325.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            3384..3908
                     /locus_tag="OUD09_002085"
     CDS             3384..3908
                     /locus_tag="OUD09_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HCT9693326.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINE
                     TGQKAIKSMFFNENENSNQQLVGEKTKTENHKNANQVPELEVLSLLEQQLNQKDMQLQ
                     EKDEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIE
                     KQSKAEKKWWQFWQ"
     gene            4284..4565
                     /locus_tag="OUD09_002086"
     CDS             4284..4565
                     /locus_tag="OUD09_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693327.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
     gene            4567..4983
                     /locus_tag="OUD09_002087"
     CDS             4567..4983
                     /locus_tag="OUD09_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693328.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
     gene            5613..>5954
                     /locus_tag="OUD09_002088"
     CDS             5613..>5954
                     /locus_tag="OUD09_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HCT9693329.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTV"
BASE COUNT         2259 a         1019 c          962 g         1714 t
ORIGIN      
        1 aaaaaaaata caaatggaga atattcagga agtatctcac aatttttagc aaaagaactt
       61 caaaatctag tagatagtaa tataacagat acaaccttat tttttcaaga taatagcatt
      121 tattattccg ataatggaca aattaaacta ctttggaaat cacctttaag tacagaaaaa
      181 aatggtgttt cttcctttac agaaaaaaat gatcttatat ccgtaaatat aaaaaataaa
      241 attaaaaatt tctctaaaaa acaaaatttt cctgcggtta aacgtgtaaa aagtgtctcc
      301 tccggagata actttattat gattaattgg cgtatagcag aaacccaaac aagttttccg
      361 tggtgtgctg cttacgtaga agctgctatc ttatgtaaca aaacagatgt acgtgctact
      421 tcagctagag ccattatgca ttatacctat cctaatttaa acaacagtca aattgaatca
      481 aaaagtctta atcaagatca aataataaaa tatgcaaatt actgtggtgt ttatcctaaa
      541 aaagtagcac gaatactaaa cctttcagag gtccaaacac aactaagaaa aggaaatgcc
      601 atttatatgg gaactgctgg ttatggcaat tatagtaaca gccgtcatgc acttgcatta
      661 tttgggtggg tcaatcaagg tggttatcaa acttactatg tgtggaatcc ttggtataac
      721 tacccaactg taataagtgc aaatgcagga ccagttacaa ttcctgtacc tggaggtggt
      781 tttacttggg acaataccat tacaaattgg taaataaacc cattgcttta gttatgggtc
      841 tagtgttagt tcttttctta actagttgtt caaaaaaaca agaaatacaa aagactgatc
      901 tgattccttt tctaggaact tggcaaacta aagatcattc agaacgtcta gtaatcacta
      961 atttaaatac ttcaaaaaat atagaacaat ataccatctt agtaaaagag aacaatcata
     1021 aaattcaaat cattgataaa aatttaaata taaatgaaca agttttcatt tacaaagcaa
     1081 caaataattt actaacactg cgctctaata aaaaacaatt agattacact tttagtttgg
     1141 attctccaaa aaagctcgta tttattttga caacgaatga tgggacagaa gggacagcta
     1201 aaccaattga attttttaaa gctgattgat gaatagttgt ttgatattac aaaacaataa
     1261 tttcaccgaa ggtgaaacaa gcgaaacttt acaaaaagtg gctagcattt gctagccttt
     1321 tttgtattgt ggagcgtggt ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1381 aagcggttat ttgcaccaaa tacgtaggta tttggctagc aaatatagag cttgccaaac
     1441 actttagtga gttttgtaga aagtaccttt aaaggtactt tcacattacg aacgatacgt
     1501 ccgtaggacg tactgaaagg gtgggattta tgagcgaact aatgcaaaat ttggctagca
     1561 aaaaaagtta aaaaatatat gctaataatc gagcgaaatg aacacttttt caagtgagcg
     1621 aatccgccat gacaaagctg aagcagttta tttcagcttt gtcatggcgt gtgcctagac
     1681 ttttaaaaaa gtcggcacac gagcaaatat ctttatcaag ctctaatttg cgttttaaga
     1741 cgtttgaaac gtctaagtat ataaatatat aagagtaggt ttaagagtgc ttaaatcgaa
     1801 ttttagaggg cattcagaca actttagggt tgttagggct tgccctgacc gcctgtaagg
     1861 cgctttaaga acaagataag ggatttatac taagactgtc ttaaaacgtc tcaaacgaag
     1921 gctcatagct tctcaaagag ttccttatga acatttttag caaaggagcc ggaagcgact
     1981 gcgcagtata atgtgagagc agagcgatca caccttttta agcgactgta gggagtaaga
     2041 ttcccagttg agaaaaatgc gcgaatctgt ttggagaaaa aagaagtggt tttttaggcg
     2101 ggaaattttg tccccctctt ttataatctc tttttaaacc tctttttaaa cctctttacc
     2161 gtcggctata tccttactct cccaagggaa tgacgctttt atctacatcg ttttgtctgt
     2221 ttatctacat cgttttgtct gtttatctac atcgttttgt ctgtttatct acatcgtttt
     2281 gtctgtttgt acattgaatt agtacatcga cgatgttaat ataagatgta gataaacagg
     2341 cattttgtat ttcataacgt gtatctcttg tgcgagtaag aaaggagcta ttttaaatat
     2401 gtctaatgaa atcgtaaagt atcatcatga attaaataca attccgttgc gaaaatttac
     2461 accagtcgaa atgaatttgt tcttttctat cgtatcaaga atgcgtgatg taggtgataa
     2521 aaaagtacaa tttacattcg agcaattaaa agatttaagc aattataaag caactgcaaa
     2581 tgttcgattc atagatgatt tagaaacaac gtatgacaaa ttaatggatt tacgttttgg
     2641 tagaagaagt gctgacggtt tgcaacgtga acgttttgtt ttatttaatc aatttaaaat
     2701 cgacggaaaa tctgatattc cttttgctga aatacaagtt catgaaaaag ccttaccctt
     2761 acttaataat cttgaagaat gggtgcgcta ttctctccaa caattcaatg aattagaaag
     2821 cagttattca aaaacaatgt ttcgattact aaaacaatac agaaccaaag gtttcgctta
     2881 tttttcaaaa gaagactttc atgaattact tgatattcct aaaagttaca accaaggtac
     2941 gattgatcaa cgtgtgttca atccaatacg acaagaacta accgcaatct ttaaaggttt
     3001 aacaatcaaa aagaaatacg gaaaaggacg gggaaagcct gtcattggct atcaatttac
     3061 atttaaacct gagataaaaa aatcagacga ctttaacaaa catgccccaa caaaagaaga
     3121 acaaaacaaa gcattagaac tagctaacca acaagttcaa tctgaaaaag cacctacttc
     3181 attacaagaa aaaatcaaag aacaaaagcg aaaattggaa cttttacaac agttagaaaa
     3241 gttagaaaga gaacaaaata cccaaaataa agaaaataat gcacaaaaat cagagataaa
     3301 ttccgatatt ccttcagaac taaaaaaaga cttattagac aatcttcaaa acttattcaa
     3361 agattgaggg gtttttagat tttatgacag aaaaaacaat caaagaactt gctgaagaat
     3421 taggagttag taaaacagca attaacaaaa aagtttctga cgaaaatcga aaactttggt
     3481 tttcgaaaat cggaaacaag tttgtgatta atgaaaccgg acaaaaggct ataaaatcaa
     3541 tgttttttaa cgaaaacgaa aactcaaatc aacaactagt tggcgaaaaa acgaaaaccg
     3601 aaaaccacaa aaacgccaac caagttcccg aattagaagt cctttctctc ctagaacaac
     3661 agttaaatca aaaagatatg caactacaag aaaaagacga gcaaataaaa caaatgcaaa
     3721 aattactcga ccaccaacaa caactcaccc tgcaagccaa ccaacaaatc gagaaactgc
     3781 aggaacaact acagttgact tatacggaag aatcttcgga aaataaatca acgactcttt
     3841 ctgaagaaac agaatcgata gaaaaacaat caaaggcgga gaaaaaatgg tggcaattct
     3901 ggcaataaaa ccagcaattt aaattaataa aaattgctgg ttttattctt ctttttgctt
     3961 ttgcgagcag agcaacatgg acccatgttg cgatattttg gtggcgcccc aatccccgtt
     4021 ttttcgttga atcttactcg gactgcactc gttttacagc tgatttttct gaattttgaa
     4081 agagcataag gaaacgtggt atcactttct tacgaaaggt gctagatagg ctgtgcctat
     4141 cacgtttctc agaaaaaaat ttgctatcac ttttttgcca ttgtggctag gctatgtata
     4201 gtgttttggc gaaaaatagc tatacaaata ctatatttgt atggtataca taaattatac
     4261 gctgatacga aaggaaaatt tccatggaca ttttagttcg aaatatcaat cccaaaatta
     4321 ttgcaagaat agataaacgt tgtcaggaac tcacaaccaa aacgggcaaa aaatggagtc
     4381 gaaataaata tctaaaagtt ctaatcgaaa gtgattttga ccgtcaactt atgaattaca
     4441 aaaaagatca attcgattta ttactcgaaa aatttattgc cattcaaaca gaaaatacga
     4501 acatactgaa tgactatgtt cacacaaaca accaactcat tgaaacactt atcggaggaa
     4561 tataacatga gaaaaacttc tgttcaaaaa cacgtaaccc tttatcccag tcacgttgac
     4621 gttttagatt ccatcatcga aaaaaatcca gacgtcaaca attattcaga agccattcgt
     4681 tttttatgta tgagttatga acctgatacc aatacaacca caaccagtaa ccaagcaaaa
     4741 ctcaatgcca tgggaaaaga aatttctatc atgacagaaa tcatggcaga atttgctgat
     4801 ttatatctaa aagatactgg tgttcttatc ggtacaaatt ctaaagtgta tcaagaagtg
     4861 aaagaaaaaa ttgaaaatca actcaatcct tcccgacgaa atcaacccaa aaatcgtcaa
     4921 aaagaaacca atcaaacctt tacttctgga aattttgacc caacaaactt tctaaaaaga
     4981 taaaaaaaga aaccgatccc taaaaaaatt cctcgatttc tttttaggga tcagtcttcc
     5041 agcaagattt gcaagttggt accaacttgc gaaaaaaaag aaaaatccgc ttatgcaact
     5101 tcttcttttt gtattttgag gcgctacccc aatccccaag tatcggttat ttaatagcgc
     5161 ggaaatccaa agaataggac gtaccctgaa acaaaaaaag actgctctca cgagcagccc
     5221 aaaatgagat tatcctattc atcaacataa aaatattaca aagtgaagaa ctagagaaag
     5281 acaaaaaaag catatcaaat cctctcaaaa ataacaagtc tttaaaaaat gacagataaa
     5341 aatgcacaaa gaactaaaaa agagaaatcg cgtaaaatca agcattagaa gctagtaact
     5401 agctagacca attaaaaatc acaaagtgag cgtctctatc cattggtaga ggggctactt
     5461 ggcatagtcg acattatgtt aactagctcg ataaataacg attatgttcc caaaacaata
     5521 ttttatgaac tataacaaaa atttgttatc gcatattgta tattttaatt atataattat
     5581 aggtgtatac cacttaaatt taacacaaag acgtgattat tttgaaaccc aaaaagaaaa
     5641 tttatctagg tctcctcttt tccttgatga ttggaatttt tagtatagga attacaaata
     5701 cctatgcttc agaaaattct aatttcaata tcaactccca accaattgaa gaacaagcaa
     5761 ttaattttgc gaaaactgat tttgaacact ccataacttc aattctttta atggaaaata
     5821 acaccgaaac tgatattaac tatactcttg gttcgccatt taaaatgctc gatggagaaa
     5881 atacggcaaa tagttctatt ttctttccag tattttcaga cgctgataat acgattaaat
     5941 atatttatac cgtc
//
LOCUS       DAKBVG010000152         5808 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000152 DAKBVG010000000
VERSION     DAKBVG010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.152"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            151..1095
                     /gene="mvk"
                     /locus_tag="OUD09_002089"
     CDS             151..1095
                     /gene="mvk"
                     /locus_tag="OUD09_002089"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HCT9693330.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            1088..2065
                     /gene="mvaD"
                     /locus_tag="OUD09_002090"
     CDS             1088..2065
                     /gene="mvaD"
                     /locus_tag="OUD09_002090"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HCT9693331.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKKGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            2078..3163
                     /locus_tag="OUD09_002091"
     CDS             2078..3163
                     /locus_tag="OUD09_002091"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HCT9693332.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            3160..4224
                     /locus_tag="OUD09_002092"
     CDS             3160..4224
                     /locus_tag="OUD09_002092"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCT9693333.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKDPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAQEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(4473..5276)
                     /locus_tag="OUD09_002093"
     CDS             complement(4473..5276)
                     /locus_tag="OUD09_002093"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HCT9693334.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIRDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(5296..5601)
                     /gene="kduI"
                     /locus_tag="OUD09_002094"
                     /pseudo
     CDS             complement(5296..5601)
                     /gene="kduI"
                     /locus_tag="OUD09_002094"
                     /inference="COORDINATES: protein motif:HMM:NF002091.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
     gene            complement(5583..>5808)
                     /locus_tag="OUD09_002095"
     CDS             complement(5583..>5808)
                     /locus_tag="OUD09_002095"
                     /inference="COORDINATES: protein motif:HMM:NF018513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HCT9693335.1"
                     /translation="LWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKIIKEAIPSLLLI
                     VIIIGGILTGIFTAVEASAIAVIYKWVIRF"
BASE COUNT         1748 a         1241 c         1203 g         1616 t
ORIGIN      
        1 agttttactt ctgagatggt tttagtaaaa taaaggagga atgttttttg agacccttag
       61 tggacaaaaa aattcgtgaa gcagcgcaac tcgttttggg cgctttattt tttgcttaaa
      121 aatcactttt acgtgagaag gagttagatt atggcaaact atggccaagg agaatcaagc
      181 ggaaagatca tattgatggg cgagcacgcg gttgtttatg gagaaccagc gattgccttt
      241 cctttctatg caacaaaagt caccgcattc cttgaagagc tggatgcaat ggacgatcaa
      301 ctggtttctt cctactattc aggaaattta gccgaagctc ctcatgcatt aaaaaatatc
      361 aaaaaattat tcattcactt aaaaaaacag catgacatcc aaaaaaactt gcaactgacc
      421 attgaaagca cgattcctgc tgaacgtgga atgggatcaa gcgctgcagt cgccacagca
      481 gtcactcgtg ctttttatga ttacttagca tttcctttgt ctcgtgaaat actattagaa
      541 aatgtccagc tttcggaaaa aatcgcccac ggtaatccta gtggaatcga tgcagccgct
      601 actagcagct tgcagccgat ttattttaca aaagggcatc cttttgacta cttttctttg
      661 aacatcgatg cttttttgat tgtcgctgat acaggaatca aaggacaaac aagagaagcc
      721 gtcaaagatg tcgctcacct ctttgaaacc cagcctcatg aaactggaca aatgatccaa
      781 aaattaggat acttgacgaa gcaagcaaaa caagcaatca ttgagaattc accagaaacg
      841 ttggcacaga caatggatga atcacagtca cttctggaaa agctgacaat cagcaatgat
      901 tttcttaatc tattgatcca aacagcaaaa gataccgggg cattgggtgc caaattaact
      961 ggcggtggac gcggtggctg tatgattgca ttagcacaaa caaaaacaaa agcccaagaa
     1021 atcagtcaag cacttgaaga tgcgggtgct gctgaaactt ggatacaagg attaggagta
     1081 catacctatg tttaaaggca aagcacgcgc atataccaac attgctctaa tcaaatattg
     1141 gggtaagaaa aatgaagaac tcatccttcc aatgaacaac agcctttcgt taacattgga
     1201 tgcattttat acagagacag aagtcatctt ttctgatagc tatatggtag atgaatttta
     1261 tctagatggc accttacaag acgaaaaagc aacaaaaaaa gtcagtcagt ttcttgacct
     1321 ttttcgtaaa gaagccggcc tctcactaaa agcttcagta atcagtcaga atttcgttcc
     1381 taccgcagct ggattagcct cctctgccag cgggctagct gctttagcag gagcttgcaa
     1441 tactgctctt aagcttggat tagacgatct ctctctttca agatttgctc gacgcgggtc
     1501 tggttcagct tgccgaagta ttttcggtgg tttcgttgaa tgggaaaaag gccatgacga
     1561 cttaagttct tacgctaagc cagtcccttc cgattctttc gaagacgatt tagcaatggt
     1621 tttcgttttg atcaacgacc agaaaaaaga agtgtccagc agaaatggga tgcgtcgaac
     1681 agtcgaaaca tccagttttt atcaaggctg gttagattcc gttgaagggg atctatatca
     1741 attgaaacaa gcaatcaaaa caaaagattt ccaacttctc ggagaaacga tggaaagaaa
     1801 cggactaaaa atgcatggaa cgactttagc tgcccagcct ccattcactt actggtctcc
     1861 tgacagctta aaagcgatgg atgcggtgcg gcaattgaga aaaaaaggca ttccatgcta
     1921 ctttacgatg gatgctggcc ctaatgtcaa agttttagtg gaaaacagcc atttatctga
     1981 ggtacaagaa acatttacca aattatttag caaagaacaa gtaattactg cccatgctgg
     2041 tccaggaatt gcgattattg aataaaggga gatttttatg attgaagtat ctgcaccagg
     2101 caaactctat attgccggag aatatgcagt tgttgaaaca ggacatccag cagttatcgc
     2161 tgcagtcgat caattcgtaa cagtcactgt agaatccgca cgaaaagtcg gaagtatcca
     2221 atctgcacaa tatagtggga tgcctgtacg ttggacaaga cgcaacggag aattagtttt
     2281 agatattcgg gaaaatcctt ttcattatat ccttgctgct attcgtttga ctgaaaagta
     2341 tgcacaagaa aaaaacattc ttttatcctt ttatgatctg aaagtaacaa gtgagttaga
     2401 cagttcaaat ggccggaaat atggattggg ttctagcggt gccgtgaccg ttgcaacggt
     2461 caaagccttg aatgtttttt acgcgttgaa tttatctcaa ttggagattt tcaagattgc
     2521 agcactagcc aatttagcag ttcaagataa tggttcctgc ggcgacatcg ctgctagctg
     2581 ttatggtggc tggatcgctt tctcaacctt cgaccatcct tggctccaag aacaagaaac
     2641 tcagcattct atcagtgagt tacttgccct ggattggcca ggtctatcca ttgagccatt
     2701 gattgctcct gaagatttac gtttattgat tggttggacg ggtagccctg cctctacttc
     2761 tgatttggtc gatcaagttc accgttcgag agaagacaaa atggtggctt atcagctttt
     2821 cttaaaaaac agtacagaat gtgtcaacga aatgatcaaa gggtttaaag aaaataatgt
     2881 aacgttgatc caacagatga ttcgaaaaaa ccgacaatta ctgcatgatt tatctgcaat
     2941 cactggggtc gtcatcgaaa cgcctgcttt gaacaaattg tgtaatttag ctgaacagta
     3001 tgaaggagcc gcaaaatctt ctggtgcagg tggtggcgat tgcggaatcg taattgttga
     3061 ccagaaatct ggcatccttc ctttaatgag tgcatgggaa aaagcagaaa tcactccact
     3121 gccgttacat gtctatagcg atcaaagaaa ggaaaaccga tgaatcgaaa agacgaacat
     3181 gtttcactag ctaaagcctt tcatgataaa caaaaaaacg aatttgactt cgtacggatc
     3241 atccataacc ctttgccgca aatagcagtt tctgatgtcg atctaagtac acaggcagtt
     3301 gggtttacac taagcagccc attttatatc aatgcgatga ctggcggaag cgaaaaaaca
     3361 aaaaaaatca accaagatct tgcaatcgtc gcaagagaag cagatttgat gattgccact
     3421 ggttcagtca gcgctgcctt gaaagaccct tctttagccg atacttatac gattatgcga
     3481 caagaatatc ctcacgggaa aatcattgcc aacatcggtg cagggacttc tgtcgaaaga
     3541 gcccaagaag ctatccgatt gtttcacgca gatgccttac agattcattt gaatgcacct
     3601 caagagttgg ttatgcctga gggagaccgt gattttacca attggaaagt acttattcaa
     3661 gagactcaaa cagccattga cgtccccctc atcgtaaaag aagtcggttt tggcatgaca
     3721 agagaaacgc tcaatgatct agctgcttta ggagttcata cagttgatat cagcggtcga
     3781 agcggaacaa gcttcaccca gatcgaaaac gcccgtcgct ccaagcggga actgagttat
     3841 ttagctgact gggggcaatc gacagtctct tcattgcttg aagcaaatga agcagacact
     3901 tctatggaaa tcctagcctc cggaggtatc cgcaatgcct atgacatatt taaagccctt
     3961 tgtctcggcg caaatgcagt gggtacttca ggaaccgtac tgacgcatct gatgaatcac
     4021 ggcgtagaag aaactattat actgatgaag caatggcaag aagagcttcg tctgctttat
     4081 actatggtcg gcgcaacgaa taccgcggcc ctacaccaac aatcactgat cttttccgga
     4141 ccagtgaagg attggtgcga agcaagaggg atcgacttag tgaagtatgg aagacgcaca
     4201 gaaaagagcg tgggacaaaa gtaaagaaca cttttatccc acgctctaaa cacgtataaa
     4261 cggcggaagc aaaagcaact ccttcggaaa taagctgaaa ttcacaaaaa tttgaaaagc
     4321 aattttcgtg aattccttct tatttcttgg agttaaacgc ttctgtccca gcctcttttt
     4381 tcgaataaat gatgtttaat cagctgtcct tcggtattcg ttcaagccgc tgatttcaca
     4441 acctctccac taaacggtgt actaaagctc ttttaaggtt gttttccgat ataggcaagt
     4501 atgcctccgt ccacatacaa gacatggccg ttaacgaaat cagatgcgct agaagccaag
     4561 aagacagatg gtccagccaa atcgattggc tcgccccagc gtgctgctgg cgtacgtcca
     4621 acgataaatt gatcgaatgg atgacgttca ccgttttctt gtgtttcgcg taatggagca
     4681 gtttgcggtg tagcaatata acctggtcca attccattac attggatatt gtattgaccg
     4741 tattcagaag cgatgttttt cgtcagcatt tttaatccgc cttttgccgc agcataagca
     4801 ctgaccgttt cgcgtcctaa ttcactcatc atggagcaga tattgatgat tttaccgccg
     4861 cctttttcga tcatatcccg aataactgct tttgaaacga tgaatggggc atttaaatcc
     4921 acgtcgatta cttggcggaa gtctgccgca tccatttcga tcatcggtgt ccgtttgatg
     4981 atccctgcat tgttgactag aatatcaact gaacctacat cttctttgat ctgttgaacc
     5041 atcgcttgaa ccgcttcttc atcggtcaca tcgcagacat aaccttttgc atcgatgcct
     5101 gcttctttat aatgcgctat accttcatca actgattctt gattcaagtt attaaatacg
     5161 atttttgctc ctgctgtagc taaagagcgt gcaatctcaa atccaatacc atagacagca
     5221 ccggtgacta atgctacttt tccatctaaa cgaaacatat ccatactaaa ttccatcatt
     5281 ttctctccct tatgcttatt ttaattgatc catttgcacc atatccatgt ctgtataggt
     5341 gatattttct ccgcacattg cccagatgaa tgagtaattg cttgtaccta cacctgaatg
     5401 gatcgaccag cttggcgaaa taatggcttg ttcattgtct acgactaaat gttttgtttc
     5461 atctggtttg cccatcatat ggaacacacg tgtatcttca ttggcataat caaaatagac
     5521 ataagcttcc atacgacgtt catgtgtatg acaaggcatc gtattccaag cacttcctgg
     5581 ttctaaaatc gtataaccca tttataaatg acagctatcg ctgaagcttc tactgctgta
     5641 aatatccccg tcaagatacc accgataatg atgacaatca atagaaggct aggaatcgcc
     5701 tcttttatta tttttccagc atgagccatc tgtccttttt cgacaacagg gtatttattc
     5761 tttttagcaa cgatgaatgc tactagcatg attcctagcg cccacaaa
//
LOCUS       DAKBVG010000153         5780 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000153 DAKBVG010000000
VERSION     DAKBVG010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.153"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(<1..147)
                     /locus_tag="OUD09_002096"
     CDS             complement(<1..147)
                     /locus_tag="OUD09_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCT9693336.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWAEQGLIRSSRQEENDYRLFD
                     IDDIF"
     gene            247..1968
                     /locus_tag="OUD09_002097"
     CDS             247..1968
                     /locus_tag="OUD09_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT9693337.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            1983..3767
                     /locus_tag="OUD09_002098"
     CDS             1983..3767
                     /locus_tag="OUD09_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT9693338.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(4148..5701)
                     /locus_tag="OUD09_002099"
     CDS             complement(4148..5701)
                     /locus_tag="OUD09_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HCT9693339.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
BASE COUNT         1799 a         1115 c         1101 g         1765 t
ORIGIN      
        1 aaaaatatca tcgatatcaa acaatcgata atcattctct tcttgtctac ttgaacggat
       61 taatccttgt tcagcccaat agcggatcgt tgattttggc aaatcaaata gttccgatac
      121 ttggccaatc gtatacattt ttttcatttt ttctcacctc tttttcagtt tattcttttt
      181 tctattgacc ttcaagttac ttgaaggtat agaatcgaaa agtatcagga aaaaaggagg
      241 aataatatgt ctatcttaaa aacattctta cagaaaaaca aaggactcgc tctccttacc
      301 tttgtgatga tctgtttaga aatcgccggt acattaggtg taccaaaatt agtagcaaag
      361 ctgatcgaca caggtatagc aagtggcgaa gcctcagtta taaaaaaaat tggagttgaa
      421 atgctgcttg tagcgtttct aggaacaatc gctgcagttg cctctagtta cttttcagca
      481 ttggcttccg ctaggtttgg attccaaacg cgggagaaat atttccgtaa attccaacag
      541 ctgtcaatga aggatatcag tcgttttagt tctggatctc ttttgacaag aatgacgaat
      601 gatgtcgata atgtccagca aatgattgtt ttattttgcc aaatgatctt accagcaccg
      661 gttatttgcg ttttcaccat tctcatgatg tttcgttatt ctctattact aacatgggtt
      721 acattgatct ctgttgtttt ttatgtatgg attgtttatc gactgatgaa acgaggggca
      781 cctttatctt tgagtatcca gccaaaaatg gatcggatca ccatcacatt gagagaattc
      841 tttactggga ttaatatgat ccgtgccttt gataatcaat catatgaaga aaagcgaacg
      901 aataagagct ttgctgacta tgctaatcaa atgatcaaag tgaaccgaat atttgcttgg
      961 gtcactccaa ttgctttttt gctcatggga atcgtttatg cttctatttt gtggtttggc
     1021 ggaaatcttg tagcagaagg agcattacaa atcggtgtcg tgactgctgt agtcgaatac
     1081 tctatgctga cacttgctta cttgatgatc gcagctatgg tattggtcat tatccctaaa
     1141 tcgtttgcct cattacgacg gatagaagaa atactgcaag cagagatcga aattgaggaa
     1201 gagacgatca aaacagtagc tgaaccaacc tttgatcaga caaatgcagc tgcttttgaa
     1261 catgtcacat ttaaatatac tgaaatctct gatccagttc tagaagatat tgatttcaca
     1321 atacctaaag gaaaaacaac agcgatcgtt ggcgggaccg gatctggaaa aagtagtttg
     1381 gcgaaacttc tcttgagact tagtgaccct acgataggaa aagtaaccct tggtggtatt
     1441 cctcttactc aaatgactca agaacagatc cgctcccaca tcagttatgt ccctcagaag
     1501 gctttcttat tcagcgggac aattttaagc aacttgcaga tgggaaatga atatgcctca
     1561 acagaagagt tgtctaaagc gatcaagatc gctcaattag aagaactagt ggacaccctt
     1621 cctgacggat tggacagctt tgttgctcaa ggcggttcta attattcagg gggccagaaa
     1681 cagcggatat gtattgctcg agcgttgatc aaaccagcag agatctatgt attcgatgat
     1741 agtttctccg ctttagacta taaaacagat gcagcattaa gagcagccct tcataaacac
     1801 atgtcggaga aaactctcgt gattgttgct caacgcctca gtacaatcca acaagcagat
     1861 aacatcattg ttttagatga aggaaaaatc gttggacaag gtactcatga agaactactg
     1921 tctaattgcc aaacttatca ggagtttgca aaatcacaag gaatatagaa agaagggata
     1981 cgatggaaaa gaaaaaaacg ttgaacacgc gtgctttgat acgtttttgg aaaatgatca
     2041 aacctgaaca taaatatttt tacggattgt ttctatgcag tttattcggg aacctattag
     2101 tcgtagccat gcccttgatc atgggaatcg ggatcgatga tctcttatca gaaataagaa
     2161 aaacaggtat tggacagctt tctttcagtg atatcaaaca agcattgttg ttccctgtct
     2221 tattgctgat cggctgttca gtcttaagca gcatcacttc atttattcag gaacggacaa
     2281 tggcagcgtt aagcgaaaag atcacattga gggtgcgaaa agaaatcaca aaaaaattca
     2341 aaacattacc gatgtcgttt tttgacaatc atcaagtagg tgacatcatt agtcggacaa
     2401 ctaccggtct caatcaactt tctcaagtgt tattgacagg tatcaatcaa ttttttactt
     2461 ctgtcgtaac aatagctgct gctggtctga tgctgtttta tatcgaaagc aagcttactt
     2521 tgctcgtctt attgctgatc gttggcagtt caattgttac aactaagatt gcgaacaaaa
     2581 acaaacagat ttctgaacag aatcagtatg aactgggtgt tttaaataat cgaatggaag
     2641 aaatcttagc tggtaatcta gtggtcaaaa cattcaatca gcaagctcat gcagaagctg
     2701 ctatacacga agtaaataaa aagcaataca acgcttttaa acgtgctcaa tttatgaact
     2761 gtgctatcta tccggccatt cgtttcatta atcaactcgc cttcatcata agtgcggtat
     2821 tcggtgctat gcttgttcta tcaggtggtg tgacgatcgg cttcttacaa gcttatcttc
     2881 agtacatcaa tcagatttct gaaccaattt ctaccgcttc ttatgtgatc aactcgatcc
     2941 aatccgcaat ggcagcaatc gataggatat ttgagatatt agatgaaaca gatgaaattc
     3001 cagaccttcc agatgcaaga cttttagaac aaccgaaagg aaaaatagct ttcaaaaatg
     3061 tacagtttgg ttacacatca gataaaatat tgatgaatca agtcgatttt tcagtaaagc
     3121 caaaacaaac ggtagcgatc gttggtccaa ctggtgcagg aaaaacgact ttagtcaatc
     3181 tgctgatgcg tttttatgaa ctgaataaag gaaagatcga attcgacggt cttgatatta
     3241 ccaagctttc acgtggagaa ctacgtcgtt cttttgggat ggttttacaa aatacctggc
     3301 tgtttgaagg aacggtagct gataatatcg cctacgggaa aatggatgcc acaagagaac
     3361 aaattatcga agcggctgaa atcgcacaat gtgatcactt tattcgaaca ctgccagacg
     3421 gatatgatac gatcatttct agcgaaaacg gcacgttatc tcaaggacag caacaattat
     3481 tgacaattgc tcgagtcatt ctagcgaatc ctcctgtcgt tattttagat gaagcaactt
     3541 ctagcgtaga tacacgaaca gaagcattga ttcagcaagc aatggaagct gtgacaaaag
     3601 ataggacaag ttttgtgatt gcacaccgtt tgtcaacgat tgaaaatgca gacatgatcc
     3661 ttgtaatgaa gaatggcgat atcgtcgaaa aaggaacaca ttctgaacta ctccaatcac
     3721 caacgcttta tgccagtctt tataatagtc aatttcagat agcgtaacaa acattatcta
     3781 aaaagctatc gatcatgtat gattagtcca tgatcgatag cttttttaaa tgtactagtg
     3841 gtgccactag aaacacaaag aataatacta atgatacttt ttggatagct ccttcagttt
     3901 cgttcggtaa gtagtgacaa aagacttgtc acaatccctt tttacgaata aacggtgttc
     3961 aagagacaac ctatcggcaa taagacacga tcttgtaaaa tacgagaagc tatacttcaa
     4021 aatcgcttct tgttgctctt gtcaactccg aatcacgagt tacactattc aacaaatatg
     4081 agaagctgtt ttttgaattt gtttcttgtg actcggtgct gaacgtcagg ctcacaatct
     4141 ctcttcttta atctccttgt tctacttgcg atagttgctg aacgaattgc catgtttccc
     4201 cgttatcagt tgaacggaag aggcaggcaa acattttccc tgcatcgatt ggtccttttt
     4261 tcaacaaata aaattttgtc tctaacgtat tcgcgcttat ttgagtaact tcatttggtg
     4321 attgaaaaag atctatcccc cctgtctgat agttttccgg aatcgacaga actgattctt
     4381 tgaatgagcg tcctccgttg tttgtataaa acaaactttc acgtgtcgaa acaaaaccta
     4441 aagaagttgt aacgaagctg acattttgta ctggctgatt gatcattgta cttccacttt
     4501 ttatccaagt gattccgcca tccatagtag tgtaaatgta caaagtttct gcagacatcc
     4561 cactggctga tgtacagata aatagaccat cgttgccata aaataccgct tttcgataac
     4621 gtatcggctg aactgattgg gtgataatag acgtttgcca tgttttacca ttatctcttg
     4681 aagtcagcag acgtacatca tccccagaag aatagataaa ccatgagaaa tcggcggaaa
     4741 tatcatatgt gttgtccatc caataacctt caggaatcag ccccgttgtc agggtgtatt
     4801 cgccaaaacg caaccattgc gaatcaatcg gcacaaatac ccaattagca ccactatctg
     4861 ttgtcatata gagagagtgc gtctctgtat caagtttgag atcatttatc ttttgtgttg
     4921 tcaaatttac cttttgctta ggatcagcta acaacccttc atttgtcttt tgtatatgcg
     4981 tatcgttagt tgagttcatt gtcatgtgag aagcagcatt tatatgagaa gacatttttg
     5041 gctgagtgta tctcccataa tagattgttc cagctaaaag aatgaggaaa aaaccagtga
     5101 ctaatgtact catcttagtt gtcaggtatc caccaggcga agactggctt ttttcccatc
     5161 tgggttcgtt ttctagtaaa aaaatatgga atgtgttcat tccgaatttt gatttgcttt
     5221 cgcctaagta gataggtaaa tcatcaagtg atctcagcaa taactggcaa tcactttttt
     5281 gtatccgaaa agcttgactc gtcgaagtct tctcaaaata aagatagtca tcatcttgag
     5341 aagttggttt attatgtaaa aactgttgtg ctctttgact aagccattga tcgaaatgac
     5401 gcgacaattg atggtaacgg agccaaataa gtagaagaaa aacaataagc acaacaattg
     5461 ttattccgcc aactccccac gaaaagtgat acaaattatt taatttttgc atcccttcag
     5521 ctgaacggac tactgcactc atatggcgat ataggaacga tacagctccg aaaaaaagca
     5581 aaccagagaa aaatactaag cttaaaagct tgaacattat tcttttcgct ctttttttcg
     5641 ctttctcgat aattttttgt ttagctgata atacttcgat aataaatagt tcttgatcca
     5701 tttgtctctc cttttctcat acttcattca ttatgcgtca aaatccatac aatcactagt
     5761 cccactaata aaaaatttat
//
LOCUS       DAKBVG010000154         5752 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000154 DAKBVG010000000
VERSION     DAKBVG010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5752
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.154"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            <1..760
                     /locus_tag="OUD09_002100"
     CDS             <1..760
                     /locus_tag="OUD09_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCT9693340.1"
                     /translation="TRSQSGNKYIKTIIEILAHRNYKIYKKNGCVAKNDRVSEKLVQL
                     GVNKVKLAPVCVDFEQLHKNYEAIDFTTLREKWNIPRNVRVILYVGRFEKDKRPLDMI
                     SLFNKLDEHNYLLMVGTGALLEQTKQQINLLGLQNRVKIIERIPNKDIWELYCLSDVF
                     VNLNYTEIWGMVLLEAMYYRLGIIAVNAPGPQNIILDNRYGMLAKSDDEILTFLKDGR
                     FTNDIIDAAYQRVTTEFRWSKSGEIMLSMLEGDK"
     gene            1182..1637
                     /locus_tag="OUD09_002101"
     CDS             1182..1637
                     /locus_tag="OUD09_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HCT9693341.1"
                     /translation="MNAYKLYKVERWFYLHKMEFFAKIVKALIYVLHNSSISYQTEIG
                     DNCKFLYGGIGCVIGKDTKIGNHVIIGTNVLTGGRSNKPGMPTIGNNVYIATGAKILG
                     NITIGDNVIVGANAVVINDVEPNCSVGGVPARVLKKDIDVNDYCSLNRY"
     gene            1651..2424
                     /locus_tag="OUD09_002102"
     CDS             1651..2424
                     /locus_tag="OUD09_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCT9693342.1"
                     /translation="MLYFVIPLMSAKAANNWEMVSTLFNRTLWSCYNQTDPDFKIIVA
                     CHEIPKLTHEYDDRVEFIQVSEKDAPIPTNQDEKMIDKGYKTHALAMRLRELGGGFAM
                     MVDADDLVSSHLAEYVNSHPQENGFYVKTGYVYFVGDDYMKVLPKFSSGSACIVKYAV
                     EDLPEAYPEVMTANCDDNPWIIRKRHGGVVPACEEQGRPLKPLPFKAAVYVLGTGDNH
                     SLYGKTTKYQTKMREILEMFIAKRKIKGKLKAEFSVDWL"
     gene            2411..3541
                     /locus_tag="OUD09_002103"
     CDS             2411..3541
                     /locus_tag="OUD09_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCT9693343.1"
                     /translation="MIGYKVSIMKVLMCESQMPENWYSFDLSKELNQYVDLTLLCKRD
                     VPPLDPKIKSKHILYHKSTNKVFSALVFLKSLFLIWKEIRFGDYDIVHLQLMKSLKYE
                     GGLYVLCKRHYKKLIMTVHNVLPHEVREKDRQEHFNIYSNCDGFIVHNRVTEQHLYAE
                     FPELNAKSYVCPHAIYGDVKHISRAYDKDKFEFILFGQIRPYKGIDVLLNAITELPMD
                     VRKKVHFTIAGQQHPKQDSTDYKKMIEDLKVSDCVLYKPERISDEEMDSMFRNADAAI
                     FPYKEIYGSGALLMAYTYCLPTIVSNVPAFMEETEEGKIGLLFENLNSKSLADTIVKY
                     VNLSPAEKSKIEDDIYELVNRKYTWAASAKLTYEAYKQILNE"
     gene            3572..4807
                     /locus_tag="OUD09_002104"
     CDS             3572..4807
                     /locus_tag="OUD09_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693344.1"
                     /translation="MQISKWKIIQFLMVFLMIISSSILKPLGGWLGETMNVRLVTVIL
                     AIVCLLIASLPAFRKNRYLNFCTLLICVVIIAAYIYSDKTIWVKSRESMSLYYVLLAY
                     PLFCTLINQAWNLDSMLNTICGVTFLSYILRTSISLIQKFSGVLLYENIYRECAPENW
                     YRGGFLRINPPCFTIIIIPIALYMIERQVVARRKVKYYLLIASALAYSAVIHGSRSVL
                     VYQIIVLGCYILFKKGSSKRKIAMWVLAGLLVVIFINSTMFSTFVETFTNSTGEYAGS
                     AESRFASVWFFIPKFLQSPLFGTGILTGEEFTFILPGGVRGHLSDIGFFYTITQYGVG
                     ILIFDILFIIKGFKTALLASKVGMTGYQYLAFGITLNVLLTGINIDWFYPIFTPAIPV
                     CLAVIEYIYQECRSVANIE"
     gene            4832..>5752
                     /locus_tag="OUD09_002105"
     CDS             4832..>5752
                     /locus_tag="OUD09_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693345.1"
                     /translation="MNKKEILYNILKKYENLPYVFRNMFGHNISKLVSWKLTADCATV
                     EENSEVYAAFQLRSKDMSDVPVMGLVNCCKDVAIVMQGPLCLKDDFSYNTLAYYKKCY
                     PNALIILSTWKDEDIKALKRIEDLGVIVICSEKPEKPGHLNINYQVGNTLPGIVRAKQ
                     LGAKYVCKTRTDQRIYHPNAMAFFCSLLEQYPVNNEDDWKLIQNQRLLLLSMPYGDMF
                     FPYCLSDFLYFGDVDDMINLFSIPSDVREKGAVSRGVSRREISENNLAPEVQLLRSYI
                     SRMGGNEECSIRAYWEFVKNHVITINKNQID"
BASE COUNT         1918 a          792 c         1207 g         1835 t
ORIGIN      
        1 aacgcgaagt caaagtggca ataaatatat aaagacaatt attgaaatat tggcgcatcg
       61 aaattataaa atttataaga aaaatgggtg tgttgccaaa aatgatcgag tttctgaaaa
      121 attagtacaa ttaggagtaa ataaagtgaa acttgctcct gtttgtgtgg attttgaaca
      181 gttacataag aattacgaag caatagattt tactactctc agagaaaaat ggaatattcc
      241 tcgcaatgtt cgtgttattt tatatgtagg acggtttgaa aaggataaaa gaccgttaga
      301 tatgattagt ttatttaata aacttgatga gcataattat ttgctgatgg taggaactgg
      361 tgctttactc gagcaaacga agcagcagat aaatttactt ggactgcaga atagagtaaa
      421 aataattgag cgtattccta acaaagatat ttgggagtta tattgcttat cggatgtatt
      481 tgtaaatcta aactatactg aaatttgggg tatggttttg ctagaggcaa tgtactatcg
      541 cctcgggatt attgctgtta atgcacctgg cccacagaat attattttgg ataatagata
      601 tggaatgttg gctaaatctg acgatgaaat tttgacattc ttaaaagacg gacgatttac
      661 caacgatatc atagatgcag catatcagcg tgtaactaca gaatttagat ggtcaaagtc
      721 gggagaaatt atgcttagca tgttggaagg tgataaatga tatggaagta acgaaattaa
      781 tggcattgag gaatcgatat gctttaaaca tagttgataa ttgcacgaga aaaatagcca
      841 aaattcttgg ttgttgtatt ggtaaaggtg cacagatagg gaactctgtt gaatttgttc
      901 ataattctgt aggcactgtg attcattctg acacaatcct tgaagatggt gttaaagtct
      961 atcagaatgt gacgtgtggt tgagcagata gtaatgaacg tatcatcatt cgagtaaaaa
     1021 agaacgctgt tttatgtgtg ggtgctaaaa ttctttgtaa atctaacaca gaattatgcg
     1081 ttggggaagg tgctgtagtt gctgcaaata cagtggtatt gaggtctatt ccaccctacg
     1141 aagtgtgggg ggtacctgct aaaaagatgg ggagataaaa catgaatgca tataaattat
     1201 acaaagtaga acgatggttt tacttacaca aaatggagtt ttttgccaag attgtaaagg
     1261 cactaattta tgtacttcat aatagctcaa tttcatatca aacagaaatt ggagataatt
     1321 gtaagttctt atacggtggc ataggttgtg taattggaaa agatacaaaa ataggtaatc
     1381 acgttataat cggaacgaat gtactgactg gtggtagatc caataagcct ggtatgccta
     1441 caattggaaa caatgtatat atcgctactg gcgcaaaaat tttaggtaat atcacaatag
     1501 gggacaatgt aatagtaggg gctaatgctg tggtcattaa tgatgttgaa cctaattgtt
     1561 cggtaggagg cgttccggct agagtattga aaaaagatat tgatgtaaat gattattgta
     1621 gtttgaatcg ttactaaaag gagagattgt atgttgtatt ttgttattcc tttaatgagt
     1681 gcgaaggcag caaacaattg ggagatggtt agtaccttat ttaaccgaac attatggtcg
     1741 tgttataatc agactgatcc tgatttcaaa atcattgttg cttgccatga aattcctaaa
     1801 ctcacgcatg aatatgatga cagagttgaa tttattcaag ttagcgaaaa ggatgcccct
     1861 attcccacaa atcaagacga aaaaatgatt gataagggtt ataaaacaca tgctctcgca
     1921 atgcgtttgc gagagttggg cggcggtttc gctatgatgg ttgacgcaga tgatcttgta
     1981 agttctcatt tggcggaata tgtgaattcc catccccagg aaaatggatt ttatgtcaaa
     2041 acgggatacg tgtactttgt tggagacgat tatatgaagg ttctaccaaa gttctcatca
     2101 ggaagtgcgt gtatagtaaa atatgcagtg gaagacctgc ctgaggcata tccggaagtt
     2161 atgacagcaa attgtgatga taatccatgg atcattagaa aacgacatgg gggcgttgtt
     2221 ccagcatgtg aagagcaagg gcgtccatta aaacctcttc cattcaaagc agcagtatat
     2281 gtattaggaa caggggacaa tcactcgctt tatggtaaaa cgacaaaata tcagacgaaa
     2341 atgcgagaga tactggaaat gtttatagca aaacgtaaaa ttaaaggaaa actgaaagca
     2401 gaattttctg ttgattggtt ataaggtgag tattatgaag gtattgatgt gtgaaagcca
     2461 aatgccagag aattggtatt cgtttgatct ttctaaagaa cttaatcaat atgttgattt
     2521 aacattgctg tgtaagcgtg atgttcctcc tttagatcct aaaattaaga gcaaacatat
     2581 tttgtatcat aaaagtacaa ataaagtgtt ttcggcactt gtttttctga aaagcttgtt
     2641 cttaatttgg aaggaaataa gatttggtga ttatgatatt gttcatttgc aattgatgaa
     2701 aagtctcaaa tatgaaggcg gattgtacgt tttatgtaag agacactata aaaaattaat
     2761 tatgacagtt cataatgtgt taccacatga ggttcgtgaa aaggatcgcc aagagcactt
     2821 taatatatat tcaaattgtg atggtttcat tgttcacaat cgagtaacag aacagcattt
     2881 atatgctgaa ttcccagaat taaatgcaaa gtcatatgtt tgtccacatg ctatctatgg
     2941 agatgttaaa catatatcta gagcgtatga taaagataag tttgaattta tcttgtttgg
     3001 acaaattcgt ccatataagg gaattgatgt actgcttaat gcaatcacag aacttccaat
     3061 ggatgtaaga aagaaagttc attttaccat agcaggtcaa caacatccta aacaggatag
     3121 tactgactat aaaaaaatga ttgaagattt aaaggtttca gattgtgttc tatataagcc
     3181 agagagaatt tctgatgaag aaatggacag tatgttcaga aatgcagatg ccgcaatatt
     3241 tccatataaa gaaatctatg gaagtggagc gcttcttatg gcatacactt attgcttacc
     3301 aactattgtt agtaatgtac ctgcttttat ggaagaaacc gaggaaggaa agatagggtt
     3361 acttttcgaa aatttgaatt ctaagagcct agcagacaca attgtaaagt atgtgaacct
     3421 tagcccagct gagaaaagca aaatagagga tgatatatat gagcttgtaa accgcaagta
     3481 tacctgggcg gcatctgcaa aactcacata cgaggcatac aaacaaattt tgaatgagta
     3541 gtaaaacaat tagaaaggaa taataattaa catgcagatt agtaaatgga aaatcataca
     3601 gtttctaatg gtctttctaa tgattatctc atcaagcata ttgaagcctt tgggcggctg
     3661 gttgggagaa acgatgaacg ttagacttgt gacagtcatt ctagcaatag tatgtttgtt
     3721 aatagcctca ttaccagcat ttagaaaaaa tagatattta aatttctgca ctttactgat
     3781 ttgtgttgtt ataatagcag cttacatcta ctcagataag acaatatggg ttaaatctag
     3841 ggaaagtatg agtttatact atgtattatt ggcgtatcca ttgttctgta ctttaattaa
     3901 ccaagcgtgg aatttggatt caatgctaaa tacgatatgt ggtgtaacat ttctttcata
     3961 cattcttaga acatctattt cacttattca gaaattttca ggagtacttc tctatgaaaa
     4021 catatatcgt gagtgtgccc cggaaaattg gtatcgtgga ggctttttaa gaattaatcc
     4081 accatgtttt acaattataa taataccgat tgctttgtat atgattgagc gacaagttgt
     4141 agctagaaga aaagtaaaat attatttact gattgctagc gcactggcat attctgctgt
     4201 tattcatggt tctagatctg tccttgtcta tcaaattatt gtacttggtt gctatatcct
     4261 ttttaagaaa ggttcttcga aaagaaaaat tgcaatgtgg gttttagctg ggttgcttgt
     4321 agtcatattt attaattcga ccatgttctc aacatttgtt gaaactttca caaacagtac
     4381 aggtgaatat gcaggatctg ctgaatctcg atttgcttca gtttggtttt ttattcctaa
     4441 atttttgcaa tcgcctttgt tcggtactgg tattcttact ggtgaggaat ttacgtttat
     4501 attaccagga ggagttagag gacacctatc tgatattgga tttttctata caattactca
     4561 atatggtgta ggtatactta tatttgatat tctgtttatt ataaaagggt tcaaaacagc
     4621 attacttgca tcaaaagtgg gtatgactgg ctatcaatat ttagcatttg gaattacatt
     4681 aaatgtcttg ctaacaggga ttaatataga ctggttttat ccgatattta ctccagctat
     4741 tcctgtatgc ttagcggtta tagaatacat ctatcaagaa tgtagaagtg tggctaacat
     4801 agaataggaa atattaatag gagtgcatct catgaataag aaagagattt tatataacat
     4861 attgaagaag tacgaaaatt taccatatgt gtttaggaac atgtttggtc ataatataag
     4921 taaactcgta tcttggaaat tgactgctga ttgtgcaaca gtagaagaaa attctgaagt
     4981 ttatgcagcg tttcaattgc gctcaaagga catgtctgat gttccagtga tgggtttagt
     5041 taattgttgc aaagatgtag caattgtaat gcaggggcca ctatgtttaa aggatgattt
     5101 ctcttataat actctggcct actataagaa atgttatcca aatgctttaa ttattctttc
     5161 gacatggaag gacgaagata ttaaggcatt gaaaaggata gaggatcttg gagttatagt
     5221 tatttgtagt gagaaacctg aaaagcctgg acacttaaat attaattatc aagttggaaa
     5281 cactttgcct ggaatagtca gagcaaagca gcttggtgca aagtatgttt gtaaaactag
     5341 aacagatcaa agaatatacc atccaaatgc aatggcgttt ttctgcagtt tacttgaaca
     5401 gtatcctgtt aataacgaag atgattggaa attaatacaa aatcagcgat tactgttact
     5461 atcaatgccg tatggagaca tgtttttccc gtattgtttg tcagacttcc tgtattttgg
     5521 tgatgttgat gatatgataa acctcttctc gattcctagt gatgtgagag aaaagggagc
     5581 tgtgtctaga ggagtctcaa gaagggagat atctgaaaat aatttagctc cagaagttca
     5641 gcttcttcga agctatattt cacgaatggg gggcaatgag gagtgctcta ttagggcata
     5701 ttgggagttt gtaaaaaacc atgttattac tattaacaaa aatcaaattg at
//
LOCUS       DAKBVG010000155         5649 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000155 DAKBVG010000000
VERSION     DAKBVG010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.155"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(322..1083)
                     /locus_tag="OUD09_002106"
     CDS             complement(322..1083)
                     /locus_tag="OUD09_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HCT9693346.1"
                     /translation="MEKNMYKRKRDFTVCGIVGVCATTKNEFVREVFSMPTSPVSATV
                     DLVGVPAIISAKENPKIAEMYSQATMAAIDGMPIVKIGRRKGFTCERCAAPDIMGMVF
                     EEGVRQGKTHYFYGGKDNEVLNKLRANLERDYPGIKIVGMYSPPFRPLNEEEDSKICE
                     EINNLNPDFLWVGIGAPKQEMWMWEHQKKIHNTVMFGVGAGFNFFAGTLEKAPEWMEK
                     ASLEWLFRLVKEPRRLWRRYILGGIKYIYYTIESK"
     gene            complement(1159..2157)
                     /locus_tag="OUD09_002107"
     CDS             complement(1159..2157)
                     /locus_tag="OUD09_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCT9693347.1"
                     /translation="MKKTIMVTGGCGMIGSNLVKRLVKEGCWDVYVADNLWRGKLEYL
                     NDEDGHPVIDLDTHFFNADLTDYEQAKKVIGTTEYVVHLADVVAGIDYVFKNQGELFR
                     INNLINSNVFDCCRKAGKEKIKGVIYVGTVCSFPLTRQNTLNPEPLREEELFPALPES
                     AYGWSKLMGQLEMGYLEKETGIPCCILMLHNVYGTPTDFGERSQVIPALIRKAINNPE
                     EPFNVWGSGEQGRAFIHVNDIVNALVLALDKGWGHGHIQIGPSHCTSIKEIAYKIIEI
                     SGKDIKPFFDTSKPEGDKARCADYSKAKEILDWEPTVSLEEGLRESYNWIEKQIKG"
     gene            complement(2176..2829)
                     /locus_tag="OUD09_002108"
     CDS             complement(2176..2829)
                     /locus_tag="OUD09_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCT9693348.1"
                     /translation="MLAEKLNKHIDLLIKRYPTLETIKQDIIDGYLIMEECYLNGGKL
                     LVAGNGGSAADSEHIAGELMKRFKTPRPVSKEFADKLVAIDPVRGPELAKNLECGLTA
                     IPLVAHEALSTAYINDVDGLGVFAQQLFGFGRSGDVFLGISTSGNSKNIMSATVVARA
                     LGIKVIGLTGAKGGELATVADVCVKAPEVETYMIQEYHLPIYHCWCLMLEDKFFGNE"
     gene            complement(2839..3849)
                     /locus_tag="OUD09_002109"
     CDS             complement(2839..3849)
                     /locus_tag="OUD09_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HCT9693349.1"
                     /translation="MIITKTPFRMSFFGGGTDMEDYFKENGGAVLSTTFDKYCYVNVR
                     HLPRFFDYSAELSYSRTERVTSIEDIEHPAIRNAMKMLDMHEIRLTYEADLPARSGLG
                     TSSSFAVGMLNAFYALEGKYADKKKLADEAIYLERVLCNEAGGWQDQIAASYGGFNRI
                     NFNVDGYEVLPVIISPERKKQLNNNLMMFFTGFTRFSSDVQKANNVSGTEDKRVRLKK
                     MYELVDEAEAILTDKNRNLDDFGRQLDVTWRLKKGTGGAISTGSIDELYEKGMAAGAL
                     GGKLLGAGGGGFLVFYVQPEKQDAVRWAMRDLMYIPFEFENGGTRVIHYTPETYIEKQ
                     NG"
     gene            complement(3868..5118)
                     /locus_tag="OUD09_002110"
     CDS             complement(3868..5118)
                     /locus_tag="OUD09_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HCT9693350.1"
                     /translation="MKKQYNIAVAGTGYVGLSIATLLAQHHKVTAVDIIPEKVELINN
                     RKSPIQDDYIEKYLAEKELNLTATLDAKAAYQSADFVVIAAPTNYNSRTQHFDTSAVE
                     AVIRLVMEVNPDAIMVIKSTIPVGYTASVRVKTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMDDERLVEDAHTFATLLQEGAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPENLIEAIVESNRTRKDFIADRVLQMAGYYNYAEANQYDAGKEKPVVIGVYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPTLKDGETFFGSKVVNDLEQFKAMCDSVI
                     ANRYDACLDDIKEKVYTRDIFQRD"
BASE COUNT         1659 a         1399 c          917 g         1674 t
ORIGIN      
        1 atgagtatac atgaggtact gatagttgaa ggtccgcaaa ttgtattaga acatccgcat
       61 tcttatcaaa attctttgtt gataagaatc catttgatcc tacataacga cacttgatat
      121 agtgaatcat cgccgtattg cttaattgct ctgttccaac ttcctcatca ctttttacac
      181 ttttatatac aataacttcg tggcctaatt cagctaaccc tcgagctaat cctatttcct
      241 gagaattata ggaccccttc ttaccaaagt tattcgtcat tgtaactact ataagtattt
      301 tcatctattt ttattgcccc cctacttaga ttcaatagta tagtagatat actttatacc
      361 tcccaagata taccgcctcc atagtctacg aggttccttt accagtctaa ataaccactc
      421 tagactagct ttttccatcc attcaggagc tttttccaaa gtgcccgcaa aaaagttaaa
      481 gccggcgcct acaccgaaca ttactgtgtt atggatcttc ttctggtgtt cccacatcca
      541 catttcttgc ttaggcgcac caataccaac ccacagaaaa tctggattta agttgttgat
      601 ttcctcgcaa atcttgctat cctcttcttc atttaacgga cgaaatggtg gggaatacat
      661 tccaacaatt ttgattcccg ggtaatctct ttctaggttt gcacgaagct tatttaacac
      721 ttcgttatct tttccaccat agaaataatg tgtttttccc tgtctaacgc cttcttcaaa
      781 aaccattccc ataatatctg gtgcagcaca tcgttcacag gtaaatcctt tgcgccgtcc
      841 aattttaaca ataggcatac catctattgc agccattgta gcctggctat acatttcagc
      901 aattttggga ttttcctttg ccgaaataat ggccggcact cccacgagat caacagtcgc
      961 acttaccgga gatgttggca tggaaaaaac ttcccttaca aactcgttct ttgtagtagc
     1021 acaaacacct actattccac acacagtgaa atcacgtttt cttttataca tattcttttc
     1081 catctcagta catccttact catatgttct tcctccgcat cctctaatta cacggaggaa
     1141 gaacagctta tatacttatt aacccttaat ctgcttttca atccaattgt aggattcacg
     1201 aagaccctct tccaaagaaa cagtaggctc ccaatccaaa atctctttcg ccttagaata
     1261 atctgcgcaa cgagccttat caccttcagg cttagaagta tcaaagaaag gcttgatgtc
     1321 tttgccagaa atttcaatga tcttatatgc aatttccttg atggaagtac agtgagaagg
     1381 gccaatctga atatgaccat gaccccaacc cttatccagg gcgagcacca atgcatttac
     1441 gatatcattg acatgaatga aagcacgtcc ctgctctccg ctaccccaca cattaaaagg
     1501 ctcttcagga ttattgattg ctttacgaat cagagcagga ataacctgac tacgttcgcc
     1561 aaaatcagta ggtgtgccat aaacattatg gagcatcagg atacagcacg gaattccggt
     1621 ttctttttcc aaatacccca tctccaactg gcccatcagt ttgctccagc cataagcaga
     1681 ctcaggaaga gcagggaaca actcctcttc acggagaggc tcaggattca aagtattctg
     1741 tctggtcaga gggaaggagc atacggtacc aacatagata acacccttga ttttctcctt
     1801 gccagcctta cggcaacaat caaaaacatt agaattgatc agattattga ttcggaacaa
     1861 ttcgccctgg ttcttaaaca catagtcaat gccagccaca acatcagcca gatgaactac
     1921 atactcagtc gtaccaataa cctttttagc ctgctcataa tcagtcagat cagcattgaa
     1981 gaaatgagta tccaggtcga tgacaggatg cccatcttcg tcgttcaggt actccaactt
     2041 tcctctccac agattatcag ctacataaac atcccagcag ccttccttca ccagtctctt
     2101 aacaagattg gagccaatca taccgcaacc accggtaacc ataattgtct ttttcattta
     2161 acattcctcc aaattttatt cgtttccaaa gaatttatct tccagcatca aacaccaaca
     2221 atgatatatc ggcaagtgat actcttgaat catgtatgtt tcaacttcag gagccttaac
     2281 acaaacatct gctaccgtgg caagttcccc accctttgcg cccgtcaagc caataacttt
     2341 tataccgaga gccctagcaa caacagttgc gctcataata ttcttgctat ttccggacgt
     2401 cgaaatacca aggaatacgt caccagatct accaaagcca aacagctgct gtgcgaagac
     2461 acccaaacca tcgacatcat taatataagc agtagacaat gcttcgtgtg cgaccaaagg
     2521 aattgccgtt aaaccacact cgagattttt ggctaattcc ggacctctta cagggtcaat
     2581 tgcaactaac ttatcggcaa attccttact tacaggacgt ggcgtcttaa accgcttcat
     2641 caactcgcca gcaatatgtt ccgagtcagc ggctgatcct ccgtttcctg ccacaagaag
     2701 tttaccacca ttcaaatagc attcctccat aattaggtat ccatcgataa tgtcctgttt
     2761 aatcgtctca agtgtaggat atcgtttaat caataagtca atatgtttat taagtttttc
     2821 cgctaacatt gttactcctt atccattctg tttttcaata taagtttccg gcgtgtagtg
     2881 aataactcta gttccaccat tttcaaattc aaacggaata tacatcaaat cacgcatagc
     2941 ccaacgaact gcatcctgtt tctcaggttg tacatagaaa accaagaaac ctccaccacc
     3001 tgcaccgagc aactttccgc ctaaagcacc tgcagccata cccttttcat atagttcatc
     3061 aatgcttcct gtactaattg ctccaccagt accttttttc aatctccaag taacatcaag
     3121 ctgtctaccg aaatcatcta aatttctgtt cttatcagta agaattgcct cagcttcatc
     3181 tacaagctca tacatctttt tcagtcttac tctcttatcc tctgtgccac tgacgttgtt
     3241 agccttttga acatcagaag aaaatctagt gaatccggta aagaacatca tcagattgtt
     3301 gttaagctgc tttttacgtt caggcgaaat aatgacaggc agcacctcat aaccatccac
     3361 attgaaatta attcgattga acccaccata agatgcggca atctgatcct gccatccacc
     3421 ggcttcatta cacagaactc tttcaagata aatggcttca tcagcaagtt tcttcttgtc
     3481 agcatactta ccctccagtg cataaaaagc attcaacata ccaactgcaa aagaactgga
     3541 tgtacccaga ccagagcgag caggtaaatc agcttcgtat gtaagccgaa tttcatgcat
     3601 atcgagcatt ttcatcgcat tacgaatcgc aggatgctca atatcttcaa tgctagttac
     3661 tctctcagta cgagaataag ataactcagc gctgtaatca aaaaatctag gcagatgtct
     3721 cacattcaca taacagtact tatcaaaagt agtagaaaga actgcaccgc cattctcttt
     3781 gaaataatcc tccatatccg ttccaccgcc aaaaaaagac atacgaaacg gtgtttttgt
     3841 aataatcata aatactctct cctaatctta gtctctctgg aagatatcac gggtatatac
     3901 tttttcctta atgtcatcaa ggcaagcatc gtagcgattt gctataacag aatcacacat
     3961 tgccttaaac tgttccaaat cattcacaac cttactgcca aagaaagtct ctccatcttt
     4021 caaggtcggc tcataaatca caacagttgc acctttagct tttacacgct tcataacgcc
     4081 ctggatagaa ctctggcgga agttatcaga attggatttc atggtcaaac ggtaaacacc
     4141 aatcacaaca ggcttttctt tgccagcatc atactgattt gcttctgcgt aattgtaata
     4201 accagccatc tgaagtacac gatccgcaat aaagtcctta cgagtacggt tagattcaac
     4261 gattgcctcg atcagatttt caggaacatc tgcgtagttg gccagaagct gcttggtatc
     4321 cttcggcagg caatagccac cgtaaccaaa ggaagggttg ttgtaatgag taccaatacg
     4381 aggatcaagg caaactccat taatgatctg ctgagtgttc aagcctttca tctctgcata
     4441 agtgtccagc tcattgaagt agctgacacg aagagccaga tatgtattgg caaacagctt
     4501 aacagcttct gcttcagtaa agcccatgaa caatgtgtcc acattctctt tgattgcgcc
     4561 ttcctgtagc agtgtagcaa aagtgtgtgc atcctcaacc aaacgctcat catccatatc
     4621 ggtacccaca ataatacgcg aaggatacag attgtcatac agtgccttac tttcacgcag
     4681 gaactcaggg ctgaaaataa tattcttgct accagtcttt acacgaacag aagcggtgta
     4741 tccaacagga atggtggact taataaccat aatggcatca ggatttactt ccatgaccag
     4801 cctaataaca gcctcaacag cggatgtgtc aaaatgttga gtgcggctat tgtagttagt
     4861 aggagccgca ataacaacaa aatccgcaga ctggtatgct gcctttgcat ccaatgttgc
     4921 ggtcagattc agttctttct cagccaagta cttttcaata taatcatcct ggattggaga
     4981 tttacggtta ttaatcagtt caaccttctc tggaatgata tccaccgctg taaccttatg
     5041 gtgctgtgcc aacagagtgg caatactcaa accaacataa ccggtgccgg cgactgcaat
     5101 gttatactgt ttcttcataa tatttcctct ttccaccact gctgtggtca ctactttctt
     5161 aacaaaatgc tgtttgtgaa cagtaacctg ctatttatat aaaacagccc gaaagctgtg
     5221 ttcattcata cttaaaacag ccagctccta cgccggcaaa ttcaattttt cataaatccc
     5281 ctacccgttt agggttatat tgcaagtttg agtcattttt attgacctcg tttggggtta
     5341 taatacattt tcgcattgaa atgtacctac attttccact caaccagtca ccaaaaagtt
     5401 tataaattca cttattaccc tctattctta ctccaaaggt atgtttaaca tcgctttatt
     5461 gacctatttt caccccaaat ggaagtttgt ttggtacatt attggtctat tcccacccca
     5521 aactgtctat tttgagcgag tttttgtttt ttatcattgc acattttcaa ggctcaaatt
     5581 ctaagcggat ttactggcag ggagacattg gtaaatactt gaacaatttc acggctttca
     5641 tcatcacat
//
LOCUS       DAKBVG010000156         5602 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000156 DAKBVG010000000
VERSION     DAKBVG010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.156"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     regulatory      123..220
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            336..917
                     /locus_tag="OUD09_002111"
     CDS             336..917
                     /locus_tag="OUD09_002111"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCT9693351.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            920..2227
                     /locus_tag="OUD09_002112"
     CDS             920..2227
                     /locus_tag="OUD09_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HCT9693352.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            2643..3131
                     /gene="purE"
                     /locus_tag="OUD09_002113"
     CDS             2643..3131
                     /gene="purE"
                     /locus_tag="OUD09_002113"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCT9693353.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            3124..4248
                     /gene="purK"
                     /locus_tag="OUD09_002114"
     CDS             3124..4248
                     /gene="purK"
                     /locus_tag="OUD09_002114"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCT9693354.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            4261..5556
                     /gene="purB"
                     /locus_tag="OUD09_002115"
     CDS             4261..5556
                     /gene="purB"
                     /locus_tag="OUD09_002115"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCT9693355.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
BASE COUNT         1742 a          958 c         1302 g         1600 t
ORIGIN      
        1 acgttgtcag tttcagaaag tagtgttaga ctgttttcaa tatttgaaac aacatttttt
       61 attcaaatat ggcaaactgc cttcacgtgc aaaacagttg caagaaaatg aatttacact
      121 attattagtt ttacatacct acttatatat cgtcataata tggatgacag tttctagcca
      181 gtaccgtaaa tgctggacta taagtaaaag attggctatt tcacccttct tttcttatga
      241 taaagtggag ggtgtttttc tttaacagaa acatagtagg tttttgcaca aaagcggtta
      301 tttcggtatt aataaatgat aaaggagcaa ataaagtgaa agaattagtt gaacgtattc
      361 aaaaagacgg acgtatatta ggcgagggcg tattaaaagt cgacagcttc gtcactcacc
      421 aagtagaccc tgtattgatg gaacaaatgg gaaaacggtt tgcagaagta tttaaagaac
      481 aaggaatcac gaaagttgtg accattgaag cttctgggat cgcaccagct ttatttgctg
      541 caagagaatt agatgttcca atgatcttcg ctagaaaagc aaaaagtttg acgatggatg
      601 aagaattatt gacagcttct gtttattctt ttacgaaaca agtgacaagt acgatttcta
      661 tttctcgtaa atttttatct tcagaagaca aagtattgat tatcgacgat ttcttggcaa
      721 acggacaagc ggcaaaagga ttgatcgaat tatgccagca agctggagcg caagtagaag
      781 gaatcgggat cgtcatcgaa aagtctttcc aagatggacg tcagttactt gaagaaatgg
      841 gattgagagt cgtttctctt gcgcgtattg cttccttgac gaatggacaa gttgagtttc
      901 ttgaggagga cgcataatcg tggaaaaaga aacacaaaat ggaaaagctg cggttttagg
      961 gctgcagcat ctgctagcga tgtatgcagg agcagtagcg gtgcctcttt tgatcggtac
     1021 aggactaggc tttaatcaag aacagatgac ttatttgatt tcaattgata tctttatgtg
     1081 tggattagcg acattattgc aattgacagt taaccgattt ttcggaattg gcttgcctgt
     1141 cgttttagga tgtgcgatcc aagcagtagc tccactgatc ttgatcggaa ccaatgaagg
     1201 agtaggagct atctacggat ctattatcgc ttctgggatc tttgttgtgt tagtttctgg
     1261 tgtcttttct aaaattaaaa aactattccc tcctatagta acaggaacgg ttatcaccgt
     1321 tattggtttg actttgattc ctgtagcaat cgagaaaatg ggtggaggta atgcaacagc
     1381 agctgatttt ggtgacaaaa caaatctgct acttgcattt gtgacgattc tcctaattgt
     1441 aggcgtgcaa atgctggcaa aagggttcat tcgttcaatc gcggtattga ttggtttagt
     1501 cggaggaagc atcttagctg ctttcatggg aatgattgat ctgagcgcga ttggagatgc
     1561 gccgttgttc catgttccac aaccctttta ttttggtaaa ccaactttcg acgtatggtc
     1621 gattttacta atgatcatca tttccatggt cagcatggta gaatcaacag gtgtatattt
     1681 tgcacttggt gacattactg ggaaaaatgt tggagaagaa gaattgaaaa agggatatcg
     1741 tgcagaagga cttgccgtta tcttaggcgg tattttcaat acattccctt atactggctt
     1801 ttctcaaaat gtcggattgg ttcaactttc gggaatcaaa acaagaaaac ccatctattt
     1861 ctctgcattc ttcttgattg ttttaggatt attccctaaa atcggtgcgt tagcacaaat
     1921 catccctgag ccggtacttg gtggaggaat gttagtcatg ttcgggatgg tggcagtcca
     1981 aggaatccgt atgctgttgg aagttgattt cagcaatgat aaaaatctat tgattgttgc
     2041 tgtttctgtt ggatttgggc tgggtttcaa tgttacgccg acattattca gtcaaatgcc
     2101 gcaaacattg caaatgttca ctggaaatgg catcgtaatg agtagcatta cggctatcat
     2161 attgaatttg atctttaatg gattgaaaaa agatgaagaa atattggaag aagccttcaa
     2221 gaagtaatag agcaaaagaa aggttgtgaa atcagcgttt tgacctgatt gttagagaaa
     2281 caaacggaaa aatagttcct cgtattttgc cgtttgtttg agctaatacc gagggtcagc
     2341 tgattgaaca ccgttgaagg aagaggtcgt gagcctgccg ttcatctccg agtcacaagg
     2401 aacaatttca aaaaaaagct tctcatgttt gttgaatatt gttccttgtg accgaggagt
     2461 tggcaggaga acaccgttta tccataaaaa ggaatgtgac ctaacttttg tcacattcct
     2521 tttttttatc tttaataaac acgaacaata aaatgttttt ctttgttaac attcgaaatt
     2581 tctttgacat caaaaaaagt gattgttaaa atatgaatgt agaaaaactg ggaggaaaaa
     2641 caatgacagc aaaaatctct gtgattatgg ggagtacttc agattgggaa acaatgaaag
     2701 aagcatgtca aatattggat gaattcggtg tgtcttatga aaaaaaggtc gtttctgctc
     2761 atcgaacccc agatttgatg tttgaatatg cacaaaacgc tcgaaaaaaa ggaatcaaaa
     2821 tcattattgc aggagcaggg ggagccgcgc atctgccggg tatggtcgca tcaaaaacaa
     2881 ctttgccggt tatcggtgta ccggtccaat cacgaacatt aaatggtttg gattcgcttc
     2941 tttcgattgt ccaaatgcct ggaggagtac ctgtagctac tacagcaatc ggaaaagcgg
     3001 gagcaattaa tgctggacta cttgcgatac agatgctttc catgtatgat ttagaattgg
     3061 aggctaaatt ggctgaaaga cgttcaatgt tggctaaaac tgttatagaa agtagtgatc
     3121 aacttggtta aacctttgtt gcctggttca acgatcggga tcgtcggtgg cggtcaatta
     3181 ggtcgtatga tggcgattag tgcaaaagaa atgggcttta aagtaggtgt gctggatcct
     3241 gtgagcgatt gtccagccgc acaagttgca gattggcaca ttgaaagtgt ctatgatgac
     3301 acctttgcgc tgaaagagct tgccagaaga acagatgtca ttacctatga attcgaaaac
     3361 gtcagtgtag aggcgttaaa tgcgatttta cccatgtcat ttattcctca agggacagac
     3421 ttgcttgcga ttacgcaaga ccgcctgtta gaaaaatctt ttttggaaac gaataacatc
     3481 gtgattgctc cttatgcaac gatcgtcagt ccgacggata tccaagatgc gattgacggg
     3541 atcggctatc cttgtgtttt gaaaaccact cgaggcggct atgatggcaa ggggcagtat
     3601 gttttgaaaa gtcgggcgga tctagctcct gccatggacc ttttaagaga aggaacctgt
     3661 gttttagaag catggatccc gtttgaaaaa gagatttcga tcatggtggc aggaaatggt
     3721 caaggagatt tcacgacttt tccggtcgtg gaaaacatcc accacaacaa tattcttcat
     3781 gaaacgattg ctccagccgc gattgatcag gatgtgatcg aggaggctga gcgtattgcc
     3841 cgtgtgattg cagaagcagt gagcctttca ggtgttttag gggtagaaat gtttttaaca
     3901 aaaacaggcg ggctatatgt gaatgaatta gctcctcgtc ctcacaattc tggacattat
     3961 tctattgaag cttgttctat gagtcagttc gatgcccata ttcgaggtat ttgtggttgg
     4021 ccattaggag aaaaagtacg tttgataagc aatgcagtaa tggtcaatgt tttgggagaa
     4081 caactgctgg aaagctatcg cttgattgag aataagccag actggcaatt ccatttctat
     4141 ggaaaaaaac aagcaaaaaa aggtcgcaag atgggacata tcactatctt gacagaagac
     4201 atccagcaaa cgctggaaga aatcaaacaa acgaatattt gggattagag gagaaaaaac
     4261 atgttagaac gttatacacg ccctgaaatg gggaatatct ggacggaaga gaacaaatac
     4321 cgtgcgtggc tcgaagtcga gattttagca gatgaagctt gggcagaact tggtgaaatc
     4381 ccgcaagaag atgtgaaaaa aattcgtgag catgcatcat ttgatatcga tcggatcttg
     4441 gaaatcgaaa aattaacacg ccatgatgtc gttgcgttta cacgagcggt ttctgaaaca
     4501 ttgggagaag aaagaaaatg ggtccattat ggtctaacaa gtacggatgt cgtagatact
     4561 gcttatggct atctcatcaa gcaagcaaat gatattttac gggaagactt gaaacgtttt
     4621 acggaaatca ttggcgaaaa agcaaaagaa cacaaatata cagtgatgat gggtcggaca
     4681 cacggtgtcc atgctgaacc aactactttt ggattgaaac ttgccacatg gtattcagaa
     4741 atgaaacgga acatcgaacg atttgaacat gcggcaaaag gagtagaagc aggaaaaatc
     4801 tcaggagcag tgggaacgtt cgcaaatatc ccgccattta ttgaagagta tgtatgtaaa
     4861 caattgggga tccgtccaca agaaatctct actcaagttc tccctcggga tctacatgct
     4921 gaatatttct cagcgatggc gcttattgct acaagtattg aacgttttgc aacagagata
     4981 cgcggattgc aaaaatccga aacacgtgaa gtagaagaat tttttgcgaa aggtcaaaaa
     5041 ggttcttctg ctatgccgca caaaaggaat ccgatcggtt cagaaaatat ggctggtcta
     5101 gccagagtgg tgcgcggaca catggtcact gcctatgaaa atgtcacatt atggcatgaa
     5161 agagatattt ctcattcttc agcagaacga atcatcttgc ctgataccac gattttattg
     5221 aattacatgt taaatcgttt tggcaatatc gttaaaaatc taacggtctt tcctgaaaac
     5281 atgaagcgga atatgtctgc aacatttggt ttgatttata gccagcgcgt tatgctgaaa
     5341 ttgatcgata agggaatgac aagagaagaa gcttatgatc ttgttcagcc aaaaactgcg
     5401 tacgcttggg acaaccaagt gcagttccgc ccgctgttag aagaagatcc aaccatcaca
     5461 tcggtattat ctaaagaaga aatagacgat gcctttgatt atcatcatca tttgaaaaac
     5521 gttgacgtga tattcaaacg agtaggtttg gaataaagaa gagtgagttg caaaattcaa
     5581 ttaaaataga atagcaaaaa ta
//
LOCUS       DAKBVG010000157         5578 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000157 DAKBVG010000000
VERSION     DAKBVG010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.157"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            181..2175
                     /gene="uvrB"
                     /locus_tag="OUD09_002116"
     CDS             181..2175
                     /gene="uvrB"
                     /locus_tag="OUD09_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HCT9693356.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            2185..5001
                     /gene="uvrA"
                     /locus_tag="OUD09_002117"
     CDS             2185..5001
                     /gene="uvrA"
                     /locus_tag="OUD09_002117"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCT9693357.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            5264..>5578
                     /locus_tag="OUD09_002118"
     CDS             5264..>5578
                     /locus_tag="OUD09_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693358.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQE"
BASE COUNT         1839 a          931 c         1286 g         1522 t
ORIGIN      
        1 aatgaaaaaa atgcgagttt ctaacatttt atgttataaa acgtaacatg tataataaga
       61 taaaaaagtg gcgaaagtat gttcggtgtg gtataatttt ttagtaagga atgcctttgc
      121 tttccttttc tttttattgg tcgtaaaatg aaaaggaatg aatgaaaagg aggacgaaga
      181 atgattgaga gagatacctc ccgacacttt gagcttgtct cgaaatacca accggctggt
      241 gatcagcctg aagcgattaa ccaattagtg gatggcgtag tcggcgggaa aaaagcacaa
      301 atcttattag gtgcgactgg aacagggaaa acatatacga tttccaatct tattgagaaa
      361 gtgaataaac caacactgat tattgctcat aataaaacat tggctggtca gctgtacggg
      421 gaattcaagg aatttttccc taacaatgct gttgaatatt ttgtttctta ttatgattat
      481 tatcagcctg aagcttatgt gccttcaagc gatacgtata tcgaaaaaga ttcaagtgta
      541 aatgatgaga tcgacaaatt acgacattcg gctactagtt cattattaga aagaaatgat
      601 gtgatcgtca ttgcatccgt ttcatgtatt tttggattag gttcgccttt tgagtaccaa
      661 aaacaagtag tttcgattcg acagggagca gaactagatc gcaatcagtt gattcgtgac
      721 ttagtcagta tccaattcga aagaaatgat atagattttc agcgcggtcg ttttcgagtg
      781 cgcggtgatg tcgtggagat ttttccggct tcaagagatg aacgtgcctt acgtgtggag
      841 ttttttggag atgaggtcga acgtatccga gaagtcaatg cactgacagg tgaagtttta
      901 ggagaaacgg aacacgtggc aatcttcccg gcgactcact ttgtgacgaa tgatgaacat
      961 atggagcatg ccgtagcgaa tatcaaagcg gaattagaac agcgtttaac tgtcttgcga
     1021 aatgaaaaca aacttctgga agctcaacgt ttagaacagc gaaccaatta cgatattgaa
     1081 atgatgttag aaatgggcta cacgtcggga attgaaaatt attctcggca tatggacgga
     1141 cgtaaagaag gtgaaccgcc ttatacgcta ttggatttct ttcctgaaga ttttttgatc
     1201 gtggcggacg aatcgcacgt aaccatgcct caaatcagag gaatgtataa tggtgaccga
     1261 gcgagaaaac agatgctagt agattacggt ttccgtttgc catctgcttt agacaatcgg
     1321 ccacttcggt tagaagagtt tgaaaaacat gtcaatcaga tcatttatgt ctctgcgacg
     1381 ccaggaccat atgaacatga acaaacagat accgttatcc aacaaatcat ccgtccgaca
     1441 gggctgcttg acccagtgat cgaagttcgt ccaatcatgg gacaaatcga tgatctagta
     1501 ggcgaaataa atgaacgagt cgaaaaagac cagcgggtgt ttgtaacgac attgacgaaa
     1561 aaaatggcag aagacctcac agattatttc aaagaattag ggatcaaagt caaatatttg
     1621 catagtgata tcaaaacgct ggaacgaaca gaaatcattc gagatcttcg attaggtgaa
     1681 tttgatgtgt tagttggaat caatttgctt cgtgaaggat tagacgtacc tgaagtttcc
     1741 cttgttgcta tcttggatgc ggataaagaa ggctttttac gtagtgaacg ttccttagtc
     1801 caaacgattg gacgagctgc tcggaatgaa gaaggtaaag taatcatgta cgcagacaaa
     1861 gtaacagatt ctatgcgtct agcgatggat gaaacgtcaa gacgtcgtac gattcagcag
     1921 aagtacaatg aagaacatgg gatcgttcca aaaacgatca ttaaagaaat ccgagatttg
     1981 atttctatta cgaaagaatc ggaagatgat acaaaagaag cggtacaagt ctcgtacgaa
     2041 gaaatgacga aggaagaaaa agacacgtta ttgatgaaat tagaaaaaga aatgaaagat
     2101 gcggcaaaag ctttggattt tgaaacagcc gcgaatgtgc gagatatgat tttagaatta
     2161 aaagcttcaa agtaggagga caacatggct aacgataaaa ttacgattca tggtgcacgt
     2221 gcccataact tgaaaaatat agatgtaacg attccacgag ataaattcgt ggtcgttaca
     2281 ggtttgtccg gttctggaaa aagttcccta gcatttgata cgctgtacgc tgaagggcag
     2341 cggagatacg tagagagcct ttctgcttat gctcgtcagt ttttaggaca aatggataag
     2401 ccggacgtag atagcatcga cggattgagt ccggcgattt ctattgatca gaaaacaaca
     2461 agtaaaaatc ctcggtcgac agtaggtact gtgacggaaa tcaatgacta tttacgcttg
     2521 ttgtttgcac gtgtaggaca tccgatttgt cccaacgatc atatcgagat taccagtcaa
     2581 tcagtagatc aaatggcaga tcaagtaatg gctttgcctg agcgcacaaa gatccaagtg
     2641 ttggcaccga tcgtggtaaa gaaaaaagga caacataaaa aaatattcga acgaatccaa
     2701 aaagagggat acgtccgcgt tcgagtcgat ggtgagatct atgatttaag tgaagtgcct
     2761 gagctggaga aaaacaaaaa acatgatatc gcgatcgtca tcgaccggat cattgtgaaa
     2821 gaagggattc gttctcgcct atttgattct ttagaggcgg ccctgcgatt agcagacggc
     2881 tatgctctgg ttgacgtgat cggtgaagaa gaaatgttgt ttagcgaaca ctatgcctgt
     2941 ccttattgcg gatttactgt tggcgaacta gaaccaagac tgttctcatt caatgcgcct
     3001 tttggagctt gtccagactg tgatggttta ggtgtcaaat tagaagtcga tcaagattta
     3061 gtgattccag atagaacgaa gacacttagt caaggtgcta tcgttccatg gaatccaatc
     3121 agctctcagt attatcctca aatgttagcg caggcttgtg aaagtttcgg tatcgattta
     3181 gatacaccat tcgaagaatt accggaagag catcaggaga tcatcttgaa tgggacagat
     3241 gatcttttcc atttccatta tgaaaatgat tttggtggcg ttcgcgatgt agaaacgact
     3301 ttcgaagggg tattgaagaa tatcgagcgt cgttatcatg atacgaacag tgatttcacg
     3361 cgtgagcaaa tgcgtcttta tatgacagaa ttaacttgtc agacatgtaa aggttatcga
     3421 ttgaatcccc aagctctatc tgttcaaatc aatggaacga atatcgggca aacaaatgag
     3481 ttgtctattc aaaaagcagt agatttcttc tcaaacttaa cgttatctga acaagagaaa
     3541 gtaattgcca aaccgatttt aaaagaagta aatgacagac tttcattctt ggaaaatgtg
     3601 ggactagact atctaacttt gagtcgcgct tccggaacat tatctggggg agaagcgcag
     3661 cgaatcagat tggctacaca aattggatcg aatctttctg gagttttgta tattttagat
     3721 gaaccatcga tcggattgca tcaacgcgac aacgatcgac tgattggctc attaaagaaa
     3781 atgcgtgatc ttggcaacac cctcgttgta gtcgaacatg atgaagatac gatgtgggca
     3841 gcagattatt tgattgacgt cggacctggg gccggagaac aaggcggaca aatcgtggct
     3901 gcaggcactc ctgaagaagt agctaatgat gaaaattctt tgacagggaa atatctttca
     3961 gggaaaaaat caatccctgt accaaaagaa cgccgtaaag gtaatgggaa agcaatcaag
     4021 atcactggtg cttcagaaaa caatctgaaa aatattagtg ttgaatttcc attaggagaa
     4081 tttgtagcag tcacaggtgt gtctggttct ggaaagagta cattggtcaa tagtatctta
     4141 aagaaatcgt tagcgcaaaa attaaataag aattctgcta agccaggtaa attcaagaca
     4201 atttccggct acgaaagtat cgaaaagatc atcgatatcg atcaaagccc aatcggccgg
     4261 acgccgagaa gtaatccagc gacttataca agtgtatttg atgatatccg tgggttattt
     4321 gctcaaacga acgaggcaaa aatgcggggt tataagaaag ggcgttttag tttcaacgta
     4381 aaaggcggtc gttgtgaagc ttgtcgcggg gatggaatta ttaagatcga aatgcacttt
     4441 ttgcctgatg tctatgttcc ttgtgaagta tgtcatggta aacgatataa ttctgaaaca
     4501 ttagaagtgc attacaaagg aaaaagcatt gctgatattt tggaaatgac agtagaggat
     4561 gctgtagaat tcttcaagca cattccaaag attcatcgca aactgcaaac gattgttgat
     4621 gttggcttag gttatgtgac gatggggcaa ccagcaacga cattgtccgg tggtgaggca
     4681 caacggatga aacttgccag tgaattgcac aaaatctcta atggaaagaa tttctatata
     4741 ctagatgaac caacgacagg acttcatagc gatgacatcg cccgcttgtt gcatgtatta
     4801 caaagattag tagatgctgg taacacagtt ttagtgattg aacacaatct agatgtaatc
     4861 aaaacagcag attatatcat tgatttagga ccagaaggtg gagaaggtgg aggaacgatc
     4921 cttgcgactg gaacaccaga agaaatcatt aacgtaaaag aaagttatac aggtcactat
     4981 ttgaaaaaaa taatggtata aagaggatag atagctgaga tagttaaaaa aacataaaaa
     5041 aatgaatttt tgcttgtttt attaatatca gtgttctaga atcattacat ggtagttctt
     5101 tctacactga ttggtataga taaaaacttc ctataagttc aatccgttca aaaaagaatt
     5161 gagcaatcgg aggagtttcc tttgattgct tttttcttta tggtatgata ttccttgtgt
     5221 agttggaaaa gaaaaacaag agattatgag taagtgagag ataatgatga acagtattga
     5281 aaatgaagaa tttatcccta tattattagg aagcgacatg aacgtttatg gtatggcacg
     5341 ttcttttaat gaagcatacg gcaaaatctg ccaagcatat gcttcggatc agttagcccc
     5401 tacccgctac agtaaaattg tcaatgtaga agttattcca ggatttgata aagaccctgt
     5461 atttatcgag acgatgcttc gtttggcaaa agaaagatat tctgataaga gtaaaaaata
     5521 tcttttgatt gcttgtggtg atggatatgc agaattgatc tcccaacata aacaagag
//
LOCUS       DAKBVG010000158         5477 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000158 DAKBVG010000000
VERSION     DAKBVG010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5477
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.158"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(<1..284)
                     /locus_tag="OUD09_002119"
     CDS             complement(<1..284)
                     /locus_tag="OUD09_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693359.1"
                     /translation="MCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA"
     gene            complement(851..1786)
                     /gene="whiA"
                     /locus_tag="OUD09_002120"
     CDS             complement(851..1786)
                     /gene="whiA"
                     /locus_tag="OUD09_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HCT9693360.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(1820..2818)
                     /locus_tag="OUD09_002121"
     CDS             complement(1820..2818)
                     /locus_tag="OUD09_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HCT9693361.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(2815..3699)
                     /gene="rapZ"
                     /locus_tag="OUD09_002122"
     CDS             complement(2815..3699)
                     /gene="rapZ"
                     /locus_tag="OUD09_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCT9693362.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(3834..4565)
                     /locus_tag="OUD09_002123"
     CDS             complement(3834..4565)
                     /locus_tag="OUD09_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HCT9693363.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(4569..>5477)
                     /locus_tag="OUD09_002124"
     CDS             complement(4569..>5477)
                     /locus_tag="OUD09_002124"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HCT9693364.1"
                     /translation="FERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQELPFE
                     FPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFIPGD
                     DSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKAFLE
                     KIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFNKPF
                     VETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKDRSI
                     KRWLLMKYMFHNYIPRFKKYFHVKEG"
BASE COUNT         1577 a         1187 c          893 g         1820 t
ORIGIN      
        1 tctgcaatca ccgtatcttc aatcaaaatt ctttcctcat ctatttcata ttcaagacta
       61 aacggaaatt ctccatatgt aatctctggc aatggtggtg ctgtagttct ccaaataaat
      121 acaataactg tgaatagaaa aaatccaacg atcgatataa atgttatgag acctgctcga
      181 agtaaaaccc gatataataa aaatgctgta tttcgctttg ctatatctag aaaaataaaa
      241 agtactgcaa tcataatgat agtaaaagtt gtaaaaaagc acattttttg ccctcccttt
      301 ataaacatcg attctcaaat tctaaaccaa atcttgatag ttcaagttca ggtataacgt
      361 ataccaacca tacatcccaa ttactattta tgaacttatg attatcactg aataaatcac
      421 tccatcccct tgttaaaact tctacattaa ttacacctgg ttgttttcga ctagctaatc
      481 caatgaactt atcaaccatc cttatccacc aaccacgaga atcttgacca gaagtaagaa
      541 aaacagttct caaggttgat cgagcagttc cttccagttt ttaattaatc tccagttata
      601 gataaatact tgatcctatc tatatcagga cttctatatt aagcaaaagt ttatgtataa
      661 aactaaaatc ttcattcagt tgtctccccg ctttttatac gattgtatta aaaaacaata
      721 gaagcctttc aacattatgt tactccagta aagttggaat tatagtttga cggaagaaaa
      781 gcaagtatag cttttaaaaa aaactgatac attcgcatct cctgcttcat gtatcagttt
      841 tttctttgga ttaaactgct tcttcacgca gtttttccgc aaattcattt attttgcgaa
      901 ttcggtgatt aattcccgat tttgatattg cacctgaggg aatcatttcc cctaattctt
      961 tcaagctgac ctctggatgt tgaagccgaa gttctgcgat ttcctgaagt ttttcaggaa
     1021 gggcatgtag accgaccttt gcttcgatca attcgatgtt ttcgatttgt ttagaagccg
     1081 catcaatcgt cttgttcaga tttgctgtct cacaattgac tagacgattg accgaatttc
     1141 tcatatcccg tacgattcga acatcttcaa atcggagcat tgagttggtt gcaccaatca
     1201 atgttaagaa atcagcaatt ttttctgctc cttttaaata cgaaatatag ccattgcgcc
     1261 gttcaagcgt tcgagcatgt agaccgtaat aatttaacat atcgcaaatg tcttggttat
     1321 gctgttcata aatagaaaat atttccaaat gataacgact cgtttccgga ttattgactg
     1381 aacctgaggc cataaaagca ccacgtaagt aagaacgcat tttttgcgaa tttcccataa
     1441 tctcgtccga aacgtgtcca ttgaacatca taccatccat gatatcaaga tccatcaaca
     1501 catcttttgt gccttgcttc aaccggacga tatagacatt gttctttttt aatttcattt
     1561 ttttacgaac gagtagctca cttcgtacct gataatgatc tttcaaaaga gaatagatac
     1621 gtctggcaat tgcagcattt tctgtttgga cgttcaaaat aaactgctgg ttcacaatac
     1681 ctaaagagcc gttcattcgc agcaaagcag ctaattccgc tttcgcatgt tctcgatgaa
     1741 cttccaaacc agttagttct ttcttcacat ctgcagcaaa tgacatccta ttctcctcct
     1801 ttctccaaca ataaattgct taatattttg aaccgaaaac taaacggaac aattcttcta
     1861 ctactttgtc tccatcgtgg aagacaccac catctttcaa ttctaaaaag tcggtggaaa
     1921 tcactcgaca tccttcattt ctcaatccgt tgaagtcgtg tgcgacttgt actagatact
     1981 catcatagat ctctgggtcc atataatctt caggcacttt ttccgtattg accaatactg
     2041 tatcgataaa aggctgattc aaatgcttgt gtaaaacacg tatatgatcg gcatctgtga
     2101 aatgttccgt ttcccctttt tgggtcatga tattacagat atagacggtt tcagcagttg
     2161 tcttgagcat tgcttcgccg atttctggta tcactaaatt tggaaggata ctggtgaata
     2221 aacttccggg tcccaaaacg accatgtcgg cgtccataat tgctgaaacg acttttcgag
     2281 ccgctttggc ttcactttta tcatttgtgt tgcagacata cacacggtca atggttttac
     2341 ggttgatcgc gattttagat tctcctactg cagtcgttcc gtctttaaaa acagcatgca
     2401 aggtcaaagg agtttctgaa gaaggataaa catgtccatc tacatgcatc atttttgcta
     2461 ataattgaat cgcctcataa gtgcttcccc gcatctcgga aactgcggca atgatcagat
     2521 taccgatcgt atgattggct aaaaactgat cttccttttt gaaacggtat tggaaaatat
     2581 cttcatagaa ttgtggcata tccgataacg ccactaagac atttctcaag tcaccaggag
     2641 gagccatagc gatcgattct ctgattgcac cgctgcttcc accatcatct gcaacagtca
     2701 ctactgctgt gatatctgct ccttgatttc gtaaactttt caggataact ggcagaccag
     2761 tgccaccgcc gaccactacg atctttggtt ttcttatccg gtaggttttc attttcacga
     2821 ccggttcacc gtctctttcc ttttatcctt gtcccgatgt gtcacattga ctttatagtt
     2881 tttccctaaa gcatttgcca aacgttcagt caatgccaca gaacggtgtt gaccgcctgt
     2941 acagccgatt gcaactgtca agctgctttt tccttcttta atgtatccag gcaatacagt
     3001 ttccagaaga tccaaaaatt tatggtagaa tgattgcgtt tcatcaaagg acatcacata
     3061 atcatagact ggctgatctt ttccggtcaa tggtcttagt tccgggatat aatgagggtt
     3121 gggcaaaaaa cgcacatcca tcacaatatc cgaatcgatc ggcaacccgt atttaaaacc
     3181 aaatgataca aattcgatcc gaaatccttg gtctcccttt gatttgaatg actcattgat
     3241 tttttcacgt aattgtcgtg gtgtcaatgt tgtcgtatca atgatcacgg aagcttgggc
     3301 tttcaagtcg tctagtatcg cccgttcttt acgtatccct tcagtgatca aaccgtccat
     3361 cgccattgga tgtgctcgtc ttgtttcttt gtagcgagcc actagttctt cgtctgaagc
     3421 atctaaaaat aagatagaag tgtcgatgaa gttcgtattt tcgatttcga tcagcatgtt
     3481 ttggatttct tcaaagaagg aacgagaacg caaatccact cctaaagcca tttttgtgac
     3541 tttacctgat tctttgatca attcccagaa ttttggaatc aatcgaggcg gcatattatc
     3601 gatacaaaaa tagcccatat cttcaaaact ttggatagct actgttttcc ctgcaccact
     3661 catacctgtt atgatgacta attgcaaatt atctgtcata tttacgaacc cctctcacat
     3721 tgtgcataat aaaattatat catcccgggc gtatcattac cagttttctt tttcttttac
     3781 aaaaagattt ggaccaaata tcttttagga tatttgcccc aaatccaatt tttttacttt
     3841 tccgatgctg tatccaatgc ctcgcttctt tcagctaatg cacgttcgat cgttcgatcc
     3901 gctaaaatag attgagcatc gatcactggg taatgattat cttcagcaaa ttcatgcatc
     3961 aaagataatt ctgtgcaacc taggatgact ttttcacagt ttaggcgctc taccgcttcc
     4021 tctaagattt cgtaatagag ctcttgattc aaataatccg attccttgat ctcatgatag
     4081 atcaggtaat tgattttttc ttgaagcgca gtatcaggaa tcactgtttc gaaaccaaga
     4141 tttttgactt cgcgttcata gattccagct ttcatacttc cttctgttcc tagaatcgct
     4201 actctccctg tcgtatgctg acggacaagt tcatttgctg cttctcttgg catatgcaag
     4261 ataggaatat ccgttgccgc ttgtaactct tcaaaaaagt agtgtgcggt gttacaagtc
     4321 aacacaatga agtttggtcg caataaattt tgtttctcaa tatcatccag cagaaacggc
     4381 attggatttt cttctgatcg atctaaaata taagctgtac gatcaggaac tgttgcatga
     4441 ttgaataaga cataattcaa atattcttga tcttttgtag ctttcgttcg gtggttgatc
     4501 aagcgaacaa aactttctgt cgccatcgtg cccattccgc ctaaaatact gaaaaaattc
     4561 tccatgaact agccttcttt cacatgaaaa tactttttga atcttggaat atagttatgg
     4621 aacatatatt tcatcaacaa ccagcgcttg attgatcggt ctttttcata aaatacagta
     4681 gtaccgtaac gattttcttt gatcatttgc tcagctagtt ttttgtcttc attttcccga
     4741 gcatattcta aaaagatttt tttgggcaca cccatccata atcttgcttt atctgattga
     4801 ttcgttccat aggtcgtttc aacaaagggt ttgttgaaca ctcgatcttc tgtgacaaaa
     4861 cgagccagat tcaagccgtt caatgtcaca aagaaactgc tacgaccttg tctcaaattg
     4921 atttcaaata atttatattc tccatctcta ggatcatact tcatatcgaa attggcaaag
     4981 cctgtatatt cgattttttc caaaaatgcc ttaatggttt gatagatttt ttcgttataa
     5041 tctggcatga tcacaacgta attaccaatc gatgctggtg taggatcttc taatagtgga
     5101 tgaccaaggc acatcatccg aacttgatga tcttcatcta catacgcatt tagcacacgc
     5161 atattactat catcacccgg gataaaatct tgtacaatca tttcactttt ataaccggct
     5221 tcgtatatcc ggcctaaaat taaatcgaac tcttctctag tctctaaaat aaaagctttt
     5281 tttcttcctt caaattggac agaaagatac tccacactgt ttgcaggttt caaagcaact
     5341 gggaattcaa aaggcaattc ctgttccaaa tgtccattta ctaacatttc ttctctgacg
     5401 atcaatgttt taggatacgg aagatcatat tcttcacaaa cttcataaaa gcttacttta
     5461 ttgatcagcc tttcaaa
//
LOCUS       DAKBVG010000159         5442 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000159 DAKBVG010000000
VERSION     DAKBVG010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5442
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.159"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(137..1597)
                     /locus_tag="OUD09_002125"
     CDS             complement(137..1597)
                     /locus_tag="OUD09_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693365.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(1805..2731)
                     /gene="glsA"
                     /locus_tag="OUD09_002126"
     CDS             complement(1805..2731)
                     /gene="glsA"
                     /locus_tag="OUD09_002126"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCT9693366.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(2754..4181)
                     /locus_tag="OUD09_002127"
     CDS             complement(2754..4181)
                     /locus_tag="OUD09_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCT9693367.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(4212..5426)
                     /locus_tag="OUD09_002128"
     CDS             complement(4212..5426)
                     /locus_tag="OUD09_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HCT9693368.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         1711 a         1136 c          921 g         1674 t
ORIGIN      
        1 taagtcaata gcattttaaa aaaatttcag agaaagcttg ttatataagt agatcttcgc
       61 acgaatcagg actcaaataa aaaccttcta cagacaatta aaaagctttg tagaaggtct
      121 cctaattaag gtctcatcaa gaaatatgtt tttttaatag gtgaagttcc tgagcaatat
      181 gatacaaatg gatgtttgca ccttttaatg agattggata gatctttgtc ttttcagttt
      241 ctaatccatt ttgatagcta gcagcatcgc acaaaaccac tctttttttc gcacatagac
      301 attcatcagg caattcttct aatgaacgaa ataaaatagg atttatttca gcgatgcctt
      361 cttccgcata caaatgaaat tgggttcgat aaaaaaattg tttgactgaa ctttctgaaa
      421 caacaaaaat ttctatttcc tgtctccccg cttgttcact gttgatcaaa gaaagacttt
      481 tgacgaattt tcgaatgaaa ttttcattcc atcttagttg gatttgattt ttctttttcc
      541 actcttccaa tatttctaca atttttccgt acagaagttg ttctttttct gatagaagat
      601 agatcctttc cggtaaaaat acttgtccat ctgcccacaa cgtacggaca aattgtaaaa
      661 aggattgctt gaaaataaaa ttttctgcat cgtcgatttc agcgatacta ataatctgta
      721 aaatcaattc attcagtgat aggtcattga gaacaaaatt tttataaaca acttcgaaat
      781 cttttcttaa cagctccatg ttctcattgg tataatttct actgttgaac aaagaaaaaa
      841 tataatagac atcttcttct tgtaagtcta gaattccttt ttgctcttcc aatccttgac
      901 agactaattg aaataacggc ttcgtttctg cgcttttctt atcttcttct ttcagtccaa
      961 ggggaaatgc ctttcttccg attgcgatct ctattcctcg tatgataaag gacttttcat
     1021 cttccgaata tcttctttta aaaaaatgag tctctacata atcaatcaac tcgttacaag
     1081 cttcttctac atacggattt ttattttcat agccttccca attgacataa cgtactaaag
     1141 ctagcaagat gttccgcgag tcaaattctg ggataatgat ttctttattt ttcaaataat
     1201 cattttcttt aaaaaaatcc aatacgattt ttttgattgt atatactttg ctcaaagaaa
     1261 taaattcaag atcagccagt tctgtactcg tgaatatacc attaaaataa taacttaaaa
     1321 actttaaaaa ataagattga ccgtacagaa actgtgtgag ctcagaaaga gcaataccat
     1381 gtttgaatac gagtttatac ttatgcttct ctcttacttg ttcttttata caatattcta
     1441 gacactcggc tagactttct agatcgtgaa ggatcgttgg tgtagtcacc cttagagaaa
     1501 tagctgtttg ttcgatgtct atttcttgga attcaaataa ttgttcacac agatacactt
     1561 ttctaaatat atttcgctcg atgtaatttt ccagcattcc tgatacacgc ctccagtttt
     1621 tcttatgatt ttatttaatc atatctgtaa atcgtatttt tgtttccttt ttatttagaa
     1681 aagccctttc aaaaaccaat aaaaaaagaa actgggacaa aagtcaaaca agctcacaat
     1741 ttcagcctca ttgtaggtag ctttgagccg tttggacttt taccccagcc tctttcaaga
     1801 atggttattc aaagatgtca gcgtctagtt ctttgactac atctttcaac aattggatac
     1861 cagctgcgct gttaccaaat ggatctagtg ccgggctgaa gacacctatg ccgtaacgat
     1921 ttggcacagc tgccatcaac ccgccgccta caccgctttt tgcaggtaca ccaacatgga
     1981 cggaaaactc tcctgattca tcatacaaac cagcagttgt cataattgat ttgacgattg
     2041 tagcactttc ttcagtaatg atttgtttgc cattccaagg agcaattcct ttatttgcca
     2101 gcacagcagc taattttgct atgcctgtag cggtgacatt gagggagcat tgtctgaaat
     2161 aagcatcgag tatctccggt acgtctcctt cgatcatttg attacctttc atatagtaag
     2221 caagagagcg attgatatca cctgttcttg attcagaatg atagatttct tcattcaatg
     2281 tgatattagg atcatcacag atctctttca taaatgacaa gattcgctca aatttttcat
     2341 ctgcatcttt accggcaatc aacgaggttg ttgcaatcgc tcctgcatta acaaaaggat
     2401 tcattggatg tttgcgatga ttgatttcca tattcatgat agaattgaag gcaaaaccgg
     2461 ttggttccgt gttgattgtt tgaaagactt tttctattcc gttgtctttg attgctaata
     2521 acaatacggg aacttttgac atgctttcga tggcaaagcg cacatcggcg tctcctccct
     2581 caatcagttg ttttgtatcc aaatcataaa tcgccatacc taattgtttg ggatccactt
     2641 cagctaatgc tggaatgtaa tccgccactt gtcctgtttg ataatagtgg aaattttttc
     2701 caatcaattg ttctaattct tttacttcca tttgaataac cagctttcat tttttatttt
     2761 tctgcatgct ggaatttatg acggcatgcg tagatcgtaa atggaataac taagacgata
     2821 agataagaaa caatcagaat cgtttcataa atcccatgct gtccagctgc gatactagca
     2881 ggtggaacaa atgagataca aaaagcaaaa agtgaagtca aaagacctaa acaagcaacg
     2941 attactttta caacttttcc gcctggaacc tgataggctc tttcaagact gtcatcttta
     3001 aaaaccagct tcaaatagcc cgcgaaaaac ataaagtaca acatcaggta gattacaaca
     3061 gtcaatgaca tcgctgcaag aaatgaaaga ttattccccc cgccgcttaa tgtaaagacg
     3121 gctgcccaga ttgttgcaac gatcccattt gcgatcatca aatgagcagg aacaccgtgt
     3181 gtgttttctt ttgcaaaata ttcaggaagt aatccttctt ctgctgctac gtgaagtgct
     3241 tttgaaggag aggttaccca agaagcaatt tctccgatga cacctaaaca aacaagaatc
     3301 gcaagaattt tcgcaatcca tgcaagattt ggatcaatgt gtcttaatag gaattctaac
     3361 gattgaatca cccctgtatt aagagacaaa ccatgttgag gtactgtagt agcgacggtc
     3421 aatccgccaa aagtatctaa aatgattgcc agaataacca atacaaacat tgctaatgga
     3481 taatcctttt tagggttttc catttcattt gcatgactgg cagatgtttc tacgcccata
     3541 tatgctaaaa taaaggaaac aaaaacaacc agcgtaggca atttcgaaaa atcaggaatc
     3601 aatgttttca tagacagtat cgtttgtagt ggagcgcctg aagcgacata atggatccct
     3661 aagccaaaca agactaatgc tggcaacaag ataccagcta caaaaccgat ccgagcaatc
     3721 aatgaagtgt atttggttcc ttttaactgt gagagtaaaa tagcccagaa aataatcaat
     3781 aggattgcca atttcagata tttattgttt tgtatagcac caatacccgt tgcataggat
     3841 aatgcaccag ttaagaaata cagcatggtc aagtagccaa ctgttatctc aaaccattgg
     3901 aaaaagattg cggcaaaacc gaatcgtttt cccaatgtgc gactgaccca agtatagaca
     3961 ccgccttttt cccatccttt caccgttgcc atctctgctg cgcacaatgc cacagggata
     4021 aaccatagca aaccgcctaa taacaagaaa aatactaagc tgaagcctga ggtcgcgaat
     4081 gtgggatatt catataatga aacgaccatt gagatggtca tagccaaaaa gccaaagaac
     4141 gttaattttt ttgcaggaat tttcgttttg ctttgttgca tgaatcttcc tcttttctat
     4201 ttgaatttgt ttcaattgag tttgttgcca aagaagaaaa agtccaatcc ttcttcttgt
     4261 tcttcttttg tagtagtgat cgttgtaaag tgcatcgccg attttaaaat cactgttgtc
     4321 agcacatagg catacacggc agtaatgatg acaccaatca cttgaatccc taagaagtac
     4381 cagctgcttg gattcgttag taaatggctg ttagatgcaa agatccctat cagaatcgta
     4441 cctgtgaatc cgcccatgcc atgaacaccc cacacatcaa gtgcatcatc ccattgtttc
     4501 tttttacgaa aatcaacagc agcatgacag acgatacccg caatgatccc aatcagcatg
     4561 gcactttttg cttctacata accggcacaa ggcgtaatgg ctgccaatcc agcaacggat
     4621 cctgttagta cgtcgacaaa atcaaagcca tttcctttta cttttgcaat gatcagccaa
     4681 gtaatcatcg caaatgcaag agcgatgaat gttgaaccaa atgcattgac tgcttgttca
     4741 tctgctcgaa gtgcaccacc ggaattaaaa ccgaaccagc caaaccaaag cagtcctgta
     4801 ccgatagcag ctgccataag actgctatgc tgacttcttt ctcctaaagg aatctttctt
     4861 tgaccaagtg tgaggataca tgctaaagag ccgaatccag ctgtcgtgtg gatgactgct
     4921 ccaccagcga aatctctaaa tcccatctga tccaaaaatc cgccacccca tatccagtgg
     4981 caaacaggga aataaatcag cagattccaa aaaataacaa gtataatata gccttttaaa
     5041 ttcaatctcc ctgcaaaagc tcctgtcatc aaaggaacgg taatcacgat aaacatcagt
     5101 tggtaaagaa agaacattaa aaatggaatc gttgcaccat gaaacatgtt ggggaagctt
     5161 gtaacatgtc tcatcaaaaa gaaatctgcc gggtttccaa taacaccgcc gatatcccgt
     5221 ccaaaggcca atccgaatcc accaaatacc cacataatcg ttgttatccc aacggagaga
     5281 aaagattgag acatgatcgt tagtatatta ttcttccgtg ctaatccgcc gtaaaaaaaa
     5341 gctagaccag gagtcatcag gcaaaccatt gctgtacata aaatcatgaa tgctgtatct
     5401 cctgtattga taggtaatgt attcattttt ttcgcctcct tt
//
LOCUS       DAKBVG010000160         5422 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000160 DAKBVG010000000
VERSION     DAKBVG010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.160"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            <1..104
                     /locus_tag="OUD09_002129"
     CDS             <1..104
                     /locus_tag="OUD09_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCT9693369.1"
                     /translation="DKGYGSAIVMFLLVIILIITAIQMKLQKKWVNY"
     gene            120..944
                     /locus_tag="OUD09_002130"
     CDS             120..944
                     /locus_tag="OUD09_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCT9693370.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            976..2286
                     /locus_tag="OUD09_002131"
     CDS             976..2286
                     /locus_tag="OUD09_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HCT9693371.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            2434..3693
                     /locus_tag="OUD09_002132"
     CDS             2434..3693
                     /locus_tag="OUD09_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCT9693372.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            3703..4575
                     /locus_tag="OUD09_002133"
     CDS             3703..4575
                     /locus_tag="OUD09_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCT9693373.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            4587..5417
                     /locus_tag="OUD09_002134"
     CDS             4587..5417
                     /locus_tag="OUD09_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCT9693374.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
BASE COUNT         1771 a          814 c         1050 g         1787 t
ORIGIN      
        1 atgacaaggg atatggttcg gcaatcgtta tgttcctttt agtgattata ttaattatta
       61 ctgcaattca aatgaagttg cagaaaaagt gggtaaacta ttaaggaggc tatcaaatta
      121 tgaaaaataa atttttttca aatgcgccaa tccatatcat gcttatttta ggtggtattg
      181 caatggcatt accttttatt tggatgattt taacttcagg gaaaacgttg gttgaatcta
      241 cccaaattcc acctacaatt ttccctgaga aatttcagct aagtaactat caacaagtac
      301 ttgatttatt accattcatg gctttttata tcaatacatt tttaatggtt gttggacgag
      361 tgattggctc ggttttattt tcagcaatgg ctgcatatgc ttgtgcgcgt ctaagttttc
      421 cagggaaaaa tatccttttc agcttagtac ttattcaaat gatgattccc ggttccattt
      481 ttattactcc acagtattta ttagcacaaa aattgggtat tttaaataca attccagcat
      541 taatattacc aggggtagtt agtgcgtttg gtacgttttt attaagacaa tttttcttag
      601 gtttacctga tgaattggag gaagcggcct atttagaagg tgccaatatt tggacgatct
      661 ttttcaaaat tatgttgccg cttgctaaat caggattggt tgcacttgga atattcacag
      721 cgttgtttgc atttaaagat ttaatgtggc cattgattgt caatacttca attgataaga
      781 tgcctttagc tccgggatta gcaaacttac aaggacaata tgccacgaac ttcccacaat
      841 taatggcagg ttcgttatta gcaatttggc caatagttgt gatattcatc gttttccaaa
      901 gaaaattcat tgaaggaatt gctacaacag gtagtaaatt ataaaactat taattagtga
      961 ttggagagta agtacatgac aaaaattaca tttattggtg caggaagtac tatttttgca
     1021 aaaaacgtat taggcgatgc gatgttaact ccaagtctac aagatgcaca tattgcattg
     1081 tacgatattg atgaaaaacg actaaaagaa tcaaatttaa tgcttaaaaa tattaataaa
     1141 aattcgaatg aaaatcgtgc tgatattgtt tcttattatg atcgcaaaga agcgttaaga
     1201 gaggctgatt ttgttatcaa tgctattcaa gtgggtggtt ataaaccttc aacagtgatt
     1261 gattttgata ttcctaaaaa atatgggttg cgacaaacta ttggtgacac catggggatt
     1321 ggtgggattt ttagaaccct acggacacta ccagtaatgt tagactttgc taaagaaatg
     1381 gaagaagttt gtccaaatgc ttggttattg aactatacaa atccgatggc gatgttaacc
     1441 atgggtgttt taaaagcgac gagtattaag actgttggtt tatgtcatag cgttcaagta
     1501 tgcgtaccag aattgtttga gcatttagga atagccgatc aatatgactt aaatgacttt
     1561 caatggaaaa ttgcaggcat taatcatatg gcattcttgt tggaaattac aaaaaatggg
     1621 gaagatttct atccaacaat tcgtcagtta gctgcagaaa aaccaaatcc acatccagat
     1681 tctgtacgtt ttgaattgtt gaaacgtttt ggttactata ttacagaatc aagtgaacac
     1741 aatgcagaat accatccata ctttattaaa tcaaaatacc cagaattgat tgacgaatta
     1801 gggattccgt tggatgaata cttacgtcgt tgtgaacaac aaattaatga ttgggaaagc
     1861 atgcgagagg acattgtaaa taatggggag ttaacacata cgagaagccg tgaatatgct
     1921 tcatatatca tggattctat tacgacagga actccgtcag tgattgcagg aaatgtctta
     1981 aataaaggct taatcacaaa cttaccgaca gatgtttgtg tggaagtacc ttgtttagtc
     2041 gataaaaatg gcgtgcaacc aacctatgtc ggtgaattgc caacacaatt agctgcttta
     2101 aatcggacga atatcaatgt tcaagaactg acagtagaag ccgctttaac actagataaa
     2161 tcaaaaattt atcaagcagc gtatttagat ccacatctat catctgaatt gtctttaaca
     2221 gatatcaaaa atttagtgga tgatttaatc gaagcacatg gggactattt acctgaatat
     2281 aattaaaaag aagctgaaag agtaaggaat tttcaattcc ttactctttt cttagttaaa
     2341 tttagtaaaa atattgacaa aacacttagt aaacagttac attagtgtta acaaaaagaa
     2401 aacgcattca aattgtataa ggaggagcat aaaatgaagt ggtctaaaaa aattatttta
     2461 gcttgtatag cactaggagc aacgatctct ttgactgctt gtggaaaaaa tcaggaagat
     2521 gcaagcggta aggtagaaat agagtttttt aatcaaaaaa aagaaatgac tcaaacgatt
     2581 caggagattg ctaaagattt tgaagcaaaa aaccctgata ttcatgtaaa agttgtggat
     2641 gtaccgaatg ctggtgaagt aattaaaaca cgtatgcttg caggcgatgt accagatgtc
     2701 attaatcttt atcctcaaag tatagaattg aaagaatggg cgaaagctgg ttatttagag
     2761 gacttaaccg aagaaccgta tttagagaat atcaaaaatg gatatgcaca acgttttgct
     2821 attgaggatc gtgtatatag cataccatta accgctaatg tctatggttt ttactataac
     2881 aaaactgctt ttgaggaaat ggggattcaa gcaccagaaa cttgggcaga atttgaaaaa
     2941 atagtagctg agattaaaga tcaaaacaaa gtaccgtttg cgattgcagg agcagaaggc
     3001 tggactttaa atggttatca ccaactagct atagcgactg ctgctggtgg tgaagaggaa
     3061 gcgaacaatg tttggcgttt ctcagaagtt aatggaatta atgctgaaag caaagaaatg
     3121 caaaaagatt ttaatcgatt agatttgtta agagaaccaa aagctttgca aaacaactgg
     3181 caaggtgccg gttataacga tacagtagtt acttttacaa aaggtgatgc gttgattatg
     3241 ccgaatggtt catgggcaat gccgatgatt cattcacaaa atccagattt tgaagtagga
     3301 acttttcctt ttccagcaga tgaagcaggt caaagtctaa cgattggcgc aggagaccta
     3361 gccttatcag tttctgcaac ttctcaacat aaagaagaag ccaaacgttt tgtagaatat
     3421 atgacaacac ctgaagcgat gcagaaatat tatgatgtag atggtgcacc gtgtgctgtt
     3481 gagggcgtta tcgagaatac tcaggattca ccattaagtg gtttaactga acttgctttt
     3541 actgatcgtc atttagtttg gttagccaaa gattggaata gtgaaaatga tttttatacc
     3601 ttaacaacaa attatttaca caatggcaat caaaaagcca tgattaacgc attaaatgct
     3661 ttctttaatc caatgaaagc tgacggaagg taggaaaata aaatgaaaat aaaagaaatt
     3721 ttaaatcgtt actgggcaat catttttgta gcaattccaa ttattttgca gatgattttt
     3781 ttctactttc caatggttca aggtgctttt tacagtttaa ctaattggac tggtctgact
     3841 tataattttg attttgtggg tttgaacaat tataagattc ttttttctga tccgaagttt
     3901 atcaaatcga ttggcttcac tttaattgtg acgctttgca tgattgctgg acaaattggt
     3961 tttggtattt tgattgcgcg ggcgttgaac gcaaatatta aagggaaaac attctttaga
     4021 gcatggttct tttttccagc ggtcttatcg ggcgtggttg tttctttagt atttaaacaa
     4081 atttttaact atggtttacc aaaaattggt agtatgttag gcattgaatt tttacaaacc
     4141 agtcttttag gaacaacatg gggagcgatt tttgcagtaa tttttgtttt actttggcaa
     4201 ggaattgcca tgccagtcat cattttctta gcgggttttc aaagtattcc acaagaaatt
     4261 attgaagctg ctagaatgga tggggcgaat gataagcaaa ttttttggaa tattgaaatc
     4321 acctacttat taccaagtat ttcaatggta tttattatgg cattaaaagg tggcttaaca
     4381 gcatttgatc aaatttttgc tttaactggt ggtggaccaa gcgatgccac aacatctttg
     4441 gggttacttg tttataatta tgcttttaaa aacaatcagt ttggttatgc gaatgctatt
     4501 gctttagtct tatttatctt aattgggatt gtttcactgg cacaattaaa attatcgaaa
     4561 aaatttgaaa tctaggaggt ttcccaatga aaacatcaaa agaaaaaaat atttctaaat
     4621 acttgctttt attattaggg ggcttcatga ttttagttcc cttagtttca acgctattcg
     4681 gttcatttaa aacaacaccc gatattatga ataatttctt tcgttttcct aatccagcga
     4741 ctttggaaaa tttccgaaga ttgttagcgg atggaattgg tcattacttt tggaattcgg
     4801 caatcatcac aatcattgca gtagttgctg tgacagcaat tattcctatg gctgcttatt
     4861 cactagcaag aaaaatgact cgtgggaaag catacggatt gatgtattta tttttaattt
     4921 taggaatttt tgttcctttc caagtaatta tgattccgat taccgttatg atgagtaaaa
     4981 ttggtttagc aaatatgtgg ggattaatct tactctatct cacttatgcc gtaccacaga
     5041 cactattttt atatgttggt tttattaaac taaatgtgcc tatcagtctg gatgaggctg
     5101 ctgaaattga tggggctagt cactttacaa cttatttcaa aattgttttc ccaatgttaa
     5161 aaccaatgca tgcgacgact attattctta atgctctatg gttttggaat gatttcatgc
     5221 taccattatt aattttaaat cgtgattcta atatgtggac actaccattg tttcaatata
     5281 attatacggg acaatatttt aacgactatg gtccaagttt tgcttcctat acagtaggaa
     5341 ttgtcacgat tacactagtt tatcttgctt tccaaagaag tattatagct ggaatgagca
     5401 atggagcagt gaaataacca at
//
LOCUS       DAKBVG010000161         5348 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000161 DAKBVG010000000
VERSION     DAKBVG010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.161"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            13..2340
                     /locus_tag="OUD09_002135"
     CDS             13..2340
                     /locus_tag="OUD09_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCT9693375.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSATETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2466..2795
                     /locus_tag="OUD09_002136"
     CDS             2466..2795
                     /locus_tag="OUD09_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HCT9693376.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     AEIKTSD"
     gene            2835..3839
                     /locus_tag="OUD09_002137"
     CDS             2835..3839
                     /locus_tag="OUD09_002137"
                     /inference="COORDINATES: protein motif:HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HCT9693377.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKTKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            3836..5265
                     /locus_tag="OUD09_002138"
                     /pseudo
     CDS             3836..5265
                     /locus_tag="OUD09_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
BASE COUNT         1892 a         1030 c         1062 g         1364 t
ORIGIN      
        1 ttgagaggag ggatgaaaat gattaagaaa actcaaaata aaatccaacg tatatgtcaa
       61 aaacattttg tttctagact caaagctgta tctaaaagaa aatacttaca tcccattatg
      121 atttcgatca tcttgatttt tactttctta gggagcttat caagtattta tgtaaacgtt
      181 tatacagaaa cgtcaaattt tgctaaccaa acgacagagg agacaaatag tccaccctat
      241 tctgaaatac agtccataga aagcaccaat cagtctagca acaacgagac aacctcaacc
      301 acatcctcga ctcaactgat tgatccgaca gaaaaagaaa tcacagcagc aaaagcagca
      361 ggggagaaag cttattcaga aacaggtaga agccagacat ttagtactgt tgcggccact
      421 gcgaatttca acggacatac cataccagat ggatatacag tgatatatgc caaagatgga
      481 gtcattgttg cggttaacgg atcgatgagt gcggataaaa ctgcgttaca aggcgtaacc
      541 aaagcaatcg cttttattga taaccgtcca gatatccata atctgtttat tggctccaca
      601 gtaaatggta ctactatcac attcccaaaa agtatctata tgggggtggc taccgctgga
      661 aatacgatcg attcagttac acccaatgcg gataaaagtt attcttttac accctcaaca
      721 tggggagggc ttcctgccgg aggaggtatg agtacttttt caatcggtat acagttagca
      781 aatgatattc cattcacggg gtactcgatc aaaaatgggc agatcgttga tggtaaggga
      841 aatgtttcaa attttccaac ctatcaagca gcaacggttc aggtcaatgc gaatcggaca
      901 ggaacttttt cttatacagt caatggtgga gcagcaaaaa tcggaacgta cgggactaca
      961 ttatctggaa tccatgtggg tgacaaagtg gttatcactc caacagcagt aacgggctat
     1021 tcttttgcac aaacaaacgg ttctttcaca gccctagaaa gtaacgtcga tacggtgacc
     1081 tatacaggca aacaaacacc aatcactatt aagtatcaag actattttgg acagatgatt
     1141 gctccttcta aaataatgaa tcttacttac ggaagtgctg cacaagacct gacgactaat
     1201 acgcctacta tcaacggata tacctttaca gctgtttcag cgacagaaac aaagaaccaa
     1261 tcagcaatga gtgttttagc tagtttagat acaaatggaa atgtaatcgt aaaggacgca
     1321 aatggaaaac agatttctga agtcatccta tattataaga caaatgcaac agtatcaatc
     1381 aatgcaaatg gcagtaagta ctatgatggt ctttctgttg taccgatcgt taaatatgcc
     1441 tttaatgata aaacagaatc aacatccctt ttaaacaatg atctcagtat tgatcaaatc
     1501 acatggaatc caactgattt caaagcgatg aatgaaaagg gagttgaagt aactgcgcct
     1561 actgaaattg gaacctatac gtcttggcaa ttgacaaaaa gcggactagc taaactagct
     1621 gccagaacaa actatttatt tactatcgtg cagacatctg atgtctatac gattaagcaa
     1681 atcagcggtg cggtagagct cagtggcagt aagacatatg atggaaaagc aggtgtcccg
     1741 tcaatccatg tcaagctagc agagggtgtc actagttcgc ttactccagt acaagtggca
     1801 cttagcagta tggattacac agcagatgca caatcagcaa aaaatatggt taatgctggc
     1861 agctatacaa ttaaactaac gaatagcgga atagacaaag tcaaagcagc aaatcccaag
     1921 ttgtcattta ctgatatagc caacacatca ggaacttata ccatctataa agctaacgca
     1981 gtgattactg tcgatgacgc tgcctttaat tatgatgccc aaagtcactc gatttcggtt
     2041 ggaaatctcc acgttaccgg tgttgtctta ggcgaatcat tggattatac cctaacagtt
     2101 aacagccgga cagatattgg atcacaaata gtaacgatta gtttaggcga aggaagtgta
     2161 aacaataatt atacgattat cgcaacagat accgctaagt tgacgatcaa tccgactctc
     2221 ttgcttcctt cgacaggtgg gataggtcta ctgccctttg tctttttagg actgatcttt
     2281 attttttctg gcttctttta ttttatccat cgtaagaaaa aagcaggtga acagcgatga
     2341 aaaaaacaaa aaatgtacgt gtggatatat ttgctgttct cttttttatc ctactggtct
     2401 tttttatagg ttctccgact gtgtcagcta gagaatcacc tattacattt actttgcata
     2461 atcgaatgtt cgaaagcgat aagatgccaa gtatcagtca aaacgacgga ataaatatcc
     2521 cggataactt tcaaggagta aaagggttga acggcgttac tttcgctata tatgatgtct
     2581 cggatgaatt ttacaagctg cggtcagaag gaagtagtgt ggaagacgca caaagaaaac
     2641 tagcacaaaa aagtgatagt gaaaaaatac tggcagaaaa tgtgacaaaa acagtagacg
     2701 aggaagaggg cattgcttct tttagtgcat cggacaaaga tgagcaaggt cgtgacgctg
     2761 tttaccgatt tgcggaaata aagacttcag attaagtaaa agaaaaatca gcaccatttg
     2821 tcgttgtgct tcctgtgaca gattcatcta atcataaatt gacgaccatc cacttgtatc
     2881 caaagagcga acaaaaaata cctgctgcac tcacattaac aaaaacagta gagaataagc
     2941 agacagattt tgcagatggg gataaagttc cttatctcat caccgtaaca attcctgaga
     3001 acatcaacga gataggaacc tatacgatca aagatacagc tgatcctcag ttatggctag
     3061 aaccagaaac gatcgatttt ttgattggtg gagacaagat tcatacattt cacacacaaa
     3121 aaacaaaaca tggttttgtc ttatcagatt ctggaaaaga tcttagttcg tttgctggga
     3181 aaaaattaac gatccgttac caaatgacat tgaaagaaaa cagtgaaaaa acgacatttg
     3241 ataacgatgt acgtttgacg actgacaatc aaacagtaga aacacatctt gccatccaaa
     3301 caggaggcaa acaatttgtc aaagtggatc ggcaaacgaa acaaaaacta gcagatgctc
     3361 aatttctagt caaaaaaaat gaccaatact tagcgcaaga aaacgggata aatcattggg
     3421 tagccaaaga tgattcaaga gcaacgattt ttacttccag taaagatgga acattttctg
     3481 tccatggatt gtcttttgga agttacgaac tagtggagat taaaccgcct aaaggatata
     3541 tattgagtca atcaaccatt ctctttgaag ttgaaaaagg cagttatgat ccgcttcatc
     3601 ctataccatt ggaaatcatc aacgaaccaa aagcaaatcc gacgaataat ggaaaaacgc
     3661 cagttcagat gcagacaaat ggtccatcaa atgaaaaaaa tggaggaagc tttccaaaaa
     3721 caagcgaaga gatgcttgga ggtttctcaa ttttagggtt gttgctagtc ctttcaacag
     3781 ggacagcttg gttttataaa aaacaacaaa aaggaaacaa taggagggaa ataaaatgaa
     3841 aaacatcaaa acaaaaatat ggacgttttt aggaactgct atcatgctgc ttcctttcgt
     3901 tctcggccta ggaactgcag aggtttctag gccgtatcac ctacaccaga aaatgttacg
     3961 gttaatttgc ataaattgaa attcacttca gcaccagaaa atcaaataaa caacggaacg
     4021 gaattgacat ttccgaatag cgaaccgttg aacggcgttg aattcaacgt atatgacatc
     4081 actgctacgt actatccaag taaagacaca gcagttccag cagacgctac acccttcgca
     4141 agtgtaacga cttctggtga aggattagct aacctgactc ttccaggtaa atctgatggg
     4201 aaagatgctg tatacgtctt tgtagaaaca ccaaagcccg gtgtcgaaac gtcgccaaat
     4261 atcgtgttaa gcttaccctt ttataacccg aattcatcag accctgacaa acctttagac
     4321 acgattcacc tatatgctaa aaatgtcatc aaagaaagca atatcaaaat ccagaaagct
     4381 gggaactttt caggggtaga taagttagca ggggcgcaat tccagatcaa aaattcagag
     4441 aatcaattcg tgaccggttt tgatgaaaac actggtgtcg ctctctatga tcctgctgag
     4501 tcagcgttga cattcacaac tgatactgaa ggatccatca gtgtcaatgg attaatagac
     4561 ggcaattata ctttagttga aacaaaagca ccagatggtt atcaattaac caatccacaa
     4621 aaagaaacta cttttatagt aaaaaacgga aaattgcaac aaacaaccgg tatcatccat
     4681 ggcgcagatg aagccaatct tgggaatcca aacggtgtag tcaatcggga aaatacaact
     4741 acggataaca cgattactgt agaaaatgtc caaaactacg gtgatttcca cttcatcaaa
     4801 caagatgtga acacgcaaga aaaattagcc gatgcagaat tttctattct aagagcaaaa
     4861 gatccaactg ttaaattaat gaaaaaaata gcaaatcttg cctcaggaga atacccatat
     4921 gcttggtcag atgaagtcac aggttctgac tgggaagaag tgaaattgat ttctgattca
     4981 gaagggaaat tttcaatcga agggctgaaa caaggggcat actgccttca agaaacaaaa
     5041 gcaccagaag gctacgcatt acctaagaat gaagctgcgt acactgaatt tacagtagat
     5101 gatgatccaa caacttctga tgaaacaacg atcgataacc aacctaaagg aatcttgcct
     5161 tcaactggtg gtatggggat catcgtattc atcttggtag gtactgcttt agttggagga
     5221 gcagtgatct acttcaaaaa acgccatcaa gaaacagaag cttaaactat tggaaacaaa
     5281 aatcatacga taaataaacg atagtgattc aaatggggag aaaggttgtg acaagtggtc
     5341 ggtctctt
//
LOCUS       DAKBVG010000162         5338 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000162 DAKBVG010000000
VERSION     DAKBVG010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.162"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(1..127)
                     /locus_tag="OUD09_002139"
                     /pseudo
     CDS             complement(1..127)
                     /locus_tag="OUD09_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390190.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
     gene            complement(480..1169)
                     /locus_tag="OUD09_002140"
     CDS             complement(480..1169)
                     /locus_tag="OUD09_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HCT9693378.1"
                     /translation="MESEDLLSRISEVHPNQVWLDKEKVEKRYIYLFFKRMIDIVGSI
                     IGLILASPLMFYVAYRIRKEEPGSPIFFCHTRVGKNGKTFKMYKFRSMCLDAEEKLEE
                     LLDQNEIEGAMFKMKEDPRVTKIGKIIRAKSIDELPQLWNVLKGDMSLIGPRPPLERE
                     VAEYTRYDLQRLYVKPGCSGLWQVSDRNNVSFHKMVELDLEYISHRSLMYDLRLILKT
                     FYVIVKPNGAY"
     gene            complement(1214..1978)
                     /locus_tag="OUD09_002141"
     CDS             complement(1214..1978)
                     /locus_tag="OUD09_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HCT9693379.1"
                     /translation="MIDLHCHILPGIDDGAENIDASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIDRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEQLFFELLQLGKTPVIVHPERNAHFRKDPNHMLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKMMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(2006..2704)
                     /locus_tag="OUD09_002142"
     CDS             complement(2006..2704)
                     /locus_tag="OUD09_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HCT9693380.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENMSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(2717..3496)
                     /locus_tag="OUD09_002143"
     CDS             complement(2717..3496)
                     /locus_tag="OUD09_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HCT9693381.1"
                     /translation="MEETISLKEIFETLRKHVITILISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQATSTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAPTQPKATNDKTAPSRRSRSRV"
     gene            complement(3512..4456)
                     /locus_tag="OUD09_002144"
     CDS             complement(3512..4456)
                     /locus_tag="OUD09_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCT9693382.1"
                     /translation="MAKRRRRSRGKKIFLIILAILVVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGHDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYRDAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(4497..5276)
                     /locus_tag="OUD09_002145"
     CDS             complement(4497..5276)
                     /locus_tag="OUD09_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693383.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQHHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSLIEQIRNEKIRAAYASKAETVAQQMGYSNTSY"
BASE COUNT         1380 a         1120 c          944 g         1894 t
ORIGIN      
        1 tcagaataat tacaatattt tgggaccgga taactccctt ttgctcgtaa ccactcatat
       61 ccgttaggat tcactaaaag tttaccgttt atttttcgat tctttttttg aaccgtccca
      121 tgaacatacc gattcaacaa gctaaattat aaaaattggt gtgctgtcat tattttttgg
      181 aaaatttatt aatggctatc aatatacttt tgtatcatac taaatagctc gtcagttcct
      241 atatcggata taccaatatt aaagcattta gatcattata tttaagagtg gtatcatccc
      301 aaaatttcga atattttctt ttatataaga ataatgatac tgaattgccc cattcacctt
      361 tcttttttta gattttctac aagtgattca aaccctccat aattacagtt attacttttg
      421 aaacgataat atgaacatgt gtaatctttt ttattaacaa tccatcattc tatccctaac
      481 taatatgctc cattgggctt gacaataaca taaaacgttt ttaaaatcaa cctcaagtcg
      541 tacattaaac ttcgatgact aatatactct aaatccaact caaccatttt atggaaacta
      601 acattatttc tatcactaac ttgccacaaa cctgaacaac caggttttac atacaagcgt
      661 tgaagatcat atctcgtgta ctcagctact tccctctcca aaggtggccg tggaccaatt
      721 agactcatat cgccttttaa aacattccat agctgtggca attcatcaat gcttttggca
      781 cggataattt tacctatttt agtgacacgg ggatcttctt tcatcttgaa cattgcacct
      841 tctatttcat tttgatcaag taattcttcc aatttttctt cagcatctaa acacatcgaa
      901 cggaatttat acattttgaa tgtttttcca tttttaccaa cacgtgtatg gcaaaaaaat
      961 atcggacttc ctggctcttc ttttctgatt cgataagcta catagaacat caaaggagaa
     1021 gctaaaatca aaccaataat gcttcctaca atatcaatca ttcttttaaa aaataaatag
     1081 atatatcgtt tttcaacttt ttctttatcc aaccaaactt gattaggatg tacttcacta
     1141 atacgagata ataaatcttc actttccata ctttccctct ctcctcattt cctttttatc
     1201 tacgaataaa gaactaaaat aaaccaaact tcttcttttt tacctcttca tagactggat
     1261 aactgatttc atccccgttg atcagatcct ttgcgacttg tttcatctgt actacttttt
     1321 ccttaccaaa gtccttagca atctgctcat agcactcttt taagtaaaag gtacgcttag
     1381 agaccccatg ggcatcagaa gcagccatct gcacaagatt atgtttgacc atcattttgg
     1441 ctgttttctg gatgtctttt ccaaatacac cgacataact tggtgcggtg agttgggcca
     1501 agcatcccat atctaggaat ggcagcatat gattcgggtc tttacgaaaa tgcgcgttgc
     1561 gttctggatg gacgatcact ggtgtctttc ctagttgtaa caactcaaaa aagagctgtt
     1621 ctgcatataa cggcacttcc agtgtcggaa attcgattag aagatatgta tcatctaaat
     1681 ctgtaaataa aatttcatct cggtcgatgt cttcgatcag tgttccggta atacgtactt
     1741 cttgtccttc caataacgtc aaaggcaatt gtcttttctc taattctgct tgtaacgaag
     1801 cgactagaga aatgacttgt gatttttcat tgctgtattt tccattatta tgatggggtg
     1861 tgcataaaat atgtgtgatc ccttgttgaa tcgctttttc agccatggcg atactggcat
     1921 ctatgttttc tgctccgtca tcgattcctg gcaggatatg gcaatgtaga tcgatcattt
     1981 ctcttgtctc ctctcttttt atttcttaat tcccgtagta atagccagca tctttgctgt
     2041 gttctgcccc attgtataca acaccaagga ctcgcgcttg taccatatcc agcaattctt
     2101 ttgctttagt caatgattct ttccgtgaca tattttcacg tacgaccaat aaggtgccat
     2161 ccgttttgga agacatgatc tgtgcatctg ttacggcaac tactggcggc atgtcgaaga
     2221 tcactacatc aaatagttgt cttgcttctg ctaaaatctg atccattctt ggcgaactta
     2281 gtagttctga tggatttggt ggtttaggtc cacttggcag aatcgacaaa ttctcgactg
     2341 gtgttcgttg gatctcatct gctacacttc ctgatgaact aagaacggta cttaatccgc
     2401 ttgcgttgtt caacttgaac gttttataga tgacgggctt acgtaagtct gcatcgacta
     2461 gcagcacccg ttgaccagat ttggcaaaaa caacggcaat gtttgctgca gtggtcgatt
     2521 tcccttctcc aggtcctgat gaagtcacaa caatcgtttt aatttgctga ccagctgcgg
     2581 aggcaaactg gatattcgtc cgaatcgtac gatattgttc agcgattggt gaagaggggt
     2641 ttgtgagtgt gaccaaactg actgcatgag tttgggctgc tttttgtttc tgtgttcgtg
     2701 ccatttttct ttcctcctag actcttgagc ggcttctgcg tgatggcgct gttttatcgt
     2761 ttgtagcttt tggttgtgtc ggagctggtc cttttttacg gataccagca ttcaattctt
     2821 tcgctgacat ttctggtaca gtccctaaga tcgggaagcc caatgtatcc gtcacatatt
     2881 tttcatcttt tactgtccgg tcgagtaatt caaataagaa agctagacct acgcctacca
     2941 tcattcctaa cactaagcca atcactagat tcaatttatt atttggcgaa acaggtgtcg
     3001 tattggcaac tgcacctgag atgatcgaga ttttatcgac attcatcacg tctttggcgt
     3061 tctcttggaa tacttgtgac gttgtattag cgatttgttg agccgtcact gcatttgtgc
     3121 ttgttgcttg gatcgagaac atttgtgaat tttgtgactg attgacggag atggcatctt
     3181 tgatttgtcc agcagtcatt tttacattgt cttctgtttc taaacgatct ttgacttcat
     3241 ttagtactag atcactgacg atcatatcct tatacgtatt gatcatttgc aggttcgtat
     3301 tgacatcatt gacatttgca gtatctgatt gcggcagtgt cacgatcaat tgggcttgtg
     3361 agctgtattt tggcgtcaag atgaaaaacg tcacgatgcc tgagatggcc aagccagcaa
     3421 acattgagat gaggattgtt atcacgtgtt tgcgtaatgt ttcaaaaatt tctttcaagc
     3481 tgattgtctc ttccattttt tctccacttt cctatttatt ctctagattc atttgatctt
     3541 ttaattcttt tttcacacga tcaagttctt gatcgctgac tttttggtat gagatattgt
     3601 cttgcatgaa gccttctcct tgcagctgat cttgcttgat cgtaccaaat gcatcccgat
     3661 aatctaaagc aatctttttc atttcatcaa agctcatgtc tgttttcata ttcgactcta
     3721 gagcattcaa aacggattga tagttgctta caccgttcag actcaacact tttttcgcta
     3781 caccttctac gatctttctt tgacgttctt gtctgccgta gtcaccattt ggatcttcat
     3841 aacgcatacg agaataagac aatgcctgat ccccgttcaa tgtgatttta ccgatcgtaa
     3901 aatcatagcc atcttgtgag aaggtgaggt cattattgac ttcgatacca ccaactgcat
     3961 caacgagttc ttttagacct ttcatattga ttgaaacata gtgatcgatt gggatgtcta
     4021 catatttttg tacggtatcc atcgccatgg cagctccgcc gaatgcgtaa gcgtggttga
     4081 tcttgtcttt tgtgtcatgt ccaacgatgt cgacatacgt atcacgagga atgctgacaa
     4141 tcgttgtctg tttgtcttca ggactgactg tggcaaccat catcgtatct gatcttcctt
     4201 gatcgacccg acctaaatct cccgtatcaa tccccatcaa tagaacagag aaagattctt
     4261 gtttgtccag atcaactggt tttgttcgtt ctccttgggt tcgttccact gattcgtaag
     4321 ttttttcaat agaatgtgag agatctagat ataattttgc tcctactcct acgattgcta
     4381 atactaatac gacaagaatt gctaaaataa ttagaaatat ctttttccct cttgatcttc
     4441 ttcttcgttt tgccatagtt tcctccgttt gctagccttg cttgaattct cgcttattaa
     4501 tagctcgtat tgctatagcc catttgctga gcgactgttt ctgcttttga cgcgtatgcg
     4561 gctcttattt tttcattccg gatctgttcg atgagactga gtgcggtcaa atattgatcg
     4621 taagtagcag tagctgttac ttgaccgtct ttcagcatcg tatcgaatag ctgatctagt
     4681 tgcgtgactt gtgcaatttg ggtatcagcg aatcctagat attgcataac gaccgttttc
     4741 caagcactat ccttaaaaaa gctcatattt tcacgtacat gggcgacatc tgcacttgcc
     4801 aacttttctt tgatgatgac atcatccaca gcttgctgcc agtttgatac gtttttagta
     4861 aataatttat tgacagccgt ttggatcttc agttttgatt ctgcatcctc taactgtgtt
     4921 aagactgctt tcttttcttc ttggatcgca gcagcttctt taggtaattc tgattccttg
     4981 atgtcataat catctgctga aagtttgata gaagatagtt tatgtgacaa atttgaaact
     5041 gtttcttctg ttatttcagg atttaggaaa attttttctt ttgtactgta cagcttatct
     5101 acttcttttg ataaagaggt cacggtgtct ttttgattgg tgatggcaga ttctgcgtaa
     5161 ttgatccgtt gatgatgttg ccgctgatta ttgagatggt atccaaacaa gcaaatcaca
     5221 atagcgcctg tgcctaccgc cacagtagcc aataacttga cgaacggctt ttccatagat
     5281 tcactcctaa atattgatga tagataaata aagatgtgat tcattcacat cagaatga
//
LOCUS       DAKBVG010000163         5244 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000163 DAKBVG010000000
VERSION     DAKBVG010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5244
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.163"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(97..759)
                     /locus_tag="OUD09_002146"
     CDS             complement(97..759)
                     /locus_tag="OUD09_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HCT9693384.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(769..1584)
                     /locus_tag="OUD09_002147"
     CDS             complement(769..1584)
                     /locus_tag="OUD09_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCT9693385.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(1846..2289)
                     /locus_tag="OUD09_002148"
     CDS             complement(1846..2289)
                     /locus_tag="OUD09_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HCT9693386.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(2423..2761)
                     /locus_tag="OUD09_002149"
     CDS             complement(2423..2761)
                     /locus_tag="OUD09_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCT9693387.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(2749..3126)
                     /locus_tag="OUD09_002150"
     CDS             complement(2749..3126)
                     /locus_tag="OUD09_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCT9693388.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            3350..4543
                     /locus_tag="OUD09_002151"
     CDS             3350..4543
                     /locus_tag="OUD09_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCT9693389.1"
                     /translation="MTTYQRKLLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(4709..5131)
                     /locus_tag="OUD09_002152"
     CDS             complement(4709..5131)
                     /locus_tag="OUD09_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCT9693390.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
BASE COUNT         1594 a          992 c          895 g         1763 t
ORIGIN      
        1 ttcatttgtt tagccatatg tttcaaaaaa tgaaaaagaa tgacagtttt tacataaaaa
       61 ctgtcattct taattgaaag tttcaattta taaagtctac agatccatag ttctcatcaa
      121 tttacgattg ttgaagtaaa tcaaaccaaa tacgatagaa ggacctagta gaatacacca
      181 ttggggcttt acgaaaaaga agagaaacaa acttgtcaat agtaaaaaat ctagtaaact
      241 cacataccat tttttcaacg tatagtagaa cgctatccgt agaacttgat ttggtttcga
      301 cgctggattt ctcacctgaa aataccagta gttgatgcta aaggaaactg ataaacaagc
      361 aagtaaaaca aacaacggaa tcaaccattt tccataagaa aaacgtataa aaaagaaaca
      421 atcaaaaatc attatcacac taactatcca agcaaatagc caataaaaaa atccctttag
      481 ccaaaattgg cgaaacgtct ttatgaacaa acgtactggt gaaaaacttt tttcttttgc
      541 taattcttct gtcataccta tcatcacgat gcaacctgga ccaataaaaa gtaaagaaaa
      601 aataaaggaa aagacattcc ttccatctat cgacaaaagg aacgcagctg ctatatacaa
      661 gatattgaca caccaaaaac aaaggctact tgccaatgat tgatacaacc aataaaagaa
      721 ttccatataa acatttttgt caaaacgctg ctctttcata atcacctcct attccttcat
      781 tcctgatgaa gcgatccctt ctacaaaata tttttgcatc gctaaaaata taaaagcaac
      841 aggaagaaca gaaacaactg aggcagccat gattaatgcg tactctgcat caaatagccc
      901 aataaacatt ttcaacccta actggattgt tttattttcc gtagaagaca aataaatgaa
      961 tggccccata taatcgttcc atgtattaac gaatgtgatg atcgtcaaac tggcaatcgc
     1021 cggttttgta agaggtaaaa tgatcttgcg atagattccc cactctgata agccatcgat
     1081 ccgcgcactc tcacataaag aatctgggat actactgtag tattgcttca acaagaacac
     1141 accaaaagca ttgaacgctt gtaatagcac caacgaccac aacgtatatg ttaaacccac
     1201 ttttctcatc ataatgaact gcgggatcat ataagactgc caagggacag caatcgtgcc
     1261 gatatatgct aaaaataaaa tatcgcgccc tttaaaattt agtttagaaa acccgtaagc
     1321 agcaaaacta gaagtaaaca actgaatcaa cgtgatcaca acgcttaaaa aagctgtatt
     1381 cttaaaaaat gtcactaaag gaatacgaga ccaaataacc agataattct ctaaatgcca
     1441 agttttaggg atccattgaa tcggaatcgt gaacacttca ttatttgctt tgaatgaagc
     1501 agaaaccatc cataaaaaag gaatgatcgt caataaagct aatacaatca gcagaatcat
     1561 acccaaccat tgaataaagg gcatgttttt tgaacgaatt tttgcttttt ttctttcatt
     1621 tcgaacacct ccaaagttat aatcttcatt tctttgtaat gattacaatc cagaacaacg
     1681 gttattatag ctgattcatt tccgttcttc ttatgtttgt aagcgcttat ttaacaagtt
     1741 atgtaaatct ctttattttt tttgatttaa aaatgaaaaa atacaaaagc aagactgtca
     1801 tacgtctcgc ttttgtattg aaatttcttc gattagagat acttattact gttcaaaagc
     1861 ttctttgaaa cggtgaatca attcattgac tacaggtgat aaaatccgat tctttttcca
     1921 gactagcatg cagttcgttt caatcattgg gtcaagcgga acgaatgtca ctcgctttga
     1981 aagatgactt acagcacctt ctatgactaa agcagcacct acctgattct caattagcgc
     2041 aaatacattg aaaatcaaat tgtacgttcc tgtgatcagc aactgctcct tttttatgcc
     2101 taaccagtca gcaaacattc gctgtatttc tggacgcttt gagatcaaaa gaggtacatt
     2161 cgcaaagtct tcttttgtta tcacgtcttt ttttgctaaa acagaatttt ctaaaatgag
     2221 caatccccat ttttcctttc ttggtaacaa aatcgtttcg tatttctctg tatctactgg
     2281 atctagcaaa atcgctaaat ctaacaggct tttttctagt cactccgtga tatcatcact
     2341 tgttccgctg tggatatgaa attgaacgct gggataatca cggatcaact tttctagtaa
     2401 tagtgccaat gtattcgaat tatcagcttc cacacagccg atcgtaaatc ggccgctgaa
     2461 taattgtttt tttctgttct cgaattcttg agtggtttga ttcgttaaat ctaaaatttc
     2521 ttctgctcgg cttttttaga aaataacctg cttctgtcaa gatcattttg cggttttcgc
     2581 gtacaaacag ttctgttcct agttcctctt ctaactcctt cagttgtcgg cttaaggttg
     2641 gttgtgtaag atgtagaact tctgcggctt gagagattgt tccttcttct gcaattgtcc
     2701 aaaaatattt tagtaagcgt aactccatag cgatgctcct tgattttttt acttgataca
     2761 ttccatttta tttctagata acacacgatc atatttcttg atttttcctt gtaaacgttc
     2821 caaacattct tgcatttcag caattttttt cgtcaaaagt tcactttctt caatcaaaag
     2881 cttacgccgc tcaaatacag ttgcttctcc ttcgctagat aggcgaatat attcaatcaa
     2941 agactcgatc gataatccgg catttcgcat acagatcgta aagtcgaccc attttaggtc
     3001 ttcttctgta taatcacgga ttccgccttt tgtccgatta actggtggaa tcaacccgat
     3061 tcgttcataa tagcgcaatg tatcggccga aatgcccttt tcttcactga cttgtttgat
     3121 attcatctga tcactctttc tcttaatttc ttgccacttt acttatgtat tttatttatt
     3181 ctacaacttt aagtgtactc caagtcaata ctcatttcta ttaaaactaa gctggattat
     3241 ctatatcggt tgtttgtgta ttatttcaaa taaaaaagtc ttgttttttt attctttttt
     3301 ggatatactt ttaacaattt agggaaattt taggaaaagg ggtataaaaa tgacaacgta
     3361 tcagcgaaag ttattattat cgacatcggc gggaattgct ttagagaata tggatattat
     3421 gtttttagct ttttcattat cttcaatgat tacttctttt catattagtg gtacgcaagc
     3481 gggtttcatt tcaacgatca ctaatttggg gatgctcatt ggaggtattt tttttggcat
     3541 catagctgat aaatatggac gtgtgaaagt attttctcaa acggtccttc tgttttcgat
     3601 tgcttcttta ttgatgtatt ttgcttcaaa catctacttg gtttatctct ttcgctttat
     3661 cgcaggtgta ggagcgggag gagaatacgg ggcgtgtatg tccttgattt ccgaaagttt
     3721 ttcaaaaaaa caaattggac gagcttcttc ggttgctggt attggtgcgc aggtcggtgc
     3781 ggctttggct gcgattttag ctgctgtgat cattccaacg tttggttgga agatgttgta
     3841 tgtcgtaggt gtactgccgg tgttgatggt cttgttcatt cgtcgcggct tgactgaacc
     3901 aaaggaattc aaatctgtac aatctgccga aaagaaaacc aaacttagca gactgtttga
     3961 atcaaaacat cttgcttggc aaacatgtgg ccttagtatc atggtaactg tccaaattgc
     4021 cggctatttt gggttaatga attggctgcc ttcaattatg caaaatcgat tagggctttc
     4081 ggtttctggg tcttcattgt ggatggtcag tactatttta ggcatgtctt tggggatgat
     4141 gacttttggc ataatcatgg ataaattggg gcctaaacta gccttttctt tgtttttaat
     4201 ttgttcatcc ttatctgtct atctacttgt gttagcccat agccagtgga gtttggtagc
     4261 agccgctgtg atagtcggct actttatcaa tggcatgtat ggtggatatg gcgcagtgat
     4321 tagtagtttg tatccaacag aaatccgcgc gacggcgaat aatttcatta tgaaccttgg
     4381 acgagctgtt ggtggttttt cttctatcgt catcggtttc ttgatggatc attatagttt
     4441 aactgccgta atcttgtttt tgagcggtat ttacctagtc agtctgctgg tcttgacaac
     4501 tcttactggt gttacagcat taagggaaag cttgcagcat tgatagttga gtctgtaaat
     4561 gacaagaaag aggtgtggca aaagcttgca tctgcggtaa taagaccagc caaatcaaaa
     4621 acatgagaca ctgtttccgt ttggctggtc gtattacttg atgcagggca gctttttcaa
     4681 ctgctaattt ccttctatct ggcggctact attctttgac gtccgtcatt tcaaagtact
     4741 ttactaataa cttagcataa cgaaaaactg tggatacact cgtatattga gcgtctgcat
     4801 aatcttccaa atttttctca ggatgttgat acatgaattc aatcaaatga taattatatg
     4861 attttctgta gaaatgaaga taaataatgt agattgggaa gttctcctgc ttttccatag
     4921 aaaagcgccg attattctca ttcttcaaaa tgaccttatc aggatatttt tggaacaatt
     4981 cttctaaatc tgttagataa ttcaagatag acttacttga taccttcaat tcgttttgta
     5041 gttcttctat tgaacaccaa cgttctgttt caagcagtaa atgaatcatt ttaattttct
     5101 tttgagaaac ttttgttaaa aaagtctgca tattttctcc tcctgtatct actttactat
     5161 actacataca agaaaaaaaa cggatacaaa tatcatattg tcacaccgtc aatagtgcat
     5221 aaacctgtta ttatttctct tttt
//
LOCUS       DAKBVG010000164         5232 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000164 DAKBVG010000000
VERSION     DAKBVG010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.164"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(266..466)
                     /locus_tag="OUD09_002153"
     CDS             complement(266..466)
                     /locus_tag="OUD09_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCT9693391.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(771..2003)
                     /locus_tag="OUD09_002154"
     CDS             complement(771..2003)
                     /locus_tag="OUD09_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCT9693392.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            2257..2826
                     /locus_tag="OUD09_002155"
     CDS             2257..2826
                     /locus_tag="OUD09_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCT9693393.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(3004..3444)
                     /locus_tag="OUD09_002156"
     CDS             complement(3004..3444)
                     /locus_tag="OUD09_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCT9693394.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            3602..4366
                     /gene="map"
                     /locus_tag="OUD09_002157"
     CDS             3602..4366
                     /gene="map"
                     /locus_tag="OUD09_002157"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCT9693395.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            4398..>5232
                     /locus_tag="OUD09_002158"
     CDS             4398..>5232
                     /locus_tag="OUD09_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HCT9693396.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EY"
BASE COUNT         1606 a          958 c         1019 g         1649 t
ORIGIN      
        1 tttccttcca tcagcataaa agttcacctc aaaaagagta tacgaacgaa tgttcttttt
       61 gtgaaggcgg aatttaatat actgaataat agaaaacaag tacttgtgcc aaattatgtg
      121 ccaaaaaaat tcgaattcaa caaaatgtaa acaaatagaa aaccttctat attaagattt
      181 cctgcaataa caaacacaac agaagacgtg taatagttag tcaggaacgt acaaataacc
      241 cctgtatcct ttgcggtaca gggggttatt ttgtataagt agacattgta gtttctaaac
      301 tgctatgtcc taaacgtttc gatacgctaa gtatatttac tccttgataa agtaaaatag
      361 atgcatgcgt atgtcttagt ccatcaataa acagtcgaag agaaaatagg gaaaaattga
      421 ttactaaaga aaaacttact aggtcgacgg tgtagttgat tttcatactt caatagattc
      481 gaattaaacg atttcgattg gcttatacca gtcctatcca acgaaagata tatcgttctt
      541 aactctagcg gtataacttg aagctttacg taaagccaat gggaatgaac tggagatgac
      601 tttttattat ttttagtaat agctgatggg ttaatactta caactttcct tttcaaagta
      661 ccatataaaa aaacggtggg gttgtttagc gaaataaagg atctaagcca atagcttaga
      721 tcctttattc tatgctttat attatttttt gcatgtgtgc gaaagtatac tcaagcccaa
      781 tcgccatttc ggaaaatcgg tactgtagag ccatcttcac ggattccgtc gatatccatc
      841 ttgtcagatc ctaccatgaa atctacgtgg gtttgacttc tattcaatcc agcttctgct
      901 aattcttcat cggacatttc tgtaccgcct ttcacactga aggcataagc agaacctagt
      961 gctaagtgat tcgatgcgtt ttcatcaaat aatgtattga aaaagatgat gccagattga
     1021 gaaataggtg aagggtcagg tacaagtgcg acttctccta aacgacgcgc accttcatct
     1081 gtatcaagca gttttgctaa gacatcttct ccttgttcgg cagaaaaatc aacaactttt
     1141 ccatctttaa aagtaaattt catacccgag atgatggtgc cagcataact cagtggcttt
     1201 gtacttgaaa tgtaaccatc tactcgatga ctgtcaggtg ctgtgaacac ttcttccgtc
     1261 ggcatatttg ccatgaattt ttctccacga gcattatagc ttccagcgcc ttcccaaaga
     1321 tggtttttag gcaatccgat aatgatatct gtaccgggag cagtatagtg aagggcagaa
     1381 aattgttctc gattcaattc ttctgctttt tttgctaatt tttcatcatg cttcttccat
     1441 gctaagacag ggtcttcttc atagacacga gtcgttttga aaatctgatc ccataatgcc
     1501 tcaacttgtt tttcttctgg cagttctggg aagacttttg ctgcccattg tttaccagcc
     1561 gcagccacga cagtccaact gactttattt gcttgagttg ccttgcgtaa attcattagt
     1621 gctttgccat tcgctgattg ataagacgct actcgatggc tatctacacc agcaaatgca
     1681 tcgggatcgg cagaaacaac gctgatcctg cttgctcctt tttcaagcca ttcatccgct
     1741 tgatcgattt tagattgtgg tacattctca atacgatccg ttgcagcatg taaaagaaat
     1801 tctctttgga tctggtcgtc agtccattga acgataacct cagcggctcc tagtttataa
     1861 gcttcttgtg tgatcaatcg agcaaggggt gcctgttcca cgctgatctg taaaacaact
     1921 gtgtgtcctt tttctgtggc tacgccagtt tctgcaatta agcgtgcata tttttttaga
     1981 agttcattaa aatcagataa cataataaaa attcctcctc accatttttt ctggtaacta
     2041 ttagcatgat accatttaat tatgattcaa acaatgaggc tttaaataaa aaaaggaggt
     2101 tattttttta aggttaagtt tcatgatata tgttactact tgctatttca gcttgtgaag
     2161 aaaatcaaaa actaattttg catatcaaaa aaacatgtta caatgaaatt aatcaatggt
     2221 ggaagcgtta taaaaaaatt aaggagatta aaaaatatgg caagaaaaaa gacgattact
     2281 agagaacaga ttttggcagc tgcttatgaa gtagctgcca ccgatggatt tacacgattt
     2341 actgcgagaa acatcgcgaa caaaatgaat tgttccacgc aaccgattta tcttgagttt
     2401 aaaaatatgg atgacttgag acaagctctt tttgagcaga tctatgatca tttacaatat
     2461 gaagtgttca ctgttgagca tacagggaat acgattatcg atcttgcttt taattatatc
     2521 cattttgcaa gaagagagaa gaagttgtat cgttcactgt acttagaaga atatggtgga
     2581 ggaaaagaga tgcagaattt ctcttatact tactttgtga atacagtgaa acgcgatcca
     2641 gcttatgtga atctttcaga agagcagatc gaatctttgt ataatgggac atggatcata
     2701 gctactggga tcgcagcttt gatgtcttca agcatcatcc atccaactga tgaacagatt
     2761 gaaaagatga tccaagacag tatagatgct atcttgaaat tcaaagaacc aatcgaaatc
     2821 gattgaatat actcaaaagt tccacaaatc agtaggtaac aagagatgtg tcaaaataag
     2881 gaaaagttcg tttttcttac agcgtatgca tttttaactc ccgaagaact cttccatgaa
     2941 tgcaatgaat aaaagagcca tacagattga ataaatctgg atggctcttt tattgcaagt
     3001 atattaaatt tttgcgacta gttctttagc gaaagcttct aaattttcga tatcttcttc
     3061 ttcagcattc agatccactt taacattttc ggctcctttt gatgcgcctg tttttgcaaa
     3121 agcagcatcg aaatcatcca ccgatttgca gaattcatcg taaaaagtat caccagatcc
     3181 gcagacaccg taaatttttc ccaatagatc caattcttgc aagtcttcat aaaagtctac
     3241 gatctcatca ggcagatcgc catcatcgta tgtataagta gctactacgc aaatatctgc
     3301 ctcctcgaat tcatctgcat ctacttgggt acattcgttg atttcgactt cgatatccag
     3361 attttcaaat gcttctgcaa caatatcggc gatttcttca gtgtttcctg tcatgctagc
     3421 atatacgatt tttgctaaag ccatgatgtg cctcctcgtt tataattatt taacaattat
     3481 gaattatacc aaaacatcat tgaaaagaaa ataaaaccta aagacacctg gggaatgtca
     3541 tttggctaaa tgtatgccgc tatgttaaaa tgagagggat ttgtaaaaag gagacgaaaa
     3601 tatgattact ttaaaatcag cacgtgaaat tgaaatgatg gcagaatctg gagctttatt
     3661 agcagatgta cataaaaatc tgcgtgattt tataaaacca ggaatcacaa gttgggatat
     3721 tgaggtattt gtcagaaact atatcgagag tcatggcgga attgctgcgc aaattggctt
     3781 tgaagggtat gaatatgcga cttgtatcag tataaatgat gaaatttgtc atggattccc
     3841 tcgtaaaaag cctttgaaaa acggtgattt ggtaaaagtc gatatgtgta tcgatttgaa
     3901 aggcggcatt tctgattctt gttgggccta tgtagttgga gaatctacac ctgagatcga
     3961 ccgattgatg gaagtaacga agaaagcttt gtacatcggt attgagcaag cacaagtagg
     4021 gaatcggatt ggtgacatcg gtcatgctat ccaaacttat gtagaaggcg aaaatctagc
     4081 gatcgttcgt gattttattg gtcatggcgt tggtcctacg attcatgaag aaccagcagt
     4141 tcctcattac ggagaagctg gaaaagggtt gcggttgaaa gaaggcatgg ttatcacgat
     4201 cgaacctatg gttaataccg gaacttggaa aatgaaaatg gatcctaacg gctggactgc
     4261 gtatacaaga gacggcggac tttcttgcca atatgaacat acattggcta ttactaaaga
     4321 tggtccaaga attttgacat cacaaggaga agaaggcact tattgatcta tccagtcaat
     4381 aggtacggga gaggtacatg aaagtttgga acaaaataaa acaaaatgaa aatctgatgc
     4441 gctttattga gacgactcaa aagcgcatca ctgattcaga aatgagtacg acctctgtcg
     4501 tcgttgctta ttatttactc ttgtctttat ttccattgat tattgctttt ggtaatattt
     4561 taccgtttct ccacatcgat caagagactg tactgaccta tttacgagaa gtgattcctg
     4621 agaccgttta ccagtttatc ggtcctgcaa tcaaagacct tttgacacaa agttcaggag
     4681 gcttgttatc cgtttctgca ttagcggctt tatggtctgc aagtcaaagt atcaatgctt
     4741 tgcaaattgc gatgaataaa gcgtatggcg tagaaaatag aaaaaatttt ttgatcgtcc
     4801 gtttcttctc actaattgtc attgtcttgt tcatgattgc tattagtggg gtaactatcg
     4861 ttcttgggtt gggacagctg attttggatg ctattcagcc aatcgtacaa atacccgtga
     4921 actttatcga tcagttccaa actttgaagt ggccaatcac attggtagct ttatttgtca
     4981 tcatgttctt gatttattta attgtcccta atgctcagtt gaagttgaag acgatcattc
     5041 cgggcacgat cttcgctact gtcggctgga tgctgttatc acaagtgttt agtatctatg
     5101 ctaggtattt tgcaacgagg atcagcggtt atcagatcat aggaagtttt attgtactca
     5161 tgctttggct taattttgct gctacgatca tcattttggg cggaatcatc aatgcagttg
     5221 ttcaagaata cg
//
LOCUS       DAKBVG010000165         5200 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000165 DAKBVG010000000
VERSION     DAKBVG010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.165"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            176..952
                     /locus_tag="OUD09_002159"
     CDS             176..952
                     /locus_tag="OUD09_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HCT9693397.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            967..1170
                     /locus_tag="OUD09_002160"
     CDS             967..1170
                     /locus_tag="OUD09_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCT9693398.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            1186..1524
                     /locus_tag="OUD09_002161"
     CDS             1186..1524
                     /locus_tag="OUD09_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HCT9693399.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            1511..1690
                     /locus_tag="OUD09_002162"
     CDS             1511..1690
                     /locus_tag="OUD09_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693400.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(1733..2203)
                     /locus_tag="OUD09_002163"
     CDS             complement(1733..2203)
                     /locus_tag="OUD09_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693401.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            2290..2988
                     /locus_tag="OUD09_002164"
     CDS             2290..2988
                     /locus_tag="OUD09_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HCT9693402.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            3166..3507
                     /locus_tag="OUD09_002165"
     CDS             3166..3507
                     /locus_tag="OUD09_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693403.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            3500..4171
                     /locus_tag="OUD09_002166"
     CDS             3500..4171
                     /locus_tag="OUD09_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HCT9693404.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            4177..4863
                     /locus_tag="OUD09_002167"
     CDS             4177..4863
                     /locus_tag="OUD09_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693405.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            4866..>5200
                     /locus_tag="OUD09_002168"
     CDS             4866..>5200
                     /locus_tag="OUD09_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCT9693406.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVK"
BASE COUNT         1945 a          791 c         1033 g         1431 t
ORIGIN      
        1 aacaaaaaac actattgata tgtaaatgca gaccgccaag aaagctttaa aatcaatgtt
       61 tttatgtctt atttaattat gctcttagta tagagtatta ctcagatact gtcaattgaa
      121 tttagaaaaa agttgttaaa tttgtttacg aatataaaag aaaggaatga gaaatatgaa
      181 cacaccgcaa atttttaatt tcgaacaaaa cgaagttcgg acaattttag taaatgatga
      241 accatatttt gtaggcaaag acgttgcgag tgttttgggt tattcaaaca ctaaagatgc
      301 tttgtcgcgg catgtagatt tggaagataa gatggggtcg cgaatcacga cctcaggtca
      361 atcaagagag atgacaatca tcaacgaatc tggtttgtac agtttaatct taaaatcaaa
      421 acttccctct gccaaaaaat ttaaacgttg ggtaacaagt gaagtgttgc cagcaattag
      481 aaaacatgga ggttatctaa ctccagaaaa agtagaagaa gctttgctta atccagatac
      541 aatcattcaa ttagcaactc aactaaaaga agaaagaact ggaagattaa tcgcagaaca
      601 aaagattgca gagtacgaac ctaaaatctc ctatttagat agcatattat cttctacaga
      661 ttcagtaaca attagtcaga ttgcagcaga ttatgggatg tctccacaac agatgaataa
      721 attacttcat aaactaggta ttcagaaaaa agtcggtaac caatggttat tgtgcaaaaa
      781 acacatgaac caaggataca caaaatctca tacaactgag atcccgaaag ccgatggtgg
      841 cactaaaatt gtaatgaata ccaaatggac acagaaaggg cgtctattta tctacgaatt
      901 actaaaaaaa gaaggatatt accctcaaat ggatttagag gaaattggtt agaaaggagt
      961 tttagtatga ctgacattgc agaaatcact caacgagata gagaaaaaat caaagaatat
     1021 gtcgaaagtt caaagttctt aacttacacc atgcttgctg aaagatttgg aattagcaaa
     1081 agctacttat ctttaatttt aaacggtaaa aagacttctg cagaagcaaa cagaattata
     1141 gattcgatta tcactatgta cgaattgtaa agggaggaac agctaatgca atatctagaa
     1201 gcaaaaatcc caattccaga aggctatgta attatctccc aagtggatta tgaggagtta
     1261 aaaaaagctg atgatactgg tagatggatg acgttgccag aagtgctaga acggattaac
     1321 agaaaatatg attggtttac ttctagagtt ttaaagaacc caagatatag aaatattatc
     1381 gatatcgaga aaaacaaaaa tggatttgtc tattatccag ttgaaggaag agacacatat
     1441 ctatttttaa gaagtaaaac acttgaattt ttagaaacaa atttttcgga aatcttaagg
     1501 aggcaagcgg atgggaaaat ttaatagagc gctagtgttc agcgcaccgc taatcatcta
     1561 cgctttagga ctttggggaa gcaggcaagc gttgatagga acgattgttt acatggtttg
     1621 gatttttatg gggcttgatg aagctgaagc tgagtacaga gcgaaaaagc caaccgaggg
     1681 ggctgactaa aaatgaatag aaaagagaaa ctagaatgta tattactatt actcagttta
     1741 attctttcac taatttctct attgggaagt ttgtattttt gatatcaaca attttgtgga
     1801 tttgggttct atttagagta aatatcattt ttgtttggtg attaaggagt ttattaaaat
     1861 taattttccc tacttcaaat gcaaggtaac aagatatagc acttttagga ggaatattta
     1921 gcggtacagc ttcagagtat attctattat catcgactga tcctccttta gcaattaatt
     1981 tcttaaatgg agtagctgac catctgcggt ttaagttagc ggatgaaagt tctaaattaa
     2041 ctaacatagc tggttctgtt gaatagttag aaatgattac ttttgtataa atcatatctg
     2101 cagcgaagta agaagcatta agttcaactt ctagttgagg gcgcattcct tttattttta
     2161 tccccgttaa aaacgtactc aatataaaac ttattattga catccattga aaaattgtta
     2221 agtgaaaaaa attcaaaata aacaccacca gtttttaact aaattatacc aaaaaggaga
     2281 gaagaaataa tgcaagaatt agtaattttg aaaaataaag aagctgtgac tacgagctta
     2341 caagtggcag aaagcttcga gaaaaaacat caacatgttt taagagatat tgatgcacta
     2401 aaaaaagatg tgtccaattt tggacagatg tttgtagaag gtaatgaacc agattcatat
     2461 ggcagaaatc gacgagtttt cttcattagt agagatggat ttttcttgct ggctatgggg
     2521 tttacaggaa agaaagctat ctacttcaaa caaaaataca ttgaagcatt caacgaaatg
     2581 gaagatgtta ttcgcaagaa tactgttcct caaacaattg aagatatgat gatctatcaa
     2641 ttagaagaaa tgaaagatgt taaaaaagat gtttccatgc ttaaagatac tatgcgaatt
     2701 agcggacaac aagagtttga aattaagcaa aaaggaaata tgaaagttat gggagttcta
     2761 gggggaaaag aaagccgagc ttatgaagaa atcagcaaaa aagtattctc aaaattttgg
     2821 tctgaattta aacgtacctt ttcaatccca agatatggcg agttacctcg taagagattc
     2881 gatgatgctg tttcatttat tgaaatgtgg ttgccagaaa ctgcgatccg tatggaaatt
     2941 gatcaactga acagacaaca gagacttttt ggtgatgaca atgaatagag ctgaagcgct
     3001 aagaataggg acggcaattg ctaattgctg gtggaaatac tataaaccaa tcatcctaag
     3061 ccaacaacat attgacaagc aaaaagcatg gcaacaaata aaaaagtgac tccgccggca
     3121 agcaaagagt cacaaagaaa acacatcata aggagatttt agcatatgga aaaagaactt
     3181 tccactctag atcaatattt gattgatcct gattggggca agccgaaaat tgaggaaaca
     3241 agtggtcgaa aaatcagacg aaatcttttg acgaatgaag aactagcttg ggatcaagat
     3301 gatttaggca accatgtaac tatttgggat catgtttatc ttatccatct atcgaagcat
     3361 tcgaataaac ctgaatatat ttacgtcatc gaagatggct tgattgatgc gctagaagag
     3421 tacgacagag ataacttgat tgatatctct tattacggac caggtaagaa atacattgct
     3481 gaaatggagg cagaatttga tgagtgaaat caaagggaca acgaactttg aaaaactttt
     3541 tagtcgtaag ttaaataaaa ttctcaagaa aaaaggaaat tttgattatt tatcttgggc
     3601 tcacgcgtgg gagattatga aaaagaatga tccacaggca acggtaacta ttaatgagta
     3661 caaacactac agggttgttt ctggaactca tcaagacttt cttgttgagg aatataaacc
     3721 ttttcttatg gatgaaactg ggacttatgt atctgtctca gtaacggtta aaggacacac
     3781 ggaaaccgaa ttatttcctg ttttagatta tcgaaaccaa ccagttgtta aaccaaatgc
     3841 aatgcaaatc aataactcat tgaagcgatg ctttgtgaaa gcattggctc tacacggact
     3901 gggattatat gtatttcaag gggaagatat tccaacacca cctagaatcg atacaaagaa
     3961 attaaacatg ctagagacga ttctagaagc tttcaatgag cagatgggta aagatatgac
     4021 caaaacctta atcgaatatg ttaatgagca gacagataaa ttagggctct tagctgataa
     4081 cgttgaaact attgaacagt taagctatga gcaatgtgcc ttgatggagc gagcaatagc
     4141 agctaagaga aaagaattag ataagaagtg atatgagtgt ttaaaccatt aatcgattca
     4201 tattcagcgg ttctgaaaaa gttcaaagga aaagacataa gcgcaaccat caatgaggaa
     4261 gtgaacattg atcgactaaa gacgatgtat gacggctacg atggtgatcg agtcattgaa
     4321 attcgtttta ttgatcctag acgtttcacc gtacagcaac gaaacttcat ctatgcgctg
     4381 ataggcgata tttttatcga tacaggcatg ccaacggact tctggaagga attcttctac
     4441 tttcgttttg aaggtgtcac agggcgcaaa ataagcctga aagacgaatc gaatacaact
     4501 gtgagtgatg ccaatgtctt agcaaatatc atcttagatt tcatctttga acatcatatt
     4561 cctttcaaag aaggctatga gattttacct gcgaatcaag aatattactt ctacaaatgc
     4621 atcacaaaaa gagtctgctg catctgtggc aaaacaggag ctgacatcga tcactttgac
     4681 aaagcgctag gaagacgaaa gcgcaaagaa gttgatcatg cagagtacac atttgcagca
     4741 ctctgcagaa tccatcacac agagaagcac aaaataggtg tgattaattt caaaaataaa
     4801 tatcaaatca aagggatcaa attaaaccag aaaacaatca aaaagttaaa tattggaggg
     4861 taaaaatgac agaacatcga agttattatg cgattatacc agccaacgta aggtacgaca
     4921 aaagacttaa accaaatact aagttgttat acggagagat aacggccttg tgtaatgaaa
     4981 gaggcttttg ttgggcaggc aatgagtact ttgcagattt atatggtgtg aataaagaga
     5041 ccatatcgcg atgggtaagt gatttgatta agtttggata cttgaatcgg gaaatcattt
     5101 acaaagaggg taccaatcaa ataatcaata ggtacctacg aattaatcaa taccctattg
     5161 acgaaaaacg caatacccct attgacgaaa aagtcaaaga
//
LOCUS       DAKBVG010000166         5182 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000166 DAKBVG010000000
VERSION     DAKBVG010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5182
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.166"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(422..919)
                     /locus_tag="OUD09_002169"
     CDS             complement(422..919)
                     /locus_tag="OUD09_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCT9693407.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(959..1768)
                     /locus_tag="OUD09_002170"
     CDS             complement(959..1768)
                     /locus_tag="OUD09_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCT9693408.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            2102..2959
                     /locus_tag="OUD09_002171"
     CDS             2102..2959
                     /locus_tag="OUD09_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HCT9693409.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            2952..3854
                     /locus_tag="OUD09_002172"
     CDS             2952..3854
                     /locus_tag="OUD09_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HCT9693410.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            3887..4237
                     /locus_tag="OUD09_002173"
     CDS             3887..4237
                     /locus_tag="OUD09_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HCT9693411.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            4286..>5182
                     /locus_tag="OUD09_002174"
     CDS             4286..>5182
                     /locus_tag="OUD09_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HCT9693412.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYH"
BASE COUNT         1745 a          887 c          974 g         1576 t
ORIGIN      
        1 aaccgtccgg tcgtaggttc gagtcctaca aggtacataa gaggttgtga aaaagctgcc
       61 ctgcatcaag taataagaac agccaagtaa aaacagcttc ttatgttttt acttggctgt
      121 tcttattact gcagatgcaa gcttttgaat accgtttaag gaagaggttg tgaaatcagc
      181 gttttgacct gagtattagc ttaacaaacg gaaaaatagt tcctcatatt ttgccgtttg
      241 ttaagctaat accgagggtc agctgattga acaccattta agaaagaggt cgtgagcctg
      301 ccgtttcact ctgtgtcata aaaaagccaa tctactttga tagaaaaagc acgtaggact
      361 aaaacagttc tgcgtgcttt ttttattgtg tgttaaatat cgtaaatgaa gatagatatt
      421 tttatgattc gacgatagag acgttgaaat ctttcaacag ctctaaagcg gattctccgt
      481 gttttctact ggagactaac attggatcaa caaaaaagtc tgcttcagga aaagcactct
      541 gcaggcctat cagattagag agtacacaca agtttgtttc aatcccgact acttcgatct
      601 ctttttgttc ttctttgctt tcaaataaag tttcctggat ttcagctaaa gtttcaggtg
      661 gaatcgtaaa gtaatacttt ttaatcactc gttcattttc ttgtggagaa agaagaggga
      721 tcaattgcaa atcttctcgt tcttcttctt tgtccttcag ttcaactggt atatcccgtg
      781 tgaagagaat atattcatta ttttggcgtg ctttagctaa tctttgagca atacgttctg
      841 ccaattgatc tgactctgga atataaaatt cgctgtttgg atcaagaata tgattttgca
      901 tatcaataat aactaacatt ttctttcctc ctacattcat atcgtcattt tattcttttt
      961 aatagaagtt ttttaaaata taagcaacac cagcttcttc attcgtcagt gttacttggt
     1021 ttgcaatttc ttttacttgt cgctgtgcat tccccatcgc aatactcaac ccgctgtaat
     1081 tcaacatagc aatgtcgttt tcttgatcgc caaaagccat gatgtcactc gattcgattc
     1141 caaggtaatc cgccagaaaa acaagtgaat gagctttagt gatacctgta ggcgtgattt
     1201 ctaagaaatg cggatcagag aatactgatt ggataccatc tgcttgcaga tctctttgta
     1261 gcttctgtag tttttcttca ctcttatctg ttaatgctaa tttaagatag ctaagagaag
     1321 gatcttccaa catatttaaa atttgatcaa aagaatggtt ctgtaaattg aaatgttcat
     1381 aataagggtc atctttgact agatttgatt catcgaatgt cagctgatca tcttgggttt
     1441 ctcggctagc aatgatggat attttcgctt cttgagcata gaagaaggct tttctcagtg
     1501 tatcgctatc tatttttgaa cgtctgagaa ctttcgccat ttttccatcc ttcatttcag
     1561 caatcagtga accattggaa gaaatcaaat agccaccgat tcctaattcg gaaaaaatat
     1621 ctttagcaga aatgctgttc cttccggtag cgacggttac gattccgcca ttttcatcaa
     1681 atttccgaag tgcttggata ttttcttctg gtatctttcc atttcgatca agcaaggtac
     1741 cgtctaaatc aatcgctgct agtttcattt tatcttccta ctttcttttt acatatctat
     1801 tttcctgtta ccttaagatt aaatgagaat ttaaaaaaat gaaactaact tgctttgtca
     1861 ttatgaattt tttcaaaaaa cattcttaag aaaaaggtgg ctatatatag tagtttttgg
     1921 tcaatacctt tgaataaaaa gccatgaaaa acgaatagtt agcgcttacc gatgcgtttt
     1981 gtgataaaaa aattttaaaa tattcaatga aaacaaagca tatattttgc tttaaaacca
     2041 aatgaggaat attctagatg tagaacaggt tagaaaaaga ttagaaaaag gagtgatttt
     2101 gatggagaag agatacggcg tagttcctgt ggtaactgct tcagacgaaa attatgctcc
     2161 ttacttgagc gtgatgatcg caactgcatt agaaaattgc aataaaacaa gacgcataaa
     2221 attttatgtg attgacgatg gtctatcaga atatagtaaa gaaggattgg aagaaacggt
     2281 aaataaatat tcaagtaatg caagtatcca attcctgaca gtggaaaaag atatctacga
     2341 agacttccta gtcagtgacc atatcacaac taccgcttac ttaagaattt ctttaccaaa
     2401 cttattagct aaagaggatt ataagaaagt cctttactta gattcagatg tgttagtact
     2461 agacgatatc gtaaaactat atgatgagcc gttaaatgga aaaacaatcg gcgctattat
     2521 cgatccaggc caagtgaaag ctttggaaag attaggaatc gattccgatg acttgtactt
     2581 caattcagga gtgatggtga ttgacattga tcaatggaat aaaaaagaaa ttacagaaaa
     2641 aactatccat tacctcagtg aaaatggcga tcgaatcatc tatcacgacc aagatgcttt
     2701 gaatgctgta ctttatgaag attgggaaca acttcatcca aaatggaaca tgcagacttc
     2761 gctgattttt gaacgtcatc cagcaccaaa tgaaaaatat gaacgtcagt ataaagaagg
     2821 aaatgaaaaa ccttcgatcg ttcactttac aggacatgat aaaccttgga atacgttgaa
     2881 agatcatccg tataccaatt tatacttaaa aaaattagca cacagtactc taacgaaagt
     2941 aggcgagatc aatgaataaa aaagaaatag cagtagtcgc aagttgtaac acaaaattcg
     3001 taccacattt agctgcatta tttgtctcag tattagataa ttgcaacccg tctaaatttg
     3061 ttcggttcta tgtgattgac gatgatatcg attttgaaag taaacagctt ttacgcttct
     3121 cagtgaaaaa cgcacgaatg aactcagatg tggaattctt gaaaatcaat aaagaattct
     3181 ttacaaatgt ggtaatcagc gatcgtattc cagaaacagc ttattatcgt attgcgattc
     3241 cggaattgtt ccgcggcaca gaagttgaac gtatcttata tatggattgc gacatgatcg
     3301 ctttacaaga tatctctaaa ttatggcgtc ttgacttcgg tgattcaatc gtagcagctg
     3361 ttgaagacgc tgggttccat caacgactgg aaaaaatgaa aatttcagct aaatcaatgc
     3421 gttatttcaa ctcaggtttg atgctgatca atgtgaaaaa atggttagat gaaaatatta
     3481 cgcaaaaagt attagatttc atcgaacata atccagaaaa attacgattc catgaccaag
     3541 atgcattaaa tgctatctta catgaccgtt ggttaccact tcatccgcgt tggaatgcac
     3601 aaggctatat catggcaaaa gcaaaaaaac atccaacagc agcaggtgaa cgtgaatacg
     3661 aagaaactcg caacaatcct tacattatcc atttttcagg acatgtgaaa ccgtggagca
     3721 aggattttga agggccaacg aagaaatatt acgaaaaata tgcagggatg acggctttcc
     3781 gttgtgtagc taaattccct aaatatccaa aatatgcaaa agttcaacca aaaacagatg
     3841 tagttgttaa ataatagaag acaacaactg aagaggggga gtaggaatgg aaattacaat
     3901 gtctaaaaat gcagtagaga cgttaattga aaaggttggg gaaaatacaa aaatcgcact
     3961 cgcgcttatc aatgattccg atccatttct aagggacaaa ggtgcttttg caaaaggcag
     4021 cttttttcaa atcattcctt ttgtttcgga atttggagaa tatgcgacta aaatcgagca
     4081 tccattattg gacatctata catctaaatt agagcaaaat tatttcggta aacgattgaa
     4141 catggacttc aacaaacagc ttgacagctt ttcattagaa aatgaaatcg cagtactcga
     4201 ttataatatc aaattaaaga actgcttctt cagctaatag tttcgagaaa gtccaaaaag
     4261 gaggtataca ttgagatact actttgtgga tgaagaaaag aatgtaatag aaacagatga
     4321 aaaaaaatat aattggctcg tacttgatag tgcaaagccc gaagagattg agcaagcaat
     4381 cgaagaattc gagcttcctg atgatatttt cgtaggaacc gaatatccgg aagaagtttc
     4441 tcggatggag cgcctttatg agacgaaact agagcatcca ttttctttgg tagtgattaa
     4501 tctagatcag tcagataatc gtatcgaaaa aaaattaacg ccaatatctt ttgtactttc
     4561 cgataatctc ttgattactt gtatcgatag aaaaaccgaa tttgtggatc ggctgctgac
     4621 aaagcatgga aatcatttaa actcgtttga aaagatcatc acgtatacat tattagatat
     4681 ttacacccat tacgtgaaag aactaagaga gatgaaaaag cgaattgacg ctttggataa
     4741 agcagctcgt aaaacaacgg aaaatgaaga actgtataaa caggcagatc ttgaaagaga
     4801 catcgtatat atcgatcata ccttaagaga tcaacgggaa acaatggatc aattatggaa
     4861 ttctgaaact ttcatcaaac gtttagacga tgaacagcta ctctatgatg tccatttgag
     4921 acagcgacaa acagaaaaaa tgatcgagat ctatcgagat ttattagaga gtattggtga
     4981 tctattcaac gggatgatgg acaataatct aaatcatatc atgaaatatc tagattcagc
     5041 tgcattaatc atttccgttc cggcgttgtt cgcagggatt tggggaatga atacaggagg
     5101 attaccgggt aaaagttctg ctctaggctt tatactcgta gtctttggct ctttgatagc
     5161 agcagtagtt ttaggttacc at
//
LOCUS       DAKBVG010000167         5153 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000167 DAKBVG010000000
VERSION     DAKBVG010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5153
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.167"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(52..633)
                     /locus_tag="OUD09_002175"
     CDS             complement(52..633)
                     /locus_tag="OUD09_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HCT9693413.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(639..1898)
                     /locus_tag="OUD09_002176"
     CDS             complement(639..1898)
                     /locus_tag="OUD09_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCT9693414.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIAPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(1900..2946)
                     /locus_tag="OUD09_002177"
     CDS             complement(1900..2946)
                     /locus_tag="OUD09_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693415.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQRPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(3181..3456)
                     /locus_tag="OUD09_002178"
     CDS             complement(3181..3456)
                     /locus_tag="OUD09_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HCT9693416.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(3699..5009)
                     /gene="der"
                     /locus_tag="OUD09_002179"
     CDS             complement(3699..5009)
                     /gene="der"
                     /locus_tag="OUD09_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCT9693417.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
BASE COUNT         1465 a         1085 c          847 g         1756 t
ORIGIN      
        1 gattccacat ccagctgatc tttgtctttt ttagtactat actattttta actaattgtt
       61 atgttcactt acccggttac ttttcttttg tgtctgcaaa aggatctttc ggttaacttc
      121 atcagataat ttcaaaaaag catccctatc tccatcttct agtgcttgat cgatttgagc
      181 ataaagcatt tttagttttg cctcttcttc tttttcttga aaatatcgtt caattaattc
      241 agtatcttct tcactgactg aagaatccca agaagaaaat ggattgtctt ctaagataga
      301 tagatattct cttgtctgcc aagcattttc gaatatacat tccacatata aaggcttttt
      361 ccaattcaaa cggatttcat gaaaaatctg gtctgtgtcc gtaaattctt ttccctgcaa
      421 aaacaatttc atgggttccc cttcaacaga tagatcactg atctgcagtc ctcgatccgc
      481 aaaattcgcc ccttcaacaa agtggacatt gcttaaaatc gcttcatgat tggcaagata
      541 attcaagatc caaaaaactt ctcttcggct aaaagataca tgattgacca tccacgttaa
      601 aaaattcttc ttttcccgta catcaattag catttttctc acctctctgt taggtcatct
      661 aataatgaca gtgcctccat atctcctggt tcaagtgtta aataataagt caataattct
      721 tgcgctcttt tcagctgccc ttcttctctt aagaaaaagg catactcttt caagaaatct
      781 ggttcatgtg atagttcatg ataagcctgt tcatagtgta cagctgcaac ggtaaagtct
      841 tctaattcat tataagcatg ggctaggttc cattctgcat aaggattagc agtttcttcc
      901 atctgattga tcacttgtat cacatcttca tatctttcat catccaagta aagattgctt
      961 aaagtaagta atgtttcatc ttgtttgtct cctacttcta aagctttcag taaaagttct
     1021 tctgctcgct gtctatcatg cagacggaaa gcattttccg aggccaggtg ataaagttcg
     1081 acttggtaag gattttcttt gattccttct tctaacactg tttgtgcttc ttcaatcatt
     1141 tcttcttctt gaagagcttg tcctagatat agatataacg attgataatg cggattaact
     1201 tctcgaagtt cttccagata gttgattgcc tgctgatttt ctttcaattg aagattcgta
     1261 aaagctagct ggaacagtcg atcatctgtc ctttcttctt tcagtgcttc ttctaaaggt
     1321 gcgatagctg cttcgaattc tccttgcata cttaaggatt gtcctaaacg ttcctgcatc
     1381 gagattcctg aaatttcatt tatcccgctt gccaacaatg tttcataaac cactgctgcc
     1441 tctttgaaac gatcaactga aaaatacaat tcagctagcg caaactggat caatggttca
     1501 tcaggaagca acccagctgc ttctttcaat tttgcttcac tcacttcagg gattccgata
     1561 acttggtaca aatcagctat cgccaataag ctttgcggat agtaatcact ttgcttatcc
     1621 actttttcca ggtattcaaa agctaagtcg atctcattat cttcgattgc aatttctgct
     1681 aacgggatgt tcagtccgtc attttctggg tattggttga tcagttgttc aaaaatctgt
     1741 tttgcttcga tcaaaaagcc aatagccagc aattcttcgc ctagttcagc taatatatct
     1801 gctgggtctt cttttaatgc tttttctaaa aagatttgtg cttgtgacaa gtcttcatct
     1861 gataaagctt gcaacatttt ttcgctattt gtattcattt caattccctc ttatctcact
     1921 gacgatttgt tgaaataccg tcattgttct caataattct ttttcgtttg gacgttggta
     1981 tccgtctatt atacctattt tttctaaaac aagtattttt ttgctttttt ctgccaaaaa
     2041 aagaactttt tccaaatact ccatctttga aatctgttcc agccagtgga ttggatagcc
     2101 aagcaagtcc agccacacaa aaaagttttt tagttggaaa tcaaaaggat atgcttggag
     2161 attccacacg aaatgaaaca tcattcctaa aaattttttc atactagcag aaagtagatg
     2221 gccattttcg gtcagataaa aagcttgttc aaacagttta ccataatttg acaggttttc
     2281 tgcctctaat tgataaaaat agatcatttc ttcgatcatc ccggcaaacg gccgagtcgt
     2341 taattgcttt ttatttggat agtttttata cagattttgc aaaaaacgat gatcacaaat
     2401 cattccgcaa cagataaaag tcaaaaagtc aatctgcttt ccgtccactt gccgctcgct
     2461 gattgtttga tcataaacga tgcgttctgg cagattgatg gtttgcagta ctacacgatt
     2521 tggtagctgc aaaatcgtcc gctgcggtgt taatgctcgg cttagagaac ggatcgatac
     2581 agggatcatc catagtgatg aatcaagggt tgtatgtttt tgaaaaaaac ctgccagatc
     2641 catgatccct tcatttccaa atccgatgat aaacatggat tgattttcgg gatagcgttt
     2701 gacgaaatca agcaggttct ttaattctgt taaatgattg cattgagtgt tggcacaaat
     2761 ataccagtca ttgtttgctt gatttgcaaa taacgggttg atcttatctg caaacaaatc
     2821 ataatagcgc tgattcgtta aaaataagat tggctgatca atggaaagtt ctcttagctg
     2881 gctgactagc gtttctccgt aaaacacttt cgtcaacgca tcttctactt gataatcaag
     2941 ttccatacgc atccctcctc ttcttatttt tattttacca ttagtaagca aattctagaa
     3001 aagataagag caaagctcac ctcataactt ggaaataaat aaaaattaaa taaaatacat
     3061 gtgttttcta tgtatgacat acggattcgg aatctctgtc tcttatttat tccttttctg
     3121 ctaggaaata aaaagaaaag cacttgtagc cctagagcca caagcgcttt ttcaactgtc
     3181 ttatttaaca gcgtctttta atgctttacc tggtttgaat gcaggtactt tgctagctgg
     3241 aatttcgatt tcttcaccag tttgtgggtt gcgacctttg cgagctgcgc gttcgcgaac
     3301 ttcaaagtta ccaaaaccga ttaattgaac tttttcgccg ttagctaaag catcttgaat
     3361 agttgaaaat acagcatcta ctgctgcagt tgcatctttt ttagttaaat cagtagctga
     3421 agcaactttt tcgattaatt ctgctttgtt tgccatggat agattcacct cctccacaag
     3481 agatatgata gaaatatcat ttagttaaaa ctttctgagt ggtccagaag tttaaaaaga
     3541 aatattgatt aacttcaata ttctgttatc acgatatcat atgaaccgct taatagcaag
     3601 gtttttacca attttcacat gcttttttta gtcatagaaa cgtaacaaac gaaactgtct
     3661 ttttcttttg aaaaaaaaac gtcgatatgg taaaatgcct attttctcct acgagggata
     3721 attcggatgg acgtcccttc aaaagtaaat gcttttctga tttgattttc taagaaacgt
     3781 tcataagaaa aatgcatcaa ttcctcttca ttgacaaaaa cgacaaatgt aggtggtttg
     3841 acagcgactt gtgtagcata aaaaatcttc aggcgttttc ctttatctgt tggtgttgga
     3901 ttgattgcta cagcatccat aatgatatca ttcaacacag ccgaagagat acgaagattc
     3961 tggttcatac ttacttgttc gatcatttca ggcagacgtt ccaaccgttg tttagtcact
     4021 gcagaaacaa aaacgattgg agcgtaatca agataccgga attcatcacg aatctcttgt
     4081 tcgaaatcac gcattgtatt ggtttctttc tttacaagat cccatttatt cacgacgata
     4141 ataattccac gaccagcttc atgggcgtaa cccgctactc gcttgtcttg ttcccgaatc
     4201 ccttcttcag cattcagtac catcaaaacg atatcagaac ggtcaatggc acgcattgca
     4261 cgcatcacgc tgtatttttc agtattttcg tatacttttc cgcgtttacg cataccagct
     4321 gtatcgatca ttaagaattt ctgaccactt tcagaaacga agtgggtgtc gattgcgtct
     4381 cgggtcgttc cttcaatatc cgaaacaatt acacgttctt ctcctaaaat cgcattgatc
     4441 aaagaggatt taccaacatt tgggcgacca atcaaactaa acttaatcgt gtcgtcgtcc
     4501 tcttcttcag tctcttcaga gaaatacttc actgcttcat ctaaaatatc accaatccct
     4561 agtccatggc ttcctgaaac aggataagga tcgcctagcc ctaaggaata aaattcatag
     4621 atatcgtttc tcatttctgg attatctact ttattgactg ctaaaatcac tggtttatta
     4681 cttctgtata aaattttcgc aaccatctca tctgcgtctg tcacgccttc gcgtccgctg
     4741 actacacaaa tgattacatc tgcttcttca atcgcgattt ctgcttgatg cttgatctga
     4801 tccataaagg gttcatcgcc aagatcaatt ccgcctgtgt cgatcacgct gaattctcga
     4861 cctaaccatt caccttttgc ataaatacga tcacgagtta ctccaggagt atcttcgaca
     4921 atcgagatac gctcaccagc gatgcggtta aaaatcgtag acttaccgac gttcgggcgg
     4981 ccaacgattg caattgttgg atttgccata ttatttcctc ctcacaagtt tccaatctca
     5041 cttagtttaa cgaatgcatt gccaactttc aagcatagat tgtcaattaa acgaagtatt
     5101 tttattcctt attgaaatac taatcgcttt tcaaaagaaa aactgagaca aaa
//
LOCUS       DAKBVG010000168         5133 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000168 DAKBVG010000000
VERSION     DAKBVG010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.168"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            150..1865
                     /locus_tag="OUD09_002180"
     CDS             150..1865
                     /locus_tag="OUD09_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT9693418.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            1868..3631
                     /locus_tag="OUD09_002181"
     CDS             1868..3631
                     /locus_tag="OUD09_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT9693419.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(3671..4207)
                     /locus_tag="OUD09_002182"
     CDS             complement(3671..4207)
                     /locus_tag="OUD09_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HCT9693420.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(4530..4952)
                     /gene="mgsA"
                     /locus_tag="OUD09_002183"
     CDS             complement(4530..4952)
                     /gene="mgsA"
                     /locus_tag="OUD09_002183"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCT9693421.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
BASE COUNT         1546 a         1027 c          986 g         1574 t
ORIGIN      
        1 aaatagtcta ttcaatttct aaagcttctt cataataaaa ctttcattta tgaaaaggct
       61 tgttctcttt tcattctaaa tttcctaagc tacaatgtgg tagtacttgt tttttcaatt
      121 gtggacaaat aaataatagg aggaatcata tgattcagtt attacgttat gcaaaagact
      181 atcgtaagca aatcattttg ggcccgttct tcaaatttct tgaagctgtc ttcgaacttg
      241 tcctgccttt aatgatggct tccttgatcg ataatggact aaaaatgaat gaccgaggaa
      301 aaatcattga aatgggctta tggatggtcg ctatgtctgt gattggtctg atttgtgcta
      361 tcatttgcca atactatgct tcagtagctt cacaaggatt tggtacagag ttacgtaatc
      421 agctaatcaa aaaaatcaat actttctctc acaacgagtt gaatcatttt ggaacagata
      481 ccttgatcac ccgaatgaca aacgacatca atcagcttca gctagcttta gcaatggtca
      541 ttcgtttatt gatcagagca ccgtttttaa gtatcgggtc agtggtcatg gcttttgtga
      601 tcgactggga agtaggtctg tttttcttag cattattgcc tattttctca atcattttat
      661 tttttatcat caaaaaaacc gttcctctct accaaaaagt gcaagaaaaa ctagatcagc
      721 tcaatgaaca cgttagtcag aatcttagcg gtgttcgtgt catccgcgca tttgcgcaga
      781 caaaaaaaga aacagagaag ttcgataaaa gcacaaatga tctagcgaac aattatttac
      841 gtgtttccaa tatctctgcc ctattatcac ctgctacaac cttgatcatg aatgtcggga
      901 tcatctgttt gctgacagtt ggcgggatca aagtaaatat tggtagcttg caacaaggac
      961 aagttcttgc tttgatcaat tatatgaatc aaatgctttt ggcactgatc gttgtttcaa
     1021 acctcgtcgt gatcttcaca cgtgccgaag cttcaggaaa tcgggtgaaa gaggtgctag
     1081 acacagaaaa ttcgattctt gaagctgagg tcagtactac tcccgatttt tcttctgaag
     1141 caattatcgc ttttgaccat gttgacttcc gttatactcc tgattctggt ctttctctcc
     1201 aaaatattac atttcagctg aagcgaaata cagtccttgg aatcattggt ccaaccggaa
     1261 gcgggaaaac gactttgaca caattgattc cgcgtttcta tgatgtcagt tccggtacag
     1321 ttataatgga cggaatggat gttcgttctt ggcagctgga tcatctccgt cagcaaattg
     1381 ctcttgttcc ccaaacttct gtactattta ccgggactat tcgtgaaaac cttcaatggg
     1441 gtaagaaaga cgccacagaa aaagaatgtt gggaagctct gaaaatcgct caggcagaag
     1501 aatttgtccg tcagcttcca aatggtttag atacaaaagt catggaaaat gggaaaaatt
     1561 tctctggcgg acaaaaacag cgactgacga ttgcaagagc tttgattgcc aagccagcat
     1621 tactgatttt agatgactct ttaagtgcgt tggattatca aacagacttg gatttgcgta
     1681 aggcgctaca gacttatctt ggcagtacag tgattattat ctcccaacgt gtccgctcta
     1741 ttcaagaagc acatcatatc ttagtgatgg atcaaggtaa gattgctgct cagggtaccc
     1801 atgaagaatt acttgctcaa tcagcagaat atcaggaaat cgtcgcatca caggaggaag
     1861 aatagttatg aaagtaaaaa aattatcctt gcagacgctc cgccgattca ttccttacct
     1921 tactgcctat gcaaaagaca tttggctagc tgtgatacta ggaatcatca atgggattgc
     1981 aacagttgtg atgacgttac aaattggtca aagtatcgat caaatgattg gagaaggaca
     2041 ggtccgcttc aatcgactgt tccatttact tattctattc ggtggtatcg ttttaatcaa
     2101 cgtagtcagt caatggctca tccaagtgtt aagcaatcgg ttagcttact tatccgttgc
     2161 aaaattaaga aaagatgcat ttgctcatct gaatcgattg ccacttcgtt attatgatca
     2221 aaactcccac ggaaatatcg tcagtcgatt tacaaatgat atggacaata tttccattgc
     2281 aatttctgcg gccttcaatc aattattttc tggagtggca atcatcattc tttcgtttat
     2341 ttttatgatg cgtctaagtc cacttctgac attggtcgtt ctttgttcta caccaattat
     2401 tttccttgtg agttggatcg ttgctcgtgc ttcacaaaca aatttcgata gacagcaaag
     2461 gattattggg gatatctcgg gctttgtgac cgaacgaatc ggtaaccaga aaatcgtgaa
     2521 agcttttcag caagaagaag ctagtcaaca gcaatttgaa ctgctaaatg ctgatctata
     2581 tgaaaaagga caacgggcac aattttcttc ttccttgaca aacccgtctt ctcgttttgt
     2641 agatcacctt gcttatttat cgattggttt agttggcggc ctattggcat tgaagagtgg
     2701 ttcgtcaatc actgttggtg tgatttccag ctttacgatc tactcaagtc agttttctaa
     2761 accatttatc gaactttctg gaatcacgac acaactgcaa accgcttttt ctggtctaga
     2821 acgagcattt gatttgatcg atcagccaga agaaaagcct gatttgccag atgcctatgc
     2881 cttggaaccc aaaaatatca aaggaaaagt ctcttttgat catgtttctt tttcttatga
     2941 ccccaagcag cgtttgatcg aagatttcaa ttttactgct tatccaggtc aaaccattgc
     3001 tatagtagga aaaacagggg ccggaaaatc cacacttgtt aatttgctga tgcgttttta
     3061 tgacacaaca aaaggaacaa ttacagtcga tgaccatgac atccgctcta tccaacgtga
     3121 tacattacgt aaaagttttg gaatggtgtt acaagatact tggctatttg acagtacatt
     3181 gcgagaaaat cttcgttttg ggaatccgga agcaagtgac gcagaaatca atgatgccct
     3241 aaaagcctct tatatgtatg aatttgtaga acgtttgccg gaaaaattag atactaagat
     3301 tggcggtcaa gggttaaaga tatcagatgg acaacgccaa ctgttgacga ttgctcgaac
     3361 gatgatcagt aatcccccta tgttgatctt agatgaagct accagctcag tcgatacatt
     3421 gacagagaaa aaaatccaac aagccttctt agcaatgatg gaaggaaaaa ccagttttgt
     3481 gattgcccat cgcctgtcca caatcaaaaa tgcggaccag atattagtga tggatcaggg
     3541 agctattgtc gaaatgggca cccatgatga gctgcttcaa aaagatggtt attaccgacg
     3601 gctctatcaa gcacaattca cagcacaata aaaacaagca gtcttctggg atgggaagac
     3661 tgcttgtttt ttatttagcg tggatcgcta aacttcttaa aatttttttg taaggaatac
     3721 tacgtcctac gtaataaatg gcaattgatt gaatcggtaa ccatattact gtttttaaca
     3781 ggcggggcgc ccaaacgacc cagctgaaca atggcacatg gtacatcaac gcaagccata
     3841 atggtgtcaa aaataagttg atgatcagcg tgacactaag cgtagccaga atggcatttt
     3901 tccaagtgat ctcttttcga tagaaaaaga atccgtagat tgctccttca ataaatgcat
     3961 tcaatgtaaa tccaataaag aatgcagatt tcgagaagag ggccattcct actaaatcag
     4021 ctaaaactgc gcctatccct gaccaaaaag gaccaaaaag aatgcccatg atcacttgtg
     4081 gaataaatgt gacactgatt ttaataaatg gggtctcgat tgctaccagt cgggacaaag
     4141 tgaccatcaa tgcaatcaat aagcccataa tagcaatcat acgtgcgtct aaccggtgtt
     4201 tcttcatttt tagcatctcc tttaagagct gctacatcgt atcatgcagc gaatgcgaaa
     4261 ataaaaccgc gaacattacg tccttcgtcc aagaggcaac atcccatcct cttagcactt
     4321 aacgctttat tttctactct gtattatttt tgggatcttt tcccacagaa actatagcat
     4381 attttcacat ttactgcaaa tacgaaaaac taatattatt gtttatatta tccaatacgt
     4441 tcgtattata tcgcatatta tcagtttgtt tagcttttat agctacatat aggcgaacat
     4501 taaacgttat ttttccatac gcttatccat caaaatgaaa gtggatgctt gtctatttca
     4561 tggacgattc ttcgaaaatc taggaagccg gcttccagtc ctcttagtag cacctcagct
     4621 gttccgatat tcgtagctaa cggaatctca tagacatcag ataaacggat aagtgctgtt
     4681 acatctggtt catggggctg tgcagccagc gggtctcgaa gaaaaatcac caaatctaac
     4741 tgatcctccg aaatcatggc tccaatttgc tgatccccgc ctaacggtcc ggatttaaac
     4801 cgatggatcg gcaagccggt tgcttccgtg atcctctgcc cagtcgtccc ggtagcaaaa
     4861 agttcatgtt tttccaatat tgttttatac gctgttgcta atttcaccat cataggtttt
     4921 tttcggtcat gagcgatcaa agcgattttc ataagagaca ctccttttga tattgttaac
     4981 atacttattt actccagtgt atcagttttt atttcttatg gatatttatt aaagcttaaa
     5041 actagcacat tgacaaaaaa aagaaaaaat tcttgttcaa catgacattg aagttagtta
     5101 acgctttcat ttatacttta aatagttaat aaa
//
LOCUS       DAKBVG010000169         5052 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000169 DAKBVG010000000
VERSION     DAKBVG010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5052
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.169"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(<1..182)
                     /locus_tag="OUD09_002184"
     CDS             complement(<1..182)
                     /locus_tag="OUD09_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCT9693422.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="OUD09_002185"
     CDS             517..951
                     /locus_tag="OUD09_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCT9693423.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="OUD09_002186"
     CDS             974..1294
                     /locus_tag="OUD09_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693424.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="OUD09_002187"
     CDS             1435..1626
                     /locus_tag="OUD09_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693425.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="OUD09_002188"
     CDS             complement(1697..1954)
                     /locus_tag="OUD09_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HCT9693426.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="OUD09_002189"
     CDS             2112..2375
                     /locus_tag="OUD09_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693427.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="OUD09_002190"
     CDS             2399..2587
                     /locus_tag="OUD09_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693428.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="OUD09_002191"
     CDS             complement(2670..2855)
                     /locus_tag="OUD09_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693429.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="OUD09_002192"
     CDS             3070..3441
                     /locus_tag="OUD09_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCT9693430.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3591..3663)
                     /locus_tag="OUD09_002193"
     tRNA            complement(3591..3663)
                     /locus_tag="OUD09_002193"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3628..3630),aa:Gln,seq:ctg)
     gene            complement(3709..4137)
                     /locus_tag="OUD09_002194"
     CDS             complement(3709..4137)
                     /locus_tag="OUD09_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693431.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4197..4955)
                     /locus_tag="OUD09_002195"
     CDS             complement(4197..4955)
                     /locus_tag="OUD09_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HCT9693432.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
BASE COUNT         1762 a          784 c          987 g         1519 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca
     3541 aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc
     3601 agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc
     3661 ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg
     3721 atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc
     3781 aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac
     3841 aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata
     3901 ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc
     3961 gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct
     4021 gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg
     4081 gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat
     4141 ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag
     4201 cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg
     4261 atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca
     4321 tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg
     4381 gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag
     4441 acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga
     4501 aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag
     4561 acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca
     4621 tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta
     4681 gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt
     4741 tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc
     4801 tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct
     4861 ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct
     4921 agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctcccttt tcagctgttt
     4981 atctttccta ttataaatgt atttttattg gaaatggaag tattatattt taatatgtag
     5041 gaaataatta gt
//
LOCUS       DAKBVG010000170         4980 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000170 DAKBVG010000000
VERSION     DAKBVG010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.170"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            1..1114
                     /locus_tag="OUD09_002196"
                     /pseudo
     CDS             1..1114
                     /locus_tag="OUD09_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293527.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
     gene            1130..1279
                     /locus_tag="OUD09_002197"
     CDS             1130..1279
                     /locus_tag="OUD09_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693433.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            1360..1956
                     /locus_tag="OUD09_002198"
     CDS             1360..1956
                     /locus_tag="OUD09_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HCT9693434.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDPERTLCDIWNPWYTVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            1969..2868
                     /locus_tag="OUD09_002199"
     CDS             1969..2868
                     /locus_tag="OUD09_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HCT9693435.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNEIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVI
                     HVEEKERKLAYQRHQQMNREMER"
     gene            2871..3002
                     /gene="ssb"
                     /locus_tag="OUD09_002200"
     CDS             2871..3002
                     /gene="ssb"
                     /locus_tag="OUD09_002200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCT9693436.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGN"
     gene            3024..3356
                     /gene="ssb"
                     /locus_tag="OUD09_002201"
     CDS             3024..3356
                     /gene="ssb"
                     /locus_tag="OUD09_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCT9693437.1"
                     /translation="MIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEV
                     VADNFQLLESKAATESRAHADQSSTSPSTATFEQRNTATPNNNGLNASQNPFGGQSID
                     ISDDDLPF"
     gene            3378..3635
                     /locus_tag="OUD09_002202"
     CDS             3378..3635
                     /locus_tag="OUD09_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693438.1"
                     /translation="MAKKISLTMDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVF
                     VYLVCSYLLKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(3794..3916)
                     /locus_tag="OUD09_002203"
     CDS             complement(3794..3916)
                     /locus_tag="OUD09_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HCT9693439.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            4106..4330
                     /locus_tag="OUD09_002204"
     CDS             4106..4330
                     /locus_tag="OUD09_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693440.1"
                     /translation="MIKTQPLLVRFTLRTSHEPINCVVANIEIADKLLILEEGKYTIS
                     VTGHFNKRNQLVIESMQIRNPDHFTRLMGI"
     gene            4778..>4980
                     /locus_tag="OUD09_002205"
     CDS             4778..>4980
                     /locus_tag="OUD09_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HCT9693441.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPYTVECDCGERAKCIKSYCLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
BASE COUNT         1780 a          877 c         1042 g         1281 t
ORIGIN      
        1 gaaggggaat atgcgtatct gttggatcac ttagacgaat acaagcattt tttaaaagcc
       61 gaagcgattc cgactcctat tcatacaccg aatagtcggt atgtgaataa taagaaagtc
      121 caggaacatt acgcgatcat tcctacaaaa acggttatga cagcagcggc ttttgaacaa
      181 ctaagtccac tgcagcaggc tatttatgaa caagtgttaa aaacaacggt ggccatgttt
      241 gcggaaaagt atacctatga agaaacaacg atcttgacgc aagttcagca gcttcagtta
      301 aaggcaatcg aaaaggtgcc gctagatcta ggttggaaga agttatttgg aaaagagagc
      361 gacggaaaag agaaggaaga agagccgcta cttcctaaag tgacaaaagg agaaatggtg
      421 acggtggatc tacaagttct tgaaaaagaa accaagccac cacaacctta taccgaaggt
      481 acgttgatca ctgcaatgaa aacagctggg aaaaccgtgg atagtgaaga agcgcaatcc
      541 atattgaaag aagtggaagg aatcggtaca gaagcgacac gggcgaatat tattgaaacc
      601 ttgaaacaaa aggaatatat caaggtagag aaaaataagc tcgtggtgac gaataaaggg
      661 attttgcttt gccaagccgt ggaaaaagag ccgctgctta cgagcgccga aatgaccgcc
      721 aaatgggaaa gctacttgtt aaagatcggt gaacgaaaag gaacacaaac cacttttcta
      781 gccaatatcc aaaaatttgt ctctcactta ttggaagtcg taccagggca aatccaatcc
      841 actgattttg gatcgacgtt acaagaagtg aaagcagcca gtgaaaagca agaagccgcg
      901 cgtcatttag gaatttgtcc aaaatgccag gaacaagaag tcttactcta tcaaaaggtg
      961 gcagcttgta cgtctgaagc ctgtgatttt aaaaggtcag aagggttcat ttgaagcaac
     1021 gattgtttta aaagaagact tcactacagg ctttgaattt tctgaaaaga aaaaaacgaa
     1081 cttcaagaaa agaacacgcc gcactgcaaa ataatggagg gttaatctta tgcgtaatac
     1141 cacacacaaa caacaagaaa tcgaaaatca aaaagccaaa attcgttcag aacaggaaaa
     1201 aatgcgtgcc agcaaacgaa gactgcagca attgaagaac cagcagagga ttcaaaaaga
     1261 aatcgaatta gatcgataaa atcttcctaa ctattgtata cgtataaact tatgttatac
     1321 tgtttttata agtttatacg tatacaaagg agggagaaaa tgacaaaagc aatggtatac
     1381 aagctgctag aagaatttaa tggaactttg gctttgaagg atgccaaaaa agcgggcatt
     1441 tctcctgtaa cgatcaaacg tatggtagat cgaggggagc tggatcgaga gtatccaggt
     1501 tttttcacct tacctggtca atttcctgat gaattgttta tggcgcaaaa aaaatatgaa
     1561 cgcggcatta tttcacatat taccgcgtta gatttgtatg atttaacaga tatgatccct
     1621 agacagatcg atctgactgt accttacggt tatcacgttt ctgaaaaagg attgaaggaa
     1681 ttcgcagttg agctacacta tacaaaagcg gaatggtatg aattaggtaa gattgaaata
     1741 aaaagtcgct atgggaatcc tattatagct tatgatccag aaagaacttt atgtgacatt
     1801 tggaatccct ggtataccgt tgaggatgaa atcaaagtaa aagcaatcaa aaactatatg
     1861 gagtcggaca gaaaaaatct tagaaaattg aatgagtatc gacgaatttt accaacagac
     1921 aaaacgatgc gttcttatat tatggcttta aattaaagga gaagatacat gacacctacg
     1981 cagttaaaag caaaagtgaa gaatatttct agagaaaaag aagttgatcc gcagcttgtt
     2041 ttaagacatt ttatgatgga aaaattcttg gaaaaaatct cagagtctcc ttatcgtgaa
     2101 aattttgtgt taaaaggcgg cttcttgatt ggctcaaagt atggcattga aaatcgcaca
     2161 accaaagata ttgatacgac tttacgggaa atgaaagtaa ccaaagaaac attaacaacg
     2221 gttctaaacg aaattttttc aacgccgacg aaagaaggca ttcaatttga gattcaagga
     2281 atgaaagaaa cgagagaagc cgattactat ccaggattta gcttacgtgt gctggcacat
     2341 ttggaaaaca tgcggccaga ttttaaggta gatgtgacta caggtgactc gatctatcct
     2401 gcaacgatca ctcacagtca taaattgatg tttgaggatc gaacgattcc tttggaatcc
     2461 tatcctacag aacaaattat tgctgaaaaa ttaagtgcaa cctttgattt tttaacagat
     2521 aattcgagag gaaaagattt ttatgatctc tatactattc ctaaaatgga gaagatcgat
     2581 gagaagacct tgaaagatag tgtgagaaat acatttacac gtcgaggaaa aactgatccg
     2641 ttaaagatat actatgaaaa ggatatggat gtactgaaaa atagtcacga gctaaaagat
     2701 acctggaaga aatatcaaaa aagcaatcct tatgctgcgt cgatcacgta cgatgagaca
     2761 atggattcga ttagtaattt gatggaaaaa gtgattcacg tcgaagaaaa agagcgaaaa
     2821 ctagcttatc aaagacacca acagatgaat agagaaatgg agagataaag atgattaata
     2881 atgtgacatt agtaggacgc ttaaccaaag atcctgattt acgttatacc gcaagtggta
     2941 ctgcagtagc gacttttacc ctagccgtca accgaaattt cacgaatcaa aacggcaatt
     3001 gagaagccga ttttattaac tgcgtgattt ggcgcaaacc agcagaaaca atggcgacat
     3061 tagctaaaaa aggtagcttg attggtgtag ttgggcggat tcaaacgcgg acttacgaca
     3121 accagcaagg acaacgtgta tatgtgactg aagttgtagc agataacttt caactattag
     3181 aaagtaaagc agcgacagag agccgtgcgc acgctgatca atcgtccacc agtccaagca
     3241 ccgccacctt tgaacaacgg aatacagcga cgcctaacaa caatggattg aacgcttcac
     3301 agaatccatt tggtggacaa tcaatcgata ttagcgatga tgatctaccg ttttagtttg
     3361 agaaagaagg agtaagcatg gcaaaaaaaa ttagtctgac gatggatcta ttgatacccg
     3421 taattgcatt tatagacggg atttctttgg gcgtcactga aaatcctttg gtaaatagaa
     3481 ttgcgaaagg atcactattt cttgtattcg tgtatcttgt ctgttcttat cttttaaaaa
     3541 gagcaggaaa ggataattcc ttttggcgat tgactaaagt cacagtagca gcgacagctg
     3601 ttatacttac ggctattcta ccgctttata tgtagagtta acgggaaatc gttgaacttc
     3661 ttgcgaaata ttgctataat gaacatgaaa aagaggagtt gcgctaacaa cccctcatta
     3721 acagcaccga ttaaagaacg gtggcagaat attagttatt tggaaataac cgctgacctg
     3781 ctaaagtaga cggttatttc tttttgtcat ttttaagcat atttacaatc aaactaatca
     3841 aagcaatggt aaacatacca aaaccaagaa tggtttgaat tgtctcatat gcggacaaaa
     3901 ggcttactcc tttcatagat tttagcactt acattcataa gcaccaccac ctttccagaa
     3961 gtcgccaccg tcttcaaact ttactgtctt gactattata gcatatctaa aatcgaataa
     4021 aaattctcaa ttttagctgt ttttgctata attttagcga ggtgatcgtg atggaacaat
     4081 atattggttt actcgataaa ttagggtgat caagacacag ccattgcttg tgcgatttac
     4141 attacgcacg agtcatgaac caatcaattg tgtagtggcc aatatagaaa tagcggataa
     4201 attgctgatt ttggaagaag gcaagtatac tatttcagtg acaggtcact tcaataaacg
     4261 aaatcaacta gtcattgaat caatgcaaat tagaaaccct gatcatttta cgagattaat
     4321 gggcatataa ataaaagaag agctgcagtc ctaaatgagg attgcagctc tttttgtttt
     4381 acatagaaac aagaaaaatg aaaaggcctt tttaggaagg cgcaagcgcc ttctcaattg
     4441 aaaaacctct ctctcactcc aaccgccacc gccgttcgag aagaaaggaa gtgaacgttg
     4501 cacgttcact aagataaggg gtcttgcaaa gcaagacaaa atagaaaaaa cttcctctca
     4561 ccaaaaggtt cgttcctgtt cgctatctct ttattgattt gccagaccgg caacaagttg
     4621 ccttttatca ccaaaaagcg acaaggacaa gggtaaatgg cacgctgcac gcccttgtcc
     4681 ttgtcgcttt tagttgattc attctggcca acaaatcaat aaagaaagaa ctaacgaaag
     4741 gaagtaaaac ctttcctaag aggaggcaga atcaatcatg cgaaagaaat ttgattattg
     4801 gggtgttccc ttcagcgaat tattcccaaa ctatcatgca ccatatactg tcgaatgcga
     4861 ttgtggagaa agagcgaaat gtatcaaaag ctattgtttg tatcaatgtc caacatgcgg
     4921 gaaaaaatat accttgtctt atggcgatta tattttgatt gatgagaaag gaaagaaacg
//
LOCUS       DAKBVG010000171         4937 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000171 DAKBVG010000000
VERSION     DAKBVG010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4937
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.171"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(32..1180)
                     /gene="nagA"
                     /locus_tag="OUD09_002206"
     CDS             complement(32..1180)
                     /gene="nagA"
                     /locus_tag="OUD09_002206"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCT9693442.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1256..2092)
                     /locus_tag="OUD09_002207"
     CDS             complement(1256..2092)
                     /locus_tag="OUD09_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCT9693443.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSQDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2276..3493)
                     /locus_tag="OUD09_002208"
     CDS             complement(2276..3493)
                     /locus_tag="OUD09_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HCT9693444.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     ITSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3642..3821)
                     /locus_tag="OUD09_002209"
     CDS             complement(3642..3821)
                     /locus_tag="OUD09_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693445.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            3876..3959
                     /locus_tag="OUD09_002210"
     tRNA            3876..3959
                     /locus_tag="OUD09_002210"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3910..3912,aa:Leu,seq:gag)
     gene            complement(4199..>4937)
                     /locus_tag="OUD09_002211"
     CDS             complement(4199..>4937)
                     /locus_tag="OUD09_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693446.1"
                     /translation="ISLAELIFSLTTPGNALRAAHEINKWFPEYKNFNFLNKLDLGIS
                     SFGKPFFLDMNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFGNTMGQSF
                     TYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFD
                     NKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSM
                     SLQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         1509 a         1018 c          797 g         1613 t
ORIGIN      
        1 aagtttagtt ttaaggttaa cttaaattat attatgcgtg gaaacgttcc acgccatcta
       61 gataagttgc ttccaatgtc atatcagggt tcaatacgat aaagtctgca tcgcgtcctt
      121 gagcgatttt tccacatgta tcatcgattt tacagctgat tgctggaatc agactagcca
      181 tcatgactgc ttgttctggt gttgctaatc cccaatctac aacattttta attgcttctt
      241 tcaatttcaa tatacttcca gctaagttgc cagattctag acgtgctgtt ccttctttta
      301 ctactactgg aaattcgcct aaattgtaat ttccatcagg catcccaccg gccatcatac
      361 agtctgtaat taatgcgaca tgatcatgtc ctgctttttc cattaggatt cccgcagcat
      421 ttggatgtac atgatggcca tcacaaatca attcagaaaa gacatgttct aaagataata
      481 acgcacccac catacctggt tcgcgatgat tcaatccacg cataccattg aaagcatgga
      541 caaatacact ggcgccagct tcaacagctt tttgcgcttc ttctagcgta gcgttactgt
      601 gacctagaga aacaactact ccttcttcag tcacttgttt gacaaactct tctacacctt
      661 gacgttccgg tgctaaagca atttttttga ttaaaccgcc tgaagcttct tgccattcat
      721 ggaaagtatc caaatcagga tccccgaaat aacttgggtt ttgtgcccct ttgtgttctt
      781 ctgtgaagaa aggaccttca aaatagattc ctttgatttt agctcctggt gcttcctgat
      841 acattttgcc gattgtctca gctacatctt ttaaacgttc tttgcttgac gtcaatgttg
      901 ttggtaaaaa tgaagtcact ccacaagata acagaccttc agacatcact ttgatgcctt
      961 ctgcatcatt atccattaca tcatgattca tatagccgtg aatatgtgta tctaccaaac
     1021 caggagcaat ccatttgccg ctttggtcaa tgatgttgac ttctccctca ggtagttctt
     1081 tggtgtattc accaaaaagt ccatcggtaa tttcaagata gcctgcatgt ttgacacctg
     1141 acgctaaaaa gaatttgtca gcatagataa aagttctcat tctattctct ccctttgttt
     1201 ttctacctct aaagtactct tatttagaga agaagtcaag aatggtctac accatttaca
     1261 aattttttga aatttgtttc acagcttgtg ctaagccttc atagaatgct gctctttctg
     1321 gttcttcttt acgcttgtat attaagcttt taagcacaaa taaatcattg aggtaatagt
     1381 agctattctt ctcttgagcc caatagattc cttgattcac ctgttctaac gcttcgtcga
     1441 tttcattttg atggaataag aaatttccat aattataaaa aatttgcgaa aattcacttt
     1501 ttgatcgttc gtcagctgat tggtgcaaca gttgaatgct tcgactcaaa taatagcgtg
     1561 cttcagccgt ttttcctgca acaccatata tttttccaat gcaactgatc agttgtattt
     1621 ctgtgtcaga aacatggatt ctttcttttt ggctagagta gcttaatgct tctttcaaat
     1681 aataaaggga ctgagagata ttttgacgca acgtgaattc gcaaacaccg agataataat
     1741 aatagcgttg aaagtcagtt gcttgataca gattttcaga aatatcagaa gattttagta
     1801 cttgttctaa cttccgataa tcacgttttt ggaaacaact atccaacagt tcaaataatt
     1861 tatcgtttgt ccgagtagtt tttcgagaga gattcatcac atgatcaatc gttaccccta
     1921 gccgttgaca aatctgcatc atcaccaata cattcggcag ttcctcatta ttttcgatcc
     1981 gacttaatac actttgtgaa caaatatctt gcgaaagcat tttttgcgtc attttttgtt
     2041 cttttcgaat ctctttaatg atcgtgccaa aggactcgat taacgtttcc ataagtatcc
     2101 tccccaaaat atgcgtattc gcattctatt cgtattttac tttaggaagt cgcaaacttc
     2161 aagaaaagac ttgcaactaa ataatatact ttagttattt aagaaaactc cactagaaag
     2221 agagcctcag tgtgtttgtc tgtggccctc ctactgaaaa atttggtttt taaaatcatt
     2281 tttggcgata ggttgctagg aattctctca ttttcttagc tgtttgtttc aaatcatcca
     2341 ttttaggaag ataaacgatt cgaaaatgat ctggctggtt ccaattaaat ccaccaccat
     2401 gtacaagtag aatatgatgt tcatgtaaaa agtccaaaac gaatttttca tcatccaaaa
     2461 tattgaatcg ctttgtatca atttttggga atatataaaa agcagctttt ggttttacgg
     2521 ctgaaagacc aggaatatca ttgattgcat tatagatata ttctctttgt tcatagatcc
     2581 ttccgcctgg caggagcagc tcatcgacac tttgataacc gcctagcgcg gtttggataa
     2641 tttgctgaga caatacattg gaacacaaac gcatggagga caacatattc agcccttcta
     2701 tatagccttt aacgtgtgac ttatctccac ttaggaccat ccagcctact cgaaatcctg
     2761 ctacacgatg agatttggat agaccattca gcgtaacaac gaacaaatca ggcgctaatg
     2821 tagcaatcgg aatatgttct agaccatcca tcactaagcg atcatagatt tcgtcggaat
     2881 aaataatcag gttattttgt cgagctactt caacgatttc ttctaataat tcttttggat
     2941 aaagtgcccc agtaggatta ttaggattaa tgataacaat agcttttgtc cgactcgtta
     3001 tttttgatgt gatatcatca atatcaggat accattctgc ctcttcatcg caaatataat
     3061 gaacaggtgt gcctccagct aatgcaatag aagccgtcca caacggatag tccggcattg
     3121 gaaccagtac ttcatctcca ttgtccagca acccctgcat acacatcgtg atcaattcgc
     3181 tcactccgtt gccggtgtag atatcattga tcgtcacatt cggaaaatgt ttcagttggc
     3241 aatattgctc aattgctttt ctagcagaaa agatcccctt ggaatctgag tagccttctg
     3301 agtcacgggc attcatgatc aaatcacgaa tgacttcgtt tggtgcttca aatccaaaag
     3361 gtgcgggatt tcccgtgttc aattttaaaa tactaatgcc ctcttcttgc atccgctccg
     3421 cttcttctag aacgggaccg cgaacatcgt aactgacacc ttctaatttg ttggattttt
     3481 caaattctct catcacttag ccaatccctc atttcaaaaa gttagaactt tccaaatttt
     3541 ttgttattta ctatttcaat atcatactcc atcctagaaa attttgccac ttatttagga
     3601 aaaacgtaag tattaaaaaa acacaaacaa gcgttaagaa cttaattgtc atgatagtca
     3661 ttccatagat tcgctgccca atccattgca attccacaaa tgattcctag cataaaacca
     3721 agcaaaaaac gtccgaatgc aagtccaatc aatacaccta agatcattcc cagtcctgaa
     3781 aattctccgt catatttctc catcttaata agtcctccca tttttgagct tgacaactat
     3841 gtaaaacctc gctatcatta ttgtgttatt attacgcaga tgtgttggaa ttggcagaca
     3901 agcatgattg agggtcatgt gagcgttagc tcgtgtgggt tcgagtccca tcatctgcac
     3961 tacaactcgt gaagttaatt ttgcttcacg agtttttttg tttgaacgtt tctttatgcc
     4021 attatataca tatcactgta tcctttacaa aaagaacgta caatcatgta aacagcctga
     4081 ggtgagtata tgaaggtcgg aaacaagcta ctcgtagaaa cttgcaagtg ttaaaacaat
     4141 taatcgaaaa acgagtaaaa tccctcacct aatactagag cgagggattt tttgtatatt
     4201 atctgtttat caccgttatt ataaaggtac aaataccaag cacggttaat ccaaattgca
     4261 taaattcatt tttttgtaaa ctcatgcttt tcattagctc tttaactgcc attagtacta
     4321 agatggcgat aacaacataa agtatatagt acgtccgcat tccggaagcc catactgtgg
     4381 gagaaaatcc cataattaac cttgaacaaa atcccattat catcaaaata actaaaaaaa
     4441 tagaagtttt cttattgtca aaacttaaat aaattccaac aattaaacat agtaataatg
     4501 ccaagactac taaagtagct atccaagtac ctggataaaa aagtgacagt ttcgttccta
     4561 actctgtaaa gagattattc aggttactac tactccaaat cattgccctt ttatttccat
     4621 tgacgtaagt aaagctttga cccattgtat taccaaaata aataatcagg tttaagaaaa
     4681 acggtaatgc agtcaatata cgcacataat agttttgaca tttcctatat gttagaagga
     4741 aaattaagaa aaatagcaat aaaaataaga tattcatgtc taaaaaaaac ggctttccaa
     4801 atgatgaaat acctaaatct aacttattaa gaaagttgaa atttttatat tcaggaaacc
     4861 atttattaat ttcatgtgca gcacgtaatg cattccctgg agttgtcaaa gagaaaatta
     4921 attctgctaa gcttatt
//
LOCUS       DAKBVG010000172         4911 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000172 DAKBVG010000000
VERSION     DAKBVG010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4911
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.172"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            16..834
                     /gene="purR"
                     /locus_tag="OUD09_002212"
     CDS             16..834
                     /gene="purR"
                     /locus_tag="OUD09_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HCT9693447.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            1337..2710
                     /gene="glmU"
                     /locus_tag="OUD09_002213"
     CDS             1337..2710
                     /gene="glmU"
                     /locus_tag="OUD09_002213"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCT9693448.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            2804..3775
                     /locus_tag="OUD09_002214"
     CDS             2804..3775
                     /locus_tag="OUD09_002214"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCT9693449.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      3781..3883
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3949..4518
                     /gene="thiT"
                     /locus_tag="OUD09_002215"
     CDS             3949..4518
                     /gene="thiT"
                     /locus_tag="OUD09_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HCT9693450.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(4599..>4911)
                     /locus_tag="OUD09_002216"
     CDS             complement(4599..>4911)
                     /locus_tag="OUD09_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693451.1"
                     /translation="ILSDKINVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQ
                     VGLIDGKYRIPIYVIEDYIWKLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPL
                     K"
BASE COUNT         1594 a          868 c         1076 g         1373 t
ORIGIN      
        1 aaggagttgg aataggtgaa agtacgtcgc agtgaacgtc tagttgacat gacacagtat
       61 ttattgaatc acccgcatga attgatttcc ttaacaagct ttgccgaacg ttatgaatct
      121 gctaaatctt ctatcagtga agatttggga atcatcaaga aaacatttaa agaacgaggg
      181 tatggaacct tggaaacaat cccgggtgct gcaggtggtg tattgttcat ccctgaaatt
      241 tcatatgaag aggcgaaaaa atatattgaa tctctagcag aacgtctatc agaacaagac
      301 cgtctgcttc caggaggata tgtttatctc tctgatttgt taggagaacc tgaactgctg
      361 cgtcaagttg gaagaatcat tgcttctaaa taccttggtg aaaaaatcga tgctgtaatg
      421 actgtagcca caaaaggcgt acctattgcc caagctgtgt cctattatct gaatgcgcca
      481 ttcgttattg tacgccggga ctcaaaaata acagaaggat caacagtaag tgtcaactat
      541 gtgtctggat cttctgaaag aattgaaaag atggaactgt ctaagagaag tttgaaacgt
      601 ggttcacgtg tccttgtagt ggatgatttc atgaaaggcg gcggaacagt caacggaatg
      661 aagagcttga ttgaagaatt cgaatcagaa cttgttggca tcactgtatt tgccgaatca
      721 aaattcaacg gtcatcgcgc catcgatgat tatacgtcac tgctttatgt caaagatgta
      781 gatactcaaa cgaaaaatat tacggttgtg ccaggaaatt atttttcaga ataagaggtt
      841 gtgaaaaagc tgcttagttt cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat
      901 tttttcataa atttggctta ataccgagaa actggctttt gaacaccgtt tatctagagg
      961 ttgtgaaatc agcgtcctgt cctgagtatt agctcaataa acggaaaaat tgctcctcat
     1021 attttgccgt ttattgagct aataccgaag gtcagctgat tgaaaaacgt ttaatgaaga
     1081 ggttgtaagc ctaccgttta actctaagtc acaagaaacg atttcaaaaa acagcttttc
     1141 atgttttatg agtagtgtga cttgtgaccg aggagttggc aggagagcac cggttaccaa
     1201 atagataggc ccgttatggt ctatctattt ttttatccag aaaacgctgg gataaaagaa
     1261 aatcacttgt tttgttgaaa aaatgggtat actgggataa actgaaatca gcaaactata
     1321 tttaggagtg acgcagttgg acgcacgtta tgcaattatt ttggcggcag ggaaaggtac
     1381 ccgcatgaag tcaaaattat ataaagtttt acatcccgtt tctggtaaac ctatggtaga
     1441 acatatcatt aatcgggtca gtgaaacgaa accagatgaa gtgattacga tcgttggaca
     1501 cggagcagaa caagttaaag cacaactagg agaacgcagt aaatatgcgt tacaagcaga
     1561 acaattggga acaggacatg ctgttttgca agctgcttct ttcttggaag gaaaaaaagg
     1621 aacaacgtta gttatcagtg gcgatacacc attattgact actgaaacgc tgaacaatct
     1681 ttttgaatat caccaaggaa aaaatgcaag tgcaacgatc ttgacggcac aagcagaaaa
     1741 cccaacagga tatggacgaa tcatccgtga ccatattggg atcgtagaaa aaatcgtgga
     1801 acaaaaagat gcaacaccag aagaagctct tgtacaagaa atcaatacag gaacttactg
     1861 ttttgacaac gaagctttgt tcgatgcgtt aagcaaagtt ggaacaaaca atgctcaagg
     1921 tgaatactat ctaacagata tcatcgaaat cttaaaagaa gaaggccata cagttgctgc
     1981 ttatcaaacg gatgattttg aagaatcaat gggagtcaat gatcggatcg cgttagctaa
     2041 agcaaatgag atcatgagaa agcgaattaa ccagatgcat atggtcaatg gcgtaagctt
     2101 tgtcgattca gcaacgacat acatcgatgc aggcgtagaa atcggtccag acacattgat
     2161 cgaagcaggt gtacaaatcc aaggaaatac agtcattggt tctgattgtg tcatcgggtc
     2221 acattcaaaa atcgtagaca gtaggattga ggatcacgtc gtcattgaaa actcagtcat
     2281 tgaaagcagc catgtcaaga aacatgcaga tgtgggacct tatgcacatt tacgaccaaa
     2341 agctgaaatt ggcgaaaacg tccatatcgg aaactttgtg gaagtgaaaa atgctcaaat
     2401 cggcaaagga actaaagtcg gtcacttgac ttatgttggt gatgctacgc tgggagaaga
     2461 aatcaatgtc ggatgtggtg ttgtcttcgt caactatgat ggaaagaaca aacatcatac
     2521 gactgtcggc gaccacagct ttattggttc tagcacgaat atcatcggac ctgtcgaggt
     2581 agctaaaaac agttcgattg ctgccgggtc aacaattaca gacaatattc cagaatatgc
     2641 gctggcaatt gcgagagcaa gacaagtcaa taaagaagga tatgcaaaaa aactccctta
     2701 tctaaactaa gacttttgca aaatcacttg atttatttaa aaaatatgac tactatttat
     2761 taggtagaat tctagagttg tgaaaaagaa agtggaggtc cccatgtcaa aacattactt
     2821 tgacccaaga ttaaaaattt ttgcgttaaa ttccaatcgc ccattagcag agaaaattgc
     2881 agcagcagtc ggcgtcgagt taggaaaatc atctgttact caatttagtg atggagaaat
     2941 tcaagtaaac attgaagaaa gtattcgtgg atcgcatgtc tatgttattc aatcaacgag
     3001 cagcccagtc aatgataact tgatggagtt gctgatcatg attgacgcac tgaaacgagc
     3061 aagtgcaaaa acaatcaacg tagtcatgcc ttattatggc tatgcgcgtc aagaccgtaa
     3121 agcacgtgca cgggaaccaa tcacagcgaa attagtagca aacatgattc aaaaagctgg
     3181 tgcaacaaga atgctgactt tggaccttca tgcagttcaa atccaaggtt tctttgatat
     3241 tccagtcgat catttgatgg gtgcgccact tattgccaat tatttcttag aaaaaggaat
     3301 caaaggcgat gatgttgtcg ttgtttcacc agaccatggt ggtgtcacac gggcacgtaa
     3361 attagcggaa tttttgaaat ctccgattgc gatcatagac aaacgccgtc caaaagcaaa
     3421 tgttgcagaa gtaatgaata tcattggaca agtcaaaggc aaaacttgtg tactgatcga
     3481 tgatatgatc gatacagcag gtacgatcac attggctgcc aatgctttga aagaagctgg
     3541 tgcaactagt gtgtatgctt catgtacaca tccagtatta tccggccctg ctttacaaag
     3601 aatagaagat tcagctatcg agcgtctagt tgttacagac tcgatctatc ttccagaaga
     3661 tcgtaaaatc gacaaaatcg atgaagtaag tgttggcgga ctaatgggag atgcaatcaa
     3721 acgtatccat gaaaacaaac cagttagtcc attgtttgaa acaaaaaata aataataaat
     3781 aaactcacaa aggggagtcc aattgggctg agattgaatc tatttctaaa cccttcgtac
     3841 ctgtatcggt tatgcgagcg taggaattgt gaataaacag ctgtttgact attttcgaaa
     3901 gctgcttcct cctttgtgac ttttttattc aaaggaggaa gtttttttat gggaaaaaat
     3961 agagtttggg tagaaggaac gattgttgca gcgttagcga tggtgttgtc gttgattccg
     4021 atccagatcg gcagcagttt ttctatttca ttagggcaga ttccattgac tctttttgcg
     4081 ttaagaagag ggacaaaggc agggatgctg gcaggtttca tttggggcat cctacatttt
     4141 ccgttgggac aagtttatta tttaagtgtc gttcaagtgt tgattgagta cccgattgcc
     4201 tatacttttg caggcgcagc agggctcatg gcttcaaatg tccaaaaagg ctatgtagac
     4261 gataatacga aacaaattag aaaaagcatc gtgtttggtg cattgattgg cgcactttcc
     4321 agatattttt ggcattttat tgcaggagtg gttttctggg gagcgtatgc actatggggg
     4381 atgaatcctt ggctcttttc tttcgtaatg aatggtgcca gcgggttgag tacagctatt
     4441 gtaacgatca tcgttttatt agggatccaa caaacgactc cttcgatttt tgtgccagat
     4501 aaacgacagg agctgtaaat aaaaaagaag caagaaactt gagacagaat catgtcctag
     4561 tttcttgttt cctttttatt ttttgataag aaatagatct acttcaatgg cagataagaa
     4621 agatcggcat cttctaccaa ttgccagtgt cgatcggtga cagagtagat taaagtagcg
     4681 atgatttcct cttctcgata ccgggtgcct tttaatttcc aaatataatc ttcaatcaca
     4741 tagattggga tacgatattt accatcgatc aaaccaactt gatcaaaaga aaaaatgatc
     4801 ccttcctgca tcaaattacg catttctttc aataattcca cactgatttt ttcaacaggt
     4861 gtttcttttt tgaaaaaaga tttttttaca ttgatcttat ctgacaagat c
//
LOCUS       DAKBVG010000173         4905 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000173 DAKBVG010000000
VERSION     DAKBVG010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4905
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.173"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            62..949
                     /locus_tag="OUD09_002217"
     CDS             62..949
                     /locus_tag="OUD09_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HCT9693452.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1089..2015
                     /gene="cysK"
                     /locus_tag="OUD09_002218"
     CDS             1089..2015
                     /gene="cysK"
                     /locus_tag="OUD09_002218"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCT9693453.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2251..2697
                     /locus_tag="OUD09_002219"
     CDS             2251..2697
                     /locus_tag="OUD09_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCT9693454.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2970..4322
                     /locus_tag="OUD09_002220"
     CDS             2970..4322
                     /locus_tag="OUD09_002220"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HCT9693455.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4493..4780
                     /locus_tag="OUD09_002221"
     CDS             4493..4780
                     /locus_tag="OUD09_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693456.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1683 a          813 c         1029 g         1380 t
ORIGIN      
        1 ttaagtttgt ttaaagaaga gaacttttta ttaagttttt ttattttgag gaggaatttg
       61 aatgcaagag ctgaagaccc gcagtgcccg gcaccataga cctaaaagaa aaatggtgat
      121 caataaaaaa gcgattgctc ctttaattgg aacgagtctt ttagttgggc cgatgatggt
      181 tacagcggta ccgcaacatg ttcatgcaga tgaacaagga tctcaaacag tcgctgatcc
      241 tcaagcgttt atcgatcaaa tcggctggtc tgcgtcagaa gttgctgcaa gtaatgactt
      301 gtatgcttca gtcatgatcg ctcaagcgtt gttggaaagt gcatatggca cttccggatt
      361 agctagtgcg ccaaattata atctattcgg tgtgaaagga agctataacg gacaggtcgt
      421 ttatatggca accaaagaat atgtaaacgg acaatgggta acgatgaatg agccattccg
      481 aaaatattct tcttattggg aatccttcca agaccatgcg aatgttcttc gttcaaccag
      541 tttttccacg ggcgattatc attatgctgg cgtgtggaaa agcaatacaa caagttttta
      601 tgatgcaaca gcgtacttga caggtcgtta tgctaccgat ccaaattatg gggccaaatt
      661 gaactcattg attaatactt ataatttaac ccgttttgat acgccatcaa caggttcggc
      721 tacaacaacc acaagtacaa caacaacgac tactacgacg acttcaacgt catcatcttc
      781 aacaacagat gtaaatacaa ataatggtca gacatataca gtcgtgtcag gtgatacgct
      841 atgggatatt gcacaaaaat ttggcatgtc tgttgatgat ctaatggcga aaaatggcat
      901 gtctatgtcc agttattctt tattagttgg tcaagaacta aacgtttgat ttagataaaa
      961 aggcaaagaa attaccatat atggtatatc tttgtctttt ttttgctagt caaagacgaa
     1021 tcactcgcca taaatgaaca gattgtatta tgataagtaa gaagagcaaa gacaaggagg
     1081 aattgaagat gacagctatc tataactcag tcaccgaatt gattggcaaa acgccaatcg
     1141 tcaaattaaa taaaatcgta ccagaagatt cagcagatgt ttttgtaaag cttgagtttt
     1201 ttaatccagg aggaagtgta aaagatcgta ttgctttaag tatgatagaa aaagcagaac
     1261 acgatggtct actgaaacca ggtgatacta tcattgaacc tacttctgga aatacaggaa
     1321 tcggattgtc gatggtagga gttgcaaaag gatataaagt aatcatcgtg atgcctgaga
     1381 cgatgagtat cgaaagacgt ttattgatga aagggtacgg cgcagagttg attttgactc
     1441 ctggtgcgga tggtatttct ggatcaatca gagaagcaga acgtttggca aaagaaaacg
     1501 gctacttttt accattacaa tttgaaaatg aagcaaatcc tcttgtgcat gagaagacga
     1561 ctggtcctga gatacatcaa gcatttggag taaatggctt ggacgcgttt gttgcaggta
     1621 tcggtacagg aggaacgatc actggcgctg gccgagaatt aaaacgtgtg tatccgaaaa
     1681 tcgaattaat cggggtagaa ccagcagaat ctgctatttt agaagggaaa gaagcgggac
     1741 ctcacaaaat ccaaggaatc ggtacaggtt ttgttcctaa aacactggat acttctgtgt
     1801 atgataaagt cctttctatt agtggagacg aagcgatgga aacagctaga gaagttgggc
     1861 gaaaagaagg gattttagta ggcatctcat ctggcgctgc aattgctgcc gcgctaaaag
     1921 tcgcaaaaga attaggaaaa ggcaagaagg tattagctgt ggttcccgac aatggtgaac
     1981 gttatttatc tacagccctt taccaagaga tataaggcac ttggtttttc tccagatagg
     2041 tttatgtaaa aaacttttaa agatgaaaag aacacacaaa aatttatgat gagaaaaatt
     2101 atttgaatag agacgtagaa tagtgaacgc gactgtttta cgtctttttt cattaaaata
     2161 caataaaatt gaattttctt ttcatgttaa gtggatttta aaatgtaaaa atagtatgat
     2221 aagacgagaa taacaacgtg gggtgtttac atggaaaata agttagtcga agaggttatc
     2281 gaaaagttaa aaaaagagaa cattcgaatc acacctcaac ggtacgcggt tttagaatat
     2341 ttgattgaac atcattccca tccaacggct gatgagattt ataaagcact tgaacatcgg
     2401 tttccaaata tgagtgttgc gactgtttac aataaccttc gcttatttac ttcgattgga
     2461 tttgttcaag aaatgaagta cggcgatgct tctagccgat ttgatttcag ttcaaagcgt
     2521 cattatcatg taatttgtga aaactgcggt aagattgttg attttcatta cccagggctt
     2581 gaagatatag aaatggctgc aagcagactg actgattttg agattaatga acatcggttg
     2641 gaattgtatg gactctgtcc ggattgtaaa gagtcagcaa aaaaggcgca aatgtaaaaa
     2701 cagtttctac aagtgttata cctaaaaaaa gctagctgtg taaaaaaaac gctagctttt
     2761 ttattttttt gtcttttatt cacaatctta gatataaaag tgaatattat taaaataaga
     2821 atcttgtaga tattgggttc ttatgcttga aaaggaatga aactacaaac aaacctttgt
     2881 gaattcacaa gcattaaact gttgtatccg ttttatttct gttatagtag ttttgtcgaa
     2941 ggacaaaatg ttaatcggag ggaccataaa tgaaagtagt agtaattggc tgtactcatg
     3001 caggtactgc agctgtaaaa agtattttaa aaaatcatcc agaagcagaa gtgacagtat
     3061 atgaaagaaa tgacaatatt tctttcttat cttgcggtat cgcactttat gtcggcggag
     3121 tagtaaagga cccagcaggc ttgttctact caaatcctga agaattatct tcattaggag
     3181 caacagttaa aatggaacac gatgtaacag acgtcgatac agaaaataaa acagtaacgg
     3241 caaaagatct gaaaacagga gaaacagaaa cggtttctta tgataaatta gtgatgacaa
     3301 ctggttcatg gcctattatc ccaccgatta gtggaatcga tgcgaaaaat gtactcctat
     3361 gcaaaaacta caatcaagca aatgaaatta ttgcacaagc gaaagaagca aaacgtgtag
     3421 taattgttgg tggtggatat atcggtattg aattagtaga agcttttgtc gaatctggaa
     3481 aacaagtgac attgattgat ggattagatc gcatcttgaa taaatatcta gataaaccat
     3541 tcacagatat cttagaaaaa gaattgacag accgcggtgt tactcttgcg ctaggcgaaa
     3601 atgttactga atttatcaca gatgaagaag gaaaagtgaa acaagtagct acaccaacag
     3661 atacatttga tacagatatg gtcattttat gtgtgggctt ccgtccaaat acaaaactga
     3721 tcgaaggaaa agtagataca ctgccaaacg gggcaatcaa agtgaatgaa tacatgcaga
     3781 caagtaatcc agacattttt gcagctggag actctgcagt agtcaactac aatccaagtg
     3841 gcacgcaaaa ctatattcct ttggcaacaa atgccgttcg tcaaggtttg cttgtgggaa
     3901 ataacttgac agatcataaa atggcgtatc gtggtactca aggaacttca ggactttacc
     3961 tatttggctg gacgatcggt tcgacaggtg tgaccaaaga aagcgcaact ttgaacgatc
     4021 tagaagtcca agcgactgta tttgaagata attatcgtcc agagttcatg ccaactactg
     4081 aaaaagtaat gatggagcta gtctatgaaa aaggaacgaa tcgtatcgta ggtgctcagt
     4141 tcatgtccaa atacgacatt acacaatcag caaataccat gtctttagct gttcaaaata
     4201 aaatgactgt agaagatctc gcactttcag atttcttctt ccaaccacat tttgaccgcc
     4261 catggaatta cttgaaccta cttgcacaag cagcgctagg cgaaatcgaa actgcacaat
     4321 aaagaaaaag ggttgtgaca caattttgtc acgaccctct gtcttttttt gtgaaaagta
     4381 caattagttg tctttcttcg aattttttgt cacaataagc agcaaagata aagctgttac
     4441 ataaaagaac aagcagcttt atttgatttt gaaaagaaag gtgagaaaga agatgaataa
     4501 agaatatttt gtccaacaag atgcattttt agaagattat aaaagtgctt cgaagaaatt
     4561 aacgatcaat attgatgagt tggcagatca agtactctct cagttgaatg aaacgcaaga
     4621 ggaatattac acgatgccag caaatgacgc caaagataat gtttctcatc gttttccttt
     4681 cagtagacgc atctactcag atgaaaatga cggcacattg aatacagagt acacatatga
     4741 aggtcagcca tatgtgctag aaactgatga tgatctttaa agacagtttt tgaaaaggcc
     4801 gctcatcctt aagtgatgac aaaaaatcat cacaattacc gatgactgtc tctttactat
     4861 cttttaatca gaaaaagggg ttatgacaag ctttgtcaac ccctt
//
LOCUS       DAKBVG010000174         4904 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000174 DAKBVG010000000
VERSION     DAKBVG010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.174"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            <1..742
                     /locus_tag="OUD09_002222"
     CDS             <1..742
                     /locus_tag="OUD09_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCT9693457.1"
                     /translation="IMANSFKPLKDAYQTITSLIPQPFTLENFCYLFENLPIIKITRN
                     TFFIATIITVVKLGVAFFAAYSFTYCRIKGKKIWYFIIIGTMFIPFTVAMIPNYLLIA
                     KLNLIDTTWGVIIPQFADAMGIFLLTQTMREIPVSLLDVAALDNIKQTTVMSKIVFPL
                     TRHAITSTGIWFFITSWNEYVWPVLILRTVDNYTLPLAMQTYISSEGGTNFTVAMAIS
                     LITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            758..1882
                     /locus_tag="OUD09_002223"
     CDS             758..1882
                     /locus_tag="OUD09_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT9693458.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            1860..2663
                     /locus_tag="OUD09_002224"
     CDS             1860..2663
                     /locus_tag="OUD09_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCT9693459.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            3114..3386
                     /locus_tag="OUD09_002225"
     CDS             3114..3386
                     /locus_tag="OUD09_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HCT9693460.1"
                     /translation="MEKMAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQ
                     GKIPFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            3376..3705
                     /locus_tag="OUD09_002226"
     CDS             3376..3705
                     /locus_tag="OUD09_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCT9693461.1"
                     /translation="MNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSKYH
                     TKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQRL
                     EERMEEL"
     gene            complement(3804..>4904)
                     /locus_tag="OUD09_002227"
     CDS             complement(3804..>4904)
                     /locus_tag="OUD09_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HCT9693462.1"
                     /translation="KKETIVRFEQYNHMPLVTRLKKQRYTCKNCRTHWTAQSYFVRLS
                     HSIADHVRYKIASLLTEKVSLSFIAKSCQVSLTTVTRTLKEFKSYLPKPSKKILPRVL
                     MVDEFRSHASIEDKMSFICADGETGNLIAVLPTRKLPRLTSYFLGCTNPEEVEFLVTD
                     MNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQALNELRIREMNELRKAGQKSQAEKLKK
                     NWRFLLKNRASINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSAPLKEAYPFFHELV
                     EAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITSAMIYPYSNGKIEAKNT
                     HIKTMKRVSYGFKSFENMRIRIFLINQLINVR"
BASE COUNT         1549 a          812 c          981 g         1562 t
ORIGIN      
        1 tattatggcc aattctttta agccattgaa agatgcctat cagacgatta ctagtttgat
       61 accacagccg tttaccttgg aaaatttttg ctatttgttc gaaaatcttc caattatcaa
      121 aattacgaga aatacttttt ttattgctac aattataacg gttgttaagt taggcgtagc
      181 tttttttgcg gcatatagtt ttacttattg ccgaatcaaa gggaagaaaa tctggtactt
      241 tatcatcatt ggtacgatgt tcattccttt tactgttgcg atgattccta attatttgtt
      301 gattgcaaaa cttaatttaa ttgatactac ttggggtgtg ataataccac aatttgctga
      361 tgcgatgggc atttttttat tgacgcaaac aatgcgggaa attccagtga gccttctaga
      421 tgtggcggca ttggataaca tcaaacaaac aaccgtcatg agtaaaattg tttttcctct
      481 tactagacat gcgattactt ctaccggtat ttggtttttt attacctctt ggaatgagta
      541 tgtttggccg gtattaattt tacggacagt agataattat actttgccgt tggcgatgca
      601 gacctatatt agttctgaag gtgggacaaa ttttaccgta gcaatggcga ttagtttaat
      661 tacaatgttt gtaccgttag tcttgtatgc aatttttcaa aaatatatta tcgggacttt
      721 tgtttctgca ggtattaaat aaattggagg acaaatcatg ggagaaacgc ttgaattaag
      781 aaatatcgga aaaaaatatg gagaacatac cattttaaaa gaaattgatt tagctgtggc
      841 cgctggagaa cgaattgtgt tactgggacc atctggttca ggaaaatcta ctttgctgag
      901 aatgattgct ggtttggaaa aaattacgag tggagagctt tttttaggta cacaactagc
      961 aaataagcta ccttgtggag aacgagagat cgcaatggtt tttcaaaatt atgctttata
     1021 tcctcacatg actgtaaagg agaatattat ttttgcatta agggcgaata aaattaagac
     1081 agctgacatt cagcaccgtt tggaagaagc tttgaagatg ttaggtttaa cgccatacca
     1141 agatcgactt cctaaagatt tgtcaggagg gcaacggcag cgtgtagcat tggctcgtgc
     1201 ggtggttaaa cggagtaatt attttttatt agatgaaccg ttatcaaatt tggatgtgcg
     1261 tttacgattg gatgcacgaa aagaattggt aaaaattcat caacgttata aacaaacttt
     1321 tgtctacgtc actcatgatc aaattgaagc gatgacgtta gctgaccgga ttgttttatt
     1381 gaatgaaggt agaattcaga tgattgatac gccgaaaaat gtttatacga aacctaaaaa
     1441 tacttttact gcaaatttta ttggttcgcc aggcatgaat attatgccag ttacttatga
     1501 tcaaggaaca ttaaaggtag ccagtcaatt tttaacattg aatccagctt ggcaaaaaca
     1561 acttcttcag gttagtggct cattgtattt aggcataaga ccagaacacg taaccttttc
     1621 aaaagaaaca agcgatttac acggtcaagt gaagtatcgt gagctattag gtcaaagtta
     1681 tgcgtacacc gttaaagtcg atggtataga aatgattgtc cttaatgaaa aacctacttg
     1741 gcagattggt gatgcagtca atctccgcat agaacctgaa agaatgcatt tttttgatta
     1801 tcagacgcaa gaaaatttag gatatcctga ttattttatt gaagaaagag ggaatagcca
     1861 tgcgcttttt gcatctctct gatatccatt ttagagaaaa ctatcaaact aacttgttta
     1921 ctgctgaact atttccaaca attaaaaatc cacagttggc attggaaaaa ttgttacaag
     1981 aactctcgtt aacagtgtac gattttattc tattaacagg tgatttagta catgaagggg
     2041 ggatttctga ttatcaagtc ttacggaaaa tgttggatca atatttccca accacacctt
     2101 actatttttg tcgtgggaat catgatcgtc gcagtgtttt ctttgaagga atgagtgttg
     2161 aaagcaatga aacgggagag tatatccatt gtacgaatca aaatgggtta cgaattattt
     2221 ccttggattc tgctcaagat acccagcacg aaggaaaaat atcgatagga cagatgattc
     2281 aattacaaga gtggttgggt acacctgctg aaaagggaac gattttattg gttcatcatc
     2341 cattgtcgtg ggaagatcca tctattgtaa ctgaagttcc ggaaggtttt gctgaattga
     2401 ttgccgaaag tgatatttta ggtatttttg taggacatat ccatcaaggt agcagtgctg
     2461 aatttgctgg taaaaaacag tttatggctg aatctttatc atttggtgta gatgaatacc
     2521 cagactattc tctttttacc aatagaacag gattcactag tggcagttta attgacggaa
     2581 aaatttttac ttatcagcat tatttaactc cacatcaaat agaaataggg cgcatgaaaa
     2641 aaccaattac tggtaatcat taaaaattat tgattaaaaa taaagacttc attcactttt
     2701 tagccgtggt gctcaatgtc tggttccact tgaagaactt ggaaaatcgc tatcaataaa
     2761 ccactatccc tccaattaca aaaagggtgg agaaagtttt ccaagaacca tatcggtctt
     2821 cgtggttgtg ccattcgttt tttaccagag cataccacat ctttgtggag ttttcagaca
     2881 cgtcctagtt aatttggttc ttttaggagt cttttcctgt tctgtgtgtc gttgagtgtt
     2941 cgcttcaatt atcgctgtgg cggattttga aacttttcta tctatcaagg ccatttagca
     3001 ctatatcctt tccttatatt tagataaata cactgcattt caaggagttc aaaagtagtt
     3061 gtattaattc catttgaact ctttccgtga tataatgtgt ttgtcagaaa gaggtggaga
     3121 aaatggctaa gttacaaatt tctatggacg aaaaattgaa aaatgaagca gaaagcatta
     3181 ttgatgaact aggcatgagc cccaaaacag ccgtcaccgt tttttataag caaattgttg
     3241 agcaaggaaa aatcccattt tctattgaat tgtcggaaaa aagtaaacga atccataata
     3301 ttcaaagatt agcggaaaaa ttgcctgtcc aaaaactaga cactgacgaa aaaattgaag
     3361 aatggttcaa tgaagatgaa tactaatgat atagcaaccg tctacgttgc tttcacagct
     3421 gcagagggcg gtaaacgccg gcctgtctat attctaaaaa atggactgga cacaatttcg
     3481 tttctgtcaa ttacttcaaa atatcatact aaatccgata aaataaagct gaaatacgtt
     3541 gaaataaggg attggaaaca ggcaggacta aaaaaagaat cttggattga cgtaggaact
     3601 ctaaacgaat tacccaaaaa caacaaaaag gtcaaatgga aaaaaattgg ggaattaagt
     3661 ccaagcgatt tacagcgact ggaagaacgc atggaagaat tataataata ctaaaaagaa
     3721 gggctctttg tcaataagga ctgatgggtt gtacaaaatc aaatctgggt cagaatgaac
     3781 cacattctgg ttcagatttt ttgttatctt acattgatta attgattgat caaaaagatt
     3841 ctaattctca tgttctcgaa tgatttaaat ccgtaggata ctcgtttcat tgtctttatg
     3901 tgggtattct tcgcttcaat ttttccattg gaataaggat agatcattgc gctggtgata
     3961 ccttcttcat aggtcagaag gttttgaagc ttctcccgaa agccgtcatc taacgtttcg
     4021 ggaagttctg ccaataagga gaaaaataag tcagggtctt tgtctcgaaa ggcttcgact
     4081 aattcatgaa aaaagggata cgcctccttt agaggcgcag aaaactcaag caatcgatca
     4141 atcatcattg cttcagtgag aaatgggtat tttggtgctc ggaaactttt ccatgttttg
     4201 tactcataat ggttgatgct tgcacgattt tttagcagaa agcgccagtt ctttttcagt
     4261 ttttctgcct gacttttctg tcctgcttta cgaagctcat tcatttcacg gatacgcaac
     4321 tcattgagtg cctgattcat gtgtttgaca atatgaaacc gatcaatcac tactttcgca
     4381 tttggcagaa cacgtttggt aagctggaag taagcggcgt tcatgtctgt gactaagaat
     4441 tctacttctt ctggattggt gcagcctaag aaatagcttg ttaatcgagg taatttacgc
     4501 gtaggcaaga cagcaattaa gtttcctgtt tcaccatctg cgcaaataaa gctcatttta
     4561 tcttctatgg aagcgtgcga acgaaattca tcaaccatca atactctggg gagaatcttc
     4621 ttagaaggct ttggtaaata gcttttaaac tctttcaatg tacgagtaac ggtagtcaaa
     4681 gatacctgac aacttttcgc aataaaagac aaagatactt tttcagtcag taaagaagca
     4741 attttatatc gaacatgatc tgcgattgaa tggctgagtc ggacaaaata actttgagcc
     4801 gtccaatggg tacgacagtt tttacaggta tagcgttgct tttttaggcg cgtaaccaaa
     4861 ggcatatgat tgtattgttc aaaacggaca atcgtttcct tctt
//
LOCUS       DAKBVG010000175         4899 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000175 DAKBVG010000000
VERSION     DAKBVG010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4899
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.175"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(179..1789)
                     /locus_tag="OUD09_002228"
     CDS             complement(179..1789)
                     /locus_tag="OUD09_002228"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HCT9693463.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(2982..4322)
                     /gene="glnA"
                     /locus_tag="OUD09_002229"
     CDS             complement(2982..4322)
                     /gene="glnA"
                     /locus_tag="OUD09_002229"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HCT9693464.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(4350..4742)
                     /locus_tag="OUD09_002230"
     CDS             complement(4350..4742)
                     /locus_tag="OUD09_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCT9693465.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT         1455 a         1053 c          801 g         1590 t
ORIGIN      
        1 aattttcaag aagttttttg caaaaaataa aatatccaat agcgctttaa tcgaaaaaag
       61 tcttttgctt tctatctttt ttcctacaaa taataacaaa tcagaaaaag agaaaagtga
      121 caagaaaatt gcttatcccc ttctcttgct acttttctct ttctatcaag ttcaaaaatt
      181 attttgtttc gtgatctgct aatgatgctc cgacaaatgc tttgattaat ttttgcgggc
      241 gatttggacg agaaatcaat tctgggtgga attggcatcc cacaaagaat tttttctctg
      301 gcagctctac gatttctact aaacggttat ctggtgagac acctgaaaag actagccctt
      361 gttcttcaaa caattgacga tatttgttat tgaactcata gcggtgacgg tgacgttctt
      421 gcacaacatc ggctccatca tatgcttcag ccgtcttcgt tccttttttg attttacatg
      481 ggtataatcc aaggcgtaaa gttccaccca aattctcgat gttttcctga tcagccatta
      541 aatcaataat attgtttgct gtatctggat tcgtttcagc tgatgacgca tcttctaatc
      601 caacgacgtt acgtgcaaat tcgacacaag ccatctgcat accaagacaa atacctaaaa
      661 acggaacgtc attttcgcga gcataacgaa tcgcttcgat tttcccttct actccgcgat
      721 caccaaatcc accaggaacc aagattccat ctgcgtcttt caaacgttct tctacatttt
      781 ctctcgttaa ttcttgtgct ttgatccaat cgatctcgat gtctgaatta taggcgaaac
      841 cagaatgttt caatgcttca actactgaga tatatgcatc tggtaactcg acatatttac
      901 cgacaagcgc aatccgtact tttttcttca agttcaagac acgttcttct aattctttcc
      961 attctgtcat gtctgcttct ggtgtatcta gttttaaatg gtcgcaaaca atttgatcca
     1021 ttccttgtgc ttgtaaaact aatgggatgg agtaaagtgt atctacatct ggcgactcga
     1081 taactgcttc tggttcgaca tcacaaaatt gtgccaattt gtttttcacg ctttgagaaa
     1141 caggttgctc tgtacgtacc accaaaatat tcggttggat acctaaacca cgtagctctt
     1201 ttacactgtg ctgcgttggt ttagttttca tttctccagc agctttcaag taaggaatca
     1261 atgttgtatg gatatacata acattatctg taccgacatc tgctttcatt tgacgaagtg
     1321 cttcaaggaa tggcaaagat tctatatccc ctactgttcc ccctacttct gtaatgatca
     1381 catctgaatc agtcatttta gctgctcgca tgattttttc tttgatctca tttgtaatat
     1441 gaggaataac ttgaacagtt gctcccaagt attcgccctt acgttcttta cgcaaaactt
     1501 cagaatagat ttttcctgtc gtcacgttgg agtatttgtt caaattgatg tcaataaaac
     1561 gttcgtagtg gcccagatcc aaatctgttt cagcaccatc atctgttacg aatacttctc
     1621 cgtgctgata tgggctcatc gtccccggat ccacgttgat gtatggatca aatttttgga
     1681 tagttacttt taagccgcgg tttttcaata agcgtcctaa agaagctgca acaatccctt
     1741 ttccgattga tgaaacgaca ccgccggtca caaaaatata ttttgtcata agttcaaaaa
     1801 ctccttttta atttatgtgc aggaaaagtc tcagacaata aacaaagctc cctattcttc
     1861 aataagaata gggagccgaa atttctgtaa acttctgacc ctctaaaagg gtgcccaagg
     1921 tgtataatac aagctgacta agggaaagtc aagtactcaa atgaatacta tccgcttaca
     1981 attttttctt tttcgatatc ctttgagaaa ttatttctta agtcctttat gagtgcaaaa
     2041 aaaatttaac ccacataata agcttgatat aaacatagct aacgttaaac ctaagcaaat
     2101 cagaaaatcg ttactgaaaa attttaagtg aaataataga taaaaaaata aaacaaacat
     2161 tatgatagat aatatcccac ctaacaaaga actacttttt cctacttgca gcaacagttt
     2221 atatagctta tcgtagaaac aatctttttc tttcaccctt atttacctcc tttacgtaaa
     2281 aaacgtagtc cacagttctc ggatttttgg catatctttt ctattaaata atatcaaaaa
     2341 caaaacaatt ccactaattt tttcaaagat gacctttttt tcaacaaaag tgccttgtag
     2401 ggattcattg aaggtaacga atcacataag taaaaaataa ggagatgcga caaaagtcgt
     2461 gcctccttat ttctaaataa acggtgttct cctgccaact cctcggtcac aagtcacaat
     2521 atccatcaaa catgagaagc tgtttttgaa attatttctt gtgattcgga gttaaatggt
     2581 aggctcacaa cctctggata aacggtattc aaaagccagt ttctcggtat taagccaaat
     2641 ttatgcaaaa atgtagaaag ccattttcac aaaatttttc ttaatactcg aaactaaacg
     2701 tctttttcaa aacctcttgg taaacggtgt tcaaaagctt gcatctgcgg taataagttc
     2761 agccaattca aaaacaagaa aagctgtttt tgcttggcta ttcttattac ttgttgcagg
     2821 gcagcttttt cacaacctct ccactaaacg gtgttcaatc agctgaccct cggtattagc
     2881 tcaaaaaacg gcaaaatatg aggaactatt tttccgttag tttctctaat actcaggtca
     2941 aaacgctgat ttcacagcct ctaattcacg gtattcctgt tttaataaag ttctagatat
     3001 tgttctcttt cccactcgga aacagtttgt ctgaatgccg cccattcgag acgttttgct
     3061 tcaacaaaat ttgagaagat atgttgtcct aatgcgtcaa tcattacatt atccttacgc
     3121 aattctttga tagcattatg gatagttgaa ggtaagtcct ggatatgtgc agcttctctt
     3181 tcttcctcat ccattacata aatgttacgg tcaacagctg ctggcggtgt gatctgatta
     3241 cggataccgt ctagtcctgc ttgcaacaat actgccattg ccaagtatgg gtttgcagat
     3301 ggatcaactg accgcaattc caaacgtgtg gataaaccgc gagattctgg tacacgtact
     3361 aatggcgaac gattacggcc actccatgcg acataaacag gcgcttcata acctggaacg
     3421 agacgtttat aagaattgac tgttggattg caaacggctg tgtaggcacg agcatgtttc
     3481 agcaaaccac ctaagaaatg ataagctgtt tcacttagtt ccaattcacc gtctttatca
     3541 tagaaaacat ttccttcatc attgaacaaa gacatattgc aatgcatacc agaaccattg
     3601 atcccaaata acggtttcgg catgaaagtc gcgtgaagtc catgcttgcg tgcaatcgtt
     3661 ttgacaacga gcttgaaggt ttggatatta tcacaagcat ctaccacatc tgcatattta
     3721 aagtcaattt catgctgtcc tggcgctact tcatgatgtg aagcttccac ttcaaagcct
     3781 agactttcta attccaacac gatgtcacgg cggcaatttt cacctaaatc tgttggtgca
     3841 aaatcaaaat aaccaccttt atcattcaag gtcgttgtga tcccaccgtc ttcgtctaat
     3901 ttaaataaga agaattctgg ttcaggtcct aaattgaaag aggtaaaacc aaggtcgcgc
     3961 atatctttca atgctcgttt caaattcccg cgaggatcgc cggcaaatgg tgtcccatcc
     4021 ggattataga tatcacaaat taaacgagca acttttccat gagcactttc ccacgggaag
     4081 atcatccaag tggagagatc tggatacaaa tacatatcgc tttcttcaat tcgaacaaat
     4141 ccttcaatcg aagaaccgtc aaacatcatt ttattttcta atactttgtc gatctggctc
     4201 actggaactt ctacattttt gatcgtacct aagatgtctg tgaacatcaa tcgtaaaaat
     4261 cgtacgtttt cttcctcaat gattcgtttg atttcttcac ctgttatctg tttctttacc
     4321 atggtatccc tcttcctttt ttaatttatt cagtcaaaga attgtctttg caatccttgc
     4381 gactgataat gacctgtttt gcttaaccca ccttgcgcca aaatctcatc acgaaaaatt
     4441 tgtcgaacgt catgatctgt caacggcttc ttgttttttg cttcttgttt tttcgcctgt
     4501 gccatttcgt atacacgttt gatcccagcc atattcaatc cctctgaaag atagtcttta
     4561 atctcaagca acatatcaat atcattcagc gaatacatac gtcggtttcc ttcgcttcgt
     4621 tccggatgaa ctaaatcttg ttcttcataa tagcgaattt gccgagcaga caaatcagtc
     4681 agcttcataa ctgtaccaat tggaaatacg gacatagatc ggcgcaattc cttttctccc
     4741 atcagacgtc ccctttcgtt ttataagttg gctaaagtgt accaattaaa aaaaatattg
     4801 tcaactgcat atgttaggtt ttctaacatg agatctctat tttttataaa ataacaaaaa
     4861 aataagaaca caaaaaaggt atctagttga ttttcgtac
//
LOCUS       DAKBVG010000176         4861 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000176 DAKBVG010000000
VERSION     DAKBVG010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4861
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.176"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            222..1556
                     /gene="gmhB"
                     /locus_tag="OUD09_002231"
     CDS             222..1556
                     /gene="gmhB"
                     /locus_tag="OUD09_002231"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HCT9693466.1"
                     /translation="MKTVIMAGGRGSRISELFPDIPKPMIPIDGIPVLERELCSLRDQ
                     GFNDIILTVSYLHEKIEEHFGDGSKLGIKIKYFIEKTPLGNAGALYFLLDELGDEPFL
                     LLNADAVFDVDFRQMIRFHVENNADVTLFTHPNSHPYDSGLIISDDNKKVLQWLAKED
                     QRPEYYKNRVNAGLHVINPKVLRENPLMEIVNKSIVEGGGLPYKIDLDRMILKPLANT
                     GRMYCYDSPEYVKDMGTPDRYRAVIKDFQKGIIVGKNLSNPQKAIFLDRDGTINKYVG
                     FLRNAEEFELIDGVSEAIKIINSLGFLCIVITNQPVIARGEVTVDELNSIHNKLETML
                     GHAGGFLNGIYYCPHHPHKGYAGEVIELKIDCNCRKPKPGLLLEASKDFNINLSQSFM
                     VGDSESDVIAGKNAGCKTVLITEDITLPANYGQDYSVCSLLEFVKNHLCTMQ"
     gene            1591..3228
                     /locus_tag="OUD09_002232"
     CDS             1591..3228
                     /locus_tag="OUD09_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HCT9693467.1"
                     /translation="MKVLIPAAKVVEKELQSIGMLPAIIYPINKKIVFDYLYNQYNAI
                     CDSLDIICYEKADKVHRRLKNYHDSRIHICDLPELGDLGQTVYFGLSDEDEPVLINFA
                     DTIVMESMSDIEMDTFYYSEDFVSSTWTYFKINEGIITDIHDKEEAYVSDKREKLFIG
                     VFMFSSSKLLKSCLREAVESRKQGGINSFYRALQLYSERCPLKAVRTEKWFDIGHIDK
                     YYNTKLEVKAREFNHISIDKDRGILKKTSDDKDKFIGEIKWYLKLPADIEYVRPRIFD
                     YSTSYVAPYVDMEYYAYHTVHELYLYGDLKYNQWHDLFSRIKFICSDFKRYTLKDEKI
                     RESLEDMYLRKTMQRFQKLSEDKRFSAFFEKQIQINGRKFISLTGVIELLKNLIPSML
                     YDVNEFCIIHGDLCFANMMVDSNCSFVKVIDPRGKFGKYDIYGDSRYELAKLFHSLDG
                     KYDYIIKDLFTVEYDTECAVIDYTINTRVRDYDLFELFKSVFSDEIKDDFDKIVLIEA
                     LLFLSMIPLHGESFDHQIVMLATGMELLARVSNKVIL"
     gene            3330..3725
                     /locus_tag="OUD09_002233"
     CDS             3330..3725
                     /locus_tag="OUD09_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HCT9693468.1"
                     /translation="MYSEYSFVFDIDGTLCPIKKKDERYEDLVPYENMVEKLRFYKEN
                     GAKITLFTSRNVNSYKGNLGMINANTAKVLLAWLDKWDIPYDEILYGKPWPGHKGFYV
                     DDRTVRPDEFLRCSVDELNEICNSSKKRF"
     gene            3743..4474
                     /locus_tag="OUD09_002234"
     CDS             3743..4474
                     /locus_tag="OUD09_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCT9693469.1"
                     /translation="MEKKMTVIITMAGLGSRFRKIGYAVPKYMINAKGRTLFDWAMDS
                     LVGYNKNVDKYIFVVRQEDNASAFIKEHCAYFGIENVEVIEIDYMTDGQATTCMLAIP
                     YCNQDNEIMVYNIDTYVESNEMKYEDICGDGHIPCFHADGDHWSFAKLNDRNEVIEVR
                     EKVRISDNCTLGAYYFSSAKLYKALYEEYYADSSNMEKNEKYIAPLYNFMIKKGLKVT
                     ISIVDAKKVHVLGTPEELNVFLNQE"
     gene            4498..>4861
                     /locus_tag="OUD09_002235"
     CDS             4498..>4861
                     /locus_tag="OUD09_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HCT9693470.1"
                     /translation="MTSNNLIVKTLSRYKNTPLPVKAALWFMVCGFLQRGVSMITTPI
                     FTRVLSTEQYGLYSTYLSWESLLLMITSLSLFKAMMNLYVKYDNQEHILSTVSGLTLV
                     LTIFWFVIAIFIKDILAEI"
BASE COUNT         1654 a          606 c         1012 g         1589 t
ORIGIN      
        1 cataaatatg taggtcgtta ttcaaataat actatttatg gtacgtatta tattgatgat
       61 agccaagatg cacttcactg ctataatttt gattttataa attggttgaa tttgtacact
      121 ggaaaatacg aatacaaagc agaatatgaa aaatatatgg actttgtttg ggaagagtaa
      181 ttaactttta tagtaaaaag tactttaggg aagggcatat tatgaaaaca gtaattatgg
      241 cgggcggacg aggaagccga atttcggaat tgtttccgga tattcctaag ccaatgattc
      301 ctatagatgg cattccagtt ttagaacgag aattgtgttc gcttagagat caaggcttta
      361 atgatattat tttgactgtt tcctatttgc atgaaaaaat agaggagcat ttcggagatg
      421 gttcaaaatt aggaattaaa atcaaatatt ttattgaaaa aactccactt ggcaatgcag
      481 gtgcattgta ttttttgttg gatgaactgg gtgatgaacc gtttttactt ttgaatgctg
      541 atgcagtatt tgatgttgat ttcaggcaga tgattagatt tcatgttgag aataacgcag
      601 atgttacttt gtttacgcat cctaactcac atccgtatga tagtggatta attatatccg
      661 atgacaataa gaaagtgctt caatggttag cgaaggaaga tcaaagacct gaatattata
      721 aaaaccgagt aaatgcaggt cttcatgtta taaatccgaa ggtgttgaga gaaaatccat
      781 taatggaaat tgtaaataaa tcaatagttg aaggtggggg gctaccatac aaaattgatt
      841 tagatagaat gattctgaaa ccattggcta acacagggcg tatgtattgc tacgattctc
      901 cagagtatgt taaagatatg ggaacaccgg accgatatag agctgtaatt aaggactttc
      961 aaaagggaat tattgttggt aaaaatctat ccaatccaca aaaagcaatc tttttagata
     1021 gagatggaac gattaataaa tacgttggtt ttttgagaaa tgcagaagaa tttgagttaa
     1081 ttgatggagt atcggaggca attaaaatta ttaattctct aggttttctg tgtattgtta
     1141 taacaaatca gcctgtaatt gcaagaggtg aagttactgt tgatgagctt aatagtattc
     1201 acaataagct tgaaacaatg ctagggcatg ctggtggatt tttaaacggt atttattact
     1261 gtccgcacca tccccataaa ggatatgcag gagaggttat agaattaaaa attgattgca
     1321 attgtcgcaa gccaaaacct ggactgttat tagaagcatc aaaggatttt aacataaacc
     1381 taagtcaatc atttatggtt ggagatagtg aaagtgatgt aattgctgga aaaaatgctg
     1441 gttgtaagac agtactgatt acagaagaca tcaccttgcc tgctaattat ggccaagact
     1501 atagtgtctg ttcattgtta gaatttgtta aaaatcattt atgcactatg caataattgg
     1561 tttagtgaaa cttgtaaaag gagtaattag atgaaggtat taattccagc agcaaaagtt
     1621 gttgaaaaag aattgcagag tataggaatg ttaccagcaa ttatctatcc aattaataaa
     1681 aaaattgtct ttgattattt gtataatcag tataatgcaa tctgcgattc attggatatt
     1741 atctgttatg aaaaagcgga taaggttcat agaagactga aaaactatca tgattccaga
     1801 attcatatat gtgaccttcc tgaattaggg gatctagggc aaaccgtgta ttttgggtta
     1861 tcagatgaag atgagcctgt tctaattaac ttcgcagata caattgttat ggagagtatg
     1921 tctgatatag aaatggatac attctattat tctgaagatt ttgtgtcgag tacttggaca
     1981 tactttaaaa ttaatgaagg aataattact gatatccacg ataaagagga agcttacgta
     2041 tccgataaac gtgaaaaatt atttattggc gtatttatgt tttcttcgtc aaaacttctg
     2101 aagagttgct taagagaggc agttgagagt cggaaacagg ggggaataaa ctcattttat
     2161 cgggctttac agctatacag cgaacgttgt ccgctaaagg ccgtaagaac agaaaagtgg
     2221 tttgatattg gacacatcga taaatattac aatacaaagt tggaagttaa agcacgagag
     2281 tttaatcata tctctataga taaagatcga gggatattga agaaaactag cgatgacaag
     2341 gataaattta ttggagaaat aaagtggtat ttaaaacttc ctgctgatat tgaatatgta
     2401 agacctagaa tttttgatta ttctacatct tacgtagctc cctatgttga tatggaatat
     2461 tatgcatatc atacggttca tgagttatac ttatatggtg atttaaaata taaccagtgg
     2521 catgatctgt ttagccgtat aaagtttatc tgtagcgatt ttaagcgata caccttaaaa
     2581 gatgaaaaga ttagagagtc attggaagat atgtatttgc gaaaaaccat gcagagattt
     2641 caaaaattgt ctgaagataa aagattttca gctttttttg agaaacaaat tcaaattaat
     2701 ggtcgtaagt ttatttcttt aaccggtgta attgaacttc taaagaatct tatcccaagt
     2761 atgctctatg atgtaaatga attttgtatt atacatggag atttgtgttt tgctaatatg
     2821 atggtagatt ccaattgttc ctttgttaag gttattgatc ctaggggaaa atttggaaaa
     2881 tatgatattt atggggacag cagatatgaa ttagcaaaac tatttcatag tttagatggt
     2941 aaatatgatt atatcatcaa ggatctattt actgtagaat atgataccga gtgtgcagtg
     3001 atagattata caattaatac gcgtgtaaga gattacgatt tatttgaatt gtttaagtcg
     3061 gtcttttcgg acgagattaa ggacgatttt gataagattg tattgataga agcgttgcta
     3121 tttcttagta tgattccatt acacggtgaa agttttgatc accaaatagt gatgcttgct
     3181 acaggtatgg aacttcttgc aagagtaagt aataaagtta tactctaata taaattgtgc
     3241 cttaatgaat aacggttaca atactgtttt agcaatgtag gcattaaggc acgattatat
     3301 taattaatac aatagaaggg aagagaaaaa tgtatagtga atattctttt gtgtttgata
     3361 tcgatggcac tttgtgtccg ataaagaaga aggatgagcg atatgaggat ttggtgccgt
     3421 atgagaatat ggttgaaaaa ctgagattct ataaggagaa tggtgctaaa attacattgt
     3481 tcacctcacg caatgttaat tcctataaag gaaatttggg aatgataaat gctaatactg
     3541 caaaagtgct cttggcgtgg ttggataaat gggacattcc gtatgatgaa atcctgtatg
     3601 gtaagccttg gccggggcat aaaggcttct atgtagatga tcgaacggtt agacctgatg
     3661 agtttttgag atgctctgta gatgaattaa atgaaatctg caacagttca aaaaaaagat
     3721 tctaaataag gaggttagtt ctatggaaaa aaagatgaca gttatcatta cgatggcagg
     3781 tttgggaagt cgttttcgaa aaattggtta tgcggttcct aaatatatga ttaatgccaa
     3841 aggaagaact ttatttgatt gggccatgga tagtttggtt ggatacaata aaaatgttga
     3901 taagtatatt tttgttgttc gtcaggaaga caacgcttca gcttttataa aggagcattg
     3961 tgcttacttt ggtattgaaa acgtagaagt gattgaaata gactatatga cagatggtca
     4021 agcaactaca tgtatgttgg ctattcctta ttgcaatcaa gacaatgaga ttatggttta
     4081 caatattgat acatacgtgg aatcaaatga gatgaaatac gaagatattt gtggagatgg
     4141 acatattcca tgctttcatg ctgatggcga tcattggagt tttgcaaagc ttaacgatcg
     4201 aaatgaagta atagaagtcc gcgaaaaagt tcgcatttct gataattgta ctcttggtgc
     4261 gtattatttc tcatcggcaa agctgtataa agctttatat gaagagtatt atgcagactc
     4321 ctctaatatg gagaagaatg aaaaatatat agcaccatta tataacttta tgataaaaaa
     4381 agggttaaag gtgactatta gcatagtaga tgcgaaaaag gttcatgtgc ttggtactcc
     4441 agaagagctc aatgtgtttt tgaatcaaga ataaatagtg tttaggagaa aatagcagtg
     4501 accagtaata atctaattgt aaaaacatta agcagatata agaatactcc acttcctgtt
     4561 aaggctgcat tatggtttat ggtatgtggt tttttacaga gaggggtttc aatgattaca
     4621 accccaattt ttactagagt tttgtctaca gaacaatacg gtttatacag cacttatcta
     4681 tcgtgggaat ccttattgct catgattaca tcgcttagtt tgttcaaagc catgatgaat
     4741 ctttatgtaa aatatgataa tcaagaacat attctttcga cagtatccgg attgacttta
     4801 gtactgacta ttttttggtt tgttattgct atttttatta aggatatatt ggcagaaatt
     4861 t
//
LOCUS       DAKBVG010000177         4805 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000177 DAKBVG010000000
VERSION     DAKBVG010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.177"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            180..455
                     /gene="rpsP"
                     /locus_tag="OUD09_002236"
     CDS             180..455
                     /gene="rpsP"
                     /locus_tag="OUD09_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCT9693471.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            467..718
                     /locus_tag="OUD09_002237"
     CDS             467..718
                     /locus_tag="OUD09_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HCT9693472.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            843..1634
                     /locus_tag="OUD09_002238"
     CDS             843..1634
                     /locus_tag="OUD09_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693473.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALRMAVSMKRL"
     gene            1804..2325
                     /gene="rimM"
                     /locus_tag="OUD09_002239"
     CDS             1804..2325
                     /gene="rimM"
                     /locus_tag="OUD09_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCT9693474.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVGLTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            2315..3076
                     /gene="trmD"
                     /locus_tag="OUD09_002240"
     CDS             2315..3076
                     /gene="trmD"
                     /locus_tag="OUD09_002240"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCT9693475.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            3212..3559
                     /gene="rplS"
                     /locus_tag="OUD09_002241"
     CDS             3212..3559
                     /gene="rplS"
                     /locus_tag="OUD09_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCT9693476.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            3817..4110
                     /locus_tag="OUD09_002242"
     CDS             3817..4110
                     /locus_tag="OUD09_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HCT9693477.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT         1704 a          822 c          937 g         1342 t
ORIGIN      
        1 actgacaaaa gctacataga agatagatta cttattttta acaaaaaaga agtctaaaat
       61 cttctatttc ctaaaaaaaa tcgaatttaa tcaagctgta aagaaaaaaa gctttacagg
      121 gtaaaaaaaa actgttacac tacatcttgt gaataaaaca atggaggtgg aaataaataa
      181 tggcagttaa aatccgttta aaacgtatgg gttctaaaaa gagtccgttt taccgtatcg
      241 tcgtagctga ttcacgttct cctcgtgatg gacgtttcat cgaaactgta ggtacttaca
      301 atcctttgaa agaccctgca gaagtagttt taaaagaaga cttagttctt gactggttat
      361 caaaaggtgc acaaccttct gacactgtac gtaacatcct ttcaaaagaa ggcgttatgc
      421 aaaaacacca cgaagctaaa ttctcaaaga aataaggtga ccggatatga aagatttaag
      481 cgacttagtc ttaacaatcg ttcgtccgct agtcacttat cctgatcaag tcaagttaga
      541 cgtcgtagag tcgaaagact tttacgagta caatctaaca gtggctcctg aagatattgg
      601 ccgtatcatt ggaaaacaag ggcgtgttgc taaagcaatc cgcacaatcg tttacggtgt
      661 tcggatcaat gcaccgaaaa aagtgcgttt gaatatcatc gatgataagg aaaagtaaat
      721 aaacgtgctg tactcgaatt ttcgagtgca gcttttttgt tttttttatt aattgaagaa
      781 atgattattt ggcaaaacaa gcaagaaagc gtatccttat aggaggaggg agtgaacagc
      841 caatgagaaa gaaaatgtta tttattccat ttgctctatt gttaagcgct tgtagttcgg
      901 ttactcaaga atcagacaat acacatctat caagcagtat atctgaaact gttaccagtc
      961 cagaaaaaag tagtaccaaa acggcttcca ccactgaaca aacaatggct acctcaaata
     1021 atgagaaaaa gactgcttta gaccagttga aagaacaaca gccaaatgtt cccatgcctc
     1081 tagatgtacc agtctcttca ggctatctca atatcgcagc gacacataca aagcaaggtt
     1141 attctatttt atattacaga acagatcgtc ccctcgggtt gaatgctgac gaattgaatc
     1201 aagaaacgcc tattgcaacc tatctatatc aatatgggtt tgcttctagt caagaaacaa
     1261 tccaagtttt gcagccattt gagatcgaca caaatggtca gcaagtggac ttaggttctc
     1321 gaatcacagg gtatcagcaa ggtgcagcag gttcaagttt tcttgaatgg caagaaggaa
     1381 actggcgtat tcgaatccgt gggaataata tagaaggcca agatccctta ctattagcta
     1441 aagaaatcgt agcttatttg gaagaaaaca gtctaccagc acctgaacaa tttggtaaaa
     1501 ttactgttga tatgggtgat acgacgaatc gagccgtaga agtaagctgg caagaaccga
     1561 aaaatgccta tacgatcact catcaagatc ctatgtctgc gttgaggatg gcagtttcta
     1621 tgaagaggtt gtgaaatcag cgcgctgatc tgagcccatc atttaactcc aagtcacagg
     1681 aagtaatcaa aaatagcacg ccgcattttt gcataatttt ggcttaatac cttaaaagaa
     1741 aactagaatt gcctcttcac acatgctaaa ataaaacaga caaaaaagaa atggggatat
     1801 aagttgactg aatacttgaa tgtaggaaaa atcgtaaaca cacaaggtat caaaggcgaa
     1861 gtccgtgtta tctcaacaac ggatttccca gaagaacgtt acaaaaaagg aacggtgcta
     1921 acactattcc aagacggaaa agcacctgtg ggattaacag tcaaaagtca tcgcaaacat
     1981 aaaaattttg acttattgag ttttgaagga catccttcta tcaatgatgt ggaaaaatat
     2041 cgggatggca tattaaaagt gtcaaaagac aactctgtag atctagctga aaatgaattc
     2101 tattaccatg agatcatcgg tctgcatgta ttagaagatg gaactgagtt aggtaaagta
     2161 aaagaaatct tgtcaccagg tgccaatgat gtttgggtcg ttcaacgtcc gaaaaaagct
     2221 gatatattgc ttccatatat cgcttctgtc gtcaaagaag tcgatttaga agctggagtg
     2281 gtccgtgtag aaattccaga aggactgatt gacgatgaag attgatgtat tgactttatt
     2341 tccaagaatg tttgaaggtc ctatggggga atccatcatc ggtaaggctg tagataaagg
     2401 attattagat atcaatatct ctaacttccg cgattattca gataacaaac atcagacagt
     2461 agatgattat ccatacggcg gtggagcagg tatgctgctc aaagtccagc ccatctatga
     2521 taacatccaa gccattgaaa aagctgcacc ggatgtcaaa aaaagagtaa ttcttcttga
     2581 cccagctggt aaacgattcg atcagaaaat ggcagaagaa ttttcacaag aagaacactt
     2641 agtattcatt tgtggtcatt atgagggata tgatgaacgt atccgcacat tagtgacaga
     2701 tgaagtttcc ttaggagatt atgtccttac tgggggagaa ttaggtgcga tggtcatgat
     2761 cgatgcaaca gttcggcttt tgccagatgt gttagggaat cagacttcag ctcaaacgga
     2821 ttcttattca accggtttat tggaacatcc ccaatatact cgcccggcag aatataaagg
     2881 gatgaaagta ccagaagtat tgacaaatgg aaatcataaa ttgattgaag aatggcaatt
     2941 aaaagaatca ctaagaagga cctatcaacg tcggcctgat cttttggaaa cacttgaatg
     3001 gacacctcaa atgacgaaat ttttagaaga aatcaagaaa gaagaacaag aaaaaacagc
     3061 agatgaagcc aagtgacttt ttctaaaatc ttctttacag taggttgata atatgttagt
     3121 attttatggt gagtgcaaaa gcacttaact attacgatat tccgctgtga gaaaagctca
     3181 taagaatatt tggaataagg agaagaaaac catgaatcca ttaatcgaag aattaacaaa
     3241 agaacaatta cgttctgaca tcccagcgtt ccgccctggt gacactgtgc gtgttcacgc
     3301 aaaagttgtt gaaggtactc gtgaacgtat ccagttattt gaaggtgttg ttatcaaacg
     3361 ccgcggtgct ggaatcagcg aaacttatac agtacgtaaa gtttctaacg gagtgggcgt
     3421 tgaacgtaca ttcccattgc atacaccacg tgttgcaaaa atcgaagtag ttcgctacgg
     3481 taaagtacgt cgtgcaaaat tgtactacct acgtgcatta cacggaaaag ctgctcgtat
     3541 caaagaaatc cgtcgttaat cattgttatc agactgagaa agaacccttg gtattcaagg
     3601 gttctttttt cttagcatgt aatttgaaca ccctgatcca gttttttctt taattattgg
     3661 taggggagaa taatggatta gaataaatct acctataagt agtagtaaaa aaaagagcac
     3721 tatgataata tctttcttaa gagtaaccgc tttaataaaa aatccagaat attctaacat
     3781 aaatggagta ttcttagaat caaaaggaga aagaagatgg tttatactta tacggagcaa
     3841 gaaaagatga aagttttaga cacattcatg aatgatggaa aagtaaaaca agtaccaagt
     3901 tcaacaaaaa agaaatacat cctattgaaa gaagtcttga aacgctttga ccatggagtg
     3961 acttatacag aaacagaggt taattcgata ctgctgaacg tattttctag tggcgattat
     4021 gtcgaacaaa gacgttatct aatcacattt ggctttttca aaagaagttc agacggtcgg
     4081 gcatatcaga tgatgggaat tgaaaattag aagttttaat ttatactcat tgaaaaaagt
     4141 tcaagtatgc gatattggtt ctacaccgca tacttgaact ttttttgtca tctgtatcta
     4201 taatacaaag ccaagaacgt tattcaaaat atggatagct atgataaaag ctaatgagcg
     4261 attcgcagtt aaataagcaa tcgtcagtaa aatcgttcca ggagcgtaca tgaaaaaatc
     4321 aattggatgc gttacatgaa taaagataat tctcttttat ttgtacgaat aaataccaaa
     4381 acgtacctcc taaaattaga ttttggacag atgcccttta aaaagataac aagatttcgt
     4441 tcttgaaaca atatatatgc ttttattcca atgaggttgt gaaaacagta ttttgtcctg
     4501 agcgttagaa aattgaaaat ggggaatagc aactcgtact ttaccatttt ttaagtgaat
     4561 acaagatcag ctaattgaac accgtttatt cgtgatgagg gacacaacac tgcagtcgac
     4621 acatttctct tttatgttca aagataacaa atatttaaaa caaagaaaaa tagtagataa
     4681 tcccgtccta actcttttat tcttccctca tagcttccaa atttaagcaa tacttcaatt
     4741 aatcaagttt tatctttttt tttcaataat cgtaaaaaaa gagtattttt tatttaaaaa
     4801 taaaa
//
LOCUS       DAKBVG010000178         4767 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000178 DAKBVG010000000
VERSION     DAKBVG010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4767
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.178"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            <1..354
                     /locus_tag="OUD09_002243"
     CDS             <1..354
                     /locus_tag="OUD09_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replisome organizer"
                     /protein_id="HCT9693478.1"
                     /translation="PSKKSKAKPVRHKYGEYKNVLLSDEQMEKLKTEFPNDYQERIER
                     LSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGRREKLPEWAIDQEAYLK
                     KKALERANRQSKAPF"
     gene            366..635
                     /locus_tag="OUD09_002244"
     CDS             366..635
                     /locus_tag="OUD09_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693479.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            640..804
                     /locus_tag="OUD09_002245"
     CDS             640..804
                     /locus_tag="OUD09_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693480.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            797..1099
                     /locus_tag="OUD09_002246"
     CDS             797..1099
                     /locus_tag="OUD09_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693481.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            1096..1257
                     /locus_tag="OUD09_002247"
     CDS             1096..1257
                     /locus_tag="OUD09_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HCT9693482.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            1254..1559
                     /locus_tag="OUD09_002248"
     CDS             1254..1559
                     /locus_tag="OUD09_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693483.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            1559..1915
                     /locus_tag="OUD09_002249"
     CDS             1559..1915
                     /locus_tag="OUD09_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HCT9693484.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            1902..2120
                     /locus_tag="OUD09_002250"
     CDS             1902..2120
                     /locus_tag="OUD09_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693485.1"
                     /translation="MDLYDIESGATLSVESDHLIINFGGKRQIILWVVDDVLFPEIVH
                     DFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            2117..2536
                     /locus_tag="OUD09_002251"
     CDS             2117..2536
                     /locus_tag="OUD09_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HCT9693486.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            2533..3090
                     /locus_tag="OUD09_002252"
     CDS             2533..3090
                     /locus_tag="OUD09_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HCT9693487.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            3087..3383
                     /locus_tag="OUD09_002253"
     CDS             3087..3383
                     /locus_tag="OUD09_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693488.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            3460..3873
                     /locus_tag="OUD09_002254"
     CDS             3460..3873
                     /locus_tag="OUD09_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HCT9693489.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIETERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            4182..4334
                     /locus_tag="OUD09_002255"
     CDS             4182..4334
                     /locus_tag="OUD09_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693490.1"
                     /translation="MKLSVEGTPEEIQELLQAIGSSKEQKVSVSNSDIDGICNCLNRE
                     KISNPY"
     gene            complement(4331..4606)
                     /locus_tag="OUD09_002256"
     CDS             complement(4331..4606)
                     /locus_tag="OUD09_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693491.1"
                     /translation="MYKPYMISYDLNNPGQKYDKVFEIIKEFGAYIKLQKSFWLVKTN
                     LNPNQMCEKLNTVLDNNDSLFICELQKNYQGRATEENWKFINEHIFS"
BASE COUNT         1729 a          739 c         1065 g         1234 t
ORIGIN      
        1 ccttcgaaaa aatcgaaggc taagcccgtc cgtcataaat acggagagta taaaaatgtt
       61 cttttgtcag atgagcagat ggagaaactc aaaacagaat tccctaatga ttaccaagag
      121 cgaatcgaac gactgtcaga gtattgtgaa tcatctggta agacttataa aaactatttg
      181 gcaactattc gaagttgggc aaggaaagaa aaaagtgaac ctaagaacgc aagcagtgga
      241 tacaagcgca caggaagacg agagaagctt cctgaatggg caatcgacca agaagcctat
      301 ctcaagaaaa aagcgctaga acgagctaat agacaatcaa aagcaccatt ctaagaggtg
      361 gaaaaatgaa gatcgattat ctagaactaa ttaatgaaat agcgaattat aaaaagggcg
      421 aggaattaga cgtcctgaga gacgtatatg atcaactcga agaagctgga attgaaggaa
      481 ttaagaatga tcgttcgagt tggagtaagc tcagatacta tttcgcactt tatatcgata
      541 caacacaatt aagaaattta gcatatacca aattactatt tgttgattgt gttaaaggct
      601 tgcaaaaaca tcttaatgaa cttgagcagg tgtaatcaga tggacctaaa gacatttaca
      661 gcacagattg aactaatgca tcaagaagct ttaagacaaa gtgtatcgta cgaagacaag
      721 tggctcaaca cgttccatgg cggacgtgag agcgcacttg atcaagtact caaattactg
      781 aaaggagaat gtcgggatgg ataagaaagc ggcaatgcag cgaattatcg aattgactta
      841 ttcagaagat tggcaaaatg acaaagaagc tgcttcagaa gtgatgaggc ttggaagagc
      901 gatgtgggca gacaagagca acaagccaag accacgaaaa atcgcaattt ggcacggtga
      961 caaacttcta gtgataggga cagctgaaca gttagcaagt ctcacaggct tgcacgagaa
     1021 aatcgtgagg aaaagagcaa ggtgtggcta cacagacgct aagaagagaa cgtttagata
     1081 cgtggaggaa tcatcatgac aacagaagaa gtgattcaaa tgcgtattcg aagccttcag
     1141 cgtgagattg acgatctgga acgaacaaag gcagtgatgg tcaatgaaac ggcgagaaag
     1201 gcaatcgatt tgcacataga gaatttaaga agggaaatcc atcgattgga ggaatgagcg
     1261 tggataagaa agcagcaatg aaaagaattg ctgaattaac caagtcagaa tcttggcaag
     1321 aagacaaaga aatagttgca gaagtccaaa agctcggtaa atcaatgtgg actgaaaagc
     1381 ccaaacggag aacgccgaga aaaattgcaa tctggcatga tgaccgaatt ttagtaacag
     1441 gtactgctga acagttatct gaaattactg gattaagcaa aaacattatc tgggatagag
     1501 ctaggagctt atggattgat tcaaaaggac gacagtttag gtatgtggag gagagataat
     1561 ggatctcatt acacaataca gtgacatcat cctcaagaaa atcatgatga agattcagaa
     1621 agataaaaaa tcaaaagaac gagctgaatt agttaagtta gaaatggctg aaacaggagc
     1681 aggagtgcga agtagcaggc attggaaagc agcagcaaac attgaatttt attacaacga
     1741 aattcaaaaa gggttcgatc agatgcgtga gctggatcgg caaacaaatt ggagcaagaa
     1801 acttcatcaa gatcgtttca aatttgtaga gaagtataaa gaaatattag aagagtattt
     1861 gaggaggaca gcaaatgata aaaaaactcg ttcaattcag catggattta tatgatatcg
     1921 aatcaggagc aacactatct gtggaatcgg accatctaat cataaatttt ggtggaaagc
     1981 gccagattat tttgtgggta gttgatgatg tactgtttcc agaaattgtt catgatttcg
     2041 aagaatcaaa agcggttgag tttgaaatag tgaaaaaagt aatggaattg attgaaaaat
     2101 acgaggagga cagcgaatga taccgaagtt tagagcgtgg gataaacgaa agaacgtaat
     2161 gagagatgta gccgtcttgc attttactaa aaacggcaaa acaaacttta ttgaatattg
     2221 gataaatcct accgaattga aatcatatca cgtgcgaaac atcgacctca tgcaatccac
     2281 aggaatgaaa gataagaatg gtgtggatat atttgaaggg gatatagtat tagtcagcgt
     2341 gcgaaatggc ttcgattact tagataataa agtctgtatt gtcaaaaatt caatagatta
     2401 ttccggatta gtttgtgcca ctgttgatga agacttagag tatcaaattt ttaacacaga
     2461 gctgtttgaa gaatacacgt acgaagtcac cggaaatata tacgagaata gcgagttatt
     2521 ggaggaacag cgatgaataa acaggaagca atcaaaaagt tagaaagtat taaagcgata
     2581 ggaaatgatg caatagctgc ttgctataac gagagtataa attcaggtat tacgttaatg
     2641 aaaaaaatag acgaaccgca gaaaccagtt attccacaac tcgtggccgg ttggcttgag
     2701 aaatctacgg acccttttac aaaagctgaa aaaatagcgt atttaatcaa atctaaagat
     2761 ggtgattcat attatttctg tgattggttt gtacgagatg gcatagtgac gcaagagcaa
     2821 ggagaagaat tactcgcttg ggcaacaaga caatcatatg aaacactatt gagcctatac
     2881 aacggctacg aggttgagaa agagccgtta tgggcaataa agaatgccga tggaaactat
     2941 cttactaaat gtgctttatg gggaaaagat ggagtaaatt atagttttga atgcaatcca
     3001 tctcatcgat tgcttttcac tgataaagca acagcggatg ctgcagcatt gttggtgaat
     3061 ggaacagtgg aagaggtggt agaaagatga aactaaaaga cggattttac gctagcagtc
     3121 acggtatcgg cggtttaatg ctagatatgc cgacaaagaa ccctaaaaca cgcaagaaat
     3181 caaaattcaa agtcggtgac atggtccgct gtgaagcaga agagttcgtt tatccgttca
     3241 gaggatacgt taaaaagata ctgtcaaact cagcaatcat tcgtattgaa aatacaatga
     3301 agtgtgatcg ttcgactgca aaacacaagc attatttagc tgtagcgaga ttagttgaca
     3361 tggaagtaat caagagcaaa taaaaaaagc cggatcgctc cgactgattc aataaatcca
     3421 acacatttat tatatcacat aaaggagcgg tttgacttga tgcaattgtt acgagaggta
     3481 gatttcaaac agacaagatg taatgcgaga gatgtgctga agaactttcg gcgtttggag
     3541 cggatggcag gtcgctcttt gatagatatt aagtcgccga ttataacgga tatgccgaag
     3601 gcaccgaagc atggcaataa ggcagaagac gcgattattc agatgatgga tatagaaacg
     3661 gagagagatg cgattctagc ggctttgatg gctcttagtc tgattagtcg tcagatactc
     3721 tactacagct tctgtgtgcc agatagcttc tcaaactaca gaattagccg tgaagtgggt
     3781 tattcagaaa gaagtataca acggatgaag tcggaagctc taatagagtt tgcagaagca
     3841 tataaacacg gaaaaataat tgcttataaa taatttggcg gttttttggc ggaatgatgg
     3901 cggtttttag acatttacca gtgttattat ggtagtgtcg aaagattagg aaacaggatc
     3961 gacaaaataa acgaagggag gcaatctccc tcatcgtttt aaacttcttc ttagatagac
     4021 agcaactaat tatgaagaaa aggatgtgaa ttcaactcct cctaaattct tcatcggttg
     4081 ctgtttattt tgattagaca gcagcactca caaaactaaa catgttcttg tcgtctttca
     4141 actgctgctg tctattaatt tatgtattgg agggaagaaa aatgaaatta tcagtagaag
     4201 gcactccaga agaaatacaa gaattgctcc aagctattgg tagtagcaag gagcaaaagg
     4261 taagtgtttc taattcagat attgacggta tttgcaattg tttaaacaga gaaaaaatat
     4321 ctaatcctta ctaagagaaa atatgttcgt taatgaattt ccaattttcc tctgtagctc
     4381 taccttgata atttttttgc agttcacaga tgaataagga atcgttatta tcaagtactg
     4441 tatttaattt ttcacacatt tgatttggat tcaaattagt tttaactaac caaaaagatt
     4501 tttgtagctt tatatatgcc ccaaattctt ttattatttc aaatacttta tcatactttt
     4561 gccctgggtt attaagatca taactaatca tgtagggttt atacatgttg tcttgcacat
     4621 ccttgtttta tatttcagcg gaccactcgc tgataaataa aattatacgc ttagtattta
     4681 ttttcacaat attaatttat cgtaataact tttgagtaaa tagaacaaaa aacctgcact
     4741 agtttccgct agtacaggca gtgacta
//
LOCUS       DAKBVG010000179         4747 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000179 DAKBVG010000000
VERSION     DAKBVG010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.179"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(81..1889)
                     /gene="pepF"
                     /locus_tag="OUD09_002257"
     CDS             complement(81..1889)
                     /gene="pepF"
                     /locus_tag="OUD09_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HCT9693492.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2065..3063)
                     /locus_tag="OUD09_002258"
     CDS             complement(2065..3063)
                     /locus_tag="OUD09_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HCT9693493.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3289..3942)
                     /locus_tag="OUD09_002259"
     CDS             complement(3289..3942)
                     /locus_tag="OUD09_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HCT9693494.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4274..4672)
                     /gene="spxA"
                     /locus_tag="OUD09_002260"
     CDS             complement(4274..4672)
                     /gene="spxA"
                     /locus_tag="OUD09_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HCT9693495.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1391 a         1000 c          743 g         1613 t
ORIGIN      
        1 aaaaaaatac gtcgactccc ttacaagaga gccgacgtat ttttttcgtt tctattcgag
       61 aaaatcaaaa atagaaagcc ttataaacga tcgatcaatt cttctaattc atttagacgt
      121 tgttcaaaca tcgacattgc atcttcaata tatgcagcct gtgtcatatc aactcctgct
      181 tttttcatta cttccacagg ataatcgctg ttacctgctt ttaggtaagc caagtagttc
      241 tctagtgcct ctggttcctg gtttagtatt ttcttcgcca gtgctgaagc agcagaaaag
      301 ccagtcgagt attggaaaac atagtaatta taataaaaat gcggtatccg tgaccattcg
      361 aatttaattt ctgggtcttc ttcgactgct ggaccataat attttgcatt caatttacca
      421 taactatcac ttaggtattc actagtcaat ggcacacctt tttcatcttc cgtatgcatg
      481 aaatgctcga attcagcgaa ttgtgtctgg cggaaaactg ttcctttaaa cccatccaag
      541 taatggttga gtacataagc ccgtacacga gggtcttttt ctgtttccaa taaatactcc
      601 gttaggatat tttcattcgt tgtcgaagca atttctgcta aaaagatgga gtagtcgccg
      661 tacacataag gctggttcga acgagtgaaa taactatgaa cactatgtcc catttcgtgg
      721 acaagcgtaa atagctgatc cagcgtatca tgccaattca ataaaatata tggatttgtg
      781 tcatagcttc ccgaagaata agcaccgctt cgtttccctt tattttcgac aacatcgatc
      841 caacgttcag agaatgcttt ttctacgatg gccatgtatt cttcacccat tggtttcagt
      901 gcttctaaag ctttttcttt tgcttcttcg tacgtaaagg taattggagc ttcccctaag
      961 accggtgtat aaagatcata catgtgcagt ttttccactt ctaataaacg tttccgtaat
     1021 tccatgtatc gatgtaacaa aggcaaatgc ttgtttacca cgtctaccaa agtatcgtat
     1081 acactttcag gaatatgatt attgctcaaa gacgcttctc tggcagagct gtaattacgg
     1141 actttcgctt taaaattatg tccttttata tgtgtgccta aagtagaagc aaatgtattt
     1201 ctaaattgtt cgtaaacact gtacaacccc ttaaatgctg cttcgcgcac ccttcgatcc
     1261 gtgctttcta gcaactgacc atacacgcca tgagataatt ggactatttc accattttct
     1321 ccttcaatcg ttggaaaaac tagatctgca ttattcaaaa cagcaaatgt atcacttgaa
     1381 gcatcaaaga tttcacctgc tccagcaaga agagattcct gctcagcaga taagacatgg
     1441 gctcgattat ctactatttg ctggatataa tggcgataga cttccaattt tggttcttct
     1501 ttgaaatatt gccaaatctg gtcatctgac aattgcaata tttctggttc aaaccaggaa
     1561 acggcttcac taactttcga aaacagactg cttgctcttg cataaagcgc ttggtaatct
     1621 gtatttccag tgtcttgatc gtttttaagg tgcgcatata cataaatgac ttcagtttgg
     1681 cgataaaccc tcaatacgaa ttcaatcgca tttaaaaatt gagaagcgcc ttgatcaagt
     1741 gttcctttgt gtttttcaga ttgttttaac tcttctgata attccaaata tttctcatca
     1801 aactcttggt cgcttgaaaa gatcttggtc aagtcccaag ttaaattttc aggcaattct
     1861 tctcgttttg gcaactgctt tacttccatg aaatccactc cttttcttcc cttagctcta
     1921 tctatcaaaa tagatcatag tctaacatcc acatgtcaca gcatctaact tttctactca
     1981 ttcatgtgtc tgcttccatc aattttagca taactaaatc ttgttttaca tagaaaaccg
     2041 attcctagcc tatttttata cttactattt tttcaaagaa gacaagtgta tgcattcgtt
     2101 aaaaaactgc tgatagacca tctcttcatc aatcatagga aaaagccatt cccttttatg
     2161 ttcttttatt tggcaaagcc atgagttgaa atcaggaact ttctggtttt ccaaggcttc
     2221 ttcgtacaac attcgataaa gaaaaacctg ttcttgtaaa taaaagaaaa accgactttt
     2281 ttgatagatc cacgatgata gatgcaaaag atgtttatga cgcaagtagc attgttcttg
     2341 tatggatact gtattctttt gcttgtgaat aagttggcgt gtcagccatt tcttcccatc
     2401 ttcactcact ttttctattt ttttcatttg gttcatttta atttcgttac tagtgaatat
     2461 ttctttaagg tccctactag aaaaagtcca gcagctactg caaaaggaaa tatgccctga
     2521 tacagtctct gtaatatgat gatacaaata aagcttttgt ttcttgagat cagctttcca
     2581 gcaatgcact cctctttttc tattatagta gcagcaactt ttttctatca atgagaattg
     2641 cgattgtcca agaaaagaat taccaagtat ccaccatact tggtaactgt tattcaaata
     2701 attttgggtt ctttcaataa atctctggtg agacagtcta gaacattgga tttctattgc
     2761 tagattcgcc aacaaaatat ccggcctttg ccgcaacctc ggcaaatagg cttccaaaaa
     2821 tactggttct actgactgtc ccagccaatc catgaagcat tcctttaact gtaagtgctc
     2881 ttttgattct ctttcactaa agaccgaaca gttatctgat ttttgatggg caaaatgagc
     2941 acattttttc tttccctttt tcaaaatcac tctttttcga cagccacgac aataatattt
     3001 ctttcctttt tctgcatgtt tagcaaaaca gcacttatta tattcattca ttgcaagcag
     3061 catttttctc cctctgaact tatttacgtc taagaattta tttacacgaa aaaagagggc
     3121 gtgaaaaagc tattctgcat caagtaataa gaacagctaa acaaaaatag ttcccaatat
     3181 ttttcgtttg gttgggcttg ttaccgcaga tgcaagcttt tgaacacatt tatacgaaaa
     3241 aaagactgcg acacaatttt tgtcacagtc tcttactata atgctgattt atacaaaata
     3301 aaatttcgtt cgctcgatag cattacgcgc catcagacat tgtccatatt cattcaatgt
     3361 ttctggtgta cgatcgctct tttcagcaaa ctccaaaatt cgagcaatct gattctctac
     3421 atccgcttgg ttgagatttt ccatccagaa gtgaacgact aggtaatagc gctcttcgaa
     3481 taaatacaaa tcagtccaag cagactctag ttcgacatct gttgcaagtt ggatcacttg
     3541 ttcaaaatct tcaaaagcaa aagttgcttg tctttcctgt tcttctaggg cgcttccaga
     3601 taattcttcc tccggatcgc tgccaataat ctgctgacga agaaagtcac tcacatcttc
     3661 tgaatcaaca gtagaatctt ctgattgaga aatttcttca tccggaagat ttttactgat
     3721 aaacagttct aatccgtctc cttttggcaa aacttggaaa gtgacagctt cgcttcctcg
     3781 gaactcctct tcgatatcaa cttcttccaa aatactataa aagaaatttt cgatttcttt
     3841 gtgattgcca agtaaatcca aaaatgaaac gccccgttcc tctaaatctt catgaccaat
     3901 tagtacacgg atcgtatttt cattaatatg ttccatttcc attttgctac acctcacttt
     3961 gccacaatgt ttaatataga ttcattgtag cgaaatcagc ttaaatgtaa agaaaaaaaa
     4021 tctcgctgtt aagcgaattt aataaaacca gcggattttt caacctttca agctatctta
     4081 tttaactata acatttttgg cagacgaata ctatatagcc gacttttcag ctaattttcc
     4141 ctctctattt ttcgtgcaat catcatcttc tactaccttt ttatacatct aatcaagaat
     4201 actttttgta aaaacaaaaa aacttggaaa aactatcttt gtaatagtct tttccaagtt
     4261 tcgtattatc cttctataat ccagccatca attgtgcttg acgtaattct aattgtcgta
     4321 catcgcgcgg taggaaccga cgaatttcat cttcattaaa accaacttgc agacgtttat
     4381 catcaatcat gattggacgg cgaagcaatc caggattgtt ttgcacaagt tccaataatt
     4441 cttgtaacgg cagatcatct aaatccatgt tcagtttttg gaatactttg gaacgtgtcg
     4501 agatgatttc ttctgttccg tcttctgtca tttgtaaaat cgcttttaat tcagaactgt
     4561 tcaatggttc tgaaaaaata ttacgttcaa caaatggaat ctgatgttct tgcaaccacg
     4621 cacgagcctt tctgcaggaa gtacaacttg gggaagtata taatgttaac aacgtgcacc
     4681 actccttttc gtgataagaa tcactttttt gtaacagaag atcataataa tctcccatct
     4741 gtttcta
//
LOCUS       DAKBVG010000180         4733 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000180 DAKBVG010000000
VERSION     DAKBVG010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.180"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            14..685
                     /locus_tag="OUD09_002261"
     CDS             14..685
                     /locus_tag="OUD09_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCT9693496.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            744..1184
                     /locus_tag="OUD09_002262"
     CDS             744..1184
                     /locus_tag="OUD09_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693497.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1196..1864
                     /locus_tag="OUD09_002263"
     CDS             1196..1864
                     /locus_tag="OUD09_002263"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCT9693498.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1928..2551
                     /locus_tag="OUD09_002264"
     CDS             1928..2551
                     /locus_tag="OUD09_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HCT9693499.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2607..2993
                     /locus_tag="OUD09_002265"
     CDS             2607..2993
                     /locus_tag="OUD09_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HCT9693500.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3206..4597
                     /locus_tag="OUD09_002266"
     CDS             3206..4597
                     /locus_tag="OUD09_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HCT9693501.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         1573 a          782 c         1009 g         1369 t
ORIGIN      
        1 aagggggaga aaaatgaatt ttttagttgt tttaaaagat actctgattg agcgatctac
       61 atttctatat gttcaattgc aggaaaataa aacattgctg catttttcac cagagttcca
      121 tctagagggg ctaacattag gagaaatcta tcaagcagaa gggctgtcgg ctgttttgca
      181 cttttttaag gtagaattgg aattaccagt aaaagattat atcgagcttt ctcttgaatc
      241 ttgggatcga gcagtggttg aactgtttcc agaaggttta atgatcgata caaacgatgg
      301 gaagatccat ctgactgcag cagaactgca aaaacaaatg cgttatcaaa tagatgacga
      361 aaattctttt tctattttta gacgtcagca aaagattcta aaaagctttt taaaagtgat
      421 cagtaaaaaa cggaatctga tcaaaacgac acagctgttg aaaaaatatc caggtctgct
      481 tcacacgtct attcaatttc ctcaattgat tacgatgatc catcgtttta tacgtatgca
      541 acagctacca tctaagaagc tgtttcttcc attgacggac acatttcgag tagtcaacag
      601 agaagataaa aagaaagtga ttgtgattga ctttaccaga aatcggaatg tccttcacca
      661 agttgcaatg gaaaaggcca attaaggctt tttctttttg gatttttttg tcataataaa
      721 tattagatga tggaggagat aatatgatgc aaacatggtt agaaaggcta atacgatttc
      781 agcagaacta tcacaaactt atgcagggaa gatatgcgaa atttgatcag ttgaatcgcg
      841 gtctattgat cgctgcgctt gtctttagtt tgctaagtcg ttggttgccc tatagaatcg
      901 gacaattgtt gttcatcgtc tttttcggat ggatgatatt tcgtttccta tcaaaaaaaa
      961 tctaccccag actaaatgag aatcaacgtt atttgaaata tcttgataga gtcaaagaaa
     1021 aatatcatca gataaaaaac aaaaagcaag tcaaaatgga tcgaaaaagt tatacttttt
     1081 ttgagtgccc gaattgtcat caaaagcagc gtgcacctaa aggaaaagga cgaatacgtg
     1141 tgacatgcaa aacatgtggt ataaaattcg aaacgaatgt ttaagggaga aaagaatgaa
     1201 attagtatta ttacgtcatg gcgaaagcga agcaaatttt gaaaattact ggacaggatg
     1261 gctagatgtc gccttaacag aaaaaggaaa agaacaagca gcagttgccg gtcaaaaaat
     1321 gcttgaagcc ggacttcaat tcgatgccgt ttatacctct gttttgaaac gtgcgattct
     1381 taccggacaa gtcgcactag aagaaatggg tcagttatgg cttcctataa taaaaacatg
     1441 gcgtctaaat gaacggcatt atggggatct tgtgggtaaa aacaaagata agatgaaaga
     1501 aatatacgga aaagatcaag taaaaacatg gcgcagagga tatacggaag ttccgccgtt
     1561 aaccgcagaa aatagatttg accgcagata tgatcacttg gatccgcatt tgatcccata
     1621 cggagaaagt ctggaaatga cagtgaatcg gatcattccc ttatggcaag atcatctagc
     1681 accaaaacta aaagatcggc aagatttact agttgtgggt catggtaata gcatccgagc
     1741 actgacaaaa tatttagaag atgttcctga agatcaaatg gatacgatcg atatcccaaa
     1801 tgcacaacca atccagtata cattagcaga tgatttgaca attctggaca aaaaaatact
     1861 ttaaagaaca cctttatcta aaagtgagag atagaaaaaa gaagcaaagg aatggaacaa
     1921 aaggaacatg aaaaattata tatgggactt tgatggtaca ttgtttgata cgtatcccgc
     1981 aatggtagat ggtgcatggc aagcactaaa agattttggt atcagtatgg acaaaaaaga
     2041 aatctacttc aaaatgaaaa agtattctac tagttatttg atcaatgaat caaacttgaa
     2101 tgccagagag tttaatgaat tatttcaccg atatgaaaaa gaaagtacgg aagtttcccg
     2161 tccatttcca gaaacgaaac aagtactgga aatgctaaaa gacaacggcg gtcgtcattt
     2221 catcctgacc caccgtttga cagaatcaac atggggcctg ttaaaagaac atcgattggc
     2281 acatctgatt gaggaagtag tagggatcga tcaggacttt cctcgtaaac ctgatccagc
     2341 atctttaaat tatttgatcg atacttttca tttagaaaga acagacacga tgatgatcgg
     2401 cgatcgtcgt ttagatatcg aagcagggaa aaatgcagga gtcgctactt gtctttacga
     2461 catcgatcat tttctgggtg agatcccggc agattatgtg gtaggaaatc tgaatgaaat
     2521 ccttaccttg cagagcatca ttaaaaagta gtatcccctt tcaaaggaga tatgttataa
     2581 ttttcataaa ggagtgataa aattatatgg aaatcagaag acaaaaagaa gttgtagaag
     2641 catttcatta tgatatgaga ttgccgaacc aagaagtaga aacagatttg aaagtaggct
     2701 tctcgccttt aaaagaaacg atcgaaaact atccgaaaga acatagcatc gtaggagcac
     2761 gcttagagtt ccgtttagtg tttgatacat atgttttatc tggaagtgtg agtcagatca
     2821 atcatatcct caatcggaaa attgaaggtc aaaatgatgt cacacaagaa gaagtggacg
     2881 agctagtagc accgttattc gatatcgtac gccgaatgtc ttatgaggtg acagagatcg
     2941 ccttagatca gcctggggta aaattgaatt ttcaatcaga aaaacaagaa tagacagtaa
     3001 aaacatacct gcgatagatt aaagctatca caggtatgtt tttttattac taattgtaag
     3061 caaaaagaat gtcatttcga taaaaatcgt ctaaaatata aaagtcactg aaaaaaattt
     3121 tttcaggagg atggttgaag aatcattctc gggaaccttt acttccttcg atggacttgt
     3181 cattaataat atgaggagga gaaaaatgac aacactcatc acagttttag tggttctagt
     3241 ttttgcggca ttattatatg tgttctatcg tatgcaaaag cgccatacaa aattttcaac
     3301 acgcgtcttt acagcattag gagttggtat cgtctttggc ggtctgcttc aattgatctt
     3361 tggtgtagac gacaaagtcg ttactcaatc aatggaatgg atcgggattg taggtcaagg
     3421 atatgtatca ttcttgcaaa tgctagttat gcctttagtg tttatttcga tcattggtgc
     3481 attcacaaaa atgaaagttt ctgaaaagct agggaaaatt agtgcgactg tattgacttc
     3541 tttacttgga acaacagcta ttgcagcatt gatcggaatt ggttcaacaa tgctgttcgg
     3601 actccaagga gctaaattca cacaaggagc tgcagaaaca gcacgaatct ctgaactggc
     3661 aacacgtcag gaaacagttg aaaacttatc gattccacag cagattttat cctttatccc
     3721 taaaaacgtt tttgctgatt tagcaggtag tcgtgcaaca agtacgattg gagtcgttat
     3781 tttcgcggca tttgtcggaa tcgcttatct aggcgtgaaa agaaaagcgc caaaagaagc
     3841 agcattcttt tcagatttag tcgacagtct gtataaaatc attatgcgga tcgtgacgct
     3901 tgttttgcgt ttgacacctt atggagtatt tgctttgatg acaaacgttt tggcaacaag
     3961 tgattttgca gctatcgtca atttaggtaa atttgtcgtt gcttcttacg tagcattgct
     4021 tgctgttttg attgtccact cactgatttt gattggtgca aaagtcaatc caatcaacta
     4081 tttcaaaaaa gcctttccgg tattgagctt tgcgtttact tcacgttcaa gtgctggagc
     4141 tttgccattg aatatcgaga ctcagacaaa agctttagga gtagatgatg caacagctaa
     4201 tttctcagca agttttggtt tgtctattgg acaaaacggc tgtgctggcg tttatccagc
     4261 tatgttggca acgatcgtcg caccagcagc tggagtgaat gtctttagtt tggaatttat
     4321 tttgatgctt gtggcaattg taacaatcag ttcttttggt gtagctggag tcggtggggg
     4381 cgcaacattt gcttcactga tcgttttagg ggcaatgaac ttgccagtag caatcgttgg
     4441 gttagttatc tctgttgaac cattaatcga tatggcacgt acagcagtaa acgtcagcgg
     4501 tagcatggta gctggtatcg tgacaagtgc acgcatcaaa gatttagatc gcgatgtcat
     4561 caatgatgat tctaaagtga tcgatgtgaa tgcatagtca aaaagaggtt gtgatagaag
     4621 tggtcagctt taagaaataa gaagaagttt cctaaaaata gtttctcaaa tttttgtgaa
     4681 atttcggctt atttccgaga agctacttct gaaacaccgt gaataagagg ttg
//
LOCUS       DAKBVG010000181         4612 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000181 DAKBVG010000000
VERSION     DAKBVG010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.181"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            10..1200
                     /locus_tag="OUD09_002267"
     CDS             10..1200
                     /locus_tag="OUD09_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HCT9693502.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
     gene            1334..3523
                     /locus_tag="OUD09_002268"
     CDS             1334..3523
                     /locus_tag="OUD09_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCT9693503.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            3520..>4612
                     /locus_tag="OUD09_002269"
     CDS             3520..>4612
                     /locus_tag="OUD09_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCT9693504.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPY"
BASE COUNT         1652 a          713 c          836 g         1411 t
ORIGIN      
        1 gacttcccta tgaacgattt tactacagaa attctaaaga ctctagcgaa caaaggcgat
       61 ttgaatgaat tattccgtgt ccatttggag aaagcagtca atacgcttct caaaacggag
      121 ttaacggctt tcctagatta cgaaaagtat gatcgcattg gttttaacac gggtaattct
      181 cgtaacggct cctatgaccg tacggtcaag accgagtatg gggaacttca tctccagatt
      241 ccgcgcgacc gcaatggtga gttcaagcaa cagactgttc ctgcttatag acggacgaat
      301 gacacgttag aggagaccgt cattcacctc ttccgaaaag gtattaccat gtcggaaatc
      361 gcagacttga ttgagaaaat gtatgggcat tactacacgc cccaaaccat gtccaatata
      421 acaaaatcat ttacagaaga ggtaacggcg tttaatgggc gggagcttca tgaccgttat
      481 gctgctattt atatggacgc aacgtatatt ccgttaaagc ggaaaaccgt cgccaaggaa
      541 gctattcata tcgcagttgg cattcgcccg gacggatcaa aggaagtatt gagctatgcg
      601 attgcaccga ctgaatccat cacgatttgg gaggaaattt tattggacct tcaagagcgc
      661 ggtttgaaaa atgtcctcct gttcatcacg gatggcttaa aggggatggt aggagcgatt
      721 agtcggttct atcccaaagc tcgttttcaa cattgttgtg tacacgtttc ccgtaatatc
      781 agtcataaag tgcgtgtcga tgatcgtaag gaagtctgtg atgattttaa aatggtgtat
      841 caagcctcat ctaaagaggt ggcgttggaa gcacgtggta cttttgcgga aaaatggaaa
      901 accagctatc caaaagtggt tgaatcgatt ctttcgaacg atcacttgct cactttctat
      961 gatttcccct tggccatacg caagagtatt tattctacga acttgattga atcctttaat
     1021 aaacaaatca agaaatacag ccaccgcaag gaacagttcc aaaatgaaga gtcgatggaa
     1081 cgtttcttag tctcgtcttt tgatacttac aaccaaaaat tcctaggtcg cagtcataaa
     1141 ggtttccaac aggccgaagg cgagctcgaa caaatgctaa gccaactgat tgagaattag
     1201 aaacaatctg cagtagggaa gaatcattta cacaaaatta ttgacgctcc cttataatga
     1261 gaacttttac taacataatt tatgataaac ttactatata atcatattaa ttaaatagtt
     1321 aggagatctg cttatgttta tatctactct tcaaattcga aattttagaa attatgaatc
     1381 cgaaatcttt cgattcgata atgagacaaa tactattata ggtgagaatg attcagggaa
     1441 aaccaatgct cttactgcat tgagaatctt attagatgat agttattact atagtagtaa
     1501 aacactaaaa gaatcagatt tttttcatgg aattcaaaat gggtggcaag ggcattggat
     1561 tattatctct gcgacatttg aaggaatcag cgaagaagaa tttgacaatg agatttgtgc
     1621 ttccatttct ttaaattctg aaagtcaaac aatcttagaa gaattgattt caaatgcaga
     1681 taaaggtgta ggtagtattt ctctatttat tagacctaac aaagctatta gaaagcaatt
     1741 atttgatatt tccactgaat tagactctca tcaatttaac gaatttagaa attctattcg
     1801 tctatccgac tatgaatttt actacacatc taaatcaaat ctaaatttct gtatcaatga
     1861 aaattatgat aaattggtag gaagactcaa tgacaatata gctagtaatc ctgaagaaga
     1921 cgatgaagct ctgttgggct caagaattga catgtcagat attttcaagc atatttcggt
     1981 tgtttatgtt gacgctcttc gagatgttct tagagaaatg aaaaacaatc gaaacccagt
     2041 caaaagaatc atggaaacaa ttgaatcaaa aatatcatca gataatgttg agtcattaaa
     2101 aacaattata cagcaattaa atgagactat taccagtgtt cctgaaatta ggaaaattgg
     2161 cgaaaatatt aatcggcaac taaatagcat aattggttca gtgtattctc ctaatcttct
     2221 tctttcctca actatgtcag atgatatggg ttctttagca aaatttcttt caatgaagcc
     2281 agaacaaaat atcgacttag acttactagg gttaggacat ctaaatatga tttatttagc
     2341 tttgaaaata gtcgaatttg aagcgtgtcg ttcaagagaa ctacttaata ttatgttgat
     2401 tgaagaacca gaagctcata ttcatcatca tattcaaaaa actctgttcg aaggattaaa
     2461 tcttcaaaaa aactatactc agattctcat gaccacacat tctgtacatt tagctgaagt
     2521 gtctgaaata tctcggatga atgtattaaa atcgtttaat ggcaaatcta tcgttatgaa
     2581 tccagcaaat aatctgaact catttgctaa agaaaagtta aaaaagaaca atctaaatct
     2641 aataaacgca gtagagagat atttagatgt taaaagaaat ggattgcttt tttctaaagg
     2701 tgttatctta gttgaaggag atgctgaaga aatacttatt cctcaaatag taaaaaaatt
     2761 atttggtata aacttagacg aacttggaat aggtttgatt aatgttggta gtacttcgtt
     2821 tgaatatatc gctagcttgt ttgacgatga tagaatacag agaaagtgtg ctattgtaac
     2881 tgatttagat gaacaggcaa tcccatcaga acatcgattg tataaaagca ctgcacaaga
     2941 gaaaggtcaa gcaagaaaag aaaaattatc ctcccttttt aatgaaaaca attgggttaa
     3001 ttgtttttat gctaatacaa catttgaaat tgagttttta gatgtgaata aagaaaatat
     3061 tctaacttat gtcagtccaa ttatagatga cgcattcaaa gataaaaaaa caataactgc
     3121 ttataaagaa gaaatacaaa gtgaacaata tttagaaaga aatttagcga tgttaaaact
     3181 agccaatcat gttggtaagg ggtggtttgc aattgagtta ggtgaaaaaa ttgacttttt
     3241 aatcaaaatt cctaagtata ttcttaatgc tattgctttt gcatgtcagg aaatagtaga
     3301 tctctcaatt tataaaaaaa ttctactcca tacaatcgac ttgtacgagg aaactacaga
     3361 attgtcagct ttatcagcta gtttaaaaac aagtgataca gctgatttga atatagcaat
     3421 aatttcttta ttagagtact ttggtaaaga tgaggatgtt gtgtatctaa tcaatcagat
     3481 agaaaattac acaaaaatac tttatgctga ggtttcaaaa tgacaaatat cttcgaaaaa
     3541 atcgtaaatg atttaaattt agaacaaaaa gatgttgtgg aaagtgaaga taacctttat
     3601 gtaaatgcat gtcctgggag tgggaaaaca agggtactta caaggaaaat tgcttaccaa
     3661 gcaattaaac acgatggatc actaaaaaga ataattgcta tcacatacac taatagagct
     3721 gctgaagaaa ttaaggaacg cctaagttta cttagtatag atgatgatat taatatttgg
     3781 gtggggacaa tacatcagtt ttgtcttgaa tttattattt atccttttaa aatgaatctc
     3841 tcaagagtat caaaaggatt tacaattatt gatgattata ctcaaagagc ttacgtcagc
     3901 caaattaata atatgctgaa tttaaatctc aaaacgtatg aactgaacaa aattgatttg
     3961 tcactttctc atgattttaa aattaatgaa gttcagtata ctcaagtttc tgaaatgtat
     4021 cacactatgt tggaaaaaaa taaagaaatt gattttgata tgattttact agtagcgtat
     4081 aaaattttaa ctgtaaacag taacgttgca cgtaatattg ctagtaatat aagggcgatt
     4141 tatgtggatg aatttcaaga tactagagaa ttacaataca atgtaatagc taaattactt
     4201 caaaacaacc ctcaaattca gtcaatgttt gttggagata ttgaccaagc aatttatgga
     4261 tcattagatg gtatagctaa atcagttgaa gaactagaaa tgctaactag tcacactttt
     4321 caatcaaaga cgctacatgg ctgttacagg tctaatcaga gattagtaga cttctactct
     4381 aattttcaga gttgtcctta cgagattgaa agcagaggga ataataaaaa cgaccgtggt
     4441 tttataagtt atgattgcaa agttactaag gaggatttac ccaacataat aaaaaatata
     4501 ataaaggaaa agattgaaag cggcattcat gaaaaagata tttgtgtact tgctcctcaa
     4561 cactatcctc tgttttcact gggtgagtat ttaaagaaag aattaccgta tt
//
LOCUS       DAKBVG010000182         4529 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000182 DAKBVG010000000
VERSION     DAKBVG010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4529
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.182"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            14..496
                     /locus_tag="OUD09_002270"
     CDS             14..496
                     /locus_tag="OUD09_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HCT9693505.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSEHMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLLNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            533..1543
                     /locus_tag="OUD09_002271"
     CDS             533..1543
                     /locus_tag="OUD09_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HCT9693506.1"
                     /translation="MISYPLLERNTTIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGINLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(1658..2371)
                     /locus_tag="OUD09_002272"
     CDS             complement(1658..2371)
                     /locus_tag="OUD09_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693507.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSSDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPTHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
     gene            complement(2561..3151)
                     /locus_tag="OUD09_002273"
     CDS             complement(2561..3151)
                     /locus_tag="OUD09_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HCT9693508.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQKEGWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            3674..4345
                     /locus_tag="OUD09_002274"
     CDS             3674..4345
                     /locus_tag="OUD09_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HCT9693509.1"
                     /translation="MEPKDKYLDYARVNKDKFIETIIKGKIPDDEKDAVFMAGSPGAG
                     KTEVALGLAENYDNHVVIDADYFRAQFPEYNGKNSSVFQKASSWLVEQSLKYVLEHGY
                     SFILEDTFAILSAEKNIIRALKNNFRVTIFYVYQDPKVAWDFTRKRELAEGRHVPKEM
                     FINAFFKSRENIEKVKNRHPEVVLHIMIKDYQNDISEVHYAADNIQLVLPNTIYFKRV
                     GGGIT"
     gene            4342..4503
                     /locus_tag="OUD09_002275"
     CDS             4342..4503
                     /locus_tag="OUD09_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693510.1"
                     /translation="MTEKEQVQQIVKKYNKSIADLSENASAKEFKTVMKYVADEANRK
                     QRKLVGLDK"
BASE COUNT         1576 a          734 c          860 g         1359 t
ORIGIN      
        1 aagagacata aaaatgattg acttggcaaa gaaattctta cacgaaaata tatattttga
       61 gttgtatcat catcgagcca tttatacaaa tgaagatgct ttggtaataa agaaagaaca
      121 aggatttaca ggtacagaga ccaaaagctt attcttaaaa gataaaaaag ataataacta
      181 cgtatatctg acttttacta cgaaacaaac ggatttcaaa gcattgggga agatcgtcgg
      241 taaaaggctt tctattgttt catcagaaca catggaagaa caaacgggac aaaagcaggg
      301 cgctgtttct ccgtttggtt atgaaaaaca gattccgatt attattgatg aagagctctt
      361 attaaatgaa aaacttgttt ttgcaccagg tagaccagat caaacgatgg tcatccaaac
      421 ggttgactta aacaaaatca tccagctact tgagatgccg atatttttga tgccggcaaa
      481 cgaaaaaaac aattaaccaa gtaaaatagt gcatacttgg aggtgaagca acatgataag
      541 ttatccatta ttagaaagaa atacaacgat tggggttact gcaccatcct caggcgtatc
      601 agaggaacac cattcgttac tcgaatcagc tttaaagaga atggaagaaa aatctttcca
      661 agtagttact ggaaagacgc tttggacgca agataaggaa aaatcaggtc cagcaagaaa
      721 acgagcagat gaattcaatc aaatgatgca agatgaaaat atccaaatga tctttccgcc
      781 ttggggagga gaattattga ttgagattct cgattttgtc gattttgatg ctctacaacc
      841 aaaatggata gtaggttatt cagatattag tacgttgcta cttgctgtca cattaaaaac
      901 agggatagcc actgcacatg gaatcaacct tattgattta agaggagagt acttggatga
      961 aacgacagca atgtgggaaa gtgtactgaa aacaagagaa ggagaatcga ttctacagca
     1021 accttctgaa aagtatcaaa aagagtggcc ccatgagcat tcatcatctt ctgttttcca
     1081 tctcactgaa ccaacaaatt ggcaatcagt cagcggagat gcagtaagga tgaagggacg
     1141 attattaggt ggctgtattg atacgatcag tcatttaatc ggtacccctt atggagatgt
     1201 ccaatctttt aaaagaaagt ataatgatgg aaagccgatc ttgtggtttc tagaaaattg
     1261 tgagctatcg tcagttgatt taagaagatc acttgttcag atgaaactgg caggatggtt
     1321 tgatggcagc tcggggatca tgttcggtag aagtgcagca gaggatttgt tagattataa
     1381 aatgaaggat gtatacgaag atctgtcaaa ggaattaaaa ctaccgatct tgtatgatat
     1441 tgattgtggt catgtaccgc cacaactgac attgatcaat ggtgcctatg cagaagtagc
     1501 ggtagaagat agaaaagcga ctgttttgca aacgtttatt taaaaaattt tttcctgcct
     1561 tggagtttat caaagaccaa caaatctaaa tagaaaagaa aatgaaagaa gctgacagta
     1621 ttttgccagc ttctttttgt atgatctatt agaaatttta attatccata actatcacat
     1681 tgatcagctg tttcacatca tttggattac ctgtaggaat agctactaca gatgcattat
     1741 caataatttc ttgagttgtt tctctaatga acgctggata agatgtttga tctgcaggca
     1801 tttgccaatg tgtaggaact ggataactag tgattgtccc gtcatggtta cttgaataca
     1861 ctaaaactcc ctggtaactg tatttcccag tgagcataac cacatctttc ggatagactg
     1921 cactccctgc atcataaggg gcgataggtg ttcctgcagg ataatgaata acatgtagtt
     1981 cgaaatcact cgatgtatct ctaaaagtat tacctctaat agctagccaa acacgtgcgt
     2041 actcaatttg tagatcagaa tatcctgcta acggatctat tgactgagaa gactgtgagg
     2101 aagttgtgtc tattgttgaa gaagaaatat ttgtctcaac tgtactacta gatgtagaag
     2161 aagtattggg ctctgagact ttactgcttg ttgaagatgt attcgtttca gtagaagctg
     2221 atttctgagt acttgatttt tcagctgaac tggtttgtct gattgaactt ttgtctgtgg
     2281 tcgcagatgg atcactattt tctttactag gagacgtaca tcctacaagg aataaagaga
     2341 ccaacaataa aataaaacct aactttttca tataaaatac ctcattattt ttttaatacc
     2401 agtatagcgt aatcctgaat ttttgaatcc gtaaaacctt gtctatgaat ataaatagag
     2461 tatatcttct ttgataaatg tcagaaaaag tgtgacaaat aaccatcagt aaaattactt
     2521 gtcatactat tgagaaggag tatttaattg aagagaatga ttaggagaaa ggatctccgt
     2581 actttttata gtaatcatag aatccaagtt tgtatagacg aatcgttttt tctcttattt
     2641 cttctgtttc aaaatggact gcttgaagaa tcaagtctgt gaattctata ggagctgttc
     2701 cattcgcagt aatcaaatta cgatctttca cagcttgttg ttctataaaa tcttctttat
     2761 tctgataatt ttccgattct aaccaaggaa attgcgcatt tccggtgtgt ttgtaacctt
     2821 gcaataatcc gctctgagct aaataatcaa ctgccccaca aatagcagct actggtacgt
     2881 ctgtttttaa caatttatcg attatttgat ataaatgatg attttttatg tcccatgaat
     2941 tgccaccgat cataataaaa aggtcaactt tttcaggtat ttgttcgatt aagtaatcga
     3001 tttttgtttt taatcctcca atggaagtga cctctgattg gatagaagca gttttgtttt
     3061 gccatccctc tttttggttt agtgtgctag taatataagc accttcccaa tcggcatatt
     3121 cctctaatag aaacgtaatc gcagttttca ttttcaagct ctcctttact atctatgaca
     3181 aggacaacag ttttaacata cagacattca ttaaatttgg atagcgacag cgaaaacaac
     3241 aattcttaag ttttagacag agtatacaga cagtcaactt aattaaatac gaactaaata
     3301 aaaatgacag gtaatcattt ttgtttacca gcattataac attttgcata catggatgac
     3361 aaaagaacca attcccactt gcttgagtac aatcttaaaa gaaactggaa ggcaaaaagt
     3421 aattatagaa acgaaaaata aatcatatcg ggcactcaga catgaaaaca accctagaaa
     3481 tatataacta gtccaatgaa aaaacagcga atgccgtaga aaacaaggat tctgcgtaca
     3541 ttcatatctc attttgcgag catatctgct attactgtga ctaaatgtta atatggttaa
     3601 aatgaccgtt atatttttta tataaatatc gtataattag tatgaatata gaagaaggaa
     3661 agaaggtgca atcttggaac ctaaagacaa gtatttagat tacgctaggg tcaataaaga
     3721 taaatttatt gaaacaatca tcaagggaaa aatccctgat gatgaaaaag atgcggtatt
     3781 catggctgga agcccaggag caggtaaaac agaggttgca ctaggtttag ctgaaaatta
     3841 tgacaaccat gttgtgattg atgcggacta ttttagagca caatttccag aatacaatgg
     3901 aaagaactca agtgtttttc agaaagcttc ttcttggtta gttgaacaat cattaaaata
     3961 tgtactggaa catggctatt ctttcatact agaggatact tttgctatcc ttagtgcaga
     4021 gaaaaatatt attcgagccc ttaaaaataa ctttcgcgtt acgattttct atgtttacca
     4081 agatcccaaa gttgcctggg atttcactcg aaaacgtgaa ctagctgaag gacgtcatgt
     4141 gccaaaagaa atgtttatca atgcgttttt taagtccaga gagaatattg aaaaagtcaa
     4201 aaatagacat cctgaagtag tccttcacat aatgattaag gattatcaaa acgacatttc
     4261 tgaagtgcat tatgcagcag ataatattca attagtacta cccaatacaa tatacttcaa
     4321 aagagttgga ggaggaatta catgactgag aaagaacaag tacaacaaat cgtgaagaag
     4381 tataataaaa gtatcgctga tttatcagaa aacgcctcag cgaaagaatt taaaacagta
     4441 atgaagtacg tagcagatga agcaaatcgt aagcagcgaa aactagttgg gttggataaa
     4501 taaatttttt aaaccatatc cttgtaaag
//
LOCUS       DAKBVG010000183         4518 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000183 DAKBVG010000000
VERSION     DAKBVG010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4518
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.183"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(<1..612)
                     /locus_tag="OUD09_002276"
     CDS             complement(<1..612)
                     /locus_tag="OUD09_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="HCT9693511.1"
                     /translation="MLNERFLKYVKGWYFIYFGFILMLRAIGPYLLLPTRYDTLLFWP
                     AGIAGVVLIIIDAIIAFREKNLKKYDWLLIAFIGAMLVSSLMNLKYGYSENIKLIMWQ
                     LIFFFMIYQFGKDYSSNFFFKVFTWILSLVWFLANLVSLYWFVIQYGIKIPIKYKYYP
                     VRLGWLGNRLFGVYTDPNFGAVISLIVIIIAIYYLVTYKHLNLL"
     gene            738..1148
                     /gene="tagD"
                     /locus_tag="OUD09_002277"
     CDS             738..1148
                     /gene="tagD"
                     /locus_tag="OUD09_002277"
                     /EC_number="2.7.7.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HCT9693512.1"
                     /translation="MKRVITYGTFDLLHYGHINLLKRAKQYGDYLIVALSTDEFNWNA
                     KQKKCYFSYEKRKQLLEAIRYVDLVIPEENWEQKVKDIKEYHADYFVMGDDWAGHFDE
                     LEQLTDAQVIYLPRTPEISTTQIKNELNSLNSDK"
     gene            1324..1737
                     /locus_tag="OUD09_002278"
     CDS             1324..1737
                     /locus_tag="OUD09_002278"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCT9693513.1"
                     /translation="MISISHSIKELFTIKDKNIEINSFQETFYKDKKAIVIEGPLVRT
                     FRHCLFCKESAKQIEKNGKKISMIILNRSGNKQTYLRLRKQHYHCQSCGKYFTAHTYL
                     VAPSCFISKQVHYTILEELTERQALKTIAKRCDFQ"
     gene            1894..2151
                     /locus_tag="OUD09_002279"
     CDS             1894..2151
                     /locus_tag="OUD09_002279"
                     /inference="COORDINATES: protein motif:HMM:NF013753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCT9693514.1"
                     /translation="MTFCPAGEKKDLIAYFMRFTRKAHMGVRYIVTDMNAPYFSLVKE
                     PFPNAQIIIDCFHIGQHLNKFFDRVRKRVMKQLNQKCQRHL"
     gene            2238..3476
                     /gene="istA"
                     /locus_tag="OUD09_002280"
     CDS             2238..3476
                     /gene="istA"
                     /locus_tag="OUD09_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HCT9693515.1"
                     /translation="MRKDIREGVMIYVINEIKPNYAALAKQYDCDYRTVKHAYEEAQV
                     KESKPPERKKRPSKLDPYREIIQDKIKDQCSAYSIFKFIEHKGFGGSYSLVKTYCRQI
                     KQAKVHKATVRVEHSPGLSAQVDWKEDMRLISNQGEIFEFNIFLYVLPYSKKKYVTLT
                     FDRKQDTLFECLDDAFYQTGGVPKEIWFDNMKTVVDHSRSQFSKPHFNKRFYAFSKDA
                     GFTPIACRPFRPQTKGCVEALARTVDRLRVYNHEFYDAVELCHLVNNLCVELNNEVSQ
                     STDQTPDERWQTNEKEHLHPYQKNLLNPYFEDFIRRKVTKEEMVIFRKCRYSVAPRYI
                     GKEVEIELSDKEDQVQIYYNGELIRSHPLTTKQLNYHEEDYFQILKSDVMSHKEDDGI
                     RAYTRESLKQYDLVEVADEE"
     gene            3466..4206
                     /gene="istB"
                     /locus_tag="OUD09_002281"
     CDS             3466..4206
                     /gene="istB"
                     /locus_tag="OUD09_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HCT9693516.1"
                     /translation="MKNSHYQELMTNLETLGLNHMREYVPNYIEVANREELSLTEALL
                     ELTRREVDHQNKQKMNRVVARARFSKRTSLEEFDFAFQPSINKREVLDLRHMGFVEKH
                     ENLVFIGSPGVGKTHLTIALGIEACRQGYRTLFITCHELLLRLRTAYEKGTLERVLKR
                     YARYDVLIIDEIGYLPIQKQEADLFFQLLAMRYEHRSTIITTNIVLSRWGELFQSSEI
                     AAAILDRLVHHVKVFKITGKSYRMRDKI"
BASE COUNT         1545 a          860 c          814 g         1299 t
ORIGIN      
        1 tagaagattt aaatgcttgt aagtgaccaa ataatatata gcaataatga tgacgattaa
       61 tgatataacc gcaccaaaat ttggatctgt atatacacca aataaacggt tgcctaacca
      121 ccctaaacga actgggtaat acttatattt tattggaatt tttatgccat attgaatgac
      181 aaaccagtat aatgatacta aattagctaa aaaccatacc aatgaaagta tccaggtaaa
      241 tactttgaaa aaaaagttac tactatagtc tttgccaaac tgataaatca taaagaaaaa
      301 gattaattgc cacatgatta acttaatatt ttcgctatat ccatatttta aattcattaa
      361 actgcttact agcatcgcgc caataaaagc aattaatagc caatcatatt tttttagatt
      421 tttttctcta aatgcaataa ttgcatctat aatgatcaag acaactcccg ctattccagc
      481 aggccaaaaa agtaaagtat catatctagt aggtaatagt aaatacgggc ctattgctcg
      541 taacattaaa ataaaaccaa aataaataaa ataccaacct ttcacatatt ttaaaaatct
      601 ttcattcaac atttttcctc catccaccta tttctcataa atatttctta tcctttttag
      661 gataaactgg tcctttcaat tctaacaaaa aaatgctaaa ctaacaatgt atattaatga
      721 atttcgagag gtgaaagatg aaaagagtaa ttacttacgg tacctttgat ttgcttcatt
      781 acgggcatat caatttactg aaacgagcaa aacaatacgg agactatctt attgttgccc
      841 tttctacaga tgaattcaat tggaatgcga aacaaaaaaa atgttatttt agttatgaaa
      901 aaagaaaaca gttacttgaa gctattcgct acgttgattt agttattcct gaagaaaatt
      961 gggaacaaaa agtaaaagat ataaaagaat accatgcaga ttatttcgtg atgggcgatg
     1021 attgggctgg acattttgat gaacttgaac aactaacaga tgcacaagtg atttatttac
     1081 caagaacacc tgagatctct acaactcaaa taaagaacga acttaattct ttaaatagtg
     1141 ataaataata ttttttaaac aataaaagaa caatatatct ggataagacg taaaaaaatg
     1201 gctctataat ttctaggact gatagaattt tctgtcggtc ctagattttt tgtaaaaaaa
     1261 aacagacata aaaaaattcc gatagaatag agtcgtctaa aacatcaatc tgaaaggaag
     1321 tttttgatat ccatttctca ttctatcaaa gagctcttca ctattaaaga taaaaatatt
     1381 gaaattaatt cttttcaaga aactttttat aaggacaaga aagccatcgt aatcgaaggt
     1441 ccattagttc gcacttttag acattgtcta ttttgtaaag aatctgcaaa gcagatcgag
     1501 aaaaacggca agaagatttc aatgattatc ttaaatcgca gtgggaataa acaaacctat
     1561 ttgcgtctta gaaaacaaca ttaccactgc caatcatgcg ggaaatattt tactgctcat
     1621 acgtatcttg tcgctccttc atgttttatt tcgaaacaag ttcattacac gatattagaa
     1681 gagttgaccg agcgtcaagc attaaagaca attgcgaaac gttgtgattt tcagtaacga
     1741 cagttcaacg gacattatct gctctatcgg caacgctgcg acagaagcta gactatttac
     1801 ctcattgttt actattcgac gagtttcgct ctctttcaat tttccatggg aagttctcct
     1861 tttcttgtat gaattgagag acagggaagc tctttgacat tttgtccagc cggagaaaaa
     1921 aaagatttga ttgcttattt catgcgtttt acaagaaaag ctcatatggg ggttcgatac
     1981 attgtcacag atatgaatgc cccttatttt tcattagtca aagaaccctt cccaaatgca
     2041 caaatcatca ttgattgctt tcacattggg caacacttga acaagttttt cgatcgtgtt
     2101 cgaaaacgtg tgatgaagca gttgaatcaa aaatgtcaac ggcaccttta aaatgtagga
     2161 aaacggcaat ttaaaattgt tggtttacct acatttttgt atgctcttct tatcccaaat
     2221 gaaatggagg ctcagggatg agaaaagaca tacgcgaagg agtgatgatc tacgtgatca
     2281 atgaaatcaa acccaattat gctgcactag caaaacaata cgactgcgac taccgaacag
     2341 ttaagcatgc ttacgaagaa gcccaagtta aagaatcaaa acctccagaa cgtaagaagc
     2401 gtccaagtaa gttagatccc tacagagaaa tcatccaaga caaaatcaaa gaccaatgca
     2461 gtgcttacag cattttcaag tttattgagc acaaaggatt tggaggaagc tacagtttag
     2521 taaagaccta ttgtcgccag attaagcagg cgaaagtaca caaggcaacc gtccgagtcg
     2581 aacatagccc tgggctttct gctcaggttg actggaagga agatatgcga ctaatcagta
     2641 atcagggcga aatttttgag ttcaatatct ttttgtacgt cttgccatat tccaagaaaa
     2701 aatatgtcac actaactttc gaccgaaaac aagacacact ctttgagtgc ttagatgatg
     2761 cgttttatca aaccggtggc gttcccaaag aaatctggtt cgataatatg aagacagtcg
     2821 ttgaccattc tcgtagtcag ttttccaaac cgcatttcaa caaacgtttt tatgctttca
     2881 gtaaggatgc cggcttcaca ccaattgcct gtcggccatt tcgtccgcaa acaaagggct
     2941 gtgtcgaagc tttagccaga actgttgacc gcctacgggt ctataatcat gaattttatg
     3001 atgccgttga gctgtgtcat ttagtgaata acctttgtgt tgagttaaat aacgaggttt
     3061 ctcaatcaac tgatcaaaca ccagatgaac gttggcagac gaacgaaaaa gagcacctgc
     3121 atccatacca aaaaaacctt ttgaatccct attttgaaga ctttattcgc cgtaaagtca
     3181 ccaaggaaga aatggtcatt ttccgtaaat gcaggtactc tgtggcgcct cgctatatcg
     3241 gtaaagaagt cgaaatcgaa ctctccgata aagaagacca agtacaaatc tattataacg
     3301 gagaactgat tcggtcgcat ccattaacga caaaacagct gaactaccac gaggaagact
     3361 atttccaaat cttgaaatcc gatgtcatga gtcacaaaga agacgatgga atcagagcgt
     3421 atactcgtga aagtctcaag caatatgatc tggtggaggt tgccgatgaa gaatagccat
     3481 taccaagaac tgatgaccaa cttagagact cttggtttga atcatatgcg ggaatatgtt
     3541 cctaactaca tcgaagtggc caatcgagag gagctttctt taacggaagc tcttctcgag
     3601 ctgacacgca gagaagttga tcatcaaaat aagcaaaaaa tgaatcgtgt ggtggcacgc
     3661 gcccgctttt ccaagagaac cagcctggaa gagtttgatt ttgccttcca gccaagtatc
     3721 aataagcgcg aagtgctaga cttaaggcac atgggctttg tggaaaaaca tgagaacttg
     3781 gtttttatcg gcagtcctgg tgtaggcaag acacatctga cgattgcatt gggaatcgaa
     3841 gcctgtcgtc aaggctatcg gacactattc atcacctgtc atgagctgct tttgcgtctg
     3901 cgaacagcct atgaaaaagg taccctagag cgtgttctca aacgatacgc tcgctatgac
     3961 gtcttgatta ttgatgagat tggctatctt ccgattcaaa aacaggaagc tgatttattc
     4021 tttcagcttc ttgccatgcg ctatgaacac cgctctacga tcattacgac gaacattgtc
     4081 ttatctcgtt ggggggagtt gttccaaagt tctgagatcg cagctgccat ccttgaccgt
     4141 ttagtacatc acgtcaaggt gttcaagatc acaggcaagt cttatcgtat gagagacaag
     4201 atctaaagtg tcgattgtcg acaattacta ttgccgaaaa cctacatttt tatcctgccc
     4261 aaaacctaca attttaagct gcccttgaca tcaaaaagac acttcacaag ctaagtgcta
     4321 tcgacaattg aaacctttgt ataaattact tctataatca gatgacgagt tggattatac
     4381 aacattcaag aaatggcaga attttcaatg gcgctattta acagaaagtg aagtagttga
     4441 ttgtctaccc tcaatctctt atgaacttaa aatggcttag cagtattatc aaaagctgct
     4501 taacaattat cacaagaa
//
LOCUS       DAKBVG010000184         4489 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000184 DAKBVG010000000
VERSION     DAKBVG010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.184"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            <1..308
                     /locus_tag="OUD09_002282"
     CDS             <1..308
                     /locus_tag="OUD09_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCT9693517.1"
                     /translation="RHGNQKLSDEITRVGSSPIRKLGPNDRLIAPIVKLDSYHLPYDA
                     GIKVIAAGYHYRNLADPEAEQLRRMIVNDGLKATIKKISRLEGKLLNEVVAAYESQI"
     gene            320..751
                     /locus_tag="OUD09_002283"
     CDS             320..751
                     /locus_tag="OUD09_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCT9693518.1"
                     /translation="MLKEQLSGNTMFMDAIDSWQEAIRVSARPLIGKGIIEESYVQAM
                     IQNVLDNGNYIILLPLVAMPHARPEFGSKGVGMSFLCLDKPVMFPSEEPVKFFFTFSS
                     DSPKGHLDLMANLGELLSDEKMYQKLFDVKTEKALLDLIKK"
     gene            765..2210
                     /locus_tag="OUD09_002284"
     CDS             765..2210
                     /locus_tag="OUD09_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HCT9693519.1"
                     /translation="MEVANKKKRVSMRAKVQAIGGFLTNMVLPNIGAFIAWGIVTALF
                     IPTGWFPNESLNELVSPTVSYLLPLLLAYTGGRMVGGTRGAVLGTIGTIGLIIGADIP
                     MFMGAMIMGPLGGWMMKKTDDFLDGKVPAGFEMVVNNFSIGIIGFILMCLSYLVVGPI
                     IQSANQFVTSAIEALVSTGFLPILSLINEPAKVLFLNNVIDQGVYYPLGMQQTLEAGR
                     SIYFMVASNPGPGLGLLLAYSLFGKKAAKQTAPGAIIIHFFGGIHEIYFPYVLMKPLT
                     ILSMIAGGMTGIITFELFDAGLVAGPSPGSIFSYLALTPRGNFLGVMAGVVTATLVSF
                     FVTSVILKADKSTDEDRNFELSVQKSKTMKSEGRKLLKEENAREICTEKKEMNKISFA
                     CDAGMGSSAMGATIFKKKLQKAGITDVEIKNYRIEEVPEDSDLVIVHQDLEERTRKAH
                     PNMKIVTLKNYLQDPVINGLAEEIIGLKTED"
     gene            2496..4121
                     /locus_tag="OUD09_002285"
     CDS             2496..4121
                     /locus_tag="OUD09_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCT9693520.1"
                     /translation="MKKIYSKEIDCFRGIAVLAVIFYHLPNSLLPGGLQGVTIFFVVS
                     GFLMAKNTIRDEKNRQFSFLHFYYKRIKRIYPFLLFSLVVFIFILGISNIRLIGNIRS
                     ELPSLIFGYNNWWQLNQGVSYFDSYFNASLFKHYWSLSVELQFYLIWPFLFIVIKRMR
                     RKQVFYLIYTLIFVSILFSLFLPSTKAYYHTIAKLFPFLLGVWGYFNRITIGRFFEQN
                     TFSKIWLLLLASLCLILFPIFPYTLNELLISICFALLLASVDDMNIAKKTKIASTKLS
                     FVGKISYELYLVHFPVLIALTNLFKHHHWKYLDIAGLFTVFLCTLLMITCFRKGVQKM
                     RSPKIWTAMALMIIGVSVLVLRAPASRLTADQNQLQSLLKENSSKANAGENLLSQEKK
                     ILFIGDSVMLGAYQELKETFKKEALVDAKESRQITALPEILKEHKDLVSYQQIVIGLG
                     TNGVITDEAIEQTMSLLKDKKVYWVNIKAPTGWQDTINATLATLPNQYSNITMIDWYS
                     ESKNHPEFFYDDETHLNESGRTAYAKYIASVIQ"
BASE COUNT         1498 a          658 c          916 g         1417 t
ORIGIN      
        1 accgtcatgg gaatcagaaa ctctccgatg aaattactcg tgtaggaagt tcccctattc
       61 gaaaattagg ccctaatgat cgtttaattg ctcccatagt aaaactagat tcatatcatt
      121 taccttacga tgcaggaata aaagtaattg ctgcgggata tcattatcgg aatcttgctg
      181 atccagaagc tgaacaacta cgtcgaatga ttgtaaatga tgggttgaaa gcaacgatta
      241 aaaagatttc tcgtttggaa ggtaagctgc tgaatgaagt tgtcgctgct tatgagtccc
      301 agatttagag gaggagaata tgctaaaaga acaattgtca ggaaatacaa tgtttatgga
      361 tgcgatcgat tcatggcaag aggcaatccg agtaagtgcg cgtcctttga taggaaaggg
      421 aataatagag gaaagctatg tacaagcgat gatacaaaat gtgttggata acggaaatta
      481 tattatatta ctgccactag tagcaatgcc tcatgcgcgt ccagaatttg ggagcaaagg
      541 ggtcgggatg agctttttat gtttagataa gccagtcatg ttcccaagtg aagaaccagt
      601 aaaattcttc tttacctttt cttctgattc gccaaaggga catttagact taatggccaa
      661 tcttggagaa ttgctgagtg atgaaaaaat gtatcaaaaa ttattcgatg tgaagacaga
      721 aaaagcattg ttagatttga taaagaagta aaggagagaa gaaaatggag gtagcaaaca
      781 aaaaaaaacg agtctccatg cgagctaaag ttcaagcgat tggcggtttc ttaacaaata
      841 tggtattgcc aaatataggc gcatttattg catggggaat tgttactgca ttattcattc
      901 caactgggtg gttcccaaat gaatctctga atgaactcgt aagtccgaca gtcagctatc
      961 tattgccgtt actacttgcc tatactggtg gtcggatggt tggtggtaca cgtggagctg
     1021 ttttaggtac aatcggaaca ataggtttga ttattggtgc tgacatacca atgttcatgg
     1081 gagcaatgat tatggggcca cttggtgggt ggatgatgaa aaaaacagat gattttttag
     1141 atgggaaagt tcctgctggt tttgaaatgg tcgtcaataa tttttctata ggaattattg
     1201 gtttcatact gatgtgcctt tcttatctgg tagttggacc aataatccaa tcagctaatc
     1261 aatttgtaac gagtgcaatt gaagcattag tttcaactgg ttttttaccg atactgtcct
     1321 taatcaacga accggcaaaa gtcttatttt taaataatgt tattgaccaa ggtgtttatt
     1381 atccattagg tatgcaacaa acattagagg caggcagatc aatttatttc atggtagcat
     1441 caaatcctgg ccctggctta ggactattgt tagcttattc tttatttggt aaaaaggcag
     1501 caaaacagac agcaccaggt gcaatcatca tccatttttt cggcggaata catgagattt
     1561 attttccata tgtattaatg aaaccactca ctatattatc tatgattgct ggtggaatga
     1621 caggaatcat cacttttgaa ttatttgatg cgggcctggt agcaggtcca agtccaggtt
     1681 ctattttttc ttatttagct ctaacgcctc ggggaaattt tttgggtgtc atggcgggag
     1741 tggtcacagc aacactggtt tccttttttg taacatcagt aattttaaaa gcagataaat
     1801 caacagatga agacagaaat tttgaactat cagttcaaaa atcgaaaacg atgaaatcag
     1861 aaggaagaaa attattaaaa gaagaaaatg ctagagaaat atgtaccgag aaaaaagaga
     1921 tgaataaaat ttcctttgct tgtgatgcag gaatgggaag cagcgcaatg ggcgcaacaa
     1981 tattcaagaa aaaattgcaa aaagcaggta ttactgatgt tgagataaaa aattatcgaa
     2041 ttgaagaggt accagaagat agtgatttgg tgatcgtaca tcaagatttg gaggaacgga
     2101 ctagaaaagc acatcctaac atgaaaattg tgacattaaa gaattatttg caagatccag
     2161 taatcaatgg attagcagaa gaaatcattg gattaaaaac agaagactaa tttatgggaa
     2221 tttttgatta acctttatta aaattatttt gcctaatata aaaaggttgt gacatggctt
     2281 gtgtcgcaac ctttttttgt cgcacaaata attaatcgga tattttatgt tcatattttt
     2341 tgtgatcaac gagaactgat ggggcaaaga tacattttag atacttttaa ctgttaattt
     2401 acaaaacaag gtgaaacgaa tagaaaaatg aaaaaatcga tacggaagaa tatgtttgat
     2461 taaaacatgc atgaagagaa ggaggttgat gatacatgaa aaaaatatat tcaaaggaaa
     2521 tcgattgttt tagaggcatt gctgttttag cagtgatatt ttatcatttg ccaaatagtt
     2581 tgctgccagg cggattgcaa ggagtaacga tcttttttgt ggtttctggt tttttaatgg
     2641 ctaaaaatac gataagagat gaaaaaaaca gacaattcag ttttttacat ttttactata
     2701 agcgtatcaa acgtatttat ccatttttac tgttttcttt ggtcgtattc attttcatac
     2761 tagggattag caacattcga ttgataggaa acattcgtag tgagctaccc agtttgattt
     2821 ttggctataa caattggtgg cagctgaatc aaggagtatc ttattttgac tcttacttta
     2881 atgcttcttt atttaaacat tattggtcgc tttcagttga actacaattc tatttgatat
     2941 ggccattttt gtttattgtc attaagcgta tgagaagaaa acaagtgttt tacttaatct
     3001 atacattgat ttttgtttct attcttttta gtttgttttt accttcaact aaggcatatt
     3061 atcatacgat agctaaactt tttccatttt tattaggagt ttgggggtat tttaatcgaa
     3121 taactattgg ccgtttcttt gaacaaaaca cttttagtaa aatctggctt ttattactag
     3181 ctagtctttg tctgattctg ttcccgattt ttccttatac actaaatgaa ctactgatca
     3241 gtatttgttt tgctctattg ttagccagtg tcgatgatat gaacattgca aaaaagacca
     3301 aaatcgctag tacaaaactt tcttttgtag gaaaaatcag ttatgaactt tatttggtgc
     3361 atttcccagt attgattgct ctgacaaatc tattcaaaca tcaccattgg aaatatttgg
     3421 atatcgcagg tctattcact gtgtttttat gtacattact catgattact tgctttagaa
     3481 aaggagtgca aaagatgaga tcacccaaaa tttggacagc tatggcacta atgataatcg
     3541 gagtttctgt actggtatta cgtgctcctg ccagtcgact gaccgctgac caaaatcagt
     3601 tgcaaagttt attaaaagaa aactctagta aagcaaatgc aggagaaaat ctgttgtcac
     3661 aagagaagaa aatattattt atcggtgatt ctgttatgct gggggcgtat caggaactaa
     3721 aggagacatt taaaaaagaa gcattggtag atgcaaaaga atcaagacaa atcactgcgt
     3781 taccagaaat cttaaaggag cataaagatt tagtttctta ccaacaaata gtgatcgggt
     3841 taggaaccaa tggtgtgata acagatgaag ctatagaaca aacaatgtct ttgctgaaag
     3901 ataaaaaagt atactgggta aatataaaag caccaactgg ctggcaagat acgataaatg
     3961 ccacattagc tacattgcca aatcagtatt ctaatataac gatgatcgat tggtatagtg
     4021 agagcaaaaa tcatcctgaa tttttttatg atgatgaaac ccatttgaat gaaagtggac
     4081 gtacagctta tgcaaagtat attgcttcag ttattcaata aggtagtgat tacgtgtgtt
     4141 aaccttactt tttcctaata ggtgaatcaa tttaaataaa aaatacttca tatgataaac
     4201 ggtgtttaag aggaggagct ttttaatatt cgtggtaaaa aagagatggg cgaggtcgtt
     4261 aactgagagc ttcttggaaa gaaatcgctt tggatttatc ctctttgtta tacagaacat
     4321 gagcgagacg acaatactag gatgtatgtt ctgatcgaag ttttatggtt acaagcagca
     4381 atcagcttag ataaaattaa ggatcgtagg aaccaagtaa agaaggctgt tagtaaagaa
     4441 acaaacgatc aagtatgttt tactcttaag aagatgcagt gaataaata
//
LOCUS       DAKBVG010000185         4482 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000185 DAKBVG010000000
VERSION     DAKBVG010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.185"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(<1..1180)
                     /locus_tag="OUD09_002286"
     CDS             complement(<1..1180)
                     /locus_tag="OUD09_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCT9693521.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     D"
     gene            complement(1197..2243)
                     /locus_tag="OUD09_002287"
     CDS             complement(1197..2243)
                     /locus_tag="OUD09_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCT9693522.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYSIRVPIQAISNGIDLEKYRPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(2407..4134)
                     /gene="ptsP"
                     /locus_tag="OUD09_002288"
     CDS             complement(2407..4134)
                     /gene="ptsP"
                     /locus_tag="OUD09_002288"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCT9693523.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            complement(4134..4400)
                     /locus_tag="OUD09_002289"
     CDS             complement(4134..4400)
                     /locus_tag="OUD09_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HCT9693524.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
BASE COUNT         1221 a          968 c          769 g         1524 t
ORIGIN      
        1 agtcttcttc ttcttttctt cgcatctctt cttcatgata aacaaccatg tcttgataaa
       61 attcgatcat agcttcgccg aaatgtgtag atgaaatatc ataaagttta gcttctaaaa
      121 cttttggatc tttaggaact tcactggaaa tataatcaat aagtgctggt gcaaagtctt
      181 cgtcggtttc aaaagttttc cctaatgaag gatgatcaaa caatcggttc aaataagcat
      241 ttccttccac gacacaaggt gttccggctg ccatcgcttc tgtataagta agcccttgtg
      301 tttctgaagt agaagcactg acaaaataat ctgccgcttt ataataaagt gctacatcat
      361 tgttgtccac ttctcctgtg aactgcacaa attcttctaa ctgcagttct gagatcaatt
      421 cctctaactc ttctacataa ggtccttttc ctacgataac taatctggcg ttaggataag
      481 cggagatgac ctgaggaaac ccattaatca aggcttggat atttttttca taagaaatcc
      541 tgcttaaaga aagaatcatc aaatggtttt cctgaagacc tagtgactcc cttaatttgc
      601 tgatatcctg ctgagtgata tcagaacgtt tgaatttttc aatgtcgata ccagtaggaa
      661 tcacgcgaag cggtgctatt acaccatagc tacgtaatgt atcaataaca cgttcacttg
      721 gacaaactac acctgtcgta tgatttgcaa ataatctaga caagtatttt acatgggatt
      781 gcctgatcac ttttccttta ccgatataat gaaggtagtc ttcatacatc gtatggtaag
      841 tatggatcag aggtattttc aacttcttag ctaccatctt cccaagtatc cctgctccga
      901 attctgtatg ggtatgaata agatctagtt ctagttcttt ggcaaccaaa taagcatacc
      961 acatgcctct aacaacaact cttcgatcct tgaaagaaat aaatggtaca cttggcattc
     1021 gaatcacatc tttttccaac ttatccgcat taggatcggt cgttgtaaaa atatagactt
     1081 catgcccatg tctttctaat tcttgtttta atgttttgat cgatgtagca actccgctga
     1141 cttgcggaaa gtaggtatcg gtaaaaaagc cgattttcat gtttttcgcc tccaaactag
     1201 tccttgacct gatttacttt ttttaatttg ctagagactt tttcattcag tacactttcg
     1261 tatacatttt taagttcttc accgatttta ttgatacttc gctcttttgc tgtttgataa
     1321 ccggcttgtg tcgtactcgg tagttcttga ttggtaatct gttggatcag tcggataaat
     1381 tcttcgtttg tcttccccat atagcagttc ttgctatctt ctaaccagcc attatacact
     1441 ggaatatctc gaacaagtac gttttgtttg cttgctaaag cttctaaaac aacgatccct
     1501 tctgtctctt cataagaagg gaaaaagaat agatctgcac ctgaatacgc accttctatg
     1561 atctctccac gaatgtaacc aggaaacagg acattttcag gatgatcttc ttttacaatt
     1621 ttccggatgt tccgaggaat cgagtacatg ggaacatgac caaaccaaat aaatgtgtat
     1681 tcgggcatct tcttagcaat ttctacgaaa tcaatgattc ctttacgttc gaaaaacaaa
     1741 ccgacacaaa tgattacttt ttgatccgga gacagtttga aatattctct gaacttttgt
     1801 tctttcattg gatcagggcg atatttctct aagtcgatcc cattggaaat agcttgaatc
     1861 ggcacacgga tactatagct ttccaacaac tgttttgaat aaggtgttgg cgtgatcaga
     1921 tagtccgctt tttggtacaa tcctaccaga tattttttta cgataggaga aagctggttc
     1981 gatccgataa aggaatttcg gaaatcttcc tctgtagaat gcgcatgata gacaactttt
     2041 tttccttctc ttctggcttt attgaccata ttatgactat tgattccgta tgtattgatg
     2101 tgcaggatgt cataatcttc tttttcatct agtgtataag agataccgac ttccgtcaga
     2161 gcacgttttt gatgatcaag cgcacgcccg attccagatt ttgcaagcat cttttcactt
     2221 tcaaaatata ataatacctt caccttacgt cccccttcac ttatatagta taacatagcc
     2281 tacaaactgt cactcaaact taagccctat ttttatctat tcttactgac aaaaacgttt
     2341 tcttaaaaaa gaacaaaaaa actggcgtag ctcttttaca agccacacca gcttttgata
     2401 cgttgtttat tttacatatt catgaacaag ttcaacgact tcttccatcg tatcgcattc
     2461 tttcaatgca cgatcagcaa gttcagccat tttcgctgtg tccaagcgtt tcattaaact
     2521 acgcgtttta agaatagatg tcgcactcat tgagaactca tctagaccca tacctactag
     2581 caatggaaca gctgtttgat ctccagccat ttctccacac atacctgccc attttccttc
     2641 agcgtgtgct gcatcaatta catttttgat caaacgtaaa attgatgggt tatatggttg
     2701 gtacaagtaa gaaacgcgtt cgttcatacg gtctgctgcc attgtgtatt gaatcaaatc
     2761 gttcgtacct acagagaaga aatctacttc ttttgcaaac ttgtctgcta agactgctgc
     2821 tgctggaatc tcgatcatga tgccgacttg gatcgtatca gatacttcct tgccttcact
     2881 gatcaatttt tgtttttctt cttcaaagat cgcttttgct gcgcggaatt ctttgagtgt
     2941 agctaccata gggaacatga tacgtaagtt accatgaaca gatgcacgaa gcaatgcacg
     3001 catttgtgta cggaacattt cgtcgccttg ttcagataaa ctgatacgca atgcacgata
     3061 tcctaagaat ggattcattt catgaggtaa ttgcagatat ggtagttctt tatctccacc
     3121 gatgtccatt gtacgaacaa ctacaggttt tccttccata ccttctaata cagctttgta
     3181 agcttcatat tggtcatctt ctgttgggaa gtctggagaa tccatataca ggaattcagt
     3241 acggtacaga ccaactgctt ctccaccatt attatggaca ccaactagat cttttggtgt
     3301 tccgatgttt gcagctaatt caaaatgctt gccgtcagct gtgacagttt ctgcattttt
     3361 taatttttcc cattctgctt tttgtgctgc gtaatctgca cctgcttttt caaattcagc
     3421 tttttgttca tctgtaggat cgatgattac atcgccttcg atcccgttga cagccaaaat
     3481 atcgccttct ttgactttag cagtaatttc ttttgtacca acgattgctg gaatttctaa
     3541 agaacgtgcc atgatagcag aatgtgaagt acgtccacca atgtctgtta caaacgcttt
     3601 tacaaaattg cgatctaatt gtgctgtatc acttggagtc aagtcatgtg caacaacgac
     3661 cacttcttcg ttgatcattg atggatttgg taaagtaacg cctaataagt gcgctaaaat
     3721 acgtttagca acatcacgaa tatctgcagc acgctcttgc atataagcat tatcttccat
     3781 tgcttcgaac ataccgatat acatatccgt tacttctttt agcgctgatt cagcatttac
     3841 gctgttgtct ttaatatttt gcttgatttg acctaccatt tctggatctg aaagaaccat
     3901 caaatgggca tcaaatactt gcgcttctgc ttcacctaag ctttgcgctg ctttttcacg
     3961 aatttgctgc aattcttcag tagatttagc taatgcagca tccaaacgag cttcttcagc
     4021 cgcagtatct tctactgttg ccttactgaa tgttaaatcc ggttgaacta gcaggtaagc
     4081 ttttgcaacg gctacaccgt cactagcggc aatccctttt agcatttcaa ccattattca
     4141 gataagcctt ctttcttcat tgtttcaacg attgctgcca ttgcatcagc ttcgtcagca
     4201 ccttctgcag taatagtaac atcagaacct tgacctacac ctaaagacat aacgcccatg
     4261 attgatttca agtttactga tttaccttta tactctaagt ttacatcaga gttaaatttg
     4321 cttgctgttt gtactaatag tgtagctgga cgagcgtgga tcccagtttc tgctactacg
     4381 tgaaattctt tcttttccat attaatcggt ctcctttaag tgagtgttgt ttttatgtga
     4441 gggttttctt ttttagtaat tcagagaatc ctaacaaatg cc
//
LOCUS       DAKBVG010000186         4472 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000186 DAKBVG010000000
VERSION     DAKBVG010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4472
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.186"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(351..611)
                     /locus_tag="OUD09_002290"
     CDS             complement(351..611)
                     /locus_tag="OUD09_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693525.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            686..889
                     /locus_tag="OUD09_002291"
     CDS             686..889
                     /locus_tag="OUD09_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HCT9693526.1"
                     /translation="MIKTVKFVTVAIRLKVRWCVCAYFQNLTERRSLLPVETALGLMI
                     SFGSLIATIIFGILAVVNNNQKK"
     gene            complement(1030..1515)
                     /gene="ssb"
                     /locus_tag="OUD09_002292"
     CDS             complement(1030..1515)
                     /gene="ssb"
                     /locus_tag="OUD09_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCT9693527.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSSSPSPTTFEQRDVATPNNNGLSASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(1518..2417)
                     /locus_tag="OUD09_002293"
     CDS             complement(1518..2417)
                     /locus_tag="OUD09_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HCT9693528.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            complement(2430..3023)
                     /locus_tag="OUD09_002294"
     CDS             complement(2430..3023)
                     /locus_tag="OUD09_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HCT9693529.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            complement(3099..3248)
                     /locus_tag="OUD09_002295"
     CDS             complement(3099..3248)
                     /locus_tag="OUD09_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693530.1"
                     /translation="MRNTTHKQQEIENQKAKIRLEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            complement(3264..>4472)
                     /locus_tag="OUD09_002296"
     CDS             complement(3264..>4472)
                     /locus_tag="OUD09_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HCT9693531.1"
                     /translation="EGEYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHY
                     AIIPTKTVMTAAAFEQLSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKT
                     VGKVPVDLGWKKLFGKESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEG
                     TLITAMKTAGKTVDSEEAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTN
                     KGILLCQAVEKEPLLTSAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPG
                     QIQSTDFGSTLQEVKAASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWT
                     TIAKKKLTATQLKEIIQNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTN
                     YKKRTRRTTK"
BASE COUNT         1132 a          900 c          781 g         1659 t
ORIGIN      
        1 aaaagcgaca aggacaaggg cgcgcagcgt gccatttacc cttgtccttg tcgctttttg
       61 gtgataaaag gcaacttgtt gccggtctgg caaatcaata aagagatagc gaacaggaac
      121 gaaccttttg gtgagaggaa gttttttcta ttttgtcttg ctttgcaaga caccatatct
      181 tagtgaacgt gcaacgttca cttcctttct tctcgaacgg cggtggcggt tggagtgaga
      241 gaaaggtttt tcaattgaga aggcacttgt gtcttcctaa aaaggtcttt tcctgtttct
      301 atgtaaaaca aaaagagctg caatcctcat ttaggactgc agctcttctt ttatatgccc
      361 atcgatcgcg taaaatgatc ggggtttcta atttgcatcg attcaatgac taattgattc
      421 cgtttattga aatgacctgt gacggcaata ttgtattttc catcatccat aattaacaat
      481 ttatcgataa tttcaatatc tgcaactaca caattaatcg attcatgaat tgtttgtaaa
      541 gtaaagcgaa ccaataatgg ttgtgttttt attactcgga ttttatcaag caaaccaaca
      601 tatttgttca tcacgatcac ctcgctaaaa ttatagcaaa aacagctaaa attgagaatt
      661 tttattcgat tttagatatg ctataatgat caagacagta aagtttgtga cggtggctat
      721 ccgattgaaa gtgaggtggt gcgtatgtgc gtacttccaa aatcttacag aaaggagaag
      781 ccttttgcct gttgaaacgg cgctaggatt aatgattagt ttcggttctt tgatcgcaac
      841 aatcattttt ggaatcttag cagttgtaaa caacaatcaa aaaaaataac cgtcagcttt
      901 agccggtcga cgattatttt tcaaaaataa tattaagctg gtcaccgtct taaacggtgc
      961 tgttagggga gttgttagcg caactcctct tttcgtatcc attataacaa ctttattgat
     1021 cttttacaat cagaatggta aatcatcatc actaatatcg attgattgtc cgccaaatgg
     1081 attttgtgaa gcgctcaatc cattgttgtt aggcgtagct acatcccgtt gttcaaaggt
     1141 ggtagggctt ggactgctgg acgattgatc agcgtgcgca cggctttctg tcgctgcttt
     1201 actttctaat agttggaaat tatcggccac aacttctgtc acatacacac gttgtccttg
     1261 ttggttatcg taggttcggg tttgaatccg tccaacaacg ccgatcaaac tgcccttttt
     1321 agctaatgtc gccatcgttt ctgctggttt gcgccaaatc acacagttaa taaaatcagc
     1381 ttctcgattg ccgttttgat tcgtgaaatt tcggttgaca gctagggtaa aagtcgctac
     1441 tgcagtacca ctcgcggtat aacgtaaatc tggatctttg gttaagcgtc ctactaaagt
     1501 tacattattg atcatcttta tctctccatt tcttgattca tttgttgtga tcttttaaac
     1561 tgccgttttc tttcttgatt ttcaattttg atcacacttt tcattaggtg atcaatttcg
     1621 ttgactgtat ctgtaaatga aatggaattc gcaaacgtgt taactgagcg atatttttcc
     1681 cactgctttt ccaaatagtc atttcctctg atcttatcca tttctctttc ataataaaag
     1741 gacaacggtt ttgtatcccc acgttttgta aatgtatttc gaacactttt gtataactct
     1801 ttcgtatcga tattttctag tttcggaatg gtaaataagt cgtagtaatc ttttgctctc
     1861 gaattatctg tgccaaagct gaacgtagca cttagctttt ctgcaataat ttgttctgtt
     1921 ggataagctg gaatctccac ttttttatct tcaaacatta attggtgata gtgtgtatct
     1981 acttcaggat aaatagaatc tcctgttgtc acgtccattt taaacggtac acgtactttc
     2041 tctaaattag ccaccaattt aatttgaaat ccaggataaa aatcggcttc tcttgtttcc
     2101 ttcattcctt gaatagagaa aacaatgcct tcttttgtgg gttttctagt taaatcatta
     2161 aaaatgctct tcaatttttc ttcagttaat ttgctatttc taaatgtcgt atcgatatca
     2221 attgttgtcc ttctatcgat tccatatttt gatcctaata aaaagccgcc tttcaaaatg
     2281 aagtcatttt tgtactttga ttcagaaatt tgttcaatta gacgatccat cataaaatgc
     2341 cgttgcacta actgtgcatt taatcctgta ctctttgcta aatttcttgt ttttgctgca
     2401 aattgtgtcg gtgtcatctt tttcctccat caatataagc tttgcatgtg gtatttcatt
     2461 tctttttcta catgtagttt tgtcatgtaa tctcttaatt tacttggatc tctcaatggg
     2521 tcttccatgt actcttttaa tgcttctaat ttcatttcat attcaaattc atatctcgga
     2581 ttccaaatat cacaaagtgt acgctcttta tcatacatag taataggatt tccttgaaag
     2641 gaagttccct ctacttttcc tagttcataa acttctttct tcacgaaatg aaattgtaac
     2701 tgctgctctt ttgtaattga acgatcaacg ttatagccat ggggaaaagt taagtgaatg
     2761 aatttcggca tattcgtaga aagctggtaa atatctaaag tcgtttcatg agagaagatt
     2821 cccctggaaa atcgttgctg aagccaaaat aaatcatcta ttccatgacc atcctctaca
     2881 taataaccac gatcaacttt gatcaatttt ccttgattca ccaatctttg tacagtggtt
     2941 gctgcgattc caaatttcct ggcttccttc atagataaat ttccatcgcg aatagccaac
     3001 aattctttca gtttatcttg catttacttc ccctcccttc gttacaaata cactacatca
     3061 aatatatcat agtagtttat ttgtaacaag attaacgtct atcgatctaa ttcgatttct
     3121 ttttgaatgc gctgctggtt ctttaattgt tgcagtcttc gtttgctggc acgcattttt
     3181 tcctgttcta aacgaatttt tgctttttga ttttcgattt cttgttgttt gtgtgttgta
     3241 tttcgcatag gataacccct tcattatttt gtagtgcggc gtgttctttt cttatagttc
     3301 gtttttttct tttcagaaaa ttcaaagcct gttgtaaagt cttcttttaa aacgatcgtt
     3361 gcttcaaacg aacctttctg accttttagt cctctgactg attggcttgt tcggccgttt
     3421 tgaatgatct ctttcagttg tgtggctgtg agtttctttt tggcaatcgt tgtccataat
     3481 ttaaaatcac aggcttcaga cgtacatgcc gccacatttt gataaagcaa gacttcttgt
     3541 tcccgacatt ttggacaaac acctaaatga cgcgttgctt cctgtttttc actggctgct
     3601 ttcacctctt gtaacgttga tccaaaatct gttgactgga tttgtcccgg tacgacttcc
     3661 aacaagtgag agacaaattt ttggatatta gctaagaaag tagcttgtgt tcctttctgc
     3721 tcaccaatct ttaataagta actttcccat ttggcggtca tttcggcact tgttagcagc
     3781 ggctcttttt ccacggcttg gcaaagtaaa atccctttat ttgtcaccac aagcttattt
     3841 ttttctacct tgatatattc cttttgtttc aaagtttcaa taatatttgc tcgtgttgct
     3901 tctgtaccga ttccttctac ttctttcaat atggattgtg cttcttcgct atccacggtt
     3961 tttccagctg ttttcatcgc tgtgatcaac gtaccttcgg tataaggttg tggtggcttc
     4021 gtttcttttt caaggacttg tagatctacc gtcaccgctt ctccttttgt cactttagga
     4081 agtagcggtt cttcttcctt ttcttttctg tcgctctctt tgccaaacag ctttttccaa
     4141 cctagatcta ctggtacttt tccgactgtc tttagctgaa gctgctgaac ttgcgtcaag
     4201 atcgttgttt cttcataggt atacttttcc gcaaacatgg ccaccgttgt ttttaacact
     4261 tgttcataaa tagcctgctg cagtggactt aattgttcaa aagccgctgc cgtcatgacc
     4321 gtctttgtag gaatgatcgc gtaatgttcc tggactttct tattattcac atatcgacta
     4381 ttcggtgtat gaataggagt aggaatggct tcggccttta aaaagtgttt gtattcgtct
     4441 aagtgatcca acagatacgc atactcccct tc
//
LOCUS       DAKBVG010000187         4456 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000187 DAKBVG010000000
VERSION     DAKBVG010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.187"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(<1..904)
                     /locus_tag="OUD09_002297"
     CDS             complement(<1..904)
                     /locus_tag="OUD09_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCT9693532.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKG"
     gene            complement(906..2345)
                     /locus_tag="OUD09_002298"
     CDS             complement(906..2345)
                     /locus_tag="OUD09_002298"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCT9693533.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2530..>4456
                     /locus_tag="OUD09_002299"
     CDS             2530..>4456
                     /locus_tag="OUD09_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HCT9693534.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGT"
BASE COUNT         1403 a          771 c          746 g         1536 t
ORIGIN      
        1 cgcctttaat ttttttcaga agaatatcaa caataagact tgcaatttca tcaattggtt
       61 gtcttattgt tgtcaattgt gggaaaaatt tctcaataaa agaggtacca tcgtatccaa
      121 taatttttaa atcttctggg atattaagtt taagttgatg agcaattttc atagtgagaa
      181 tggctgttaa atcatcagaa acaaaaatac catctggttt gtgatttata atagttgact
      241 ttatttccat ttcacgacgg atagtagaca gattattagg aattttgaaa atttttccat
      301 ttggattttg aaaagaaaaa cctagtgcac gtaattctgt gggagaatcc gtgttatcat
      361 ttcctgtaat cataatggta ttttgacaac catttttttg taaagtctta gctgcaagtt
      421 ttcccccttc aaagttatct gaagaaacaa tggaaacatt aggagctaaa tttctatcaa
      481 aagcaacaat aggtgctctc acacgctcat aatcagcaat tccaagatta tggcttgatg
      541 agattatgcc atcaacttgg ttggcttcaa gcatttcaat gtattctctc tctttagcag
      601 gatcattttg actattgcaa ataatcgttt tatatccatg tttaaataac tctatttcga
      661 gatactcaat gagttcagag taaaagatat tactaatatt aggaaaaata agaccaataa
      721 gctgaggtga ctttccttga agtccacgtg ctaaattatt tggtttataa ccaagagttt
      781 tcatagcttc atgaactttt tgttttgttt tttctgataa atatccctta ttattaatga
      841 cgcgtgaaac ggttgttggg ctaactccag ctaattcagc gacatcagtt aattttgcta
      901 ccattttaat attttaattc aaagtatttt ccagtaggtt cgcctgattt gatttgaata
      961 ccattttggt gattttcagg gaatacacga ctagtaaaca ctttttctcc tttattaata
     1021 aaaatctcaa tgattgagtt atctacaaaa atgttagcag ttgtttcttt agtatcaatt
     1081 gagcattcac gaatcgtacc aaagtcagtg gcatattgaa tgcctgcttt gctgcgatct
     1141 aaaataattt ttccttcttt ggtatctaca atcaagctaa gtccatttcc tttactatca
     1201 gagaaaagaa gtaactccga tttctgattt ggaggaaaag ttagttcaag ctcataagta
     1261 ttatttgttt ctgcttttgc tgtaaattgt tcagaactag cacgtaatga tgtaatagct
     1321 tctactggat attggtaaag tttaccatct tttattgaaa gttcttttac aagactcatt
     1381 gctccctgat aatcatactt atcagttgga taatcgatat caggtagtcc aatccaactg
     1441 acaatcagag cacgaccatc aggagtattg aatccttgag tcgcatacgc ttcaaatcca
     1501 taatcaagat tttgaatttc tgaagcgccc acaagtttta ctttttctat gtcaagcgat
     1561 tgacagatct taaaagtatt cggatagata ttcccataat ttagctcaga tttatccaac
     1621 ccttgaggac aataaatgag aacaggtttt ttgtctacaa atactagatt tggacactca
     1681 atcatgtatt ctgaaccagt gccgccaaaa tcaaggttgc caacttcctc ccatgtttcc
     1741 acgttattgt catccgcttt gtaaagtttg ataaagcctg atttttctaa attttgtgct
     1801 ccaacaatcg catacaattg tccttcataa ttaaacattt gtggatcacg aaaatgatca
     1861 gttacatcag caggtttatg aataagaaca ttctcacctt tcgtaatgtt gtagtcttta
     1921 tccatccatg ctccgatttg aagtgtatca cgttcccaat tttcatttcg aacgtttcct
     1981 gtatagagta agaatagatt cccctctatt tcatatgcag aaccggaata agcgccgtgg
     2041 ctatctcgtt ttgtgtctgg aagaagttct gctcctgtta catgaaaatg aactaaatca
     2101 tctgattcat tgtgtaccca ttctttcaac ccatgagcag ctccaaatgg ccaattttga
     2161 taaaaaagcg tatattttcc gttaaaaaaa gaaaaaccat taggatcgtt taacaatcct
     2221 gttttaggtt cgatatgata gctagcacgc caaggtgatc gttttacatt atccttaatt
     2281 ttatccatct catctttagt ccaatcagaa taagcacgat atcgtatttc tcgaggtaag
     2341 ttcatatttt actccttcaa tttatctttt tacaactagt atggtaaacg ttttccgttg
     2401 aaaaatcaat catttcattt aaaaaaataa actatatgac aaacgtttga catattttta
     2461 agatatgcta aaataaattt gcatttcagt gaaccgtttt cacgtaaaaa taaaaaagga
     2521 gtgattcgaa tgaattacag taaagtagct tccgacgtaa tacaagcggt cggaaaagat
     2581 aaccttattg cagctgcaca ctgcgctaca cgtcttcgtc tcgtactaaa agatgacacg
     2641 aaagtgaatc aaaaagcttt agacgaaaat ccagatgtga aaagcacatt taaaattaat
     2701 ggacaatatc aagtgattat cggtgcaggt gatgttaatt tcgtttatga cgaacttatc
     2761 aaaaaaactg gactttcgga aatttcaaca gatgatttaa aacaaattgc tagtaaagat
     2821 aaaaaattca accccattat ggcattaatt aaattattat cagatatttt tgttccaatt
     2881 atacctgccc ttgttgcagg tggtttatta atggcattac aaaacttttt aacagctgct
     2941 ggactttttg gaccaaaatc aattgaagaa atgtatcctg cgattgaagg tatttcctca
     3001 atgatccaac tgatgtccgc tgcaccattc atgttcttac caattttagt aggtatttca
     3061 gctgctaaac gttttggagc aaaccaattt cttggtgctg caattggtat gatcatgaca
     3121 acaccagatc ttggtggctc aactgaatat tggaatattt ttggttatca tgtttcacaa
     3181 acaaactatg cttatcaagt tattccagtt ttagcagctg tttggatatt atctatatta
     3241 gaaaaattct ttcataaaaa attgccatca tctattgact ttacattcac gccattatta
     3301 tcagtaatga ttactggatt tttgactttc acagttattg gccctgtaat gttattgttg
     3361 tctaatggaa ttacagacat tattgtttgg ctatataaca ctactggatt tattggaatg
     3421 ggtatttttg gaggaacata ttcacttata gtcatgactg gtttacatca atcatttcca
     3481 gctattgaaa ctcaattact ttcagcgtgg acaaatggta tcggacatgg agactttatc
     3541 tttgtagttg catctatggc aaacgttgct caaggtgctg caacatttgc catttggttc
     3601 ttaactaaaa attctaagac aaaaagtctt gcttcctctg cgggtgtctc agcacttctt
     3661 ggaattacag agcctgcctt gttcggagtt aacttaaaat accgctttcc attcttttgt
     3721 gctttaattg gttcaggaat tgctacagca gtagcaggat tacttaaagt agttgctgtt
     3781 tcacttggtt cagcaggttt cttgggattt ctttcaatta atgcaacatc tattccattt
     3841 tacgtattgt gtgaattgat tagctttgta gttgcttttg caattactta cttctacggt
     3901 aaaacaaaag ctgctggtat ctttaaagca gaagaaattg ctgaacaaac accagttaat
     3961 tcttcaaaag tagcaacaac agatccaata tctactgatg ttgaatcaac acaaacaaaa
     4021 gtttcatcag aaacaattgt tagtcccatt tctggtaaag tgattagtct ttcttcagta
     4081 aatgatccag tcttttcatc agaagctatg ggaaaaggga ttgccattaa accaaatgga
     4141 aacacggtcc attctccagt agatggtact attcaactca cgtttgaaac tggacatgct
     4201 tatggtatta aatcaaataa tggtgctgaa gttctaattc atattggtat tgatactgta
     4261 tcaatgggag gaaaaggatt tacacaaaaa gttactacta atcaaaaagt tagaaaaggc
     4321 gatattcttg gagcatttga ccgttcaatg attgagaaag aaggtcttga tgatacaaca
     4381 atgattattg tgaccaatac ttcggattat tcagaagtaa atatagtagc tgaaaataca
     4441 gtcactgagg gtactg
//
LOCUS       DAKBVG010000188         4408 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000188 DAKBVG010000000
VERSION     DAKBVG010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.188"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            81..962
                     /locus_tag="OUD09_002300"
     CDS             81..962
                     /locus_tag="OUD09_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT9693535.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            975..2585
                     /locus_tag="OUD09_002301"
     CDS             975..2585
                     /locus_tag="OUD09_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HCT9693536.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2662..3321)
                     /locus_tag="OUD09_002302"
     CDS             complement(2662..3321)
                     /locus_tag="OUD09_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCT9693537.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3604..4269)
                     /locus_tag="OUD09_002303"
     CDS             complement(3604..4269)
                     /locus_tag="OUD09_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HCT9693538.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
BASE COUNT         1184 a          859 c          870 g         1495 t
ORIGIN      
        1 tataaaaagg tataaaacct ttttattaaa ctatttaatc ttgtttagta tatattttta
       61 gaaaaaggag tggggttttt atggcagaaa tcgtcaaagt cacacatttg aaaaagagct
      121 ttggtcgttt cgaagcttta aaagatgtct ctttcaccgt acattcaggt gaagtcgttg
      181 gatttatcgg accaaatggt tctggaaaat ccacgacgat tcggacactt ttagggatta
      241 taaaaaaaga cggcggaacg gcaacgattt ttgggcaaga tgtatgggaa gatagtttat
      301 ctatccatga acgtctttct tatgttccag gcgatatctc tttgtgggga aatttgactg
      361 gaggagcgat catcgatctc ttcatgaagc ttcatggaaa agggagtgct caaaaaaaag
      421 atgaactgat tcaacgtttc tctcttgatc ctaagaaaaa agcaaaaaac tattcaaaag
      481 gaaatcgcca aaaagtgggc ttgattgctg ctttggctgt tgattcggat ctctatttat
      541 ttgatgagcc tacttctggt ttagatcctt tgatggaaac gattttccaa gaagaagttg
      601 aaaaattgaa agaacagggc aaatccatcc tactttcttc tcatattctc agtgaagtcg
      661 agcgactagc ggataaagtc gtgatcatac gcgaaggaac actcgatgaa gaaggaacac
      721 tcgatgaatt gcgtcatttg actcgttcga ccatcactat agaaacagaa gcagagaata
      781 gtcacttagc acaattgaac ggtgtccatg actttgtgca aaaaggtcac ctcgctcaat
      841 ttgctgctga taacgaagct ttcgatgtga ttttagctga agcgtccaaa ttaggtgtga
      901 aaaaatttga atctattcca ccgactcttg aggatttatt catgagacat tatgaaggat
      961 gaggaggaag cgaaatgact gataaatttt cacgctggtc cacccttttt tctcaaaacc
     1021 ttagccgtga ctggaaaaag atcctgatat ggctagtcgg tgtcactgct ttttctggtg
     1081 gttttgtccc tgcgtttgaa gaattgactt ccggacaagg attagctgga ttatacgaaa
     1141 ccttgcaaaa cccggcaatg attgcaatgg tcggaccaac tccagcttcg aatgctgctg
     1201 agtatacttt aggagcgatg tatgcaaatg aaatgttgct tttttgcagt ttgtttgctg
     1261 ctgtgatctc tgttttacac gtggtgagcc aaacgagaaa aaaagaagaa cttggtctga
     1321 ttgaacttat tcgttccttt cgagtcggac gtcaagcaaa ctctttagct gtcatcgctg
     1381 aaagtattct gatcaatttg cttttagcaa tatcgatcag ctgtacgatg atttttttcc
     1441 aagcggattc gatcactgca gaaggtgctt ggctttttgg gctatctgta ggtagtatgg
     1501 ggattttggg gagtgccgtt gcactgtttg ctgcacaact gatgccaagt tcagccagtt
     1561 cgaatggtct gtctttagcg tttttaggtg gactttacct tctacgagca ataacagatg
     1621 tagctgattc ctccctttcg atgtggaatc ctttaggctg gtcttatttg acctatccat
     1681 ttacaactaa caactggatt cctttgcttt atttgttttt gttcagtcta atcttgataa
     1741 tcagttcatt tgttttagag gaacatcgag atttgaacac cggctatatc cccgaacgtg
     1801 aaggaaaacc gtatgctaaa cgttctcttt tatccttgcg tggtttatta ttctatctaa
     1861 ataaagggat gacaatcagt tggcttatca cttttggttt gatgggggcg gcatacggct
     1921 ctatctacgg gaatatgcaa cagtttttag aaagtaacga cttaatgaaa cagatgttta
     1981 tccaatctgg cgtctcaatt gaaaaaaact ttacaagcac gatcatggtc gtcttgattt
     2041 gtctagctgc tattttacca attgctagca tcaatcgttt atttacagaa gaaacccacg
     2101 gacgtatgaa tcagctgttc gcaacaaaaa tttctaggaa ccaactttat tggaccaata
     2161 tgttactagc tattctattt gctatattgg caatcctgat tagttctggt gcactagggc
     2221 tttctgcttt ggcttcgatg aaggatgcga aagacatgga tctattgatg tttcttaaag
     2281 ccgggttcaa tttcctgcct gccgttttag tgtttatcgg aattagtggc tttctattcg
     2341 gatggcttcc tcgaatcggt aaacttgctt atatctactt agcttactcc tttgcactca
     2401 attactttgg cggaattttg gatttaccag aatggttttc caaaacagct gtccctagct
     2461 gggttccacg gatgccaaac gattcttttg atctttccgt gttccttacg ttatcatgta
     2521 tcagccttgc actgttcgtc cttggttcta ttggttatag gcgaagagac ttactagaag
     2581 gataataaaa aagcatgtac aatttcatgc tagcctccaa atctgggtca aaaatccaac
     2641 atttggaggt cattttttta tttacaagtc ttctaactgc tctttatcaa tgatccagac
     2701 ttctcgattc tttcttttta cataaccatt ctcctctaat aatttaaatt ttctagataa
     2761 agtttcgggg gtcgtaccga taaatgcagc aagttctttc attttcattg gcaggaaaaa
     2821 gtgatccttc cctgtcgctt tagctaagtc aagcagatag acagcgactc gttcttctac
     2881 tttttccatc atcaaaaaag ttgtctgctg ctgtactttt gttaatttct caacgttgat
     2941 tgctaaaagt ttcagactaa gggcgggata atgcagtaag agttccgaga aatctttttg
     3001 tctcaatatg caaatttccg ttttttctag tgcttcgcca aataaagact catttggaac
     3061 gcctagcaac tggctctctc cctcatatcc gcccggctct attactcgta aaagctgttc
     3121 ctttccgcta gcagataatt gatacacttt catactgccg gaggccacga tcactaattg
     3181 aggttctcca ttcggcgcaa gaatctgttc tcctttttcc acatagtgat gtttgacaag
     3241 tgcgtgtact ttttcttgat cttgctgtac taagtgattg aacaaaggaa caaatgaaac
     3301 acataaatga tcatgactca taatctctcc tccttctaaa cagctatttc taataaagaa
     3361 acataacttc ccttattcca actgataatt ttcttttatc atagcataaa cgtcaagcga
     3421 accagatacc agcaaataga gtggatttct ttttgtccct tgctttattt tccttctttt
     3481 gagaccttaa gtaattgatt cctatcaatt ttttaaatat ttactttttg aataatcaaa
     3541 aacaaacctt cactcgcaag ccgagagtaa ggtttgtttt tcgcattgct tatttcgtgt
     3601 tgtctattga aatttttccg cacctagctt ttgatatagt ggtgctagtt cttgtaaggt
     3661 ttcagcggtt atttgctcaa cgtgttcgat ttcttgcgga tagaagaata gtttacccat
     3721 ttgaaaggtt cggtctctca tcgctttttt caactcttct tgtttctttg tatcggacaa
     3781 atagtcagac aaagctaagt ggtcgtcaag ttcatgttct ggttgcggcc atatgtaata
     3841 atccttgata tcgactgttt tgctccactc tttcagtatc gcaagcaact ggttatacgc
     3901 tgcaatgcta ccatatcgtt cttcgctttt ataatgttgg tacatcaaat agatttggta
     3961 ttgtttttga ttcaacaaaa ctgctagaca ggcattctcg tcttgacgag attcgcttcg
     4021 gtacgcgctc caaaaatgac tgcgtaaatt ccagccattc gtccagcttt cgattttagg
     4081 tttgctcatg ccgtacatat caggcaaaag tgcagctgtc tggagctgaa tctctttcca
     4141 tttttgccag tgttctttat attctttctt gatttgttca atcttatctg ttggatggct
     4201 ctctttgagc tgtttgaatg cgtagattgg tcgatcaaac aactgataat ctgattttcc
     4261 aagttccatt gtttcgcctc tttccgttat cttttgtatt ctacctgaat tttgcgatat
     4321 ggacaaatag aggttgtgaa aaagctgttc tgcatcaagt cataagaaca gccaaacaaa
     4381 aaactgcgat gagcatcacc gcagtttt
//
LOCUS       DAKBVG010000189         4296 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000189 DAKBVG010000000
VERSION     DAKBVG010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.189"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            8..919
                     /locus_tag="OUD09_002304"
     CDS             8..919
                     /locus_tag="OUD09_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693539.1"
                     /translation="MGERTFEDVKDYVELQSQEKCTVLSAKPEQEFNDLGTEVTVWNV
                     KTDTDGDWWVVEGDNVPMNLYPQSAYYFGTDEVYSFHMGLMERMRVLHEKYDPEDFVR
                     SVTLDTEIAPQLFRKLKNVATLIDSAEEIEDFQSIGVQCREILIELGNHIFIADMAGT
                     KEQPQKSNFKKKAELFIQFYLTGSDNSDYRSIIKKLTESTWDYANKLTHSSTTTKYEA
                     STCVMLVTSLVGVYENITQKVFDPVSQYICSSCKSKKLTIDSDDSDENGMVESLYLKC
                     EECGDITKVVFLTSDKDSSYTKGKIIE"
     gene            complement(1253..2395)
                     /locus_tag="OUD09_002305"
     CDS             complement(1253..2395)
                     /locus_tag="OUD09_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCT9693540.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(2434..3168)
                     /locus_tag="OUD09_002306"
     CDS             complement(2434..3168)
                     /locus_tag="OUD09_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCT9693541.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            3400..3675
                     /locus_tag="OUD09_002307"
     CDS             3400..3675
                     /locus_tag="OUD09_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCT9693542.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            3773..>4296
                     /locus_tag="OUD09_002308"
     CDS             3773..>4296
                     /locus_tag="OUD09_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693543.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENIEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRM"
BASE COUNT         1377 a          652 c          775 g         1492 t
ORIGIN      
        1 gtaaataatg ggtgaacgaa cttttgaaga tgttaaagac tatgtagaat tgcagagcca
       61 agaaaagtgt acggtgttaa gtgccaagcc ggaacaggaa tttaatgatc ttggtacaga
      121 agtaacggtt tggaatgtca aaacggatac tgacggcgat tggtgggttg ttgagggtga
      181 taacgttcct atgaaccttt atccgcaaag tgcatactat tttggaacag acgaggtata
      241 ttcgtttcat atgggtttaa tggagagaat gagagtttta catgaaaaat atgatccaga
      301 ggactttgta aggagcgtta ctcttgatac agagattgca ccacaactct ttcggaagct
      361 aaagaatgtt gctactctta tagacagtgc agaagaaatt gaagattttc aatctattgg
      421 tgttcagtgt agagaaattc taatcgaact tggtaatcac atctttatag cagatatggc
      481 aggtactaaa gaacaaccac aaaaatcaaa cttcaaaaaa aaggcggaac tttttataca
      541 gttttatttg acaggttccg ataactctga ctatcgaagt attataaaaa aactaactga
      601 atcaacatgg gactacgcta ataaattaac tcattctagt acaactacta agtatgaagc
      661 ctcaacttgc gtaatgcttg taacgtcttt ggttggtgtg tatgaaaaca taacgcaaaa
      721 agtctttgat cctgtctcac agtatatatg ttcgtcgtgt aaaagtaaaa aactcacgat
      781 agattctgat gattctgatg aaaacggaat ggttgaatcc ttgtatttaa aatgtgaaga
      841 atgtggtgat attacaaaag tagtattttt gactagtgat aaggatagtt catacacaaa
      901 aggaaaaata attgagtaat gattatggat catgtcaaaa acatatcacg caattcaaag
      961 tgcacacgtt tttaaatttg atacctcaaa taacttctct acctgctttt ttgggtacga
     1021 tattggatac gttctggggg atatcaagtg ctgtgatagg ctgtcagtga tatataccag
     1081 agcgggaaaa atgcttttat aacgcgaatc atgtaccaat gctattgaaa agagtcattg
     1141 gaaggcagtg ttgggtaaac ctgttatagg agctagaacc cttgatatat aagggttcta
     1201 gcttttttgt tttgcctgta tactagaaat gaccagattt tgaccagctc ttttactgat
     1261 gagccatcat atcagcaaag tcttgacctg ttttcttagg tgcattattt gtaatatgtg
     1321 catagatgtc taatgtcact ttactatttt catgccctaa tcgttgttga actaccttga
     1381 cagattctcc tgcttctaat aataaactag catgtgtatg tctaaaccca tgaggtgtaa
     1441 tacgaggcaa gttatatttt ttcaaaatat atcttaacca atcattaaca gtgtttggaa
     1501 tgtatagagt gtttttaact gttgtaaaaa catgttgctc tttagagcta gtattgtatc
     1561 caagttttaa atactcttct ttttgctgta atttccagtt gttgaggata gaaagagtct
     1621 cagtatctaa agagataact cgtctagaag ctcttgtttt cggagattga attataactt
     1681 taccaaattc atctacagca agagttttat tgacatgtag ttctttatta aacgtgtcta
     1741 tgtctttcca ctgtaaagcg agtacttcac tctttctcat acctgtgtat gccaataatc
     1801 tgaagaaggc gtattgtttc atgttaccaa aatctttaaa agcatccagt aaattattaa
     1861 gctcctcttt tgagtaaaat ttacgatttt tctcatactc tttttttcgt ggaagaagtg
     1921 ttttttccat aggattattg tcaatatatt gcattgcaac cccgtagcgt aaaatttgtg
     1981 cagtctgttt tctcatataa tcgtaacttt catatttact gtgccattca tctaccactt
     2041 tttgacaata tgggacagag atattcgata gtttaagctt accaaatgct ggaagtattt
     2101 gattatttgc gtatcttact gagattgcga ttgttgaagc tttaacactg gtacgctgat
     2161 gagttatcca ttcattatac aggtcttcaa atgtcatatc aggtgctttc tttttaacac
     2221 cgtataatat ttgatgtctg tactcgtcat aatctttttg agcttcttgt ttgctactaa
     2281 aacctcttct cgtagtattt attttttcac cagttcgtgg atcggtggca atgtgtcctt
     2341 tatacatcca cgccacttga ccgtttttct ttttgtactt tgatattttt gccattaaat
     2401 catctcctct tagtttacaa ggagtactta ccactagatg gtatcacctc ccttcatttt
     2461 tttcttagtg tattttttcc caattgatga aatcgtttgt tctagcttgt ttatattatc
     2521 agtaatttct ctcacagctt cttcgggata agacatgtct ttcaattgaa taggtagcca
     2581 ttcatctacc attctttcta gtagttcttc tatactgcta gtgaggaaaa gaatgttatt
     2641 gtcaaattct tctacaatag cattgatgaa taattcttct atttttaaga tattgaaaag
     2701 actaattttt tcatttttaa ctttattaca agtcttgtta gcaataaatt cttgcatctc
     2761 tggttctaat ttggaatatc tatccagcca tgtatctaaa ttagagtgta ctggtgggaa
     2821 ctgttgacga agatgctgga acaggttttt atctggaaat gttataccat ctagtacaat
     2881 gctatctcta taaagaataa ttcccaaaat atattcttct acattgccac aaactaactc
     2941 ctctatactc atatttccca aatcagcaat tcgggataat cgctctggta ttggtaaata
     3001 ggttcccttc tcccattcgt agatagtttt tttgtcaatt aattgaccgt tgttaatcat
     3061 aagaccaaat gctggttgtg ttaatttttt actctttctt atagctttta tttttgttcc
     3121 gagtatcttt tttcgttcta aataatttat tggtggacgt tttatcatat tgaccacacc
     3181 tcctaaaatt agtatacctc aaaaaagggg tgttgtaaat aaattttaca aagctgttga
     3241 cacccctttt ttatcgaggt attatgtgta tgtcgatgaa ctggtaatca ttttttgttt
     3301 gattaatcgg ttaataacaa aatactttta ttttgagcaa aaaatacgaa gataattctt
     3361 agaaaatttg ttcaagcaat cacgaaagga gggggaaata tgtctctaca aatcattgat
     3421 agcattgttt ctatacaagt tgaacaacaa gtgaaagaga taattacagt gctaaagcct
     3481 atgctagaag acattatttc tggagtcact ctagagtata tggactatca gcaggcaagt
     3541 gaatatctag gtgtatcagt aggaacgatt agaaaatatg tttctcaata cgggttacct
     3601 gttataaaaa ttgatacggt cattagattt aaaaaatctg atattgatag tttcatggaa
     3661 cggtataaag gttgagtaaa caaataagac taaaacatat agtaataaag agtttgtttt
     3721 tattatctgt atgttttagt ctttaacaga attttataag gaggaaacac aaatggaaac
     3781 atcaatgatt aatattccaa tttatgtaga tcaagaaaaa ttattagaga agccagttac
     3841 aaaaaaagaa aaaactgatg taagagatag gtgtttagat aactatagag aagttacaag
     3901 agatgagttt gtagagatac taaattcatc acaatcattt attccaagca agacaaagaa
     3961 taaaggcaat gacaaggaga gttttgtgga aaccagagta attattctgg atgtcgataa
     4021 cactgttaaa gatgagaagg ggaaggtagt agatttgagt aaagatgata gtagatactt
     4081 aagcatagaa aaggttttat ctatcaattt agttcataat tctgcgtttg cgatacaaaa
     4141 aagtattcgg tactctgaaa acatagaaaa atacaagatt gtctttctat taaaagaagc
     4201 aattactgac tatggtaaaa tggttgctgt ttacgaatac ttgaaaaaga acatccccgg
     4261 gtgtgatgac aatgtaaatg cttcgaatag aatgtt
//
LOCUS       DAKBVG010000190         4288 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000190 DAKBVG010000000
VERSION     DAKBVG010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4288
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.190"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(176..1462)
                     /locus_tag="OUD09_002309"
     CDS             complement(176..1462)
                     /locus_tag="OUD09_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCT9693544.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(1455..2723)
                     /locus_tag="OUD09_002310"
     CDS             complement(1455..2723)
                     /locus_tag="OUD09_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCT9693545.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            2975..3436
                     /gene="mscL"
                     /locus_tag="OUD09_002311"
     CDS             2975..3436
                     /gene="mscL"
                     /locus_tag="OUD09_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HCT9693546.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(3495..3941)
                     /locus_tag="OUD09_002312"
     CDS             complement(3495..3941)
                     /locus_tag="OUD09_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCT9693547.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(4034..4279)
                     /locus_tag="OUD09_002313"
     CDS             complement(4034..4279)
                     /locus_tag="OUD09_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HCT9693548.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
BASE COUNT         1212 a          875 c          759 g         1442 t
ORIGIN      
        1 gattacttcg tttgatttta ttttaatata agttgattaa aagagagcat gatttgtttt
       61 attttctcac taatttttcc aacacaacta agaataccat aaaagctgat tttggaggag
      121 gttcctacta caagtctaaa acgaaattta agacttttcc tcataaaata ctggtttatt
      181 ttggtctcat gtaaaactcg ctgaaggcct cttcacttat aaatgcttcc ccagctgcca
      241 gtacgtcttt catttggatc gaatcgatga tttcaggaag atcaaataaa gttcgatcgc
      301 caaataaact ttgcgtaaat tgattggcaa tatattcgat cgaatttaat gattggaaat
      361 attgtccaag cattttcttc ttcagcaaat cgagatttgt ttctgacact tccggatcat
      421 cagcgaatcc aagaatgatt tctttgactt tttctgctgc tttctctggt tcgtccgtat
      481 ctccgctgaa gtccgcaaag tgaaattcac gatccaagct gaattcaaaa ccaaagctgt
      541 catcgataat tccttgattg tacatcgcta gatagtttcg agaagtattg cctaaaatca
      601 tttggaacaa cagattgatt gctgttttat agcgtaacag ttctgttcct tcttgcggca
      661 atgtgtctaa tcctttaata ccaagtacaa atttatctcg tgtaatggct gcttccaatg
      721 cactttgttt gatgatttct tttgtgtttt cagggaaata acgtacaatt tcttgctttg
      781 gagggaaatt ttttgcttct tggttttccc gaataagttt catcaatttc tcaggctcca
      841 tcttaccaac tacaaataaa accatattgc ttggctgata aaacgtccga tagcacgtat
      901 acaagtcttg agctgtgatt ttgtcgatac tttcaaccgt gcccgcaata tcgatatgca
      961 atgggtgtgt aggatacaag ttatttagta tgccaaaaaa catccgccag ttagggtcgt
     1021 cttcatacat ttggatttcc tgaccgatga ttcctttttc cttgtttaca gtttcttctg
     1081 tgaagtaagg tgcttgtaca aaatcgatca gagtcgtcag attcttttct acctgatccg
     1141 tcgtagaaaa cagatagctg gttttcgtaa aacttgtgaa agcattggcg gaagctcctt
     1201 gtttcccaaa taattgaaag acatcaccat cttctttttc aaacaactta tgttctaaaa
     1261 aatgggcaat tccatctgga actttcacct tttctttttc accataggga ataaattcgt
     1321 tatcgatcga accataattc gtactgaaca aaccatatgt cttgtgatag tcatttttag
     1381 gcaacaaata cacggtcaaa ccgtttggca gtacttcatg ataaagtgtt tcattgattt
     1441 gctcatattc tgttttattc atttgcattt cctcctgcta aaaagaagat tgcctgcaat
     1501 tcgatttgct cagctacttg ttgtatctct ttgatcgtta cgctctccat acgtttcaac
     1561 cattcttcat cagataattt tgtttccggc agccagctgt ccaaaaaaga agcttcgatt
     1621 gccgcttgcg gagagtctag agaaagcaga tattggtttc tcagcattgc ttttgtctga
     1681 gacagttcca actctgttat ttcaccattt cgcaaacttt ctaattgctc gtgaatcaga
     1741 tgaagtactt gatttcggtt cttttcatcg ataccggttt ggacactcat gaatcctcgg
     1801 aacgtatcca cgctactaga ggcataataa gctagacttt ctttctctct gacattcatg
     1861 aaaagtttag agtgaggaaa accgccaaat aaaccgttaa agaccattag cgcaaaacgt
     1921 tctggttcat cgtaatacac atttgtctga taagctaggt tcagctttgc ttgaacgatt
     1981 ggctcacgta cttgtcgttc cttcacgata ttcacttgag gttgggtata aaacagatct
     2041 ggataaactg tttcccgcga ctcaaatggt aattgcccaa ctgcttcagc tgctttgtcc
     2101 ggatcaacat cgccgacaac aaaaatgtcg atttgatcat ttgccatcat ttcttgatag
     2161 taggacgcta gcgaactagc agtgatcttg tccaaatctt cgactacgcc caaccctggg
     2221 atcttttgtt ccgggctgtt ttcaaaataa agttcttgca aggcatagga agcaaaagtt
     2281 tgtttatctt ccttcaaact ttccaaatat agacgcagat tgttcttttc caagtcaaac
     2341 gtcaactgat caaactgatg atttttaata tttggataaa ataaaacctc ttttaaaaaa
     2401 tctaccgctt gagaaaaaag gtttggatca ttgacatatt caccattcac aaaactgatc
     2461 cctgcgttta gccaatgtaa atttcctttt tttctaactg ataatccaaa gcttgcgcca
     2521 taaagctctg caagttgcgc acttaacttt gtctgatccg gataattcaa actatttgtt
     2581 tccaacatgc tgctgagaag tgaacgtttc gtgatgactt cttgttgcag acgcgcagta
     2641 aaacggataa aaatccggat cgttttgtat tgttttgttg gtaagacatg aagattcacg
     2701 ccttctgcca attgatacac catatttata tgctcctttt cacagttgat tgcccgctta
     2761 ctcttttaat gggtcaacct gcctttttta cttattcctc tgcttgaaat ttataagttc
     2821 aggcaattcg ccgttttttc taaatgatgt tacactttat agtatagcat agatgttttt
     2881 tatcaaaaga aacagacggc taaaagcgta tattgttttt ttatttttta tttttcgata
     2941 tactaaagac aaataaaaga agggagggtc attcatgatc aaagaattca aagagttcat
     3001 catgcgtgga gatgtcttgg atcttgctgt cggcgttgtt attggtagtg cgtttacaag
     3061 catcgtaaac caagtggtgc aaggcttgat cacaccacta ataggttggt ttgtcgcaat
     3121 gatcacaggt acttctgatt tagaaggagc actttcaatt cttgactggt cgccgacaaa
     3181 aggcgtcaca tttgcgtttg gtaacgttat tagtgctatc attacatttc tgatcactgg
     3241 ttttgtctta ttcttagttg tcaaagcggc aaaccgtgcg aagaatttac gctccaaaga
     3301 agagcaagtg gaagtggaag aaactcctac tgcagaagat tatcttagtg acatacgcga
     3361 tcttcttgcc aaacaagtgg cagaagacaa tacacatcca gtagagcaaa caaatctttc
     3421 cgatacttct aaataataaa gaatctaaaa aaaagaaagc cgattttcag tgtcggcttt
     3481 ctttttttag attcttaact tgctacttgt tcctttttca tgtcgatatg tggaatatca
     3541 tcttccaaat acacatctga gattgccttg aatccaaacg actcataaaa agcttgtagg
     3601 tattcttgtg cgccgatcac gattggtttt tctggaaaat ttttctggat catgttaatc
     3661 gtttcttgaa caatctttct tcctaaacca tctttacggt tgtcctgatg aacaaggaca
     3721 cgtccaaaat ggatactctc cgcttcttca tatactcgcg tgtaggcaag gatcttccca
     3781 tcagtatctt gaaaaaacgt atgccaagct tgtccatcaa tgtcatcaac ttcttgataa
     3841 ggacactgtt gttccacaac aaaaactgcg attcgttctt tgacgatcgc gtaaaactct
     3901 gctgttgaca attcttcaaa tttctttacc tgatattcca ctaagcaaac gccccctgct
     3961 tttttattct tagcataccg attcgcacac aaaaaagcaa acgaacctta cgaatctcag
     4021 gctcgtttgc tttttaaact gcttcagtta aaaattcgat ctcaactgtc cgagtaagta
     4081 catctgaata gctgtaagat actcgttcaa aagaattttc ttcaggatcc aaatcaacaa
     4141 cgaatactga aggataggtt tctgttaaaa cgccgcggcg ttctgtttga cgctttcttc
     4201 ctgtctgagc aaccaacatg attgggctgc cgatacgacc ttctaaatct ttcttgattg
     4261 aagctaaagt tgttggcata cagttcac
//
LOCUS       DAKBVG010000191         4262 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000191 DAKBVG010000000
VERSION     DAKBVG010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4262
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.191"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            282..557
                     /locus_tag="OUD09_002314"
     CDS             282..557
                     /locus_tag="OUD09_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693549.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            599..1021
                     /locus_tag="OUD09_002315"
     CDS             599..1021
                     /locus_tag="OUD09_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693550.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVE
                     GKIKSNQTKKYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            1040..2632
                     /locus_tag="OUD09_002316"
     CDS             1040..2632
                     /locus_tag="OUD09_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693551.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            2644..2979
                     /locus_tag="OUD09_002317"
     CDS             2644..2979
                     /locus_tag="OUD09_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693552.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            3126..3320
                     /locus_tag="OUD09_002318"
     CDS             3126..3320
                     /locus_tag="OUD09_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693553.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            3352..>4262
                     /locus_tag="OUD09_002319"
     CDS             3352..>4262
                     /locus_tag="OUD09_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693554.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKEKDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSN"
BASE COUNT         1739 a          608 c          855 g         1060 t
ORIGIN      
        1 aaggattttt tattgtctaa atacacaaat aaccttaatt tttaaagagc ataaggaaac
       61 gtgatatcac tttctcacga aaggtgttag gtaggctgtg cctaccacgt ttctaagaaa
      121 gataaatgat atcaaaattt tggatttgtg tgtagatttg tgggtaggat gaaagcgaat
      181 gtgggcagta aaataagcgt ttgtgtgtag caaaatcaaa aaatgtgtag ggatatgtgt
      241 agctacacat atccctacac ataataaatt tggaggaaaa tatggactta ttaattagaa
      301 atattgaaga aaagtatatt aataaaattg acaaacgttg tgaagaactt acggtcaaga
      361 ctggcaaaaa atggagtcga aatcaatacc taaaagtttt gatcgaaaat gattttgatc
      421 acgctcttct gaattataaa aaagaccaat ttgatcggtt gttagaaaaa tttgtggata
      481 ttcaaactta taatacgaaa actttagaag aatatattgc aacaaataat caattaattg
      541 gtttgttaat tgaataataa aaatagtatt gtgaatttat aaaaggagga aatcaatttt
      601 ggcaaaagca acaagaaaac atgtatatct ttacgatatg catatcgaag tacttgatga
      661 aataatttct actcatgaag atatatctaa ttatagtgaa gcagtcagac atttgtgtat
      721 gatgtatgat ccaaaaatcg aagaaaaaat aaatccaatt caaattaaat tgaatgcaat
      781 gggaaaagaa atttctattt taaccgaaat ggtttcagag tttgcggatc ttcatttgaa
      841 agatacagga atactaattg gtacaaattc aactgtttat caagaagcag aagaacgtgt
      901 ggaaggaaaa attaaatcca atcaaaccaa aaaatattct tctaagttaa agaaaccagt
      961 gaatgaatct gttcatacca gagtagatcc atttgatcca acgaagattc taaaaagata
     1021 aaagtagaag gagaacgtga tgaataaatc gcctgcagtt attcttactt gtcaatttac
     1081 cttgcccaat aaaaaatctt tttcaacata cattgattat atgacaagag aaaaagcact
     1141 ggaagaaatg gaacgacgta cgcccgaaga agaacaggaa ctgcaacgaa tcaagaatgc
     1201 tttagaggat ttttatattc ctgaaggaga aactttttcg gaaagtaaga cggataaaga
     1261 attcagtgaa acgtcgaatg aagctcaatc gttgatggaa catggaatca attttgacaa
     1321 gttagaggaa caagatttta ccaaatatct ttcttatatg acccgtcact atgcgttaga
     1381 acaaaagaaa gaactgacgc aaagggaaca aaaagaatac caacggttga accaagcaat
     1441 gaaaaaatat caaacagagc cacataaaaa agcttcagga aatcttcctg gagttttttc
     1501 tatggcttca gacgaagtga aaggaagtga tctgaaggag atcaaacaaa tcttccaaag
     1561 aggacaacaa aaaggctcaa tcatttatca agatgttatc tctcacgaca attcgtatct
     1621 tgaaaaaatg ggcttgtacg atcccaaaac agatacctta gatgaagaag gtttgaaagc
     1681 agctggaaga aaaatgatgg agacattatt cgagaaagaa caattgaatg aaacaggtta
     1741 ctggatggca acgattcacc gtaacactaa gcatatccat attcattttg cagttgttga
     1801 aaaagaaaat acgcgtaaac cgtatactga gattgaaaat ggtgtgagtt atatcgtacc
     1861 gaaggggaag agaagacaag caacattaga cgcaatgaaa accaattatg tacatgagtt
     1921 agaacgtttc tcttatgaaa aggatcgaat gatcggtatg gagaaacgaa atttattgac
     1981 acgaaaaagt gatttgcgta acaccttaac aaaagtagta aaagaaccag cacgttacga
     2041 tcgacacgcc atgaacttat taaatgaagt atataaaacc ctacctgaaa aacgttcgga
     2101 atggaattat ggagatgaaa atcgaaccaa gctttcacca tttacaaggg gaaaacttga
     2161 tgagttaacc aaatatgttt taacaaaaga acctggatat caagaatata tacagatttc
     2221 aaaagcattg aaagaagaag ccaaaagcct ttatggggaa agcaaaagag aaacaaagaa
     2281 tgcagaaaaa aacgccctct ttgatttaaa aaagcgcaca ggaaatgcga ttttaacaga
     2341 actgaagaga caggatagtc aaataaaacc attaatagaa agtgtcaatc agatgcaatc
     2401 tatttacaat gaacgatcct ttcgaacact caaacagttg ccaacaaatg aatacaagta
     2461 tagttatcaa gaattcgtaa agcgtaggaa acaattccaa caaagagttg tcacgcagcg
     2521 aagcttgcgt caattaaatc gaacaattaa tgcacatgaa gaaaaatacc acgcaagtaa
     2581 agcatatgaa gagcaacagc gaagaatcca aatggaacaa gaaagacaat aggaggaaga
     2641 gaaatgaaac aatatttaga tcaatggaaa gtcatagaag gatcactaaa agaagaacga
     2701 atcgaacaac ttcctgattg tttagaaaaa gagcatttgt ttcagataag cgaaatgttg
     2761 agaaacgaac agtttgatcc aaatcaattt ttagttgttg agtacccagc tactggtgtg
     2821 tattgttgta atcatgtgaa gggtgaaaaa tattttatta ttcaagaata cgaaggtaag
     2881 ttagcgcctt attatactac ttgggaaata aatgaagagg gaatcaataa tttcccatgt
     2941 gaaagcattg aagagtctat ttctctcacg gaatgttaaa taaatagaga cgtaaaagag
     3001 tcctgaacgt gattgtccag gactcttttt caatgaattg ttttccgcca attcactgag
     3061 tggtgcgatt caattttatt atatcaaaag aaagtgagat tccaaagtga agggaggaag
     3121 aagaaatgac tcagacaacg attggtgttg tggttgaaaa aagcagggat aatctcgttt
     3181 ttgtgacaga aattcaaaca ggtagagctt ttgtagtcac agacaaagct gcaaaagcgt
     3241 atcaaaatgg caatatttta acgttaaatc tgactacgaa gatgttcgtt gatgcagtag
     3301 aaaattatcc atttgtttag acaaacgata ttttacaagg aggaaacgga catggcagaa
     3361 gaaaaagaac cactagataa tagtcggttg ggaaagtcca aaagaaaatt agtacggttg
     3421 caaaacgaat taaataatca atttcaaaag ctatttgatc accaaaaatt gacaaatggg
     3481 caaccaatga atgacaaacg aaatggtcgt tcatggttta accagcaaga aaaaatagaa
     3541 aataaaatac aaagtttgag agaagagatc aagcaacaag aaaaacaggt agaaaaactt
     3601 gaaagacaag aagagctaaa ggaaatgggc tataacaagt acggcggact ggatatgacg
     3661 atcgataata ttccaagaat caaagaagaa atcgaacgct ttgaaaaagg tgaatcgact
     3721 ttttcagccg ccacgatccg aaaatatcaa cggaagttgg aaacattaga acagctgaaa
     3781 gaaaaatcag aaaaaggaaa agaaaatatc ctccctgaag tacaagcgat cattgacagc
     3841 ggacgagtga cgcaatggaa aaaaaatcca accatttatt ttttgaaagg ctatcgaaaa
     3901 gttgctttag aactgaatga aaacggggga tttgaggaat caaaaaaata ccaagccacg
     3961 tcggaagagg aaaaagcagc tgttcgtgaa ttacttgaag aggctgcacc aactgattct
     4021 ctgaaggaga aagaaaaaga tcatttctat cgagtagaat tcaacgaaaa tgatccagaa
     4081 aaaggattga aaagttatgg tggggagatc gtaacattag aattgatcga tcaacttaaa
     4141 acgttggata accaatacgc tgcagaagca ggttattata aattctattt tgaagaagtg
     4201 aagaacggag aagtagtaga tcagatccga atggatctag gggatggcgt caacagtaat
     4261 ca
//
LOCUS       DAKBVG010000192         4214 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000192 DAKBVG010000000
VERSION     DAKBVG010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4214
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.192"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(117..1883)
                     /locus_tag="OUD09_002320"
     CDS             complement(117..1883)
                     /locus_tag="OUD09_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT9693555.1"
                     /translation="MQMIGGLTMKSFYKNKFALTNQGAEDLVKASISSFFVYCINMVP
                     AFIIMMLIDELVLENVKPPWLYFTVSFVTLLFMYWLLDREYENLYNSTYKESAHLRMQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKIVDQGTHSELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1843..3597)
                     /locus_tag="OUD09_002321"
     CDS             complement(1843..3597)
                     /locus_tag="OUD09_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT9693556.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLK
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(3707..>4214)
                     /locus_tag="OUD09_002322"
     CDS             complement(3707..>4214)
                     /locus_tag="OUD09_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HCT9693557.1"
                     /translation="FSIMVSSIGGAFGHSISPGIFGLLSGIIFVYISYNYPRKGIFTI
                     YTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVFGYDRAIPQVASWALMQLGSAA
                     GQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGIWGIISVLVVASLSMIGVLIGR
                     KVLKKYKK"
BASE COUNT         1417 a          800 c          588 g         1409 t
ORIGIN      
        1 atcatactga ttctttcgtg tattcaaaat tagatgctgt ttaataaaat tattcttttt
       61 gaaaaagaat tataactata tcccaaaaat tgtaggttaa tttaaagcct atttcatcat
      121 cgtgattgag cttctttttc atagacgaat ttctcagata gtttagtttt ctttatgaga
      181 tttttgtata tttctgacct tcgatacaac tcactatgag taccttgatc aactattttt
      241 ccttgatcca tcactacaat tttatttacc ttttcaatag acttcaagcg atgcgatata
      301 attagtactg tcttatcttg tattaaacga tttaaacttt cctgaatacg tttttcattt
      361 tccgcatcta aactggcagt aatttcatct aaaattaaaa ttggcgcatt tttcaaaaaa
      421 gcccgtgcta tggataggcg ttgtcgctct cctccagaaa gtgaacttcc gttttctccg
      481 accaatgtat gataaccgtc aggtagtttt tcaataaagt cttcgcagtt agctaaacga
      541 gctgcttttt tcacttcctc atctgacgct tgactatttc caatacgtat attttccaaa
      601 atacttgtat taaacaaagt tacctcttga aaaacaatag cgattttgct aaacaaagaa
      661 gctggagata cacgtttaat atcataccca tcaattaaaa tacagccttc atcataatca
      721 tacagtctag acactaattt caagatacta gttttcccgg aaccactagc acctactaac
      781 gcagtcactt ctccttgttt agccgtaaaa gagatatgat ctaaaattgg ggtattgtta
      841 tcatatgaga aagagacatc tcgcaactca atgtcaaaag atttcagagg actatctgat
      901 ccctcttgga tactggtttc cttcatagct ctaattcgct gtattttagg agctaaataa
      961 aatatttcaa gcactgcttc tttcatagaa tcgaaggaat cctttatttt tattgcggct
     1021 agtaagtaac caacgacgta gagtatagtc acttcacccg ttaacagtaa gtgaacacct
     1081 actagaatga ctactgctaa actaacatat gagaaaatag aggataaggc catgactgaa
     1141 aaagtactta attctacctt taaatgaatc ctttcactct cttccatttt tttgaaaagt
     1201 ctgtcttgaa cttttttaga tagattaaag ctattaatct cttgctgcaa ttctatagtt
     1261 tcttgaaatt cttccgagtt ttctctcaaa gtatcgtaat atttagtatt ggctttcgtt
     1321 tgggatttct ttgataacaa gattaagaca aaactaaata acgttggcaa aataacagct
     1381 aatcccattt tgacattacc aaccaaaagc atcactgaaa taaaagggaa aaacagagcc
     1441 ataccgccgg ctttaggtat tgcatgactc atcgcatgct caataccttc aacgtcagac
     1501 atgatagttt gagataaatc tgataaatta tgttttgaaa aataggataa tggtaaattt
     1561 gacaaatcgt ctgcaatttg cattcttaaa tgcgcacttt ctttataagt actattatat
     1621 aagttttcat actccctatc taacaaccaa tacatgaaaa gcaaagtaac gaacgatact
     1681 gtaaaataaa gccaaggcgg ctttacattt tctaaaacta attcgtctat cagcatcatt
     1741 ataatgaatg ctggtaccat attaatgcag tatacaaaaa agctagaaat agatgcttta
     1801 accaaatctt ctgcgccctg attagttaaa gcgaatttgt ttttataaaa acttttcatt
     1861 gtcaagcctc caatcatttg cattctcata attagtgacc aattgtttat acacttgatg
     1921 tttagccatt aattcattat gatttccacg ttctactatt ttcccttctg acaagacaag
     1981 aatttcatct acagatttaa tgctggataa tcgatgagct atcataataa ctgttttttc
     2041 tttcatcaga tgttgaaaag ccttttgtag ttcatactca ttatctggat caatagatgc
     2101 actggcttca tccataataa caatctttgc atcttttaaa attgctctgg caatagctat
     2161 acgttgtttt tctcctcctg acagataaac tcctttacta ccaattaagg tatgctctct
     2221 ttcgggaaat ttttctaaaa tcgattcgca accggctaat cttagcgcgt caaatacttt
     2281 acttgctggc gcttgaggat ttgctataga aacgttatcg taaatactct gattgaatag
     2341 tttcgtatct tgaaagacaa aagcaattgt gttaataatt gtgtcttttg aatagtgatt
     2401 taaaggctta ccaccaattt taatctgtcc cttatccaca ttgtagaaac ctgaaattag
     2461 tttagctatc gtactctttc cgcttcctga ttttcctact aatgcataaa ttcttttttg
     2521 ttctaaaact aagttgaaat tttctaaaac acactcttct ttgtatgaaa agctgacatt
     2581 ttcaaatgca atgtcacaat ttttaatctg tgtctcgttc ccatatttta gttggtcttg
     2641 cttcatttct aaataaaggg agtctagtgt ttcgactgca tatcttgctt caaacaagtg
     2701 catacttaca tacattattc tcatgaaaga aacaaaaata attccactta aaaaaaacaa
     2761 catcaacaag tcaacagcta atatcttggg agtggtaaga gaagttataa aaaaggttat
     2821 aggaactatc aaaatgcaaa ctatgccaaa aaatagccat tgaaaaagaa caaaaggtgt
     2881 tttcccactc aaagaatact tatatgcaaa cttagcataa tcgttaattg ctttattcaa
     2941 ggctttaaat gatgttactt tagcaccaaa tatctttata acttgaattc ctcggatata
     3001 ttctaccgtt tcagagctta acacatctaa cgattcttga tatttttgaa taaaatttgt
     3061 attctctgtc attttcttta acaagaatac accaattact aacatggcga ttagaaccaa
     3121 tccaatttta aaattaatga aaaagcctaa ggcaactgat aaaacaggtg ttactatggc
     3181 ttgagcacta tctggaatca agtgagcaat tgccatatgt gttttagccg cgttgtcatc
     3241 aattgtcttt cgaatatatc ccgaagaatt taaatcaaaa aaacgaaaac ttgcttctgt
     3301 taaaccgcta attccctttt ttctcaaatt agtctctaag cgaaacccaa ggatatgaga
     3361 aaataagcca gaaaaaatat aaaataaagc tcctaaagtt aaccaagcaa cagtttgaac
     3421 cgcataactc ttagctaaat attcatctcc tacaataatt agagacttaa gaaatttgta
     3481 aatactgtaa tatccataga ctgttaatac gacagaaaaa tcagaaaata aagtggctaa
     3541 ccatccgtag tatttcattt tagggacata actaaataat tttttgtata ctttcatttt
     3601 ttcctccccc gcattcaatt tcttctctta ttcgtttaca actgttaatg ctttccgtaa
     3661 tagcgtaaat tagagatgta caaacagata gaaggtaaac tacgttctat tttttgtatt
     3721 tttttaagac ttttcttcca atcaatacac caatcataga taaacttgct acaactaaca
     3781 ctgaaatgat tccccatata cctaccgcat agtggatcat tgcatccatt gttgcagcag
     3841 attgtccttt atctatccaa tcttgacgaa aacgatctgt aaagaaccat ataggaatcc
     3901 actgtccggc agcactgcct aattgcatta gagcccaaga cgcaacttga ggaattgctc
     3961 gatcatatcc aaatacagat agaataatat cagctaagat cgccatacta atcgaactga
     4021 taaaccaagg taagtacgcc ccgcccatta acgtaaaaaa taacaataaa acaattgtgt
     4081 aaatagtaaa aattcctttt ctagggtagt tatagctaat ataaacaaat atgattccag
     4141 ataataatcc aaaaatccct ggggatatgc tgtgaccaaa cgccccacca atactactaa
     4201 ccattattga aaaa
//
LOCUS       DAKBVG010000193         4212 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000193 DAKBVG010000000
VERSION     DAKBVG010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.193"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(50..592)
                     /locus_tag="OUD09_002323"
     CDS             complement(50..592)
                     /locus_tag="OUD09_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HCT9693558.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            726..1115
                     /locus_tag="OUD09_002324"
     CDS             726..1115
                     /locus_tag="OUD09_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693559.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1119..1883
                     /locus_tag="OUD09_002325"
     CDS             1119..1883
                     /locus_tag="OUD09_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HCT9693560.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRGSEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            2012..2959
                     /locus_tag="OUD09_002326"
     CDS             2012..2959
                     /locus_tag="OUD09_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCT9693561.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3089..3829
                     /locus_tag="OUD09_002327"
     CDS             3089..3829
                     /locus_tag="OUD09_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693562.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKGNLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKMLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(4115..>4212)
                     /locus_tag="OUD09_002328"
     CDS             complement(4115..>4212)
                     /locus_tag="OUD09_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCT9693563.1"
                     /translation="YLEIGSVLPQLEAKQTTETFEPLDPLEQLEH"
BASE COUNT         1395 a          704 c          850 g         1263 t
ORIGIN      
        1 taacatttaa aatttaattt agatattaga cagtttttaa gtttgtgagt tattttcctt
       61 gtcgttttct cactttttta gtccagaaac cacctgaaat atattttggc tcataagaaa
      121 tgataaaagc acgctcttcg atttctttga tcaggcgata cagttcacgt tcgctttttc
      181 taggagaaag tatttcgaga actagccgct caccttccag ccccattgca gaagtctgtg
      241 tcacaccata accgtgttct ctcagtatac ccggcaaact tttatcttct gcgctatttg
      301 ctggcaaaat cgttgtgacc atgatgtatc ctaatgccaa tttgtcttca atcaaaattc
      361 cgacactgat acctactgcg taacctaacg catagacaaa taaattcagt ggattatcta
      421 aacgatctaa aacaagggat aagccgacga tataaatcgt gatctcgacc atactgacaa
      481 gtggagcaat cagtcgatag cctttcattg tcaacatgaa acgtaacgtg ttcagagtga
      541 tataagcaaa attaatgagg aagatcatcc ccaacatttt tggatcaatc atttgtaaaa
      601 ctcctttcat ttatataaca acaatatcaa aaaaaatcgt aagtgaagaa aaattgaaac
      661 tttatacata caaaaaatga taaaatgatt ataatagaat tataaagata ggttaggtga
      721 ttaaaatgaa aacggtcaat cgtgtgtttt gtgccagcca cgtgctaagt ctccttctca
      781 gtatatactt aggtgtctat tcgattcaac agccaattga ggtagagaac attgtttttc
      841 agatgtcgct gacagatggt ttaattttaa gcgttttttt ctttttgata ctgtttattg
      901 gtaatatttt cggtagcgtt gccagttttt tgaatttttc tatttatcca ctcctttcgc
      961 tagcattagg agtggtggga ctttgttctc tgtgcttatt tcccgagcca ttcagtcctg
     1021 catttatatt atttggtaca ctgaacctgt tccagacaac tgttggagca tggttgctat
     1081 ggcgatcagg aaatttaatg aaaataggtg agtaacaatt gaatccaatt atattgaaac
     1141 gttctgtcca tcatattcca gtgttagaag ttgttctgaa agaattaaga gaggaacgta
     1201 ttcccgttgt catctattat cacggatggc agacaagtaa ggagttagtt ttgacgcaag
     1261 gaagaaaaat tgcgcagcag ggcattcgtg tgatcctgcc agatgcgatg aatcatgggg
     1321 agcgcaaaca gccggtttct tctattccat cctatacatt ttgggatagt atctacggaa
     1381 atctgtttga atttgacaca cttatcaagc atttagaaaa aagaggtctt ctacatgatc
     1441 agcttgcagt tggcggggtt tcgatggggg gaatgacaac atgcgccttg ttggctaaac
     1501 atcctcatat agtagcgggc gtgtgtttga tgggttcccc tgcacctttg gaatatggaa
     1561 aagaaatcgt aaggcgcgga tcggaatata attatgtcgt accaaaagac tattttgatt
     1621 tgatttcatg ggttaagagc tatgatttgt cattgcagcc ggaaaagtta gctggacgtc
     1681 ctctgttctt ttggcatgga caagctgacg aaaaaatccc ttatatacaa acggaacgat
     1741 ttgtagaaaa aaatcggcat aagtcttacg gaaaagaaat tgtttttcgt tcctcaaaaa
     1801 cacagcgaca tatggtacaa ataccactaa tggaagaagc ttccactttt ttagcaaaaa
     1861 atttgataaa aaagtataaa taattgaaac ggaaaatcac tcatgacgat agaggatttt
     1921 ccgtttttta ttttgggcta taataaactc tgttaggaaa atttggtata attgatagta
     1981 agaatgacaa catgttagaa aggaagagag catgcccact ttagcagatg ttgcaaaacg
     2041 tgccaatgtt tcaaaaatga cggtctctcg agtcatcaat catccggaac aggtgactga
     2101 cgaattgaaa gagttagttt tctcagcaat ggctgaactg gattaccgtc caaatattgc
     2161 tgcaaaagca cttgtcacga atcggtcgca gatcatcaaa ttatttatac tggaagaaat
     2221 cgacacaact gagccctatt acatgaattt gctgatggga atcgctaaac ggatcggtaa
     2281 agcgcattat tctctccaat tagttacaaa tgatgccttc gacgtcggtt cttgtgatgg
     2341 atacatcatc acaggtgtca gaaaaaaaga cttcgattgg atcgatcgtt tggaaaaacc
     2401 agttgtcttg tttggggaaa attctgaagg atatgattat gtagacagcg ataatgaata
     2461 tggtacagca aaagcaacgc aatatgcctt agaaagagat tatcaaacga ttatttatat
     2521 cggtatcgaa gtggacgagc cttttgaaat ttcaagggaa actggttatt tatctgtgat
     2581 gaaggaagtt gatcttccta gtaaaatctt gcggtttcca aatcattcga cagaagtaga
     2641 aagatatatg acagaaaact gggcagattt tacagataaa acttgttttg tatgtagttc
     2701 agatcggcta gcactaggag cagtaagagg gattataaat gctggaggac acttgcccga
     2761 agaatttggt gtgattggat ttgatggggt atttttagac caaatcagtt cacctaaatt
     2821 gacaacgatc aagcaagata ttatacgtat gggtgaagta tgtggagaaa tgttgttaaa
     2881 aaaaatagaa gaaaaagggg cgagtcaagg ttatcgtcgt ttttttcctg aactagttat
     2941 cagagagaca acgaattaag tcttattact atatatatat ttatgaaatt catcgctatt
     3001 ttcttaaaat aatcagcgca ttgctactga aaacaatgaa tctgtgctat tattgagaaa
     3061 agttccattt ttgagaagga tggtttgaat gaaagaaatc agacgaaaac atttccgttt
     3121 tcctgcgtac gatgatgagt taggcgtaaa attaaccaac gacaagaagc gagtattatt
     3181 tgacggacaa gaagatcttt tgactgaaca gaatcagtcc ccgtttgagc agatgaaaga
     3241 atttcatttc acgaacacga acaccgagaa aatccgtcgc aagcgtcgtg caacaaatgg
     3301 attgaagtca gctccgcgta agggaaattt ggaaaagcat aaaggaaaat taccggatta
     3361 taccggccat taccaatcag aaatcacatc gacaggacga aaaaaacttt ttggtgattc
     3421 ctctcgttct gtcagacaaa gtgaaacaag gacagagtcc aagcatacta cacgaaacgc
     3481 atctgttgac acgaaggtgc gggagcgttc ttattttgtt cctaaatatg tacccgcatc
     3541 gattatccca gaccctagag aaagcaaaat ttctgaggat catttaatcg aatcgatgaa
     3601 aaaaacggat gactcttatc tgttgttcga tactgaaccg acgccgtatc aagaaaaaag
     3661 aaatggtgag ccgtctgtac gaacattcaa tcgaacaaaa atgttagagg aaacaaagca
     3721 gtcagaagaa aaaaaatcga aaaaaagcca tggtatttta gaacgctcat tacaaggatt
     3781 gatcgatgat caaggaaact ctttgggaga aaatagctat tttaaataag tgatatacaa
     3841 atttatattc ggctacagtt ggaaaggaaa cgctgcggcg tttccttatt ttatactcta
     3901 tccggaagtt tatatcgtat cctcagattt ttttgagctt aagcgaattt tttctcccaa
     3961 taagtaagaa aataatggca aaataaaaaa acaaactatt ttcgcagatc gtttgcaaaa
     4021 atagtttatt tttttataaa gaagatttta ctgaataata tcattttagt ttacataatg
     4081 atatttctgt aaataaagta gtgaaaaata agaatcagtg ctctaactgt tcaagagggt
     4141 caagtggttc aaatgtttct gtagtctgtt ttgcttccaa ttggggcaaa acagatccaa
     4201 tttccagata tt
//
LOCUS       DAKBVG010000194         4209 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000194 DAKBVG010000000
VERSION     DAKBVG010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.194"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(<1..958)
                     /locus_tag="OUD09_002329"
     CDS             complement(<1..958)
                     /locus_tag="OUD09_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCT9693564.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVR"
     gene            complement(1203..2474)
                     /locus_tag="OUD09_002330"
     CDS             complement(1203..2474)
                     /locus_tag="OUD09_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCT9693565.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            complement(2758..3705)
                     /locus_tag="OUD09_002331"
                     /pseudo
     CDS             complement(2758..3705)
                     /locus_tag="OUD09_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            3717..4190
                     /locus_tag="OUD09_002332"
     CDS             3717..4190
                     /locus_tag="OUD09_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCT9693566.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
BASE COUNT         1337 a          801 c          660 g         1411 t
ORIGIN      
        1 ttctaacttc ttttattcgt tcattaacca tgcgaactgc tagttttcca atttcttctg
       61 tattcacatt caccgtattt aatgcaggtg ttaaaaattc agcaacttca atattatcaa
      121 aactcacaat cgaaatatct tctggaatat gtagaccatt tttttgaagt gaacgataaa
      181 cccccacagc aataggatca tttcctacca tgatagcagt cggcaactta tctttagcat
      241 gttttttcaa aagtaaatct gctgcctgca ttccgtcaag tgtggtccag ccttctaaat
      301 aaatttgact atatttttct aatccttttt gtttcatcca cgtctcatag gctatttttc
      361 gataatcatc atctgacacg tgtttatttc cttggctatc taaacgaaca cgtcctcctc
      421 cgataaacgc aatgtttcgg tgtccttttt tatacaatcg atctaaatgc tgattcgttg
      481 cttttgttaa atcagtataa actgcatcta cttcagcttc aatactaggg tcatcaatca
      541 ctacgacatt cgaattaatt gctgatactt cttcaatcac agaagatagc acctgcccaa
      601 taatcaacac accttgacat tttagcaaat cgtcttgctc caacggtgtt tctgacaaac
      661 gaataatttt cttcaaattg actttgcttt gttctgcttc gacttgaatc cctctgcgaa
      721 tcgttcgaaa atagggatct tccaactcat ctacttcaga aactgtcgtt attaatgcaa
      781 tatttgttaa attttttgca acgggacttt ttttgacata atgataaaaa ttaaccgatt
      841 ctgcaatctt tcgtttggtt tcatcggtta ctgaaagtgt cggatcctca tttagcacgc
      901 gcgatactgt tgcaatggac acacccgccc gttttgcaac atccctaatt cctaccataa
      961 tagctcctct tttcatattt gaaagtgttt actaacatat aatcatacat tttgaaaata
     1021 agcaaatttc gttaaaaata ggcactgtaa tcataattaa aaacaagaaa aaatatccga
     1081 acaatttaga gtaaataatg ctaattctag tgaatttgcg acgaggaact gctagatcac
     1141 agttcattct ctctatcgtt cggatatttt attgttaact atacttattt cacagagctt
     1201 ttttatttga ttttgtgaac agagtctcga gaaatcaatt ctccctctaa tagtagtact
     1261 tgatttttta tttcttcttg tttggccagt ccaatcagtt ggtgaaaagc aatcgccccc
     1321 ttgttacctg gatttagtcg tattgttgtc aacgcaggat ccattaattc tgcaaaagta
     1381 atatcatcaa atccaataat cgataattgt tccatcactc gtttattctt attttttaaa
     1441 aattttacta atttagccgc taaataatct ccagtaacaa caattgcagt aaattcagct
     1501 aattcttgat aaattcgctc aaattcagag ttgttatata tccaatgcga tttaaaggtt
     1561 aaatgatatt tctttaatgt atcgatataa gcttgatgtc gttcttgaat aacacttggt
     1621 tcacctacaa tgtagtcaaa agaaagaaac gctattttct catgaccata atgaatcaaa
     1681 tgttccactg ccaatgtact agcttggtaa tcatttgtat ttacaaataa acgattcatt
     1741 tgttgttcaa gttctcttaa tgtctctttt gaaagatgcg tatcaatata aacaacagga
     1801 ccatcaaaaa cgttatttaa ttccaaaaat tcttttacac gtacaccaac aacaatgaac
     1861 ccctcaaaac gccaatttct ctgaatagtt ttaacatctt ctgctgaatt aacattatgc
     1921 accaatacaa aataaccatg attctttgcc tgttcctcaa ttccagacaa aacttctgcg
     1981 gtaaatggat cagaaaatac aaaatcagca ttatcaatag ccgaaaaata aagcaatcct
     2041 attaaaaatg caatcgtctt accgattccc tgacagacag aacacattcc actaggatca
     2101 ttaaatgaat aagcatttgc atatcctaaa ctaggtgttg caaaacatga aaacaacaca
     2161 cgaaataacg cattgatgtt agtaatcgtc cctaacgttg agcgcgcact gccaccaatc
     2221 ttcttttgat tgaccacaat cgccgtagaa agattatcaa tctcatcaac ctctggacga
     2281 ctgtatttag gtaaaaataa acgggtaaaa ctatcatagg tgtcattcag ttgacgacca
     2341 gcttcttggg taatagtatc aaaaacaatc gatgattttc ctgaaccaga tacgctggta
     2401 aaaatactaa ttttattttt gggaattttg agactgacat ttttaagttg tttctcttgc
     2461 gtggataatt tcaatatact cgttcatttg gtactcccct ttattttgaa atagttgaga
     2521 atgtttcaat gagacacggt atttttggca gatgtttagt ttacctagaa agaggtataa
     2581 aaatcaagaa aaaaagagag gctgactcat aagtagtttt attagtcgaa aatgatagtt
     2641 tataaaaata ggaaccgcat caaatcaaca ttctttagaa tggatttgat gcgtttttct
     2701 ttattttttg acttatgagt caacctctta ctcttcgtca tggatttgat tagctaatta
     2761 ttgctccact tgttcacgaa tgaaatttaa gtactgcggt aaaaataaag gaatttcttc
     2821 aaagcgtgga ctttgatttt catacgcttt tgcatctagg tttccttccc ctgtagcaat
     2881 ttcaatcaat aattgatttg ggtcacgata ataaagtgat acaaaaaatt cacgatcttt
     2941 tattcctgaa tttataaaat tgcgctcttg taattgtttg tcgatgcgtt gtaaatcaga
     3001 tttattttct acgccaaacg caacatgatg gacgctacca atcccttcaa tggcaactgg
     3061 attctcacga tcttgaataa tctgaacttc ttgatagaac tgagtgtctt gattttctaa
     3121 gattgtcact cgttgatgac ccgaaaagaa actgacgtat tctttgtgct accagcctac
     3181 cgacaataac tctcgttcag tcgcatcgga atattgtacg cgtagttgaa tggcatcaat
     3241 tcctaaaatg gcatgctctg tagggatctg gtctgtgaca tacgggaaat aatcatcaat
     3301 atcttcaaat tcgcgtaagg gaactaaagc taatcgtgtt gcatctggcc cttcaaaacg
     3361 taagataggt cgtccagaaa atgattcgat tccataatgg caaacgcctt gtgtttcaaa
     3421 atatatttcc caaaaatgca atgatgcttc ggaaggaact tttagtatcg ttcgttcaat
     3481 cgtattggtt ccaaaaaact ggttgttccc ttcttgtact tcaaaaaaag tcagttcagt
     3541 tcccacacgg tggtgattat ctgaaaaaaa caggtgatac atggtatgat cgtcttgatt
     3601 aattgttttc attagtaatt ttaaaccgag tatattatga taaaagtcaa atgtcggtcg
     3661 aataaagcga ttcacaatag aaatgtgatg aatacgtgcg ggcataatat ctaaaaatga
     3721 gaatcaaacc tataaaagat ggtgaacaac ttttgttttc atcagaagaa aataagcccc
     3781 tttatttaga ctatgtaaat cacaatttga aaatcatcat taaagagaat aacttaaaac
     3841 atatcacact tcatggtttt cgtcataccc attgtagtct gctctttgaa tctggtgcgt
     3901 ctgtcaaaga agtgtatgtt cgacttggac atacagatat taaaaccact atggatgata
     3961 tatacaccca tatcgcgaaa aagcagacag aggaaacggc aattcgtttt gcagatttca
     4021 tgagttctac agaggaaaag caaggaagtt tgcagaaggt caaaagtatg gtcaaaaacg
     4081 aaaaaagcag actatcccta ttagatagtc tgctaaacgt tgagttatca gtatttttct
     4141 tattccgcag cgtttgcgtt tttgagaccg tattgattct ctttttttaa tttcatcctt
     4201 ttccaacct
//
LOCUS       DAKBVG010000195         4208 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000195 DAKBVG010000000
VERSION     DAKBVG010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4208
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.195"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(42..1271)
                     /gene="rpsA"
                     /locus_tag="OUD09_002333"
     CDS             complement(42..1271)
                     /gene="rpsA"
                     /locus_tag="OUD09_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCT9693567.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(1406..2086)
                     /locus_tag="OUD09_002334"
     CDS             complement(1406..2086)
                     /locus_tag="OUD09_002334"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCT9693568.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAIQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(2135..2770)
                     /locus_tag="OUD09_002335"
     CDS             complement(2135..2770)
                     /locus_tag="OUD09_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCT9693569.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(2836..4176)
                     /locus_tag="OUD09_002336"
     CDS             complement(2836..4176)
                     /locus_tag="OUD09_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCT9693570.1"
                     /translation="MIQALLDGEDTLAILPTGTGKSLCYQLTGFLMEGLVIIVSPLLS
                     LMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLSQYKFLFLSPEMLTNPSLLEHL
                     KKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLLGNPVTLALTATATDLVARDIR
                     HVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHFMERAQGAAIIYCATKKEVERL
                     YHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFLIATNAFGMGIDKSDIRYVIHY
                     DLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIHYFFNQLSREQRQSFELYLEYA
                     TEQAPFDELQKKWMELIQQSEKPENWVERLKHQEKEKEFRLQQMLRYINEENCRRKFI
                     LAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQLHWETILLNLFKKNMEH"
BASE COUNT         1099 a          918 c          737 g         1454 t
ORIGIN      
        1 aaaagcagct gctttcatct cagtcacaga tacaaaatca attatttttc agaatcatcg
       61 gttgcttttt cttctacttc ttctttcaac gcatcaccta aaatgtctcc catagtaaat
      121 ccagtatttt cttctggtaa ttcataagac tcaacatttt ttggttcttc ttctgaagct
      181 ggtttttctt ctaaagcttt gatactcaat gcaatacgat gttcttctgg atggatctct
      241 aatactttca ctttcacttc gtcaccttca tgaagcactt catgtggtgt cgcaatatgt
      301 ttatgtgaaa tttgagagat atggactagc ccttctactc cagggaacac ttcaacaaag
      361 gcaccgaagc ttgtcaaacg tttaactgtt ccgtctaata ctgtgccaac tggagctttt
      421 gtatcgattt ctgaccaagg tccaggctgt gtgtctttga tggacaagga aacacgttct
      481 tgttcaggat tgatagacaa cactttgaca tttacatcat cattgacagc caatacatca
      541 cttggttttg ctacatgact atgtgaaatc tctgagacat ggactagacc gtcaattccg
      601 cctaggtcga caaatgcgcc aaaatcagta agacgtgcaa cacgtccact gagaacatct
      661 ccctcatgga tagttgaaag aatttctttt ttcttttctt ctctttcgtt ttccactacc
      721 gctttatgcg ataagatcaa acgattttct gatggttcga tttcgatgat tttaaagctc
      781 aatgttttgc ctttatagtc tgaaaagtct gcaacaaaat gatcttccac cattgatgca
      841 gggacaaaac cacgtacacc gacgtcaacg actaaaccgc ctttgacaac atttgtaaca
      901 ggtgcttcga tcaccttgcc ctcttggaaa tctctttcga tatcttccca aactttttta
      961 gcatctaaac ggcgttttga aagtaggtag ctgccgtttt ctttatcttt gccgatcgta
     1021 ctgataacga ctaagtctaa tacatcaccg acttttacta cttcgctgat atcttctacc
     1081 ggcaaagttg ataattcttt tgccggaacg actccttcaa ctccagcccc ttcgattccg
     1141 acaatcactt gtttgtcttc aatgactaaa acttcgccct taacaacatc gccaactttt
     1201 acttcttgta cgcttttcat tgcgtcttcc attgactggt tgttctcttg attttgatca
     1261 aattctgtca taacacttgt cctcctaacc aacattctgc cgtaagtacg gtactgtttt
     1321 tagtttaatt gattattcaa aaaaaataaa gcgatttcgt tcgttttcct tgaaaaaaca
     1381 attgtttttc attttttttc ttaatttaaa aaagaagata accttttttc aaaacaacag
     1441 cttttattgc tgcaacgact tctggaatcg atttgccagt cgtatcgata cgaacagcat
     1501 cctctgcttg cttcagcgga gagacgtccc ttgttgaatc ataatggtct ctttcctgta
     1561 tctctttcgt cagtgtttca agatcagtcg gaattccttt ttcttggttc tctttgaaac
     1621 gtctttctgc acgctcactt acgcttgcta ctaaaaagat tttcacttct gcattcggta
     1681 atacagctgt cccgatgtct cttccgtcca tgacaacgcc accgcgttcc cctatttttc
     1741 gttggatagc aactaattct tctctgactt ttccatgtgc agagacttca gaaacagctt
     1801 ttgtgacatc tggttgacgg atttctaaag tcacatcttt tgatgctatg aatacatgct
     1861 gtccatcttc ttgttgcgaa aaagagatag gatacttttt gatcagcgaa actagccctt
     1921 cttcatctga aaaggataca ttgttcgtaa tagctaaata agttactgcg cgatacatgg
     1981 caccggtatc tgtataaata taattcaatt cttttgctaa tatcttggca acggtacttt
     2041 tcccagatga agcaggcccg tcgattgcaa tactgatttg tgtcatgata taactccttt
     2101 ttctcaaaaa aaaagccgca aagcgcggct attattattt aattcttaat tcttgaccag
     2161 ggtaaagcat aaagttgttt ggatctaagc cattcaattc aaacaattga tcagtggtga
     2221 tccctgcacg ctcagccact tgttttggac cttcgcctgc ttgaacagtc gttgttgaac
     2281 cggctgcagc tgatgaagaa ggtgttgttt ctgctgcatt gctgcttgaa tcccctgctt
     2341 gataagttgc ctcacttgag gaagtcggtg ttgtctctgc tgcaatactg ctttctgacg
     2401 ttacacctgt atcttgagac acagtagatt cttccgcgat tgtactagat tctactgttg
     2461 aagtctcagt tgactcctct tttgttgtac ttgtagaact ttctactact gatgtcgaag
     2521 tttcagaaga agttgctgct gttgcattgt tgcttgatcc atttctgatc cagaaataag
     2581 cacctgctgg aatagcgata cataagacca gtaaaatcaa aaatagcgaa agaaataatg
     2641 tatttccttt cttttgttgt ctctgagaac ttcttgatga aacttcatca tctgtatcgt
     2701 agattggttg ttcccatggt tcttgtgttt cgttattttc tgtagattga tgtctatctt
     2761 ttctgctcac tgttttacct cctaaactct atcgtgtgta ttataccata ggaaaaagtg
     2821 tcttgtccta aaaaatcaat gttccatgtt ttttttaaat aaatttagta aaatcgtttc
     2881 ccaatgaagt tgttcagtct tagaaaaaag cttcctcttt ggcagaagtt ctgcttttgc
     2941 tccatcaata tcacaacaat tctgcggctt ttcggagagt ttttcgccaa aataagctaa
     3001 gatgaatttt ctgcggcagt tttcttcatt aatatagcga agcatctgct gcaggcggaa
     3061 ttctttttct ttttcctgat gtttgagccg ttccacccaa ttttctggtt tttcagactg
     3121 ctggatcagc tccatccatt ttttttgcag ctcatcaaag ggagcttgct ctgtggcata
     3181 ttccaaataa agctcaaaac tttgtcgctg ttctctagaa agttgattaa agaaataatg
     3241 gatacgttca tccccacttt gataaaaaag aattgctgca ctttcttcct gatctctgcc
     3301 tgctcgaccg atttcttgta catagttttc tagactgtct ggcaaatcat aatggatcac
     3361 ataacggata tcagatttat cgatccccat accaaatgca ttcgtagcaa tgagaaactg
     3421 cagctgattt tttacaaact gctgttgaag ttgtcttctt tgagctgcat caagaccacc
     3481 gtgatagtat ccaactgtaa aacgttccct gaataaatga tacaatcgtt cgacttcttt
     3541 ttttgttgca caataaatga ttgccgctcc ttgtgctctt tccatgaagt gttcaagttc
     3601 ttgttctttt tgctgcgttt tccgaacaaa aagcgagata ttttgccgat tgactgattg
     3661 tctgatctct tttggtggtc gatcaaatag tacatgacgt atatctcttg ctactaaatc
     3721 tgttgctgtc gccgttagtg ccagtgtgac cgggtttcct aaaagctttc ggatttttcc
     3781 taactgttga tattctggac ggaaatcaac tccccattga gacacgcaat gagcctcatc
     3841 tactacatat aacgcaatat tttgtttttt cagatgctct aaaagagaag gattagtcaa
     3901 catttcggga ctcaaaaaca aaaatttata ttgcgataga tgacggagga cgtatcttct
     3961 ttcagattgc gaaagtaaac tattgaaagc agctactcgt ttctcccctc gtttttgtaa
     4021 ttgcgtcact tgatcttcca ttagagaaag cagcggtgac acgatgatga ccagaccttc
     4081 cattaaaaac cccgtcaatt gatagcaaag gctttttccc gttcctgtag gcaaaatagc
     4141 gagagtatct tctccatcaa gcaacgcctg aatgatttct tcttggccgg gacgaaatga
     4201 atcaaatc
//
LOCUS       DAKBVG010000196         4202 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000196 DAKBVG010000000
VERSION     DAKBVG010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4202
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.196"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(140..1342)
                     /locus_tag="OUD09_002337"
     CDS             complement(140..1342)
                     /locus_tag="OUD09_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCT9693571.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(1345..1539)
                     /locus_tag="OUD09_002338"
     CDS             complement(1345..1539)
                     /locus_tag="OUD09_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HCT9693572.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(1586..2434)
                     /locus_tag="OUD09_002339"
     CDS             complement(1586..2434)
                     /locus_tag="OUD09_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HCT9693573.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(2639..2989)
                     /locus_tag="OUD09_002340"
     CDS             complement(2639..2989)
                     /locus_tag="OUD09_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693574.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            3584..4030
                     /locus_tag="OUD09_002341"
     CDS             3584..4030
                     /locus_tag="OUD09_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HCT9693575.1"
                     /translation="MKDYVVQITKEVLEESKTLNKLSSKAKEEAVTILSSGIIDYIEM
                     SGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSYLIGKLINILGTDEDTELFLSSLS
                     MIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREKTPDEFYDWFEK"
BASE COUNT         1233 a          724 c          782 g         1463 t
ORIGIN      
        1 aaaaacgttg atttatcggc attccccatc catattatat ttacgtctta ttcccactca
       61 atatgagtag cctcgtaaag aagctattat atcagtgctt tttcagccta ttcctcattt
      121 tggtagggaa atggtagggt tagattttta ctgcttgatt tccattaaag tttgcaactt
      181 tagattttgt tgcacaggta aatttaatgt ttcctgtcat ttgttctgca attccttcat
      241 cattacggct ccatagatga gcgtagtgct ttaaagtgat ttcagggcta gaatggccta
      301 accgttgcga cacgaccaga atgtctgcgt taaactcatt gattaggtag ctaacatgag
      361 aatgacgtaa tccttttcct tgtattaggg gtaccttagc tagtttggca taacgctgaa
      421 taattcgttg aacagttgaa cgatagagtg ggaggtcagt gtagctaaga ataaattgtt
      481 ctacaccatg atttttttga ataactttcc acttttttag cacttcaatt gtatcatcgt
      541 ccagagaaat gatccgcaag ccgctttcag tttttgtgta aggtttccga ataaaatctt
      601 tttgatttgt catttccaag gtgtgatgaa ccctcaactt tttacgtttg aagtccacgt
      661 cattccaatt aagtgctaat ccttcgctta cacgaacacc agtcatgtaa tatagccaga
      721 tcattaaaaa acacatatgt tcgtagaaat catctttata aatttttgcc aaaacttctt
      781 caaattcttc ttttgtccag taaggaacga ttgattttcc ttttggaatt gcttttgttc
      841 ttttggaaat attctcattc agaaattgca gggtgaccgc atagtctaat gatttacgaa
      901 acataccgta cataagtgaa ctgtatgctt gtgagtatcc gctatcgttc aataaccaaa
      961 ttcgataatt ttcacaatct cgcacattga tttcacgaag cttcttatca ccgaaaaatt
     1021 cagtaatctg aatcaatcca cactcacgag aactccaagt acttttttgt acgctagctt
     1081 tgtagtgtgg aacgtaagac tgctccataa attctttgta agttaaatca taattcgcaa
     1141 atccatttac aaccatgtat tcatttttta tacgcatgac ttctttgtgt gcttcacgag
     1201 ctgatgagaa tcgttttcct agagaatctt ttgttccttt cttctggatc ctctttccag
     1261 tcacttgatc tgttcctaac tcaactagat aagaaaagcg accattttta tcttgataca
     1321 cacaagggta ttttgttttc atagttattc ctcctgtgaa agaggaacac ctataatttc
     1381 ttcaattact tgtcggggta ctaccccaac tcttttatta ttataatatg gatatccctg
     1441 atttaccatt cttgtttttg cttgtcggat tattgtggta gcttgatgtg ttttaaatcc
     1501 tagagcctct aaatcttttc gacaaattgt atttttcatt gtataaactc actttcaaag
     1561 aaaacactat cattgggtaa tagtgttatt tctttatttt tgtataatga acggtacttt
     1621 cttgaaaatc catcacttgt tcaggacttc ccaactcatt taaagctaaa gcattgtaaa
     1681 aaaatggacg tctaactata tattcgcttg tatatagtat atcttgattc gtggaatcat
     1741 agggtttctt taccatcttt ttggtgacag ttggtagttt gagattccgg gacttgaaaa
     1801 aagctttttt cttatgctct ttcaattcca gctctttatc gaaatatttg ctgatatagc
     1861 gcccgcgatt ttcgatactg tctacatcga tcttgttgat cttaacgaaa ccctgacccc
     1921 aaatcgtagt taaccggtta aatgagataa aggggaaatc aaacaagata atatggtagt
     1981 ggattgcacc gcgttgctgc ttttcccacg tggcgagata ctttaaattt gcttttttag
     2041 ttttatagag gtcataattc agtcgtttga taaatttttt aaattcatta tttgcatact
     2101 caatgtcttg aatgttttct ttgaaagtga gagttaaaaa tttcgtttga ttgtcaaaat
     2161 tcgtgtctac aaggcgggca atctcaaatc gcatactttg ataatgtttc tgtttgcgtt
     2221 ttaaactgtc atattgtcct tgagcggata attcatcata gttttttctt ttttcaagag
     2281 taatatcatt ttcataaatc caagctaatt tttgttttcg ttccagattt acttcgtttt
     2341 cgatcgtttt acgtttggga gtaatgattg cttcataatc ataaatttca atatacgtgg
     2401 gagtttcaat gattttctga ttataagctt gcaaaaatca tcactccttg ttgcgtattc
     2461 gattgtcaaa gaaccagtca ttttctcctg atgttgatct ataaatcaag ttaaggggga
     2521 aggcctgcgt agcagccctt ctagcctatg gggtgttggg cggtcttaac ggacagaaac
     2581 ggctctgtgg gcgctgcccc gccgtttcgt cctaaccacc caacactcca tagtatagtt
     2641 agttttgtgt agtggtacct tgttttctgg gaatcaaagt tgccgtatca gcggttgccg
     2701 ataacccata actgacaaat tgtccattcg agcgtcccca aggtacaatc accaaattgg
     2761 taaatgtaat tatggctaaa ccttgttggg taagttcttc cggcgtgatc acaggatgtt
     2821 cagatttgac tttgacactg attttttcgt agcgaagttt tggtaggata cattcgtagc
     2881 tccaacctag aatttcgccc tccttttgcc agcgagtgac attgacgact tggtattctt
     2941 tttcgagtgt ttcttttggt aaactgagat cggtaatttt tattggcatc acaattctcc
     3001 ttttttatac gaaagtttat acgatttgat taaaaaatat agatggccat ctacgacttg
     3061 tattatatac gagaacttat acgtagtcaa gagattttct aaaaaaatat atacgatttt
     3121 ttatacgagt gttataatga aaaaaatgat aaggaggaat ttgaatatgt ttggtttagt
     3181 acaacctaat aaagtgaagg tgggccaacg aatcaaagaa ataaaagacg gaatgtctct
     3241 ctcttttaca gaattaggga atcgattagg tttaaaaaag ccgacgatca gttcctacgt
     3301 tcaaggctat gcgttggctc ctaagagtgt gatcaaccaa ttatctagta tcagcggaaa
     3361 acctgtcggc tggttttatt ttgggcatgt ggaagaatat atccgagagt atttgtttct
     3421 cattggtcag aaaaaaatag tggacgatta tcctgaaatc attgagaaaa taaagcaaga
     3481 attttatacg ggcgagttca aaaatcctgc ttgggagaat gagctaggct atcctttgga
     3541 ggaatttatc caagaatgtt ttgtagaata ttcgagtgaa atcatgaagg actatgtcgt
     3601 acagattacg aaagaggtac ttgaagaatc caaaacgcta aacaaactat catcaaaagc
     3661 aaaagaagaa gctgtcacga ttctttcatc tgggatcatt gactatatcg agatgagtgg
     3721 tgagatcaag tatggagaga aagatcgcat tgcgaaaatg gtgaaagaat caataaaaac
     3781 ctttgatgtg aaacaagacc tgacatttaa tgattcctat ttgattggaa agttgatcaa
     3841 tattcttggt acagatgaag atacggagct gtttctttct tctttatcaa tgatatttac
     3901 tgataagcat ttttccactc gatttggtgg ggaagaatta gtcgaaatat ttcaatcgat
     3961 gcgaccagcg ttaatcaaac tctatcggga gaaaacacca gatgaatttt atgattggtt
     4021 tgagaagtaa aataagaaaa gtggcttaga ttacaagttg gtgaaaaaat ttttttcagc
     4081 ataacatcag taatctataa aggggttgtt gattatactg tagaagttaa gtactcaaga
     4141 gattttttgt acgtaaacaa agcaaaaaag ggtgcagttt cactagaaaa tgtactcttt
     4201 tt
//
LOCUS       DAKBVG010000197         4166 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000197 DAKBVG010000000
VERSION     DAKBVG010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4166
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.197"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(37..1545)
                     /locus_tag="OUD09_002342"
     CDS             complement(37..1545)
                     /locus_tag="OUD09_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HCT9693576.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            1704..1895
                     /locus_tag="OUD09_002343"
     CDS             1704..1895
                     /locus_tag="OUD09_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693577.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            2098..3015
                     /locus_tag="OUD09_002344"
     CDS             2098..3015
                     /locus_tag="OUD09_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HCT9693578.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            3008..4081
                     /locus_tag="OUD09_002345"
     CDS             3008..4081
                     /locus_tag="OUD09_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693579.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
BASE COUNT         1404 a          763 c          803 g         1196 t
ORIGIN      
        1 atagtttcgt tttcaacatt tataagtaaa ctttttctat tggtaatata gattttcgga
       61 agggtagaca ggtgcgcagg taatatcgat tcctagtttt ttcaatgttt gttcatcatt
      121 tttattgacc atcacagtag agtgggcttg tgctccctca agttcagcta gtttatcata
      181 agcaacttga gcgattggat tcgttactgc actgattgat aaagcgatca agacttcatt
      241 gatgcttagt gcatccaaag aggaatgaag tgtctttgat ttcaatcctt gtattgtttc
      301 aagaactaat ggtgacaata aaggaatgtc atctgcgata tgggctaaat gttttactgc
      361 atttaagata actgccgctg acgcatccat caatgaactt gtacgcccgg taatgatgac
      421 ttcatctggt aattcgattg caatcacagc agaaggatct tttgtaggac tttgcgcttg
      481 aatcttatct gcatagtcat gggcagggac taccacgttt cggtcttcag gacgtaaacc
      541 gacttcttcc ataatcagtt tgattcgatt aactgcttct tcgtcgatca atcctttttt
      601 gaagtcgcat tctgtttgga aataacgacg gatgatttct tgtttagagg cttcttggac
      661 aacggtatca tccacgatcc caaatcctac acgattcaca cccatgtcag taggagattg
      721 ataaacagat tctttaccag taattttttc aataatacgt ttgattacag gaaatgtctc
      781 aacatcccga ttatagttca cagctacttt attgtaggca tcaaagtgga aggaatcgat
      841 catattgaca tcttttagat cgactgtggc tgcttcataa gcaatgttca gcggatgttt
      901 caacggaaca ttccatactg ggaaagtttc gaacttggag taaccagctg catgtccttt
      961 ttggctttca tgatacagtt ggttcaagga agtagcgagc tttccacttc ctgggccagg
     1021 agctgtcact acaacgattg gtttagttgt ttcaatatac tcattttttc caaaaccatc
     1081 ttctgagacg attttttcta cgttgactgg atagtcttcg atttctgagt gtttatagac
     1141 cttgatgttt cgtctttcta gtttattgat aaagactttc gtggcaggct gtccactata
     1201 gcgagtgata acgacactgt tgattaataa gccataacca cgtaattcat cgattaaccg
     1261 caagatatcc atatcataag taatcccata atcaccacgg atcttattac gttcaatatc
     1321 tccagcatag acgcaaatca gtatttcagc ttgatctttc aatttttgca gcaatttgat
     1381 tttcgcatct tcttcaaatc ctggaagtac acgtttcgca tgcttgtcgt caactagctt
     1441 cccaccaaat tctaaatata atttgtcata gtggttcact cgttccaaaa tatacgctga
     1501 ctgctcttca atatattttt ctgaatcaaa accaattttt ttcactaggt tccctcctta
     1561 agtactaaag cattttttca tttttttgag gattcgtcaa ctggaagagc gaaattcctc
     1621 aaaaaaacaa agaaaatggt ttaaaccaac ttgtctagat agttattcat tggtatgata
     1681 gtttttgata aggaaggtgc tacatgaaaa aaagacagaa aaagaaaaac gcatataaac
     1741 actatatccg ttcgattttt accggttatg aaagaatgct tgaagatccc gaattggaac
     1801 aattgacgtt tacttatcta aacgaagaaa cgcagctaac tcgtgatgag catcagcgca
     1861 ttcatttcac aacaagagat ctgcctagta aataaaaaat aagataaagg tcatcggatt
     1921 cgattctgtc ctatcagatg gaaagactag ttgatcgaaa aaaaggctgt accaaatttt
     1981 ggtgcagcct gttttatgga gcattgcccg ctctttttta taacaatttt ttacagaaaa
     2041 tggtactatt gaagtattaa taagtgaaaa acagatgagt aagaggaggc aagtagtatg
     2101 aaagagccag atacaaatgc aaattatgag ccagacgaac tagaggagac gaatgcggat
     2161 caggatacag gacaagaatc gtcttctttt tacgatatca aagaaaaagc aagcatgaat
     2221 ctttcccaat tattagaaga cttacaggct tttgaaaaag ctgtccgcga agaagatatc
     2281 cctgagatct accgtttata caatggccga ttgaatgaag aattaaaaag aagctcaaat
     2341 gaaaatcacg agatcgatca attgctgatg cgtaagatcc atgatcgttt tttacaggaa
     2401 tttcctttta tggaacaagt agaaaacatc tcgccaacga tgagctatta caaaatcggc
     2461 acttattacc atgatcggcc cacggtcggt attgatgcaa gtctgccaga aatttttgtt
     2521 ttgcctaaga tcgatgaaga gtgggagaaa tattcccaac cggagtcaac ggactatgac
     2581 aaaaaaatca atgagctaga cgcaaaggtg atcactgctc agacagaaat tgaacgttta
     2641 gatgaacaaa taaaagaaat caatcgacaa atgactgatt tgaacgacaa taaaggattc
     2701 ttgaatcgta aaaaaatcga ggaagaaatt caagaattag agaagaagaa gcaagtacta
     2761 tctaatgaaa aattaggctg gcttccttat attgagacgc ctgaaacaat ccagcagcaa
     2821 aaagaagcat tgaagcaaga agcaagagca gaccagcttc gtgcagccat cgtagaaaaa
     2881 gaacaaaggc aaatcagacg ctatttcggt agcaaagagg gattcggtca agcaatccat
     2941 gaattcttga tgaattatct tggcaatgag aatcctaata atcaatctaa tgaaggaggt
     3001 tccgagtatg aatgaacacg atgaagcaga atggacaaaa aatgaagaac aaacagaaga
     3061 tcatgaagaa acgaacactt cattggaaga acaaacaagt aatcctctgg atgacacaga
     3121 agaaatccct atgctagagg aaacaaaaat acacgaaaaa gaaaaagtaa ctccgacgaa
     3181 tacgaatact gcagaaaaca cccatgctta cgaaataact gaaaatcccg ggtatttaga
     3241 aggaaaagag caagatcatc aagatatcgt cagtttgatt gaaacagcgg aagcagaatt
     3301 agcggattta cgtatgagaa aagatttgat cgaatcagat tttccacaat tattagaatc
     3361 atatgatcgt tttatattga atgaagaacc aatctccacg atcggaaaga caagcctgat
     3421 cgaatacttc acgtatgaat tactgaaacc gttgaatgat attggtctgg aacggtcaaa
     3481 ttcagcttat gacacatgga aaacaagaca tttttcgatc aattatacgt tgaatggaca
     3541 aaacgaatta gtcttttcct ttgtattacc gaccatcgat caacgatacc aagtggaatc
     3601 gatgagtctc ctaaacgtat caccagagac aatggaaatc aatgtccaag atgaccgggt
     3661 gctttctttg atccgacact ggtcagtgga tcgagtattc tctgcagggc agatcacgat
     3721 attcaatcac aaattgaatc aattattagc ccatgcaaga acacttggtt ttaccgttaa
     3781 tcagacatta ctagacaata cgaagccact tcgtttgaat cttcagagcg aatttgaatt
     3841 gacgaatgaa gtattggatg atatttttat cgtggcaatg aaaaatccat cctacgatat
     3901 ggaaaagatc aacgaaacta cttatcaagt acgattagat aaaggacagt ctttaacgat
     3961 aacaacaaac gaaaaagatc aaacagtctt gtcgatctca tctggagact acccacgatc
     4021 tgtcattgat ttctttatca attacgaatt cttggtccca ttaatggtga gaaagtcctg
     4081 attggataag ttggattacc agatgtcatt cagataattc tgttccaaag gacttgcggc
     4141 agttatccag aataattgtc acaagt
//
LOCUS       DAKBVG010000198         4110 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000198 DAKBVG010000000
VERSION     DAKBVG010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4110
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.198"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            <1..1187
                     /gene="tet"
                     /locus_tag="OUD09_002346"
     CDS             <1..1187
                     /gene="tet"
                     /locus_tag="OUD09_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Tet(L)/Tet(K)/Tet(45) family tetracycline efflux
                     MFS transporter"
                     /protein_id="HCT9693580.1"
                     /translation="GTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFFSLLIM
                     ARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGMIAHYI
                     HWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTTSYSIS
                     FLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVSMVPYM
                     MKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSVSFLTA
                     SFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTSFLSEG
                     TGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVYKHSQR
                     DF"
     gene            1751..3013
                     /locus_tag="OUD09_002347"
     CDS             1751..3013
                     /locus_tag="OUD09_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HCT9693581.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            3258..4110
                     /locus_tag="OUD09_002348"
                     /pseudo
     CDS             3258..4110
                     /locus_tag="OUD09_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336291.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
BASE COUNT         1431 a          561 c          854 g         1264 t
ORIGIN      
        1 ttggaacagc tgtatatgga aagctatctg atcaattagg catcaaaagg ttactcctat
       61 ttggaattat aataaattgt ttcgggtcgg taattgggtt tgttggccat tctttctttt
      121 ccttacttat tatggctcgt tttattcaag gggctggtgc agctgcattt ccagcactcg
      181 taatggttgt agttgcgcgc tatattccaa aggaaaatag gggtaaagca tttggtctta
      241 ttggatcgat agtagccatg ggagaaggag tcggtccagc gattggtgga atgatagccc
      301 attatattca ttggtcctat cttctactca ttcctatgat aacaattatc actgttccgt
      361 ttcttatgaa attattaaag aaagaagtaa ggataaaagg tcattttgat atcaaaggaa
      421 ttatactaat gtctgtaggc attgtatttt ttatgttgtt tacaacatca tatagcattt
      481 cttttcttat cgttagcgtg ctgtcattcc tgatatttgt aaaacatatc aggaaagtaa
      541 cagatccttt tgttgatccc ggattaggga aaaatatacc ttttatgatt ggagttcttt
      601 gtgggggaat tatatttgga acagtagcag ggtttgtctc tatggttcct tatatgatga
      661 aagatgttca ccagctaagt actgccgaaa tcggaagtgt aattattttc cctggaacaa
      721 tgagtgtcat tattttcggc tacattggtg ggatacttgt tgatagaaga ggtcctttat
      781 acgtgttaaa catcggagtt acatttcttt ctgttagctt tttaactgct tcctttcttt
      841 tagaaacaac atcatggttc atgacaatta taatcgtatt tgttttaggt gggctttcgt
      901 tcaccaaaac agttatatca acaattgttt caagtagctt gaaacagcag gaagctggtg
      961 ctggaatgag tttgcttaac tttaccagct ttttatcaga gggaacaggt attgcaattg
     1021 taggtggttt attatccata cccttacttg atcaaaggtt gttacctatg gaagttgatc
     1081 agtcaactta tctgtatagt aatttgttat tacttttttc aggaatcatt gtcattagtt
     1141 ggctggttac cttgaatgta tataaacatt ctcaaaggga tttctaaatc gttaagggat
     1201 caactttggg agagagttca aaattgatcc tttttttata acaggaattc aaatcttttt
     1261 gttccattaa agggcgcgat tgctgaataa aagatacgag agacctctct tgtatctttt
     1321 ttattttgag tggttttgtc cgttacacta gaaaaccgaa agacaataaa aattttattc
     1381 ttgctgagtc tggctttcgg taagctagac aaaacggaca aaataaaaat tggcaagggt
     1441 ttaaaggtgg agattttttg agtgatcttc tcaaaaaata ctacctgtcc cttgctgatt
     1501 tttaaacgag cacgagagca aaacccccct ttgctgaggt ggcagagggc aggttttttt
     1561 gtttcttttt tctcgtaaaa aaaagaaagg tcttaaaggt tttatggttt tggtcggcac
     1621 tgccgacagc ctcgcagagc acacacttta tgaatataaa gtatagtgtg ttatacttta
     1681 cttggaagtg gttgccggaa agagcgaaaa tgcctcacat ttgtgccacc taaaaaggag
     1741 cgatttacat atgagttatg cagtttgtag aatgcaaaaa gtgaaatcag ctggactaaa
     1801 aggcatgcaa tttcataatc aaagagagcg aaaaagtaga acgaatgatg atattgacca
     1861 tgagcgaaca cgtgaaaatt atgatttgaa aaatgataaa aatattgatt acaacgaacg
     1921 tgtcaaagaa attattgaat cacaaaaaac aggtacaaga aaaacgagga aagatgctgt
     1981 tcttgtaaat gagttgctag taacatctga ccgagatttt tttgagcaac tggatccagg
     2041 agaacaaaaa cgattttttg aggaaagtta taaattattt tccgaacgat atggcaagca
     2101 aaatattgct tatgcaacag ttcataatga tgagcaaacc cctcacatgc atttaggtgt
     2161 tgtgcctatg cgtgatggaa aactgcaagg aaaaaatgtg tttaatcgtc aagaactgtt
     2221 atggctacaa gataaattcc ccgagcatat gaaaaaacag ggttttgagt tgaagcgtgg
     2281 tgaacgtggc tctgaccgta aacatattga gacagctaaa tttaaaaaac aaactttgga
     2341 aaaagagatt gattttctag aaaaaaattt agcagttaaa aaagatgaat ggactgctta
     2401 tagcgataaa gttaaatcag atttagaagt accagcgaaa cgacacatga aaagtgttga
     2461 agtgccaacg ggtgaaaagt ccatgtttgg tttgggaaaa gaaataatga aaacagaaaa
     2521 gaaaccaacc aaaaatgttg ttatatcgga gcgtgattat aaaaacttag tgactgctgc
     2581 gagagataac gataggttaa aacagcatgt tagaaatctc atgagtactg atatggcgag
     2641 agaatataaa aaattaagta aagaacatgg gcaagttaaa gaaaaatata gtggtcttgt
     2701 agagcgattt aatgaaaatg taaatgatta taatgagttg cttgaagaaa acaagtcttt
     2761 aaagtctaaa ataagcgatt taaagcgtga tgtgagttta atctatgaaa gcactaagga
     2821 attccttaag gaacgtacag acggcttaaa agcctttaaa aacgttttta aggggtttgt
     2881 agacaaggta aaggataaaa cagcacaatt ccaagaaaaa cacgatttag aacctaaaaa
     2941 gaacgaattt gaactaactc ataaccgaga ggtaaaaaaa gaacgaagtc gagatcaggg
     3001 aatgagttta taaaataaaa aaagcacctg aaaaggtgtc tttttttgat ggttttgaac
     3061 ttgttctttc ttatcttgat atatatagaa ataacgtcat ttttatttta gttgctgaaa
     3121 ggtgcgttga agtgttggta tgtatgtgtt ttaaagtatt gaaaaccctt aaaattggtt
     3181 gcacagaaaa accccatctg ttaaagttat aagtgaccaa acaaataact aaatagatgg
     3241 gggtttcttt taatattatg tgtcctaata gtagcattta ttcagatgaa aaatcaaggg
     3301 ttttagtgga caagacaaaa agtggaaaag tgagaccatg gagagaaaag aaaatcgcta
     3361 atgttgatta ctttgaactt ctgccatatt cttgaattta aaaaggctga aagagtaaaa
     3421 gattgtgctg aaatattaga gtataaacaa aatcgtgaaa caggcgaaag aaagttgtat
     3481 cgagtgtggt tttgtaaatc caggctttgt ccaatgtgca actggaggag agcaatgaaa
     3541 catggcattc agtcacaaaa ggttgttgct gaagttatta aacaaaagcc aacagttcgt
     3601 tggttgtttc tcacattaac agttaaaaat gtttatgatg gcgaagaatt aaataagagt
     3661 ttgtcagata tggctcaaag atttcgccga atgatgcaat ataaaaaaat taataaaaat
     3721 cttgttggtt ttatgcgtgc aacggaagtg acaataaata ataaagataa ttcttataat
     3781 cagcacatgc atgtattggt atgtgtggaa ccaacttatt ttaagaatac agaaaactac
     3841 gtgaatcaaa aacaatggat tcaattttgg aaaaaggcaa tgaaattaga ctatgatcca
     3901 aatgtaaaag ttcaaatgat tcgaccgaaa aataaatata aatcggatat acaatcggca
     3961 attgacgaaa ctgcaaaata tcctgtaaag gatacggatt ttatgaccga tgatgaagaa
     4021 aagaatttga aacgtttgtc tgatttggag gaaggtttac accgtaaaag gttaatctcc
     4081 tatggtggtt tgttaaaaga aatacataaa
//
LOCUS       DAKBVG010000199         3941 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000199 DAKBVG010000000
VERSION     DAKBVG010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3941
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.199"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            95..1054
                     /locus_tag="OUD09_002349"
     CDS             95..1054
                     /locus_tag="OUD09_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS1252 family transposase"
                     /protein_id="HCT9693582.1"
                     /translation="MTYTHLTTDELVMIESYYHQNIKIVKIAEYLKRSRTPIYNVINF
                     LKEGHTALEYYQQYKENKKRCGRRNIVLPKKQQSYIKEKIAQGWTPDVIIGRKEKPIS
                     CSVRTLYRQFKEKVFDESTLPMKGKRKPNGHQERRGKQAFKRNIVERETDYPTFKEEF
                     GHIEGDTIVGIHHKSAVITLVERLSKIIITLKPEGRKAIDIENTLNHWFGSIPRHLFK
                     SITFDCGKEFSNWKALSNQHDIAIYFADPGTPSQRALNENSNGLLRKDGLPKNMDFNQ
                     VDQAFISSVANKRNNIPRKSLDYQTPLEVFMSYMNEDILSSLI"
     gene            complement(1487..2059)
                     /locus_tag="OUD09_002350"
     CDS             complement(1487..2059)
                     /locus_tag="OUD09_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCT9693583.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2075..2680)
                     /locus_tag="OUD09_002351"
     CDS             complement(2075..2680)
                     /locus_tag="OUD09_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HCT9693584.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            complement(2767..3588)
                     /locus_tag="OUD09_002352"
     CDS             complement(2767..3588)
                     /locus_tag="OUD09_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCT9693585.1"
                     /translation="MYQFLKTGATILNYYQQYKENKKRCGRRSIALPEEQKEYVQKRV
                     VQGWTPDVIVGRSEMKLDCSVRTLYRMFQTGAFNVRDLPMKGKRKPNGHQEKRGKQAF
                     RRSIHERIKEHPEFENEFGHLEGDTIVGRKHKSAVITLVERLSKVIITLKPAGRQAMD
                     IENRINHWFQTVPKHLFKSITFDCGKEFSNWKAMGNQNDIDIYFADPGTPSQRALNEH
                     SNGLLRRDGLVKAMDFNEVSESFIEYRKHFLYSILSSFKFSIDYIYCEFCRGSET"
BASE COUNT         1220 a          781 c          668 g         1272 t
ORIGIN      
        1 gccgattgta aaattaagct agacaaataa ataagccatt cgtgctacac tcaaaatgta
       61 attccgacta aagaaaacat aggagtgatc acgaatgacc tacacccatc ttaccacgga
      121 tgaactggtg atgatagagt cttactacca ccaaaacatc aaaattgtga aaattgctga
      181 gtacctgaaa cgatctcgaa caccgattta taacgtcatt aatttcttga aagaaggcca
      241 tacggccctc gaatactacc aacagtataa agaaaataag aaacggtgtg gcagacgaaa
      301 catcgtcttg ccaaagaaac aacagtccta cattaaagaa aaaattgccc aaggctggac
      361 gccagacgtt atcattgggc gtaaagagaa accaatcagc tgttccgtaa gaacccttta
      421 tcgccagttc aaggaaaagg tgttcgatga atcgactttg cccatgaagg gtaagcggaa
      481 accaaatgga catcaagaac gccgaggaaa acaggctttt aaacggaata tcgttgaaag
      541 agaaactgat tacccgacat ttaaagaaga gttcggccat atcgaaggcg ataccattgt
      601 aggcattcat cataagagtg ctgtcataac tttggtagaa cgtctatcga aaatcatcat
      661 caccttgaag cccgaggggc gcaaggcgat cgatattgag aatactctga accactggtt
      721 tggatcgatt cccagacatt tatttaaatc cattaccttt gattgtggga aagaattctc
      781 taattggaag gctttaagta accaacatga catcgctatt tatttcgctg atccagggac
      841 tccttcgcaa cgagcactga atgaaaattc caatggcttg ctgcgaaaag atggtctgcc
      901 aaaaaacatg gattttaatc aagtcgacca agcttttatc tcttcagtgg cgaataagag
      961 aaacaatatt ccacgaaagt ccttggatta tcaaacaccc ctggaagtat ttatgagtta
     1021 catgaatgaa gatattttgt ctagcttaat ttgacaaatc aaattattta aaactttgca
     1081 acagaacctt agcagcagtg ctgattttta tgatagttat ttggttctta aatgaaagat
     1141 ctaaagacaa tcaacaccgt agggatatgg agataaaatc aatagagtca atagattatt
     1201 tatcgataat aacactaaaa aagccacgtt cctaaacgtg acttacgctg aacgatatgt
     1261 gttgtttggt agacgaacaa atcgttcatt ataaatatat gatggtatcc taatatagta
     1321 agcgtggcta gcataccttt aattacttta ccttttacag ttaccataca cattttttga
     1381 gcagaaatca agtaaataaa aaaatattat atttcacaac atgctaatag actcatatct
     1441 agaacgcatt aagtatttac ctgtctggtt cgctgagact tcttttttaa ctttcttcaa
     1501 ttttcttctt tattctaaaa agcgtactgc gactgaaacc ggttatagct tcaacttcct
     1561 tatacgattt acctgaagta agcagttcat atgcgtgcct cttcttcggt gtgattgttg
     1621 ctttcggacg tccttctctg aaattaggat tattctttct tgcaaacatt tttccttctt
     1681 gggtacgttc cacaatcata tcacgttcca tttctgctac actcaacaac gtccgtgtca
     1741 ccattcgccc catggacgta ttttcaatcg ttcctagatt caaaaccttg atcgttacgt
     1801 tatcatcgag taaaggttca atgatctcta atgcttctct cgtgttacga gcaaatcgat
     1861 caagcttcgt aacaactatt tcatctccag gcttcagacc agccaacagc tgattgaatt
     1921 ttggccgatc ccgttttgta cccgtaaatt tttctgaaaa aatattcttc ttctctacgc
     1981 cgttctcaag caactgttga atttgttctt ttgtactttg agaagcggtt gagacacgtg
     2041 catatccata tttcgtcatt tggtccacct acttttacaa atcatctatc gtatactcag
     2101 aatacccttc atagaaatag ctgacatcga ttcctttcat atataactcg cgatcgtcaa
     2161 ttttatcagt cagagcattg gaaattagat agcgtatttc caaatcgttt atgggacttc
     2221 gctccattgc agaaagatag tctgctttat cgattaagtt ccaatctaca actttttgca
     2281 attcttcctt taaaatgaga tccagccaaa ttctagtact tcggccattt ccttctcgaa
     2341 atgggtgagc gatattcatc tccacatatt tcttgataat ctcttcaaaa gaagtttgtg
     2401 gcatctgatc aatatgctgt aacgaatgtt ctaaatacat aactggtgca aatcgaaaat
     2461 ttccttttgc aatattgacc gatcggattt ttccggcaaa atcgtaaata tccgaaaata
     2521 gaaattcatg aatttcagat aaacctttaa atgtaccaac ttcgatttca tttatttttc
     2581 cacaatcgta caattctttc gctttttgtt tactgagttt ttcttcaacc ttagccaact
     2641 caacttgatt ggttaatccc aatttattct ctaaaatcat gcgaaatctc cttaacttta
     2701 tgacataagt ttatgaaaca ctctcaaccc cattatatcg cgattttgtt tatgtgtcaa
     2761 aaactattaa gtttctgacc ctctacaaaa ttcacaatag atataatcta ttgaaaattt
     2821 aaaagaagac aaaatagagt acagaaaatg ttttctgtac tctataaatg attctgatac
     2881 ttcattgaag tccatggctt taactaatcc atcacgacgt aataatccat ttgaatgttc
     2941 atttagagcg cgttgtgacg gggtgcctgg atccgcaaaa taaatatcaa tatcattttg
     3001 atttcccata gctttccaat ttgaaaattc tttcccacag tcaaacgtaa ttgatttaaa
     3061 cagatgttta ggaaccgttt gaaaccaatg attaattcga ttttcaatgt ccattgcctg
     3121 tctaccggct ggcttcaatg taataatcac ttttgacaaa cgttccacca atgtaataac
     3181 agcacttttg tgttttcgac cgacaatagt gtctccttca agatggccaa actcgttttc
     3241 aaattctgga tgctccttga tgcgctcatg tatactgcgc cgaaaagctt gtttcccacg
     3301 tttttcttgg tgtccatttg gttttcgttt gcctttcata ggcaaatcac gcacattaaa
     3361 tgcgccagtt tgaaacattc gatacagtgt acggactgaa caatcaagtt tcatttccga
     3421 gcggccgaca atcacatccg gtgtccaccc ttgaacaacc ctcttttgaa catattcttt
     3481 ttgttcctct ggtaaagcaa tcgagcgtct accgcagcgt tttttgtttt ctttgtattg
     3541 ttgatagtag ttcagtatcg tagcaccagt tttcaaaaat tgatacacgt tatgaatcgt
     3601 ttgtcacgca cgtcctaagc attgcgcagt tttggcaacg gattgattat ttttgaaata
     3661 agactctatg attaccagtt cgtctgttgt aagatgggtg taggtcattt gtactcactc
     3721 cttaataatt ctttggtcgg aactattttg agtgtagcac aaatgatctt tttagttgtc
     3781 tagcttaatt ttacaatcga cgaaaagaat actatgatga gattagtttg aaatttcatt
     3841 ttgatgagta gatattagga actgattgat agaacgaata agttatattg atattttgta
     3901 tttgcagtat ttgaaaaagt tgaagatgat aaggaaaata a
//
LOCUS       DAKBVG010000200         3896 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000200 DAKBVG010000000
VERSION     DAKBVG010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3896
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.200"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            172..516
                     /locus_tag="OUD09_002353"
     CDS             172..516
                     /locus_tag="OUD09_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCT9693586.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            517..936
                     /locus_tag="OUD09_002354"
     CDS             517..936
                     /locus_tag="OUD09_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HCT9693587.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1028..1378)
                     /locus_tag="OUD09_002355"
     CDS             complement(1028..1378)
                     /locus_tag="OUD09_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693588.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1344..2675)
                     /locus_tag="OUD09_002356"
     CDS             complement(1344..2675)
                     /locus_tag="OUD09_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HCT9693589.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2930..3496
                     /locus_tag="OUD09_002357"
     CDS             2930..3496
                     /locus_tag="OUD09_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCT9693590.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3641..3728
                     /locus_tag="OUD09_002358"
     tRNA            3641..3728
                     /locus_tag="OUD09_002358"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3673..3675,aa:Ser,seq:cga)
BASE COUNT         1427 a          669 c          759 g         1041 t
ORIGIN      
        1 ataaaaaaag cgttagcaac tatgcttaaa aagcatatcg actgaataaa ctaagcattg
       61 gacaggctga aagactacat ttaaaatgaa atgcgaagaa acaatagaaa ataaagttgc
      121 cttagaacca tagttgaatc acctaacagt cgtgaaggga gaataagaga aatggcagaa
      181 aaacaaaccg caggacgaga tcaattagga gattttgcac caaaatttgc agaattgaat
      241 gatgatgttt tatttggaca agtatggtcc agagaagaat tatctgctca tcagcgtagt
      301 atcatcacca tatctgcatt aatttcagga ggaaattttg aacaattgcc agctcatctg
      361 aagattggca agcaaaatgg cattactagt gaagaaatag cagaaatcat tacccattta
      421 gctttttatg taggatggcc aaaagcttgg tcagctttta gtttagcaaa agaaatctat
      481 ttagaagaaa atagcgaaga taagaaagag gcgtaaatga cgacaaaaca cgaagaaata
      541 cacaatggcg tgatttttcc agcaggtgaa aaaaacgaag cttacgctca agtatttacc
      601 gggcaaagct atctgaatat gttagtgaat gatcccgaag tttctgttgg tgtaggtaat
      661 gtaacttttg aaccaggttg tcgcaatcac tggcatgtcc ataaagacgg ctatcaaatc
      721 ttacttgtga ccggaggaga aggctggtat caggaagaag gaaagccagc aagacgatta
      781 atggctggag atgtcgtcgt aacgcacaaa gggattaaac attggcatgg tgcgacaaaa
      841 gacagttggt ttgaacattt ggcgatcact gctggtaatt cagagttttt agaacctgta
      901 acagatgaag tgtatgaagg attggaaaat caataatgtc aagcttgaac agctcctgaa
      961 agtagatttt aatcgaactt tcaggagctg cttttttgat tcagtctttt ataaatagaa
     1021 aatagggtta ataataggaa tgagaagcac gtcgaaagat cttaatattg ataatcggct
     1081 gggcatacat gatttccacg tcatcaatca aactacctaa cagatgataa tcttcccgat
     1141 catgtcgggg attacctaat agttcttgat aatagatttc catactaggg tcccgaggat
     1201 tgttcagcaa atcagcaagt acatctaaat cttctggctg agtttctgcc gtaccctgta
     1261 tctctatgaa aagcccttcc tcagcaaatt ctactccaat acgtatttca gttatttcat
     1321 gggaatataa ataagtaaga agctcatcga tgattctttt tgataattct ttttccaagt
     1381 gtcttttttt cctttctgac agcggtaaag ttatctagta tcggtacaag gatacctgca
     1441 acgaaaccag ttgaaaaacc actgttatat agatttagtc ctccgtgcaa agtgctgacg
     1501 ttatggacaa gagcaatatg tagtacacca gctattacac cgtaaaaact gccgtaaaaa
     1561 ccgcttatgg gcgcgagacc tgtcccaaac acagctgcaa caagcatgcc agtactggtg
     1621 atctcatgaa aagttccaaa caaagaagca ataaatattc cgactagtat cggaaaactg
     1681 ttttttaaat gacaaccaaa agcactgaaa cccaccgcag ataaaatagc accgatgaca
     1741 ggtccattta actgaccgcc aaccagcaaa acataagaag acagcatcag accaactaag
     1801 cctatgttga tcaaagtagc accaatacca gaattagcaa tgaaatcagt tgtcaatttt
     1861 ccagatgaat cgaaaatttc tcgaatcccc aaaagccgaa aggaattata atagaaacca
     1921 attccgactg tcagcgaaaa aaagccaaaa ataatccaaa tgagaggaaa gtgatattca
     1981 ttgaaaacca atgtattttc gacgattgaa tagctgaata acctcaaaac agcagtaaaa
     2041 gtcatcgcaa tcaacccaga ggtgaatcca atattataaa gtgtgaatcc acgatggaaa
     2101 actaaagttt gagcagctaa gggcggaaga agtagaccaa ttgctatgcc agctaaattc
     2161 cccaataaaa tacctatcag taaaggaaaa cgcataccaa aagtgatata actaacgacc
     2221 ggtgataatg cgctaccaaa cagtccgatc atgatgtact gagaaaaagg tttattgaca
     2281 aatacagcgt atagatacac acctaaaatg attgagatag aattataaag gtttttgcca
     2341 aaaaaagaga aaccggtaac tgttagaatc gaagcaaata agggaccgtt taaaatcacc
     2401 ttgtgccgat aagctaaaag cacagaaagt aaggtcaaaa tccctacatt tatgaatgcg
     2461 cttccgaaac cgcctaaagc aaaataatct gtcaaaagat tgcttggcga ggtaagaata
     2521 gttagcaatc cactccaatt atcagtagga gtattagaaa gcaagccaat aaaaagaaaa
     2581 aaaacacctg taaaaccaag ataaaaatac tttcttttct gtgaaacgat tcgtttttga
     2641 ggatgaatat aaataacgtc agtttcgttt tccattacac accttctttt tcattttctt
     2701 taactataac ttgaaatgaa agatttggac aactatcatt taggtcaaaa ctaaaaatgt
     2761 aagggaaccc attgaattta accaaatttt tttaattttg caaaaaaaag ttaaagaaga
     2821 ctgaaaaaaa tttaaaatat aacgttttga gtaaaatgtt tgcaaaaaat gctaaaaata
     2881 gggtacaata aaacaacata tactgcagta aagaagtggg ggagttttaa tgagaagaaa
     2941 agtctatacc aaggatcaaa ttctaaaagc tgcttatgat gttgttgcaa aggaagggtt
     3001 taaaggattt actgcgagaa atattgctaa aaagatgggg atttcgacac agccgattta
     3061 tctggaattc aaaaacatgg aagatttaaa aaatacgttg ctggattcta tctttgaaca
     3121 actaaaagaa gatgttttcc ctattgaacg tactggtgat aaaatcattg atctcggttt
     3181 gaactatatc tactttgcaa aaaagcatca tagtctttac atggcattgt acattgatga
     3241 acatggggac ggaaagaaaa tgcatgattt ttcatacaat tatttccgtg aaatagttaa
     3301 agaagatgaa agatacaatc aattatcgga tgaatatgtc gatgctctgc atactggtac
     3361 atgggtagca gtgactggct tagcgtcatt gatgtcatca aacatcatgc atccaacaga
     3421 agaacaaatc attgcactga tcaaacggac aattgactcg attttagcat tagataatcc
     3481 tgaaataatc agatagtgtt ctatcagtat gaatttgatc ggtaagtccg ttggctgcaa
     3541 actgtcaatg gacttttttg ctaaaaaaag cgatttctgt tatttatcta ttagaaatag
     3601 gcttgacatt tcgtgaaaaa agtttaaaaa tagtacttac ggaaggttgg cagagtggta
     3661 atgcaccgga ctcgaaatcc ggcgaaccgg ctaataccgg cgcgcaggtt caaatcctgt
     3721 accttcctta tatcaacgat tagaggttgt gaagaagctg tcctgcatca agtaataaga
     3781 acggccaaac aaaaatagct tttcctattt tttcgtttga ttgggcttat taccgcagat
     3841 gcaagctttt gaacaccgtt tgttcggaat aaggggctgt gacaagactt ttgtca
//
LOCUS       DAKBVG010000201         3895 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000201 DAKBVG010000000
VERSION     DAKBVG010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.201"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(<1..369)
                     /locus_tag="OUD09_002359"
     CDS             complement(<1..369)
                     /locus_tag="OUD09_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693591.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNN"
     gene            636..2336
                     /locus_tag="OUD09_002360"
     CDS             636..2336
                     /locus_tag="OUD09_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HCT9693592.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            2306..2524
                     /locus_tag="OUD09_002361"
     CDS             2306..2524
                     /locus_tag="OUD09_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HCT9693593.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            2544..3434
                     /locus_tag="OUD09_002362"
     CDS             2544..3434
                     /locus_tag="OUD09_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCT9693594.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            3452..3736
                     /locus_tag="OUD09_002363"
     CDS             3452..3736
                     /locus_tag="OUD09_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693595.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
BASE COUNT         1360 a          644 c          795 g         1096 t
ORIGIN      
        1 attattctgt agtttataaa aaaacggcga atgaagatgt gttttcatat gaccaaaata
       61 taataattct gaaatttcta aaggtgtcaa ttctttcagc atcgttaaat cagtaaagtc
      121 gatccacttc aattcatcga atccacgcct gttacgagaa gaggaaaaga aacgctgaat
      181 attttctgta ccacggatta ctttcagccc tgtatgggat tcatattctc cagcagaagc
      241 agatggatca agcaagagca agttttgagg aaaatgcacg atactgtttg caaagtcagt
      301 agcagtcagc ccttttgtta aaacagcatt acttgtggta tcaatatgta cataaactga
      361 ctcagtcatc atgttcctcc tcttatttcc tcttaatctc ttttatttta caacatttgc
      421 attaaaaaag cttgtttctt ttattttaaa attccattac aaaatcacgt tttcattgaa
      481 aacactctca ttttcaatta atttttgtga tttttgtatt tttttattta aaggaggttt
      541 tatgaattgt tttcttatgg aatctacaaa gtcgaatacg agactcatga cctttttatg
      601 acgtactggt atacttgaag aaaaaggaga gcgcgatgga gataaaaaaa gtttactttt
      661 cacagaaaat gtcgctgtat tcgcctatcc agcaagcaaa acaacccctt ccattttttc
      721 aaagtcatca agaaagtaga gacgcttttt ggcgttctgc aaaaaagcaa tgtgctttta
      781 aagcagtatc gaaaaaatat accgtctatt ttactattgc taaaagtaga ggagatgtta
      841 tttttaataa tctgttgatt gaaggtgaaa cttttgaatg gaaaaaattt tttcggtata
      901 tggaagcttg cgctcgtttt tttattaaag aaaactgttg ttttttattt caatctcctc
      961 tatcagaaga atggttgcgt atttttcata aaaacggcta tcaaggaacc acacaattaa
     1021 ataaaaagct ggtctatcat acagcacttg ttttaggtgg aggaggagcg cacggtgctt
     1081 atcagattgg tgtgtggcag gcgctgaagg aacatgatat caactttgag attatcacag
     1141 gtacctcggt aggtgcgttg aacggcgcat tgattttaca aggagatatg aaaaaagctg
     1201 tgaatttatg gaaaaagctg tcgacaagac aagtgcttgc gttaccagaa atggctgcag
     1261 ttgaagacct tcgtgaacgc ttctatcgag aaagacgcca aatgacgaaa acagcactaa
     1321 ttgaaggcgg ggtttcttct gctccattgg aaaagatggt caaagacaat ttggatttgt
     1381 ccttactcaa acataatccc aaaatacgtt tatatacggt gtctactaaa ctgccagaac
     1441 tagcggaagt agtgactgat atccaacaga caaaaacaga agaaatacca gattggatac
     1501 tcgcctctgc ttcattttat cccgctatgg cttatcgaaa gatcggaaaa aataaatatg
     1561 cagacggtgg gtatcgcaac aaaataccta ttgatattgc gattaacaaa ggagcaacag
     1621 aggcatttgt cgtagatgtg caagggccag gcccagcgaa aaaaattcgt atgccagaca
     1681 tttttatcca ttggaaatgt caaacattgt ggacactagg cagctttctg ctttttgata
     1741 gccaaaggaa ccaattaaat ttacaattag gctatctgga aatgaaaaaa cgattaggct
     1801 attactatgg gaactggtat acatttcatt ctgttaaacc tgcaagaaca tactggcgag
     1861 gatttttgtc ttacttaaca aaagaaattc agttagaatt atcttttttc aaaagcataa
     1921 aattttggca aaaactgcgt aatttatata agaacagggt cgtaccagag acatgcggat
     1981 tggcgatgct agaactttta gcaaaaaaac attttttact gcccaatgaa atctatcaag
     2041 tagacatgat gattcaaatg atctgccaac aagatataaa aagtatgccg gatgatttat
     2101 tggcacaaat tggtcaatta agtactgaag aatggcgaag gtatcgaaaa taccagcaaa
     2161 aaatacaaaa tgagcgcacg aaaactgttt attttgatta tctgctacaa ggaaaacgca
     2221 aggatcgttt gcagcatgaa ttgcttaagc aaccagttga cacattactg atcctatatt
     2281 tatattatct caaggaggaa caaccatggc acaagaattt tcatatgaaa tcgtagaaga
     2341 aatcgctata ttgtcagaaa acaacaaggg gtggcgtaaa gaactgaacc tagtcagctg
     2401 gaatggtcgc ccgccaaaat tcgatcttcg agattgggcg ccagaccatg aaaaaatggg
     2461 gaaaggcctg acattgacga acgaagagtt tgaacaactg caaaaagcaa tagaaaatat
     2521 gtaatgagga aggaccctgt cgaatgaaaa gtatgactgg ttttggcaaa gccatccgcg
     2581 aaacaaaagc gtatcagtta gatattgaaa taaaaagtgt caaccagcgt tttctagatg
     2641 ttcaaatacg aagtcctaaa atcctgaact ttgttgaaaa cgattttcgt cagcttatta
     2701 aacagcagtt aagccgcggt cgaatcgaag tatttgtgaa tctagcttat attggcaaaa
     2761 acgaaaaaca gatcgtcatc aactgggaat tgattgattc acttatcact caattgacag
     2821 acggaattca acaacgatac ggtagtcaaa atcaagtgtc aattagtcgg atggcggaac
     2881 gcttcgcgct ggacgaaaac ttcgtaacgg tagaagaaaa accggaagag agcatggaag
     2941 aattggcaca gttagctcta gatacattga aaagcgcact tgattctatt gaggcgagtc
     3001 gagaaaaaga aggtcaagct ttagctttag tgttgaaaaa gaatactgaa gaattcaaag
     3061 aagtactaaa aaaactcgat agttttgtag aaatctatga aaaggaattt caaataaggt
     3121 atcaaaaaaa gctggaggaa tttcttggag cgaaagtcga tgagcaacgg ttgttgacgg
     3181 agctagccat cttgttggaa cgcggagata ttcacgagga actggatcgt ctggcgatcc
     3241 atgtacaaaa actagatgaa cttatcgaag cacaatgtcc tgtaggtcgg gaattagatt
     3301 tcctgatcca agaaatgaat cgggaaatca acacgatcgg ttccaaatca aatgcaatcg
     3361 aaattaaaaa tcaagtcgtc cagtctaaga cgatcttaga aaaaatcaga gaacaagtac
     3421 aaaatattga atgacgaaag aggtagagat aatgaaatat tatgacgtaa cgttccacga
     3481 attaagtgga aaaactgtgg ttaaacggga tatcccttct gaaaaaaacg gctttgacgt
     3541 atggaaagat gcctgtgcag actataatga gaacgaactg tttattttga tcaacgacgg
     3601 tgcttacgtg acgatgaatc ggaaatttat tgttcgaatt gacacagaag aggtagaaga
     3661 tccgacagaa aaagcacgtt ctcgaaaaga tgagatcatg ggtgtagtga atactttgtc
     3721 caatatgggc ttttagcaag gatgctgaac aaagaaatac aataaataat cagaattgga
     3781 tttcttcgct tagcttcttt gctctaggaa tacatttaaa ggttgtgaca aaattcgtgt
     3841 cacaatcttt ttttattttg aataagtgat gttcaaaaac cattttctcg gtatt
//
LOCUS       DAKBVG010000202         3894 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000202 DAKBVG010000000
VERSION     DAKBVG010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3894
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.202"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            53..1936
                     /locus_tag="OUD09_002364"
     CDS             53..1936
                     /locus_tag="OUD09_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCT9693596.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(2011..2295)
                     /locus_tag="OUD09_002365"
     CDS             complement(2011..2295)
                     /locus_tag="OUD09_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HCT9693597.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(2285..3019)
                     /locus_tag="OUD09_002366"
     CDS             complement(2285..3019)
                     /locus_tag="OUD09_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HCT9693598.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            3148..3771
                     /locus_tag="OUD09_002367"
     CDS             3148..3771
                     /locus_tag="OUD09_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HCT9693599.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
BASE COUNT         1166 a          705 c          845 g         1178 t
ORIGIN      
        1 taatacccgt atattacgaa tgaaaaaagt ttatgaaaag ttggaatatt gaatggaaaa
       61 acaaaaaagg ttaaaaaaca gccgctacat cactgggttt gatgggctcc gttcattagc
      121 tgtggtgggt gttatcttct atcaccttct gccaactagt atgaaaggcg gctatttggg
      181 cgttccgatt tttttcgtcg tatctggtta tttgatcacg gatctgctgc ggcaggaatg
      241 ggaacaaaat ggaaaaatca atatttggca attttatgtc agaagaatga agagactcta
      301 tccggcactt gtttttatgc ttgtgacagc ttcggcttat atcactttgt ttcagcgagg
      361 gctgttaaac aatcttcgag gagttgtggt cagcagctta ctgtatgtca acaactggtg
      421 gcaaatcaat aacggtttat cttatttcga tcgatttgct aatgagtcac cgtttacgca
      481 tatctggtct ttagcagtag aaggtcaaaa ctatttggtt tggccgatct tgtttgtatt
      541 gttgatggta tttgttcgta aaagaaaatg gatcttttat actgtgttag gtacatcctt
      601 cctctccgcc atcttgatga tgatcttgta tacaccagga ggcgatccaa cccgtgttta
      661 ttacggtacc gacactcgtc tgttttctat atggttggga agtgcgcttg cgtttgtttg
      721 gccaagtacg cgtttaaaga aaaacatccc gaaacaagcg aaaaatattc tgaatatcgc
      781 tggaggaatc tcgcttttgg cactgattct ttgcttcttc tttttagatg atcattacaa
      841 tttcatctat tacggaggaa tgctcctagt gagtctgtta tgcgtagtgc tagttgcagt
      901 gacagcacac cccggagcaa gtttgaatcg atggctgaca aatccagtat ttacttggat
      961 tggcaaacga agctatggca tttatctgta tcagtttcca gttatgattt tttatgaagc
     1021 aaaggtgact gatattgccg atcatgtttt gttgcatact ttagtggaaa tcacattgat
     1081 tctaatcatc agtgaacttt cttatcgata tgtggagcgt ccgctagcac gttttgatta
     1141 tggtcgtaca ttccaagtga tgaaaggatg gttcagcaaa ccgatcatca gtaagagcaa
     1201 accatgggtt gttccaggca cattacttgt agcaattgca ctcgttggtt tcgttacggc
     1261 accgaaaaac gctgtaacag ccgatcaaaa acaactgcag gcaaaaatcg ctgaaagtaa
     1321 aaaattagca gaagaaacca gaaacaattc agaaaacacc actagttcgg ttgaccaagc
     1381 gatccttgat aagtacgagt tgtccaaggc acaagggaat aaggcacaaa aattagaact
     1441 aacggcattt ggtgattcag ttatgttgga tgctgcaacg gatctacaag aactttatcc
     1501 aaaagtagtg gtagatggag atgtcggacg acaattatat gccagtgtac cgtacattga
     1561 aaaattaaag gaacaagatt tgttgaaaga cactgttctg atcggactag gaacgaatgg
     1621 ctcattctcg gaagctcagt ttgatagtgt gatggatgca atcggtgatc gaaaagtata
     1681 ctgggtcaat gtccgagtac cgacgaagcg ctggcaaaat gacgtgaatg cgatgcttga
     1741 acagatgacg aaaaaatacg acaatatgac gctgattgat tggtacagct acagtaatga
     1801 ccatagtgac tggttttatg atgatcaagt ccatccaaat cctgaaggga tgacacatta
     1861 cattcactta gtcagcgaag ccttgctagg aggaagttca agtacgaaca gcagtgagac
     1921 aaacgtgaca gaatgatagc tacaaaaaac ttcaggcaaa aacatttgtt tttgcctgaa
     1981 gtttttttat atttaccgtg tttttaacat tcatatagat tcaggaagca aaacggttga
     2041 gtgtgtctgt aaataacgtt ctttttgttt tcgggtgagt tttttgaatg cagcttctgc
     2101 ttttgttgct tcgcttcttg aagaaaatac ttctgcatgg atcatcttaa caggtcttct
     2161 tttttgaaga cgagtatatt tagcaccagt accattgtta tgttcctcta atcgtctaga
     2221 aagatcggtt gtatatccac cataaaatgt ctgatcttta cagaataata cgtaaaaata
     2281 atgttcattt gccatagagc atctcacgaa tctctgcagt gtattctcca tccatattgt
     2341 aagtgatcaa tggtggagcg actttgaatc cgtctaattt gccttctttg attccttcaa
     2401 ctaacaaaat atttgcctct tttccaggtt ttgggtaaac aaattggatc cttttaggtg
     2461 caattcgata gtcttccatg acctgaatga tttctaagaa ccgatcagga cgatggacca
     2521 tagtaaagcg tccatttgtt tttagtagct tacttgaaat atcgatcact tcttctaacg
     2581 tcgtatgaat ctcatgacgg gcgattgcca ggtgtttatt agggttcgtt ttatttgttg
     2641 gcagcccttt gaaataaggt ggattacaga cgactaagtc acaagaatta tgtcgaacga
     2701 cagataagct gtctttcaga tcgatggtgt ggacagtcat ttgttcattt aaatggttaa
     2761 gcgcgatact acgtcttgcc atgtctgcta gacgaggttg aagttcgata gcgtcgatag
     2821 ccgcctgggt tcgtttactt aaaaaaagtc caactgcgcc atttcctgca caaaggtcaa
     2881 caatcttccc ttttttaggt acacgggaaa agtgagcaag cagcacagca tctaaagaaa
     2941 aagaaaacac tttggaactt tggataatct tgatatcttc tgcgtataat tggtcgattc
     3001 gttcgtcttc gtgaagcatg gattccctcc aaaatgaaaa aaatagtcgt ctgtattata
     3061 ctatttttat taaaaagaac caaggtggct tgtcaaacct ctatttttta tgcatactat
     3121 gaccataaga agggaaagga agagactatg ttttatcgat ttatgcgcgg ggtagttcga
     3181 gtactattag ccttagttaa tggaaatgcc cgctatgaga ataaagaagt tttaccgcaa
     3241 gatgagaatt acatactagt tgctccccat cgtacttggt gggacccatt atatctggcg
     3301 gtagctgcag cacccaagga attcagcttt atggcaaaag aagaactgtt caaaaatcca
     3361 gttctgcgat ttatattaac tcgatccaat gcttttccag ttaaaagaga aaatccagga
     3421 ccaagcgtca ttaaaacacc tgtaaagatt ttgaaatcga caaatttgag tttgatcatg
     3481 tttcccagtg ggacaagaca tgctacagaa cttaaaggcg gaatggcatt gatttccaga
     3541 atgtctaaag cgaaaatcgt gccagctgta tatcaaggtc cgttgacact tggggatctg
     3601 ttcaagcgtc agagagtgac agtgcgtttc ggcgaaccaa tcgacttatc agatatcaag
     3661 aaaacggata aagaaggtat ggcggagatc gaacgacgaa cacaagcagc ttttgacaaa
     3721 ttggacaaag aagtgaaccc tgatttcaaa tatgaagtca aaaaaaaata aaatgataag
     3781 tttatgcgtg ggcggtcttt tgaatctact gtcctgttca gttcagactt tttcaatgaa
     3841 aacgagggag agacgtcaag tctgccctct ttttgcgttc caatatgtaa aata
//
LOCUS       DAKBVG010000203         3880 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000203 DAKBVG010000000
VERSION     DAKBVG010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3880
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.203"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            15..680
                     /locus_tag="OUD09_002368"
     CDS             15..680
                     /locus_tag="OUD09_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693600.1"
                     /translation="MAYYLLSEPKQIAASNIFTAVMTLMCVMCFSIFSYLLVQFIRQN
                     LTMSKLFKSLLDLLILGSMLTTLTDRFEQSEKTVEGIKNFLTTIDQFINGYMYLFEVG
                     FILTLFGCVFIKLIKRSIEKEMVIDLIPTLSGYEYYVDFGKYEELIHTDIVHTSIHEV
                     TVKGIRSTLKDGQETKTIDRRKEVYNFYKVEASLRYGIGKLRLDGSNIQQNKPYVLGK
                     VVI"
     gene            724..993
                     /locus_tag="OUD09_002369"
     CDS             724..993
                     /locus_tag="OUD09_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693601.1"
                     /translation="MILLYRIVLIVGTELKILKKEQLFKVKEHHNQLLCIKTGTMNIQ
                     YRTEYLELTNQTDFLSIREQHKQEKLMATYSQLIDEWEQFKESKT"
     gene            1225..1521
                     /locus_tag="OUD09_002370"
     CDS             1225..1521
                     /locus_tag="OUD09_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693602.1"
                     /translation="MNVKIRDLDPKFISEIDRRCVELSNRTGNKWSRNDYLKLLVEND
                     FDRPLMEYKQEKFDQLLEKFSEIQSYNTKILEEYVSQNNRIIEILIEQNRGSEL"
     gene            1523..1945
                     /locus_tag="OUD09_002371"
     CDS             1523..1945
                     /locus_tag="OUD09_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693603.1"
                     /translation="MAKVIDKHIYFHQTHIDVLDETISTHAEVKNYSEAVRFLCMNYD
                     PEVEKKVNSNQAKLNGMSKEISILTEMVSEFADLHLKDTGILIGKNSTVYQEAKERVE
                     GKIKSNQTKKYSPQLKKPVNESVHARVDPFDPTKILKR"
     gene            1964..3556
                     /locus_tag="OUD09_002372"
     CDS             1964..3556
                     /locus_tag="OUD09_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693604.1"
                     /translation="MTQSPSVILTCQFTLPNKKSFSTYIDYITREQALEEKERRTPEE
                     EQELQRIKNALEEFYIPEGETFSESKTGKEFSETSNEAQSLMEHGINFDKLEEQDFTN
                     YLSYMTRHYALEQKKELTQSEQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSIASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPDKRSEWNYGDEKRTKLSPYTREKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVD
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            3577..>3880
                     /locus_tag="OUD09_002373"
     CDS             3577..>3880
                     /locus_tag="OUD09_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693605.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVNGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKS"
BASE COUNT         1517 a          555 c          767 g         1041 t
ORIGIN      
        1 tggtggtcta tggggtggcc tattacttgt taagtgagcc aaaacagatc gcggcgtcaa
       61 atattttcac ggcagtaatg acattaatgt gtgtcatgtg cttttctatt tttagttact
      121 tactcgtcca atttatcagg caaaatttaa caatgagtaa acttttcaaa agtttattgg
      181 acttacttat cttaggcagt atgttaacga ctctaacaga tcggttcgaa caatctgaaa
      241 aaacggtaga ggggataaag aacttcttaa cgacgattga tcaatttata aatggatata
      301 tgtatttgtt tgaagtaggc tttattctca ctttgtttgg ttgtgtattt ataaaactca
      361 ttaaaagatc aatcgaaaaa gaaatggtta ttgatctaat tccgacgctg agcggctatg
      421 agtattacgt tgattttgga aagtatgagg agttgatcca tacggatatc gttcacacga
      481 gtatccatga agtaaccgtt aagggcatca gatctacttt aaaagatgga caggagacga
      541 aaacaatcga ccgaagaaaa gaggtctaca atttttataa agttgaagca agtctacgat
      601 atggcattgg gaaattgagg ttagatggga gtaatattca acaaaacaaa ccatatgttt
      661 taggaaaagt ggtgatttag aacttcctat gaaagatgaa taaataagga gaatcttttg
      721 aaaatgattc ttctttacag aattgtgctt atagttggca cggagttaaa aatcttaaag
      781 aaggagcagt tattcaaagt gaaagaacat cataatcagt tactatgtat taaaacgggt
      841 acgatgaaca ttcaataccg aacagaatat ttagaattaa cgaatcaaac tgattttttg
      901 tcgattcgag agcaacataa gcaagaaaaa ttaatggcta cttatagcca attgattgat
      961 gagtgggaac aattcaagga atctaaaaca taaaattcca tttaaacacc ttatttttta
     1021 aagagcataa ggaaacgtga ccccactttt aaaaaaagtg gttggatagg ctgtgcctat
     1081 cacgtttctg aaaaaaataa atgaccccaa aataccaata tataggatag tacaaggata
     1141 gcaaaggata gtaaaaggat agaggtagga tagcgaaaag gataggatcc ttttcactat
     1201 cctatattat attggaggat atttatgaat gttaaaatta gagatttaga tccaaaattc
     1261 atttctgaaa ttgatcgaag atgcgttgaa ctaagtaatc gtacaggaaa taaatggagt
     1321 cgtaatgatt atttaaagct actcgttgaa aatgattttg atcgtccttt gatggaatac
     1381 aaacaagaaa agtttgacca gttattagaa aagttttcag aaattcaaag ttataacaca
     1441 aagatattag aagaatatgt tagccaaaat aatcgtatta tagaaatctt gattgagcaa
     1501 aatagaggaa gtgaactttg atatggcaaa ggttatagac aaacacattt actttcacca
     1561 aacacatatc gatgtattgg atgaaactat ttctactcat gcagaggtta aaaactatag
     1621 tgaagctgtt cgctttttat gtatgaacta tgatccagaa gtggagaaaa aagtaaattc
     1681 caatcaagca aagttgaatg gaatgagtaa agaaatttct attttaaccg aaatggtttc
     1741 agaatttgcg gatcttcatt tgaaagatac aggaatatta attggtaaaa attcaactgt
     1801 ttatcaagaa gcaaaagaac gagtggaagg aaaaattaaa tccaatcaaa caaaaaaata
     1861 ttcccctcag ttgaagaaac ctgtgaatga atctgttcat gccagagtag atccatttga
     1921 tccaacgaag attctaaaaa gataaaaatg aaggaggata ataatgactc aatcgccttc
     1981 agttattctc acttgtcaat ttactttgcc gaataaaaag tcattttcta cttatattga
     2041 ctatattaca agggaacagg ctcttgaaga aaaggaacga cgtacgcccg aagaagaaca
     2101 ggaacttcaa cgaatcaaga atgctttaga ggagttttat attcctgaag gagaaacttt
     2161 ttcggagagt aagacgggta aagaattcag tgaaacgtcg aatgaagctc aatcgttgat
     2221 ggaacatgga atcaattttg acaagttaga ggaacaagat tttaccaatt atctttctta
     2281 tatgacacgt cactatgcgt tagaacaaaa gaaagaactg acgcaaagtg aacaaaaaga
     2341 ataccaacgc ttgaaccaag caatgaaaaa atatcaaaca gaaccccata aaaaagcttc
     2401 aggaaatctt cctggagttt tttctatagc ttcagacgaa gtgaaaggaa gtgatctgaa
     2461 ggagattaag caaatcttcc aaagaggaca acaaaaaggc tcaatcattt atcaagatgt
     2521 tgtttctcat gataattcgt atcttgaaaa aataggattg tacgatccca aaacagatac
     2581 tttagatgaa gaagggttaa aagcagctgg aagaaaaatg atggagacat tattcgagaa
     2641 agaacaattg aatgaaacag gttactggat ggcaacgatt caccgtaaca ctaaacatat
     2701 ccatattcat tttgcagttg ttgaaaaaga aaatacgcgt aaaccttaca cggaggttga
     2761 aaatggtgtg agttatatcg taccgaaagg taagagaaga caagcaacat tagacgcaat
     2821 gaagaccaat tatgtacatg agttagaacg tttttcttat gaaaaggatc gaatgattgg
     2881 tatggagaaa cgaaatttat tgacgcggaa aagtgatttg cgtaacacct taacaaaagt
     2941 ggtaaaagag ccagcgcgtt acgatcgaca cgcgatgaac ttattaaatg aagtatataa
     3001 aaccctacct gacaaacgtt cggaatggaa ttacggggat gaaaaacgaa ccaaactttc
     3061 accatacaca agagagaaac tcgatgagtt aaccaaatat gttttaacaa aagaacctgg
     3121 atatcaagaa tatatacaga tttcaaaagc attgaaagaa gaagcaaaaa gtctttatgg
     3181 ggaaagcaag agagaatcaa aggatgcaga gaaaaatgct ctctttgatt taaaaaagcg
     3241 cacaggaaat gccatcctaa cagagctgaa gagacaggat agtcaaataa aaccattaat
     3301 agaaagtgtt gatcagatgc aatctattta caatgaacga tcctttcgaa cgcttaaaca
     3361 attgccaacg aatgaataca aatatagtta cgaagaattc atgaagcgca ggaaacaatt
     3421 ccaacaaaga gttgtcacgc agcggagttt gcgtcaatta aatcgaacga ttaatgccca
     3481 tgaagaaaaa taccacgcaa gtaaagcata cgaagagcaa caacgaagaa ttcaaatgga
     3541 acaagaaaga cagtaaagac agtaggagga atagcaatga aacaatattt agatcaatgg
     3601 aaagttatag aaggatcatt aagagaagaa cgaatcgaac aacttcctga ttgtttagaa
     3661 aaagagcatt tgttccagat aagagaaatg ttgagaaacg aacagtttga tccaaatcaa
     3721 tttttagtcg ttgagtaccc agctactggt gtgtattgtt gtaatcatgt gaatggtgaa
     3781 aaatatttta tcattcaaga atatgagggc aagttagcac cttattatac tacttgggaa
     3841 atgaatgaag agggaatcaa taactttcca tgtaaaagca
//
LOCUS       DAKBVG010000204         3851 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000204 DAKBVG010000000
VERSION     DAKBVG010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.204"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(163..2160)
                     /gene="tkt"
                     /locus_tag="OUD09_002374"
     CDS             complement(163..2160)
                     /gene="tkt"
                     /locus_tag="OUD09_002374"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCT9693606.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2262..2513)
                     /locus_tag="OUD09_002375"
     CDS             complement(2262..2513)
                     /locus_tag="OUD09_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HCT9693607.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2660..3283
                     /gene="lexA"
                     /locus_tag="OUD09_002376"
     CDS             2660..3283
                     /gene="lexA"
                     /locus_tag="OUD09_002376"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HCT9693608.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3463..3681
                     /locus_tag="OUD09_002377"
     CDS             3463..3681
                     /locus_tag="OUD09_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693609.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT         1064 a          818 c          715 g         1254 t
ORIGIN      
        1 tctcaagaaa cacaaactaa tccgaagaat tctttgtttt gtcatttaaa tgtaacatag
       61 ccgaaaacga aaagtaatat tgatttattc ttctaacaca taaagaacag ctgtctccaa
      121 cttgcagaca gctgttctta tcagtcaaac aattgcttgg atctatgata gttgattgta
      181 cgtattcacg acattttcta ctgtaaatcc aaattcttct aaaattttag tgccaggggc
      241 agatgcaccg aaatgatcga ttgtaatcgt tgcgccctct gttcctacgt agcgttccca
      301 tccaaatgat gcagcagctt cgatagccac acgtttcttc actgatttag gaagtacact
      361 ttctttatat tcagaagatt gtttttcaaa gaggtcaaag cttggcatag aaacaacaga
      421 aacatctttg cctttttcag ccaattcttt ttgagctttc acagccaaat caacttcaga
      481 acctgttgca atcaaaatac cttctggtgt ttctccttgt gatggagaaa gaacatacgc
      541 gccttttttc accatttcat ctgctacttc ttttgtagaa ggcaatactg gaaggttttg
      601 acgactcaat acaaggatag taggcgcatc tgttgtctcc atcgctactt tccaagcagc
      661 acgtgtttca tttccgtctg ccggacgaat cacttgaaca ccaggcatgc agcgaacaga
      721 tgctagttgt tcaatcggtt cgtgcgttgg tccgtcttct ccgacagcaa ccgagtcatg
      781 tgttaagacg taaacaacag gagtatgttg gattgctgct aaacgaactg ctggtcttag
      841 ataatcgaca aagacaaaga atgttccacc gtagatacga gttccgccat gaagttggat
      901 accattcata gccgaagcca ttgcaaattc gcggacaccg aaccagatgt tgcgtccttc
      961 ataatgttca ggagtgaaat ctttatctgc agccaccatt gtattattgg aacctgacaa
     1021 gtccgctgaa ccaccccaga agcttggtat agctttcgat aattcttgga tgacttcttt
     1081 acttgaaaca cggcttgctt gactgcttcc aacttcataa gttggcaatt ctgcatccca
     1141 gttttctggt agtttgccat caaaagcatc ttcaaattgt tgtgctaatt caggataagc
     1201 ttttttataa ttggcaaaca tttcattcca tgcatcttca gctttttgtc cttcttctat
     1261 catcgtttga tggaagcggg cagctacttc ttctggaaca gtgaaatctg gatactccca
     1321 accataaact tctttagcca tcttgatgcc atcttcacca agaggagcac catgaacagc
     1381 agatgttcct tctttaggag caccatagcc gatgactgtt tttacttcga tcaatgttgg
     1441 tttgtctgtt tcagcttttg cttcttcaat cgctttagag attgcttcta ggtcatttcc
     1501 gtctttcact aagatatgtt gccagccata tgcttcatag cgagcaccaa cgttttctgt
     1561 gaaagctttt gaagttggac cgtctagtga aatatcgttt gaatcgtata aaacgatcaa
     1621 tttccctagt ttcatatgac ctgccatcga acttgcttct tgagataccc cttccatcaa
     1681 gtctccgtca ccgcatattg catacgtata gtgatccatc acattaaagt tttctttgtt
     1741 atacgttgct gcaaggtgcg cttctgccat tgccataccg acagccattg caatcccttg
     1801 tcctaatggc cctgtagtgg cttctacacc atctgtatga tgtacttcag gatgtcctgg
     1861 tgttttagaa tcccattgac ggaattgttt caaatcatcg attgtcactt gatagccagc
     1921 taaatgaagc aagctataca acattgctga cccatgtcct gccgaaagaa cgaaacggtc
     1981 gcggtcagcc cagttttttg atgtcgttgg atttactttc aggtgttttg tccataacgc
     2041 atatgccata ggtgctgcgc ccatcggcaa tccaggatgt cctgagtttg ctttttggac
     2101 tgcttcaata cttaatgtac gaattgtgtt aacgccaagt tggtcaattt tgtcaaacaa
     2161 aataacccct cctattaatt taggtttttt ttacctttat ttcttacact ttcattctat
     2221 aagatgtcac acgaaaaagc aatctttttc agtatttttt actaaaagtt gtcatttcgg
     2281 ttgtgtaagc cttttttctt ttggatttct tttaattttt ccggggtcac gtcattgccc
     2341 tcaggatcga cgactttcat tccttcgata tgatgacgca taccggaacg aaaagcttcc
     2401 aaatactctt tgcgcaagtt gatttgttct tgttcttcgg cttttgagag accattttcc
     2461 ttctttttac gtgcaagttc attgatccgc tctatttttt caggtgacag catgctcagc
     2521 cctcctttta ctctccttca tattatataa aaactgaaaa aaaaacaact agataaacta
     2581 aataaaatac gaacattcgt ttgatttagc gctttttata tggtactatt tatttatcaa
     2641 gtgaaagaag gtgtaccttg tggcccgaca aactgaatca agacaaatcg aagtcttacg
     2701 atatatccat gaacaagtaa cccaaaaagg atacccgccg actgtccgtg aaatcgggga
     2761 agctgttcag ctttcttcta cttctactgt tcacggtcat cttgcacggc ttgaaaaaaa
     2821 agggtttatc caaagagatc cgacgaaacc tcgtgcaatc gagttgacaa aacaaggttt
     2881 agaaaagatt gggattcgtc caacaactat tccagtactt ggcgtcgtaa cagctggaga
     2941 acccatttta gcagtagagg atgcttcaga cttctttcca atccctccta atctttcttc
     3001 cgaagaaggc agtctcttta tgctgacaat tcgtggagat agtatgatca atgctgggat
     3061 attggatggt gacaacgtga tcgttcggaa gcaagaaact gctcaaaatg gcgatattgt
     3121 cattgcaatg actgccgagg acgaagcgac ttgcaaacga ttctataaag aaaaaaactt
     3181 tttccgtctg cagcctgaaa acgacttgct cgaaccaatc atcttagatc aagtatctat
     3241 attaggacga gtagtaggac tttatcgtag tcatatctat taattgctac taattaaaag
     3301 aaaacttttt aaaaagagtt tgagacgttg gtctcaaact cttttttcgc ccgggttctt
     3361 cttctttcaa atttttatca cttccctctt tacaaaccga acaaatattc gtatactaaa
     3421 tatacgaaca attgttcgca taaattaaag aggtgagaat tcatgaaatc aatgagacgt
     3481 cttggcttag tattaactgt tttgttttta ggtattatta ttggtaattt cggcatacgt
     3541 ccagctttac ttgtgtttgt tccgttattt actttgtggt tcatgttgtg ggatgaaaag
     3601 aagtaccgtc aatctgagcg gcgcagaatt catgaagatc atttctatca tgagccctac
     3661 tacagatcca ggcaatcata ataaggattg actgctaatt attcaaaaga caagaagtta
     3721 aacttttacg ggggtgtgac aaaaatcggt cacatccttt ttttcgaata aacggtgttc
     3781 taaagccagt ttttcggtat taagtcaaat tgcggaaaaa gtgatttttt aaacttctac
     3841 tggtctttgc t
//
LOCUS       DAKBVG010000205         3836 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000205 DAKBVG010000000
VERSION     DAKBVG010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3836
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.205"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            63..383
                     /locus_tag="OUD09_002378"
     CDS             63..383
                     /locus_tag="OUD09_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCT9693610.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            401..832
                     /locus_tag="OUD09_002379"
     CDS             401..832
                     /locus_tag="OUD09_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HCT9693611.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            962..1423
                     /locus_tag="OUD09_002380"
     CDS             962..1423
                     /locus_tag="OUD09_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HCT9693612.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            1457..2092
                     /locus_tag="OUD09_002381"
     CDS             1457..2092
                     /locus_tag="OUD09_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HCT9693613.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQIEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            2205..2840
                     /locus_tag="OUD09_002382"
     CDS             2205..2840
                     /locus_tag="OUD09_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693614.1"
                     /translation="MDNRNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            3139..>3836
                     /locus_tag="OUD09_002383"
     CDS             3139..>3836
                     /locus_tag="OUD09_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCT9693615.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNY"
BASE COUNT         1505 a          551 c          655 g         1125 t
ORIGIN      
        1 aaattatttc tgcgtatttt tctaaacttt ttttgtaaac atttagaaag gttgttttat
       61 cgatgaatac ctatgaaata ataaaagact tggcgaagag gaaaaagata tctatacggc
      121 agttagaaat taattttgga tattctaacg gttatctagc aaagtggaaa aataatactc
      181 ctaattcaga tgaattaagt cggattgctg actatttcga tgtctctgta gactaccttc
      241 taggtagaga agaaagagaa acccctaaac atgtggattt atcagaagac gatactgttt
      301 tttcttttga tggaaaagaa atatctaagg agacaatgcg taaagcgatt gcaattgcta
      361 aagctttaga ggaaaatgaa tagttggagt gatggtttgt atgtatttga aattgaaaga
      421 aatgttgagc gaatacaatt tgaaagtagt ttatatggag atgaaagagc ctggtttcta
      481 ctatccaaaa cccagaatca tttttttgaa tgaaaatcta tacggtgaaa ctgcagaagc
      541 ctttcattta tctcatgaac ttgcacattt cagcgcctct cattttgaat tttcagtttt
      601 gtacgatact tctacaactt ttcactcaaa atttgaaaca gaagcagata aaatagctat
      661 tctaatttta ttaaatatct atattgagaa cgaactgact gatgaatctc agtttaatct
      721 cgaaaaattt atggagtatt attctattca gaataagctc agatacactt gttatgctgt
      781 ctgccaatgc tattttaaga aaaaatattc ttacgcaagg caatatgtat gaatacatca
      841 agaattatta aaaaaaccga tttcagcaaa acattaaaaa agcccgtgct gcaacacggg
      901 ctctttcctc atatatgagc ttctacaaaa aaaatcatat cacagaaatg aggaagaaaa
      961 aatggaaaaa gagattttaa taaaatctcc aggaaaaacc ttgattaaag taactgatga
     1021 ttctatttca attattagaa aaggatttat aaatttagtt aatcaaggta ttaaaggaga
     1081 aaaaactatc ccctttaaaa atatatcagc cgtacaatta aaaaaaccag gtatgagtaa
     1141 tggttatata caatttacct tgctaggcgg aaacgaaagt cgcggaggta tattagcggc
     1201 cacgaaagat gaaaacaccg ttatgtttac aaaaaaatat tggaatgaaa tggaaaattt
     1261 aaaaaaatat atagagaagc aacaatctat attagataat aattccaaag aaacacaaat
     1321 ttcaagtgca gatgaaatta aaaagtataa agagttgtta gatgaaggga ttattaatca
     1381 agaagaattc gattttaaga aaaaagaatt gcttggttta taatattcaa tatcaaaaga
     1441 gaaagaggat ctattaatgg acggtatatt tggttttact ggtattgttt tatttattat
     1501 aggtttaatc atgttaattg taagatttat caaaaaaaca aataagaaaa cacctatgat
     1561 tctccttatt atcgggatta tttttactac cgtaggtttc tcgctttcct catcaaacga
     1621 ggcaaatgac aataacaaac aggaaagctc atcatctgat actcaaacta ctacatctaa
     1681 cagcagcaat aaaagcctaa ctcaaattga agaattttta gagacaaata atcatgactg
     1741 gggtgctttc ttagatagtt attattctat tactccagct actgagcaaa atacagcttt
     1801 tactaaatat attagtggta aaacatatac ttttgaagga actgttatcg aatcaatgac
     1861 tactagaata gccattatag cagacaaaga gtacgatgat aaaagttgga atgatatttc
     1921 tgcaacacct aaagtttcat atgtaatttt tgcgaaggac ttaaacaatg ctgatacctt
     1981 tacaaagggc gataaagtaa cttttacagg agaaattagt tcaagaggat cgaacataga
     2041 aaaatcctat gctcaatggg atatgattaa tagtaaagta agtaaaaaat aaatgaacca
     2101 cgccccacta agagttagat tgtgcacaat aataaaacct atacaaaaga gtgccatcag
     2161 gagtcacaat gcttgtaaag gaaatgacaa aataggaggt aactatggat aatagaaatg
     2221 aaaaactaac caaaaaagaa tatcttgaat taatagttga aggcggtatt ggagcaattc
     2281 cttatattgg tggcgttgtg cagtcacttt attttgggtc taaaaacgag aagcgtttca
     2341 aacgcatcga gaaattctac aaaaatttaa acgaatcatt agaaaacatt cgcgatcaaa
     2401 ttccagaaga tgttttcaac ttagagaaca aggaccaact cattggaata ttcgaaggga
     2461 taaatgacga ggtagaaaaa tcaaaggcac aaaataaaat agatatgtat aaaaatcttt
     2521 ataagaattg tttacttcga attaacaacg cttcatggga taacgaggag tattatcttc
     2581 ttgtactgaa tcaattaaca tcgattgaga ttcaattgtt agcatgctta atgagtcgtg
     2641 gaaacgaaaa tttcactgga aatatcagca tgtctggcta ttcacaagag ctaatagatg
     2701 gtagcttaaa tagattatct gattttggtc tgttagaaaa aacgattagc tccatagtat
     2761 taggtgatgt cggcaaacaa aatatgggct acaaaatatc tcatttagga attcaattta
     2821 ttaactcaac tcttacttaa gttttctttc taatatgaca tctagaccta tcagcaatgc
     2881 aacaataaaa aaccagacat accaataatc aacaaataat ctaaactctg gaagttcagt
     2941 tattctttta aaaatcactt taatcataat aaacacctct gattggatga tctaatacac
     3001 atccaattat accattaaaa cgacaaatag cacatccttg cgccggcaag caaaaaggat
     3061 gtgcaaaaaa taataaaacc caaacaatgg gcttctttat agttcctatt gtatcagaga
     3121 aagagagctg attcaattat ggcaagatta gtcaaacgtg gaaatagttg gcaatacgaa
     3181 atttcataca aaaaagatga cggaaaatac acgaagataa gaaaatcagg atttaagaca
     3241 aaaggagaag caaaagatgc cgccaacgaa ttagaatata acttgaacaa aggccttaaa
     3301 ggggatcgca aaaatctatt attatcagat tactttgagg attggatgca actttataaa
     3361 gaaggaacag tatctcctat cacttataga aaatacgaag atacgttaat gaacataaag
     3421 aaatatatgc cagcggtatt gatttctgat ttagatagag tgggatatca acgcttttta
     3481 aataagtatg ctaaagacca tgtaaaatct accgttatta agtttaataa ccatattaga
     3541 gcgtcgttga aagatgccgt agaagaagga ttaattccgt ttgatccaac tagaaaagca
     3601 gtaatcaaag gaaaagattc attgaagcca aaagaagata aatatttaga ttatgatcaa
     3661 tttaaatctt taatgaaact cgtagaagaa aacctttctg cgcagtactc ttctcctatg
     3721 ctcgtgttag ttgctggtgc cactggaatg cgatttgctg aacttctagg attaacatgg
     3781 gaagatatcg atttcgaaga tcaaatcatc actattaata aaacatggaa ttataa
//
LOCUS       DAKBVG010000206         3785 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000206 DAKBVG010000000
VERSION     DAKBVG010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3785
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.206"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            <1..171
                     /locus_tag="OUD09_002384"
     CDS             <1..171
                     /locus_tag="OUD09_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCT9693616.1"
                     /translation="SLFPLYHGSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFK
                     IEYTKKRQRLAY"
     gene            406..642
                     /locus_tag="OUD09_002385"
     CDS             406..642
                     /locus_tag="OUD09_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693617.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            649..2601
                     /locus_tag="OUD09_002386"
     CDS             649..2601
                     /locus_tag="OUD09_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HCT9693618.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2673..3170)
                     /gene="dfrG"
                     /locus_tag="OUD09_002387"
     CDS             complement(2673..3170)
                     /gene="dfrG"
                     /locus_tag="OUD09_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HCT9693619.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            3400..>3785
                     /locus_tag="OUD09_002388"
     CDS             3400..>3785
                     /locus_tag="OUD09_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCT9693620.1"
                     /translation="MKYILSFCTPKLNYLLAYIRLYSGVLHLRDSVRVSEKEKIKVTE
                     MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRKKIENPHPLLQTTVE
                     PSKPEQREMLLDALLEISDSDPLLRY"
BASE COUNT         1344 a          614 c          655 g         1172 t
ORIGIN      
        1 tctctgttcc ctctttatca tggaagtgca aaaagtaata tagggattga taaccttata
       61 gaagttatta ctaataaatt ttattcatca acacatcgag gtccgtctga actttgcgga
      121 aatgttttca aaattgaata tacaaaaaaa agacaacgtc ttgcatatta agtagttcag
      181 ttttggagta caaaaagtga aaatgtaaac ctaaatgagt atcaaattgc aactgaattt
      241 gatactcgtt ttttattgtt aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag
      301 ttaaaatgta tataaaaatg cgaacgtgaa ataattattg taggaatgca aattggtgtg
      361 ttaaaatggt tacaatttac taagcaaaaa ggggtgcatt ttcctatgca atttcttgag
      421 ccaaaaaata agaacgctaa gtctgtggat tgggaaatat cagagcaagt aagggtgata
      481 gttaagcaat acgctgaata tgcagagcgt actgaaagcg aagcagtgga tgaattctta
      541 ttgaacattt tagacgataa gaagtttatt gaatggattg ccaacaagcg aagtaataag
      601 cggattgttg aaaaaatggg tatcaaagat agagtgggtt gatagacaat gaccaagctt
      661 aaaagattag ccacgaagga agatgaaatt gtagatgtta aaattccgat ttcaaacgaa
      721 gaattaaaag atagggctaa acaatatgat ttattaactc caaaacgatt tgcaacaagg
      781 tataacaaaa tgttgttctt gccaacgtct tttaaatgga acggttctga atatcccatt
      841 caatataatt actgcatcaa tccgttttgt tgtaattttg ggaaagaaca gcacaaattc
      901 aaagatgtaa aagggaaacc aagtcgatat aaaatgactg gtagttccaa agataaaggt
      961 cataaaggta tgtactgcaa tgacaatcct attggcagag gtgtatcgca aaactgtaca
     1021 gtaacccctt tatctaattg gtctgttgtt gaagaaatta aacgccttat agaaatcaat
     1081 agcatccaag atgttgagcc agattatcaa tttcataaag aaggctgctc ggaagaagaa
     1141 tcaaccccat tcaatgagcc gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct
     1201 caacgctatc aatgtaaggc gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa
     1261 accactactt atcaccaaca aaagaacacc attcttccaa tgttcgctaa gatggttgtt
     1321 ggaagggttt cagtcagtcg tacttgtgat attttaggaa ttggagttgg aacctactat
     1381 cataagctgg agtggctgta taggcgttgt ttggagtttt tagaacgata tgagacacaa
     1441 ccccttcaaa caaagaaatt taacgagatg tggcttaata cggacaagat gcactactac
     1501 ctaaataacg tccgtaagaa aggacaaggt tcaaagaaat atacggggtt tgaagacctt
     1561 aatatgcaaa cgtatatagt tgtatctgct gaggtcttat caagatatgt gttccgttct
     1621 gatgttgcct atgattggaa tatttcaatg gatgaattaa atgaagatac aagaaaattc
     1681 aaagaagacc atctcaatac ttttagtcgt aaaaatgata ggttagattg gtcttattat
     1741 ccacaagaac catccgctaa cgatagcgaa aataggaatg cttatcttca cgaattaggg
     1801 aaaattacta ataggagtag atttgtcgat ggtttaaatg ttgatgctcc ttatactaca
     1861 actgctcact attggttaat taaacaaatg gttaatgccg acgaatggcg aatgattagt
     1921 gatgatgact tctctattag aaacgccttt tatagagtat tcacgaaaga attaaggttg
     1981 agcgatgcac atcattttat ttgccaagtt aacaagacga aaagcagaaa acaatgctta
     2041 aaagaattcg gacaagcaaa agcagaactg ctcgattggg gagatatcag aggattcaaa
     2101 acaaagtttc tacggacttt ggcatctcat tatctaactg aattactaac aagtcatcaa
     2161 ttccacgaag aggctattag taaagatgga gaaagatatc gcaaatatgc cgataaccct
     2221 atcaaacatc cattagccac taaggataaa ggattttatt cagttgattg tagaacggat
     2281 ttatctgctc tggaaccaaa cgaaatcgca aagatgctat taaatgttaa tgaccattcg
     2341 accaatagct ttattcaaca gataagacgt tatatatcat ctcttgaaag accattaaca
     2401 acagctcgtg gtgataagaa gagctatatt tacgctaact tcaatccgaa gtatgctcaa
     2461 tttgcgatta caattctaag aacttactac aacttttgtc gcccttttaa atcagcggat
     2521 aaaaaggttt tgacacctgc tcaaagatta gggattacag ataaacaatt tgattggaaa
     2581 gatataattt atttcaaatg acaaaaagag cttggagtaa tcccaagctc ttgtgcttta
     2641 tctttactgt tcttcttcaa ttaacgcacc cgttaactca ataagttttt tctttcatat
     2701 acatgaaaat agtagttata cggattttta tcattcttta tccctttttg ggcaaatacc
     2761 tcattccatt cctcataatt cacttctgga aaaaaagtat ctccttcgaa ttcatgatgt
     2821 atttttgtga tgtacatttt ctcaacataa gggaaaaaca aattataaat ctgttctcct
     2881 ccgaaaataa aaatttcttc ttcgttttta cataactcaa aaacatcttc tattgaatga
     2941 acaatttcac aaccattaaa ggtaaacccc ttatctctcg tcagaataat atttcttctg
     3001 tcaggtaagg ctcttccgat tgattcaagg ttcttcctac ctaatattat cggatgtccc
     3061 tttgtagtat ttttaacata ttcccagtcc ttgggaatcc tccaaggaat gtcattctct
     3121 ttgccaatca ctctattctt atccatcgca gcaatcaaag aaactttcat ttataacaca
     3181 gcctttttaa ctaatttacc atctaatttc ttatttctat caaaaaacct tttctcatta
     3241 tcctgcccgt tagttgaata agcaattttt tctataagtt atattatcat aataccacaa
     3301 aatcacaaat tttcattcaa ttatcatttt caattgcatt ttataaatca ttttgtatgt
     3361 aatttggtat gtaagttaaa atgaaaaaag tagtccattt tgaagtacat tttgtcattt
     3421 tgtactccaa aactgaacta cttacttgca tatatacgcc tttatagtgg agtactacat
     3481 ttacgagatt cggttagagt atcagaaaaa gaaaaaataa aagttacaga aatgtatact
     3541 tcaataaatg gtgaattatg taagattgat agagcttatt ctggagaaat tgttattttg
     3601 caaaatgagt ttttgaagtt aaatagtgtt cttggagata caaaactatt gccacagaga
     3661 aaaaagattg aaaatccgca ccctctacta caaacaactg ttgaaccgag taaacctgaa
     3721 cagagagaaa tgttgcttga tgcccttttg gaaatctcag atagtgatcc gcttctacga
     3781 tatta
//
LOCUS       DAKBVG010000207         3736 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000207 DAKBVG010000000
VERSION     DAKBVG010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.207"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(296..1024)
                     /locus_tag="OUD09_002389"
     CDS             complement(296..1024)
                     /locus_tag="OUD09_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HCT9693621.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1002..2576)
                     /locus_tag="OUD09_002390"
     CDS             complement(1002..2576)
                     /locus_tag="OUD09_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HCT9693622.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2805..>3736
                     /locus_tag="OUD09_002391"
     CDS             2805..>3736
                     /locus_tag="OUD09_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCT9693623.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSAL"
BASE COUNT         1150 a          551 c          691 g         1344 t
ORIGIN      
        1 atttaaataa ctacatccat ttattctatc ttctaaaata gcctttttca tttcagttaa
       61 atagttcagc gccatttttg cactcgactt gcatagtagt tttttccata ttcctcaacc
      121 tcagcggggg ttgaagacat aaggaaaatc ctatgcgtta tgctcaactg gatctgatta
      181 aatattctaa tgtatttttc tctaccatca ttaaatggtt aatagccaaa aatcaccact
      241 tttcaatggc tccttgttgt ataagtataa ctagaagatg aacacgtctg agaaattata
      301 gaagcttttt acggtaatta ctggggctgt gtccagtagt ttttttgaag agaacagaaa
      361 aatagctcac gtgatctatt cctacttttt gactaatttc atcaatagat aagaatgtgt
      421 ctttcagtag agcacttgct cttttgattc gaaagtctag caagtaatca tgaaaggaat
      481 aacctgtttc ttttttgaag gcccgtccta aataagcggg atggtaatgg aattgtgtag
      541 ctacatcttt gagcgtaagc ttttcactat aattgttttg aacgtaatca atcacttgtt
      601 gaattgactc attaaaagaa tcatcagtta gagagttttg attagctgct gatttacgca
      661 ttttttcttc ttgtactaac ttaactaaat aaggaaaatc aatgggtttc gttatgtaac
      721 ttttaataga taaatcaatc cccttttttg catactgaaa attttggtgg gcactaatga
      781 caatactagt aaagtagcga ttggatgttt gttgcaaaga ttcgattaat tcgagtccag
      841 aaattttccc catcgtaata tctgtgataa gtaaatcaaa gcgctggttt tgcaggtttt
      901 taaatgcttc gtctccgtct gtgtagcagt gatcgataat gatgtcgggg actttcttca
      961 aaagataatg ccttatgcct tctacaattg tacaatcatc gtcaacaatt gcaattctca
     1021 acatgcatat tccccccttt ttgtattttg atagtaatcg ttgttccgac gtgtagttgg
     1081 ctttctataa caaaacgtgt atctccagga tagagatgct ttatacgtcg aaaagaattt
     1141 gagatgccga tattctcact ataaggaata tcatcattaa gtttttgttg aatatgctcc
     1201 agcttgtctt ggtccattcc tttcccattt tcaacgattt ttaacacata atggttggtt
     1261 gtttcagttg catagacagt caaacgattt tccttttttg atgaatcgaa gccgtgttta
     1321 atataatttt caacaaatgg ctgtaaagaa agttttggta acattaattg atcaatatga
     1381 tcaatcagct gatagtcaaa gttatgtcga taacgacgtt gatgtagttg caaggagagg
     1441 tcgatattaa ttaattcctc tttcaaatca attatgtcgg gctgatatgt attattgcga
     1501 tacaacttgc tcaataaata tatactgtcg ctgatttctt cgtcatcatt caatatggca
     1561 gtcattcgaa taaattccaa attattaaat agaaaatgag gctcgatttg atattgaagt
     1621 gctgcatact ttgcttcttg cttcaaccaa ttattttcat aaagtgtttt ttgatatgta
     1681 gcattatcta aataaaactg attgatatgc atagcgattt ctttcaaatc accgactttg
     1741 ttttcgatat tgattgactt gtagtctcct gtagatgaat gatttaagga agtaatgatt
     1801 tcctgatact gctggatata gttacgaaaa cttctattta ataattgatg aatagcaaga
     1861 ataaacaaac tcattaataa gaaaatcatg attgccagac ctaataaggt ttgtgacgaa
     1921 taatttggat tttcatatac ttgtaatgtg tggttaccga tagctatctg ttgcgttttt
     1981 ttccatttag aaggcgagat gtcttcagat aaattgacgg aatcaatacc attagacaaa
     2041 gaataattgt aatgtgttgg aaagagactc ttttttagcg cgttgatcga aacgtctaga
     2101 atcaatgtag catcgggcat gtcctcacta atgaaactta atgaacgaat aaaagaaatt
     2161 ttttcgtttt ttgtaggtgc gtaggcactt aagtattttt cgtaatctaa tgagatagat
     2221 atatttttaa cttcatcatc ttgcacaagt ttttttaagt tatctactaa ataggtaggg
     2281 gagcgatcct ttgacaaaat atcttccatg tattcagcag gtgatgagag gtagtagtca
     2341 ttaaggtttt cttggaaatc ataattttga tagatctctg tcacgatacc ggagagaata
     2401 cgattatatg tttccatata gtgggtgatt tgctgaatgt tttgcaggtt ttgatttttt
     2461 tctttttcaa tcacattgat ataggaaaag aacaatgtga aacaggcaag taaagtaata
     2521 aaaaaaatca atatgtatgt aaaacgcata ataacttttg ataaatcgtt tttcattcaa
     2581 agtcctcatt ttgataatct ctaattttat gaatcattat agcacatatt acaagtgatg
     2641 taaacgcttt gcaaacaggg ctacttatga ttgtgaatac agtttccgtt taaaaactat
     2701 tctattgtag aggtttaata aactaaaaat aatgtataaa aaattaatgt aagcgattaa
     2761 ccaagtgggt ataatcaagg aaacgctgag agaggagaga caaaatgaag ctaaagaaag
     2821 ggttattagg aaatctaaaa aaaaaccgag cattgtttat tatgctttta ccaggtgcgt
     2881 tatggttatt tatattttgc tatataccaa tgtttggagt tgtaatcgct tttaaagatt
     2941 tcagaatttc atcaagggga ttctttacaa gtttgttcaa cagtccttgg gttggctggg
     3001 aaaatttcaa atatctcttt gcttcgcaag atgcttggat cattactcgc aatacggttg
     3061 tttataacgt tgtaggtatt ttgctaagta ctttttgtac agtgtcatta gcaatcattc
     3121 tgagccaaat ggcgaacaaa cggttattga aaaagatgca gacatttatg atttttccta
     3181 attttttatc atgggtagtc atcagttact ttgtttacgc atttttgagt acggataaag
     3241 gattaattaa tattttacta agcaattttg gtgtggaacc cataaattgg tatagtgaac
     3301 cgaagtactg gccttttatt attgttttta cgagtttatg gaaaggcgta ggctatggat
     3361 ccatcattta ttacgcgtca attatgggga tcgatactag ttattatgaa gctgctttag
     3421 ttgatggtgc cagtaaatgg caacagataa ggaatgtagt cttgccacat ttaagatcga
     3481 ttattgtcat tatgttgatt ttgtctgttg gtagtatttt tcgagctgat tttggattgt
     3541 tttatcaatt gccaagaaat tcgggggctc tttatgatgt gacgaatgta ttagatactt
     3601 atgtctaccg tggattaatg aatagcggag atatcggaat gtctacggca gcgggattat
     3661 atcagtcatc agttggcttt gtcttgattt tgattactaa taagatagtc aacaagttag
     3721 atcctagttc agcact
//
LOCUS       DAKBVG010000208         3707 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000208 DAKBVG010000000
VERSION     DAKBVG010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.208"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(90..1211)
                     /gene="mnmA"
                     /locus_tag="OUD09_002392"
     CDS             complement(90..1211)
                     /gene="mnmA"
                     /locus_tag="OUD09_002392"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCT9693624.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNHAEVVFD
                     EPVRAITPGQAVVFYDEMECLGGGLIDHAYQETKVLQYV"
     gene            1615..2214
                     /locus_tag="OUD09_002393"
     CDS             1615..2214
                     /locus_tag="OUD09_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCT9693625.1"
                     /translation="MNYFTSDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(2364..3637)
                     /gene="efmA"
                     /locus_tag="OUD09_002394"
                     /pseudo
     CDS             complement(2364..3637)
                     /gene="efmA"
                     /locus_tag="OUD09_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
BASE COUNT         1136 a          819 c          643 g         1109 t
ORIGIN      
        1 taattttatt gcaaaaaaac agctatgcta tccaaaagaa aaggtgtttc ttttgttagc
       61 catagctgtt ttgattcatt taatatcgct taaacgtatt gtaaaacttt tgtttcttga
      121 tatgcgtggt cgatcaaacc accacccaga cattccattt catcataaaa tacaactgct
      181 tgtccaggag tgattgcacg tacaggttcg tcgaatacga cttctgcatg attatcatcc
      241 aataagcgaa ctgtcacagg aacgtcttgc tgacgataac ggaatttcgc tgtacacttg
      301 aattcttttg gcattggttc attcgtcgta aagtggattt cacttgcatc caagcttgtg
      361 gcatacaatg caggatgatg gaacccttgc cccacatata atgtgtttgt cgctagatct
      421 tttcctacaa caaaccaggg ttcttgcgaa tctcctccgc caccgatccc tagtccttga
      481 cgttgaccaa tcgtataata catcaagcca gcatgttgcc ctttgacttc accatcaaga
      541 gtgaccatat ttcctttttt tgctggcaga tagttgctca aaaattgttt gaagttcttt
      601 tcaccaataa aacagattcc cgttgaatct tttttcttag cagtcgcaag gcctgcgcgt
      661 tcagcaattg cccgtacttc tgatttttcc atgccgccta gagggaacat cgtttttgaa
      721 agctgttctt gtgataattg gctaaggaag taagtttgat ctttattatt gtctactcca
      781 cgaagcatgt ggaccacgcc gttttcatcg cgagtgactt gcgcataatg tccagtagct
      841 acataatcgg cgccaagatc cattgcataa tccaagaatg ctttgaattt gatttctttg
      901 ttgcacatca cgtctgggtt aggtgtacgt cctgcgcggt attcggctag gaaatattca
      961 aatacacgat cccaatattc tttttcaaaa ttcacagaat aataaggaat cccgatttga
     1021 gcagctactt tcgctacatc cttataatct tccgtggccg tgcatacacc gttttcgtcc
     1081 gtgtcatccc aatttttcat gaatattccg atcacgtcgt atccttgttc ctttaacaaa
     1141 agtgctgtta cagatgagtc gacaccgccg ctcatcccta cgacaacgcg tgttttgctg
     1201 ttgtctgtca tgatatcacc atcatttcaa ttagattctg aaacatctac gagttgaagg
     1261 tcgcaacttt tcagtatttt acattatacg tcatatttct gaaaatttca tcccttaatt
     1321 atcaagtcgt aaaaatactg ttctactgga cctattcagt acacaaacat gatagtcaac
     1381 caaaaaatca ctcatctttt gagcattcga tataaaaaat tggcatactc ataaaaaggg
     1441 ccagcctttc ggcaataagg aataatttta taaattgtga aaaactattt ttcaaaattg
     1501 cttcttatcg cttaaagcta cacgtctcat ttacaacctt ttctttttag atgttatgag
     1561 ttgctcagat aagtgatttt gtttactcta agaaaaaagg gaggcaaaag atacatgaat
     1621 tactttacca gtgatatgca ctttttccat gaacgcttat taggaaaaaa cgactttgct
     1681 ccgcggctct ttgcaaatgt ggatgaaatg aaccaacagt tgatcactag ctggaatgca
     1741 gtaataaaag aaaccgacca tgtttatcat ctaggcgacc ttgctatgca tccgcaatat
     1801 gaaaaagcaa atgcagaaat tttggaactg gtcactcaac tgcaaggaac aatccacttg
     1861 atcaaaggga accatgacag tcgcgctttt ttccgttatt tagaaaaaaa tgatcctagg
     1921 cttactgacg gaagtcccaa attttatttt tatgatgtag gtgccatcgt taaattcgac
     1981 catcaacaat attacttgac tcattatccg atgttgctcg ggcctaacga cactatccgc
     2041 aatcttcatg gacatatcca tcattacagc gtacctatcg agaacgatgt caatgtaggc
     2101 gtagacgcac cggaaaggga actcttaaag gaaaaactac catttggcac tccgttatct
     2161 gagcaacaaa tcaaagaaat ctatgaagca aagaggaagt acgataggtc gtgaaaaaac
     2221 tgccctatat caagtattaa acaaataaag ataattactc atatttttca tttgagtagg
     2281 cttattgcca caggaacatt aggatcagaa atggagttgt aacaagactt ctgtcacaac
     2341 tccattccat actattcgtc tttttactca tggtgttctt ttaccaattt tttctgtaat
     2401 tctttatcat aatttcgagc agctgacgtt aaaaataaaa cgattccgca taataaaatc
     2461 ccgattccag aaaacaagaa cattttttct acccctaaac gatcagctaa tggtcctgca
     2521 aataataatc caattggacc tgccaggttc atcaaagagt tgaagttccc taagacacga
     2581 cctaaaacat tcgattcata actctgctgg atcattgcca tcagcagtgt attgaaatat
     2641 ggcgttgcac aacctgctcc tgcattcaag ataagaaagt acaagaaccc ttgactatcg
     2701 tttggaccca atccgcttgc accgatcgtc aggcccataa tgatgtaggc tataataata
     2761 ggtttcattc ggtctttcca tgttcctata aaactgatca gtgcaccgcc taagagcatt
     2821 ccgactgcgt agactacttc cactgctccc gcctgtccaa cggaaccacc aaagtaatct
     2881 agcgtcatca atggatacag actgattgct ggcatgaaca acagcatcac aaatgcacca
     2941 ttgatcgtta tgtaccataa ccctttgtta tccatcaatt gctgcagccc aaattttgca
     3001 tctttaaaaa tagtaagcgg aacttccaat atttcaggag aaacttttgg gatcttcaca
     3061 aaaatcaata agccgacacc aaaaactgcc cccaatacat ctaataaaat aaggtaattg
     3121 acaggaacga ctgaaaacaa agccgcacct agagctggcg caatgatgaa attcgctgat
     3181 tggaccattc ctaactgtcc attcgtcctt gttatgtgag aagatggaac gattgtcggc
     3241 ataatcgact gatcgttggc atttggaatg tttgtgcgac cgaacgcata aacaaagaca
     3301 caaaaactag ccaaatcggg aaggattctg aaatcgttcc gactactgct aaaataagcg
     3361 cgaaaatcgc tacaataata tccgtgacaa tcaaaagagc ttttttatcc cagcgatcca
     3421 ctagcggttc tacaaatggg cttagtaaaa ccatgggaat cattcctagt aaagtagcaa
     3481 aactcaaaat tgtcgccgaa ccggtttctc ttgtcaaata ccagatgatc gcatattgga
     3541 cgaccatact agtgattcct gacaaaaact gtcccgataa aaagaacaaa taatttctct
     3601 tccaattcgt taaaacgacc gactgttcat tttccataaa catccctctt ttctttttca
     3661 ttttgtatag cgtttgcaca acaatcatta tacgggattc ttttttc
//
LOCUS       DAKBVG010000209         3636 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000209 DAKBVG010000000
VERSION     DAKBVG010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.209"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            110..574
                     /locus_tag="OUD09_002395"
     CDS             110..574
                     /locus_tag="OUD09_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCT9693626.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            588..1337
                     /locus_tag="OUD09_002396"
     CDS             588..1337
                     /locus_tag="OUD09_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCT9693627.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            1337..2167
                     /locus_tag="OUD09_002397"
     CDS             1337..2167
                     /locus_tag="OUD09_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCT9693628.1"
                     /translation="MENSTEKKRLITKKDLHKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTPFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            2167..2814
                     /locus_tag="OUD09_002398"
     CDS             2167..2814
                     /locus_tag="OUD09_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCT9693629.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKIDYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(2912..>3636)
                     /locus_tag="OUD09_002399"
     CDS             complement(2912..>3636)
                     /locus_tag="OUD09_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCT9693630.1"
                     /translation="NDIREKKYLPGEQLPTEEELCKIYGVSKITIRKAFKLLTESGTV
                     ERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTVLHSKIIKADEFLANKLQI
                     NVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGSQYTSLYKLLEDDYDVKAT
                     SSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQPFHYSQSTVRCDRISYVI
                     EVTENITLDTVHKNDSDPKVID"
BASE COUNT         1205 a          502 c          656 g         1273 t
ORIGIN      
        1 ctgaataact ctcaaaaaag tatggttcat attaattcgc ttttaaaaga tgtcagttaa
       61 ggtggcaatt tctattctat aaattttaaa aattgaagga ggaaatagaa tgattaaatt
      121 agtgcgtgta gaccacagat tacttcatgg acaagtaata ttttcttgga ctaaacagta
      181 tgatattaac catattattg tggctgataa caaagttcca ggagatccaa tgtctgtgat
      241 ggctttgaca attgctaaac ctgctgattg caaattagac attgttaaaa ttagtgatgt
      301 taaagggatt atggaaacat ccccaaatga taattacatg attttaatta aaggacctaa
      361 ggaagctttt gaattagttc aaaatttacc agaaattaaa gaaattaatt ttggcggtgt
      421 gtctaagaaa agcgagtcca aacaatatgg aaaggcagtc tttttaactc ctgaagaatt
      481 agaagccaca aaaaaaattc ttgatcaaga agtgaaaata tttgtgcaac aagtcccatc
      541 ttcaagtatt gaaaatgtag attttagtaa gtaagggggg aaaataaatg ttattaaaag
      601 ccattttatt aggtttagtt ggtgtggtag ctgttattga ctccaggtta attggtcgac
      661 aaaatattgg tagaccacta attttgagta cacttgccgg acttgtgcta ggtgacttgc
      721 atacagggat tgttcttggg gcatctttag aattaatttc tatgggattc gtctcaattg
      781 gggcggctgg acctcctaat atgcaacttg gtagtattat tgccactgct tttgcaattt
      841 taactaatag tagtacagaa acagccttaa caatagctat cccagtggct gtagctggtg
      901 aatttatgag tattatcatg cgtatgataa ttgctcaatt tggacatgct gcagataaag
      961 ccattgaaaa taataaattt agaaaagctc aaattattca tatttattgg tcatttattt
     1021 ttaatgcttt tgtttacttt attccaatct ttttaacagt atattttggt gctgacgttg
     1081 tggcagattt agtagataaa attccagaaa tgattacaga cgccttgact gtctctggaa
     1141 atatgctttc cgcattaggg tttgcaatgc tattaagcac aatgttgagt aaaaaattat
     1201 atccttactt tatttttgga ttctttattg ttgcctattc aggtctttca ctaattggcg
     1261 tgacaatttt tgcagctctg attgcctttg taatggatca agttaaatat ggaaaaagag
     1321 aagaggcgca tggttaatgg aaaatagtac agaaaaaaaa cgtttaatca caaaaaaaga
     1381 tcttcataag atgtgtcttc gttcatggac aatcatgtgt tcgtggaatt atgaaagaca
     1441 aatgcatatg ggatttatgt atgggatggc gccaatttta aataaagttt ataaaaatga
     1501 tgaagagaaa aaaagagaag cttatactcg tcatatggca ttttttaact gtacgcctca
     1561 aatgactcca tttattatgg gactttgtgc ttctatggaa gaacaaaatg ctcaagcaga
     1621 agaaggaaac tttaatactg aatctatctc catgattaaa accagtttaa tgggtccttt
     1681 tgctggaatt ggtgattcat ttttccaagg tactttaaga attattacat ttgggattgg
     1741 attgtctttt gctaagcaag gtagtatttt aggaccgatt ttagctgttg gcctttttgc
     1801 tataccttct attttagctg ttttttacgg cggtaaatta ggatatgagt ctggcaataa
     1861 ataccttact aaattgtatc aagaaggtct tatggatcga gtaatgcaat ttgcttctat
     1921 cattggtttg gctgtagtag gtggaatggt ggctagcatg gtgccaattg tcaccccttt
     1981 aaaattcact acagggggaa cagaacttgt tttacaagac ttacttgatt ccattattcc
     2041 tggattatta ccacttgtat gtactttggg aatatataaa ttgattaaga aaaatgttag
     2101 tacaaatatg ctcttattag gttgtatttt ggcagggttt gttttaagta taataggtat
     2161 cttataatga ttaaggctct aatttttgat atggacggag ttttaattga tagcgagatt
     2221 atgtatcaaa agtgggtatt taatttttta aaacaagaaa atttaagcta tcctttgtat
     2281 tcttatcaaa aaaagattgg aactacagca agtatttttg atgatataga aaagtacaat
     2341 gagggatgta acagtttaga attgaaggga aaatttacta aatattggaa atcgcaaaag
     2401 attgattact tggatatttt tcctaatata ctaattgaac agcttgaatg gttaaaaaag
     2461 aaaaaaataa aaatggcaat tgcttcttcc agtccaatgg atactataaa tgaagttgtt
     2521 accacttgta aaatagagtc atattttgat tgtttaatta gcggaagaga tttgccagaa
     2581 agtaaaccta atcctactat attccttaaa gcttcagaac agctcagaat tccagttgaa
     2641 gaatgtctgg tgatagaaga ttcttataat ggtataaaag ctggtaaaag agcaaatatg
     2701 aaggttcttg caattaaaga taaacgtttt tctcaagatg taagtctagc agatgatgtt
     2761 atttacgata ttaaatatct gcgacaagaa gtacaggtga aatttggaat ataaataagt
     2821 tttttgatgt atttagagaa tattagtaaa aataaaaatc atgcccaaat ctaatcaaaa
     2881 ttgattgctt tgggtatgat ttcttttaat atcaatctat tactttagga tctgaatcat
     2941 ttttatgcac cgtatctaaa gtgatatttt ctgtgacttc aataacataa ctaattctat
     3001 cacaacgaac tgtggattga gaataatgaa atggttgatt atttttatct ttgccaacct
     3061 tttctaaaat aaatagcgga tctcctacta tacattgcaa taagtcggaa agctcaggtg
     3121 ttgcagataa accgtttatt tccataatag agctcgtagc tttgacatca tagtcatctt
     3181 ctagaagttt atataatgaa gtatattggc tacctttttc aaataaagtt ggaaattttt
     3241 cttctgatgc gaaaaaatta tcaattccca tcggggcacc gtcttcccac attaaacgtt
     3301 taatattgta aactctatca gttacattga tttgtaattt attagctaaa aactcatcag
     3361 cctttataat tttagaatgt aaaacagtat atctaatttt atgtcctcgg gtagaaagag
     3421 aatcgctaaa tccatgaact ccagctaatt ttaaactttc ttttttttga gttacaaagg
     3481 tccctttccc ccttaatcgt tcaactgttc cactttctgt taaaagttta aaagcttttc
     3541 gaatggtaat cttacttact ccataaattt tacataattc ttcttctgta ggtaattgct
     3601 ctcctggaag atattttttt tcacgaatat cgttac
//
LOCUS       DAKBVG010000210         3607 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000210 DAKBVG010000000
VERSION     DAKBVG010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3607
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.210"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(231..908)
                     /gene="nth"
                     /locus_tag="OUD09_002400"
     CDS             complement(231..908)
                     /gene="nth"
                     /locus_tag="OUD09_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCT9693631.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(915..1613)
                     /locus_tag="OUD09_002401"
     CDS             complement(915..1613)
                     /locus_tag="OUD09_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HCT9693632.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            1881..3065
                     /locus_tag="OUD09_002402"
     CDS             1881..3065
                     /locus_tag="OUD09_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HCT9693633.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3071..3514)
                     /locus_tag="OUD09_002403"
     CDS             complement(3071..3514)
                     /locus_tag="OUD09_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCT9693634.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
BASE COUNT          929 a          722 c          606 g         1350 t
ORIGIN      
        1 gaatactgga cttattttat aggtatattg gaaggcatta ttttctacat cgggtatttt
       61 cccaataaaa ataacaactt ttatttttat cgcaataaat tgtaataagt taccatccta
      121 gctactgagt atgtacattt actttttcat gcaaaaaaga taaacgaaac agcttttctc
      181 cataataaca agataattca tacatttatt tgcaattaaa gaaaccacgt ctacaggcgt
      241 ggtttctttt ttagcgcctt ttcttttaga cgcatccgat ttttcccatc ttgacatatt
      301 gatagcaaag gacatacctc gcacttggga tttcttgccg tgcaatgata tctaccaaaa
      361 aaaatcagtg tatgatgtgt ctttacccat aattcttgtg gtactttccg catcagtgtt
      421 tcttctactt ccatgaccgt agcatctagt ttgcagatcc tcaggcgttt tgagacacgt
      481 tccacgtggg tatctactgc gattgctggt atcccgaatg catcccccaa tacgacattt
      541 gctgtctttc tgcctacacc aggaagagac atcaattctt ctcttgacgt tggcacttgc
      601 ccatcgaaac gttcaatcag ctgctgtgca cacgctttga tgttttttgc tttatttcga
      661 tacaaaccaa tcgtttttat tttaagaatg atttcatcga ttgaagcttc tgctaacgca
      721 tcgggtgtcg gaaaagaggc aaacaaatct ggcgttgctt tattcactga tacatctgtc
      781 gcctgggcac ttaagatcac tgcaatcaat aactcaaatg gattattgtg cttgagttct
      841 ccatgtgctt cgggaaacat tccatacatc gtttctagag cttccattgt tttttgtttg
      901 cttaacatat tccgctactc ctccaaccaa tttttcagtg agactttcgg caattcttct
      961 tgttgagctg actgttctac ttctctttga agaagctgct gttttcttcg tttttggtct
     1021 tcttccactt gttgttttgt acgtagattt tttctttccc acgatagcaa aatgcggtca
     1081 acgtaattaa aactataagc ctgatttaat acggcttccc gcagtgctag cttcaaaatt
     1141 tccggctgat aatggtcttc ttctagccat tgtccgatcc gctggaactc gatcgaagaa
     1201 agcgggcgtc caaattcttg ttcaaataat tgatacactg actggatttc tttttcttgt
     1261 gttttttctt cgtatttttt ttcttgtgtt tttaaatact cacctaatag atcatagatg
     1321 gggtaaagat tatatcgatc tgctttttta ccgttgacaa tcgtcgtttc tatcttgata
     1381 aatccatttg ttacgagacg atccagtatt tgaaaaatct gatcttgttt gattcccata
     1441 tccatagcaa tcatctgcaa atcagggaaa gtatctcctt caagctgtgc catatgcaac
     1501 tgtagaacaa ataaaaattc ttcagattgt agaccgatcc tatgataatt tttaagtaaa
     1561 agattagata tgactgtttg tccagcgctt aagtaatttt ctaaattcaa catgatttct
     1621 cctccgctca atcacagtat acgtaaaaaa aacatgggaa gcaagaacag gtttcttttc
     1681 tatttacttc actgatcggt cagatttaca atgtcgtcta cttgattttt gtttgttcat
     1741 gtttttgatg cgtatcttca cttttcggaa taaataaatt aagtagattt cgctgttcct
     1801 tagaatgttg acaaggaaac aaaattacta tacactattt ttgtttccaa gttaacatta
     1861 ttgcataaag gagtttttac atgtacagtt tcacaaataa acattctaaa gtttttggat
     1921 acgtctctgt cttattcact ggattaggat ttactatcgt cagtcctgta cttccgtttt
     1981 taacgctgcc ttattctcac tccatacatc agcaagcttt ctatatcact ttgctcatgt
     2041 ctgtttatgc tctagctgcc ttcttatctg cccccattct cggtagctta agtgatcatt
     2101 ttgggcgtca tccgatcctt attatcagtt tattaggatc aagtatcgga tatttgcttt
     2161 ttggtttagg aaattcgata tggatgttgt tccttggtcg tatcattgaa ggattgacgg
     2221 caggagaaat ctccacctta tatgcttact ttgccgacat cacggaacct aatgaaagaa
     2281 ccaaagtttt tggatggatg ggtgctttag tcggtatagg aacaacgctg ggtcctatta
     2341 tagggggtct attagctgag ttggggaatt ctgttcctat atttattgga gcactcttta
     2401 ctttcttaaa tgccgtttat ggctacacat ttatgctaga aagccttcca ataaaaaaac
     2461 gttcagtaga tttaagtttc tcccatgtaa gaccttttca ccaattacaa caattattca
     2521 agatttcatc cgttattcct ttattgacag caggttttgc tgtttggttg gcagctggct
     2581 ctcttcaatc tatcttttct caattttcga ttgacgcctt tcagtggaag gctgggttgg
     2641 ttggtttaag cttctctttg attggaatct tggatactgt ctcgcaattg ttcattatgc
     2701 ctcgattgct caaaaaattt tcagaacagc agatcacaag aatagggatg ttcagtgaaa
     2761 tcctcgccta tctattcatc acattatctg gaattcttct actgccgatt ttgtttttat
     2821 ttgggattat ctgttatggt ttcggtgatt ctatttttac tcctgtattc aatggccaac
     2881 tatccaattc tgtctcagat aaccagcaag gtcttgtaat gggcggtacc caaagtatac
     2941 aatctctatc gcgcgtcatc ggcccgctaa tcgcaggaca gctttatgct gcagcaccat
     3001 ttcttccgac ggtattcgga tttttgctta tgatttttgc ctattatcac tacaataaaa
     3061 aatagtagct tcatttgcta cttcgtataa attctctgta tcgaccgata aaacgaaata
     3121 ctgtgtcaaa ttcttctttt gacatatttt caaatacagt agcatctcgc tgtacccatt
     3181 tttgatggag ttgctcgtgt ctattcctta ttttttgtcc gattggcgtc aattgaaaat
     3241 agatttcttt tttattctct tcgctttgat aagtgtttat gatccctcta gctatcagtt
     3301 tttttgttag cttactgatg gctcctctag tcatgtaact agcattcgct aattttgtca
     3361 cgttaggatc agggattttc ccgatatgtt caatcacttc tagttcattt agagtaaatc
     3421 ctgcaaattc tttttccatt tctggtttat tacgatgaat aatctcatta gctaaatcca
     3481 gatattcttc tattagcagt tgtcttttat ccatttctac accttctttt tctttcatta
     3541 tattgttttt ttgtttccta ttcaacaaag ataaaaatat tttgtaggaa aacccaatct
     3601 gaaacca
//
LOCUS       DAKBVG010000211         3604 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000211 DAKBVG010000000
VERSION     DAKBVG010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3604
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.211"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            <1..854
                     /locus_tag="OUD09_002404"
     CDS             <1..854
                     /locus_tag="OUD09_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCT9693635.1"
                     /translation="TEKFPKQDQDTPALQQNMIPVPDCGEESYEGNNQLENRRVLITG
                     GDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGRKIVLLPFDLRDEQA
                     PKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFMINIIAMFALVKEAV
                     PHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQLAPKGIRVNGVAPG
                     PIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLASNKASYVTAQIYGVT
                     GGEAINL"
     gene            892..1455
                     /gene="amaP"
                     /locus_tag="OUD09_002405"
     CDS             892..1455
                     /gene="amaP"
                     /locus_tag="OUD09_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HCT9693636.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            1469..1657
                     /locus_tag="OUD09_002406"
     CDS             1469..1657
                     /locus_tag="OUD09_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HCT9693637.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            1670..2572
                     /locus_tag="OUD09_002407"
     CDS             1670..2572
                     /locus_tag="OUD09_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCT9693638.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            2604..2846
                     /locus_tag="OUD09_002408"
     CDS             2604..2846
                     /locus_tag="OUD09_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCT9693639.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(3102..3473)
                     /locus_tag="OUD09_002409"
     CDS             complement(3102..3473)
                     /locus_tag="OUD09_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693640.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         1300 a          635 c          736 g          933 t
ORIGIN      
        1 atacggagaa atttcctaaa caagatcagg atacaccagc tttacagcag aatatgattc
       61 ctgttcctga ttgtggagaa gaatcctatg aaggcaataa tcaactagaa aaccgccgtg
      121 tactaatcac tggtggagat tcagggatcg gtcgtgcagc agcgattgca tttgctagag
      181 aaggggcaga tatcgctctt caattcttcc caggagaaga aaaagatgca gaggaagtag
      241 caaaatacgt gaaagttgca ggacgtaaga ttgtcttact accttttgat cttcgtgacg
      301 aacaagcacc taaagaaatc atagataaag ccgtgagtga acttggcggg ctagatacga
      361 tggtactaaa cgcaggacag caaatttctt gtcagtctat tgctgagtta ccacttgagc
      421 aagtcaaaga cacatttatg ataaacatca tcgctatgtt tgctttagtg aaagaagctg
      481 ttccgcatat cccagcagga ggagcaatcg taacaacaac ttctgttcaa gcattcaatc
      541 caagcgaaca tttattagat tacgcagcaa caaaagcttc aatcgctaac tttacgatcg
      601 gtttagcaaa acagcttgca cctaaaggaa tccgtgtaaa tggtgtagca cctggtccaa
      661 tctggacacc attacaacta gacgaaggac aaccttctga ttcgatccct gaatttggtc
      721 agcaaagttt actagaacgt gcaggtcagc caggagaatt agcaccagtt tatgttttct
      781 tagcatcgaa taaagcaagt tatgttacag cccaaattta cggagtaact ggtggagaag
      841 cgatcaactt ataagaagtt gatcgtttca cccctatgag gaggtgcgaa aatgaataaa
      901 ggtgtaaaaa cgatcggagt gatcggaaca ttacttttgc tatctgtgct tttgtttgcg
      961 atactattaa gtgcaaccta tccattgcct tttcagctag agaagtttcg attcttttcc
     1021 atcactaatt catacgtgca acaatatgta ttttggcttg ccgttgcatt tgctattttg
     1081 acaattatca cgttacttgt tttcctgttt tatccaaaaa caatcggaaa ctttctactg
     1141 aaacatggtg atggggagtt gacgttggat aaaaaagcaa ttgaaggaat ggttcgttct
     1201 cacttgcatg aagatgaatt catccattca ccaaaagtaa atgtcaaagc aacgaaaaac
     1261 aagatcgccg tcaatgtaaa aggggagttg aaacgaactt cagcactcat tggtaaaaca
     1321 ggaacattga tgaaagaaat cgaaaatgaa gtacaacaca tattgggaac aaaagagcca
     1381 gtgaaagtat cggtgaaata cacaggcttt gaagaaccag ctaaacgaag caatgaccgc
     1441 aaacgagtag aatagggggg gaccaaaaat gaaagaacta ttttcgacat acaagctccc
     1501 tatcatcttt ggggtacttg gattgatatt agccgtttta tggatcaccg tgggcttttt
     1561 caagacctta ttgatcttga tcatggcagc gatcggcgtc gctatcggtt tttatctgaa
     1621 acaaacgaaa atcttagatc actattttaa atcttaggag gaaacatcca tgaataacaa
     1681 taacaaaaat aataacaata aaatgaacaa ccaaaaacca gtgtctgcac atccagaccc
     1741 aacgacacca aaccaatcaa atggtgtgaa cgatgaaaag aacaaaacag caccagtatc
     1801 tgcacatcca gatccagtag tccctaacgt tcaacaagga gtagaagaga agaaacatga
     1861 tgcagaaaag catgcacctc atcatccagg aactccagaa tctaaagttc cagaacaaca
     1921 tgatgcaatc gatccttcta aacctgtgga agagcaaaaa catccttctg accatgcgaa
     1981 agcacaagaa aaacttagtt ctgaccacac aaacggacaa ggaaaacttg gttctgatca
     2041 tacaaataac caaggaaagc atgattctga acaggaagat tcaatgcgcg gtgagctgaa
     2101 ttatgaagat aaagttgtgc aaaaaatcat cggaatcgca attgaacaag tagacggttt
     2161 attatctgca aacggtggtt tcttctctaa tgtggcagga aaacttgtga atacagataa
     2221 tgtaacggct gggatcgaaa cagaagtcgg taaaaaacaa gtagcagttg atatggatgt
     2281 cattgtggaa tatggcaaag atatcgaaaa aatctttgaa gaaatgcaag aagtaatcgg
     2341 caaagaagta aaaaatatga cacatttaga agtgatcgaa gtcaacgcta acgtagtgga
     2401 catcaaaaca aaagaagaat ttgaaaaaga tcaagaaacg gtgcaagaca aagtaactga
     2461 cgcagcgaaa aaaaccggtg aatttgcttc gaatcaaaca gataaagtaa aatcagcagc
     2521 tggaagcggc gcacaaaaag taaaagaaaa tacagaacca cgggttcagt aataaggaag
     2581 taaaacatta ggaggaacga aacatgggat ttatttggtc acttatcgtc ggaggaatta
     2641 tcggagcaat tgcaggagca atcacgaata aaggttcttc gatggggatt attgcaaatg
     2701 tcgtagcagg tttagttgga tcagcacttg gtcaagcatt attaggttct tggggaccaa
     2761 gtattgcagg aatggcaatc gtgccatcat tgattggtgc catcatttta gtactaatcg
     2821 tatcattctt tgctcgaaaa ctgtaaaaag acttttaaaa agaggtcgtg aaaaagctgt
     2881 tcttcatcaa gtaataagaa cagccaaaca aaaatagctt ctcatatttt tcatttggtt
     2941 gggcatatta tcgaagatgc aagcttttga acactgttta tacggaataa gaggctgtga
     3001 caagactttt gtcacagcct cttatttatg cgggcaaatt cccaattaga tttttaaaag
     3061 tttttcctta aatgtgtctt tattgataca gagaagcttc tttaataaaa caatgcctcc
     3121 ctaaattttc ttgagatagg aaaggtgaac atacaaaata atacgaccac tttgaaaacc
     3181 cagatgatcc acaaatggat cgtatgatac tgatgaagta aaaagcaatg gatcagaaac
     3241 tcaaagagat tgaaaaagac aaggaagaaa taatatctct tttttactct tgtctgtttt
     3301 agccaataat agctcgtaaa gtaatctaga caaacaaata gaagtaaaca aagtttcgta
     3361 agagcagcaa tcgtatcatg ttgctctgct agacgattga cgagcaggaa aaatgttggt
     3421 aatggaatat agatcacgag taatgaaaga aaaaagtaaa caataaatgg catactattc
     3481 ctcctaattg tgtgagttcg ccctcatttt aaaataacga tatttgtttg tgaagctttt
     3541 tcgtgtcgta acaaatgtta tctaatgtat ttttaaataa ataatcatta ttatcaaaat
     3601 aatg
//
LOCUS       DAKBVG010000212         3576 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000212 DAKBVG010000000
VERSION     DAKBVG010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.212"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            156..401
                     /locus_tag="OUD09_002410"
     CDS             156..401
                     /locus_tag="OUD09_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693641.1"
                     /translation="MSKSKKNITIIGLGLTVYLFTEFVYRHVNLFAEPLFVLYESNSS
                     IVSELANLMIALLFTPALRVITILVCLVLLFFVNKKK"
     gene            complement(585..1334)
                     /locus_tag="OUD09_002411"
     CDS             complement(585..1334)
                     /locus_tag="OUD09_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCT9693642.1"
                     /translation="MLKLERHQKILDLLNAEQKVIASDLSQRFNVSEDTIRRDLKDLD
                     SQGLLKRVHSGALRVGPPITNFNYRETVASEQKTALAHKALPFLKEESVIIIDGSTTN
                     LALAKEIPQDFRATIITNSPPIAIALAQHPNLEIINIGGTFYKQSMINIGVQAYKQLQ
                     NIRADLYIMGIYNIDLDVGTSVPTMQESDIKREMIRISTEVLGMITNDKFGTISNHIV
                     GPIEDLTYLISEEIPEKVRKEYNKQKIVLID"
     gene            1520..2224
                     /locus_tag="OUD09_002412"
     CDS             1520..2224
                     /locus_tag="OUD09_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCT9693643.1"
                     /translation="MEKKIILSPSLMCGDLANLEGSIQELESIGMDSLHIDVIDGSFS
                     PSMPLGIETIKRIREITSMNFDVHIMAKDNDNEFFIGEMLKIGVESITFHYETSLHID
                     RYIQLIKNSGTKVGIALNPATPLNVLQYILPEIDRICLMLINPGFATNKSEKQVSYAL
                     KKITDLCQLVNEKELAVDIQVDGRVSLETIPGLLDAGATNLVLGSTSLFLKGNTLAEN
                     QKLLYQAMKQSVRKNQ"
     gene            2264..2821
                     /locus_tag="OUD09_002413"
     CDS             2264..2821
                     /locus_tag="OUD09_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCT9693644.1"
                     /translation="MSYKAAKQNIVREITETLDRISSKDVEQLIKKIEKAERVFFVGV
                     GRVLLALEGMAKRLGHLGIQTVVVGQITEPAITDKDLLIVGSGSGESAFPLTIAKKAK
                     EFGSQVAHIGSNPDSTMKEYSDLFIRIPVQTKLALPEEISSIQPMTSLFEQSVLLLGD
                     SIALVMIEEKAIDMSKLWQYHANLE"
     gene            2829..>3576
                     /locus_tag="OUD09_002414"
     CDS             2829..>3576
                     /locus_tag="OUD09_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCT9693645.1"
                     /translation="MKAVHFGAGNIGRGLIGQILHQNGFDLCFIDTNPELIHQLNQDK
                     GYTIEIIDEMNRSDFIDHVVALNSLSELEEVITATKNADILTTSVGAINLVKIAPIIA
                     QALSKRFEQKHSINIVANENVINASDVLKKEIYSLLSEKEQENFDEYAYFVNTAIDRQ
                     SLGKVLGGKMISVVEPYYEWIMDRQQLDPTTSFDMKNVNLVDDMEPYIERKLYIVNAA
                     HAAVAYLGALKGYKTIQEAIQNMEIFNLVKQ"
BASE COUNT         1216 a          515 c          705 g         1140 t
ORIGIN      
        1 agtcatgtat atggtggtca aaaagtaaat aatatcagtt atcaaatttc ctgtaaattt
       61 tcttgaaact acagcatatt attacttcaa aacaatctac attttttata aaaaaggaaa
      121 tgaataactg aaaaaattat tataggagag aaaaaatgtc aaaatctaaa aaaaatatca
      181 ccataattgg tttaggctta accgtatatt tatttacaga atttgtttat agacatgtga
      241 atttatttgc agaaccttta tttgtattat acgaatcaaa ttcctcaatc gtcagtgaac
      301 ttgctaattt aatgatagca ttgctgttta ctcccgcgtt gagagtcata actattttgg
      361 tctgtttagt gctattgttt tttgttaata aaaagaaata acgtagtatt tcaaaagaca
      421 aagaagtggg atgatgattg ctatagtatg ttatacgtgg tgctaggtaa gtgagaataa
      481 acaaaaaagg aaacacgcaa taaagtttat ttcaaaactt tattgcgtgt ttctttttta
      541 gattttttat atctgttgct ttagtagtta ccaatagttt tcgcttaatc aatcaaaacg
      601 attttttgtt tattgtattc tttacgaacc ttttcaggaa tttcttctga aataagatag
      661 gtaaggtctt caattggacc gacgatatga ttactgatgg tgccgaattt atcattagtt
      721 atcatcccaa gtacttcagt agaaatacgt atcatctcac gtttaatgtc ggattcttgc
      781 atagttggca cgctcgttcc aacatctaga tctatgttat aaatacccat aatataaagg
      841 tcggctcgaa tgttttgtag ttgcttataa gcttgtaccc caatattgat catagattgt
      901 ttataaaaag taccgccaat attgataatt tccaaattag gatgctgtgc taatgctatg
      961 gcaatcggcg ggctattggt aatgattgtt gcacgaaaat cttgtgggat ttcttttgct
     1021 aaagccaagt tagttgtact tccatcaatg atgatgacag attcttcttt taaaaaaggg
     1081 agggctttat gagctaatgc agttttttgt tcgctagcta cagtttctcg gtagttaaaa
     1141 ttggtaattg gcggaccaac acgtaaagcg ccgctgtgta ctctttttaa gagaccttgg
     1201 ctatccaaat ctttcaaatc tcgtcgaatc gtgtcttcgg atacattgaa acgttgactt
     1261 aaatcactag cgataacttt ttgttctgcg tttaataaat ctagaatttt ttgatgacgt
     1321 tctaatttca acatgttgcg tgctccttct tatttattct tgtttatcat gtcatttatg
     1381 cgtttttatg tcaataattt ccgtttttaa tacactaaaa aggcataaac aagcaaaaac
     1441 aaaatatttt tatgcagttt tatgttgact agtatttgtt aagcggttac aatgaggggg
     1501 taattaaagg agggaatgta tggagaaaaa aattattttg tcgccttcac ttatgtgtgg
     1561 ggatctagca aatttggaag gaagcatcca agaactagaa agtattggga tggattcctt
     1621 gcatattgac gtgattgacg gttcttttag tcccagtatg cctttaggga tcgaaacaat
     1681 caaaagaatt agagaaatca cttcaatgaa ttttgatgta cacatcatgg ctaaggataa
     1741 cgataacgaa ttttttattg gagaaatgtt gaaaattggc gtagagagca ttacttttca
     1801 ttatgaaaca agcctccata ttgatcgata tatccagctc atcaagaatt ctggaacaaa
     1861 agtagggata gctttgaatc cagcaactcc gttaaatgta ttacaatata ttttacccga
     1921 gattgatagg atttgtttga tgctgattaa tccaggtttt gcaacaaaca agtcagaaaa
     1981 acaagtttct tacgcgctaa aaaaaattac agatctttgc cagttagtaa atgagaaaga
     2041 gttagcagta gatatccaag tggatggacg ggtatcgtta gaaacgatcc cgggattatt
     2101 agatgcaggc gcaacaaatc tggtattagg aagtacaagt ttgtttctta aaggaaatac
     2161 attagcagaa aatcaaaaac tactttatca agcgatgaag caatcagtta ggaaaaatca
     2221 atagatgtag tggtcctgaa taatagaaat ggaggggaaa tagttgagtt ataaggcggc
     2281 gaaacaaaat attgtaagag aaattactga aacattagac cgtatttctt caaaagatgt
     2341 ggaacaattg ataaagaaaa ttgaaaaagc ggaacgagtt tttttcgtgg gggttggcag
     2401 agtactgtta gcgctagaag gaatggcaaa acgcttaggg catctaggaa ttcagacagt
     2461 agtcgtagga caaatcactg aaccggcaat tacagataaa gatttattga ttgttggttc
     2521 tggtagtggg gaaagtgctt tcccactaac tattgcaaaa aaagcaaagg aatttgggtc
     2581 ccaagttgcc catataggat caaacccaga cagcacaatg aaagaatatt cagatctttt
     2641 tattcgtata cccgtacaaa caaaattagc gttaccagag gaaatttctt ctatacagcc
     2701 aatgactagc ctgtttgaac aaagtgttct tttgttagga gatagtattg ctttagtgat
     2761 gatagaagaa aaagcaattg atatgtcaaa gttgtggcaa taccatgcta acttagagta
     2821 ggtgaaaaat gaaagcggta cattttggag cagggaacat cggcagaggg cttattggac
     2881 aaattctaca tcagaatgga ttcgatctat gttttattga tacgaatcct gaactgatcc
     2941 atcaattaaa tcaagataag ggttatacta tcgaaattat tgatgaaatg aacaggagtg
     3001 atttcattga tcatgttgtg gcactgaata gtttatccga gctagaagag gtgattacag
     3061 caacaaaaaa tgcagatatc ttaacaacat ctgttggcgc aataaattta gtaaaaattg
     3121 caccgatcat tgcgcaagcc ttaagtaagc gatttgaaca aaagcatagc atcaatatcg
     3181 tagctaatga aaatgtgata aatgcaagtg atgtgttgaa aaaagaaatt tattctttgc
     3241 tgtcagaaaa agaacaagag aattttgatg aatatgcata ttttgtgaat acggcaatcg
     3301 atcgacaatc tttaggaaaa gttcttggag gaaaaatgat ctctgttgtc gaaccttatt
     3361 atgaatggat aatggatcga cagcagttag atccaactac atcttttgat atgaaaaatg
     3421 taaatttggt tgatgatatg gagccttaca ttgagcgaaa gctttatatc gttaatgctg
     3481 ctcatgcggc agtagcttat ttaggcgcac tgaaaggata taagacgatt caagaagcaa
     3541 tccaaaatat ggagattttc aatctagtca agcaat
//
LOCUS       DAKBVG010000213         3572 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000213 DAKBVG010000000
VERSION     DAKBVG010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.213"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            60..611
                     /locus_tag="OUD09_002415"
     CDS             60..611
                     /locus_tag="OUD09_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCT9693646.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(764..1237)
                     /locus_tag="OUD09_002416"
     CDS             complement(764..1237)
                     /locus_tag="OUD09_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCT9693647.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            1375..2847
                     /locus_tag="OUD09_002417"
     CDS             1375..2847
                     /locus_tag="OUD09_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HCT9693648.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            2844..3554
                     /locus_tag="OUD09_002418"
     CDS             2844..3554
                     /locus_tag="OUD09_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HCT9693649.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTGGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
BASE COUNT         1146 a          616 c          722 g         1088 t
ORIGIN      
        1 ttttcgatta ctcatttatg atagaatgga tttaaagatt tgtcaaggag ggaaaactga
       61 tggcaagaaa aaatacactt cgatatattt tgttagggtt attaagtaag aagaagatga
      121 caggatatga attgaatcaa tcgttcaaaa atgaaatagg agaattttgg caagcaaaac
      181 atagtcagat ttatcctgaa cttgcaaaaa tggaagagca aggaatcatt cagcatcaag
      241 tagagatcac aggtgaaaaa ttggaaaaga aagtttatga attaacagaa gaagggaagg
      301 aacaattggc agagtggatt catacaccca ccaatgaact accggttaat cgagacgaat
      361 ttgttctgaa attgtatttc gtcaaagacg tgaatgatcc ggcattagta gaaattattc
      421 agcagcagcg tgaccttcat gaagaaaaac gacttcatct attagagcga aaaaaaacga
      481 tctttcctac tgaaaaagaa aaacatgaaa attacggaca ctacctgatt ttagcgcatg
      541 cgatcaatcg ggaaacagaa tatgcaaatt ggctaacaga tgtattagaa gaaagtaacc
      601 gaaaaaaata aaaaaatatc cgaacagtga tggagaattc ctttctatat gaaagaaacg
      661 cagatcaccg ttcggatatt cttatgttca aacaggcctt ttttcaagta tttatgtagc
      721 atctacttat taaaaagctt ggtacttagg agattagtgg cgtttagagt tcttttaaca
      781 actgttcgta gtcagctttt tcatcgacag gaaggagctt gtaagttaat tcgtctgtct
      841 cgttgtcatg aatgatgtac atcttagcat tctcttcttt gaagttaccg atttgtaaaa
      901 tcgtatagcg ttcgtcgatc aattcgatat ctttgattgc ttttgaaaaa actttacgga
      961 catggtgcat tttcttttgg cggatttctg ccttttctgc agcttttgac aaagtaaatc
     1021 cgtcatctaa atattttttg atcagttcta cttttatgac tgtaggtaaa cgatattttc
     1081 taggacaatt cgcatcagct tcgatcgaac gaatgaatcc cttttgttcc caatacctta
     1141 attgacgtgg agacacacct acgatatcac ttaattcact gatgccaaca accaattgat
     1201 cattttccaa caaacgttta agggaagccc caaccatttt tgtcactcct tttgtcctct
     1261 tgcttccctt aattatatcc gtattaactt atgtgtcaag taggttgaca aatagcgaat
     1321 ttaggactaa agtattctag tagtttcaga aaaaattcag aaagaaggag aaatatgagt
     1381 aacaaccaac ccgtagatat ttacggaaaa ccatataatc ggacactttt agttgttgtc
     1441 ttattaattg gtacattttg taccgttttg aatcagacct tattgacgac agcttttcct
     1501 acattgatga aagctttcga tatttcagcg tctgatgtgc aatggttaac gactggtttc
     1561 ttgctagtca acgggatcat gatcccaata acagcatggc tgatcaataa attcagttca
     1621 agaaatttat atctttctgc catgacgatc tttttgatcg gaacgattac ttgctttaca
     1681 gcacctaatt ttagtacatt actaatcggt cgtttgatcc aagcttgcgg tgtgggagta
     1741 tcgatgccat tattgcaaaa tatcatgcta tctattttcc cgccagaaaa aagaggatca
     1801 gcaatgggga tgtcagggat cgttattgga cttgctcctg ctttaggccc tacattatct
     1861 ggttatatca ttgataacta ccattggcgt gacttgtttg gtatggtact gccaatcgtt
     1921 gttcttgtct tggttttagc attcttccta atgaaaagtg ttattccatt gtcaaatcct
     1981 agcattgata ttttatccgc tattttatca acgattggat tcggtagttt actttacgga
     2041 ttttctagtg taggagatga cggctgggga agcttgaaag taattggttt cttagttgtt
     2101 ggtgtgattt tcatcgcctt gttctcatgg cgtcaattac atttggaaca tccattcttg
     2161 gaacttcgtg tattcaaatc atctacattc acgattgcag ctgttttagc tggtgtaaca
     2221 aatatggcga tggtaggggc agaaatggtc gtaccattgt atattcaaaa tattcgtgga
     2281 gaatcagcat tccattctgg cttgatgttg ttgccgggtg cattgatcat gggggctatg
     2341 atgcctgtga cgggtgcgat tttcgataaa tacggcgcga aacgtttagc catcacaggg
     2401 atgttcattt tgacagcggc aactgcacca tttgctttct tgacaaaaac gactccagtt
     2461 ttatatatcg tgatccttta tgcgatccgt atgtttggga tctccatggt catgatgccg
     2521 gtaacgactt ctggtatgaa tgcattgcca aataacttga tcagtcatgg gacagcggta
     2581 aataatacat tccgtcaaat cgcaagctcg attggtacag ctattttgat cagtgtgttg
     2641 acgaatgtga caaaagcaaa tatcccaagc aaatctgtat tggataatac gccattagct
     2701 tataaagatc aggcaatcaa tgccacactg tcaggttacc atgccgcgtt tatcgtagcg
     2761 atcatctttg ggattcttgg ctttattatt gcctttttat tgaagaaaaa agaacctgtt
     2821 ctaatgaaag cgggggataa tgcatgatta ttggagtttt gattgtcagc ttgcttgctt
     2881 ttgctttaac gaacgtgttc gctaaaaaaa catggcaaac ctttttatct ttgattttcg
     2941 gactaatctt cgtcgcttca ctaagtttga tcgtcgctaa tttgtccaat cactttggaa
     3001 tggaaaaagt aacggaaaca aaaacagaaa aaattgtttc cagtgctgat tcccaaggag
     3061 cagatatgct tttgtataaa gctttaggga atggaaaaga aaaagtgtat ctttaccgaa
     3121 caaatgaaaa acaagaaaaa ccaaaagcaa ctggtacgga taacgaaaca aataaagtag
     3181 aaaagacagg cggcgatgct gaaaaagtga cgaaaactac ttattgggaa tacaaaaacg
     3241 acatgtataa attctggttc aatattgctg ataacaatca cgaatatgac agccgagtga
     3301 atacattcaa aatcccagag acatgggtag aattaagtac ggaccaagca gccaagttag
     3361 ctgaattagt gaagaaacag caaagtacga tggaaagtga agcaaaagct tatgttcaag
     3421 acggtatggt aaaagcaatg actgaaaatc caaaaatgag caaagcagag caagaacaac
     3481 gtacgaaagc cttagctgct gaattccaac aacaagcatt cgcaaaatta gtgaaagaag
     3541 caaaaggcga ataatatttt gaacaccatg ta
//
LOCUS       DAKBVG010000214         3571 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000214 DAKBVG010000000
VERSION     DAKBVG010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.214"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            21..491
                     /locus_tag="OUD09_002419"
     CDS             21..491
                     /locus_tag="OUD09_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HCT9693650.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            576..1184
                     /locus_tag="OUD09_002420"
     CDS             576..1184
                     /locus_tag="OUD09_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HCT9693651.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNAAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            1318..1518
                     /locus_tag="OUD09_002421"
     CDS             1318..1518
                     /locus_tag="OUD09_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCT9693652.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            2272..3498
                     /locus_tag="OUD09_002422"
     CDS             2272..3498
                     /locus_tag="OUD09_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCT9693653.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
BASE COUNT         1229 a          524 c          737 g         1081 t
ORIGIN      
        1 ttaataaggt aggttggctc atgaagcagt atcgacagtt aaaaggatat ttagaagcaa
       61 aaggatattg ggagatacta gtctatctct tttttggtgg tttggctacg attgtaaatt
      121 ttgttagttt tgctctcgca cgccaatatt ttgatttaag tatggcctta tccaattcta
      181 tttcgtggtt ttgttctgta ttgtttgctt ttgtaacgaa caaactttgg gtattccatt
      241 caaaatcacc gaattttaca catgcactta ttgaatgcgg taagttcttc ttctaccgta
      301 tcctttctta cggattggac atgggggcaa tggtattgtt gatcaatgtt atgaacagca
      361 atgagtatgt ggcgaaaatc atcactcaaa ttattgttat cctagcgaac tatattttca
      421 gcaagttgtt tatttttaaa gaaactgagg tttttgaaga agaagtaggt caaagtgaaa
      481 aagacgacta ggcataacaa ttgaagaatt tccttctctt tgttactctt tgtaatgtag
      541 acattataaa gaaaacattg aggaggaatt tttttatgac ttacacatta ccagatctac
      601 cttatgcata tgacgcattg gaaccttata ttgacgaaga aacgatgcat ctgcatcatg
      661 ataagcatca caatacttat gtgacgaatt taaatgcagc aattgagaaa tacccagaat
      721 taggcgaaaa aacaatagaa gaattattat ctgatatgga cgctattcca acagatatca
      781 agacagctgt acgtaacaat ggtggcggac atgctaacca ttcatttttc tgggaaatca
      841 tggcaccaaa tgctggtggc gaacctacag gagaaataaa agaagcgatt aatgaagctt
      901 ttggtgattt ttcttctttt aaagaagaat tcaaaaaagc agccgctgga cgatttggtt
      961 ctggatgggc ttggcttgta atggaaaatg gaaaattagc tattacctct actgcaaatc
     1021 aagattctcc attgatggaa ggaaagacac caattctagg tttggacgtt tgggaacatg
     1081 cttattattt gaaatataaa aatgtccgac cagattatat tgcagcattc tggaatgtta
     1141 ttaattggga tgaagtaaac aaacgtttcg aagcagcaaa ataaccattt cctccgtcat
     1201 atcagtctta aaaataattt ggaaaataaa agagcgaaag cttgctagcg ttttctaaat
     1261 tgggtataat gttcttgcaa ggttacttgc aagaactttt ttggaggcta tacatttatg
     1321 gaacacggaa cggttaaatg gttcaacaat gaaaaaggat ttgggtttat tacagtagac
     1381 ggcggagatg acgtatttgt tcatttctca gcaattcaag gagacggctt caagagccta
     1441 gaagaaggtc aagaagtaga attcacgatt gttgaaggtg cacgcggacc tcaagctgct
     1501 gaagtgacta aattataata atttgttgaa taaagacacg acttcttaga gagtcgtgtc
     1561 tttttgtcta tttatgaagt ttttagattt tttggctaag taaatgcatt tgttcacttg
     1621 acatgtccta actaattatc aagcaaggaa cattttcttc gttagtaatg gattttaaag
     1681 aaatttcagt ttttggatgt tgaataaaag accatttgtg aaaccactag tatacatact
     1741 cattaccatg tcaattctat tactttaaaa gaaaatcgtt ttactttgaa gaggagcata
     1801 tctatccatt aaaagatgat ggtaatgaaa tcactagttc gagtcatttt aagagccatg
     1861 cagtgagctg gaaataaaac gaatagaaga tgggtttaac aaaaggacca gtcataggaa
     1921 gaaattttcg tgaaatcgaa tgataatctg aacggtaggc aaagactgtt aaaaagatgg
     1981 gaaaggtatg tgcgatcatt ttactgcacg attatttgga aaataccttg ttgttcgctt
     2041 tttctggaaa aaatacgtaa aaaaagataa aacgattgcg aactgcgtgt attcggtctt
     2101 tcgatctttt tcacggtatg gtatgatagt taagaatttt gagaaatttt aaaaaatagt
     2161 aatcgttaca caaaaatgtt ttttaaagtt ttcttatgat taaaaagtga atttgctgat
     2221 tgaacagtta tatgagacaa tattttagaa aatgtgaaac agaggtgtat tatgaagcga
     2281 tggcaaaaag ttctattaac cttgttaggg gtatttgttt tgctgattgc cggattatca
     2341 gcatacggaa tcagatttat gggagaagcg agccaaacag taaacaaaat ttctaaaaac
     2401 tctaatcgaa tatcctccaa acgttctgag agagtaagca ttgatgacaa agaacctttt
     2461 tcggttttgc tattaggttt agataccggt ggattaggaa gaacggaaca aggacgttct
     2521 gatacgatga tggtagtaac agttaatccg aaacaaaaaa aatcaacgat catcagtttg
     2581 gaccgagata tttatacgaa tatcgtaggt tatggaacgg tagataaatt aaatcatgcc
     2641 tatgcatttg gtggtgtcga aatggccatg gattcaattg agcaactgct cgatattcct
     2701 attgatcatt atgtaacgat caatttggat ggcatggaag atttgatcaa tgcggtcggt
     2761 ggcatcaaag tgaataataa aattgacttt actttggatg gtgtccatgt accagtaggt
     2821 gaacaaacat tgaatggaga aaaaggttta gcctattctc gaatgcgaca cgaagatcca
     2881 gaaggcgata tcggtcgaca acgtcgtcag cgggaagtag tgacgaaaat cgtcaataaa
     2941 gttttaagtt tagatggcgt aagcaattat agaaaaattt taaaagcagt tgagaacaat
     3001 gtgacgacgg acttagattg ggatgacatg cttgatgtag cgacgaacta cacccctgcg
     3061 tttgaaacaa tcaaacaaga ccagttgcaa ggcgaaggac aaatgatcaa tgatatctac
     3121 tatcaaattt taggaaaaaa tgatcttctt tcgatccaaa atgaactgaa gaaacaatta
     3181 aatataaaaa caagcgatac attacctaat ttgaaaaatg agaatgcttc aatgatgttc
     3241 tttgatgatt cggaagatgg cgattcgaca aacggtgatg gatcgaatag tgaagggtac
     3301 accgattatt cttcctattc agaaaacaca tatgatcagt accaagatcc gaatacctat
     3361 tatcagcaaa cagaagatac aagtcagtac tatacaccaa gtcaagatac tcaaagttat
     3421 gatccttacg ctggacagga acaaaatggt tatggttatg accagaataa tgagtatgga
     3481 acaacaagtg ggtactagaa aaaaatagtt tggttaataa agagtgccac ctaaattaga
     3541 caaaaaatct aacttaggtg gcactttttc t
//
LOCUS       DAKBVG010000215         3545 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000215 DAKBVG010000000
VERSION     DAKBVG010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3545
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.215"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(<1..808)
                     /locus_tag="OUD09_002423"
     CDS             complement(<1..808)
                     /locus_tag="OUD09_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCT9693654.1"
                     /translation="MRRLSEMKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNM
                     VAPTKEFVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKG
                     QNLYKPLFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAV
                     IAGITAWKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYL
                     LVMTIVQGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAI"
     gene            complement(875..2164)
                     /locus_tag="OUD09_002424"
     CDS             complement(875..2164)
                     /locus_tag="OUD09_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCT9693655.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(2392..3036)
                     /locus_tag="OUD09_002425"
                     /pseudo
     CDS             complement(2392..3036)
                     /locus_tag="OUD09_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
BASE COUNT         1039 a          829 c          526 g         1151 t
ORIGIN      
        1 aaattgctga agattctccg actttataca tttcaaaagc cttttgataa gcataataga
       61 tcacgtttgc actaccatta ttcggtccac cacgagtgat gatcttaatc ggagtaaaca
      121 catactgcag accttgaaca atggtcatca ccaataaata tagtcctgtc gaactactca
      181 taggcaacac aattgtaaaa aaaatgcgcc agatgggtac acgatccaac ttagctgctt
      241 cgatcatctc ttgatcaatt cccacaattg ctgcaaaaat gacaatgaaa ttataaccaa
      301 aaactttcca agcagtaata cctgcaatca cagcaattac ccaaccttgt aaattcatcc
      361 acataggcag tcttaaacca aaatttccca acaagacagc aattggtccc gataccggat
      421 ttaatatcca tgtaaaaacc atccccgcca ctaccaaaga aatcacagct ggaataaaaa
      481 ataatggttt atacaaattc tgcccttttt ctataactat tcctaataaa aaggatgtaa
      541 tatatgggaa cacacagctc agcaagacaa atagaacggt ataggacaaa gtattcttca
      601 acactttatg aaagactgga tcagtcagca cctttacata gttggcaaag ccaacaaatt
      661 ctttagtggg ggccaccata ttccagtcaa aaaaactcaa atagatcgta taaatcaatg
      721 gataaaaaac aaacacgccc aacaaaataa ttgctggcgc cagatacaaa taacctacat
      781 agcgcttcat ttccgacagc ctcctcatcc ttatgtcaaa agcagacgac cttgtttttc
      841 ctttcttaca ggcgctgtcg tctgcttgct ttgcttattt gagtaactca ttaatttcct
      901 tttgagtagc tttcatcgtt tcattgacgg aattacctga taaaatttta tcccgcatat
      961 ccaacaattt ttgttcagct tctaatccag catctccagg aaatgaactc caactgacca
     1021 tcttatccat ctgatcaatt gctggtatca tcatctgatt ttcagctaag aaagctttta
     1081 atccattttg ggcgtctgca acatctttac gtggtggtaa ataaccagta ccttctgtcc
     1141 atttagctaa agattcaaca ctataaagat attttaagaa atcccaagtt gcttcctttt
     1201 tcttatccgt ttgtgatgta acagctaaca ttgcgccacc agcaggtagt ttaacttttt
     1261 tcccttccca acttggtgac tcaactgctt tagcagtaaa agtactattt ttttgaatgt
     1321 tgtccctttg agcaatagtg gtatagacca tcgcgacgtt accatcaata aagctttgca
     1381 caccttgttc ccacgtagtg tgtaatgccg tctggtcttt taccatcctt tgatataatt
     1441 cataagcagc gataccatct ttagaagcaa aggtagcttt gccatctttt attacacttg
     1501 ccccattact ttctaaaatt gcttgttgtg cccaattatc agcaggttct tgaatataca
     1561 cgccatactt tccagttttt tcttttatta tcttagaaaa agtttgaact tcttcccaag
     1621 tttttggacc attttcatcc aagcccgctg cttttaatat atcttgattt aaatataaaa
     1681 ccggattact aatggagtaa ggaataccca cttgtttacc atctttttca gacagtgcca
     1741 tgatattgtc taaaaaatga tctttaaaat aatcagcatt atccggatcc acttcttcta
     1801 ctaaagattg aggattagta tatttgaaat tttctgcaaa ataatcttta aaagcccatc
     1861 ccacttgtac tacgtccggt atttctttgg aggcaacaga agcttgtaaa ttttgcatca
     1921 agcctttata catatcagga ttatattttg ctacaacctt cactttgtct tgagattgat
     1981 tgtatttatc tactaattca ttcactgttt ttccacctag ggtatccgca ttaacatgcc
     2041 aatactctat cgttacacga ccctccgatt tttttgttgt cttggtattc ccactaccac
     2101 aagctgccaa agagatgaca cttaaaacag acacaacccc tataacaaga tttttcttta
     2161 acataacatt aacttcctcc taatttcttt atggccattt ttctaatcaa gaaatattat
     2221 gaactaccaa tgtaaatttc tagttaattt tctgtaaata attcgaaatt tgtgcatttc
     2281 ggggtacagg gtcactggac atatccatca agctaagaat aaagccccca tatggtcatg
     2341 tatcccatct ccaaatctct taactcctct gttttgatta taaaatagtt tattctgtgc
     2401 tcctttcttc cataaatagg cccatgcaca acgtgaggtt atattataaa caccacagcg
     2461 cgcaaggtac tcaatcgttt ctagttcatt gactgtccat acagaaaatg tcaatcctag
     2521 ctgctgcccc acttcacggg ccaacttagt taaccatcct tcatgaatat tgacgatttg
     2581 aacgctttga taggctaatt tctgtaaggt atcgatcaaa tctgcttcgg taatatcccc
     2641 aagttttttc ccttcgaact gattttcaat attataaaat atatgctttc cccaaatcgc
     2701 agcccaagat aattgtacat agccactaag aacaatttgt ggccagattt gataggtttg
     2761 tgccagcgaa aaaacggcca tttccaatcc ctcttctttt aaatcacagt taatggtcat
     2821 ggttgccttc tcattggcca ctgcttgaaa cacctgctca agggttaccg aatctgggac
     2881 aacttcttac ctgtcatggg ctagtattag cgtaccatct gtttgctgac gcacatctac
     2941 ttcaaaacaa agctggcgat ccaaacaaaa gcgaagaaat gcccagctat tctcctccgt
     3001 attgtcacta ccagagtgtg cagtgatcat ggtcattttg acccctcctt taattccgtg
     3061 attttctccc acacgtccac tataccgatg aagcgttaat ttcttgttaa tttcttgtaa
     3121 attccctgcg ttttttcagt aaactagttg taaatcttgc atgctccttc ctacgaactc
     3181 atgaatcgtc gattttgccc ttctagctag tttcaccaat taagtagttc tacattcccc
     3241 gcttttaacc tactacacct ttttttgttt tcagctattc cttcttcttg ctgaaagtcc
     3301 ttgaggttct ttcttacagg ataccatcaa ggtgttcaat ggcttattcc cattgaatga
     3361 ccgagactta ctatcagatt tctgctcgtt gaaccaagtc tttgatacag gatttcttcc
     3421 gtcatcttct cacaaatacc accttgcgtt tctctagcga ttggtcgatg tgcaccctcc
     3481 cagtggactg tcactacccc tttttgctat gcctagccca caaaaaaagc tgtcctcaca
     3541 gacag
//
LOCUS       DAKBVG010000216         3522 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000216 DAKBVG010000000
VERSION     DAKBVG010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.216"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            27..251
                     /locus_tag="OUD09_002426"
     CDS             27..251
                     /locus_tag="OUD09_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HCT9693656.1"
                     /translation="METEWQIRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(456..776)
                     /locus_tag="OUD09_002427"
     CDS             complement(456..776)
                     /locus_tag="OUD09_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HCT9693657.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(924..3158)
                     /locus_tag="OUD09_002428"
     CDS             complement(924..3158)
                     /locus_tag="OUD09_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCT9693658.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
BASE COUNT         1030 a          663 c          663 g         1166 t
ORIGIN      
        1 tgattaaaga aaggagagtg tgacacatgg aaacagaatg gcaaatacgt gaaagaaaaa
       61 aacagaaaaa agaagagatg ttacgattat gtcgattatt agatgtaaaa gactgggaca
      121 aaaacgagca aattgcacaa aaagtacgat caatcatcca taaagaggaa aaagttaaaa
      181 ataaagacac gcgtagaact gaaaatgaaa agagaaaagt acaaaagaaa agagaaaaat
      241 caaaaagcta gaaagaaaaa gaggctgaga cagaagcgtt taactccaag aaataagctg
      301 gaattcacaa aaatttgaaa agcaattttc gtgaatttta gcttatttcc gaaggagttg
      361 cttctgcttc cgccgtttat acgtgtttag agcgtgagac aaaagtgttc tttacttttg
      421 tcccacgctc tttaattatt atgttcaaaa tttgtttaca cggctaatgt agcgcctaat
      481 tcatgttttg gaatcgagat ttcaacaagg cctgccaatt cgattacttt gatttcatcc
      541 tcgtggtaaa cggcgtcttt gattttatct aagaaataat ttttagcatc ttcgatttct
      601 ttcggctgga ttttacgttt gtcattgatc agcagaattt cattatgttc aggggcttca
      661 gtgtagacaa tagtggtatt tccggctgtg tagaccttga ctagatgggc atcagtgttt
      721 tcaagctggt ttaatacaag acgggaatgg ctgtttgtta catttaccaa tttcatggcg
      781 ctcacctctt ttatgtattt ttaattgctt gtttctctta ttataatttc ttttttataa
      841 aatgaacagt attttgactt ttctgaaaaa agaatgagac ggcatttctc aaagaaatac
      901 cgtctcacgc tataaaactg gatttattca gccgtttctt cggctgattc tttagccaaa
      961 cgttctggtt tagaagtaac gatgattttt ccatctttat ccagttttgc tttcaactca
     1021 tggatagaag ggtgatcaag ataaaattca gcgataccat cttcgatttg ttcttggatc
     1081 gtgcgtcgaa gaggacgagc acccatagat ggatcatagc ctaagtcaac taatttttct
     1141 ttgacattcg taggaacttc gatgtgaagt tgctgtgctg ccagcatttg gttgacatcg
     1201 tctagcatta gtgtgacgat tgtcatcaag ttttcttttg ataatgcact gaattcgatg
     1261 atcccgtcaa aacggttcag aaattctggt gtaaagtagt tgtttaattg gttcaataca
     1321 gaacgagtaa caccttcgcg tgccgcacca aaaccaacgt ttgcttctac tttaccagtt
     1381 ccggcattac ttgtcataat gatgatcgta tctttaaagc tgaccgttct tccttgtgca
     1441 tctgtcaatc gaccatcatc taagatttgc aagaacatat gcaaaacatc tggatgagct
     1501 ttttctactt catctaacaa gacaagactg taaggatttc tccgtacttt ttctgttaat
     1561 tgaccagctt cttcgtatcc gacataacct ggaggagaac caatcaattt agagacacta
     1621 tgtttttcca tatattcaga catatcgaaa cgaatcattg aatcttgtga accgaatagt
     1681 tcgtaggcaa gttgtttcgc aagctctgtt ttacctacac cagttggtcc aacaaataag
     1741 aaggagccga ttggacgatt ctgtttgttt aatccaacac ggtttcgacg aatcgctttc
     1801 gctactcgat ctacagcgtt atcttgtccg atgacatgag ctttcaagtc atctgctaag
     1861 tttttcagtt gtgtctgttc tttttctttt aattcaccca ctggaatacc tgtccgttgt
     1921 tcaacgattt tttccatatc tttttcggta atcacaggtg tttcctcttc agtgagctga
     1981 cgttctttca ttttttgaag tttattgatc tgatcgcgat aataagctgc tttttcgaaa
     2041 tcttcttctc gagaagctaa aactttttgt tcttctgctt cttgcagttt tttctcgatt
     2101 gttttaggat caacgatttg gatcgtcaag tttttcttag aacctgtttc atctagcaaa
     2161 tcgatggctt tatccggcaa gaagcgatct tggacgtaac gattggataa agttgcggct
     2221 gcttcgatgg cctcatcagt atattttaca tgatgatagt cttcataacg tttttgcaaa
     2281 cctttcaaaa tactgatcgt ttcttcgaca gttggttcat ctacccgtac aggttgcata
     2341 cgacgttcta aagcagcgtc tttttcgatg atgcggtact catttaaggt agtagcacca
     2401 accatttgca attctcctcg agctaaagct ggttttaaaa tattaccagc atccatattt
     2461 ccatcaccag cagaaccagc accgacgatt tcgtgaactt catctatgaa taggatcaca
     2521 ttttctgctt gacggatctc ttcaatcaat ttttgcatac gttcttcaaa ttgtcctcgt
     2581 ataccagtac cttggactaa agatacgaca tctaaacgaa tgacttcttt gtccattaat
     2641 ttttgcggta catcaccatc tacgattttt tgtgctaaac cttcaacaac tgctgtttta
     2701 ccaacacctg gttctccaat caagacagga ttgttcttgg ttcggcgatt taaaatttca
     2761 atgactcgtt tgatttcgtc atcgcgtccg atcacaggat cgatatcacc ttgtcgtgct
     2821 tgttctgtga tattgattcc gtattctccc aataaacctt ctgtattgcc ttgaggtggc
     2881 tgaccaccat tgaagttgtt gtttccgcca aattgtgttg gtggtgtttg ctcataattt
     2941 ccttgctgtt gcatttggcg agacatggaa cggaaaagat cgtccaaact gccaaatcca
     3001 aatggatcat tttgggccat attagtcaga cctccatttt gttgattttt gattttttga
     3061 taacagctct gacagtaatc gagttgtgtc cgctgaccat tgacagtggc atacaagtga
     3121 atcgttgcct cattcttgcc gcagttttga caaagcatga atcattcacg tcctttcaca
     3181 aaaatgggta catccatatt ttacatctcg tttttcatga ggtaaaagaa taacccctag
     3241 ttttttaaga gatttatgag gtagaaaagg tgaaatctct attgaccatt cctgaccttt
     3301 gactcttatt atactaaaaa taaaaaggga agcaagtaat tcgtttcata tgaaaacaaa
     3361 gagggtcaaa ggaaagaatg ataaacgtca ggagaagaga aagcgacaag aaaaagaaaa
     3421 taaacacttt tgttataaaa tgtgaagaaa aggatgaaaa agaatccgga ataatttgaa
     3481 tacttttatc tgaaattgaa tgaaaactgt tgtatctaac ga
//
LOCUS       DAKBVG010000217         3520 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000217 DAKBVG010000000
VERSION     DAKBVG010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.217"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(2..331)
                     /locus_tag="OUD09_002429"
     CDS             complement(2..331)
                     /locus_tag="OUD09_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HCT9693659.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            508..1236
                     /gene="sfsA"
                     /locus_tag="OUD09_002430"
     CDS             508..1236
                     /gene="sfsA"
                     /locus_tag="OUD09_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HCT9693660.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            1252..2265
                     /locus_tag="OUD09_002431"
     CDS             1252..2265
                     /locus_tag="OUD09_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCT9693661.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            2265..>3520
                     /locus_tag="OUD09_002432"
     CDS             2265..>3520
                     /locus_tag="OUD09_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCT9693662.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKER"
BASE COUNT         1195 a          584 c          738 g         1003 t
ORIGIN      
        1 tctatttttc agataacaac ttatatgaag tgtctgcaga ttcgtcataa agagtttgtt
       61 cgggataaga aaagataaga taaaaaccat actgatcacg tacgactaaa cgatattcat
      121 ctatttctac cacagatgtg ccaagagact gaccgttgat caaaaacgtg taatcagatg
      181 taatctgtaa ttgttgtttt tgctggctat cactaaagaa ccatgtacct tgatactttg
      241 agagtccttc tgacttttcc gcttgttttt tctttttcaa gtaaataaaa cttgagagaa
      301 cggtaatgag tgaaccaaga aaatagatca ttttttttat caccaaatca ctcctttatg
      361 agaaaataat cgcatagaca tattctttct tctttatttc attgtaccga ataaaagcta
      421 aaatttctct aagactttcg actggttttt ttactgtttt tctacaaatt atgataggat
      481 acatagtagg aagaaaggtg acataatttg aagtatcagc aagtggaaat agcttttttt
      541 attgaccgcc cgaatcggtt tatagcacat tgtatgaaaa aagacggaga aataattatt
      601 gcgcatgtca aaaatacagg tagaggaaaa gaggtcctga taccaggcgc tgaagtggct
      661 attgtatttg ctcctggacc taaacggaaa accgcctatg atctgattgc tgtaaaaaag
      721 aagggtgact ggtttaatat cgatagtcaa ttgcctaatc ggctggcaat tgatggtatt
      781 ttagacggca cgatcctttt accaggatta actggtccat ttacggtata caagcgtgaa
      841 gtaacttatc ttcactccaa atttgatatt tatttagaga cggtggtcgg acaaaaagca
      901 tttgttgaag tcaaaggtat gacacttgaa aataaagcga ttggtgcttt tcctgatgcg
      961 cccacactca gaggattgaa acatatagga gaattaacgt atgccgcaca agatggttat
     1021 gccgcttatg tcttgtttat tgctcagttc gaacatcttc atctggcaac gatccatgaa
     1081 gaaatgcaac cagcactagc tgatatggtt cgacatgcgc agcaaagcgg agttcaaatc
     1141 ctcgcctata attgtcaagt atcggcagat gaggtaactg tcaagcaggc cattccattt
     1201 gatgtagata tcccttttga agatcctata caatagaaag aaggtaaaat aatgatcaac
     1261 ttaggtatta tcggaacaaa ttggattact catcaatttg tccaagcagc tttaagcaca
     1321 aaaaaatatg aattgacagc ggtatattct agacatttgg aaaaagcaca ggaatttggt
     1381 tctcattatg aaggggatat cgaattcgca acagatttga agactttttt caatattact
     1441 catatggaca ccatttatat tgcttctccg aatagtcttc actttagtca agctaagcaa
     1501 gcaattttgg caggaaaaaa tgtgatagtt gaaaagccag ctttttcgac gccaaaagaa
     1561 atggatgaaa tcatccattt ggcaaatgaa cacaatgtgt atttttttga agcagcaaga
     1621 aatatccatg aatcaggttt taaaaagatt gccgattact taccattaaa agacgagata
     1681 atcggtgcaa atttcagcta tatgaaatat tcttcgcgct atgaccaagt attagatgga
     1741 gaagaaccga atatcttctc gactcatttc tcaggtggtg cattgatgga tttaggtgtt
     1801 taccttgtat atgcagctgt tgcttggttt ggaatgccaa aagaagtcca ttatttccca
     1861 agaaagattt ctactggtgt agatggtctt ggatggggga tcttacgtta tgagaatttt
     1921 gatgtatcga tccagccagg taaaatcggt gattcttatt taccgtcaga gatttatttt
     1981 gactcaggaa cgctagtgat gaatggagtc aatgcaattg aacaagcaca atttcatgat
     2041 cgcgcgcata aagaaatcat tgatttggcg gtaacagctg cagaaaatcc aatggcagat
     2101 gaagcagagg attttgccaa tgtcatggct catccgaaag atccagcttg gggattgttg
     2161 tatgaagagt ggatcgagct agctagaaat gtgaaccaag tgatttatga tttaagaaaa
     2221 aatggcggca tcaaattcga tgcagataat gaggaagatt tttaatgaac gaatttgtac
     2281 aagtatttcc cgaaaattgg cagaaaaaat atgaggataa agggtttatc tcaccttctc
     2341 ttattcaacg agcagttttt cagccactaa gtaataaaca aagtatagta gcggtttctc
     2401 ctacaggttc aggtaaaaca ctggcttatc tttggcctct actactaaac gtggaaccag
     2461 gtgaagcaag tgcgttggtc atttttgctt cttctcaaga actagctatt caagtagcag
     2521 atgttgcacg tgaatgggga aaagacaaag agttgaaagt ccaatctttg gtgggcggag
     2581 ccaacgtcaa acgtcagatt gagggtttga aggaaaaacc agaaatcttg attggtacac
     2641 caggaagaat attggaattg atgaaagcaa aaaaaataaa agcacatcaa gtgaaaacaa
     2701 tggtatttga tgaagcagac cagctatttg acgctgggaa cagccaaatc attgatcaaa
     2761 ttcttcatca agctccaaca gagtatcagc tagctttttt ttcagcgaca gcagatcgtt
     2821 cccttgaaag cattgaaaaa atcactggga aaacgtttga gacaattgac gtgacagcag
     2881 aggacgattc tcgaaaagga ctgagacatt actttttacg tgtaccgata cgaaagaaag
     2941 atgactactt gagacgtctc acgcatatag aagattttca tggtctgatt ttctttaatc
     3001 aactgactga attaggcgta atggaagaaa aattgctgta tagaggtgta ccggtaggaa
     3061 gtctggcctc tgatcagaac aagctactcc gaaaagcagc gctagaacaa ttcaaaaaga
     3121 aaatggtcag agcacttttg accacagatg tggcggctag aggtttagat attacaggtc
     3181 ttccttatgt agtgaatgca gaagttccgt taagtgaaga agcttatttg catcgttcgg
     3241 gaagaactgg tcgaatgggt aatgaaggaa tagtaattac actcgtacaa gataataatc
     3301 taagagattt aaaacggctt ttacgtccat caaatataac acttgaagag atttttttat
     3361 acggaggtaa gctgcaaact gagcagccaa ccaaagaaga tgcagctcct gcagtaaata
     3421 aaaagtatgc gaaaactttt tctggaaagg agcagaaccc agaatataac aaagaaaaaa
     3481 taagaaaaaa agttaaaaat aaatcaaaaa aagagagaaa
//
LOCUS       DAKBVG010000218         3498 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000218 DAKBVG010000000
VERSION     DAKBVG010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3498
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.218"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(52..1518)
                     /locus_tag="OUD09_002433"
     CDS             complement(52..1518)
                     /locus_tag="OUD09_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HCT9693663.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1905..2501
                     /locus_tag="OUD09_002434"
     CDS             1905..2501
                     /locus_tag="OUD09_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HCT9693664.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(2559..3041)
                     /locus_tag="OUD09_002435"
     CDS             complement(2559..3041)
                     /locus_tag="OUD09_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693665.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3147..3344)
                     /locus_tag="OUD09_002436"
     CDS             complement(3147..3344)
                     /locus_tag="OUD09_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693666.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
BASE COUNT         1184 a          704 c          576 g         1034 t
ORIGIN      
        1 aaaaaacagg aactgctgca aataagcaat tcctgttttt atgttcattg attaatgaat
       61 agagcccatt agttttttga ttggttcttt cacagtaaat agcaagatac caccgacgat
      121 tgctactgca ccgataatac cgaagtaagc agattctgtt cccggtgtgt agaagcgtgc
      181 aatttgtgca ttgattgctt gtgaagaagc atcagctaag aaccaaatag cgattgtttg
      241 tgattcaaat gctcgtggtg ctaattttgt tgtcacagac aaaccaactg gtgaaagaca
      301 caattcagca ataaccacga tgaagaaact tcccaacaac cacaatggac ttacttttcc
      361 acttgtacca taaagcatac ccggcaacat caatagcaag tacgataatc ctgcaaataa
      421 aagtccaaga gagaatttca ctactgtaga tggttgacgt tcaccaagtt tcgtccacat
      481 cgttacgaat actggtgtca gaataaagat gaaaattgga ttcaatgatt gataccagct
      541 tgcttggatc gttacaccaa atagcgtaga ttgtgtacgt tcactagcaa ataaagctaa
      601 gatagatgaa ccttgttctt gtaatgacca gaaaatgatt gctgcaagga ataatgggat
      661 ataagcccat actcttggtt tttcttcagc agttacatct tttgaagtca gcattttgat
      721 aaagtaatac aacggtaaga taatccccaa tacactgatt gtattgacaa aaaagtcaat
      781 tgtcagatga ccagttagtt gtgccccgcc gaatactgca gcgataacaa caagtgcgat
      841 gatcaagtta cggatgaatc ctttacgttc ttcagttgtc aaaggattcg gcggtgttgt
      901 acccactccg tctaatgtct tgcgtccttg gatgatgtat tgtaataatc caaagaacat
      961 cccaatagca gctaaagaga accctaaatg atagttatat tcttgaccga ttgtaccaac
     1021 gattagtggt gctaataatg cccctaagtt gacacccatg taaaaaatcg aaaatcctgc
     1081 atctcttcgc aagtcagtct ttgaataaag atgtccgacc atgccagaaa catttggttt
     1141 caaaaaggcg gttccgataa taatcaatgc catcgaaaca aacaatgtac ttacaccaaa
     1201 tggtaaagcc aagacgatgt ggccaagcat aatgaaaact ccaccgaaga atacagtgcg
     1261 ttttgctcct aacactcggt cagataccca tccgccgacg atactagaca tgtagatcaa
     1321 agatccgtag atcgacatga tagctaatgc tgttgctcgt ggtaatccaa gtcccccgtt
     1381 ggcaactgta tcgtacatat aatagataag taatgcacgc ataccgtaat aactaaatcg
     1441 ttcccacatc tcagtgaaga acaaagttga caatcccttc ggctgcccga agaagccctt
     1501 gtcttgctgt ttttccataa tctatacccc aattctttaa attttcagaa aatattatac
     1561 atctttaccg aaaaatttaa ctaaaaaact tatttaagaa atataaacta attttctata
     1621 aaagcaagta atttttaatg gcaatatcat aaaaaaaaga agatagagat ttaataatta
     1681 tatatcagct gtttttttca aataagcaaa aattcctaaa acacaacaat gaaagcgata
     1741 actaagaaaa tccttacata acgatttttc ttattaaaat tgttttttga acttcgctta
     1801 ttttttgtga gcatatctgt ttacttactt tttgtaaggt gctatacttc atttcgtaat
     1861 caattaaaaa aatgcaaaac gcaaatcaaa ggagaaattt tattatgaca acttttactg
     1921 acactttgaa aaaccgccgt tcaatctatc atctaggacg taatgtttca ctttctaatg
     1981 aagaattaac aactttgatc aaagaagcaa ttaaagaaag cccaactgct ttcaatgctc
     2041 aatcaacacg cgcagttatc cttttcggag atgcacatga aaaactttgg gaaatcactg
     2101 aagaagcact tcgcccattg actccagcag aagctttccc aaatacacaa aacaaattag
     2161 ctggtttcaa aaatggttat ggtacagtat tattcttcaa agatactgac gtggtaaaag
     2221 gtctacaaga acaatttgaa ttatatgcag acaacttccc agattggtca gaacaatcaa
     2281 acggaatcgc aacagctaat acttgggtag cattagttga taaaggattg ggcgcaaact
     2341 tgcaacatta caacccagtt attgacgaag cagttgcaaa agaatggaat atcccttcaa
     2401 actggaaact acgttcgcaa ttagtattcg gttcaccaga aacaccggct ggggaaaaag
     2461 aatacatgaa cgatgccgat cgcttccgcg tatttggata attttatttg aaataaaaaa
     2521 cagaggttgt gacaaacatc ttgtcgcaac ctctgttttt attctggatc aatcataata
     2581 tcaatctctg cctcatcgtt taagcgttgg gacaaaaatg gaactaattc ttctaatgat
     2641 gcagaaaatt tcagcatcgg tcctcgccat ccgtttcgga aaaaattcat ttttacgatt
     2701 ttagaacctt ttggctgata taatacaatt ttattgatct cagaaaaagg aacacctttt
     2761 ttgtcaaagc tattcaaaac aagtccttct tctgtgattc ctctactgtc caacaaaaaa
     2821 ctaataaaaa tcattgctga caagatccct tttgccaatt catctgctgt aggatgactg
     2881 atgagtgtat accaaatgac tagtgacact aagaagggta tcaccaagta gacccatcga
     2941 tttcgcacag tccgataaat aatttctttt cgaaccagcc aaagaaataa aaacatcaca
     3001 acaacgaata aaaacaatag aaccgtaaag aatgttacca tgacaactcg cctcgctttt
     3061 tcttctccag aaaataggaa aagaatactg atttaccttc tcaaagaaat tggacttgca
     3121 gcacgccgca cgtccaattg ttttctttat tcaaatgtat catattttgc aacaaccatg
     3181 cttgcttttt catctaataa ttcttggtcc tctgcagcac attgggcaac tttgaccacg
     3241 gcgcagtttg tcatttttga tacaacttca ccaatgaccg tttttgtaaa accaattggt
     3301 ttgcatgcat attgttctcc taaattaatc atttcttccg tcatcaaaat cctcctaaac
     3361 tgctggaact tttctccaca ttaccactgc tatttctttg gagatttaac tcattgattt
     3421 ttatttacag ttaaaaatca accttacagg cattatagcg tactgttttc gttttcacaa
     3481 ttaaaacagc tgttgtat
//
LOCUS       DAKBVG010000219         3485 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000219 DAKBVG010000000
VERSION     DAKBVG010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.219"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(<1..2235)
                     /locus_tag="OUD09_002437"
     CDS             complement(<1..2235)
                     /locus_tag="OUD09_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693667.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTGL"
     gene            complement(2907..3175)
                     /locus_tag="OUD09_002438"
                     /pseudo
     CDS             complement(2907..3175)
                     /locus_tag="OUD09_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          980 a          770 c          549 g         1186 t
ORIGIN      
        1 taaaccggtt tgtagcgttt ctccttctcg gttctgtaac tcaaaaacag ctccttgaag
       61 gacttcacca ctgccttcag catgtttaat tagttcaaca ccacccgtat caaggatgtt
      121 ttcaatggtt aacgtcactt cttctatgga gtattcattt ataaaaacat catgtaccgt
      181 cgcatctaaa agataccctt ccggtgcttc tgtttctcgg acttgatagc tacctacagg
      241 aagcggttca cttaaaaatt ctccgttttc tgccgttata ccttcttgta tcacttgtga
      301 atcgcttgat aaaatttcaa attttgctcc tgccagcctt tggccattct catcagtttt
      361 aattacttta atacgcccat cgattttcct tgttcttaca gtactcgtat tcaatcgatt
      421 gtcactattc acaagcgttg caaagtttaa aatttgatcg ccgaccgcat acgcctctac
      481 attgacctta aaagagaggg taaaacttgt cggaccacca atcgtattat tcgcactgaa
      541 acgaactatt ccattattga cttcggcatc aatgaaagag ggaatatctg atagaaatga
      601 cacaaattcc atgccttctt ccaaaacatc ttcaataaca agcgtatttc ttgttccaga
      661 aaacatcgag acatgcagag tatacgttag ttcattttca ttgagattga taacattttt
      721 gtccacactt tttcttaaag agattgccgg aagattactt tcagggattc tcaagcggta
      781 gtctcggctc attacctgat tattgtaggt catactcgct cgattccgag aaaacccatc
      841 ctcttgtctt ccttcattga acaagtcgac atcggtcact tcaagagcat attcaataaa
      901 atacgattga ccctctgcta gattaccaaa gtccacaaca agtgtattcg tttccttatt
      961 aaaagatact ttgtccacaa attcctcgga aacataatga gttctaacgt ttgcttcacc
     1021 tggattcgtt acttctgcga tccgaacccc acctgaaata ggttctctat ttccagtgga
     1081 actccatgta gtcggattcg gtgccaaagt tgttccttgc ggtagtacat cggtaaccac
     1141 tacaccgtct tttcgtgatc cttcataatt tacctgcaaa ataaatttat tactgttggt
     1201 cacgtcttgc ccgtcaatca gcagattccc aagatttcct tggctgtcga tttctctaga
     1261 gccatagtat aatttcgaaa aaatgggtct tgatgaagga ataggcgtaa ttgaaaattc
     1321 cgtgtttgct gctcgctcag cataagtaag actcacttga aagtcattat ttggctcagt
     1381 accgcctgat tgaaagaaag caacttcaaa cgtgatactg aatgtatacg actgagcggc
     1441 ttcatttaat ccttcataat caaaggtaaa tggaaaaaca acgtctgtgc cttcttggta
     1501 aaatgccagt ggctttaaag gtgaatcatc agcgacctcc actctaatga actcaattaa
     1561 gccaatcgat gctctaggaa ctcgtacgac catctcccca tattcttcaa gaatatcgcc
     1621 agcgcctcct tgaatgtatc cctttaacgt ttgtgtatgc ctgctcggca aagtggggcg
     1681 ctcagcagag atgccgatgt cataattcaa gagctcctct tgtctttcaa tctgagattc
     1741 ctgcgttata gatggctcag atgatctggc ttcattaaaa gattcactta agctttcttc
     1801 tggtatatta aaagattcac ttgtgctttc ttctggtaca ttaaaagact cacttgtgct
     1861 ttcttctggt acactgattt ctattgagac cgtatttgat tgaatatcac cgcttcttgc
     1921 ggtcaattcg tatttacctg aatgctggga agaataagta atcagacgtt cgtaatcatt
     1981 gagttgcttc acttcccctt caatcaaatc ctcactctta tcaatgacca atgcatttag
     2041 caggtctggt gttcctctga ctcgcaactg gaactgctca cccaaagcag cctcctcttg
     2101 atcaacttcg atcgtcagcg aacgctctgg ctgttctgct aaagactcag ctagaaccgt
     2161 tatgttccca aattgattga tcacaataag cataactaga aacatactta acagtttatg
     2221 aaatttgttc ctcactatta ctccccctta taattttgac taaacaacaa ggtacccgat
     2281 aattttctaa aaagattccc caactcaaaa tggctgcgtt acctccctcg tattttcggt
     2341 tcaactcaag cgttgcaatg taagctgtaa acaaagctaa tgattgccga ttgtcttctg
     2401 ttgattgatt cgatagcttt gtatgacttt tagttgtccg tgtggcttaa ccataattat
     2461 gatttcacga ttcctcctgc cagtgccttg cacaagctgg agcgtaaccc taatagttat
     2521 tttgttaatg tacttatata cttttatact tttccattag aaacaatcac taaatcgttg
     2581 ataccaaggt cagactctag aatttcatca gtgagtccct gtattggtta accttcatca
     2641 atcccaaaaa aatatatcaa tgcttagcat caaaatgaat atatagttta gaatacttgg
     2701 gattcatcga taactgatcg cttatttggc catattattt ggcacttggt tcaactaaac
     2761 gcatttagga tgttccttca ttctcgtcat ggatgttctc aaaccgtcat tactaataaa
     2821 ctgtccaccg aatcgatggt tcatttcttg cattttccac gtccaattac tctttttgct
     2881 ctttatttgg gcgtagtttc actttcttaa cttgtatcgt cttccatcaa ctccttaatc
     2941 atcttcttag ctttattggc tcggtttcct tgtaaaaggt atctacaaat agtgacaatc
     3001 tgtattaaat cttcatcatt ttctgtaatg tcaataattt ctatattctg taccatatat
     3061 tgcaaagggt ttcactaagt tcataaccga agcgaattaa acaacttttg tatagaataa
     3121 ctaccttttc tatttttcca ttggtaattc tatctattaa ttggtttaat ccttttgttg
     3181 tagttgattc cactaccaag atgattctct cgaattttaa agcttgaaga tgtattgcta
     3241 tttttgctaa ctggcgtatt tgcaactatt tttcccttct attcttcctt cattgattcc
     3301 cctctaaaat tactatccat aaaaaattgc ataaatgaca atagtattta tattagtcat
     3361 actgcttttt aacacagcaa attccagaaa aacattcgtt acatatttgt gaatttttcc
     3421 aaaaaataac atttttataa aacgacgaaa aataaatcac taccagtcaa cttctataat
     3481 attac
//
LOCUS       DAKBVG010000220         3443 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000220 DAKBVG010000000
VERSION     DAKBVG010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3443
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.221"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(<1..1157)
                     /locus_tag="OUD09_002439"
     CDS             complement(<1..1157)
                     /locus_tag="OUD09_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCT9693668.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTRV"
     gene            complement(1160..3409)
                     /locus_tag="OUD09_002440"
     CDS             complement(1160..3409)
                     /locus_tag="OUD09_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCT9693669.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
BASE COUNT         1117 a          646 c          493 g         1187 t
ORIGIN      
        1 actctagtat tttttttatt tgtcgcaaaa aatgttacaa ataggatcac aataaatatt
       61 cctgcgaata caaaaaatgg agcagtcaaa tttgtacttc ttgttagaaa ttgtgctaat
      121 tgcatagcaa taggagctat aaaattacca atattacagc caataaaaat caatgaagta
      181 gcaaaattaa ttgtattctt tgtagtgact ttatctaagt tactaaagat aaatgggaag
      241 caccatgatc ctgttatact tgataaaaat aaacccgtta gcaggaacgt catattaccg
      301 tttgacacag caattaacaa atttgaaatt ataaagaata taattcccaa gtatagggtt
      361 ccttttccta tccacttatt tattggtcca aacataaatc ctgctagtat tcctaagata
      421 ggcattagag ctaaatagtt actagcatta aagtttaccc ctttaatttc agttgcaatt
      481 gatgcaaatc gtacagaaat agcaatacta ttcattacga gtaagattgc aaatagaact
      541 agggcaaaaa ccattggatt catcttttct tttacatgat tttcaggtat gtcactattt
      601 tcatctctaa tttcaggaac ttttagcgcg aataagagag ctacaggaaa cgcaattgca
      661 tagactaaaa acgaagccgt ccaattaatg ttcactaaaa ctccagcaag aaaaataagg
      721 attgtctgac ctaaagcttc cattgaattt ctaataccta atagcgttgc tctagtattg
      781 ccagagtata aagagctgat atatgtcaca gccaatgaat tatatactcc taaaccggca
      841 cctaatatta ctcgactaat aagaacggtt tggaaggtag ctgtaaatac tggaataatt
      901 cctcctatac cagctaataa taaacctaaa attattgtct ttttcattcc tattcttttg
      961 gcaaagtaac tagataataa gataaacaaa ataacagtta tacttggtgc agttcctaaa
     1021 agttcacttt gactttgtga tatccccaaa gactgtttca tttgtggaat aacaccatta
     1081 attgcttgag agcttgttaa cattagtgaa atacttaaga ttgctccttt ttgaagtaag
     1141 cttttttggt tattcatctc tacttttcct cctgtgttat aacccaaatc ttggaatcaa
     1201 aatcaaatgt ttctgggcgt cgattctgat catttatttg tccactagat gcatctgaac
     1261 agactagtcc tgctctcatc aattccattc cagatcgcaa actattttgt cctacattat
     1321 aaagtccgtc attatctaac ccttttaatt tcagtcggcg gtaaggagca tttacctcat
     1381 ttaatgtttt ataatgagca acaatagccg ttttcttatc atgactaaca accatccacg
     1441 cggtttgatt atcatcatcg aatggtgata atagtctata aaaagtacca ttatgaatta
     1501 tggttctata ttttttataa aaggcaattt gttcttttac aatcctcaaa tcgccttcac
     1561 ttaattcatt taagtcaagc tcatatccaa atgcaccaaa aaaagctaca tttcctctag
     1621 tttttatagg tgttatacgg tttgtttgat gatttggaac aattgataca tgagcaccaa
     1681 tagacgaaag aggatacagc atactagttc catattgaat ttttaacctt tcaattgcat
     1741 ctgaatcatc actagcccaa gcttgaggtg catagtgaag cattccagga tcaaagcgcc
     1801 caccacctga tgaacaagac tcaaatagta tgttaggata ttttgttata agtttttcat
     1861 ataagcgata tacgcctagt acatatctgt ggaatacttc tccttgttga tctgctgaca
     1921 agtgactgtc aaatacatca gtcaatggac gattcatatc ccacttaatg taatctaagt
     1981 ttgattcatc aatgatttta cacatttgat cgaaaatatt ttcaacaacc tcatctaacc
     2041 caaaatttaa tacatattgg ttcctaccat gattaggttt tcgattaggt gtagaaataa
     2101 tccaattcgg atgtgtgcga tataactcac tatccttatt caccatttca ggttcaaacc
     2161 ataaaccaaa tttcatacca aaactacgaa ttttttctga aaggctgccg attcctctag
     2221 gtagtctttt aggatttaca tgccaatctc ctaatcctgc acaatcgttt tctctttctc
     2281 caaaccagcc atcatctaat acaaataatt caataccaac atcaaatgct tttttagcaa
     2341 tatttaccaa cttttcttcg tcaaaatcaa aatatgttgc ttcccaatta ttaattaaga
     2401 caggacggtc tttttctcgc cacttaccac gagctaagcg ttttctaaat agttgatgaa
     2461 aagtttgact catggcattt aaacccttac ttgaataaac cagaatcgct tctggtgctg
     2521 taaatatttc tcctggattg agtttccaat taaatccaaa tggattgatg ccaacttgta
     2581 gtctagtaac atcccatgta tctacttctg cttgaattaa gaagtttcct gagtaaacta
     2641 gtgctgcacc aatagcttct ccttgatttt catcagtatt ttttcttttt aaagtaacaa
     2701 atgggttatg ctgatggctt gaaatacctc gagtagattc gattgattga attccttgtt
     2761 gtaaagtacg cgacttaata gttctttctc taccccaagc accagataat tgttgccatt
     2821 cataatttgc atcgggtaaa tccaatgata aactgctaat attctcgatt atttgaatat
     2881 cttttccttt attcataact cgcgcgctac gagtaataac aggataatct ctataaattg
     2941 tatataataa ttctacttga acatttgtaa ctgaatctaa aatataaact ttcaatgttt
     3001 tgctttcatc attattatca acataggtag caggtaaacc ttctagttta ggttttcctt
     3061 cttccacttc gtacccttga tagacaaaat ctgataagcg agagccattt ttctgtagta
     3121 atgaaatagc aggatgtcta aaatctgttg taccatattc aggaaattct tgttttaagt
     3181 gctctaacga atatagcaaa tcgttttcat ttctataggt tgtcataggc cgatgatgca
     3241 tctcaattag atgactatag tcattatctg gtaagttctc accataatat aaatgcaata
     3301 atttaccatc ttccgatatc ttaaaaatgt aacttatctc ctgattaaac aaatgaaatt
     3361 gttggttttc tgatcgataa ataatattca tttcattatc cttatacata agctctcctc
     3421 cgtttcttta ttgcattata aaa
//
LOCUS       DAKBVG010000221         3366 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000221 DAKBVG010000000
VERSION     DAKBVG010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.223"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            236..1627
                     /locus_tag="OUD09_002441"
     CDS             236..1627
                     /locus_tag="OUD09_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HCT9693670.1"
                     /translation="MNKNGEWNAARRIFIILMDVLVLNVSIYLSFLLKFQGEVPVRNL
                     ETFQHSAIFISIIFIALNILLGAYVFYNRMISDIVFITVIGQVLIALGIMVVTFAGRW
                     FAFPRGVILISFILGTILLSVYRIVVYKLYLRVSRDKKVLIVGLEKDVAPAIQNFQSK
                     KNNKHKVEAVAIDNYYENTKKLIDDMDIVYLASKIPEDEKINIYQLVTKKEKKLFLNT
                     TFENLTMINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLILLILASPFMLIT
                     AILVKTTSPGPIIYKQVRITKNQKEFSIYKFRSMSATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNEENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLLLDLKILLQTIKILFDKMSSQGLDEDENISS
                     LAESAFPKEKTYR"
     gene            1665..2495
                     /locus_tag="OUD09_002442"
     CDS             1665..2495
                     /locus_tag="OUD09_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCT9693671.1"
                     /translation="MENKNISILLSLYDKEKPEYLKEALASIFEQTVMPAEIVLVYDG
                     PINEKLQAVVTYFQQKVPHLFTVVKLEKNQGLGIALATGLEHTKNRLVARMDTDDIME
                     PHRLEKQLEIFQEYPEIAIVGSNIEEFTGTLSNVIGRRIVPEYNDEIRVFSKRRNPFN
                     HMTVMFDKNAILEVGNYRPLIGFEDYYLWVRLLKAGYKGYNIQEILVHARAGADMYAR
                     RGGSKYLLPGIKGRYRVWKDGLGSLKDFLIVICGHLVISLLPNKIRGKVYESKLRKTN
                     "
     gene            2467..>3366
                     /locus_tag="OUD09_002443"
     CDS             2467..>3366
                     /locus_tag="OUD09_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCT9693672.1"
                     /translation="MNQNLEKQTKLSIIIPFYNAKNTLRRAVDSVLTQGYPNIEILLI
                     DDGSTDGSSDIGDEIANLESKVACYHLENAGPSVARNYGIKEATGNLVGFLDSDDYFL
                     PDCFNNVLKDYHRNHPDIIAFGLSKGKNIENANLFVPEKKIGCSSEEAIEQMFRSKAV
                     DFYVWNKFFKRELFNHIVFPENKLYEDMVPMYEAFRAASKIDILPFSGIFYYQNNDSI
                     VYQSFNSKQYDNILQRKILVDEVKKDYPRLINLANARLVDGYLSTGFKITGKNQNQKE
                     KQKYYNQSKREIKSIYSKIRSNNE"
BASE COUNT         1195 a          467 c          648 g         1056 t
ORIGIN      
        1 tatgaatatt gtaacttgtg aataaggaat tagcaggaga atactagttc agcataaagg
       61 aagtgatggg acttctgaga caactttctg ttatgcagaa atacacatat aaacagacaa
      121 taaacgcttt tccccgtttc ttttaatttc ctttacaatt tggttattca ctaggttttt
      181 aaactttgct atggtaagat gatagaggca ttattttatg gttaggagtg tatatatgaa
      241 taaaaatggg gagtggaatg cggcacgcag aatattcatt attcttatgg atgtacttgt
      301 attaaatgta tcgatctatc ttagtttttt gctgaaattc cagggagaag taccagtgag
      361 aaacttagaa acctttcagc attcagcaat ttttatttcc atcatcttta ttgcgttgaa
      421 tattttgtta ggagcttatg tattctataa ccgaatgatc agtgatattg tcttcattac
      481 tgttattgga caagtactaa tagcattggg gatcatggta gttacttttg ctggccgatg
      541 gtttgcattt ccgcgtggcg tcattttgat ctcgtttatc ctaggtacga tattacttag
      601 tgtttaccgg atagtcgttt acaaactgta cttaagagtc agtcgtgata agaaagtcct
      661 tatcgtgggt ttggagaaag atgtcgcacc agctatccaa aattttcaat ctaagaaaaa
      721 caataaacat aaggttgaag cagtggcaat cgataattat tacgaaaata caaaaaaatt
      781 gatcgatgat atggatatcg tctatttggc ttctaaaatc ccagaagatg aaaaaatcaa
      841 catttatcaa ttagtgacta aaaaagagaa gaaattattt ttaaatacga cttttgagaa
      901 tctgacaatg ataaatccga atattatgaa ttttgaagat gaaagcatca ttgaagcttc
      961 tgggttcaga atccaaccag aatacgaact attcaagcgt ctctttgata tcatcgtttc
     1021 gcttatttta ctgatcctcg cctcaccttt tatgctaatc acagcgattt tggtaaaaac
     1081 aacttcacct ggaccaatca tctataaaca agtacgtatt acgaaaaatc aaaaggaatt
     1141 ttcaatctat aaattccgtt ccatgagtgc gacagctgaa gcaaaatcag gaccagtctt
     1201 ggctaaaagt aacgatgcac gtgtgacacc agtaggtaaa tttatccgtg ctgtacgttt
     1261 tgacgaatta cctcaaatct ttaatgtatt gaaaggcgat atgtcgattg tcggtccaag
     1321 gccagaaaga ccatttttcg ttgatcagtt caatgaagaa aatccttatt attacttaag
     1381 acacaatgta cgtgcgggaa ttaccggata tgcacaagta tacggaaaat atgtatcaga
     1441 ttataacagt aaactacgtt ttgatttgtt gtatatcaaa aagtattcct tactattaga
     1501 tttgaagatt ttacttcaaa caatcaaaat cttatttgat aagatgtcct cacaaggact
     1561 agatgaagat gaaaatatca gttcattagc agaaagtgca tttccaaaag aaaaaacgta
     1621 tcgttagatt ggaaaaattt tgagcaaaga agtaggagag aacattggaa aataaaaata
     1681 taagtatact gctttctttg tatgataaag aaaagccgga atatttaaag gaagcattag
     1741 ctagtatctt tgagcaaacg gtgatgccgg cagaaatcgt attggtttat gatggcccta
     1801 taaatgagaa gcttcaggca gttgtcactt atttccagca aaaagttcct cacttgttta
     1861 cagttgtaaa attagaaaaa aatcaaggat tggggattgc tttagcaact ggattagaac
     1921 atactaaaaa tcgcttagtg gccagaatgg atacagatga catcatggaa ccacacagat
     1981 tggaaaaaca attagagatc ttccaagaat atccagaaat agcgattgtt gggagtaata
     2041 ttgaagaatt tactggcaca cttagcaatg ttatcggaag aaggattgta cctgagtata
     2101 atgatgaaat ccgtgtgttt tcgaaaagaa gaaacccttt taatcatatg acagtcatgt
     2161 ttgataaaaa tgctatttta gaagtgggaa actatcgccc attaattgga tttgaagatt
     2221 attatttgtg ggtaagacta ttgaaggcgg gatataaagg atataatatc caagagattc
     2281 tagtacatgc tagagcaggt gctgacatgt atgctagacg tggaggaagt aaatatcttt
     2341 taccaggaat taaaggccga tatcgcgtat ggaaagatgg tctaggatca ctaaaagatt
     2401 ttctgatagt tatttgcgga catcttgtaa ttagtttatt accgaataaa attaggggaa
     2461 aagtatatga atcaaaactt agaaaaacaa actaaacttt ctataatcat acctttttac
     2521 aatgcaaaga atactttaag aagagctgta gacagcgtct taacgcaagg ttatcccaat
     2581 attgaaatct tgttaattga tgatggatct acagatggta gtagtgacat tggtgacgaa
     2641 attgctaatc tagagagtaa agtagcatgt tatcacttgg aaaacgctgg tccatcagtt
     2701 gctaggaact atgggataaa agaagcaact ggaaatttgg taggattttt agatagtgac
     2761 gattattttt tacctgattg ttttaataat gttttgaaag actaccatag gaatcacccc
     2821 gatatcattg cttttggatt aagtaaagga aaaaatatag aaaatgcaaa tttatttgtt
     2881 cccgaaaaga agattggttg ttcaagtgaa gaagcaattg agcaaatgtt tagaagtaag
     2941 gcagtggact tttacgtatg gaataaattt tttaaaaggg aattattcaa tcatatagtt
     3001 tttccagaaa ataaattata tgaggatatg gttcctatgt atgaagcttt tagggcggct
     3061 agtaaaattg atattttgcc attttcaggt attttttatt atcaaaataa cgatagcatc
     3121 gtttatcagt cttttaattc aaaacagtat gataatattt tgcagagaaa aatattagtt
     3181 gatgaagtaa agaaagatta tcctcgactt attaatttgg ccaatgctcg gttagtagac
     3241 ggatatcttt caacagggtt taaaattact ggcaaaaatc aaaatcagaa agaaaaacaa
     3301 aaatattata atcagtctaa aagagaaata aaaagtatat attcaaaaat aagaagtaac
     3361 aatgag
//
LOCUS       DAKBVG010000222         3338 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000222 DAKBVG010000000
VERSION     DAKBVG010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.224"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            161..373
                     /locus_tag="OUD09_002444"
     CDS             161..373
                     /locus_tag="OUD09_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693673.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(651..2450)
                     /locus_tag="OUD09_002445"
     CDS             complement(651..2450)
                     /locus_tag="OUD09_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCT9693674.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2574..3170)
                     /locus_tag="OUD09_002446"
     CDS             complement(2574..3170)
                     /locus_tag="OUD09_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCT9693675.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1218 a          602 c          587 g          931 t
ORIGIN      
        1 acaacgtcta agatcgtgtt aaaaaatagt tatctaaaaa gcagaaagat actcaaaata
       61 aaaaataaaa aaatggataa aaacgttttc aaatacaagg taatttgtta ttataaaata
      121 aagaaaaaac aaaataaaaa taaagaaaag agggaatgaa atggtaaaag cagaagtagg
      181 aagatcttat atatgcaaac cgatcggagt atctcatgaa ctaatcggct gtattgaacg
      241 tacatatatc aacacggctg tcgtgtatgt tgaaagttat gaccaacgag actatcaact
      301 tatcaatgaa agcaagtatc gcatgctcgt aaaattctgt gatattagcg cacctgctga
      361 attggtttct taaacagtat ttttgagaat tcgcaagaac tcccttataa tataaataaa
      421 gacagaaggg agcgagaaga tatgccaaaa agaaaaagtt cagggaaaat gggaattatt
      481 cttgcagtga tctctgcaat tcctgctatt ttagctattg ttcgtgcgat gaaggaatca
      541 agagaaagtt aagcacacat tatgagcgtc aaacaaaaaa cgcatatacc tgcggtggca
      601 ggttatgcgt tttttgttgt ataaagactt ttttcattaa cgatttttga ttagccgact
      661 cccaaaacaa aatttagcag tttatctgaa taagtgattt cttcatctgt ttgtttcaca
      721 ctttcattta gtagtcccat ctcatcggtt atgataccat ccacaccata aaacatcatg
      781 cgactcatag catctgaatc gttgattgtc catacatata cttcttttcc gtcttggtgg
      841 gcagaattaa tgaagttacg gttgatcgta ctatattcca ttgtcagaaa atcagcaggc
      901 gtcacagggg gcccaacaat attgaaaggt aagatgtagc ccacataaaa gtttggttct
      961 gcttctttta aatctgaaac ggtttggtac gtcaaacttt gcaaaatatg atggttctct
     1021 aagataactt tgcggtattt atggacaaat cgttcgacaa ggtcatccga atctttttta
     1081 gaggtcttga tctcaataag taatttttgg tttagcttat gtgctttttc caaataagaa
     1141 tcaaaagaac aaacaggggc ttgcactccg ttttcagtaa ctgtaagatt tgtcaattct
     1201 ttcaatgtaa gatcctgtgg tgttccattt acccctgtta agtgacgaag atttgcatca
     1261 tgcatcacta cgaaatgacc atcttttgtt tcctgaacat ccatctctac ataatcagga
     1321 tgatagtctc tgcttgtttt ttcaagtgct tctaaagtat tttgcgcccc attttgggta
     1381 ctcacacctc tgtgagagac agtcactggt tttgtctgaa cagctgaagt cagataatcc
     1441 atattgtaaa gacagacgcc aataccgaaa aaaactgcaa aaagagtcaa accagtgttt
     1501 ttcagcgcag agaaacgcaa gttgggggct tgtgggataa accacttagg gatctcaggc
     1561 aagaagcctt cgtcatccat gaaatcaaca atgatataaa aaatccctac ggtagacatc
     1621 acgatattga ataataaaat cccttgtaaa agcgtcatag caaacacagc gctgatcaat
     1681 gcatagtctg ggaaaaattg ttcaaccata ctttgtgcca aaataacaac gatatatcct
     1741 aaagaagaaa ggagtaaaat cgtaccgcca atcacaataa attggcccgt gatggccaaa
     1801 agacgcgatt tggtcaatga ccagctttcc cgaatggctg ctcgaaaagg acgatcccgt
     1861 agaatcattt ccggtaaagc aaaaatcagt cgaatcccaa tatagccaag gaaaatataa
     1921 acgagtaaaa aggaactgac gatgatccaa cgattagcaa aaataaaatc cataatgaaa
     1981 gcggggattt ttatttttga cagtagatca gaattaaaac ttaatccgct gatcggtaga
     2041 atcaataaaa aataagcaag gaaaaacaaa aaagtaatgg gtctcacttt cttcagctga
     2101 agtatcgtca agtgtaagag ttgcttcaaa gagattggtt ctttcttttt aataaaaaaa
     2161 acactcatca aaagaaaagt aaattcgaaa taaactaaca gtaaaatcgt catcaaaact
     2221 acgattaacg agagtagaac cccaggatgt tgagaaaaaa ttgaaggaat attgtcataa
     2281 gacaaatagt caattgcccc tcggtgtaaa ataaaacggg tcgtactgct caataagggt
     2341 aaacaaataa ataaaataaa gccatgcatc agcaagacat cacggaaata agcttgtgtt
     2401 ccttttaaaa agtcccaagc attacctaag ctacgtttca aataattcat cctatcacct
     2461 gctcttcaca ttatagcgca aatctaatga aaaaggaaag ggtgcagtgt gttttgcaga
     2521 ccctttccca gatttaacat tgtcaatcaa tgactaattt tacaaaataa atattatcca
     2581 agaagaccac ttagatattg acctccataa atcaaggcca tactatataa gaaaatcgac
     2641 agtggtttag caatcaaaat gattagtgta tatcgtttca ctgacatgtt tgtcaatcca
     2701 gccatcaaac ataacgcatc gtcaggagca actggaagaa aaatagctag agcaaacagc
     2761 ttctcaaaac ggttttgatt atccagccag ccaatatatt tgttatatgt cttatcgcta
     2821 atcatggaaa gtatgaatgg ctttccataa cgacgcccta gcagaaaaat gataatcgaa
     2881 ccaatgcaga ttccaatata gttatagata aatccagcat aagggccaaa tatcaggaca
     2941 ccagctgcag tactgatccc tccaggaatg attggaataa ctacttgcag gatttggatg
     3001 aagacaaaaa taatcggtcc taaaatgatg gaatcaccta ctaatcctct taacgcattc
     3061 aaatctttga aaacacctaa attaataaaa taaatcgtca gcgcgattga taggcctaat
     3121 ccgataatgg atatgaaatt tatcagtttt cgagaggcag caatattcat tattcatcaa
     3181 ctccttagag gttctttgtt aactatactt tactagaaaa gcaactaact gcgccatttt
     3241 ttgatatgac agaaaacaaa caattttgta agttcatcat aagtataaaa gattgatctc
     3301 aaaaaaacat ccaactaaag acttatttaa taacttgt
//
LOCUS       DAKBVG010000223         3256 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000223 DAKBVG010000000
VERSION     DAKBVG010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3256
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.225"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(58..633)
                     /locus_tag="OUD09_002447"
     CDS             complement(58..633)
                     /locus_tag="OUD09_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCT9693676.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(638..1612)
                     /locus_tag="OUD09_002448"
     CDS             complement(638..1612)
                     /locus_tag="OUD09_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleosidase"
                     /protein_id="HCT9693677.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIASALKVNPNIGKHMERFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEEAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(1636..1680)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1895..2863
                     /locus_tag="OUD09_002449"
     CDS             1895..2863
                     /locus_tag="OUD09_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCT9693678.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     gene            2960..3253
                     /locus_tag="OUD09_002450"
     CDS             2960..3253
                     /locus_tag="OUD09_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCT9693679.1"
                     /translation="MRSAAYGNQRRKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTF
                     VDPAYRGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
BASE COUNT         1071 a          612 c          645 g          928 t
ORIGIN      
        1 aataaatgaa aaaacagagc cagattagca ttattttgct aatccagctc tgtttattca
       61 gcctattttt ctttttgaat atatcaagcg ttgtggcaac atagtgatga ctaatgaaac
      121 aacgacgata gacaacaaac gatcaaggta gtctgtacct gcctgtacca agatcacact
      181 tgttgtctga cttaaaccta acccatgtaa tagctgaacg atcatactgg atcctgagga
      241 ggtgatcccg ccaaataact gaacggtaat gatcgaagaa acgatcgttc caggtaccga
      301 caaaatcaaa gcgagtaata acagtttcaa gctgttttta tctttgccga gatatcttgg
      361 caaaatcaat ccagcaagca agcctgtgag aatttggatt ggcgaataat acagagaaaa
      421 aatatctgta gtcactccac ttaaaatacc gctggacaat cctgtcagca ttccaggtac
      481 aggcccaagc aatgcaccag caaaaatcgt tccgatagaa tcgagataga ttggcaaacg
      541 aagccataaa gcaatcgttc ctccaatata attgatccca atacacaaag ccatcactgt
      601 aagtacgcga gttgccattt tgtttttatt cattttgtta gcccatcttt aacctttcca
      661 aatctttcat aatcgtttct tcttgtgcat ttaaaagaac ggttaaaaac tttcggaaaa
      721 aaagacgagt atctactttt gtcataattt tactgttagc aggtttctgc caaaattcat
      781 aacgatcaac caatgtttgt ccacgactta ttccttgagt ttccacagca gtgaagcttt
      841 tgaaaccttc acagatggcc tcctcgataa aataagcaac agctaacgga tcattgatga
      901 cacatcctaa aattctttct tgtttccagt ggaagtcaaa ataaaaacga gtgatcgctt
      961 ttagatactc tccctcttca ggattcattt gacagcagta ttcgaggata gtcggtgtca
     1021 agacgatttc tcttgtcaca tctaacccaa ccatttctat cgtttgtttg agattctcga
     1081 atacataaga agctgcttca ggatcacacc agtaattgta ttcagcaact ggcgaacagt
     1141 taccatggct tttgtaagtt ccacccatcg agacaaaacg ttccatatgt ttcccaatat
     1201 ttgggttgac tttcaaagca gaagcgatat tggtcaaagg acccaaagca atgactgatg
     1261 tatttgtctt ttctttgaaa taatcagcca aaaaatcaac ggcatggaga ggttctgcct
     1321 gttttctcaa gatcatcggg aaatttgttt cccctaaacc atccatacca tgagtgtctt
     1381 gtgcactgat gaaaggttgc ttcaaaggtt tttccattcc ttgataaaca ggaatgtcta
     1441 aacgatcaca gcgttccagg catttcagca cattctgcgt acctatatct gcagggacat
     1501 tcccacaaac tacggtaata gcaactactt cgatatcagg ggatttgaca gctaatgata
     1561 aagctaaagt gtcgtcgatc cctggatcgc aatcaataat tacttttcgc attatttcca
     1621 atctcctttc tttagagata cttagttttt tattctggga agtttccgaa ccagtccaag
     1681 acgagcgtct tgattataat gcacgaaatt agtatactgg cttttattgc gaatgtaaat
     1741 gctctcttac aagtaaatct attgaggaga actttaagta aatgatacaa tgaaaaagaa
     1801 atggaattta cgtgtgccga aaataaagaa tcctatggat cggtacattg gataatagat
     1861 agattatagt ttggcagaaa gcggcgggtg tacaatggtt attttttatt tcattcttat
     1921 tctgataatt atagggatca gttaccattt tctttcttat ttttatgcaa agaaattagt
     1981 aagagcggga gtacagagag gatatgactg gtatgaagac actggtcatc gtttgatgga
     2041 ccctgctgca gtcacgtcag tagaaaaaaa acgacaaaaa ttagaagaag agtgtgaaag
     2101 cttttgggag cagggaacac ccaaaacaat caaaagttat gacgggttga aactagctgg
     2161 tcagatgttc atccatccaa ataagcagaa taaatgggtc atttgcgtac atgattaccg
     2221 aagcacaggg aaaagagaca tgtctcatat aggtaaaaga tatgcagaga aaggattcaa
     2281 cgttcttgta cctgatttac gtgcgcacgg agaaagtgaa ggagaaatca tcggaatggg
     2341 atggctagat cggctagatt taattgcctg gatccaactc atcttagatg aacagccgaa
     2401 tgcgtccatt attttacacg gcggatcaat gggagcttct acgatcatga tggcaagtgg
     2461 tgaaaaactt ccatcagcgg tcaaaggatt tattttagac agcggatatg tttctgttta
     2521 tgcagaattc cgatatatgt taagtaaaat cactgtcttt cctaaaaaaa tggtcatgag
     2581 atatgcgaat cattatgccc agaaatacgc cggttactcc ttaaagcaag catcagctac
     2641 gagacagctt ggcagcaatc atctgccttt attagtcatc catggtgaaa gagatcattt
     2701 tgtccctaca gaagcagcgt acacgattca aaatgctacg gcaggggata aagcactgct
     2761 gcttgttccg gaagcagaac acttggaagc atcgatgaaa gatccgaaaa cctattggac
     2821 agtgattttt tcatttattg aacaaagaat aacgatgaat taattttctc ttcttttttg
     2881 taagtaattc gaggataaat aattgtttat agatcaggag actgctaaaa taaggtagga
     2941 ataaaaaaat atcagttatt tgaggagtgc ggcatatgga aatcaaagaa gaaaaaatcg
     3001 tcttgcttta ttcaatgatg aacaacaaga gatcggagaa atgacatggt cagatgcggg
     3061 cccagacatc atgatcattg accatacatt tgtagatcca gcatatcgtg gtcaaaagtt
     3121 agctgaaaag ctagtgtata ctggagtaga attagcaaga cgagacggga aaaagatcat
     3181 tcctttatgc ccttatgcaa aaaaagagtt tgaaaagaaa ccagaatatc aagacgtctt
     3241 acgtcagtca taagaa
//
LOCUS       DAKBVG010000224         3226 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000224 DAKBVG010000000
VERSION     DAKBVG010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3226
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.226"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            <1..1162
                     /locus_tag="OUD09_002451"
     CDS             <1..1162
                     /locus_tag="OUD09_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCT9693680.1"
                     /translation="VDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNG
                     SQLRTSLLTIAKEWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAK
                     EGFSNMSIFQTAKGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKA
                     DTMKVTTCLNPLHTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGI
                     IDPKIFLEEVITVRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLV
                     FIPLTLAAWLRYLIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPI
                     LSNKEIFGVDLYQAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            1153..2235
                     /gene="uxuA"
                     /locus_tag="OUD09_002452"
     CDS             1153..2235
                     /gene="uxuA"
                     /locus_tag="OUD09_002452"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCT9693681.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(2343..>3226)
                     /locus_tag="OUD09_002453"
     CDS             complement(2343..>3226)
                     /locus_tag="OUD09_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HCT9693682.1"
                     /translation="CKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQ
                     RILVNILARLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKE
                     KILYSLAFARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQ
                     HLLPVILEKENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSN
                     ESIKIQNDFMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFEL
                     KEEALMDQSKKENQVTS"
BASE COUNT         1044 a          559 c          642 g          981 t
ORIGIN      
        1 agtcgatata aaaaatggtc caaagaagcc atcacataca atgagtattc ttgctgcgct
       61 actttataaa cgttttttat ctggagcttg gccgattgca cttgtctcta cagataattt
      121 ttcacaaaac ggtagtcaat tacgtacttc tttgctaacg atcgcaaaag aatggcaaaa
      181 ggaaggttat gtacaaaata catttatcca atacttatcg aatgaagaaa ttgttgcgtt
      241 tccatggacg atgattgatc gtatcacccc gaatccttcc ttgtcagtta gcaaaatgtt
      301 ggcaaaagag ggcttttcaa atatgtcgat tttccaaacc gcaaaaggaa ataatattgc
      361 tccatttgtc aatacagagg acatccatta tttaatcatg gaagatcatt ttcccaatgg
      421 acgtccgaaa ttcgaaaaag ctggagtatt cttgactgat cgtgcaacgg tagataaagc
      481 agatacgatg aaggtgacga cttgccttaa tcctttgcat acgtctttag ctattatggg
      541 ttgtttactg gggtatagga gcatagctga ggagatgaaa gatccagatt tatcgctgct
      601 agtttctgaa atcgggtaca atgaaggcct cccagttgtg gagtctccag gtatcattga
      661 tccaaagatc tttctggaag aagtaattac tgtgcgcttt tctaatccaa atattcctga
      721 tatgcctcag cgaatcgcca cggatacctc tcaaaaggtg gcgattcgtt atggtgagac
      781 gattaaaaaa tatatggaaa tagaagttaa agacccgcag acacttgtat tcataccatt
      841 gactctcgct gcttggcttc gataccttat tggcattgat gatcaaggaa aaccattcat
      901 cccgagtcca gacccactat tgacagactt tcagcataag ctttccgata ttcaactggg
      961 tacgcaagat acatcacata ttcatgagaa aatggctcct attttgtcaa ataaagaaat
     1021 ttttggtgta gatctttacc aagcagagct tggcagaaaa atagaagaaa tgttcatcaa
     1081 gatggtagca ggaccgaatg ccgtacgaaa aacattacag gattacttac aagaacatgg
     1141 aggaaattta tcatggaaat gacttttaga tggtacggat cacaggaaga aaagattacc
     1201 ttagatgaaa tcagacagat tcctgcggtc aaagggattg taggcacatt gatggatata
     1261 ccagtaggag aggtatggcc tagggagagg atcaaagaac taaaagaaga aattgagtct
     1321 cgtgggttaa cactaaaagt aattgaaagc gtcaatgtgc atgatgccat taaaattgga
     1381 ttaccttccc gtgagcagta tatcgaaaac tataagcaga caatccgaaa cttggcagaa
     1441 tatggaatag aagtcatctg ctataatttt atgcctgtat ttgactgggt gaaatctgac
     1501 cttgactacc gcttagaaga cggctcatcc acgttagctt ttatatctga tgatattcca
     1561 gatgatccaa gagaaatcgt cgcgcgcatc gaacattcca gcaatgaatt tgaattgccg
     1621 ggatgggaac ctgagcgctt agctcatatc aaatccttgc ttgaagccta tgcatcagta
     1681 gacgaagaaa aacttcgaga aaattttgcc tattttctcc aatcaatcat cccaacatgt
     1741 gaagaggtag gggtcaaaat ggctgtacat ccagatgatc cgccatactc gatttttgga
     1801 ttacctcgaa tcgtaaagaa tcgagaagat ttagattggt tgtgccgcat agtagacagc
     1861 ccttataacg gaatcactct ttgtacaggg agtattgcag aagatccaga aaatgatgta
     1921 tatgggattt tagcagaatt ttgtcaaaga gatcgaatcc catttgcaca tgttcgaaat
     1981 attaaatttt tagctaatcg agatttctat gaggcaccgc ataaatccga atacggttca
     2041 ttagacatgt accgcatact aaaaacaatg tatgacaacg gatttaacgg atacatccgt
     2101 cctgatcatg gaagaatgat ctggaatgag acaggaagac caggatacgg cttatatgat
     2161 cgagcattag gtgctgttta tctaaatgga ttatgggaag caatcgaaaa aggaaacaaa
     2221 agtaaagata attagtgaaa aaaactgtaa ttttattatg taaataagaa ggatgttcaa
     2281 attcttgata cataaatccg gaggttgtta ataagcattc ggatttatgt atcttatttg
     2341 gtctaggaag taacttgatt ttcttttttc gattgatcca tgagtgcttc ttcttttaat
     2401 tcaaagatat attctgagat cctcataatc acttttttcc agtcttctgg tgtgatgatt
     2461 ccttttaaaa tatagatttt tttgatatga gaaggcaatt gtttcaaagg gcagtcgctg
     2521 ataacgatgt tgcagttctc cataaaatca ttttgtattt tgatcgattc atttgaaaaa
     2581 atttgttcta tcttaacgat cagatcttta ctgatataaa aatcacgcgt atagttcaca
     2641 ccaatcacaa ttcgcggctg ttttttgtag cgatcataca aatgtactag gtcttcaacc
     2701 atccattgca cattttcctt ttctaaaata actggtaaca agtgttgatt ttgttctctg
     2761 aaatccatat aaaattgttc aatggcggtt tggacttctt tcaacacgct atgttcttga
     2821 taaagcatga aagtaggaag gggcgtattt ggttgattga agttaagaaa tccattataa
     2881 agtttatttc gtgcgaatgc caatgagtaa agaatctttt ctttttcagg tataaacaaa
     2941 tcaaatacag tacaaaagga ctccaccatt tctcttgaat aaagaacatg aggaacgttt
     3001 gcttttttta aaagtttgta ttgctccaag cttgtttctt ccgattcgat tttcgtgata
     3061 gaaagtcgag ctaaaatatt cactaagatg cgttgttcgc gacaaattgg cagattaggt
     3121 cgcaaaatat ctacagaggg atcaataaaa aagtcaatat attcatactc tgtgaattct
     3181 ttttgtacgt ctttcacaga agttgcagga aaattctctt tacata
//
LOCUS       DAKBVG010000225         3188 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000225 DAKBVG010000000
VERSION     DAKBVG010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.227"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(117..614)
                     /locus_tag="OUD09_002454"
     CDS             complement(117..614)
                     /locus_tag="OUD09_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HCT9693683.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(744..1454)
                     /gene="budA"
                     /locus_tag="OUD09_002455"
     CDS             complement(744..1454)
                     /gene="budA"
                     /locus_tag="OUD09_002455"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HCT9693684.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(1467..3131)
                     /gene="alsS"
                     /locus_tag="OUD09_002456"
     CDS             complement(1467..3131)
                     /gene="alsS"
                     /locus_tag="OUD09_002456"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HCT9693685.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
BASE COUNT          885 a          712 c          520 g         1071 t
ORIGIN      
        1 tcaataacac ttgtgatttt ataaaaaaat aaaaaaaccg aaaaacagaa atgcccactg
       61 tctttccggc tctttttatt cttgatttct cttctttttt gtttcaatag acgattttag
      121 ttattaataa atgaaaaagc atttatatta aaatttgcta ataaaagatc tacatatttt
      181 tctagaaatg cttgatcgat ttcatcatac cctttcgtta ctccgcaatc gatatctaaa
      241 acaccgatta aattaccttc ttttaccatt ggcactacga tttctgattg tgcggctgaa
      301 tcgcaggaaa tgtaatttgc atgggttttg acatcatcaa cgatcaatgt ttcttgtttt
      361 tcagctgatt ctccacaaac tccttttccc atcttgatac gtgtgcaaga aactcgccct
      421 tggaacggtc ctaaaatcaa ttcattacca tcgaataaat aatatcctgc aaagacagtc
      481 tcaggcaaag tctcattcaa caatgcagat gaatttgcta aatttgctaa ccagttatct
      541 tctgcttcta ataatccttg ttgctgtaat aataaaattt cgtatgcatc ttttttctgt
      601 ttttctgtcc acatgataaa aaccgctcct ttttgatcta tggagaagat tatagctcag
      661 tttttttgta aacgctaggt ttcctcctca aaaaaacgag atacgtcagg ttaatcacct
      721 tgaacgcatc tcgctgtctt tttctattct gcttgttgga tttctgctgc taaatcagca
      781 taatcaatcg tttcgtgaac aaaagtcgga tcatcaactg gaaaatgctg tacaagggac
      841 gagatgcggc taatctcaac ttttccttca gccacttcaa aatccattac atgtccgcca
      901 aatgtatgtg tatcatcgat aaaatgcaag tgaaatcccg ctgcagcaac tccttggaat
      961 aattcaggcg taaagaaccc gactaatgtt ccttggacat tgctttgttg gaattctggt
     1021 tgagactctg aaatttttgc tagtcttctg taaggttttt cttgcttcgg catgatacgg
     1081 atgtgcatat ttttgaataa tccagttatc ttcactgcca aaaataggtt ttccccggct
     1141 tctcttacta atatgtcttt tttcaattgt tctccatctg cagtttttga gacagaaaaa
     1201 ctagtatctg gcgtaaaatc tgtaattgct gcataggggg taagttcact tccatctaat
     1261 tgggcaactg agccatctga tcttccttga taagctacgc catctaaaaa gatgacttca
     1321 ccatcaagcc catgcaatgt tcccaatccc agagttcctt tttctaatat atgagcaatc
     1381 gtttctgttc catccatcaa gccagccatc aaagctccta atgtcccgtg ctggtataag
     1441 attttttcag tcatgtcgac acttccttaa tagaattgat ctggtaagat tgtttctccc
     1501 aatttctcat tatcacggta atcaatcgga atatcaataa ttacgggtcc ttctgtcgca
     1561 aatccttctt ctaaagcttt ttctaattcc tctacacttg tagctcggat tcctttagcg
     1621 ccaaaagctt ctgcatattt cacaaaatca actggaccaa agtctacacc tgacgcccgc
     1681 tgatatttca ttttttcttg gaactctacc atattgtagt gtccatcatt ccaaatcaaa
     1741 tgaacgatat tcaatttttt acgtaccgcc gtttctaaat cctgggcaga gaataaaaat
     1801 cccccatcgc cggaaactga tacgatttgt gtatttggac gcaccaatgc agcagaaatc
     1861 gcccatggca atgcaacacc aagcgtttgc ataccattac tgaataacaa atgacgaggt
     1921 tcataacttc taaagtggcg tgccatccaa atataatgac tacctacatc tacggtaaca
     1981 gtcatatcat ctgttacttt tgactgaagc gtgttaataa cttctaaagg atgcaagata
     2041 cctgcttctc cttttgattg aacaatgtcc cgctccgtta attctgcttg aagagatgct
     2101 aaatattctt ttgcatcttc tgacacttgt tgaggctcta atgaacctgt caataaatca
     2161 agagtggctg agatatcacc gatcaattct ctttcaggct gcataaaagg atcgatttca
     2221 gctggcgctt catcgatcac aatgatccgc gcatcttttt ccgcattcca gtttcttgct
     2281 tcatattcga ttggatcata gccaatagca ataactagat cacttcgttt caaaagcata
     2341 tctcctggtt ggtttctaaa taaacccaca cgaccaaaga aatgcgcttc taattcacgt
     2401 gagatcactc cagctgcttg gaatgtctct acaacaggca atgctgtctt tccaactaat
     2461 tgcctgatgg ctaacgtttc tttttcactt gatcctcgca tacctactaa tagtaccggt
     2521 agttttgctt ctctaatgcg ttcagctagg taacggatat catctgcaga agcagaacct
     2581 aatttaggat cgctcaatgg cttgatgaca ttcccttgga caggtgcatc cactacatct
     2641 tgcgggatac tgataaagct cgcgcctttt tttgaactct tagccatacg ataagcattg
     2701 gcaatcactt ctgaaattgt ttctggatct tggatttctg cactgtattt tgtgattggc
     2761 tggaacagtg ctgcattatc catgctttga tgggtcaact tcaatagatc gcttcgtttt
     2821 acttgtccgg caattgctaa aactgggtca ccttctgctg tcgctgttac caaacctgta
     2881 gctaagttac tagcaccagg accacttgtt gcgataacta ctcctggctc tcctgtaata
     2941 cgtccgattg cttgtgccat aaatgcagca ttttgttcgt gacgagtcac gattaattca
     3001 ggaccttgat cctctaattc attaaataca ccatcgattt ttgcacctgg tataccgaat
     3061 acataatcaa catgatgatt aatcaaactt tctactacag atttcgaacc ttgtttcatt
     3121 ttttcgtcca ttgaacatcg tcctttttat gctttatttt taattattag ttagaaataa
     3181 tcacaaat
//
LOCUS       DAKBVG010000226         3181 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000226 DAKBVG010000000
VERSION     DAKBVG010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3181
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.228"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(304..1320)
                     /gene="tsaD"
                     /locus_tag="OUD09_002457"
     CDS             complement(304..1320)
                     /gene="tsaD"
                     /locus_tag="OUD09_002457"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCT9693686.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(1317..1781)
                     /gene="rimI"
                     /locus_tag="OUD09_002458"
     CDS             complement(1317..1781)
                     /gene="rimI"
                     /locus_tag="OUD09_002458"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCT9693687.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(1788..2330)
                     /gene="rimI"
                     /locus_tag="OUD09_002459"
     CDS             complement(1788..2330)
                     /gene="rimI"
                     /locus_tag="OUD09_002459"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCT9693688.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(2314..3036)
                     /gene="tsaB"
                     /locus_tag="OUD09_002460"
     CDS             complement(2314..3036)
                     /gene="tsaB"
                     /locus_tag="OUD09_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCT9693689.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
BASE COUNT          889 a          709 c          547 g         1036 t
ORIGIN      
        1 atgattaaaa gtatgtaaaa aatagggctt tgacaaaaac gtgtcacagc cctatttcga
       61 ttaaacggtg tgtacctgtc aactcttcgg tcacaagtca taatgttcat aaacatgagg
      121 agctgttttt tgaaattgtt ccttgtgaat cagagttaaa cgacaggctt acgaactctt
      181 gattaaacgg cgttcaaaag cttgcatacg cggtaataag aacagccagg tcaaaaacat
      241 gagaagccgt ttttcctggg ctgttcttat tacttgatgc agggcagctt tttcacaacc
      301 tctttattca gataaaacca agcttggatc agcattcaac tgatagctag caaaatttct
      361 tttttgcatt tccacatgtg ctgctgctcc aatcattgca gcattatctc cacaaagtcg
      421 aagaggggga atcactagtt ctacctcagg gagtttagtt gccaaagcat cctgcaatcc
      481 ttcacgcaac ccttgattgg ccgctacacc tccagctata atcagttgct tgactggata
      541 attttgacaa gctcgaagtg ttttagtaat caatacatcg atcacacttg cttgaaagct
      601 agctgctaaa tcatttttgt ctagatcttc tccacgctgt tgtgcattat gcaccaaatt
      661 gataaatgca ctttttaatc cgctaaaact gaaatcataa ttatcttcat ggatcatcgc
      721 tcgcggaaaa tggtaattat cttttccctg atgagctagc tgatcgattt cttttccgct
      781 tgggtaagaa agacctaata ctcttccaac tttgtcataa gcttctcctg ctgcgtcatc
      841 tcttgtctcc ccaatgattt catatgatcc atcttcttgc atatagacta attctgtatg
      901 tcctccgcta acaagaagtg ccatcaatgg aaactggaat ggtttaacca gacgggctgc
      961 atagatatgc cctgccatat ggtttacagg aataagcggc aattggtgtg cccaagcaaa
     1021 agccttagct gcagaaatac caatcagcaa cgagccgaca agtcctggtc cataagtgac
     1081 agcgactgcc gataaatctt ctgccgacac accagcttct accagtgcat cttccaagca
     1141 taaggtgatt tgttctacgt gatggcggct agctacttca ggtactacgc caccaaaacg
     1201 tttatgactg tttatttgag aagcaacgat gttagataag atctctgttc catttctaac
     1261 gaccgctaca ctcgtttcat cacaacttga ttcaatcgcc agaatcaatt cttcattcat
     1321 ttcatcgtct ccgtttttaa ctttctgctc ataatgatcg catcttccgt cggattgcga
     1381 taatatgatt tccgtttacc taatatacga aacttttctg attcataaag ttttcttgct
     1441 gcttcattag atagacgtac ttctaaaaaa acatgcagcg aatcttttat ttgttccgta
     1501 atcagtaatt gtaacaaatg ccgtgcatgc ccttttcctt gttctgaaac tgcaacagca
     1561 atatttgtga tctcgacttc atctattact ttcgagtatc caataaagcc acagatctgg
     1621 ttatcttcag ctaaaagcag ataatcagag tgaggctgtt ggatgtctgc caaaaactgc
     1681 attttcgtcc aaggtgaacc atgtggataa gcattttcgc taatcatcca tacgcattct
     1741 gccaagtacg cttcatcaaa atcttttttt ctgcaaatca actaatctca ctcattttct
     1801 aaaagtttta tcatatccaa cgcatcttct tttgtttcag tgtaatatct ttttttgatc
     1861 gttgttgctt gaaaccccaa tttccgataa agtctttgtg catcttgatt actcatacgc
     1921 acttctaatg acatcgtctc acagcgattc attatagcaa agttctcgat ttcgtcaata
     1981 agcagcgaac cgattcgttt acgctgatat gccggtcgaa cggcaatatt cgtgatatgg
     2041 caatctaaac cgaacgctct tgaaccgata aaaccaatca ttcttccatt atcaaagata
     2101 cctagatata gatgaggaag cggtgacttc aattctgata taaaggcact ttttgtccat
     2161 ggcaattcgc cattatagac ttctcgttcg acatccagca gctctttgat atcttctgta
     2221 gtcaattctc ttagtacgta acgacgatcg atttgttcga ctgttttttc tgtataaatc
     2281 attttatctt gaaaaaagct tctggcatac gctctaaatt ttttcaacat aactttcgtc
     2341 tccaggtgtg tgcgtctcca accatttttc ttctgcttct actcgtttta aatagcgagg
     2401 aagaaaagct tgtttctctg caactggttc aactaaagca cctaattgag ccacagttac
     2461 accacttgga atatcccatg ctggaactgt attaatcatc gcactaggca atatttcctt
     2521 tatttcagag aagaatttgt gacaatcact cccgacgaaa taaatagctt cattcagctt
     2581 ttgtagttgt gtgaacaaat cagaagcaga aatatgtaca tcttcaagta cagtttctaa
     2641 tctattttct ttccagcggt atgccccagc gtatacattt tttcttcgag catcaaacat
     2701 gggcacaata atattatcca caccgatgca attagccgcg atggttttca gactagatac
     2761 tcctactagc tctttatcta atgtataagc aatcgttttt gctgtcgtta cacctatacg
     2821 aagccctgta taagaaccgg gaccatcaga aaccgcgatt cggtcaatgt cttttggact
     2881 tatttgaaga tctgcaaaca attggtcgat tgcaggcatc agtgttacac tgtggttgcg
     2941 tttgatattt gtttgtattt gtccaagaat ttgttcgtct tctaccacac cgatcgccaa
     3001 agtttggttt gcagtatcta ttcctaatgt aatcatatat ttgatccttt ctgctatgac
     3061 attctcgcct tccattgtag catacttttt tattctgagg atgaaaatac caaaggcaaa
     3121 actagtacaa taatacatga ttatctgctg gcgggcaaat tttgcccctt cctgaaacac
     3181 a
//
LOCUS       DAKBVG010000227         3136 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000227 DAKBVG010000000
VERSION     DAKBVG010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.229"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            92..1609
                     /locus_tag="OUD09_002461"
     CDS             92..1609
                     /locus_tag="OUD09_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HCT9693690.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            1581..2870
                     /locus_tag="OUD09_002462"
     CDS             1581..2870
                     /locus_tag="OUD09_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCT9693691.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            3045..>3136
                     /locus_tag="OUD09_002463"
     CDS             3045..>3136
                     /locus_tag="OUD09_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCT9693692.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTV"
BASE COUNT         1070 a          464 c          688 g          914 t
ORIGIN      
        1 tcgataaaaa ataagtaagt ttaacattta tattattgac aacgctttca acgaggtgta
       61 ttattcatgt ataaattaaa ggaggtcagt tatggaagcc acaatgttga ttagtagtgt
      121 caagcgtgta ggcaaagttg atccgcggtt gtacagttca tttattgaac atatgggacg
      181 agcagtatat gaagggattt atcagccaga tcatccatca gctaataaaa gtggatttcg
      241 ccaagacgtc attcacttgg taaaagaatt gaacattccc atgattagat atcctggtgg
      301 aaattttgtg tcaggatttc gttgggaaga cagtattggg cccatagcaa atagacctag
      361 aaggttggat ttagcttggc ggacaattga aacgaatgaa gttgggattc atgaatttta
      421 taactggtgt cagcatgtgg gggccgaaat caatatggct gtcaatctgg gtactcgtgg
      481 agtagatgca gctcgtaatt tagtcgaata ttgtaatttt cctaaaggaa cttattggag
      541 tgatttaagg cgttcgaatg gtcaagagag cccttttgga attaagacat ggtgtttagg
      601 gaatgaaatg gacggtcctt ggcagattgg acataaaaca gcggaagaat atggacggtt
      661 agcagaggaa acggctaagg cgatgaaact cgtagatgat tcaattgagg tggttttatg
      721 cggaagttcg aatcggcaaa tgcctacatt tggtgattgg gagttaacag ttcttgatca
      781 agcgtacgat caaatagatt atttgtctct gcatcaatat tatggcaacc aaaaaaatga
      841 tctttctcat tatcttgcac gttccttaga catggatcgg ttcatttctg aagtgacagc
      901 gatgtgcgat acagtcaagg cgaagaaaca tagtaagaag cagatcaacc tgtcctttga
      961 tgaatggaat atttggtatc attcagcgga acaagatgag caagcagcgc cttggcagat
     1021 tgctcctcct ttactagaag ataactataa ttttgaagat gcattactat taggctgtct
     1081 attaatcact ttattgaaac atgcagatag agtaaagatt gcttgtcttg ctcaattggt
     1141 aaatgttatt gcaccaatca ggacagaaac aaacggagaa gcttggagac agacgatcta
     1201 ttatccattt atgcaagctg ctgtgtatgg acaaggagag gtgttaagcc cacaaattcg
     1261 ttcagcaaac tatgcgacgg aagattttga agaagtgccg tatttagaaa gtatagccgt
     1321 aataaatgac aaagaacttg tcatatttgc agttaaccgt ggagaagaag aaatgaatct
     1381 aactgttcac ttgtctgaat tatcattaga gggagtgatt gatttttctg aaatgagtgg
     1441 atttgataca aaagcaacta atcttcctaa aagtgaacaa gtaaaaccaa gtcattcaac
     1501 aaatgtaaaa gctgaagatg ggaaaatcaa ttgtttattg aaaccgctat cttggaatgt
     1561 ggtcagatgt cgtttatcag atgaaggagg aacaaaaaat gaaaggtaaa aaatggttaa
     1621 ttggctcttt gttggtagct gcaacaggta ttttaggagc ttgcggtggc ggaagttcag
     1681 cagaatctga ttctgataaa gaagtgactt tgactttttg gaatgggttt actgcatctg
     1741 atggtgaaat attgcaagaa atcgttaatg aatttaataa aacaaatgat aaaaacatca
     1801 aaattgaaat ggatatcatg acttgggcca atttgaatga aaaacttcct acagcgattt
     1861 cttctaaaaa agcacctgac tttattgcat taaattatcc agattttgca cagtatgtag
     1921 aaaacggagc ggtacaacca ttagacgatt tctgggaata tgatggagta gataaatctg
     1981 attttaaaga aacggctatt gaattaggtg aagtagaagg taaacagtat tttgtcccca
     2041 tgcaggtaca aggcatgtat atgtattgga ataatgattt gtttaaacaa gctggattag
     2101 atactgaaca accacctact acttgggaag aattgacaga aatggcacca aaattaactg
     2161 atagtagtaa aaatgtatca ggttttgtct tcaataagga tggaactgca ccgttatata
     2221 actggatgtt agcaaatggc ggtaaattag tgaacgatga ttatactaag tctgaatttg
     2281 cgtctcctga gaaccttaaa acgttaaaag cgatccaaaa aatgatccat gtagacaaga
     2341 ctggtccaga atcgatttct ggcgccgaaa tggacaattt aatgaatgct ggtcaactag
     2401 ccattgaaat caatgggcca tggttaaata acggattgaa agcaaacgag attgattata
     2461 atgtgactac cttaccacaa gggagtgatt cagacaaaaa aacagctatt ttagatggcg
     2521 taggatatgc cattccggca agtacagata ataataaaaa agaagctgtc tatgagtttt
     2581 tgaaatattg gaatacaact gaaattggta aaaaatggag tatcgaaaat ggttttcctg
     2641 cctacttgga ttcagtagca aaagatgaag aagtaaaaaa tgatcctatt gtatctgaat
     2701 tatcgaaaca aatggactat gcagagccat atttaccagg ttttacccaa atctctgcga
     2761 tcaataacga tatcatcaat ccactgattg aaaaattatt agcaggagaa gatcctgaga
     2821 cactcatgaa cgatgcagat gatgccatca atcaattact gcaaaaatag tacagaactt
     2881 tatacaggat agactgttgg caagatacag atatctgaat gatcgaccta gtatttgcag
     2941 ataggtgtta ttgcaaaaat cacagcgatt gttcggatat cttattcaga ttcgtttaaa
     3001 ccaagattgt atattttatc aaaatgaaag atgaggtgga ctatatgaac ggattcacca
     3061 aatattggaa tcgtcctgat cgtgctgcgt atttgttttt aactccatct tttttagttt
     3121 tattattatt tactgt
//
LOCUS       DAKBVG010000228         3133 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000228 DAKBVG010000000
VERSION     DAKBVG010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.230"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(20..712)
                     /locus_tag="OUD09_002464"
     CDS             complement(20..712)
                     /locus_tag="OUD09_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCT9693693.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRSGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            707..973
                     /locus_tag="OUD09_002465"
     CDS             707..973
                     /locus_tag="OUD09_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCT9693694.1"
                     /translation="MHRTFGSRGCPTLHLADRYAERKTPTLCFAFSARTRQAGIPLED
                     IKDFLGHKDVSTTQVYAHISPEVKKRSMNQLENYIEEQIKKHSN"
     gene            complement(1149..1718)
                     /locus_tag="OUD09_002466"
     CDS             complement(1149..1718)
                     /locus_tag="OUD09_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCT9693695.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1792..2115)
                     /locus_tag="OUD09_002467"
     CDS             complement(1792..2115)
                     /locus_tag="OUD09_002467"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693696.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVITDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2256..3080)
                     /locus_tag="OUD09_002468"
     CDS             complement(2256..3080)
                     /locus_tag="OUD09_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693697.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFLHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKYFTKNGFVT"
BASE COUNT          866 a          631 c          553 g         1083 t
ORIGIN      
        1 aaagaggggg ggggattttt tatcgttgtg gaatatttaa ataccaacgc ttgtcatgaa
       61 aatcttgtgc tccaccctta gtatttcctt ctggatcgtt tgtcgcacgc atcatgacgt
      121 atactttctt attagggaaa tcacgcatat tgaaagatac atgatagcca acatttccta
      181 aagtattata agcttgattt acgtctggtc tataaatccc atcagctctt actctagcta
      241 gttcttttcc agtattgtag tccataatga aaatatactc gtatttataa ttagcaatgt
      301 gccatccagc tacatgcaag tttgcgtttt cgatttctcc gaactgatca atgtgggcgt
      361 aatttgttcc atctgtcagc gtagaatttg cagcacctga tcgagttgga tcaattactg
      421 gtttatcctc cgaagtagtt ggattatcat ccgtaaaacc atgagccaaa tcataggcta
      481 gtttttcttt acttacaccc atttcagaaa gataaccgta aggatctgta tgatcgcccc
      541 aaatattttg tgttacccat aaatgcgatt tgattcctgg ttggttataa ggagtgtcta
      601 atgttaatgg aataccatat ttcattgctg aatctcttgc caattcaacg tatactttat
      661 agttcttttc aaaagtttct ttatcatgtg tgtgttgtaa ctcgatttgc acaggacttt
      721 tggctcacga ggttgtccta ctctacatct tgctgataga tatgctgaaa ggaaaacacc
      781 aacattatgt tttgcttttt cagctagaac gagacaagca ggaatacctt tagaagatat
      841 caaagatttt ttaggtcata aagatgtttc cactacacaa gtctatgccc atatttctcc
      901 cgaagtgaaa aaaagatcaa tgaatcaact agaaaactat atagaagagc aaataaaaaa
      961 gcactcaaat tgagtgcttt tttaagctca ctatcactaa aactatcact aattttattt
     1021 ataagcgaac atgtttctat cactgatttt ttaaatctag cgaaaaaccc ttatcttaaa
     1081 ggcgttcgtt caattctttg gcaaggtctt caaagcctgg cttgcctaga agtgcgaaca
     1141 tggttttctt atagctcatg tagaaaaatt ttcttgatga tgattttatc catcaattga
     1201 gattgactgt tttgctctcc tatgatttca gctatgattg tccgattttg cggttttctt
     1261 cttaactggt agttgatttt cccagtttcc atcaactcgc taagcatatc cacgactccg
     1321 ccaaatccgg tctttttcgt aataaaacct agtgctacca ttcgttcatc tagatttttt
     1381 gctgtaatcg tcacaccagt aatgagcgag tgctgtaatg tttgggccag ctcttccatc
     1441 ccttcagcat tggatacgac ggaagctgtt ttgttaatag gttcttcgtt tatttctttt
     1501 ggttctttcc tctccacttt cactgtttga cgaaggacat ctagttcttg caagagcgtt
     1561 tcgtattgtt cttgggtcag catcacgcca gctacttttt cacgattaaa tatatatacg
     1621 ccagttcccg cgtctcgcgc ttgagcaaat acatccatcg gcgatcgttt cacttctgtg
     1681 atcgaagatg tgggaacttc taatttcttt aattccaatg gatttcctcc tttactaatc
     1741 gctttttatt atagcacaga ctgacttgtt ttgatctttt ttccaaaggt tttatcggtt
     1801 tttgcctaac cagtaattga tatcggtaag aagtctttcg ataccaaatg aaggatggta
     1861 cagatacgtt ttttgatctt cgttatggtc tagtggaaaa aaatgatcgg tgatcactag
     1921 atctgcatac cgaggcggct tatttcgagt acatacatgt atagagatcc gttttcgatg
     1981 tcttagcgtt ttgttcagta ccccagcaag tcttcttgct tcttggtgtt catagcgaaa
     2041 aaaaaagacg attttcactt caggagggtg gacatctaaa taccctcgtt cctggagtgt
     2101 tttcagtaaa agcatccccc actctactgc ggggtgtgca agtgcttgtt ggatcgctag
     2161 ccgaaattga tatagtttat ctttggaatt gggaaatttt tctaatgttt cctgataata
     2221 acgaacttct tctatcagcc ggtcatggat accaattacg taacgaagcc attcttggta
     2281 aagtactttg tcagctttga tttgctttcg ctctcaaatt cgctattcag ccaaaacagc
     2341 gaatcaagct tttcagtcac aatcgtcaat ttccgatagt cttgtgtctc ttctatccgt
     2401 ttccaccgct tttcagtaat ctggttacag ctcaagcgca aaattgctag ataatttttt
     2461 tcttttattg gcaaaatctg ctcctctcta aatagcaatt caggaaacaa ttcaaagaat
     2521 agtgttcgac tgtctcctaa aatctcgatt tctgccggat cctttctttg cttcaataat
     2581 tggatgaaca tctcagaaaa ggactgctct ccatcatctg caaaaaaatg cagaaaatct
     2641 ttcaaaaagc aaaacagcag atatcttact tttctttctg aaccaaagat ctgtgctttt
     2701 ttataagaaa aagaaaggtg gtactcgcca aaccactttc tcaatcgctc ttgcgtagaa
     2761 cgatcgtact tctgctcgta aggttcattc aatagcagat ttttcagtaa acaaaacggg
     2821 ggtgattgga tcagaaaaac aaacaagaga taaggttcgc tatatccttg atcataaact
     2881 aactgaacgg ttttcttatg aagaatcagc tgaatgggac agtcgctttc cgccaaacgt
     2941 ttcagatgcc gatccactgt tttaacatcg attttcagtt cttcagctaa atcagctttt
     3001 cgccagatca aatctttcat tagattgatt agtgaaaata aattctgtgc attttttgat
     3061 aatagttttt gccatctcat cgatcatttc tcacctgatt tctaaacata ttgtttgatc
     3121 aaaatgggtt cac
//
LOCUS       DAKBVG010000229         3123 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000229 DAKBVG010000000
VERSION     DAKBVG010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3123
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.231"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            <1..375
                     /locus_tag="OUD09_002469"
     CDS             <1..375
                     /locus_tag="OUD09_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HCT9693698.1"
                     /translation="ELIETFIEENLLKQIWGESIVNCLKLASNSDYRQFDNWYKKFKY
                     AIRSAEKEQKIQLKIIYEICNNKYFVDHVREQLSMTLRDLIRRAKTDHRIKQKDDYIF
                     ASLKNKALELIELQISEGIDKQ"
     gene            451..669
                     /locus_tag="OUD09_002470"
     CDS             451..669
                     /locus_tag="OUD09_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693699.1"
                     /translation="MIKNRLIDQVWCLDQNTDSLELIETICFNTIVLDLRAENDNCVT
                     HVIINQKMAQKLSESLRKWAKGGNDENN"
     gene            656..>3123
                     /locus_tag="OUD09_002471"
     CDS             656..>3123
                     /locus_tag="OUD09_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693700.1"
                     /translation="MKIIEKKIANKKINWLVVNFLVLIAMIIFTSNIAHASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSAVNTIVIGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSIALI
                     LLMPTMISSGIQFAKTFYKDSTTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNNLTRKNFQQTDLTQVLTDKVIDKLEKENPIADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNSGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSNGFNRAKGSGE
                     GEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERATKPFKSMRDI
                     ANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSVPTRLSDRKDGAKVEN
                     ADRIMSSSERMREAMKNNAESNNNPASAIQQKVQQDIEERKNAMHGQAKSAEELINQK
                     RHEAKYTPEAMNREEIMLRKRVEGRTGANIDGKEALTKERIQEAKNSNTGLEKLVKEN
                     PQNSTSSHGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNAQGTKTIDVRENLQSS
                     STSQPKTVDVRENVQNSTSSQGFKSVDVRENVQGSSASQPKTVDVRENVQSSTSSQGF
                     KTVDVRENIQKDSGEMKEKTQKIN"
BASE COUNT         1165 a          435 c          637 g          886 t
ORIGIN      
        1 gaattgatcg aaacatttat tgaagaaaat cttcttaaac aaatatgggg agaaagtatt
       61 gtgaattgct taaaactggc tagcaatagt gattatcgtc aattcgacaa ctggtacaaa
      121 aaattcaaat atgcgattag atctgctgaa aaagaacaaa aaattcaact gaaaattatc
      181 tatgaaattt gtaataacaa gtattttgta gatcatgtta gagaacagct atcaatgact
      241 ttacgtgact tgatacgaag agcaaaaaca gatcatcgta tcaaacaaaa agacgattat
      301 atctttgctt ctctaaaaaa taaagcgtta gaactaattg aattgcaaat cagcgaagga
      361 atagataagc aataagaata gaattgagtc aaatgaaatg gaggggtatc tccacttcat
      421 ttgagaactt tgaatatagg aggggttatt ttgattaaaa atcgtttaat agatcaagtt
      481 tggtgtctag atcaaaatac agatagtctc gaattaatcg agactatttg cttcaacact
      541 atagttctcg atttaagagc tgaaaatgat aattgtgtaa ctcatgtcat aattaatcaa
      601 aaaatggctc agaagctctc tgagagcctc agaaaatggg ctaagggagg taacgatgaa
      661 aataattgaa aaaaagattg ccaataaaaa aataaattgg ttggttgtta attttcttgt
      721 tctgatagct atgattattt ttacaagtaa tattgctcat gcgagtattt tcaattggtt
      781 tgatgacaca aaaagtcaaa cagagttttt gaataattct ctttatagac cttacctaaa
      841 aaagtacaat ggagatttcc ttagtcagtt cgggatttgg ctcggatggg ctgtggtaaa
      901 aggcatgttt actgtgacag attcgattca aaatatgatt ccagatgtgc tggatttgtt
      961 caatttcatt gaaagtacag ggctaaataa tgtttatcaa tcagccgtaa atacaattgt
     1021 cattggatta atgatccttt cattgatgtt tgttggttac aaaatgatta ctggaaaagg
     1081 aacagtggat ttaaaatcgg ttggaatgaa cgtagtgatg tctatagcat taatactttt
     1141 aatgccaacc atgatttcta gtggtattca atttgctaaa acattttata aggactctac
     1201 tacgattaca aattcagatg acggagtcgc ttggtcattg ataaaacaag gagtaacaga
     1261 tttagcatat attaataaaa cagatcagta ctcaagcatt gataaaacgg aggatcgaaa
     1321 taatttaaca cgaaaaaatt ttcaacagac ggatctcaca caagtgttaa cggataaagt
     1381 gattgataaa ttggaaaaag aaaatccaat tgcggacaat cttcgttatg aattagttga
     1441 aaattctaat aatgaatttg ttgctacaaa gttttctgac aacttcttat caacattttc
     1501 tgatagcttg aaatcaggct attaccgata ccaagcaaat ctatggggga tttcaattgg
     1561 gttgacagcg ttagcaatcg cctatgtgtt cagcgcgttt gtgatcatta cagcaatact
     1621 agaacttgca tttaaacgtg tgttaggtgt tcttgttttt gcaacagata ttgagacagg
     1681 acagcgttca aaagttgttc tatcagatat tctacaatgt tacttaactg ttgggtttca
     1741 aggatttggg ttatcaatgt ttgctatgtt tatcaatttt ctgaactcag gacaaggtat
     1801 ctcaacaaat atttttataa aaacaattgc ttatatctgt gccgtttttg ttttaatcaa
     1861 aggcagtgga acagtcatga gatactttgg tgttgatatt ggactcaaag aaggctatgg
     1921 gcaacttgct tctgcttttg gaatgggcgc aatgctgttt agaaaaggat caaatggatt
     1981 taatcgtgct aaaggaagcg gagaaggaga agatcgaaaa ccagaaaaga atttcggcga
     2041 aactctatca aaaaaagctg gcaaaactgg aagagctttg ggatatgccc atgaacgtgg
     2101 attatccggt ttagcttcag atggagctac aatggctagt gaaagagcaa caaaaccatt
     2161 taagagtatg cgtgatatag ctaatgatac caagaataag tttaaagaag gtttagatga
     2221 tggtacagtg tccgctatta ataaaaatag caaacctatg ttagctaaaa ataaagaaga
     2281 tgaaactgga aaatatgctg attccgtgcc aacacgtctt tcagatagaa aagatggtgc
     2341 taaagttgaa aatgccgata gaattatgtc tagttctgaa cgtatgcgtg aagcaatgaa
     2401 aaataatgct gaatcgaata ataatcctgc aagcgcaatt cagcaaaaag tccaacaaga
     2461 tattgaagaa cgtaaaaatg ctatgcacgg tcaagctaag tctgctgaag aattaattaa
     2521 tcagaagcgt catgaagcga aatacacgcc tgaagcaatg aatcgcgagg aaataatgtt
     2581 aaggaaacgt gtagaagggc ggacaggcgc taatattgac ggaaaagaag cgttgaccaa
     2641 agaaagaatt caagaagcta aaaattcaaa tacaggatta gaaaaattag tcaaagaaaa
     2701 tccacaaaat tctacaagta gtcatggtgg ccgatcagta gatgtccgag aaaatattca
     2761 aggttcactg aatggtcatg ttggacggaa tgttgatgta agagagaatt tacaaagttc
     2821 ttcaaatgct caaggtacta agacaattga tgttcgagaa aatctgcaaa gtagcagtac
     2881 aagtcaacca aaaacagtag atgttcgaga aaatgtacaa aattctacaa gtagtcaagg
     2941 gtttaaatca gtggatgtcc gagagaatgt gcaaggtagc agtgcaagtc aaccaaaaac
     3001 agtagatgtc cgagaaaatg tacaaagttc tacaagtagt caaggattca aaacagtaga
     3061 tgttcgggag aacattcaaa aagatagtgg agaaatgaaa gaaaaaacac aaaaaatcaa
     3121 tat
//
LOCUS       DAKBVG010000230         3096 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000230 DAKBVG010000000
VERSION     DAKBVG010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.232"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            <1..211
                     /locus_tag="OUD09_002472"
     CDS             <1..211
                     /locus_tag="OUD09_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693701.1"
                     /translation="KQTLFNLIKLDAKISSYLFFLIHDEFMDYQKLDQLIERESWEDY
                     YVGLTFSEKLNNYKLIDYKYLSESN"
     gene            828..>3096
                     /gene="esp"
                     /locus_tag="OUD09_002473"
     CDS             828..>3096
                     /gene="esp"
                     /locus_tag="OUD09_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HCT9693702.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLVGVGLVLGTT
                     ELVQAQDEISPSTPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEEPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSASAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGKKVADLAIERRKDHENYGVGSITDNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSILDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDI"
BASE COUNT         1132 a          447 c          595 g          922 t
ORIGIN      
        1 taagcaaaca ttatttaatc tgattaagct agatgctaaa atatcaagtt atttattctt
       61 tttaatacat gatgaattta tggattatca aaaattggat cagttaattg aaagagaaag
      121 ctgggaagat tattacgtgg gtctaacttt ttcagaaaag ttaaacaact acaagttaat
      181 agattataag tatttgagtg aaagcaatta attattcatg tagagtacta gacactttaa
      241 atatggacaa ttcaattata aagaaaaaga atcaataatt taatgaggct gtgacaaatg
      301 tcattttttg tttcgctaaa aacgagtgaa aaaatgttga taactccaat cattcgttta
      361 agattctagg aatcattcag gaatgctgtg aaaccgatac tttataatta taaagaaggt
      421 gatttcaaat tccgaagaag gaatttgaag tcaccttttt acttatgtca cagtctcatt
      481 cagtaatgcg ggataaggct ccttattccg ctatttgaat actctgaagt ttaatctaag
      541 aaagagtaat attttgaaaa catgcttcat cgcatagaaa tgaacttaat ttcagaaaga
      601 atacatacat atctttttat atgttttcag ctgctgaatg gttaaaaagc acaaatatcc
      661 gaaaaaaaca tgaagaaatg tacttttttt gttgtaatgt gcatgaaatt ctttaagtaa
      721 actagttaac ataagtttag taagattcct attctgtgaa taatacgtat cagatttatc
      781 ttttattctt actaagaaaa taactattaa gggagttgat ttgaataatg gttagcaaga
      841 ataataagag agtatttcta gaaaaaacaa agaaacgagt attgaagtac agtatcaaaa
      901 aattaagtgt tggggttgca tctgttttgg ttggtgttgg tcttgtcttg ggaacaactg
      961 aactagttca agcacaagat gaaatttctc caagcacccc acttgaaaca gcgataagct
     1021 ccgtacaagt aggtgacaaa gtagcttcgg ggaatacttt tcaggagaat cctggttata
     1081 cgaaaaatta taacttttcg gatttacaat ttagtcctca agaactaact ggagacacac
     1141 taaaaggcaa tacaattgga tttgaagttt acggaaaaca taatattgca gcatcaacta
     1201 aaaactggga aatccgtctt caattagatg aacgattagc taaatatgta gaaaaaattc
     1261 aggtcgatcc aaagaaagga attggatctt ctagacggac ctttgtaaga attaatgatt
     1321 cgctgggtag acctacaaat atttggaagg tcaattatat tcgtgcaagt gatggattat
     1381 ttgctggggc tgaaacaact gatacacaaa ctgctcctaa tggggtaatt acgtttgaaa
     1441 agagcttaga tgaaattttt aaagagatag gcatagacaa tctcaagacc gatcgtttaa
     1501 tgtatcgaat ctacttggta agtcatcaag acgatgataa aattgtacca ggtattgata
     1561 gtacgggcta ctttttaaca gattcagatg atttctataa tagcttagat gtatctgaaa
     1621 ataatcctga ccagtttaaa catggatcag taagtgcaaa atatgaggaa cctaatactc
     1681 aaacaaaaga tggttcagga tccacaggtg ctaatggagc tataatctta gatcataagt
     1741 taacgaaaaa ttataatttc tcatattcag cttccgcaaa gggcacgcct tggtatgcta
     1801 attacaaaat tgatgaacga ttagttccat atgtagcggg tatacagatg cacatggttc
     1861 aggctgacaa agtaacatat gatgtttctt ttgaatcagg gaaaaaagtg gctgatttag
     1921 caattgaacg gcgcaaagat catgaaaatt atggtgtagg ttcaattact gataatgatc
     1981 ttaccaaact tattgatttt gctaatgcaa gtccacgtcc agtcgttatt cgatatgttt
     2041 tacaattgac taagccatta gatgaaatct tagaagatat gaaagcaaca gcacaagttg
     2101 aagaaaataa accatttggt gaagatttca tctttgattc ttggttgtcg gatacgaata
     2161 aaaaattaat ccagaacact tatggaacag gttattatta tttgcaagat attgatggtg
     2221 atggaaaccc tgacgataaa gaagagagcg gagacacgaa tccatatatc gggaaacctg
     2281 aattagaaga agtatatgat gttgacacaa cagttaaggg gaaagtattc atccacgagt
     2341 tagcgggaac aggtcacaaa gcccaacttg ttgataaaga aggtactgta ttagcagaaa
     2401 aaactatcgc tccaaatgaa aaagatgggg ctccaatttc agatactgta gaatttgaat
     2461 ttacgggtgt agattcaagt aaattaatcg cgaaagatga attaaaaatc caaatcgttt
     2521 ctccaggttt tgataaacca gaagaaggtt caaccgttat taaggaatca ccaaaagcgg
     2581 ttgataaaca aaccgtggta gttggattta aaccagatgc taaagaatca attcggaata
     2641 ataaaaactt acctgaagat gcagagtatt catggaaaac agagcctgat acttctaacg
     2701 ttactgatag tacgaaaggt attgtaactg ttaagatcgg aaatcgaact ttcgacgtgg
     2761 atgtagagtt tgctgtaaaa gcttctcaag ctatggaaaa tgatgcaaca tacgtaccta
     2821 taacaacaac tccagaaacg acagttcaaa gtggaaaacc tacatttgat aaaccagatg
     2881 ttcctttagc taaagatgcc ttttcaattt tagatgtcta taataaggac ttcggtaatg
     2941 caagtgttga tgcaaatact ggtattgtta cattcactcc agctaaaggt gtaggagaat
     3001 cggagccgat tactggaaca attcctatta aaattgttta ccaagatggt tctgtaggca
     3061 cgaccgattt agcagtaact gtaagtaaag atattt
//
LOCUS       DAKBVG010000231         3068 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000231 DAKBVG010000000
VERSION     DAKBVG010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.233"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(334..696)
                     /locus_tag="OUD09_002474"
     CDS             complement(334..696)
                     /locus_tag="OUD09_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693703.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(770..1150)
                     /locus_tag="OUD09_002475"
     CDS             complement(770..1150)
                     /locus_tag="OUD09_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HCT9693704.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1214..2407
                     /locus_tag="OUD09_002476"
     CDS             1214..2407
                     /locus_tag="OUD09_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HCT9693705.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            complement(2484..3068)
                     /locus_tag="OUD09_002477"
     CDS             complement(2484..3068)
                     /locus_tag="OUD09_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCT9693706.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
BASE COUNT          956 a          587 c          504 g         1021 t
ORIGIN      
        1 aacgactcag ttatcttatc atgtcgttag taacatgtca ataactattt ttttaatttt
       61 ttagaaatgt gatataacat ccacttcaga atcaacgctg ttaaatttat catgtatttc
      121 ttccttggtc aactataaat taagcattac tttgtgaaat gatttttcga tcaattatta
      181 ttattcgtta tacaaccgtt ttcaacatat tttgaaaata aaatgcacta aaaaactatc
      241 tttagcagaa catttcattg tattttttgg accaaacaat aaaaaaagaa gtttgaaaaa
      301 aattcaaact tctttgttcg cttaatctgt attttagaag aaaggcactc cctctacttc
      361 gtaagttcca agaacagagc gcatttgacc tggttcttcc caaatatatc cttcgcctaa
      421 ttcttctctt tgattctttt gttcagtttc aaagacaaac aacggaacat tttcctcttt
      481 aatatgtgta tttgtcaatt ccatcaaact aatcttgcta acatcgatat gaactatttc
      541 ttttaagaaa ttaagtatat tcgctaaacc agtctttcct tcagaaagtt ccgctaacgc
      601 aaattccaac ttgtcatcaa ttgaatggac caaaaatttt ttagtaccat cctccaaatg
      661 caacataatt gtaccagcaa ctcttttgct tgccaaaatt tccacctcga tcaacttgtt
      721 ctatggctta cataaaaatt ctagcacatt ttcagaaaaa atacactttt tatttcttat
      781 tttctttccg ttctagttca gataatgctt cttctatcca aataggtaac tgctccgcaa
      841 tagtggtaaa tccgatctta cgatttttat tcgtgaaata cctttttctg cgatatggaa
      901 cagctggatt tgttttttct aacgttctta aagataaact gatttttttg gaatattcat
      961 caatgtcgat gatctgaact gttactggct gtccgacttt taacaatgaa tggatatttt
     1021 tcgtataacc ggattgtact tctgaaacat gaataagtcc ttgcgtctcg ttatcaagag
     1081 aaacgaatgc gccataaggc tgtatccctg tcacttttcc ttcaattatt tgtccaattt
     1141 tataagtcat ctacttccct ctcttcgcat ttatcattat actgtaaata gctcaaaaaa
     1201 agaagcgacg aaaatgactg gaggtcttaa gatgatcgaa tttgataaac agatttcaag
     1261 aaaagataca gaaagtgtaa aatgggacgc aattgctgag acatatcaaa tggatgactt
     1321 acttcctttg tgggtagcag atatggactt cttgtctccc ggtggggtag ctaaagcgtt
     1381 tagtgaatac atcaaacatg gaatctttgg ttatgccact ctgtctgata aactatatca
     1441 ggctatcatt cattgggaac gccaacacca tgctgtggag ttgacgaaag aaaatatcgt
     1501 ttttacaagt ggtgtgttaa ccagtttagc tgcagcagta caaactttta caaaaccagg
     1561 cgattctatt ttgattcatg acccagttta tcctccattt tcctctatta tagaaacaaa
     1621 tcaacgtcac atcgttcgaa gctctttgct agaaaaaaat aatcatttca tcatggactt
     1681 gtctgacatg gagaaaaaaa tcgaagaaaa caatgtaaaa gcaatgattt tatgcaatcc
     1741 tcacaatcct ggagggcgtg tttggagcaa agaagaattg aaacagttaa gcgaattgtg
     1801 tttaaaacat caagtgattc tgtttagtga cgagatccat caagatttaa ctttattcga
     1861 tcatacattt acttccatgc taacgattga tcctaattta gatgatctac tagttgcttt
     1921 tacttccgca accaagactt tcaatctagc tgcaatcaaa aattcaatgg tatttatcaa
     1981 aaatccagag atgaaagctg attttgaaaa acatttagtg atgaaccaac agcacgaaat
     2041 caacactttt ggactgattg gaacccaagc ggcttatgag acgggtggag aatggttaag
     2101 ccaactgatt ccttatctag aaaaaaatgt tcaaacagtt aatcagtttt ttgaacagta
     2161 tcttccgaaa gtgagaataa tgaaccctga aggaacttac ttaatgtggt tagattttac
     2221 cgcctatatt tcagacgacc aagaattgga gaacactctt gttcggaaag gaaaagtcgt
     2281 attaaatcct ggcatcacgt ttggtccttc cggtcatggg catatgcgct tgaatattgc
     2341 ttgtccagaa gaaactttac tggaaggact taatcgaatc aagtatgctt tagagacaat
     2401 cgattaaatc atttattcaa aataaaagga cgagacctta ctaggtttcg tcctttttca
     2461 taattactcc gtaccatacc aaactattct gaaatttcaa ttgtttcaat cactacatca
     2521 tgtacaggtc ggtcttgtgg accacgttgt acatttgcga tttcatctac tgtgtccatc
     2581 ccttctagta catgtccaaa gactgtatgt cggaaatcta gccaaggagt tccgccacct
     2641 ttatacgctt caatgatttc tgatgggaat ccagcacctt ctagttgtcc aagcatatta
     2701 gctggcacat ttgtgttatt tacaataaag aattggcttc cgtttgtgtt tggacctgaa
     2761 tttgccatag ataaagctcc gcgaaggttg aacagttcac gactgaattc atcttcaaaa
     2821 ctttcgccat aaatactttc gccacccatt cctgttccag ttggatcgcc gccttggatc
     2881 atgaaatcag gaataacgcg gtgaaaaatc acgccatcat aataaccttt tttggaaagt
     2941 tcaatgaaat tttgaaccgt cttcggtgct agttcaggga ataattgaac agtaatgtca
     3001 ccacggtttg ttttgataac agcttttggt cctttttcac tttttaaatc tagttgtggg
     3061 aatgccat
//
LOCUS       DAKBVG010000232         3066 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000232 DAKBVG010000000
VERSION     DAKBVG010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.234"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            187..981
                     /locus_tag="OUD09_002478"
     CDS             187..981
                     /locus_tag="OUD09_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT9693707.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSEFSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            971..3013
                     /locus_tag="OUD09_002479"
     CDS             971..3013
                     /locus_tag="OUD09_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT9693708.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKEKYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT          991 a          486 c          614 g          975 t
ORIGIN      
        1 tactatatgt agtgtatatt ttattgtgaa aaaaggtgaa aacttgtagc tggtggattt
       61 gttttttgca tatttaattt tgctgaataa aataattgaa taaaattttt tattcagtcc
      121 aagtaaaagt gacaaaaaca taagagcaaa ttattcgtag atatgctact atgcaaataa
      181 atagaaatga aaaatgaaag aggaggagaa gaattggaaa agatgatcga agtgaaacat
      241 ctaagtaaaa gctacggcag caaactttct cccgtcaaag ccttagataa tatctcattc
      301 acagtagaag aaggcgagtt tgttgggatt atgggcccaa gtggtgcagg aaagacaacg
      361 ctgatgaata tcttgtccac gatcaatttg cctacgatgg gcagtgtctc gatccaagga
      421 aaagaaatca caaaaatgaa aaacagtgag ttcagtgact ttcgtcgaaa aaaactagga
      481 ttcatttttc aagagttcaa tttgatcgat accctaaatg caaaagacaa tatcctattg
      541 cccctagcag ttgaacggat gactaaagag gaaatggaaa agagagtaag acatgtagct
      601 caattgttga atatagaaga actgctcaaa cggtatcccg atgaactttc tgtcggacag
      661 agacaacgaa ttgcagctgc tagagcactt gttgttcagc cacaagtgat ttttgcagat
      721 gaaccaactg gctctttgga ctcgaagtcg gctacagaat tgttgaacta cttgaagacc
      781 atgaatcaaa aagaaaaagc aacgatactg ttagtcaccc atgatccata tacagcaagc
      841 tactgcgata gaattttatt tattaaagat ggtgtgattt tttctgaagt tgtcagaaga
      901 gggacaagaa gagagttctt tgagaaagta atagacatgc aggcgacgat tggcggagga
      961 ggaaaaatga atgctgtata aactagccgt taaaagtttt tttcggcagt cacgaggcta
     1021 tcttgtttat tttttcagtt tgactttatc cgtaatgatc tattattcct tcagcgcaat
     1081 gacctacgat caatctttag tcagaagagc aagtcaagat acttcgatag atggtggatt
     1141 gagttttggt ggatttatca ttactgttgt tttgcttttt ttcgtattca gtgccaaccg
     1201 atttttcttg aatagaaggc aaaaggaaat aggcatctat cagctttttg gtatgaataa
     1261 actgcagata tcaggtatat atgtgttaga aatcatgata attggattgt tcgcctgtat
     1321 ttctgggata ttactcggta ttattttttc caaactcttt tcaatgattt tggttcgaat
     1381 gatggatatg gatcttacta gtccattttt tatctccatt ccatcagtag tagatacttt
     1441 atttgctttt ttcatcatcc ttttagcagt atctgtatat tcaatatgga aaatatggcg
     1501 ctatccaatg attcgaagtt ttggagaaaa agaacaagca gatagttcaa caatgcgctt
     1561 caggacaagg caccgattac tcgcagtcat cgggttacta cttttaacga gcggatattt
     1621 tggagctagt cattttaggg aaatcacttc atggatgatc agttatcatt atcttgatac
     1681 attagttttc ttttttcctt ttttaatttt tttcgtttgt gtggtcggta cttatttgtt
     1741 tttctgttat acatttcggt tatttttgat catattgagt aaatggaaga tttattacta
     1801 tggtatcaat tttttgataa tgggtaacac gcaagttcat ttgctcaaaa gttggcgtac
     1861 aaattctctg attacagtca ttctcggaat ttctttaatg acaattggcg gcacggctag
     1921 tatctctact attttatcct accaggagaa agtttcctcc ccgatggact atcagtttga
     1981 tgttgagaca gagaaaaaaa tcaagccgat tctagcagag gaagggcaga agataactaa
     2041 agaaatcacc ttgcattata aagtcattgg gagtatctat agtcaaaatc tcgaagaacc
     2101 tatgtttcaa actgctaatc tgatcacgga aacacaatat caagccttca gaaaagttaa
     2161 tccagatttg ccgaacattt ctttgaaatc acctggtcat actgttttgc ttgatcaaat
     2221 gcaaactctt tttcggaata tttcattata tggatctagt attggactgc cagataatca
     2281 agaattgaag attcaacaaa tgcttccaag tgtattaggt gacgaaacaa tggtatatct
     2341 aagtcctgta ctgattgtag atgataccgt ttttgaaaaa gcaaaaggtg tggattatca
     2401 agtagtcaat gtagatgtat caggtggaaa taatgaaaaa atagatgaac gactgagcaa
     2461 tgacctgtcg ataaagtggc tcaatgctgt ttattattct tatgatatcg taaatggaag
     2521 tctacaagga gaaattagca caaagaaact tccggatgac caaatgaagg aagcacaatt
     2581 tagccaagaa agctctcgtt tgaatttctc ttccagctat tcgaaattgc gtgcagttaa
     2641 tcggcgaatc ggtatcaata tttttgtcac actatttgtc gggatgatcg tgattattgc
     2701 tactggtagt attttgacag tccggcaatt agcagaagca gaggaagaaa aagaaaaata
     2761 tcacctattg actaagctgg ggattccaca aagaaggatc agacggatga tcttcgagca
     2821 aaacgcactt accttctttc cacccatgat attagggatc actcacgcag tatttgcaat
     2881 caatgtcttc actcagtata taaaaactgc cgattactgg ttggcttatc ttgtgagtgg
     2941 cttattgatc cttatctatc ttttgcttta tgtcattacg agtcagttat attgtcggat
     3001 tatcgaggaa tgatatacgg aataagacaa gataataaat agaactaaat tttgaatacc
     3061 atttat
//
LOCUS       DAKBVG010000233         3064 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000233 DAKBVG010000000
VERSION     DAKBVG010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3064
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.235"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(165..1112)
                     /locus_tag="OUD09_002480"
     CDS             complement(165..1112)
                     /locus_tag="OUD09_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCT9693709.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDVDSVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            1357..1821
                     /locus_tag="OUD09_002481"
     CDS             1357..1821
                     /locus_tag="OUD09_002481"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCT9693710.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQQILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(1883..2926)
                     /locus_tag="OUD09_002482"
     CDS             complement(1883..2926)
                     /locus_tag="OUD09_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HCT9693711.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGIKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
BASE COUNT         1010 a          605 c          587 g          862 t
ORIGIN      
        1 aagaaggaat ttacgaaaat tgcttctcaa atttttgtga aatttcggct tatttccgaa
       61 gaagctactt ctggaacacc atttattcgg aaaaaaggac tgtgacaaga cttttgtcac
      121 agtccttttt catttaaaaa aatgtttact tcatttgacg tatattattg gtgaggcttc
      181 aattcatttg gcttcacttt ttctgtttct cttatgatat aaacaggacg ctgtttcgtt
      241 tctaagaaga ttttcccgat atatttcccg atgattccaa gacataacaa ttggattcca
      301 ccaatgaaca ggaagatcga gaccatggaa ggccatcctg atgtaggatc accaaacaac
      361 aatgcccgca aaataatgaa caaaattgca ataccagaac cgacacaaga caaagcgccg
      421 ataaatgaag caatcgttaa cggtgcatcc gagaagttca cgataccatc aatcgagtag
      481 ttgaataatt tccagaagga ccaagatgtt tcgccagcca cacgttcgcg gttttcataa
      541 gaaatatatt ctgttttaaa tccgacccag ctaaataatc cttttgaaaa tcggttgtat
      601 tctgttaatt ctaggacggc atccaccatt tgacgagtca tcaagcggaa atcacgagcg
      661 ccatcgacca tttctgtatc gctgatttta ttgatcaact ggtagaattt tttcgcaaag
      721 aaactgcgta tcgcaggctc gccttcacga gtcgaacgcc gggtgccgac actatcgaca
      781 tcagaagtag tgatccggtt gatcatctca ggaagcagtt cgggcggatc ttgcagatca
      841 acatccatca cggtcactaa atctcctgta gaagcttgca atccagcgta cagagccgct
      901 tctttcccaa aatttctgga aaatgagaca aagcggacat aaggataagc tgaggctagc
      961 ttcttgattg ctgctaacgt accgtctttt gatccgtcgt caataaacaa atattcgaac
     1021 tcatgggcac ctttgaattt ttcaacctca tcaaaaaaaa gaggaacagt tttttcttca
     1081 ttaaaacatg ggacaacaat tgatactttc atcgtttcta tcctttcaat cgtatgttta
     1141 tatatggttt acattcaaaa tttaataaac cttaaataac accttttaaa tttgatttta
     1201 tggaaaagaa cagctattct ttggtaaact aacgtattta gtttaccatg tttcctcggt
     1261 ttatcaaact tttaagaaca atttttatta gttggcaagt agttttggat agagaaaaag
     1321 gaggaagttc cagtctactg accaggagca aacaaaatga aaatacacac acctatcggc
     1381 tttttatggt tagatgcaga tgaatccgga ttgactgctg tatcttttca gccaatttca
     1441 gaaagcgaat cagcgaatca gcaaatttta acgcgcacac aagaacagtt tgaagaatat
     1501 tttgcgggaa aaagaaaaga atttgatgtt cctttatcta taaagagagg tacgcctttt
     1561 cagcaaaatg tctggaaagc tttgtcgacc attccttatg gagaaatccg aacgtataaa
     1621 gaagtagctg ttgctgttga tagtccgaaa gctgtgcgag cgattgggca ggcaaatcga
     1681 accaatccat tgccaatcgt tattccttgt catcgagtga tcgggcaaaa tggaaaactg
     1741 acaggatata tgggaaacgc aatagatggg gttgaaatca aacgccaatt gttgttgtta
     1801 gaaggatatc tgcaaaatta aaaacagttg tgattcggac attgttccat gcgagaatca
     1861 caactgtttt ttcatgttca ttctaaactt tgatcagcca aataaatagt aaagccatca
     1921 ctgtttggag aagcccgaca gctaatccat aagataagaa gcgtgtacct tctttcatga
     1981 attttgcaaa gtctaaacgc agaccaattg ccgctaaagc agtgatctca aaccagctgc
     2041 tgataaaatg tgccgattgg tcaataacta caggtaaaga aaacaagctg ttgaccaaac
     2101 aaaagatcac aaaccctaat acataccaag gaagtggtaa ttttcgtttt ttctgcgtat
     2161 cagttggcgt ttcaaacaga gctggttgtt tcttgtgcat gaatttttca aacagataga
     2221 caacaacaac gagcatcata atacgtagaa tcttaaatag catggcgaat tcgacaacag
     2281 aattgtcaag taagcttgcc ccagcgacta cttgaccaac agattggagt gtgccaccca
     2341 aaagtgcact tttttcaatg agatttccac caaacaactg taatccaagg aaagggagag
     2401 tcaacatcat cacagtaccc agtagattga ctagtgtgat gatctgtcct ttttcttcct
     2461 ctttcgcatg gatggaaggg gcgatcgaag caatagcaga agaaccacaa acagcgtttc
     2521 ctccagccat catcaaagaa gaagtatcgc tgaattgcag ttttttacca atcaagtaag
     2581 caccgatgat agtgactgac atttgcagta acacaaaaac aattcctttt ataccaattt
     2641 gagcaatcgt ttgagctgtg acagttgcac ctagtaaaaa aacagagaat tctaatagtt
     2701 tgctttcagc aaattttgtg ccgattgcta gaacaggctg tttaaaaaaa gtatttccta
     2761 ataaaatacc caataaaata gcaatagtcg ctccacctaa ctgtggcaga taaatagcaa
     2821 gaagcctgct gaaggcagcg atggcaaaag aaagcagtaa accgggtaag atttcgcgga
     2881 ccgctgaatc ctttacagat tttattggat gtgacaatga tttcatttga tcggttcctt
     2941 tcgtcaataa atactaaaag catactcgaa catagtagca ctaaaccagt attttgacaa
     3001 aagaacaaga ggtcgtgaca aaagtcttgt cacgacctct tgtttcgaat aaacggtgtt
     3061 caaa
//
LOCUS       DAKBVG010000234         3063 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000234 DAKBVG010000000
VERSION     DAKBVG010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.236"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(89..1618)
                     /gene="ahpF"
                     /locus_tag="OUD09_002483"
     CDS             complement(89..1618)
                     /gene="ahpF"
                     /locus_tag="OUD09_002483"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCT9693712.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(1628..2191)
                     /gene="ahpC"
                     /locus_tag="OUD09_002484"
     CDS             complement(1628..2191)
                     /gene="ahpC"
                     /locus_tag="OUD09_002484"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HCT9693713.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(2335..2997)
                     /locus_tag="OUD09_002485"
     CDS             complement(2335..2997)
                     /locus_tag="OUD09_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCT9693714.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
BASE COUNT          896 a          633 c          531 g         1003 t
ORIGIN      
        1 cgattattca aaaaaacaaa aaggattgtg acaaaattct tgtcaccatc cttttttccg
       61 ctctctgact ttttacaatc ttcatagctc aagtgcgcat cagataatcg aatgctccta
      121 aagctgcagt agctcctgaa cccattgaaa caatgatttg tttgtatgga ctatctgtac
      181 aatcaccagc tgcgaaaacg ccggggatat tcgttgcgcc atttttctcg gtcactattt
      241 catttcttgg attcatttcg atcgttccat ctagccaatc cgtattaggg agtaagccga
      301 ctagaatgaa tactcctgaa acagctagtg tatgcatctc catcgtacgg cgatcgatat
      361 aggagagaga ttctacctga ttcgtaccgc tgatttcttt cgtcgctgca tgtttgataa
      421 cagtgacatt cgcaagagag actagtttat cctgtaagat ttgatctgct tttaattctg
      481 gtaagaattc tagaacatat acatgttttg ctaacccagc caaatcgatt gctgcttcaa
      541 caccagaatt accaccaccg ataacagcaa tctcttttcc tttaaataaa gggccgtcac
      601 agtgtgggca ataagcaatt cctttatttt taaactcttt ttcgcctggt acattgatag
      661 aacgccagtg cgcacctgtc gcgatgatgg cggtttttgc tttaagtgat ataccgttag
      721 ctaagccaat attaataaaa tgttgctctt tatttaagga aacggcctgt tgattcatca
      781 taatgtcgat agggtaacta cgcacatgac tttctacttg ctgcatcaat tttggacctt
      841 ctgtgtaaga ggtaccaatc atattttcaa tccctagtgt ttcaacgact tgtccgccga
      901 aagaatcagc gacaagtcct gtacggattc ctttacgtgc tgcatagata gctgcactac
      961 tagctgctgg tccaccgcca atcactaata catcataagg ttctttttca aacaactctt
     1021 cttcaatcaa agggccagtg attttttcaa caatttgttc gatcgtcatg cgtccgctac
     1081 taaattcttc tccatttaaa aacacagttg gcactgccat gattttttta tcatcgattt
     1141 ctttttggaa catcccacct tcaatcatcg tatgagtaat atttggattt aatacagcta
     1201 atatattcaa tgcctgtaca acgtcggggc aattatgaca agtcaagcta acataactgt
     1261 caaaatgcaa tgtttgtgtt atttttttga ttttaagact caaagattca ctgattttcg
     1321 gtgcccgtcc actaatttgt aacatagcca gtaaaaaaga ggaaaattca tgaccgaggg
     1381 ggataccagc aaaaacgatt ccactttctt tgccaactgg attgatagaa aaactaggtg
     1441 tgcgcatcga atctcttttt tcccaagaga ttttaggaga catttctgct atttccttcg
     1501 taaattcagc tagtttttga ctattttcac tattatccaa actaacagaa aaaacaatag
     1561 gagattcaat cagtgctaaa tattgttcga gattttgttt agtttctttg tctaacatat
     1621 ccaccaccta aatctttcca acaagatcaa aacttggttt caatgtttcg ccactttctt
     1681 tccattttgc cggacaaact tcacctgggt gggtacgtac atattgagca gcgcgaatct
     1741 tatcaatcag tgcactagcg tcacgaccga ttccatctgc attgatttcc atagcttgaa
     1801 cgattccgtc tggatcaata ataaatgttc ctcgctgagc taatccttgc tcttcatcca
     1861 aaacatcaaa aatacgagaa atctgatgag acggatcacc gatcatcacg tattcgattt
     1921 taccgatagc atctgatgta tcatgccatg ctttgtgagt aaagtgggta tcggtagagc
     1981 aagaaaagac ttcaacacct aaagatttca atgtactgta ttgttcttgt aagtcttcta
     2041 attcagtagg acaaacaaaa gtaaaatcag ctggatagaa gcaaagaata ctccagttcc
     2101 ccataagatc tttttcactg acttctaaaa attctccttg atgataagca tgtgcattaa
     2161 attcttcgat ttttttccca atcaatgaca tagtaaaacc tccaataata attatttatt
     2221 tataattatt gtaatttagt tggagttgca aagtcaatgg atattttgtt atttaataaa
     2281 gattactcaa aaacgaaggt taattataaa aatagaagag gtcgcaagaa aatgctactg
     2341 ctggaaatac tcgggataac gttgagtaac agtttcctgt tctttcagca tcatgtgtaa
     2401 gtgcaatccc gtttgaaatc gagcttcttc tggtttttta tcaactaaag ccttgagtaa
     2461 atcataatgt tgttccatga tcgtttccca tggaagcccc tgaatggtca ctcgtaacca
     2521 gcggaaacgt tctaaatggg tattatggtc atctaaccag ttccaaactt cttttcttcc
     2581 tgcaatttcg aaacaagctt catgaaataa attatctgca cggaaaaaag cgcgttcgtc
     2641 atttattttg actgcttttt cttgttcttc taaaatcgtt tctagcacta ttcgtgtttg
     2701 tgtgtccaat ttggaacaac attcaatcat gatttgctct tccaactgtt ctcggacaaa
     2761 gcgagcattt tctgcagaaa caagattaat ttttgaaaca tacgtaccac tttgaggtat
     2821 cgtatacacg agttcttgct gacgtagttg aatcaatgct tcgcgaatgg gagtccggcc
     2881 gatagcaaga tgttcctcca gtgccttttc agatattttc cttccaggtt ctaagtcagc
     2941 atagataatt tgtttacgaa tcgcttgata agcttgagat tgcaagctgc ttttcatctt
     3001 tattcctcca aaatcatttc tatgaacgta gtataacaga agaggtctga aaaacaatct
     3061 ggt
//
LOCUS       DAKBVG010000235         3053 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000235 DAKBVG010000000
VERSION     DAKBVG010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3053
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.237"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(<1..135)
                     /locus_tag="OUD09_002486"
     CDS             complement(<1..135)
                     /locus_tag="OUD09_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCT9693715.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     I"
     gene            583..2946
                     /locus_tag="OUD09_002487"
     CDS             583..2946
                     /locus_tag="OUD09_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HCT9693716.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKLGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRNDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFHSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAATLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
BASE COUNT         1049 a          554 c          639 g          811 t
ORIGIN      
        1 gataccgata acggaagcta gattgtaatc tattttagtg acagtcaggt atagccaagt
       61 aactagaaga tcagtggaac ctgcaccata aaagtttgag tttgtcggtc cgttgacagt
      121 taaaagaaaa atcacgttga agttattgat atttccgatt acgccgcttc gatttggtca
      181 cgatccaaat tttggataat ccctgtagaa attagcatag tagctgggat accaattcac
      241 atattgacaa agataacaga gacttttaca taaaacggat caatcaggaa aggaattgct
      301 cgatccagcc ccagttttcc aacaatgtgt taactggcct agcgtcattc agcaaattat
      361 gcatgatgag gagcgaaatg aactgcggaa cagccattgt gataacgaca agttttttgg
      421 atcaatgtaa aaaacgtcag cgaaaggaaa aatttcgtgg ttttttatac ctgttcattt
      481 ggctagatat aaaatccaaa caaatgaaca gaagtgctta ttaggtttca attgggagta
      541 tctgcgctag aataagagta ataatgaaaa ggagtgttat gtatggatta ttctagtaaa
      601 gaatactttg ataaaatgac ggcttggtgg cgtgctgcta attatctttc tgttgggcaa
      661 ctttatctta aagataaccc cttactgaga cgtacactga aaccagaaga tgtcaaaaaa
      721 catccaatcg gtcattgggg aacgattcct ggccaaaatt ttatttatgt ccatttgaac
      781 cgagtcatca ataaatatga tttgaacatg ttttatatcg aagggccagg acacggcgga
      841 caagtcatgg tatcgaatgc ttatttagat ggaagttaca cagagatcta tccagaagta
      901 acggaagatg agacaggaat gcaaaaatta ttcaagcgat tttcatttcc tggaggcatt
      961 gcttctcatg cagcaccaga aacaccaggt tctatccatg aaggcggaga gcttggatac
     1021 tctctttcac atgcagtcgg agctgtatta gacaatcctg aagtaatctc tgctgtcgtg
     1081 attggtgatg gtgaagcaga aaccggtcct ttagctggaa gctggttctc aaatgtcttt
     1141 atcaatccag tgatagatgg agctgtttta ccgatccttc atttaaatgg tgcaaaaata
     1201 gccaacccaa cgatcttggc acgtaaatca gatggagaat tagcaaatta ctttaacgga
     1261 cttggctggg agcctttctt tattgaagga aatgatccag aaaaattaaa tcctgttatg
     1321 gctgaaaaaa tggatcaagc aatcgagaaa atcaaatcca tccaaaaaga agctcgcctg
     1381 aagacagcaa cagatgtcgt gatgccaaaa tggccagtat tgatcgtacg cacaccaaaa
     1441 ggatggaccg gtccaaaaga atgggacggg gaaccaattg aaggaacctt ccgagcacac
     1501 caagtaccga ttcctgtaga tcaagaacat atggatcatg cagatgcttt actccgttgg
     1561 ctgaaatcct atgaaccaga aaaattattt gatgcacaag gacgtatttt agaagaaatc
     1621 agagaaattg cgcctactgg tgaccaaaga atggcaaaaa atcctatcac caatggtgga
     1681 atcgatccaa gaccgcttat tatgccagat tggaaaaaat atactttgca atttgaaaaa
     1741 ctaggctccg ttaaagcaga agatatgacc gaacttggta aattcgttcg cgagatcatt
     1801 gagaaaaatc cagaaaattt ccgtatattt ggaccagatg aaacaaaatc aaaccgattg
     1861 aatcaagttt tcaaaacgac aaatcgccaa tggatggaaa aaatcgaacc agaaaatgat
     1921 gaatggcttt cgccatctgg tcgcgtcatc gacagtcaat tatcggaaca ccaagatgaa
     1981 gggttcttag aagggtacgt tctgactgga cggcatggtt tttttgcaag ttacgaatca
     2041 ttcttgcgag tggttgactc tatgttgact cagcacttca aatggatacg gaaatcacat
     2101 gacctttctt ggcgaaatga ttatccttcg ctaaatctca ttgcaagctc aacagtcttc
     2161 cagcaagatc acaatggata tagccaccaa gatccaggaa tattgacaca tttggcagaa
     2221 aagaaggcgg agttcatccg tgaatatctg cctgcggatg caaatacatt gctcgcagtc
     2281 atggataaag cattccactc gtcagaaaaa atcaatttga tcatctcttc caaacatccg
     2341 cgtgctcaat tttactcagc agaagaagca gccgtcttag tgaatgaagg cttgaaaatc
     2401 attgattggg cttcgacagc aaaagaggag gaacctgaat tggtcattgc tgctgcagga
     2461 acggaatcta acttagaagc attagcagct gcaactttgc tgctggaaga attcccaaaa
     2521 ctaaaaattc ggttcattaa tgtcgtagat ttattgaaat tacgtcatcc aagtcaagat
     2581 ccgcgtgggt tgagcgatga agaatttgat caatatttta caaaagacaa accgatctta
     2641 tttgctttcc acggttatga aacattggtc cgcacgattt tctttgatcg tcataaccat
     2701 catttaatga ttcatggcta caaagaaaat ggagatatca cgacaccatt tgacatgcgt
     2761 gtagtaaatg aacttgatcg ttatcatttg gcaaaagacg cagctttaaa aatcaaagga
     2821 agtcaagcag aagattttgc ggaaaaaatg gatcagaaat tacaagaaca tcaaaactat
     2881 attcgtgaaa atggtattga tctgccagaa gtattggatt ggaaatggaa aaatcttgat
     2941 caataaaatg ataaaatcga tcccgcaaaa cagaattttt tacattgatt aagaaactga
     3001 aaaaataaat aaaaaaaacg gaaaaaagac tggaagaatg gacagtcttt ttt
//
LOCUS       DAKBVG010000236         3021 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000236 DAKBVG010000000
VERSION     DAKBVG010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3021
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.238"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(9..944)
                     /gene="murB"
                     /locus_tag="OUD09_002488"
     CDS             complement(9..944)
                     /gene="murB"
                     /locus_tag="OUD09_002488"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCT9693717.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1017..1937)
                     /locus_tag="OUD09_002489"
     CDS             complement(1017..1937)
                     /locus_tag="OUD09_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCT9693718.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2050..2802
                     /gene="xth"
                     /locus_tag="OUD09_002490"
     CDS             2050..2802
                     /gene="xth"
                     /locus_tag="OUD09_002490"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCT9693719.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
BASE COUNT          852 a          608 c          525 g         1036 t
ORIGIN      
        1 attacttatt aattggctaa acttctgctt gtttctgata ttgcttgttt ttctccaatg
       61 atccgcactt ctgtttctaa agacacatca aatttttctt tgattacttc ttggatgtgc
      121 gcaatcaatt caatataatc tgtcgctgtc gcatgatcga tattgacgat gaatccagca
      181 tgtttttcag agacttgtgc accgccccat ttcaggcctt gcaatcctgc ttgttggatc
      241 agctgacctg tatagtgtcc tactgggcgc ttaaagacac ttccgcatga agggtattct
      301 aatggctgtt ttgattgacg caattcggtc aactcatcca ttcttgcttt gattgcttca
      361 taatcccctg tttgcaaact gaatcgagct tccaatacga tagcacgtaa ttcttggact
      421 ttactgtgac gataagaaaa taccatttcg tcttttgtca aagttttcaa tgttccatct
      481 tcaagcaaaa gatccacttc agcaaaaaca tctttgatct ctccgccata cgcacctgca
      541 ttcatataaa ccgctccacc gacacttccc gggatgccgc aagcaaattc aaatccagtc
      601 aatgattctg ccaaagctgc ataagtcgta tcaatcagtg aggcacctgc ttccgcaatg
      661 accatcgttc ctttgacatg aatctgtttc atttcagtca gcatgatgac tacaccgcga
      721 atcccgccat cttgaacgat cagattgctt gcatttccta ataccatcca aggtatatca
      781 tggattcggc aataatcgat gatttgcttt acttcttctt gttttttcgg gaatgcaagg
      841 acatcagctg gtccacctgt tttcgtaaac gtatagttca tcaatggttc atcaaacaat
      901 atagtaatcg tggctaagct ttcaactatt tcttctttat tcactgagga ttttcctttc
      961 ttttctcaaa cgtcctcatt attctatcac gcttaataca atcttgccag tcttgttcaa
     1021 agttgatgac ggtagtgatc tttaagcact tcctcacata attcatgact tcttacaact
     1081 tctttttgtt taagcaattc tgtttccgat ttacctaaac ggatctgaga aaagaaggcc
     1141 tctaccattg gctcaaaccc gcgtttgact agcgtgtttg tccagtcgcc aaattctttc
     1201 actttttttc catcagcatc ttgaatgaca agctgggtca gatcgtttac ttcataagta
     1261 ccatttttac ttgttacttg atatttttct atattagcgc cacttgctaa atccatcgtt
     1321 aaaatagccg tttgattttg tgtttctaat tgtaaaatgg catattccaa aaattccttt
     1381 gtttctctta tctggtattt tgttctcaaa atgggttcat ctaataaata cacagctgta
     1441 tcaataagat gtaaaaacaa atcataaagg acgaactctg tcgtttcttt tgccgctatt
     1501 cgattttttt gtaggtggat caaccgctta tctgggatac gtttcaattc ttctaccata
     1561 ggagcaaagc gccggttgaa accgatcatc aaaattattt ggttcttatc agctaattct
     1621 tgcaattcac gaacttcatt tagatggaca ctcagtggtt tgtcaatata aacatggata
     1681 ccattttcta gacatttttt ggctaattca taatgtactt ttgttgctgc gtggatcata
     1741 catgcttcga ttttttcttc taaaagctca tcgatcgttt catatagatg ttggaacccg
     1801 tactcttttt gaattctttc ttttgtttct ttatttcttg ttgcaaaata aaaatcaagt
     1861 gttccttgtt ttttcgcata agttggtaaa tatgcttttt ctgcgatatt ccctacaccg
     1921 attacaccaa tcttcatcta ctttcctcct ggatcattta gttactttca tcttacacca
     1981 ttttttacta aaatgaggta caatagtatc gcaaaggtaa ggaaattaat tcgaaggagt
     2041 gtgcttttat tgaaatttat ctcttggaac gtaaacgggc ttcgtgccat cgtaaataaa
     2101 aattttttag aagtttttca tgagttagac gccgattttt tctgtctgca ggaaacgaaa
     2161 ctccaagcag gacaaattga tttagatctt ccaggttatt atcaatattg gaattacgcg
     2221 gaacgaaaag gctattccgg cacagcgatt tttgctaaaa aaccagcatt gaatgccact
     2281 tacggaatgg gtatcgatat ccatgataca gaaggccgtc tgattacttt ggaatattcc
     2341 gatttctttc ttgtaacctg ttacacgcca aattctcaaa gcgaattaaa acgtttagat
     2401 taccgattag aatgggaaga agccttttat aattatttgg aaaatctaaa aaaacaaaaa
     2461 ccagtcatcg tgtgtgggga tctaaatgtc gctcatcaaa agattgattt gaaaaactgg
     2521 aaaacgaatc aaaaaaatgc tggattcact ccagaagaaa gagcggctct ttctcgactg
     2581 ttggataacg ggtttatcga tacattccgt tatttttatc caacccagga aggtgtctac
     2641 tcttggtgga actaccgatt caattcaagg aaaaacaatg ctggttggcg gatcgattac
     2701 tttttgacga gcaaagattt agagccagga ctagctgatg caaagatcca tacggacatt
     2761 atgggcagtg atcattgtcc agtagaatta gatttgaaat aaagaggttg tgaaaaagtc
     2821 gtttagtttc gagtattaag aaaaattttg tgaaaatggc tttccacatt ttgcataaat
     2881 ttggcataat accgagaaac tggcttttga acactgttta tcgagagatt gtgaaaaagc
     2941 tgtcctgcat caagtaataa gaacagccaa acaaaaatag ttcctcatat ttttcgtttg
     3001 gttgggctta ttaccgcaga t
//
LOCUS       DAKBVG010000237         2828 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000237 DAKBVG010000000
VERSION     DAKBVG010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.239"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(<1..199)
                     /locus_tag="OUD09_002491"
     CDS             complement(<1..199)
                     /locus_tag="OUD09_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCT9693720.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSL"
     gene            complement(199..429)
                     /locus_tag="OUD09_002492"
     CDS             complement(199..429)
                     /locus_tag="OUD09_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCT9693721.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            725..1063
                     /locus_tag="OUD09_002493"
     CDS             725..1063
                     /locus_tag="OUD09_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693722.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPIQIET
                     LVQRISTREDFAIVECEIVAIFVEEGILHDPTHINVDKWQPLIYKLREYTTANQSLSL
                     NFRFQEFKES"
     gene            complement(1268..1843)
                     /locus_tag="OUD09_002494"
     CDS             complement(1268..1843)
                     /locus_tag="OUD09_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HCT9693723.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPEQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDVSFARKIITQQMPELVMEKV"
     gene            complement(2208..2789)
                     /locus_tag="OUD09_002495"
     CDS             complement(2208..2789)
                     /locus_tag="OUD09_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCT9693724.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASETGKGQFYYYFS
                     SKKEVCLAVIHAHMVVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWIKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
BASE COUNT          862 a          573 c          448 g          945 t
ORIGIN      
        1 gtaatgagta acgtgtcggt aaattcttta tagtggaagt aattggacat atgaccgaaa
       61 acattgtttt acggttaaat tcataacgag agacgactag tcctggtcgt cttttttgaa
      121 tttcttttcc tactgaggga tcaaaatcaa tccatacaat atcacctttt ttaggaatat
      181 aaattctttc ttctgacact ataaaatttc tctcccttca ggagttaaat cttcccattc
      241 gtctttctca taatactcgg cctcttcccc accactaaat ggatcttcaa tttttggaac
      301 taacgtaatt gttccatcat cagaatacac aacgatatat tcttggtttt ctgaaggttt
      361 ctttccgtta tcagaaggta aagttaccac tacagaactt ccttgtaatc tagattttgt
      421 aacaagcatt atcatcactc ccttaataaa agtatactat ataacactaa gtaacgcaaa
      481 attccccttt gtaaactatc tagaaaattg ttttgtttaa agtatatctt attggatata
      541 aatagaaata aattgtagcc agttcatttt ttctttaaat aaaaaaataa tccaagaaac
      601 attgaaccta tggatccaaa agctacccaa atactatagc taactccccg ctaccttcaa
      661 tcttgcagat aaaacgcttt ggaaaaaaat cgagaaaatc gcaaagacga caggccatcc
      721 tatcatgcct gattataaac aaaaagctgg ctattccttt tgctctgaca aatttcaatt
      781 gggaaagttc acgaaactag caggtgaaga agtttcaact gttcggatta aagaatgccc
      841 gattcaaata gaaacgttgg ttcaacgaat ttcaactaga gaagactttg ctattgtaga
      901 atgtgagatc gtagctattt ttgtagaaga agggatttta catgatccaa ctcatatcaa
      961 tgtggataaa tggcaacctc ttatctataa actcagagaa tatacaactg ccaaccagtc
     1021 gttaagtttg aattttagat tccaagaatt caaagagtca taatgataaa catcattagg
     1081 tataaataaa aaagactcgc agaacgagta aaaaacaaat agaagctata ccttagaaag
     1141 gtcgactgtg actctataga tgcagtatat gcagcttatg agcatttctt ctgttctctt
     1201 tgtttttggg atctgtgctg aagtcttttt cgttttttgc cgctctttct ttgaaaaatc
     1261 gattatgtta taccttctcc attactaatt caggcatctg ttgagtaatg atttttcgcg
     1321 caaaagaaac atctgttacc caaggttcga tttgttcttc agtcaagaca agcggcattc
     1381 ggtcatggat tggtgcgact gtgcggttag gttcagtagt cattatgatt gattctgcca
     1441 ttttttcatc ctgatggcta cgataaaaac cgccaatata cactattttt tccttatttg
     1501 aaaacataaa tttgtctttt tcttcattcc actcataaaa gccactcatt gggaaaacac
     1561 aacgattttg caaaaatggt tgatgaaagg tctttttttc ttctactgtc tctgcacggg
     1621 cattaatcat caattgtcct tttttaaatc cggtaaatcc ccacttcatg atttccggag
     1681 tgaggatgtt ttcttcctct tttcttaacg tcacaacaag atttgacgga aaaacttctc
     1741 cagctgctaa ttctgctgcc tgctccggtt gtttcgtctg ggcttctcga taataattct
     1801 gaaggagctc tgactctaga tcaaaaaaat aacgaccaca catcctaatc cctcccaatc
     1861 tttttttcta tcatacactg caacaagaag tccgtaccaa caaaacgctt tcaatttgtg
     1921 tagaactaaa aaagttgtga caagtgacaa actctccagt gaatggtatt ctcctgccaa
     1981 ctcctcggtc acaagtcaca atattcaaca aacatgagaa gctgcttttt gaaattgttc
     2041 cttgtgactc ggagtgaaac ggcaggctta cgacctctgg gtaaacggtg ttccagaagt
     2101 agcttcttcg aaaaataagc cgaaatttca caaaaatttg agaagcaatt ttcgtaaatt
     2161 ccttcttatt tcttgaagct gaccacttct gtcacaacct ctaattatta aagaaccttt
     2221 tgcgaagtta acaatctttc tttgatcaat tgaaaatttt gtctaagtag tgatagatcc
     2281 tgtgtgactt tcaacaataa gatacttccc tggatttcag caatcaacgc tttggcttga
     2341 cggtctgctt cttcatcgga cagtttttct tcagaaagtg ccaggatatt ttgtttcgta
     2401 tacttgatcc attgattgac taattctagt agcaacagac ggtattcttc atccactgta
     2461 ctcaactcaa taatcaaatt acctacagga cagccatgat aagtgatctg tgtctcatga
     2521 tcagcaaaaa tccagtccaa catttcttct aatgcttctt ctggctgttt tccagcattt
     2581 aagatgtctt cgaacaattc tgtctgccac acgaccatat gcgcatggat caccgctaga
     2641 caaacttctt tttttgaaga aaagtaataa taaaattgcc ctttccccgt ctcagaagct
     2701 gctagaatat ctcggattga cgtttcatga tagccttttt tataaataag atctttggtc
     2761 gcttccaaga tcttttcctt tgttttcaat gatattctcc tttttcattt ctcatagtcc
     2821 tatattat
//
LOCUS       DAKBVG010000238         2770 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000238 DAKBVG010000000
VERSION     DAKBVG010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.240"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            218..1066
                     /locus_tag="OUD09_002496"
     CDS             218..1066
                     /locus_tag="OUD09_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HCT9693725.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            1080..1403
                     /locus_tag="OUD09_002497"
     CDS             1080..1403
                     /locus_tag="OUD09_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693726.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KNKRF"
     gene            1400..2151
                     /locus_tag="OUD09_002498"
                     /pseudo
     CDS             1400..2151
                     /locus_tag="OUD09_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291626.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
     gene            2141..>2770
                     /locus_tag="OUD09_002499"
     CDS             2141..>2770
                     /locus_tag="OUD09_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693727.1"
                     /translation="MTIKREKWLRGVYSFVILSVGAPIFVVANQLQNSYLLILTIGVL
                     CLIVCCIPKKWVRLLLSLPVMIGCLFLYFPSKTFSILWILQFFSSVTEEFFHLSQGKL
                     YYLPEKTAFFLIMLLIYLFITLTVDYDYWYAPFLSLMAYFMMLTVFRKKDLLYEMIAV
                     VTVLFVYLAARQFFSIRLLSGNTRFQSLTTVLLICFLTFSAILPLYLPKV"
BASE COUNT          814 a          527 c          521 g          908 t
ORIGIN      
        1 gtattctcat ttcaaaacga atgtacttta aaaaaacata ataagatcac tttgcttgta
       61 ggatgtattc ccttccatta caatgaaata aatagcgatt ttatgagaag gtgatgtgaa
      121 aatggaagaa aaaatagaag aatccaaaca aaaaatcgat cagttgatat aagaattaga
      181 aaaagcggtc atcggcaaac gttcaactat ccaattgatg atcgtggccc tattagcagg
      241 tggtcatgtc ttattcgaag atattcccgg tgtaggtaaa acgttactgg tcaaagcact
      301 agcaaaagct gtacagggaa ccttcagcag agtccaatgt actcctgatc tgctgcctag
      361 tgatattctt ggtttttccg tatacaattc acaatcaaaa gagtttgaat ttcgtccggg
      421 accgatcttt accacgatct tgttggcaga tgagatcaat cgtacgactc ctcgtactca
      481 gtcagcactt ttagaagcaa tggctgagaa tcatgcaacg atcgacaaca atacataccc
      541 tctagacaat catttttttg tcttagctac gcaaaatcca atcgagtacg aaggaacata
      601 tcctttgccg gaagcacagc tcgatcgttt tttattccga ctacaaatcg gttatccttc
      661 ttttgatgac gaacttttgc tacttttgga taaattcgaa aaagtgttgg ataacttaaa
      721 tgaagtattg aacagttatg aaatagaaga aataaaaaca aatgtggcga atgtctttgt
      781 cagtcccgaa gtggcatctt atgccttgca gttagtgtca gcaacaagaa gtcatgctgc
      841 catccagctt ggcatcagtc cacgaggaag cctttccttt attcaggcag caaaagctta
      901 tgctttaatc catgggagaa attacgtcac gcccaaagat ttgcaggatt tagttcctta
      961 cgtgttttcc catcgcctga ttttttacga tcgttcattg aaggaagacg aaaagaaaca
     1021 aatcattcat actcttgttg cacaaattcc tattcctgtg aggtagattg aggtagacaa
     1081 tggaaaaaat caaagattat ttcattaatt ttgtgttatt tctggtatat ttcatcattg
     1141 tgctttttgc tcttgtattc aacaattctt taggctggtt cttgttggcc ttttttactc
     1201 tttatgtatc atggagcttg atttcattaa gcagctttct aaagaaactt tcggttactt
     1261 gtacattgca gccactcgtt tatcgcaaaa aaagtatgca ggttattttt tccatagcaa
     1321 aaaagagaac tttttcttta cctctccctc gtttacaaat cggcttccca gcaatctttg
     1381 cagaaaaaaa caagagattt tgattcttac aaataaaccg caagaagtcc aattgctttg
     1441 gcagccaaaa gaaagaggat gccatgaaac attaactgtc atttttacag cctatgatag
     1501 tctaggactt ttcagaaaaa gtgcaaaaag agagatctct tttcctgtga ctgttctgcc
     1561 tgctttttgt aagattcaag cagaaaaatt acagagagtt ctggaaaaaa acagcagctg
     1621 aacgcgtacc aaagaggtct tgattttcgg gaacatcgcg cttatcgtcc aggtgattct
     1681 ttcaaccgaa tcgattggaa attgtctgct catactcaag agtggttata ccgtgaatac
     1741 gattatcaag aagaagaatg ttctcctctt ttttgctttt ggggttcgct cagtccaaaa
     1801 tttgaacctc tgctagactt gtatttttct ttatggcatt tgagaaaaga aaagcagccg
     1861 gaacttctga tattaggcaa ccgcgccttt catggcaaag accccggtca tacctatttt
     1921 gcttctttgg aagctggcga tgagaaagct tttttcgcac acttgaaaaa atatgcaaaa
     1981 aaacaaatcg tcctctttgt tcctgagaat tctcctgaag tctcagaagc tattgaaaca
     2041 ttgtcaaaag atcgaacggt ttatcttgtt tatttcgtag aaaaacaact catggtccaa
     2101 gaaaaagaca agatatgttc attgccagga ggtgaatgga ttgacgatta aacgggaaaa
     2161 atggctgcgt ggcgtttact cctttgtgat actaagtgta ggtgctccga tatttgtagt
     2221 agctaatcaa ttgcaaaaca gctaccttct tatcctaact attggtgtcc tatgccttat
     2281 tgtctgctgt attcctaaaa aatgggtacg tttgctgctt tctttgccag taatgatagg
     2341 ttgtttgttt ttatattttc cttcgaagac attttccata ttatggatct tacagttttt
     2401 ttcgtcagtc acagaggaat tcttccatct atcccaaggt aaactttatt atttgccaga
     2461 aaagacagct ttttttctca taatgttgct gatctattta tttattacat tgaccgtgga
     2521 ttacgactat tggtacgcac cctttctttc tttgatggct tacttcatga tgcttaccgt
     2581 ttttcggaaa aaagatttac tgtacgaaat gattgcggta gtaactgttt tgttcgtgta
     2641 tctggcagca aggcaatttt tctccatccg gttgttgtcg ggaaacactc gattccagtc
     2701 attgactaca gttctgctta tttgtttctt aacttttagc gctatcttgc ctttatatct
     2761 tccgaaagta
//
LOCUS       DAKBVG010000239         2770 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000239 DAKBVG010000000
VERSION     DAKBVG010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.241"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(<1..339)
                     /locus_tag="OUD09_002500"
     CDS             complement(<1..339)
                     /locus_tag="OUD09_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCT9693728.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
     gene            complement(356..760)
                     /gene="tnpA"
                     /locus_tag="OUD09_002501"
     CDS             complement(356..760)
                     /gene="tnpA"
                     /locus_tag="OUD09_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HCT9693729.1"
                     /translation="MKLDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
     gene            complement(923..1669)
                     /locus_tag="OUD09_002502"
                     /pseudo
     CDS             complement(923..1669)
                     /locus_tag="OUD09_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
     gene            complement(1673..2731)
                     /locus_tag="OUD09_002503"
     CDS             complement(1673..2731)
                     /locus_tag="OUD09_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HCT9693730.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
BASE COUNT          856 a          567 c          468 g          879 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgccaccag
      421 ttgtcagaag acaaaaactt ttagaccaga acatttcttt ccaaagaaat tgtttgactc
      481 ttggaaagtc acgttttatc aatcttgagc tggcactttt ataggcattg atgaattttg
      541 tcaattctgt tttaggttga gctttgaaca taatatgaac gtggtcttta tcatgattcc
      601 actccactaa agtaatgtga tacgattctg aaattctttc aaaagtagtt ttggcataat
      661 cagatatttc atcatcaatc acttgtctac gatatttcgt tactaaaaca aggtggtaat
      721 aaagaagaaa tactgaatga ttatttgtat ctaatttcat gttatatcaa ctcatttctt
      781 gtatagactg attgtaacat gggacaaaat aaaaaggcaa tacgccttag ttgtcggtct
      841 tcgagttacc aacggtaacc ctcataccga acggcattca tcacccacct atagaggatg
      901 ggcgacttct gcctaattct gttaaaatag cttcattatc ttgaagctct ccacgggctg
      961 tattgatcaa gatgctgcct tttttcattt gagctaaaaa tccttcattg atcatttgat
     1021 cgttttcacc tgggatatga ggtacatgaa tactgacgat atcgcttttg gctaataact
     1081 cttcaagcga gtggaacgtc aatacttttt tagcagcttc tgtctgatga atatcgtagc
     1141 cgataacttt ggctcctagt cccttaaaca aaacagcttc cgtcaaccct atcctgcctg
     1201 ttcctatgat ccctactgtg caatttctga tttcacgact aaacatttgc tcatccactc
     1261 gaaaatcttt gaaagatgtt cggatcgtcg tatacgcagt atttctcaaa agcatcatgg
     1321 caaaagtcaa agataactct gctatcgcat ttggcgaata cgatggtaca cgggctactg
     1381 tcataccaaa atcagctgcc gcttgtagat cgatatgatt gactcccaca gtccgtgtaa
     1441 aaacataaca aatcccatat tcctgcattt ttgcaagatt ggtctcatct gctatacaat
     1501 ttcccctcaa caatacagca tcatggtcct ttgctgacac gatattgtca tgtgtcaaca
     1561 gttcttcata aagactgaga tcataatggt aaatattcaa tttttcaaaa aaatctatct
     1621 cgttcggacg aacaccgtaa catgcgattc tcatactttc ttcctccatt tcttatgaaa
     1681 ataaacctat gccatgaatc atttgtaata caacgatcca tacaggtgtc agtaccacag
     1741 caaaaatagt tgacagtaag gatgcattgg aggctaaaag tgcctcccta tcaaaactga
     1801 tagcataggc tgctgccact gttgcagctg gtgtagccat catgataacg attgttgcca
     1861 gtgcttcaaa attgactggt agaatattcg ttgccgtcaa aactgctaga atcacgatat
     1921 tcaatgcagg tacaaatacc actttgacta gactgtaata ccatgaagtt ttttcagatg
     1981 cagctttttt gaagcttact tcacctaatg ttgcgccgat tgctagccac gccaatggtg
     2041 aacttaaacc agctaaataa tccatcggct gaaatagcca cggtgcagtc tggtcaattc
     2101 gcaaaaacgc tacttggctt atctctcctt ctgcacctgt aactgacact tgtggcagat
     2161 acccttggaa cagccaaatg aaaaggccgg caaaagtagc gataatgatt ggattgacga
     2221 acatcgcttt gatattttta gcttccatct tcaagccact catttttatg tatccataag
     2281 aatacaaaaa gattcggtag ccaatattga aaattgagga atacatcacg ccgacaggac
     2341 cataaatcgc actgacgata ggcgttccga aaaaggtcgt agaaccaaag atcgtcagga
     2401 cacgcaacgt tgtttgctta tcccctttat agcttaagaa aaaaggtttt gaaactagaa
     2461 taagaataat ataaataact aatccccaaa tcaagacatt gattccttgc atcagtaatt
     2521 ctgtattgat atcttgcatg aaagctcgaa atgccagtgc tggcaaagcc actgttaaga
     2581 cgactttcga tagaattttt ccaacagatt cagaaaaaat cccctttttt ctacaaacaa
     2641 accctaataa tataataaaa actgttgaag tgattgcact gacaatcgac atatctgtca
     2701 aagtcgatcg aaaaatgtca aataagttca tctttctcct cctaattatg ttcatttatt
     2761 cacaataaaa
//
LOCUS       DAKBVG010000240         2740 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000240 DAKBVG010000000
VERSION     DAKBVG010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.242"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(<1..625)
                     /locus_tag="OUD09_002504"
     CDS             complement(<1..625)
                     /locus_tag="OUD09_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCT9693731.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLD"
     gene            773..2062
                     /locus_tag="OUD09_002505"
     CDS             773..2062
                     /locus_tag="OUD09_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCT9693732.1"
                     /translation="MRYSTILISTFAALSLSLTINQTVHTEEIETAKWTETKWANVNR
                     ITFQDEFSSFNSGVAKLRKGQTATIKAKIDYSGDLAPILQSAVTFENVDPALSIGYDE
                     NTLTIQHKQAIFTFTVTLNQDLLKPALFTVKVSDGLPNDHHHLTPYSQRQTIEQDSAI
                     DSGGDLVEEPTDKPENENKPEVPPTENPDGEQKPEIEPGEEPDTETQPEPDNESKPEI
                     TPGEKPDVDPEEKPDVTPEPDTDSGNQTVPETNPDTDNETENPEKPEVAPEEKPDVTP
                     EPDTDSGNQTVPETNPDTDNETENPEKLEVDPEEKPDVTPEPDTDARDQGIPEKINKK
                     TIQEDGKKESKKSNLAILKINEEQLNKKSRIFDSAQSAETLKSSKDTTFASPETKNKQ
                     LPKSGESQNKVILWSGIILLSIATMLSAKRFKQNRSL"
     gene            complement(2188..>2740)
                     /locus_tag="OUD09_002506"
     CDS             complement(2188..>2740)
                     /locus_tag="OUD09_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCT9693733.1"
                     /translation="EKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQFV
                     IALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGIRD
                     VVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIPIF
                     TFFLDVLKVKKIPNHLPKETDIP"
BASE COUNT         1078 a          503 c          466 g          693 t
ORIGIN      
        1 ggtcaagtac ttctttttta aaaaaagtat tcatcataaa agccactact tgcggatctt
       61 catcataaaa ttctgtagca gtacttgaaa agatgttcca ataaatatct aatgctgcat
      121 attgaatttg tacctctttt ccagaaaaag actttctgcc attattagaa aaggagatag
      181 acaaaccgta caatgctaaa aatgcattaa tctttttcat tttcgtatac aaataagagg
      241 cactatagtt tgtttgctga cttaaactga taatcgaatt tgtctgacag ctgaacaaag
      301 aagtcaagat ctgataactg actgattcgt tgcagtatct tccaaataga cgataatagc
      361 agtaagccgg atcaattcct tcaaaattca actttaaaaa aggtttttgc ttatcaatat
      421 gcagttctgt tagattgaga cgctcaaagt cttgttctaa ttctttgatg tatagtaaaa
      481 aatttgattc agacatatgg aaatgatctg ctaaatattg tgtagtaata aagaaacgat
      541 gttcagatag gaaaagaaaa atatctaatt ttgtattgct ttttttcttc aagagaaacg
      601 ttttaatcgc ttcttgagtt tgcatgaaag aggctccttt ttacttaaat tgttaaaact
      661 tgaaaatatt actgtttttt tcataaattt agacaaaaaa acagtaaaca tataggtaga
      721 atctctacta tcttatttct tgcatacaaa aaaatcaagg agtgagagag ttatgagata
      781 ttcaaccatc ttaatatcaa catttgcagc cttaagctta tcgcttacta taaatcagac
      841 agtccacaca gaggagatag aaacagccaa atggacagaa accaagtggg ccaacgtcaa
      901 tcgaattacc tttcaggatg aattttcatc cttcaattca ggagtcgcaa aattaagaaa
      961 aggacaaaca gcgaccatca aagcaaaaat tgactattca ggagacttag cacctatact
     1021 acaatcagct gttacatttg agaatgttga tcccgctttg tcgattggct atgacgagaa
     1081 cactttgact atccaacaca agcaagcgat atttaccttc accgttacat taaatcagga
     1141 cttgttaaaa ccagcgcttt ttactgtcaa ggtatcagat ggactaccga acgatcatca
     1201 tcatttaacg ccgtatagtc agcggcagac aattgagcaa gattcagcta tcgactcagg
     1261 aggagatctt gttgaagaac caacagataa accagaaaat gaaaataagc cagaagtacc
     1321 gcctacagaa aatccagacg gagaacagaa accggaaata gaacctggag aagaacccga
     1381 tacagaaact caaccagagc cagacaatga atcaaaacct gaaataaccc ctggagaaaa
     1441 accagatgta gacccagaag aaaaaccgga tgtgactcca gaaccagata cggattctgg
     1501 aaaccaaaca gtaccggaaa caaatccaga cacagataat gaaactgaaa atccagaaaa
     1561 accagaggta gccccagaag aaaaaccaga tgtaactcca gaaccagata cggattctgg
     1621 aaaccaaaca gtaccggaaa caaatccaga cacagataat gaaactgaaa atccagaaaa
     1681 actagaggta gatccagaag aaaaaccaga tgtgactcca gaaccagata cggacgcaag
     1741 agaccaagga ataccagaaa aaataaacaa aaaaacaatt caagaggatg gaaaaaaaga
     1801 gagtaaaaaa tcaaatctag caattctaaa aataaatgaa gagcagctga ataaaaaatc
     1861 acgaatattt gatagcgccc aatcagctga aaccttaaaa agtagcaagg atacaacctt
     1921 tgctagtcct gaaacgaaaa ataaacagct accaaaatcc ggagagtcac aaaacaaagt
     1981 tatactgtgg agcggaatca ttctattaag tatagcaaca atgctttctg caaaaagatt
     2041 taaacaaaac agatctctct aacatttgaa taatgtctgt gaacgcattt ttcaaataaa
     2101 aagaaatatc cagaaaacga ccccaaaata ccagattgaa atctgatatt tggggtcgtt
     2161 catactttat ctttcttcat atttatttta agggatatct gtttcttttg gaagatggtt
     2221 cgggatcttt ttcactttca atacatctaa aaagaaggta aagatcggaa tgccgacaat
     2281 caagccccaa acgccaaaga accgttcgct aacaagtaag accacaaatg tataaaaaat
     2341 cggcagttct gttttgctgg acatcaactt cggattcagc acataagact caattagatg
     2401 gacaatcaaa atcgtaatca ggatataaac aacatctcgg atgcctcctt gggaataagc
     2461 tatgaaagat agaggaatac aagaaatgat aacacctgct actgggatca gacttaaaat
     2521 gaaaatcatg atcgctaaac tcagcagctg atgaaagcca aatacagcca aggcgattgt
     2581 agtgagcaac gtgtttaaaa gagcgataac aaattgagct tctaatacca aaccaaatgt
     2641 attgacaaac ttatcggcaa aatagtaaat atcttgaaag aaccaagaaa attcactttt
     2701 taaaaacaat cttgagaata caattgtttt tttcttttca
//
LOCUS       DAKBVG010000241         2710 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000241 DAKBVG010000000
VERSION     DAKBVG010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.243"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(562..1065)
                     /locus_tag="OUD09_002507"
     CDS             complement(562..1065)
                     /locus_tag="OUD09_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HCT9693734.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1424..2449
                     /locus_tag="OUD09_002508"
     CDS             1424..2449
                     /locus_tag="OUD09_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCT9693735.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
BASE COUNT          878 a          420 c          493 g          919 t
ORIGIN      
        1 caaacagtat aaaacggtgt ggtttttgga ataaaaaaat aacatatctc attttacgct
       61 tttgttattt tatacttaaa aaataacgac acagaaaata ccgtgtctca gatttctcta
      121 ctacttctaa aaactactgt ttttttcctt tgaaatgcct tcaaaaagta ggatcaggca
      181 gaatatactg gcagaatcaa ggagaaggga gcctaaataa ggagaaaaac catagaaaat
      241 ataaagtaat tacttataaa acaagctttt tatagaaaaa taatatacct gagtagggta
      301 taaattaaga cggaattcct ttctagattg agatattgac taggaagaat gttaagattt
      361 taagccttta agctaaaaaa aaaccctttt tgttaaataa ggggaatata gcctgtcagt
      421 ttcaaactgc aagtcgtcct gctgcaataa tatagtagat tagagatgaa aatagatttt
      481 tttaaaccaa tgactgaata aaaagaccgt catagataca cattggaatg tgtctattac
      541 ggtctttttt tacgaacgat tttattgaag acggacagtt cgggtcaaaa ataaaaaagc
      601 tacgatcaga aataagaata aaatgtatcc caaaataatg gctagacggt ctaacttatt
      661 tgtcgataga tgtttgttta gaagttgata ggcccataaa cctatcagtc ctcctgttgt
      721 attagttatc aaatcagtga tgtccgctac gccaataccg aataggaatt gaatcaattc
      781 tgccaagaag cttacaagga aaataatccc taatttttta gaaaaagaaa gtctcttgaa
      841 attgacattc aaaagcaccc caaaaggaaa aaagacaatc aagttataag tggtctcacg
      901 taataccgtc gtattttcaa aagaaaaagt agaaaaaggg acgagattga cattgctgta
      961 agaataagtg agaaattgtg gaagattaaa tgatagcttg aataaaatca accataatag
     1021 aaagaataaa taaatcccta ataaaaattt tgatacatac cgcatagaca tctcctccat
     1081 ttaaaaagta taccatcgaa aaccgctgaa aaatcataaa aaagtatgaa gtttaaggac
     1141 caacgtagtt atcgtacgaa aattagaagc ttttttttgt tgggaatccg gtttctttaa
     1201 caaagctaag atagcgagtt gcttggaaaa gttgctatct tttttctgtc aaaacgactc
     1261 ggaatgttcg ttttttgttc taatttctcc aagttgttta ctttttgata ctaagtttat
     1321 gaaaaatcaa gaaatagttg ttatactagg taatgctgac aaacgggatt ggcatttcat
     1381 cttggttcag ctttttttcc aattactagg aaggagggat agattgttca agacttattt
     1441 tcgtttcaat aaaatacaag ataatccttt tcgtatcatg aatctgatgc tacttatctt
     1501 tattgtatta tggttaggtt acataaatca tgattttttg atttcaagtt tttcatcatt
     1561 agggatcttt acttttttct attatcaaaa cataccgatg aaatatttgc tgaaacgtat
     1621 gtttctgatc ggttgcggac tgttggttgc tttcgttttg gggatcttat cgacttatgt
     1681 tttatggctt gaaccatttg ccgtagcttt ggttgctttt tccagcagat tcgtgttgcg
     1741 attattccat atttctaaac caggtggctt gttttttgcg atgctttctg ccatgggaac
     1801 gagcatgcag ctgccaatcg ctcaattacc gatcgtctca ctttattttt ttatgggtgt
     1861 cgtttttgcc ctgattgcgg cagtgattac aaaattactg gattcacggc ctgagcagac
     1921 aattgaaaaa gcaactttaa aagaaagatt tcacgaagag cctttagtga tcattgattc
     1981 tgttttctac agcgctgcct tatttctttc cgtttatgtc agtcacggct tgaatctgca
     2041 taatccttat tggttgacct tgtcatgtgc atcgattttg ctagcagaaa acttggatgc
     2101 gatgaagcat cgacaagtcc aatatctgat tggatcgatg ggtgggttat gtgtgtcggc
     2161 attcttgtca tttgtgccgt ttacgcagtt gcagaccatt tttctgatca cttttctata
     2221 tggaatcgca caatttctag ttgctagaaa ttatgcagtt gctaatattt ttcttaatcc
     2281 gatggcgttg atgctatcta cacttattcg tggagcatac ttgatttctt tgatcgaata
     2341 tcgtttttta gggatcgtca taggaagttt catcggttta ggtgttgcgt gggtaatgac
     2401 ggttggtttg caacattatt taacggttgt tcgtcaaaaa ttagaataga aggacaaata
     2461 tatgtgccaa aagaggttat gacaagattt ttgtcacaac ctctaattca aggtgttcaa
     2521 aagccagatt ctcagcatta agtcaaattc acacaaaaat gtggaaagga actttcaaaa
     2581 aaattttata tttattctat caaaaaattt tatatttatt ctatcaaaaa attttgttta
     2641 tgatggtaac tgaataaact ttcctgagat ggtcaatgct ttccttgaaa gtcttacaga
     2701 tattctttct
//
LOCUS       DAKBVG010000242         2689 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000242 DAKBVG010000000
VERSION     DAKBVG010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.244"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            <1..932
                     /locus_tag="OUD09_002509"
     CDS             <1..932
                     /locus_tag="OUD09_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HCT9693736.1"
                     /translation="DYIKRIDSDSFLAAFYLYTAKYWIDDSRKTIKYAKRKKQFYFQT
                     WHGTPLKKIEFDAKDKLPKRYLSYAKKDSESITYLLSGNRYSSEKYVSAFNIVPSKIL
                     EVGTPRNDELASSKEQVFDLKKKQINVLFAPTFRNNIEDNGITQLEWLGVDNLREFFK
                     KQQQELNLMTKFHPNVHSKLATDSKSIEYMKKHQITLINDGIPLEMLFEEIDVLITDY
                     SSIFFDFALTKKPIILFNYDEKEYSLERGFYIQLSELPIISVQDSYGLVDVFKNQQEK
                     LLKSSQELLDYIGNYETGASTEKVLELLERDIK"
     gene            929..2356
                     /locus_tag="OUD09_002510"
     CDS             929..2356
                     /locus_tag="OUD09_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HCT9693737.1"
                     /translation="MKKTVKDIFYNAIYQVFLIVLPLLTIPILSRRIGSTGLGIYGYV
                     FSISQFLMTVIAVGMNPFRIRNIAKSRKDKKALSLQFWNIYFLQFLIGLSVSFLYIAY
                     IYFFDVKYKNLYLIQLIFIAGLTLDISWFFQGIEEFSKVVIRNTVIKLSSVLLIILFV
                     STKDDLWIYVFITSITNFLGSLVFWLSIHGKVGKPLFNSNVFKQLWKPAFIILTPQLF
                     MQVYTTLDKTIVGYFVDPTALSYYDQSQKIARIILAVLTSITIVMLPKISKAQAEGKS
                     ENILVYTKKSFDYTIIIALILFSVVFINTKEFVPWFFGENFKPMTINMLISCLIIVVS
                     PIGGVFANQFAIAIEKDKEYAYPMIIGAIVSIIGNVAFVPTYGAVAATIVLVVVELLV
                     FFFRVFFVRKEIKISYFFGKNVVLFFIVNLTLTTSAYYFLPQLFSSSFLDMAIKSIMI
                     FIIYLPFVYFLFPDIRNEVRKIIRK"
     gene            complement(2423..2587)
                     /locus_tag="OUD09_002511"
                     /pseudo
     CDS             complement(2423..2587)
                     /locus_tag="OUD09_002511"
                     /inference="COORDINATES: protein motif:HMM:NF013753.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          904 a          350 c          441 g          994 t
ORIGIN      
        1 cagattatat taaaagaatt gattccgatt cttttttagc tgccttttat ttgtatacag
       61 ctaagtattg gatagacgat agtcggaaaa cgattaagta tgcaaaaagg aaaaaacaat
      121 tttattttca aacatggcac ggtacgcctt taaaaaaaat tgaatttgat gcaaaagata
      181 agttacctaa acgttaccta agctatgcga aaaaagatag tgagtcaatt acatatttat
      241 tatctggtaa tcgctatagt tctgagaagt atgtttcagc attcaatatc gttccttcta
      301 aaatattaga agtaggaact cctagaaacg atgaattagc ctctagtaaa gagcaagtgt
      361 ttgacttgaa aaagaaacaa atcaatgtct tatttgctcc aacttttcga aataatattg
      421 aggacaacgg aattacgcaa ctagaatggt taggtgtgga taacttacgt gaatttttta
      481 aaaagcaaca acaagaatta aatttaatga ctaaatttca tcctaatgtt catagtaaat
      541 tagcaactga ttctaaaagt atagaatata tgaaaaaaca tcaaattact ttaattaatg
      601 atgggattcc tcttgaaatg ctttttgaag agatagatgt tttaatcaca gattattcat
      661 ctattttttt cgattttgcg ctaacaaaaa aaccaattat tctttttaac tatgatgaaa
      721 aagaatatag cttagaaaga ggtttttata tacagttaag tgagttacca attataagcg
      781 tacaagatag ttatggcctt gttgatgttt ttaaaaacca acaagaaaag cttttgaaaa
      841 gttctcaaga gttacttgat tatattggaa attatgaaac aggggcaagt acagaaaagg
      901 ttcttgagtt gttagagagg gatataaagt gaaaaaaaca gtaaaagata tattttataa
      961 tgcgatatat caagtctttt tgattgtttt gcccttactt actataccta tacttagtcg
     1021 tagaataggt tcaactggtt tagggattta tgggtatgtt ttctcaatat cacaattttt
     1081 aatgactgtc attgctgtgg gaatgaatcc ttttcgtatt cgaaatatcg ctaaatcaag
     1141 gaaagataaa aaagcactat ctttacaatt ctggaatatt tactttcttc aatttttaat
     1201 aggcttgagt gtttcctttc tctatatagc ctatatttat ttttttgatg taaagtataa
     1261 aaatttatac ttaattcaat tgatatttat tgcagggcta acattagata tttcttggtt
     1321 ttttcaagga attgaagaat tttcgaaagt ggttattaga aatacagtta tcaaattatc
     1381 atcggtattg ttaataatcc tttttgtttc aactaaagat gatttgtgga tttatgtgtt
     1441 cattacttca attactaact ttttaggaag tttagttttc tggttgagta tccacggaaa
     1501 agtaggtaaa ccactcttta actctaatgt ttttaaacag ttgtggaaac cagcttttat
     1561 tattttgact cctcagttgt ttatgcaagt gtacacaact ttagacaaaa cgatagttgg
     1621 gtattttgtg gatccaacag cattatctta ttatgatcag tctcaaaaaa ttgctcgtat
     1681 tatattggca gtcttaacat caattactat tgttatgttg cctaaaatat ctaaagcaca
     1741 ggcagaggga aaatcagaaa atattttagt ttacacaaaa aaatcttttg attacacgat
     1801 tattattgca ttaattcttt ttagtgtggt gtttatcaat acaaaagaat ttgttccatg
     1861 gttttttggg gagaatttca aaccgatgac aataaacatg cttatttctt gtctaattat
     1921 agttgttagt ccaattggtg gagtttttgc taaccaattt gcaattgcta tagaaaaaga
     1981 taaggagtat gcttatccaa tgatcattgg tgcgattgta agtattattg gaaatgtagc
     2041 ttttgtgcct acttatggcg ctgtagcggc tacaatcgta ctcgtggtag tagaattatt
     2101 agtctttttc tttagagtat tttttgtacg aaaagaaata aaaatatcct atttctttgg
     2161 gaagaacgtt gtcttatttt ttatagtcaa tcttactttg actacttcgg cctattactt
     2221 tcttccacaa ttattctctt cttcattttt agatatggca attaaatcaa taatgatttt
     2281 cattatttat cttccgtttg tttattttct ttttccagat atacgaaatg aagtaagaaa
     2341 gattattcga aaataaaata atgttgtaaa aaagtagttc tttgtcaaat cgggctgata
     2401 aggtcattta tgatcaacaa agttagtaag atttactagc ttttaatgca tagcttatta
     2461 acccgttgat tagaaaaatc cacaagcgca tattggtaaa actgcaaaat ccataagcaa
     2521 atcgtttcaa tgttttgata tgtgtgcgaa gattctctaa ttttgtatta aaatactttt
     2581 tcgaaaatgc tagttaaaag cctttttcag atatgaaaaa ggctttttaa gatagtgaag
     2641 tttcgaggga acttctttgg tcatttctcg aagtagctag ctgtttctt
//
LOCUS       DAKBVG010000243         2688 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000243 DAKBVG010000000
VERSION     DAKBVG010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.245"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            <1..513
                     /locus_tag="OUD09_002512"
     CDS             <1..513
                     /locus_tag="OUD09_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HCT9693738.1"
                     /translation="IPYIAAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEI
                     NQYREREPEIQPAEVKQEHKNTVETAENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLK
                     DAAFIEDPQHNQVILKGKSYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYY
                     EQQLSQKERV"
     gene            574..2043
                     /locus_tag="OUD09_002513"
     CDS             574..2043
                     /locus_tag="OUD09_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCT9693739.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVGDDRLDHAVAFLWKDPQTGETVGASYQGTIVDFNRFGKRGTYKHIDKNPTPNHGFN
                     LKIGDPKHLKFFESSIDLLSYAALNREKLQDAWLVSMDGLKHHVISHYVEESISELRR
                     KQTFPQSIEICVDNDRAGHIFYEKEQMKGIVDPFTNKKIRCERGIPNDWQVPKEYKAT
                     YEAVAKEMSVEPEAIMAIHKTETNLQLTNQLVSAHDVQSTFGKMLAKGEPVETIDLKE
                     ACTTVAKELKVCERADGTYNFDRFYSRKANIKDVNAGILLSYKAEQYYKGYKKHEHEF
                     VPEVKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQERE"
     gene            2054..>2688
                     /locus_tag="OUD09_002514"
     CDS             2054..>2688
                     /locus_tag="OUD09_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCT9693740.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELYSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEVQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQ"
BASE COUNT          993 a          444 c          581 g          670 t
ORIGIN      
        1 attccttata ttgcagcatg gacgaagaat ggtcagacgt tggatgataa ggaagcggcg
       61 gaacgtggga aaatcatgaa tgatgtcagc cgtgtcgcaa atgaattcat tcaaaccatt
      121 tctaatgaaa tcaatcaata cagagaaaga gaaccagaga tccaaccagc agaagtgaag
      181 caggagcata aaaatactgt agaaacggca gagaataaga atattgtgcc cgttggttca
      241 ctttggatcg acaagaaaga cgggaaagta atggaagaag atacgggtcg aacgttacat
      301 ttgaaggatg cggcgtttat tgaagatccg cagcataatc aagtgatttt aaaaggaaaa
      361 agttatggaa aagagattca aacggcgtta ccttcagaag aatttgcgct aggaaatcca
      421 ttccgatttt cacttgaaac agtgtcagaa gtggccaaac gaaaaaatga aatctattat
      481 gaacaacaat tgtcacaaaa agaacgcgta taaaacaagt aaaaaagact atctaaaaaa
      541 cggatagtct tttttctttt gggaggtttg aaaatgaccg aaaaaaaaat gagtcttatt
      601 gatcgatgta aacagatcga tatagtggat tttgctcgaa acaatggaat ggctgtagtc
      661 aataaaggac gagactatcg cttagaagac catgattcat ttgtttttga tcgaagaaaa
      721 caacgttttt attggaacag tcaaaatatt agtggcgaca ttattgaatt agctaaactt
      781 tttttcatag acaaagagat ccaagaccct aagcaacaat ttaaagcagc cactgatttt
      841 attctaaaga atgaagacaa aacagaacga gtggaaaatc ttcattttga gacagaaaag
      901 tacaaagatc accccgttga ttatcaacca ctcacagaaa aaggaaggaa ttatctgaag
      961 gaagaacgaa aactaccaga gtggctgatt gattatgcag aaaaagaagg gttgattgct
     1021 gaactaaagc cgaaacatga acggcaaaat tttttagttg gagacgaccg tttagatcat
     1081 gcggttgctt ttttatggaa agatccacag acaggagaga cagtaggggc aagttatcaa
     1141 ggaacgatcg tcgattttaa tcgtttcggt aaacggggaa cgtataaaca cattgacaag
     1201 aatcctacgc caaatcacgg atttaattta aaaataggcg atccgaagca tttaaaattc
     1261 tttgaaagta gtattgatct gttaagttac gctgcattga atcgggagaa actacaagat
     1321 gcctggttag tttcgatgga cgggttaaaa catcatgtaa tcagtcatta cgttgaagag
     1381 tcgatttctg aattaagacg aaaacaaaca tttcctcaat cgattgaaat atgtgtggac
     1441 aatgatcgtg caggacatat tttttatgaa aaagaacaga tgaagggaat cgttgatcct
     1501 tttaccaata agaaaatccg ttgtgagcga ggaattccaa atgattggca agtacctaaa
     1561 gaatacaaag ctacttatga agcagtcgca aaagaaatga gtgttgaacc tgaagcaatt
     1621 atggccatac acaaaactga aactaattta cagctgacaa atcaattggt gtctgctcat
     1681 gatgttcaat ctacgtttgg aaagatgttg gcgaagggcg aaccagtaga aacaattgat
     1741 ttgaaagaag catgtaccac ggtcgcgaag gaactgaaag tatgtgaacg cgcagatgga
     1801 acatacaact ttgatcgttt ttatagtaga aaagccaaca taaaagacgt taatgcagga
     1861 atccttcttt cctataaagc agaacaatac tacaaaggct ataaaaagca tgaacatgaa
     1921 tttgtacctg aagtgaaaaa ggattggaat gatcaattga agcatgaaat ctaccaacaa
     1981 gaaatcagaa agcaaaaacg tgcgatgttg tttcaacaag gcagacaaca agaaagggag
     2041 tgaagtcgtc actatgaaaa ccgtcatttt agcagaaaaa ccgtcacaag cgaaggctta
     2101 tgccgatagc ttttctaaag ctacccgaaa agatgggtat tttgagatcc aagatcgatt
     2161 gtttacagga gaaactgtga tcacgtatgg ttttggtcat ttagtcgagt tatattcgcc
     2221 cgatatgtac gatgaaaaat ggaaacaatg ggcattagaa catttgccta ttttccccaa
     2281 tcaataccac tatcatgttc ctaaagataa gaaaaagcag tttagtgtcg tcaaacgaca
     2341 gctgcagtca gctgatacga ttgtgatcgc gactgatagc gatcgtgaag gggagctgat
     2401 tgcctggtca atcattcaac aagcaggcgc caatcaagga aaaaccttta aaagactttg
     2461 gatcaactcc ttagaaaaag aagccattta tcagggattc caacagttac gagacgcaga
     2521 agagacctat ccaaaatttg aagaagtaca agcacgtcag atcgccgatt ggttaattgg
     2581 gatgaacggt agcccactgt atagtttact cttgcagcaa aaaggcatac caggaagttt
     2641 ttctttaggt agagtccaaa ccccaacact ctatatgatt tatcaatt
//
LOCUS       DAKBVG010000244         2674 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000244 DAKBVG010000000
VERSION     DAKBVG010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.246"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            316..1101
                     /locus_tag="OUD09_002515"
     CDS             316..1101
                     /locus_tag="OUD09_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCT9693741.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            1114..1965
                     /locus_tag="OUD09_002516"
     CDS             1114..1965
                     /locus_tag="OUD09_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HCT9693742.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(2089..2541)
                     /locus_tag="OUD09_002517"
     CDS             complement(2089..2541)
                     /locus_tag="OUD09_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCT9693743.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLMQTDISLVS"
BASE COUNT          846 a          448 c          553 g          827 t
ORIGIN      
        1 gctgtgacaa aagtcttgtc acagcctttt tttccaaata aatggttttt cagaagtagc
       61 ttcttcggaa ataagccgaa atttcacaaa aatttgagaa gtaatttttg taaattactt
      121 cttatttctt gaagctggcc acttctgtca caacctcttt ctttatagaa ataaaaggac
      181 actagcaatt catgaaaacg attgctagag cgtatcattt gctttagact atatatcttg
      241 gtgtacactc actttaagac ggaaataaaa gttatccgta ataacaggtt taggtatgaa
      301 aaaggagtgg gtactatgta taaggaatta gaaaacaaag tcgcagtagt aactggtggc
      361 tcaaagggga ttggaacagc gattgctcaa cgattcggga aagaaaaaat gaatgtagtt
      421 gtcaactata actcggataa agaaggagca gaaaaagcag tcaaagcaat cgaagaggca
      481 ggcggaaaag cagtctctgt acaagcagat gtcagttctg aagaaggaat acagcgttta
      541 cttgatacgg ctttagaaaa ttttgggaca ctggacgttt ggattaataa tgctgggatg
      601 gaaaatcagc atccaaccca tgaacttagc cttgaagatt gggaaagagt attaaaagtc
      661 aatttgactg gcgtttttat cggaacgaaa gcggctttaa attactttac agagaatgat
      721 aaaaaaggaa atatcatcaa tatgtcttct gttcatgaac aaatcccatg gccaacgttt
      781 gctcattacg ctgcttcaaa aggcggagta aaactattca ccgaaacggt agcaatggag
      841 tacgcaccaa agggaatcag aatcaatagt ataggacctg gtgctatccg tacacctatt
      901 aatgcggcaa aatttgatga tcctgaacaa aaagaactct tggaaagtat gatcccacta
      961 ggacgcgtag gtgaaccaaa agaaatagcg gcgacagctg catggttggc ttcagacgaa
     1021 tccagttatg ttacaggtat tacactattt gcagatggtg ggatgacttt atatccttct
     1081 ttccaaggtg gaaaaggata agggtgaata aaaatgggtt tattgattgc attgatcccg
     1141 gcagttgggt gggggatcca gccactgatt ttgaaaaaga ttggtggcag accaacaaat
     1201 gagatattag gaactggtat tggagccgtg ctcgttggct tacttgttca attattcgct
     1261 tctccagctg gtatttcagt gactacattt ttgattagtt tactttctgg tgcattctgg
     1321 gtcatcggac aaacaggaca gtatacatct ttcaacttga ttggtgtttc taaaacgatg
     1381 ccaatttcga ctgcattaca gttagtcgga acctcattga ttggtgtctt cgcttttgga
     1441 gaatggtctg gaagtatggg aaaaatcatt ggtgcagcag caatcattct tcttgtattt
     1501 ggttctgctt taacggcaat cagtgatgat cctggaaaaa aaggcggtat cacaaaagga
     1561 atcagtctat tagcatcaac aagtattggt tattgggttt acagtgcatt gcccaaacta
     1621 gttgatgcaa gcggactttc cattttcttt ccgcaaatga tcggtgtctt tttaggggca
     1681 gtgatttatg tgattttccg aaaaccaacc gcattttctg aagacaagag ttggaaagca
     1741 acaatagttg gagccatctt cagtgtgtca gcattggctt atattttttc agcaaatgaa
     1801 aatggggttg ctactgcata tatcattaca cagctaaacg tagttatctc tacattaggc
     1861 ggtatgtaca tcctgcatga aaataagaca ccaaaagaaa aaaaattaac gattatcgga
     1921 ttattgctga tcgtagtagg aagcatggtc acggtcttta tttgaggata aagatggtaa
     1981 aatctcattt attaaaaaaa tatttgtttg aaccaaacag taagtactta taaaaaaata
     2041 gcgctagaat ttccaacatc tctggaattt ctagcgcttt ttaattgttt aagatacaag
     2101 tgaaatatct gtctgcatca ataatttttc attgatccat tgactaaagt ttacgacata
     2161 attgaagtta ccaccgatta gtatctccgc atgattgtct ttataaagat cgtattgtaa
     2221 aagacggtct tttttatttg cgtaaacatc gttatggtac tggactaatt gttctcggaa
     2281 ttgattgatt gctgtaaaat cacgatactg aaggttgaaa cgcatcgtga ttggtgcatt
     2341 tagcatgaat cgctgcattt cttttgctgc ttcctctgct tgatctccgt ataggaactc
     2401 aaaaagatta cgttcagaaa atttccattg cccgccaacc tttgttcctt ttagttttcc
     2461 ttcatgaaga taatttctga tagaacgtgt agtaacacct aattgttcgg ctaattcatt
     2521 tactgagtaa aaatctttca atgttcaccc tcctatgtga atggttctac tgacttagtt
     2581 gatatttgct taatcacgaa atgtagaaag aacgatttta attaaggtca ttgtaaaaat
     2641 caagtcagaa aattctaact tttctttctc taaa
//
LOCUS       DAKBVG010000245         2624 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000245 DAKBVG010000000
VERSION     DAKBVG010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.247"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            5..1150
                     /locus_tag="OUD09_002518"
     CDS             5..1150
                     /locus_tag="OUD09_002518"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HCT9693744.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1321..1779
                     /locus_tag="OUD09_002519"
     CDS             1321..1779
                     /locus_tag="OUD09_002519"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCT9693745.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..2408
                     /locus_tag="OUD09_002520"
     CDS             1938..2408
                     /locus_tag="OUD09_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HCT9693746.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(2461..2576)
                     /gene="rrf"
                     /locus_tag="OUD09_002521"
     rRNA            complement(2461..2576)
                     /gene="rrf"
                     /locus_tag="OUD09_002521"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          859 a          504 c          494 g          767 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgtcagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccat aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttctttaact taataaaaat aaaaaaagat gaactatttc tagttcatct ttttgccttt
     2461 gcgtggcgac gtcctactct cacaaggggc accccctcac tacaatcggc gctaagaagc
     2521 ttaacttctg tgttcggcat ggttacaggt gtatccttct cgctatcgcc accacactgt
     2581 ggtgttatct tttattgagt aaattttgtt cactcaaaac tgga
//
LOCUS       DAKBVG010000246         2617 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000246 DAKBVG010000000
VERSION     DAKBVG010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.248"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(<1..198)
                     /locus_tag="OUD09_002522"
     CDS             complement(<1..198)
                     /locus_tag="OUD09_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693747.1"
                     /translation="MASWLIEEIENERRKIMDAGITVMLDKQQTNQLKNYVFEMTKEA
                     IDQARIDTGLERPFLKGKEMAK"
     gene            complement(210..410)
                     /locus_tag="OUD09_002523"
     CDS             complement(210..410)
                     /locus_tag="OUD09_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCT9693748.1"
                     /translation="MITLNDQFIRSLRRHRADLILTKNDAAKLIGINRKTYVKIENGS
                     KESIRASTYQKLVNWLLNDLKI"
     gene            533..1135
                     /locus_tag="OUD09_002524"
     CDS             533..1135
                     /locus_tag="OUD09_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCT9693749.1"
                     /translation="MEFTIGGVTLNRIKFYIDSSKKTIKQISTETGINYTTLTNYYRG
                     ERNPRNTETWNILADYFHVPVSYLMGLDETNSIEQPNLDRIKTARLEKKDKIVKNIAS
                     LDSDYFTELSNLYFSMTSLLEVKLQEDDSDFLFLKSILEDSFEYTILFAKQKKFINKA
                     LNLNIDERKELESELFGNLTQVQVNNLKREITARLISKLQ"
     gene            1190..2356
                     /locus_tag="OUD09_002525"
     CDS             1190..2356
                     /locus_tag="OUD09_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCT9693750.1"
                     /translation="MSIQKYTTKSGARYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDTGYHKPSNDLFITVYEEWLPSYRNRVQETTYQRTKDLFRLHILPKFG
                     NKSISKITPVHCQNAVNEWALKFKNIKQLKSYSSQIFEYAIFAELLHRNPMANTNLPK
                     REKKENENYFSLEELKAFWDILNKEEPLKHILIFQLLITTGIRKGELAALHWSDIYFE
                     EQLMYVRKSYATIRNEEKNARRKTVRIQKNTKNISSERILPIDAQTIEMLKIWKKEQA
                     RELLQFGINTNTKNQLIFTYVNADGDINQPLHADYSNNILKRLEKKYKFKHVTIHGFR
                     HTHATLLLEGGASIKETQDRLGHKNAETTLNTYSHVTEKAQRNALEKFIAYTGL"
BASE COUNT          933 a          432 c          396 g          856 t
ORIGIN      
        1 tttagccatc tcttttcctt ttagaaatgg tctttctaat ccggtatcaa ttcttgcttg
       61 atcaatagcc tcttttgtca tttcaaaaac ataatttttc aattgattcg tttgttgttt
      121 atctaacatt actgtaattc ctgcatccat tatttttctc ctctcatttt ctatttcttc
      181 aatcagccaa ctcgccaagt tgtatttatt tagattttca aatcattaag taaccaattt
      241 accaactttt gataagtaga tgctctaata ctttctttgc tcccattttc gattttgaca
      301 tatgttttcc gatttatacc tatcaatttt gctgcatcgt ttttagtaag tataagatcg
      361 gctctatgtc ttcttaagct tctaatgaat tgatcattaa gagttatcat tcagcgcctc
      421 ctttcaaatt attcactttg tggtttttat cttaataagc acattttaaa ccacaaaacg
      481 agtaatatca attaaaatat tactcaattt gtggttttca tgctataata gtttggaatt
      541 tactataggg ggcgttactt tgaatagaat aaaattttat attgattcca gtaaaaagac
      601 cattaaacaa attagtacag aaactggtat taactataca actcttacga actattaccg
      661 tggtgagcga aatcctagaa atactgaaac atggaatatc cttgctgatt atttccatgt
      721 tcctgtaagt tatttgatgg gattagacga aacaaattcc atagaacaac ctaatcttga
      781 tagaataaaa actgctcgtt tagaaaaaaa ggacaaaata gtcaagaata ttgccagtct
      841 tgactcagat tattttactg aattatctaa tctatacttt tccatgactt ctttacttga
      901 agtcaaactt caagaagatg actccgactt tcttttttta aaaagtattc tagaggactc
      961 ctttgaatac acgattctat ttgccaaaca aaagaagttt attaataaag ctttaaactt
     1021 aaatatcgat gaaagaaaag agttagaatc tgagttattt ggcaacttaa cccaagtaca
     1081 ggttaacaat ctaaaaagag agataacagc tcgattaata tcaaaacttc aataaaacta
     1141 attattcaat cagccaactc gccaagttat cagattggag agattagtta tgtcaataca
     1201 aaagtacaca acaaaaagcg gtgcacgata tagtgtccgc ttgtatgttc gtaaagatga
     1261 aaatggaaag caagaatact atgtaaaaaa agggtttaaa actgaaaaag aagccaagtt
     1321 acacgaagca agaaaaaagg tcgagattga agataccggc tatcataagc cttctaatga
     1381 cttgtttata acagtgtatg aagaatggtt gccttcttat aggaatcgtg tacaagaaac
     1441 gacatatcaa cgtacaaaag accttttccg cttgcatata ctaccaaaat tcgggaataa
     1501 gtccatttct aagattactc ctgttcactg tcagaatgca gtcaatgaat gggctttaaa
     1561 attcaagaat atcaaacagc ttaagtctta cagctctcaa atatttgagt atgccatttt
     1621 tgcagagcta ttacaccgaa atcctatggc aaatacaaat ctaccaaagc gggaaaagaa
     1681 agaaaatgaa aattactttt cattagaaga actaaaagct ttttgggata ttctcaataa
     1741 agaagagcct ttaaaacata ttttaatctt ccagcttttg attactactg gtatacgtaa
     1801 aggagagcta gctgctcttc attggtctga catttatttt gaagaacagc taatgtatgt
     1861 tagaaagagc tatgcaacta ttcgaaatga agagaagaat gcaagaagaa aaactgtcag
     1921 gattcaaaaa aatacaaaaa atatatcttc cgaacgaata ttaccaattg atgcacaaac
     1981 tattgaaatg ttaaaaatct ggaaaaaaga acaagctcga gagcttttgc agtttggcat
     2041 aaatacaaat acaaaaaatc aacttatctt tacctatgta aatgcagacg gtgacattaa
     2101 tcaacctttg catgcggact attcaaataa tatattgaaa cgtttagaga agaaatataa
     2161 atttaagcac gttactattc atggattccg gcacactcac gctactcttc tactagaagg
     2221 tggagcaagt atcaaagaaa cacaagatcg attgggtcat aaaaatgctg aaactaccct
     2281 taacacttat tctcatgtta ctgaaaaagc tcaacgaaat gcgttagaaa aattcattgc
     2341 ttacactggt ttataatatt gtcattacct ttctcattac cagaacgaaa aaaagacacc
     2401 ttctcgcaat gagaaagtgt ctgaaatcgt tggtataaca acgattaacg ttttgaaaac
     2461 tgtgaagctt tacgtgcttt cttaagacct ggttttttct tcgtatcatt gaataagttt
     2521 gaaaagcctt gatttataaa ggtttttccg taaatatatt ttgttatatt tgattaaatt
     2581 tgaaatgttt gtgttctaaa atgtgttcta atttaaa
//
LOCUS       DAKBVG010000247         2601 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000247 DAKBVG010000000
VERSION     DAKBVG010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.249"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            47..484
                     /locus_tag="OUD09_002526"
     CDS             47..484
                     /locus_tag="OUD09_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693751.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGQWFFQALLCLEAGVKPNTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYLENVNKYGACYIMKKEDNENE"
     gene            477..1172
                     /locus_tag="OUD09_002527"
     CDS             477..1172
                     /locus_tag="OUD09_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693752.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEELNSKDATVDDYYLVFTKFLTCGTE
                     SIVLMGMDYELAVPEVFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNELDRK
                     RMPYQRFERKKGQ"
     gene            1553..1732
                     /locus_tag="OUD09_002528"
                     /pseudo
     CDS             1553..1732
                     /locus_tag="OUD09_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1794..1978
                     /locus_tag="OUD09_002529"
                     /pseudo
     CDS             1794..1978
                     /locus_tag="OUD09_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358324.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
     gene            2012..2443
                     /locus_tag="OUD09_002530"
     CDS             2012..2443
                     /locus_tag="OUD09_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCT9693753.1"
                     /translation="MLSEPLAKTYPAIFSPEEGGGYFIEFPDVQGAYTGINEDDISYG
                     IAMAQEVLGMVLADYIEHEDLLPEPTPINKISVEDDSFTTLIRVDVAKYLKDTELVKK
                     TLTIPQWADKLGKRAGINFSVLLTESIADKADNILRSGRNN"
BASE COUNT          937 a          405 c          535 g          724 t
ORIGIN      
        1 cgaatattgg ttaaccaaat tagagaacta aaaaggagaa aaaaatatgg ctcaaaatta
       61 ctatgacgaa tttgtcaaat tacctttgga taaaatggca caaaaaatgg aggatatgac
      121 attcctatac aatgagacac gtgttccaaa aaaacattat aaggaaagat tgtcggtagc
      181 tgtggaagaa atgattgaat cgggtgtcga aatgaatctc attgcgacct actatcgaac
      241 gcttgaagaa ctgaaaaagc aaaatggaca atggtttttc caagccttac tatgcttgga
      301 agcaggcgtc aagccaaaca ctatcaaacc ttctgaatac caagctttag aactaacgta
      361 tgggaaattt gtcgaaacga aaaaagccaa gactgtttca agtgaatggt tggactactt
      421 agaaaatgtc aacaagtatg gcgcatgtta catcatgaaa aaggaggaca acgaaaatga
      481 ataaggtgat ttatcaacta acaacggaag atgtgtcaca ctttttaaca aacaaagaac
      541 atttgaatga agtaatcaga cggattcaaa aagactttag ttgtatggaa gaaatgaagt
      601 actttattca aagagtcgaa gaacaattgt atttggaaag tgaaacgagt caaacgaagg
      661 ttacagtcag caataattat ttagacgtgt ataaacgtcg tcctgacaca ttaaaaaaag
      721 gagcggagct tctacgacta gaaacagaaa aatatatcat gaatgtttac ctagaaagga
      781 gtgaactggt cgaaaacttt gtcagctaca cgatttgcag gaaacactat tactcagaat
      841 cagaatatta ttcaattttt gagagcatgg aattaatgga agagctaaac agcaaagatg
      901 cgacggtaga cgattattac cttgtattta ctaaattttt gacgtgtggg acggaatcta
      961 tcgtgttaat gggaatggat tatgaactcg ctgtacccga agtatttttg agggaatatg
     1021 tagaagaagt agataaccca ggcgacgact ggtacacgac tttaactgat tttttagata
     1081 cctacactta cgacgataca gaagaaatcc aaaatgaact tgaccgtaaa cgaatgccat
     1141 atcaacgatt tgaaaggaaa aaaggtcaat aaccatttat accgaaaagt gagaatgtgt
     1201 gaaataaaaa caaaaaacct tgaaattaag ggaatggttc ttatttcgca ccaatcgctc
     1261 caaccgcccc cgccgcttag gtggtgcgaa ataagcgagc aaagcccgaa aacctaagag
     1321 aaagaaggag atttatatga acaaaattat tcccttatta ttagtcctat ttgttggaga
     1381 aatacttatg cgacttagtg cagctgtaca tttaaacctc gcagtatttt tggtaatact
     1441 aatgttttgt ttgatctttt ggcgatttgt attagccctc gtaagtctac tattcaagat
     1501 gattgcagcc atatttttca taggattatt tcgatgaaag gagagagtac caatgccaat
     1561 tgcaatagga aataaacggt tacccgtgac gttggatgaa aagagacaaa aagaattgca
     1621 gtaaccaaag cagaagtacg gcaaaagtga atccaggatt atgtgtattg cgttagattt
     1681 attgattgcg caagaaaaag caggatttga cataccagca ctcaaaaagt gatcgcactt
     1741 tgcgcacgtg ttaatacgtg ttataattat tttgtaagga ggtatagtgc acaatgccac
     1801 ttacgggaaa agaaatgctt aagttattga aaaaaatggc tgggttgagc gaaggcaaga
     1861 gggatttcat catcatcttt ataaggatgg tgttagaatc actgttcctg ttcatgccaa
     1921 tcaagatctt ggccgaggac ttgagcgaaa aattttaaag gatgcgggat tgaaataatc
     1981 tcgcattcga ttaaagaaag gattgactga aatgttaagt gagcctttag caaaaaccta
     2041 tcctgctatt tttagcccag aagaaggtgg tgggtacttt attgaatttc ctgatgttca
     2101 aggtgcttac acaggaatta acgaagacga tatatcttat ggaattgcta tggcacaaga
     2161 ggtgttaggt atggttttag cagattatat tgagcatgaa gatctacttc cagaaccaac
     2221 accaatcaat aagatttctg ttgaagatga ttcttttact actttaattc gtgtagatgt
     2281 tgccaaatat ttgaaagaca cagaacttgt caaaaaaacc ttaacaattc ctcaatgggc
     2341 ggataagcta ggcaaaaggg ctggcataaa tttttcagtc cttctaacag aatcaattgc
     2401 agataaagca gataacattc ttcgttctgg aagaaataat tagaactcaa aagggtcaga
     2461 aacttaaaaa tctttggctt ctttttttac tacaaagatg gcttctttaa attatttgcc
     2521 tgattgattt aaaaaaattg cgcaaagact acgagctaat taagttgctc gtggtctttt
     2581 tttatgaaaa ctttttagat c
//
LOCUS       DAKBVG010000248         2577 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000248 DAKBVG010000000
VERSION     DAKBVG010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.250"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            14..>2577
                     /locus_tag="OUD09_002531"
     CDS             14..>2577
                     /locus_tag="OUD09_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693754.1"
                     /translation="MNQYVKKLIASILFVFPLLMVTSVFADVEKTSESTQSENQSFDK
                     TTFSEESITSETTESQSEAPTSNTRQEENKTSETSEVAEQTSDSSEEVQVRVVSADGY
                     SETATTEELTQLLADESVAKIRLTQPLTLDRELEIKRDIVIDFGGLAHNFGTHHIYIN
                     ETPQSIEFQNFKGTAAHPGGLIPNIDGNAIIIAYMSDFWGNRYHFTGEIKFTGTLDLY
                     DGSKLGLIYAPRATVTLDGVSGVLDVQPVKEGMNAAPGKAYFCRSYQLNVINGSQLYG
                     PYLGKFYGFLDSGDETGSGKTAPGLHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVT
                     FKVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSRVAVSNDGKITINTATTGGFRLQG
                     DSSQIHVSSGGQISVKMAGDGDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSA
                     VRFENGTQKLTVTNGGSLKVRNAGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFT
                     VTGEKSNIDIRADSGATVDTTGDIDLNFMAGEKTYLVMVGKTGEDKTGIISGDVLNVD
                     LESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKAGVTFDNFEKDP
                     DYFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRV
                     PTNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSPVEITALTQGQTDE
                     NEGTGYNPWGEGEQGGLFEVQVPNEERLTTGDQVQIVAAKKVRGGQEVTELDSAKTTV
                     DVIPPEPAKLSTTLINAASRSITGTGEAGALVSLKKNQAVLSETTIDDTGKFVLSIP"
BASE COUNT          827 a          468 c          620 g          662 t
ORIGIN      
        1 aggatggtgg agaatgaatc aatatgtaaa aaagctgatt gcgtcgattt tgtttgtctt
       61 ccctctcttg atggtgacat ccgtttttgc cgatgtagag aaaacaagcg aatcaacgca
      121 atcagagaat caaagttttg acaagactac tttttcagaa gaatcaatta cttctgaaac
      181 tacagaatcc caaagtgagg cgcctacttc taatacaagg caagaagaaa ataagacaag
      241 tgagacctca gaagtagctg aacaaacgtc tgatagcagt gaagaggtac aggttagagt
      301 tgtttcagcg gatgggtaca gcgaaactgc cacaacagaa gaactgacgc agcttctcgc
      361 agacgaatca gtagctaaga ttcgtttaac tcagcctttg actttagaca gggaattgga
      421 gatcaagcga gatatcgtta tcgattttgg cggacttgct cataatttcg gcacacatca
      481 catctatatc aatgagacac ctcagtcgat cgagtttcag aattttaaag gaacggcagc
      541 tcatccaggt gggttgatac caaatatcga tggaaatgcc attatcattg cttatatgtc
      601 cgatttttgg ggaaacagat atcattttac gggagaaata aaatttaccg ggacacttga
      661 cttatatgat ggttctaaat taggcctgat ttacgcccct cgtgcaactg tcacattaga
      721 tggtgtttcc ggtgtattgg atgtccaacc agtgaaagaa gggatgaatg ccgctccggg
      781 taaagcttat ttttgtagaa gctatcagct aaatgtaatc aatggtagtc agctttatgg
      841 accatatcta ggaaaattct atggttttct agattcaggt gatgagacgg gcagtggaaa
      901 aacagcacca ggactccata tcttgagtgg ttcaaaggtc tctcttgact atgatcgggc
      961 agatggcgtc agcgatgaag gagaggctgt cgatacgcta cccgggaatg tcacatttaa
     1021 agtcagtggc tcaggaagtg aattttcggt aaatacaaag atcaatatta ccaacgacaa
     1081 caatcgcgga attatccaaa tgagaggctc aggatcaaga gtcgctgtgt cgaatgatgg
     1141 gaaaatcaca atcaatacag cgactactgg cggatttcgc ctgcaaggag attctagtca
     1201 aatccatgta tcaagtggtg gacaaatctc ggtcaaaatg gctggagatg gcgaccaacc
     1261 tggaaacaat gggatgcgtt ttgtagggaa aggactttct ttgatcgtag aaggagccgg
     1321 atcagtcatc gatattgata agcaaagtgg tcgttctagc gcagttcgtt ttgagaacgg
     1381 tacgcaaaaa ctaacagtaa ctaatggagg ctcattgaag gtaagaaatg caggggatgg
     1441 caaatcttat gtgaacagac tcaatcaagg aaaccaagcg atccaatttt atgacgcttc
     1501 tggttttcta gaaagtccag ggtcagctga ttttacggtt actggagaaa aatcaaatat
     1561 cgatatacgc gcagattccg gagctaccgt agatacaact ggagatattg atttgaactt
     1621 tatggctggc gaaaaaactt atttagtcat ggtcgggaaa actggggaag ataagactgg
     1681 catcatcagt ggggatgtgt tgaatgtaga cttagaatct cctacctatt tcgatttcca
     1741 aaacaaacgg atcggaagcg agactaatgg cataggtggc tgggtatttc aaggaaatgg
     1801 cgattcttct ttgaagatcg ctcattcaga aattgcttta tggcgaaagg caggagtaac
     1861 gttcgataac tttgaaaaag atccggatta tttttctgat atgacagact tatcgatcaa
     1921 tggaaccgac ttaggtaatt ttgtatcaag ccctagtgca ggtttaacaa atgaatttgc
     1981 tcagacaggt gccggaatga aaaattataa tcgaatcagt gccaataacc agcttccgat
     2041 gattgaatcc ttaagagtac caacgaatgc agataaaaaa atctatgggc atgtcgttgt
     2101 tcccgaaggt gtcgaggcaa agccaagaga tgcttggaca ggcgaagtag aagtgacttt
     2161 acgtattact tatgcagata cgacgaaatc acctgtagaa attactgctc tcacacaagg
     2221 acaaacagat gaaaatgaag ggacaggata taatccgtgg ggagaaggcg aacaaggcgg
     2281 tttgtttgag gttcaagtac ccaacgaaga aagactaaca acgggggatc aagtgcaaat
     2341 agttgctgct aaaaaagtaa gaggcggcca agaagtgaca gaactggact ctgccaaaac
     2401 gacagtcgat gtgattcctc ctgagcctgc taaattaagt accacattga tcaatgcagc
     2461 ttctcgctca atcacaggta caggcgaagc aggagctttg gtttcattga aaaaaaatca
     2521 agctgttttg tccgaaacaa ctattgatga tacaggtaaa tttgttcttt ctatccc
//
LOCUS       DAKBVG010000249         2563 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000249 DAKBVG010000000
VERSION     DAKBVG010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.251"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(132..710)
                     /locus_tag="OUD09_002532"
     CDS             complement(132..710)
                     /locus_tag="OUD09_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCT9693755.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            complement(730..1398)
                     /locus_tag="OUD09_002533"
     CDS             complement(730..1398)
                     /locus_tag="OUD09_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT9693756.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            complement(1413..2483)
                     /locus_tag="OUD09_002534"
     CDS             complement(1413..2483)
                     /locus_tag="OUD09_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT9693757.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
BASE COUNT          745 a          558 c          391 g          869 t
ORIGIN      
        1 taccctgata atcatgagag aataacaaat ttgtatttat gaaaaactac ctatataaaa
       61 aaaacaagat ataacgatct tcgtcatatc ttgcttcttc acctaactat ttttctccct
      121 atataacagc tttacgcttc tcctaataca ccattcaaat ataaatcgat caattgcgcc
      181 acatttagcg gttcttccac aggtcttttc gaccgagtga tgtaaggtgc taacactaag
      241 aaaaaatgcc ttgctgcgat ttcttttgaa atcccatttc ttagcacaga ttggtttgct
      301 gaaaagattt cttcaaaagg ttcacgataa tttttcttcc atagaaagaa aagatgatct
      361 cttgtttcct ttgaaagatt gctttcaaga tcgtgaagca tcagaaacaa atcgaccaga
      421 tgtgtctcaa tcaaaaactg cgccattttt gtcaaggtta cttgaaatcc cgtttcgctt
      481 gtttgaacga tttctctcaa agcttttcca acatctttta agtggttttc taaaactgcc
      541 gtatatagtt tttctttatc accgaaataa tgatagagat tgggttgggt gatcccaact
      601 ttttgtgcga tctctcttgt tgaagtgccg agatatccct tggacataaa caagtcagtc
      661 gcttctttca aaattttttg ctgtgtatct ttgttgtatc gatggaccat ttttccacct
      721 gctttttggt tattcttttt ctttcaatac accatcttcc atcataaata ttctatcgca
      781 tttgtcaatc agccgtgtat cgtgagtaac catgatcgtt gctttatttt tgtcttttga
      841 ttcttttgcc aagatatcga ctacttctcg tgccttttct gaatccaaac tagcagttgg
      901 ttcatctgct agaataatgg acggatcatt gtacaatgca cgagcgattg cgaccctttg
      961 ccgttctcca ccagatagtt cttctggata cttgttgatc aacttatcca cgccaagctg
     1021 ctcaaataat tcttttgctt ctttttgacg atgttcatgt ttgatcttat ctactaataa
     1081 taactgcttt tttactgtca gaaaaggaac cagattggaa gcttgcaaaa taaagccaat
     1141 ttctttaaaa cgtagtttaa cgcgttcact ttctttttga ttactgaatt cttgatcatt
     1201 gataaataca gagccactgc taggcgtctg caatccgcct gccaaagtca aaaaagtact
     1261 tttacctgaa ccagaaggac cgatgattgc aacaaattcc cctttatcga tactcatggt
     1321 tgtttctttc aatgctttaa ctacttggtc gccatcttgg aaactttttt ctacttcttt
     1381 aaattctatt gctttcatgc tcttcctcct tcttatccaa ttgccttcaa cggatcgatt
     1441 tttacgattg tccgaacaga aaatagtgcg cctaacacag ctactaccag catcaacaag
     1501 ctaatgacgc caaaaaacag ccaattgctt tggaatggaa ccgcatcagg tagaacaagg
     1561 gatgttccca ctgttcctaa tagaccaaga agaataccaa caaatgataa aatgaatgtt
     1621 tgtgccacta cagaacgcgc aataaatcct cctgtgatcc cttgtgcttt cattacacca
     1681 aagatattaa tcttttgcat cgtcagtaca taaatgaaaa tcccgatcac aatagccgca
     1741 ataacgatca aaaatccgat cataaatcca aaagtcaata cttgcgcatt atatccaggt
     1801 aattcattga tgaatttaga aattttgatc tgttctaaat cttcaggtaa attactgatt
     1861 ttacctctga caactattgc gttaatccgt gcattttcac ttgtatcttc tttttcgaag
     1921 cggatctctt ggtaagcatt gatggttgta tagagaactg gtgagacatt gaatttggca
     1981 tgatccgtaa agccagttag tttaaacgtc ttgtcactac cagataactt aactgtatct
     2041 cctacggcta gaccatattc ttctttcaaa ctaatatcgc ctactgcctc atcatcgtta
     2101 tcaaaagctt ttccttcaac aagattgggc atgatgaatt gatttttatc gataccgaag
     2161 aagctaacat tgatttttcc ttcttctgta ctgtctttac tagtcacgac tccaggtgtc
     2221 tgggcaagat acgccacttt cccaccttcg acttcttctg ctatcttctt agtaatcatg
     2281 gacatcccta gatttgaatt tgcatctttc gaaagcacaa tcgaatctgc ctcccacttg
     2341 tcgactgctg ttcgattatc ttgagctaag ccataagcta acccagtcaa aaaaaatacc
     2401 aggtaggcga tcaaaaacat cacccctgca actaacgcat aacgtaactt tgaatgcata
     2461 atttcattta atgctaaaaa catttaactc ctcctcctga aataatcatt ttatacaaca
     2521 attttatcac ttgataaatt tttggcgaat attatgctca aaa
//
LOCUS       DAKBVG010000250         2556 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000250 DAKBVG010000000
VERSION     DAKBVG010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.252"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            256..2409
                     /locus_tag="OUD09_002535"
     CDS             256..2409
                     /locus_tag="OUD09_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HCT9693758.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          884 a          440 c          554 g          678 t
ORIGIN      
        1 aaaacgtagt tttccacaaa taaatgatcc atcaaagtgt ttcacgactt aagaaaacat
       61 aattttcaaa aataagttta ttttttaatg aaaaaaatag ttggatatgt aactttaagt
      121 gattgctgtg aaacaaaaag ttgattttat cttgaaatta ggtaaagaat ttcttgctcc
      181 gcccattttt tcttttttat taagaagttt ttgggtacaa tgaagcatga atgtaaatag
      241 gcggaaggat aggaaatgat gaaagatttt atgaaaaaaa tattaaatac ttcttggtta
      301 gaaaaaataa gaaaaggtgc acaagaacct aagaacaaaa cgtttcgccg gtcccatgtt
      361 ccttttcgct taaattttct ttttttcatt atatttacgt tatttgttgc gttgattgct
      421 agattgggtt atttgcagat cgtgaacggc gaagagatgg aggcgcgagt gaagtctact
      481 tctacgatta cggtccaaga aagtactcct cgcggaatga tctacgactc cacaggaaaa
      541 gctttagtta cgaataaagc gaatcaggcc atcacatata cgagaggaac acaagttacg
      601 gctaaagatt tgcttgaaat cgcaacgaag ctgaactcgc tgatcgatgt tccggtagat
      661 gaaaatctga cagatagaga taaaaaagat ttttggctgg ctgatcccaa acatttgaaa
      721 gaagccacaa aaagactttc ggcaaaagaa aagcaattag ggaacagcga acaatatagt
      781 gcgatggtca ataaagtcac agctgatgag attaatttaa acgaggagca attaaaaatt
      841 gctaccatct ttaaacggat gaatagtgca tatgaattaa acactgtcta tatcaaaaac
      901 tctggtgtta ctgatcaaga gttagctgtt gtggcagaac gcgcttctga acttccagga
      961 gtttcgactg gcacagactg gacacgagaa tataatgcag caagttcact gaaaagtatt
     1021 cttggcagtg tgacgactga aaagcaagga cttccagcag atgaagcaga gaaataccta
     1081 gcaaaaggtt attctcggaa tgaccgtgta ggacaaagtt atttggaaaa acaatatgaa
     1141 gatgtcctgc aaggaaccaa aacccaatat gaagtttccc ttgataatga aggaaatgtt
     1201 tcgaatcaaa aagaaatctt ctctggggaa aaaggttcta atttaatgtt atctatgaat
     1261 gcagaattcc aatcaaaagt agaagaaatt ttgaaaagga attatcaaac attgatcaac
     1321 aacggcaaag cacagtattc gcctggtgct tatgcagtgg ctatgaatcc tcagacagga
     1381 gaagtgctag caatgactgg tttcagtcat gaacaaggtt ccaaagaaat caccgagaat
     1441 gctttaggca cgattactag cgcctttgat ccagggtctg tagtcaaggc tggtactttg
     1501 acagcaggct gggcaagtaa cgcgatcagc ggaaatcaag ttttgattga tgagccgatt
     1561 cgtctgcaag gtgcttcaga aaaaagctct gtctttaatc gaagcggaca agttgctctt
     1621 gatgcagtca aagcatcgga attgtcttct aatacgtata tgatcaaagt ggcgttaaag
     1681 atgttaggac tagactatac acctggaatg ggactaccta gtttagatga agaagctaaa
     1741 gcttatcagc aattgagaga ctctttcaaa gagtttggtc ttggtacaac gactgggatc
     1801 gatttgccga atgaatctcc tgggatctca cgttctgttg attatatgaa gaagttcaat
     1861 gcggataatg gcaaagaatg gtatgctcca ggtaacttta ccgatttggc atttggacag
     1921 tttgacacat atacaccaat ccaattagct caatatgctt caacagtcgc taatggcggg
     1981 aaacgagtac agccgcacct agtcaaagcg atctatggca atgacgaaaa tggaaacttg
     2041 ggtgaagtga agaaagaaat tggcacaaca gtagaaaata cggtcaatat ttctgcagaa
     2101 aatatggcga tcctccgtga aggattccac caagttgtcc atggtaccga tccttacaca
     2161 acagccaaac cattagccag tgcgaaaatg gacctttccg caaaaacagg gactgcagaa
     2221 acggttgcgg aaggtcatcc agatattgcg acagtcaata gtaatatcgt ggcatacgga
     2281 ccaacagaca atccggaaat tgccatcagt gttgtcttgc cgaacttatt agacgaacaa
     2341 gaccacataa acttgacgat tgctaaagaa ataatggacg cgtattatga tatgttcatg
     2401 gcaaaataaa aaagaggtcg tgaaaaagcc gcttagtttc gagtatgaag aaaaattttg
     2461 tgaaaatagc ttctcacatt tttgcttaaa tttgacttca taccgagaaa ctggcttttg
     2521 aacaccgttt actcggcaaa aaaggttgtg acaaaa
//
LOCUS       DAKBVG010000251         2549 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000251 DAKBVG010000000
VERSION     DAKBVG010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.253"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(45..1022)
                     /locus_tag="OUD09_002536"
     CDS             complement(45..1022)
                     /locus_tag="OUD09_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HCT9693759.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            complement(1025..2134)
                     /gene="pdhA"
                     /locus_tag="OUD09_002537"
     CDS             complement(1025..2134)
                     /gene="pdhA"
                     /locus_tag="OUD09_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HCT9693760.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
BASE COUNT          694 a          533 c          473 g          849 t
ORIGIN      
        1 aatttaaatt gataagccat gactttgttt cgtcccttct tttcttaaaa ttccgcgatt
       61 tcttttactt tgtcttcgat atcttttgca tttggcaacc agacattttc tgcttgtcca
      121 aatgggaaga ctgtatcagg tgcagaaact cgtccgattg gggcttctaa tgaaaggata
      181 gcgcgttctg agatctcaga aactacttgc gcggcaacgc ccgcttgtcg ttgtgcttct
      241 tggacaacaa ctacgcgacc agttttttcc acggattgaa tgatcgtttc tacatctaat
      301 ggcgcaactg tacgaagatc aatgatttca acagatatat tttcttttgc aagattgtct
      361 gctgctttga ttgcttcacg caccattgct ccgtatgtga taatagatac atctgttccc
      421 tcacgagtaa ctgctgcttt atccaaagga acttcgtaag cttcatccgg cacttcttct
      481 cggaatgagc gatatagttt catgtgttct aggaaaacaa ccggatcgtt acttctaata
      541 gaagaaatca atagtccttt tgcatcataa gggttggaag gaatcactac tcgaatacca
      601 ggagactgag cgatcaaacc ttctaaatta tccgaatgca attctggtgt atgaacaccg
      661 ccaccaaatg gcgaacgaat tgtgatgggt agatttctag tgccgctcat tcgatatctt
      721 gtacgagcca tttgagctac tacttcgtcc attgcttcga aaataaaacc aaagaattgg
      781 atttcaggaa ctggacgaaa tccttctaac gcaagaccga aactcaatcc tgcgattcca
      841 gattcagcta aaggagtatc gaatactcgg tcttctccga acttttcctg caatccttca
      901 gttgcccgga aaactcctcc gtttttccca acatcttcac caaaaacaac gacattctcg
      961 tcattttcta gttcaagtgc taaggcatct gtgattgctt ggatcattgt tttttgtgcc
     1021 atgattattt cgactccttc gcttcataaa ttgcaatttg ttctttgatt gattgtggag
     1081 atacttcgaa catgtttttc aagaaatctg aaaccttttg ttttggtact ttgtcggctt
     1141 cagcaatagc tgctttgatt tcttctttag ttgcttcgat gacttgttct tctttttctt
     1201 cagaccaaag tcctttttcc gtcaaatatt tacggaagcg gatcaatgga tcttttttct
     1261 gccaatcgtc atctgtgtct tttgtacggt agcgggttgg atcgtcacca gatagtgtat
     1321 gtggtccata acgataagtc agtgtttcga ttaggactgg tccatttcca ctgatcgccc
     1381 attcccgtgc cattttagat acagtatata ctgcaagcgg atccattcca tcgacttgaa
     1441 taccaggaat cccagctgcg acagcttttt gtgcaagtgt ttttgctgca gtttgttttt
     1501 cacgaggtgt agagatcgca tacccgttgt tttggatata aaatacggca tttgcatggt
     1561 aagcgccagc gaaattgatt gcttcgtaaa aatcaccttg tgatgaaccg ccatcaccag
     1621 tataagtgaa gacgacattt ttcttgtttc gtttcttcaa acctaatgca acaccggctg
     1681 cttgtacata ttgtgcaccg atgataattt gtggtggtag agctttcaat tcttccggat
     1741 aagaattacc tgcagcatgt cctcgagacc ataaaaaagc ttgagataaa ggtaaaccat
     1801 gttttactag ttgaggtaca tcccgataac ctggaagaag aacatcttct ttatcaaatg
     1861 cgtaagcact agccagctga cttgcctctt gtccagcagt tggtgcaaaa aagcctaggc
     1921 gtccttggcg gttcagcgct gttgaacgtt ggtctaatac acgagaccat accatatgag
     1981 tcattaattc aacgagttca tcatcggata aatcaggtac taaatctttg ttaactacgt
     2041 ttccatcttg atcaagtgct tgatatactg ggaaatctgc attgacttca tctagcagtg
     2101 cttggaagtc gatcggtgct tttttgtttg ccatttgtca cacattcctc tctcatctat
     2161 aagaaaatct cttcattctg cgattgaaac agtgaataaa actgtatcac tatttaaccg
     2221 tcttcattaa taaattatca tggatttatg agaaatacaa gctaaaagca caaattttca
     2281 cgatgaaagg aaattatgcc aatccgcata ttaacagcat tagtgaataa aatcacaata
     2341 aggaaagcgt tttatatttc atctgcattt tttctatttt ctaatcaaac ttacaaccat
     2401 ctgtttctat ttaaaaacgt tggtgttata cagttttgtt gcaaacattt attttattta
     2461 cacatcgtga acggacgtac aaataatggt tttttcttag tttctttata tcgtattctt
     2521 tttccgaaaa aaaagaaaca gcttgaaat
//
LOCUS       DAKBVG010000252         2543 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000252 DAKBVG010000000
VERSION     DAKBVG010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.254"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(96..911)
                     /gene="thiD"
                     /locus_tag="OUD09_002538"
     CDS             complement(96..911)
                     /gene="thiD"
                     /locus_tag="OUD09_002538"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCT9693761.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKDAQQETVNDYVLLESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            1205..1480
                     /locus_tag="OUD09_002539"
     CDS             1205..1480
                     /locus_tag="OUD09_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693762.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            complement(1509..2468)
                     /locus_tag="OUD09_002540"
     CDS             complement(1509..2468)
                     /locus_tag="OUD09_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HCT9693763.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERPVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKSMDFRK
                     VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
BASE COUNT          753 a          484 c          421 g          885 t
ORIGIN      
        1 tcacaagtca caatattcaa caaacatgag aagctgtttt ttgaacttgt tccttgtgac
       61 tcggagctgg acggcaggct cacgacctct ttcttttact ctaattcacc agctgcccaa
      121 tgattgatgg gtccatattt gtgccctaca tttatttctt cacgaattgc acggtttaca
      181 tagtcttttg ccaatcggat cgcttgttca actgtttttc ctttcgctaa ctcagctgca
      241 atacatgcag atagcgaatc gccagttccg ttgatccggt cggtaggata aaatggtgca
      301 tttaaccaaa atgattttcc tgattcgagc aatacgtagt cattgactgt ttcttgctgc
      361 gcatctttag catgatgtcc tttgatcata acgttttttg cacccatatc ttgcagttct
      421 tttgctgctt gcacaaaatc ttcttcagct gctaatgtcc gtccagtcag cttttctgct
      481 tcataaaaat taggtgtcag tacttcggcc aaagggacaa gcttttcttt caagctttgc
      541 aaagcagatt cttctagtaa aagatttcca tgtttcgtta cgattacggg atccacgact
      601 aatggtccaa aatcatattt ttggtaattt tttacaactg tctcaatcaa ctttgaatct
      661 gctagcatac ctgttttagc tgcacgtatt tgaaagtcct tcgcaatcag ttcaaattct
      721 ttgtcgataa aatctgttgg caatgtcaca cttgctccga ttccgtaaga attacctgca
      781 acacatgcgg tcatgacaga aacaccgtaa acatttcttg taaagaatgt atgcatatct
      841 gcttgcatcc ctgcgcttcc atcgctatct gatcctgcga tcgtcattgc ttgtacatac
      901 tgatcttcca tcaaatcgat tcctccattt ttataaaaag atttttcata atgctgttac
      961 tgtttctatc atatgttttt caaagtatcc ctcagtaaat ataccactac cttttcttgg
     1021 taatcaaatt tatttgaaaa aacatttgta ataaatcaga aaatagttgt catttgtttg
     1081 tattcgctat tctaataata aataaccact atttgattta tatatatttc agtatccttc
     1141 ttgttttcat ataaccaaag gcgctatgat ttccatatga aaaaaataaa gtcaggtgaa
     1201 aaaaatgaaa cttgaggata aaaaagtcat tttgaagaaa atcaaacgac taccagacca
     1261 aatagatggc tatacgatct gttctttcaa aaaagacagg agtataaaag cttatacgca
     1321 aaacacagaa aaaggaataa tctatttcct acatgaagaa ggttacgata cccaaacctt
     1381 tatgtttact gaaaaaaaag aatttcataa acagatgaaa aaactgattg aaaaagaatt
     1441 tccaagaagt cataaactct atgtgaatca gcaattttaa cttctaactg ttctttattt
     1501 atgatttgtc aaattaagct agaataaaat gcttcttgta catagctcaa aaatatttcg
     1561 attggtgttc tgtaattcaa tgattttctt ggaatatgat tacgttgatt gctgacactg
     1621 gaaataaatg tctgattcac ttttctaaaa tccattgatt tcggcagtcc attacgacgc
     1681 agaatcccgt tagaattctc gtttaatggg cgttgagaag gtgttccagg gtccgcaaaa
     1741 tatatatcaa tatcatgttg gttactaatg gctttccagt tagaaaattc ttttccacag
     1801 tcaaacgtaa tagatttaaa gaagttttta gggaagcgag aaaaccattg attaagggca
     1861 gtttcaatat ctaatgcctt acggccgttg ggtttaatcg tgataatgac tttagataat
     1921 ctttcaacta aagtaatgac ggcactttta tgatgaatgc caacgatcgt atcaccttca
     1981 aggtgaccaa actcagaatt gaaatcagga taattatcag gtcgatcatg gattgagcgc
     2041 tgatactgtt gttttccccg tttttcctga tggccattgg gttttctttt acctttcatc
     2101 ggtagtgtgt caatatcaaa tattccttta gaaaataaac gataaagtgt tctcataccg
     2161 catgaaacag gcctttcttt tcgcccgata atgacgtcag gcgtccaacc aagagtgact
     2221 ttctctttaa tgtaatctac ttcatgagca ggtaattgaa tgacttttct accacatttt
     2281 ttcttatttt ctttatactg gtgccaataa tcaagagcag tcttgccttg cttgaactta
     2341 ttgactacat tataaatagt ttgtatagca cgtcccattc ggttagcgat ttcaaccggc
     2401 ttattatgtt gaagataata tgattctatc attgtcagtt cgtctatggt aagatgtttg
     2461 taggtcattt atggttacac tcctttgttt tctttcgtcg gaaatacaat ttgagtgtac
     2521 cataaatgcc tttttatttt tct
//
LOCUS       DAKBVG010000253         2541 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000253 DAKBVG010000000
VERSION     DAKBVG010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.255"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            53..2494
                     /locus_tag="OUD09_002541"
     CDS             53..2494
                     /locus_tag="OUD09_002541"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HCT9693764.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKPYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     KVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT          846 a          454 c          603 g          638 t
ORIGIN      
        1 gctatttgtt tttacagcaa agaatggtat atttttgtag gaggtggctt acttgaaaga
       61 agtcgttatg atagatgctg caagaacgcc gataggaaag tatcgcggaa gtttaagtcc
      121 atttactgca gttgaattag gcacacttgt gactaaagga ttattagata aaacaaaact
      181 gaaaaaagat aaaatcgatc aagtaatatt cggaaatgtc ctgcaagctg gtaatggtca
      241 aaatgttgcc agacagattg ctttgaacag cggtctccct gtagatgtcc cagcaatgac
      301 gatcaatgaa gtttgcggat ctggtatgaa agcagtgatc ttagcacgcc agctgatcca
      361 attaggtgaa gcagaacttg tgatagccgg cggaacagag agtatgtctc aagcacctat
      421 gctgaaaccg tatcagtcag aaacaaatga atatggtgaa ccaatttcca gtatggtcaa
      481 cgacggattg actgacgcat tttcaaatgc acatatggga ttaaccgcag agaaggttgc
      541 aacacaattt tctgtgagca gagaagaaca ggatcgctat gccttgtcgt cccagttgaa
      601 agcagcacat gctgtcgaag ccggtgtatt ttctgaggag atcatcccag tcaagatttc
      661 tgatgaagac gtgttatctg aggatgaagc agttcgtgga aatagtacat tggaaaaact
      721 gggcacgtta cgtacagtat tctcagaaga aggaactgta acagcaggaa atgcttcgcc
      781 attgaatgac ggtgcctctg tggtgatcct tgcatccaaa gaatacgcag aaaataataa
      841 tctgccttat ttagcaacca tcaaagaagt agcggaggtc ggtattgacc caagtatcat
      901 gggaatcgct ccaatcaaag ccattcaaaa attgacagat agatcaggaa tgaatttatc
      961 aacaatcgat ctttttgaga tcaatgaagc atttgcagct tcttctatcg tagttagcca
     1021 agaactgcag ttagatgaag aaaaggtcaa tatatatgga ggagcaatcg cattaggcca
     1081 tccaattggt gcaagcggtg cacgtatctt gactacatta gcttacggat tgcttcgtga
     1141 acagaagaga tacggtattg cttcattatg tattggtgga ggtttaggct tggcagtctt
     1201 gttagaagcg aatatggagc aaacacataa ggatgtgcaa aaaaaaaagt tttatcagct
     1261 tacgccatct gaacggcgca gtcaattaat agaaaaaaat gtattgacac aagaaactgc
     1321 gttgattttt caggaacaaa cactttctga agagctctcc gatcacatga tcgaaaacca
     1381 agtgagtgaa gtggaaatcc caatgggcat cgcgcagaat tttcaaatca atggtaagaa
     1441 aaaatggatt ccgatggcaa cagaggaacc atctgtcatt gcagctgcaa gtaatggtgc
     1501 aaaaatctgt ggaaatatct gcgcagaaac acctcagcgg ctgatgcgag ggcaaatcgt
     1561 tctatcagga aaatcggagt atcaagcagt gatcaatgca gttaatcatc gaaaagaaga
     1621 gctgattctt tgtgcaaatg aaagttatcc ttcaatcgtg aaacgcgggg gcggtgttca
     1681 agatatctct actcgggaat tcatggggag tttccatgca tacttatcta ttgatttttt
     1741 agtggatgta aaagatgcaa tgggcgctaa catgatcaac tcgattcttg agagtgtggc
     1801 aaacaagtta cgggagtggt tccctgaaga agagattcta ttcagtattt taagtaattt
     1861 tgctacagaa tcattggctt ctgcctgttg tgaaatccca ttcgaacgct tagggagaaa
     1921 caaagaaatc ggtgaacaaa ttgccaaaaa aatccaacag gcaggggaat atgcgaagct
     1981 tgacccttac cgtgcagcaa cgcacaacaa agggatcatg aatggtatag aagctgtagt
     2041 ggcagctaca ggaaatgata cacgtgcggt tagcgcttcg attcacgcct acgcggcaag
     2101 aaatggatta taccaaggat taacagactg gcagatcaaa ggggataaac tagttggaaa
     2161 actaacggtg cctttggctg ttgccacagt aggcggagct tccaatatcc tgccaaaagc
     2221 aaaagcatct ttagctatgt tagatattga ttcagccaag gaactagcac aagtaattgc
     2281 tgcagtaggg ttagctcaaa acctggctgc tttgcgtgca ttagttacag aaggaatcca
     2341 aaaaggccac atgggacttc aagctcgttc attagcgatc tcaattgggg ctattggtga
     2401 ggaaatcgag caagttgcga aaaaattacg tgaagcagaa aagatgaacc agcagacagc
     2461 tatacaaata ttggaaaaaa tccgggaaaa ataaaaaagc aatatcttta gaagaataaa
     2521 gcaagattta cagttatttt a
//
LOCUS       DAKBVG010000254         2508 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000254 DAKBVG010000000
VERSION     DAKBVG010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.256"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            <1..311
                     /locus_tag="OUD09_002542"
     CDS             <1..311
                     /locus_tag="OUD09_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCT9693765.1"
                     /translation="TSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIGRGLTLL
                     EAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGFTYHPIE
                     "
     gene            411..845
                     /locus_tag="OUD09_002543"
     CDS             411..845
                     /locus_tag="OUD09_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCT9693766.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            1025..1435
                     /locus_tag="OUD09_002544"
     CDS             1025..1435
                     /locus_tag="OUD09_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HCT9693767.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            1492..1932
                     /locus_tag="OUD09_002545"
     CDS             1492..1932
                     /locus_tag="OUD09_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCT9693768.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTSLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTPQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            2041..>2508
                     /locus_tag="OUD09_002546"
     CDS             2041..>2508
                     /locus_tag="OUD09_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCT9693769.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVIDFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQD"
BASE COUNT          779 a          424 c          481 g          824 t
ORIGIN      
        1 tcacttcaat tgtcatgact tatatcgaaa caggtatcaa tcgaaagaaa tctgttatgg
       61 tcatgagtga acattatttg gaagacatcc gccttcgctt aagtgctgag attggacgtg
      121 gccttaccct gctagaggca aaaggcgggt ataatcgaaa gtcaaaagag gttttattga
      181 tcgttgtgac tgatcatgaa ttttcccgaa tgaaaccatt gatagaagaa atcgatccaa
      241 gtgcctttgt cattgtcagc agcgtagcag aagtaatggg acgcggattt acttatcatc
      301 caatcgaatg agttggtaag taaatccgct atttttttga atgcgttttc tttagctggt
      361 ataataaaag aatctagtaa ctgttcgact gaaaaggaga ggagatcttt atgcataaag
      421 gaacggaaat cgattttgta ttaaatgata gtttgaaagc actagaactg tatcaaacaa
      481 tctttgatga aatacaactg attgaggcaa cagattaccc tagaggcaat aatgaagtca
      541 tttttacctt gtatggtacg agattccata tgctggatga aaacacgtct cttggtttga
      601 ctgctcctac tgctgaacat ccaaatacga tctggttcaa tatcatcgtc tctgatatcg
      661 aggacacaca taataaagca ttacgaaatg gctgtgtaga gattcaagaa atgacagaaa
      721 tgatggactt tggtgtgact aactcaatgt tcagagatcc ttttggttat ttatggatgc
      781 ttcatcaggt ccatcgtgaa gtaatttttg aagaaagaaa acaacttttt gagaaatata
      841 tgtaatgttt atgatgagat tattttttgt ctctgatatt ttttgtatat gtttgctatt
      901 cgagaaagcg cgtctgtttc atgtgctttc tcttatttat cttatgccaa tccatttctt
      961 tgctttccac aattatattg tgtaaaattc aattgtataa aatataatta aaggtggtaa
     1021 ttttatgaaa aaaatgtatt ctacgaaaat gatcaacaca ggcggacgct ctggagaagt
     1081 tcataatccc gatcgtacat tcgaattgaa tattgcagct cctggtaaac gcgtcgaaaa
     1141 tgcaaccaac cctgaacaat tgtttgcagc tggtttcagt gcatgtttta acagtgcatt
     1201 agaattgatc aaacagcaaa aaaatatctc tggggcttct actgtcagtg cacaagtttc
     1261 cctttattca gaaagtgaaa cgagctttgt cttaggtgta gagattgaag gatctatcga
     1321 aggtctttct ctggaagaaa cgcaagaatt attagaggca gcgcataaag tgtgtccgta
     1381 ttcaaaagct acagctggaa atatagaagt tactctgaaa gcagttagtg aatagacttt
     1441 tttgtgtaaa tccgctatga tacactatag taaaacagcg gaggtggaaa aatggaagat
     1501 ctatatttag ttaatcaagt atgttttcct ttatatgccg cctctaagga aatcattaga
     1561 cactacactt ccctcctcaa gccgctgaat cttacctata cccagtatct tgtgatgtta
     1621 gttttatggg aaaagaaaaa gacttcagtg aaagagctag gaaatgattt gtgtctggat
     1681 tctggtacgc tgactccctt actaaaaaag ttagcagaaa aaaattatat taatcgtcag
     1741 cgtgaaacgg gtgatgaaag aatcgtaaat gttacgctga ctcctcaagg tgctgcgttg
     1801 caaaaacaag cagaaggtat tccacaaaag atcaagtctt atcttccact tgaacaggaa
     1861 gaattggata cactgcaaca tctgacccga aaaatgctag ataattttca tcaagaccag
     1921 aaaaagggct gaaactgcta gatgaacgat tttatgagga aaaccaactc ataagataac
     1981 gtcatttagc ggtttttctt tttgctcttt ctctttctta ttttcatatt ccatcatata
     2041 atgaataacg tgtttatttt ttgttatttg gaggttgtcg atttgaccgt ttatcagcgt
     2101 tttttggcga atgaaaaaat acgccgtctc actgttttat tatttgtcgt ttttgttctt
     2161 ttcttagtta gaagtatgat tacaaccatt ttgctcacat ttatctttac ttatttgatg
     2221 attcatcttg ttcaaatcat tcagcgattt gtccgaatcc ctacaggtgt tttagctatc
     2281 atgatttata cactgatcgt ttaccttatc tatctggcat tcacaaagta tatcccgatg
     2341 cttattcatc aaacatttga tatgatccag tccgttattg atttttacga gcatcagcca
     2401 aaagatgcag atcctgtttt acaatatatc catacttata ttgagcgaaa cgattttttt
     2461 gaacagcttc aaaatggcgc ttcgattgcg cttggctatt tacaggat
//
LOCUS       DAKBVG010000255         2412 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000255 DAKBVG010000000
VERSION     DAKBVG010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.257"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(<1..1097)
                     /locus_tag="OUD09_002547"
     CDS             complement(<1..1097)
                     /locus_tag="OUD09_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HCT9693770.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVRGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENA"
     gene            complement(1227..>2412)
                     /locus_tag="OUD09_002548"
     CDS             complement(1227..>2412)
                     /locus_tag="OUD09_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT9693771.1"
                     /translation="PGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLVDYDIIE
                     TQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDKNKILMH
                     KDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSAAQELYE
                     NTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWREYNLIKS
                     SSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLLSLGISK
                     LEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQSASSQL
                     GGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNPKELLID
                     NN"
BASE COUNT          731 a          470 c          358 g          853 t
ORIGIN      
        1 gcattctcat tagagaacac tccctttttt gtgacttttc tgaagctaga gtgaatactt
       61 tcaactgcat tagtagtgta catgattttt ctaatggcgc tgccataatc aaagagttgt
      121 tcaacatgag aaaagtttcg tttgcataca tctactgcac cagaataatg agaccaccga
      181 ttttgaaaac tgtcgaaggc agcgtgtgca gcgtttaaag aagaagcact gtagaatttt
      241 ttcatgtctc gacagacttc cttataatcc ttacttggga ggtatcgtaa cgcatttcga
      301 acaagatgaa caatacagcg ctgcacaatg acagaaggaa aaattgcttt tgcgccttct
      361 ttgagaccgg aaacaccgtc catcgaaatg aaaaaaacat cttctacacc acgtactttc
      421 agttcatcaa atacttgcat ccagcgattt ttagattctg tttgattgag ccataggcct
      481 aaaatctcct tgtttccttt aagatcatag ccaagaatgg tgtacacagc atattcttta
      541 gcttcatagt tttcgcgtaa agtgacgtac atacaatcaa cgaatagaaa tgcataacac
      601 ttggctaatg gccgagcttg ccattcttcc aattcaggaa ggacggcatc cgtgatatca
      661 gaaatcattt cgtgggaaat atcaaagccg taaatatctt caacggttgc ggcgatatcg
      721 cattgactca ttcctcgtgc atacatggaa agaaccttac cttcgatgtc ggagacatct
      781 cgttttctat taggaatcaa ctcaggttca aaagaagcct cccggtctct gggaacatca
      841 atgtttactt taccaaagct cgttttaagc gtcttattgc cataaccgtt tcgtcgattg
      901 tcatgtttct ttggctcttt agaatgggcg tcatacccta aatggttatt caattctcct
      961 tgaagcattt tttcaaataa gggaccaaac acatctttta aaacatcttg catatcctct
     1021 acagattcgg gttgataagt attcaaaatg gattcagcta acttctcagc atcaggattt
     1081 cttttctttc tagccatcgt gtaatcacct cttgatctta ttttagaaaa ataaaaaccg
     1141 tgaagcaacc acccgcctag gattgatggt tacttacacg gtttatatta cactctcatt
     1201 tttggtgcta aaaagcaccg aaaattttaa ttattgtcaa ttaataattc ttttggattc
     1261 cttcttaata tattgaatga agaaataatc aatgatacaa gaagtactaa actcataaat
     1321 ataactacat aaatcatgaa tttaggtaat acattgatat ctaaacttga taatgtcttg
     1381 ttaaatcctt cagcttctgc cccaccacct aattggctag atgcagattg tttagctatt
     1441 tgtttagcta tatcaccagt aaccttattc aatatattat tcccaagctt gtcagctgta
     1501 tattgagcta agaaatagga accaacgaat gcggggattg atataaatac catctcaatt
     1561 aggaattgac caaaaatttc taattttgat atacctaatg atagtaatac tgctatttct
     1621 ttctttctag cattcatcca taagaataat aataatgaaa ctacaactcc tgcaaatatt
     1681 aatgagccaa aaaataattt atttgaaatt gagtaaatac ctgatataga ttgttgtaat
     1741 gcagggtaat ttgatgaact tttaatcaaa ttatattctc tccaatttat atctaatttc
     1801 ccaagatctt ttattacact atcaagattc ttatcacctt ttacaaagaa tgttgcatct
     1861 tggtatacag ctgtatcttc ggtattacca taaactttag cagctgtatg aatatcagtg
     1921 attaatgtat tttcgtatag ttcttgtgct gcacttactc caccgctatt atgcccatcg
     1981 aatagacctt taatttctac ttctactgtt tcatctgcac ctttctcatt atcagcatca
     2041 aataaattag attttatttt tatcttatct ccaactttaa ggttattttt ttcagctaaa
     2101 tctttatgca ttaagatttt atttttatct tcattttcta aatgctttcc ttctactaat
     2161 ttatatgctt ctgatacaaa ttttgtttct tttgttgagt cgttaacccc agttaacata
     2221 actgttcttt taaaattctt tgctctttca ggtgattgat tcgcaagagt ctcttgagtt
     2281 tcaataatat cataatcaac taaatctgca acactgttta ttctttttac ataggagtct
     2341 atactatctg tttgagatat ctttttaata tcttcacctt ttacatttcc cccacctcta
     2401 ggtgttcctg ga
//
LOCUS       DAKBVG010000256         2398 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000256 DAKBVG010000000
VERSION     DAKBVG010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.258"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            <1..640
                     /locus_tag="OUD09_002549"
     CDS             <1..640
                     /locus_tag="OUD09_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3-like element IS1476 family transposase"
                     /protein_id="HCT9693772.1"
                     /translation="KVKYLVMDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQL
                     RVQIMNRFRTSHSENQKKYRRLKRYWKLLLKDSTTWEPLKRHYHRLFKRPISQTEIVD
                     ELLSYNEELRTAYHFCQLLRYYFVKRGTEGFQETLKTISSTLPQSFKKKFTIFHRYQS
                     GISNAFKVSHSNGVTEGLNNKIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            695..2260
                     /gene="guaA"
                     /locus_tag="OUD09_002550"
     CDS             695..2260
                     /gene="guaA"
                     /locus_tag="OUD09_002550"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCT9693773.1"
                     /translation="MSSPTRFDEEPKIIVLDYGSQYNQLITRRIREFGVFSELMSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDEKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGK
                     VKPAKNREYGKAELEITSAEAALFQNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCP
                     IASIQNAERKMYGIQFHAEVRHSEYGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGK
                     FGLNIIKVDAKERFLSKLAGVSDPEEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGELTEEKLEIVRESDAILREEIANAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
BASE COUNT          748 a          507 c          496 g          647 t
ORIGIN      
        1 aaaggtcaag tacctagtga tggacatgaa tgccagctat tgtcagttgc ttaaaacagt
       61 ctttccaaat gcggagatcg taactgaccg ctttcatatt gtccaacaca tcaatcgcag
      121 cttcaatcaa ttacgtgtcc agatcatgaa tcgttttcga acctcacact ctgaaaatca
      181 gaagaagtac cgtcggctca agcgatattg gaaactctta ctcaaagatt ccacgacatg
      241 ggagccactc aaacggcatt atcatcgact gtttaaacga ccaattagtc aaacagaaat
      301 cgttgatgag ctgttaagct acaacgaaga attgcgaact gcgtatcatt tttgtcaatt
      361 attacgctat tatttcgtta aacgaggtac ggaaggcttt caagaaaccc ttaaaacaat
      421 ttcatctacg ttacctcagt cgtttaaaaa gaaattcacg atcttccatc gctaccaatc
      481 gggtatttca aatgccttta aggtgtccca ctcaaacggc gtcactgagg ggctgaataa
      541 caagatcaag ctgattaaac gaattgcgtt tggttatcgc aacttttata actttcgggc
      601 acggatctat ttacaacaag gtctgatctt tgaaaactaa aaaaatatgc ggcgcacaga
      661 acgcgccgca tatcggacta agttttattc aaatttgtct tcaccaacac gatttgacga
      721 agagccaaaa attatcgtac ttgattacgg tagtcagtat aaccaactga tcactcgccg
      781 tattcgtgag ttcggtgttt tctcagagtt gatgagccac cgcattaccg cagaagaagt
      841 aaaggcaatg aaccctaaag gaatcatcct ttctggcgga ccaaacagtg tttatgatga
      901 gaaggccttt ggtattgatg aaaagatttt tgatttgggg atcccagttt taggaatctg
      961 ttacggtatg cagttgatta cttataaatt aggcggaaaa gttaaaccag ccaaaaaccg
     1021 tgaatacgga aaagcagaat tggaaatcac ttctgcagaa gcggcccttt tccaaaacac
     1081 tcctgaaaaa caaaccgttt ggatgagtca tggtgaccta gttacccaag caccgactga
     1141 tttcgatgtc gttgctacca gcaaagattg ccccatcgca tcgatccaaa atgcagaacg
     1201 caaaatgtac ggaatccaat tccatgcaga agttcgccat tccgaatatg gaaatgactt
     1261 gttgcgccat tttacactag atgtctgcca atgtactggt gactggacaa tggataactt
     1321 catcgacatg gagatcgcaa aaattcgtga acaagtcggt gacaaaaaag ttttattagg
     1381 cctttcaggc ggagtagatt cctctgttgt tggtgttctt ttacaaaaag cgatcggcga
     1441 tcaattaaca tgtatcttcg ttgatcacgt attgttgcgt aaaggtgaag cagaacaagt
     1501 tatggaaagt cttggcggaa aatttggctt gaacattata aaagtcgatg ccaaagaacg
     1561 tttcttatca aaacttgctg gcgtgtctga tccagaagaa aaacgtaaaa tcattggaaa
     1621 tgaatttgta tatgtcttcg atgatgaagc aaccaaactt gccggtgaag aaggcgtttc
     1681 tttccttgca caaggaaccc tttataccga tgtgatcgaa tcaggaactg aaactgccca
     1741 aaccatcaaa tcacatcaca atgtaggtgg attacctgaa gacatgcaat tcaagctgat
     1801 cgagccattg aacacattgt tcaaagacga agtacgtgaa ttaggtaccc aattaggcat
     1861 gcctgacagc atcgtttggc gccaaccatt ccctggacca ggcttaggta ttcgtgtcct
     1921 tggtgaattg acagaagaaa aattagaaat cgtccgtgaa tcagacgcta tcttgcgcga
     1981 agaaatcgcc aatgccggtt tagatcgcga catctggcaa tacttcaccg tcctaccagg
     2041 cattcgctct gttggggtta tgggagatgg ccgtacgtat gactacactg taggtatccg
     2101 tgctgtcact tctattgacg gcatgaccgc tgactttgcc cgtatccctt gggaagtctt
     2161 gcaaaaaatc agtgtccgta tcgtaaatga agtcgcacac gttaaccgta tcgtgtatga
     2221 tattaccagc aaaccacctg caacagtgga gtgggaataa agagccaaca taatatggat
     2281 gaggaatacc tataaatcaa cattattcaa ttactaaagt tcctgatttt cgtaaaaaat
     2341 aagatggttt cctaccattt tccctaccaa agcccttgtt agattaacta ctaacaag
//
LOCUS       DAKBVG010000257         2390 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000257 DAKBVG010000000
VERSION     DAKBVG010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.259"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(7..252)
                     /locus_tag="OUD09_002551"
     CDS             complement(7..252)
                     /locus_tag="OUD09_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693774.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(269..1120)
                     /locus_tag="OUD09_002552"
     CDS             complement(269..1120)
                     /locus_tag="OUD09_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCT9693775.1"
                     /translation="MNSLTSTYRLLNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQPTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            1286..2290
                     /locus_tag="OUD09_002553"
     CDS             1286..2290
                     /locus_tag="OUD09_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HCT9693776.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT          764 a          404 c          481 g          741 t
ORIGIN      
        1 ttctttctat tttttcggta tcaaggcaaa aacaggtagt tcaggtgaat aaatgacttc
       61 atgttcgtgc cagggtttga ccattagcga atgatcgaac cgttttttca gttcttcgat
      121 gatttgatca tgtgtccagt ttcttgcagg aaaatgttgg attttctctg catcgatcaa
      181 aaagacaaaa tcggaatgaa aagtctgggt aacttcttcc gagagagaat tcaagcgttc
      241 gattggatcc ataaaatcac ctttcgactt aaaattgggt agtatctggg tcttttgctt
      301 tgccgactac accatctaac tgatcgattt gtttcatttc ttcttctgtt aattcaaaat
      361 caaataattc agtattttct ttgattctgc ttggtgtcac tgacttagga agaggaagga
      421 attcgtgttg cagcgaccaa cgcaaagcaa cttgtgcaat cgtcttgtca tgtgcttcag
      481 cgatttgttt catttcaggc acatcgaaga ttttaccagt gcctaatgga ctatatgctt
      541 cgaggatcat atcatgtttt tttgcatact caactacagt tggctgtaat tcgcctggtg
      601 caaggaaaac ttgattgacc atcggcataa tttttgctgt ttttgctagt gtatctagat
      661 ggtggggtaa gaaattgctc acaccgattg ctttgatttt acctgcttca tacagttctt
      721 caaatgctcg ccatgtatct gcatttgctt gttcccaatt atcacgaaaa gcaacagggt
      781 ttggccagtg gatcaaaaac agatctaaat aatctgtttg gagcttcttc aatgattctt
      841 caaaagaaga catcacaagt tcatagctgt ggtttgcatt ccataatttt gttgtcaaga
      901 atatctcttc tctaggaatt ccactttcct taatcgcttg gccgacactt tcttcattct
      961 tgtatccttg ggcagtatcg atatggcgat aacctgcagc cagcgcttct ttaacagagg
     1021 aaacagcgac atccccatca ggtgtttgcc atgtgccgaa tcctactacc gggatttcgt
     1081 aaccatttaa tagacgataa gtgcttgtta atgaattcat actttcagct ccttttttat
     1141 tcgttcaact ataagtgtag cgcatttttc gaaaaaaaga atccattttg tttctactct
     1201 tgaaattttt gtttaaaaga aaatgatttt gattctggta tttttgattt tttttccgta
     1261 aactaagaca gaagggaatg agaaaatgga tatctattta aaaaaagcaa ttctgcatat
     1321 tattgacaga gaaactggtt ctccagtctt ttcacaaaaa gagctggatc tgacaaaaga
     1381 atatattcgt gattttcttc aaaagaagat ccaaaaaatc tcttctgctc aaacaaaaac
     1441 tggacaacta gcagaagaaa gcatgatcgt tgaatcactt aaaatcgctc aagaaaattt
     1501 tgtggaagca agtgaagcaa tcgttcagcg gtggttcgat atctaccaag aaagtgaaga
     1561 agcacctagt gcagatgtat ttgttgtttt gtatgaatta gatacagtga tgtacttatc
     1621 tttattgaaa gtcaattatc gtgaggctta cactcacttt gttgaagcag acgaagcagg
     1681 gatcgacaac cgcttgattt tgaatcgagc aattctaggg gcgaaaacac aaaaagcaga
     1741 tgaagcattc gctgtgaatt tgactgattg gacctacgaa ttaattgaaa aaaaatatga
     1801 gttttctggc aaaaaagagc tttatttttc tggaagagtg atcgaaagtg ttcctgcacc
     1861 ttctttagaa gaaaatgtaa aagtgatcca aaaagtagcc aagcatcttg gaaaaaaatt
     1921 tgagacagaa gaatttgata tcatggctga tttgaaagaa gctgtctatg atacgataga
     1981 agaaaaaggt cggttggatc atgagatgat tgcggaaaaa gtcttcaagg aaaacatcac
     2041 ggcaaaaatg gcttttaaag aagaagtaca agagcaagga tttgtcccag aagcacctcc
     2101 tgtaaaagaa gtacaagaaa tttctgagaa aaaatttgga aaacaaaaat tcaagctatc
     2161 aaacgggatc gaactgatcg taccagtaga tgtatatcgc aacccagatt tgattgaatt
     2221 tgtgaataat ccggatggta cgatatccgt gatgatcaaa aatgtggatg aagtattgaa
     2281 tcgtttataa aaatgactag aacattaggt gttcaagtac cagtgatcag ttaaatgaat
     2341 tgtgtcaaga gaaggatcgg aaaagacgaa tataacaaaa aaagagcgtg
//
LOCUS       DAKBVG010000258         2386 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000258 DAKBVG010000000
VERSION     DAKBVG010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.260"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            233..2377
                     /locus_tag="OUD09_002554"
     CDS             233..2377
                     /locus_tag="OUD09_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCT9693777.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQTADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFEPVPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
BASE COUNT          833 a          501 c          450 g          602 t
ORIGIN      
        1 ccatttttat ccccacttgt ttatccccaa acaaaggtgg tctatgcaat aaaatgaacg
       61 tcaatgaaaa acgaatgaat gctcgttgct tatttacaaa ttgttttatt tagaatacac
      121 agacagattt atgaaaagaa aaagcttttc gacacgatta tctgttccaa aaaatgtcta
      181 atgaatgctg aataagacag aaaatcaatg aaaggaaaag gactagttta ttatgaaaaa
      241 taaaaaaacg atacgtttgc tagctgctac ttggctgatt gctccgtctg ttttgtcgac
      301 caataccgta tttgccgtag ataatcaaac agctgatcaa acagtcacaa cagatacgac
      361 tacatccagc tcagctacta gtgaaacaac ggattctttt acagaagacc caactggaac
      421 aactaactca acaacaggat cgaccgagcc aactgaaatt gatccggctg atccatcaga
      481 aaatgaaaag ccagccgaga catattccct tacaattgct ccttctttga ttggtaagat
      541 ccaactgact gcttcgacag cggattcccc tgaacagcaa cctttggata ttacggaaag
      601 tggaacggta gaaggtttat tagcggaaac gaaggtaact tacactatta cgccagctga
      661 aggtgaacaa attgcttcag tcgctgttaa tggtgccaaa gaagtaaatt atgatgcaaa
      721 gacctttaca gtaggtacga acaatttatc tattactgca gaatttgaac ctgtaccgga
      781 tacttcaaca gagaaaccaa ctgatcctag tgaagaagtc cctagtacac cagatccgga
      841 aacgccaaac aaaccagatg tgggtaataa caacaataat aatggttcaa ataattcaaa
      901 caataataac aataattcga acagcgggaa ttcttcgaat caaacaaacc catctggatc
      961 gaataacaat ggaaatactc agagagaacc aaaccatcca aacaatggtt catcaaatgg
     1021 cggaacgaac aatgcttcat ctcgtccaaa tacagcaaat caaagcggta tagagaatcc
     1081 atcttctgct tcttctgatt ttgttgtaaa atcaccaatt gatgctgtat tgcctacaaa
     1141 tacaacgaat gtccaacaag caatcgttcg agaagcattc aagcatcttg gaaaaccata
     1201 tgtatgggga gcaaaaggtc caaatacttt tgactgttct ggtttgactt attacgtata
     1261 tatgaaagca accggtcatt atatcggtgg ctggacaggc gaacagcaat atgcaggtac
     1321 acagattcca gtaagtcagg cccaacctgg tgatctcgtc ttctggggac cttcttctgg
     1381 tgttacccac cacgtaggta tctatattgg gaacggacaa tttatccatg cgcctcaacc
     1441 aggagataaa gttcgagtaa cttctatttc agatttcacg cctgattttg ctgtccgagt
     1501 aaacttagca ggtttacctg ctgcttcagg ttcgctgaca ggcggctcat ctattttaga
     1561 tggattagat ggctcgttcc atttcagtca aaaccaaaca actgatcagt tcttgaaaaa
     1621 gattgcagat gatgcacagg aaatcgggca aaaagaagga atctacgcat cagtcatgat
     1681 ggctcaagct atccttgaaa gtggatctgg aaacagctta ttatcaagcg aaccaaatca
     1741 taaccttttt ggtatcaaag gatcatataa aggaagcagc gtgaccttca atacacttga
     1801 acaagatagt tccggacaaa gctaccaaat ccgcgcacaa ttccgtaaat atccttctta
     1861 caaggaaagt ttggaagact atgccgactt gatcaaaaat ggactgactg gcaatcctga
     1921 tttctacaaa ccaacttgga agtcggaaac aaaagactac aaagaagcaa ccaaatattt
     1981 ggaaggacgt tatgctacag atcgccaata ttcacaaaaa ttgaacgcga tcatcgaagc
     2041 gtacgacctg acaaaatatg atgaaccgaa aaaagaagca gcaacaactg aagaagcaaa
     2101 cgctgaaatc ctcaatatgt ctaagcgatt cgatgtgcca atcaaatgga caacagatgg
     2161 cgtgccaatc acaggattag aattttcaac gataaaacgc gtcaacaacg cttcttccat
     2221 cttccgtgtc gcttcaatct gggatttgtg gaacaatttt acggcaagac agataccaga
     2281 aacaactact cgtacagtta aagctgcaac tgttcctgta ctaaccttgt taagtttaga
     2341 caaatcattc tggcttgatc tgcctttaaa gaaatagaat aaatta
//
LOCUS       DAKBVG010000259         2350 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000259 DAKBVG010000000
VERSION     DAKBVG010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.261"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            31..918
                     /locus_tag="OUD09_002555"
     CDS             31..918
                     /locus_tag="OUD09_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCT9693778.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            1004..1234
                     /locus_tag="OUD09_002556"
     CDS             1004..1234
                     /locus_tag="OUD09_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HCT9693779.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(1291..2256)
                     /locus_tag="OUD09_002557"
     CDS             complement(1291..2256)
                     /locus_tag="OUD09_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HCT9693780.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRAQLPDLQ
                     EMTFEQKKKELQGLFQGQNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKHFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMIASADVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQSEIELLNKKEAEKQLN"
BASE COUNT          782 a          385 c          474 g          709 t
ORIGIN      
        1 aggatttttg atttttagga ggtcgaaaaa atgaaacttg gttttatcgg aacaggcgtc
       61 atgggaagtg ctgtcgcacg ccatctacta gaagcaggac acgaagtagc tgtctataat
      121 cggacaaaag caaaagctga tcctttagta acagaaggag ctatctgggc agatacacca
      181 aaagcggtag cagaacaaag taatattctc tttacaatgg taggttaccc caaagatgta
      241 gaagaaatct actatggaca gtcggggatt ttttctgcag atatctcagg acatatttta
      301 gttgacttga ctacaagtac accaagcctg gcagaaaaaa ttgcaaaaac agcaaaagaa
      361 aaaggcgcag atgcgttaga tgcaccggta tccggtggtg acttaggagc aaagaatggt
      421 acactgacga tcatggtcgg tggagaagaa gccgtttatg atcaagtact gcctttattc
      481 aaagaatttg gtacgacatt tacattgcat ggttctgctg ggaaaggtca gcatacaaaa
      541 atggctaatc agatcatgat tgcaggtacg atgacaggaa tgacggaaat gcttgtctat
      601 gcacaaaaaa acggacttga tttaaagaaa gtgattgaga cgctttcagg tggaagtgca
      661 gctaactggt ctttaagcaa ttattctcct cgtattttaa aagaagatta cacacctggt
      721 ttttttgtca aacattttat caaagattta aagattgcat tagaagaagc agaaaaaatg
      781 gaccttgtat tgccagcaac tacccaagca ttgaaactat atgaagagtt ggcagataaa
      841 ggttttgaga atgacggaac ccaagctttg atcaaacttt ggtggcctga aggaaaaata
      901 ccagaaaaaa aatcataaaa aacgagtttt tttagagaaa atcaatgcaa gtaggctatg
      961 cttttgatac aatataacag aaataagaaa aggaggacct ccgatgacac attcacaatt
     1021 agtcgcaatc ataaaacgac tggaggctat ggtcgagtca gcagataatg aactgcaagt
     1081 tcgccgtttt gaaaaagaag gcgtagagaa gtgtacagta acttatgata aagcgaccga
     1141 tacatttgaa ttaactgaaa cagatactca tcagcaatac gaatttgata atatcgatat
     1201 cgtagcgatg gaaatctatg acttaatcca ataaatttta ggataattga catttgaagt
     1261 tgaagatgcc taaaagaagc cgagaagcaa ctaatttagt tgcttttcag cttctttttt
     1321 gtttaacagt tcaatttcag actgttcttt ttctaatttt ttgcggtaac tgatcaagat
     1381 ttgtacagga tcgatttctt cttttgacaa ttgacgtttc aaagataaat gagcttcttt
     1441 cttttcataa tagaagtcag gagaaacgtc agcagaggca atcattgttt ctgcaagttg
     1501 accataacaa acgaggtagt gctttttctg actctcatct gctgctaatg gcatgctgaa
     1561 ggtgagcgat ggtgttctta aaaaatgctt ttgttcctgg atccgtctaa taaaataagt
     1621 aactaattcg atatcttttt gaagcatacc ggctttttcg aaattcaact gatagatgaa
     1681 atgttgttgt ctttttttca agtaagttag tagcttttta ttttggcctt gaaacagccc
     1741 ttggagttcc ttttttttct gttcaaaagt catttcctgt aggtcaggaa gctgggctcg
     1801 caaagctaac aaactaattt cagacaacca aggcatttga taagtttctt ccattatacg
     1861 caaaatagac ggcatttttt tatagagacg aaaaggcccg tatgttcgag ccgtaggagt
     1921 atctgttaat acaaatccct tattggtaat atgtacataa ttataattac tgaaataatt
     1981 catttgtcgg ttataaaaag gacggtaatg ttggatcaat tgacactcga gcaataaagc
     2041 atctaattcc gtgtctacta cgacgaaatc aaaatctgtt atatgatgaa tcatacgtaa
     2101 tacttttttt gaatgctgtt tattctgatg aaaatagctg cttactctat ttttgagccg
     2161 ttttgcttta ccaacataga tgaccttacc atcacggtct ttcataagat agacacccgg
     2221 cagcatgggg agagttttca ttttttcttt taacatagga tctcctttta cgctttgaat
     2281 aaaaagacta taacaaattt tgaaataatg gacaagtttt aaggaattct atagttctgt
     2341 tcacagaaaa
//
LOCUS       DAKBVG010000260         2303 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000260 DAKBVG010000000
VERSION     DAKBVG010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.262"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            292..909
                     /locus_tag="OUD09_002558"
     CDS             292..909
                     /locus_tag="OUD09_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCT9693781.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            950..1249
                     /locus_tag="OUD09_002559"
     CDS             950..1249
                     /locus_tag="OUD09_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HCT9693782.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            1283..1450
                     /locus_tag="OUD09_002560"
     CDS             1283..1450
                     /locus_tag="OUD09_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCT9693783.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(1495..2100)
                     /locus_tag="OUD09_002561"
     CDS             complement(1495..2100)
                     /locus_tag="OUD09_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCT9693784.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT          773 a          369 c          428 g          733 t
ORIGIN      
        1 caaattctga tgtgaaaaat aaaatatacc atggaagaca taaaagaaaa actggataac
       61 tagtttattt aatggcagac cgttaaggcc tgccattttt atgtatttta gcggaataaa
      121 tataatcatg cgggagtgta aacttactgt ttcaaaaagt atactttttg acatgccttg
      181 attttctttg tttatgtatg gcataataac cacataatac aatttgaaac gtttctgatt
      241 taatctgatt gttttgaaac aattttagtg aaaaaagggt agataaaaac gatgagaaaa
      301 tttttttata tccgggtcag tagttatgat caatcgatcg aacgacaact gatttccgcg
      361 aaggaattag gaatccctga agaatatata tttgtagaaa aagccagcgg caaagatttt
      421 aaacgccctg aatatcaatt aatgaaacgg atgtttcgaa aaggagattt actctatcta
      481 caatcattag atagactagg cagaaataaa caaatgattt tagaagaatg gcgagaatta
      541 actaaggaaa agcagattga tatttctgtg ttagacatgc ctctgttaga tactgctagt
      601 tacaagcaac tagatgggtt agagacgctt atctctgact taattctgca attactcagc
      661 tatatggccg aagacgagcg gaaacgaatt aatagccggc aaagagaagg cgtagaagct
      721 gcgaaacaaa agggtgtaaa attcggcaga aagaaaattg ctttacctcc tgaatttcct
      781 gcgatctatt ctgactggaa agcaggaaag ataactgcag tctatgctat ggatcaattg
      841 ggcatgaaac gaaatacctt ttatcgacgg gtgaaagaat atgaacatac cttgcaccac
      901 ttttcttgat agatataaca gctattagac gggaagggag agtttgaaaa tggacttaac
      961 gaatcaaagg gtcgttttta ctggaacgct gcagcaaatg acacgaaccc aagccatcgg
     1021 tttggttcac tctttaaacg gccattgtca atcaagtgtt acttcaaaaa caaatttcct
     1081 ggtttgtggt cagtattcta agtcgttgtt tgatgattct ctctatacaa aaaaggaaca
     1141 aaccgcgcgg gacttgattg cgttaggtca tgagattacc attttatcag aagtcgaatt
     1201 ttatgcgtta attagtcaac agttgaaaga atggcaacgg tatttataat cgttagtaaa
     1261 atttacaagg aggaaaattt ttatgaagcg aaaatcttat gatcgttggg gcattgaaat
     1321 aactagtttt aatcgaaata ttgcttttca cgtggagtgt gactgtgggg aattagcaaa
     1381 aagtgtttat caaaaaaatt acttttactg tcctaaatgc catcgccgat acggggtaca
     1441 tggaacatag aaacgaaata gtgcggtaga aaagcgaatg aatgtagtat atttctacaa
     1501 atcattcgct ttctttattt tataaatagt tggtcgagag atcccgtttt cttttgcaat
     1561 ctctgcaact ggaatgcctt gctttaacat gtccaccacg gttttaaaaa ctaagcgttt
     1621 ttgcggatct gttgagttaa tagaatactc gattggtcgt cccttatatc ggccggcttt
     1681 ttgagccaat aatactcctt gccgctgacg ttcacgaatt ttctcacgct cgttttgtgc
     1741 aatataactt agcagtgata aaaacatatc gaacatagcg gtatccagga gttcatttcc
     1801 agtattaaaa tctaaaaact gtgcatctaa tatttttaac gctacttttt tctgtttcat
     1861 aaaagcaact gtatttttta tatcttcata atctcttcct aaacgatcta gtgatgtaac
     1921 gatgacgcaa tcaccttcac ggatataatt tagtaatttt tgaaactcag gacgctcagt
     1981 atctttacca ctggcttttt cttggaataa tttatcaatg ttaagtcctt ccatcattgc
     2041 caattgtcga tccaaatttt gatcttgtga agatactcga atatagccaa tttttcccat
     2101 aaaaaactcc tttttaaaag tgatataatt tacatgtaaa atttacttgt agttttactg
     2161 ctatcatagc atttttaaaa ataaatttta tgtagactta aaatttactt catggagggt
     2221 ttaaatggat gaaatcaaaa gtaacaaaaa agcatttggc atttatttag gagaacctag
     2281 tacagaacaa ttaaaccaat ttt
//
LOCUS       DAKBVG010000261         2270 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000261 DAKBVG010000000
VERSION     DAKBVG010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.263"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(62..508)
                     /locus_tag="OUD09_002562"
     CDS             complement(62..508)
                     /locus_tag="OUD09_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HCT9693785.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(535..711)
                     /locus_tag="OUD09_002563"
     CDS             complement(535..711)
                     /locus_tag="OUD09_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCT9693786.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(873..1301)
                     /locus_tag="OUD09_002564"
     CDS             complement(873..1301)
                     /locus_tag="OUD09_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCT9693787.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            1393..2253
                     /locus_tag="OUD09_002565"
     CDS             1393..2253
                     /locus_tag="OUD09_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HCT9693788.1"
                     /translation="MSSDSTEVVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLKQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
BASE COUNT          670 a          455 c          369 g          776 t
ORIGIN      
        1 ttcttttttt acgaacacaa aaaactgcgg acaatcactg tccgcagttt aaatgataaa
       61 attaagattg taatagttct ttcacaatcg cattcacttg gtttccatct gcttttcctt
      121 tgactttagg cataattgca cccataactt tgccaaattc tttcattgat gaagcaccag
      181 tttgagtgat tgcttcttga acaagttgtc gaatttcttc atcggaaagt tgttccggca
      241 tatatttttc gactattaca atttcacttt ttactttttc agctaaatcg tcacgtcctg
      301 cttcttcaaa ctcatgcaaa gaatcacgtc gctgtttcat ttcgcgagac aacaccgtta
      361 gttcttcttc ttctgtcaag tcgtgacctt ctttaatctg ctcgttttgt atagatgact
      421 tgatcattcg gatgacttgt aaagattcct tatctttcgc tttcatcgct gctttcatgt
      481 catcgtttaa tctacttagt agtgacacac taccaactcc aattcatcac agaactagaa
      541 ttttttgcgt tttctagctg cttcagattt tttcttgcgt cttacacttg gtttttcgta
      601 gaattcacgt ttacgagatt cttgtaaagt acccgctttt gacacggaac gtttgaagcg
      661 acgaagagca tcgtcaagtg attcgttttt gcgaacgact gtttttgaca tgttaattcc
      721 ctccctccga gcttaaaaag taaccgtaat tgttattgaa atatatctca aaaaacaaga
      781 ctgtcctgta aatattatat ctaatgctgt tttgaccgtc aaggaaatct tgcttttttt
      841 taaaattatc aaatgttatt ttatttatac ccttattgac aattttcaca tcttccgaaa
      901 atctcgaatc tatgcccttc gatcgtgcat ccacctagct gttcttcgaa ataattcatc
      961 ggacacatat ggatttcttt cgttgttccg cacactgtac agataaaatg atggtgatgg
     1021 ccgatttccc cctgacagca atgaaaacga aacttcattt ctccatttaa atcggtttct
     1081 tccagtaaat cgatctctgc aaaatcatgg agattccgat aaatcgtatc ataacttacc
     1141 cctttgaatt cctgattcat atagtcaaaa acttctttag cagatacgta gcggttacgc
     1201 ttcactaaat aagttaacaa tgcttctctt tttttagtat atttaaaacc gttatttttc
     1261 aatgtttcta atgattgttc gatcaatgtc tgttttttca tgacttattc ctccaaatca
     1321 aaatgattac gaagtacgca ttctagagta tggtataata aacataaaaa aaatgcaagt
     1381 gaggttttat ccatgtcttc cgattctaca gaagtcgtta ctttttttgt catctccgac
     1441 tctgctggag aaactgcgac aaaactagcg caagctacta tggctcagta tcctaccgtt
     1501 gagttcaacc tattccgcag aacgttcgtg acagatgaaa aaacattaaa gcaggcattg
     1561 caagatgcat tagaagaaaa agcacttgtc ctgcatacat taatcaatca agaactgatc
     1621 gagataacac gttctttttg tgaagaacat caattattta gttttgatgt gatgacgcca
     1681 ccgatatcag aaattgaacg tttaaccggt atcaagccaa ttcggcaacc tggtgccctc
     1741 catttattga atgagaacta tttcaaacgg atcaaagcaa tggaatttgc tgtaaaatac
     1801 gatgatggga aagatcctcg tggtttttta gaagcagatg tcgttttgct aggcgtatcc
     1861 cgaacttcca aaacgccttt gagcttgttt ttagctaaca aaaatttaaa agttgccaat
     1921 ttacctttga tcccacaagc acatattccc aaacagcttt gggagatcga tcctaaaaaa
     1981 atcgtgggac taacgaataa cccagatatt ttaaataata tccgtaaaga acggatgcgc
     2041 tcttacggat taaaccctga tactgcttat tccgatatcg aaaaaattag agctgaactc
     2101 gattttgcga atgatcttta tgaaaaacta ggatgtgtag tgatcaatgt ggcatcttta
     2161 tctattgaag aaacggcttc gcttattttg aatgctttag acctagaaga tcatagttat
     2221 tatggaactg aaacaacaga aaaaagcgat taatcatttt tgtctttttc
//
LOCUS       DAKBVG010000262         2249 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000262 DAKBVG010000000
VERSION     DAKBVG010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.264"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            <1..445
                     /locus_tag="OUD09_002566"
     CDS             <1..445
                     /locus_tag="OUD09_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693789.1"
                     /translation="TILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNKKMS
                     LTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSELVSY
                     KQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            459..1148
                     /locus_tag="OUD09_002567"
     CDS             459..1148
                     /locus_tag="OUD09_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693790.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1273..>2249
                     /locus_tag="OUD09_002568"
     CDS             1273..>2249
                     /locus_tag="OUD09_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HCT9693791.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGK"
BASE COUNT          775 a          300 c          480 g          694 t
ORIGIN      
        1 aacgatacta agtatttttt cagtatttac aatattaatt gggggctatt ttcttttgaa
       61 tttagattct gttaattcaa acgctcaaat aaaagatcaa agagtagctg aacataataa
      121 taaaaaaatg agtttgacca aaaatatcta ttctatgtca gatgaagaat tgtataaaga
      181 atttggtgat ttatccgggg aacagttaga agaaagtatt ccaggaatta gtgactcaga
      241 taaaattgtt gtcttgtctg ctggtgggca attaatggga gatggcaaat ccgaattagt
      301 tagttataaa caaaatcaca gacagtttat tgatgttaat actgattcca acgctatttc
      361 gattaaggat ctaaaagagc ttttaaatag taaaagaaat gaattaaagc agataaaaga
      421 aactaattca gtggaattag agtaaaggaa gattcattat gaaaaagatt tttggtgttt
      481 ctgttgggct acttatgatg ttaacatttg gaaatgcagt atatgctgct gagaaagaag
      541 ttatttcaga tccagagcag tacgcctcac gatactttgc tgattttgaa gaagaagata
      601 aagtacttgc attgccagaa ggtggttttt taaaagggga agctaaaatt gtatctatgg
      661 ataatccaga tataattcaa ggttcatata attcagaaac tgatcctgct tcagtttcag
      721 tgacagaagc aaaaaaaatt gttgctgaaa aattcaaaaa tgatattgaa actactccaa
      781 ttatgcctcg aggagcattt ccacccgatg ttggtactag cggacacctt cttgtactag
      841 caggaggagg aagctataaa tcacagccat ttagtggtgc gggatggcgt tttggaggta
      901 tgggtgtaaa accagctgat ggtacaggca actttttacg ttggaaatcc tacattgacg
      961 atggaagagt aggaaatgta aatcaagcca acgcaactaa atctggttct attcaaggaa
     1021 tggcattgta tgctcgtgaa ggattcaaat actttgcacc aaacgggcct ggttggacaa
     1081 tgaatatagg tatttattac acttataatc ctgttgcagg tacgtattat gttgttcaaa
     1141 atatttaata aaacagcaca aagagatttc tttgtgctgt tttattattt ttagttgttg
     1201 acaaacacta aaatataaaa tataatcaac acataaacga acgtatgttc ggcaagaaag
     1261 caggggattt ttatggatga gatggtttta agtactcaga aatggctaaa caagaaatac
     1321 agtaatgtta cagggtttga taaggttcca gaaaatgggc gaacagggtg gcctacaatc
     1381 tatggactta ttgaaggact acaagtagaa cttggtatta ctaatctagt agctaatttt
     1441 ggacctacca ctgaaaaaat gtacgataat caggttactc ctaaatgggg caaaaatcta
     1501 cctaaaaata ttgtatttct tattcaaggt gccttttggt gtaaggggat taatccaggt
     1561 ggcttcgatg gggtatatac tccatattta gatactgctg taaaagagtt gcagactgat
     1621 gcagggttta atggaagcgc tgtaactgga gttttggatg cgaaatgggc caaagcgtta
     1681 tttgatatga gtgcttttgt tcttgttccc ggcggagata gtaaaattag aaaaatgcag
     1741 caatggttaa atgtaaatta tttagaatac acgggaataa tgccttgtga tggtatttat
     1801 caaagaaatt caaatcaagc attaattttt gcattacaag ctgaactagg atattctccc
     1861 agtgaagcaa cagggtctta tggtaatggg acaactagta aaacttatcc tgtgtctgaa
     1921 ggtaactcgg gaaactatgt aagaataatt caatatggat tatatgttaa tggttatttt
     1981 gaagacggta cattcgatgg tattttcact aaatatatgg gtttagaagt actggcattc
     2041 cgaaaattta tggtattgcc tgaatatact gaaatagcag atgttgttgt aataaaagga
     2101 ctattgtcta gcgctggaga tattagaaga tctgcagatg gcgcagatac ttctacgcaa
     2161 ttaactagat ctcaaatcca aacattggtt gataatgata taaaaatagt tggtagatat
     2221 cttactggta ctgttggaat tggaaaaga
//
LOCUS       DAKBVG010000263         2248 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000263 DAKBVG010000000
VERSION     DAKBVG010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.265"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            82..1695
                     /locus_tag="OUD09_002569"
     CDS             82..1695
                     /locus_tag="OUD09_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCT9693792.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT          704 a          400 c          443 g          701 t
ORIGIN      
        1 taaacaataa ttttccggca caattatttg ctgtttttta ccataacata gtacgatcaa
       61 cgtagaaaag gagatgtttt tatgaaagtt gttgtcattg gcggtgtagc tggcggtcct
      121 tcatttgcca ctcgtttccg tcgattgaat gaagcgcacg aaatcattat ctatgaacgc
      181 ggagaaaata tttcttatgc aagttgtgct ttgccttatt atttaggagg tgtgatcact
      241 gaccgtgact cgctgatcga acgtacacca gaaatattga aaacaaaaaa caacatcgat
      301 gtctttacta aacacgaagt aacagcaatc gatccttcta ctaagcgatt aacagttaaa
      361 gacctatcca caaatgaaga aataaaaaca gattacgata agttgatcat ctcttctggt
      421 gctagaccag attatccgga tattcccgga gtttttgaag cagaaaacgg ctttgtcctc
      481 cgtagtgtga cggatgcgga tcgaatcaaa tcgttccttg aagaaaaaaa tccacaacat
      541 gtcgtcattc ttggtgcagg tgttatgggt ctggaattag ctgagaattt caagcatcgc
      601 ggcttagacg tgactttaat cgatcaattg ccacaagtcg ctttccctta tgatccagaa
      661 attgctaatt tagtttatga caaattgctg aaagaaggat tagccgttca tttagaaaca
      721 agagttactg agatccgtga taaaggtcga gaaatagtat tatcagatgg ctccgtcctt
      781 tctgctgata tgctaatttt tgctgttggt gtttctccga agaatgaagt ggtgaaagca
      841 accggcatac aattatcaga tacaggtcag atcattgttg atgaccagtt acaaaccaat
      901 ctgccggaca tctatgcgat tggcgatatt atcgaaacga ctagtgtagt gactggtcag
      961 ccgatccaaa gtatgctttc cagtgcggcc aatcgtcaag gacatatgct ggcagatatt
     1021 ttaaatggta cgcctatgcg ctatcgcgga tatattggtg caggtgtcgc aaaaatcttt
     1081 gatcatacag caagttatgc gggaatgaca gaacatgaat taaaagcatc aggcataaca
     1141 aattataaaa ctgtttttat cactcccttt gaccatgcct atttctatcc agaagctaca
     1201 agattaaatc taaagctgat ttttgatgca gatagcggtc gtattttagg tggacaagca
     1261 tttggagaaa aaggtgtcga taaacggatg ggagaacttt ctgtagcaat caccggaaac
     1321 ttgacagtct ttgatttgcc tgatttggag ttgccttact ctccaccgta ttctactacc
     1381 cgtgacccgt tgaatatagc tggttatgtc gcaatcaatc aaatgacgaa tatcgtagaa
     1441 acgatcaaag caagtgatat acccgaaaac gatttgaaag aagcgttctt tttagatata
     1501 cgtgaaccta ataaagcacc ttctggcagt atctcggcaa caaaaaatat tccaatgaat
     1561 gagttacgag atcgaatcaa tgaaatccca aaagataaaa aagtttacat tactttcaga
     1621 agaggattga atacttatac ttctgcccga atcttggcag gtttgggtat caaagcgatt
     1681 ttgattgaag aataaaaaaa cgaccgcggt gggtagccgc ggtctaggag ttatttttac
     1741 ttgaggagta aaaatgaaaa agtatatagg gttgttggtt ggtatgtata tataatagaa
     1801 gataaatgtg aagaaattgt gcattatttc ttcacattta ttaggttata gtttaagaaa
     1861 gtttaaacat tgcgcaattt aattatttta gctgtttctt ttcataaaaa aacatgttgt
     1921 gactctgaga caaatcatag tccatagtga tgtctctaga gtaactacgc ctaatattac
     1981 gaggcatcat attaatctat cagcactagt acctaaacta gcatcccttt cgaatatttt
     2041 ttcttatata ataaagtttg ctttcactcc tctaatctca ccaaatcgac tggcttgcgt
     2101 tgtgtgatac taggaactga ttggtttaga acgacttttc tattgtaatc atatttttcg
     2161 ccatcctcgt tgtcttctaa tccttcaatg aatgatccct aatgtgtgta ttaaataatt
     2221 taattttttt gttcctattt atttaaca
//
LOCUS       DAKBVG010000264         2236 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000264 DAKBVG010000000
VERSION     DAKBVG010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.266"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(273..1154)
                     /gene="galU"
                     /locus_tag="OUD09_002570"
     CDS             complement(273..1154)
                     /gene="galU"
                     /locus_tag="OUD09_002570"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCT9693793.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(1197..2216)
                     /locus_tag="OUD09_002571"
     CDS             complement(1197..2216)
                     /locus_tag="OUD09_002571"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCT9693794.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT          582 a          514 c          355 g          785 t
ORIGIN      
        1 aaaaaattcg cctttttatt ctactatgaa tcttctggct tggctttcat ttctcttatg
       61 taatcatttt cttttataca acttaaaaaa aaattaaacc attagcataa tattttctga
      121 atctattcac gaaagtcttt ttctatcttt cgttttccac gactcaactg atctagagtc
      181 aaaaaactaa aacgacagtt gcgcaaatga cgcacaagct gccgctttaa taacaaatag
      241 cctttcattc tcagcaggcc acttatttcc agttaacttt ctgttcttag ctttttactc
      301 aactggataa tatatgcact taatgaagtc ccaatttccg aatgttgaag tccatattca
      361 atatttgtct tcatatatcc taacttatcg cctacgtcgt atctttttcc tttaaactct
      421 ctcgcataga ccgcctctgt ctggttaaga gtctgaagtg catccgttag ctgtatctcg
      481 tttcctgctc ctggttcttg agtttctaat atctcaaata ttttaggagt cagtagataa
      541 cgtccgataa tggctaaatt acttggtgct tttcccattt caggtttttc tacaaaccct
      601 tttactctgc aacgaccctt ttcgctatct gttttaggat caataattcc gtatttagat
      661 gtttcatttt ctggtacacg catgacggca acagcggaag cttgtgtttc ttcataatcc
      721 atcatcaatt gttgagtcaa tgggatatca tctttcatca aatcatcccc cagcatgacg
      781 acaaacggtt catttcctat aaatgctttt gcttgaagca cggcatcccc cagtcctctt
      841 ggatgagatt gacggatgaa gtgaagattg acttgagtcg tttcttcaac taaagcgagt
      901 aattctgttt tttccttttc ccgcaaattg ttttccaatt cgatatttgc atcaaagtga
      961 tcttcaatgg gtcttttttc ttttcccgtt acgatcaaga tgtcttcgat tcccgaatcc
     1021 aatgcttctt ccacaataaa ctggatcgtt ggtttatcca cgattggaag catttctttt
     1081 gccatggctt ttgttgccgg caaaaaacgt gttcctagcc cagcggcagg aatgactgct
     1141 tttttgacct tcacttgctg ttcctcctaa acgacgatta actattgatc atctatttaa
     1201 cgaagagaaa attcattctc catttttccg tcacgtaaca tgatttcttt tgctgctttt
     1261 ttgatatctt ctccattata caaaacatta tagattgttt gagtaattgg catctcaaca
     1321 cctagctgtt gagctagctc tactgctgct tttgtcgttg ccacaccctc aacgaccatt
     1381 cccatgtttt ctagaacttc ttccagctta tgtccctgtc ctaacaagtt accggcacgc
     1441 caattgcgag aatgaacact tgtacaagta acgatcaagt cccccacacc actcaaaccg
     1501 ataaatgtca gaggattggc acccatcgcg acaccaagac ggctgatttc tgccaatcct
     1561 cgagtcatga ttgctgcttt tgcatcatct ccgaatccta atccatggat agcgccggca
     1621 ccaattgcaa tgatattttt cagtgctgca ccagtttcta cgccaatcac atcattattt
     1681 gtataaatcc tgaaataatc attcatgaac agtttttgta cataggctgc tgctttttga
     1741 tcaatacttg cagctgtaat tgttgtgata tcatgtactg ccacttcctc agcatggcta
     1801 ggaccagata atacaacaat tgcctgtctt ttttcttctg gaatttcttc agccaaaact
     1861 tctgagattc gtttatgcgt atcttgttct agtcctttac ttgcatgtat aatgattggc
     1921 ttagtattca ttttaccaat aagttcctgt gcgactgaac gaatcgcttt tgttggaaca
     1981 acaaataaaa tagcatctgc gttatccact gcttctgcca attctttata tccgataatc
     2041 gattctggga tcgtcaaatc aggtaaaaaa tgctgatttg tatgatactt attgatttca
     2101 tcaatctgtt caggtacatt tccccaaata cggacatcat gcccgttttc tgccaatgtt
     2161 tgtgctaaag cagtgcccca agatccagga ccaagaactg cgattttttg tttcatggat
     2221 attcctccaa ataaat
//
LOCUS       DAKBVG010000265         2223 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000265 DAKBVG010000000
VERSION     DAKBVG010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.267"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            383..838
                     /locus_tag="OUD09_002572"
     CDS             383..838
                     /locus_tag="OUD09_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693795.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQQAAAEQASQQAQAAAP
                     EQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSKCYGN"
     gene            complement(1000..2172)
                     /locus_tag="OUD09_002573"
     CDS             complement(1000..2172)
                     /locus_tag="OUD09_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCT9693796.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
BASE COUNT          717 a          443 c          398 g          665 t
ORIGIN      
        1 atacttaata acagaactat agcaaaaaat cccgtgtatc aaaacgggat tagattaagt
       61 ttcctcaatc actattcata acacaattat tataacaagt aagtaaacaa ttgaaaagga
      121 catttattga aaacggttgt tttttactta aggaggaaat ttagtgaaaa aattagttgc
      181 attgtttttg gtcgcaggaa gtttgttgag tgggtgtggt gctactacgt ctactactca
      241 aagcacaagt tcagctcagg cttccagcag cagtaccacg atctccgaaa gtacggcaga
      301 ttcatcgaaa gaacaagaac aaattgaaaa gaaaaaagaa gaagagaaac aaaaagcgga
      361 aaaagctgca ctagaaaaaa aagtgacaga agcggaatct gctttgtcac aagctgaagc
      421 aaatcctaca aaagaaaact atgcaactgc ttcagcagct atccaagcgc tacctgaaaa
      481 caaacaagag ttgacaagcc gtcttgctgc aattgattcc acaatcaaag caaaagaagc
      541 agaagaagaa cggaaaaagc aagaagaaat tgcacgacaa caagcagaac aagcgcaaca
      601 agaagcaatc gcacagcaac aagcagaaca ggcgcgacag caagcagcag ctgaacaggc
      661 ctctcagcag gcacaagccg cagctcctga gcaaaataat gaacaaactg tctatgtcac
      721 accgaccggt tcaaaatacc atacacataa atgtggaaat ggtacgtata cccctgccac
      781 tttaagcgaa gctcaagctc gtggtttaac cccttgctcc aaatgttatg gaaactaatt
      841 caaaagttat actataataa taaaattctt ctgaaacata aaaaaataga agctggcgtt
      901 ccagatagta aaccaaaaat agcagtttac acgtccattt cgtcagcttc tctctttttt
      961 catttagtct gttccctctg cttttttgga catcttcatt cattctttct caatcaaata
     1021 gtacacctct ttttcttttc gcagtttata tcttcctttt ttcaatttca ttgaaaactg
     1081 tttcgaatgt ttatgtttta catggatttt aagcaggagc tcctcatgtt gcaagcgata
     1141 gttcctgcct tttaaatcag aaaatctcac tagtccgtat tcatcagaag ctttcacaaa
     1201 tggaactttt tctctagtaa tatagtgttt gccgttgagc gtttttacag aaaagaccat
     1261 tccttcgatt ggtcgtccgt ttgtgttctt tacataaaaa gataaaggat agtttcgctt
     1321 agcgatggcc aaagaatcta aataagcgat gagtagccaa actaagccga ttgatccaag
     1381 tagtataccg ataaaccaaa taaaaaattt ccattttttc cattgatcaa ttccttggat
     1441 cgcttttttt gcctcttttg cttgataagg aatacgatgc cccgtcacaa gcagtcgatg
     1501 tgtattgacc atataaggag tacaggtaag caaagtcacg agatctctgc ctttttggat
     1561 acccaaagaa tctacttcat caggtaagac cacttggatt ttttctactt gatacgccaa
     1621 tgtctttcca ttgatctgga tgaaaaactg gtctcctttt ttcaacttag gcaaatctgt
     1681 aaataatttt gcttctggga gtccacgatg cccagaaaga acggcatggg tgtttttccc
     1741 gccagtcgga taagaacttt cttccaataa tgacgttcct ttttgcaaaa atatttccgt
     1801 tgtctgatcg aatataggta agctgacatt gatttttggg atttcgatga ctccaagcat
     1861 atgttccata aaaaaggcat tactaggtaa atcttttaat gcttttgcat caactgcttg
     1921 gttgaaactg cctatccctg gtgatacgtt ttgctctgcc aactgttggt ttttctttgt
     1981 cattttttcc tgctgttttt ttatttcctc tgaattctta ttcgatgctt gcttttgata
     2041 atggatgatc atttgctggg caagaaaatc attcagtgaa tcttctacaa aaggataaga
     2101 aaagatcccg atcccaacga ctaaaatgat tgccatgaag atgtccagta ttcttattct
     2161 gcttttattc atcatgtcca cctattttct aatatgtata tgaagaggtt gtgaaatcag
     2221 cgc
//
LOCUS       DAKBVG010000266         2214 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000266 DAKBVG010000000
VERSION     DAKBVG010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.268"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(102..626)
                     /locus_tag="OUD09_002574"
     CDS             complement(102..626)
                     /locus_tag="OUD09_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCT9693797.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(619..1092)
                     /gene="tsaE"
                     /locus_tag="OUD09_002575"
     CDS             complement(619..1092)
                     /gene="tsaE"
                     /locus_tag="OUD09_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCT9693798.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1215..2195)
                     /gene="pta"
                     /locus_tag="OUD09_002576"
     CDS             complement(1215..2195)
                     /gene="pta"
                     /locus_tag="OUD09_002576"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCT9693799.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
BASE COUNT          566 a          537 c          365 g          746 t
ORIGIN      
        1 tcaaaaaaaa tggactgcga cacgactttt gtcacagtcc cttcgtgtgt ttttctgcct
       61 tctcaaaagg atgacagttt ctcactcttt tcctatttct tctattcaat caaataactc
      121 attttatata catctaaaaa ttcacctaga tctgttcttg cccctctagg catgaccgct
      181 tcgatttgga agcccatttt tcggtaaagg tgtacggctc tttcattacg aacttgaacg
      241 tcaagctcca atcgaaaaag tacacccatt tccttcgccc aactgatgat ttcttctagc
      301 ataagagtgc ctagtcccat tccccaatat tcctgcagaa tactgatacc gacttctccc
      361 acgtgagaca aacgaaattg actgtctgcc ttgacagatg ctgttccaat aatcgtccct
      421 tcatagattg caagcagcaa taaattatta ttgctttcgt atatataatc caactcttct
      481 tctaaggttt ctggtggtaa caggagttct gcttcatcca aaactaaaaa ttctgtctcc
      541 ttattaactt gtcccatcat caaaagaatg tcttttgcat ctcctggcac tgcttcccgc
      601 aagatgatcc caacttcttc actcatgatt ttcagcccat ttttctagaa ttcgctgttt
      661 gaaggtttca ctttgctccc cgtatgagat tagtttcaca taccgctttt ctaaatctgt
      721 atcacttttt tctaaaatca gttccaaata atcctccggc agtgcttctt ctaatagatt
      781 tccccattca acaacggaaa gcccatcacc ttcaaaatat tcatctaacc ctaaatctgc
      841 tccgctagct gctacccgat aaatatccat atgatacaac ggaatccgac cttgactata
      901 ttctctaata atcgtgtatg tcggactttt gatcatttgc gttatcccta atccttgggc
      961 aattcctttc gtcaacgttg ttttacccgc acctagatca ccagtcaata tcagattgtc
     1021 acctggcatt gcggaatgac caattgcctc tgcgaattgt tctgtcattg ttaatcctga
     1081 tagttcgatc atttttcatc ttctcccgct cttctttctt tttgccagta tatcgcgtaa
     1141 tgaagttttt ttaaagaaaa aatgaactag tcaagaacag cagctgctgt ttttggctag
     1201 ttcgcgtgta tttgttaatc aatcaatgtt tgtgctgctg tgataatcga taatttatat
     1261 acatcttctt catttgctcc acgagacaaa tcagaaaccg gtttgttcaa gccttgtaga
     1321 attggtccga ttgcttcaaa gttaccgaaa cgctgagcaa ttttatagcc gatatttcct
     1381 gactgcaatt ctggaaatac aaagactgtt gcttgaccag ctacttctga atctggcgct
     1441 ttcagttgtg caactgaagg aacataagaa gcatcaaatt gcaattcgcc gtcgatcatg
     1501 tattgtggcg ctagtttttt agcgatttta gttgcttcaa ctactttgtc tacttctggc
     1561 gatttcgctg agccttttgt cgagaagctc atcattgcta cttttggttc gatatcgaac
     1621 aattctgctg tttttgcaga atctacggcg atttcagcca attcttgcgc agttggattc
     1681 acgttgatcg cacaatccga aaagacatat ttttcttgat ctcttccacg taccatgatc
     1741 atcgctccgc ttgtccgact gatgccaggc tttgttttga tgatctgaag tgctgggcga
     1801 acagtgtcgc cagtcgaatg gatcgctcca gagaccattc cgtctgcaat ccccatatac
     1861 gtaagcatcg taccaaagta attcacatct tttaaaatct tttctgcttg ttctttggat
     1921 gcctttccat ttctacgttc aacaaaagta tccaccattt catcccactt atcgtaatta
     1981 tcagggctga tgatcgtaaa gctagatgtt ttgattcctc tggcatgcgc tgcatttctt
     2041 atttcttctt cgttccctac taaaatgggt tccacaagtt cttctgcttt caaccgggca
     2101 gctgcgccta aaatacgagc atcagtagct tccgggaaaa cgattttgat cccacggcga
     2161 acaattttaa atcttaaact atcaaacaat tccacaagat gacctccaga cgtt
//
LOCUS       DAKBVG010000267         2202 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000267 DAKBVG010000000
VERSION     DAKBVG010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.269"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(<1..543)
                     /locus_tag="OUD09_002577"
     CDS             complement(<1..543)
                     /locus_tag="OUD09_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCT9693800.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTA"
     gene            complement(674..1258)
                     /locus_tag="OUD09_002578"
     CDS             complement(674..1258)
                     /locus_tag="OUD09_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCT9693801.1"
                     /translation="MESGLSHFEKVGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(1589..1822)
                     /locus_tag="OUD09_002579"
     CDS             complement(1589..1822)
                     /locus_tag="OUD09_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HCT9693802.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLTKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(1837..>2202)
                     /locus_tag="OUD09_002580"
     CDS             complement(1837..>2202)
                     /locus_tag="OUD09_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032488777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HCT9693803.1"
                     /translation="SKFASIPNDKKMIVTSEGCFKYFSKAYNVPSAYIWEINTEEEGT
                     PDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDTNIPIYSTIFTDSIAEKGQDGD
                     SYYAMMKWNLDKIAEGLSK"
BASE COUNT          758 a          381 c          287 g          776 t
ORIGIN      
        1 agcggtcatt tcttcagcaa tcatagttgc acgtataaga acgggaacta acagaaattc
       61 caaatattgc agaggctgaa aaaaagacaa gcgaatgttt ctcattttta aagattcttt
      121 aatcattctt atttcagaac gataagtcgg aaagaatctg atcactactg taatacttaa
      181 cattattttt ttcggtaaat gtattttttc taaagaggat aataattcat tgctagtata
      241 gacactaacc aacaaccatg agatcattac gaccggcaac attcgaaata caatatataa
      301 ggatgtccca aaaaatatag taaaaaactc tatcttgata gcatcaataa ttttcattac
      361 taaaaacaga actgaataaa tcaagatgaa ataaatacat gtctttattt tacgaaacat
      421 caatactaaa actgtagcaa taatgaaaca ccccacttga aatatatttc cctttcctaa
      481 aaaaataatg accggaaaaa aaatatttaa agtaatcaag gttaacggat tgatttttga
      541 cactctttca cctccaaaaa ataagatcgc actgttatct taagctgata aaaatgcacc
      601 gttattcaga taacagtgcg atcttatcaa aaagcataac tgagaacaat tctcaatgca
      661 agcttttttt taattaactc agtaattcaa tccacccagg aacagaaaat tgatacaatc
      721 ttctggttaa tagcatagtc ttttcttttg aatagctatg cttcaatata tcatagaatg
      781 tagcgtagaa tgagtaaatg tagaagtgga tttcttcttc attaaagact aaattttgat
      841 tgatcattcc gatagtcttt aacttatcta gacaacagac tgtctttttt gtcacaaatg
      901 atgtaatttt ttcaacaata tcttgatatt tggttccagc tgattggaat aaaaccaaat
      961 cgaatatacg tttattctca aaaatataat taataaatgg atctaattca ttgaagttct
     1021 ctctccaact tttctctata tctatatttt ctaagtttaa gaggtatttg gtaaactctt
     1081 cattatacat attaataagc gcattaatat gcggctgaat aatttcttca aataacgctt
     1141 ccttagaagt aaaatgttta taaaaagcac ccgtagttac gcctgcggtc aagcacagtt
     1201 gccttatctt agtttgctga aatcctactt tttcaaaatg gcttagtcca ctttctatta
     1261 atttttggtg agttgtaata taatctctgg tttctatggg tacttcctcc taataagatg
     1321 aattaactat attatcttac acaggacttg aagtttcaat cgttaacgtc tattactgaa
     1381 ctcattcctt agagctaaac taatgagaaa gagcaaaata actgatacaa aaagcaatcc
     1441 tacaagaatt aatagactca tctaaatcat tcaccttttc taaaataatc cgagaaagat
     1501 ttaaaaaaat tatatccagc tattaggata agtcctatca taatcactcc aatagccatt
     1561 ttcttatcct ccctaaaaat aaagcctttt acttaaaacc ttttttgtaa tcgtcaataa
     1621 attttttccc tttatcagtt aattgaaatc ctatatctaa atagtcctca tatctaagtt
     1681 tagtcagcat ctcacttaca gatgctggac taacacttag aacttttgct aaatcattat
     1741 tttttaattt cttttttctt tttgataatt cctgaattgc tagtaaataa attagtatag
     1801 tagttgtttt aatttgtacc atacctaccc tccaaattat ttcgaaaggc cttcagcaat
     1861 tttatccagg ttccatttca tcatcgcata gtaactatca ccatcttgtc ctttttctgc
     1921 aattgaatca gtaaaaatcg ttgaatagat aggaatattg gtatcttttg atactgtttt
     1981 cattggtcta tcgtccacac tgctttctac gaataaggag ggaacttttg ttgtgcgtaa
     2041 tttttcaact aagtgtttta tttgatctgg tgttccttct tcttcagtgt tgatttccca
     2101 aatgtaagca gaaggcacat tatacgcttt cgagaaatat ttaaagcatc cttcacttgt
     2161 aacaatcatt tttttatcat tcggaattga agcaaattta ga
//
LOCUS       DAKBVG010000268         2193 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000268 DAKBVG010000000
VERSION     DAKBVG010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.270"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(345..800)
                     /gene="tnpA"
                     /locus_tag="OUD09_002581"
     CDS             complement(345..800)
                     /gene="tnpA"
                     /locus_tag="OUD09_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HCT9693804.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1043..2193
                     /locus_tag="OUD09_002582"
                     /pseudo
     CDS             1043..2193
                     /locus_tag="OUD09_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
BASE COUNT          695 a          431 c          415 g          652 t
ORIGIN      
        1 tcatcaatac ttacgtaact caaaaatact tccaaaggtg ttcgataatc cagtgatttt
       61 ctaggaagat tatttctgcg ccagcatttt ctaatgtttg aatttgtgtt ttgtaatctt
      121 ggttgcttgt gctaattctt gaatagccgt aaattgccaa aatttcatca ccctttctgg
      181 acataagttt ctgacaccag ttaaaatcaa gtctatcagt aacccataca cccgtgagga
      241 cgggtgtatt tttaatcccc ctagaagggg tcgacactcg ctgcccctta aaaggggatt
      301 tttaaaagac cgccaactgc tcatcttcac tcacttactt ctctttattt tttaaatggt
      361 tctacgtact ctcttttact tataatatat cgaatgcgat catcagcttc ttgttcacgg
      421 atgtattttt catttgtctt ttgattcaat ccgactgtac tcacatagta tctcttcgac
      481 caaaaggatt tgtttccgta tttatatttg agatttgcgt gctgctcgtg gattagtacc
      541 gcactacgac ctttcagata ccccataaaa ctggatatac atatttttgg tggtactcga
      601 acaagcatgt gaatatgatc tagcattgca tgtgcttcta tgatttccac atctttcatt
      661 tcgcacaact tcctaagtat ttttccaaga tcttttcgta attgcccata gattactttt
      721 cgttgatatt ttggtgtaaa aataatatga tacttacaat tccaacgagt atatgctaaa
      781 cttttatcgt catttgacat gaaaaaactc ctttcgatct gatgagcggt tggtagccac
      841 tcactagtat atcgaaagga gtttttatcg ctaaagatac ttcttccacg cgtagaaacg
      901 cgcggttttc ttgtttcgca cgctttacgc acaaaactga ctgaagtcat taagaaaata
      961 aaagggtcta aaacttttaa gttttgtacc gacccccaaa agttagacca aaaaatctaa
     1021 cttttggagg tcggtatttt ttatggctaa gtatactttt gaacttaaat taaagatcgt
     1081 tcatgattac ttggatggta aaggcggaag tgattatctg gcaaagaaat attccattaa
     1141 ggctccatca caagtgaagc gctggatcaa tgcctatcaa gaatttggtg aagagggtct
     1201 agtaagaaag cgacaaaaga aaaaatattc tgttcaattt aagctggatg cgatagagtt
     1261 atatctaaca agtgagctgt cctatcgtga agttgccaac acacttaaca tgaacaaccc
     1321 taatttgatc gctagttgga tgcgtcaatt ccgtgagggt gggatcgatg gactctcaaa
     1381 aacgaaagga tgtccaccta tcttgtctaa gaaaaatgaa cccaaaaata agaaaaaatc
     1441 tacaactaaa gcaacgtcta aagaacgtga acgcatcgag gagttggaga aacgagtccg
     1501 ttcccttcaa attgaaaatg cctttttaaa agaattgagg aagctgcgaa aacaggaagc
     1561 ccaacaacga cgaatgaagc aatcgcacga atcatcgcaa gcctccgagg accgtttaaa
     1621 ttagttgagc ttctagaaac tttaaagttc cctaaggcaa tctttatgta ctggcaaaaa
     1681 cgattggacc gtaaaaatcc agatcaagag attgaagcgg aaatgcttaa agttcgggag
     1741 aagcacaagg attatggctg tttacggatg accaatgaac taagaaaccg cggtttctct
     1801 atcaataaaa agaaagttca gcgcctgatc aagaagctag gaataaaagt aacgacatac
     1861 acacgtaaat cacggcgcta caattcttat cgaggacaag tcggagcagt agccaaaaat
     1921 cggatccatc gtcgtttcta caccagtatc tgccatcaga aaatcacgac ggatacaact
     1981 gaatttaaat accatgaagt ggatgccaaa ggcattatcc gtcaaaagaa attgtatcta
     2041 gatcctttta tggatatgtt caatagtgag attttatcct atcgtatctc tgaaaagccc
     2101 aatgcattag cggtaatgga aggtttggag gaagctatcc agaccactaa cgattgtccc
     2161 tatcgtcgta cgtttcactc agatcaaggt tgg
//
LOCUS       DAKBVG010000269         2180 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000269 DAKBVG010000000
VERSION     DAKBVG010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.271"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            208..1131
                     /gene="truB"
                     /locus_tag="OUD09_002583"
     CDS             208..1131
                     /gene="truB"
                     /locus_tag="OUD09_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCT9693805.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1135..2076
                     /gene="ribF"
                     /locus_tag="OUD09_002584"
     CDS             1135..2076
                     /gene="ribF"
                     /locus_tag="OUD09_002584"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HCT9693806.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKKDEQATAEYFSQQ"
BASE COUNT          777 a          353 c          471 g          579 t
ORIGIN      
        1 ccaatcaaat ggtgcaaaga aaaaatcttc cattctattc cctcctttaa aaaaacataa
       61 catgatttct atttacgtga gcatgaattt tgttacgttt ttattgccga aaaataaaaa
      121 ccgttcatta tgatgtcaaa ttcgtcagta attgatgcaa tgtgttataa taattcagtt
      181 aaatcaaaaa tgaaatggag aaaagacatg gacgggttat tacctttatg gaaagaacgc
      241 ggaatgacaa gtcatgattg cgtattcaaa ttaagaaaaa tcttacatac gaaaaaaatc
      301 ggacatgggg gtacgcttga cccagacgta gacggtgtgt tgcctatttg tattggaaaa
      361 gccacaaaag ttatcgagta tttgacagat tctgggaaaa tctatgtagg cgagataact
      421 ctaggtttca gtacaacgac ggaagatcga tccggagaac gggtagaaac aaaaccagta
      481 gatatgtctt tatctgaaga aaaaatagat gaagtgatgg cttcatttat tggtgagatc
      541 acccagatac caccaatgta ctcagcggta aaagtcaacg gtaagcggtt atatgaatat
      601 gcccgcaata atgaaacagt ggagcgtcct gttagaaagg ctcagattta ccgttttatc
      661 cggacaagtg aattgacaag agacgaagtg acaggaacca tttcttggcg cttcgaagta
      721 gaatgcggga aaggaaccta tgtccgcaca ttagctgtag atacaggcaa gaaacttggt
      781 taccctgcgc acatgtctga tctgacccga acagcaagtg ccggtatgtc ccagaaagat
      841 gctctgacat tagaagaagt agctcaagct gtgaaaaatg ggacaatcga tgattgtttg
      901 ctgccaatag aaaaaggagt gaaacgattc aaacgagtag atattactga tgaagtatgg
      961 cagaaagtaa aaaacggtat gagacttgat tatactgtat ttgcgctccc agaaatgccg
     1021 tcagaagaaa ttgctttgtt ttataaaggc aaagttgtca gtatctatca gccaaacccg
     1081 aaagaaaaaa acaaattaaa acctagtaaa gtattgagaa atgaggaata acgaatgaat
     1141 atcatcaaga tcagacatcc ttaccaatca gaacaaattc cagaagacga tgtcgtttta
     1201 gtactgggat tttttgacgg cgtgcatatt ggacaccaga aagtcataaa gacaggtatg
     1261 gaaattgcac aaaaagaagg attgaaatta gcattgatga ctttcaatca gcatccatcg
     1321 attgtcttta aaaagatcga tcctagcagc gtgaaatatt tgactagcct taagcaaaaa
     1381 gaagaaaaaa tggccgcttt gggtatagat tatttatatg aagttgattt tacttcttct
     1441 tttgctcatt tagcaccgca ggaatttgtt gatcaataca tcgtagggtt acatgcaaaa
     1501 tatgctgttt ccggttttga ttatacgtat ggaccaaaag acattgcaga tgtcgctcat
     1561 tttcctgaat atgccaaagg aagattcaaa atcgtcacag tttctaaaga agaagaacaa
     1621 ggtgaaaaaa tcagctctac ccgtattcga ggattactag acagcggaca agtaaaagaa
     1681 gcagcaaaac tattaggttg cacatatgag gtagaagggg tagttgtcca tggcgatgca
     1741 cgaggcagac tactaggatt ccctacagca aatgtcaaag taaagagcac agttcatttg
     1801 cctaaagaag gtgtttatgt atcggaaata aaaattgggg aaacttggta tccagccatg
     1861 ggttcaattg gacataatga tacattcggt gaaggacggg agctgactgt agaattgtat
     1921 attttggact ttaatcaaga tatctatggt gaacatgtcg cagtacgctg gaatcaattt
     1981 ttaagagatc aagtgaaatt ttctagtgta gaggaattga ttgaacaact caaaaaggat
     2041 gaacaagcaa cagctgaata tttttctcag caataaaaca ttatgcataa aatctcttgt
     2101 ttatgtataa ctagcaattt caataaaatg ggtgataaat aagcccagga aaggggctgt
     2161 gacaaaagtc ttgtcacagc
//
LOCUS       DAKBVG010000270         2169 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000270 DAKBVG010000000
VERSION     DAKBVG010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.272"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            230..1339
                     /locus_tag="OUD09_002585"
     CDS             230..1339
                     /locus_tag="OUD09_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HCT9693807.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNVILSGN"
     gene            1422..2169
                     /locus_tag="OUD09_002586"
                     /pseudo
     CDS             1422..2169
                     /locus_tag="OUD09_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338086.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
BASE COUNT          737 a          322 c          462 g          648 t
ORIGIN      
        1 cgacaaaaca ttaagtaaac agttatggtg ttgttaacaa aaagaaaacg cattcaaaac
       61 aggaggaatt aagatgaaac atttcaaaaa aatagcttta acagggatag ctgttttaac
      121 tgggacatta ctcgcagcat gtggtggggg agatagtaat tctaaagacg ggcaagtaac
      181 tttaagctat gcgagttgag atactgacca agctccagga ctaagaaaaa tgttagatga
      241 atttgaaaaa caaaatcctg atatcaaagt agaaatggag acaacgcctt gggatcaata
      301 ttgggttaaa ttagaagctg caacaacggg aggtaatatg ccagatgtag taaccatgca
      361 ctctagcgaa tcctataaat atatgtcaca gaatgctttg atgggacttg atgatgttat
      421 caaagaaaat gatattgaca tgggaaactt tactgaagga attgctgatt tctatacgct
      481 tgaagacgaa ctatacgcaa tccctaaaga tgcttctgta gtggggctat ggtacaacaa
      541 agaattattt gataatgcag gtgtcgatta tccagatgaa acatggacat gggaaacact
      601 cagacaagct gcaattgatt taacagatga atcaaaaggc atctatggtt ttgcatcaca
      661 tattggaaca gagactggct actggccatt tatttatcaa aatgggggag aagtatttgc
      721 cgatgacaat acaaaatcag gtttgaatac cccagaagca caagaagctt tagcttttta
      781 tcgagatatt gcacttgttg atcatgcttc accaacaatt acagaattac aagatcctga
      841 taagttaagt cgttttcaat caggtagatt agcaatgatg attgaaggaa actggtttgc
      901 accgggattt ttgaaaaatg actatctagt tgaaaatgtt gcttctgccg tgttacctaa
      961 gggaaaagtt aatgcaacaa ttacaaatgg acttggttgg gctgccagtg cgagtacaaa
     1021 atacccagaa gaagtgaaaa aactaatggc atttttagct agtgaggacg ccaataaaat
     1081 ccaagcggaa acgggggcat caatcccagc actaaaaggc tttgaagacg agtgggctgc
     1141 ttcttcagat acaattgatt tgagtgcttt tggaaaaatg ttggagtatg gagtccctcg
     1201 tccatacaat aaagatggat tacgagctga acaagcagaa gttcaaatta ttaaccgtat
     1261 tctgtctggt gaagtaactg ttgaagatgg aaccgctcaa attgaaaaag aagtcaatgt
     1321 gatattgagc ggaaattaaa ttagttaatt atacaatata caggcgaaag gattctattt
     1381 cgtctgtata ttaatggatg aataccaaaa agggggatat tatgaaaaat cgaatttttg
     1441 gaaaatatcg taaagatcaa gtgatatggg gctatatttt tatttttcca acggcattag
     1501 gattaatttt attaaatata tggccagcaa ttcaaacagc aattttaagt tttcaaaaaa
     1561 cggtagggtt cggtgatact gaatgggtcg gattaagaaa ttaccaaaaa atgtttgcag
     1621 acggtgaagt ttttcagtct ttattaaaca cattaattta tgcagtggtt tcgataccta
     1681 gtattgttgt attatccttg ttggtggcag ttttaatgaa tagaaaagtt aaaggattgt
     1741 cgatttatcg gacaatttat tttctaccag tcgttgctgc accatccgca gtcgcaatga
     1801 tttggcgctg gatgttcaat aatgattatg gactgattaa taacatgttg gctaaaatag
     1861 gtctcgatgg accagcatgg ttaacggatc catcgattgc gatttattct attatcattg
     1921 taggagtttg gagtgctatt ggttataaca tggtgttatt actagccggc ttataagaaa
     1981 ttcctaaaga ttattatgaa gcagcggaaa ttgatggtgc tagtccaatc cagcagtttt
     2041 tcaacattac gttaccatta gttacaccaa cattattttt tgtagttgta acaacagtta
     2101 tcaatgcttt ccaagtattt gatgttattt ttatgatgat tactcctaat agtaccgcca
     2161 tgtttaaat
//
LOCUS       DAKBVG010000271         2137 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000271 DAKBVG010000000
VERSION     DAKBVG010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.273"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(66..296)
                     /locus_tag="OUD09_002587"
     CDS             complement(66..296)
                     /locus_tag="OUD09_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693808.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            complement(390..728)
                     /locus_tag="OUD09_002588"
     CDS             complement(390..728)
                     /locus_tag="OUD09_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693809.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            complement(1656..1772)
                     /locus_tag="OUD09_002589"
     CDS             complement(1656..1772)
                     /locus_tag="OUD09_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HCT9693810.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
BASE COUNT          697 a          380 c          292 g          768 t
ORIGIN      
        1 acagcaaaga tatcgaaaaa acaataacat aatatgtgtc aaaatttttc ataataagaa
       61 ctcccttact gttgcatact aacataatcc aaaaactctt ctacttcagc taaagacatt
      121 ccactttctt tagtccaaaa aacagttttt tctatcgatc cttctctgta aactgtaaat
      181 gtcggagtcg agttaattga gtagcgactc ttgaattctt tccactcatt ttcatcttct
      241 ctaacttttg ctacattcac gtaataaata ttttcttttc ccttttctac cagcatatcg
      301 aatagcttcc tgcttttgat gatcgatttc ttgtgctaca tctagtaaat tttcatcaat
      361 ttctatatca taaagtattt cactagtgtc tacatccaca atatatactt tttatcagta
      421 gcaatttttg agtaatccgt taaaatgatc tcagtctcgt cactttcatc agtataaata
      481 tacgttaaca atgaactcac cttttcaatc ggtgttagct ctaagcctac tgtatttttt
      541 actattgagt cattatttac caccataact tcgtactgta tataccatcc tatttggttt
      601 ccatcaatat cagtaattgg ctcactagct aatggagtct ccggaacaat attttcctga
      661 tatttcttaa actcttctgt agcacgaaaa aggttatcat gttcctcact tgaaactggt
      721 tgagtcatag aaaatgttac ttggctaaac agagggaaaa taaacactac aatcaacaaa
      781 gtataaaaaa aagcccttct ttttcatttt ttatcttcca atcacaaatt gaaaaactta
      841 ttcctgcaac tttgagtcta gtatcacaat cgactgacta caactattca tttacattct
      901 actttaagtt cctccttaac cattaatttt attaaagata cttgtaatag tgattctcac
      961 aaaatttcat gaaaaatact ttattactgt atctaaaatt ccaatacaca acttatcgac
     1021 tattatctga aaaccaaaac ttattattcc ttgatgttat tctagcaata gtaactgctt
     1081 ttttattttt ttgcatattt tttcggcgtt actttttaaa gttaactttt tcgacaaata
     1141 gaaagagata cgaatcactg atttttttga agaaaagaaa tggagtgagc tgaacgttag
     1201 agatattggg agctttcgta actgcttatt aaacgataat ggttatttgc aaccgtattg
     1261 ctcgcttcta catgaaatat ttcatgaaat atttcgcaaa acctttgatt atgtagttac
     1321 gtttccattc ttgactaaaa atgtttcaaa aacgaacaaa ggtgttaaaa ggtaaagtga
     1381 tcaatactac tggtcaaaat aagaggttga aagggaattg aaaaataact acagataata
     1441 cttttataaa cgtacgtgct ttgtggttct gtagttaact atataactgg aattagggaa
     1501 tggtatcaat ttgaaaagaa agattctacc aatttatcat atgttaaaaa tggaaaatca
     1561 acagcagttc tttcggaaac cttacacaaa aaccgattat tgaaaatagc cgaaaaacgt
     1621 ttttcggcta tttttttaat taagtttcct tcttcttacc ttttcccact attccgtttc
     1681 acatctttga tgatcttcag cgcacgtttt cgtgttttga tatttgggct tttcaagcgg
     1741 cggtatgcac ttgagatatc tgttttttcc atatggacga cccactttct tctttgattg
     1801 tagtcatgct aaaaaacaca aatcgaaaca attacgattt gtgttttaaa tggtatcaaa
     1861 agttaaaatg aaagtcaact tgttaaaaca gtcgatcaga ctagctgatt gactcctatc
     1921 tctggaaata aattttcatt attacgtttt agctacctaa aaaaggccct cttgtccgtt
     1981 aactttaata tctattgtta tagctagttt acctattgaa cgtactgtgt gttcgataga
     2041 acactaaaaa agaggctggg acagaagcgt ttaactccaa gaaataagaa gaaattcacg
     2101 aaaattgctt ttcaaatttt tgtgaatttc agcttat
//
LOCUS       DAKBVG010000272         2130 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000272 DAKBVG010000000
VERSION     DAKBVG010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.274"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(<1..914)
                     /locus_tag="OUD09_002590"
     CDS             complement(<1..914)
                     /locus_tag="OUD09_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HCT9693811.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNHTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="OUD09_002591"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="OUD09_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCT9693812.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT          722 a          377 c          411 g          620 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatggttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatatgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caattcttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa accagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaaaaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg
//
LOCUS       DAKBVG010000273         2095 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000273 DAKBVG010000000
VERSION     DAKBVG010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.276"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            <1..2046
                     /locus_tag="OUD09_002592"
     CDS             <1..2046
                     /locus_tag="OUD09_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HCT9693813.1"
                     /translation="NPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEHFYTS
                     SKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWLTKNY
                     GWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEGIGWY
                     GADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKATQKQV
                     DSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTALSNAK
                     KVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYTVTINHIDNQTGQVIATDQAK
                     VTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQINLLC
                     NNNYKNEGRVNILKEQIVNVTHGDSVTLTAPSIQGYVLDDRVEPTNSVTLNNVTESQN
                     YIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFATPWKNMTDQNYFLCRNDDPT
                     VTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVAPMQF
                     NDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYIDDLLS
                     DDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANPIFK"
BASE COUNT          745 a          388 c          385 g          577 t
ORIGIN      
        1 aatccaaaaa ctgttttagc tattggaaca tttgctgcct cacttagtct tgctggatct
       61 gttgacgctg cttcaactaa cgtcatgtat cgcctttaca atccaaataa taatgaacat
      121 ttttacacct ctagcaaaaa agaacgtgac catctagtaa agataaaatg gggaaattat
      181 gaaggccccg cctgggaagc accgactagc ggtggccatc ttgtctatcg tttgtacaat
      241 aaaggtcttc gtgatcacca ttacacagct agttgggatg aagtgaaatg gttgactaaa
      301 aattacggct ggacttatga agggcctgcc tggcgttctg ccgaaaaaaa taataaaccg
      361 atctaccgcc ttttccttcc tggtgtaaaa tctggctcac atcactacac tgcaagttgg
      421 aaagaagtac aatggttaac caaagactat ggctggaaat acgaaggtat cggttggtat
      481 ggcgctgaca cctctaaacc caaagtgaat aaatcggctc tagaagctct ttatacgagc
      541 gttaagggaa cgaaaaaagg tgactatacc gacaatacct ggaacgcatt ccaaaccgct
      601 ttaaacaatg ctaagaaagt gttaaatgac tcaaaagcga ctcaaaagca agtggatagt
      661 gccaaaaata aattagattc tgcttacaaa ggattaaaga aaaaaccagt tcctactgtg
      721 aataaagctg aattacaagc tttatacaac cgagtaaaat caactgctaa aggagatttt
      781 acagaagaat cttggaataa ttttcagact gctttatcca atgctaaaaa agtactagac
      841 gattctaagg tttctcaaac acaagtaaac aatgctaaaa atagtcttga tgctgcattt
      901 aaagggttgc aacacaaacc tgaaacacca aaagaatata cggttactat taaccatatt
      961 gacaatcaaa caggtcaagt gattgctaca gatcaagcaa aagtaacttc tggttctact
     1021 tacactgcta aggcaaaagc atttaaatat agcgaaggcg ctaaagataa ttttagctac
     1081 aaagtgattg gtaatgatac tcaaagcaaa acaatcacag gtaatacaac aatcacattc
     1141 aattataatc aagtgcatca aatcaatttg ttgtgcaata acaactacaa aaacgaagga
     1201 cgagttaata ttttgaaaga acagatagtc aatgtcactc acggtgacag tgtcacttta
     1261 actgctcctt ctattcaagg atatgtttta gacgatcggg tagaaccaac aaattcagta
     1321 accttaaaca atgtgactga aagtcaaaat tatattttca actacactcg taaatttaat
     1381 gtgactgtta atcatgtaaa tgctgatacc aacgctgttc tctctagtga atcaaaaaca
     1441 gtatacgaag gggatagctt tgctactcct tggaaaaaca tgactgatca aaattatttc
     1501 ttgtgcagaa atgacgatcc taccgttacc gtggataaaa acggccaacg atccattaat
     1561 aacgtcgatc aaaataaaac aatcacattc aaatataaaa agatttcatt agacgaatta
     1621 cgtaacttta tgcgtcaaaa agaacttgct tggttaaatg attatcgcca acaaaatggt
     1681 gttgccccaa tgcaattcaa tgatattgtt caacaggctg cagatattcg agctaaagaa
     1741 ttacaagtca gcttcagtca ctatcgccct ggtggtggta ctttccaaga tttattggaa
     1801 agcttaggct gctacggtgc taagggagaa aacataagtc aaactgggct gtatattgat
     1861 gaccttttat cagacgatgg tgctatcgat gccatggtag gatggaaaaa tagccctgcc
     1921 cataatgcaa atttacttta taataaccaa agtattctcg gattaggtca taattttgta
     1981 gttgactctg ctggtagaat tggcgtagat aatgtattta ttagtgcgaa tccaatcttc
     2041 aaataaaaga aaacaaggct gattctcagc cttgttttta tatttatttg agttt
//
LOCUS       DAKBVG010000274         2091 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000274 DAKBVG010000000
VERSION     DAKBVG010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.277"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            71..928
                     /locus_tag="OUD09_002593"
     CDS             71..928
                     /locus_tag="OUD09_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HCT9693814.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            929..1477
                     /locus_tag="OUD09_002594"
     CDS             929..1477
                     /locus_tag="OUD09_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693815.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            1737..>2091
                     /locus_tag="OUD09_002595"
     CDS             1737..>2091
                     /locus_tag="OUD09_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693816.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQIEYVPNELIVSLKATQRISTEKNKPQNLLGVKVMVEKDLTLRINESDYNKRDQVLL
                     VKLNEEISVEEAVRTF"
BASE COUNT          807 a          268 c          446 g          570 t
ORIGIN      
        1 ggtagtaact aaaaataaat atgttataat agtatcaaat gaatattgct aaaagaaaag
       61 aggtaggaac gtggtagcga agaaatatgt aatcggtaat tttaaaggtg gcgtaggtaa
      121 aagtacgtgc gcgcaaatgt tagggtttga atcagcagtt aatgaaggaa aaagaacctt
      181 gataattgac ttggatatgc aaggaaatac ttctgatgtg atgaacttaa cgcatatgaa
      241 cttcactaac gaagaaggag gcggagaagg tgaaccgatt gtttacgaaa atactgtgac
      301 tgacgtgttg ataaacaatc ttccacctaa agaggctgtc tataaagtaa tcaataacct
      361 ctatatactt ccagcagaca tgtcctttga aatgtatgat gattggatca aagaccaata
      421 taatgattca ttatcccaat tcaaatatat gaaagaaaag ctagaaccat tatttgatga
      481 gtttgacgtc atctatttag atgtcccacc ttctatttct gtatatagta aatccgcgat
      541 gtacattgcg gactgggcaa tcgtcgtctt acagacccaa gtaaaatcga tgagaaatgc
      601 gatgcaatat cttgagtata tggacttttt tgtgaaagaa tttgatacaa atttatatgt
      661 tgctggtgtt attcctttca tgttggaaag cggtgacgca gtagatcagg aaatgtataa
      721 acaagcccaa gagatttatg gggaacattt gattaaaaat gttgtactca aaaatgcgcg
      781 cttaaaacga tacgacggat caggtattac gatggagaag acaaagaaag gtctcttaaa
      841 acaatgggat cgcaaaagcc acgaattatt tatcagtatt ttgaaagaat tggaagaaca
      901 cgagaattgg tatgcggagg tggagtaatt ggctgacatc aaaggacttg atatttttgg
      961 gaacaacaaa ggactcgcca aaataaaaaa agaagcagaa gcaaagaaaa agcagcaagc
     1021 aattacacca tctgaaaata agcaattaga agtgcaccaa gttcaagaaa taaaaacttc
     1081 ctccaatgtt gaaagacaga gtagaggaag aacaaaaaaa gttggcgcac caatcagaaa
     1141 gtttgataag gtttattcca ccaagcatcc tttaaaactg tcgactttgt taaatgcgac
     1201 ttccagagtt ttagtagaaa agtatgagac taatatgaca agagatgaat tgcttagaaa
     1261 agctttagat gagtatatta aacagaatct tacgcaagaa gataagcaag atttgtttaa
     1321 tgacgtggtg aaagaactag acttgttcag agaaaaatat ccaattgttc cagaattaga
     1381 tgatcgtgga gaaataataa gaactaccag tcagataaaa caagataccg aagactcact
     1441 gcgcaaaggg tggggaatga ataataaagt tagataataa tcaaagaaga actaagtaac
     1501 tacctttaaa cggtagttac ttagttcttc tttgatttgc gtgaaataga ggggatttat
     1561 ttttctggtt acgtgattaa aaaatccaaa aaatgagcat aggaacggtt atgattttag
     1621 ggtgtgtttt aatgagatat tgtgatttca ttaacaagtg tatgataatc tgttgttgag
     1681 ttagtaaata agtaatagag ctaactctaa aataaaacta ttaggaggag atgtagatga
     1741 aaaagtttaa aaagaaaata tatttaggat tggttgttgg ggtgagtatg tttgggttca
     1801 tggctcatac taaacaagta actgcagata ctaaccaaaa agaaaggatt atgagccaat
     1861 tagatgactc tcaaatagag tatgtaccca atgagctaat tgtcagttta aaggctacac
     1921 aaagaatttc aacagagaaa aataaaccgc aaaatttatt gggtgtaaaa gttatggtgg
     1981 aaaaagattt gactcttagg ataaatgaaa gcgattataa taagcgagat caagtattat
     2041 tagtgaaatt gaacgaagaa attagtgtgg aagaggcagt aagaacattt a
//
LOCUS       DAKBVG010000275         2058 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000275 DAKBVG010000000
VERSION     DAKBVG010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.278"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            495..716
                     /locus_tag="OUD09_002596"
     CDS             495..716
                     /locus_tag="OUD09_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693817.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKE
                     YRSAGRRNYLFSPEALKKLEKVITTKEGE"
     gene            717..974
                     /locus_tag="OUD09_002597"
     CDS             717..974
                     /locus_tag="OUD09_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693818.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTN
                     ILELNSEEQKEHKGKLIAPSYRKAAKSAVPTGFLTKQPYIF"
     gene            complement(1110..1712)
                     /locus_tag="OUD09_002598"
     CDS             complement(1110..1712)
                     /locus_tag="OUD09_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HCT9693819.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLH
                     DKDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKIRSPNPQKCANAKGMVRYFAHM
                     DNPEKFQYAKSDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDLIDYA
                     MSERFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
     gene            complement(1779..1934)
                     /locus_tag="OUD09_002599"
     CDS             complement(1779..1934)
                     /locus_tag="OUD09_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCT9693820.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANG
                     KENEQKK"
BASE COUNT          678 a          465 c          314 g          601 t
ORIGIN      
        1 aaaaaaaaac gctgaaaccc ttgggagagt aaggctaaac agctatcgac caatgaccaa
       61 tgaccaataa gggggggtgt cgtagtcccc cccctcctcc gcccaattca taaacaccaa
      121 aaccaaaaaa caaacctcag ggctccgccc cgaaccccga cctccttcta ccgaaatata
      181 tgcaggcgcc acgcttacta tttcgttcag ctatcggctt ttgcaatcta acacgaccgt
      241 ttcgctacca gtcgccttac aggctccatt ctcgcttctc ttacgtgttt taacgattgc
      301 gccgataact aaacgaaata gacgctaaat cgcttaaaac gcaaaataga ccctttttag
      361 agcattgatt ttttctacta gtcatttcga taatataaaa ttgaccacaa cacagaaaac
      421 ttatattata agcttatgtc gtaagtccga cgtactcacg taatatagac ggacgttaaa
      481 tggaaggagc ataaatgaga gtgataaaat cagaaaaatt cactttggcc gaaattatga
      541 cgaccagcga acttgcacaa gaacttgatt acaaccaagc ttacgttttg cgcttagcga
      601 aagaacatct gactgaagga aaagaatatc gctctgccgg tagaagaaac tatcttttca
      661 gcccagaagc ccttaaaaaa ctagaaaaag tcataaccac caaagaaggt gagtaaatgc
      721 ctaaagaaac aactatcaac gttgaacaac tagaaaaatt gctagaatca tattccaatg
      781 attttatcga attgaccggc ggaaaagtcg aagaaaccac cgacctcttt gacctacttc
      841 tcacaaatat tctggaatta aactctgaag aacaaaaaga gcataaggga aaactgatag
      901 caccttccta caggaaggcg gctaagtcgg ctgtgccaac tggttttctt acaaaacaac
      961 cttatatttt ttgatatcac aaaaaaatcc attttggacc tattttcggt cataatatag
     1021 tacctacttt tggtcatagt ttcgtccgta gttttggtca tagtgactaa gactttcacg
     1081 cacaaaaaaa gagctgaaat taacagctct tatcctttat ttatttttga atttacactt
     1141 ccaccatgac gattcgattt tatatattgc ccgataatat aagctgaatt atcacaaaga
     1201 agcggaaacc aatcatcaaa tctttctgac attgcatagt caattaaatc tttgatttca
     1261 gtgatattct tagaatcaac aaacgacatc atctctctaa tcaactcata tcgttctgca
     1321 ctcgttactg acaaataact agcaatttct gcaccaccat gagcgataat atcactttta
     1381 gcatattgaa atttttctgg attatccata tgagcaaaat atcgcaccat accttttgca
     1441 ttagcacatt tttgcgggtt tggagaccga atcttttgag ttatctcacg tatttgatca
     1501 tacgtctttt taccgctaaa cattatcaaa acatgccaat gaggcttttt caattcaccg
     1561 tcaggattca catctttatc atgaagtgga ctctcaaccc aaggaacatg taactcatct
     1621 aaaaactccc tccaattttc aggagccgat tcaggataga ctacaaacgt ccaattcctt
     1681 gtgcgttcat ctttagtttt tctactcgtc atgctataat caccttaatt gaacaacagt
     1741 ctaacagcaa aagcagtgcg ccaacactaa tgcttttgct attttttttg ctcattttcc
     1801 tttccgttag cattaaccca aattgtaata agaccagact tactaagacc catttgtttc
     1861 gcaattttat caagattatc tgccacttcc ttagtaagcg ttacaacgac tctctttttt
     1921 tcattactga tcataattcg tatcctccaa cgactttttc ttaaaaacca tagtaacaaa
     1981 aaagtaacta aggtgcaaca gtttagatac taattagcga ataataggcc ttaaaccctt
     2041 ggcattactg attttgtc
//
LOCUS       DAKBVG010000276         2031 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000276 DAKBVG010000000
VERSION     DAKBVG010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.279"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            <1..>2031
                     /gene="acm"
                     /locus_tag="OUD09_002600"
     CDS             <1..>2031
                     /gene="acm"
                     /locus_tag="OUD09_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HCT9693821.1"
                     /translation="AGRDISSNVTSLTVDPTNITDGGNIKVKFSFDEKKQNIQPGDYL
                     WINWPSEGNIRGEGFQKEIPLMIENKNVGTLTVRKDSAQVVFNENIKNLDSVEGWGQF
                     EIQARNVTDTGEENTGPFTVTSGDKTATVNVTKPASGSSSSVFYYKTGDMLPEDTKHI
                     RWFLNINNNGTYVEQPVKISDEIQSGQRLDPSTFEINQIHLGEQKVYRGEEGIQQFLQ
                     DFPSATFNFSVTDNYIEITIPKNFVNLRKIMVSYKTIIENPEQINFENHSEAWFKEFN
                     KPAVDGESFNHTVKNISASGGVNGTVRGELKIFKYINDTEIGIPNVTFELRRADEQPI
                     QGQSSILLTSNEQGEASIKGLQVGDYVVKEKEAPNWIDFDPLSSNELKFSINENDTEG
                     VSLPIYNKKKVTNITATKIWNGGTTPRPSIYFKLFRASTNNWEPVPDAETKRLDNGIT
                     SVTWEDIQQYDDSGNEYTFKVQEVDQNGNDYVPSGYQKIENGLVVTNENKEVISVSGQ
                     KTWEDNENQDGKRPTTITVNLLADGQPIQHKEVSEKDDWRYRFTNLPKYRDGQEIIYT
                     VTEDNVPEYSTTIDGYNIKNSYTPEKTNISIPGSHITPWKNFPEKTEREKIRNLFSQK
                     LQIFAHTGESSEQRSNYISKRAVPKQITNKSKSILPKTSSKKSSFAVIIGLL"
BASE COUNT          801 a          333 c          399 g          498 t
ORIGIN      
        1 tgcaggcaga gatatcagca gtaatgtcac ttcgttgacc gtggacccaa cgaatattac
       61 agatggagga aacataaaag taaaattctc gtttgacgaa aaaaaacaaa atatccaacc
      121 aggtgattat ctttggatca attggccaag tgaaggaaat attcgcggtg aaggattcca
      181 aaaagaaatc ccattaatga ttgaaaataa aaatgttgga actttaacgg tcagaaaaga
      241 ttctgcgcag gttgtgttta atgaaaatat taaaaaccta gactcagtag aaggttgggg
      301 gcaatttgag atacaggcca gaaacgtaac cgataccggt gaagaaaata cgggaccatt
      361 caccgtgacg agcggtgata aaacagctac tgtaaatgtg acaaagccag cttctggttc
      421 ttcttctagc gttttttatt ataaaacagg agacatgcta cctgaagata ctaagcacat
      481 tcgatggttt ttgaatatta acaataacgg cacttatgtt gaacaaccag ttaaaatcag
      541 tgatgaaatc caaagtggac aaagattaga tccaagtacg tttgaaatta atcaaataca
      601 cttaggtgaa cagaaggttt atagaggaga agagggaatc caacaatttt tacaagattt
      661 ccccagcgct acatttaatt tttcagtaac tgataattac attgaaataa ctataccaaa
      721 aaactttgtc aatcttagga aaataatggt gagttataag acgatcatag aaaacccaga
      781 acaaataaat tttgagaatc attcggaagc ttggtttaaa gaatttaata aacctgctgt
      841 tgatggagaa agtttcaatc atacagttaa aaatatttca gcttctggtg gagtcaacgg
      901 tacagttaga ggcgaattga agattttcaa atatatcaat gacacagaga ttggtatccc
      961 aaatgtgact ttcgaattaa gacgagcaga tgaacagcct attcaaggac agtctagtat
     1021 cctgttgact tcgaatgaac agggagaagc tagtatcaaa ggcctgcaag taggagacta
     1081 tgttgtaaaa gaaaaagaag cacccaattg gattgatttt gatccactaa gttcaaatga
     1141 actcaaattc tccataaacg aaaatgatac agagggagtc agtttaccta tttataacaa
     1201 gaaaaaggtt acgaacatca cagcaactaa aatttggaac ggtggaacca ctccaagacc
     1261 gagtatttat tttaaattat tcagagcaag tacaaacaat tgggaaccag ttcctgatgc
     1321 agaaacaaaa cgattagaca atggaataac ttcagtgact tgggaagata tccaacagta
     1381 tgatgatagt ggaaacgaat atacgtttaa agtgcaagaa gtagatcaaa acggaaatga
     1441 ttatgtccct tcaggttatc aaaaaattga aaacgggcta gtcgttacaa atgagaataa
     1501 agaagtcatt tcagtctcag gacaaaaaac atgggaagac aatgagaacc aagacggcaa
     1561 acgaccgacg acgatcacag taaatctatt agccgacggt caaccgatcc aacacaaaga
     1621 agtaagtgaa aaagatgatt ggcgctatcg tttcacaaat ctgccaaaat acagagacgg
     1681 gcaagaaatc atctatactg taacggaaga caacgtgccc gaatacagta cgacgattga
     1741 cgggtataac atcaaaaaca gttatacacc ggaaaaaaca aatatatcta taccaggtag
     1801 ccatataaca ccgtggaaaa attttccaga gaagacagag agagagaaaa ttagaaatct
     1861 attctctcaa aaattgcaaa tatttgctca tacaggagaa tccagcgaac aaagaagcaa
     1921 ttatattagt aagagagctg tacctaaaca gataacaaat aaatcaaaaa gcattcttcc
     1981 taaaacaagt agtaaaaaat ctagttttgc tgtaatcatt ggtttattga t
//
LOCUS       DAKBVG010000277         2004 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000277 DAKBVG010000000
VERSION     DAKBVG010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.280"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            39..362
                     /locus_tag="OUD09_002601"
     CDS             39..362
                     /locus_tag="OUD09_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693822.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            526..780
                     /locus_tag="OUD09_002602"
     CDS             526..780
                     /locus_tag="OUD09_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693823.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            850..1095
                     /locus_tag="OUD09_002603"
     CDS             850..1095
                     /locus_tag="OUD09_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCT9693824.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            1171..1536
                     /locus_tag="OUD09_002604"
     CDS             1171..1536
                     /locus_tag="OUD09_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693825.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
BASE COUNT          555 a          355 c          349 g          745 t
ORIGIN      
        1 ttattttaga aaaaatgtta ttcttttatt tattctcaat gttaggagtg attattttgc
       61 catttattgc gattttattc gatttagttg cggcagcagc ttattttctc caattaaatt
      121 atcaaacctc aggaattctg tttattggaa tgatttttca aggtgtgatt acccttttgc
      181 ttcttgtcat gatgctcagc tataaaggaa aaagatatgc ccgtattcaa acaaaagtat
      241 ttgttaaata tgtttccatt cgctatggga tcatcattct ttccttttta gtgaatgcga
      301 tcgtcctctt tttatatgta ttgaattact tagatatcaa tccattgatt ttttctcgat
      361 agataagaca cattcagcgg aacagttcct gcttatcagc agaaaactgt tctgcttttt
      421 catttttcat tgatctattc ttcttttatt ttcctagcat ttcatttgtg atgtatgcaa
      481 taacatgtta tcttttatta tatgaatcgt tgaggagtcc gttacatgat tacgttggtg
      541 agagttttgt tttgggtgcc tgcggtcgca ctggtcgcaa gtattgttta tcttatgaat
      601 tggaacaaag aacggtttta tttagctatt ttgactcttc cggctatcta ctttatgtgg
      661 aaagttttta attacaacta ctttgaaccc gatagtgttt ttatagaaga attatcagga
      721 ctggttttgt ctttattgat cgtcattctt tatttgatac gtttaaataa aaaacattga
      781 gtactattga ttgcatgtgt agctttctat agtcattata actattctac tataatgaaa
      841 ggtgattaaa tgagtatcga agaagctatt ttactggata ctttacgttg tcgtctgaat
      901 ttcttaagaa ctggccgatc tttatctcaa gtagctgttt ctagcgcgtt aagtattcct
      961 ttattcaaat atcgtaagta cgaagaagga gaagaaattc caagcatcat tgaattaatc
     1021 tatatcgctg acttttacga tatttccgtt gattacctaa ttggtcgctc agaaacagca
     1081 agcaaactga agtgattttt tccagccttg gtgctggttt tttcatttaa cgtctaaggt
     1141 taacaatcta tatataaagg agacatttct atgcgattat ggcatgaaaa attgatttca
     1201 aagcttcctc gtcaacagct tttaggacaa catcgggaat gctgtgcatt aagaggaaat
     1261 ggctggggaa agaaacatgc tactgtggat tatgtttttc actattctcc ttatagatta
     1321 ttccaatatc atcagcttgt catggatgaa atggaaaaac gtggttacag cccagcacca
     1381 gagtggaaaa atcctatgta tcgtggcaaa tcatgcgaag cattttttga tttacctact
     1441 gtagagcgca acgtgccgat ttatccggaa cacgatgaag tttatctagc agaatgttta
     1501 gcaaatttgg agcaaaaagg aatttatctt acctaatata actagaaaag ccttctatct
     1561 cctcttactt cattaaaaag ctttaaccat cttagatcaa gctaaaatgt cgtaagtttt
     1621 agggttatct tcatcctcac gtgcaaaact aaaagcgtac tctagttttg gttcatcgcc
     1681 aacttcccta gacataaatg aacatctcct gacgtggttt aaaagtaccg catcttcgac
     1741 gtctgtatac gatgcacagc acagattgct tcttttgatc tttcttttga tttttctgtt
     1801 gcttcttcta caccttcttc gattactaca tcgatgctta caattgcttc tgcctaatat
     1861 tcccatgcat cgtcgattta agcctctgta gtcactgtga tcgtttccac attttaactc
     1921 ctctgtcgtg gcttcagttg attttgtgat cagttcagtt gctaagccag agtcagaaca
     1981 acctgccatt gctagcgtta atat
//
LOCUS       DAKBVG010000278         1987 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000278 DAKBVG010000000
VERSION     DAKBVG010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.281"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            <1..>1987
                     /locus_tag="OUD09_002605"
     CDS             <1..>1987
                     /locus_tag="OUD09_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HCT9693826.1"
                     /translation="DYPKKYIEKLNQDISSYKEVAKITAEMTQTFDGISRLIMLDRYA
                     FKDMSGKTLTVGDLVILTTHADPQYPARGIGKIEEINKEQLIIRIEEEFQSSLTDPEE
                     IASGRVIRNINEVEKPLELYFEQIAARVARGISDIEGEKKAQFFTEFYQHLTEQNLIP
                     AGRVLYGAGTNSEVTYFNCYVMPFIHDSRGGLAKHRQEVMEIMSRGGGVGTNGSTLRP
                     KSAIVHGVNGKSSGSVSWLNDLANLTNLVEQGGSRRGAQMIMLNDWHPDIFEFIISKI
                     QNPAILQNLIHTSQSPTVRQLASEKLTFEPLTANEKEWLEFLVDHPTNDMEDKFLKTA
                     QQKLKAGGTYSVKDPDFLTGANISVCLTKEFMEAVETDQTYELRFPAIEDYDASEMAD
                     YDQHWMEVGDVREWAETGRKVKIHQTIKARELWDLITYCATYSAEPGIFFIDNANEMT
                     NAQAYGQKVVATNPCGEQPLTPYAVCNLAAINLANMVDKKTKVVDFEKLTDVVRASVR
                     FQDNVIDATPYFFKKNEQQAKGERRVGLGVMGLADLLIYCEKVYGSEDGNRLVDQVFE
                     KIAITAYQTSVELAKEKGSFPFLVGKTVEETTALRQRFIESGYMKKMPESIRQAILKY
                     GIRNSHLLTVAPTGSTGTMAGVSTGLEPYFSFSYFRSG"
BASE COUNT          661 a          334 c          443 g          549 t
ORIGIN      
        1 gattatccta aaaaatatat agaaaaatta aatcaagata tctcttctta taaagaagtc
       61 gctaagataa ctgcagaaat gactcaaaca tttgatggga tttcaagatt aatcatgctt
      121 gatcgctatg catttaaaga tatgagcgga aaaactttga cagtcggaga tttggttatt
      181 ttaacaaccc atgctgatcc acaatatcca gcaagaggga tcggaaaaat tgaagaaatc
      241 aataaggaac agttgataat tcgaattgag gaagaatttc aaagctcgtt aactgatcca
      301 gaagaaattg cttcaggacg agtgattcga aatataaatg aggttgagaa accgcttgaa
      361 ctttattttg aacaaattgc agctcgtgtt gctagaggaa tctctgatat tgaaggagaa
      421 aaaaaagctc agttttttac cgagttttat cagcatttga cagagcaaaa tctcattcct
      481 gctggtcgtg tactttatgg tgcaggaacg aattcagaag taacatactt taattgctac
      541 gttatgccat tcatccatga ctctcgtggg ggcctagcaa aacatcgtca agaagtgatg
      601 gagattatga gtcgcggtgg cggtgtagga acgaacggtt caacgctacg gccaaaatcg
      661 gcgatcgtcc atggggtgaa tgggaaatca tcaggttcag tttcgtggct caatgactta
      721 gctaatttga cgaatttagt tgaacaaggc gggagtcgtc ggggagcaca aatgatcatg
      781 ttgaatgatt ggcatccaga tatttttgaa ttcattatct ctaagattca gaatccggcc
      841 attttacaaa atttgatcca cactagccaa agtccgacag tgcgccaatt agctagtgaa
      901 aaactgactt ttgaaccact gacagcaaat gaaaaagaat ggttagaatt tttggttgat
      961 catccgacaa atgatatgga ggataaattt ttaaaaacgg ctcaacagaa attaaaagcg
     1021 ggtggtacgt acagtgtgaa agaccctgat ttcttgaccg gagccaatat ctccgtctgt
     1081 ttaacaaaag aatttatgga agctgtcgaa accgaccaaa cttatgagtt acgctttcca
     1141 gcaatcgaag actatgacgc tagtgaaatg gctgattatg atcaacattg gatggaagtt
     1201 ggagatgtca gagaatgggc tgaaacaggg cgtaaagtaa aaatacatca aacgatcaag
     1261 gctcgcgaat tgtgggattt gattacttat tgtgcaacgt attcagcaga accgggaata
     1321 ttttttatcg ataatgctaa tgagatgacc aatgctcaag cctacggaca aaaggtagtt
     1381 gcaaccaatc catgtgggga acagccattg acgccttatg cggtatgcaa tctagcagca
     1441 attaatttag ccaatatggt tgataaaaaa acgaaagttg tcgactttga aaaattaact
     1501 gatgtcgtaa gagctagtgt tcgttttcag gacaatgtaa ttgatgcgac gccttatttt
     1561 tttaaaaaaa atgagcagca agctaaaggt gaacgacgcg ttggacttgg ggtcatggga
     1621 ttggcagatt tattgattta ttgtgaaaaa gtctatggat ctgaagatgg aaaccgttta
     1681 gtagatcaag tatttgaaaa aattgctatt acggcttatc aaacgtccgt cgaactagca
     1741 aaagaaaaag gaagcttccc atttttagta ggcaaaacag tcgaggaaac aaccgcgctt
     1801 cgtcaacgtt ttattgaatc gggttatatg aagaagatgc cggaatcaat ccgacaagcg
     1861 attctaaaat acggtattcg taattctcat ttactaacag tcgcaccaac agggtcaacg
     1921 ggtactatgg ctggtgtttc aaccggacta gaaccatatt tctctttttc ttattttaga
     1981 agtggtc
//
LOCUS       DAKBVG010000279         1981 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000279 DAKBVG010000000
VERSION     DAKBVG010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.282"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(45..1232)
                     /gene="tuf"
                     /locus_tag="OUD09_002606"
     CDS             complement(45..1232)
                     /gene="tuf"
                     /locus_tag="OUD09_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCT9693827.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            1600..1956
                     /locus_tag="OUD09_002607"
     CDS             1600..1956
                     /locus_tag="OUD09_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCT9693828.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT          586 a          410 c          365 g          620 t
ORIGIN      
        1 tattttaaac atttttcatt accaaaatat tcgagtcgcg tttcttatgc ttcgatctct
       61 gtaacgatac ctgaacccac tgtccgtccg ccttcacgaa ttgagaatgt cgttccgttt
      121 tcaacagcga ttggatggat caagtcaaca tcaatcgtca cgttgtcacc aggcatgacc
      181 atttctgtat cttctggcaa tgtgattgtt cctgtaacat cagttgtgcg gaagtagaat
      241 tgtggacgat agttgttgaa gaatggagta tgacgtccac cttcttcttt tgtcaacacg
      301 tacacttctg ctttgaattt tgtatgtgga gtaatggaac ctggtttagc aagcacttgt
      361 ccacgttcga tatcgtcacg ttggatcccc cgtaacaaca cgcctacgtt atccccagct
      421 tccccgtaat ctaacgtttt acggaacatt tctacacctg ttacaactgc tttttgtgtt
      481 tcaggtttga tccctacgat ctctacctca tcaccgacac gaacagcacc acgatcaata
      541 cgacctgagg caacagttcc tcgacctgta attgagaaga cgtcttccac tggcaatagt
      601 aatggtttat ctgtatcacg ttctggtgtt gggatatatt catctactgt atccatcaat
      661 tccataatag cagcttcagc atctggatcg ccttgcaatg cttttaatgc ggaacctttg
      721 atcacaggag tgtcatcgcc tgggaaacca tattcgctca ataactcgcg aacttccatt
      781 tctaccaaat cgatcaattc ttcatcatcg actaaatcaa ctttgttcaa gaaaacaatc
      841 aggtatttta caccaacttg gcgtgacaat aaaatgtgtt cacgtgtttg aggcatcgga
      901 ccgtcagttg cagaaacaac taagattgca ccatccattt gtgctgcccc tgtaatcatg
      961 tttttcacat agtccgcgtg tcctggtgca tcaatatgcg cgtagtggcg tttttctgtt
     1021 tcatattcta cgtgtgcggt attgatcgta atcccacgtt cacgttcttc tggagctgca
     1081 tcaatactag catagtcttg aggatttgct aggccttttt tgcctaatac tgtagtgatc
     1141 gcagcagtta aagtagtttt cccatggtca acgtgtccaa tcgtaccaat gttaacgtgt
     1201 ggtttacttc tgtcataatg ttcttttgcc ataatttaca aacctcctaa ataatataga
     1261 tcactgaaaa atttcagctc tcaccttttt attatagtct ttttttgact aaatcaaaaa
     1321 gaatacgtcc gacttacttt ttgtaagatt cgaactaagc ttattatacg tcaatattgg
     1381 tcaaagtcaa ctaagagcac tcgcttgtct tatacgcgaa aaaagtacct tttataaata
     1441 aaaaaatgtg ttttttgaga aaaacttcaa gatagtcctt aactttcttt tgaagcaata
     1501 agaacaaaat gaggaaaagt ttaaaattca gctgttgcca acaactttca atagacgaaa
     1561 tcttctattt cgttaataat aaggaaagag gtgaagtaca tgaaaaaatt aattggcgta
     1621 cttgtcgttt taggcatatt tctcggcgga agttttgcag cttatcatta tttttacggc
     1681 ggcgaagctt attatacaaa aatcacgacg aatggcgaaa tgtcagctac ccaagcagat
     1741 aacggagaga aattcacgac ttatacctac aagcaagcgg cttataataa agatggtgaa
     1801 aaaaaacagg tgacgcttcg cgaagaaaga gaacatccat taaagatgaa tgcgtatctt
     1861 aaactcaaag tcaatcctcg taaaggtgta ttaagctggg aagaaataaa ggcttctgaa
     1921 gtgcctaaaa atgcggcaga aaaaatcgat aattagtaaa tggtcttaaa attttattag
     1981 a
//
LOCUS       DAKBVG010000280         1980 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000280 DAKBVG010000000
VERSION     DAKBVG010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.283"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(<1..424)
                     /locus_tag="OUD09_002608"
     CDS             complement(<1..424)
                     /locus_tag="OUD09_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HCT9693829.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQI"
     gene            complement(533..1564)
                     /locus_tag="OUD09_002609"
     CDS             complement(533..1564)
                     /locus_tag="OUD09_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCT9693830.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT          610 a          327 c          340 g          703 t
ORIGIN      
        1 gaatctgttt tgattttgtt ccatttgaaa tttctctcac tcgctttttt gtctcatcat
       61 caaataaaat gaaagggtct tctaaacgat cgataaatac ttccattgtt gttgttggta
      121 gatgataaga agtcatgaca ggcactgcac caaggtaaga cacagaaaca gctagaaggt
      181 atgtgtcaaa agcggaactc ttataaataa taactttgtc tccggatttc actccaagat
      241 gagccaattg ataagcccgt ttcaacacac tttcgcagca ttttttgtaa gttgtttcaa
      301 gtcctaattc tgggaaagct gaaagtggct catcaaagat aatcggggtc tcggaaaagt
      361 tagctgcagc ttcttgaaaa ttcgtaaata agttcaaagg ggaatacgtt atggtctcca
      421 tcattcacac tccattatct tttgttaagt aatatacaaa ttcaagataa tcgtaacata
      481 atcctttata taataacata gtttattgtg aaaaaaagat catacatttg ttttatatgg
      541 aagaaacccg aacgaatgcc gctaattcgt gataagaaaa atcaaccatc ttttcccaga
      601 tatcttgaaa agaatgggat gagaggtatc gattaccaca taaataattt accgcgtcag
      661 catcatctaa atatagttct gcataataac gatatgtttt tctgcatagt tctggcgata
      721 cgtttttata tactgtaatt ttgctcaaaa tttggcgaat agtccgagga gaaatttgct
      781 gatcatgtga ggaacgaaaa acaaaagaat tttcgtccgt attttgtgct gttaacttaa
      841 aatagttctc cagaaatggc atcgcttctt ttggtattgg tactttattc agtctgtttt
      901 cttttctatt cagattaaga atctgcttgg tcaaagatac atctttacgt ttaagttcta
      961 ctacttcatg gatctttaat ccgcaataca gaagaatggc agtaattgca aggttcctcg
     1021 gtctattctt ttcttcaaag ctagcttctc tgcttgtttg aaagccttca atcgggataa
     1081 acaaatgctc aaaatctttg aaaagttctg catcgatata tcgcggagct gctttggttg
     1141 tttccggtaa acttgtaccc aaaagagaaa tctcttcttc aatcagtcct ttttgataca
     1201 aaaatcgtaa aaaatgattt aatgaatatt gcttccttga aatcccgtaa tagctatttt
     1261 caaacaattg ctccgtttct tggttcgctg tagtttggaa cgaacgagta tgtcgataga
     1321 ttttagctaa gaaattctga taatcttgtt tttctaaatg gataatcagc tgatcatcca
     1381 cttcttcaga aaatactttt ctcaaataat cagtaaacag atgaaaatct tttgcgtatt
     1441 catagcgact gcctaatgaa agggaagaac gattttcttc taaaaactgt tgaaagacgg
     1501 aagggagcaa gtgtgtacgt tgctttaact tttcttgcag ttgttttcta gttttgctgt
     1561 ccattacaaa ttccctccaa tagtgttcat tttctatatt ttaccgcatt tcatcaaatg
     1621 aagatagaca gtttgtcttg atcttaggaa gcaaattttt aactaagtac tagctaaaag
     1681 atatgaagta agaagataca tttttagtta ttttattagt gcagtcattc ttttgatctt
     1741 ttaattgatt tatcctcttt ttagctatgt ggttacttca tgagcaatga aatggatttg
     1801 gttcgttcta atggcaggag gctctttctt ttatttccga aaaattagaa aacatttttt
     1861 cagaatcgat tggctctcca tttattcaat aatggtttgt ctaacgatat tgttgggata
     1921 attcttaaag aagtattaga aatagttggt acttcattac tttacatgaa gatttattct
//
LOCUS       DAKBVG010000281         1976 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000281 DAKBVG010000000
VERSION     DAKBVG010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.284"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            137..826
                     /locus_tag="OUD09_002610"
     CDS             137..826
                     /locus_tag="OUD09_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCT9693831.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            840..>1976
                     /locus_tag="OUD09_002611"
     CDS             840..>1976
                     /locus_tag="OUD09_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCT9693832.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIA"
BASE COUNT          615 a          275 c          397 g          689 t
ORIGIN      
        1 tttctccatt atactccaaa ataataaaaa aaactcctaa atactccatt tttatcttta
       61 cttttatgaa agcggatgct atattgtttt tggagttgtt atccaaaaat aataaaaaat
      121 tggagtgttt aggataatgg ataaaaagag gtttttagat cttatttcaa aaggtttgat
      181 tgtttcctgt caagcattac ctggtgaacc attttacaaa gaggaagaaa gtttaatgcc
      241 ttattttgct ttggctgctc aaagggctgg agcagttggt atacgtgcaa attctgttcg
      301 agatattatt gaaattagaa agtgtgtatc attaccaatg attggaatta ttaaaagaga
      361 ttatccacca gaatctccat tcattactgc cactatgaga gaagtagatg aattagtaga
      421 aaccggtgtt gaggtaatcg ctcttgattg cactcttaga aatcgacatg atggactatc
      481 tataaattct tttattacta aaataaaaga aaaatatcct catcagttat ttatggcaga
      541 tatttctaca tttgaagaag gcaaaaatgc atataaagca ggaatagatt ttgtaggaac
      601 tactttaagt ggatatactg aggaaagtaa aaaacaagat gggccagata tagatttgat
      661 taaaaaattg gtagaagaag ggatttgtgt aattgctgag ggaaggattc atactccaga
      721 acaagcaaaa gagataaaaa aaataggagt tgcaggtatt gttgtgggtg gtgctattac
      781 tcgaccacag gaaattgcat caagatttgt taaagcatta agttaggggg agaaataaaa
      841 tgtttaaaaa attttcacaa ttaggacgcg ctttcatgtt accaattgct atattaccag
      901 tagccggatt actattggga ctgggaggag cgttaacaaa tgagagtgct ataaatgctt
      961 atccattttt agatcaaccg tggttgcata caattttaag tattatgagt tatgctggaa
     1021 atgcagtttt tgcaaaccta gcactaattt ttgcaattgg tattgctgta ggacttgcaa
     1081 atggagataa aggaacagct ggtttagctg gtggagtagc ttatctagta tatactgcaa
     1141 ctattagtgg gtttcttgaa cttttttcag caaaagatgc aacactagat acaggtgtag
     1201 tgggatcaat tgttgttggt ggtactgttg catttttaca caatcgttat agaaaaattg
     1261 aattaccaca attcttaggt ttttttggag gatcaagatt tattccgatt gtttctagtt
     1321 tagctgcaat cattattggt tctttctttt atttaatttg gccaccaatt caaaatggtt
     1381 tagtagttgc tggtgaacaa attgcccaaa tgggtagttt aggaactttt ttatatggtt
     1441 ttttattgcg tttaacaggt gctgttggac tacaccacac catttaccct ttgttctggt
     1501 atacatcttt aggtggtacc gaaactgtag ctggatcaac aattgctgga gcccaaaata
     1561 ttttctttgc ccaactagct gatccaaacc acactggtct gtttacttat ggtactagat
     1621 tttttgcagg aagatttgca acaatgatgt ttggattacc agcagcatgt tatgcaatgt
     1681 atcgtgcaat tcctaagaaa aatcgtaaaa agaatggtgg gctttatttt agcggggccc
     1741 ttacatcatt tttaactgga attactgaac cagttgaata tatgttttta tttgttgctc
     1801 cttggcttta tgtgattcat gcattcttag atggattatc tttttatttt gctgatatct
     1861 taaatattcg tgtagggaat tccttttcag gtggtttgat cgacttttta ttattcggtg
     1921 tacttcaagg gaatgacaaa actaattggt taaaattaat tccttttggt attgca
//
LOCUS       DAKBVG010000282         1959 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.287, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000282 DAKBVG010000000
VERSION     DAKBVG010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.287"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            273..839
                     /gene="amaP"
                     /locus_tag="OUD09_002612"
     CDS             273..839
                     /gene="amaP"
                     /locus_tag="OUD09_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HCT9693833.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            852..1040
                     /locus_tag="OUD09_002613"
     CDS             852..1040
                     /locus_tag="OUD09_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HCT9693834.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1053..1610
                     /locus_tag="OUD09_002614"
     CDS             1053..1610
                     /locus_tag="OUD09_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCT9693835.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1642..1884
                     /locus_tag="OUD09_002615"
     CDS             1642..1884
                     /locus_tag="OUD09_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCT9693836.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          715 a          305 c          404 g          535 t
ORIGIN      
        1 aataaataga atcttgtaat aataactgag cataatcttt gcaattttta atcttgaata
       61 atatccttta agtatagcca gcaatcaaaa gtaaatagag aataagtaag caattgttat
      121 ttcagtatca gactggcaat aaaacgccgt tatgatccag caatgtttgg tagcgcttta
      181 tttactgatg ctatgctaac tgttcgctat ctatactgtg attgtcggta gagttattta
      241 cagggtaaaa ataagtttat aggaggtgga aaatgaataa ggggttaaaa ataatcggtg
      301 ttatcatgag tttactgcta ctttcagtat ttctagcgat tgcgttgatc aatgcgccgt
      361 atattatgcc tgaacaattg gaacgatttc gcttctttac tttaacgaat tactacatgc
      421 aacaatatat tttctgggca gctgtagtat ttgccgttct tgctattatc atcttgctag
      481 tcgttctttt ctatccaaag tcaagaggaa ctttcgttat gaaacgggaa gatggcaaac
      541 taacaatcga caaaaaagcg atcgaagggt tggtacgttc ccatttacac gaagaagagt
      601 tcattcattc acctaaagta cggatacgtt caacgaaaaa tcgaatccat atccatgtca
      661 acggagactt gaaacgaact tcttcattag tagggaaaac aggtgtattg atgcaagata
      721 tcgaagaaga agtaactaaa gtactcggaa caaaagaaac ggtcaaagtg gcagtcacat
      781 acagcggtta tgaagaacaa gaagatacaa gagactacaa gcactctcga gtggaatagg
      841 agggccaaat aatgaaagaa ctattttcag catacaagct ccctattatc tttgggggtc
      901 ttggactact gctagctgtt ttatggttaa cgattggatt tttcaagaca ttgttgatca
      961 taatcatgac agcaattggc gcaggaatcg gtttttattt gaaacagtca ggaatagttg
     1021 atcaattttt taaatcatag gaggaaacaa agatggaaaa caaaaacatg aatacaacaa
     1081 atggatcagt tttggcaaat gaaaaaggag aaaaccgcat gatgaatggt gaattgactt
     1141 atgaagacaa agtcatccaa aaaatcatcg gttatgcatt gaaagatgta gatggtctat
     1201 tgacattaga tggcggattt ttctcaaata tggctgaaaa attagtcaac acagataacg
     1261 taacagcagg tatcaataca gaagtaggta agaaacaagt agctgtggat ctagatgtga
     1321 tcgtagaata cggtaaagac atcgaagata tttacaacca aatcaaagaa ttgatcagta
     1381 ctgaagtaaa caatatgaca catttagatg ttatcgaagt gaatgtgaat gttgcagaca
     1441 tcaaaacaca agaagaatac caaaaagatc aagaaacagt acaagacaaa gtaacagaag
     1501 cagcaaaatc cacaggacaa tttgcttcac gccaaacaga taaagcaaaa tctgctgtag
     1561 gcaaaggtgc acaaaaagtc aaagaaaata acgaaccacg tgtacaataa gaagtaagaa
     1621 gtcagttagg aggaatttaa aatgggattt atttgggcat taatcgtcgg cggtatcatt
     1681 ggagcaattg caggagcaat cacgaataaa ggatcatcaa tggggatcat cgcaaacatc
     1741 attgctggtt tagtaggttc agcaattgga caagctttac taggaacttg gggaccaaca
     1801 attgcaggaa tggctatcat tccatcattg atcggtgcaa tcattttagt attagtcgtt
     1861 tcattctttg cacgtaaatt ataagcaaat tgaagaaaaa cttatgttga aatgaagaac
     1921 aaatttgaga accgtagcga cactcgctgc ggttctttt
//
LOCUS       DAKBVG010000283         1956 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.288, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000283 DAKBVG010000000
VERSION     DAKBVG010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.288"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            724..1197
                     /locus_tag="OUD09_002616"
     CDS             724..1197
                     /locus_tag="OUD09_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HCT9693837.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1206..1433
                     /locus_tag="OUD09_002617"
     CDS             1206..1433
                     /locus_tag="OUD09_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCT9693838.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          632 a          320 c          292 g          712 t
ORIGIN      
        1 gaattcagtt ggactactac attatgggaa tctctattct ttttcatctt cgtatttttg
       61 ttacaaaatt atcgaaaaaa ataaacatgt gccatctatc tgttggcaca tgttttagga
      121 tttatggtac tttttattcg gcaatccatt ttatttttct acttcttctt aatacctcta
      181 acacttttga tttataaact aatccaatgc atctacttgc gcttattaag ctatttcagc
      241 tagatagtac caccccatgc gtcaatgctt gattttcctt ttgtataaca ttgtataagc
      301 aataaaggac aaataagtgg tgattacacc aaaccattga ataagaaaaa ttctgatacg
      361 taaataaaca tgaacttgaa aaaattaatg aatcggttag ctactacgta gttgtctttt
      421 ttctcataca gttctgttaa cttacacttc tcttcgctag ataataaaaa atcctccaac
      481 tgtattgtcc aatataatcc caggcatcta aactagcatt atagaagaga cacacataga
      541 atctaaaata actggtttta aatgtttctt acaatcaaaa aaagctgtta aatgcactcc
      601 acaccttcat actctatcat tttttataaa cgatatattt ttattgtttt tcgataaaaa
      661 atagttgttt tacaataatt attctgctat attttagcta tcaaagataa aagagggata
      721 aaaatgaaat ttaaaacatt gctattaaaa tttgtcgttt taggtataag tgccgtattt
      781 attttgttcg gcgtactttt ttatactcag atcgctacaa gcgaaaaagg attcaatttt
      841 gattggcaga ccacattatt tgtttctgtc atttactttg cccttctcct agggatcgtt
      901 attgcttatt tcttattgaa actattgaaa aaaatcgaag ataaccaagc tttttccact
      961 caaagcttaa acatactaaa aaaaatcgaa cgaaccattt tgtttattgg tttcgccttt
     1021 tttggcttac ttcctaaatt tatgaaagta gcggatttag acgatgctcc aggtttgatg
     1081 ttggtaggaa tcgctgtcgt attcattcct tttgcagtat acacattaac aagcgtgctg
     1141 gaaaaactgc ttgaacatgc gattttactt aagacagatc atgatttgac agtgtgaggt
     1201 gcgacatggc aattatcgtt aatttagatg tagtattagc tcaaaaaaaa atgagtgtga
     1261 ctgaattgag tgagaaagtg ggcattacga tggcaaacat ctctattcta aaaaatggaa
     1321 aagcaaaagc catccgtttt tccactctcg aaaaaatctg ccaagttttg gagtgtcaac
     1381 caggtgattt gttgacctat aaaaatgact cgaatactat accagaagaa taaaataaag
     1441 tagaacaaac agtaaataaa aaataatctg aatgatagat gtgtgagact tttagaagtt
     1501 atttacgtct attattcaga ttttttattt cgattgaata ccgttttatc tataaataag
     1561 atatgtactg actcatacaa agggggcctt ttcttctcca tgtactatca cttttcctga
     1621 tacaactggt ttcctgttac gttgtagttg gatctgcttt tagtataata ctttattcat
     1681 tccaccagac tgatagcagt ttgagcagtc taaataatca tgtagtatta caggccattc
     1741 tgtttcatac cttgaagtgc ttaaaacttc agctaatggc ccattgtgtt aactcttact
     1801 ccattctttt ttagtaagct cccatatata ctgtaaagcc ttctatatat tatgtcatgt
     1861 tgctttttaa catttactat gtagttttga taaaatgatt tgaataacag tgatggacaa
     1921 ttctttttta tttttaacat tattttgata ataatg
//
LOCUS       DAKBVG010000284         1934 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.291, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000284 DAKBVG010000000
VERSION     DAKBVG010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.291"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            <1..623
                     /locus_tag="OUD09_002618"
     CDS             <1..623
                     /locus_tag="OUD09_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCT9693839.1"
                     /translation="NNLLSKYKNSYVRNMYGIFQMSLDRAVVLGLLEKNPAKVIGNVK
                     KRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVMRLGEATALTWKDIDF
                     ENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFLKAWKERQVSIGGMSF
                     VLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLLISQ"
     gene            complement(907..>1934)
                     /gene="xerS"
                     /locus_tag="OUD09_002619"
     CDS             complement(907..>1934)
                     /gene="xerS"
                     /locus_tag="OUD09_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HCT9693840.1"
                     /translation="IKDFVQDREDKDQSPRTTLEYLKNYKLFYEWLLSESIVPDTSIT
                     SIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNNNLGLSTSTINRNITALKVLF
                     KYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARANAIEKKLFLDKDTQNYLDYIA
                     NEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSEVININLDDLSLDKNNVVVTR
                     KGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKALFLAIYQGEAKRISGIAIERM
                     VAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQLGHSSTNTTTLYTHIDNAAT
                     IDALNAL"
BASE COUNT          621 a          299 c          331 g          683 t
ORIGIN      
        1 aaaataattt attgagcaaa tataaaaatt cttatgtaag aaacatgtat gggatttttc
       61 agatgtcatt agatcgagca gttgttttgg gactacttga aaaaaatcct gcaaaagtta
      121 ttggaaatgt gaagaaaagg aaaactacag ttgatttttg gacaaaagaa gaatttacaa
      181 aagttatttc aactttttat attgatgatt tgtaccaaca ttttggtttt atttgtatgt
      241 ggttactttt tatgaccgtt atgcgtttgg gagaagccac tgcattaact tggaaagata
      301 ttgattttga aaataaaaac ttgcgtgtga ataaaacact ctattataag agagccaaaa
      361 cctatgagtt tgtagaaccc gaaacggctt ctagtattcg tacaatcgaa ctagacgaca
      421 atacaattac ctttttaaaa gcttggaagg agcgtcaagt ttcaatagga ggcatgtcgt
      481 ttgtactttc ttataatgga acacctactc agaaatatac aatccgtcat attatgagaa
      541 gacatgcaac gatcgttggt gttcatataa tccgcataca cggattaagg catagtcatg
      601 cttctttgct tatatcacaa taaaaagatg cacttcgaat taaagaaagg ttaaaacatg
      661 ctgatgtaca aacgacttta ggaacttatg gtcatttgta tctaaattca ttgagagaga
      721 taacaaatga tcccacagat tatatccaaa ctaatttttc taaaatcaaa ccgacaccta
      781 tttacgataa taaaaaataa atgataaaca aaagtaggat cacaccaaaa gaaacacact
      841 agaaaaacta taatacaggc ttttctagcg tgttgtttct tatttccgcc tatttataca
      901 ttatactcat aaagcattta atgcatcgat cgtagccgca ttgtctatat gggtatataa
      961 agttgttgta ttggtacttg aatgtcccaa ttgttgggca gttaatacca atgaatttgt
     1021 ctgttggtag agtctagtag ctaatgtatg acgcagtttg tgagggctga cttgaattct
     1081 aaagcctttt gaatattttg ctaccatccg ttcgattgca ataccggata tacgcttggc
     1141 ttctccttgg taaattgcta gaaataaagc tttttcgttt tctgttactt tatatcgttc
     1201 tttccgaata gctagataat tagctaaata ttccattgcg aatgctgcta tattgacagc
     1261 atctctttta tttccttttc tagttactac cacattattc ttatctaaag ataggtcatc
     1321 taaattaata tttataacct cggagagtct caatccagat cctaaaatca gtgcattgat
     1381 cgccaggtct ctttctacat ctcgttgata atagatcaaa gctcgtttac tcaaagtatt
     1441 tttatattca ttggcaatat aatctagata attttgtgta tccttatcta gaaataattt
     1501 tttctcaatg gcattggcac gtgcagctaa cgtagtttta tctgtgatgg ttgctacttg
     1561 atccatagga ttattgtcta agtatggttt tcctagagga ttattggaac ttttcgaaag
     1621 atatttaaat agaaccttta atgccgtgat atttctattg atagtagaag tagatagacc
     1681 taagttatta ttatctttga gcttagtttt tgttgtctct ttttttacat tttcttttgt
     1741 gcgacgtttc aaatgagatt tgtacaagtt taggtcatag gcagttaact gtcttatcga
     1801 ggtgatagaa gtatcaggaa cgatagactc actcagaagc cattcataaa agagcttata
     1861 attttttaga tattctaaag tggttcgagg tgattgatct ttatcctcac gatcttgaac
     1921 aaaatctttt atat
//
LOCUS       DAKBVG010000285         1899 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.292, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000285 DAKBVG010000000
VERSION     DAKBVG010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.292"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(144..1868)
                     /locus_tag="OUD09_002620"
     CDS             complement(144..1868)
                     /locus_tag="OUD09_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCT9693841.1"
                     /translation="MKKKLLTLFLVMSFSIVLSVYYNTIVFSEQINYTEEQYNIEKEK
                     ALKVGYSEEQFKQIMEIPTNLSNENSETRIVNYTMTSNQTKVINKAMEQIGKPYEWGA
                     SGPNSFDCGGLVKYVYKQAVNIELPMGTTNQEQYGTEVSLNSLKPGDLLFYGNRGATY
                     HVGIYKGNGVMIHAPQPGETVKEVNIQYFYPNFAKRILPDEPDYPYIDYNKMVTVTKA
                     WSIWNDLQFSHEIKKAIIGDNYKIGKVYTNPENNNKYGEILVSNKVYGYINFYAVKEL
                     TSVQINRYLTSKDSGQPTWGNLECTISKGQTTKDKIYFVKGAYNLGDGKYLYSIYKDQ
                     DSSEWLGYLKAHVSLAYTPIEEINKNVTVTKNWSIWNNLQWEKEIEKPQIGSVFSARL
                     KLTNVSNNAVYYKLYKSGKFYGYINAEAVKDLTTTKLNKYVTFSVNNEDFWSSLDVNY
                     SKGKTERGRVFYISISYNTADNQPIYSVYTDETCTEWRGYYKGNNFEDTQITMLENKS
                     VKVTKSGYTVWGDLNFWTKSGISNTGDIYTTDRKFYNFTNNAYYYELKKDGRVYGYIN
                     SEAAVEMN"
BASE COUNT          587 a          343 c          231 g          738 t
ORIGIN      
        1 tttacttaaa taatttttgg taattaaact attatattac aaaacaagaa aaactattga
       61 ttaattcttc taccctcgct tcagaaaaat gattaataat ataaaaatat tgctgcccca
      121 taagattaag ggacagcaat attttagttc atctctacag ctgcttcact atttatatag
      181 ccatagaccc tcccatcttt tttcaattca taatagtaag cattgttggt aaaattataa
      241 aattttcggt cagtggtgta tatatctcct gtgttcgata ttcctgactt agtccaaaaa
      301 ttcaagtctc cccatactgt atacccactt tttgttactt tcacgctttt attctcaagc
      361 atagtaattt gagtgtcttc aaagttgttt cccttataat accctcgcca ttccgtacat
      421 gtttcatctg tatatactga ataaatcggt tggttatctg cggtattata tgaaatacta
      481 atgtaaaata ctcggcctct ttcagttttt ccttttgaat aatttacatc taaagaagac
      541 caaaaatctt cattattaac actaaatgta acatacttat taagctttgt tgttgtaagg
      601 tctttgacag cctcagcatt gatataacca taaaattttc cagatttata aagcttgtaa
      661 tagacagcat tattcgatac attggttaac tttaatctag ctgaaaatac agaaccaatc
      721 tgaggctttt caatttcctt ttcccattgt agattattcc aaatcgacca atttttagta
      781 acagttacgt ttttattaat ttcttcaata ggagtatagg ctaaactaac atgtgctttt
      841 aaatacccta accattcaga ggaatcctga tctttataaa tagaatatag atactttcca
      901 tcccctaaat tatatgctcc tttaacaaaa tatattttat cttttgtagt ttgtccttta
      961 cttatagtac actctagatt tccccaagtt ggttgaccac tatctttaga agttaaatat
     1021 ctgtttattt gaacagaggt caactcttta acagcataaa aattaatgta tccatatact
     1081 ttattactta ctaatatttc tccatattta ttattatttt ctggattagt ataaaccttc
     1141 cctatcttgt aattatctcc aataatagct ttcttaattt catgactaaa ttgtaaatca
     1201 ttccatattg accaagcttt tgtaacagtt accattttat tatagtcaat ataagggtag
     1261 tcaggttcat caggcaaaat tctttttgca aagttaggat aaaaatattg tatgttaacc
     1321 tcttttacag tttctccagg ttgcggtgca tgaatcatta caccgttacc tttatatata
     1381 cctacatgat aagttgcacc tcgattgccg taaaataata aatcaccagg cttaagtgaa
     1441 tttaaactta cctctgtacc gtattgctct tgattggtgg ttcccatagg cagttcaata
     1501 tttacagctt gtttgtaaac atatttcact aaaccaccac agtcaaaaga atttggtccc
     1561 gatgctcccc attcgtatgg ctttccaatt tgctccattg ctttattaat gactttggtt
     1621 tgattagaag tcatagtata atttactatt cttgtttcac tattttcatt tgataaattt
     1681 gtaggtattt ccataatctg cttaaactgt tcttcactat aaccaacttt taaagctttt
     1741 tctttttcta tattatactg ttcttctgtg tagtttattt gttctgaaaa aacaatagta
     1801 ttgtagtaca cactcaaaac gattgagaaa gacatcacaa gaaataaagt taatagtttt
     1861 ttcttcattt tttacctctt ctccttattt tttacaagc
//
LOCUS       DAKBVG010000286         1880 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.293, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000286 DAKBVG010000000
VERSION     DAKBVG010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.293"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(261..875)
                     /locus_tag="OUD09_002621"
     CDS             complement(261..875)
                     /locus_tag="OUD09_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCT9693842.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1481..1858)
                     /locus_tag="OUD09_002622"
     CDS             complement(1481..1858)
                     /locus_tag="OUD09_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693843.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
BASE COUNT          622 a          379 c          288 g          591 t
ORIGIN      
        1 ttgtctcttt ttccactact ttttattttt ctgacactac atattcttat aactcatttc
       61 tcataactgt gaaacaactt ataaaaataa caaactttaa acaaaacaag cttaaaatca
      121 aagatgatcc atcttaaaat aactaaaaca aaaaaagtag tcgttgttaa tcctcattaa
      181 taatcgtttt ctttttttca aacgctccat ttttatgtgg aacattattt ttttaaggaa
      241 ctttgtcaac atgtggataa ctattttcca cttgtgtcct ggactagctg ttttgctgta
      301 aaatacaaaa cttgctgcag caaaaagatc gttgcaagag aggctgttcc gcagatcgct
      361 ggatctagat ttggcaaagt caagactgca tcatgtaaat tggcgtggtt gtccaaaacc
      421 aaatcagcaa attcaaataa tcgcaaacca ctgctatgtt gcgatttgat acttcgtgac
      481 aaatcaaatc cagtaacaat gattaatgga tgaccatttt ctttgatcca ctgagctagt
      541 tcgatgattg ccgggtttct tccttcattc gaaagaagaa gaaaaatgtc ttctgatttg
      601 acagaaagct gatcaataat tgcctgtcct gcaccttcta gcgtttcata gacacctccg
      661 gctggatttt caatcaatat acttggtaca agatccgggg tttgatccca aaattcaaat
      721 gctacaccac ttagtgtgcg gctagcatat atctgcaatc taccaccttg attaatttgt
      781 ttcgcgatcc aagtgccagc ttgaagcatc agcggctctt ctatccttaa tttctcttcc
      841 aattggtgat aatagtcaaa ataacataaa ctcatttatc tctcccgcct agctatactt
      901 gacatgaata gaaacagctg cctaatctaa ggaagtacat aaaaaaacag tagttagaca
      961 gtaacacatg tggtctttgc tgtttcaaac acaaaaaagg cattgaacga atacgcccaa
     1021 taccttgaca tagcaaataa aacaacgtgc agaccatgga aaatatctac ataggggtag
     1081 aattttcacc ctagattagg tacaataaat taggtcgatg cactgcattg actatggcaa
     1141 aatgatcggt cagggtgtat gtgtgtgact ggtcattccc tgattcttag cgccaactaa
     1201 gggtcaggga gccttttttt attcagctgt ttctatctga atttattgta agtaatctct
     1261 tatccaagtg caagcaagaa aatacaaaaa aacattggta tgatttatat atatataaat
     1321 aacataaaaa aaggaatggc ttatcccgat acaacaagca ttcctttaaa aaagattctt
     1381 ttatataagt gaaaaaagct tttttccacc aattaaataa atctctcatc tcaatagttt
     1441 atccattatt ttatcttttt ttgcgtatac ctactaaata ttaccgtctt cttctcgttc
     1501 ttctactttg ttcgagagaa tctgtactag aaaaagtagt tgtatgtcta ccttttctgc
     1561 ctatccgaga ctttcttttc atatatggga tataaatgat tagatacagt acagcaaaaa
     1621 ataaagcaaa tacgcaagct tcccaactac ctaacgccaa ttcaaccatc aaggtagcaa
     1681 ccccaacgga tgctgcatac cagataaaaa tcagaaacgt ttcttttttt ctgaccatgc
     1741 gaaacatgat aaatagacta ataagcaata gtggcaacac aaatatccaa ttgcttcctt
     1801 ccatcagcca cttgagtata tcaagcagcg actgtgcaac agcgactaat tttcccatag
     1861 tgaacacaca ccttttttta
//
LOCUS       DAKBVG010000287         1856 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.294, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000287 DAKBVG010000000
VERSION     DAKBVG010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.294"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(538..828)
                     /locus_tag="OUD09_002623"
     CDS             complement(538..828)
                     /locus_tag="OUD09_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693844.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1036..1239)
                     /locus_tag="OUD09_002624"
     CDS             complement(1036..1239)
                     /locus_tag="OUD09_002624"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693845.1"
                     /translation="MNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLE
                     KAKDEVEDTADKSQQIVEEHLRK"
     gene            complement(1336..1728)
                     /locus_tag="OUD09_002625"
     CDS             complement(1336..1728)
                     /locus_tag="OUD09_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase"
                     /protein_id="HCT9693846.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEIN"
BASE COUNT          499 a          362 c          284 g          711 t
ORIGIN      
        1 ttaaggacct atggattgca gtaatgcaga attcgtcgaa taaactgcca aagacataca
       61 atcatattgc ttttaaaatt gacgaacaag atatagattc gtttgtctca aaaatccaaa
      121 tgttaggttt aactgtcgaa ccaggacgtt ctcgtgtgaa agacgaggct tcttcaattt
      181 acttttacga ctatgataat cacctcttcg agttacatac cggtactctc caggaacgtt
      241 taaaatccta caattccact acctgagact agtaatgact agtctctttt tatggcctct
      301 gatcccccca ttgagcgaat actaataggg cttcgaattg atctgttcaa tctttaagca
      361 atatccctca aatgagtttg gaaagtttag tgattcatca caaatactta aatacatgta
      421 gttatatata gattgaggtt cttgtgagcg tatgctcgtg tgggttcaaa tcccatcgtc
      481 tgaatccatc tttccaaaac aagcggctag gacatttttg tctcagccgc ttgttttcta
      541 gtttattctt tcatcttgtc ggaccttttt tcttgggtat agacttttct catattctac
      601 cacaattttg ggtttcccta acacatgata ggatctcaca acaaattgct ttctattatt
      661 ataataacct gtaacgacta acttcatccc ttcgtctact tcagctagga agttcaaact
      721 atgggaagca atcaaacaat tgaccccatt taaactgaaa cggatcaacg gtctggcact
      781 aacttgcaaa gtacggatct tatctactgt tccatatttt ttctccataa ccttcacctc
      841 aataccatta tacgaacata cattcccttt gtaaagaaga aactctgttt tatctttttg
      901 actttttgtc tattaccttt cttaaaatca tattggcact tcctgcgaag atgaaggaca
      961 tgtactaaaa aaaagaggca gcagtataga ttactgttgc ttctttacta atttaggttt
     1021 acttgttaag aaagattatt tacgcaaatg ttcttcaaca atttgctgag atttatcagc
     1081 tgtatcttcc acttcatctt ttgctttttc taacttttga atatattgtt tcaagcttga
     1141 gttttcagca gttgcttcgt ctaatttctt ttgtgtatct gttaactgag tttctacatt
     1201 tgttttttct tgtgtcagtg ttgcgatttg ttgattcaaa ccatttacag tattttgtaa
     1261 ttctgaaagt tttgcttgtg cttcttgtag tcttgctgtc caatctgcat tcagatgatt
     1321 gatttcatca tttttttagt tgatctcatc tagtttgaca ttgatttcat tttgcttgct
     1381 ttgtagttgt gcatccttgc tgactaattg actttctaag ttttgttttt ctgctgccga
     1441 ttgatttttt tggttttcta attcatttac ttttgtttgg tattcttgga catctgcctt
     1501 tgaatcttgc aatgtctgtt ctttttgtgc tagtcgagcg gtaagatctg taatcgcttg
     1561 ctctttatct gtcaatgtat gatcaagctg tgtcagattc tcattgattg tatcaatatc
     1621 tgtctctcca ccccacaaaa taatttcatt tgcgtaccgc gtgcctgtgt agccaattag
     1681 acatagaaat gcaacgacaa tcccaccagt aataaatttc tttttcattt gcatagctcc
     1741 ttaatccaat acttttacat actaaaagta agtgatctca aacacaatgt caagcgtttt
     1801 taaattacat atgtaatttt aatataaata ctatcaaata ctcatttaat cctcct
//
LOCUS       DAKBVG010000288         1828 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.295, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000288 DAKBVG010000000
VERSION     DAKBVG010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.295"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            <1..709
                     /locus_tag="OUD09_002626"
     CDS             <1..709
                     /locus_tag="OUD09_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCT9693847.1"
                     /translation="LVCLLITTFVFSFFTSYSSRFLKTNTLKRWLTALVVGLLSIIAM
                     YYVTDITARAWAHVPHITEVKKDTSDSSGTTSSNKVDLKRPDTDSGDVSNLRFELWKS
                     AIEIFKSKPISGGSPGYYIEYAHDKLPYTLMGKDTLTAHNFIFLVMAATGGLGLLTLL
                     TFLFIQCFKALRYCFTQPNLVKDKHFFAILMILTIAVSALFITELILVSTIGSFIFWG
                     YLGSIQKTISKEEGVTN"
     gene            843..1787
                     /locus_tag="OUD09_002627"
     CDS             843..1787
                     /locus_tag="OUD09_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCT9693848.1"
                     /translation="MDTILITGGAGFIGSTLANYLGKENKIVVVDDLSMGKKENLDMD
                     KQVTFIEGDVSDSQLMERIMKEYQFAYIFHLAAVASVADSVERPVETHRVNFDSALLL
                     LELARKYQSNLKRLVFSSSAAVYGDEPTLPKKEESVIRPLTPYAIDKFAAEQYVLDYC
                     HLYDVPTSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQLAGEKAEFTLFGDGSQSR
                     DFVYIEDVIQALLLVAKEEKALGQQFNVGTGKSTTLLELIHSIDQILGTELALKYEAE
                     RSGDIRDSLADISKIRSLGYQPKFDILSGMERYLKTEI"
BASE COUNT          620 a          265 c          347 g          596 t
ORIGIN      
        1 attagtttgt ttattaataa caacttttgt ttttagtttc tttacaagtt atagtagtcg
       61 ttttcttaaa actaatactt taaaaagatg gctaactgca ttagtagtag gattgctaag
      121 tataattgcg atgtattatg taacagatat aactgctcgt gcatgggcac atgttccgca
      181 tattacagaa gtaaaaaaag atacatctga ttcatcagga acaactagca gcaataaagt
      241 tgatttaaaa cgccctgaca ctgatagcgg ggatgtttct aatttaaggt ttgaactttg
      301 gaaaagtgca attgaaatat tcaagagtaa gccgatatcc ggaggttctc caggctacta
      361 tattgaatat gcacatgata aactaccata tacattaatg ggaaaagata ctttaactgc
      421 tcataatttt attttcttag tgatggctgc tacaggagga ttaggtctat tgacattgtt
      481 aaccttctta tttatccaat gctttaaagc cttgcgttat tgtttcacac aaccaaatct
      541 agtaaaagat aagcactttt ttgctatttt aatgattctt acaatagcag tttcggcatt
      601 atttatcaca gaactcattt tggttagtac gataggttca tttatttttt ggggatatct
      661 gggttctatc caaaaaacta tatctaaaga agaaggagta actaattgaa actattttta
      721 ggtatatgaa agtagaaaag aaaactagtt atcccaactt caacaaattt tatagttttt
      781 acaatataaa aatggtaaac taatattatt tgatacgaaa atgtgcaatt ggagtgaatt
      841 tgatggacac tatattgatt actggcggag ctggttttat cggttcaacg ctagcgaatt
      901 atttgggaaa agaaaacaaa atcgtagtag ttgacgattt atcaatggga aaaaaagaaa
      961 acttggatat ggataaacaa gtgactttca ttgaggggga tgtatcagat tctcaattga
     1021 tggaaagaat catgaaagaa tatcagtttg cctatatttt tcatctagct gctgtagcta
     1081 gtgtggcaga ttctgtagaa cggcctgtag aaacacatcg tgtaaatttt gacagtgcgc
     1141 ttttattgct tgagttagca cggaaatatc aatctaattt aaaacgatta gtatttagtt
     1201 catcagctgc agtttatggt gatgaaccaa cactgccaaa aaaagaagaa tcagtcatcc
     1261 gtccattaac accatatgct atcgataaat ttgcagcaga acaatatgtt ttggattact
     1321 gtcatttata tgatgttccg actagtgctg tccgattttt taacgtttat ggtccaaatc
     1381 aaaatccaaa ctctccatat agcggagtca tttcgattct agtggatcgt tacaaaaaac
     1441 aattggcagg agaaaaagcg gaatttactt tatttggcga tgggagtcag tctagagact
     1501 ttgtttatat agaagatgtt atacaagcac ttttacttgt tgccaaggaa gaaaaagctt
     1561 taggacagca attcaatgtc ggtacaggaa aaagcactac gctacttgaa ttgatccatt
     1621 caattgatca aatcttagga acagagctag cgcttaaata tgaagcggag cgttcagggg
     1681 atattcgtga ttccttagcg gatatttcaa aaattcgttc tttaggctat cagcctaaat
     1741 ttgatatatt gagcggaatg gaacggtatt taaagacaga aatttgatga aaaaatgatt
     1801 gcatctttgg ataaataatt ctaaaaag
//
LOCUS       DAKBVG010000289         1797 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.296, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000289 DAKBVG010000000
VERSION     DAKBVG010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.296"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            <1..487
                     /locus_tag="OUD09_002628"
     CDS             <1..487
                     /locus_tag="OUD09_002628"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333370.1"
                     /note="Catalyzes the rate-limiting step in dNTP synthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     alpha"
                     /protein_id="HCT9693849.1"
                     /translation="KFIEVNAEIVSEYLEKNPEKTADNLPPFFVTAMELSPKDHVSVQ
                     CTIQRWVDSSISKTVNAPKGYQVKQVAAIYEDLYQGGAKGGTVYVDGSRDSQVLTLEA
                     DGNVFEEPVVGVADNQITLDSLESSEAEEICPICHEGRIEEIGGCSTCTNCKVQLKCG
                     L"
     gene            501..1064
                     /locus_tag="OUD09_002629"
     CDS             501..1064
                     /locus_tag="OUD09_002629"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HCT9693850.1"
                     /translation="MKIYTKSGDKGNTSIIGGERIKKSDARVCAYGSVDEVNSWVGKI
                     ISELDDKKFELLKKELVVLQILLFDIGTDLATPIGIKEMIVGKEEVTKIETLIDFYQA
                     KVPVIEKFILPGGHPVACDMQITRTVIRRAEREITRLIVSDEPINLHAYKIMNRLSDL
                     FFVLARYVNHVYATKEPFYERAGKVFH"
     gene            1080..1484
                     /locus_tag="OUD09_002630"
     CDS             1080..1484
                     /locus_tag="OUD09_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HCT9693851.1"
                     /translation="MKKLATLLTVFIGLFIMTGCQSNQNDQASSKASTQATSEVAKSS
                     ALIKLTDSDKEITSKTVTYKSGESLLVILKANFEVKERDGFITSIDGHAQDESQNKYW
                     TFTINGKMGEKGAGETTLDNDDQVVFNLGVFK"
     gene            1686..1757
                     /locus_tag="OUD09_002631"
                     /pseudo
     CDS             1686..1757
                     /locus_tag="OUD09_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314380.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
BASE COUNT          645 a          256 c          369 g          527 t
ORIGIN      
        1 aaaattcatt gaagttaacg ctgaaatagt cagtgaatat ttagaaaaaa atccagagaa
       61 aactgcggat aatctaccac cattctttgt cactgccatg gagctttcac caaaagatca
      121 cgtgagtgtt caatgtacga tccaacgttg ggtagatagc tctatctcaa aaacagtcaa
      181 tgcacctaag ggttatcaag tgaaacaagt agctgcgatc tatgaagatc tgtatcaagg
      241 cggtgctaaa ggtggaactg tctatgtgga tggtagcagg gattcgcaag tgttgacact
      301 tgaagcagat ggcaatgtat ttgaagaacc tgtagtaggg gtggcagata atcaaattac
      361 tcttgactca ttagaatcat cagaagcaga agaaatatgt ccgatttgcc atgagggtag
      421 aattgaagaa attggtggtt gcagtacctg cactaactgt aaagtacaac taaaatgtgg
      481 cttgtaaaag gagctattcg atgaaaattt atactaaaag cggcgataaa ggaaatacta
      541 gtattattgg tggagaaaga ataaaaaaat ctgacgctcg cgtttgtgct tacggtagcg
      601 ttgatgaagt aaattcttgg gtcggtaaaa ttatttcaga gttggatgat aaaaagtttg
      661 agctgttgaa aaaagaacta gttgtattac aaattttact ttttgatatt ggaacagact
      721 tggctacacc catcgggatt aaagaaatga ttgtagggaa agaagaagta actaagattg
      781 aaacgctgat tgatttttat caagcaaaag ttccagtcat tgaaaaattt atcttacctg
      841 gaggacatcc ggtggcatgc gatatgcaaa ttacacgaac agttattcgt cgggcagaac
      901 gtgaaatcac acgattgatt gtttcagatg aacctatcaa tttacatgca tacaaaatta
      961 tgaatcgctt atcagattta ttttttgtac ttgcacgtta tgtaaatcat gtttatgcta
     1021 ccaaagagcc attttatgaa cgagcaggga aggtattcca ttaagagagg gttttaaact
     1081 tgaaaaaatt agcaacatta ttaaccgttt ttatcggact attcattatg acagggtgcc
     1141 aatcaaatca aaatgatcaa gcctcttcaa aagcatccac tcaagcaaca agcgaggttg
     1201 ctaaatcaag cgcgctgata aaattgactg atagtgataa agagatcact tcaaaaacag
     1261 tcacttacaa atcaggagaa tcattattgg taattttaaa ggctaacttt gaagtaaaag
     1321 aaagggatgg ctttattact tcaattgatg gtcatgcgca agatgagagt caaaataaat
     1381 actggacatt cactatcaat ggaaaaatgg gtgaaaaagg cgcaggtgaa actacattag
     1441 ataatgatga tcaggtagtt ttcaatttgg gtgtatttaa ataaaaatta tcgttcttta
     1501 cagcaattga gcgataatta agtaagagaa aattattagt ttttaaagga atatgttaat
     1561 tttaaaacac gttcaaacca atcatttcga aggggttgaa cgtgtttttt ggttctgttg
     1621 caaagtttta tctaaatacc tatttagtgt aaaatagctg aaactaaaac ataaggagaa
     1681 ttaccatgtc caattttaaa ggtaaacaat ttgaaaagga tattatcatg gtagccgtat
     1741 gctactattt aagatatagg ttctgttgca aagttttaaa taaagaataa aatccct
//
LOCUS       DAKBVG010000290         1763 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.297, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000290 DAKBVG010000000
VERSION     DAKBVG010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.297"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            <1..1332
                     /locus_tag="OUD09_002632"
     CDS             <1..1332
                     /locus_tag="OUD09_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HCT9693852.1"
                     /translation="FSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPL
                     TTVIYFAVDLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYAS
                     FAFVSDMSTGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELS
                     NDTNNDNYIAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISS
                     PVTEHDRKIYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDS
                     VSDFAGWLGDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTL
                     AKRNSDSTALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILG
                     VGIISFFIDKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            1412..>1763
                     /locus_tag="OUD09_002633"
     CDS             1412..>1763
                     /locus_tag="OUD09_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693853.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRF"
BASE COUNT          613 a          216 c          342 g          592 t
ORIGIN      
        1 ttttctaaaa atttatctgt gtttcctatc tatcaagatg gaggtgcagc attagcttat
       61 tttacttatg atcgtggctt atcagatggt aagaaggcta tagatgcagc aaaaaatcta
      121 aatataccat taacaacagt tatttatttt gctgttgatc tagacatgtt aggggaagaa
      181 attcttgcat atgctggaga atattttaag ggggtatctg ctgtaatgtc atatagcggg
      241 tatcaaacgg gtgtttacgg aacaagaaat gtttgttctc aaataatcaa taacgggtat
      301 gcttcgtttg catttgtttc agatatgtcg actggttata gtgggaattt aggttttcct
      361 atgcctagac aatggtcttt tgatcaattt gttgaattta ccataggaag tggcaacgga
      421 gctgttggta tcgatttaat cgctacatcg ggtagagata gcggatttaa tgagttatca
      481 aatgatacta ataatgataa ttacattgcc aaatacaatc agaaggttat aaatcagatg
      541 gtacgtgcgt atcaatatct atcacaagct ggtcttgata atccttggaa ccctcatttg
      601 acattttata gatatgtaaa ttattctggt ttgtcatggg acatcatttc ttctcctgta
      661 acagagcatg atagaaaaat ttatgatgaa tataggaata aattaactaa tagcgagggc
      721 ctgtataatt attttataga tcctaatact ggttcaataa taggtttgcc tcacttaatt
      781 gtgacgttac aatcacagat gtttctagcc gacacaatca atgatagtgt ttctgacttt
      841 gcaggttggt taggagattt aatgacttgc tggggtgaag ttaagaaatt aggaatacaa
      901 atggagcaag gtgtatttgc attagtaggt acggctgcag gtggaacatt tagtctagaa
      961 gatttatacc aagatgttga cgcggtaaat gtttatacct tagcaaaaag aaattctgat
     1021 tcaacagctt tatcggcttt aaaaacgtac tattgtgata agggtgctat tgatagattt
     1081 aatcaatttg ttaagaacag atttggttct tcagataaaa tcgcttcagt aacaaatagt
     1141 ttattagaaa atgacgaaac agttgatatc ttaggtgtag ggataatttc atttttcatt
     1201 gataaaatag ttaataattc acttgggaca gattttactt atcttgattt tattggagat
     1261 gaggggaaag ttgcaaatgg attttctgaa aaaattattc aatttcaaga aaatagaagt
     1321 aaacttaatt aagaatcgtt tactatttaa acagtgtcct tttccttttt aaatatatac
     1381 gataaaataa gtatatgaat tgtaaaagga catgatgatt atggaagaaa tagaatggaa
     1441 taatctgact caaaataata aaaataattc tggatgtgga attatcttta tgtttgttat
     1501 agttttggga atagtagtac ttttaataac tgtcaagata gtagattact ttgcgaatac
     1561 tcctgaagaa caaatacaga gaaaacttcc tgagtttgaa agagtaccac cttatgatcc
     1621 aagagataat tcatttcaag taaaaaaatt aaaagagtct ccaaatgaaa tgtggtttga
     1681 agttacagtt gaatcaagcg acttcaaaga aaaaaagaaa gaatacaatt atattgtaaa
     1741 ggcaaaagta tcaaaaagat tta
//
LOCUS       DAKBVG010000291         1753 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.298, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000291 DAKBVG010000000
VERSION     DAKBVG010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.298"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(135..208)
                     /locus_tag="OUD09_002634"
     tRNA            complement(135..208)
                     /locus_tag="OUD09_002634"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(172..174),aa:Ile,seq:cat)
     gene            complement(397..1416)
                     /locus_tag="OUD09_002635"
     CDS             complement(397..1416)
                     /locus_tag="OUD09_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HCT9693854.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1427..1633)
                     /locus_tag="OUD09_002636"
     CDS             complement(1427..1633)
                     /locus_tag="OUD09_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HCT9693855.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
BASE COUNT          506 a          365 c          302 g          580 t
ORIGIN      
        1 aaacatcctt tcattaatcc ataaaaggat acataaaaaa gccactcatt tgagtggcaa
       61 tgaagaaaag ctttagcttg tataatactc ttcaaaaaaa ttctaacaca gaacgattca
      121 aatggctacg ttaatgtacc ctgtaggact cgaacctacg accggacggt tatgagccgt
      181 ctgctctgac caactgagct aagggtactg gttgttgcca cataaagcca taaacaatca
      241 accagtagaa tgtgtggcaa caaacctgtt atcgcatagc ttggagtgtg actattatgg
      301 gtgatagtga agatatgcga taacatcact attttatcga atgattttta tagttgtcaa
      361 tatagttatg taatgctcct caacgaggag ctatttttat cgtttaggaa tatttaaata
      421 ccaacgtttg tcatggaaat cttgcgcacc gcctttagtg tttccctctg gatcattcgt
      481 tgcccgcatc atgacataga ctttcttatt agggaagtta cgcatgttaa aagatacatg
      541 atagccaaca tttccagaag tattataagc ttgatttaca tctgatctat aaattccatc
      601 agctcttact cgagctaatt ctttcccagt attgtaatcc ataatgaaga tatactcgta
      661 tttatagtta gcaatgtgcc atccagctac atgcaaattt gcattttcga tttctccaaa
      721 ctgatcaatg tgggcgtaat tcgttccatc tgtcagtgta ggattagctg caccagctcg
      781 tgttggatca atgacaggct tgttttcaga agttgttgga ttttcatcgg taaatccatg
      841 agctaaatca tatgctaatt tttctttact tacgcccatt tcagaaagat aaccgtaagg
      901 atctgtatga tcgccccaga tgttttgtgt tacccataaa tgcgatttga ttcccggttg
      961 gttataagga gtgtccaacg ttaatggaat accatatttc attgctgaat ctctagccaa
     1021 ttcaacgtat gccttgtagt ttttctcaaa cgttgcttta tcatgtgtgt gttgtaactc
     1081 aatctgcaca ggactgttgg cattagcata cgaaccagca ccgtactgta cataaccagg
     1141 ttgaccgact tgataaacaa ttccgccgtc tcccacaata taagcagtat aagcgctagt
     1201 ccatgaacgt tgcatatact gcgcttcatt gcgtcctgtt gctgtttcat tagccgtttc
     1261 atgcagtaaa atatactgat tatttgctac ttgtgagcta ccttcatttg cgcccaaatt
     1321 aaattcattg ttgatagtat aggcaaatcc gttaattggc aataaaaaaa gagccgttaa
     1381 caggctcatc gcagtaatag taattttctt tttcatatct atcctcctat ttatctgatg
     1441 aaattttctt atacgttcga tttgatacac ctaaaatact ccctagaaat gctccaaaac
     1501 ctgtaataat catcaccgtt atatctgtgt accgccagtt aacacctttt ccgacaagac
     1561 cgacaaaagt cgctaaagcg gggataacta ctatagcgat ccatttcaaa atatcaaagg
     1621 ttctattgtt catactatcc acctcccttc attggttggg ctactttctt tttcaatcgc
     1681 ttgttttctt ctatcaatat ttctctttct gaaagcaagg catcaatttg cccgtccagt
     1741 tgttcaattt tat
//
LOCUS       DAKBVG010000292         1753 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.299, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000292 DAKBVG010000000
VERSION     DAKBVG010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.299"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(415..>1753)
                     /gene="brnQ"
                     /locus_tag="OUD09_002637"
     CDS             complement(415..>1753)
                     /gene="brnQ"
                     /locus_tag="OUD09_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HCT9693856.1"
                     /translation="SFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWANLGFL
                     VTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPRLATTS
                     FEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLVLLGAL
                     LLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTIQNMGV
                     TKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIADHYLG
                     TYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLAANVGL
                     TNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDGLNACP
                     ETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD"
BASE COUNT          619 a          347 c          306 g          481 t
ORIGIN      
        1 tttgcgaaaa ctatttttat atcatatata tgtctaaaat ctatattata aaagaaatgt
       61 tgtttgatca gcttctcttc ggtattagtt caaaacaaaa cggcaaagta tatggagcta
      121 ctttgccgtt ttgtttctct aatactcagg tcagtttata gacgctcaat aaacggtgtt
      181 caaaagccag tttctcggta ttaagtcaaa tttatgcaaa aatgtgagaa gccattttca
      241 caaaatattt cttaatactt gaaactaagc ggctttttca cgacctctca ataaacggtg
      301 ttcaaaagtc agtttctcgg tattaagtca aatttatgca aaaatgtgag aagccatttt
      361 tcaaaattac tttttattgc tcaaggctga acgtctcttt cacaacctct tttatcaatc
      421 tgctacttct tctttcttag cgaaactcca aatcagtccg ataacaaaac cgataactgc
      481 tggtacgatc caccccatcc ccaatttgaa aaacggcaag tagctttccg caacatataa
      541 aagattttga acaataggtg tttgtttgat agtttctggg caagcgttca acccatcaaa
      601 aatcgaagca atcaatgtaa aataagtagt cattcgataa acagccgtac gctgcttaaa
      661 caaaggacca actaacacta gtaaaatcag tgtcattgct aatggataga tgaacatcaa
      721 aacaggcaac gaaaattgaa tgatattcgt caagcctacg tttgccgcta aacaaggaag
      781 gatactagca agtgcaacaa agaatgcgta atttctttgc ggaaacaaat ctgtgaatgt
      841 ttctgcaaaa gctgtaatca aaccaatacc ggtcttcaag caagctaata taacgataaa
      901 tgctaataag atactgccat aagtacctaa ataatgatca gcaatctgag ccagcgtgat
      961 cccgccattt tcacttagag caaagctacc taagctcata gcacctaaat aagcgagcaa
     1021 agtataaatg attcccatca atataatgct gatcgcacca gatttgatcg tgtctttagc
     1081 gatttcagca ggttttgtta cacccatgtt ttggattgtt gtgacaatga tgatcccaaa
     1141 tgctaaagca gcaagcgcat ctaatgtgtt atatccttga gtaaaccctg tgaaaaaagc
     1201 atgctcttga taatttggct gaataggcgc ttgatttaca gaacctagtg gattcaaaaa
     1261 agcaaggaga agcaaagcac ctaataaaac caaaaatgca ggattcagga actttccaac
     1321 ataatctaat atctttgtcg gttttcttga aaaccaccaa gccagcacaa aaaatacaat
     1381 actgaaaatt gctagatata gcgtctgtct gctaggatcg ataaatggtg ctaaaccaat
     1441 ctcgaaagat gtggttgcaa ggcgaggtaa agcaaaaaac ggtccgataa ctaagtaaag
     1501 caagatggta aaaaccatcg catatgtttg atttatccgt tttgctaatt catatacacc
     1561 tgagcttttc gatacaccaa tggcaatcac ccctaagaag ggaagaccga taccggtaac
     1621 caagaaacct agattcgccc aaaaggtagc agcgcctgct tcttgaccca aatgaacggg
     1681 aaagataaga tttccagcac caaaaaatag accgaaaagc atggagccta taaaaagata
     1741 gtctttaaat gat
//
LOCUS       DAKBVG010000293         1749 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.300, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000293 DAKBVG010000000
VERSION     DAKBVG010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.300"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(68..1600)
                     /locus_tag="OUD09_002638"
     CDS             complement(68..1600)
                     /locus_tag="OUD09_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCT9693857.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
BASE COUNT          459 a          332 c          336 g          622 t
ORIGIN      
        1 ttcagaagaa ggagttgtga caaaactttt gtcacagctc ctttttttca actattcaat
       61 caaacgttta ttttggttca atctcaatga ctcgttgcac aagatcttga taaaattctt
      121 gttcatgaga gacgatgatt actgttcctg gaaatttctg tatcccgtct tgtaagcttt
      181 ctttcgtttc ttgatcgata tggttcgtcg gttcatcgag aatcagtaaa ttaccttggt
      241 tcattgtcaa ctgtgcaagt ttcaccttag tttgttcccc accacttaat gtagtgagag
      301 gttcacttgc taattgatta actaaccctg ctttagctag ttggcgacgt aattctttga
      361 tagtcgcttt tggaaattga ctgcttaaat attgtagtgg tgtttctaat ggattttccc
      421 agtacagatc ttgtgaaaag taattgattt ttgtgttatc tgggaagtgg aaatcgccac
      481 ttattttttc aatctcaccg atcagtgttt ttaataaagt agattttcca atcccgttga
      541 agcctcgaat ggcaatattt tcaccaaagc ggatggtcaa attgatgggt gcaagtagcg
      601 gtcgatcata tccgatgact aaatcagttg tttccactgc taatgtcgtg acaataggcg
      661 agaatggaaa atcaaagaca gctttacttg ctgaagctgg aggcgtcagc cgttcgattt
      721 tatctaattg tttttgtcgg ctttttgcca tcgttgatcg ggaaccggct ttgtattttc
      781 gaatataagc ttcggtcttt tcgatttgtt tttgttgagc atgatattgg cgcaaatagc
      841 tttcgcgatt ttgttccttt tgtttcaatg cttgttttag atggccagta tacttagtaa
      901 tctttccaaa ctcgatgtct gcgatatggg tggtgatctg atcaagaaat tcttggtcat
      961 gggaaatcac gatatacgca ccgtcaaaat cctgaagaaa ctgcgtcagc cattggatat
     1021 gctgatcatc taagtgattg gttggttcat ctaagatcag aacatctggg ttttctaaaa
     1081 gtagttttgc taaaatcacc tttgaacgtt gtccaccact tagatttttc aacagtgaat
     1141 ctaaacctaa gacatcgatc cccaacccgc tgctcatctc agcaactaag gtgtcgattt
     1201 gataaaacac tccttggtct aattccgttt ggagtttccc agctttttcc agcaaggaat
     1261 catcgaaggt ctcgctatat gtttggtaaa gaaaagcaat tttttcttcc ttttcgtatt
     1321 cttttgcgta cgcagttttt agaaattctt cgattgttaa tgtttcatcg acttccacat
     1381 attgatcgag gtaacctttg tgaatatttt tttgccaatg gatctgtccg tcatctggta
     1441 agatctcacc agtgatgatt ttaatcaatg tacttttacc ggctccgttt tgtccaatca
     1501 gcccaagatg ttcgccagca tttaattgca gatcgacacc ttcatagagg attttgtcgc
     1561 caaattgctg ggtaatgttg tttaatgtca gtatactcat aataactcct ttttcataaa
     1621 aaaactgcag acataaagcg tccacagtga atcacttgct tggttttcat gcaacatgga
     1681 ttgtttgatt gaaccatctt atgaaaccta acacggattc ctatttattg tcaagaaaat
     1741 gaaaaaaat
//
LOCUS       DAKBVG010000294         1731 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.301, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000294 DAKBVG010000000
VERSION     DAKBVG010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.301"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            27..539
                     /locus_tag="OUD09_002639"
     CDS             27..539
                     /locus_tag="OUD09_002639"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCT9693858.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLY
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            536..1573
                     /locus_tag="OUD09_002640"
     CDS             536..1573
                     /locus_tag="OUD09_002640"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCT9693859.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT          618 a          333 c          369 g          411 t
ORIGIN      
        1 taaagactat acggaggatg attaccatga caaataaaaa caatgaacgt tactctaccg
       61 aactgaaagc gacagccatc aagaaaatga tgccacccga aaataggggg attcgccaat
      121 taagtcaaac tttgggcatt ccagaagcga ctctttattc ttggcgtaaa aaagctagaa
      181 ttcaagggaa tactacaccc ggaacgaaaa aaaatgtcga aaaatggtca agtgaagata
      241 aatttttaat tgtgatggaa tcattctcaa tgaaccaagt agaacttgcg gaatattgcc
      301 gaaagaaggg cttatttaaa gaacaaattg aggcttggaa gaaaacgtgc ttatctgcga
      361 atgaacagga agaaaatcga acgagagaac ttgcaactga actaaaagaa gagaaaaaac
      421 aagcgcgtca attagagaaa gaattacgtc ataaggaaaa agcattagcg gaagccgctg
      481 ctctcttatt actaagaaaa aaagcacacg cgatttgggg ggaccaagag gaagaatgat
      541 ttcatcttca gatcgcaaac aagctgttga acttattcaa gaagccaatc gcaatggggc
      601 ccgtctcacc tatgcctgta atgagctgaa tattagcgtt cgaacctacg aacgatggac
      661 gcgtgaaggt acgatagcgc atgatcaacg ccctttggcg aaacgtcctg tacccaaaaa
      721 taaactgacg gatcaagaac gcgaaaaaat aatagaaacc gtttcaaaaa aagaatttat
      781 gaatttgcca cctagccaaa ttgtaccaaa acttgcggat tgctcgattt atattgcatc
      841 agagtctagc ttctatcgtg tattgcgaga aaaaaatatg caacatcacc gtggccgaag
      901 tcaagtttca caaaaacgca taccgccaag tcaccttgcg acgaagccaa atgaagtttg
      961 gacttgggat attacttggc taaaaggtcc aataaaagga ctcttttacc ggttatatct
     1021 catcattgat ttatttagcc gtaaaatggt aggctgggag atttgggaat cagaagaagc
     1081 tcgtcatgcc gaaaaactta ttaaacagac ggtgcttaag gaacgtatcc aaggacagcc
     1141 gttggtttta cactccgata atggcagccc tatgaaagcc gcgacttttc aagtcttact
     1201 cgaaaaatta ggaattcaaa gttcttattc aagaccacga gtgagtaatg acaatcctta
     1261 ttcggaagcg gctttccgaa cgttaaaata tcgaccagaa tatcccaata acggctttaa
     1321 aacaatagat gatgctcgaa aatggacgaa agagtttgtc gattggtata acgattctca
     1381 tttgcatagt gggattcaat ttgtgtctcc tgcagcgtgt catgaaggac gacatctcgc
     1441 tattttagag aaacgaaagc aggtttacga ggacgctaaa aagcgccatc ctgaacgctg
     1501 gtcaaaaggt gttcgaaatt ggactccaca tcaatcggtg gcgttgaatc ctttaaaaga
     1561 aacagaaagt taacaggagc ataatctttc aagattatca aataaccctc gtcacccaat
     1621 aggggaaaaa gttattcttc ccctattgga taacgaggcc aaccgaccat agggaggttc
     1681 tgctttttga taggtggaaa ataaaaatac gacaactata ttgacaaaca c
//
LOCUS       DAKBVG010000295         1694 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.302, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000295 DAKBVG010000000
VERSION     DAKBVG010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.302"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            276..887
                     /locus_tag="OUD09_002641"
     CDS             276..887
                     /locus_tag="OUD09_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HCT9693860.1"
                     /translation="MIDIISAITVNVLTALYQPFLFAVLTAYLFMYWYLFIVDNSRDP
                     KNLKDSVLIWLRYFKASSKFRRLFFLAFYFTLILFKTLLNRNMWMNPLSNVIGQWSLY
                     IIDNATGESRLTTECVENTMLFIPFCMLLLWWRDIKCDIIRTIYVGIKYVFLFSLSIE
                     FTQLFFRLGTFQLSDLFYNTLGGLIGALLYWLFYRLNKYIDLK"
     gene            1069..1584
                     /locus_tag="OUD09_002642"
     CDS             1069..1584
                     /locus_tag="OUD09_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HCT9693861.1"
                     /translation="MVVVMVKVFDIIKFSDFRGYTAVALDNETLFSIGIEFHIKQINQ
                     GFSHNKYTMRGLHYQSGEHAQGKIVMCLHGEIMNIALDIRQGSSTYGCVYVNTLSESN
                     GKCAYIPRGYAHGYLTMEDNTLVQWCVDNDFNSNAAKCISYESVDIEWPVSRQCFVIS
                     EKDKNVERLSF"
BASE COUNT          551 a          218 c          331 g          594 t
ORIGIN      
        1 tatgtttttc tgcgtcatcc attatatttt ttatagaaaa gtttgcaaag aactattgga
       61 tggaaaaaac atatatgatg tcagaggaat acttttgatt tctgcaatca cgatagctgg
      121 tggcatccta atttcaataa ttaataaatt tgatttcata aaatatttaa ttatagcaat
      181 tgcttgtgtc gctgcaatta tatttagaaa acgattgcta cttttagtaa ggagaattat
      241 gagcaaataa acatttgctc atagagagat agagaatgat agatattatt tctgctataa
      301 cggtaaatgt attaacagca ttatatcagc cgttcttatt tgccgtatta acagcgtatc
      361 tgtttatgta ctggtatcta tttatagttg ataactctcg agatcctaaa aacttaaaag
      421 attctgtatt aatctggttg agatatttta aagcatctag taaatttcgc agactctttt
      481 ttcttgcgtt ttattttact ttgatattat ttaaaacctt gcttaatcga aatatgtgga
      541 tgaacccgct ttcaaacgtt attggacagt ggtcattata tattatagat aatgctacag
      601 gagagagcag attgacaaca gaatgcgtgg agaacacgat gctgtttatc cctttttgca
      661 tgttattgtt atggtggcgt gacattaaat gtgatattat acgcaccatc tatgttggga
      721 ttaagtatgt gtttttattc tcattaagta tagaatttac acaactgttt tttaggcttg
      781 gtacatttca actatcggat ttgttttata acaccctagg gggcttaatt ggtgctttgc
      841 tatattggct attctaccga ttaaataagt atatagactt aaaatagaac tgctttgaat
      901 tttaatatgg ttaatatttt tctgcagaca ggattgtgta atctcatcaa gaatacttta
      961 catgccatct atttttgcta aaacattgtt gttatatctt caagaggtag ggaattttat
     1021 tgctttaaat gaggtcgagc gaattaaaaa attggtaaaa tatataacat ggtggtagtt
     1081 atggtaaaag tgtttgatat aattaagttt tcggatttca gaggatatac tgctgttgca
     1141 ttggataatg agactctttt ctcgattggt attgagtttc acattaagca aataaatcaa
     1201 ggatttagtc ataataaata tactatgcga gggctacatt atcagagtgg agagcatgct
     1261 caaggaaaga tagtgatgtg cctacatggt gaaattatga atatcgcact tgatattcga
     1321 caaggatctt ccacttatgg atgtgtgtat gtcaacacat tgagtgaaag caatggtaaa
     1381 tgtgcgtata tcccacgtgg atatgctcat ggctatctta ctatggagga caatacgcta
     1441 gttcagtggt gcgtagataa tgatttcaat tctaatgctg ccaaatgtat aagttatgaa
     1501 agcgtagata ttgaatggcc ggtcagtcgg cagtgtttcg ttataagtga gaaagacaaa
     1561 aatgtggaaa gattaagctt ttgattaaaa tgagctgtgg agaaaagaag gatactggaa
     1621 aataagaaga acaataaccg agcaaatgct cggttatttc tatgaggaat acataaaact
     1681 aaatttagat atag
//
LOCUS       DAKBVG010000296         1688 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.303, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000296 DAKBVG010000000
VERSION     DAKBVG010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.303"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(896..1414)
                     /locus_tag="OUD09_002643"
     CDS             complement(896..1414)
                     /locus_tag="OUD09_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HCT9693862.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT          531 a          321 c          232 g          604 t
ORIGIN      
        1 aaaatactaa agttatttgt tcttttatta aatacaccta caacttataa attgatggta
       61 tttagataac acgaaagaat aaaaataaca gctgatagta aagtttgttg acgattgcta
      121 ctcaatcgaa agtgagttag tgcgtatgaa cttgcttctg aaaacttatg aaatgaaaga
      181 ccttttacat cttgcagaaa tcggtactgg gattaaacta agcaataaaa actaatgttc
      241 ccctcattct ttgaacttct actatttgat ttatttattc tgataaatga cgatttcacc
      301 ttcaggatct tcaaatcccg tatagaattt atttaagatc tcttcagtat cagtaatagg
      361 ataggcgata ggccacatac cctttgatgg ttagaataaa ctgctcaaaa tctgtatcgt
      421 atgttggctt tagcatatta acaaaggcta tctccaaatt ataaaccata agcgttgaat
      481 cgttcttaac aatttcaatt cttcctaacc caaagtcaga agatataatt accggacttg
      541 ccttaccctt catgtgggtt gttgagattc catatttaaa gggaattgtc ggtactatcg
      601 gcggtctgaa aaacatccca aaaataaaaa aatctttccc ctgctagtct cctaagtccc
      661 tttgtagcgg atatggcacc ataatctcat tttatcgaag tgagtcatgg tttatctgtt
      721 ggccaaccaa aattgataca atctaacgat gcaccctcta cggcattcta ggggagtgca
      781 cttgcattca cccaataaaa accggcagtt agcaaagaac ttatgtagtc gtatgttctt
      841 actgttaaca tacaagaggt ctcctttcca caattgctta gcagtaaacg ggaatttaca
      901 aagctaatct cttccatttt cttatctttg ttctaaaagt cccaaatgga gcaactgtat
      961 ttacatgaat aaatttatat acttcccata cagccgtttt ggttgcctcg tctgcccatt
     1021 ttcttttgtg tgcagtgaat agatcttcat cactcattga atcaatcatc atgttaatat
     1081 catcaatatt acgattgaga atttcctcta attctttcaa tggtagatgt gcataggtat
     1141 ttgtaaacca cttatataac tctcctaact gattccattt aaattgatca gaaggcgttt
     1201 taacagataa tccatttctt tcatcatttt cccattttag aattaaattc gtccaaccaa
     1261 cttgatatgc taaattttca gcaggtgttc tatccacttc ctcaactctt ttatccttca
     1321 attcttcagg aatagtttca aattctaaaa tatatttagt ataagtccta tttatttctt
     1381 tttttaattc ttctttattt ttatatgttc tcattatatt acctccataa attcctaatt
     1441 gtctccttta attcatctat aatttcatcc acgatatttt aatccgttct acaatgcata
     1501 tctgatatgc attgactgtc taactcatat gtgaatgatt tataaaatat cccaacgtct
     1561 catctaaaga aatgttgatc gtcagcaagg agctaaaaaa tacgaatata tttcttttgt
     1621 agtaaaatcg gactggtttg catatcattt tcttcgtaaa tatcaaaatc atgttaacta
     1681 ctatcaat
//
LOCUS       DAKBVG010000297         1659 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.304, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000297 DAKBVG010000000
VERSION     DAKBVG010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.304"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(<1..635)
                     /locus_tag="OUD09_002644"
     CDS             complement(<1..635)
                     /locus_tag="OUD09_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCT9693863.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQ"
     gene            complement(646..>1659)
                     /locus_tag="OUD09_002645"
     CDS             complement(646..>1659)
                     /locus_tag="OUD09_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCT9693864.1"
                     /translation="ELKPKHERQNFLVRDDRLDHAVAFLWKDPQTNETVGASYQGTFI
                     DYERFGERGTYKHIDKNSTANHGFNLKIGDPKQLKFFESSIDLLSYAALNRDQLNDTW
                     LVSMEGLKHHVISHYFGEAVSELRKKQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDP
                     FTNQKVRCERGIANDWQVPKEYKVIYEEVAKEEKITPEAIMAIHKTENNLQLTNQLVS
                     AHKVNASFGQQLSVNDSIEAINLKDICREVAKELKACERVDGTYDFDRFYQEKGDINA
                     QILFSYKAEQYYKGYKNHEHEFVPEVKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQ
                     ERE"
BASE COUNT          434 a          356 c          287 g          582 t
ORIGIN      
        1 aattgataaa tcatgtaaag cgttggggtt tggactctgc ctaaggaaaa acttcctggt
       61 atgccttttt gctgcaagag taaactatac agcgggctac cgttcatgcc gatcaaccaa
      121 tcggcgatct ggcgtgcttg tgcttcttca aattttgggt aggtctcttc tgcatctcgt
      181 aactgctgga atccctgata aatggcttct ttttctaagg agttgatcca aagtctttta
      241 aatatctttc catgatcggc gcctgcttgt tgaatgatcg tccaagctat cagttcccct
      301 tcacgatcgc tatctgtcgc aataataatg gtgtcggccg attgaagttg ctgtttcacc
      361 accttgaatt gctttttctt atctttagga acatgatagt gataatgtgt agggaaaata
      421 ggcaaatgct ctaatgacca ttgtttccaa ttttcatcgt acatatcggg cgaatctaac
      481 tcgactaaat gaccaaaacc atacgtgatc acagtttctc ctgaaaacaa tcgatcttgg
      541 atctcaaaat acccatcttt tcgggtagct ttagaaaagc tatcggcata agccttcgct
      601 tgtgacggtt tttctgctaa aataacggtt ttcatcatga cgatttcact ccctttcttg
      661 ctgtctacct tgttgaaaca acatggcacg tttttgcttt ctgatctctt gttggtagat
      721 ttcatgcttc aattgatcat tccaatcctt ttttacttca ggtacaaatt catgttcatg
      781 atttttatag cctttgtagt attgttctgc tttataagag aaaagaatct gagcattaat
      841 atcccctttt tcttgataaa aacgatcaaa atcgtacgta ccatccacgc gttcacaagc
      901 ttttaattct tttgcgactt ctctacaaat atctttcaaa ttaatagctt ctattgaatc
      961 gttaacagaa agctgctgac caaaagacgc atttactttg tgagcggaaa ccaattggtt
     1021 cgttaattgc agattgtttt ctgtcttgtg tatggccata atagcttctg gtgtgatctt
     1081 ttcttctttt gcgacttctt cataaatgac tttgtattct tttggcactt gccaatcatt
     1141 agcgattcct cgttcacaac gtactttctg attcgtaaat ggatcaactg cgcccattaa
     1201 ttgttctttt tcgtagaaaa tatgtcctgc acggtcatta tccacacaaa tctcgatgga
     1261 ttgaggaaag gcttgtttct ttctcaattc agatactgcc tcaccaaaat aatgactgat
     1321 cacatggtgt tttaaccctt ccatcgaaac taaccacgta tcgtttaact gatcacgatt
     1381 tagtgcagca tagctaagta aatcaatact actctcaaaa aattttaact gcttagggtc
     1441 accgatcttt aggttaaagc catgattcgc tgtcgaattt ttatctatat gcttatatgt
     1501 gcctctctca ccaaaacgtt cataatcaat gaacgttcct tgataactcg cccctactgt
     1561 ctcatttgtc tgtggatctt tccataaaaa ggcgaccgca tgatctaaac gatcgtctcg
     1621 aactaagaaa ttttgccgtt catgttttgg ctttagttc
//
LOCUS       DAKBVG010000298         1657 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.305, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000298 DAKBVG010000000
VERSION     DAKBVG010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.305"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            <1..338
                     /locus_tag="OUD09_002646"
     CDS             <1..338
                     /locus_tag="OUD09_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCT9693865.1"
                     /translation="NLDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGE
                     KQRIACARVLLKNSKIILADEPTGSLDKRNKEQVVEILSQQASQGTLVIVVTHDPYLT
                     KIGNKKIML"
     gene            715..1626
                     /locus_tag="OUD09_002647"
     CDS             715..1626
                     /locus_tag="OUD09_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCT9693866.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
BASE COUNT          660 a          252 c          324 g          421 t
ORIGIN      
        1 ttaatttaga tattggattg aagttttcga atttaaatgt ttcagataag cgaacagcaa
       61 agtatgaggc attgaaaaaa gtaaatataa acaagaagct aagtacgcct atctacacat
      121 tatctggtgg tgagaagcaa agaatcgcct gtgcgagagt cttattgaag aatagcaaaa
      181 tcattttagc cgatgaacca actggatcat tagataaacg aaataaggag caagtcgttg
      241 aaatcttatc gcaacaggct tcccaaggta cgttagttat cgtggtcacg catgatccat
      301 atctgaccaa aataggaaac aagaagatca tgctttagaa aacaaatgaa aaaacaaact
      361 ttttctaata aaaacaacaa aaaaatgtct ggaaaatcga acataaaagt gtctgatttg
      421 aatatcccaa cagcggaaga agaaaaacaa aatcgataac atttgttcta agaattagtt
      481 acgttaaata cggaacgcaa gatagacttg aaaaacggta acaaaagaag tagtatatta
      541 aggtgtgatg tgctacttga catttattct gctgagcgaa ctttctggat tgtgtagcct
      601 tatatctatt caggacgtat cgagcagatt agtaaaggtg ttcttgatga ttttcatctt
      661 gaacaccttt gtttttagca attcgcaaaa aacgaaaagt aggagaattg ataaatgaaa
      721 aaaagatttt tagcacttgc catcgtacta ggcactggct tattgtcagg ctgtacgaat
      781 gccggtgaaa aaacagccgt tagttataaa ggcggtacaa tcagcgaaca agaagtaatg
      841 gatagcttga aaaaaatgca aggagcagat tccgctgtgc aacagttgat cgtttatcaa
      901 gtatttgaag ataaatatgg agatgacgta tcaaccaaag aaatcgattc ccaatatgat
      961 caaacaaaga aacaattggg tgattcattt gattcacaat tgaaatctgc aggttacaca
     1021 gaacaaacat tcaaagacag tatcaaacaa agtctagcct ttcaagaagg tctaaagaaa
     1081 catatcaaat tgaccgatga agatttaaaa acggcttggg aatctttcca tccagaagtg
     1141 gaagctcaaa ttatccaagt agcaagtgaa gatgatgcaa aagatgtgaa gaaagctgct
     1201 gataagggag acgatttctc taaattagcc aaagacaagt caactgatac tacgacaaaa
     1261 gaagatggcg gaaaagtgaa atttgattcc actactacaa cggttccggc agaagtcaaa
     1321 gaagcagcct ttaagttgaa agatggtcaa gtttctgatg tgatcacttc taccaatgct
     1381 tcaacttata ctacggaata ttatgtggtt aaaatggtga aaaaccaaaa caaaggcaac
     1441 gacatggata aatacaaaaa agaattgaaa gaaattgcaa cagacacaaa attaagtgac
     1501 agtaccttcc agaataaagt aattggcgaa gtattgaaag acgctaatgt aaaaatcaaa
     1561 gacaaagact ttgaaaatgt tttgagtaca ttcacttcag attctagtac agcaagtagt
     1621 aaataagcgg tataaataaa aaatatgaac tatttaa
//
LOCUS       DAKBVG010000299         1654 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.306, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000299 DAKBVG010000000
VERSION     DAKBVG010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.306"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            132..506
                     /locus_tag="OUD09_002648"
     CDS             132..506
                     /locus_tag="OUD09_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HCT9693867.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(957..1238)
                     /locus_tag="OUD09_002649"
     CDS             complement(957..1238)
                     /locus_tag="OUD09_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HCT9693868.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLISRSTI"
     gene            complement(1372..1444)
                     /locus_tag="OUD09_002650"
     tRNA            complement(1372..1444)
                     /locus_tag="OUD09_002650"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1409..1411),aa:Thr,seq:ggt)
BASE COUNT          580 a          237 c          283 g          554 t
ORIGIN      
        1 tctacactct gagatgtaag aaatatcata agatatttct tacatctttt tgttttgagc
       61 aggactttag ataattttat taatatatgc tagcatgctt ttgtaagaaa aagaaagagg
      121 gcgtattagt tatgtatctt aaaattacgg aacaagcaca agaaagatta caaaaatata
      181 ttgatgaagg cgcaacagtt atattggatt tagatgatgg cgttggggaa tattctaaaa
      241 tgggtgtttg ctctttagat actagttttc gtttacttct tcttgataaa gaacagagaa
      301 aaaatgatta taaactccaa ttagatagtg atattgggga tgtttatata aaagattatt
      361 caaaaatgta catggatgaa catatgaccc tttctttaga tccacgtcta ggtgtattta
      421 ctcttgaaag cccaagtggt tctttagaca gtcatgttca attagtagat ctacgaccag
      481 aaaaattctc tggaataaaa caataacttt ctctctattg tccataaaac tagtctcaat
      541 caagctgtat tgaaaaaaca agacaacatt cttaccatgg taaataaaaa gacgacacat
      601 tttgtcgtct ttttttaaat taactatttt cgataaaaga tcgcatagct tgtgccatta
      661 tttctggctt ttctctattc aattcatgtt ttccgttttt aataaagagt agccggccat
      721 tcgttgaaag tttatataaa cgttttgctg ctggtttatt cgcatgatct ttggtaccgc
      781 ataaaatcaa tttaggaagt cgtgatgtac gaagctgttt tttgcagtct aatgtttcaa
      841 gtgaacgaag tagggtaagg gtttgttttt tagatagacc taatttttca aatatcttag
      901 aaggtacgca gtaaaaataa atattctgga aagtaagcaa taagcgatta ggagactcaa
      961 attgtggagc gactaattaa atatggagaa ggagttgcaa gatagtttaa agcaataaca
     1021 gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa ttgtacttcg
     1081 atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg attgaacaat
     1141 gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt ccatgattgt
     1201 tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt tttatagtta
     1261 tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg tcataaaata
     1321 aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg aagccacttg
     1381 ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc tgagctaaag
     1441 cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg tcaacagaat
     1501 tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa taaaaataac
     1561 aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa gtattgcgtt
     1621 atttagaaga aattgatatt atattcatgt actt
//
LOCUS       DAKBVG010000300         1644 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.307, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000300 DAKBVG010000000
VERSION     DAKBVG010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.307"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            <1..312
                     /locus_tag="OUD09_002651"
     CDS             <1..312
                     /locus_tag="OUD09_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HCT9693869.1"
                     /translation="QETVQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKES
                     SGSYTATNGRYIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGW
                     Y"
     gene            complement(636..719)
                     /locus_tag="OUD09_002652"
     tRNA            complement(636..719)
                     /locus_tag="OUD09_002652"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(683..685),aa:Leu,seq:cag)
     gene            1247..>1644
                     /locus_tag="OUD09_002653"
     CDS             1247..>1644
                     /locus_tag="OUD09_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCT9693870.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSV"
BASE COUNT          508 a          308 c          276 g          552 t
ORIGIN      
        1 caagaaacgg ttcaaacgga aacacaagca gcgcctgttg ctgaaacaca acctgctcct
       61 gctgtaacag aaactgctgc tactccagca agtacaagct ctgcaaaaga atggatcgca
      121 caaaaagaat caagcggttc ttacacagca acgaacggac gttacatcgg ccgttaccaa
      181 ttagattctt cttacttaaa tggtgactac tctgctgcaa accaagaaag agtagcagaa
      241 caatatgtta cttcacgtta tggttcttgg gaagcggcga aagcattctg ggaagcaaac
      301 ggttggtact aagattcatt atttgaacgt ttaaacacat tcaaaatata gtaagaggtc
      361 atgcgattta ttctttcgca tgacctcttt ttgagtttaa gtttagtctt tattccttaa
      421 ctatcgtttg ccagtatttc ttgtatcaga atctataaat ctactatttg aatcaatcta
      481 ttaggtttta aactgtccca ccttcacaca aatttttttt gatttatttg aatctggttc
      541 cgttgtttaa aatttttcct atattctttt agataatatg ttattttctt tctcatcgct
      601 ccaggtgtac cttctcttta taaaaaaaca aattatgcgg ctaagaagac tcgaacttcc
      661 acgaggtcac cctcactagc gcctgaagct agcgcgtctg ccaattccgc catagccgca
      721 tcacatcttt tagtttagga ttacatgtgt tttttgtcaa gaacatattt ttgttctttt
      781 tcatttctgt acgccctccc ttatgccatc tacacaaata attcatccga gcaactccat
      841 catctattga gggttggtga aaaaatctgt tttatccata tgatcgattt caatgttaaa
      901 cgaacgactc tgggattagt aactatttta tttttttctt catttctttt ttcctctttt
      961 tctcgattca gctatcttct ttcatttgga tattgattat ttcttctgtt gattactatc
     1021 cttctggaat tctagacgct gaagtaattc acgttttctt tcttctagag atcataagta
     1081 acgaatgaac gaacttcaaa taattttttt attgtaattc atatttgtat tgttcgatga
     1141 ttcttaatac gatatgacac aataaataaa aataggcatg gctatttaca acaatagtgc
     1201 tatagtagaa aacaaatgaa taaggttact taaagaggtg aatacgatga aactgatcaa
     1261 actatgggaa gcagatttaa agaaagctta tcagctacaa actagctttg gaccaaatga
     1321 gagtggattt atcaacaatg cttatggcta tacatttgct gaatttttag attatgtaaa
     1381 aaaatgcgaa aaaacgtctt taggaatcga tcttcctgag agttacgttc ccgcaaccgt
     1441 ctttatttta gaaagtgatg aacatgagta tgtaggaatt tttaatttgc gccataaact
     1501 gaacgaaaca ttaagaaacg gcgctggcca tattggttac ggcatctccc ctaattatcg
     1561 taaaaaagga tatgccacac aaggattagc attagtacta caagaggcaa aaaaaatcgg
     1621 cattgatgag gcacttttgt ctgt
//
LOCUS       DAKBVG010000301         1638 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.308, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000301 DAKBVG010000000
VERSION     DAKBVG010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.308"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            <1..214
                     /locus_tag="OUD09_002654"
     CDS             <1..214
                     /locus_tag="OUD09_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HCT9693871.1"
                     /translation="KEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHEVDMG
                     MPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            493..993
                     /locus_tag="OUD09_002655"
     CDS             493..993
                     /locus_tag="OUD09_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HCT9693872.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRVIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(1076..1627)
                     /gene="lepB"
                     /locus_tag="OUD09_002656"
     CDS             complement(1076..1627)
                     /gene="lepB"
                     /locus_tag="OUD09_002656"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCT9693873.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
BASE COUNT          492 a          284 c          290 g          572 t
ORIGIN      
        1 taaagaaata gctgataata aaaaaacaag tggtgctttg atttcgttta gtgttgaaaa
       61 cgcggatgaa gtaaaaaaat ttgctcaaat tgcaaaagaa aatggtggta ctttccacga
      121 agtagatatg ggaatgcctg aagaagaaat gttttcatta gaagtccaag atctagacgg
      181 aaatacatta gaacccgtat ggatgaagat gtaagcagat aactaatctg acgtttttta
      241 taacaaaaaa acaaaggcgg atgatacgat aagtattctc cgcctctatt tttttcattc
      301 gtacaatctc ttttttacag tttataaaag cagtcttctc accctttttt taattgttag
      361 atgccagtgt atgtaaatca ccaatgtgtc atgacacatt ggtgatttta tggtatcctt
      421 ttattattaa ctgacaaagg agagaatatt ttgaacactt tgattgcaaa agacgttttt
      481 agattgcccg aaatgaatgc aagattgttg tcgacctctg ctcttttatt agcccttgat
      541 gtcatcttat acaagatagc gattggtcct gcttattttc aaatcacatt cggattttta
      601 agtatggcgc ttatgggtta tctatatgga ccgatctggg ctggtttgct tgaaatgttg
      661 tcgaacactt taaatttcac gatatttggt tcaggaacgt ttcaagctgc ttttcttatc
      721 acggctttcc ttggtggatt tattaatggc ttatttttat atcataaaaa gataaacctg
      781 ccttccgtca tcgctgccca attattgatt atgatcccag tcagcctttt tatcaattct
      841 tggctgatgt ctgttttgtt tggtgcagat ttcaaagtga tttttctagg tagagtcatc
      901 aggaatatga tccaaatccc tattcaaata atggttgttt attcatttat caaaacttta
      961 gaggctagaa aagtgtttaa acgagctgtt tgattttacg tactaaggta gtaaactgat
     1021 tttggattaa acaataaagc gtgcagtctc aacagattgc acgctttatt gtttctcact
     1081 ggatccattt tatttcatca agtggataat agacaaatct tgctttacct agaatatcag
     1141 ttttattgat cgtgccaaac gaacgactat cttttgaaac tcttcgatta tctccaagta
     1201 caaaataatt atcttccgga agcttggatt gtcccgttag ctcttgtagt gtaaagtccg
     1261 tcgtatagga tgcatttgca tgatctgatt tcaggttttt agttagataa ggttccttaa
     1321 ctactttccc attgatcttc aattgatcat tttggtagct gacattttct cctggaagac
     1381 cgattactcg tttgatataa atcgtgccat ctgctaattg aaaaacaaca atatcaaatc
     1441 gtctgatctc cgaaaatttt tccatcacaa ccatatcacc ttgcttcaac acgttttcca
     1501 ttgaattgcc ttccactggt accgggatga ataaaaatcc ccgcaacatg aacgcaagga
     1561 atacagaaac aagtaagtat ttgaatacaa gccagaagcg attaatatag cgttgttttt
     1621 ttgtcaatct ctatcaat
//
LOCUS       DAKBVG010000302         1626 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.309, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000302 DAKBVG010000000
VERSION     DAKBVG010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.309"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            1..1589
                     /locus_tag="OUD09_002657"
                     /pseudo
     CDS             1..1589
                     /locus_tag="OUD09_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342468.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT          559 a          334 c          301 g          432 t
ORIGIN      
        1 accaatctct cgaggaaacg agtgaaccga tccgagaata tcttaatgat gaagggcttt
       61 atgacacact ccatgagtat caattaaccg gaagtgcaag aacaggcttc agtgagaatg
      121 atgaacagct tggtggacct ttatatgata atggcgaagt tgtttttaca gcgaaccaaa
      181 aaggaaatgg ctattggaag atcgaaaata aaaatcatta caccggttct ggatggatag
      241 aagatcagga cgcacccatc caaccaatcc aagaattccc tgttgacatc acgagctacc
      301 aaaccgttat agaagacaaa gaaaacatta cattgaccct aaaaaaacaa gctttcttac
      361 cttatcctta tggaaatact accttgactg atttttcatt agctagtgga caaacgcttt
      421 actatttgcc tgaaagccag cgattcattc ctgtcccgaa tgaatcgaca cgttctgcaa
      481 ctttacaaac agaaaggtta gtttttacga gagaagaatt aaaaagcagt cagttaaaag
      541 ctgatgattt agaaaatcag cagacaaatc ttcaattgcc aaaaagtttg cctaaacgta
      601 ttgaggactt ggcggtcgaa ttgaccaaaa atgaagcaac tttaattcgt aaagtcgaag
      661 ctgtctaaca gtatttaaaa acaagcggaa atttccgcta ttccaaaacg gataccgggc
      721 atactccgaa agaacacgat tatgtggatt atttcctctt tgattcaaaa gtcggctatt
      781 gtgacaactt ttcaacttct atggtcgtcc ttcttcgaac acttggtatc ccagctcgct
      841 ggacaaaagg ttttacaaat gggctcagaa caggcacaga taatcaaggg aatgaggaat
      901 acagcattct caatagtcat gctcattctt ggccagaggt atactttcct ggatttggct
      961 ggcttccatt tgaaccaacc ccttcatttg ccaatccaat gggttcacaa ccagttgctg
     1021 aaacaaccga aaccgtccag cctgctatcg aacaaccaaa tgaaagcgaa aatatggcag
     1081 aaactatttc cagtgagaga tctgaggtca ttccatcaga agaaacaaca ctcacaccag
     1141 cttataaaat cgtcttattc agccttggag tgtgtttatc cgtcttgttg ttctttctat
     1201 tgttattcag aaaatctctg cgctggacaa gttatcttat ctatcttcat ttttttgctt
     1261 ctactaatac gattgcatat caaaaacttc ttaacccttt ggaaacattg gtaccgcgca
     1321 aacccgcaga atctttgcag gaatatagcc tgcgaataga acaagaattc aagctagatc
     1381 tgcgtttcat tcgattaact actcaatatg aagcagaaat atatggacga caagcagatc
     1441 cggtaaaatt cgataaaaaa acctatttag aaataacgca tataataaga aaaacggtat
     1501 acaaaaaaat tctgacaaaa gggagccgct tcaaaaaaca agataaaatt tctggagaat
     1561 ctacttctcg aatgccgaga accagctgat tgagtatcga ttactcgaaa aaaaggttgt
     1621 gacacg
//
LOCUS       DAKBVG010000303         1623 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.310, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000303 DAKBVG010000000
VERSION     DAKBVG010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.310"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(<1..300)
                     /locus_tag="OUD09_002658"
     CDS             complement(<1..300)
                     /locus_tag="OUD09_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HCT9693874.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDN"
     gene            465..>1623
                     /locus_tag="OUD09_002659"
     CDS             465..>1623
                     /locus_tag="OUD09_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HCT9693875.1"
                     /translation="MSKGKHRKKQKISISFLVILLLSVSFFSTGIIIGGQLTKIPHSQ
                     TYKAEPTQLTSNLTPDEQRLASKIDQLLQKSDYIGSIYVRKNNRVLLEKGYGYANLRV
                     KESNSPSIYYQIGSIQKAMTALLILKQIQAGKLTFDTKLAEFYPQIPGSQQITIKNML
                     YMRSGLHRTASPDKPMSDSEIIQFALHHLKLTDYDTYHYEPLNYTLLTGILIKLTNQP
                     YETLLKKEIIRPLHLEHTDFYDNVKNSPQHAVSYQMSATDDYSKALIEPETDIRNELG
                     TGNISMSVYDLNKFFTSVLSGGIIPKELLFSLWQNNGDKHPYSGGVYSGNDYILAQGN
                     INRFHAVVAMKKDTKDAIVMESNIQSDKNIKLPATDLRNQIYELMEGVHLSN"
BASE COUNT          547 a          319 c          251 g          506 t
ORIGIN      
        1 attgtccttc tccaaaatct caccaatctg cttgttagaa gagccactcg ggatctccac
       61 ttggactaat tggtcatctg atttatctaa aggcttcaac ccgctatcta cataacgata
      121 cacggtaaat ccaagaaatc cgccaataat taataatgta agagcaatca ctaaaatgat
      181 cttcctcaca atcttatctt cttttttacg cagatttctc cgcttgtcga ttatatctgc
      241 tgtagctgcc ttactggctt cttttttatc tttacttggg tgttgtttgt ttcctgacaa
      301 aacaactcct ccttactatc atcttcgtaa ctctgtttat tatacatgaa atttctactg
      361 ccgtgaatta ttttaccaaa tattatgtca aaattgtaag tggcaaatac tctttttgag
      421 gattacaatg aaaaatagtt aaaaatggat agaaaggaat taacatgtca aaaggaaaac
      481 atcgaaaaaa acaaaaaatt tctatttctt ttcttgtaat tttattattg tctgtcagct
      541 tcttttcaac aggcatcatt ataggaggtc aattaacaaa aatacctcat tcccaaactt
      601 ataaagcgga gccaacacaa cttacttcca atttaacgcc cgatgagcag aggcttgcta
      661 gcaaaattga tcaattacta caaaaatcag attatatcgg ttcgatttat gttcgcaaaa
      721 ataatcgcgt actattagaa aaaggatatg gatatgcaaa tctgcgcgta aaagaatcga
      781 attcaccttc tatttactat caaatcggtt cgattcaaaa agcaatgact gctttactga
      841 tactaaaaca aatccaagct ggtaagttaa cttttgacac aaagttagca gaattttatc
      901 ctcagattcc tggaagtcag caaatcacaa tcaaaaatat gctttatatg cgctctggtc
      961 ttcatcgaac tgcttctccc gacaaaccta tgtcggatag tgaaatcatt cagttcgccc
     1021 ttcatcattt gaaacttact gattatgata cgtatcatta tgaaccattg aattatacat
     1081 tgcttacagg gatattgatc aaactaacga atcagcctta tgaaactctt ctaaaaaaag
     1141 aaatcattcg tccactccat ctagaacata cagattttta tgacaatgta aaaaattcgc
     1201 ctcaacatgc tgtatcttac cagatgtctg caactgacga ttattcaaaa gcattgatcg
     1261 agcctgaaac cgacatccga aatgaattag gtacaggaaa cataagtatg tctgtttatg
     1321 acttgaacaa attctttacc agtgttcttt ctggcggtat cataccaaaa gaactacttt
     1381 tctcattgtg gcaaaataat ggtgataaac atccttacag cggcggtgtt tacagtggaa
     1441 acgattatat ccttgctcag gggaatatca atcgattcca tgcagtagtg gcgatgaaaa
     1501 aagacactaa agatgcaatc gtcatggaaa gtaatatcca atcggataag aacatcaaat
     1561 tgccggccac tgatttaaga aatcagattt atgaactgat ggaaggtgtt cacttatcaa
     1621 att
//
LOCUS       DAKBVG010000304         1619 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.311, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000304 DAKBVG010000000
VERSION     DAKBVG010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.311"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            287..694
                     /locus_tag="OUD09_002660"
     CDS             287..694
                     /locus_tag="OUD09_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693876.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            708..1109
                     /locus_tag="OUD09_002661"
     CDS             708..1109
                     /locus_tag="OUD09_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693877.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1111..1482
                     /locus_tag="OUD09_002662"
     CDS             1111..1482
                     /locus_tag="OUD09_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693878.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
BASE COUNT          606 a          208 c          282 g          523 t
ORIGIN      
        1 tgaggggaag tacatatagt tgttgatata gttgctaata ttgttagcta atttgttagt
       61 aaaactgtat acgaatttag atagtaaaaa aacagtttgt tccttatata gaggcacttt
      121 tactactgat taaatgtcag atatgcttac aaatatactt ataaatatgc ttacaaaatt
      181 agctaatatt tccgttgaca aagctttgtt actattgtta aactaaattg actagcaaaa
      241 gtgaaacata aatttattaa ataaatgtaa aaattaggag aaatcaatgt cttatagagt
      301 acaattaata atttcagaag atgtagaaag tcagcaattt ggcactaatg ttattaataa
      361 agtaattaat ccagctcttt ctattaatgc gcctttaatt cctactgctt tatcattttc
      421 tgtaacagct gtggtgtctg agatagagga tacagaaaag ataaaaatag tagaaattga
      481 agttttaaac aagaatgaaa aacaaatttt ttctacgggt gaagtatcgg taagcttgcc
      541 tcctcaagtt aacgatataa actttaatat aaacgccaga aatgtcttgg ttgaagaagc
      601 aggagagcat tatgctgttg ttaaatttaa tggaactgag attggaaggc aaatctttga
      661 tatcaaggtc aacaaaccag tggaaaaaaa ttaaggagac gatacggatg cttgatatag
      721 taccatcaaa tacacatcgg agtggtagct taataaattt tgctgcgatt tttgcttgcg
      781 catcggtaat tctagcatct ccagttgttg tggatgcaag ttctactccg aaaactaaaa
      841 atgataatca acttgtaatt actacaaaaa atgagattaa cacaatttca agtaatgatg
      901 gcaaaatttt tgatgtgata aatacggttt taaaaagaaa agctgaaatc aatataaaat
      961 atgatgaaga tttaaacttg tatttctttg ttatcaaaac aacatctgaa ttgttttcct
     1021 cagattacga tgttttggat actctcgata atgtattatc tgactataaa tatatgggga
     1081 aatctgtagt agcaactttg ggggaataaa atgtttgact ttaatgaata tttatctatt
     1141 tccaaacaaa ttgaatcaga tgataaatat aattctaaag aatcttatag aagaactgct
     1201 gtttcaagag catactacag tgcttttaaa aaatctgatg agtatttgaa agaaaattat
     1261 gatattattt ataatggaag tagcggaaaa ggaagccacc aaaccgtttg gaatttgttt
     1321 tcaacagtta aggaattgaa taccttagga atacataata gtgggtatag aatgttagag
     1381 aaaagaaaat gtgctgacta tattcctaat gaaaccatta cgaaaacaga tatggcgctg
     1441 atgaatcgag aagcagaaaa aataataaac aaactcacct aatgaatttc aagcgagttt
     1501 gtttgttatt tttttattta atctttgtat ctttctggat caacgaaagt atactttata
     1561 tagtcataac gccgatgatc gctacgtgcg tccggcacgt cagtcacgat atcaaacaa
//
LOCUS       DAKBVG010000305         1569 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.312, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000305 DAKBVG010000000
VERSION     DAKBVG010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.312"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(<1..210)
                     /locus_tag="OUD09_002663"
     CDS             complement(<1..210)
                     /locus_tag="OUD09_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCT9693879.1"
                     /translation="MDTITPTNFKENIGKVLQEVVHSNSEIEITRDSKNGLNDGVIMI
                     SKRKWELLQEELYLQRTGTWAYVSDL"
     gene            complement(309..659)
                     /locus_tag="OUD09_002664"
     CDS             complement(309..659)
                     /locus_tag="OUD09_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693880.1"
                     /translation="MIFENESEEIGRVAEENKVDPLETLVINKKEFEQLFLDFFIQYD
                     MIYDFSSSLDLFELEAEIIENSREKNGKIIISVEKFNELFSQTFEIDPDYYQPQEVFR
                     EFRNKIIGFSQTTK"
     gene            complement(700..1194)
                     /locus_tag="OUD09_002665"
     CDS             complement(700..1194)
                     /locus_tag="OUD09_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693881.1"
                     /translation="MFKVYFPFQWLVKGILLVLLGLILFGSYLTIKARHFPEEKPIKI
                     VTKDLKIGETYQADGVELTFKNIEVTEKRNEFAQHDRVLQVTYTLINDTENPIRPQQW
                     SIENKDGEELYEYAMILDNPEYIESGKEETVNYYYELPIERQDYQIVVRRANGYFVGL
                     PFTL"
     gene            complement(1187..1501)
                     /locus_tag="OUD09_002666"
     CDS             complement(1187..1501)
                     /locus_tag="OUD09_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693882.1"
                     /translation="MNYPYFKVSASEETKEIFNNFYNQNKGVFGSKANMFRVMVSNLP
                     VLASPSNNKFNDPESIKFEQKISELESMISNEVIEKLDDIDQKLSYSLKNKYKTEEKK
                     DV"
BASE COUNT          476 a          289 c          197 g          607 t
ORIGIN      
        1 taaatcagat acataagccc atgtacccgt tcgttgtaaa taaagttctt cttgtaacaa
       61 ctcccatttt ctcttagaaa tcataatgac gccatcgttc aatccatttt tagaatccct
      121 agtaatttct atttcagaat ttgaatgaac tacttcttgc aacactttac ctatattttc
      181 tttgaagttt gttggagtaa tagtatccaa aatgaatacc tctcttctct atacctctat
      241 tatgaagtgc ccataaaata aagcaagcgt tgaggaataa ttctatcaac acttgcttta
      301 ttaagctttc attttgtagt ttgactaaaa ccaataatct tatttctaaa ctctcgaaaa
      361 acttcttgtg gttgataata gtctggatct atttcaaatg tttgtgagaa taattcattg
      421 aatttttcta ctgaaataat gattttacca ttcttctctc gactgttttc tattatttct
      481 gcttctaatt caaataaatc tagtgacgaa ctaaaatcat aaatcatgtc atattgaata
      541 aaaaaatcaa gaaaaagttg ctcaaactct ttcttattta ttactagtgt ttcaagcgga
      601 tcaactttat tttcttcagc cactcttcca atttcctcac tttcattttc aaaaatcaca
      661 aaaatgatat taacttaaaa gccactaaca aaataagact tacaaagtaa agggtaatcc
      721 aacaaaataa ccattcgctc tacgtactac gatttggtag tcctgccttt caatcggtaa
      781 ttcataatag taatttactg tttcttcttt gccactttca atatactctg gattatccaa
      841 tatcatagca tactcataaa gttcttcacc atccttgttt tcaatcgacc attgttgtgg
      901 acgtatcgga ttttctgtat catttataag tgtataggtc acttgtaata cacgatcatg
      961 ttgtgcaaat tcatttcttt tttcggtcac ttcaatattt ttaaatgtta gttcaacccc
     1021 atctgcctga taagtttcac ctatttttaa atcttttgtt acaattttta ttggcttttc
     1081 ttctggaaaa tgtctggctt taattgttag ataacttcca aaaagaatca atcctagtag
     1141 cactaaaaga attcctttaa cgagccactg aaacgggaag taaactttaa acatcttttt
     1201 tctcctccgt tttgtatttg ttttttagag aatacgataa cttttgatca atgtcatcca
     1261 gcttttcaat tacttcatta cttatcattg attctagttc tgaaattttt tgttcaaatt
     1321 taatgctttc aggatcatta aatttattat ttgatggact agctaacact ggtaaattcg
     1381 aaaccataac acgaaacata tttgcctttg aaccaaaaac tcctttgttt tggttgtaaa
     1441 aattgttgaa tatttcttta gtttcttctg aagccgagac tttaaaatat ggatagttca
     1501 tttttttctt ccacctttct ttttttggga gggagtctgt agggagcttg ctccctcaga
     1561 gatttaccc
//
LOCUS       DAKBVG010000306         1553 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.313, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000306 DAKBVG010000000
VERSION     DAKBVG010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.313"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            <1..121
                     /locus_tag="OUD09_002667"
     CDS             <1..121
                     /locus_tag="OUD09_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCT9693883.1"
                     /translation="ITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFLSILI"
     gene            132..1547
                     /locus_tag="OUD09_002668"
     CDS             132..1547
                     /locus_tag="OUD09_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCT9693884.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
BASE COUNT          598 a          184 c          270 g          501 t
ORIGIN      
        1 aataacaaag gggatagaat ctcctgtgaa aagaagttct tactataaaa caaacatgag
       61 cttattagac tatatcatcc tgttttccgt tactattttt ttcttaagta ttttgatttg
      121 aggtgaaaga attgtttgaa tttaacaatg tttctttttc atataaggat gttaaggaac
      181 aagcgcttgt tgaccttaat ttttcaataa aaaaaggtag tcttacattg attactggtt
      241 cttctgggag tgggaaatcc acactattaa agttaatgaa tggacttatt ccaagacttt
      301 atgacggaaa attggttggg gaaattttat ttgaaaataa ctcgctacaa tccttaacac
      361 aagaaagtat atcaaaaaat attggctacg taagtcaaga tcctagagga caattcttta
      421 caacaaatac aacgagtgag ttagtatttt cgatggaaaa ctttggattt acaaaagagc
      481 atatggataa aagaatcaat gaattagctg aattattaga tctttcaaat atattagata
      541 aaaacatttt ttcgctatca agtggtgaac ggcagaaaat ttctattgga tgtagtttat
      601 cgctaaactc taaattaatt atattggatg aaccgtcttc aaatttggac ttcaaagcga
      661 ctaagaaact tgctgactta ctttgtgatt taaaaaataa aggatttact attgttatag
      721 ctgaacatcg tttttactat ttgaaagaat taattgatca agttctattt gttaaaaata
      781 gaactgtaaa aactacaact attcagttgt taaaaactga aaacaataac gcattaagga
      841 aatttgacat ttttaatagt tcgattgaag ttccctatat cgaaaaggta agtgcagatg
      901 tattaaaagt tgaagaaatt acttatgaaa atattctaaa agacataaat tttgatgtca
      961 aagttggaga tgttattggt ttggtaggga aaaacggtgt ggggaaaaca accttactaa
     1021 gaatactcat gaagattatt cgccctacca aaggaaagat aatcgaaaat aaaaaaaatt
     1081 tgtcatcgcc gtttttagta atgcaagaaa tggattacca atttttcaca gaatcagtga
     1141 ggagcgagtt agccctgggg aatgaaatag ttagtaaaga tcagcaagaa aaaattttaa
     1201 aaaaaatgga actttataaa atgaaagatc aattaccgtt tgatttatcc ggtggagaaa
     1261 aacaaagatt attggtttct atagcttcat tgtcaaaaac taatttattt ctatttgatg
     1321 aacctacaag tgggttggat tatattaata tggaaaggat tgctgacctt attaaagact
     1381 taaaagaaaa gggtgcagta gttatagcca ctcatgatcc ggaatttctt tataaaacat
     1441 gcaatagaat tatttattta aaaaatggtg gaataaaaaa agatataaaa cttagagtag
     1501 aagattcaac tttgatagaa cagattttta atgacataac gaaatagaat tga
//
LOCUS       DAKBVG010000307         1553 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.314, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000307 DAKBVG010000000
VERSION     DAKBVG010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.314"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(112..996)
                     /locus_tag="OUD09_002669"
     CDS             complement(112..996)
                     /locus_tag="OUD09_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HCT9693885.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPEAINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSILLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(1091..1528)
                     /locus_tag="OUD09_002670"
     CDS             complement(1091..1528)
                     /locus_tag="OUD09_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693886.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWVFYLVKK"
BASE COUNT          491 a          327 c          234 g          501 t
ORIGIN      
        1 ggtgaaaatt tcttttttgt acaaaaaaag gctgtgacaa aagtcttgtc acagtccctt
       61 attccgaaca accgatgtcc tcctgacaac tcttcggtca caacctccat tttaacaata
      121 aacctgtaaa tgtgagggta acactttgat tgtcacaggt aatttatccc cttcatctcc
      181 atcaacattc gtcgtcaatt cttcctcatc agctaatgac acaactaatt gctcacaagt
      241 aagataggct agatttttcg tagattcatc tactcctttt aataggtctg gcaatgtttt
      301 aagcgtatcg atcaaagaag aatcttttaa ataaaccaaa tggagcttac catcatccac
      361 ttttgcattt ggaagtaatg tttcaaaacc gcctactgag ttagttagtc ctatcagcaa
      421 gatactgctc tcaatttctt ccttctttcc atcaacttct acgtgaaacg agtaggattg
      481 tgtggaagct aactgtttga ttcctgacat gaaataagct agttttccaa atttcgtctt
      541 cttctccgca tctacatcat tgattgcttc agggatagag ccaattgcga cgacattcat
      601 aaaatagtcg tcatttattt ttccaatatc caatggcttg actgattcaa tcgaaaaatg
      661 attgatcgct tcctcaggtt ctagcggtat ccctaatgct cgagccaagt cattcaccgt
      721 acccaatggg aaaaatccga aatttggccg atgttcttgt tcagcgattc cactgattcc
      781 ttcgttgact gttccatctc cgcccatgac aaacacacta tgataccctt ccgtagccgc
      841 ttcgcgagta aagttttttg catcccctgc tttttttgta tgcaagacga ccacttcatc
      901 aaacaccgat tccaattttg ctatcgctaa ttgctcaaat tcctttgctt gttcaccgcc
      961 tgaactgggg tttaccacta ataaaacttt tttcatcact ttctttccct tctgattgat
     1021 ttctttaata taacgatagc ataaagaaaa aaattaggct gtgatgaagt actgttcatg
     1081 aaacattcat ttattttttg acaagataaa atacccatac tatgaaactg aggaaaatta
     1141 aaacaatgat ataggaaata aaagaaaatg ataggcattg tatacctaat aaagtaaaaa
     1201 tcttaggtgg cagataaaaa cacaaaaata gcaagagaat gtaatagcgt ttcttccact
     1261 ttttgttatc agaccttata gcgatcagag gaataccaaa tgcataactg aaaaactctt
     1321 gatgtactgt taatatgtca cgaccttgaa taataaatgc aacaatcccc gctataatta
     1381 gtgtgcctaa taccaaaacg gaaaattgat gaatatattt tatatacaaa tgatccattg
     1441 tacttcctgc ctttttattt gtgagaaaaa caaatccgta tacgtgaata acttctagaa
     1501 ataaaagaaa tttgcaaaac cactccattt atctacttct acgtgcattt ttt
//
LOCUS       DAKBVG010000308         1548 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.315, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000308 DAKBVG010000000
VERSION     DAKBVG010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.315"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            237..>1548
                     /locus_tag="OUD09_002671"
     CDS             237..>1548
                     /locus_tag="OUD09_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCT9693887.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIE"
BASE COUNT          568 a          213 c          277 g          490 t
ORIGIN      
        1 ggttgtttac gcaaatggcg caaatcttgt acattttaac acaataaaac aaaaaatata
       61 gaatatttcc tcataaattt ttccattttt tgcaatattc tcaaaaaaaa gataaaaaat
      121 gagaataact gatgaaaaaa caagcataat ccatcataaa atcagtatag gtgaaaagat
      181 gaactttcat ctgcatatca agtaattggt atgaggcatt ttttgaggtg taggatatga
      241 agttggatta ccttatagaa gaggaagata gagaaaaact gctgctagct agatatttag
      301 aatttgagaa aaagaattat gtagtgctgc ctaagttata tgaaacatta ggtttgtcta
      361 aatttaaaac aagaaattat atcatagaat taaataaaga atttactgaa ttcaaaacaa
      421 agccagagat ccaaatttca gaagaagcag agatccatgt cgaacgtctg aatttgacgg
      481 atatcaaaaa tttaagaaga atctactttc aacgatcaaa aattgctcga ctgttaacgg
      541 agatcgtgga ttcaaaagta acgatcgaaa aatttgcaga gaatcatttt ttaagcatat
      601 caagagccta tgtaaaaaga agagaattag tctcttttct tgaacagcat caaataaagt
      661 taaagaaaaa catattagtt ggggaagaaa taaacatacg gaacgtatta tttggggtat
      721 atttcggggt ctttaatggt ttttctatgc catttagaga aagcgtcatg aatgaagcaa
      781 agagtatcgt gcaatatttt gaatatctgt tcaatttaaa actgtctgca acaaaacgaa
      841 tcaaattcaa tttgctaacc actattagtt tgttgagggc ctatgggaat caaccaatcg
      901 gaaaacgttt cttttcagat gaatacttta agagctcaga aggaatgaag atcatcaagg
      961 atttatcaca tatttctttt cttcatatcc ctaaagagca gatcattgat gagatttctt
     1021 atatcatgtt gtttttgatc acggaagaag aatttgaatt agatggcgca tttggacttg
     1081 cggaagctca ttttcatgat atccacatga ttagtcaaaa aatgtcagtg gaattgattg
     1141 atcaattacc attttcgatt gaaatctctg aagaagaaag actatatata cgaaaagaac
     1201 ttcgtatggg tatgatgcat gtcaatcgaa aaaattggat attcgacttt gagatgacta
     1261 gctttacgtc taaaaagcag ctacagtttt tcttcgagac gtatccaata ttcagtacag
     1321 caatccaaaa ggtcatacaa gagaataaac gctatttctt gggaaaagat gaacgtttca
     1381 tgattcgctt attttatgat tatctctttt tattggtcgg tatttgtccg gtatcatatt
     1441 ttgaagcacc gattcatgtc tgtattgatt tttcgcaagg atctgcttat accgattaca
     1501 ttatttctca agtgaaagga tttaaaaatt acaatattgt gattgaga
//
LOCUS       DAKBVG010000309         1533 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.316, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000309 DAKBVG010000000
VERSION     DAKBVG010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.316"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            95..505
                     /gene="spx"
                     /locus_tag="OUD09_002672"
     CDS             95..505
                     /gene="spx"
                     /locus_tag="OUD09_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HCT9693888.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     TLAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            519..875
                     /locus_tag="OUD09_002673"
     CDS             519..875
                     /locus_tag="OUD09_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCT9693889.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVEKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          564 a          227 c          281 g          461 t
ORIGIN      
        1 tattttgatt tttttttata ttttattagc taataattgg tattttaaaa gaaattactg
       61 tagaattgga ttataattaa aaaggagtga gaaaatgata aaagtatata cttcgcctag
      121 ctgtacttcc tgtagaaaag caagagcatg gttgcaagcg aatcaacttg aatttgaaga
      181 gaagaatatt tttgccgaac cgttaacaga aagtgagatc aaaaagacct tggcagcgac
      241 tgaaggagga gttcaagata ttatttccac tcggtcgaaa atttacgaaa agctgggcat
      301 agattttaac gaattaactt taaagcaaat gatcgtctta ttcaaagaat atccttcatt
      361 attgagacga cctattttga ttgatgaaaa aagaatacta atcggattta atgaagatga
      421 gatccggtca tttgtccctc gtgaaattcg atgtgttgcc agaaatctga tctatttaca
      481 aaacaataac atcttaaatt catagggagg gagccttcgt ggaagtgaaa gtctatggat
      541 cagcgcactg tgtaacaagt ctgaaggcga aacaatggtt agaacaccat caattgcctt
      601 atcaatttgt tgacttagaa gaacgcgatc taagtacaga agaagcagaa gaattatgca
      661 gttttgaaga agtgcaagca gaacagctat ttgcgacttg gtctgaggct tttcacaaca
      721 ttgcgattga cctttcctgt gtagaaaaaa aacaactgat ttttttatgc acacaacata
      781 ccaacctcct cagacgtcct ttgattatta tcgatgaagt tctatttatt ggatacaatg
      841 agcagttgat ggaacagctt atcttaaata aatagacttt aactgtttta atcaagtcac
      901 tgagcattcc atacagttgt aacagtcgaa cacttagcat caagcgtgtt atgtgttctt
      961 tttttgactt tatttttcta ctatagagac atgctcaatt tatgtggtca aaattcaaat
     1021 ctaggtgggg atatagagtt aaaaatatat tgacatgtgt aaatttacat gatatattca
     1081 tttctgttgt tttaagtagc aaagcaataa aatagtattt taaaaaacat ataaaatagt
     1141 tgttgacatg ttagaaatga catgataaga taactgagtc gttaaaaaag gaccgcttgc
     1201 gaagcttgat tctatcgaaa aaacttcaaa aaagttcttg acaaataaca aacgacaagt
     1261 tatgataaat gagttgctgt aagatttcga aaaacgaaat caaaaaaact tttaaaaaag
     1321 agttgacaat tacttgtctc tctgatatga tataaaagtt gctgcggcag ccaaaaacga
     1381 atcgggaaac actcctgatg aagtagacct ttgaaaaatg aacaaagtaa gacaaaccaa
     1441 atgtgtaggg cgtcttgatt caattcaaga caacaaacat ttttaacaag caagcaatat
     1501 gctagcaaac aaattgagct taacaatcgc aag
//
LOCUS       DAKBVG010000310         1530 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.317, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000310 DAKBVG010000000
VERSION     DAKBVG010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.317"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            93..533
                     /locus_tag="OUD09_002674"
     CDS             93..533
                     /locus_tag="OUD09_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCT9693890.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            543..1505
                     /locus_tag="OUD09_002675"
     CDS             543..1505
                     /locus_tag="OUD09_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HCT9693891.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
BASE COUNT          526 a          255 c          339 g          410 t
ORIGIN      
        1 atgatctttt aaggttgcat tttgaaaaaa gttaagatat agtttgaaca tcaaaagatt
       61 tgataatcaa actaattagt ggacggtgga acatggaacc agatttaaaa acagtaaatg
      121 attatcttgt tagtgtcttc aatgatattt tgacaatcga agagtctgag ctgaaaaaat
      181 ctcaattcaa agatctgtca atcacagaga tgcacacgat cgaggcaatc gggatgtaca
      241 agaaaaaaac aacttcagaa gtagccaaag aactatcaat tactgttggg accttgacca
      301 ctgctattaa caagttagta aaaaaaggct atgtcgaacg tatccgcagt gaagatgacc
      361 gccgcgttgt aaagttggga ctaacgaaaa aaggaaagtt actatttcgg gtccatcagc
      421 attttcatcg tgaaatggtc aagaacatct tagacggaat ggctacggag gaacagcatg
      481 ccttgctgga tgcactgaaa aacttgcacg acttcttgca agactataag tgaggatatt
      541 taatggaaca atttggaatg attactgcaa cagccagcgt ccttcctgaa aagattatta
      601 ctaataatga tctttccaag atgatggata cctctgatga atggatttcg tcaaggacgg
      661 gtattcgtga acgaagatgt gtaacaaatc aggagacttc tgatttatgc attttagtgg
      721 cggaaaaatt gataaaacag aaaaatctaa accctaaaga tttggatttt attttagtag
      781 caacaatgtc gcccgattac acgacgccat ctgtagcagc acaagtacaa gggaaattgg
      841 gagcaactca tgctatcgct tttgatatca gtgctgcgtg ctcaggattt gtttatgcgt
      901 taagcatggc tgaaaaaatg atccgctgcg gctcaactaa gggactagtg attggtggtg
      961 aaacactatc taagctaatc gattggtctg atcgtacgac tgctgtgttg tttggcgatg
     1021 gagcaggtgg gattctctta gaagcgaatc cacagaaaaa attgctgaaa gaaaaattag
     1081 cagctgacgg taccagaagt agctcattga ccgctggcta ccagcaaaat aaaaatccgt
     1141 tctattcaga agattcagaa ggttcgtatt atttgcaaat gactggcaga gatatttttg
     1201 attttgccgt acgagacgtt gcaaacaata tccaagaagt catgaaagac aaaccagttg
     1261 attatctgct tttgcatcag gcaaacctca gaattattga gaaaattgcc cgaaaagtaa
     1321 agatgccgca agaaaagttc ctgaccaata tggataaata tgggaataca tctgctgcaa
     1381 gcatccctat cttattggat gaagcagttt ctagtggaaa gatcaccttg ggaaaacagc
     1441 agaaagtaat atttacaggc tatggcggag gtttaacatg gggaagtatt ctcatggagt
     1501 tgtagactgt taaattataa aaataaaaag
//
LOCUS       DAKBVG010000311         1519 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.318, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000311 DAKBVG010000000
VERSION     DAKBVG010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.318"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            <1..1469
                     /locus_tag="OUD09_002676"
     CDS             <1..1469
                     /locus_tag="OUD09_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCT9693892.1"
                     /translation="LTTEIQLLNEKVNYLTNKLFGRSKETLFEETNGQLNLFSDEEIS
                     VSVPEAAATIIPVKGHQRVVGTKADKIKHLPITEKEHLLPLEEQFCEHCGSQMKDIGR
                     TKVREEIRFHQAMLDCLTHYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVA
                     ETIRMKFGQKVPAYRQENYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQ
                     EEILHGDETSYRVIESAKTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFT
                     GYLHCDGYSGYNAVESVRLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVL
                     EKKWKDFSPEKRLSCRQQELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEP
                     IFEDGRLELTNNRAERNIKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPV
                     KYLEFLFDKIPNLPVLSAETLDQLLPWNKDVQQHFSRN"
BASE COUNT          509 a          273 c          317 g          420 t
ORIGIN      
        1 atcttacaac tgaaatccaa ctattgaatg aaaaggtcaa ttatttaacc aacaaattat
       61 ttggacgctc taaagagact ctctttgagg aaacgaatgg gcaattgaat ctttttagtg
      121 atgaagaaat ttctgtaagt gttccagaag cagctgccac cattattcca gtaaaaggac
      181 atcaacgagt tgtaggaacg aaagcagaca aaatcaaaca cttacctata acagagaaag
      241 aacatctcct tcctttggaa gaacaatttt gtgaacattg cggttcacag atgaaagata
      301 tcggacggac aaaagtacga gaagaaattc gtttccatca ggctatgttg gattgtctca
      361 cccattatca acatacctat tgttgtaaaa gctgcgaaaa agaaggactt tcttctttta
      421 aaaaagcgat cgttcctaaa cctttgattt ctaatagctt aggttccaat agtttagtgg
      481 cggaaacgat ccggatgaaa tttggtcaga aagttcctgc ctatcggcaa gagaactatt
      541 ggaaacaaac acatgggttg gatatttcga gagataatat caccaattgg catattaaag
      601 cagtgcaaaa cgcgttagac cctttaggag aacgattaag agtctatctt aatcaagaag
      661 agattctaca cggggatgaa acaagttatc gtgtgattga aagtgccaaa acagatacct
      721 attactggca gttttgtaca ggaaaagaca gtcagcatcc catcgtttat tatcaccacg
      781 acgaaagtcg tgctggagat gttccgaaaa cttttctcaa ggagtttaca gggtatttac
      841 actgtgacgg ctatagcggg tataatgccg tggaaagtgt acgacttgtc tattgtttcg
      901 cgcatgttcg gcggaaattt ttcgaagcaa ttccaaaagg aaagaaaaac acggatattc
      961 ccgcagctca agcagtcaaa caattggata aatggtttgt cctagaaaag aaatggaaag
     1021 acttttctcc tgagaagcga ttaagctgtc ggcaacaaga actgcgtcca ttatttattg
     1081 ctttttatga gtggatggca acgatagatc ctgttgcaaa atccaagttg gatgcagccg
     1141 ttcaatatgc gtgtaaacta cgaagtggtt ttgaacctat ttttgaagat ggtcgtttag
     1201 aactcacaaa taatcgtgcg gagcgaaata tcaaagagct ggtgatagga cggaaaaatt
     1261 ggttgcattc cacaagtctt gaaggagcac gaacttcagg aatcattctg agtgtatata
     1321 aaacagcaga acttaacgga ttgaatcctg taaagtatct agagtttcta tttgataaga
     1381 taccaaatct tcccgtactt tcagcagaaa ctttagatca actgttgcct tggaacaagg
     1441 atgtacaaca acatttttct cgtaactaaa aacgccaaac attcccgatt ctattctata
     1501 ggtcgggtta tttgacgta
//
LOCUS       DAKBVG010000312         1509 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.320, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000312 DAKBVG010000000
VERSION     DAKBVG010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.320"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            <1..1328
                     /locus_tag="OUD09_002677"
     rRNA            <1..1328
                     /locus_tag="OUD09_002677"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            1423..>1509
                     /gene="rrf"
                     /locus_tag="OUD09_002678"
     rRNA            1423..>1509
                     /gene="rrf"
                     /locus_tag="OUD09_002678"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          421 a          323 c          446 g          319 t
ORIGIN      
        1 gaagttcctg atgtcacact gccgagaaaa gcttctagtg agaaaacagc ggcccgtacc
       61 gcaaaccgac acaggtagtc gaggagagaa tcctaaggtg agcgagagaa ctctcgttaa
      121 ggaactcggc aaaatgaccc cgtaacttcg ggagaagggg tgctgatcat acgatcagcc
      181 gcagtgaata ggcccaagcg actgtttatc aaaaacacag gtctctgcaa aatcgtaaga
      241 tgaagtatag gggctgacgc ctgcccggtg ctggaaggtt aagaggagtg cttagcgcaa
      301 gcgaaggtac gaattgaagc cccagtaaac ggcggccgta actataacgg tcctaaggta
      361 gcgaaattcc ttgtcgggta agttccgacc cgcacgaaag gcgtaacgat ttgggcactg
      421 tctcaacgag agactcggtg aaattttagt acctgtgaag atgcaggtta cccgcgacag
      481 gacggaaaga ccccatggag ctttactgta gtttgatatt gagtgtctgt accgcatgta
      541 caggataggt aggagccgta gaaatcggaa cgctagtttc gatggaggcg ctggtgggat
      601 actacccctg cgttatggcc actctaaccc gcaccactaa tcgtggtggg agacagtgtc
      661 agatgggcag tttgactggg gcggtcgcct cctaaaaggt aacggaggcg cccaaaggtt
      721 ccctcagaat ggttggaaat cattcgaaga gtgtaaaggc agaagggagc ttgactgcga
      781 gaccaacaag tcgagcaggg acgaaagtcg ggcttagtga tccggtggtt ccgcatggaa
      841 gggccatcgc tcaacggata aaagctaccc tggggataac aggcttatct cccccaagag
      901 tccacatcga cggggaggtt tggcacctcg atgtcggctc gtcgcatcct ggggctgtag
      961 tcggtcccaa gggttgggct gttcgcccat taaagcggca cgcgagctgg gttcagaacg
     1021 tcgtgagaca gttcggtccc tatccgtcgc gggcgttgga aatttgagag gagctgtcct
     1081 tagtacgaga ggaccgggat ggacttaccg ctggtgtacc agttgttctg ccaagggcat
     1141 tgctgggtag ctatgtaggg aagggataaa cgctgaaagc atctaagtgt gaagcccacc
     1201 tcaagatgag atttcccatt tctttaagaa agtaagatcc ctgagagatg atcaggtaga
     1261 taggtcagga gtggaagtac agtgatgtat ggagcggact gatactaatc gatcgaggac
     1321 ttaaccaata ggttaaaaga aaagaactcg gaaaaaaact tatgtgactt caaatccagt
     1381 tttgagtgaa caaaatttac tcaataaaag ataacaccac agtgtggtgg cgatagcgag
     1441 aaggatacac ctgtaaccat gccgaacaca gaagttaagc ttcttagcgc cgattgtagt
     1501 gaggggttg
//
LOCUS       DAKBVG010000313         1495 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.321, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000313 DAKBVG010000000
VERSION     DAKBVG010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.321"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            27..467
                     /locus_tag="OUD09_002679"
     CDS             27..467
                     /locus_tag="OUD09_002679"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCT9693893.1"
                     /translation="MVPRIFGLTPYIIEDKEMSPYYAQGNLIYVRPKNTNEILVGDII
                     TYYENSGKKISTRRVIAVENNHEDFYTKADTKTYIEPGVVKKRNIIGSPIFQIPYIGL
                     VLSKTAFAWIQGLFFTIAFCLTGITAWNTFVELKKKRRRTQKFL"
     gene            595..1278
                     /locus_tag="OUD09_002680"
     CDS             595..1278
                     /locus_tag="OUD09_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HCT9693894.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
BASE COUNT          494 a          242 c          318 g          441 t
ORIGIN      
        1 ttttaattct tattcttctc gtcgtcttgg ttccccgaat ttttggtttg actccttata
       61 ttattgagga caaagaaatg agtccatatt atgcacaagg aaatctgata tatgtacgac
      121 cgaaaaatac aaatgagatt ttagtcggag atattattac atattatgaa aattcaggaa
      181 aaaaaatctc gacgcgtcgc gtaatagccg ttgaaaacaa ccacgaagat ttttatacaa
      241 aagctgatac aaagacttat attgagcctg gagtagtaaa aaagagaaat attatcggta
      301 gtcccatctt tcagattcca tatattggac tagtcctttc aaaaacggca tttgcatgga
      361 ttcaggggtt attctttacg attgcttttt gcctaacagg aatcacagca tggaatacct
      421 ttgtagagtt gaaaaagaag cggagacgaa cacagaaatt tttgtaagaa tgttaaaaac
      481 ataaatgtat tccagagatc caacgactct tctgaacatg aaacacgaga aaacactcat
      541 gcagcttgta gatttagtca gttgttcgta taatgtgtga agaggaggag tagtatgcgc
      601 aatgaaatga tgcatcgtcc tgtagtcaaa gaagaactat tggattttat gcgtacccag
      661 caaaaaaaat taccgggtga gctaggcaaa ctggaagaag aagctcatgt agcagaagta
      721 ccagttattc cccatgaaac agttgtattt ttgaaatttt tacttggaca gcttcagcct
      781 gaacgaatct tggagatcgg agcagctatc ggtttttcat caagtgtcat ggccacgaca
      841 atcggagaaa acgctcatgt tacgacaatt gatcgtttcg atgtcatgat cgagaaagct
      901 aaaaaaaact atgaacgtct aggactgaca gataaagtga ctttgcttga aggtcaagca
      961 gcagatatct tgccggagtt gtctggtccg tatgacttta tttttatgga tagtgcaaaa
     1021 tcaaaatata ttgaattttt acctgaatgc ttacgtttgt taagaaaagg tggcgttttg
     1081 atggtagatg acatcttcca aggaggaacg atcttgctcc cagatgaaga gattccccgt
     1141 ggcaaacgag ctatccatcg taaattaaat gaatttcttc gtgttgtgat ggatcatccc
     1201 gatttgacat cgacaatctt gccattaggt gatggtgtga tcttaatgac aaaggaatca
     1261 gaaacaatcg agttgtgata catgattatg gcagcctgta gataatcgat tgaatcgagg
     1321 gctcaattcg aacttgatgt aaaagtttct atatttgtaa gtcaattaaa ggatacagga
     1381 aatctaaata aacaggagta gcatgaagtc aatgtagctc ctgttttcaa ttgataacct
     1441 tagaacagct gaaggagaat gtctcgtttt aaaagattga tttagtagtt gggcc
//
LOCUS       DAKBVG010000314         1458 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.322, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000314 DAKBVG010000000
VERSION     DAKBVG010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.322"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            195..1364
                     /locus_tag="OUD09_002681"
     CDS             195..1364
                     /locus_tag="OUD09_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HCT9693895.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
BASE COUNT          537 a          214 c          235 g          472 t
ORIGIN      
        1 gagtttggac ctagcaaaca atattaatga ggtcattaaa tcttattatc tttagtgata
       61 taacgaaata tctacacaga caaaatgata gcgtaaaata ttttgtgtaa atagatattt
      121 agggtagaaa aaaagaaagt ccattctgta aaattaaagt taccacaacc gaaattttat
      181 aggaggactt tctcatgccc aattttacta cagaaattat ggaaacacta atcaataaag
      241 gtgatttaga tgaattattt cgatgtcatt tagaattagc tgttaattca ttattgcagg
      301 ctgaattaac agcttttctt gattatgaaa aatatgatcg tgctgggttc aattcaggta
      361 attcccgtaa tgggaattat tcacgttcat tcagaacaga gtatggagaa ttaaatttgg
      421 taattcctag agatagaaat ggtaagtttt ctcaacaaac tttacctgct tataaaagaa
      481 ccaatgactc tttagaaacc actattattc agctatttca aaaaggaatt actatgtctg
      541 aaatctctga attgattgaa aaaatgtacg ggcatcacta tacgccacaa accatttcaa
      601 atatgactaa aatagtttct gaagatatta ttgcctttaa agaaaggtcc ttagaatcca
      661 gatactctgt catttttatg gatgctactc acattccttt aaaaagacaa actgtttcaa
      721 aagaagcagt ttatattgta attggcattc gattagatgg aaccaaagag gttcttgggt
      781 ttagtcttgc tccaactgaa tctgcttatg tttggaaaga gattcttcaa gacctaaaag
      841 accgtggttt aaaagaggtt ttattagtcg taactgatgg tttaagtggc attaacgata
      901 gtattcatag tgtttatcca aatgctcaat ttcaacaatg ttgtgtccat atatctagaa
      961 atatcgctca taaggttcgt gttagtgatc gacaagaaat ctgtagcgat ttcaaattag
     1021 tttatcaagc aagttcaaaa gaagaagcaa ataatcaaat aaggtttatg atagataaat
     1081 ggaaaaagca gtatccacga gtggttaaat tgctcatgaa tcctgctata ttaacctttt
     1141 ataatttccc tccgtcaatc agaagaacta tttattcaac taatttgatt gaaggtttta
     1201 acaaacaatt aaagaaatat accaaaagaa aagagcaatt tcctaatgaa gaatctctgg
     1261 agagatttct agtatctcaa ttcaacaact ataatcaaaa gtttctatgc cgagttcata
     1321 aaggttttaa agaaatacat gatacattag aatcaatgtt ttaacttgat caacagaatg
     1381 ggcttttcca tttacacaaa attattgacg ctcccgacaa aattatgatc ttgcgtaaag
     1441 ttataaaata ttaataaa
//
LOCUS       DAKBVG010000315         1451 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.323, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000315 DAKBVG010000000
VERSION     DAKBVG010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.323"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(5..1144)
                     /locus_tag="OUD09_002682"
     CDS             complement(5..1144)
                     /locus_tag="OUD09_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HCT9693896.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT          376 a          314 c          264 g          497 t
ORIGIN      
        1 aatcttacgc taagtaggat aaaataattc cttgatattt ttcgatcata tccaaataat
       61 tatcgatttc aacatactca tctacttgat gaggcaaagt aacgacacca ggcccaaaga
      121 cgacaaagtc gaaggaatgg ttagctttcg taaattcggc tgcatccgtt gttgctgccg
      181 ctccaactaa aggtaatggc tggctgaatt gttgctgaat gcaatgaatc aaaggagaat
      241 ctggatcagc tttgacaggt attttgttgt aatcgatcat caattccaaa tgatagtctg
      301 tctcttgatt caattcattg acgattgatt gcagcaatgc aatgatttta tcatttgggt
      361 attctggaat cgatcgaatg ttcccttgga gctgcgcatg gcttgggatg ctgttgactt
      421 gattcccgcc actgatcaat gtaacattat ggattgtttt tcctaggaca ggattctcga
      481 ttgtttcagc gaggtgattc atttcagcat ttgctttcgt aatgaattca tttaggtgat
      541 tgattgcatt ataaccttgg tctggcatag agctatgcgc ttcttttccg tgcgaggtga
      601 ctgtatagtt gattgatccc atgtgcgtat acatcaaact gtaattggtt ggctctccga
      661 tgatcaaggc atctaagtca tctacatagc ctgctttcgt gagctgttcc ccacctagtt
      721 cacccacttc ttctccgaca gtggctaata atttgactgt tccgttaaat ggttttcctg
      781 actctttcag ttcgatcatg gcaatcacca ttgctgccaa gccactcttc atatctgtag
      841 caccacgtcc gtacaaacgg ttcccatgga tttctgctgc aaagggtgca taggtccaag
      901 acgattcatc accagccgcc acgacatcca tatgcccaga taggcctaac acttttcctg
      961 attggccctt ttggtaacga gcaatcaaat tatctctacc atctctgtaa gaaacaatct
     1021 ctcctgttat gtcatgtttt gccaaaagct tattcaaata ggctgctact tcaccttcgt
     1081 ttccattcac tgatttaatt cggatgatct cttgtaaaat cgcgattttt tcttcttttt
     1141 tcatctaatc ctctcccttg ccatttcttt ttattgtatt tgagtgtgct cgaattttca
     1201 aataggagca accaaagtaa aaatgaagaa agagagccac tttatgaaaa atatgctgct
     1261 acttccctcc tattttattt atacgagagg acttgctcat agattcctat tctactgttc
     1321 tatccttaaa agctcttata tgatgctagt tattgttagc tttccttttt caaggatgat
     1381 cttttcttgt accattcttt gtaaacatca tatacagaag ctagttgcaa aaaaactctc
     1441 tacctgctga t
//
LOCUS       DAKBVG010000316         1432 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.324, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000316 DAKBVG010000000
VERSION     DAKBVG010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.324"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            197..460
                     /locus_tag="OUD09_002683"
     CDS             197..460
                     /locus_tag="OUD09_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCT9693897.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            460..732
                     /locus_tag="OUD09_002684"
     CDS             460..732
                     /locus_tag="OUD09_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HCT9693898.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            809..1138
                     /locus_tag="OUD09_002685"
     CDS             809..1138
                     /locus_tag="OUD09_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCT9693899.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            1128..1415
                     /locus_tag="OUD09_002686"
     CDS             1128..1415
                     /locus_tag="OUD09_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCT9693900.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
BASE COUNT          535 a          175 c          283 g          439 t
ORIGIN      
        1 tatgcgtttt tttgttgatt taccaacatg tttagacatt ttcctctagg aaaaaattaa
       61 ctagcaccac gggtataaag gaaacggata acactttaca atgctgtttt ggtactccaa
      121 tttaaacaaa acaatcgaat ttacgtactt aattaagtac gttagaatgg agatataaat
      181 tagaggggga gtagatatgg caaaaacagt cttaaatcct agctcagcac ggaaaaattt
      241 ttataagttg ttgaaagatg taaatgagaa tcatacggaa attgaaatca ttagtgagcg
      301 cagtgaaaat aatgcggtgc ttattggttt ggatgattgg aaagcaatca aagaaacgct
      361 attacttgag caaataggca ctttgggtgt tgttcgtaag cgagaaaaag atgatagtgg
      421 atttactaat gttgatgaaa ttgattggga taacttataa tgaataacta ttctgtgatg
      481 attaaaaact cggctaaagc tgatttaaag aaaatcaaac agtctaattt gaaacctcaa
      541 tttgagaagg ttattcagac tttaaaggaa gatccctatc tccctaccca atcatttgag
      601 aagttaaaac ctacacatga aggtaggtat tctagaagat tgaatagaca acaccgggtt
      661 gtttataagg tagatgaaga tgaacacgtg gttgaaattt attcagcaag gacgcattac
      721 gagtcacagt aattacttgt aaatacatta tctcgtggta taatgtattt actaagtaat
      781 tacatactgt tagaaatgag ggatttaaat gattacaaaa acaagaaaac aaggcaattc
      841 aatcatgttg acagtaccta aagattttaa tgttcctaat ggagtcgaag ttgaagcaaa
      901 gttggtagag aatggtattc tttatgaatt tgttgaacca caaaaagaat tttatgattt
      961 tagcgaagat attttgtctg atataattgc agaaggctac gataaagatg agattcttgt
     1021 agaatttaag aatagaaaaa acaaaatgca ttcttccttt agagatattg ctgaagatac
     1081 acttactaat tctaaagtaa tgactaaaga ggagttggca acagaaattg gcttatgaaa
     1141 tcgttccttc aaaacatgtc attaagtatt taaaaaagct aaaagagaag ccattgaaag
     1201 aaaaattttt gaatttaatt tatgatgaaa ttgcgattga tccatataag ggtgaacaaa
     1261 aaactgggga tttatcaggt atttggtcta gtgggttcaa atatgcgggc acaacatata
     1321 gagtagctta cgagattata gaaaacaagg ttattccagt attgttatgt ggaacgcatg
     1381 aaaactttta tgaacagttg aaaaaattaa tctagtgatt tgttgtcgtt ct
//
LOCUS       DAKBVG010000317         1419 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.325, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000317 DAKBVG010000000
VERSION     DAKBVG010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.325"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            <1..>1419
                     /locus_tag="OUD09_002687"
     CDS             <1..>1419
                     /locus_tag="OUD09_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCT9693901.1"
                     /translation="SDADKYNPEGQKVTTELNKEPDASEGIKNKEDLPKDTKYTWKEK
                     VDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEGEKVEVGGTVDL
                     TDNVTNLPTLPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDN
                     RSDADKYTPMVEGEKVEVGGTVDLTDNVTNLPTLPEGTTVTDVTPDGTIDTNTPGNYE
                     GVIEVTYPDGTKDTVKVPVEVTDNRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDL
                     PKGTTYTWKEKVDVSTAGNKKGIVVVTYPDGSKEEVEVTISVEDKKAPNKPQVDPITE
                     GDQIVTGKTEPNAEVTVTLPNGSQYHGTADKNGNFTVKVPKLEAGTKVIVTATDESGN
                     TSEPTNVVVSSNEKDSEKAVSKDNKTDNQGSKQNKNRGKSSPQKQSSKAYPKTGEIDS
                     NIFTISGGLILLGTLGLLGYKNR"
BASE COUNT          566 a          222 c          314 g          317 t
ORIGIN      
        1 atctgacgct gacaaatata atcctgaagg tcaaaaagtg actacagaat tgaataagga
       61 accagacgca tctgagggaa ttaagaacaa agaagattta ccaaaagata ctaagtatac
      121 ttggaaagaa aaagtagatg ttagcgcagc tggaaataaa aaagggactg ttgtagtgac
      181 atatccagat ggatcatctg atgaagttga agtagatgtt acagtgacag acaatcgttc
      241 cgatgccgat aaatatgaac caacagtaga aggcgaaaaa gtagaagttg gaggcacagt
      301 agatctaaca gataatgtta ctaacttgcc aacattacca gaaggaacaa cagtaacaga
      361 tgtcactcct ggtggtacga ttgataccaa cacaccaggt aattacgaag gggttatcga
      421 agtaacgtat ccagatggta cgaaagatac agtgaaagtt ccagtagaag tgacagacaa
      481 tcgttctgat gccgataaat atacaccaat ggtagaaggt gaaaaagtag aagttggagg
      541 cacagtagat ctaacagata atgttactaa cttgccaaca ttaccagaag gaacaacagt
      601 aacagatgtc actcctgatg gtacgattga taccaacaca ccaggtaatt acgaaggggt
      661 tatcgaagta acgtatccag atggtacgaa agatacagtg aaagttccag tagaagtgac
      721 agacaatcgt tccgatgccg ataaatacaa tcctgaagga caaaaagtga ctacagactt
      781 aaataaagag ccagacgcat ctgagggaat taagaacaaa gaagatctac caaaaggaac
      841 tacttatact tggaaagaga aagtagatgt tagtacagct ggaaacaaaa aagggattgt
      901 agtagtaaca tatccagatg gatctaaaga agaagtagag gttactattt ctgtagaaga
      961 taaaaaagca ccgaataaac ctcaagttga tcctattaca gagggtgacc aaattgttac
     1021 tggtaaaact gaaccaaatg cagaggtgac agttacatta ccaaacggga gtcaatacca
     1081 tgggacagct gataaaaatg gtaattttac agttaaagtt cctaaattag aggcaggaac
     1141 aaaagtgata gtaactgcaa ctgatgaatc tggtaatact agtgaaccta ctaatgtagt
     1201 tgtttcttca aacgaaaaag acagtgaaaa agccgttagt aaagataata agactgacaa
     1261 tcaaggtagc aagcaaaaca aaaatagagg caaaagcagt cctcaaaagc agtcctcaaa
     1321 agcttatcct aagacgggag agattgatag taatatcttt actattagtg gtggtttaat
     1381 cctgttagga actttagggt tattagggta taaaaatcg
//
LOCUS       DAKBVG010000318         1409 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.326, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000318 DAKBVG010000000
VERSION     DAKBVG010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.326"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            171..1136
                     /locus_tag="OUD09_002688"
     CDS             171..1136
                     /locus_tag="OUD09_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HCT9693902.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
BASE COUNT          393 a          267 c          239 g          510 t
ORIGIN      
        1 aaaccatcta taatagaaat gtacaacaaa acagttgctg atccaactag taacttagat
       61 gaagacaatc agctgtctgc ttgctcgtaa tcatacattc ctattcattt tacaatgtat
      121 tcgttcatct tggtactaaa atctattaaa taaaaaggag aatattatat atgaagaact
      181 ttttgaaaat tattccgatc ccgatctgcg ggttgatcct tgggttagct tcccttggaa
      241 atctgtttaa agactatcac catgttgctt taggcaatct aactggcggt atctctatga
      301 ttcttatgat attcattttt gttaaattgt ttatcttatt cgaacacacg aaacaaacgt
      361 tgcatgatcc aatcatcgcc tccgtttcac caacttttac gatgtcactc atggttattt
      421 gtacttattt tgtttcatat gaaagtattg caccaattgt caaattcatt tggctgtgcg
      481 ctgttatctt ccaagtgatc cttgtctttt atttcaatta tcaccatgtt atcaaagctg
      541 atctttcaat tgaagcaatt tatccaagct ggtttatcat ttatgttgga tttggggtca
      601 tcactgtaac tgccgggaac ttcttcccgc ttattggaaa aattttcttt tggatttcat
      661 tgatctgcta tgcgttctta ttgccgatca tcatcaaacg tgtctttttc gtaagaaata
      721 tggctcatcc gacacgtccg ctgattacga tcgttgcagc acctggttct ctttgcctaa
      781 caggttatct taaaaacttt gctgaaccta atttttatct tgtcgtagca ttgttcgctt
      841 tatctcaagt attgtatttc gttgtgctgt ctatgcttcc acgtttgttc aagcttcgtt
      901 tttatccaag ctatgcagcc tttacattcc cgcttgttat ctctgcaaca gctatattta
      961 ctactgttca ttacttcaac agtttaggta tctatagtag cgtattggat ttcttaaaag
     1021 ttgcggaatg tctgtttgca agtgtagttg tcttctacgt tcttgctcat tacgtacgtt
     1081 tcttaattaa agaacatagc aaacatggaa aatttgtttc taaaaaaatc gcttaataat
     1141 cgattcaata ggttgtgaaa aagctgttct gcatcaagta ataagaacgg ccaaacaaaa
     1201 acagctgttc atgtttttga attggttggg cttattaccg cagatgcaag cttttgaaca
     1261 ccgtttacca agaggtcgta aaagaagtgg ccagcttcaa gaaataagaa ggaatttacg
     1321 aaaattgctt ctcaaatttt tgtgaaattt cggcttattt ccgaaaaagc tacttctgga
     1381 acaccgttta ccaagaggtc gtgaaaaag
//
LOCUS       DAKBVG010000319         1376 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.327, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000319 DAKBVG010000000
VERSION     DAKBVG010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.327"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            17..1312
                     /locus_tag="OUD09_002689"
     CDS             17..1312
                     /locus_tag="OUD09_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HCT9693903.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
BASE COUNT          427 a          274 c          261 g          414 t
ORIGIN      
        1 cataggagga ttcaagatgt ctcatactca gcttatcaaa gataccctaa atattctaga
       61 cctaaatatc tatttcgagg aaaattgttt aactaaagaa aaatacaaag ggcaaatttg
      121 cttgatttac aaaggaactc ttctgtacaa gccagaagag tgttcccatt gtctttgtgt
      181 cgtcccctct aggatcattc gttgggggac gacaaccgtt cggttgttac tgaatgatgt
      241 gtctgagtat cgcacttatc tggagctgaa gaaacaacga tttaaatgta agtcttgcca
      301 aagaacattc gtagcggata cctctgttgc tgaaaaacac tgtttcatta gtcaaaaagt
      361 tcgttggtct gtcattgctc gcttaaaaga gaatacttcc atgacggaaa tcgctcgtca
      421 gaaaaatatt tccacttctt cggtctatcg tgtcatgaag cgattctacc gcccattgaa
      481 tcctttcaaa cagactttgc caaaggtctt atgctttgat gaattcaaat cggttcgaag
      541 tgttgccagt gcaatgagtt ttatcatgat ggacggacaa agccatgcat tattggatat
      601 tgtcgaaaat cgacgcttgc cttatttaga acgctatttt tctcggtttt cattagcgac
      661 tcgtgaagcc gttcaattga ttgttattga tatgtatgcg ccttatgttt cgttggtgaa
      721 aaagcttttc ccgaatgctc agttgatcat cgatcgtttc cacatcgtcc aacatattgg
      781 cagaactttc ttgaatcatc gagtgaaaga aaccacggta cgacttaaag gaaattccca
      841 ctctcaacga gggttaggga aaaaattgaa gcgctattgg aaactcctac aaaaagaaga
      901 agcaaagtta aactatgaaa aacgggtttg gcgtgccagt tttaaagatt atttgactga
      961 aagtgagatt gccgatcgac tgttagctgc ttgtcccaac ttacgccaag gctatcagtt
     1021 ataccaagac attctttatg cggtaaagaa aagagatcag tcactatttg aggattgttt
     1081 aactagagac attagcggtc tgccagaaac ctatacaacc actttacgaa cgtttaaaaa
     1141 gtttctgcca cagatccaaa atgcgcttca ctacagctat tcaaacggac cattggaatg
     1201 cttaaacaac cacatcaaag tattaaaacg caatgcgtac ggctttcgca acttttacaa
     1261 tttcaaacta cgaattatga ttcgtcatgg caaaacattt ttaaccaaat aaacagaaca
     1321 gaaagaggtt ttcttctttc tgttctgtcc aaattattct aagatttctc tttacc
//
LOCUS       DAKBVG010000320         1355 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.328, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000320 DAKBVG010000000
VERSION     DAKBVG010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.328"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            79..1308
                     /gene="arcA"
                     /locus_tag="OUD09_002690"
     CDS             79..1308
                     /gene="arcA"
                     /locus_tag="OUD09_002690"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HCT9693904.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT          457 a          243 c          294 g          361 t
ORIGIN      
        1 aatgagacaa aattatgaaa gtgattacaa aaggaggata tcctatacat gtaatcaaaa
       61 agaaggaggc aacacaacat ggataaacct attcacgttt tctctgaaat agggaaatta
      121 aagacagttt tactgaaacg gcctggacaa gaagtggaaa atttgacacc agatattatg
      181 gatcgtctgt tgttcgatga tattccatat cttccgattg ctcaagaaga acatgacaac
      241 tttgcgaaga cattgcaaaa tgaaggcgtc gagactcttt acttggaaaa attagcggca
      301 gaagcgattg acgcaggcaa tgtcaaagaa caattcttga ataaaatgtt ggatgaatct
      361 catattgcat cgaatgctgt aagagagggt cttcatgagt tccttctatc aatggaaacg
      421 caagaaatgg tagataaaat catggctggg gttcgtacaa aagatatcga agtacgttct
      481 agcagtctat atgatctgtc agcagacgat gactatccat tttacatgga cccaatgcca
      541 aacttgtact ttacacgcga cccttccgca tcaatgggaa atgggatgac agtcaataag
      601 atgacttttg aagcacgtcg tcgtgaatcg atgtttacag aatatatttt gaaacatcat
      661 ccaagatttg caaataaagg aatcgaagta tggttggatc gtgaaaatcc agatcatatc
      721 gaaggcggag acgaattgat cttaagcgat aaagtagtag ctgttgggat ttctcaacga
      781 actaatgcga aagctttaga aaaattagca cgccacctat tcgcaaaaaa ttcaggattt
      841 gaaaaagtgt tggcaatcaa aattccgaac aaccgtgcaa tgatgcattt ggataccgta
      901 tttacaatgg tagatcacga taaattcact atccatccag caattcaaag taaagacggt
      961 aagatggatg tctttacaat cgaaccagat ggtgatgata tcaagattac tcattcagat
     1021 aacttacacg aaacgatgaa agctgctctt ggattagatg atcttgtact gatcccaact
     1081 ggtaatggag atgagatcgt tgcacctcga gaacaatgga atgatggatc aaacacattg
     1141 gcaatcgctc caggtgttgt agtcacttat aaccgaaatt atgtatcgaa cgaattgctt
     1201 cgtagctatg gcatcaaagt gttagaaatc aattcaagtg aattgtcacg aggtcgtggt
     1261 ggtccacgct gcatgagcca accacttgtc cgtgaagact tgaagtaatt tgtttcctga
     1321 cggcagtctt actgtcagct gccgtcggga tttcc
//
LOCUS       DAKBVG010000321         1344 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.329, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000321 DAKBVG010000000
VERSION     DAKBVG010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.329"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(156..1297)
                     /locus_tag="OUD09_002691"
     CDS             complement(join(156..1069,1069..1297))
                     /locus_tag="OUD09_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCT9693905.1"
                     /translation="MAKAKKKFDEDFKKMILDLNQSGQSVEELAAQYGIATQTIYRWK
                     KLHTKNVATGMTEAEILAMKKEMARMQEENTIPKKGFNHIRSKVKMSDVFEFIQSNVH
                     DHDVKQMCTVLEAPKSSYYDWKKKKPSKRQSENKRLNRLISGIYFENKGIYGAPKIHK
                     ILVGRGETLSLKKVQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSIN
                     QKWVTDITYIHTLKDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDNALLNQTP
                     SENLIIHSDLGSQYTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYP
                     SKAYENFEAAYLALFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
BASE COUNT          331 a          269 c          283 g          461 t
ORIGIN      
        1 gacacgattt gtgagaattt tttcgagatt ccagcgggtg agtatctcat cgtttcgacc
       61 agcggtcaaa ccaaaaccgt ttgaccgctg atcgaagcga tgaagttgaa accctgtgga
      121 aatctcgcca aaaaaatgta agtggtagct aggaactaag cttgcaatgc caactcctcc
      181 atctgatttg gtgttaaata gttgatacta ctatggattc gcttgcgatt gtaaaatcct
      241 tcaatgtatt gaaataacgc taaataagca gcttcaaagt tttcatacgc ttttgatgga
      301 tagacttctt cttttttgag tgaggcgtga aacgattcga taccggcatt atcgtaggga
      361 cagcccttac gactgaagga gtgccgaatg ttcaattcat tcagctttgc ctcataggct
      421 tcgctagtgt actgagaacc caaatctgag tggatgatca agttctcgga gggtgtttgg
      481 tttaagagcg cattgtcaag ggtttcgata accaattctt tcgtcatctg cttgccaaac
      541 ttccaaccga tgactttctt tgtgtggagg tcttgaatag tggagaggta ggtccagcca
      601 tcttttaggg tatgaatata ggtaatatct gtcacccatt tttgattgat cgacgtagtg
      661 gtaaaatctt ggtttaacag gtttttacgt ccttcacttt taactttcgc ttgtttgcca
      721 ggcttgtact tcttgagggt gactgaacgc aagcctaatt ttctcatcag tatttgtact
      781 ttctttaagg acaaggtctc gccacggcca acaagaatct tatgaatttt aggcgcacca
      841 tagataccct tgttttcaaa gtagattcct gaaatcaagc ggtttaagcg tttgttttct
      901 gattggcgct tggaaggttt ctttttcttc cagtcatagt agctggattt cggtgcttcc
      961 aaaacggtac acatttgttt tacgtcatga tcatgaacat ttgattgaat aaattcgaag
     1021 acatcactca tttttacttt tgagcgaata tggttaaagc cttttttagg atagtgtttt
     1081 cctcctgcat gcgggccatt tcctttttca tcgctaggat ttctgcctca gtcatgccgg
     1141 ttgctacatt ctttgtatga agcttcttcc aacgataaat cgtttgtgta gcgataccat
     1201 attgcgctgc cagttcttcg accgattggc cagactggtt taaatctaag atcatcttct
     1261 taaagtcttc atcaaatttt ttcttggctt ttgccatcgt ggacacgtcc ttttcttagt
     1321 agttagattc taccaaatcg tgtc
//
LOCUS       DAKBVG010000322         1337 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.330, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000322 DAKBVG010000000
VERSION     DAKBVG010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.330"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            124..1293
                     /locus_tag="OUD09_002692"
     CDS             124..1293
                     /locus_tag="OUD09_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HCT9693906.1"
                     /translation="MTKFTIEIMQTLLNKGDLDELFQDHLERAINSLLQAELTAFLDY
                     EKYDRNGFNSGNSRNGNYSRTFKTEYGELNLTIPRDRNGEFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMAEISKLIEKMYGHHYTPQTISNMSKLVAEDVLAFKERTLEANYSVI
                     FMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAPTESAYIWKEVLQDLRKRG
                     LEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIAHKVRVRGRKEICEDFKLV
                     YQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFYNFPHAIRRTIYSTNLIEG
                     FNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRIHKGFKEIQDTLESMI"
BASE COUNT          484 a          212 c          239 g          402 t
ORIGIN      
        1 cccatatttt aaaataaaag atagcgtaaa atattttgtg taaatagaaa tttagggtag
       61 aaaaaaaagt ccattctgta aaattaaagt taccacacag aaattttata ggaggacttt
      121 ctcatgacta aatttactat agaaattatg caaacactac tcaataaagg ggatttagat
      181 gaattatttc aggatcattt agagcgagcg attaattcac ttctacaagc agagctaact
      241 gcttttttag attacgaaaa atatgatcga aatggattca attcaggaaa ttcccgtaac
      301 gggaattatt cacgtacatt taaaacagaa tatggcgagt tgaatttaac tattcctaga
      361 gatcggaatg gcgaattttc tcaacaaaca ctaccagcat ataagcgaac caatgattca
      421 ttagaaacga ccattatcca attatttcaa aaaggcatta ctatggctga aatctccaaa
      481 ttaattgaaa aaatgtatgg tcaccactat acgccacaaa caatctccaa catgagtaaa
      541 ttggtggctg aagatgtttt agcttttaaa gaaagaacgt tagaagccaa ttattccgtt
      601 atatttatgg atgcgacgca tatccctgtg aagcgacaga ctgtttcgaa ggaagcagtg
      661 tatattacga ttggtattcg tttggatgga accaaagaag ttcttgggtt tactattgcc
      721 ccaactgagt ctgcttatat ttggaaagaa gttcttcaag atcttagaaa acgtgggtta
      781 gaagaagttt tattagtagt gacagacgga ttaagctgta ttgaagaaag tatccatagt
      841 gtgtatccga atgcccaatt tcaacaatgt tgtgtgcatg tatctagaaa tatcgctcat
      901 aaagttcgtg ttcgaggtcg aaaagaaatt tgtgaggatt tcaaattggt ttaccaagcg
      961 aattcaaaag aagaggcatt ggatcacatc gactttatga ctaggaaatg gaaaaagcag
     1021 tatccaagag tcgtcaattt actcttgaat cctgccctat taacctttta taatttccct
     1081 cacgccatca gacgaacaat ttattcgacg aacctgattg aaggctttaa taagcagcta
     1141 aaacgatata ctcgaagaaa agaacaattc cctaatgaag aatctctaga gagattcttc
     1201 atttctcaat ttaatcaata taaccaaaaa tttttaggta gaattcacaa gggatttaaa
     1261 gaaattcagg atacattaga gtcgatgatt taactgtaat taacagaatg gattttccat
     1321 ttacatataa ttcttga
//
LOCUS       DAKBVG010000323         1310 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.331, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000323 DAKBVG010000000
VERSION     DAKBVG010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.331"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            46..483
                     /locus_tag="OUD09_002693"
     CDS             46..483
                     /locus_tag="OUD09_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693907.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFENIEKNGAFYTIESEEKDNE"
     gene            476..1183
                     /locus_tag="OUD09_002694"
     CDS             476..1183
                     /locus_tag="OUD09_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693908.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNRLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELTVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
BASE COUNT          504 a          208 c          276 g          322 t
ORIGIN      
        1 cgaatattgg ttaaccaaat tagagaacta aaaaggagaa aaaatatggc tcaaaattac
       61 tatgacgaat ttgtcaaatt acctttggat aaaatggcac aaaaaatgga ggatatgacg
      121 ttcctatacc atgaaacacg tgtacccaaa aaacattaca aggaaaaatt gtcagtcgca
      181 gtggaagaaa tgattgaatc aggtgtcgaa atgaatctca ttgcgacgta ctatcgaaca
      241 ctggaagaac tgaaaaaaca aaatggaaaa tggtttttcc aagccttact atgcttggaa
      301 gtaggtgtca aaccaagcac tatcaaacct tccgaatatc aagccttaga actgacttat
      361 gcaaaattcg cacagattaa gaaagctaaa gcaatggcta gcgactggct agatactttt
      421 gaaaatatcg aaaaaaatgg tgcattttat acgattgaat cggaggagaa agataatgaa
      481 taaggtggtt tatcagctaa caacagaaga tgtttcgcaa tttttaacaa acaaagaaca
      541 cttgaatgaa gtaatcagac ggattcaaaa agactttagt tgtatggaag aaatgaagta
      601 ctttattcaa agagtggaag aacagttgta tatggaaagt gaaacgagtc aaacgaaggt
      661 tacagtcagc cataatcgtt tagacgtgta taaacgtcgt cctgacacat taaaaaaagg
      721 agcggagctt ctacgactag aaacagaaaa atatatcatg aatgtttacc tagaaaggag
      781 tgagctggtc gaaaactttg tcagctacac gatttgcagg aaacactatt actcagaatc
      841 agaatattat tcaatttttg agagcatgga attaatggaa gaagaaatgg aagagctaaa
      901 cagcaaagat gcgacggtag acgattatta ccttgtattt actagatttt tgacgtgtgg
      961 gacggaatct atcgtgttaa tgggaatgga ttatgaactc actgtacccg aagcattttt
     1021 gagggaatat gtagaagaag tagataaccc aggcgacgac tggtacacga ctttaactga
     1081 ttttttagac acctacactt acgacgatac agaagaaatc caaaatgaac ttgaccgtaa
     1141 acgaatgcca tatcaacgat ttgaaaggaa aaaaggtcaa taaccattta taccgaaaag
     1201 tgaggatgtg tgaaataaga acaaaaaacc ttgaaattaa gggaatggtt cttatttcgc
     1261 accaatcgat ccaaccgccc ccgccgctta ggtggtgcga aataagcggg
//
LOCUS       DAKBVG010000324         1307 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.332, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000324 DAKBVG010000000
VERSION     DAKBVG010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.332"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            3..977
                     /locus_tag="OUD09_002695"
     CDS             3..977
                     /locus_tag="OUD09_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCT9693909.1"
                     /translation="MRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAGQYLFD
                     KELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGAMTICL
                     SNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWETCLNEF
                     DKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFMEMQWIN
                     SNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVLDQADF
                     DLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            959..>1307
                     /locus_tag="OUD09_002696"
     CDS             959..>1307
                     /locus_tag="OUD09_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCT9693910.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLEL"
BASE COUNT          460 a          177 c          247 g          423 t
ORIGIN      
        1 aaatgagaca actacacaaa gaacagattg aaacagcaat ggcagcgtta aaggaaaaga
       61 caattgatca tgtatatttt acagcatgtg ggggttcaca agccgtttta atggctggtc
      121 aatatctatt tgataaagaa ttaactgtac cttgtcatgt ttatactgct aacgaattta
      181 actatgctat accaaaagga ttatctgaaa attctttagt tatcacttgt tcccattctg
      241 gcacaacacc agaaactgta gaagcaacaa aaaaagcaag agaatttgga gcaatgacta
      301 tttgtttatc taatgaagaa ggatctcctc tatgggaagc agctgaatat cctgttcact
      361 atgattgggg taaagatgtt gatgctagtg atttaaataa aggaattctt tatggtttat
      421 tatttaatat tttacttgtt ttagatggct ctaaaaaatg ggaaacttgc ttgaatgaat
      481 ttgataagtt aaatgatctt gttattgctg aaaagagcaa atttgaaata actgctaaag
      541 aatggggtaa aaaccaaaaa cgagataaaa ttatttacac aattggtagc ggtattaatt
      601 acggagaaat ttactccaca gcagcttgct ggttcatgga aatgcaatgg attaattcta
      661 atgctattca ttctggggaa tttttccatg gtccttttga aatcactgat tatgatgtac
      721 cattcatctt agtgaaaagt attggagata caagattttt agatcaacgg gtagaagact
      781 tcactaaaaa attcagtaat aaattaatgg ttttagacca agctgatttt gacttagaat
      841 ctatagatag tgtagcgaaa gaatatattg cagctgtact tagtggcgtt gttattcgcc
      901 aaatggtaga agcgattgct tttgaaagag gccattcctt agatgttaga cgctatatgt
      961 ggcaaatgaa atattaatta ataggaggtt gcaaaagatg gcaaatcaat tatttcacat
     1021 tgtgattaca acacattcta ccctttgtga gggttattta aaggcaacaa aattaatttt
     1081 aatgaatgac gccacaggta ttagtgctgt tccatttaaa gaaaatacta gtccagatga
     1141 ttttgaggag agtatgaaag aaataattag tcagcatcat ttgcaacctc taattatttt
     1201 aacggattta attggaggaa cacctaataa tgtttctatt aaacagttga acaacaatag
     1261 aattcaaatt attgctggga ttaatttacc tcttttactg gagttat
//
LOCUS       DAKBVG010000325         1299 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.333, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000325 DAKBVG010000000
VERSION     DAKBVG010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.333"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            19..>1299
                     /locus_tag="OUD09_002697"
     CDS             19..>1299
                     /locus_tag="OUD09_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HCT9693911.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          436 a          252 c          252 g          359 t
ORIGIN      
        1 gagaggacga tacgctatat gaatgattct atcaaaaaaa tgctgagaat aacagagaaa
       61 gatttgatga ttacagaggt ctcttacgag acccttcaga agaaaaagac gttggtcgtc
      121 gatgctgttc tctcgcctac tcctcgtgct tgtagaagtt gtggttctac tgtggtagat
      181 ggaaacggga aagcaattat agtgaaaaat ggaaaaaagg aaacgattgt ccgttttgaa
      241 caatacaatc atatgccttt ggttatgcgc ctaaaaaagc aacgctatac ctgtaaaaac
      301 tgtcgcaccc attggacggc tcaaagttat tttgtccaac ccagacattc aatcgcaaat
      361 catgttagat ataaaattgc ttctttactg actgaaaaag tatctttatc ttttattgcg
      421 aaaagctgtc aggtatcttt gaccaccgtt attcgtacat tgaaagagtt taaaagctat
      481 ttaccaaagc aatctaagaa gatcctcccc agagtattga tggttgacga atttcgttcg
      541 catgcttcca tagaagataa gatgagcttt atttgcgcgg atggcgaaac aggaaaatta
      601 atagatgttt tgcctacgcg taaattacct cgattaacaa gctatttctt aggttgtacc
      661 aatccagaag aagtagaatt cttggtgaca gacatgaacg ccgcctactt ccagctcacc
      721 aaacgtgttc tgccaaatgc gaaagtcgtg attgatcggt ttcatattgt caaacacatg
      781 aatcaagcgt tcaatgagtt gcgtatccgt gaaatgaatg aacttcgtaa agccggacag
      841 aaaagccagg cagaaaaact gaaaaagaac tggcgctttt tgctaaaaaa tcgtgcaaac
      901 atcaaccatt atgaatacaa aacatggaaa agtttccgag caccaaaata cccatttctt
      961 actgaagcaa tgatgattga tcgattgctt gagttttctc cgcccctaaa ggaggcgtat
     1021 cccttttttc atgaattagt tgaagccttt cgagacaaag accctgactt atttttctcc
     1081 ttattggcag aacttcccga aacgttagat gacagctttc gggaaaagct tcaaaacctt
     1141 ctgacctatg aagaaggcat caccaacgca atgatctatc cttattccaa tggaaaaata
     1201 gaagcgaaga atacccacat aaagacaatg aaacgagtat cctacggatt taaatcattt
     1261 gagaacatga gaattagaat ctttttgatc aatcaatta
//
LOCUS       DAKBVG010000326         1295 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.334, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000326 DAKBVG010000000
VERSION     DAKBVG010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.334"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            19..1200
                     /locus_tag="OUD09_002698"
     CDS             19..1200
                     /locus_tag="OUD09_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCT9693912.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
BASE COUNT          360 a          214 c          259 g          462 t
ORIGIN      
        1 tggaatagga gtgattattt gagttattta ttaggtatta ttggtttatt tgtcacatta
       61 gggcttgctt ttttgatcag cagtaacaaa aaagcaatca aattgaaacc actaattgtg
      121 atgttcgttt tacagcttgt tcttggtttc gtattattac gtacaacttt tggtacagcg
      181 gttgtttctg tattggcaaa aatgtttgat catcttcttg cttttgctgg gcaaggtgtt
      241 gactttgttt ttgccggtgt agcaaataaa ggaagtgcac ctttctttct ttccgtattg
      301 atgccaattg tatttatttc tgctattatc ggtattctac gctatatcaa gattcttcca
      361 ttatttatga aagctgttgg tctaggatta agtaaaatca acgggatggg caaattggaa
      421 tcttataatg gagtagcttc tgctatttta ggacaatcgg aagtttttat ttccataaaa
      481 aaggaattac ccttcctatc tgagaaacgt ttgttcacca tgagtgtatc ggctatgtct
      541 acagtttcta tgtcgatcgt cggatcttat atggcactga tcgattctaa atatgtgatc
      601 actgcccttg ttttgaactt atttggcggt tatattctgg cttcaattgt aaatccctat
      661 gtattagaag aaaaagaaga tgagctgatc attgaagaaa acaaagaaca gacatttttc
      721 caaatgttgg gagaatacat tcttgatggt ttccatgtgg cgatcacggt tgctgctatg
      781 cttatcggtt ttgttgcttt gattgccatg atcaatgcta tcttccacgg gatattcggc
      841 atcactttcc aagagatcct aggttacatc tttgcccctc ttgccttcat cagcggaatc
      901 ccatggaaag aagcagtgga tgccggtagt atcatggcga ctaaactggt tacaaatgaa
      961 tttgttgcta tgacagaatt agcaaatggt aatttccatt ttactgagcg tacaacagca
     1021 attatatctg tcttcctcgt ttcatttgcc aacttctctt ctatcgggat catttctggt
     1081 gcgatgaagg gattaaatga agaaaaagga aatcttgtcg caaaacacgg attgaaaatt
     1141 ttgctaacag cgactttagt cagcttttta agtgctattg tgactggctt gctggtataa
     1201 tatacaaaaa agcaaagtta cttttaaaag ctttggaaat attttccaaa gctttttcac
     1261 ttgttcaatc atcaaaggag aaatgacaat gaatt
//
LOCUS       DAKBVG010000327         1246 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.337, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000327 DAKBVG010000000
VERSION     DAKBVG010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.337"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            200..742
                     /locus_tag="OUD09_002699"
     CDS             200..742
                     /locus_tag="OUD09_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693913.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..>1246
                     /locus_tag="OUD09_002700"
     CDS             898..>1246
                     /locus_tag="OUD09_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HCT9693914.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          514 a          141 c          238 g          353 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccag
//
LOCUS       DAKBVG010000328         1239 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.338, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000328 DAKBVG010000000
VERSION     DAKBVG010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1239
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.338"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            <1..700
                     /locus_tag="OUD09_002701"
     CDS             <1..700
                     /locus_tag="OUD09_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCT9693915.1"
                     /translation="KKNTKVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTS
                     VKYYDEIANSLTEALYSTIRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENM
                     FLRVGVQAKAVLDPHFQAQVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIA
                     ITNYIHSPIGKSADLVLQTAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVEL
                     REKVLRSIIDKSIE"
BASE COUNT          438 a          186 c          204 g          411 t
ORIGIN      
        1 taagaaaaac acaaaagttg gcgatgctac tattattcgc ttttgtcaaa aaattggttt
       61 ttctggtttt tctgatttaa aaattgaaat tgcaaaggaa gactttagtc aaaaaaaaga
      121 acatccgact agtgtaaagt actatgatga aattgctaat agtttaacag aagctctcta
      181 ttccactatt aggcttttag acgaagaaaa attgaatgaa gccattaaaa tgatcactca
      241 atcaaaaaac atatatatat ttggagtggg atctagtgga aatactagtc ttgatttaga
      301 aaatatgttt cttcgcgtag gagttcaagc aaaagctgta ctagaccctc attttcaagc
      361 ccaagtcgct tctttattaa cagttaatga tttagtaatc attttttctt tatctgggaa
      421 aacgaaagat acttatgatt cattgaaaat agctaaaaaa aatggcgcaa aaattattgc
      481 tataactaac tatattcatt cacctatagg aaagtctgca gacttagttt tacaaacagc
      541 cattgaagaa tttcttaatg gtggatcttt agctggaaaa atatcacaac tttatatttg
      601 tgatctatta gttcatggtt atgaacaaaa caataatatt gattcagttg aattaagaga
      661 aaaagttttg cgctctatta ttgataaaag tattgagtga actaccccca cttagctaaa
      721 cctaacggtt tttaacgatt gaagtaggca attcttggga tgagagcata cttgatagct
      781 tattttttac taatagcggt ggctcttatt gaccaaatat ccctgtagtt cctacggttc
      841 ttgattttat ctaaagatat ttttatcgtg gaagctattg atcaacttga aagaaaaacg
      901 tgtagctcat tatcactagt ttatcgagta tggatgaagc aatggatcat cctctcttaa
      961 ataaatttat taaggatctg atccttcaaa ttttttccat gatcgatgag caggaaccta
     1021 ctgaatctaa acgacatcgg gcacaaggaa ttattaagat tgctaagcga atgttttcta
     1081 taaagggtgt tctaagctat acagtgttaa ttcaaaagat tcccaatcat cttgtttata
     1141 agaacaacgt tgaagattaa aaaagtgttt caatgctaaa attgcaaaat acttcaatgt
     1201 tatgagacaa actgtctata agaatcaatt tccacccat
//
LOCUS       DAKBVG010000329         1215 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.339, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000329 DAKBVG010000000
VERSION     DAKBVG010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.339"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            414..947
                     /locus_tag="OUD09_002702"
     CDS             414..947
                     /locus_tag="OUD09_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HCT9693916.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          389 a          226 c          225 g          375 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggacaccgt ttacc
//
LOCUS       DAKBVG010000330         1178 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.340, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000330 DAKBVG010000000
VERSION     DAKBVG010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1178
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.340"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            33..1139
                     /locus_tag="OUD09_002703"
     CDS             33..1139
                     /locus_tag="OUD09_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HCT9693917.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRTRYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
BASE COUNT          368 a          252 c          254 g          304 t
ORIGIN      
        1 atacaaaatt aatttagaaa gtaggttgcc aaatgaataa cgtaaaaatc aatgaactac
       61 gtcaatggat ggcacaacac caagtcgaat tggcttatat cagcgaccct ggccacatcg
      121 cttacttttc cggatacgaa agtgatcctc atgagcgtgt actagctctt tttatccctc
      181 ttgaacaaga tcctttcctt ttcactcctg ctttggaagt tgaagatgct caaaacagta
      241 gttgggcgta tgatgtccgc ggctatttag acagcgaaaa cccttgggag atcatcggaa
      301 aagaacttcg tactcgctat ggtcagcctg gcaatgtagg gattgaaaag aacgccttga
      361 ctgtcgagcg cttcgatgct atctctgata tcttgacggg aacggattct tacactgatc
      421 taacttccat catccaacgt ctacaactga tcaaaactct agaggaaaaa gacaaactga
      481 tcgaagctgg taaatgggcc gatgttgcat ttgagattgg ctttaaagca gtgaaagaag
      541 gcgtgactga acaagcgatc attgcagaaa ttgaatacca attgaaaaaa caaggtgttt
      601 cacaaatgtc tttcgacaca ttagtccttg caggagcaaa tgctgcaagc cctcatggta
      661 caccaggaaa tacgaagatt tcaccgaatg aacttgtgtt attcgatctt ggcgttgttt
      721 ggaatggcta ttgcagtgat gctactcgta caattgctta tcaaaaacca accgacttcc
      781 aagaaaaaat ctacaacatc actcttgaag cacagcttgc cgcacaagaa gccgtccgtc
      841 caggtgtgac agcaggtgaa cttgatcaaa tcgcacgaaa cgtgatcaac agctatggct
      901 acggcgaata tttcaatcac cgtttaggtc acgggatcgg aacaaccgtc cacgaattcc
      961 cttcccttgt agaaggaaat gacttagtaa tcgaagaagg gatgtgcttc tctcttgaac
     1021 caggaatcta tattcctgaa aaagtagggg tacgtatcga agactgtgta tatgtgacaa
     1081 gcgatggctg tgtgccattc acgactacac caaaagaatt acttgtttta gaaaaataag
     1141 agtaaaaaag gtgagagcta gtccgctccc accttttt
//
LOCUS       DAKBVG010000331         1165 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.341, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000331 DAKBVG010000000
VERSION     DAKBVG010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.341"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(82..171)
                     /locus_tag="OUD09_002704"
     tRNA            complement(82..171)
                     /locus_tag="OUD09_002704"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(133..135),aa:Ser,seq:tga)
     gene            complement(195..268)
                     /locus_tag="OUD09_002705"
     tRNA            complement(195..268)
                     /locus_tag="OUD09_002705"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(232..234),aa:Ile,seq:cat)
     gene            complement(282..355)
                     /locus_tag="OUD09_002706"
     tRNA            complement(282..355)
                     /locus_tag="OUD09_002706"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(319..321),aa:Met,seq:cat)
     gene            complement(383..455)
                     /locus_tag="OUD09_002707"
     tRNA            complement(383..455)
                     /locus_tag="OUD09_002707"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(420..422),aa:Ala,seq:tgc)
     gene            complement(475..548)
                     /locus_tag="OUD09_002708"
     tRNA            complement(475..548)
                     /locus_tag="OUD09_002708"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(512..514),aa:Pro,seq:tgg)
     gene            complement(558..631)
                     /locus_tag="OUD09_002709"
     tRNA            complement(558..631)
                     /locus_tag="OUD09_002709"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(595..597),aa:Arg,seq:acg)
     gene            complement(642..727)
                     /locus_tag="OUD09_002710"
     tRNA            complement(642..727)
                     /locus_tag="OUD09_002710"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(691..693),aa:Leu,seq:taa)
     gene            complement(744..815)
                     /locus_tag="OUD09_002711"
     tRNA            complement(744..815)
                     /locus_tag="OUD09_002711"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(781..783),aa:Gly,seq:gcc)
     gene            complement(827..899)
                     /locus_tag="OUD09_002712"
     tRNA            complement(827..899)
                     /locus_tag="OUD09_002712"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(864..866),aa:Thr,seq:tgt)
     gene            complement(917..998)
                     /locus_tag="OUD09_002713"
     tRNA            complement(917..998)
                     /locus_tag="OUD09_002713"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(962..964),aa:Leu,seq:tag)
     gene            complement(1006..1078)
                     /locus_tag="OUD09_002714"
     tRNA            complement(1006..1078)
                     /locus_tag="OUD09_002714"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1043..1045),aa:Lys,seq:ttt)
     gene            complement(1082..1154)
                     /locus_tag="OUD09_002715"
     tRNA            complement(1082..1154)
                     /locus_tag="OUD09_002715"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1119..1121),aa:Val,seq:tac)
BASE COUNT          303 a          331 c          278 g          253 t
ORIGIN      
        1 ggagcaggat ttgaacctac gaccttcggg ttatgagccc gacgagctac cagactgctc
       61 catcccgcga taatatgaaa aaggaggata agggattcga acccttgcac ggttttaccc
      121 gcctgacggt tttcaagacc gttcccttca gccggacttg ggtaatcctc catatgaaac
      181 ttacaacgcc acaatggacc ttgtaggact cgaacctacg accggacggt tatgagccgt
      241 ctgctctaac caactgagct aaaggtccag gctcttaaaa atcgcggcgg aggggatcga
      301 acccccgacc tcccgggtat gaaccggacg ctctagccag ctgagctaca ccgcgaaaaa
      361 gatattcatt atttaaatga aatggagcct agcgggatcg aaccgctgac ctcctgcgtg
      421 caaagcaggc gctctcccag ctgagctaag gccccgtgtt taaatttata attatcggga
      481 agacaggatt cgaacctgcg accccttggt cccaaaccaa gtgctctacc aagctgagct
      541 acttcccgtt taattaacgc gcccaagagg agtcgaaccc ctaacctttt gatccgtagt
      601 caaacactct atccagttga gctatgggcg catatttgaa atgccgagga ccggaatcga
      661 accggtacgg tgatcactca ccgcaggatt ttaagtcctg tgcgtctgcc agttccgcca
      721 ccccggcgcg attgctttgg caaagcggaa aacggggttc gaacccgcga cccccacctt
      781 ggcaaggtgg tgctctacca ctgagctatt tccgcgtgtt taaagatgcc ggctaaagga
      841 cttgaaccct cgaccctctg attacaaatc agatgctcta ccaactgagc taagccggca
      901 tgtaaaactt aaattatgcg ggtgaaggga cttgaacccc cacgccgtaa ggcgctagat
      961 cctaaatcta gtgcgtctgc caattccgcc acacccgcaa aaatatgagc cgtacagggc
     1021 tcgaacctgt gaccctctga ttaaaagtca gatgctctac caactgagct aacggctcat
     1081 atggaggtta acgggatcga accgctgacc ctctgcttgt aaggcagatg ctctcccagc
     1141 tgagctaaac ctccatggat aactt
//
LOCUS       DAKBVG010000332         1161 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.342, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000332 DAKBVG010000000
VERSION     DAKBVG010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.342"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            29..>1161
                     /locus_tag="OUD09_002716"
     CDS             29..>1161
                     /locus_tag="OUD09_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCT9693918.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQY"
BASE COUNT          419 a          178 c          242 g          322 t
ORIGIN      
        1 tttgggtttt tgaatggagg aaaatcacat gaaaattatt aatattggag ttttagctca
       61 tgttgatgca ggaaaaacta ccttaacaga aagcttatta tataacagtg gagcgattac
      121 agaattagga agcgtggaca aaggtacaac gaggacggat aatacgcttt tagaacgtca
      181 gagaggaatt acaattcaga caggaataac ctcttttcag tgggaaaata cgaaggtgaa
      241 catcatagac acgccaggac atatggattt cttagcagaa gtatatcgtt cattatcagt
      301 tttagatggg gcaattctac tgatttctgc aaaagatggc gtacaagcac aaactcgtat
      361 attatttcat gcacttagga aaatggggat tcccacaatc ttttttatca ataagattga
      421 ccaaaatgga attgatttat caacggttta tcaggatatt aaagagaaac tttctgccga
      481 aattgtaatc aaacagaagg tagaactgta tcctaatatg tgtgtgacga actttaccga
      541 atctgaacaa tgggatacgg taatagaggg aaacgatgac cttttagaga aatatatgtc
      601 cggtaaatca ttagaagcat tggaactcga acaagaggaa agcataagat ttcagaattg
      661 ctccttgtac cctgtttatc atggaagcgc aaaaagcaac atagggattg agcagcttat
      721 agaagtgata acgaataaat tttattcatc aacatacaga aagaagtctg aactttgcgg
      781 aaatgtcttc aaaattgaat attcggaaga aagacaacgt cttgcatatg tacgccttta
      841 tggcggaatc ctgcatttgc gggattcggt tagaatatcg gaaaaggaaa aaataaaaat
      901 tacagaaatg tatacttcaa taaatggtga attatgtaaa attgataagg cttattccgg
      961 ggaaattgtt attttgcaaa atgagttttt gaagctaaat agtgttcttg gagatacaaa
     1021 gctattgcca cagagagaga gaattgaaaa tccgctccct ctgctgcaaa caactgttga
     1081 accgagcaaa cctcaacaaa gggaaatgtt acttgatgca cttttagaaa tctccgacag
     1141 tgacccgctt ctacaatatt a
//
LOCUS       DAKBVG010000333         1154 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.343, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000333 DAKBVG010000000
VERSION     DAKBVG010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1154
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.343"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(257..1129)
                     /locus_tag="OUD09_002717"
     CDS             complement(257..1129)
                     /locus_tag="OUD09_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCT9693919.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT          314 a          276 c          224 g          340 t
ORIGIN      
        1 atttgagtgt aacataaatg gcttttttat ttgtctcgct taattataca aacggcgtgt
       61 aaaaaactat tgcacaatac aagtggccta cggctatcga aatttccata actttcgagc
      121 aagaatttat ctcattcaag gcttgatctt ccaagtaaaa caaaaaccag ttaagcactc
      181 tgcctaactg gttatgacta aattttcagt tgtatctttc tctaccaaca ctaaatgtcg
      241 aagagccttt tttcgtttat gaatgtaaga ccgcttctcg agccaatgcc aaacaaccga
      301 gcgttccagc gttgtcttct aatgctgggg tcacgatata tttgcttaac tcaggtgttt
      361 cgacataccc attgaccaat tttgcaaatg cgtcgtggac gttttgcagc atatggcgtt
      421 gtttcatcac accaccgcca aaaatgatca catctggaga aaacatcaag gttgtattgt
      481 aggcacattg agcaatatag gaggcttcga tttcccaata aggatcatct tctgcaacct
      541 cttgtccttt tttccctaag cgtttttcga tggcgggtcc agcggccatc ccttctaaac
      601 agttgccatg aaatggacag ctgccagcaa agtcatcttt tggatgaggg acgacgagca
      661 tatgccccat ttctggatga ctaaaccctt cgataaagcg cccttcttgt aaggcaccgc
      721 cgccaacacc cgtaccgatc gtatagtaaa cgacactgct caatcctttg ccactgccga
      781 agacatactc accataggca gccgcattga catctgttgt ccaagaaatc gggatcggaa
      841 attctttttt catggtaccg atgaaatcaa aattttgcca tgccaatttc ggggtcgagg
      901 taatgtaccc ataagtggcg gaatcgcgat ggatatcgat aggcccaaat gagccaatcc
      961 caatccctgc taaatctgct tggtattgtt tgaagaagtc aatgactaaa ggcattgttt
     1021 cctcaggtgt cgttgtcgga aagctcactc tttccttgat cgtcatttct tcatcgccaa
     1081 tggcacacac aaattttgtg ccgcctgctt caattgcacc gtatagcata gacatttctc
     1141 cctttatttt agaa
//
LOCUS       DAKBVG010000334         1139 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.344, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000334 DAKBVG010000000
VERSION     DAKBVG010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1139
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.344"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            1..797
                     /locus_tag="OUD09_002718"
                     /pseudo
     CDS             1..797
                     /locus_tag="OUD09_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS256-like element ISEfm2 family transposase"
     gene            complement(914..1057)
                     /locus_tag="OUD09_002719"
                     /pseudo
     CDS             complement(914..1057)
                     /locus_tag="OUD09_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608918.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
BASE COUNT          399 a          194 c          193 g          353 t
ORIGIN      
        1 actacacgcc ccaaaccatg tccaacatga ctaaagtgct gactgaagaa gtaaatgcat
       61 ttaaagttag aactctaaat gataagtatg tcgctatttt catggatgct acttatattc
      121 ctttaaaacg gcaaaccgtc tccaaagaag caatctatat tgccattggt atacgagaag
      181 acggcactaa agaagtacta agttatgcga ttgctccgac tgaatcgacc tacgtttgga
      241 atgagttgtt acaggacatt tactccagag gggttcatga tgtcttactg tttattaccg
      301 atggcttaaa aggcatgaaa gatacgattc accagattta tcctaaagct aaataccaac
      361 attgctgtgt tcacgtttcc cgtaatattg ctcataaagt acgtgtcaaa gatcgaaaag
      421 aaatctgtga tgattttaag gctgtttatc aagctagctc aaaggaagag gcaaatacct
      481 ttttagggag tatgattgag aagtagcaaa aaacttatcc taaagtgacg cagtcactga
      541 taaaaaatca agatttattg actttttatg agtttccgcc tggtatccgc cgaagcattt
      601 actcaactaa tttaatcgaa tctttcaaga aacaaatcaa gaaatacagc cgcagaaaag
      661 agcagtttca aaatgaagaa tcactagagc gtttcctagt atccatcttt gatacataca
      721 atcaaaaatt cttaaataga agccataaag gcttccaaca agtgacggat acattagctt
      781 caatgtttac tgagtaaccc attattttgc agaaggacga gttatttaca caaaattatt
      841 gacgctccca ataaaaaaac aaacaaagta aaataatcta ctttgtttgt ttttatttta
      901 attaatattg aatttataaa attgtcttat cgcttggttc cacaaccaag tagcgataag
      961 gctcatcccc ttcagaatga gtggtaacat aaggattttt acttaaaaca gaatgaattt
     1021 gttttctttc aaaagcgggc attggttagt caaagactag ttcatttata tttatatctt
     1081 aatcttttat tttaataaat tgtgatataa tgaagtataa ctttttaggg agtgtacta
//
LOCUS       DAKBVG010000335         1133 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.345, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000335 DAKBVG010000000
VERSION     DAKBVG010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.345"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(176..>1133)
                     /locus_tag="OUD09_002720"
     CDS             complement(176..>1133)
                     /locus_tag="OUD09_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCT9693920.1"
                     /translation="DRYGDRVKNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNY
                     HKVLATAKAVEIYRSGNYGGRIGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDP
                     MVKGYYTEEFLELCQKQSIYFDPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNE
                     ETPFHPERYYEVFDLPGKKMNNSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGR
                     EHEERYMDEKGIVQDDYRIEFIGQHLRYLIKATEEGANCEGYMLWAFTDCVSPMNAFK
                     NRYGLVRIELNDERSRTLKKSASWYQTLIDKRELDLPDEIYK"
BASE COUNT          332 a          242 c          174 g          385 t
ORIGIN      
        1 cgaataaacg gtattcaaaa gcttgcatct gcggtaataa gtccaaccaa gttaaaaaca
       61 taagatgttg tttttacttt gctattctta ttacttgatg caggacagct ttttcacaac
      121 ctcttttatt tcaaataacc agtaaataca atgaacatct acaaaaaact tctatttatt
      181 tatagatttc gtcaggcaaa tccaattctc tcttatcaat caaagtctga taccacgaag
      241 cagatttttt caatgttctg cttcgttcat cgttcaattc gattcgaact aatccatatc
      301 gattcttaaa tgcattcatt ggtgacacac agtcagtgaa tgcccataac atatagcctt
      361 cgcaatttgc gccttcttct gttgctttga tcaaataacg caaatgctgt ccgataaatt
      421 cgatacggta atcatcttgc acgattcctt tctcatccat ataccgttct tcatgttctc
      481 ttcccatacc gttttctgtt atcagccaag ggatattatg ataattttct tttagataca
      541 tggctatatc atacatgatt ttcggataga tttcccatcc cctggaatta ttcattttct
      601 tcccaggtaa atcaaagact tcataatatc gttctggatg gaaaggtgtc tcttcattcc
      661 attgataacg aggagcgcga acgcgtttag gataatattg attgattcca aggaaatcta
      721 ctgtatgatg ctgaatcgtt tctaaatcct ctgttgcagg atcgaaataa atcgattgtt
      781 tttgacacaa ctccaggaac tcttctgtgt agtacccttt taccatcgga tcaaaaaata
      841 ctcgattata aaacaaatca tacatataag cagctttttg gtcttctgga gaattagatc
      901 gagcatagac catttcagga tttaaaatac agccaatcct tccaccataa tttccgctgc
      961 ggtaaatctc tactgcttta gccgttgcta aaactttatg ataattccac atcatccact
     1021 tttttgtgtt ttgttcatgc ggccagcgaa cagcatccaa atagcaacgc gtttggggaa
     1081 cgattggttc gttgaatgta aaccagtttt ttaccctatc accgtaacga tca
//
LOCUS       DAKBVG010000336         1133 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.346, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000336 DAKBVG010000000
VERSION     DAKBVG010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.346"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            <1..537
                     /locus_tag="OUD09_002721"
     CDS             <1..537
                     /locus_tag="OUD09_002721"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693921.1"
                     /translation="VMAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNG
                     QPMNDKRNGHSWFRQQERIENKVHSLREEIKQQEKQVEKLERQEEIKEMGYNKYGGLD
                     MTIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQA
                     IIDSGRVTQWKKIRPFIF"
     gene            564..>1133
                     /locus_tag="OUD09_002722"
     CDS             564..>1133
                     /locus_tag="OUD09_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693922.1"
                     /translation="MNENGEFEESKKYQATSEEEKAAVRELLEEAVPTDFLKEKENDH
                     FYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYYKFYFEEVKNGEVV
                     DQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKEQMQSKETKTKQKG
                     YQKRTAEEIKQEVKSLSQQALDAVKKHTHS"
BASE COUNT          479 a          179 c          252 g          223 t
ORIGIN      
        1 gtcatggcag aagaaaaaga accactagac aatagtcgtt tgggaaaatc caaaagaaaa
       61 ttagtacgat tgcaaaatga attaaacgaa caaattgaaa agatgtttga acaccagaga
      121 aagaccaacg gtcaaccaat gaacgataag agaaatgggc attcttggtt taggcaacaa
      181 gagcgaatcg aaaataaagt gcatagtctg agagaagaga tcaagcaaca agaaaaacaa
      241 gtagaaaaac ttgaaagaca agaagagata aaggaaatgg gctataacaa gtacggcgga
      301 ctggatatga cgatcgagaa tattccaaga atcaaagaag aaatcgaacg ctttgaaaaa
      361 ggtgaatcga ctttttcagc cgctacgatc cgaaaatacc aacggaaatt ggaaacatta
      421 gaacagctga aagaaagatc ggaaaaagga aaagaaaacc ttcttcctga agtacaagca
      481 atcattgaca gcggacgagt gacgcaatgg aaaaaaatcc gaccatttat tttctgaaag
      541 gctatcgaaa agtcgcttta gaactgaatg aaaacgggga atttgaggaa tctaaaaaat
      601 accaagccac gtcggaagag gaaaaagcag ctgttcgtga attacttgaa gaggctgtac
      661 caactgattt tttgaaggag aaagaaaacg atcattttta tcgagtagaa ttcaacgaaa
      721 atgatccaga aaaaggattg aaaagttatg gtggggagat cgtaacattc gaattgatcg
      781 atcaactcaa aacgttggat aaccaatacg ccgcagaagc aggttattat aaattctatt
      841 ttgaagaagt gaagaacgga gaagtagtcg atcagatccg aatggatcta ggggatggcg
      901 tcaacagtaa tcaacccatc tatttagagt tagcaaaatt gtgcgaacaa gtacctgaga
      961 aggaacaaaa acaatcggtc aaaaatgaac ctgtttcgaa agaacaaatg cagtcgaaag
     1021 aaacaaaaac aaaacaaaag ggctatcaaa aacgaacggc agaagagatt aaacaagaag
     1081 ttaaatccct ttcgcaacag gcactggatg cggtgaaaaa gcatactcat tct
//
LOCUS       DAKBVG010000337         1118 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.347, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000337 DAKBVG010000000
VERSION     DAKBVG010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.347"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            12..1052
                     /locus_tag="OUD09_002723"
     CDS             12..1052
                     /locus_tag="OUD09_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HCT9693923.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
BASE COUNT          456 a          167 c          187 g          308 t
ORIGIN      
        1 gaggatctac aatgactgat tttaaatttt ttaaggcgga tagagtttac aacgaattat
       61 tttatcaatt tccaaaagtc tttattgttt ctgacgaata caaaaaaatg aaagattcaa
      121 ctaagattgc ctatatgctt ttaaaagcaa gattagagat cgcaatcagc aaacggcaaa
      181 tcgatgaaga aggtaatgtt tattttactt atacgacaaa tgaactatgt agagtattaa
      241 actgccaaaa acaaaaagcg atagcaatca aaaaagagtt ggaatccttt ggtttattat
      301 tacaaaagca gatgggattt aacaaacagt tagggaaaaa taatcctaat agactatatc
      361 tagcagaatt aaaagtctca gaaaatgata tctacttact cgaaaaattt gatagagaga
      421 atagggaaaa cgttgataaa tcagagggta tgaaaatcat acccaccctc gacgaaaaat
      481 cagacgctga atcccttggg gctcaagagg gtatgaaaat cataccgtgc caaaacgttg
      541 ataaatcaga gggtatgaaa atcataccag aacttaataa taatatatta gacactaata
      601 gacacaatat agacactgaa aaagaccgcc tacaagatca attgttgtta gacaattttg
      661 agacaattat gacaaacgac agcattgcta cgtttgtccc tgaacgagta ttaaatttga
      721 taaaaacatt ttcttcaagt tacagtgaag ctcaaaaaac cgtccagact attcataatg
      781 caaagaaaaa agctgaaata gaaagtgata tttcgatagt ttttgaagaa ctcgatagtt
      841 attatgtcaa tgcagaacaa gaattataca cgacactgtt aaaagcctat caaaaattaa
      901 aaaccgaaaa agtcgaaaat atccagaacc tgatttttgt ctatgtaaaa aattggttta
      961 tcgaaaaacc aatagctgct aaagtatcaa gtgaaaaacg tttgaattat gcaagctccc
     1021 caagcactat tacgaaagac tggttagagt gaaactttac tgacaaagtg attgttgctt
     1081 catggagtaa cattaaaatt aaaaattttt tattattc
//
LOCUS       DAKBVG010000338         1092 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.348, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000338 DAKBVG010000000
VERSION     DAKBVG010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1092
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.348"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            <1..279
                     /locus_tag="OUD09_002724"
     CDS             <1..279
                     /locus_tag="OUD09_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCT9693924.1"
                     /translation="KRNPFVSEYVKRLAKGICQLCQEKGPFIKDGVPYLHCHHIEYLS
                     QGGKDVIENCIALCPNCHARIHELELPDDKEKLLKIVEKRENSFSKIP"
     gene            622..878
                     /locus_tag="OUD09_002725"
                     /pseudo
     CDS             622..878
                     /locus_tag="OUD09_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320124.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
BASE COUNT          379 a          164 c          198 g          351 t
ORIGIN      
        1 aaacgtaatc ccttcgtatc tgaatatgtt aaacgacttg caaaaggtat ttgtcaactt
       61 tgccaagaaa aagggccatt tataaaagac ggtgtacctt atttacattg tcatcatata
      121 gaatatttgt cgcaaggtgg aaaagatgtg attgaaaact gtatcgcgtt atgtcccaat
      181 tgtcatgcta gaattcatga acttgagtta cctgacgata aagaaaaatt gctgaagatc
      241 gttgaaaaaa gagaaaatag cttttctaaa ataccgtaat ttatttttat aggacaaata
      301 agctaatgtg agctatgcct tctatataag actaagttct attataagta acgaagtaat
      361 tctcgttcct tcctgtagta atatttttta aataacctat aggcttcttc aaaagtacat
      421 cctccagtaa attctaagta ctggaggatt ctttttctag tgttgcttta ctcatgaaaa
      481 agtagttggc tacttcatca aaatttctga acttttttaa tgtataataa agagctcgat
      541 catttactac aaggatgcta gctcctacat tttcacagtt ttttagcaag taaatttttc
      601 taaaatcaca tcaggaaatg catgagattc tttaagaaag gcaattatag ggctatgatt
      661 acggttcgtg taagtgacgc caaaaaagaa tggctttctt atatggctga attttatggg
      721 attactttat ctgaacttct aaaaaactat tcgatggaac aactagaaga tgaatacaat
      781 cgtcaaacgg ctgaaattgc ccataaacat tggataagaa gtgggaaaac aacagtattc
      841 atggaagaat ccttaatgaa tttggtgggc tggaatgacc tgccgtttaa aattcacgcc
      901 cgaaattcag aagcagctct gattggcaaa tacgtgtatt ggtagagata gaagataatc
      961 aattcgttgt taccgctatt caagtagggc accgaagaaa tgtctatgat taagagactg
     1021 tttattttta caaaaaaata ataactggaa tctagttaaa actcttgaag tgagagattt
     1081 gactagattt tt
//
LOCUS       DAKBVG010000339         1091 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.349, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000339 DAKBVG010000000
VERSION     DAKBVG010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.349"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            <1..344
                     /locus_tag="OUD09_002726"
     CDS             <1..344
                     /locus_tag="OUD09_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693925.1"
                     /translation="FIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVT
                     ITLSSGSAGYFMNDGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYA
                     PPIRNFFNDYY"
     gene            531..>1091
                     /locus_tag="OUD09_002727"
     CDS             531..>1091
                     /locus_tag="OUD09_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCT9693926.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
BASE COUNT          357 a          129 c          218 g          387 t
ORIGIN      
        1 aatttatagg aagttttaga gggaatgtga caactcaacc tagacgaagt tggagtataa
       61 aacttttaaa taatagtgga gagttgaaag catttcatta tgaattctta ataatagaaa
      121 gtgaagatgt aacaatcact ttaagtagtg gctctgctgg atattttatg aatgatggtg
      181 gaagcaagcc acagctcaca gtccgtgatc gtattgcagg ttttatgcat ttttctcact
      241 atgacttaga agaagctcgt aaaatacttg cagaatatgg gatcacatta atcaattggg
      301 aatatgcgcc accgataaga aattttttca atgattatta ttagtcttat aaaaaacaga
      361 atgtaaaatt tatgtgcgcg agtatttagt tttccatttt ttgtttgata gatatatagc
      421 attgttgaag catgggatag tggtgccaat tttatcaatg aggttggtca ggcagttaac
      481 ataattggtg atgctataaa tccatttaat tggaattgga ggaattaggt gtggaaacag
      541 tgagattatg gatattcatg aaaaattttc gttcggcttt gagatatgtt attctggaat
      601 caaaatcagg ttattatctg attgacacag atcactatac tttttgggga ggctaccttt
      661 ttccgatttt taattggata atcccccata aggttatgaa aattaacttg cctgcagaag
      721 aaataaatag attaatactc aacaaagatt tacaaaggaa aagagagaaa gaacattcta
      781 atgttgcttg gataggtcca gcatttgcag tagtgacttc ttcaattgtc ggtcctatgc
      841 tagagagtat ttatagtgat ttttctattg gaatacgtta tttatttgtt actttaagta
      901 tagctgttct tttgtgcata aagggttatg ttagcaagaa gaataaagat attttaacac
      961 taatcggtgt aaacaattta tctagtacta aaattatact gtttcctgaa tctataaaag
     1021 aaggatcaag gttgttgata atttttataa tgcttatact gttttctgtt ttatttgggg
     1081 tgatgtttat a
//
LOCUS       DAKBVG010000340         1077 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.350, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000340 DAKBVG010000000
VERSION     DAKBVG010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.350"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            192..446
                     /locus_tag="OUD09_002728"
     CDS             192..446
                     /locus_tag="OUD09_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCT9693927.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            447..764
                     /locus_tag="OUD09_002729"
     CDS             447..764
                     /locus_tag="OUD09_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCT9693928.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          377 a          168 c          221 g          311 t
ORIGIN      
        1 ggatgttaac ggaagtgtat ggtgagtagt tcactcgtat tttgatatcg tttgtatatg
       61 ttttcagcgt tctaagtggc ttattttgtc caataataat atggagtgga atcccttaag
      121 aaacaccgtt ggcaattgtt gttataacat gttataattt attcatcaaa aactaaaagg
      181 agtgtgataa catgggaaaa gaactaaagg ttagaaagat cggcaatagc gtgggagtga
      241 tccttccaag ctctcttgga ttaaaaagcg gagacattat ccaagccaaa caagaaggca
      301 acttattcat attagatact actcaaatag caaaagaaca cgatcgcaaa ttgattgaag
      361 aaagtttcca agactttgag aaaggtttaa cagtttcaga aattgaaatg gtaaaagctt
      421 tcggaaaata cggctggagt gaatagatga aagagtacac gctagtttat tctaaaagtt
      481 ttcagaccag ccttgaaacg ataatagaag aatgggaaaa tgagcttgct ctttctccag
      541 ccagcataaa aagatttgta ggtgcgatcc agaaggcttt agaactaacc aaaaattttc
      601 caactatgca tgaggaagtt tccgaaatct atggaatgga tatcccaaca tatcggatcg
      661 tgatcggcaa aagctatgcc ttattttatc ggatcgacca tgaaaaaaaa gagatacttg
      721 taggacgtat tttccaatct aaacaaatga aactaggctt ttaacaataa ttaggaaaaa
      781 gtcgctcatc ctctacaggt gggtgattaa tgccgctcga tatggttgtt aaccgtcggt
      841 tactcgaaga ttgatatata agacttagta cctttttatt ttgctccatg ttatggccga
      901 aggtctaaag atacaatcat tagtctagag aaaaattaag aaccgtagga actacgggga
      961 tcgcttggtg aataagagac actgctatta gtgaaaaaat aagatatcaa gtacgctcta
     1021 ttcccaaaaa tttcgtgact ttactcatga gaggttcaag tagtatattt ttaatca
//
LOCUS       DAKBVG010000341         1073 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.351, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000341 DAKBVG010000000
VERSION     DAKBVG010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.351"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            <1..>1073
                     /locus_tag="OUD09_002730"
     rRNA            <1..>1073
                     /locus_tag="OUD09_002730"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          329 a          214 c          308 g          222 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact aggatgttgg cttagaagca
      961 gccaccattt aaagagtgcg taatagctca ctagtcgagt gaccctgcgc cgaaaatgta
     1021 ccggggctaa acatattacc gaagctgtgg agtacacctt taggtgtatt ggt
//
LOCUS       DAKBVG010000342         1066 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.352, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000342 DAKBVG010000000
VERSION     DAKBVG010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.352"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(199..747)
                     /gene="aac(6')"
                     /locus_tag="OUD09_002731"
     CDS             complement(199..747)
                     /gene="aac(6')"
                     /locus_tag="OUD09_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HCT9693929.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          326 a          214 c          150 g          376 t
ORIGIN      
        1 aaaaatgtag aaagccattt tcacaaaatt tttcttacta ctcgaaacta agcgactttt
       61 tcacaacctc tggtacacgg tgttccaaaa gtagcttctt cggaaataag ttggaatttc
      121 acaaaaattc aagaagtaat tttcgtaaat tccttcttat ttcttgaagc tgattacttc
      181 tgtcacaacc tctttttatt attgagaatt tggtcgagga ataatcgttt ttgccatcca
      241 aatatccggt ttgtcccagc catttgcatt tggtaagaca cctacgattt tataacctaa
      301 tttttcatag aattcatacg gatgttcacg aaggttctgg atggaagcca ctttatcaaa
      361 tgtatgctca tacaggtccg tttgacttaa cgttgttcca tggtctaaat catccgtacc
      421 taaataaatc gtgattcctc ctctggaagc tacttctttt tctaagtaat tgactaatcg
      481 agtacctatt tggttctttc gtcgggagct ttctacaact aatggatgca attcccaacc
      541 tgtgataccg tattgaggga ttgcaccaat aaatcctact aactcatctt ggtctaccgc
      601 tgctaccgcg attcgttctg gattcatcat ttcttctact tcttctgccg agctgtctcc
      661 atattcttcc ggccaagtca gtctcagtaa atcagaaagc tgatctttca atactggatt
      721 attacggtca aattcactga ttatcatacg tcttccccct tgttcttgct tattctaaca
      781 gtccttttga aaaaacgaaa tgttctcgct caaaaatctt tagctaccaa tcagtatctc
      841 tattctttca ttatttatct tacttataaa ataaaaaaat tatacagaaa cactttattc
      901 aaaataggaa agatcaggaa agccatcaat tggaggaaaa aactctgttt gttcatcatg
      961 atatagatta tcttttgctt taaaaaaaac caattatgaa ttgacaaata aaacctgttt
     1021 tcatataata atcttaattt ttagaatttt tagataatta caaaaa
//
LOCUS       DAKBVG010000343         1059 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.353, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000343 DAKBVG010000000
VERSION     DAKBVG010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.353"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(37..993)
                     /locus_tag="OUD09_002732"
     CDS             complement(37..993)
                     /locus_tag="OUD09_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693930.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          344 a          176 c          160 g          379 t
ORIGIN      
        1 ttagaaaaag cagtcggact ttctaaaaga agttcactat tcgatcctgt cgatgatttc
       61 ttcaggaaaa tcgtctattt gcttgtcaat tttgctgtct ttgtatttct cttcttggat
      121 agtcaacaaa atctttgtaa gattccccca gtctactgct cttggcggat cgagccaaac
      181 cactacttga gatgcacttt cttcatatag attattccta ttcgtgatta taacatccgg
      241 gaactcaggc tttcctgcct ctatcacttc cgtgttcaga ttaatgtaca agcttaaatt
      301 tttcttgata aattgattat aggcagcacc ataagaaaaa tcgatgatga tctttactgg
      361 ttcagaaatc agttgaatgg gtatgtgatt gatcaataat aataaataac tgaaaaaaag
      421 aaaaaattta tcttgaagca actctttgcg ttttgacgtt agatcattta tatactctat
      481 caaatagcaa taaaatgctg gatagttttc tctaaaaaaa gaaacatcaa gtggaaattc
      541 atcagcttta ttaatagtgt aatgcaaaag tttaaaatgt attttggtta actctcttcg
      601 gatcaattgg ctcgtctctt ctcgcaacag attaaactga ttttcaaaac tttcaatgaa
      661 tcgttcattc aaccattcga ttgtttcatc ttcatttttt ctcaagtatt tttgatccaa
      721 tttatcatga agataaagcc aagagagtgt cttattgata aaaataacgg atttttctcc
      781 taatactcct tttaagtggt aaaatcctat atccgaaaaa aatagcgact gattgctagc
      841 aatatctaaa gaatcagctt gttcagccaa caggttgatt gctaaaaagt ggaataattt
      901 ctttttttca taagcagaca gaggataata ctgttttaaa tcttttaaca ttttttctgt
      961 ttttataatt ataggtagcg tatacaattc cacaaaactt atctctgttg agttaaaaag
     1021 attatataaa aaaagccgaa tgtttttagg attccctgt
//
LOCUS       DAKBVG010000344         1048 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.354, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000344 DAKBVG010000000
VERSION     DAKBVG010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.354"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            <1..560
                     /locus_tag="OUD09_002733"
     CDS             <1..560
                     /locus_tag="OUD09_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCT9693931.1"
                     /translation="IGITFSSFLALGVILIGVANSSTDLFHILFGNILAVQDIDKWIT
                     IGVSVLVLVTVVLFFKELLITSFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVG
                     TILIVAMLITPAATAYLYANSLKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLT
                     AAFLFVISFLISPKQKFLRKKERKL"
     gene            560..>1048
                     /locus_tag="OUD09_002734"
     CDS             560..>1048
                     /locus_tag="OUD09_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HCT9693932.1"
                     /translation="MRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVVT
                     NSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETGG
                     NAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKNI
                     EKQ"
BASE COUNT          360 a          132 c          205 g          351 t
ORIGIN      
        1 caattggtat tactttcagt tcttttttag cattaggagt tatccttata ggagttgcaa
       61 atagctcaac ggatttattt catatcttat ttggaaatat tttagctgta caagatattg
      121 ataagtggat tacaattggc gtttctgttt tagtgttggt tacagttgtt ctatttttta
      181 aagaattatt aatcacttct tttgatccat taatggcaaa agcaatgggg atgaatgtca
      241 gtttttatca ttatctatta atgattctat taactttagt agcagtaaca gctatgcaaa
      301 gtgtgggaac tatattaatt gttgcaatgt taattacacc agctgctaca gcatatttat
      361 atgcaaacag tttaaaaact atgatattta tttcggcgac agtcggagcg tgctcatcat
      421 tgctaggttt gttcattggt tatagtttta acatagcagc aggatcaagt attgttttaa
      481 cagcggcatt cttatttgta attagttttc taatctcacc aaaacaaaaa tttttaagaa
      541 agaaggagag aaagttatga tgagaaaatg gaaagcagta ctgggaagtc tggggatttt
      601 gattgctctt tttatattcg gggcatgttc aacaaatagt aaagacaaag atacagtggc
      661 ttcgaacgaa aaattaaagg tagtagttac taattcgatt ttagcagata ttactgaaaa
      721 tatagcaaaa gataaaattg atttacacag tatcgtacct attgggaaag atccccacga
      781 atatgaacct ttgcctgaag atgttcaaaa aacttcaaaa gcagatttga ttttttataa
      841 cggtgttaac ttggaaactg gaggaaatgc ttggtttaca aaattagtaa aaaatgcgaa
      901 caaagaggaa aacaaagact attttgcagc aagtgatggc atagatgtta tttacttaga
      961 gggtcagagt gagaaaggga aggaagatcc ccatgcttgg ttaaatttag aaaacggtat
     1021 tatttacgct aaaaatattg aaaaacag
//
LOCUS       DAKBVG010000345         1033 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.355, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000345 DAKBVG010000000
VERSION     DAKBVG010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.355"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            91..642
                     /locus_tag="OUD09_002735"
     CDS             91..642
                     /locus_tag="OUD09_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF334 domain-containing protein"
                     /protein_id="HCT9693933.1"
                     /translation="MKRLEASTSNFNNKLDVFEEKTKHARLNFETTAKHYIKRLDEDN
                     LKLDFQQAIQDELSDTKDEIREVTKQAREETKEYKEILESKIKDHNKVVDKSNTALKV
                     MTKGVTNIFFVLIIFVLVMLVTGPIGHFFGIEHLYSFINGFIDDHESAWRYLMLILYA
                     VPYVFFAIILWLIALFFRVFNDL"
BASE COUNT          393 a          144 c          196 g          300 t
ORIGIN      
        1 tagataccgt aagggatttt attctttatt taaaactttg caacagaacc gaagagcgag
       61 caagacagga acgagagaac aaacatactc atgaaaagac tagaggcttc gacctcgaac
      121 tttaataata agttagatgt ttttgaagaa aagactaagc atgcaagatt aaactttgaa
      181 acaactgcaa agcactatat taaacgactt gatgaagata atttgaaatt agattttcaa
      241 caagctattc aagatgaatt aagcgatact aaagacgaaa tacgtgaagt gacgaaacaa
      301 gcgagagaag aaacaaaaga gtataaagaa atcttagaaa gtaaaataaa agaccataac
      361 aaagtggttg ataaatcgaa tacagcgcta aaagtgatga caaaaggcgt tacaaatata
      421 ttttttgtgc taattatttt tgtacttgta atgcttgtga caggtccgat aggtcatttc
      481 tttggtattg aacatttata tagctttatt aatggtttta ttgatgacca cgaaagtgca
      541 tggcgatatt tgatgttgat actttatgca gtaccctatg tattcttcgc tatcattctg
      601 tggctcatag cattgttttt tagagtattt aatgaccttt aggtagattt atatataaaa
      661 agggctaaat acgcaaaata cccccttaaa atgcaaaata aatgcaaata agggggtaac
      721 tccttatgaa aaaacaacta atagtattat aacacgtttt tatgccgaga aaacttattg
      781 gttggaatgg gctatgtgtt agctaacttg ttagcgagtt ggttggactt gaattgggat
      841 taatcccaag aaagtaccaa ctcaacaaca cataaagccc tgtaggttcc gaccaataag
      901 gaaattggaa taaagcaacg aaaggagttg agtaaaatga gtgagaaaac gaagaaaatc
      961 attcgagtat cattagcttt ttcacaagta gtagtgcaat cattgatagt ttattatact
     1021 taccagcaat ata
//
LOCUS       DAKBVG010000346         1031 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.356, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000346 DAKBVG010000000
VERSION     DAKBVG010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.356"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            89..1024
                     /locus_tag="OUD09_002736"
     CDS             89..1024
                     /locus_tag="OUD09_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCT9693934.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT          356 a          166 c          232 g          277 t
ORIGIN      
        1 gttataaaaa tgaacattgc atagtagaga ttctttgatt atactaattc aaggttatat
       61 gaaaaaagag aaatgagatg gatgatttat gaaactttgg atgaaaatcc ttttaggcgt
      121 cgctttagct ctgattgttg cgttagtgtt tgcagggaat tatttttata attatgctgt
      181 tgtgccttct gagaaagact ttttagacga agatacacct ggtacaacac agacatccaa
      241 agaaacggat gcacataaat ggtttagttc aaaagaaaac cgaagcgatt ggaccatcac
      301 ttcaaaagac ggtctgaaat tatcagcgat ttatcttcct gctgaaacga aaagtgaaaa
      361 aacagctctt gtggctcatg gctatatggg aaacgctgaa acgatgacca attacgcaaa
      421 gatgtttcat gatatgggtt acaatgtcct tgttcctgat gcaagaggac atggaaaaag
      481 cgaaggggat tacattggat ttggatggcc agagcgtaaa gactatgtac agtggataaa
      541 caaagtgcta gaaaacaatg gaaaatcgca agaaatcgtt ctatacggtg tcagcatggg
      601 agcagcaaca gttatgatga ctagcggaga aaaactacca gataatgtga aagcaatcat
      661 tgaagattgc ggttattctt ccgttcatga tgagcttgct tatcagctag acgatatgtt
      721 tagtcttcca gcatttccgc ttatgcaagt aacgagtcta gtgacaaaag tccgtgctgg
      781 ctactttttc ggtgaagcaa atgcagtgga acaattgaag aaaaaccagc gcccaatgct
      841 ctttatacat ggagacgaag atacatttgt accatttgaa atgttagata aggtctatcg
      901 tgcaacgaag ggaccaaaag aaaagtatgt tgtaaaagga gccgagcatg cggaagcata
      961 caagacagat cctgaaaaat atcagcaagt agtccaacaa tttttaagcc aatatgttca
     1021 ttgaacgagt t
//
LOCUS       DAKBVG010000347         1026 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.357, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000347 DAKBVG010000000
VERSION     DAKBVG010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.357"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            58..414
                     /locus_tag="OUD09_002737"
     CDS             58..414
                     /locus_tag="OUD09_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCT9693935.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
     gene            404..670
                     /locus_tag="OUD09_002738"
     CDS             404..670
                     /locus_tag="OUD09_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCT9693936.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            829..>1026
                     /locus_tag="OUD09_002739"
     CDS             829..>1026
                     /locus_tag="OUD09_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HCT9693937.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVI"
BASE COUNT          423 a          132 c          200 g          271 t
ORIGIN      
        1 attcgaattt ttatgttata tactataata taaaatcaat atatgatata attgggtatg
       61 gaaaaaatat cttttgaaac atttaaaaga cctaatggtc atgatgaatt tttagaatgg
      121 ttggaaacat taccaaaaaa agatagtgcg aagcttttac gtacaataga agaaacagaa
      181 aagaatggga tgctagtagc tcaacggttg aaatgggtga aaaaattaga cactaatttg
      241 tacgaattaa gaagtaaggt aggatcaaac atccaaagag ctatctattt ccatgtggtc
      301 aatggaaggt atgtcattac ccatggattt actaaaaaaa cggagaaaac accaataagt
      361 gaacttaaac atgcaaaaga acttagagag gagtggttca caaatgatga ttaaaaaatt
      421 gattgaagaa aagcaaaaag acaaagattt tgcagaagca tatgaccgtg aaggaagaaa
      481 actcgaaact gcggttgctc tttatcatgc acgggaggaa gcaggcctaa ctcaagcgga
      541 gcttgcacaa aaagcacata caactcaatc tactattgct cgaattgaga gaggggataa
      601 cgtatctttc gataaacttg atgatatcgc tagggcatta ggcaaaaagc tggtagtaga
      661 atttgcttaa aagcgagttg aaaatatacc tgtaataggc tcctaactaa tcaattagtt
      721 tagtgggcct ttttgtacta aatagataaa agttgtccta tagtgtaaaa gtcaataaat
      781 tttatacaaa aattacttta aagagacata aagagaaagg tacaataaat gaaaaccata
      841 aatctaataa aagtatttat agcatcacca ggtgatatag cagcaaagag gaatgaagta
      901 gaagaaacca tatatagatg gaatatagaa aatacagata atacaaaagt aattttgatg
      961 ccaattcgat gggaaacgaa tgcgattcct acttatataa ataatagctc tgggcaaagt
     1021 gttatt
//
LOCUS       DAKBVG010000348         1018 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.358, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000348 DAKBVG010000000
VERSION     DAKBVG010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.358"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            188..>1018
                     /locus_tag="OUD09_002740"
     CDS             188..>1018
                     /locus_tag="OUD09_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693938.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     V"
BASE COUNT          377 a          122 c          180 g          339 t
ORIGIN      
        1 ttaaaagagg aagtccaata tatctatggg aaacttctta aatattatgt tggtacatga
       61 aaatatacat ccctgctaga ctatttacaa cattttactt tgtacgtatt cttgttttaa
      121 aaggaatggg ataaaatata ggtacaataa ctatttgatg ctaaatataa ttaatggagg
      181 agagatagtg aacatactta atgaaaaaag atatgtatgg tttgctggta aaaaatatat
      241 tactactttc aaaagattat ccgagttaat gcagcagact gttaaaagtg ctatgcctga
      301 aggatacaag tttaattttg ttcaatcttc agtatctact gaaagtatct atttttcaat
      361 tctttctgag agtaggcttt ttcattttag aataagttct catgtaaaga aaactgatgt
      421 tgatatatat acgttccgta caaataatta taaaaattta aatgatttga gtagaagtat
      481 cagtgaatat ctcaagggga atagctatct caagattcga ttttcacata tattaatttt
      541 actaccatat ttgaatgaga atttcaccct aattcaagta gataagtctt tcattgcctc
      601 aggtaatcaa ggaaatatga atattacaag taacaaaacg tcacgtgtgc tattggcgtt
      661 gatgggttac ggaattcttt atagagatga ccaaaatggc cagattctaa taaatcgggc
      721 tgggcgttca ttattaatga aatatagaaa atttataccg gaagattttc aaaacttaca
      781 gttagaaaac atgcgtttag aagatttatt tagattagca gaagatgata agtatagatt
      841 tgaagttttg aaaattcaaa agaatgaact attgaataag ttatatcctg agaatgcgat
      901 aaaagcacat tttggtgatg ctaatcatat gtatagttgg ttcattccta aaagatttag
      961 agggaaaatc aatgctggag atcaagtgaa agtactcaat cgaaatagta cagatgtc
//
LOCUS       DAKBVG010000349         1015 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.359, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000349 DAKBVG010000000
VERSION     DAKBVG010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.359"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            40..591
                     /locus_tag="OUD09_002741"
                     /pseudo
     CDS             40..591
                     /locus_tag="OUD09_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358527.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT          372 a          170 c          164 g          309 t
ORIGIN      
        1 tcatccatcg atcaaaatga acaacgtcaa attgaagaaa tgaaaaagtt tggcgctgaa
       61 aaaattttta tcgaaaaaca atctggagcg acgattagtc atcggccgat tttccaggaa
      121 gctttagatt ttgtcagaga gcaagatatt tttattgtag aagccattga tcgacttgga
      181 cgcaattatg acgagattat cgattcagtg aattatctaa agaagaagaa tgtgcgactt
      241 attatcacca gtttaccgat catggctgaa gcaattggaa atcctctcct agataaattc
      301 atcaaagact taatcattta aatctcagcc atgattgctg aacaagaacg tactgaatcg
      361 aaacgacgcc aggcacaagg aattaaaatc gctaaagcaa acggcgtcta taaaggtcgt
      421 cctaagttat acagtgcaga caccaaagat cctcaacgtc gccttgtcta taggagtatc
      481 gttcaggatc ttgagaatgg ggttgctatc tcaaagattg caacagacta caatgtgact
      541 aggcaaacaa tttaccgaat aaaaaaagaa atcgaccaac taattgtatg atttcttttt
      601 tgaatcacgt aattattaat tagctttgtc atcaatacag acaatcattt tagattcatc
      661 tagctaagtt gattacattt atgttaaaaa gaatttagtt aacataaata taattgatgc
      721 ataaatcgtc cttatttaaa ggcatttaac attgacaact tagtataatg tatcttatgt
      781 aaactaataa gatttgaatt ttattttata cacactcgat aatagatcaa ttttatggtc
      841 ccccgcattt acgattgcac aagagaaaca taaagtcgac ctattagcat ttcttttcaa
      901 ttcaaacatt aaaaaagttt atacctctgc tactaaaaaa tgaaattatc tatttaaaat
      961 ttaaaacggt aaatctgatt catccaaatt ggaaaatgga tcagcctgta ttgga
//
LOCUS       DAKBVG010000350         1001 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.360, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000350 DAKBVG010000000
VERSION     DAKBVG010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.360"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(<1..>1001)
                     /locus_tag="OUD09_002742"
     rRNA            complement(<1..>1001)
                     /locus_tag="OUD09_002742"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          210 a          311 c          227 g          253 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatgcaccgc g
//
LOCUS       DAKBVG010000351          998 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.361, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000351 DAKBVG010000000
VERSION     DAKBVG010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.361"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            11..931
                     /locus_tag="OUD09_002743"
     CDS             11..931
                     /locus_tag="OUD09_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCT9693939.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
BASE COUNT          324 a          165 c          197 g          312 t
ORIGIN      
        1 gagtaaaatt atgactgcgt atcataaaaa tgaaagatta aagaaaatag tggttatttt
       61 gataaccgga ttgaccgctg cggtcgcttt aaattatttt ttgattccag ccaatgtctt
      121 ttctgcaggg atgaatggga tcgctcaaat catttcctcg cttttatatg aatggtttca
      181 tatcaagata gacacaggaa catttatttt tcttttgaat atccctgtgt ttgtattagg
      241 gtttctcaaa gtaggaaaac gagcaacgat ccttagtttt gtgaatgttg tatgtgtatc
      301 ggtgatgacg atgctgcttc cagtcggaca gatcactgat aatattttga tgaatgccct
      361 agtaggcggt gttttattag gtcttggcgt aggattatct ttaaaaatgg gctttacaac
      421 tggtgggatg gatatcatct ctcttgtact ttcacaaaca actggaaaaa cagtcggaaa
      481 ctacatgctg attctaaatg gtatcattgt agtcatcgca ggcttcttct ttaactggga
      541 aagtgccttg tatacgatta tttccattta tgcaatgact caagtaattg atgccatcca
      601 cacgagtcat caaaaagtaa cagcaatgat cgttaccgtc aagccagaag aggtaacaga
      661 agaactagca aaacggatgg tacgtggaat gacattactg ccgtctgtcg gtggctattc
      721 aaaaaaacaa gggaacatga tcatgatggt catcacccgc tacgaaatgt atgatcttga
      781 tcagatcgtt catgaaatcg acgacgatgc atttatcaat attttaccaa ctcattcagt
      841 atttggccgg tttgcaaatg aaaacgaaca aaaaatgtat cgaacaactg gcgtattgcc
      901 agaaataaaa aatcgttcac aaaaaaaatg aggaatcagg tttattttag aataaattta
      961 atctattaca tctaataaga acatttttat aaatttaa
//
LOCUS       DAKBVG010000352          997 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.362, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000352 DAKBVG010000000
VERSION     DAKBVG010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..997
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.362"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            <1..929
                     /locus_tag="OUD09_002744"
     CDS             <1..929
                     /locus_tag="OUD09_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693940.1"
                     /translation="FDITQTQLKPEDYPKIFPNRVFDFQLDKKGQSELQAGIQAVANN
                     IGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNTRNTPTQHLATSIHELAHA
                     RMHKFSELDTATKELQAEMTSYIVCKHFGMDTLEKAIPYIAAWTKNGQTLDDKEAAER
                     GKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQEHKNTVETAEQKNIVPVGS
                     LWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGKSYGKEIQTALPSEEFALG
                     NSFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
BASE COUNT          379 a          160 c          215 g          243 t
ORIGIN      
        1 tgtttgatat cacacaaaca cagttaaaac ctgaagacta tccgaaaatc tttccgaacc
       61 gtgtctttga ttttcaattg gataaaaaag gacagtcaga acttcaagct gggattcaag
      121 cggtagccaa caatattgga atacaaattc gagatatgac agaaagtgaa gtctatcaaa
      181 gagaattagg acaagcacgt ggggcgtatg ttcgtaacga atttactggc gaagaagaaa
      241 tcgtcatgaa tactcgaaat actcctacgc agcatttagc tacaagtatt catgaattag
      301 cccatgcaag aatgcacaaa ttttcagaat tagataccgc aactaaagaa ttgcaggcag
      361 aaatgacttc ttatattgtg tgcaaacatt ttggaatgga cacgttggag aaagctattc
      421 cttatattgc agcatggacg aagaatggtc agacgttgga tgataaggaa gctgcggaac
      481 gtgggaaaat catgaatgat gtcagccgtg tcgcaaatga attcattcaa accatttcta
      541 atgaaatcaa tcaatacaga gaaagagaac cagagatcca accagcagaa gtgaagcagg
      601 agcataaaaa tactgtagaa acggcagaac aaaagaatat tgttcctgtt ggttcacttt
      661 ggatcgacaa gaaagacggg aaagtaatgg aagaagatac gggtcgaacg ttacatttga
      721 aggatgcggc gtttattgaa gatccacagc ataatcaagt aattttaaaa ggaaaaagtt
      781 atggaaaaga gatccaaacg gcgttacctt cagaagaatt tgcgctagga aattcattcc
      841 gattttcact tgagacagtg tcagaagtgg caaaacgaaa aaatgaaatc tattatgaac
      901 aacaattgtc acaaaaagaa cgtgtataaa acaagtaaaa aagactatct gaaaaacgga
      961 tagtcttttt tcttttggga ggttttaaaa tgaccga
//
LOCUS       DAKBVG010000353          975 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.363, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000353 DAKBVG010000000
VERSION     DAKBVG010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..975
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.363"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(53..787)
                     /locus_tag="OUD09_002745"
     CDS             complement(53..787)
                     /locus_tag="OUD09_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HCT9693941.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDKLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
BASE COUNT          337 a          202 c          181 g          255 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agcttatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaactcca actcttttta
      961 aaagttctcc acaaa
//
LOCUS       DAKBVG010000354          956 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.364, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000354 DAKBVG010000000
VERSION     DAKBVG010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.364"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            69..>956
                     /gene="bsh"
                     /locus_tag="OUD09_002746"
     CDS             69..>956
                     /gene="bsh"
                     /locus_tag="OUD09_002746"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HCT9693942.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTITPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITA"
BASE COUNT          330 a          128 c          178 g          320 t
ORIGIN      
        1 cttttggaat gaggaaaact ttggagtaat atgaacgtgg acagaaaaat atttaggagg
       61 aataaactat gtgtacgtct attacttatg taacaagtga tcattatttt ggaaggaatt
      121 ttgattatga aatatcttac aatgaagtag tcactattac tccaagaaat tataagttga
      181 attttcgaaa ggtaaatgat ttggatactc attatgcaat gattggtatt gccgctggta
      241 tagctgacta ccctctttat tacgatgcga caaatgaaaa aggattgagt atggccggac
      301 taaatttttc tgggtatgct gattataaag aaatacaaga agggaaagac aatgtatctc
      361 cttttgaatt tattccttgg attttaggac aatgctcaac agtaggagaa gctaaaaaat
      421 tgttaaaaaa tatcaattta gcaaatataa attatagtga cgaacttcct ttatcccctt
      481 tacattggct attagctgat aaagaaaaat caattgtcat tgaaagtatg aaagatggac
      541 ttcatatata tgataaccct gtgggcgttc ttaccaataa tccttcattt gactatcaat
      601 tatttaattt aaacaattat cgtgtcttat cgagtgaaac tcctaaaaat aatttttcaa
      661 atcaaataag tttgaatgcc tatagccgcg gtatgggagg gataggcttg cctggagatt
      721 tatcctcagt atctcgtttt gttaaagcga cttttacgaa gctgaattct gtatctggag
      781 attcagagtc agaaagtatt agtcaatttt tccatatctt aggttcagta gaacaacaaa
      841 aaggtttgtg tgatgttggt gatggaaaat atgaatatac aatttattct tcttgttgca
      901 atgttgacaa aggaatctat tattatcgaa catatgaaga cagtcaaatt actgca
//
LOCUS       DAKBVG010000355          955 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.365, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000355 DAKBVG010000000
VERSION     DAKBVG010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..955
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.365"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            218..451
                     /locus_tag="OUD09_002747"
     CDS             218..451
                     /locus_tag="OUD09_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HCT9693943.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            448..855
                     /locus_tag="OUD09_002748"
     CDS             448..855
                     /locus_tag="OUD09_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HCT9693944.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
BASE COUNT          371 a          126 c          170 g          288 t
ORIGIN      
        1 agcatataaa caggtatacg tttaataatt taaaataaat ggtatacata aatagggaag
       61 gaaatagaag ggagcaatct aaattttttg aattttctta attaattata tagaaagaac
      121 aatctttcta tagagttaga catgagaggg actatgcttg atttaaaatc aacaagcgta
      181 tatactgtat atacaaagaa aaaggaggga gaagactatg gcaattaaag aaagaaaatt
      241 tagggaagtg gggaactcag tagtattaac tatttcaaag gagtttctta aagaaaatgg
      301 gttaaatccc ggagatacgg tttttttaga tgaagaaaaa cttcgagcgg caattacaaa
      361 aaaacaagcc ttatctgatg aaattgattt agcaatatct caagcattaa ctgaatatga
      421 tactactttt agagagcttg tcgataaatg agtattcatt acctagaagc caaagatatt
      481 ataaaaatga atgtaatcca aataaaaaaa tactctccta atgaacccct tggtgtaaag
      541 gatgcgaatg ctttagaaat gtgtgtaggg caacttcgag cctcagcttt tggtgaagag
      601 gtttatcctt cgatttatga aaaagcagct atcctactta tacaattgat aaagaaacat
      661 ccttttcaca atgcaaataa acgtactgct ttcttagcta cttttgtctt tttgaaaatc
      721 aatggacagc ttttagctat tgatcaaaaa gatgctgtaa atttagcggt atatattgct
      781 acgtatgata aggatttcga tcaactaaaa tatgaagtga tcaacactat aaagacacat
      841 acgcagccca tttaaggagt tactgtatgt gtctttatag ggttattagc tgctatagcg
      901 aaataattga agtaaattac aaatagctat aattcaaatc caactatata gaaaa
//
LOCUS       DAKBVG010000356          941 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.366, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000356 DAKBVG010000000
VERSION     DAKBVG010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..941
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.366"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(22..900)
                     /locus_tag="OUD09_002749"
     CDS             complement(22..900)
                     /locus_tag="OUD09_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HCT9693945.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
BASE COUNT          285 a          185 c          155 g          316 t
ORIGIN      
        1 aactatttcg aagtactaaa cttatgaagt aagaatacct aagccagagc caatgtgctc
       61 gcctgcaagc aattgctctt ttgcttgtcg ctgtaaactc gtactttctg gataatcatg
      121 tatttgggca tctactcgtc caaagatcca agggactcca ctatcgtcct gttcatcaac
      181 tgataataat cgataatcgg ctgcaaaaag acagattttt gactcttttt tcaaccattt
      241 agtaagattg ggtgatagta accaagtatc tgtgtacatc acttggtaat ggtaatcagg
      301 aaaataaagt tcaaaaaaac gaatggcact ttcgtaacta tctcttatct tattctgaga
      361 taaatctgca tcggaaggaa tatgaagaga aaggcactgc tttccttcta atcttagatc
      421 cgtgtaccct ttcggaaaat atgtcatttc gtactccaat tcaccaatcc gaaagatcaa
      481 gccactagtg taatgccaaa tccaaaaacc acggtcaaag cgatagcttc ctgttacttg
      541 gaaatgttca tgaataaatc tagtaaaagc attcattgtt gccaaataga tagttcttgg
      601 aatcttcaat ggttgataaa tttttttcca actttgagaa gctgcatata gataaatagc
      661 taatgctgca aaaggttcct cttcatagac tttgtctatt ttctttgcag tcgcctttgc
      721 tttattagga tccattagtt catgactata ttgctcaaac acctcatcaa ggatgttttt
      781 tgcttgtaca atcatttgct ctataacgac tttgggaaga gttaaagttt ctgccagaat
      841 ttggatttca ttgcttattt tcttcatcaa atttcccctt ttcatatctt tgaacctcat
      901 tgtttaaatg actgattatc cggacaccat atggtataaa c
//
LOCUS       DAKBVG010000357          938 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.367, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000357 DAKBVG010000000
VERSION     DAKBVG010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..938
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.367"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            33..248
                     /locus_tag="OUD09_002750"
     CDS             33..248
                     /locus_tag="OUD09_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693946.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(436..723)
                     /locus_tag="OUD09_002751"
                     /pseudo
     CDS             complement(436..723)
                     /locus_tag="OUD09_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          318 a          137 c          163 g          320 t
ORIGIN      
        1 aagtagtcta tgagaggaga atcttcattc ttatgaacgc tggaattgcg gatatgaatt
       61 taataaaaaa aacccttaat gattttactt ccaattcaat atcaaaaggt actggtatca
      121 atttaagcac aataaaaaag ctgaaaagtg gtgaaagatc agtggaaaag ctgaatcttt
      181 tagatgctat aaaaataacg gaatttgcta tgaaaaatgg aaaagctgag attgaaatat
      241 ggagataatt atcgtctagt gaactacttg ccacttagct gaatttaacg atccttagtg
      301 cttgaagtgg gagattcttg ggaatagagc atacttgcaa gcttatgtct ttattaacag
      361 cgatatctct tattaaccaa gctatccccg tagttctaag gtttttactt ttatctaagc
      421 taatgatcgt attcaaagga aatttttgac ccttagaaag tctcatttta tcagacatga
      481 gcttaggcat tgctgaattt tgttaattcg gttttaggtt gtgctttgag cataatatga
      541 aagtagtctt tatcacgatt tcactcaact aaggtaatgt gatgtgattc tgatattctt
      601 tcaaagatag ttttttgcat aagctaacat tacatcatca atgagatgtt tacaatgttt
      661 cactactaaa ataaggtggt aacaaagaag aaataatgaa tgattatttg tatctaattc
      721 catggtatat caaccatttc ttatatagac tgattatgac atggattaaa ataaaaaggc
      781 actatgtctt aattgtcggt tttcgagtta ccgaacggca ttcatcaccc acctatagag
      841 gatgggcgac ttctgcctga aataagttaa actacttata acttctcata aatttagatt
      901 gagtcattat tatttaacat caatgatttt ccagtttc
//
LOCUS       DAKBVG010000358          899 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.369, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000358 DAKBVG010000000
VERSION     DAKBVG010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..899
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.369"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            133..558
                     /locus_tag="OUD09_002752"
     CDS             133..558
                     /locus_tag="OUD09_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693947.1"
                     /translation="MIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMS
                     MPASSSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRE
                     RMYEKGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT          346 a           94 c          196 g          263 t
ORIGIN      
        1 aatttatatg gaaaaacatt gacggagtta tatgagacac ttgatgaaac agaaagaaga
       61 atccataaag aactggtggt gatgaaacaa aaacaaaaag agataaggga gcaaaaaaaa
      121 cagttgaaat tgatgatcga tacaaacgag gaagaatttc ctgtagaaga gatccctttt
      181 gactgtatga tctcaacgga gtttgaagat attgtggaaa tcaaaaaatt tctacctaac
      241 tattcaagct ttgggatgat gagtatgcca gcttccagtt cacagacgat gtatggattt
      301 tttatcgacc ctagtgaagt acatttattt actcaagacg tgatttggga gaaaaaagat
      361 acggctgttt atcgtcgatt cttattgaaa tccgagatga accatgcgga acgtaataat
      421 attttagaaa tcagagaaag aatgtatgaa aaaggatgga aaacaggaga agtgatcggc
      481 cagtatctat tgacgaatac ggatgaaaac aatatcagaa ctgaatatta tcatgcatgg
      541 atagaaatga aaaaatagga aatatgtgtt taagataact cgaaaatgat ggttgtttta
      601 gttataaaga aaaattttct ggttcgatta cgggtttaat gtgggggatt attagtcgtg
      661 gatagataga attttagttt tttagaggaa atatgtcttt aaaaatggaa tggttgcaat
      721 tcttagcgat atatacgatg taattgtcct cttaaaaaag tatggaaaaa taattgtatt
      781 gggtgttatg tataattcat ggtgaactga gactaatgag aagaaaaatt catattgtta
      841 tgagtgagat tgtatcagat gagaatgata aattaaatag gtgcataaaa gcattacac
//
LOCUS       DAKBVG010000359          898 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.370, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000359 DAKBVG010000000
VERSION     DAKBVG010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..898
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.370"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            173..442
                     /locus_tag="OUD09_002753"
     CDS             173..442
                     /locus_tag="OUD09_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCT9693948.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            435..692
                     /locus_tag="OUD09_002754"
     CDS             435..692
                     /locus_tag="OUD09_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HCT9693949.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
BASE COUNT          342 a          116 c          168 g          272 t
ORIGIN      
        1 caagaaaaag caggatttga ggtaccagca ctcaaaaagt gacgtcactt tttatcctaa
       61 aaactaaaag tgatagcact tttaattata agaagttaga atattaatca tttgcttaat
      121 tgtacaatat aatgtacaat tgttttatag aaataaataa ggggtgaaag gaatggaagc
      181 agtagcttat tcaaatttcc gccaaaattt acgtagttat atgaaacaag ttaatgagga
      241 tgctgaaaca cttattgtaa caagtaaaga tgtagaagat acagttgttg tattatcaaa
      301 aagagattat gattctatgc aagaaacgtt gagaacactt tctaataatt acgtcatgga
      361 aaaaattcgt cgaggagatg aacaattctc caaaggtgca tttaaaacac atgacttaat
      421 cgaggttgaa tctgatgatt aaggcttggt ctgatgatgc ttgggatgat tatctttatt
      481 ggcatgagca aggaaacaaa agcaatataa aaaagattaa caagttaata aaagatatcg
      541 atcgttcccc ctttgctgga ttaggaaaac ctgagccatt aaagcatgat ttatctggaa
      601 aatggtccag aagaattaca gatgaacata gactgatata tagagttgaa aatgaaacga
      661 tatttattta ttctgcaaaa gatcactatt aaccaatcgg aagtaaggaa agggtcagaa
      721 acttaaaagt ttttgatcct ttattttcaa gtctgatttg gcagcttttc ggcagctcat
      781 ttatttatgg tcagaaatag atgctgaaat tttaatctat tagcaaatag tccagaacgt
      841 atgaagagga gtcggatatc atttgtcatt aacaccataa atatttataa aattcgtg
//
LOCUS       DAKBVG010000360          898 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.371, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000360 DAKBVG010000000
VERSION     DAKBVG010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..898
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.371"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            <1..866
                     /locus_tag="OUD09_002755"
     CDS             <1..866
                     /locus_tag="OUD09_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HCT9693950.1"
                     /translation="WGKEKKLTAKKDFTYIETNNPEDNSTNIDQAIRNGFTTIISLSY
                     LQAEELENAAKDNPKLKFALVDGEITGKKNISSATFKDQESAYLAGLAAAYTTKSDQV
                     GFIGGMEGPVIQRFQSGFEAGVKAGATALNKEITVNVQYAASYDSLDKGKAIAASMYK
                     EGVDILYQAAGSTGAGVFQEAKARNENDYSRKVWVIGVDRDQADEGDYKMENGETGNF
                     TLTSTKKNVGAEVKEFAETSHNDKFPGGKHLTFGLKEEGVDLTKGQLSEEAYQAVSDA
                     KEKIISGDFRL"
BASE COUNT          317 a          150 c          225 g          206 t
ORIGIN      
        1 cttggggaaa agagaaaaag ctgacagcaa aaaaagattt tacgtatata gaaacgaaca
       61 atccagagga taacagtacc aatatcgatc aggcaatcag aaacggcttt accaccatta
      121 ttagcttgag ctacttgcaa gcagaggaac tggaaaatgc tgctaaagat aatccaaagt
      181 tgaaatttgc cttggtagat ggtgagatta ctgggaaaaa gaatatctca tcagcaacct
      241 ttaaagatca agaaagtgcg tatttagcag gactagctgc cgcatatacg acaaaaagtg
      301 atcaagtagg ctttattggt ggaatggaag ggcccgtgat ccaacgtttc caaagcggat
      361 ttgaagctgg cgttaaggca ggggccacag cactgaacaa ggaaattact gttaacgtac
      421 aatatgccgc atcctatgat tcgctagata aaggaaaagc tattgctgct tctatgtaca
      481 aagagggagt cgatattctt tatcaggcgg caggaagtac tggagctggg gtgttccaag
      541 aagcaaaagc ccgcaatgaa aacgactata gcagaaaagt atgggtgatt ggtgtagatc
      601 gcgatcaagc ggatgaaggg gattataaaa tggagaacgg cgagacaggg aatttcacgt
      661 tgacttctac aaagaaaaat gtcggtgcag aagtcaaaga atttgcagaa acttctcata
      721 acgataaatt tcctggcggc aagcatttga cctttggttt gaaagaagaa ggggtagatc
      781 tgacaaaagg acaattatca gaggaagcct atcaggcagt ttcagacgca aaagagaaaa
      841 tcatcagtgg tgatttcaga ctgtagacaa agtccatttt atggatgatg tctacagt
//
LOCUS       DAKBVG010000361          891 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.372, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000361 DAKBVG010000000
VERSION     DAKBVG010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.372"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(<1..866)
                     /locus_tag="OUD09_002756"
     CDS             complement(<1..866)
                     /locus_tag="OUD09_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCT9693951.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKK"
BASE COUNT          303 a          104 c          155 g          329 t
ORIGIN      
        1 tattttttaa aagctgaaga aaaatgatgg atcgaatgaa atcctaatat atctgcaatt
       61 tctgtatagg ttaattcatt ttctagcacg agacgttttg ctttttccat ttttagttct
      121 ttaaaatagc taataacact ttttccattc acttttctaa aatgtttttt taagctggct
      181 ggactgataa aaaaaatttg ggctaattgt tcaagcaaaa aattttctga aagatgttct
      241 tctaaatatc gtatgattgg atcaactaat tcatctgaat aaagaatatg ctttttttta
      301 ggaatgactt tctcatttga aggtattgtc aataaagcaa gttcctgttg ataatgcctt
      361 ggtaaggaag acaattgaat tttttgcaga caataagtaa ctaataagga aagcaaatga
      421 atgctcatct gttggcatag tatgaaaaaa gaattgattt cctgatcata ggaaatagct
      481 ggcttactat gttttagata atcaattttg aactgttcat ttttcagaaa agtaatgata
      541 gatgttattt cttgatctat agaaaaagat tgtcccgtaa tatgtgctaa taattgctca
      601 tcaaaataaa actggataat gataaaagtt tgtgtttcct tttgagtaga aaagatttgg
      661 acttcttgct gtttatcaat gaataaaagg gaaggattat ctagttctat agatttatgt
      721 gcaaattcag catataaatt agcgctttca tttattagta gtcgaaaata gggagcagca
      781 gataaatgaa gatgggtatc tatggaatag gttagtgttt ttttgacaag attggaggat
      841 tggttgtttg ttaacaaaag aaccactttc ctttctcttt ttatataaaa a
//
LOCUS       DAKBVG010000362          890 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.373, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000362 DAKBVG010000000
VERSION     DAKBVG010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.373"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            <1..888
                     /locus_tag="OUD09_002757"
     CDS             <1..888
                     /locus_tag="OUD09_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HCT9693952.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT          386 a          116 c          144 g          244 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt
//
LOCUS       DAKBVG010000363          890 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.374, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000363 DAKBVG010000000
VERSION     DAKBVG010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.374"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(62..280)
                     /locus_tag="OUD09_002758"
                     /pseudo
     CDS             complement(62..280)
                     /locus_tag="OUD09_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(546..740)
                     /locus_tag="OUD09_002759"
     CDS             complement(546..740)
                     /locus_tag="OUD09_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693953.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
BASE COUNT          308 a          152 c          139 g          291 t
ORIGIN      
        1 taggtatttt tattatatat agatttaaca tatgtcggtc ggttgattga tatagctaaa
       61 gttataactc aaccgaagcc gattcatcta cacgaataaa gttgcgagtg ttctcggcta
      121 attcataaaa attgttatgt gttccatata gcaatactgg aataacttta ttttcttgaa
      181 tctcataagc aactctatac acggtgaccg catacttaaa tccattagac caattacctg
      241 ataaacctcc agttttttgc tcttacttaa atggatcaat ggcaatttca tcacaaacct
      301 atagaggatg agtgacttct gcctaattac gttaaaaaat attgaaagta actctatgta
      361 gcaaaaaata tctagaattt catttcctca aaactgatcg atattgtatc taaaaatata
      421 tactacctaa aataggtagt atatattaaa atatgtgctt ttgttagaaa aatcaataca
      481 taaattatca tatctgtata tgtcatttag gtatttcagt tatgttataa tagttttgct
      541 gtctgctacc ccggcctaat ctggcaaggg gaggaggaga tataatgaaa tacctttttg
      601 attttgtttt tgcaatcaag ccacttgcag atgtagtgtg cagttacctc tgcaaatggc
      661 ttgattgcaa aattgccaaa aaagacagta agcctacccg ttaattctat aatatagaaa
      721 cgtaaaaaaa accccatcag cctgcacgct gatggggttt ggtgatataa tgaaatacct
      781 ctttgttact cataattata ccataattag tcacgaatgc aaccaataga aaaaactact
      841 tagaacggta gatcatcgct aataacaatg cattgtccac caaatggatt
//
LOCUS       DAKBVG010000364          889 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.375, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000364 DAKBVG010000000
VERSION     DAKBVG010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..889
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.375"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            283..522
                     /locus_tag="OUD09_002760"
     CDS             283..522
                     /locus_tag="OUD09_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693954.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT          297 a          165 c          132 g          295 t
ORIGIN      
        1 ctatcctcct agcaactatt taaataccca aaaaaattac ctctagataa tatcatacct
       61 acgttttcac ttataaattt tttcataaaa aaagcgttta gaggcataat cgttaataca
      121 gatggtaacc aatgtatctg gtttgaactg ggatatcacc ctctccatat atgtattact
      181 aagactaata gatactataa aagacttgat agttttgcat tgattttgcc taatactgag
      241 cataggtgta tgctaaacga tactataagg agtgtgtaca taatgaaaag aattctgttt
      301 atcagtttat caaatttatt gttacttact gcttgtggga cttctgcatc aaagaacata
      361 ccttcagata cgcaaatttc gaatagtatc gattcaacaa tttctacaaa tgagaaaaat
      421 tctccggtag aaacttccga tataaaagtt tctacctctg caaaaaacag ggtaaagaaa
      481 ctgtcagcac gtctccttta tttcaaacaa actctgaatt agcttcttca tcttcttcag
      541 caaataatga tgatagtttc gcgtttactc ctaactaaat gcaaccttta caaatgcttt
      601 acgataacaa gcctgagcta aatgatcctg atatcagttt tgcatttttt gaaatgattg
      661 gatcggatga tctttttaaa actactagtt tgtctatccg aaaataggac agttctggca
      721 gtataggttt ttatcgagtt tctccaccag gcctcgttac agagacagat tctctaggcg
      781 aaccttttga ataaatcaaa aatcatgaac aatcactgcc aactaaataa agtgccttca
      841 aaagcttggt cttctcgctt ttgaaggcac tttatttaaa tctttatga
//
LOCUS       DAKBVG010000365          877 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.376, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000365 DAKBVG010000000
VERSION     DAKBVG010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.376"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            <1..>877
                     /locus_tag="OUD09_002761"
     CDS             <1..>877
                     /locus_tag="OUD09_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCT9693955.1"
                     /translation="SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHY
                     PKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIR
                     AYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDS
                     IEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEY
                     SYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISEC
                     TIDNKQNVLEEYILLR"
BASE COUNT          366 a           77 c          146 g          288 t
ORIGIN      
        1 tcattaaaaa aacattatac agagccttgg gaagatgaag tttttagagt aattattgaa
       61 tataacaatg ttcctattgg atatggacaa atatataaaa tgtatgatga gttatatact
      121 gattatcatt atccaaaaac tgatgagata gtctatggta tggatcaatt tataggagag
      181 ccaaattatt ggagtaaagg aattggtaca agatatatta aattgatttt tgaatttttg
      241 aaaaaagaaa gaaatgctaa tgcagttatt ttagaccctc ataaaaataa tccaagagca
      301 ataagggcat accaaaaatc tggttttaga attattgaag atttgccaga acatgaatta
      361 cacgagggca aaaaagaaga ttgttattta atggaatata gatatgatga taatgccaca
      421 aatgttaagg caatgaaata tttaattgag cattactttg ataatttcaa agtagatagt
      481 attgaaataa tcggtagtgg ttatgatagt gtggcatatt tagttaataa tgaatacatt
      541 tttaaaacaa aatttagtac taataagaaa aaaggttatg caaaagaaaa agcaatatat
      601 aattttttaa atacaaattt agaaactaat gtaaaaattc ctaatattga atattcgtat
      661 attagtgatg aattatctat actaggttat aaagaaatta aaggaacttt tttaacacca
      721 gaaatttatt ctactatgtc agaagaagaa caaaatttgt taaaacgaga tattgccagt
      781 tttttaagac aaatgcacgg tttagattat acagatatta gtgaatgtac tattgataat
      841 aaacaaaatg tattagaaga gtatatattg ttgcgtg
//
LOCUS       DAKBVG010000366          863 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.379, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000366 DAKBVG010000000
VERSION     DAKBVG010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.379"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            333..>863
                     /locus_tag="OUD09_002762"
     CDS             333..>863
                     /locus_tag="OUD09_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693956.1"
                     /translation="MRSDSRFFVSNLQDDELIRQIDSLLETITESDKRIFLNYVELTR
                     HIIELDKLFNVFRYNLTNLLKHFTIFTNDLIESTGEKLTEDQYYYQINALTINLISSA
                     KTLTESIEVCMKNFLAEKDFKSFKNKILSKPYDEHFSYRFLLHVRNYSQHGHLPVNIE
                     QQRVYFDLDEILTMPHF"
BASE COUNT          327 a          103 c          130 g          303 t
ORIGIN      
        1 aatagatcca gactattatc aaccacaaga agtttttcga gagtttagaa atcagattat
       61 tcgttcaagt aatattgaaa attgagtcaa taaaagaagc gttgaagaaa agttccttct
      121 ttcgacgctt ctttttaaaa gattttacaa atgtcttatg gagatttgct tcaatgacta
      181 cttgtagtca tttcgtttaa aataattatg ttacaccctt atgtgtcaca gagaaatact
      241 gatataataa gaaataaggt atgtaacaat agagtatact tctatgttac atattgatta
      301 ttttgaaaaa atcatattag gagaatataa ttatgagaag tgatagtagg tttttcgtca
      361 gtaatttaca agatgacgag ctaataaggc agattgattc attattagaa acaataacag
      421 aatctgataa aagaatattt ttaaattatg tagaattaac acgtcatatt atagagttgg
      481 ataaactttt taatgtgttt agatataatt taactaattt gctcaaacac ttcacaattt
      541 ttactaatga tttaattgaa agtacaggtg aaaaattaac ggaagaccag tattattatc
      601 agataaatgc tttgacgata aacttaatta gttcagccaa aactttgact gaatccattg
      661 aagtttgtat gaaaaacttt ttagcagaaa aagattttaa atcttttaag aataaaattc
      721 tgagtaagcc ttatgatgaa catttctcgt acagattttt attacatgtt agaaattatt
      781 ctcagcatgg tcacttaccg gtaaatattg aacaacaaag agtatacttt gatttggatg
      841 aaattctaac aatgcctcat ttt
//
LOCUS       DAKBVG010000367          862 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.380, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000367 DAKBVG010000000
VERSION     DAKBVG010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.380"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(19..723)
                     /locus_tag="OUD09_002763"
     CDS             complement(19..723)
                     /locus_tag="OUD09_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693957.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT          241 a          161 c          151 g          309 t
ORIGIN      
        1 ttatgacaaa tagaattttt atcgatcagt aatcgctgtc gctgcaaatt gttcgatcaa
       61 aggaatcaaa gtcaatttga ctttgtattt ccgttcgagt gctttttcga taacgaactc
      121 attttcagta gtcatagaaa tagttgctaa tttacgaggt tcatcagcat ttttttcatt
      181 aataactact tcatgttcat tttgttctgt gtaaaattta ggatactcag caacggcttt
      241 tgaaaaatca gcaaaactta aagcggcatt cgtatcagtt gagcgtgtat tttcagttgt
      301 gtccgctttt ggtttttccg ttttttcttc tggcacactg actgtttctt ctgtaactgg
      361 tgtcgcaaca gcttgcgcag ttggttcatc gtttttcaat cgttgatcat tccagtaaga
      421 cacgatttca ataagaagat tgtcacggaa tcgttcgaaa atcccgtttt ctcctgaacg
      481 aattctcttt aatacagtat tgaagttgtc atcgattggc acgttgatcc gtgtatcttc
      541 atttgttttc ccagctaatc cgatcgttaa gatttgacgg tttttgggat caagcatgac
      601 ttgtaacggc aaaccatagc gtcgcaagct ctcttcttct agagaacgga ataattgtcc
      661 tcttacacca tccaatgaag gacggaattg atttaataat ttttgtttct gttttttctt
      721 catatgttct ctccttcgct catacccttt cgattataag aaaaaaacag ccaaatgaaa
      781 aggagtaatg caaaaaaact tgcgtatacg caaattacaa cagaaaaatt tgcgtatttt
      841 cccgtttttt gaaagaaaaa ta
//
LOCUS       DAKBVG010000368          861 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.381, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000368 DAKBVG010000000
VERSION     DAKBVG010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..861
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.381"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            6..860
                     /locus_tag="OUD09_002764"
     CDS             6..860
                     /locus_tag="OUD09_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCT9693958.1"
                     /translation="MENSIYLVTEPNTQLKDCYFNFCGYSKTEPRHSFGPAIREQYLI
                     HIVLEGEGYYSINDQKYFLKKGQGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYY
                     LKEIGVGKNHLTFKVENLNSFKGLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSAS
                     YLNEGIITKKMNPFVRQTIEIIRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISI
                     KEYISEIKISIANDLLSSTEYSIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRL
                     GLGIINGE"
BASE COUNT          322 a          118 c          147 g          274 t
ORIGIN      
        1 tggatatgga aaattctata tatttagtaa cagaacctaa cactcagctg aaagattgtt
       61 actttaattt ttgtgggtat tcaaaaacag aacctagaca tagttttgga ccagctattc
      121 gagaacaata cttaatacac atagtgctag aaggtgaagg ctattattca attaatgatc
      181 aaaaatattt tctgaagaaa ggccaaggat ttgttatccc accaggagta tctacttttt
      241 atcaagcaga tgaaaaaaat ccgtggtcat atgtatggtt gggtttaagt ggcaatcttg
      301 tagaatacta tctaaaagaa attggtgttg gaaagaatca tcttaccttt aaagtcgaaa
      361 atttaaattc atttaaagga ttaatttttg aaactttatc gtatgatcag gacaatctag
      421 tcagtgaatt gtccagacaa aaacaagttt ataaattttt agaactcctc ttaaattcag
      481 ctagttatct gaacgaaggg ataattacta aaaagatgaa tccttttgtt cgtcaaacaa
      541 ttgaaataat acgtgagaat gcgtatcaaa aattatctgt taaagatatt gctgtcagtc
      601 tatcgataca cgaaaattat ttatcacgat tgtttaagaa tgatatggga atatcaataa
      661 aagagtatat tagtgaaatc aaaatttcta ttgcaaacga tctgttatca tcaactgaat
      721 attcaatcca gcaaatcagt gacaagatag gattctcaac agtacaatct ttttctaaag
      781 cctttaagaa agcaaatggt gtttcaccgg gagtatatcg aaaacgtaga ttaggtctag
      841 gaatcattaa tggtgaataa a
//
LOCUS       DAKBVG010000369          860 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.382, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000369 DAKBVG010000000
VERSION     DAKBVG010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..860
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.382"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            71..832
                     /locus_tag="OUD09_002765"
     CDS             71..832
                     /locus_tag="OUD09_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCT9693959.1"
                     /translation="MHTPEEIMELSIHLGTKKIHKPILAKLILGFIGGAMISLGYLAY
                     VRVSASIPESMASLTSLVGASVFPIGLLVILLGGGELITGNMMAVAIAFMDKKVTFSQ
                     LVKNWSVITFANIIGAIFVAYFFGHFVGLTHSGVYLDQVIHIADHKIAVSWWQALGSG
                     IGCNWFVGLALWLSYGAKDAAGKVLTIWFPVMIFVAIGFQHSVANCFVIPAAIFEGQA
                     TWLEFLSNFIPVYIGNIIGGAIFVSGFYYVSYKHH"
BASE COUNT          224 a          144 c          184 g          308 t
ORIGIN      
        1 agaaaatgtg atttttttca ctatatttta tcacatgaaa attcattaag aaagtaggaa
       61 tcgaagtatt atgcacacac cagaagaaat tatggaactc agtattcatc ttggaacaaa
      121 aaaaatacat aaacctatct tagctaaact gattttaggt tttatcggag gagccatgat
      181 ttctctaggt tatttggcat acgtacgagt ctctgcttct atcccagaat ctatggctag
      241 cttgactagt ttagttggtg cctcggtttt tcctatcggc ctgctggtca tccttcttgg
      301 cggtggtgaa ttgatcaccg gaaatatgat ggctgtggcg attgccttta tggataaaaa
      361 agtcacattc tcacagctgg ttaagaattg gtcagtgatt acttttgcaa atattattgg
      421 tgcaattttc gttgcctatt tctttggaca ctttgttggt ttaactcatt caggtgtcta
      481 tctcgaccaa gtgattcata ttgctgatca taaaatcgct gtttcttggt ggcaagcatt
      541 aggctccgga ataggttgta attggtttgt cggtttagcg ttatggttat catatggagc
      601 aaaagatgcg gccggcaaag tactaacgat ttggtttcca gtcatgattt ttgtggcaat
      661 cggtttccag catagtgtgg ccaactgttt tgtgatacct gctgccatat ttgaaggtca
      721 agcgacttgg ttagaatttc tctcgaattt tatccccgtt tatattggaa atatcatcgg
      781 cggagctatt tttgtttcag gtttttatta cgttagttat aagcatcatt aagattgaag
      841 gtaatgttca ttcgagcatt
//
LOCUS       DAKBVG010000370          830 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.385, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000370 DAKBVG010000000
VERSION     DAKBVG010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..830
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.385"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(518..691)
                     /locus_tag="OUD09_002766"
     CDS             complement(518..691)
                     /locus_tag="OUD09_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693960.1"
                     /translation="MIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGV
                     LRCTAKGMGRLIG"
BASE COUNT          234 a          190 c          127 g          279 t
ORIGIN      
        1 taaaaataca taaaaaattt cgactcgttt atggcgtccg aaacgagaaa ccctagtaga
       61 cagcggtaaa tgctcgattg atccaataat caacgtcctc gcaagtatct acatgtactg
      121 cagcacacga cacaatttct tgtgcgtcct ccccattacg agaagaccta ctccttattc
      181 catgagtttg cgtcaaagtc tagatccctt agtagctgct catgattaat ctagacgcta
      241 ttctaacttg tctaacttct aaattccctc ttgtttactc tgcgcttatt atttacacaa
      301 attcatttca ccactatcca aacaagcgca tttactcgat tgaccaattt tttacgttac
      361 caatcgttaa aacctacccc gtcactcccc gaagggaatt gttgatagat caagtctact
      421 ccttattttt ctagtgactc taagttcctt tagaagtctc gacaatattt tcaagcaagc
      481 gaaaaacgat aaaatacaag ttatgagaat gatcaattca gccgatcaaa cgccccatcc
      541 cttttgcggt acaacgcaag actccatttt ttcttcgacc aatcagtaca ccgttttggt
      601 cataaacatg tttgattgtt ggatatagct cttttaaggg gataggatct tttaaaatta
      661 tttgcttttg tttcccaatg taataaatca tttcctctga gactttgtct ttctggatta
      721 tatatttctt acactgcaca acggttttac tttggccagt tgatagtttc ttcgtgattt
      781 tctctgtagc ggtacaccgt ttaccgtagt gacaaatatc tttgagactc
//
LOCUS       DAKBVG010000371          829 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.386, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000371 DAKBVG010000000
VERSION     DAKBVG010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..829
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.386"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            63..>829
                     /locus_tag="OUD09_002767"
     CDS             63..>829
                     /locus_tag="OUD09_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCT9693961.1"
                     /translation="MHKISVIVPAYNVENYLENCLESISNQTHSNIEIIVVDDGSPDR
                     SGEIAEEYKKKDMRLKVIHQVNGGLSAARNSGLDSATGDFVCFIDSDDWIEPDYLELL
                     YFGTQTYGADISVLKIKKISSLEQLKEHTIAERKWEIFDREQAMKNLFINNKIGYSAN
                     NKLFNRNLFDNIRFPLGKLMEDKATTYLLIDHSEKVAVNSSAKYHYYQSPKSILRGSF
                     NPRIFDSFEVHEDILVFIDKFYPELSINVRTRYVYEA"
BASE COUNT          315 a          104 c          153 g          257 t
ORIGIN      
        1 attgttactg gtaatgatac atttgtaaaa ataaaaaaga tgatttttag gaagtgaaga
       61 gagtgcataa aattagtgtt atagtacccg catataacgt agaaaactat ttagaaaact
      121 gcttagaatc tatttccaat cagacacatt caaatattga aataatagta gtcgatgatg
      181 gttccccgga tcgaagtggg gaaattgcag aagaatataa aaagaaagat atgcgattaa
      241 aagtgataca tcaagtgaat ggtggtttaa gtgcggctag gaattctggt cttgattctg
      301 caacaggaga ttttgtgtgc tttattgaca gcgatgattg gatagaacca gactatcttg
      361 agttactata ttttggtacg caaacttatg gagcagatat ctcggtgcta aaaataaaaa
      421 agatttcaag tttagaacaa ctaaaagaac atacaattgc tgagagaaaa tgggaaatat
      481 ttgatagaga acaagcaatg aaaaacttat ttataaataa taaaattggc tattctgcaa
      541 ataataaatt attcaataga aatttatttg ataatataag attcccactg ggtaaattaa
      601 tggaagataa ggctactact tatttgttga tcgatcattc cgaaaaagtt gctgtcaatt
      661 catctgctaa atatcactat taccaaagcc ctaaaagtat actgagagga agcttcaatc
      721 ctagaatttt tgattctttt gaagtccatg aagatatact agtattcata gataagtttt
      781 atccggaatt atcaattaat gttagaactc gctatgtcta tgaagcaat
//
LOCUS       DAKBVG010000372          822 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.387, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000372 DAKBVG010000000
VERSION     DAKBVG010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..822
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.387"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(<1..>822)
                     /locus_tag="OUD09_002768"
     CDS             complement(<1..>822)
                     /locus_tag="OUD09_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCT9693962.1"
                     /translation="LKDFAKEQFTSVSTVFRYAKLLIPYFRRYHITFHPFQLELNASE
                     ANIRSFFYYFYWNSTRESSDKWPFHIEQKEIEKYIVAFEGIYDITLTIFQKRVFSFWL
                     AINIERSSFRKVRVDNEYKSVISDDPHFNLLKKWSKQINLSFNSDELCFLYRIIYSFG
                     VIDGNAIYENSHAYAHQRQNTCSYRAVENLEKVLQSMFRFSLDIKDPELIFNFIAFHE
                     RSYLFYGNPDLFFNRSYIEEMKEEEPRTYHIMEKLKKELQANADLDVSKKLENWA"
BASE COUNT          286 a          138 c          109 g          289 t
ORIGIN      
        1 tgtgcccagt tttctaattt ctttgataca tccaaatccg catttgcttg taattctttt
       61 ttgagttttt ccattatgtg ataagttcga ggttcttctt ctttcatttc ttcgatatac
      121 gaacgattaa aaaaaagatc tggattacca taaaatagat aacttctttc atgaaatgca
      181 ataaaattga atatgagttc tggatccttt atatctaaag aaaaacggaa catgctttgt
      241 aaaactttct ccaaattctc aactgctcga taagaacaag tattttgtcg ctgatgtgca
      301 tatgcatgac tattttcata tattgcattt ccatcaatta ctccaaaaga ataaattatt
      361 cgataaagga aacacagttc atcgctgtta aaagaaagat tgatttgttt tgaccacttc
      421 ttcaaaagat taaaatgcgg atcatctgag ataactgatt tatattcatt atctactcgt
      481 acttttctaa atgaacttct ttctatatta atggctaacc aaaaagaaaa tacacgtttt
      541 tgaaaaatag ttaacgtaat atcataaatc ccctcaaaag caactatata cttctctatt
      601 tccttttgtt cgatatgaaa tggccactta tcactacttt cacgtgtaga attccaatag
      661 aaataataaa aaaagctacg aatgtttgcc tctgatgcat tcaattctaa ctgaaatgga
      721 tgaaatgtta tatgataacg cctaaaatat ggaattaaga gcttcgcata tctgaaaaca
      781 gtagatacac tagtaaattg ctctttagca aaatctttta ga
//
LOCUS       DAKBVG010000373          812 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.388, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000373 DAKBVG010000000
VERSION     DAKBVG010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.388"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            <1..622
                     /locus_tag="OUD09_002769"
     CDS             <1..622
                     /locus_tag="OUD09_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HCT9693963.1"
                     /translation="IRNLENMVFNLSNGIIPSVSGDTIRSEKNYSIIVFEKLAQTSIT
                     LGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIVYYTKEIGKTKIEHLSPLISSV
                     VQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMHITIYNYISKRKISTAKIMLKS
                     NHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLGLVDNFNNIS"
BASE COUNT          293 a           90 c          149 g          280 t
ORIGIN      
        1 tattcgaaat ttagaaaaca tggtttttaa tctgagtaat ggaataattc caagtgttag
       61 tggagatacg attcggtctg aaaaaaatta ttctatcatt gtttttgaaa agttagcaca
      121 aacgtctata acattaggaa tggatataat agaagcgtat caaagtagag atgctttgat
      181 acaagaaaac gaattagctg tatcgttacc tgaggtctta aaagtgaggg attctgggat
      241 tgtttattat actaaggaaa tcggaaaaac taagatagag catctttctc ccttaatttc
      301 ttctgtcgtt caattcatcg gcttaaatat ctataaaaga attactgtaa aggaaatagc
      361 taattatttt tctgtaagtg aaactaaatt aagagaaagc tttaagaatg aaatgcatat
      421 tacgatttat aattatatct ctaagcgaaa aatttctacg gctaaaataa tgttgaaatc
      481 aaaccatact atcagtgaag tttctttagg tttaggattt tcagattctt cacacttttc
      541 gagagtattc aaaaagtacg caggtgtatc tcctaagcaa tatcaattag gtttagtgga
      601 taatttcaac aacattagct gagaggtttt attttattat gagactatat catttacgta
      661 gtttggagga gttgaggaaa ttggatttta acaaaaaatt ttttgggaaa tttctgattt
      721 gagttgtttc gtagaatctg agcgtctaag taatggtaat aatagtgtgt taaatgaata
      781 tgacaaagta gtaattaata aagtaaaaag tt
//
LOCUS       DAKBVG010000374          800 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.389, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000374 DAKBVG010000000
VERSION     DAKBVG010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..800
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.389"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(34..528)
                     /locus_tag="OUD09_002770"
     CDS             complement(34..528)
                     /locus_tag="OUD09_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HCT9693964.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            complement(595..>800)
                     /locus_tag="OUD09_002771"
     CDS             complement(595..>800)
                     /locus_tag="OUD09_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HCT9693965.1"
                     /translation="EIQPTELADIVKTTIRSSNGMISSLNEARNRHSLAHPNTNIIGK
                     REAKLIIGISSTVTDYISGYLDK"
BASE COUNT          236 a          167 c          110 g          287 t
ORIGIN      
        1 ttattaattt aattataaca cggattgtta aacttactga caatattcaa ataccccatg
       61 agaggtaact aggtaattac cctctatttc catatcacgt cccaaagatt ggtagtcaat
      121 ataattttgt aaattggaag ggacttcacc aagctgtcct gtttcttcca cgtaataacg
      181 ggcgacatct tccatcgaat cacaatcact ataacaatga atatcctctt tgttttccag
      241 taattcttcc aagctactaa accacatgcc ttgaatttct ttgagttcat tataaatagg
      301 tgtaccttcg atttcttgaa tggcttcaca tagacggttg atttcactaa tcggcgtgta
      361 ttcgtccaca tcaaaaggaa gctcaaaatc atgtattgca tattcttcgt agtcctcatt
      421 taaaccgatt ctttctgcca tttcttcctc atcaattggt ggagtaaacc atgctccgac
      481 taattcacct tcattatacc gtcctaaatt tgccacatag actttcattt ccaattctta
      541 tcacatcctt tttataaaaa tagtcataaa aaaagtagca atcagttaat gacgctactt
      601 atcaaggtat ccgctaatat aatctgtaac tgtacttgaa atacctatta ttagttttgc
      661 ttctctcttt ccaataatat ttgtattagg gtgtgcaagt gaatgcctat ttcttgcttc
      721 attaagagag ctaatcattc catttgatga ccttatcgtt gtcttcacta tatcagcaag
      781 ttcagtaggt tgaatttctt
//
LOCUS       DAKBVG010000375          798 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.390, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000375 DAKBVG010000000
VERSION     DAKBVG010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..798
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.390"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(516..716)
                     /locus_tag="OUD09_002772"
     CDS             complement(516..716)
                     /locus_tag="OUD09_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCT9693966.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT          250 a          154 c          106 g          288 t
ORIGIN      
        1 acaaaaaata aaaaaattat tacgttatta aaaaaacaga tgaaagatca ggttttatta
       61 gatctcattc tttcatcact tgaagggatt taatgaacta ctttttcctc tttgtcgttt
      121 ttatatattc gtattccttc aaacagtagg atagcctctt tcaatgcttg atatttgaac
      181 tgtccgttac ttaaaaattt agatgtgccg ccctcttcct agaacatatt cttttttact
      241 ttttcattaa ttgacagacc aatcgaacga aactgtaaat ttcgatagga taatgattgg
      301 tcaaactcat tcattttcaa gacatctcga ttcatattca agataggaat ctttttatca
      361 acatcacttt attcgtattt tcctctacca taaaagcttc acgctccaca aaattgtttt
      421 atacactact attcagccca ttcaacgacc aatgaatttt ttcgtacaaa aaaaccgtct
      481 tttctttcga aaagacggtt aaaagtcaat ttttattaag ctttaacaat gtttgttgct
      541 tgagggccac gttgaccatc ttcaacatcg taagacactg cttggccttc ttctaaagtt
      601 ttgaagccgt caccttggat agctgaaaaa tgtgcaaata catcatttcc atcttcacca
      661 gtgataaagc caaaaccttt gtctgcgttg aaccatttta ctgtaccgtt attcataaat
      721 aaattcctcc tgatacgtat caatgtacgt tttttcgttg caataaatat ttgataggca
      781 aaaggagttt tatctatg
//
LOCUS       DAKBVG010000376          794 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.391, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000376 DAKBVG010000000
VERSION     DAKBVG010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..794
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.391"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            61..762
                     /locus_tag="OUD09_002773"
     CDS             61..762
                     /locus_tag="OUD09_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCT9693967.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
BASE COUNT          286 a          146 c          138 g          224 t
ORIGIN      
        1 tacaaattct ttaaaaaaag tgacaataaa tatagtgaac aataaggaga ggacttttta
       61 atgcgtgtta ataaaatcga ttttcacttt tcaaaactca gatatcaacc tgattttgaa
      121 catttctcgg atgaagagtt ccaacagatc aaggaacata ctgttcttcg ttcttataaa
      181 aaaggacaga tccttttcga cgatggtgat cccagacaac ggttgtatat cttgaccaaa
      241 ggactcgttc gtttagaacg ttatgatgaa agtgcaactt attactacta tgactatgtg
      301 aatacaaata acctttttcc tatgtcagga atgtttggag aagagaccta cacttataca
      361 gctcaagcga tgactgatat tgaaacattc taccttcctg tttcctacta tgaaaaatta
      421 gcacgaaaaa atcccgaaca gttgatctat atgatacaaa aaatttctaa agtagttgaa
      481 atacatgaat tgcgtatcca aatcggattg acctctagcg cctttgaccg ggtaaaacaa
      541 aatttattca ttttgaaaga agaattgggc gtaccaacaa aagatggtca tatcaccatt
      601 ccttatccga tcaccttaaa agaattagca gtgaatagcg gcacgacacg tgaaactgct
      661 ggacaagtaa tcaaacaact acgtgattcc gggctgttag attatgacaa aaagcgtttt
      721 actttctgta agacagctac tactccagct atgagagaat aacgcgaaca aaaataagca
      781 gtgaccctga taaa
//
LOCUS       DAKBVG010000377          766 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.392, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000377 DAKBVG010000000
VERSION     DAKBVG010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..766
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.392"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(109..>766)
                     /locus_tag="OUD09_002774"
     CDS             complement(109..>766)
                     /locus_tag="OUD09_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HCT9693968.1"
                     /translation="IGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQP
                     LEAIVTDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTD
                     VKLFHTDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQ
                     FETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA
                     "
BASE COUNT          213 a          175 c          146 g          232 t
ORIGIN      
        1 gacaaaaatt ataaggtgtt gtgaatgctg cggtgttatg gctacgattg cccttgacaa
       61 tgagcatcct cggatctgat tttctccggc agaagcacaa tcattcaatt acgctgcctc
      121 tccagaggta tcagatccgc gctcattatc aaggattcgc ttcgccaatc tctgttgtcg
      181 aatttggatc ggtgtttcat acgccaacgt gccatgtaag cgtagatagt tccaccaatg
      241 cacataatca aacaattcta gacgtagctg tgccagtgtc tcaaattggt attgatgcac
      301 gaattctact ttgacagatt tataggttga ttccacaacg gcattatcat acggacaacc
      361 cttcctactc aatgaacgag taattccaaa accattcaga atctcatgaa tggtttggtt
      421 atcaaattcc tttccccggt ctgtatgaaa aagcttgacg tctgttaaag aatacggtat
      481 ccgtccaaag gcttctttta ccaatgaggc atctttcttc tcaccacagg aataaccaat
      541 aatttctcga ttaaacaaat caagtattaa gcagatataa tgccactttt tccccacgcg
      601 aacataagta agatccgtaa cgatggcttc caatggctgt tcttgtgtaa aggtccgatc
      661 taatacattc gctgttttcg cttcattaca agctgttcgt tgccctttaa aatgagcgat
      721 cgtataggta gatgtcaatc cgcggcgttt catgattcga ccgatt
//
LOCUS       DAKBVG010000378          748 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.393, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000378 DAKBVG010000000
VERSION     DAKBVG010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.393"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
BASE COUNT          258 a          123 c          123 g          244 t
ORIGIN      
        1 ctttaagcgt taagaactgt taagtttagt taagtgagga tagttcacaa aatccattgt
       61 taaacttcac gaaagtggta aaactcaaaa tgctttatat aggattacag catcgctctt
      121 tcttcgattg cgcgttgggt aaaaaatatt atgaagtaaa aattgacgat gacacgttcc
      181 ttacggctca tcagatgaaa cagctacaaa aacgaaatga tcaactcgaa gaaaatttaa
      241 ttctaaaaaa gcgattgcca tattcacgcc tcgctcgacg aactattaaa ggtcaagatt
      301 tacctgaaga tcatcctttt taggaaattg aagcttttta tgttgcacaa tgcatttata
      361 atactacaat gatgttttca ccagatattc ttctagggtg cggtgtaatg aaacagatac
      421 acttgcaaaa gaaaattcac cgcgaattta ctaaaattat gaataattat gttaatcttc
      481 cgaaattttt ttccaaaatt aagggatcaa gctggtgtta tagattgttt aaggttggca
      541 aaaaaataac ttctttttaa acaattatag aaaaccgata ctctgttaag ttggttgaca
      601 cggtgtcgat ttttatgact ttcttattct gaaacttcac taacggacag tacctaaaaa
      661 gtctatagta tggacgttta cgagtactat tttatcaaaa atgctgctca tttcaactta
      721 tatgttttag tgaactaccc ccacttag
//
LOCUS       DAKBVG010000379          731 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.394, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000379 DAKBVG010000000
VERSION     DAKBVG010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.394"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(138..491)
                     /gene="tnpB"
                     /locus_tag="OUD09_002775"
     CDS             complement(138..491)
                     /gene="tnpB"
                     /locus_tag="OUD09_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCT9693969.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(481..675)
                     /locus_tag="OUD09_002776"
     CDS             complement(481..675)
                     /locus_tag="OUD09_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693970.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          220 a          156 c          122 g          233 t
ORIGIN      
        1 ttgaagcaac tgaagcaacg tatctgtagt ttccaaaatt tttcaccacc ttgaatcgaa
       61 ctgattttgt tacagaatac cacaagaaac gcacaagaaa taggggttca acacattttt
      121 tgtgttaacc ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga
      181 tcgatactta atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg
      241 tctttcgtcc gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa
      301 ccatcaccgt cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa
      361 acggcatctt cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca
      421 attccttttc gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa
      481 tcataaagca ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc
      541 gttatagatc aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc
      601 cgcgggttta cctttttctg gtgcactctt ttcgtttcct aattggacag gaacaatctc
      661 atttaattta tacatagaaa acctccagac ttcttgatag ctcaagtata tcttgaggtg
      721 catttgttag t
//
LOCUS       DAKBVG010000380          728 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.395, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000380 DAKBVG010000000
VERSION     DAKBVG010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..728
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.395"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            <1..717
                     /locus_tag="OUD09_002777"
     CDS             <1..717
                     /locus_tag="OUD09_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCT9693971.1"
                     /translation="TFTGMIFKFANRRYGLKYNPVNGVSLARKPKTNEEIKKEKDGYL
                     SKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGELISLTESDYDGNKITVSGTYD
                     YELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKLIKNNKDKYIFISKNGNPISIQ
                     AYNQSLRAVSKELNIDKKVSSHMLRHSHISLLTELGIPLKAIMDRVGHEDSKTTLKIY
                     THTTKNMQNQLVEKLGKIEI"
BASE COUNT          310 a          102 c           97 g          219 t
ORIGIN      
        1 acttttacag gaatgatttt taaattcgcc aaccgtcgat atggattaaa atataatccc
       61 gtaaatgggg tttctttagc aagaaaacct aagacaaacg aagaaataaa aaaagaaaaa
      121 gatggttatt tatcaaaaga agaagttcaa atgattgcta gtaaacaaaa aagttcacat
      181 caacagtcac gttattcttt aattactaaa tttttgtttt taactggact acgttatggt
      241 gaattgatat ctttgactga atctgattat gatggtaata aaattacggt ttctggtact
      301 tatgattacg agttaaaaat taaaacaaca actaaaaata caggttctta tagaaccatt
      361 gaattatcaa gtaatgctaa agaaatcata gatcaattaa ttgaagaaaa caaattaatt
      421 aagaataata aagataaata cattttcatc tctaaaaatg gtaacccgat atctattcaa
      481 gcatataatc aatctttaag agcagtatcc aaagaattaa acatagataa aaaagtttct
      541 tctcacatgt taaggcattc ccacatttct ctacttacag aattaggaat cccattaaaa
      601 gccattatgg atcgtgtagg acatgaagat tcaaaaacaa cattaaagat ttatactcat
      661 acaactaaaa atatgcaaaa tcaactagta gaaaaactag gaaaaataga aatttaaatt
      721 gccccttt
//
LOCUS       DAKBVG010000381          727 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.396, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000381 DAKBVG010000000
VERSION     DAKBVG010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..727
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.396"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            <1..382
                     /locus_tag="OUD09_002778"
     CDS             <1..382
                     /locus_tag="OUD09_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693972.1"
                     /translation="GISVTGTTIPTLADSTNEPTTVVDNNQELTPLDRNGIAIGNPTN
                     DLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSRNWKEKRNGYWYYGK
                     LYLQSYKSAGNNWIATYSGTLTRK"
     gene            392..595
                     /locus_tag="OUD09_002779"
     CDS             392..595
                     /locus_tag="OUD09_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693973.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
BASE COUNT          284 a          113 c          112 g          218 t
ORIGIN      
        1 tggaattagt gtaactggaa ctacaattcc tacacttgct gatagtacaa atgaaccaac
       61 tactgtagta gataataacc aagaattaac tcctttagat aggaacggaa tagccattgg
      121 taacccaact aacgacttaa ctgttactga aaattattta atagtttctc cattacttgc
      181 tcgagcagct tctaaaacga tacgagtgac aggaacgatg atttttgata aagttggttg
      241 gccttctaga cctcctcaat cacggaattg gaaagaaaaa agaaatggtt attggtacta
      301 tggaaaattg tatttacaaa gttataaatc agctggaaac aattggattg cgacttattc
      361 aggaacctta actagaaagt agggattttt tatgccagaa tttgtgaaca caccaaattt
      421 ttatcaagat agcgatattc taagtaaaat taatttaccg aaagaagaat cagctttaca
      481 agttcagcga actttacagg agacacaaaa aattcaaaaa tcaatttcta atctagaaga
      541 ttctgaagaa aaaagaaaaa aaagagacca aaaaacatat caaaaagaaa aataatgatt
      601 ctaaaaaaag agtgtctagt cgacattctt tttttctaat tgaaagtgtt cataaaatca
      661 cccatttctg gacactttct ttttttgctt tatttttcaa aaaaacaatt catttcatgt
      721 tcataaa
//
LOCUS       DAKBVG010000382          714 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.397, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000382 DAKBVG010000000
VERSION     DAKBVG010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.397"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            94..462
                     /locus_tag="OUD09_002780"
     CDS             94..462
                     /locus_tag="OUD09_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HCT9693974.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
BASE COUNT          255 a          117 c          109 g          233 t
ORIGIN      
        1 gttacaacat tacttgtgtt ttgttcgttt tttatacata actattgttt ttctggtaaa
       61 ataaatcaaa ccacttaatg gaggcgtttt agaatggaaa aatttcagcc gatcatcaat
      121 cagatcaaag aaaaagaaca tcaacaaaag atcaaagacc tttttgctaa gataacagaa
      181 acgtatcccc agctagacac aactgtaaaa tggaaccaac ctatgtttac tgaccatggt
      241 acctttatta ttgcatttag ttttgctaaa aatcattttt ctatcgcacc ggaaaaagca
      301 gcgattcgtg cccttgaaaa aaatatccaa gaagcaggct acgtatatac agataacgtc
      361 atcaaagttc cttggaaaag tgtaatcaac tgggaattga ttgaacaatt gatttcattt
      421 aatattgaag ataaaaaagg tcatgataaa ttttggcgat aaagctttat acgaataaac
      481 cttttactgg cgaggaaact attcatcagc ttatacaggt tttgttcaat cctatccttt
      541 ttatcatccc tggttaaaca cttaattgac aaagtatctg ctatttttat aaggagtctt
      601 tcttgtgctg aaaaaaatca ctgattgaat ggctttgaat tttccactta gtaaaattat
      661 tttttcagca attttcctgc aaaacttttg aataaggaaa aaatacgaga aaat
//
LOCUS       DAKBVG010000383          708 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.398, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000383 DAKBVG010000000
VERSION     DAKBVG010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..708
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.398"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(76..297)
                     /locus_tag="OUD09_002781"
     CDS             complement(76..297)
                     /locus_tag="OUD09_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693975.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(395..640)
                     /locus_tag="OUD09_002782"
     CDS             complement(395..640)
                     /locus_tag="OUD09_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693976.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
BASE COUNT          220 a          131 c           96 g          261 t
ORIGIN      
        1 tgaaatttat tgtaatatga agctgtattt tacatatagt aaccaaagag ttaataaaat
       61 aaaaagagcg aaccattata gatcagctct tttttcattg ctgtttttaa tcttatctag
      121 aatcacttca aatgtttcaa aggtatttac atctaactga cataaccaaa gcaagtactt
      181 cttttctttg taagtccaat caatattacg gtaagaggac attagattat ggtaccttgc
      241 tctttgttca actgaagcca attcccagcg tttttcaaat attttttctt cttccattat
      301 tgatccctcg caagacgcta cctcaaactt aaataatggg cagcgaaagt ctaattcctt
      361 agatctaagt atagcaaaca aatatgaaaa aaacttagaa ggtagtaaaa gttcttccgt
      421 cttttgcttt gcttctattc ttaatgtgtt tcgttatcgg tgccctacaa agattttcat
      481 taattacaca ctcatttatt gttgtgtatt ttctgacatt tctctcttga tcaattattt
      541 taattccatg ggcatcctga tagttcatag gagaggagcg gctatcagaa atagttgcac
      601 ctatctcttg aacttctttt acaacttctg gaaaatccat ccatcctgct tcagctgtca
      661 aaagtaataa tctataatat ttatctttcc ttttcaatct aagaaatc
//
LOCUS       DAKBVG010000384          699 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.399, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000384 DAKBVG010000000
VERSION     DAKBVG010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..699
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.399"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
BASE COUNT          296 a          110 c          104 g          189 t
ORIGIN      
        1 ataacataat aagaaagtaa agtggattga tttgagatct ttttatactt caaaaaagat
       61 aacacccaac tttcaaaaat atgttttgga agttgggaca aacgagttaa tacattttaa
      121 tacattattt tttattcaaa aaaaatagca caatggcaaa gatcgtaaga gagatcataa
      181 gccccaatga atgactaaaa agaaaagcag aatcacatgc catatcatcc catatttctt
      241 caagaaaata aataagcggg aaaaagggaa ggaatagtag aacatcgatc cagcaccatt
      301 tttttcttct tttgaaataa cataaaacaa taaacatgaa cgtgcaggga agtaaaaaca
      361 aagctttact cataactaac aaaataccag ctccttttat ttttttctcc cccacattca
      421 aagagtaaca atggtatgta cttaataata gaaaaatgga tcaacttgta tgaataacag
      481 ctgaaaagat ctgcaccaaa taaaaaactt ccagccaaaa ctgactggaa gtttattttt
      541 ttctgaaata ttctaacaca aatatgaaaa agatggccga agctaataag ccgatttcgt
      601 agcaaaacaa tgagatacca gagtcgtaat aataaaatcc tctaatctta tggataaaat
      661 agatcatcgg acaagcagat aagagtaagt atttgttag
//
LOCUS       DAKBVG010000385          696 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.400, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000385 DAKBVG010000000
VERSION     DAKBVG010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.400"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            <1..>696
                     /locus_tag="OUD09_002783"
     CDS             <1..>696
                     /locus_tag="OUD09_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HCT9693977.1"
                     /translation="LVATLAVTVGSSVVGCLAVLFISPTAEARALMNVLVTGVVGVAV
                     YISVFRRCKDFKDNEVWNFAVVFCVSLMPHYLSEFILNGSDKIMINYLCGSSDVAIYS
                     VAYSIGALINLVTNSVNSTFAPYQYQKIKSGEYDLLSKRANEVLGLVAAMLFALMLFS
                     KEIVLIFGGQKYIDSVNLIVPICIGIYFNYMFQLFARVQEYFEKKLTVVIPSVLCAIM
                     NIVLNYVFIKLYGY"
BASE COUNT          188 a           93 c          145 g          270 t
ORIGIN      
        1 ttagtagcta cccttgcagt tactgttggc tcatctgtag ttggctgttt agcggttctg
       61 tttatatctc ctaccgctga ggcgagagcc ttaatgaatg tcctagtgac tggtgttgtt
      121 ggcgtggctg tgtatatatc ggtatttaga agatgtaagg attttaagga taatgaagtg
      181 tggaatttcg cagtagtatt ttgtgtatcg ttgatgccac actatttatc tgaatttatt
      241 ctgaatggtt ctgataaaat tatgataaat tacttatgtg gttcgtcaga tgttgctatt
      301 tacagcgtgg catattctat tggtgctcta attaaccttg ttacaaattc tgtcaattcc
      361 acttttgcac cttatcagta tcaaaaaatc aagtcaggag aatatgattt actatcaaaa
      421 agagctaatg aagttcttgg tttggtagcg gctatgcttt ttgcattaat gctatttagt
      481 aaagaaattg tgttgatatt tggtgggcag aaatatatcg acagtgttaa tttaattgta
      541 cctatatgta tcggtattta tttcaactat atgtttcagc tgtttgctag agtacaggaa
      601 tactttgaaa agaaactcac tgtcgttatt ccatcggttt tgtgtgcaat tatgaatatt
      661 gtactaaatt atgtttttat aaagttatat gggtat
//
LOCUS       DAKBVG010000386          692 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.401, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000386 DAKBVG010000000
VERSION     DAKBVG010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.401"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(<1..613)
                     /locus_tag="OUD09_002784"
     CDS             complement(<1..613)
                     /locus_tag="OUD09_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCT9693978.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFL"
BASE COUNT          199 a          137 c          134 g          222 t
ORIGIN      
        1 gcaggaagta ttgagttagt ttgtaaagtc tgcgatcttc aaggatcgta aatagctgat
       61 gggttgtccc atcaacacaa ataaaactca tggaaccaga aacagacttc attgatttga
      121 actcatccat acacaacacc gtcggaagga agttcatacg tggctggtaa tgcttcgttg
      181 cttcacgcaa gatccgtgtc accgtcccat ccgagacgaa gtacttctta gcgattagtt
      241 tacgtgattg attttcgtac agttctatga gaatcgcctg cttcagttcc tttgttagtt
      301 gatgatgttc atcaactaag tccgttttcg cagaaaaggt ctgcccacaa tcatagcata
      361 gaaaacgggt acgttttaat tctaaaatag tcaacttttc tttgactttc aatatctgag
      421 tacgtgtcgt ataggaaccg ttgcggataa tacgaatcga tccacagtgt tcacaatggg
      481 aaggtgagtc atccaatctc cctttaataa actgcgcttg aacgccttta tattcttttt
      541 caatcagcca ttcttcatca aaaaataaat gtttatctgt taatccgagt aattttctag
      601 tatgattatc cattgagaga ctcctttggt aggatgacgt tttggtcgac taaattttac
      661 caaaagtgag tctctttttg agttgaaacg aa
//
LOCUS       DAKBVG010000387          688 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.402, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000387 DAKBVG010000000
VERSION     DAKBVG010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..688
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.402"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            <1..>688
                     /locus_tag="OUD09_002785"
     CDS             <1..>688
                     /locus_tag="OUD09_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693979.1"
                     /translation="ALRKLIYLLAPTYFDNWTKDSIPDEMISAVKGKQKQAIGKYDLE
                     NLLQWIDLYDFEEYLFGENYLFISTGNEKNIVRYKEIIVEELLNLFRETDDLSLSDPF
                     SLWSEVFSKYMYVDLEEIRKDMELIREGRNIVSHNKEIKYSLYKDLIKNLKKYVKQLN
                     EAFKKILIGEIADEKLFDMTNDFEAYIGNHYSNINLPNATKLLTSLSAMTVLNTDSTK
                     KMMETIGPALS"
BASE COUNT          276 a           85 c          122 g          205 t
ORIGIN      
        1 gctttaagaa aattgatata tttattggct ccaacatatt tcgacaactg gacgaaagat
       61 tctattccag acgaaatgat ttctgcagta aaaggaaaac aaaaacaagc aatcggcaag
      121 tatgacttag aaaatctttt acagtggata gatttatacg attttgaaga atacctattt
      181 ggagaaaatt acctatttat ttcaactgga aatgagaaaa atattgtaag gtacaaagag
      241 ataatcgtag aagaattgtt aaatttattt cgagaaactg atgatttaag tctatctgat
      301 cctttttcat tatggagtga agtatttagc aagtatatgt atgttgattt agaagaaatt
      361 cgaaaagaca tggaattgat cagagaagga aggaatattg tgagtcataa taaagagatt
      421 aaatactctt tatataagga tctgataaag aatctaaaga aatacgtgaa acaacttaat
      481 gaagcattta agaaaatact aattggtgaa atagcagatg aaaaactttt cgatatgaca
      541 aatgatttcg aagcatatat agggaatcat tatagtaata taaacctacc caacgctact
      601 aaattattga cgtctttatc agcgatgaca gtattaaata ccgattccac taaaaaaatg
      661 atggagacaa tcggtccggc attatccg
//
LOCUS       DAKBVG010000388          683 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.403, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000388 DAKBVG010000000
VERSION     DAKBVG010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.403"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(133..>683)
                     /locus_tag="OUD09_002786"
     CDS             complement(133..>683)
                     /locus_tag="OUD09_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCT9693980.1"
                     /translation="QNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPGLEMAIDFVRSGD
                     TLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSSTGQLMFHLFAAFA
                     EFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSGTPIKTIAERWNV
                     SRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          221 a          152 c           96 g          214 t
ORIGIN      
        1 taatattaac aaatgaataa aatccttaca acatttgaga ataagttatc agtagacact
       61 tacaaaaaat tctcatacta ttgtcacttt tctagataaa tggcaaaaat tttcagcatt
      121 aaatgacaaa aactaatgag aatttgtctt tccatttttc tcctccattt ttgccaaata
      181 acgataaatg gtggttcttg agacgttcca ccgttccgca atagtcttaa taggtgtacc
      241 gctatccact aaagtcttca gtaattccaa atcttgttca gacaattttt caggacgacc
      301 accaaaccgt cctcttgcac gagcagctgc tcgtcctgct gcggatcgtt ccaggatcaa
      361 attacgctca aattctgcaa atgccgcaaa caaatgaaac atgagctgtc ctgtagaact
      421 tgatttgtcc atcgtaatat tttcttgaat actatgaaaa ctcacaccac gctcattcag
      481 tcgattgacg attgaaatca aatcttccat atttctacct aaacgatcta agcgccaaac
      541 gacaagtgta tcgccagagc gaacaaaatc aatggccatt tctaatcctg gtcgattact
      601 ttttgcccca ctcatatgat cagtaaaaat tttttcacaa ccaaatttat ttaggctgtc
      661 ttcttgtaaa tccaaatttt gta
//
LOCUS       DAKBVG010000389          680 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.404, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000389 DAKBVG010000000
VERSION     DAKBVG010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.404"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
BASE COUNT          241 a          114 c           80 g          245 t
ORIGIN      
        1 ccaaattact tcatcaaaac attgatataa tttttattat atcagtattt attcttgata
       61 cataaatgag aaaataaata ctttaaatct attgactaca agtagtcatt aagtcttcat
      121 aagatgtaca atatgacatt aaaaaaataa aagcttacga ccatccgtta aatctgacac
      181 taaataatgg actgctttct tttgtattag caggttacgt tgactatgaa tattcaccag
      241 attatttgga gaaacctctt caacacgaga acaatgatat taattttaga tttgtctacg
      301 agtcaaaaag taaaacatgg ttagttgatc aattaagcac gataaacaca agcttccatg
      361 aattacctaa acctgaaaat gaagttgaga tggtcattaa aatcttctaa aaaagaagcg
      421 ttgaagggga aatattcctt tcaacgctct tttttatatg cttagaaaca taaataaatt
      481 gtatactttc tttttagccc ctattacttt tcacgaaaaa tatctttttt tcgacgctag
      541 cgtttctcta cttctaatac tgtacacagt ttttgtatta ttgaaaaatt actcattatt
      601 tagcattaat ccacatcgtt ttatcaatcg accttcatac taaactactt taaagtcatc
      661 ttccttctcg tttttcatta
//
LOCUS       DAKBVG010000390          673 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.405, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000390 DAKBVG010000000
VERSION     DAKBVG010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..673
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.405"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            <1..100
                     /locus_tag="OUD09_002787"
     CDS             <1..100
                     /locus_tag="OUD09_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCT9693981.1"
                     /translation="KKNLGSLKAQQKNGAAIYREDEKEYYTKIKLM"
     gene            complement(290..634)
                     /locus_tag="OUD09_002788"
     CDS             complement(290..634)
                     /locus_tag="OUD09_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HCT9693982.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
BASE COUNT          210 a          129 c          105 g          229 t
ORIGIN      
        1 taaaaaaaat cttggcagtc ttaaagcaca acaaaaaaat ggggctgcga tttatcgtga
       61 agatgagaaa gaatactata cgaaaatcaa actgatgtaa aaaagaagga ttccaaaagg
      121 aaagggattt gaatgtcctt tatcaataga acacatttat ttaattaact tataatatag
      181 cagacaaaat tttataaaca aagaaacctt ttatctcttt tcttctagaa aggataaaag
      241 gcttttttgt ttttcctatt tgcttctcat agctagtcag ccaagatttc tatcgtatcc
      301 ccaacgcaat ccgtgcataa cggctcatct tatctgtcgt ccatgctgga taccagacta
      361 gtttgacttc tgctttcgtt acttctggca cttcttttaa tgcgtcatgg atagattctg
      421 ttaaaatatc tgctaatgga cagcccatag ttgttagagt catcttaatg actgtttctc
      481 cattttcagg attgaattct atatcataaa tcaagcctaa attgacgatg tcgattccta
      541 attcaggatc aatcaccatt tccaaggcag caagaatacg ttctttgatt agttctattt
      601 cttgtccaga ccgttcttgg tttgcttcgc tcatcgtacg acactcctta acacgatttt
      661 tatttatcat act
//
LOCUS       DAKBVG010000391          669 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.406, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000391 DAKBVG010000000
VERSION     DAKBVG010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..669
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.406"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(117..572)
                     /locus_tag="OUD09_002789"
                     /pseudo
     CDS             complement(117..572)
                     /locus_tag="OUD09_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
BASE COUNT          205 a          142 c           93 g          229 t
ORIGIN      
        1 aatcatttgt ttaaaatttt cttcatatcg ggtcataaaa attcatcctc tcgtagataa
       61 gtttaattta ttcttgtctc tacgaaaagc tgtctaagat ttgatactaa caccactttc
      121 gtgtctgtat attgtttagc caatacgttt tctactataa cagtctgatt ttcattcact
      181 aattcataaa cagttggatt aacagataaa taagacatca ctgcgatgat accagggaat
      241 acaccatcca ctttattcag cttggctaat tcgatcccta ttagaaaaac agcatagatg
      301 gtcaagaaac ttagtgtgac atagctgatg gcagatgcga taggctttaa ttcttctaaa
      361 aaacgcaagt aacttatctg tgctaagcca ttttccgagt caaagaccat tgctgaaaac
      421 aatgtgccaa aagcacccgt aataattacc ggaatcaatg cagcaaatgc gttttttatc
      481 accatgatat accttaaatt attgattctc gttgccacgt tccctaaaac tttggctaat
      541 ttatccataa aactcattcc agatcccctc attcctgctt cttctccttg acgtcatctc
      601 tttttgtcta ttagtcagcg cttacatttt ggtgaacaga aactatttac tcctcttttt
      661 taccaaata
//
LOCUS       DAKBVG010000392          669 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.407, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000392 DAKBVG010000000
VERSION     DAKBVG010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..669
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.407"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
BASE COUNT          220 a           92 c          121 g          236 t
ORIGIN      
        1 tagcctttta cgtcataagt gttaaaagaa ataattaatt tcaaatatag aaaagggaac
       61 gtatgtttgc atactccttt tttttgagtt ataataatac gcgaagagag ataatttcat
      121 tatttaaaca agctaaaaat cttgatatta cttcttttca ggcaatacga atattattcg
      181 cttacatttt ttgtataaat ttaattgtct gctatgcttg tgatgaattg attttcgttt
      241 tgcacttttg gatgataaat ttggacaatt gaaaatacaa aaaaaagaaa ggcggtatga
      301 actcggaagg ctggtaacct tcgtcagcgt tcatacacgg cattaagatt tggcccttaa
      361 tgcgttaacc tttcagtgtt acggtattac ccgcaatgga tttagtataa cagatattca
      421 aaagtgatac cacttttttt ggctgtgtta aggttaaaaa tacgtgatat tacgatgaat
      481 ctatgcctaa gcataatgtt ataaaaatgt ccgtgtggct ctttgccgta tgacgaacgc
      541 tgaaattaga gggaacgcat gtttggatgc gttccctctt ttttgtttgt catgaacgaa
      601 aatcaattct ctatttttga aaaataacaa gaaaaacttg ttaaaaatat ttaaagaaat
      661 tgggtggtt
//
LOCUS       DAKBVG010000393          660 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.408, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000393 DAKBVG010000000
VERSION     DAKBVG010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.408"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(235..384)
                     /locus_tag="OUD09_002790"
     CDS             complement(235..384)
                     /locus_tag="OUD09_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693983.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
BASE COUNT          218 a          107 c           97 g          238 t
ORIGIN      
        1 atattataca gataataacg gaatattatc atcgaaacag ctaacaaaat tgtcataaaa
       61 aacgagatgg ttgatattag aacttgtgtt tttgtttagg tctagagata aagagagaca
      121 atttccaaat atatgtaatg aatacaaaaa gacgtttgtc gcggattacc gttacaaacg
      181 tcttttattg attttccgag atctcatcag tcgccatttg gaatggttca ctccttatct
      241 agattcgttt aatgctcgga ctgcttgttc gattgtaata ccaaacgcaa cgttattttc
      301 gttttgtgca tcaatcgcca taaaacggtt agaagttgta tcaaaagcta cgcggtattg
      361 catatttgtt tttgtaaacg tcataaaatt catctccttt gtaatgtaat ttagttcttg
      421 aaagtttcct ttaaactttc gaacttatac caagtttagc atcgtacgtt ttttgatgca
      481 attaaaaagc atttatcatt ttccttattt attttttctt aaatggttta gctattttct
      541 tttaatccga taagtagccc aatcatcaag gttacaaata ctattgcttg aatataaatc
      601 atatgctcac ctctttataa ataacttagc aatataaaga aaaaaacgca cgaacaacat
//
LOCUS       DAKBVG010000394          656 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.409, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000394 DAKBVG010000000
VERSION     DAKBVG010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.409"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(89..643)
                     /locus_tag="OUD09_002791"
     CDS             complement(89..643)
                     /locus_tag="OUD09_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCT9693984.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
BASE COUNT          192 a          127 c           73 g          264 t
ORIGIN      
        1 ttttattccg cgaactttat gaaataaaat cgttctagat aaaaatagct tttaatcttt
       61 gtctttactg tttataaaaa tttttaaatt atacacctag tttaaatcca cgaagtgatg
      121 ctcctatttc atcttcagtt ataccaatgt acctttttgt aattgcttgc gagctatgat
      181 taaaaataaa cattaaggta gcaatgtctc tagttttctt gtagtagtga tacccaaaag
      241 tttttcttaa tgtatgtgtt cctatatcat ctcgtcctaa tttttctgct atatctgcat
      301 atatctgata gacacgatcc cttgttatat gtcgaccttg ttgccgactc ggaaataacc
      361 aatcattttc ttctttacct tcagtatata ataaaatttc ttctctcaaa ttatctataa
      421 aaatcggttt tcgcttacct gttttatgtt caacaataat aggcttactt ttgcctcgta
      481 tatctccaac ttttaatcct ataatatctg acatacgtaa cccattattg attccaaaca
      541 aaaataaaaa actatctctt tccggattac ttgttcttct tagccaaaac tgaaaatctt
      601 gtatctcttc atttgtcctt aacggttgta cattatagct cactattgtt tccctt
//
LOCUS       DAKBVG010000395          646 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.410, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000395 DAKBVG010000000
VERSION     DAKBVG010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.410"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(<1..537)
                     /locus_tag="OUD09_002792"
     CDS             complement(<1..537)
                     /locus_tag="OUD09_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin ECF transporter"
                     /protein_id="HCT9693985.1"
                     /translation="MLPTSNSRFPIRRITTLALFSAACIVGRILFTWIPNVQPMTAIL
                     LLLAFYGSYTDALIVTLVSLLGTNLYLGMGSWTISQLIAFSSIVTVFHFLSNCALIKH
                     HISIQAIIAFLCGLLYGLIVSRVEVFIYQLPSFWAYYFQGLFFDILHGTGNLIFYLVL
                     VSVFKRILLPAINSSKNKL"
BASE COUNT          250 a          101 c          118 g          177 t
ORIGIN      
        1 taatttattt ttcgaactat taatagcagg taataaaatt cgtttaaaca cagaaactaa
       61 tacaagataa aagattaaat tacccgttcc atggaggata tcaaaaaata atccttgaaa
      121 ataataggcc caaaaagaag gtagttgata tataaagact tcaactctcg atacgatcaa
      181 tccgtaaaga aggccacata aaaatgcaat gattgcttgg atagagatgt gatgcttaat
      241 cagggcacag ttagataaaa aatgaaaaac cgtaacgata ctactgaagg cgatcagttg
      301 gctgattgtc caactaccca ttcctaaata aaggttcgtg cctaataagc ttactaaagt
      361 aacgattaaa gcatctgtat aacttccata aaaagcgagt aaaagtagaa tggctgtcat
      421 tggttgtaca ttcggtatcc aagtaaaaag gattctacca acaatacagg cggcactaaa
      481 taaagcgagg gtggtaatcc ttctgattgg aaagcgtgaa ttagaggttg gtaacactag
      541 gaaaactccc ttgttttaat tgataatagt tctcattgta tcagaataaa aattaattac
      601 aagattgaat tacactttat atagggctat aatcattggc aggcac
//
LOCUS       DAKBVG010000396          642 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.411, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000396 DAKBVG010000000
VERSION     DAKBVG010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.411"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(<1..544)
                     /locus_tag="OUD09_002793"
     CDS             complement(<1..544)
                     /locus_tag="OUD09_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HCT9693986.1"
                     /translation="MKITEEKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSE
                     ISKLIAYMQKANADVEKELYANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISN
                     IGSFGEDIYKIGMTRRLEPMDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFR
                     KQEVNKINQRKEFFKVSLDEI"
BASE COUNT          181 a          115 c           67 g          279 t
ORIGIN      
        1 ctatttcgtc tagtgaaact ttgaaaaatt cttttctttg attaatttta ttaacttctt
       61 gttttctaaa atgattatgc aatttagttt ccaaagacgg agcgtcctct gaaaaaatca
      121 tcgcatgaac gtcaaattca aatggaaccg atgcactact caattctttt attctatcca
      181 taggttctaa tcttctagtc ataccaattt tatatatgtc ttcaccaaaa gacccaatat
      241 tactaattac atacacataa ccggctctag tatttaattc tctttgtaat acattttcac
      301 gtataacttc taattctttt aatttttctt ccagttcttg aattttattt gcatataatt
      361 ctttttccac atctgcatta gctttttgca tatatgcgat taatttggaa atttcagaat
      421 tgaattgacg ttgttcttta tcaatacgtt tcttttcacg atcaatttct ctacgaactt
      481 tttcctcttc tatcatctgt tcttttattg ctttttgttg ttctttttct tcggtaattt
      541 tcactaaata agagtaatga agttctagtt cttgaagttt aaactctaaa agacccttcc
      601 ttaattcagc attgtcattc ttgaataaat tattaatttt at
//
LOCUS       DAKBVG010000397          638 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.412, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000397 DAKBVG010000000
VERSION     DAKBVG010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..638
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.412"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(19..378)
                     /locus_tag="OUD09_002794"
     CDS             complement(19..378)
                     /locus_tag="OUD09_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HCT9693987.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            complement(537..610)
                     /locus_tag="OUD09_002795"
     tRNA            complement(537..610)
                     /locus_tag="OUD09_002795"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(574..576),aa:Arg,seq:tct)
BASE COUNT          210 a          125 c           94 g          209 t
ORIGIN      
        1 ttcttatttt ttctatattt ataagaaaat agaaagcaat gccaacagag ctagcccacc
       61 ttgtttgagt aaaatccaac gatcgctagt caaactgcca tataatgcaa caaaaaaaat
      121 agatattaat agcatacttg tgatttcttt agatgcggaa gaaagataag ctccataaag
      181 tagtcccact cctaatagtc cattgtacac tccttggttc ttaaacaatg tttgaacaga
      241 ttttctgctt aactcttctt tactcatttt aaaaacccta cttgtcgtgt cagaatcagt
      301 tctcaccgtt tcgatataca tgatataaaa aaattcaatt gcaactagag tcactaaaat
      361 tctagatagt atcgtcatat cttcttcctc ttttctgatg agttttattt ttttgctcct
      421 tataattgta ctcgataagg atgttttttt attttatttg cctttgaaaa caaacaatat
      481 tggcacaaaa aacaaagaaa aaccgttaga aacctcagtt tctaacggtt ttttagcgtc
      541 acaggaggga ttcgaacccc cgaccgtccg cttagaaggc ggatgctcta tccagctgag
      601 ctactatgac aagaatcagc ggaacatcaa taattata
//
LOCUS       DAKBVG010000398          632 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.413, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000398 DAKBVG010000000
VERSION     DAKBVG010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..632
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.413"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            73..234
                     /locus_tag="OUD09_002796"
     CDS             73..234
                     /locus_tag="OUD09_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCT9693988.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
     gene            253..>632
                     /locus_tag="OUD09_002797"
     CDS             253..>632
                     /locus_tag="OUD09_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT9693989.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTK"
BASE COUNT          262 a           62 c          109 g          199 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcagga taaggataag tgtattaatt gtggaaaatg taaaaaagtt
      121 tgccaaatga ataaagattc agttgagaat tgcaatcatc tggagtgtat cagatgtgga
      181 cgatgtaaaa atgcttgtcc agtggatgca attagttatg gagtaagaaa atagagagga
      241 gtagttagga aagtgataaa aaatgctata gcatatatta caagaaagaa aaatagaact
      301 ttaataattt ttatcatatt aactatagtt ctttcttgct tgtattcttg tttaacaata
      361 atgaaatcaa gcaatgaaat agaaaaggct ttatatgaaa gttctaattc ttcaatatca
      421 ataacaaaaa aagatggtaa atattttaat gttaatcaat ttaaagatat tgaaaaatta
      481 aaagaaattg aagaaaaaat aattcaatat gatggattag caaaactaaa agatgctaaa
      541 gtagttagcg gagaacaaag aataaataga gaagatttat ctgacgaatt taagaatgtt
      601 gtttcactcg aagctacaaa taatactaaa ag
//
LOCUS       DAKBVG010000399          622 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.414, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000399 DAKBVG010000000
VERSION     DAKBVG010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..622
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.414"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            7..>622
                     /locus_tag="OUD09_002798"
     CDS             7..>622
                     /locus_tag="OUD09_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693990.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
BASE COUNT          227 a           83 c          118 g          194 t
ORIGIN      
        1 agaaccttgg gttttcaagt gctttttggt tcattaatct taaccatagt gtttttaaag
       61 tggaaaacaa gcaacaaaaa aagtatgtgg agccttgtag gaatagttct tattattttt
      121 actgtgactg cagcaacaga agcaaattat tcgcataaaa ttcagtcgta taaaggtcgt
      181 ttagttatga ccaaaaaaga tgttacacgt tatacatcct caagaactgg aggcctatat
      241 gactatgcac cattagatgc attgaaatat aaagatcatt taaagaaaca tgaagtaaaa
      301 gttggtgatc ggtgggagaa tgcatctcat aaagcatata attccaaaat agtctataca
      361 gtagaagatg ccgatgagca aaatatcaca ttacctgtat tcaattattg gggaacagtt
      421 gtgaaagtaa atgggaaaaa gagcactata gaaaatgaca atggtttagt gtctattaaa
      481 ggtacgggga aaagaactat agttgaggtt tcatctacgt atccaactac gatgattcta
      541 tctttattaa ttagttcaat aacttttata ttcttattta cacgttattt aataagatgc
      601 tgcggtgaaa tgaaaataga tt
//
LOCUS       DAKBVG010000400          618 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.415, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000400 DAKBVG010000000
VERSION     DAKBVG010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..618
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.415"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(135..512)
                     /locus_tag="OUD09_002799"
     CDS             complement(135..512)
                     /locus_tag="OUD09_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693991.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
BASE COUNT          187 a          108 c          109 g          214 t
ORIGIN      
        1 aaccaaaaaa gaagcagatc cgttagcggt tctgcttctt ttagtttatt atattttctt
       61 atcaataata ggaacctgat tttaaaagcg gctacctctt taccgtgcct ctttacatac
      121 aaaaaataga ggaattaagc gtaaatggct tggctattat aactaaaatg ctggttatgg
      181 agatgttgca gcaagttttc gagaaaagcg tattccatct cttgtccatt gaataagatc
      241 tggatacgat aatgattttt gaaacgcttc tgttcatgaa tctgactgat tgaaagttcg
      301 cctttgtgtt ctgtctgata aagaaaagtc cttttacggc attcagcaca gcgcttggtt
      361 gctcggcgga tttttcggaa tgtataaaaa ctagtatctt tattgcataa ataaacgtct
      421 ttgatcgaca gcaaatcatt atatagacag gaacgttctt cagctgttaa ttctgataaa
      481 aaaagctgac cttgttttat tgcatcttcc atcggcaagt gcctcctcga acccattatc
      541 caaattttta gttaattcta atataccatc attaagtata aatgtaaacg cttgttgatg
      601 gtataagatt ttcgtttc
//
LOCUS       DAKBVG010000401          612 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.416, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000401 DAKBVG010000000
VERSION     DAKBVG010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.416"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(196..489)
                     /locus_tag="OUD09_002800"
     CDS             complement(196..489)
                     /locus_tag="OUD09_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein repB"
                     /protein_id="HCT9693992.1"
                     /translation="MDNPEKYQYDNTEIQVFGGFDLESCLALSTGDKRQALKEMLGFI
                     SDNNIMHLKDFADYCMSDRAPAGWFELLTERNTLFIKEYIKSNWQKENQVYKE"
BASE COUNT          198 a          118 c          102 g          194 t
ORIGIN      
        1 cttcgtaaag gtgggctcgc cgaggggtct tgcagacggc tctgtcggct cttttgagcc
       61 gagtatcagc gcacccttat ggtgcaatta gctgatacaa aaaacaaaga gttggctcat
      121 gtaatccaac tcttaaaaca caaaaaaata acccccttgg cttttcagca gggggcatat
      181 tttttattta attcctcact ctttataaac ttggttttct ttttgccaat tcgattttat
      241 gtactctttt ataaaaagag tattcctctc tgttagcaat tcgaaccaac cagcaggagc
      301 tcggtcagac atacaataat cagcaaaatc ttttaaatgc ataatattgt tatccgaaat
      361 aaaaccaagc atttctttta acgcttgtct tttatcacca gtagataacg ctaaacaact
      421 ttcaaggtca aatccaccaa acacttgtat ttctgtatta tcgtattgat acttttcagg
      481 attatccata tgcgttaaat atctaacagg gttctgttgc aaagttttaa atctactatc
      541 aaataaggta gaataataga aaaagatagc aggaggaatg acgatgaatc attttaaagg
      601 aaagcaattt ca
//
LOCUS       DAKBVG010000402          610 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.417, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000402 DAKBVG010000000
VERSION     DAKBVG010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..610
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.417"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(<1..423)
                     /locus_tag="OUD09_002801"
     CDS             complement(<1..423)
                     /locus_tag="OUD09_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCT9693993.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQ"
BASE COUNT          176 a          111 c           87 g          236 t
ORIGIN      
        1 ttgatatttt tttagatttc tttttagtct ttgtaatact ttataagcca cagaataact
       61 cacgtgatgc tgtttagcaa aattttctac tgagatatat ttttcctgaa agaaagccaa
      121 taccattttc atagataaag aatccgttat ataacgagat aacagagctt tgctagaaca
      181 ttttccatta atttccagac atatatccaa ttcactgacg gttactcgca aatttttctc
      241 tagatcgaac ttttgtaaat catgtttcag cttatcgatc gtttttttca agagaaatgt
      301 cgatagcgac aactcttctt gaacattcga tattgactct gataaatttg agcttgcttc
      361 taaatattta agtaatgaat aacttctgtg atcttctttg cttaaaaaaa actcataatt
      421 cattagtcat cagctctttc tacgtatatt actttccatg cattcaccaa ctagctgggg
      481 ataaaactga tttcctggat ccgagtcact gttgtcagcg attcttgttt gtataatata
      541 catcgtcctg tctttttttt aacattttgg agagcgtttt atttggcctt tctcgcgatc
      601 aaacctgtta
//
LOCUS       DAKBVG010000403          605 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.418, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000403 DAKBVG010000000
VERSION     DAKBVG010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..605
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.418"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(137..286)
                     /locus_tag="OUD09_002802"
     CDS             complement(137..286)
                     /locus_tag="OUD09_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCT9693994.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            complement(279..488)
                     /locus_tag="OUD09_002803"
     CDS             complement(279..488)
                     /locus_tag="OUD09_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HCT9693995.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
BASE COUNT          175 a          121 c           58 g          251 t
ORIGIN      
        1 agtttttttg attgtagata accgattttt atatgttctt tcttctactt tcccttgata
       61 ataaggtagg aataatttat aaaaaaactc ctcaaacaac atagtacttt gagaagtttc
      121 tgcttctttt ccataattag aaacaagttc ggtatacgct ttttttcttc tttaactgtt
      181 ttaacccctc tacgtttttt tgtactcttt tccctgttcg tttgtcgaat cccaaactag
      241 cagtaaaatg atatttccca tttttatctt ttttaaaatt aaccatgatt ctgaaaatcc
      301 tcctctacct ctatcttcat acctaaaact tcttctacag attcaacagg tactagtcct
      361 aatcttctat tttcgtaata ggtatagctt tttgaaacca taattgcttt tgcctgtcgt
      421 atgattctct cagactgata cttcccataa cctaatttca ttaattcatt ttttgttata
      481 accaccattt aaccaccaca ttttttattc aaatataaaa aagaacacta ctaaaaatag
      541 tagcgttcct gcatattttt attcaaattt aatcagcttt ataaatcttt gtatacttgc
      601 tcctt
//
LOCUS       DAKBVG010000404          605 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.419, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000404 DAKBVG010000000
VERSION     DAKBVG010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..605
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.419"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(282..>605)
                     /locus_tag="OUD09_002804"
     CDS             complement(282..>605)
                     /locus_tag="OUD09_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9693996.1"
                     /translation="RISYVYTIAKFNLITTEIYLNYLKEVKPILMEMDKEKNELLLNT
                     KFKLTNSDGKSSDVVFYDFDGENYHCFNRTDNSLSMYASIKKEVKKILREEEQYYKEI
                     KNKNQ"
BASE COUNT          190 a           99 c           58 g          258 t
ORIGIN      
        1 tttttaatat caaaactaaa ttcgtatttt tttccgttat tttgatattc taaaaggttg
       61 tcttgtatat atcgtgataa tacttccaaa aaaacttctt taatatcatc cattttgcct
      121 tgtttagggt aatataaata ttcaacaact tgcttgctaa tatgtcctcg ccttcttttc
      181 tttcaacgat ttaaaatttt ccaaataact tgataactac taagtaagcc tcaaacaaca
      241 accacaaatg aatttttcac taatattgat gattgtgata tttactggtt tttatttttt
      301 atttctttat aatattgttc ttcttctctt aatatctttt taacctcctt ttttatgcta
      361 gcatacatcg ataaactatt gtctgttcga ttgaaacaat gataattctc tccatcaaag
      421 tcataaaaaa ccacatcact tgattttcca tctgaattag ttaacttaaa tttagtattg
      481 agtagcaatt catttttttc tttatccatt tccatcaaaa ttggtttaac ttcttttaaa
      541 tagttcaaat atatttcagt tgtaatcaga ttaaactttg caattgtgta cacatatgag
      601 atacg
//
LOCUS       DAKBVG010000405          604 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.420, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000405 DAKBVG010000000
VERSION     DAKBVG010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..604
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.420"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            6..>604
                     /gene="erm"
                     /locus_tag="OUD09_002805"
     CDS             6..>604
                     /gene="erm"
                     /locus_tag="OUD09_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S ribosomal RNA methyltransferase Erm"
                     /protein_id="HCT9693997.1"
                     /translation="MKDSQNFITSKHHINEILRNVHLNTNDNIIEIGSGKGHFTFELA
                     KRCNYVTAIEIDPKLCRITKNKLIEYENFQVINKDILQFKFPKNKSYKIYGNIPYNIS
                     TDIIRKIVFESTATESYLIVEYGFAKRLLNTNRSLALFLMTEVDISILSKIPREYFHP
                     KPRVNSSLIVLKRHPSKISLKDRKQYENFVMKWVNKEYT"
BASE COUNT          260 a           74 c           83 g          187 t
ORIGIN      
        1 aaaatataaa agatagtcaa aactttatta cttcaaagca tcatataaat gaaattttga
       61 gaaatgtaca tttaaataca aatgataata ttattgagat tggttcaggg aaaggtcatt
      121 tcacgtttga attagctaaa aggtgtaatt atgtaaccgc cattgaaata gatcctaaat
      181 tatgtaggat aactaaaaac aaacttattg aatatgagaa cttccaggtt atcaataaag
      241 atattttaca atttaagttt cctaaaaata agtcatataa gatatatgga aatataccct
      301 acaatataag tacagatata attcgaaaaa ttgtttttga aagcacagct acagaaagtt
      361 atttaatagt ggaatatgga tttgctaaaa ggttgctaaa tacaaatcgt tcactagcac
      421 tatttttaat gacagaagtt gatatatcca tattaagtaa aatccctaga gaatactttc
      481 atccaaaacc tagagttaat agctcgttaa ttgtattaaa aagacaccct tcaaaaatat
      541 cactcaaaga tagaaaacaa tatgaaaatt ttgttatgaa atgggttaac aaagaataca
      601 caaa
//
LOCUS       DAKBVG010000406          604 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.421, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000406 DAKBVG010000000
VERSION     DAKBVG010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..604
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.421"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            <1..106
                     /locus_tag="OUD09_002806"
     CDS             <1..106
                     /locus_tag="OUD09_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HCT9693998.1"
                     /translation="MKAIRGLYKKTRKEGALFGFSVCTEIKVLLGIPA"
     gene            161..604
                     /locus_tag="OUD09_002807"
                     /pseudo
     CDS             161..604
                     /locus_tag="OUD09_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611666.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
BASE COUNT          219 a          100 c          106 g          179 t
ORIGIN      
        1 catgaaagcc attcgaggat tatataagaa aacccgaaaa gaaggcgctc tcttcgggtt
       61 ttcggtctgt actgaaatca aggtattatt gggaatccca gcttaaatca tagataccgt
      121 aagagatttt attctttatt taaaactttg caacagaacc gatgaaactt atattaaggt
      181 aaaaggaaag tggcactatt tgtatcgatc cattgattct tcggggataa cattagatat
      241 ttggttacga aaaaagcgtg ataaacaagc agcttatgct ttctttaaac gattggtcaa
      301 acaatttgga gaacctaaag ttcttgtcac tgataaagcc ccttcactaa gtagtgcgtt
      361 taatcaattg cattctgaag gattattttc taagactgaa caccgaacaa ataaatactt
      421 gaataatatc attgaacagg atcatcgatc catcaagaaa aggcacaaat tatatcagtc
      481 cataaggact gattcctcaa caattaaagg aattgaaact atacacgctt tatataaaat
      541 tagccaaaga gacaaaagtc tctttggctt ttcagtgatt catgagatga atacattaat
      601 gagt
//
LOCUS       DAKBVG010000407          597 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.422, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000407 DAKBVG010000000
VERSION     DAKBVG010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.422"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(<1..>597)
                     /locus_tag="OUD09_002808"
     CDS             complement(<1..>597)
                     /locus_tag="OUD09_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HCT9693999.1"
                     /translation="SGTIAEIDYFYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDS
                     LEDKGFYGTYSKDNIRRFLTKKVNYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTG
                     TDKDFDDNLDIFDPIEFDNDERLLLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSY
                     LSDRVFDVLEKLSKSGILKEEYDSSFDEYPSRYILISE"
BASE COUNT          191 a          113 c           67 g          226 t
ORIGIN      
        1 cctctgaaat caaaatatag cgtgatggat actcatcaaa agaagaatcg tattcttcct
       61 ttaaaattcc cgatttactt aatttttcta gtacatcaaa tactctatca ctcaagtaac
      121 tccttaaatt gttatcactt tcccattgaa ctacttttct ataagttgaa ttatcagttg
      181 aaaagataga attttcatcc ataaacgcat atattagcag caatctttca tcattgtcaa
      241 attctattgg atcaaaaata tctaagttgt catcaaaatc tttatcagtt ccggtaactt
      301 ttttgttaat tactgaaggt gtcttgtaag aaatagaatc taaatcaaag cgttttgatt
      361 ctaaattatt ttgtgctttc tcaattaaat aattcacttt ttttgtgaga aaccttctga
      421 tattgtcttt gctatatgtt ccataaaaac ccttatcttc taaagagtcc ctgtacttct
      481 taacttctat atactcttca gccaaaactt gtggaactgg ttcatctaag aaaaaaaccc
      541 ctacctcgcc tttgctattt ttataaaagt aatcaatttc tgctatagtt cccgatc
//
LOCUS       DAKBVG010000408          587 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.423, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000408 DAKBVG010000000
VERSION     DAKBVG010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.423"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(255..554)
                     /locus_tag="OUD09_002809"
     CDS             complement(255..554)
                     /locus_tag="OUD09_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HCT9694000.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          194 a          104 c           66 g          223 t
ORIGIN      
        1 ctatatttcc aatttttaac ttacaaaaat gcaacattaa taaataacga tagtttcata
       61 aaatacactt ttaattaaca ttattagttt atactagctg tttttgtgtt aattaaagga
      121 taaaatacaa tataaagaaa catctgtttc tttatttaga ataaaaaaat acagactctg
      181 ttcaaataaa tgaacctagc ctgcattatg agattgacta tactttttaa ttcattgaga
      241 tgaataactc gtttttataa atgcccaaaa gtaatcggtg ccgaagccat tcctcgacct
      301 aattcttttt caaattttcc tattgttgat atctccatat aaaacggtga tactggttta
      361 gacaattctc ccctgattgc taacggacgt aaactacgtt ccaaattatt tatgacttta
      421 ggataatact gacctttttc taagcctgtc tttgcgtcta tcaaaagtat acgttccttt
      481 tctttgatct ctggatttaa aacgagatca tataatttta gtaccatgtt ttctatttct
      541 tcacttttag acatcacgat ttcctcccaa aaatatattt cttccca
//
LOCUS       DAKBVG010000409          587 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.424, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000409 DAKBVG010000000
VERSION     DAKBVG010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.424"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(239..>587)
                     /locus_tag="OUD09_002810"
     CDS             complement(239..>587)
                     /locus_tag="OUD09_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9694001.1"
                     /translation="KNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLFAGYYFQ
                     RNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVPQILGSL
                     WAVALSYKKARHP"
BASE COUNT          254 a           65 c          120 g          148 t
ORIGIN      
        1 ttagtgtttg gagagaagaa aagaagtaaa aaatagaaaa actagtgata gttttggtga
       61 tagtgatatt aaaaatcaga aaagatagaa aaagcaggag atagataaaa tgctgatatt
      121 tcagcatttt taagagtata aaagagaaaa aaagtaataa atgtatacat gatataagaa
      181 aaacatatat gtataaaaag aagaaagtag cttctctttg tacatatatg gtttgttttt
      241 atggatgccg agctttttta taagataagg ctaccgccca taacgaccca aggatttgtg
      301 gaactaagta aatacctgca agaagcaaga tcagctgggt cggttttaaa ggatgataga
      361 agtaacgcca ttcagtaaaa ttggcgcaaa ataaatagga agtgacagta gatataatat
      421 tggcaatgaa taaagatata atacgcctgt tcctttgaaa ataatatcca gcaaaaagag
      481 ataaaaatat caatgtgatg aacccgctta cggaatgata acgaaaatca agcagtaagg
      541 cgaaatgcac ataaggaaca aaagataaaa gcaacggaag gtttttc
//
LOCUS       DAKBVG010000410          574 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.425, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000410 DAKBVG010000000
VERSION     DAKBVG010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.425"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            114..>574
                     /locus_tag="OUD09_002811"
     CDS             114..>574
                     /locus_tag="OUD09_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCT9694002.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRAL"
BASE COUNT          222 a          102 c           93 g          157 t
ORIGIN      
        1 taagtcgaga gtataaaata ttttgtgtaa atgaaaaaat ccatacaaaa aaggaagtcc
       61 cttctgtaga ataaagttaa cgacaaccaa ttcacagaaa agaggacttc cctatgaatg
      121 attttactac agaaattgtg caaactctag tcactaaagg cgatttaaat gaattattcc
      181 gttcgcactt agaaaaagcg ataaacacac tcctacggac tgaattaacg gcttttttag
      241 attacgaaaa atatgatcgc actggtttta attcaggtaa ttcgagaaac ggttcttact
      301 ttcgatcaat caaaaccgaa tatggtgaat taacattgga aatacctaga gatcgtaatg
      361 gtgagtttaa acaacaaact ttaccagcct acaaaagaac aaacgataca ttggaaacca
      421 ctattatcca tttattcgaa aaaggtgtta cgatgtctga aattgctgat ttgatcgaaa
      481 aaatgtacgg tcatcactat actccacaaa ccatgtccaa catgactaaa gttctgactg
      541 aagaagtaaa tgcctttaaa tccagagcct taaa
//
LOCUS       DAKBVG010000411          530 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.426, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000411 DAKBVG010000000
VERSION     DAKBVG010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.426"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            <1..517
                     /locus_tag="OUD09_002812"
     CDS             <1..517
                     /locus_tag="OUD09_002812"
                     /inference="COORDINATES: protein motif:HMM:NF024997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCT9694003.1"
                     /translation="DFSTETICEKWAADITYIPTKKNGWCYLSSIMDLHTKKIISYTF
                     SKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNKIRHSYSRKG
                     TPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIHSSIHYLTPQ
                     EFEELAKEKMA"
BASE COUNT          202 a           97 c           91 g          140 t
ORIGIN      
        1 ggacttttct actgaaacga tctgtgagaa atgggcagct gacattacgt acattcctac
       61 taagaaaaat ggttggtgtt acttatcttc aatcatggat ttacacacga aaaaaattat
      121 tagttataca ttttcaaaac gaatgactgt ggattgtgtc attcaaacat tgaataaagc
      181 aaaaatacat tatcacattc cagaaggaat gattctacac actgacttgg gcagccaata
      241 cacagcaaga gaagtggaac aatggcttaa aaccaacaaa ataaggcatt cttatagtag
      301 aaaaggaaca ccttatgata atgctggaat cgaatctttc catgcctcat tgaaaaaaga
      361 agaagtctac acgactagct actcagattt tgaagaagca aatcgagcgc tatttagcta
      421 cattgaagga ttttataacc gaaatcgaat ccatagctcg attcactatc taaccccaca
      481 ggaatttgaa gaattagcaa aagaaaaaat ggcttaaccg tctctactaa
//
LOCUS       DAKBVG010000412          526 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.427, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000412 DAKBVG010000000
VERSION     DAKBVG010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.427"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(54..407)
                     /locus_tag="OUD09_002813"
     CDS             complement(54..407)
                     /locus_tag="OUD09_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9694004.1"
                     /translation="MNSFKKKFVSRFLNMKNFFEKKGDYDAYNYVLNLEKQTYYTLRK
                     NDDNDFWTMLPKMLSIDSKLSLLEEFINLDIEEFCGGKELINMVESDYHTYYKESCGY
                     KVDEQCPQSIIFYIE"
BASE COUNT          174 a           90 c           49 g          213 t
ORIGIN      
        1 ttgtaaatac taaatcagta gcttctagtt gctttcctgc ttaatagttc atattactca
       61 atgtaaaaaa taattgattg tggacattgc tcatccactt tgtatccaca actttcttta
      121 taataggtat gataatcact ctctaccata ttaattaact cttttccccc gcaaaattct
      181 tcaatgtcta aattaataaa ttcctctaaa agggaaagct tagagtcaat cgaaagcatt
      241 ttaggtaaca tagtccaaaa gtcattatca tcgtttttac gtagtgtata atatgtttgc
      301 ttttctagat ttaatacata attatatgca tcataatctc cctttttttc aaaaaaattt
      361 ttcatattta aaaatctact cacaaatttt ttcttgaaac tattcatttt ctcacctcta
      421 tttattcttt ttcttgtcca tagcataact tttgaataga gtaactaaat tcaaaagcta
      481 ttatagtata aaaatacact ttttgttgtt caaatttttc atctaa
//
LOCUS       DAKBVG010000413          525 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.428, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000413 DAKBVG010000000
VERSION     DAKBVG010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.428"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            <1..>525
                     /locus_tag="OUD09_002814"
     CDS             <1..>525
                     /locus_tag="OUD09_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCT9694005.1"
                     /translation="SARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIH
                     EREKDYSQFSNEFGHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKL
                     NQWFESVPKNLFKSITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLL
                     RRDGLLKSMDFNSV"
BASE COUNT          178 a           89 c          104 g          154 t
ORIGIN      
        1 gttctgctcg taccatttat cgtatgttca aaaaggggct atttgattct tctgacttac
       61 cgatgaaagg caagcgtaaa ccgaatggac atcaagaaag acgtggaaaa caaactttcc
      121 gccgctctat tcatgaacgt gaaaaggatt atagccaatt ctcaaatgag tttggtcacc
      181 ttgaaggtga cactatcgta ggtctgaaac ataaaagtgc tgtaattacc ttagttgaac
      241 gattatcaaa agttatcatc acattgaaac cgtgtggtag acaagcgatt gatattgaaa
      301 aaaaattaaa tcaatggttt gaatctgtac cgaaaaacct attcaaatcc atcacttttg
      361 attgtggaaa ggaattttca aattggaaac agatcagtaa tgtcaatgat attgccattt
      421 atttcgctga tccaggaacg ccgtctcaaa gaggcctaaa cgagaattct aacggattgt
      481 tacgtagaga tggtttattg aaatctatgg atttcaattc agtag
//
LOCUS       DAKBVG010000414          511 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.429, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000414 DAKBVG010000000
VERSION     DAKBVG010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.429"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
BASE COUNT          170 a           94 c           51 g          196 t
ORIGIN      
        1 tctactttca cgtaaaaatt ctgaactaaa taaaatctat tgataatatt tttttcttcc
       61 actttttgta tatcctacag gtatagtgga tttaattatc cccaatgcct ctgaagtaac
      121 agacatcact aattcaataa cacattcact accatagttt gtaggaacaa aaatgacaac
      181 aaaatctgct tccttatgtg atgtttttcc atctgtccaa attttattac caatgaaatc
      241 tagtttaaaa tccctcttag aaactagttc actctatatt tttattcagt ttcattagtt
      301 ctgttttttt gtcttcagtc accatataca tatatatctt aattatctat ccatttcatc
      361 ctacgcattc ttgcgtaaat acaattcact ctgttgagct gatataaatc attggtggta
      421 caaagataaa atgataactg tttctatacc tccaaaaatt ccagtaaata tatctataga
      481 tagtttcaaa aaaaattgtc ttagtttcat a
//
LOCUS       DAKBVG010000415          511 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.430, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000415 DAKBVG010000000
VERSION     DAKBVG010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.430"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            17..>511
                     /locus_tag="OUD09_002815"
     CDS             17..>511
                     /locus_tag="OUD09_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCT9694006.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFI"
BASE COUNT          134 a           83 c           95 g          199 t
ORIGIN      
        1 ttaaggaagt gaataaatga aagaaacgag acaatttata tggacataca ttaagattat
       61 ttttgccctc ttcatattag gagtcgctat caatatgttt ttagggcctc atcatattgc
      121 tgctggaggt gtcagcggtc taggaatatt actggaagct gctttaggct ttgaccgagc
      181 tatagttatt ttagtgttga atatcgttat gttggtacta gctcttctat ttttaggtaa
      241 aaaacccttt tttaaagtac ttttcggaag cttggttttt cctttaatca ttgcaatagt
      301 tccagaaaca atgattactt cagatcggtt gttgtctgtc atttttggaa gtgcgatttt
      361 tgctttagga gttgcgattt tatataaaaa caactcttct agcggcggta cgacgatccc
      421 tcccctcatt tttaaaaaat atttccactt gaatacttcg attggcttat tttttacaga
      481 cgcattcgtt gtttctctta atctgtttat t
//
LOCUS       DAKBVG010000416          508 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.431, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000416 DAKBVG010000000
VERSION     DAKBVG010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.431"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            45..>508
                     /locus_tag="OUD09_002816"
     CDS             45..>508
                     /locus_tag="OUD09_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HCT9694007.1"
                     /translation="MFNLDKIITWRENWSKNILGELEVGRVYTNKEISEKFKCFKQGG
                     MRRSHQTNTLVLFSDQTGSNVYKDKWLNGILQYTGMGLKGDQVLDKNQNKVLANSKSN
                     FVKIHLFETFKPKEHTYLGEVYLAGQIYTVNEKDSSGNSRKVYKFPLALINQ"
BASE COUNT          208 a           51 c           99 g          150 t
ORIGIN      
        1 tattagctga tttaattaga aatggcatgc caaaagaggg agaaatgttt aatcttgata
       61 agattattac atggcgagaa aattggtcta aaaacatatt aggtgagtta gaagttggta
      121 gagtatatac taacaaagaa atttcagaga agtttaaatg tttcaaacag ggtggaatga
      181 gaagatctca tcaaactaat actcttgtac tgtttagtga tcaaacagga agtaatgttt
      241 ataaagataa gtggttaaat ggtattttac aatataccgg tatggggcta aaaggggatc
      301 aagtattgga taagaatcaa aataaagttt tagccaattc aaaaagtaat tttgtaaaga
      361 ttcatttatt tgaaacattt aaaccaaaag aacacacgta tctgggagaa gtatatttag
      421 caggacaaat atatactgtt aatgaaaaag atagttcggg caacagcaga aaagtatata
      481 aatttccatt agctttaata aatcaaga
//
LOCUS       DAKBVG010000417          501 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.432, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000417 DAKBVG010000000
VERSION     DAKBVG010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.432"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            44..325
                     /locus_tag="OUD09_002817"
                     /pseudo
     CDS             44..325
                     /locus_tag="OUD09_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
     gene            399..>501
                     /locus_tag="OUD09_002818"
     CDS             399..>501
                     /locus_tag="OUD09_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HCT9694008.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQEL"
BASE COUNT          187 a           62 c           90 g          162 t
ORIGIN      
        1 cttataattt tttgttaatg aacaagatag gaagaaacat ttaatggaca gttttcagag
       61 attgtcacta gtaaatcaat ttgagttatt ggctaaattt gaggaccaaa acagatctta
      121 ttatgaaaga aaaatcgaaa tattgagaga aggttacgag tatcattatg atgatgaaat
      181 ttggagtgat ctttctgagc catttccgaa agaagattct cgatttgtat tagatgtatt
      241 gaacatgtat cgagatatca attttagtaa atcaaagcta aacttagatg aaaaccaaat
      301 tactaattca tactatactc atttcggttc tgttgcaaag ttttccaaaa aatctatttt
      361 agtgtaaaat tgagaaaaaa gacagagagg acagagtaat gaatcatttt aaaggcaaac
      421 aattcaaaaa agacgtcatt attgtcgctg ttggttacta cctgcgttac aatctaagct
      481 atcgtgaagt tcaggaattg t
//
LOCUS       DAKBVG010000418          500 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.433, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000418 DAKBVG010000000
VERSION     DAKBVG010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.433"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            48..311
                     /locus_tag="OUD09_002819"
     CDS             48..311
                     /locus_tag="OUD09_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
                     /protein_id="HCT9694009.1"
                     /translation="MNKNTSLHEESLIIIEALGGKENIESVEACATRLRVAVTNGEII
                     QKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            324..>500
                     /locus_tag="OUD09_002820"
     CDS             324..>500
                     /locus_tag="OUD09_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCT9694010.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVT"
BASE COUNT          177 a           73 c          100 g          150 t
ORIGIN      
        1 ctattcctgg aatggaagaa gaatcagaag ttcattctga aaaagtaata aacaaaaata
       61 catcattaca tgaagagtct cttatcatta ttgaagctct tggtggaaaa gaaaatattg
      121 agtctgttga agcttgtgca actcgtttgc gtgttgctgt tacgaatggg gagattattc
      181 aaaaacaaac aataaaagac actggtgcaa ctgctgtctt cgaagtgaag ggtggcatcc
      241 aagctgtttt tggagggaaa gctgacttat taagtcaaga aatcaatcaa atattaggaa
      301 aagataatta ggaggttttt aatatgtttg aaattttcaa aaagaaaaaa caagaaactt
      361 tttatgcacc ttgttccgga aaaatcattt ccatcacaga agtaaatgat cctgtctttt
      421 cacaaaaatt actaggtgat ggttttgccg tcatacctga tggttcaaaa atttatgctc
      481 cattggctgg tcgagtaaca
//
LOCUS       DAKBVG010000419          500 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.434, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000419 DAKBVG010000000
VERSION     DAKBVG010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.434"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            55..>500
                     /locus_tag="OUD09_002821"
     CDS             55..>500
                     /locus_tag="OUD09_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCT9694011.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYR"
BASE COUNT          164 a           78 c          103 g          155 t
ORIGIN      
        1 tgcataaaat tcacttgaat acaaaaacta atatgttagt ttaggagggg aaaaatgatt
       61 gatttaaaca actggaggaa ctttatccct gaattatctc aaaaagaagt agaaatacct
      121 cattttgacc gtgcatcatt aagagaaaat ggacaaaaaa atcctgtctg gatccatttc
      181 ggtggcggaa atctttatcg aggatttcat gcagaaatcg ctcaacgttt agcggatcta
      241 ggcgaattgt ccactggtgt cattgtttgt gaaacattcg atgaagaaat aattgaaagg
      301 gcttaccaag attttgatga tttgattctt gaagtggtca tgccaagcag tggagagttc
      361 gtcaaacgta tattagctgc gacagctgac agtctgtatt gtcatccaga ttgttctgaa
      421 cactttgata agatgattcg acactttgaa aatcctagtt tacaattagt tacttttgct
      481 attactgaaa aaggttatcg
//
LOCUS       DAKBVG010000420          499 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.435, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000420 DAKBVG010000000
VERSION     DAKBVG010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.435"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
BASE COUNT          171 a           67 c          100 g          161 t
ORIGIN      
        1 acggtatgtg gttaaaaaag aaaaatagct aggagatttt tgagagatga ctggtaaatt
       61 taaaaatgat agaaatggag agtatgtatg agtaataaac aatttttatg gtcttctttt
      121 gcatttattg ttgttgatat ttttttccga tattttaata acgatatgat atttaactgg
      181 caaagaatta taatcgatta ctgtattttt tgtataatat attttttagt gaaaacattt
      241 ctaaaatacg caaaaaaata aaattgaaga tgttattaaa tcgcgattat ttacggagat
      301 ttatataaat aaaaaccccc tgacgaagtc gaagggggtt tttattttgg tttgaggttt
      361 gcatacctac cgaaaaggta gggcagcaaa tcgtcaaaca ggtatcagct aattgcacca
      421 taagggtgcg ctgatactcg gctcaaaaga gccgaaagag ccgtctgcaa gacccctcgg
      481 cgagcccaca ctttgcttg
//
LOCUS       DAKBVG010000421          499 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.436, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000421 DAKBVG010000000
VERSION     DAKBVG010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.436"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(<1..>499)
                     /locus_tag="OUD09_002822"
     CDS             complement(<1..>499)
                     /locus_tag="OUD09_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCT9694012.1"
                     /translation="KLDDSPSYCEHCRSVRIIRNGSYTTRTQILKVKEKLTILELKRT
                     RFLCHDCGRTFSAKTDLVDEHHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTR
                     ILREATKHYQSRMNFLPTVLCMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYK
                     LTQYFL"
BASE COUNT          144 a          101 c          105 g          149 t
ORIGIN      
        1 gcaggaagta ttgagttagt ttgtaaagtc tgcgatcttc aaggatcgta aatagctgat
       61 gggttgtccc atcaacacaa ataaaactca tggaaccaga aacagacttc attgatttga
      121 actcatccat acacaacacc gtcggaagga agttcatacg tgactggtaa tgcttcgttg
      181 cttcacgcaa gatccgtgtc accgtcccat ccgagacgaa gtacttctta gcgattagtt
      241 tacgtgattg attttcgtac agttccatga gaattgcctg cttcagttcc tttgttagtt
      301 gatgatgttc atcaactaag tccgttttcg cagaaaaggt ccttccacaa tcatggcaga
      361 gaaaacgggt acgttttaat tctaaaatag ttaacttttc tttgactttc aatatctgag
      421 tgcgtgtcgt ataggaaccg ttgcggataa tacgaaccga tcgacagtgt tcacaatagg
      481 aaggagagtc atccaattt
//
LOCUS       DAKBVG010000422          491 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.437, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000422 DAKBVG010000000
VERSION     DAKBVG010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.437"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            <1..>491
                     /locus_tag="OUD09_002823"
     CDS             <1..>491
                     /locus_tag="OUD09_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HCT9694013.1"
                     /translation="VGYYLRYNLSYREVQEILYDRGINVSHTTIYRWVQEYGKLLYQI
                     WKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWLRKKRDTQAAYAFLKR
                     LVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLNNLIEQDHRPVKRRN
                     KFYR"
BASE COUNT          174 a           84 c           94 g          139 t
ORIGIN      
        1 gtgggctact atcttcgtta taaccttagc tatcgtgaag ttcaagaaat cttatatgat
       61 cgtggcatta acgtttctca tacgacgatt tatcgttggg tgcaagaata tggcaaacta
      121 ctctatcaaa tttggaaaaa gaaaaataaa aaatcctttt attcatggaa aatggatgaa
      181 acgtacatca aaattaaagg aaaatggcat tatttgtatc gagccatcga tgcagatggt
      241 ttaaccttgg atatttggtt acgtaaaaaa cgggacacac aagcagccta tgcttttctt
      301 aagcggttag tgaagcagtt tgatgaaccg aaggttgtag tcacagataa agccccctct
      361 attacaagtg cctttaagaa actaaaagaa tacggctttt atcaagggac agaacatcgt
      421 accattaaat acctgaataa tttgattgaa caagaccatc gtccagtaaa gagacgcaat
      481 aaattctatc g
//
LOCUS       DAKBVG010000423          489 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.438, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000423 DAKBVG010000000
VERSION     DAKBVG010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.438"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            <1..>489
                     /locus_tag="OUD09_002824"
     CDS             <1..>489
                     /locus_tag="OUD09_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCT9694014.1"
                     /translation="TRYQLIKQLIRTKQHFLENLTYKCNTLAREMRDESTSLFSATLI
                     SLMTEDFTLDELAELPLEAFCDLLQEKGKGRFKQPEKIAKAIQRAITMSYRLGALAQE
                     SINVVLSVLVREIRALEKNIKELDKAIEQVIVVIPEYQCLTSIPGVGKVYAAGLIAEI
                     GQ"
BASE COUNT          165 a           94 c           90 g          140 t
ORIGIN      
        1 gtactcgcta tcaacttatt aagcaattga ttcgtacaaa gcaacatttt cttgaaaatc
       61 taacgtacaa atgtaatacg cttgctcgag aaatgcgaga cgaatcgacg agtcttttta
      121 gtgcaaccct catttcactc atgacggaag atttcacatt ggatgaactt gctgagcttc
      181 ctttagaagc tttctgtgat ttacttcaag aaaaaggaaa aggccgtttc aagcaaccag
      241 aaaaaatcgc taaagcaatt caacgtgcaa ttaccatgag ttaccgccta ggtgctcttg
      301 cacaagaatc aattaatgtt gttttaagtg ttctcgtacg agaaatacga gcacttgaaa
      361 aaaatatcaa agaattagat aaagccatcg aacaagtaat tgttgtcatt ccagaatatc
      421 aatgcttaac cagtattcca ggcgttggta aagtctatgc tgccggttta atcgctgaaa
      481 ttggtcaaa
//
LOCUS       DAKBVG010000424          468 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.439, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000424 DAKBVG010000000
VERSION     DAKBVG010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.439"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            <1..>468
                     /locus_tag="OUD09_002825"
     CDS             <1..>468
                     /locus_tag="OUD09_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HCT9694015.1"
                     /translation="CVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLA
                     YTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYF
                     YGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHK"
BASE COUNT          155 a           86 c           83 g          144 t
ORIGIN      
        1 cttgtgttat atggggcata attaatggct atactagcca aagagccacg tatagagcgg
       61 tttgttccgt cttatttcct aatggtgact ttcctagtag gagtcgattc actcgcttaa
      121 gttcaaactt agcttacact atcaagatta ttcgatactt tttcatcaag aagctcacca
      181 aaggtgaact agtcggaatt atagatagtt ttcctagtcc attatgtaaa ccagttagaa
      241 atagacaagc aaaactattg aatcaaatag ccaaagttgg ctataactca acaaaaaaat
      301 cttattttta tggtcttaaa attcatatga tcgttactaa gactggcttt ccgattactt
      361 actcaatcac gaatccaggt gtccatgacg tgaaagtgtt agagacttta tcggaagaag
      421 cgaatcttcc caatatccta ggggacaaag gatatattag ccataaaa
//
LOCUS       DAKBVG010000425          467 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.440, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000425 DAKBVG010000000
VERSION     DAKBVG010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.440"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(1..438)
                     /locus_tag="OUD09_002826"
                     /pseudo
     CDS             complement(1..438)
                     /locus_tag="OUD09_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          110 a           88 c           80 g          189 t
ORIGIN      
        1 ttcggctgcg gataaattct tcagcgacta cttcttcaag ttctgattcg tctttctttg
       61 gttttgattg ataataatag gactgacgtg atagacctaa tattctgcac atcgctgata
      121 tagggtaaag atgcttattc gcatcgatta cttgtctctt cgtccgaata tcagcgctgc
      181 ttgctttaaa atatcatttt ccatttctaa ttgctggttt cttttacgta gttctaacaa
      241 ttctttttgt tcaggcgtaa gattatcttt ttctttgaat gaaccactcg ttttagattg
      301 ctttacccat ttgtcaaatg ctgaagccgt tagttcatat tctcgaatga tttctacacg
      361 tggctttcca gctaagtaaa gattgacgat ttgttgcttg aattcttgtg aaaaagttct
      421 tcgtgttctc ttagacataa aaattcctcc tggtatgttt tcttcta
//
LOCUS       DAKBVG010000426          466 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.441, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000426 DAKBVG010000000
VERSION     DAKBVG010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.441"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(73..144)
                     /locus_tag="OUD09_002827"
     tRNA            complement(73..144)
                     /locus_tag="OUD09_002827"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(109..111),aa:Glu,seq:ttc)
     gene            complement(156..229)
                     /locus_tag="OUD09_002828"
     tRNA            complement(156..229)
                     /locus_tag="OUD09_002828"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(193..195),aa:Ile,seq:gat)
     gene            complement(242..312)
                     /locus_tag="OUD09_002829"
     tRNA            complement(242..312)
                     /locus_tag="OUD09_002829"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(278..280),aa:Gly,seq:tcc)
     gene            complement(317..389)
                     /locus_tag="OUD09_002830"
     tRNA            complement(317..389)
                     /locus_tag="OUD09_002830"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(354..356),aa:Phe,seq:gaa)
     gene            complement(393..465)
                     /locus_tag="OUD09_002831"
     tRNA            complement(393..465)
                     /locus_tag="OUD09_002831"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(430..432),aa:Asp,seq:gtc)
BASE COUNT          143 a          127 c          103 g           93 t
ORIGIN      
        1 gataactctg ctaagctttt taaaatcatt ctttcagata aaaagaaaaa gatactcaaa
       61 aagtatcttc catgacccgt acgggattcg aacccgtgtt accgccgtga aagggcggtg
      121 tcttaaccgc ttgaccaacg ggccaataaa ataaatgggc ctaaatggac tcgaaccatc
      181 gacctcacgc ttatcaggcg tgcgctctaa ccagctgagc tataggccca taaaaactaa
      241 aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt tttaccacta
      301 aactacaccc gcataatggc gcgggacaga atcgaactgc cgacacatgg agcttcaatc
      361 cattgctcta ccaactgagc taccgagcca aacggttccg acgggaatcg aacccgcgat
      421 ctcctgcgtg acaggcaggc atgttaaccc ctacaccacg gaacca
//
LOCUS       DAKBVG010000427          450 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.442, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000427 DAKBVG010000000
VERSION     DAKBVG010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.442"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            43..204
                     /locus_tag="OUD09_002832"
     CDS             43..204
                     /locus_tag="OUD09_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCT9694016.1"
                     /translation="MKIAYIRVSSIDQNEQRQLEEMKKFGAEKIFIEKQSGATITHRP
                     IFQEALEFV"
     gene            223..372
                     /locus_tag="OUD09_002833"
     CDS             223..372
                     /locus_tag="OUD09_002833"
                     /inference="COORDINATES: protein motif:HMM:NF012464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HCT9694017.1"
                     /translation="MEAIDRLGRNYDEIIESVNYLKRKNVQLIITSLPIMAEAILSWI
                     SSSRI"
BASE COUNT          163 a           70 c           90 g          127 t
ORIGIN      
        1 ttagggtgtg ttttaaatag ttcgagaaag tagggtaaat ttttgaaaat tgcttatatt
       61 cgagtttcgt cgattgatca aaatgaacaa aggcaacttg aagaaatgaa gaagttcggc
      121 gcagaaaaaa tatttattga gaaacaatct ggagcaacga ttactcatcg accaattttc
      181 caagaagctt tagaatttgt ctgagaacaa gatattttta tcgtggaagc catcgatcga
      241 cttggaagaa attatgacga gatcattgaa tcggttaatt atctaaaaag aaaaaatgta
      301 caacttatca tcactagttt accaatcatg gctgaagcaa tcctctcttg gataagttca
      361 tcaaggattt aatcattcag atcctggcca tgattgccga acaggaacgt acagaatcta
      421 aacgacgcca ggcacaagga attaagattg
//
LOCUS       DAKBVG010000428          445 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.443, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000428 DAKBVG010000000
VERSION     DAKBVG010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.443"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            10..351
                     /locus_tag="OUD09_002834"
     CDS             10..351
                     /locus_tag="OUD09_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HCT9694018.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT          161 a           47 c          103 g          134 t
ORIGIN      
        1 gaatgacaaa tgaaaaatta tgatccgaat atacgattgg gcacacatac tattaaggtt
       61 tctttccaac gttgggatta caaaggattt gtgacgttta gaaggggcgg aaattgtaaa
      121 ggattagatg ttttagcatt agacgaggat gatttatacg atcaaacatt gactgataat
      181 ccaattggat tcggcttgct acctgaagat gatgaaggta atgaatggtt taaaatgacg
      241 cttatgaatg ataatggaga cgaattatct gttgaagata cttggtcata tttgagtgat
      301 tatattgtca gctttgaaat cattgaattt gtagcggata aagaagaata aaaactggag
      361 gaataaattt tacagataaa ggcttagaag gagtcacgac gattaataaa aaggtagtat
      421 taccttaggc attgagtaag gaatt
//
LOCUS       DAKBVG010000429          424 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.444, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000429 DAKBVG010000000
VERSION     DAKBVG010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.444"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            45..239
                     /locus_tag="OUD09_002835"
     CDS             45..239
                     /locus_tag="OUD09_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9694019.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
BASE COUNT          184 a           55 c           79 g          106 t
ORIGIN      
        1 atatttgaat attaactaaa aacagcaaga aagaagagga tcaaatgtat tatgtagaag
       61 taaaaacaaa aggcgtcaaa aacaaacaat atgtgaaagg tatcagtaac gaatatcctt
      121 tacttggttc atggaaagaa gctgctcctt tttccaaacc atgtgccata aaaataaaga
      181 acgagctaga aaaagaactg acttgtggca aagctgttgt agatatcatc gaaaagtaaa
      241 tccaatgtat agaaaggaag atcacacatg aaaagcgacg tacaaattgc taagattata
      301 gaattagaag aagaagacgc agtgttgaca attgaaaaaa aataaatgtt tgtataccat
      361 atgattattt caatatgtat cctggaattg gagatctcgt agagctatat caagatgaat
      421 caaa
//
LOCUS       DAKBVG010000430          419 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.445, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000430 DAKBVG010000000
VERSION     DAKBVG010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.445"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
BASE COUNT          144 a           58 c           60 g          157 t
ORIGIN      
        1 agatataata caaaaaggca aagatatttt caaaacattt tttcttgatg tgatatcctt
       61 gtcttttttg taatgttttt aacttattgc ttttgcaaaa tttatttacc tgttttctgg
      121 atcaacgata gtgtatatta tgctatcctt gtttggattt taggacgttt aaagaggcac
      181 ttttttacat aacttaatcc atagaccttt taagcatgct gtgagataat aataaagaag
      241 agtttaaagc gcaccccaaa ccacttcccc ataagtgtat tacgctttaa actcttttat
      301 atttgaattc actaagaagc ataccatata tttgaattca ctaagaagca taccatattt
      361 ttgaaaaaaa gtgagaaaaa aggcttataa ttaggattta gagtaattaa ttagatgtt
//
LOCUS       DAKBVG010000431          402 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.446, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000431 DAKBVG010000000
VERSION     DAKBVG010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.446"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            <1..220
                     /locus_tag="OUD09_002836"
     CDS             <1..220
                     /locus_tag="OUD09_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCT9694020.1"
                     /translation="FGHSYVFAFYDYRTNITEVDLVISNYYIDTGKTPLLLMKNIPTE
                     RNWRLFEKTIYQLKKEKEVVKSAFCPEY"
BASE COUNT          139 a           57 c           79 g          127 t
ORIGIN      
        1 ttttgggcat tcttatgttt ttgcttttta tgattacagg acaaatataa ctgaagtaga
       61 tttggttatt tctaattact acatcgacac tggaaaaacg ccattactgt taatgaaaaa
      121 tatacctacc gaaagaaact ggcgtttgtt cgagaaaacg atttatcaat tgaaaaaaga
      181 aaaagaggtt gtgaaatcag cgttttgtcc tgagtattag agaaacaaac ggaaaaatag
      241 tttttcatat tttgccgttt ttgagctaat accgagggac agctgattga acaccattta
      301 ttgagagatt gtgaaaaaac tgtcctgcat caagtaataa gaacagccaa gtaaaaacag
      361 cttctcatgt ttgttgaata ttgtgacttg tgaccgagga gt
//
LOCUS       DAKBVG010000432          378 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.447, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000432 DAKBVG010000000
VERSION     DAKBVG010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.447"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
BASE COUNT           98 a           96 c           76 g          108 t
ORIGIN      
        1 attttgactt tcaaaatcac ttttgctacc agaagttgag gcgaagtgtt ttaagcatag
       61 ttgtacccta gtttgaccgt taaaaataaa taacgatcgc tagagtgcaa ctatgtttgt
      121 ttgccctacc gtcagcgtcg gcttggcctc tcacatccat taccgtcggg attatctccg
      181 ttacataccc ttacaaaata cgccctgtaa gcattgcatg aaaccaccgt gcatttgcac
      241 cgtatttccg ccctagccgt tcaaatcggt cgctaccacc attcagaaac acgtgcggta
      301 atggaaggtt tggaggaagc tatccagacc actaacgatt gtccctatcg tcgtacgttt
      361 cactcagatc aaggttgg
//
LOCUS       DAKBVG010000433          371 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.448, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000433 DAKBVG010000000
VERSION     DAKBVG010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.448"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(284..>371)
                     /locus_tag="OUD09_002837"
     CDS             complement(284..>371)
                     /locus_tag="OUD09_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCT9694021.1"
                     /translation="FKERKLKKIENLPLQDMAKIDQIIQYMF"
BASE COUNT          119 a           57 c           53 g          142 t
ORIGIN      
        1 gtctagttaa ttgtagccta tccattcttt ctgttaaagt attgtcgcga cttttcgtat
       61 acgttaagat atgtcgtgaa aaaacaagtt ttgtgacaca aaaaaacgaa tgacttgagg
      121 aaatcacctc aatcattcgt ttaaaatgct atatattctt tagtttaaga tacggaaatt
      181 aatcccatcg accacactag tagaatgaac gacatttact tggccttttt ttaatttcgt
      241 tatgtttagc atataaaaat attatagagt ttacttacaa tgcttaaaac atatactgta
      301 taatctgatc aattttagcc atgtcttgca atggtaagtt ttctattttt ttgagttttc
      361 gttccttaaa a
//
LOCUS       DAKBVG010000434          368 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.449, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000434 DAKBVG010000000
VERSION     DAKBVG010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.449"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            <1..342
                     /locus_tag="OUD09_002838"
     CDS             <1..342
                     /locus_tag="OUD09_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021424053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HCT9694022.1"
                     /translation="KKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLL
                     EDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENG
                     RKEIYKRTYKD"
BASE COUNT          159 a           19 c           65 g          125 t
ORIGIN      
        1 aaaaagtgtt tatgccataa tgattttagt tgtaatcatc tattgttaga tggcaataat
       61 agattaactg gaataattga ttttggagat tctggaatta tagatgaata ttgtgatttt
      121 atatacttac ttgaagatag tgaagaagaa ataggaacaa attttggaga agatatatta
      181 agaatgtatg gaaatataga tattgagaaa gcaaaagaat atcaagatat agttgaagaa
      241 tattatccta ttgaaactat tgtttatgga attaaaaata ttaaacagga atttatcgaa
      301 aatggtagaa aagaaattta taaaaggact tataaagatt gattatataa tatatgaaaa
      361 gctattat
//
LOCUS       DAKBVG010000435          366 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.450, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000435 DAKBVG010000000
VERSION     DAKBVG010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.450"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(<1..188)
                     /locus_tag="OUD09_002839"
     CDS             complement(<1..188)
                     /locus_tag="OUD09_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9694023.1"
                     /translation="MVHSTIGVVVEKSRDNLVFVTEIQTGRSFVVTDKAAKAYQSSDI
                     LTLNLTTKTFVDATEDYP"
BASE COUNT          104 a           81 c           58 g          123 t
ORIGIN      
        1 aatggataat cttctgtcgc atcgacgaac gtcttcgtag tcagatttaa cgttaaaata
       61 tcgctacttt gataagcttt tgccgctttg tctgtgacga caaaagatct gcctgtttga
      121 atttctgtca caaaaacgag attgtctctg cttttttcaa caacaactcc aatcgttgaa
      181 tgaaccattt ctgcttcctc ccttctcttt cttttgatat aatgtagtcg caaatcgcac
      241 cactcaatga gttggcggaa aacaattcat tgaaaaaaag agtcctggac aatcacgttc
      301 aggactcttt tacttctttt attcacttaa caactaacac tctgtgagtg aaatggactc
      361 ttcaat
//
LOCUS       DAKBVG010000436          366 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.451, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000436 DAKBVG010000000
VERSION     DAKBVG010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.451"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            123..>366
                     /locus_tag="OUD09_002840"
     CDS             123..>366
                     /locus_tag="OUD09_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCT9694024.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDR"
BASE COUNT          123 a           73 c           72 g           98 t
ORIGIN      
        1 ttttatataa taaaatgcaa tacttttcga gagtgtaaaa tattttgtgt aaatagaaaa
       61 aaggaagtcc cttctgtaga atagagttac cacaacacat tcacagaaaa gaggacttcc
      121 atatgaacga ttttactaca gaaattctaa agactctagc gaacaaaggc gatttgaatg
      181 aattattccg tgtccatttg gaaaaagctg tcaatacgct tctcaaaacg gagttaacgg
      241 ctttcctcga ttacgaaaag tacgatcgca ttggttttaa cacggggaat tctcgtaacg
      301 gctcctatga ccgtacggtc aagaccgagt acggggaact tcatctccag attccgcgcg
      361 accgca
//
LOCUS       DAKBVG010000437          361 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.452, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000437 DAKBVG010000000
VERSION     DAKBVG010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.452"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(14..101)
                     /locus_tag="OUD09_002841"
     tRNA            complement(14..101)
                     /locus_tag="OUD09_002841"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(65..67),aa:Ser,seq:gct)
     gene            complement(126..199)
                     /locus_tag="OUD09_002842"
     tRNA            complement(126..199)
                     /locus_tag="OUD09_002842"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(163..165),aa:Ile,seq:gat)
     gene            complement(213..283)
                     /locus_tag="OUD09_002843"
     tRNA            complement(213..283)
                     /locus_tag="OUD09_002843"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(249..251),aa:Gly,seq:tcc)
     gene            complement(285..360)
                     /locus_tag="OUD09_002844"
     tRNA            complement(285..360)
                     /locus_tag="OUD09_002844"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(325..327),aa:Asp,seq:gtc)
BASE COUNT          102 a          105 c           84 g           70 t
ORIGIN      
        1 caaaatattg aaacggagaa ggagggattt gaaccctcgc gccggtttcc cgacctacac
       61 ccttagcagg ggcgcctctt cagccacttg agtacttccc caaaaccaat aagattttgt
      121 actaatgggc ctaaatggac tcgaaccatc gacctcacgc ttatcaggcg tgcgctctaa
      181 ccagctgagc tataggccca taaaaaacta aaagcgggtg acgagaatcg aactcgcgac
      241 aacagcttgg aaggctgtgg ttttaccact aaactacacc cgcatggcgg ttccgacggg
      301 aatcgaaccc gcgatctcct gcgtgacagg caggcatgtt aacccctaca ccacggaacc
      361 a
//
LOCUS       DAKBVG010000438          361 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.453, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000438 DAKBVG010000000
VERSION     DAKBVG010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.453"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(<1..>361)
                     /locus_tag="OUD09_002845"
     CDS             complement(<1..>361)
                     /locus_tag="OUD09_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCT9694025.1"
                     /translation="GLYYSPVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAP
                     QEYLSRAYNDAPKYCANIVNTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLA
                     ELKGYQVTTGEPVCQTRR"
BASE COUNT          116 a           70 c           67 g          108 t
ORIGIN      
        1 gacgacgggt ctggcaaaca ggttcgccag tggtaacctg atatcctttt agctctgcta
       61 aacaaacact aagcccattt gtaaaaaaag ttaaatcatt gcgataatct tgaatacatc
      121 gagcaggaat ttctccaata ataatgacct cattattttt cagttgagta tttacgatat
      181 ttgcacaata tttgggagca tcgttatatg cccgtgaaag atattcctgt ggtgcataaa
      241 ctttaaaact aagatatggc tctaacaatt ctgttccagc ttttctaaag gcttgctcca
      301 gtacaatagg agtaagcatc cgaaaatctg ctggagtact aacagggcta tagtataaac
      361 c
//
LOCUS       DAKBVG010000439          348 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.454, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000439 DAKBVG010000000
VERSION     DAKBVG010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.454"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            36..>348
                     /locus_tag="OUD09_002846"
     CDS             36..>348
                     /locus_tag="OUD09_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9694026.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VL"
BASE COUNT          156 a           48 c           54 g           90 t
ORIGIN      
        1 ttaataggaa cgtatattcg aaaggaatag aaataatgtg gttagaaaaa ataaatgaca
       61 aaaaatttaa gtacaccgaa cgttatacgg atcccttaac tgaaaaaaag agaaaagtgt
      121 ccgttacttt atctagcaat tcacgccaag cttggaatca agctaactta cttttgaatg
      181 aaaaaatcgc agaaaaaata aaacgaaatg aagaattacc actaactttt ggagaattaa
      241 aaactaaatg ggatgaaaaa tacaagccaa ctgtaaaaga aagttcctat cgaacaactc
      301 aggtttatct atcattaatt tcaaaatata taaaagatga tgtacttg
//
LOCUS       DAKBVG010000440          335 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.455, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000440 DAKBVG010000000
VERSION     DAKBVG010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.455"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(<1..216)
                     /locus_tag="OUD09_002847"
     CDS             complement(<1..216)
                     /locus_tag="OUD09_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCT9694027.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIV"
BASE COUNT          129 a           59 c           41 g          106 t
ORIGIN      
        1 tacaatcgca ccaataaaaa aattaatacc taaaatataa gaaattgcta ctcctggcaa
       61 aacagcatga gaaatagcat cacccataag agacattcct cttaaaataa taaaacaacc
      121 tactgttcca gaaacaatcc ctataactac agatgtaatt agagcattct gtaaaaagtg
      181 aaagtcatac agaccattaa taaaattttg aagcatatta ttccccttgt atattagagt
      241 tagggtttta accttccggt tttgattgga taaccctatg aaccatcgtt attttaattt
      301 ggtattaact catttctttt gagataatat attta
//
LOCUS       DAKBVG010000441          334 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.456, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000441 DAKBVG010000000
VERSION     DAKBVG010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.456"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(<1..>334)
                     /locus_tag="OUD09_002848"
     CDS             complement(<1..>334)
                     /locus_tag="OUD09_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HCT9694028.1"
                     /translation="KQGYSNSDIYAELYYQYPIFLNVNEKYKKMKLSSKLVYMLLKDR
                     NRLSLKNNLYDENQKIFLISEIKNLVEISGMSKNTILSALDELQKVKLLVKRKNGFNK
                     SKMQMFPNF"
BASE COUNT          108 a           42 c           38 g          146 t
ORIGIN      
        1 aaaaatttgg gaacatttgc attttacttt tattgaagcc attttttctt ttcacaagaa
       61 gtttaacttt ttgaagttca tctagtgctg ataagattgt attttttgac attcccgaaa
      121 tttctacgag atttttgatt tcagatatca aaaaaatttt ttgattctca tcatacaagt
      181 tatttttcaa tgaaagtcta tttctatctt ttagtaacat ataaactaac ttagaactta
      241 gtttcatttt cttatatttt tcatttacgt ttaaaaaaat aggatattga tagtataatt
      301 ctgcatatat atcagaatta gaatagcctt gctt
//
LOCUS       DAKBVG010000442          332 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.457, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000442 DAKBVG010000000
VERSION     DAKBVG010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.457"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            <1..>332
                     /locus_tag="OUD09_002849"
     CDS             <1..>332
                     /locus_tag="OUD09_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9694029.1"
                     /translation="IILSFLGKVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAE
                     YTIHIEVPPNPFWASIGLSVSPLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQ
                     GLYGWNVT"
BASE COUNT          103 a           53 c           78 g           98 t
ORIGIN      
        1 attatacttt ctttcttagg gaaagtacaa atggaagtga ttagtgcact gttgcaagaa
       61 aagtatcatg tggagataga actaaaagag cctacagtca tttatatgga gagaccgtta
      121 aaaaatgcag aatataccat tcacatcgaa gtgccgccaa atcctttctg ggcttccatt
      181 ggtttatctg tatcaccgct tccgttggga agtggaatgc agtatgagag ctcggtttct
      241 cttggatact taaatcaatc atttcaaaat gcagttatgg aagggatacg ctatggttgc
      301 gaacaaggat tatatggttg gaatgtgacg ga
//
LOCUS       DAKBVG010000443          322 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.458, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000443 DAKBVG010000000
VERSION     DAKBVG010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.458"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            91..>322
                     /locus_tag="OUD09_002850"
     CDS             91..>322
                     /locus_tag="OUD09_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCT9694030.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMR"
BASE COUNT           93 a           57 c           62 g          110 t
ORIGIN      
        1 agactgtaga caaagtccat tttatggatg atgtctacag tcttttcttt tataatagat
       61 ttatgaaaac gaatgcaagg agtgatagct atgatgtcta aaaacaatac gaatggtaga
      121 aatcaatttg cgatgcttac gattgatgat ctagttcctc aagatcatct cgttcgaaag
      181 attgatgccg cgcttgattt tgaatttatt tatccaatcg tcgaagccac ttactccgat
      241 ttaggtcgcc caagtatcga tccagtgatt ctgatcaaat tggtgtttat tcagtattta
      301 ttcggtatcc gttccatgcg ac
//
LOCUS       DAKBVG010000444          321 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.459, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000444 DAKBVG010000000
VERSION     DAKBVG010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.459"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(69..321)
                     /locus_tag="OUD09_002851"
                     /pseudo
     CDS             complement(69..321)
                     /locus_tag="OUD09_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT           79 a           78 c           51 g          113 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat g
//
LOCUS       DAKBVG010000445          320 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.460, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000445 DAKBVG010000000
VERSION     DAKBVG010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.460"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            239..>320
                     /locus_tag="OUD09_002852"
     CDS             239..>320
                     /locus_tag="OUD09_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HCT9694031.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLS"
BASE COUNT          127 a           43 c           62 g           88 t
ORIGIN      
        1 tctcaaataa gtatgtaccc aatgagctaa ttgtcagttt aaaggctaca caaagaattt
       61 caacagagaa aaataaaccg caaaatttat tgggtgtaaa agttatggtg gaaaaagatt
      121 tgactcttag gacaaatgaa agcgattgta ataagcgaga tcaagggttc tgttgcaaag
      181 ttttccaaaa aatctatttt agtgtaaaat tgagaaaaaa gacagagagg acagagtaat
      241 gaatcatttt aaaggcaaac aattcaaaaa agacgtcatt attgtcgctg ttggttacta
      301 cctgcgttac aatctaagct
//
LOCUS       DAKBVG010000446          317 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.461, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000446 DAKBVG010000000
VERSION     DAKBVG010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.461"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            <1..>317
                     /locus_tag="OUD09_002853"
     CDS             <1..>317
                     /locus_tag="OUD09_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HCT9694032.1"
                     /translation="QKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGV
                     QLGKQPGTILQVETEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYL
                     SYPR"
BASE COUNT          119 a           62 c           65 g           71 t
ORIGIN      
        1 caaaaagaac catattttga aggaaaagcc caagtaatcg ctcaaaatgg cgcatttgat
       61 gcgaagcttg atccaaatga aacccaagcg acacaagaag catttgaagc ctatttaaaa
      121 gaaaaaggtg ttcaattagg aaaacagcca ggtacgatcc ttcaagtcga aacagagaaa
      181 aaaagtgcgg ctagcccacg tcttttttct ttatcgagcc ttcagtctaa aatgaatcaa
      241 ctcatgaaag ctagtgccaa agatactttg gaagccatgc aagggttgta tgaaggaaaa
      301 tatctaagct atccgcg
//
LOCUS       DAKBVG010000447          309 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.462, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000447 DAKBVG010000000
VERSION     DAKBVG010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.462"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            1..309
                     /locus_tag="OUD09_002854"
                     /pseudo
     CDS             1..309
                     /locus_tag="OUD09_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          122 a           61 c           59 g           67 t
ORIGIN      
        1 attcaaaaga attcaaacaa caaatcgtcg atctctatct cgctggtaag cctcgcgcag
       61 aaattattcg agagtatgag cttacgcctt cttctttcga taaatggatg aagcaagcac
      121 aatcaacggg ctcattcaaa gaaagagaca acttaacacc agaacaagca gaattgatcg
      181 cactaagaaa gaaaaataag caactcgaaa tggagaatga tattttaaag caagcggcgc
      241 tgatattcgg acgaaaagac aagtaattga tgccaacaag cataaatatt ccatatcagc
      301 gatgtgcaa
//
LOCUS       DAKBVG010000448          304 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.463, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000448 DAKBVG010000000
VERSION     DAKBVG010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.463"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            <1..>304
                     /locus_tag="OUD09_002855"
     CDS             <1..>304
                     /locus_tag="OUD09_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCT9694033.1"
                     /translation="KKALSKRGIQISRRKISRIMKNRGLKSSYTVAYFKVHHSTCNEA
                     KTTNVLNRKFLRDNPLEAIVTDLTYVRVGKKWNYVCFILDLFNREILGYSCGEHKDA"
BASE COUNT          114 a           50 c           57 g           83 t
ORIGIN      
        1 aaaaaaagcc ttatcaaaac gaggcattca gatcagccga cgaaaaatta gtagaatcat
       61 gaaaaataga ggattaaaat cgagctatac tgttgcttat tttaaagtac atcattctac
      121 ttgcaatgaa gccaaaacga caaacgtatt gaatcgtaaa ttcttaagag acaacccatt
      181 agaagcgatc gtaacagact tgacttatgt acgagtcggg aaaaaatgga attatgtctg
      241 tttcattttg gatctgttca atcgagaaat tctcggctat tcttgtggag aacataaaga
      301 tgcc
//
LOCUS       DAKBVG010000449          299 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.464, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000449 DAKBVG010000000
VERSION     DAKBVG010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.464"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(122..>299)
                     /locus_tag="OUD09_002856"
     CDS             complement(122..>299)
                     /locus_tag="OUD09_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCT9694034.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT           95 a           72 c           43 g           89 t
ORIGIN      
        1 aatggtggcc aattagctga ccttcggtat tagctcaaaa aacggcaaaa tatgaggagc
       61 tatttttatt tggctattct tattacttga tgcagaacag cttttcacaa cctcttttcg
      121 tctatttttt attttcgaga tttctctttc ctttaaaaac aactgtttga aagaagaacg
      181 cacatccttg aaaaacaagc ccgctaaccg ttacacctaa ccagccatac tgctgccaca
      241 ttgtcgtccc tagcaatgaa ccagccgcac ctccaataaa gtacataaac ataaataca
//
LOCUS       DAKBVG010000450          291 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.465, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000450 DAKBVG010000000
VERSION     DAKBVG010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.465"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            28..>291
                     /locus_tag="OUD09_002857"
     CDS             28..>291
                     /locus_tag="OUD09_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCT9694035.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGW"
BASE COUNT          112 a           51 c           50 g           78 t
ORIGIN      
        1 ccaaagaatt ataaggagtg agtacaaatg acctataccc atcttacaac ggatgaactt
       61 gtaataatag agtcttattt caaaatgaat caatctgttg ctaaaactgc ccattgcttg
      121 aatcgttcaa gacaaacgat ccataaagta tatctgttct tcaagcaagg aaaatcagcc
      181 ttagaatatt atcaacagta taagaaaaac aaatcaaact gtggtagacg tccgcttgtt
      241 ttacccgagg aacaatcaga atatattcaa agaaaggttg ttcaaggatg g
//
LOCUS       DAKBVG010000451          280 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.466, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000451 DAKBVG010000000
VERSION     DAKBVG010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..280
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.466"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(<1..>280)
                     /locus_tag="OUD09_002858"
     CDS             complement(<1..>280)
                     /locus_tag="OUD09_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9694036.1"
                     /translation="ETQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINLYYSNK
                     NYFKIPAAKTRMKKDVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPER"
BASE COUNT           95 a           49 c           42 g           94 t
ORIGIN      
        1 tatctttctg gatcaacgaa agtatacttt atatagtcat aacgccgatg atcgctacgt
       61 gcgtccggca cgtcagtcac gatatcaaac aaaaagtata catccttctt cattctagtt
      121 ttcgcagcag gaattttgaa atagttctta ttagaatagt agagattgat taataagcta
      181 tcttcgattg ctaaaaagaa aacttctgaa ttccatactt tataaaaatc tttgataaat
      241 ctattcgaag ggtcaaattt aaaccataat tgtgtctcat
//
LOCUS       DAKBVG010000452          274 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.467, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000452 DAKBVG010000000
VERSION     DAKBVG010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.467"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(<1..243)
                     /locus_tag="OUD09_002859"
     CDS             complement(<1..243)
                     /locus_tag="OUD09_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002384409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCT9694037.1"
                     /translation="MEVIIMNVGFIGAGKVGCSFGKYFQEHKIQVTGFYSKSEDSSLA
                     ASNFTSSKQYLNLRELVDENDTIFITTPDGQIQEVWQ"
BASE COUNT           86 a           60 c           36 g           92 t
ORIGIN      
        1 ttgccatacc tcctgaattt gaccatcagg agtcgtaatg aaaatagtat cattttcatc
       61 tactaattct cttaagttta aatattgttt actagatgta aaattcgaag ctgctaatga
      121 cgagtcttcg cttttactat aaaatcctgt tacttgtatt ttatgttcct ggaaatactt
      181 cccgaatgaa caacctacct tgccggctcc aataaaacca acgttcataa ttatcacctc
      241 cataaagaag tgagactaca agtcccattc ttac
//
LOCUS       DAKBVG010000453          268 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.468, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000453 DAKBVG010000000
VERSION     DAKBVG010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.468"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
BASE COUNT           72 a           52 c           53 g           91 t
ORIGIN      
        1 agcctagcgt gagcggggcg gattgactgg caagtagatg ggtagctggc gtatgtccta
       61 aaaactgctt ctttccaagc aactcttcat aggcaaaaaa ctggaaagtc cactaacaag
      121 cccattcaga attccatttc aaaattttgt tgcctttttt gctgattttc aatcatttgt
      181 tgtgtgaatt cataatcctt tttagcacga tacattttta catcgtcatt cattatgcga
      241 ctgatttgat agtgaatctg tttagcag
//
LOCUS       DAKBVG010000454          265 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.469, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000454 DAKBVG010000000
VERSION     DAKBVG010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.469"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            <1..>265
                     /locus_tag="OUD09_002860"
     CDS             <1..>265
                     /locus_tag="OUD09_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HCT9694038.1"
                     /translation="KAGFVTEDNLYLDSTHIKANANKHKFTKEMTHDEAKAYQDELED
                     EINRQRIEAGKRPFTLDLEKEVKLKERKISKADPESGYYVKGER"
BASE COUNT          106 a           44 c           61 g           54 t
ORIGIN      
        1 aaagcaggtt ttgtaacaga ggataatttg tatctggatt ctacccacat caaagctaac
       61 gccaataaac ataagtttac gaaagaaatg acccatgatg aagcgaaagc ctatcaggat
      121 gaattagaag atgagatcaa tcgtcaacga atagaggctg gaaaacgccc ttttactttg
      181 gacttagaaa aggaagtgaa gctgaaagaa agaaaaataa gtaaagctga tccggaaagc
      241 ggctactacg tcaaaggcga acgag
//
LOCUS       DAKBVG010000455          262 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.470, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000455 DAKBVG010000000
VERSION     DAKBVG010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..262
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.470"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(57..173)
                     /locus_tag="OUD09_002861"
     CDS             complement(57..173)
                     /locus_tag="OUD09_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HCT9694039.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
BASE COUNT           99 a           55 c           34 g           74 t
ORIGIN      
        1 gcatcaacat gagctaaaac tccaatatta ataattttca tgtgattttc ctccattcaa
       61 aagcccaaaa gggcataaaa atcccagtga taaatactct tatcactggg atttttatgc
      121 ataaccatag gcatacaaag catacagata ttctccggat actttagaat cacatgataa
      181 aggtattctt aaactgggtg caaaaaacta agccctccta aaaaaggaca tccaattatt
      241 tgttcccact atcaaattga ca
//
LOCUS       DAKBVG010000456          260 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.471, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000456 DAKBVG010000000
VERSION     DAKBVG010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..260
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.471"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            <1..>260
                     /locus_tag="OUD09_002862"
     CDS             <1..>260
                     /locus_tag="OUD09_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCT9694040.1"
                     /translation="KKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNLDSV
                     TAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGK"
BASE COUNT          107 a           30 c           52 g           71 t
ORIGIN      
        1 ataaaaaaca aataaaaaga aatgttatga aattatctgg tggacagcaa caaagagtag
       61 ctattgctag agcattggta tcagatgctc caataatact agctgatgag cctactggta
      121 atctagacag tgttactgct ggagaaataa ttaatatatt aaagacatta gctaaagata
      181 gaaataaatg tgtaatagtt gtaacacata gtaaggaagt agcagattct gcggatatca
      241 ttttagaact aagtggtaaa
//
LOCUS       DAKBVG010000457          259 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.472, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000457 DAKBVG010000000
VERSION     DAKBVG010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.472"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
BASE COUNT           93 a           30 c           47 g           89 t
ORIGIN      
        1 aatgaacaaa cataatagaa aaataacgaa taacaaagaa ctgatcttgt tactaatagg
       61 gatttggggt atctttttac tctttaatat gtatatgccg actatgagag cggatgacgt
      121 agtttatgca agtagattag atgaacttgg ctatttgggt gcttcgatag aacattataa
      181 gacatggagt tcaagaatta ttatagaact attcttaatg ttcttttcta aacactttat
      241 gctatggaaa ttattaaat
//
LOCUS       DAKBVG010000458          252 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.473, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000458 DAKBVG010000000
VERSION     DAKBVG010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.473"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            <1..160
                     /locus_tag="OUD09_002863"
     CDS             <1..160
                     /locus_tag="OUD09_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HCT9694041.1"
                     /translation="KRYSRRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTD
                     TLVSMFTE"
BASE COUNT           95 a           40 c           38 g           79 t
ORIGIN      
        1 taaaagatac agccgtagaa aagagcagtt tcaaaatgaa gaatcactag aacgctttct
       61 agtcagcatt tttgatacat acaatcaaaa atttctaaac agaagccata aaggttttca
      121 acaggtaacc gatacattag tttcaatgtt tactgagtaa ctaattattt tgcaggagga
      181 caatttattt acacaaaatt attgacgctc ccaaactgat cattaagttt ggcttttatg
      241 tcataattga at
//
LOCUS       DAKBVG010000459          248 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.474, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000459 DAKBVG010000000
VERSION     DAKBVG010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.474"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            82..>248
                     /locus_tag="OUD09_002864"
     CDS             82..>248
                     /locus_tag="OUD09_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HCT9694042.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDG"
BASE COUNT           87 a           39 c           48 g           74 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaa
//
LOCUS       DAKBVG010000460          247 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.475, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000460 DAKBVG010000000
VERSION     DAKBVG010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.475"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            <1..>247
                     /locus_tag="OUD09_002865"
     CDS             <1..>247
                     /locus_tag="OUD09_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCT9694043.1"
                     /translation="KPEGRKAVDIENSINEWLQSVPKNLFKSITFDCGKEFSNWKSIS
                     NTNDIDIYFADPGTPSQRGLNEHSNGLLRKDGLPKEME"
BASE COUNT           93 a           44 c           46 g           64 t
ORIGIN      
        1 gaaaccagaa ggccgtaagg cagttgatat tgaaaattcg attaatgaat ggcttcaatc
       61 cgtacccaaa aatcttttca aatcaattac ctttgattgt ggaaaagaat tctctaattg
      121 gaaaagtatt agtaatacga atgatattga tatttatttc gcagacccag gaacgccttc
      181 ccaacgggga ttaaatgaac attcaaacgg actcctccga aaagatggac taccaaaaga
      241 aatggaa
//
LOCUS       DAKBVG010000461          245 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.476, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000461 DAKBVG010000000
VERSION     DAKBVG010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.476"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
BASE COUNT           78 a           45 c           44 g           78 t
ORIGIN      
        1 aaatatcagg gtcactgctt attttcctcc ctaaaacttc tgaaagatcc ggcagtttta
       61 gatcactgga caagactcaa agattttcta tgtgtgtttc agttatgtaa tattcactgt
      121 ttgcaaaaaa tactgttaga ttaactagat gaagcgtctt gtcctcatcc gatctagatt
      181 attcgatatt cctcgacaat aaacgattac tcagtgaggg caatcagagg aacggataat
      241 gataa
//
LOCUS       DAKBVG010000462          243 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.477, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000462 DAKBVG010000000
VERSION     DAKBVG010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..243
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.477"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            <1..>243
                     /locus_tag="OUD09_002866"
     CDS             <1..>243
                     /locus_tag="OUD09_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT9694044.1"
                     /translation="LNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEK
                     DSNAFEESLESVSGIKYIIKVMTYSIMLGGMVVLSLI"
BASE COUNT           93 a           21 c           44 g           85 t
ORIGIN      
        1 ttaaataatt cagaaaataa tgaaattgca aataaaattt taatgtattc tggtagtgca
       61 gaatctacag acttagcctt aaacaaattg aaagagctta aaattgatga gtcaaagtat
      121 tttgttgaaa aagattctaa tgcgtttgaa gagtctttag agtcagtgag tggaataaaa
      181 tatataataa aagtaatgac ttattctatt atgttaggtg ggatggttgt tctttcatta
      241 atc
//
LOCUS       DAKBVG010000463          235 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.478, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000463 DAKBVG010000000
VERSION     DAKBVG010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.478"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(<1..202)
                     /locus_tag="OUD09_002867"
     CDS             complement(<1..202)
                     /locus_tag="OUD09_002867"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCT9694045.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQ"
BASE COUNT           69 a           34 c           31 g          101 t
ORIGIN      
        1 tttgaggtct atatttttta actacaatcg accttaaatt taattgtttc attagctttt
       61 ggacatgttt aagagatact gaaatccctt cttttaataa aacttgatgg atctttgtgg
      121 caccatagcg ttgtttattc gtaaaaaaaa ttgttgaaat cttctttttc aacttattat
      181 ttctgatttc tgtagctgtc aacggtttat tcttataaaa ataatagaca cttct
//
LOCUS       DAKBVG010000464          233 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.479, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000464 DAKBVG010000000
VERSION     DAKBVG010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.479"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            22..>233
                     /locus_tag="OUD09_002868"
     CDS             22..>233
                     /locus_tag="OUD09_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCT9694046.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEI"
BASE COUNT           87 a           44 c           44 g           58 t
ORIGIN      
        1 atacaaggag agtgaacata aatgacttat acccatctta caccaaacga gcttgtaatg
       61 atagaagcat attttcatca agaaactccg gttgctatcg ttgcgaagca gcttaaacgt
      121 ggacgccaaa caatttacaa tgtctataac tttctcaaat gtggtggaac agcacttgaa
      181 tactttgaac aatacaaaga aaataagcga cgttgtggga gaaccgaaat cat
//
LOCUS       DAKBVG010000465          226 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.480, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000465 DAKBVG010000000
VERSION     DAKBVG010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..226
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.480"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            <1..180
                     /locus_tag="OUD09_002869"
     CDS             <1..180
                     /locus_tag="OUD09_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9694047.1"
                     /translation="TKKMMETIGPALSAQRAVMQNIDTEEFRRATERMKQSLSPKQAL
                     KSDYKIADDIEDSNK"
BASE COUNT           93 a           37 c           42 g           54 t
ORIGIN      
        1 actaaaaaaa tgatggagac aatcggtccg gcattatccg ctcaacgagc agtcatgcaa
       61 aacattgata ctgaggagtt cagacgagca actgagagaa tgaagcagtc tctatcacca
      121 aagcaagcat tgaaatccga ttataaaatt gctgatgata ttgaagatag taataaataa
      181 agagtccaga actttttcca attaataaac tattaaaaga attaga
//
LOCUS       DAKBVG010000466          224 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.481, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000466 DAKBVG010000000
VERSION     DAKBVG010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.481"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            <1..>224
                     /locus_tag="OUD09_002870"
     CDS             <1..>224
                     /locus_tag="OUD09_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HCT9694048.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAA
                     ESQTPAKPAAEPTVDTESAGSSSEGVFQFK"
BASE COUNT           70 a           47 c           58 g           49 t
ORIGIN      
        1 ccgtcacctg ttacaggaac ggttaaaaat gtgattgttc cagaaggtac cgtagcaaat
       61 gttggggatg tattagtaga aatcgatgca ccaggacacg aagacaatga aggggacagt
      121 ggtgtagccg cagaatccca aactccagct aaaccggctg ctgaacccac tgttgataca
      181 gaatcagctg gatcgtcaag cgaaggagtc ttccaattta agtt
//
LOCUS       DAKBVG010000467          223 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.482, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000467 DAKBVG010000000
VERSION     DAKBVG010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..223
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.482"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            <1..197
                     /locus_tag="OUD09_002871"
     CDS             <1..197
                     /locus_tag="OUD09_002871"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCT9694049.1"
                     /translation="SVRGLAKEYGLSEATIYKWKNLYLPDQSTGLTGKEVAELRKENA
                     RLNEELEILKKAAAIFSRKT"
BASE COUNT           83 a           35 c           45 g           60 t
ORIGIN      
        1 gttctgttcg agggttagcg aaagaatatg gcttatctga agcaacgatt tacaaatgga
       61 agaatttata tttacctgat cagtccacag gactgactgg aaaagaagta gctgaactga
      121 gaaaagaaaa tgctcgttta aatgaggaac ttgaaatctt aaaaaaagcc gcagccatat
      181 tctctcggaa aacctaaatt cgcttgttca atttattgaa aaa
//
LOCUS       DAKBVG010000468          216 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.484, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000468 DAKBVG010000000
VERSION     DAKBVG010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..216
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.484"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            <1..>216
                     /locus_tag="OUD09_002872"
     CDS             <1..>216
                     /locus_tag="OUD09_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HCT9694050.1"
                     /translation="IAKNCQVSLTTVIRTLKEFKSYLPKQSKRILPRVLMVDEFRSHA
                     SIEDKMSFICADGETGQLIDVLPTRKL"
BASE COUNT           66 a           39 c           45 g           66 t
ORIGIN      
        1 ttattgcgaa aaattgtcag gtatctttga ccaccgttat tcgtacgttg aaagagttta
       61 aaagctactt accaaagcaa tctaagagga ttctccccag agtattgatg gttgatgaat
      121 ttcgttcgca cgcttccata gaagataaaa tgagctttat ttgcgcagat ggcgaaacag
      181 gacaattaat tgatgtcttg cctacgcgta aattgc
//
LOCUS       DAKBVG010000469          215 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.485, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000469 DAKBVG010000000
VERSION     DAKBVG010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.485"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(<1..>215)
                     /locus_tag="OUD09_002873"
     CDS             complement(<1..>215)
                     /locus_tag="OUD09_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCT9694051.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTS"
BASE COUNT           66 a           40 c           42 g           67 t
ORIGIN      
        1 ggaagttgtg aatcgttgaa tacgcaagaa atcagcgatt cttaatgcat cgtttcgatc
       61 agttttattc tcatcaaaca tgcgactaaa ttgtttcacc cgaaaaggat tttcaatcgt
      121 tacaagtgtg ttgagcttct ttagtttctc atcttcatga aaaaacatgg aaggatggaa
      181 gctgtacatg gacgttgact ccatgccaat cacaa
//
LOCUS       DAKBVG010000470          213 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.486, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000470 DAKBVG010000000
VERSION     DAKBVG010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.486"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            10..>213
                     /locus_tag="OUD09_002874"
     CDS             10..>213
                     /locus_tag="OUD09_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9694052.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELE"
BASE COUNT           86 a           23 c           46 g           58 t
ORIGIN      
        1 ggatgaaata tgaataacga aactatcatt ttaggaaaaa aatatatttg ccaacctatt
       61 ggcttgaaac aaaaagtggt tggagaagta atcagtaaat tagaaaattg tgttgtgatc
      121 tgcgtagacg aatatcatga cgtcgatcgc aatgaaatat tagaaaaatg cggaaaagta
      181 gtggcaaaat atactcacgt ttatgagttg gaa
//
LOCUS       DAKBVG010000471          209 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.487, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000471 DAKBVG010000000
VERSION     DAKBVG010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.487"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(107..>209)
                     /locus_tag="OUD09_002875"
     CDS             complement(107..>209)
                     /locus_tag="OUD09_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011282421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HCT9694053.1"
                     /translation="FYQALKYARIDDLNNISFEQFLSLFNSYKLFNR"
BASE COUNT           77 a           28 c           36 g           68 t
ORIGIN      
        1 gccacctaaa aaggagcgat ttacatatga gttatgcagt ttacgggcca agattgttta
       61 agcaaaataa gataagttaa gggatgcagt ttatgcaccc cttaacttat ctattaaata
      121 atttatagct attgaaaaga gacaagaatt gttcaaagct aatattgttt aaatcgtcaa
      181 ttcttgcata ttttaaggct tgataaaat
//
LOCUS       DAKBVG010000472          206 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.488, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000472 DAKBVG010000000
VERSION     DAKBVG010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..206
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.488"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
BASE COUNT           91 a           26 c           34 g           55 t
ORIGIN      
        1 ccttaggcag cttttgctgt gtagaaagga ataaataata acgaaagttc aaaagctaat
       61 gccgactatt ctagacatta aagaagacga aaacgaacga taaagaaaga aaagttatga
      121 ataatcgttt attgaaaaaa acttgaaaaa atacaaaaaa ttcgttgacc aagctaaatt
      181 tagttggtat attattactt gtccaa
//
LOCUS       DAKBVG010000473          206 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.489, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000473 DAKBVG010000000
VERSION     DAKBVG010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..206
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.489"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            80..>206
                     /locus_tag="OUD09_002876"
     CDS             80..>206
                     /locus_tag="OUD09_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HCT9694054.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFS"
BASE COUNT           86 a           21 c           41 g           58 t
ORIGIN      
        1 ctcaaaattt tttgattaga aaaataggta tattgaatca gtgacataaa agtatttata
       61 gtttgtaggg ggaatcgtaa tggaagaaaa aaatagacta aatgctaaac aatgttcaga
      121 tcaagagctt aaaaaaatta aaggaggtgc tggaacaaaa cctcaaggaa aaccggcatc
      181 aaatttagtg gaatgtgttt tttcat
//
LOCUS       DAKBVG010000474          205 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.490, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000474 DAKBVG010000000
VERSION     DAKBVG010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.490"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            complement(<1..>205)
                     /locus_tag="OUD09_002877"
     CDS             complement(<1..>205)
                     /locus_tag="OUD09_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCT9694055.1"
                     /translation="ILANNINPLIEEYANQRHSISKELFFKRANSKLQDKIRNGDFKY
                     GEKERMIDSLKQLLDEMDLAYKDI"
BASE COUNT           56 a           32 c           32 g           85 t
ORIGIN      
        1 aaatatcttt ataggctaag tccatttcgt ctaatagctg ttttaaagaa tcaatcattc
       61 tttctttttc accgtatttg aaatcaccat ttcttatctt gtcttgaagt ttactgttgg
      121 ctcttttgaa aaatagctct ttagagatag agtgtctttg gttagcgtac tcttcaatca
      181 gaggattgat attattagca agtat
//
LOCUS       DAKBVG010000475          202 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.denovo.491, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000475 DAKBVG010000000
VERSION     DAKBVG010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..202
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.denovo.491"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            <1..125
                     /locus_tag="OUD09_002878"
     CDS             <1..125
                     /locus_tag="OUD09_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HCT9694056.1"
                     /translation="YGNGVYCTKNKCTVDWAKATTCIAGMSIGGFLGGAIPGKC"
     gene            127..>202
                     /locus_tag="OUD09_002879"
     CDS             127..>202
                     /locus_tag="OUD09_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HCT9694057.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYS"
BASE COUNT           74 a           22 c           44 g           62 t
ORIGIN      
        1 attatggaaa tggagtgtat tgcactaaaa ataaatgtac ggtcgattgg gccaaggcaa
       61 ctacttgtat tgcaggaatg tctataggtg gttttttagg tggagcaatt ccagggaagt
      121 gctaaaatga aaaaaaatgc taagcaaatt gttcatgaat tatataatga tatatctata
      181 agtaaagatc ctaaatattc tg
//
LOCUS       DAKBVG010000476         3446 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.guided.220, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000476 DAKBVG010000000
VERSION     DAKBVG010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.guided.220"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            <1..352
                     /locus_tag="OUD09_002880"
     CDS             <1..352
                     /locus_tag="OUD09_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HCT9694058.1"
                     /translation="GEEIYNQAYESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKV
                     DSSLAAMQFMLVARDHGYETNPIGGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGY
                     ESVRLAPEKITTFE"
     gene            706..2184
                     /gene="msr(C)"
                     /locus_tag="OUD09_002881"
     CDS             706..2184
                     /gene="msr(C)"
                     /locus_tag="OUD09_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HCT9694059.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            2231..2677
                     /locus_tag="OUD09_002882"
     CDS             2231..2677
                     /locus_tag="OUD09_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HCT9694060.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2876..3391)
                     /locus_tag="OUD09_002883"
     CDS             complement(2876..3391)
                     /locus_tag="OUD09_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCT9694061.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
BASE COUNT         1100 a          581 c          721 g         1044 t
ORIGIN      
        1 tggcgaagaa atctataacc aagcttacga atcagggaag atgccaaaag aagtcagaga
       61 tcaacaatta gctgcgatca ttccttatta caagagtttt tcaagagaag aaatgaacga
      121 tgtagtcaaa gtcgattcta gcttagcggc tatgcaattc atgttagttg cgcgtgatca
      181 tggctatgaa acaaatccaa ttggtggatt cgaagcagat cagttggcag aggcatttgg
      241 tttggataaa gaccgttatg ttccagttat tattttatca ataggaaaag cggtagaaga
      301 aggttacgag tctgttcgtt tagcaccaga aaagatcact acgttcgaat aaaaggtttt
      361 cagttactat tcagtagcta ttgctttttt tgaatcgtgt gtggtaacct aataaaaaag
      421 gaggtcggaa aaatgactgc atcgatgaaa ttacgtttcg aacttttgaa taacaactaa
      481 ataaattcaa aagacatgta taaacaacaa tttcatggat ggagtatgtc cattttttcg
      541 aacgacagta gaataggtga aagctatgac agatttggtg atttagtcat agttatttcg
      601 ttcagagtag gaaaagatgt gatgcagttt ttttgcaaac agatttttct gctctttttt
      661 agtatcgata attgttgttt tacatgaaat aggagtgtaa aacatatgga aaatttagca
      721 gtaaatataa caaatctaca agtcagcttt ggcaaccagc tagaactatc tattgattct
      781 cttcgtgtct atcagcaaga tcgtataggg atcataggag aaaatggtgt cggtaaatcg
      841 actttgctca aactaatagc cggtgaactt tttcccgatc atgggaaaat ccaaacagag
      901 atcaccttca actacctgcc tcaattaacc tatctcgctg aagcaaagga cctaaatttg
      961 gaattagcca gtcatttcca gttaagactg gaagaaactt cagagcggaa atggagcgga
     1021 ggggaagaac gaaagatcga gttgatacgt cttctttctt cttatgaaca agggatgctt
     1081 ctagacgagc caacaaccca tctagataga aaaagtattg atcgactgat tgaagagctt
     1141 cgttattatt atggtacgct ggtttttgtt agtcatgatc gctattttct agatgagcta
     1201 gcatcgaaaa tctgggaagt aaaagacgga gaaatccgag agttttcggg gaattatagt
     1261 gcctatctca ctcaaaagga attggagaaa aagactcagc tacgagaagc agagtcgatc
     1321 atgaaagaga aaaaacgatt ggaaaaatcg atccaagaaa agaaaaaaca agcggaaaag
     1381 ttggaaaaag tgtccagtaa aaagaaaaag caacaaatca gaccggatcg gttgtcttcc
     1441 tctaaacaaa aagacagtgt acaaaaagcc atccaaaaga atgcgaaaac attagagaga
     1501 agactccaaa aaataggaga aacaaccaag ccgcagcaga tgaaacaaat ccgttttcca
     1561 gtaccaaaat ctcttgaact ccacagccgc tatcccatca tgggacaaaa tgtccaattg
     1621 gaaagaagtg ggagaacatt actggtaaat ggtgattttc agttttcttt aggtaaaaaa
     1681 atcgcgattg tcggtgaaaa tggttcaggt aagacaacct tattagaaca tatccgcaaa
     1741 caaggagaag gaatccttct ctctccgaaa gtaagctttc aagtatatca gcaaaagggt
     1801 tatcaaatga catctgaaga atccatcatt cgttttgtca tgagacaaac agagttttca
     1861 gaatcgcttg tccgtagttt gctgaatcac ttagggtttg ctcaggaaac tttgacgaaa
     1921 ccgttatgta cattaagtgg gggagaagcg acccgtctga cgattgcttt gctttttact
     1981 aagccaagta atgtgttgct gttagatgaa ccgactaatt ttattgatat ggcaacgatc
     2041 gaagctttag agaagctaat gcaaatatat ccgggaacga ttttgtttac ttctcatgat
     2101 tcttactttg tcgagcgtac ggctgatgaa gtttatgaaa taaaagggca gaaaataaaa
     2161 aaagtactta cgagaaattt ttaaatgtgc tacgatgatt ttcgtgacaa atttaaagga
     2221 gtggtacaga ttgcctacaa ataaaaaaga aggaataatt tttacaacaa tgatgtgttt
     2281 tttgatggtc ttggggatga gcgcgtataa tttatggctg catcaagatc ttcatttatc
     2341 aagtttgttg attggtctag tgcctggatt cgtggtggct ttcattttgg atgtcttcat
     2401 catcggtgtg ctggcgaaaa aaattgcctt taaactacca gtaaatccag aaagcaaggt
     2461 ccaaatgatc ttaactattt catgcttgat ggttttagga atggtgacat gtatgtcttt
     2521 attcggtgtg atcatggaac aaggaatacc tgatcaatta ttctctgctt atatgaatgc
     2581 ttggaaaatg aatgtgattg ccgctttacc gttgcagttg atcatcgttg gcccgctttc
     2641 aagaagtgtg ctaaaagcaa ttcaaagtaa tgaatgatgg aacactgtta tttgaaaaaa
     2701 ggccttggca tgctttttgt catagtctct tgaatgcagt tctagaaaaa tagtgatccg
     2761 aacgataggt tttggcctgt cgttcggatc gctttttctt ttccaactgt attgctcttt
     2821 tattgaagat aatttgctct tcaatagata tttctagaaa ggttctgttt atcccttact
     2881 caaaaaactg ctgcatttgt tctcttactt gctgacacca ttggagttcc tgctgacaaa
     2941 gcataattct gcgctggatc acaacataac gtccaaattc ttgtttcttg tctagcgaga
     3001 ggtcattcaa ttttttttga tttttttgca tgattttgtt cagctgttgt tctctagtga
     3061 atagcagtcc agtagctgtt gttttatcga gcgtcgaaaa agcatagatt tttgctagaa
     3121 attcatcttt ttgcgtagga gcgggagttt cctctttgat ccattcctct acaagagcac
     3181 gtccttcatc agtcaagtgg tagatctttt tttgagagtg ctcgtctttg tccaaaacgg
     3241 tcacataacc ctgctggtct aacttggcaa gcgttgtata gatctgactg tgatgggctt
     3301 cccaaaaaat tgtgatcagc tgcttcaatt catacccaga acgtggtttg gataaaagca
     3361 cgcataataa tatataggct aaagtattca taaaaatcct acctctcgtc ttattttctt
     3421 tattatatca cagtaaagtt tccacg
//
LOCUS       DAKBVG010000477         3398 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.guided.222, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000477 DAKBVG010000000
VERSION     DAKBVG010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.guided.222"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            188..685
                     /gene="dfrG"
                     /locus_tag="OUD09_002884"
     CDS             188..685
                     /gene="dfrG"
                     /locus_tag="OUD09_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HCT9694062.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(757..2709)
                     /locus_tag="OUD09_002885"
     CDS             complement(757..2709)
                     /locus_tag="OUD09_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HCT9694063.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2716..2952)
                     /locus_tag="OUD09_002886"
     CDS             complement(2716..2952)
                     /locus_tag="OUD09_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT9694064.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            complement(3187..>3398)
                     /locus_tag="OUD09_002887"
     CDS             complement(3187..>3398)
                     /locus_tag="OUD09_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCT9694065.1"
                     /translation="ELEQEESIRFQNCSLFPLYHGSAKSNIGIDNLIEVITNKFYSST
                     HRGPSELCGNVFKIEYTKKRQRLAY"
BASE COUNT         1046 a          588 c          560 g         1204 t
ORIGIN      
        1 tacaaaatga tttataaaat gcaattgaaa atgataattg aatgaaaatt tgtgattttg
       61 tggtattatg ataatataac ttatagaaaa aattgcttat tcaactaacg ggcaggataa
      121 tgagaaaagg ttttttgata gaaataagaa attagatggt aaattagtta aaaaggctgt
      181 gttataaatg aaagtttctt tgattgctgc gatggataag aatagagtga ttggcaaaga
      241 gaatgacatt ccttggagga ttcccaagga ctgggaatat gttaaaaata ctacaaaggg
      301 acatccgata atattaggta ggaagaacct tgaatcaatc ggaagagcct tacctgacag
      361 aagaaatatt attctgacga gagataaggg gtttaccttt aatggttgtg aaattgttca
      421 ttcaatagaa gatgtttttg agttatgtaa aaacgaagaa gaaattttta ttttcggagg
      481 agaacagatt tataatttgt ttttccctta tgttgagaaa atgtacatca caaaaataca
      541 tcatgaattc gaaggagata ctttttttcc agaagtgaat tatgaggaat ggaatgaggt
      601 atttgcccaa aaagggataa agaatgataa aaatccgtat aactactatt ttcatgtata
      661 tgaaagaaaa aacttattga gttaacgggt gcgttaattg aagaagaaca gtaaagataa
      721 agcacaagag cttgggatta ctccaagctc tttttgtcat ttgaaataaa ttatatcttt
      781 ccaatcaaat tgtttatctg taatccctaa tctttgagca ggtgtcaaaa cctttttatc
      841 cgctgattta aaagggcgac aaaagttgta gtaagttctt agaattgtaa tcgcaaattg
      901 agcatacttc ggattgaagt tagcgtaaat atagctcttc ttatcaccac gagctgttgt
      961 taatggtctt tcaagagatg atatataacg tcttatctgt tgaataaagc tattggtcga
     1021 atggtcatta acatttaata gcatctttgc gatttcgttt ggttccagag cagataaatc
     1081 cgttctacaa tcaactgaat aaaatccttt atccttagtg gctaatggat gtttgatagg
     1141 gttatcggca tatttgcgat atctttctcc atctttacta atagcctctt cgtggaattg
     1201 atgacttgtt agtaattcag ttagataatg agatgccaaa gtccgtagaa actttgtttt
     1261 gaatcctctg atatctcccc aatcgagcag ttctgctttt gcttgtccga attcttttaa
     1321 gcattgtttt ctgcttttcg tcttgttaac ttggcaaata aaatgatgtg catcgctcaa
     1381 ccttaattct ttcgtgaata ctctataaaa ggcgtttcta atagagaagt catcatcact
     1441 aatcattcgc cattcgtcgg cattaaccat ttgtttaatt aaccaatagt gagcagttgt
     1501 agtataagga gcatcaacat ttaaaccatc gacaaatcta ctcctattag taattttccc
     1561 taattcgtga agataagcat tcctattttc gctatcgtta gcggatggtt cttgtggata
     1621 ataagaccaa tctaacctat catttttacg actaaaagta ttgagatggt cttctttgaa
     1681 ttttcttgta tcttcattta attcatccat tgaaatattc caatcatagg caacatcaga
     1741 acggaacaca tatcttgata agacctcagc agatacaact atatacgttt gcatattaag
     1801 gtcttcaaac cccgtatatt tctttgaacc ttgtcctttc ttacggacgt tatttaggta
     1861 gtagtgcatc ttgtccgtat taagccacat ctcgttaaat ttctttgttt gaaggggttg
     1921 tgtctcatat cgttctaaaa actccaaaca acgcctatac agccactcca gcttatgata
     1981 gtaggttcca actccaattc ctaaaatatc acaagtacga ctgactgaaa cccttccaac
     2041 aaccatctta gcgaacattg gaagaatggt gttcttttgt tggtgataag tagtggtttc
     2101 ttctcttttt ggtagaacat tagtgaactt cttacacgcc ttacattgat agcgttgaga
     2161 tttgccccta cttttccccc ttttgtaaaa ttgcttcggc tcattgaatg gggttgattc
     2221 ttcttccgag cagccttctt tatgaaattg ataatctggc tcaacatctt ggatgctatt
     2281 gatttctata aggcgtttaa tttcttcaac aacagaccaa ttagataaag gggttactgt
     2341 acagttttgc gatacacctc tgccaatagg attgtcattg cagtacatac ctttatgacc
     2401 tttatctttg gaactaccag tcattttata tcgacttggt ttccctttta catctttgaa
     2461 tttgtgctgt tctttcccaa aattacaaca aaacggattg atgcagtaat tatattgaat
     2521 gggatattca gaaccgttcc atttaaaaga cgttggcaag aacaacattt tgttatacct
     2581 tgttgcaaat cgttttggag ttaataaatc atattgttta gccctatctt ttaattcttc
     2641 gtttgaaatc ggaattttaa catctacaat ttcatcttcc ttcgtggcta atcttttaag
     2701 cttggtcatt gtctatcaac ccactctatc tttgataccc attttttcaa caatccgctt
     2761 attacttcgc ttgttggcaa tccattcaat aaacttctta tcgtctaaaa tgttcaataa
     2821 gaattcatcc actgcttcgc tttcagtacg ctctgcatat tcagcgtatt gcttaactat
     2881 cacccttact tgctctgata tttcccaatc cacagactta gcgttcttat tttttggctc
     2941 aagaaattgc ataggaaaat gcaccccttt ttgcttagta aattgtaacc attttaacac
     3001 accaatttgc attcctacaa taattatttc acgttcgcat ttttatatac attttaactt
     3061 acattttgtg gtgtaaatta acaacaaacg ttgatttaac aataaaaaac gagtatcaaa
     3121 ttcagttgca atttgatact catttaggtt tacattttca ctttttgtac tccaaaactg
     3181 aactacttaa tatgcaagac gttgtctttt ttttgtatat tcaattttga aaacatttcc
     3241 gcaaagttca gacggacctc gatgtgttga tgaataaaat ttattagtaa taacttctat
     3301 aaggttatca atccctatat tactttttgc acttccatga taaagaggga acagagaaca
     3361 attctgaaat cttatgcttt cctcttgttc gagttcca
//
LOCUS       DAKBVG010000478         2099 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.guided.275, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000478 DAKBVG010000000
VERSION     DAKBVG010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.guided.275"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            15..1517
                     /gene="eat(A)"
                     /locus_tag="OUD09_002888"
     CDS             15..1517
                     /gene="eat(A)"
                     /locus_tag="OUD09_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HCT9694066.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKSQPKHLSISYVRQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            1584..>2099
                     /locus_tag="OUD09_002889"
     CDS             1584..>2099
                     /locus_tag="OUD09_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCT9694067.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELV"
BASE COUNT          740 a          375 c          466 g          518 t
ORIGIN      
        1 cgaggtgatg gaaaatgtct aaaatcgaaa taaaaaatct gacattcggc tacgacagcc
       61 aaggcacatt attatttgaa caagcaaatc taaattttga cacacaatgg aaactaggac
      121 ttatcggacg aaacggtcga ggaaagacaa ctttactgaa tattctacaa aacaaactac
      181 cttatcaagg gcaagtaatc catcagcaag aatttgccta tttcccgcaa cagacaaaag
      241 ataaagaacg tttaacctat tacgtgttaa atgatattac ggattttgag atatgggaaa
      301 tcgaaagaga gctccaattg atgcaaacag atcctgaaat cttatggaga gaattcagca
      361 cactatcggg gggagagaag acaaaagtcc tactggcact tttatttgtg gatgacactc
      421 atttcccgtt aatcgatgaa ccaacgaatc atttggatat ctctggtaga aaacaagtag
      481 cggcttattt gaaaaagaaa aaacaaggct tcatcgtggt cagccatgac cggggattta
      541 tcgatgaagt agtggaccat gttttagcaa tcgaaaaaag tcaactggaa ctttatcaag
      601 ggaatttctc tatctatgaa gaacagaaaa aacttcgtga tgaatttgaa atggctcaaa
      661 atgaaaaatt gaaaaaagaa gtcagtaggc taaagaaaac agcagctgaa aaagccgaat
      721 ggtctcgttc ccgagaagga gataaaacaa agaaacaagt cggattcatc gatactgaat
      781 ctagacgagt gaataaagga gcagtgggtg ctgatgctgc acggacgatg aaacgatcca
      841 aagcaatcgt gaatcggatg gagacccaga tcagcgagaa agaaaaacta ttaaaagata
      901 tcgaatatat cgattctttg acgatgaata gccaagcgtc tcaccataag cgacttttaa
      961 gcgtagaaga tcttcaatta gggtatgaaa atctgttatt cgagccaatt cattttacaa
     1021 tcgagcctca tcagcgggtg gcgatttcag gtcctaacgg tgcaggaaag tcatccatta
     1081 tccattatct tctgggggca ttcaacggca aggttatagg agaaaaatcc cagccaaaac
     1141 atctgagcat tagttatgta cgccaaaatt atgaagacaa tcgaggaacg ttggcggaat
     1201 ttgcagagaa aaaccaagta gactaccaag catttttgaa caacctccga aagcttggga
     1261 tggaaagaga tgtttttcat aacaagatcg agcagatgag tatgggccaa cggaaaaaag
     1321 tggaattggc taaatcttta tcacagccag ctgaactata tacatgggat gaaccattga
     1381 attatttgga tgtcttcaat caagaacaat tagaacaact gatcttgaac gtgaaacctg
     1441 ccatgttact agtggaacat gatcaaacct tcctggataa agtatctact gagattattt
     1501 ctcttgagag aatctaaccc ttgtaatctc aagaacaaca aggtatggtt aaggggaaat
     1561 ttgccaacga aggagagaat cacatgcttt atgtaacgag acacggagaa acaacttgga
     1621 acgcacaagg attagtttgc ggacgggcag atgtgccgct gactgaaaaa ggacagatgc
     1681 aggcacagaa actagcagaa aaggtagtag atttgcctgt tccaatcacg aaaatcatcc
     1741 attcccctct tcaacgagca agagatacag cacaagcagt tgctgatcgt ctcagcttgc
     1801 cgctgaccgt tgatgaacga ttggtagaaa tggattttgg ggattatgat ggactgccaa
     1861 gcaaagatga gaactttcaa aaagcacgtt tagcgttcgc tgtacgattc ccaaatgggg
     1921 agtcagtctt agatgtgtat gcacggatcg ttcctttatt gaaagaatgc atcgaagacg
     1981 aagaaaatgt ctatctgtta gtttgtcata atgccttgat aagagtgatc aatgcttatt
     2041 ttcatccgat gcctaatgag gggttcttta cattcatggt agataataca gaattggtt
//
LOCUS       DAKBVG010000479         1963 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.guided.285, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000479 DAKBVG010000000
VERSION     DAKBVG010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.guided.285"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="OUD09_002890"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="OUD09_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HCT9694068.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIV
                     NTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRL
                     NSRIDKVRYMFNKIT"
BASE COUNT          673 a          308 c          407 g          575 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcaggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtac ggtttatact atagccctgt tagtactcca
     1561 gcagattttc ggatgcttac tcctattgta ctggagcaag cctttagaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa gtttatgcac cacaggaata tctttcacgg
     1681 gcatataacg atgctcccaa atattgtgca aatatcgtaa atactcaact gaaaaataat
     1741 gaggtcatta ttattggaga aattcctgct cgatgtattc aagattatcg caatgattta
     1801 acttttttta caaatgggct tagtgtttgt ttagcagagc taaaaggata tcaggttacc
     1861 actggcgaac ctgtttgcca gacccgtcgt ctaaatagtc ggatagataa agtaagatat
     1921 atgttcaata aaataactta gtgcgtttta tgttgttata taa
//
LOCUS       DAKBVG010000480         1963 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.guided.286, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000480 DAKBVG010000000
VERSION     DAKBVG010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.guided.286"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="OUD09_002891"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="OUD09_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HCT9694069.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIV
                     NTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRL
                     NSRIDKVRYMFNKIT"
BASE COUNT          672 a          308 c          408 g          575 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcgggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtac ggtttatact atagccctgt tagtactcca
     1561 gcagattttc ggatgcttac tcctattgta ctggagcaag cctttagaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa gtttatgcac cacaggaata tctttcacgg
     1681 gcatataacg atgctcccaa atattgtgca aatatcgtaa atactcaact gaaaaataat
     1741 gaggtcatta ttattggaga aattcctgct cgatgtattc aagattatcg caatgattta
     1801 acttttttta caaatgggct tagtgtttgt ttagcagagc taaaaggata tcaggttacc
     1861 actggcgaac ctgtttgcca gacccgtcgt ctaaatagtc ggatagataa agtaagatat
     1921 atgttcaata aaataactta gtgcgtttta tgttgttata taa
//
LOCUS       DAKBVG010000481         1941 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.guided.289, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000481 DAKBVG010000000
VERSION     DAKBVG010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.guided.289"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="OUD09_002892"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="OUD09_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HCT9694070.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
BASE COUNT          666 a          311 c          405 g          559 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcaggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1861 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1921 atgttcaata aaataactta g
//
LOCUS       DAKBVG010000482         1941 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.guided.290, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000482 DAKBVG010000000
VERSION     DAKBVG010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.guided.290"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="OUD09_002893"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="OUD09_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HCT9694071.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
BASE COUNT          665 a          311 c          406 g          559 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcgggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1861 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1921 atgttcaata aaataactta g
//
LOCUS       DAKBVG010000483         1512 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.guided.319, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000483 DAKBVG010000000
VERSION     DAKBVG010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.guided.319"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            29..1402
                     /gene="tet(L)"
                     /locus_tag="OUD09_002894"
     CDS             29..1402
                     /gene="tet(L)"
                     /locus_tag="OUD09_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HCT9694072.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
BASE COUNT          432 a          224 c          305 g          551 t
ORIGIN      
        1 gaattaaagt gtaaaggagg aattaattat gaagtgtaat gaatgtaaca gggttaaata
       61 caaccaaatt ttaatttggc tttgcgtttt atcttttttt agcgtattaa atgaaatggt
      121 tttgaacgtc tcattacctg atattgcaaa tgattttaat aaaccacctg cgagtacaaa
      181 ctgggtgaac acagccttta tgttaacctt ttccattgga acagctgtat atggaaagct
      241 atctgatcaa ttaggcatca aaaggttact cctatttgga attataataa attgtttcgg
      301 gtcggtaatt gggtttgttg gccattcttt cttttcctta cttattatgg ctcgttttat
      361 tcaaggggct ggtgcagctg catttccagc actcgtaatg gttgtagttg cgcgctatat
      421 tccaaaggaa aataggggta aagcatttgg tcttattgga tcgatagtag ccatgggaga
      481 aggagtcggt ccagcgattg gtggaatgat agcccattat attcattggt cctatcttct
      541 actcattcct atgataacaa ttatcactgt tccgtttctt atgaaattat taaagaaaga
      601 agtaaggata aaaggtcatt ttgatatcaa aggaattata ctaatgtctg taggcattgt
      661 attttttatg ttgtttacaa catcatatag catttctttt cttatcgtta gcgtgctgtc
      721 attcctgata tttgtaaaac atatcaggaa agtaacagat ccttttgttg atcccggatt
      781 agggaaaaat atacctttta tgattggagt tctttgtggg ggaattatat ttggaacagt
      841 agcagggttt gtctctatgg ttccttatat gatgaaagat gttcaccagc taagtactgc
      901 cgaaatcgga agtgtaatta ttttccctgg aacaatgagt gtcattattt tcggctacat
      961 tggtgggata cttgttgata gaagaggtcc tttatacgtg ttaaacatcg gagttacatt
     1021 tctttctgtt agctttttaa ctgcttcctt tcttttagaa acaacatcat ggttcatgac
     1081 aattataatc gtatttgttt taggtgggct ttcgttcacc aaaacagtta tatcaacaat
     1141 tgtttcaagt agcttgaaac agcaggaagc tggtgctgga atgagtttgc ttaactttac
     1201 cagcttttta tcagagggaa caggtattgc aattgtaggt ggtttattat ccataccctt
     1261 acttgatcaa aggttgttac ctatggaagt tgatcagtca acttatctgt atagtaattt
     1321 gttattactt ttttcaggaa tcattgtcat tagttggctg gttaccttga atgtatataa
     1381 acattctcaa agggatttct aaatcgttaa gggatcaact ttgggagaga gttcaaaatt
     1441 gatccttttt ttataacagg aattcaaatc tttttgttcc attaaagggc gcgattgctg
     1501 aataaaagat ac
//
LOCUS       DAKBVG010000484         1270 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.guided.335, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000484 DAKBVG010000000
VERSION     DAKBVG010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.guided.335"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            208..1248
                     /locus_tag="OUD09_002895"
     CDS             208..1248
                     /locus_tag="OUD09_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCT9694073.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
BASE COUNT          421 a          194 c          247 g          408 t
ORIGIN      
        1 cattttcaat tgcattttat aaatcatttt gtatgtaatt tggtatgtaa gttaaaatga
       61 aaaaagtagt ccattttgaa gtacattttg tcattttgta ctccaaaact gaactactta
      121 cttgcatata tacgccttta tagtggagta ctacatttac gagattcggt tagagtatca
      181 gaaaaagaaa aaataaaagt tacagaaatg tatacttcaa taaatggtga attatgtaag
      241 attgatagag cttattctgg agaaattgtt attttgcaaa atgagttttt gaagttaaat
      301 agtgttcttg gagatacaaa actattgcca cagagaaaaa agattgaaaa tccgcaccct
      361 ctactacaaa caactgttga accgagtaaa cctgaacaga gagaaatgtt gcttgatgcc
      421 cttttggaaa tctcagatag tgatccgctt ctacgatatt acgtggattc tacgacacat
      481 gaaattatac tttctttctt agggaaagta caaatggaag tgattagtgc actgttgcaa
      541 gaaaagtatc atgtggagat agaactaaaa gagcctacag tcatttatat ggagagaccg
      601 ttaaaaaatg cagaatatac cattcacatc gaagtgccgc caaatccttt ctgggcttcc
      661 attggtttat ctgtatcacc gcttccgttg ggaagtggaa tgcagtatga gagctcggtt
      721 tctcttggat acttaaatca atcatttcaa aatgcagtta tggaagggat acgctatggt
      781 tgcgaacaag gattatatgg ttggaatgtg acggattgta aaatctgttt taagtacggt
      841 ttatactata gccctgttag tactccagca gattttcgga tgcttactcc tattgtactg
      901 gagcaagcct ttagaaaagc tggaacagaa ttgttagagc catatcttag ttttaaagtt
      961 tatgcaccac aggaatatct ttcacgggca tataacgatg ctcccaaata ttgtgcaaat
     1021 atcgtaaata ctcaactgaa aaataatgag gtcattatta ttggagaaat tcctgctcga
     1081 tgtattcaag attatcgcaa tgatttaact ttttttacaa atgggcttag tgtttgttta
     1141 gcagagctaa aaggatatca ggttaccact ggcgaacctg tttgccagac ccgtcgtcta
     1201 aatagtcgga tagataaagt aagatatatg ttcaataaaa taacttagtg cgttttatgt
     1261 tgttatataa
//
LOCUS       DAKBVG010000485         1248 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.guided.336, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000485 DAKBVG010000000
VERSION     DAKBVG010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.guided.336"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            208..1248
                     /locus_tag="OUD09_002896"
     CDS             208..1248
                     /locus_tag="OUD09_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetM/TetW/TetO/TetS family tetracycline
                     resistance ribosomal protection protein"
                     /protein_id="HCT9694074.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIVDTQ
                     LKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRPNSR
                     IDKVRYMFNKIT"
BASE COUNT          414 a          197 c          245 g          392 t
ORIGIN      
        1 cattttcaat tgcattttat aaatcatttt gtatgtaatt tggtatgtaa gttaaaatga
       61 aaaaagtagt ccattttgaa gtacattttg tcattttgta ctccaaaact gaactactta
      121 cttgcatata tacgccttta tagtggagta ctacatttac gagattcggt tagagtatca
      181 gaaaaagaaa aaataaaagt tacagaaatg tatacttcaa taaatggtga attatgtaag
      241 attgatagag cttattctgg agaaattgtt attttgcaaa atgagttttt gaagttaaat
      301 agtgttcttg gagatacaaa actattgcca cagagaaaaa agattgaaaa tccgcaccct
      361 ctactacaaa caactgttga accgagtaaa cctgaacaga gagaaatgtt gcttgatgcc
      421 cttttggaaa tctcagatag tgatccgctt ctacgatatt acgtggattc tacgacacat
      481 gaaattatac tttctttctt agggaaagta caaatggaag tgattagtgc actgttgcaa
      541 gaaaagtatc atgtggagat agaactaaaa gagcctacag tcatttatat ggagagaccg
      601 ttaaaaaatg cagaatatac cattcacatc gaagtgccgc caaatccttt ctgggcttcc
      661 attggtttat ctgtatcacc gcttccgttg ggaagtggaa tgcagtatga gagctcggtt
      721 tctcttggat acttaaatca atcatttcaa aatgcagtta tggaagggat acgctatggt
      781 tgcgaacaag gattatatgg ttggaatgtg acggattgta aaatctgttt taagtatggc
      841 ttatactata gccctgttag taccccagca gattttcgga tgcttgctcc tattgtattg
      901 gaacaagtct taaaaaaagc tggaacagaa ttgttagagc catatcttag ttttaaaatt
      961 tatgcgccac aggaatatct ttcacgagca tacaacgatg ctcctaaata ttgtgcgaac
     1021 atcgtagaca ctcaattgaa aaataatgag gtcattctta gtggagaaat ccctgctcgg
     1081 tgtattcaag aatatcgtag tgatttaact ttctttacaa atggacgtag tgtttgttta
     1141 acagagttaa aagggtacca tgttactacc ggtgaacctg tttgccagcc ccgtcgtcca
     1201 aatagtcgga tagataaagt acgatatatg ttcaataaaa taacttag
//
LOCUS       DAKBVG010000486          919 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.guided.368, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000486 DAKBVG010000000
VERSION     DAKBVG010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.guided.368"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            306..854
                     /gene="aac(6')"
                     /locus_tag="OUD09_002897"
     CDS             306..854
                     /gene="aac(6')"
                     /locus_tag="OUD09_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HCT9694075.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          333 a          130 c          187 g          269 t
ORIGIN      
        1 taaaaattct aaaaattaag attattatat gaaaacaggt tttatttgtc aattcataat
       61 tggttttttt taaagcaaaa gataatctat atcatgatga acaaacagag ttttttcctc
      121 caattgatgg ctttcctgat ctttcctatt ttgaataaag tgtttctgta taattttttt
      181 attttataag taagataaat aatgaaagaa tagagatact gattggtagc taaagatttt
      241 tgagcgagaa catttcgttt tttcaaaagg actgttagaa taagcaagaa caagggggaa
      301 gacgtatgat aatcagtgaa tttgaccgta ataatccagt attgaaagat cagctttctg
      361 atttactgag actgacttgg ccggaagaat atggagacag ctcggcagaa gaagtagaag
      421 aaatgatgaa tccagaacga atcgcggtag cagcggtaga ccaagatgag ttagtaggat
      481 ttattggtgc aatccctcaa tacggtatca caggttggga attgcatcca ttagttgtag
      541 aaagctcccg acgaaagaac caaataggta ctcgattagt caattactta gaaaaagaag
      601 tagcttccag aggaggaatc acgatttatt taggtacgga tgatttagac catggaacaa
      661 cgttaagtca aacggacctg tatgagcata catttgataa agtggcttcc atccagaacc
      721 ttcgtgaaca tccgtatgaa ttctatgaaa aattaggtta taaaatcgta ggtgtcttac
      781 caaatgcaaa tggctgggac aaaccggata tttggatggc aaaaacgatt attcctcgac
      841 caaattctca ataataaaaa gaggttgtga cagaagtaat cagcttcaag aaataagaag
      901 gaatttacga aaattactt
//
LOCUS       DAKBVG010000487          870 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.guided.377, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000487 DAKBVG010000000
VERSION     DAKBVG010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..870
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.guided.377"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            32..766
                     /gene="erm"
                     /locus_tag="OUD09_002898"
     CDS             32..766
                     /gene="erm"
                     /locus_tag="OUD09_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011282421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S ribosomal RNA methyltransferase Erm"
                     /protein_id="HCT9694076.1"
                     /translation="MNKKNIKDSQNFITSKHHINEILRNVHLNTNDNIIEIGSGKGHF
                     TFELAKRCNYVTAIEIDPKLCRITKNKLIEYENFQVINKDILQFKFPKNKSYKIYGNI
                     PYNISTDIIRKIVFESTATESYLIVEYGFAKRLLNTNRSLALFLMTEVDISILSKIPR
                     EYFHPKPRVNSSLIVLKRHPSKISLKDRKQYENFVMKWVNKEYTKLFSKNQFYQALKY
                     ARIDDLNNISFEQFLSLFNSYKLFNR"
BASE COUNT          353 a          119 c          117 g          281 t
ORIGIN      
        1 attcattata acctcatagg agtgggttat aatgaacaaa aaaaatataa aagatagtca
       61 aaactttatt acttcaaagc atcatataaa tgaaattttg agaaatgtac atttaaatac
      121 aaatgataat attattgaga ttggttcagg gaaaggtcat ttcacgtttg aattagctaa
      181 aaggtgtaat tatgtaaccg ccattgaaat agatcctaaa ttatgtagga taactaaaaa
      241 caaacttatt gaatatgaga acttccaggt tatcaataaa gatattttac aatttaagtt
      301 tcctaaaaat aagtcatata agatatatgg aaatataccc tacaatataa gtacagatat
      361 aattcgaaaa attgtttttg aaagcacagc tacagaaagt tatttaatag tggaatatgg
      421 atttgctaaa aggttgctaa atacaaatcg ttcactagca ctatttttaa tgacagaagt
      481 tgatatatcc atattaagta aaatccctag agaatacttt catccaaaac ctagagttaa
      541 tagctcgtta attgtattaa aaagacaccc ttcaaaaata tcactcaaag atagaaaaca
      601 atatgaaaat tttgttatga aatgggttaa caaagaatac acaaaactat tttccaaaaa
      661 ccaattttat caagccttaa aatatgcaag aattgacgat ttaaacaata ttagctttga
      721 acaattcttg tctcttttca atagctataa attatttaat agataagtta aggggtgcat
      781 aaactgcatc ccttaactta tcttattttg cttaaacaat cttggcccgt aaactgcata
      841 actcatatgt aaatcgctcc tttttaggtg
//
LOCUS       DAKBVG010000488          870 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.guided.378, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000488 DAKBVG010000000
VERSION     DAKBVG010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..870
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.guided.378"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            32..766
                     /locus_tag="OUD09_002899"
     CDS             32..766
                     /locus_tag="OUD09_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011282421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(T)"
                     /protein_id="HCT9694077.1"
                     /translation="MNKKNIKDSQNFITSKHHINEILRNVHLNTNDNIIEIGSGKGHF
                     TFELAKRCNYVTAIEIDPKLCRITKNKLIEYENFQVINKDILQFKFPKNKSYKIYGNI
                     PYNISTDIIRKIVFESTATESYLIVEYGFAKRLLNTNRSLALFLMTEVDISILSKIPR
                     EYFHPKPRVNSSLIVLKRHPSKISLKDRKQYENFVMKWVNKEYIKLFSKNQFYQALKY
                     ARIDDLNNISFEQFLSLFNSYKLFNR"
BASE COUNT          353 a          118 c          117 g          282 t
ORIGIN      
        1 attcattata acctcatagg agtgggttat aatgaacaaa aaaaatataa aagatagtca
       61 aaactttatt acttcaaagc atcatataaa tgaaattttg agaaatgtac atttaaatac
      121 aaatgataat attattgaga ttggttcagg gaaaggtcat ttcacgtttg aattagctaa
      181 aaggtgtaat tatgtaaccg ccattgaaat agatcctaaa ttatgtagga taactaaaaa
      241 caaacttatt gaatatgaga acttccaggt tatcaataaa gatattttac aatttaagtt
      301 tcctaaaaat aagtcatata agatatatgg aaatataccc tacaatataa gtacagatat
      361 aattcgaaaa attgtttttg aaagcacagc tacagaaagt tatttaatag tggaatatgg
      421 atttgctaaa aggttgctaa atacaaatcg ttcactagca ctatttttaa tgacagaagt
      481 tgatatatcc atattaagta aaatccctag agaatacttt catccaaaac ctagagttaa
      541 tagctcgtta attgtattaa aaagacaccc ttcaaaaata tcactcaaag atagaaaaca
      601 atatgaaaat tttgttatga aatgggttaa caaagaatac ataaaactat tttccaaaaa
      661 ccaattttat caagccttaa aatatgcaag aattgacgat ttaaacaata ttagctttga
      721 acaattcttg tctcttttca atagctataa attatttaat agataagtta aggggtgcat
      781 aaactgcatc ccttaactta tcttattttg cttaaacaat cttggcccgt aaactgcata
      841 actcatatgt aaatcgctcc tttttaggtg
//
LOCUS       DAKBVG010000489          856 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.guided.383, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000489 DAKBVG010000000
VERSION     DAKBVG010000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..856
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.guided.383"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            38..>856
                     /gene="aac(6')-Ie"
                     /locus_tag="OUD09_002900"
     CDS             38..>856
                     /gene="aac(6')-Ie"
                     /locus_tag="OUD09_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ie"
                     /protein_id="HCT9694078.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILG"
BASE COUNT          363 a           64 c          146 g          283 t
ORIGIN      
        1 agtatatgta tatgattatg aaaaaggtga taaataaatg aatatagttg aaaatgaaat
       61 atgtataaga actttaatag atgatgattt tcctttgatg ttaaaatggt taactgatga
      121 aagagtatta gaattttatg atggtagaga taaaaaatat acattagaat cattaaaaaa
      181 acattataca gagccttggg aagatgaagt ttttagagta attattgaat ataacaatgt
      241 tcctattgga tatggacaaa tatataaaat gtatgatgag ttatatactg attatcatta
      301 tccaaaaact gatgagatag tctatggtat ggatcaattt ataggagagc caaattattg
      361 gagtaaagga attggtacaa gatatattaa attgattttt gaatttttga aaaaagaaag
      421 aaatgctaat gcagttattt tagaccctca taaaaataat ccaagagcaa taagggcata
      481 ccaaaaatct ggttttagaa ttattgaaga tttgccagaa catgaattac acgagggcaa
      541 aaaagaagat tgttatttaa tggaatatag atatgatgat aatgccacaa atgttaaggc
      601 aatgaaatat ttaattgagc attactttga taatttcaaa gtagatagta ttgaaataat
      661 cggtagtggt tatgatagtg tggcatattt agttaataat gaatacattt ttaaaacaaa
      721 atttagtact aataagaaaa aaggttatgc aaaagaaaaa gcaatatata attttttaaa
      781 tacaaattta gaaactaatg taaaaattcc taatattgaa tattcgtata ttagtgatga
      841 attatctata ctaggt
//
LOCUS       DAKBVG010000490          856 bp    DNA     linear   BCT 24-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate
            DTU_2021_1003097_1_SI_THA_BAN_001
            SAMEA111506266-rid15940123.guided.384, whole genome shotgun
            sequence.
ACCESSION   DAKBVG010000490 DAKBVG010000000
VERSION     DAKBVG010000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111506266
            Sequence Read Archive: ERR10432704
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493678.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493678.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 08:59:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,763
            CDSs (with protein)               :: 2,763
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 28 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 7 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..856
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111506266-rid15940123.guided.384"
                     /isolate="DTU_2021_1003097_1_SI_THA_BAN_001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-11-16"
                     /collected_by="Hospital of Tropical Diseases -
                     Microbiology Department"
     gene            38..>856
                     /gene="aac(6')-Ie"
                     /locus_tag="OUD09_002901"
     CDS             38..>856
                     /gene="aac(6')-Ie"
                     /locus_tag="OUD09_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ie"
                     /protein_id="HCT9694079.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYGGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILG"
BASE COUNT          362 a           64 c          147 g          283 t
ORIGIN      
        1 agtatatgta tatgattatg aaaaaggtga taaataaatg aatatagttg aaaatgaaat
       61 atgtataaga actttaatag atgatgattt tcctttgatg ttaaaatggt taactgatga
      121 aagagtatta gaattttatg gtggtagaga taaaaaatat acattagaat cattaaaaaa
      181 acattataca gagccttggg aagatgaagt ttttagagta attattgaat ataacaatgt
      241 tcctattgga tatggacaaa tatataaaat gtatgatgag ttatatactg attatcatta
      301 tccaaaaact gatgagatag tctatggtat ggatcaattt ataggagagc caaattattg
      361 gagtaaagga attggtacaa gatatattaa attgattttt gaatttttga aaaaagaaag
      421 aaatgctaat gcagttattt tagaccctca taaaaataat ccaagagcaa taagggcata
      481 ccaaaaatct ggttttagaa ttattgaaga tttgccagaa catgaattac acgagggcaa
      541 aaaagaagat tgttatttaa tggaatatag atatgatgat aatgccacaa atgttaaggc
      601 aatgaaatat ttaattgagc attactttga taatttcaaa gtagatagta ttgaaataat
      661 cggtagtggt tatgatagtg tggcatattt agttaataat gaatacattt ttaaaacaaa
      721 atttagtact aataagaaaa aaggttatgc aaaagaaaaa gcaatatata attttttaaa
      781 tacaaattta gaaactaatg taaaaattcc taatattgaa tattcgtata ttagtgatga
      841 attatctata ctaggt
//
